Expression of pS2 protein in breast cancer.
The pS2 protein was first detected in the oestrogen-dependent breast cancer cell line MCF-7. It may have prognostic value for primary breast cancer, and could be used to predict clinical responsiveness to endocrine therapy. In a retrospective study, the concentrations of pS2 protein were determined in 434 cytosol specimens using an immunoradiometric assay. The median values (4, 6 and 3 ng/mg protein) and the third quartiles (27, 26 and 29 ng/mg) in benign breast tumours (n = 17), and in primary (n = 325) and recurrent (n = 37) breast carcinomas were of the same order of magnitude. In primary breast cancer, high pS2 values (> 26 ng/mg) correlated significantly with a positive oestrogen receptor (ER) status and a high grade of tumour differentiation (P = 0.01). As many as 85% of the pS2 positive tumours were ER positive. A marginally significant correlation (P = 0.06) was also found between pS2 status and the quantitative expression of the ER. However, the pS2 values in ER positive endometrial carcinomas (n = 12) as well as in other benign and malignant genital tumours (n = 43) were more than 30 times lower than those measured in breast tumours. The results reveal a close association between pS2 protein and ER status which appears to be limited to breast cancer.
Crombach
G
G
Department of Obstetrics and Gynecology, University of Köln, Germany.
Ingenhorst
A
A
Göhring
U J
UJ
Scharl
A
A
Neuhaus
W
W
Möbus
V
V
Schaeffer
H J
HJ
D001706:Biopsy; D001943:Breast Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D004967:Estrogens; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005833:Genital Neoplasms, Female / Q000737:chemistry / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis*; D009364:Neoplasm Recurrence, Local / Q000737:chemistry* / Q000401:mortality / Q000473:pathology*; D009367:Neoplasm Staging; D011379:Prognosis; D011506:Proteins; D011960:Receptors, Estrogen / Q000032:analysis; D012189:Retrospective Studies; D013048:Specimen Handling; D015996:Survival Rate; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins
D004967:Estrogens; D009363:Neoplasm Proteins; D011506:Proteins; D011960:Receptors, Estrogen; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins