[Oligonucleotide directed in vitro mutagenesis].
In this study, a method for oligonucleotide directed in vitro mutagenesis was described. It includes the following steps: clone of the gene to be mutated into the phagemid pGCI; preparation of single strand template; design and synthesis of mutated oligonucleotides; synthesis of double strand DNA. Using this method, a new BamHI site was originated at the N terminal of Stx-B and BglII site at the C terminal of Stx-B. It is ready to fuse the Stx-B gene to LamB. This method is a very useful tool for genetic engineering.
Su
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Institute of Biotechnology, Academy of Military Medical Sciences, Beijing.
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides; D010957:Plasmids
D009841:Oligonucleotides