Alkaline phosphodiesterase I release from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C--IV. The release from Cacia porcellus organs.
1. Alkaline phosphodiesterase I release from organs of Cacia porcellus by the action of phosphatidylinositol-specific phospholipase C (PIPLC) of Bacillus thuringiensis was studied. 2. A significant amount of alkaline phosphodiesterase I was released from both slices and homogenate of the kidney and small intestine but not from the liver or pancreas. 3. The release of the enzyme from kidney brush border membranes was dependent on, or proportional to, the reaction time and the PIPLC concentration. The enzyme release by PIPLC was suppressed when the PIPLC was heat-inactivated before addition to the reaction mixture. This suggests that the enzyme release must be due to direct action of PIPLC on kidney brush border membranes. 4. The released alkaline phosphodiesterase I had a molecular weight of 240,000 and was activated by Mg2+, but strongly inhibited by EDTA, thiol reagents and 5'-nucleotide-containing compounds.
Nakabayashi
T
T
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Matsuoka
Y
Y
Ikezawa
H
H
Kimura
Y
Y
D000818:Animals; D001413:Bacillus thuringiensis / Q000201:enzymology; D002462:Cell Membrane / Q000378:metabolism; D003902:Detergents; D005960:Glucosides; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D043264:Phosphodiesterase I; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D012995:Solubility; D013696:Temperature; D014781:Viscera / Q000378:metabolism*
D003902:Detergents; D005960:Glucosides; C018619:octyl-beta-D-glucoside; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase