Detection of influenza hemagglutinin interaction with biological membranes by photosensitized activation of [125I]iodonaphthylazide.
Fusion of influenza virus with cells is triggered by a pH-dependent conformational change in the viral envelope protein, hemagglutinin, which results in exposure of the fusion peptide and its insertion into the target membrane. We have investigated the association of hemagglutinin with erythrocyte membranes by photosensitized labeling with [125I]iodonaphthylazide. This technique relies on the collisional energy transfer from a photosensitizing chromophore to [125I]iodonaphthylazide, which selectively labels proteins in the vicinity of the chromophore. Incubation of influenza virus with erythrocyte membranes containing chromophore and [125I]iodonaphthylazide results in labeling of hemagglutinin under fusogenic conditions (pH 5 and 37 degrees C). We also examined photosensitized labeling of hemagglutinin upon incubation of the X31 strain of influenza virus with labeled erythrocyte membranes in a pre-fusion state (pH 5 and 4 degrees C). There was little hemagglutinin labeling under these conditions, although incubation of bromelain-cleaved hemagglutinin, which lacks the transmembrane region, resulted in rapid labeling. Hemagglutinin was also labeled by [125I]iodonaphthylazide photosensitized by a fluorescent substrate transported through the erythrocyte band 3 sialoglycoprotein. Hemagglutinin labeling decreased after an initial rapid rise, suggesting that the fusion site is close to the sialoglycoprotein and that [125I]iodonaphthylazide photosensitized labeling may be used to assay protein movement during fusion.
Pak
C C
CC
Section of Membrane Structure and Function, NCI, National Institutes of Health, Bethesda, Maryland 20892.
Krumbiegel
M
M
Blumenthal
R
R
Raviv
Y
Y
D001386:Azides / Q000378:metabolism*; D002458:Cell Fractionation; D004910:Erythrocyte Membrane / Q000502:physiology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D007457:Iodine Radioisotopes; D007700:Kinetics; D008561:Membrane Fusion; D010069:Oxadiazoles; D010777:Photochemistry; D011485:Protein Binding; D013654:Taurine / Q000031:analogs & derivatives; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000378:metabolism*
D001386:Azides; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D007457:Iodine Radioisotopes; C027027:NBD-taurine; D010069:Oxadiazoles; D014759:Viral Envelope Proteins; D013654:Taurine; C015298:iodonaphthylazide