Alternative RNA splicing of calcitonin/calcitonin gene-related peptide minigene transcripts in a thyroid C-cell line.
RNA transcripts derived from the calcitonin (CT)/calcitonin gene-related peptide (CGRP) gene are differentially processed in a tissue-specific fashion to produce two unique mRNAs. This RNA processing decision is deregulated in malignant thyroid C-cells. To examine this mechanism of RNA processing, CT/CGRP minigene constructs were transfected into the human medullary thyroid carcinoma TT cell line. RNA derived from the normal CT/CGRP construct paralleled the endogenous pathway to produce both CT and CGRP mRNAs. Mutation analysis and RNA/protein crosslinking were performed in order to clarify trans-acting factor interactions. The data suggest that CGRP production in TT cells results from the coexpression of facilitative and inhibitory factors.
Cote
G J
GJ
Department of Medical Specialties, University of Texas M.D. Anderson Cancer Center, Houston 77030.
D017398:Alternative Splicing / Q000235:genetics; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000378:metabolism
D017931:DNA Primers; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide