Characterization of cloned aortic smooth muscle cells from young rats.
Clones were derived by dilute plating from cultured aortic smooth muscle cells of 12-day-old rats. Clones Pup I to III resemble uncloned smooth muscle cultures from aortas of rat pups and from adult rat neointimas. They have a cobblestone morphology and proliferate in plasma-derived serum. By Northern analysis they contain platelet-derived growth factor B chain (PDGF-B) mRNA and high levels of CYPIA1, elastin, and osteopontin mRNAs, and they lack platelet-derived growth factor alpha-receptor (PDGF-alpha R) mRNA. In contrast, Pup V resembles smooth muscle cultures derived from uninjured adult rat arteries. It has an elongated morphology and proliferates poorly in plasma-derived serum. This clone expresses PDGF-alpha R mRNA, low levels of elastin and osteopontin mRNAs, and lacks CYPIA1 and PDGF-B mRNAs. Pup IV and VI have most of the properties of Pup I to III. We conclude that uncloned pup cultures are heterogeneous, but that the growth properties and gene expression pattern described for the uncloned culture is characteristic of individual clones within the population.
Lemire
J M
JM
Department of Pathology, University of Washington School of Medicine, Seattle 98195.
Covin
C W
CW
White
S
S
Giachelli
C M
CM
Schwartz
S M
SM
D000199:Actins / Q000032:analysis; D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000166:cytology / Q000293:injuries; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D002999:Clone Cells; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004549:Elastin / Q000235:genetics; D005786:Gene Expression Regulation; D008297:Male; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000166:cytology* / Q000293:injuries; D009218:Myosins / Q000032:analysis; D053495:Osteopontin; D010088:Oxidoreductases / Q000235:genetics; D010641:Phenotype; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000235:genetics
D000199:Actins; D010982:Platelet-Derived Growth Factor; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D004549:Elastin; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1; D009218:Myosins