title: Nuf2, a spindle pole body-associated protein required for nuclear division in yeast.
abstract: The NUF2 gene of the yeast Saccharomyces cerevisiae encodes an essential 53-kd protein with a high content of potential coiled-coil structure similar to myosin. Nuf2 is associated with the spindle pole body (SPB) as determined by coimmunofluorescence with known SPB proteins. Nuf2 appears to be localized to the intranuclear region and is a candidate for a protein involved in SPB separation. The nuclear association of Nuf2 can be disrupted, in part, by 1 M salt but not by the detergent Triton X-100. All Nuf2 can be removed from nuclei by 8 M urea extraction. In this regard, Nuf2 is similar to other SPB-associated proteins including Nufl/SPC110, also a coiled-coil protein. Temperature-sensitive alleles of NUF2 were generated within the coiled-coil region of Nuf2 and such NUF2 mutant cells rapidly arrest after temperature shift with a single undivided or partially divided nucleus in the bud neck, a shortened mitotic spindle and their DNA fully replicated. In sum, Nuf2 is a protein associated with the SPB that is critical for nuclear division. Anti-Nuf2 antibodies also recognize a mammalian 73-kd protein and display centrosome staining of mammalian tissue culture cells suggesting the presence of a protein with similar function.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544-1014.
authors.forename: M A
authors.initials: MA
authors.lastname: Osborne
authors.forename: G
authors.initials: G
authors.lastname: Schlenstedt
authors.forename: T
authors.initials: T
authors.lastname: Jinks
authors.forename: P A
authors.initials: PA
authors.lastname: Silver
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle; D002467:Cell Nucleus / Q000502:physiology*; D004271:DNA, Fungal; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D008941:Spindle Apparatus / Q000378:metabolism*; D013696:Temperature
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; C087340:NUF2 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins