Polyvinylpyrrolidone-coated silica packings for chromatography of proteins and peptides.
Polyvinylpyrrolidone (PVP)-coated silica sorbents were synthesized by interaction of a copolymer of vinyl-methyldiethoxysilane and vinylpyrrolidone with LiChrospher Si 300 and LiChrospher Si 500 silicas. The coating procedure retains the wide-pore structure of the starting silicas, as was shown by nitrogen adsorption, mercury porosimetry and inverse size-exclusion chromatographic measurements. Good selectivity and separation ability of the synthesized packings toward proteins and peptides were demonstrated in the hydrophobic interaction chromatographic mode of separation. Aromatic compounds undergo a specific interaction with bonded PVP chains which can be used for the preconcentration or selective recovery of polycyclic aromatic hydrocarbons.
Kurganov
A
A
Institute of Organo-Element Compounds, Russian Academy of Sciences, Moscow.
Puchkova
Y u
Yu
Davankov
V
V
Eisenbeiss
F
F
D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011083:Polycyclic Compounds / Q000302:isolation & purification; D011137:Polystyrenes; D016062:Porosity; D011205:Povidone / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide
D010455:Peptides; D011083:Polycyclic Compounds; D011137:Polystyrenes; D011506:Proteins; D012822:Silicon Dioxide; D011205:Povidone