title: Anatomy and dynamics of a ligand-binding pathway in myoglobin: the roles of residues 45, 60, 64, and 68.
abstract: In order for diatomic ligands to enter and exit myoglobin, there must be substantial displacements of amino acid side chains from their positions in the static X-ray structure. One pathway, involving Arg/Lys45, His64, and Val68, has been studied in greatest detail. In an earlier study (Lambright et al., 1989) we reported the surprising result that mutation of the surface residue Lys45 to arginine lowers the inner barrier to CO rebinding. Until then, it had been thought that this barrier primarily involves interior distal pocket residues such as His64 and Val68. In this report, we present a detailed study of the CO rebinding kinetics in aqueous solution of a series of single- and double-site mutants of human myoglobin at positions 64, 68, 45, and 60. On the basis of the observed kinetics, we propose that the effect of surface residue 45 on the inner barrier can be explained by a chain of interactions between surface and pocket residues. Very large, and in some cases unexpected, changes are observed in the kinetics of recombination and in the partitioning between geminate and bimolecular recombination.
authors.affiliation: Department of Chemistry, Stanford University, California 94305-5080.
authors.forename: D G
authors.initials: DG
authors.lastname: Lambright
authors.forename: S
authors.initials: S
authors.lastname: Balasubramanian
authors.forename: S M
authors.initials: SM
authors.lastname: Decatur
authors.forename: S G
authors.initials: SG
authors.lastname: Boxer
mesh_terms: D002248:Carbon Monoxide / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009154:Mutation; D009211:Myoglobin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013057:Spectrum Analysis; D013816:Thermodynamics
chemical_list: D008024:Ligands; D009211:Myoglobin; D011994:Recombinant Proteins; D002248:Carbon Monoxide