A chicken transferrin binding protein is heat shock protein 108.
A previously described transferrin binding protein from chicken oviduct has been identified as a heat shock protein, HSP108, by microsequencing of RP-HPLC purified tryptic peptides. The protein purified from oviduct by SDS-PAGE or from liver by ovotransferrin-affinity chromatography reacts with a monoclonal antibody raised against HSP108. The cDNA sequence predicts of KDEL peptide at the carboxyl terminus, but the protein does not react with anti-KDEL monoclonal antibodies, suggesting that HSP108 is processed in a way that eliminates the KDEL epitope.
Hayes
G R
GR
Department of Biochemistry and Molecular Biology, SUNY Health Science Center, Syracuse 13210.
Himpler
B S
BS
Weiner
K X
KX
Lucas
J J
JJ
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D033862:Iron-Binding Proteins; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D010057:Oviducts / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D033863:Transferrin-Binding Proteins
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D010446:Peptide Fragments; D033863:Transferrin-Binding Proteins