title: Demonstration of epidermal growth factor receptor in colorectal adenocarcinoma by enzyme immunoassay.
abstract: The epidermal growth factor receptor (EGFR) was investigated by an enzyme immunoassay in neoplastic tissue and surrounding mucosa from 43 patients with colorectal cancer. This study found a positivity of 86% in normal samples and a positivity of 93% in neoplastic ones. In normal tissue samples, EGFR levels were 162 +/- 129 ng Ab B/mg protein, while in colorectal neoplastic samples EGFR levels were 228 +/- 135 ng Ab B/mg protein. This difference was statistically significant (p < 0.01). There was a significant correlation between the EGFR levels in neoplastic tissue and those in surrounding mucosa (r = 0.75). In 5 of 43 patients, the EGFR was evaluated also by Western blotting with three different anti-EGFR antibodies, of which one was biotin-conjugated. The presence of a 170-kDa band was detected. Moreover, by using the anti-EGFR mAb biotin conjugate a 90-kDa band was also detected.
authors.affiliation: Laboratory of Biochemistry, I.R.C.C.S. S. de Bellis, Castellana G. (BA), Italy.
authors.forename: C
authors.initials: C
authors.lastname: Messa
authors.forename: F
authors.initials: F
authors.lastname: Russo
authors.forename: M
authors.initials: M
authors.lastname: Notarnicola
authors.forename: A
authors.initials: A
authors.lastname: Di Leo
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D066246:ErbB Receptors / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight
chemical_list: D000911:Antibodies, Monoclonal; D066246:ErbB Receptors