Application of polymorphic DNA sequences to differentiate the origin of decomposed bovine meat.
Two bovine meat samples were analysed although the samples were already in a state of advanced decomposition. The isolated DNA was extremely degraded and not suitable for conventional DNA fingerprinting (profiling), thus the polymerase chain reaction (PCR) was applied to screen for a Y-chromosome specific fragment and the loci of the two milk proteins kappa-casein and beta-lactoglobulin. Both samples contained Y-chromosome specific DNA and the kappa-casein genotype AA but they differed at the beta-lactoglobulin locus (BB versus AB). Thus a different origin (carcass) could be verified for the two samples and a subject was exonerated from a case of meat larceny.
Wagner
V
V
Department of Animal Breeding, Hohenheim University, Stuttgart, Germany.
Schild
T A
TA
Geldermann
H
H
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005784:Gene Amplification; D005838:Genotype; D008894:Milk Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011180:Postmortem Changes; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014998:Y Chromosome / Q000737:chemistry
D008894:Milk Proteins