Detection of Epstein-Barr virus DNA and EBV-determined nuclear antigen in angioimmunoblastic lymphadenopathy with dysproteinemia type T cell lymphoma.
Six cases of angioimmunoblastic lymphadenopathy with dysproteinemia (AILD)-like T cell lymphoma were analyzed by immunohistochemical staining, polymerase chain reaction (PCR) and Southern blot analysis. Five cases out of six showed gene rearrangements of the T cell receptor beta chain, indicating the existence of a clonal T cell proliferation. Epstein-Barr virus (EBV) DNA was detected in all six AILD type cases by PCR amplifying the sequence located in the internal repeat I, and confirmed by Southern blot hybridization, using a BamHI-W fragment as a probe. Detection of the EBV genome occurred more frequently as compared with other types of lymphoid disorders. Furthermore, EBV determined nuclear antigen (EBNA) was detected in UCHL-1 (CD45RO, pan-T cell marker) positive cells by immunostaining. These results suggest that a significant number of AILD type cases are T cell origin lymphomas and EBV infection may be relevant to the biological features of this type of lymphoma.
Kon
S
S
Department of Pathology, Sapporo Medical University, Japan.
Sato
T
T
Onodera
K
K
Satoh
M
M
Kikuchi
K
K
Imai
S
S
Osato
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D001796:Blood Protein Disorders / Q000382:microbiology*; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D006566:Herpesviridae Infections / Q000175:diagnosis*; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000382:microbiology*; D007124:Immunoenzyme Techniques; D017493:Leukocyte Common Antigens; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000175:diagnosis*
D000956:Antigens, Viral; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D017493:Leukocyte Common Antigens