[Quantitative diagnosis and histopathologic grading of chondrosarcoma].
Quantitative image analysis of nuclei, DNA content measurement and histopathological examination in 44 cases of chondrosarcoma were carried out. 21 cases of low grade, 15 of intermediate- grade, 8 of high-grade chondrosarcoma and 10 cases of benign chondroma were included in this study. A total of 19 morphometric parameters were measured and analyzed. The site of tumor and the age of patients were taken for account as well. Each value was tested by stepwise discrimination classification, of which 8 were shown to be of importance in quantitative diagnosis and grading of chondrosarcoma. They are: polynuclear cell index, OSD, mitosis index, Dmin, nuclear area, nuclear volume, cellular density as well as the tumor site. The accuracy was 95.06%. Significant (P < 0.05) or highly significant differences (P < 0.01) were found between the circle shape factor, binuclear cell index, tumor site and the age of patients in benign chondroma and low-grade chondrosarcoma groups. The measurement of nuclear DNA contents in 49 cases showed that the higher the grade of the chondrosarcoma was, the higher were the value of I O D DNA index and superploidy. DNA index of benign chondroma was from 1.0087 to 1.3095, > 1.3095 of malignant chondrosarcoma, and > 1.59 of high-grade chondrosarcoma groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Li
J P
JP
Department of Orthopedics, People's Hospital, Beijing Medical University.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001859:Bone Neoplasms / Q000175:diagnosis / Q000473:pathology*; D002467:Cell Nucleus / Q000473:pathology; D002812:Chondroma / Q000175:diagnosis / Q000473:pathology*; D002813:Chondrosarcoma / Q000175:diagnosis / Q000473:pathology*; D004273:DNA, Neoplasm / Q000235:genetics; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D011123:Polyploidy
D004273:DNA, Neoplasm