[The effect of macromolecular adhesion factors isolated from the blood serum and liver of mammals on the intensity of protein synthesis and the ATP content in hepatocytes in vitro].
The influence of the extra-low dozes of the macromolecular adhesive factors (MAF) on the protein synthesis and on the ATP contents in the rat hepatocytes on different models in vitro has been investigated. The protein synthesis intensity, the cell penetrability for labelled 3H-leucine and the intracell ATP contents in the experiments with the hepatocyte monolayer in control cells has been showed to be the same as those in the cells treated by MAF-1 and MAF-2. In the liver cells the protein synthesis intensity was depressed essentially under the action of MAF-1 and MAF-2 with respect to the control cells. The penetrability of the liver cells has increased in 50% of experiments after MAF-1 and MAF-2 treatment. The data obtained show the necessity of preservation of morphological structure of organs for the realization of the biological activity of appropriate MAF.
Iamskova
V P
VP
Nechaeva
N V
NV
Tumanova
N B
NB
Iurovitskiĭ
Iu G
IuG
Novikova
T E
TE
Fateeva
V I
VI
Gvazava
I G
IG
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000302:isolation & purification / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D007930:Leucine / Q000493:pharmacokinetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D046911:Macromolecular Substances; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium
D015815:Cell Adhesion Molecules; D046911:Macromolecular Substances; D011506:Proteins; D014316:Tritium; D000255:Adenosine Triphosphate; D007930:Leucine
Issledovanie vliianiia makromolekuliarnykh adgezionnykh faktorov, vydelennykh iz syvorotki krovi i pecheni mlekopitaiushchikh, na intensivnost' sinteza belka i soderzhaniia ATF v gepatotsitakh in vitro.