Association of class II sequences encoding DR1 and DQ5 specificities with hypersensitivity to chironomid allergen Chi t I.
A panel of 188 unrelated Caucasian subjects who were exposed to the larvae of Chironomus thummi (Diptera, nonbiting midges) was HLA-typed by polymerase chain reaction amplification of the second exons of the DRB, DQA1, and DQB1 genes followed by dot-blot hybridization with sequence-specific oligonucleotide probes. Type I sensitization to the allergen Chi t I and a large number of other inhalant allergens was determined by RAST and skin testing. Sixty-one individuals were found to be sensitized to Chi t I, of whom 24 were sensitive to this allergen and to no other allergens tested. Statistical analyses showed that only in the latter group were the HLA-D genes DRB1*0101, DQA1*0101, and DQB1*0501 associated with IgE-responsiveness to Chi t I. These results suggest that HLA associations with responsiveness to certain allergens may be more striking in monosensitized subjects.
Tautz
C
C
Professional Research Institute for Occupational Medicine, Ruhr-Universität, Bochum, Germany.
Rihs
H P
HP
Thiele
A
A
Zwollo
P
P
Freidhoff
L R
LR
Marsh
D G
DG
Baur
X
X
D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology*; D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000276:immunology*; D005260:Female; D005815:Genetic Code / Q000235:genetics / Q000276:immunology*; D006681:HLA-D Antigens / Q000235:genetics / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D015798:HLA-DR1 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000453:epidemiology / Q000235:genetics / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007814:Larva / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D011110:Polymorphism, Genetic / Q000235:genetics / Q000276:immunology*; D012044:Regression Analysis; D017422:Sequence Analysis, DNA / Q000706:statistics & numerical data; D012882:Skin Tests
D000485:Allergens; D006681:HLA-D Antigens; D006683:HLA-DQ Antigens; D015798:HLA-DR1 Antigen; D007073:Immunoglobulin E