Basal metallothionein in tumors: widespread presence of apoprotein.
A survey has been conducted of solid and ascites tumors from mice and solid tumors in rats for the presence of metallothionein or metallothionein-like protein. In most tumors, a positive identification was made on the basis of Sephadex G-75 and HPLC-DEAE chromatography followed by competitive radioimmunoassay for metallothionein. Apometallothionein was revealed in a number of tumors for the first time by comparing the Sephadex G-75 chromatographic profiles of Zn in native cytosol and Cd in cytosol incubated briefly with CdCl2 to saturate free binding sites on the protein before Sephadex G-75 chromatography. In two cases unsaturation of metallothionein was correlated with a lack of zinc in the ascites fluid which supplies the tumor with zinc.
Pattanaik
A
A
Department of Chemistry, University of Wisconsin-Milwaukee 53201.
Shaw
C F
CF
Petering
D H
DH
Garvey
J
J
Kraker
A J
AJ
D000818:Animals; D001059:Apoproteins / Q000032:analysis*; D001667:Binding, Competitive; D002104:Cadmium / Q000032:analysis / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry; D008546:Melanoma, Experimental / Q000737:chemistry; D008668:Metallothionein / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000737:chemistry*; D011863:Radioimmunoassay; D051381:Rats; D012208:Rhabdomyosarcoma / Q000737:chemistry; D012513:Sarcoma, Experimental / Q000737:chemistry; D013160:Splenic Neoplasms / Q000737:chemistry; D015032:Zinc / Q000032:analysis / Q000378:metabolism
D001059:Apoproteins; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc