Two immunodominant regions revealed by monoclonal antibodies on the main structural protein p24 of bovine leukemia virus.
Eleven different monoclonal antibodies (Mabs) directed against the main structural protein p24 of bovine leukemia virus (BLV) were prepared. All Mabs reacted with p24 in Western blot and in radioimmunoprecipitation. Competition antibody binding assays with the prepared Mabs distinguished three independent groups of Mabs. Two immunodominant regions (IDRs) of p24 BLV were defined by these Mabs. The Mabs were induced preferentially against two immunodominant regions on the native form of p24 BLV (BLVp24 IDR-1 and BLVp24 IDR-2). Mab of the third group was directed against a different immunogenic epitope of p24 BLV. A model of the IDRs based on the differences in the fine epitope specificity of Mabs defining these immunodominant regions is proposed.
Orlik
O
O
Department of Molecular Virology, Slovak Academy of Sciences, Bratislava.
Ban
J
J
Gieciova
E
E
Altanerova
V
V
Altaner
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D016056:Immunodominant Epitopes / Q000276:immunology*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D014758:Viral Core Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D016056:Immunodominant Epitopes; D014758:Viral Core Proteins