Plasmid-encoded catalase of Staphylococcus simulans biovar staphylolyticus.
Staphylococcus simulans biovar staphylolyticus contains five plasmids designated pACK1 through pACK5. Non-denaturing electrophoretic analysis of an extract prepared from wild-type cells revealed three bands of catalase activity, whereas an extract of cells cured of pACK1 produced only two catalase bands. Cloning and Southern hybridization analysis showed that there is a catalase structural gene on pACK1. The plasmid-specified catalase was the major activity produced under both aerobic and anaerobic conditions of growth.
Fondren
L M
LM
Department of Biological Sciences, University of Alabama, Tuscaloosa 35487.
Sloan
G L
GL
LeBlanc
P A
PA
Heath
H E
HE
D000332:Aerobiosis; D000693:Anaerobiosis; D002374:Catalase / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D013210:Staphylococcus / Q000145:classification / Q000201:enzymology / Q000235:genetics*
D007527:Isoenzymes; D002374:Catalase