Regulation of human immunodeficiency virus Nef protein by phosphorylation.
Human immunodeficiency virus isolates express a Nef protein with either an alanine or a threonine at amino acid residue 15. The threonine residue is a site for phosphorylation by protein kinase C. Jurkat T cells constitutively expressing the alanine variant of Nef exhibit the ability to downregulate the induction of transcription factors NF-kB and AP-1. In contrast, Jurkat cells with the threonine variant of Nef are at least partially restored in their ability to recruit NF-kB and AP-1.
Bandres
J C
JC
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri.
Luria
S
S
Ratner
L
L
D001665:Binding Sites; D004279:DNA, Viral / Q000378:metabolism; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015693:Gene Products, nef / Q000032:analysis / Q000737:chemistry* / Q000502:physiology*; D015754:Genes, nef / Q000235:genetics; D006678:HIV / Q000737:chemistry / Q000378:metabolism*; D016325:HIV Long Terminal Repeat / Q000235:genetics; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics; D016328:NF-kappa B / Q000096:biosynthesis*; D010766:Phosphorylation; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D013912:Threonine / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D054311:nef Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015693:Gene Products, nef; D007376:Interleukin-2; D016328:NF-kappa B; D016755:Proto-Oncogene Proteins c-jun; D054311:nef Gene Products, Human Immunodeficiency Virus; D013912:Threonine