[Prostaglandin E1 does not inhibit production of TNF and IL-1 by mononuclear cells after head-neck surgery].
We studied whether human blood mononuclear cells could produce tumor necrosis factor (TNF) and interleukin 1 (IL-1) by surgical stimuli, and if so, prostaglandin E1 (PGE1) could inhibit their increases before and following operation in patients undergoing head-neck surgery. The patients were randomly allocated into 2 groups; no PGE1 infusion group (control group, n = 6) and PGE1 infusion group (PGE1 group, n = 6). PGE1 group was infused intravenously with PGE1 at a rate of 30 ng.kg-1 x min-1 during surgery and at a rate of 10-30 ng.kg-1 x min-1 after surgery. Peripheral blood was withdrawn into heparinized syringes from these patients immediately before surgery and on the 1st postoperative day. Production of TNF on the 1st postoperative day increased 1.5 fold in control group and 1.4 fold in PGE1 group compared with those before surgery. Production of IL-1 on the 1st postoperative day increased 1.7 fold in control group and 1.1 fold in PGE1 group compared with those before surgery. Also, there were no significant differences between 2 groups in both productions of TNF and IL-1. These data indicate that human blood mononuclear cells would produce TNF and IL-1 by surgical stimuli and PGE1 could inhibit their production by surgical stimuli. It also provides evidence that PGE1 does not inhibit the body's defense response to surgical stimuli.
Enzan
K
K
Department of Emergency Medicine, Akita University School of Medicine.
Sato
W
W
Masaki
Y
Y
Kadosaki
M
M
Suzuki
M
M
D000368:Aged; D000527:Alprostadil / Q000494:pharmacology*; D006257:Head / Q000601:surgery; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008875:Middle Aged; D009333:Neck / Q000601:surgery; D013312:Stress, Physiological / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D000527:Alprostadil