Induction of cytotoxic T lymphocytes from splenocytes after orthotopic penetrating keratoplasty in the rat.
We used a rat model of orthotopic penetrating keratoplasty to study splenic cell cytotoxicity in the host. DA (RT1avl) rats received grafts of Fischer (F344, RT1lv1) rat corneas. Cytotoxic T lymphocyte (CTL) activity was measured by the cell-mediated lymphocytotoxicity assay using 4/4R.M.-4 cells as target cells. CTL activity became detectable in vitro 2 weeks after grafting and peaked at 3 weeks. This simple method of quantifying CTL activity in the orthotopic penetrating keratoplasty model should provide a reliable tool for studying the CTL response in corneal transplantation.
Minamoto
A
A
Department of Ophthalmology, Hiroshima University School of Medicine, Japan.
Sakata
H
H
Tsumura
K
K
Yamamoto
M
M
Kusunoki
S
S
D000818:Animals; D002478:Cells, Cultured; D016510:Corneal Neovascularization / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D006084:Graft Rejection / Q000276:immunology; D015948:Keratoplasty, Penetrating / Q000276:immunology*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D016038:Skin Transplantation / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*