Simultaneous determination of (R)- and (S)-brefanolol in human plasma: high-performance liquid chromatographic assay on a chiral stationary phase.
A new method for the quantitative enantiospecific determination of rac-brefanolol, a vasodilating beta-adrenoceptor blocking agent, in human plasma is described. After an alkaline liquid-liquid extraction from biological material and a preseparation on a silica gel column with fractional collection of the eluate, the separation of the underivatized enantiomers is performed by high-performance liquid chromatography on cellulose tris-4-methylbenzoic acid polymer coated on silica gel. The UV-absorbance of the eluate was monitored at 254 nm. Determination limits are 10 ng enantiomer per ml plasma and allow the performance of pharmacokinetic studies after the aimed therapeutic dosages.
Neubeck
M
M
Pharmakologisches Institut für Naturwissenschaftler der Johann Wolfgang Goethe-Universität am Main, Germany.
Henke
D
D
Spahn-Langguth
H
H
Mutschler
E
E
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004983:Ethanolamines / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014665:Vasodilator Agents / Q000097:blood* / Q000493:pharmacokinetics
D004983:Ethanolamines; D014665:Vasodilator Agents; C065350:brefanolol