[Cleavage of genomic DNA along the boundaries of topologic domains by nuclear matrix topoisomerase II].
A new method for mapping of eukaryotic genome domain organization was developed. At the main step of the method, insoluble form of topoisomerase II from nuclear matrix specifically digested genome DNA at junctions of topological domains. Domain organization of cluster of mammalian ribosomal genes was characterized using this method. The genes were shown to form loops, equal to individual repeat in length. The loops alternated nuclear matrix attached DNA fragments, located in non-transcribed spacer upstream 18S RNA transcription initiation site. Hybridization of specific probes with electrophoretically fractionated DNA fragments, resulted from topoisomerase II (insoluble form) activity, enabled to determine the size of loops, containing a number of unique genes.
Iarovaia
O V
OV
Razin
C V
CV
Khénkok
R
R
D000818:Animals; D016466:CHO Cells; D002463:Cell Membrane Permeability; D006224:Cricetinae; D004275:DNA, Ribosomal / Q000235:genetics; D016678:Genome; D008970:Molecular Weight; D005810:Multigene Family; D015530:Nuclear Matrix / Q000201:enzymology; D009690:Nucleic Acid Conformation; D009710:Nucleotide Mapping; D011034:Podophyllotoxin; D059005:Topoisomerase II Inhibitors
D004275:DNA, Ribosomal; D059005:Topoisomerase II Inhibitors; D011034:Podophyllotoxin
Rasshcheplenie genomnoĭ DNK po granitsam topologicheskikh domenov topoizomerazoĭ II iadernogo matriksa.