Activation of eukaryotic transcriptional promoters by the bovine papillomavirus E1-replication factor.
It has been suggested that the bovine papillomavirus type 1 E1 replication factor may inhibit E2-conditional gene expression from viral promoter P89. To study the possible role of the E1 protein in gene expression, HeLa cells were transfected with E2-conditional or E2-independent reporter plasmids and with vectors expressing the E1 and E2 open reading frames. The data show that: (i) replication factor E1 stimulates gene expression from a variety of eucaryotic transcriptional promoters; (ii) activation of gene expression by E1 also occurs in the absence of viral activator E2-TA; (iii) expression of a recombinant plasmid containing the human papillomavirus type 18 origin of DNA replication is stimulated, but not repressed, by E1, and (iv) E1-dependent activation of gene expression does not reflect the amplification of transfected plasmids.
Monini
P
P
Institute of Microbiology, University of Ferrara, Italy.
de Lellis
L
L
Borgatti
P
P
Hassan-Omran
M
M
Cassai
E
E
D052688:Bovine papillomavirus 1; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000494:pharmacology*
D004268:DNA-Binding Proteins; C069814:E1 protein, Bovine papillomavirus; C051550:E2 protein, Bovine papillomavirus; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D015500:Chloramphenicol O-Acetyltransferase