Human embryonic behavior in a sequential human oviduct-endometrial coculture system.
Embryonic behavior to blastocyst, hatching, and hatched stages were evaluated in 77, four-cell human embryos that were first grown in oviductal cell coculture and then equally allotted at the eight-cell stage to two coculture systems in a serum-free medium (34 continued on oviductal monolayers, 32 on endometrium monolayers). Sixty-three percent and 40% of embryos expanded and hatched in the sequential oviductal-endometrial coculture system when compared with 41% and 9% in the oviductal system alone, respectively. The sequential coculture system appears to be an improved system over the single human oviductal coculture system.
Bongso
A
A
Department of Obstetrics and Gynaecology, National University Hospital, Kent Ridge, Singapore.
Fong
C Y
CY
Ng
S C
SC
Ratnam
S
S
D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000502:physiology*; D004717:Endometrium / Q000502:physiology*; D005187:Fallopian Tubes / Q000502:physiology*; D005260:Female; D006801:Humans