Molecular cloning of human brain glutamate/aspartate transporter II.
Glutamate transporters are membrane-bound proteins which are localized in glial cells and/or pre-synaptic glutamatergic nerve endings and are essential for the removal and termination of action of synaptic glutamate. Several cDNAs encoding glutamate transporters have been isolated from mammalian tissues, including human cerebellum. Here, we screened cDNA libraries derived from human brain stem and cerebellum, and isolated a novel cDNA that encodes for a glutamate transporter. This cDNA predicts a protein which contains 565 amino acids and is homologous to a rat brain Na(+)-dependent glutamate/aspartate transporter. The new cDNA is expressed in brain and is structurally distinct from the previously reported human glutamate transporter cDNA.
Shashidharan
P
P
Department of Neurology, Mount Sinai School of Medicine, New York, NY 10029.
Wittenberg
I
I
Plaitakis
A
A
D000595:Amino Acid Sequence; D027322:Amino Acid Transport System X-AG; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002531:Cerebellum / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D027322:Amino Acid Transport System X-AG; D002352:Carrier Proteins; D018076:DNA, Complementary; D006023:Glycoproteins