An analog of MIF, alaptide: effect on serum prolactin, dopamine receptors and growth of rat adenohypophysis.
Alaptide is a drug chemically derived from prolyl-leucyl-glycin amide (PLG) which is effective after oral administration. We studied the effect of long term treatment with alaptide and estradiol-benzoate on serum prolactin, growth reactivity and dopamine DA-2 receptors in the anterior pituitary of male rats. Alaptide reduced adenohypophyseal weight when given alone, but only nonsignificantly reduced growth reactivity of the anterior pituitary (AP) raised by estradiol-benzoate (EB). Alaptide significantly decreased the serum level of prolactin but, on the other hand, significantly increased the binding of 3H-spiperone to dopamine DA-2 receptors in AP membrane preparations, without affecting affinity. The administration of alaptide plus EB together resulted in an additional increase of dopamine DA-2 binding sites. We assume that alaptide has a weak dopaminergic activity on the AP of male rats.
Nedvídková
J
J
Institute of Endocrinology, Prague, Czech Republic.
Kasafírek
E
E
Nedvídek
J
J
Pacák
K
K
Schreiber
V
V
D000818:Animals; D004357:Drug Synergism; D004958:Estradiol / Q000494:pharmacology; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011388:Prolactin / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000378:metabolism*
D009479:Neuropeptides; D010456:Peptides, Cyclic; D017448:Receptors, Dopamine D2; D004958:Estradiol; D011388:Prolactin; C049178:cyclo(alanine-(1-amino-1-cyclopentane)carbonyl)