Capillary zone electrophoresis separation of kinins using a novel laser fluorescence detector.
1. Bradykinin, Lys-bradykinin, Met-Lys-bradykinin, des-Arg9-bradykinin and des-Arg1-bradykinin were separated by capillary zone electrophoresis in an apparatus constructed in our laboratory which utilizes a novel N2 pulsed laser-induced fluorescence detector. 2. Detection limits of 1.2 fmol for fluorescamine-derivatized bradykinin and 90 attomol for O-phthaldialdehyde-derivatized bradykinin were achieved. 3. This powerful analytical tool is described and its successful application to the measurements of bradykinin after enzymatic release from blood is documented.
Kist
T B
TB
Instituto de Física, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Termignoni
C
C
Grieneisen
H P
HP
D000818:Animals; D002417:Cattle; D004586:Electrophoresis / Q000379:methods*; D005453:Fluorescence; D007705:Kinins / Q000302:isolation & purification*; D007834:Lasers; D013997:Time Factors
D007705:Kinins