title: [A new method of immobilizing proteolytic enzymes in polymeric hydrogels].
abstract: A novel one-step method for enzyme immobilization in composite gels based on the thermally reversible polymer poly (N-vinyl-caprolactam) was developed. Conditions for entrapment of trypsin and carboxypeptidase B in the gels were optimized. After immobilization, 80 and 90% of the original carboxypeptidase B and trypsin activities, respectively, were found to retain. Both immobilizes enzymes were active in a wider pH and temperature range. The granules with entrapped enzymes were successfully applied to obtain human insulin from recombinant proinsulin.
authors.forename: E A
authors.initials: EA
authors.lastname: Markvicheva
authors.forename: A S
authors.initials: AS
authors.lastname: Bronin
authors.forename: N E
authors.initials: NE
authors.lastname: Kudriavtseva
authors.forename: I F
authors.initials: IF
authors.lastname: Kuz'kina
authors.forename: I I
authors.initials: II
authors.lastname: Pashkin
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Kirsh
authors.forename: L D
authors.initials: LD
authors.lastname: Rumsh
authors.forename: V P
authors.initials: VP
authors.lastname: Zubov
mesh_terms: D002209:Caprolactam / Q000031:analogs & derivatives*; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D004800:Enzymes, Immobilized; D006801:Humans; D006868:Hydrolysis; D011108:Polymers; D011384:Proinsulin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D011994:Recombinant Proteins; C086861:poly-N-vinylcaprolactam; D002209:Caprolactam; D011384:Proinsulin; D002268:Carboxypeptidases; D010450:Endopeptidases; D043424:Carboxypeptidase B; D014357:Trypsin
vernacular_title: Novyĭ metod immobilizatsii proteoliticheskikh fermentov v polimernykh gidrogeliakh.