title: Identification and characterisation of a novel repetitive antigen from Onchocerca spp.
abstract: A novel repetitive antigen from the cattle parasite Onchocerca gibsoni was shown to be recognised by sera from humans infected with Onchocerca volvulus, Wuchereria bancroftii or Brugia malayi. The O. gibsoni protein was produced in a recombinant form, and antibodies raised to this protein used to screen cDNA libraries for O. volvulus. A series of clones were isolated which encoded repetitive regions very similar to those in O. gibsoni, but interspersed between these were longer repeating units which we have not so far found in O. gibsoni. The repetitive antigen was shown to be of high molecular weight and present only in the insoluble (membrane) fraction of O. gibsoni microfilariae. Immunofluorescence techniques demonstrated that the antigen was associated both with muscle and with specific membrane layers, including a peripheral layer which corresponds to either the outer hypodermis or an inner region of the cuticle in adult female O. gibsoni. In many respects, the proteins encoded by the O. gibsoni and O. volvulus cDNA clones resembled repetitive antigens from several distantly related eukaryotic parasites, and a possible common role in immune evasion is discussed.
authors.affiliation: Department of Chemistry and Biochemistry, James Cook University of North Queensland, Townsville, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Catmull
authors.forename: D
authors.initials: D
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Ruggiero
authors.forename: D B
authors.initials: DB
authors.lastname: Copeman
authors.forename: D J
authors.initials: DJ
authors.lastname: Miller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017178:Brugia malayi / Q000276:immunology; D003429:Cross Reactions; D018076:DNA, Complementary / Q000235:genetics; D004605:Elephantiasis, Filarial / Q000276:immunology; D005260:Female; D005368:Filariasis / Q000276:immunology; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008842:Microfilariae / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D009854:Onchocerca / Q000235:genetics / Q000276:immunology*; D017181:Onchocerca volvulus / Q000235:genetics / Q000276:immunology; D009855:Onchocerciasis / Q000276:immunology / Q000469:parasitology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D014958:Wuchereria bancrofti / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D015801:Helminth Proteins; D011994:Recombinant Proteins