title: Crystallization and preliminary X-ray crystallographic studies of recombinant human leukotriene A4 hydrolase complexed with bestatin.
abstract: Recombinant human leukotriene A4 hydrolase complexed with bestatin, an inhibitor of metalloprotease, has been crystallized by the hanging drop vapor diffusion method using 0.1 M phosphate buffer (pH 6.5) and 50 to 54% saturated ammonium sulfate. The orthorhombic crystals belong to the space group I222 or I2(1)2(1)2(1) with unit cell dimensions of a = 273.6 A, b = 261.3 A and c = 52.9 A. They diffract beyond 2.5 A resolution and a native data set up to 3 A resolution has been collected on an imaging plate Weissenberg camera using synchrotron radiation.
authors.affiliation: Life Science Research Laboratory, Japan Tobacco, Inc., Kanagawa.
authors.forename: H
authors.initials: H
authors.lastname: Tsuge
authors.forename: H
authors.initials: H
authors.lastname: Ago
authors.forename: M
authors.initials: M
authors.lastname: Aoki
authors.forename: M
authors.initials: M
authors.lastname: Furuno
authors.forename: M
authors.initials: M
authors.lastname: Noma
authors.forename: M
authors.initials: M
authors.lastname: Miyano
authors.forename: M
authors.initials: M
authors.lastname: Minami
authors.forename: T
authors.initials: T
authors.lastname: Izumi
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004851:Epoxide Hydrolases / Q000737:chemistry*; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives* / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011480:Protease Inhibitors / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D011480:Protease Inhibitors; D011994:Recombinant Proteins; D004851:Epoxide Hydrolases; D007930:Leucine; C012211:ubenimex; C043134:leukotriene A4 hydrolase