Differential expression of protein kinase C isozyme messenger RNAs in dunning R-3327 rat prostatic tumors.
Adenocarcinoma of the prostate is the second leading cause of cancer deaths in men. The protein kinase C (PKC) family of signal transducing kinases has been implicated in neoplastic transformation and progression in other tissues, and some evidence suggests roles for PKC in prostate growth and neoplasia. We have detected expression of eight specific PKC isozyme mRNAs (alpha, beta, gamma, delta, epsilon, eta, theta, and zeta) in normal rat whole prostate and found some of these to be differentially expressed in certain Dunning R-3327 rat prostatic adenocarcinoma sublines. PKC zeta mRNA was detected in normal prostate and Dunning H tumor, whereas an alternatively spliced form of PKC zeta RNA was found in Dunning G tumor and normal brain. Both forms of PKC zeta RNA were markedly reduced in the androgen insensitive, highly metastatic Dunning AT-3, MAT-Lu, and MAT-LyLu tumors. We have cloned and report the sequence of the novel portion of the alternatively spliced form of PKC zeta RNA, which is polyadenylated and present in cytoplasm.
Powell
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Urologic Oncology Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Fair
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Heston
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D000230:Adenocarcinoma / Q000201:enzymology*; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002455:Cell Division; D002460:Cell Line; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D007527:Isoenzymes / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000201:enzymology*; D011493:Protein Kinase C / Q000096:biosynthesis*; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D011944:Receptors, Androgen / Q000378:metabolism; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D007527:Isoenzymes; D015347:RNA Probes; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011493:Protein Kinase C