title: Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria.
abstract: Four human Lactobacillus acidophilus strains were tested for their ability to adhere onto human enterocyte like Caco-2 cells in culture. The LA 1 strain exhibited a high calcium independent adhesive property. This adhesion onto Caco-2 cells required a proteinaceous adhesion promoting factor, which was present in the spent bacterial broth culture supernatant. LA 1 strain also strongly bound to the mucus secreted by the homogeneous cultured human goblet cell line HT29-MTX. The inhibitory effect of LA 1 organisms against Caco-2 cell adhesion and cell invasion by a large variety of diarrhoeagenic bacteria was investigated. As a result, the following dose dependent inhibitions were obtained: (a) against the cell association of enterotoxigenic, diffusely adhering and enteropathogenic Escherichia coli, and Salmonella typhimurium; (b) against the cell invasion by enteropathogenic Escherichia coli, Yersinia pseudotuberculosis, and Salmonella typhimurium. Incubations of L acidophilus LA 1 before and together with enterovirulent E coli were more effective than incubation after infection by E coli.
authors.affiliation: Département de Microbiologie, UFR Sciences Pharmaceutiques, Paris, France.
authors.forename: M F
authors.initials: MF
authors.lastname: Bernet
authors.forename: D
authors.initials: D
authors.lastname: Brassart
authors.forename: J R
authors.initials: JR
authors.lastname: Neeser
authors.forename: A L
authors.initials: AL
authors.lastname: Servin
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001667:Binding, Competitive; D002460:Cell Line; D004755:Enterobacteriaceae / Q000502:physiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000648:ultrastructure; D007422:Intestines / Q000166:cytology / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning