title: Is the IFN-gamma-induced enhancement of superoxide production in CGD-phagocytes caused by increased expression of the p47-phox cytosolic protein.
abstract: Interferon-gamma (IFN-gamma) had been shown to increase superoxide production of cultured monocytes from patients with chronic granulomatous disease (CGD). To elucidate the mechanism of the IFN-gamma-induced improvement of superoxide production of cultured monocytes from patients with CGD we examined the influence of IFN-gamma on the expression and the activity of the NADPH-oxidase in the monocytic cell-line Mono Mac 6. After cultivation of Mono Mac 6-cells in the presence of 500 U/ml IFN-gamma the superoxide production was found to be increased as well as the expression of the p47-phox cytosolic protein of the phagocytic NADPH-oxidase.
authors.affiliation: Universitätskinderklinik Göttingen.
authors.forename: V
authors.initials: V
authors.lastname: Jendrossek
authors.forename: U
authors.initials: U
authors.lastname: Damm
authors.forename: J
authors.initials: J
authors.lastname: Liese
authors.forename: B H
authors.initials: BH
authors.lastname: Belohradsky
authors.forename: M
authors.initials: M
authors.lastname: Gahr
mesh_terms: D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D005808:Genes, Recessive; D006105:Granulomatous Disease, Chronic / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000172:deficiency / Q000378:metabolism*; D019255:NADPH Oxidases; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010750:Phosphoproteins / Q000172:deficiency / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D010750:Phosphoproteins; D013481:Superoxides; D007371:Interferon-gamma; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D009252:NADPH Dehydrogenase