Functional regions of ornithine decarboxylase antizyme.
Degradation of ornithine decarboxylase, a key enzyme in polyamine biosynthesis, is accelerated by the binding of antizyme, an ornithine decarboxylase inhibitory protein induced by polyamines. In the present study, we examined the effects of a series of deletion mutants of rat antizyme. The results indicated that two regions of antizyme, one internal (amino acids 122-144) and the other near the C-terminus (amino acids 211-218) are necessary for its binding to ornithine decarboxylase and inhibition of its activity, and an additional internal region (amino acids 88-118, especially 113-118) is necessary for its destabilization.
Ichiba
T
T
Department of Nutrition, Jikei University School of Medicine, Tokyo, Japan.
Matsufuji
S
S
Miyazaki
Y
Y
Murakami
Y
Y
Tanaka
K
K
Ichihara
A
A
Hayashi
S
S
D000818:Animals; D001665:Binding Sites; D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins / Q000737:chemistry*; D051381:Rats; D011993:Recombinant Fusion Proteins; D017384:Sequence Deletion; D013329:Structure-Activity Relationship
D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins; D011993:Recombinant Fusion Proteins; C031017:ornithine decarboxylase antizyme