Coating of liposomes with transferrin: physicochemical study of the transferrin-lipid system.
Transferrin was incorporated into the external surface of liposomes that were previously derivatized with ceramides. This new hydrophobic derivative of transferrin was incubated with liposomes at different protein-to-lipid ratios. The surface activity of both native and derivatized transferrin was determined with monomolecular layers as a membrane model. The maximal interaction was found with dipalmitoylphosphatidylcholine in all the experiences.
Egea
M A
MA
Physicochemical Unit, Faculty of Pharmacy, University of Barcelona, Spain.
García
M L
ML
Alsina
M A
MA
Reig
F
F
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002518:Ceramides / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007700:Kinetics; D008081:Liposomes; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D012545:Schiff Bases / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D013816:Thermodynamics; D014168:Transferrin / Q000008:administration & dosage* / Q000737:chemistry
D002518:Ceramides; D008081:Liposomes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D012545:Schiff Bases; D014168:Transferrin; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C017251:1,2-oleoylphosphatidylcholine