title: The value of routine screening of female serum for antisperm antibodies in assisted reproductive technology cycles.
abstract: OBJECTIVE
  To determine the effect of antisperm antibodies in the female serum on fertilization and pregnancy rates (PRs) in assisted reproductive technology (ART) cycles.
  
  
  METHODS
  Retrospective case control study.
  
  
  METHODS
  Assisted reproductive technology program at North Shore University Hospital.
  
  
  METHODS
  All patients undergoing an ART cycle in 1990 whose husbands did not have significant antisperm antibodies in the semen. Thirty-four female patients had significant antisperm antibodies level in the serum (antisperm antibodies-significant); 62 patients with no antibodies or nonsignificant levels constituted the control group (antisperm antibodies-negative). Incubation media were prepared with maternal serum in 37 of the antisperm antibodies-negative and in 10 of the antisperm antibodies-positive patients, whereas donor's serum (DS) was used for 25 and 24 patients, respectively.
  
  
  METHODS
  Fertilization rate and clinical PR, defined as an intrauterine sac by transvaginal ultrasonography, were recorded for each group.
  
  
  RESULTS
  Analysis of variance showed a significant interaction between antisperm antibodies grouping and the type of media used, with fertilization rate in antisperm antibodies-significant patients significantly higher with maternal serum than with DS, whereas fertilization rate in antisperm antibodies-negative was quantitatively, though not statistically lower with maternal serum than with DS. When maternal serum was used, fertilization was higher in antisperm antibodies-significant than antisperm antibodies-negative. Of 29 clinical pregnancies, 11 were in antisperm antibodies-significant and 18 in antisperm antibodies-negative. In 10 of the pregnancies, maternal serum was used, whereas in 19 pregnancies DS was used. No variable was significantly predictive of pregnancy in the logistic regression analysis.
  
  
  CONCLUSIONS
  [1] Female patients with significant levels of antisperm antibodies in the serum had similar fertilization rates as patients with nonsignificant levels if DS was used. [2] The use of maternal serum in antisperm antibodies-significant patients did not result in inferior fertilization rates or the occurrence of pregnancy. [3] These findings suggest that a female antisperm antibodies may not hinder fertilization in vitro. [4] This study questions the value of routine screening of female serum for antisperm antibodies in ART.
authors.affiliation: Department of Obstetrics and Gynecology, North Shore University Hospital, Manhasset, New York 11030.
authors.forename: A
authors.initials: A
authors.lastname: Hershlag
authors.forename: B
authors.initials: B
authors.lastname: Napolitano
authors.forename: C
authors.initials: C
authors.lastname: Cangemi
authors.forename: G
authors.initials: G
authors.lastname: Scholl
authors.forename: D
authors.initials: D
authors.lastname: Rosenfeld
mesh_terms: D000328:Adult; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D003955:Diagnostic Tests, Routine; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D015994:Incidence; D008297:Male; D011247:Pregnancy / Q000502:physiology; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D000906:Antibodies