title: Presequence-mediated intermembrane contact formation and lipid flow. A model membrane study.
abstract: The ability of a synthetic peptide corresponding to the presequence of cytochrome c oxidase subunit IV from yeast to cause intermembrane contacts was investigated using monolayer techniques. The presequence inserted efficiently into the monolayer with a specificity for the mitochondrial cardiolipin. In the inserted form, the peptide strongly promoted the formation of close contacts with large unilamellar lipid vesicles present in the subphase, a property which was also specific for cardiolipin. The contacts formed were stable and tight and resulted in the flow of lipids from the vesicles to the monolayer. These results led to new suggestions on the involvement of intermembrane contact formation in mitochondrial protein import and membrane biogenesis.
authors.affiliation: Department of Biochemistry of Membranes, Utrecht University, The Netherlands.
authors.forename: Z
authors.initials: Z
authors.lastname: Török
authors.forename: R A
authors.initials: RA
authors.lastname: Demel
authors.forename: J M
authors.initials: JM
authors.lastname: Leenhouts
authors.forename: B
authors.initials: B
authors.lastname: de Kruijff
mesh_terms: D000595:Amino Acid Sequence; D003576:Electron Transport Complex IV / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008567:Membranes, Artificial; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000378:metabolism*
chemical_list: D008563:Membrane Lipids; D008567:Membranes, Artificial; D021382:Protein Sorting Signals; D003576:Electron Transport Complex IV