title: A pyruvate decarboxylase gene from Aspergillus parasiticus.
abstract: A gene encoding a putative pyruvate decarboxylase (EC 4.1.1.1) was isolated from a genomic library of the filamentous fungus Aspergillus parasiticus strain SU-1. The deduced amino acid sequence showed 37% homology to PDC1 from Saccharomyces cerevisiae. Although A. parasiticus has an obligate growth requirement for oxygen, it produced ethanol in shake flask cultures indicating a response to anoxic conditions mediated by pyruvate decarboxylase.
authors.affiliation: Institute of Food Research, Norwich Research Park, Colney, UK.
authors.forename: V
authors.initials: V
authors.lastname: Sanchis
authors.forename: I
authors.initials: I
authors.lastname: Vinas
authors.forename: I N
authors.initials: IN
authors.lastname: Roberts
authors.forename: D J
authors.initials: DJ
authors.lastname: Jeenes
authors.forename: A J
authors.initials: AJ
authors.lastname: Watson
authors.forename: D B
authors.initials: DB
authors.lastname: Archer
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D000431:Ethanol / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011767:Pyruvate Decarboxylase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D000431:Ethanol; D011767:Pyruvate Decarboxylase