title: Structure-activity relationship in the ability of flavonols to inhibit chemiluminescence.
abstract: Fourteen flavonoids were evaluated for their ability to inhibit chemiluminescence, either of neutrophils that had been briefly exposed to both luminol and phorbol-myristate acetate or to an enzymatic system with H2O2, luminol and horseradish peroxidase. Using chemiluminescence as the quantitative parameter, it can be concluded that the hydroxyl group in position 3 of the flavonols is vital for their inhibitory effect, and that two hydroxyl groups on the phenyl ring are optimal for such an effect. It was also noted that the C2-C3 double bond is essential for the flavonols' anti-oxidative effect. It is suggested that the ability of flavonols to suppress chemiluminescence is reciprocally correlated with their lipophilicity.
authors.affiliation: Department of Microbiology and Immunology, Silesian School of Medicine, Zabrze-Rokitnica, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Krol
authors.forename: Z
authors.initials: Z
authors.lastname: Czuba
authors.forename: S
authors.initials: S
authors.lastname: Scheller
authors.forename: Z
authors.initials: Z
authors.lastname: Paradowski
authors.forename: J
authors.initials: J
authors.lastname: Shani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D044948:Flavonols; D016166:Free Radical Scavengers; D006168:Guinea Pigs; D006735:Horseradish Peroxidase / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D044948:Flavonols; D016166:Free Radical Scavengers; D006735:Horseradish Peroxidase