[Evaluation of a new rapid ELISA technique for detecting human cytomegalovirus antibodies (CMV). Applications in emergency blood transfusion and in organ transplantation].
Immunocubes can detect very low HCMV IgG levels with at least the same sensitivity as the reference technique: ELISA. Samples containing bacteria showed no particular interference on the test, and results for non-infected material were in agreement with those of ELISA. The rapid test did not give any false positive reactions even with samples from patients with very high antiherpes immunoglobulins levels. Samples from transplant patients at the beginning of seroconversion or diluted to IgG titers corresponding to ELISA cut-off values were always detected as positive by immunocubes. In view of the rapidity of the test, its high specificity, its ability to detect levels corresponding to extremely low IgG signals in ELISA, and the possibility of conserving objective proof of the test, we conclude that this rapid immunocube technique is of great interest for current serologic screening in an emergency context, especially for transfusion and organ transplantation.
Goldschmidt
P
P
Service de virologie, hôpital Broussais, Paris, France.
Matta
M
M
Cotigny
S
S
Longuet
M F
MF
Aimé
C
C
Biglione
M
M
Guillemain
R
R
Amrein
C
C
Farge
D
D
D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D001803:Blood Transfusion; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016027:Heart Transplantation; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Evaluation d'une nouvelle technique rapide par Elisa de dépistage des anticorps anticytomégalovirus humain (CMV). Applications pour les transfusions sanguines en urgence et dans un contexte de transplantation d'organes.