A 43-kDa nuclear tobacco protein interacts with a specific single-stranded DNA sequence from the 5'-upstream region of the Agrobacterium rhizogenes rolC gene.
We identified in the nuclear extract of tobacco seedlings a 43-kDa DNA-binding protein RCS2 that interacted with the 5'-upstream region of the rolC gene of the Agrobacterium rhizogenes Ri plasmid. DNA-protein gel shift and competition assays demonstrated that RCS2 bound to single-stranded DNA in a sequence-specific manner. A five-base direct repeat is important for the DNA binding of RCS2. South-Western blot analysis was employed to determine the size of RCS2 which appears to be approx. 43 kDa.
Matsuki
R
R
Department of Biology, Faculty of Science, Hokkaido University, Sapparo, Japan.
Uchimiya
H
H
D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002467:Cell Nucleus / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012231:Rhizobium / Q000235:genetics*; D012639:Seeds; D014026:Nicotiana / Q000737:chemistry*
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins