[Enzyme immunoassay of potassium oxonate using specific antibody isolated by immunosorbent gel].
An enzyme immunoassay for the determination of potassium oxonate in the plasma has been developed. The procedure is based on a competitive enzyme-linked immunosorbent assay using the second antibody solid phase method. The antiserum for potassium oxonate was prepared using oxonic acid 6-carboxypentylamide-BSA conjugate as immunogen. The specific antibody for oxonic acid was isolated from the antiserum using oxonic acid 6-carboxypentylamide immobilized immunosorbent gel. The purified antibody resulted in high sensitivity and low cross-reactivity as compared with the unpurified antiserum. Potassium oxonate in the plasma could be assayed in the range from 20 to 1000 ng/ml by the proposed EIA. The recovery was ranged from 82 to 117% and the coefficient of variation was from 6.6 to 14.7% (n = 6).
Kitamura
R
R
Pharmacokinetics Research Laboratory, Taiho Pharmaceutical Co., Ltd., Tokushima, Japan.
Satoh
T
T
Maeda
M
M
Tsuji
A
A
D000918:Antibody Specificity; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005782:Gels; D006801:Humans; D007106:Immune Sera; D007164:Immunosorbents; D010094:Oxonic Acid / Q000097:blood*
D005782:Gels; D007106:Immune Sera; D007164:Immunosorbents; D010094:Oxonic Acid