title: Myeloblastin/proteinase 3 belongs to the set of negatively regulated primary response genes expressed during in vitro myeloid differentiation.
abstract: Regulation of myeloblastin expression was studied during myeloid differentiation of HL60 cells and compared with that of myeloperoxidase. Induction towards the monocytic pathway of differentiation caused a rapid, marked and parallel down-regulation of both transcripts which were insensitive to cycloheximide. Thus, myeloblastin is directly regulated and, similarly to myeloperoxidase, belongs to the set of primary response genes. Nuclear run-on experiments and RNA half-life determinations revealed that myeloblastin regulation was at both the transcriptional and posttranscriptional levels.
authors.affiliation: Laboratory for Clinical and Experimental Research, University of Berne, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Meier
authors.forename: A
authors.initials: A
authors.lastname: Ziemiecki
authors.forename: M F
authors.initials: MF
authors.lastname: Fey
authors.forename: A
authors.initials: A
authors.lastname: Tobler
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005819:Genetic Markers; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D006410:Hematopoiesis / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D053146:Myeloblastin; D009195:Peroxidase / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers; D012333:RNA, Messenger; D009195:Peroxidase; D012697:Serine Endopeptidases; D053146:Myeloblastin; D013755:Tetradecanoylphorbol Acetate