Carpal and tarsal bone anomalies in mice induced by maternal treatment of Ara-C.
Pregnant mice of the Jcl:ICR strain were injected intraperitoneally with a single dose of 5 mg/kg of cytosine arabinoside (Ara-C) on dg 10.5. Skeletal changes of the forepaw and hindpaw were evaluated in the offspring on the 15th or 24th postnatal day. Various anomalies of carpal and tarsal bones such as fusion, absence, and deformation occurred at high incidence at a dose that produced digital anomalies. Almost all newborns with Ara-C-induced digital anomalies (oligodactyly or polydactyly) had anomalies of carpal or tarsal bones in the particular paw, while anomalies of carpal or tarsal bones could occur in the presence or absence of digital anomalies. Carpal and tarsal bone anomalies may become a sensitive and novel parameter of limb development in the postnatal teratogen testing system.
Rahman
M E
ME
Department of Hygiene and Preventive Medicine, Yamagata University School of Medicine, Japan.
Ishikawa
H
H
Watanabe
Y
Y
Endo
A
A
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D002348:Carpal Bones / Q000002:abnormalities* / Q000187:drug effects; D003561:Cytarabine / Q000633:toxicity*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D013639:Tarsal Bones / Q000002:abnormalities* / Q000187:drug effects; D013723:Teratogens / Q000633:toxicity*
D013723:Teratogens; D003561:Cytarabine