title: [Effect of the time of sample collection after exertion and the specimen handling on the lactate content in the blood of horses].
abstract: The mean maximal lactate concentration of horses after exercise (Lamax) amounted to 4.5 +/- 2.2 mmol/l for gallop workouts of less than 11 m/s and more than 180 s (n = 35; category A), at more than 11 m/s and less than 180 s duration (n = 17; category B) to 16.3 +/- 4.8 mmol/l and at less than 11 m/s and less than 180 s duration (n = 10; category C) to 4.6 +/- 1.6 mmol/l. Lamax was always measured immediately after exercise following exercise of categories A and C. But for exercise bouts of category B Lamax was measured between the first and the tenth minute after exercise. Following exercise bouts in this category, mean lactate concentrations measured in two-minute intervals before and after Lamax always differed significantly (p < 0.01 to p < 0.001). The lactate concentration did not vary within seven days in whole blood samples deproteinized immediately after collection and in plasma samples. In the same sample material, when kept frozen, lactate contents remained stable for 60 days. In whole blood samples treated with Na-heparin or Na-heparin/Na-fluoride stored at 20-22 degrees C and 4 degrees C, the lactate concentration had already increased significantly within the first 24 hours.
authors.affiliation: Institut für Anatomie, Physiologie und Hygiene der Haustiere der Rheinischen Friedrich-Wilhelms-Universität, Bonn.
authors.forename: A
authors.initials: A
authors.lastname: Lindner
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000662:veterinary; D001800:Blood Specimen Collection / Q000662:veterinary*; D006493:Heparin; D006736:Horses / Q000097:blood*; D007773:Lactates / Q000097:blood*; D005082:Physical Exertion / Q000502:physiology*; D012016:Reference Values; D013048:Specimen Handling / Q000662:veterinary*; D013696:Temperature; D013997:Time Factors
chemical_list: D007773:Lactates; D006493:Heparin
vernacular_title: Einfluss des Zeitpunktes der Probengewinnung nach Belastung und der Probenbehandlung auf die Laktatgehalte im Blut von Pferden.