Cloning and sequencing of a cell surface protein-encoding gene conserved in sea urchin species.
We report the nucleotide sequence of a fragment of DNA derived from a sea urchin genomic clone containing the cell surface Bep4 (butanol-extracted protein 4)-encoding gene. The structural gene is interrupted by four introns and the promoter region contains TATA and CAAT consensus motifs. The transcription start point (tsp) was also determined. Remarkable homologies, between Bep4 and other proteins known to be involved in cell interactions, were observed regarding two potential Ca(2+)-binding sites and the corresponding DNA consensus sequences. We also report the conservation of the bep4 gene and its corresponding Bep4 protein between various sea urchin species by way of Southern and Western blotting.
Di Carlo
M
M
Istituto di Biologia dello Sviluppo, C.N.R., Palermo, Italy.
Perriera
S
S
Montana
G
G
Romancino
D P
DP
Reale
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D005796:Genes; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D012617:Sea Urchins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D016385:TATA Box
C065555:Bep4 protein, Paracentrotus lividus; D008565:Membrane Proteins