A novel p53 mutant in human breast cancer revealed by multiple SSCP analysis.
DNA from tumor tissue and peripheral blood lymphocytes of primary breast cancer patients was screened for the presence of p53 mutations. In DNA from one tumor we found that the histidine codon 193 (CAT) was somatically converted to arginine (CGT). This amino acid residue is highly conserved in many species, thus suggesting that such mutation plays an important role in the loss of wt-p53 function.
Nigro
V
V
Istituto di Patologia Generale e Oncologia, Facoltà di Medicina, Seconda Università degli Studi di Napoli, Italy.
Napolitano
M
M
Abbondanza
C
C
Medici
N
N
Puca
A A
AA
Schiavulli
M
M
Armetta
I
I
Moncharmont
B
B
Puca
G A
GA
Molinari
A M
AM
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002645:Chickens; D003062:Codon; D017124:Conserved Sequence; D017931:DNA Primers; D005091:Exons; D005260:Female; D005784:Gene Amplification; D016158:Genes, p53; D000882:Haplorhini; D006639:Histidine; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014337:Trout; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000235:genetics*; D014981:Xenopus
D003062:Codon; D017931:DNA Primers; D016159:Tumor Suppressor Protein p53; D006639:Histidine; D001120:Arginine