title: Developmental stages in experimental liver metastases: relation to invasiveness.
abstract: We have previously reported that an invasive morphotype can be evoked in a rat colon carcinoma by transplanting it into pre-induced subcutaneous granulation tissue. We have now studied the interaction of the same tumor with liver tissue, which is extremely poor in connective tissue in comparison with the subcutaneous site. Tumor cells were injected into the portal system and the resulting experimental liver metastases were examined by electron microscopy and immunohistochemistry. Early metastases consisted of well-differentiated acini, fully surrounded by connective tissue that was derived from the periportal stroma. In a later stage, this connective tissue was overgrown by tumor cells and, almost immediately, acinar differentiation was lost. Most metastases eventually reached the liver capsule, which reacted by forming a layer of granulation tissue. Only in this layer, we observed invasion by thin tumor cell strands, which were often intimately associated with fibroblasts or with blood capillaries. The tumor cells remained smooth and rounded during this process. After fully penetrating the granulation tissue, the tumor cell strands reached the liver surface, where they formed poorly structured papillary masses that were nearly devoid of stroma. Our observations indicate that, even in a relatively homogeneous organ like the liver, the tumor-host interaction is highly complex and dynamic. They also confirm the notion that granulation tissue stimulates tumor invasiveness. Finally, they show that tumor cells can actively invade host tissues without exhibiting a "fibroblastic" morphology.
authors.affiliation: Department of Pathology, University of Amsterdam, The Netherlands.
authors.forename: K P
authors.initials: KP
authors.lastname: Dingemans
authors.forename: M A
authors.initials: MA
authors.lastname: van den Bergh Weerman
authors.forename: R F
authors.initials: RF
authors.lastname: Keep
authors.forename: P K
authors.initials: PK
authors.lastname: Das
mesh_terms: D000818:Animals; D002455:Cell Division; D003110:Colonic Neoplasms / Q000473:pathology*; D003238:Connective Tissue / Q000473:pathology; D008099:Liver / Q000473:pathology*; D008113:Liver Neoplasms / Q000556:secondary*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D051381:Rats