title: Virus DNA detection of herpes simplex keratitis by PCR.
abstract: HSV-DNA of seven corneal lesions suspected with herpes simplex keratitis (HSK) and nine normal human donor corneas were detected by PCR. Five out of seven diseased corneas showed positive results, and the other two diseased corneas and nine normal corneas negative. The results suggest the PCR may be useful as a rapid and sensitive method for diagnosing HSK.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-Sen University of Medical Sciences, Guangzhou, China.
authors.forename: K
authors.initials: K
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Zhuo
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000328:Adult; D001483:Base Sequence; D003315:Cornea / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007635:Keratitis, Dendritic / Q000382:microbiology; D016849:Keratitis, Herpetic / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral