Involvement of ribonuclease III in the enhancement of expression of the speF-potE operon encoding inducible ornithine decarboxylase and polyamine transport protein.
Since the speF-potE operon (pPT71 clone) encoding inducible ornithine decarboxylase (iODC) and polyamine transport potE protein is inducible at acidic pH, a gene encoding a protein involved in the enhancement of expression of the operon was searched for. Using the fused gene containing the upstream sequence of the speF-potE operon and the open reading frame of beta-galactosidase as a reporter gene, a clone (pPTS23) which causes the increase of beta-galactosidase activity at acidic pH was isolated. The clone also increased iODC activity at acidic pH and was identified as a gene encoding RNase III. This is the first example that RNase III increases the translational efficiency of mRNA derived from Escherichia coli gene by cutting the 5'-untranslated region of mRNA.
Kashiwagi
K
K
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Watanabe
R
R
Igarashi
K
K
D017920:Antiporters / Q000096:biosynthesis* / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004722:Endoribonucleases / Q000378:metabolism*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D009952:Ornithine / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D043244:Ribonuclease III; D001616:beta-Galactosidase / Q000378:metabolism
D017920:Antiporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C071519:PotE protein, E coli; D012333:RNA, Messenger; D009952:Ornithine; D004722:Endoribonucleases; D043244:Ribonuclease III; C468366:ribonuclease III, E coli; D001616:beta-Galactosidase; D009955:Ornithine Decarboxylase