Potential glucose sensor for perioperative blood glucose control in diabetes mellitus.
A glucose sensor intended for future use in diabetic patients undergoing major surgery is described. It involves glucose oxidase immobilised at a platinised activated carbon electrode (PACE). The sensor gave a nonoxygen dependent amperometric response in whole undiluted blood and did not require the use of electron mediators. In vitro studies in protein containing buffer using a flow cell indicated current densities of approximately 160 nA mm-2 mM-1 and a linear response over the range 0-20 mM. The operational stability of the sensor was at least 49 h in continuous use. In addition the sensor had a 90-99% response time of 1 min when used at a flow rate of 3 ml min-1 and showed a temperature dependence of 2.4% C-1. The results reported suggest significant advantages of this approach, for future use as a perioperative intra-vascular sensor for diabetic subjects, over previously reported glucose sensors.
Greenough
K R
KR
Department of Clinical Biochemistry, Medical School, University of Newcastle upon Tyne, UK.
Skillen
A W
AW
McNeil
C J
CJ
D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D003920:Diabetes Mellitus / Q000097:blood*; D004795:Enzyme Stability; D005949:Glucose Oxidase; D006801:Humans; D016014:Linear Models; D016343:Monitoring, Intraoperative / Q000379:methods*; D015203:Reproducibility of Results
D001786:Blood Glucose; D005949:Glucose Oxidase