title: Can adenine nucleotides predict primary nonfunction of the human liver homograft?
abstract: Sixty-eight primary liver grafts were analyzed to see whether adenine nucleotides (AN: ATP, ADP, and AMP) or purine catabolites (PC: adenosine, inosine, hypoxanthine, and xanthine) of tissue or effluent can predict primary graft nonfunction. AN, PC, and nicotinamide adenine dinucleotide, oxidized form (NAD+) of the tissue before (pretransplant) and after graft reperfusion (post-transplant) and of the effluent were analyzed. The graft outcome was classified into two groups (group A: successful, n = 64; group B: primary nonfunctioning, n = 4). No significant differences were observed in pretransplant measurements between groups A and B, whereas ATP, ADP, total AN, total AN + total PC (T) and NAD+, in post-transplant tissues, were significantly higher in group A. Xanthine in the effluent was significantly higher in group B than in group A. ATP, ADP, total AN, T, and NAD+ in post-transplant tissue were significantly associated with primary graft nonfunction by logistic regression analysis.
authors.affiliation: Pittsburgh Transplantation Institute, University of Pittsburgh School of Medicine, PA 15213.
authors.forename: I
authors.initials: I
authors.lastname: Hamamoto
authors.forename: S
authors.initials: S
authors.lastname: Takaya
authors.forename: S
authors.initials: S
authors.lastname: Todo
authors.forename: O
authors.initials: O
authors.lastname: Bronsther
authors.forename: S
authors.initials: S
authors.lastname: Fujita
authors.forename: T M
authors.initials: TM
authors.lastname: Van Gulik
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: W
authors.initials: W
authors.lastname: Irish
authors.forename: T E
authors.initials: TE
authors.lastname: Starzl
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis* / Q000503:physiopathology; D006085:Graft Survival; D006801:Humans; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009243:NAD / Q000378:metabolism; D011687:Purines / Q000378:metabolism; D014184:Transplantation, Homologous
chemical_list: D000227:Adenine Nucleotides; D011687:Purines; D009243:NAD