title: Structures of small oligomers liberated from barley arabinoxylans by endoxylanase from Aspergillus awamori.
abstract: Arabinoxylans consisting of more than 90% of arabinose and xylose were extracted from water-insoluble cell wall material of dehusked barley grain, and degraded with a purified endo-(1-->4)-beta-D-xylanase from Aspergillus awamori. Twelve of the oligosaccharide fragments released were isolated by a combination of size-exclusion and anion-exchange chromatography, and their structures were determined by 1H NMR spectroscopy. The oligosaccharides identified consisted of (1-->4)-linked beta-D-xylopyranosyl residues, some of which were substituted at O-2, at O-3, or at both O-2 and O-3, with alpha-L-arabinofuranosyl groups. Two new structures, a tetrasaccharide and a pentasaccharide, both containing terminal 2-O-arabinofuranosylxylopyranose as a structural element are described.
authors.affiliation: Department of Food Science, Agricultural University, Wageningen, Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Viëtor
authors.forename: R A
authors.initials: RA
authors.lastname: Hoffmann
authors.forename: S A
authors.initials: SA
authors.lastname: Angelino
authors.forename: A G
authors.initials: AG
authors.lastname: Voragen
authors.forename: J P
authors.initials: JP
authors.lastname: Kamerling
authors.forename: J F
authors.initials: JF
authors.lastname: Vliegenthart
mesh_terms: D001089:Arabinose / Q000032:analysis; D001230:Aspergillus / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D043364:Endo-1,4-beta Xylanases; D006026:Glycoside Hydrolases / Q000378:metabolism*; D001467:Hordeum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification; D014994:Xylose / Q000032:analysis
chemical_list: D009844:Oligosaccharides; D014990:Xylans; C085118:arabinoxylan; D014994:Xylose; D001089:Arabinose; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases