Scavenging action of propolis extract against oxygen radicals.
The ethanolic extracts of two types of cuban propolis (R and P) showed a similar manner of scavenging action against different species of oxygen radicals which were generated by specific chemical reactions. Chemiluminescence produced by superoxide generated from the xanthine-xanthine oxidase reaction was 50% inhibited by approximately 5 micrograms/ml of propolis R and 9.5 micrograms/ml of propolis P and by catechin (0.15 micrograms/ml) and superoxide dismutase (72 ng/ml). Alkoxy radical scavenging effect was similar to that produced by 0.11 micrograms/ml of alpha-tocopherol: inhibition of chemiluminescence by 50% was caused by approximately 0.6 micrograms/ml of both propolis preparations. The results indicate that the antioxidative properties of both propolis could be attributed to their free radical scavenging activity against alkoxy radicals and to a lesser degree against superoxide.
Pascual
C
C
National Center for Scientific Research, Clinical Biochemistry Department, Playa, La Habana, Cuba.
Gonzalez
R
R
Torricella
R G
RG
D002392:Catechin / Q000494:pharmacology; D004347:Drug Interactions; D016166:Free Radical Scavengers; D008163:Luminescent Measurements; D011429:Propolis / Q000302:isolation & purification / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D016166:Free Radical Scavengers; D014970:Xanthines; D002392:Catechin; D011429:Propolis; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase