Immunocytochemical localization of choline-phospholipids in postnatal mouse molars.
This localization was studied using anti-choline phospholipid monoclonal IgM antibody (MC22-33F). By immunofluorescence, the apical portions of enamel-secreting ameloblasts and dentine-secreting odontoblasts were positively stained with MC22-33F. By immunoelectron microscopy, lysosomes in preodontoblasts, odontoblasts, preameloblasts and ameloblasts as well as matrix vesicles in dentine were strongly stained with MC22-33F. On the other hand, plasma membrane of these cells did not stain with MC22-33F. The frequency of positively reacting lysosomes in ameloblasts and odontoblasts and of matrix vesicles increased with cell maturation and mineral deposition.
Tsuji
T
T
Department of Oral Pathology, Okayama University Dental School, Japan.
Mark
M P
MP
Ruch
J V
JV
D000565:Ameloblasts / Q000378:metabolism; D000818:Animals; D007150:Immunohistochemistry; D051379:Mice; D008963:Molar; D009804:Odontoblasts / Q000378:metabolism; D009805:Odontogenesis; D010713:Phosphatidylcholines / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D014070:Tooth / Q000378:metabolism* / Q000648:ultrastructure
D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C026508:phosphatidyldimethylethanolamine