Cytokine-mediated induction of HLA antigen expression on human glomerular mesangial cells.
Well-characterized human glomerular mesangial cells were stimulated in vitro with interferon-gamma (IFN-gamma), interleukin-1, and tumor necrosis factor alpha (TNF-alpha) to determine the influence of these cytokines on the expression of class I and II HLA antigens. IFN-gamma induced a dose-dependent increase in expression of both class I and class II HLA antigens. TNF-alpha induced only class I antigens when used alone, but acted synergistically with IFN-gamma in the expression of class I and II antigens. Interleukin-1 had little effect on HLA antigen expression. These results raise the possibility that stimulated mesangial cells may act as antigen-presenting cells and may mediate local immune reactions in the glomerulus.
Hawkins
N J
NJ
School of Pathology, University of New South Wales, Kensington, Australia.
Ward
R L
RL
Wakefield
D
D
D000938:Antigen-Presenting Cells / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D005434:Flow Cytometry; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D006680:HLA Antigens / Q000096:biosynthesis*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016207:Cytokines; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma