Muscarinic receptor-mediated increase in zeta-PKC expression in SK-N-SH human neuroblastoma cells.
Anti-peptide antibodies specific for each protein kinase C (PKC) isozyme were used to screen SK-N-SH human neuroblastoma cells. These cells were found to express only alpha- and zeta-PKC. Stimulation of these cells with phorbol esters caused alpha- but not zeta-PKC to translocate from cytosolic to membrane fractions. Stimulation of these cells with carbachol, which releases inositol trisphosphate and diacylglycerol, caused a transient translocation of alpha-PKC but not of zeta-PKC. Carbachol did, however, cause a gradual increase in immunoreactive zeta-PKC which reached maximal values 10-20 min after stimulation. These results implicate zeta-PKC in a receptor-mediated signalling event.
Baumgold
J
J
Department of Radiology, George Washington University Medical Center, Washington, D.C. 20037.
Dyer
K D
KD
D015153:Blotting, Western; D002217:Carbachol / Q000494:pharmacology; D004789:Enzyme Activation; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D009447:Neuroblastoma / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000032:analysis*; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D010703:Phorbol Esters; D011976:Receptors, Muscarinic; D002217:Carbachol; D011493:Protein Kinase C