title: Isolation and characterization of Bordetella parapertussis-like bacteria from ovine lungs.
abstract: Bacteria resembling two Bordetella species were isolated from both normal and pneumonic ovine lungs using a selective charcoal agar. Twenty-eight of the 33 isolates showed similarities to stock NCTC B. parapertussis strains in their SDS-PAGE gel protein profiles, in their biochemical reactions and in causing browning on tyrosine agar. Five isolates behaved similarly to stock B. bronchiseptica strains, in being actively motile, in giving identical positive reactions in three out of four biochemical tests and in causing no colour change in tyrosine agar. Multilocus enzyme electrophoresis separated the isolates into two electrophoretic types distinguishable from those of stock B. parapertussis and stock B. bronchiseptica strains.
authors.affiliation: Moredun Research Institute, Edinburgh, UK.
authors.forename: J F
authors.initials: JF
authors.lastname: Porter
authors.forename: K
authors.initials: K
authors.lastname: Connor
authors.forename: W
authors.initials: W
authors.lastname: Donachie
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001884:Bordetella / Q000145:classification / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000382:microbiology / Q000662:veterinary*; D016950:Bordetella bronchiseptica / Q000145:classification; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000032:analysis; D008168:Lung / Q000382:microbiology*; D011014:Pneumonia / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep / Q000382:microbiology*; D012757:Sheep Diseases / Q000382:microbiology*; D015047:Zoonoses
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes