title: Inhibition of growth hormone bioactivity by recombinant human growth hormone-binding protein in the eluted stain assay system.
abstract: The effects of a recombinant human GH-binding protein (rhGHBP; amino acids 1-238) on GH stimulation of rat Nb2 lymphoma cells were examined with an eluted stain assay system (ESTA). This precise bioassay utilizes the colorimetric reduction by stimulated Nb2 cells of a yellow tetrazolium salt (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) to a purple formazan as its end-point. The use of a lactogenic bioassay allowed the investigation of hGHBP specificity for human GH (hGH) as opposed to prolactin. rhGHBP inhibited pituitary hGH bioactivity in a dose-dependent manner. No significant inhibition of prolactin or ACTH bioactivity occurred. It was confirmed that recombinant 20 kDa hGH also stimulated the Nb2 cells and that its relative potency was approximately 10% of that of pituitary-derived hGH. Stimulation by 20 kDa hGH was also inhibited by rhGHBP. The highly quantitative ESTA system demonstrated that the binding protein inhibited in a competitive manner. hGH activation of the Nb2 cells did not appear to be governed by a Michaelian first-order reaction. As might then be anticipated, the concentration of rhGHBP required for 50% inhibition of GH bioactivity (IC50) changed with agonist concentrations for both 20 kDa and 22 kDa hGH. However, with equimolar concentrations of these two isohormones, the IC50 of the binding protein was virtually identical. Potentiation of hGH bioactivity in vivo by low concentrations of hGHBP has been reported but was not observed in our in vitro system when tested over a wide range of binding protein concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Chemical Pathology, Middlesex Hospital, London, UK.
authors.forename: M T
authors.initials: MT
authors.lastname: Dattani
authors.forename: P C
authors.initials: PC
authors.lastname: Hindmarsh
authors.forename: C G
authors.initials: CG
authors.lastname: Brook
authors.forename: I C
authors.initials: IC
authors.lastname: Robinson
authors.forename: N J
authors.initials: NJ
authors.lastname: Marshall
mesh_terms: D000818:Animals; D001681:Biological Assay; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D003124:Colorimetry; D013006:Growth Hormone / Q000378:metabolism*; D006801:Humans; D008223:Lymphoma / Q000473:pathology; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; D013006:Growth Hormone; C051815:somatotropin-binding protein