title: Binding of the lipid peroxidation product 4-hydroxynonenal to human polymorphonuclear leukocytes.
abstract: 4-Hydroxynonenal (HNE) is produced during peroxidation of polyunsaturated fatty acids. It exerts a chemokinetic effect on human polymorphonuclear leukocytes (PMN). Investigations of this mechanism were performed. The results indicate that [3H]-HNE binding to PMN results both in non-specific bonds to the numerous SH groups of the cells and in binding to a saturable, reversible and specific HNE site. Scatchard analysis revealed that this is a single site with an apparent affinity constant of 319 nM and a density of 1.57 pmol (10(6))-1 cells. This specific binding site may be involved in the chemokinetic effect of HNE.
authors.affiliation: Department of Experimental Medicine and Oncology, University of Turin, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Curzio
authors.forename: C
authors.initials: C
authors.lastname: Ferretti
authors.forename: R J
authors.initials: RJ
authors.lastname: Stephens
authors.forename: H
authors.initials: H
authors.lastname: Esterbauer
authors.forename: M U
authors.initials: MU
authors.lastname: Dianzani
mesh_terms: D000447:Aldehydes / Q000097:blood*; D001665:Binding Sites / Q000502:physiology; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation / Q000502:physiology*; D015394:Molecular Structure; D009504:Neutrophils / Q000378:metabolism*; D011869:Radioligand Assay
chemical_list: D000447:Aldehydes; C027576:4-hydroxy-2-nonenal