[Synthesis of peptides in organic solvents catalyzed by pepsin].
The porcine pepsin immobilized on inorganic supports catalyzes the peptide bond formation in organic solvents. Dependence of the peptide bond formation between Z-Ala-Ala-Phe-OH and H-Leu-Ala-Ala-OCH3 upon the porous material, organic solvent, reaction time, enzyme concentration, ionic strength and pH was studied. Syntheses of peptides of the general formula Z-Ala-Ala-Xaa-Yaa-Ala-Ala-OCH3, where Xaa = Phe, Tyr, Trp; Yaa = Leu, Phe, Tyr, Trp, were carried out.
Anisimova
V V
VV
Lysogorskaia
E N
EN
Filippova
I Iu
IIu
Oksenoĭt
E S
ES
Stepanov
V M
VM
D000085:Acetates; D000097:Acetonitriles; D000595:Amino Acid Sequence; D000818:Animals; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D000431:Ethanol; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008752:Methylene Chloride; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis*; D009994:Osmolar Concentration; D010434:Pepsin A / Q000737:chemistry*; D012997:Solvents; D013552:Swine
D000085:Acetates; D000097:Acetonitriles; D004800:Enzymes, Immobilized; D009842:Oligopeptides; D012997:Solvents; D000431:Ethanol; D008752:Methylene Chloride; C007650:ethyl acetate; D010434:Pepsin A; C032159:acetonitrile
Kataliziruemyĭ pepsinom sintez peptidov v organicheskikh rastvoriteliakh.