Changes in synovial fluid N-acetyl-beta-glucosaminidase activity in the human temporomandibular joint with dysfunction.
beta-Glycosidases (N-acetyl-beta-glucosaminidase, N-acetyl-beta-galactosaminidase, beta-glucuronidase) were assayed in temporomandibular joint (TMJ) synovial fluid obtained from 23 patients with closed lock TMJ internal derangement (ID), four with closed-lock TMJ osteoarthritis (OA), and 13 with normal controls (N). Synovial fluid was collected from the upper joint space after injecting 1.5 ml of 1% lidocaine three times. The specific activity of N-acetyl-beta-glucosaminidase increased significantly both with ID (p < 0.01) and with OA (p < 0.001), along with increases in the activity of N-acetyl-beta-galactosaminidase (p < 0.05 with ID and p < 0.01 with OA) and in beta-glucuronidase (p < 0.05 both with ID and OA). The N-acetyl-beta-glucosaminidase activity with OA was also significantly higher (p < 0.001) than with ID. These findings suggest that N-acetyl-beta-glucosaminidase activity in the TMJ synovial fluid reflects the degree of TMJ dysfunction.
Kamada
A
A
Department of Biochemistry, Osaka Dental University, Japan.
Fujita
A
A
Kakudo
K
K
Okazaki
J
J
Ida
M
M
Sakaki
T
T
D000118:Acetylglucosaminidase / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology; D010865:Pilot Projects; D013582:Synovial Fluid / Q000201:enzymology*; D013705:Temporomandibular Joint Disorders / Q000201:enzymology*; D001617:beta-Glucosidase / Q000378:metabolism*
D006820:Hyaluronic Acid; D001617:beta-Glucosidase; D000118:Acetylglucosaminidase