Persistent increases in dopamine D2 receptor mRNA expression in basal ganglia following kindling.
Amygdala kindling resulted in significant increases in the expression of D2 receptor mRNA in the nucleus accumbens and striatum 30 days following the last kindling stimulation. Densitometric analyses of tissue sections incubated in the presence of an oligonucleotide probe directed against D2 receptor cDNA indicated a 20-35% increase in D2 receptor mRNA in these regions following kindling. Kindling from the amygdala followed by piriform cortical kindling in the transfer paradigm (overkindling) resulted in significant further increases in D2 receptor mRNA expression in both the accumbens (150% increase) and striatum (120% increase). There were no observed hemispheric asymmetries in D2 receptor mRNA in either kindled or overkindled animals. The data indicate an enduring upregulation of extrapyramidal D2 receptor mRNA following the kindling process. How this change may relate to kindling-induced alterations in seizure susceptibility or behaviors mediated by limbic dopaminergic pathways are questions for future studies.
Gelbard
H A
HA
Department of Neurology, University of Rochester Medical Center, NY 14642.
Applegate
C D
CD
D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism*; D004558:Electric Stimulation; D017403:In Situ Hybridization; D007696:Kindling, Neurologic / Q000378:metabolism*; D008297:Male; D017072:Neostriatum / Q000378:metabolism; D009714:Nucleus Accumbens / Q000378:metabolism; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D017448:Receptors, Dopamine D2