title: Characterization of the interaction of galactose-exposing particles with rat Kupffer cells.
abstract: The characteristics of the recognition system involved in the binding of galactose-exposing particles to freshly isolated rat Kupffer cells were determined. For this purpose we used iodinated lactosylated low-density lipoprotein (125I-Lac-LDL) as a ligand for the galactose receptor on Kupffer cells. The affinity of the binding of 125I-Lac-LDL to Kupffer cells was saturable (23,500 galactose-specific binding sites per cell) and of high affinity (2.4 +/- 0.3 nM). The order of potency of various carbohydrates in inhibiting the association of 125I-Lac-LDL with Kupffer cells was as follows: N-acetylgalactosamine > L-fucose >> N-acetylglucosamine/mannan. Association of 125I-Lac-LDL with Kupffer cells in the absence of Ca2+ was at the same level as in the presence of 50 mM N-acetylgalactosamine. A polyclonal antibody raised against the rat asialoglycoprotein receptor inhibited the binding of 125I-Lac-LDL to Kupffer cells and reacted in a Western blot with two proteins (molecular mass 88 and 77 kDa), which correspond to the molecular mass of the fucose receptor [Lehrman, Haltiwanger and Hill (1986) J. Biol. Chem. 261, 7426-7432]. Furthermore, the ability of fucosylated neoglycoproteins to displace 125I-Lac-LDL from Kupffer cells was equally dependent on the extent of fucosylation as previously reported for the fucose receptor. We conclude that the fucose receptor and not the C-reactive protein, as recently proposed [Kempka, Roos and Kolb-Bachofen (1990) J. Immunol. 144, 1004-1009], functions as the galactose-particle receptor on the Kupffer cell. The binding of galactose-exposing particles to the fucose receptor is a previously unknown property of this receptor.
authors.affiliation: Division of Biopharmaceutics, Leiden Amsterdam Center for Drug Research, Sylvius Laboratory, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Kuiper
authors.forename: H F
authors.initials: HF
authors.lastname: Bakkeren
authors.forename: E A
authors.initials: EA
authors.lastname: Biessen
authors.forename: T J
authors.initials: TJ
authors.lastname: Van Berkel
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism; D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002097:C-Reactive Protein / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D006801:Humans; D007728:Kupffer Cells / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008351:Mannans / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
chemical_list: D008077:Lipoproteins, LDL; D008351:Mannans; D005643:Fucose; D002097:C-Reactive Protein; D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D005690:Galactose