Acrosome reaction in the head-attached human sperm.
The physiological acrosome reaction of the sperm occurs on the surface of the oocyte. Although its occurrence on the zona pellucida is considered to be a specific response to zona compounds, whether the simple attachment itself could influence the reaction of motile spermatozoa to a fixed surface and promote onset of the reaction is questioned. A motile human sperm head fixation method was developed recently, through which a group of head-attached sperm can be established easily. These head-attached sperm were investigated for their acrosome reactions. After staining by fluorescein iso-thiocyanate conjugated peanut lectin (FITC-PNA), many head-attached spermatozoa (75.7%) were found to have undergone an acrosome reaction.
Ou
M C
MC
Institute of Clinical Medicine, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
D000177:Acrosome / Q000502:physiology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013077:Sperm Head / Q000502:physiology*; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology