Interleukin-6-induced tyrosine phosphorylation of multiple proteins in murine hematopoietic lineage cells.
Interleukin-6 (IL-6) is a multifunctional cytokine playing various roles in the immune system, hematopoiesis and acute phase reactions. To elucidate the intracellular signal transduction mechanism through the IL-6 receptor, we investigated IL-6-induced protein tyrosine phosphorylation in murine hematopoietic cell lines, BAFm130 and Y6. IL-6 stimulated tyrosine phosphorylation of multiple cellular proteins, such as gp130, an IL-6 signal transducer; Jak family of the cytoplasmic tyrosine kinases; and the latent cytoplasmic signal transducer and activator of transcription. We showed that the pattern of these tyrosine-phosphorylated molecules was different between BAFm130 and Y6 cells on which IL-6 exhibited different biological activities.
Matsuda
T
T
Biomedical Research Center, Osaka University Medical School, Japan.
Yamanaka
Y
Y
Hirano
T
T
D000818:Animals; D015703:Antigens, CD; D002460:Cell Line; D050822:Cytokine Receptor gp130; D006413:Hematopoietic System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015850:Interleukin-6 / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000187:drug effects / Q000378:metabolism; D019947:Receptors, Interleukin-6; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism*
D015703:Antigens, CD; C494200:Il6st protein, mouse; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D011506:Proteins; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases