BmCF1, a Bombyx mori RXR-type receptor related to the Drosophila ultraspiracle.
A PCR approach has been used to obtain an ovarian cDNA clone from the silkmoth Bombyx mori, encoding a 50 kDa protein (BmCF1) that belongs to the RXR subfamily of nuclear hormone receptors and is most similar to the CF1/USP protein (DmCF1) encoded by the ultraspiracle gene of Drosophila. The similarity is high in the DNA-binding and moderately so in the ligand-binding domains, although not in the N-terminal, putatively activator A/B domain. Protein sequence comparisons with the available RXR sequences indicate that although insect USP-like sequences are more related to each other than to vertebrate RXRs, their inter se similarities are lower than in the case of the vertebrate RXRs. Two distinct BmCF1-homologous transcripts are observed consistently, and are indicative either of alternative splicing or of the existence of a second RXR gene in the moth. The transcripts are widely distributed, suggesting functions at multiple developmental stages, as in the case of Drosophila ultraspiracle.
Tzertzinis
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Department of Cellular and Developmental Biology Harvard University, Cambridge, MA 02138.
Malecki
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A
Kafatos
F C
FC
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D004330:Drosophila / Q000737:chemistry / Q000235:genetics*; D029721:Drosophila Proteins; D005260:Female; D017344:Genes, Insect; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000235:genetics*; D018168:Receptors, Retinoic Acid; D011987:Receptors, Steroid / Q000737:chemistry / Q000235:genetics*; D047488:Retinoid X Receptors; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D018168:Receptors, Retinoic Acid; D011987:Receptors, Steroid; D047488:Retinoid X Receptors; D014157:Transcription Factors; C066030:USP protein, Drosophila; C087162:BmCF1 protein, Bombyx mori