Increased expression of c-met messenger RNA following acute gastric injury in rats.
The aim of the present study was to evaluate messenger RNA expression of c-met, a hepatocyte growth factor receptor gene, after gastric mucosal injury in rats. Male Sprague-Dawley rats were fasted for 24 hours, received 0.6 N hydrochloric acid (HCl), and served for polyadenylated RNA extraction from the oxyntic gastric mucosa. The transcripts of rat c-met gene were analyzed by reverse-transcript polymerase chain reaction and Northern blotting. Although it was detected even before the HCl administration, the c-met expression increased 6, 24 and 48 hours after the HCl administration. Thereafter, gastric mucosal injury diminished and the c-met expression declined. Hepatocyte growth factor reportedly plays an important role in gastric cell proliferation. The increased c-met expression indicates that this gene may participate in the healing process of gastric mucosa after injury.
Tsujii
M
M
First Department of Medicine, Osaka University School of Medicine, Suita, Japan.
Kawano
S
S
Tsuji
S
S
Ito
T
T
Hayashi
N
N
Horimoto
M
M
Mita
E
E
Nagano
K
K
Masuda
E
E
Hayashi
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006851:Hydrochloric Acid / Q000633:toxicity; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019859:Proto-Oncogene Proteins c-met; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met; D020794:Receptor Protein-Tyrosine Kinases; D006851:Hydrochloric Acid