Association of H-ras mutations with adenocarcinomas of the parotid gland.
We have examined 17 adenocarcinomas and 2 mixed tumors of the salivary glands for mutational activation of the oncogenes H-ras, K-ras and N-ras. The presence of mutations was determined by in vitro amplification of gene fragments spanning codons 12, 13 and 61 and the use of mutation-specific oligonucleotide hybridization. ras mutations were present in 3 of 13 adenocarcinomas (23%) of the parotid gland. The mutations were confirmed and characterized by means of cycle sequencing of polymerase chain reaction (PCR) fragments. In all 3 cases, the mutation was an A:T to G:C transition at the second position of codon 61 of the H-ras gene. This rather unusual ras mutation could provide a clue to identify one or more carcinogens involved in the pathogenesis of parotid cancer.
van Halteren
H K
HK
Department of Surgery, The Netherlands Cancer Institute, Amsterdam.
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D000230:Adenocarcinoma / Q000235:genetics*; D001483:Base Sequence; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010307:Parotid Neoplasms / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*