Molecular analysis of transgenic plants generated by microprojectile bombardment: effect of petunia transformation booster sequence.
Supercoiled plasmid expression vectors containing the petunia transformation booster sequence (TBS) were introduced by microprojectile bombardment into dicotyledenous (tobacco) and monocotyledonous (maize) cells. TBS effected a 7.8- to 16-fold increase in transformation frequencies in tobacco, and a 1.7- to 2.4-fold increase in maize. Although TBS contains a well-defined transcription enhancer element, no increases in plasmid gene expression were observed. TBS did not alter integration patterns in transformants, and did not affect segregation of linkage in R1 progeny. Computer analyses of the TBS sequence revealed numerous modular elements previously shown to be associated with putative chromosomal replication origin regions in eukaryotes, including DNA unwinding elements, scaffold-associated regions and pyrimidine tracts.
Buising
C M
CM
Department of Zoology and Genetics, Iowa State University, Ames 50011-3223.
Benbow
R M
RM
D000613:Aminobutyrates / Q000494:pharmacology; D000704:Analysis of Variance; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D004351:Drug Resistance / Q000235:genetics; D015870:Gene Expression; D006540:Herbicides / Q000494:pharmacology; D007613:Kanamycin Resistance / Q000235:genetics; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics*; D010947:Plants, Toxic; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics
D000613:Aminobutyrates; D006540:Herbicides; C003121:phosphinothricin