title: Nitric oxide modulates water and electrolyte transport in the ileum.
abstract: OBJECTIVE
  To test the hypothesis that nitric oxide is a modulator of ileal water and ion transport.
  
  
  BACKGROUND
  Nitric oxide is produced in the vascular endothelium and enteric neural plexuses of the intestine and is involved in gastrointestinal motility and smooth muscle contractility. Little is known about the role of nitric oxide in intestinal epithelial transport.
  
  
  METHODS
  Ten-centimeter rabbit ileal segments (n = 50) were vascularly perfused with an electrolyte solution containing red cells. The lumen was perfused with a solution containing 14C-PEG. Net fluxes of water and ions were calculated during three 20-minute periods: basal, drug infusion, and recovery. Perfusion pressure was recorded to document changes in vascular resistance. Agents infused included the nitric oxide synthase substrate L-arginine, the nitric oxide source sodium nitroprusside, the substrate control D-arginine, and the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester.
  
  
  RESULTS
  L-arginine and sodium nitroprusside caused absorption of water and ions. NG-nitro-L-arginine methyl ester caused secretion of water and ions, which was prevented by synchronous infusion of L-arginine. Infusion of D-arginine had no effect. Both L-arginine and sodium nitroprusside caused mild vasodilation.
  
  
  CONCLUSIONS
  Inhibition of endogenous nitric oxide synthesis by NG-nitro-L-arginine methyl ester causes secretion of water and ions. This secretion is reversed by administration of the nitric oxide synthase substrate L-arginine. These findings are consistent with the hypothesis that endogenous nitric oxide has a proabsorptive influence over the ileum in the basal state.
authors.affiliation: Department of Surgery, Johns Hopkins Medical Institutions, Baltimore, Maryland.
authors.forename: M K
authors.initials: MK
authors.lastname: Barry
authors.forename: J D
authors.initials: JD
authors.lastname: Aloisi
authors.forename: S P
authors.initials: SP
authors.lastname: Pickering
authors.forename: C J
authors.initials: CJ
authors.lastname: Yeo
mesh_terms: D000042:Absorption; D000818:Animals; D001692:Biological Transport; D001834:Body Water / Q000378:metabolism*; D004573:Electrolytes / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism*; D009569:Nitric Oxide / Q000502:physiology*; D010477:Perfusion; D011817:Rabbits
chemical_list: D004573:Electrolytes; D009569:Nitric Oxide