title: Analysis of the role of 5-HT in the enteric nervous system using anti-idiotopic antibodies to 5-HT receptors.
abstract: The effects of anti-idiotypic antibodies (alpha-id) that recognize serotonin [5-hydroxytryptamine (5-HT)] receptors on myenteric neurons of the guinea pig small intestine were characterized electrophysiologically, and alpha-id binding sites were located immunocytochemically. Initial applications of the alpha-id mimicked each of three actions of 5-HT: a rapid depolarization, associated with a fall in input resistance (Rin), which was inhibited by the 5-HT3 antagonists tropisetron (> or = 1 microM) and renzapride (100 microM); a slow membrane depolarization, associated with increased Rin, that was inhibited by the 5-HT1P antagonist renzapride but was unaffected by a 5-HT4 blocking concentration of tropisetron (10 microM); and a hyperpolarization, associated with decreased Rin, that was antagonized by the 5-HT1A inhibitor NAN-190. Cross-desensitization was observed between responses to 5-HT and the alpha-id. After exposure to the alpha-id, subsequent responses to the alpha-id, 5-HT, and stimulus-evoked slow excitatory postsynaptic potentials were antagonized; however, responses to carbachol and substance P were unaffected. The alpha-id thus specifically inhibits the effects of endogenously released and exogenously applied 5-HT. The alpha-id bound to sites on myenteric and submucosal neurons and a subepithelial nerve plexus. Binding of the alpha-id was blocked by 5-HT1P-, 5-HT3-, and 5-HT4-specific antagonists. We concluded that the alpha-id binds selectively to all known subtypes of 5-HT receptor in the enteric nervous system and is thus useful for investigating the gastrointestinal function of 5-HT.
authors.affiliation: Department of Anatomy and Cell Biology, College of Physicians and Surgeons of Columbia University, New York, New York.
authors.forename: P R
authors.initials: PR
authors.lastname: Wade
authors.forename: H
authors.initials: H
authors.lastname: Tamir
authors.forename: A L
authors.initials: AL
authors.lastname: Kirchgessner
authors.forename: M D
authors.initials: MD
authors.lastname: Gershon
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology* / Q000494:pharmacology; D001666:Binding Sites, Antibody; D004594:Electrophysiology; D017615:Enteric Nervous System / Q000276:immunology / Q000502:physiology*; D006168:Guinea Pigs; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007150:Immunohistochemistry; D008297:Male; D011985:Receptors, Serotonin / Q000276:immunology*; D012701:Serotonin / Q000502:physiology*; D012702:Serotonin Antagonists / Q000494:pharmacology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D012701:Serotonin