[Preparation of digoxigenin labeled virG DNA probe of Shigella and its homology analysis].
Using hapten digoxigenin labeled 1. 4kb virG DNA probe of S. flexneri 2a YSH6000. We examined the DNA homology among 73 strains of Shigella, 32 strains of EIEC, 17 strains of Yersinia, 5 strains of S. typhi and 5 strains of E. coli. At the same time strains of Shigella, EIEC and Yesinia were tested by Sereny assay. We concluded that virG DNA is conserved both in Shigella and EIEC and is related to Sereny test only in Shigella and EIEC but not in Yesinia. The coincidence rate of hybridization and Sereny test was 87.5% and 90.9% for S. sonni and EIEC respectively.
She
F
F
Laboratory of Genetic Engineering, Fujian Medical College, Fuzhou.
Bao
Y
Y
D001426:Bacterial Proteins / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004076:Digoxigenin; D004755:Enterobacteriaceae / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D012760:Shigella / Q000235:genetics*
D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D004076:Digoxigenin