Variation of transferrin mRNA concentration in the rabbit mammary gland during the pregnancy-lactation-weaning cycle and in cultured mammary cells. A comparison with the other major milk protein mRNAs.
The concentration of transferrin mRNA was evaluated during pregnancy and lactation in rabbit mammary gland and liver using northern blot and dot blot assays. Transferrin mRNA was present in the virgin rabbit mammary gland and its concentration increased as pregnancy proceeded, with a major enhancement after day 15. A high concentration was reached 3 days after parturition, with no additional increase during lactation and with a marked decline after weaning. During the same period, the concentration of transferrin mRNA showed only a very weak variation in liver. This mRNA was six times more abundant in mammary gland than in liver of lactating rabbit. The accumulation of transferrin mRNA in the mammary gland was concomitant with the accumulation of alpha s1-, beta-, kappa-casein and WAP (whey acidic protein) mRNAs. The concentration of glyceraldehyde 3-phosphate dehydrogenase mRNA, taken as a non-inducible control mRNA, declined progressively during pregnancy to reach its lower level in lactation. These observations suggest that casein, WAP and transferrin mRNAs are subjected to a similar control mechanism in vivo, at least in the second half of pregnancy and during lactation. Experiments carried out in vitro using isolated rabbit epithelial mammary cells cultured on collagen I gel indicated that transferrin mRNA was abundant and only weakly inducible by the lactogenic hormones insulin, cortisol and prolactin, as opposed to caseins and WAP mRNAs. R5020, an analogue of progesterone, inhibited at most very slightly the accumulation of alpha s1-casein mRNA in the presence of prolactin and it did not reduce the expression of transferrin gene.(ABSTRACT TRUNCATED AT 250 WORDS)
Puissant
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C
Unité de Differenciation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Bayat-Sarmadi
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M
Devinoy
E
E
Houdebine
L M
LM
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002364:Caseins / Q000096:biosynthesis / Q000235:genetics; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D005109:Extracellular Matrix / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D007774:Lactation / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D008894:Milk Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits / Q000378:metabolism*; D014168:Transferrin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014886:Weaning
D002364:Caseins; D017931:DNA Primers; D008894:Milk Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D014168:Transferrin; C024471:whey acidic proteins; C068142:glyceraldehyde 3-phosphate dehydrogenase (304-313); D005987:Glyceraldehyde-3-Phosphate Dehydrogenases