Identification of Neisseria gonorrhoeae in synovial fluid using the polymerase chain reaction.
OBJECTIVE
To analyze synovial fluid (SF) for the presence of Neisseria gonorrhoeae DNA using the polymerase chain reaction (PCR).


METHODS
We used a modified, nested PCR to detect the presence of N gonorrhoeae DNA in 41 samples of SF obtained from 10 patients with clinical gonococcal arthritis whose SF samples were sterile by culture and from 27 controls, including 11 patients with Reiter's syndrome. Results obtained using this method were compared with those obtained using the GEN-PROBE system, an RNA-DNA hybridization technique.


RESULTS
With nested PCR, N gonorrhoeae DNA was detected in 11 of 14 SF samples obtained from patients with culture-negative clinical gonococcal arthritis but in none of the 11 SF samples from Reiter's syndrome patients. The specificity of this technique was 96.4%, with a sensitivity of 78.6%. The rate of false-positive results was 3.6%. The GEN-PROBE technique was unable to detect N gonorrhoeae ribosomal RNA in any of the samples.


CONCLUSIONS
These findings demonstrate the potential utility of the PCR in confirming the clinical diagnosis of gonococcal arthritis as well as providing insight into the pathogenesis of this disorder in patients whose SF are sterile by standard culture techniques. PCR may also prove helpful in differentiating N gonorrhoeae arthritis from acute Reiter's syndrome.
Liebling
M R
MR
Division of Rheumatology, Harbor-UCLA Medical Center, Torrance, CA 90509.
Arkfeld
D G
DG
Michelini
G A
GA
Nishio
M J
MJ
Eng
B J
BJ
Jin
T
T
Louie
J S
JS
D000293:Adolescent; D000328:Adult; D001170:Arthritis, Infectious / Q000175:diagnosis*; D016918:Arthritis, Reactive / Q000175:diagnosis*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D003937:Diagnosis, Differential; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013582:Synovial Fluid / Q000382:microbiology*
D004269:DNA, Bacterial