High-affinity cytotoxic T lymphocytes after non-HLA-sharing blood transfusion--the other side of the coin.
Previously, we have shown that pretransplantation blood transfusion modulates the T cell repertoire to a great extent. Patients receiving a BT from a donor sharing one HLA haplotype with the patient (HLA-sharing BT) develop CTL nonresponsiveness against cells of the BT donor and show a selective decrease in the usage of T cell receptor V beta families. The present study has focused on the analysis of the T cell repertoire in patients receiving an HLA mismatched (non-HLA-sharing) BT. CTL precursor frequencies were measured against single class I-mismatched antigens in split-well analysis. In addition, blocking studies of CTL-target cell interaction were performed with anti-CD8 monoclonal antibodies. The results demonstrate that non-HLA-sharing BT immunizes and induces the generation of CD8 independent, high-affinity CTL against immunogenic class I-mismatched antigens. Such HLA class I antigens might become nonacceptable mismatches in subsequent organ transplantation.
van Twuyver
E
E
Dept. of Transplantation Immunology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Mooijaart
R J
RJ
ten Berge
I J
IJ
van der Horst
A R
AR
Wilmink
J M
JM
Claas
F H
FH
de Waal
L P
LP
D000483:Alleles; D000911:Antibodies, Monoclonal / Q000276:immunology; D001787:Blood Group Incompatibility / Q000209:etiology; D016827:CD8 Antigens / Q000276:immunology; D015536:Down-Regulation; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000502:physiology; D065227:Transfusion Reaction
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D006680:HLA Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta