[Molecular study of the mechanism of chromosomal translocation (15;17) in acute promyelocytic leukemia (APL)].
Molecular studies of chromosomal translocation (15;17) in acute promyelocytic leukemia (APL) have shown that retinoic acid receptor A (RARA) gene on chromosome 17 is juxtaposed to the PML gene on chromosome 15. This results in a PML-RARA chimeric gene. Our work has demonstrated that the PML breakpoints in APL patients are clustered in two limited regions, PML-bcr1 and PML-bcr2, separated from each other by about 10 kb. DNA sequence of PML-bcr1 and primary structure of the junctional region of reciprocal chromosomal translocation in a patient have been determined in this paper. Compared to those of two previously reported cases abroad, we found that the breakpoint may be situated in the topoisomerase II cleavage site. A working model has been proposed for the mechanism of DNA illegitimate recombination in t (15;17).
Dong
S
S
Laboratory of Molecular Biology, Shanghai Rui-Jin Hospital, Shanghai Second Medical University.
Tong
J
J
Wu
Y
Y
Cai
J
J
Sun
G
G
Chen
S
S
Wang
Z
Z
Chen
S
S
Chen
Z
Z
Geng
J
J
D001483:Base Sequence; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008969:Molecular Sequence Data; D014178:Translocation, Genetic