title: Differences in outward currents between neonatal and adult rabbit ventricular cells.
abstract: In adult rabbit ventricular preparations, action potential duration is significantly increased when stimulation frequency is increased from 0.1 to 1.0 Hz. In neonatal preparations, a similar change in stimulation frequency produced no significant increase in action potential duration. To identify the ionic basis for this difference, we studied different outward currents in single myocytes from papillary muscle and from epicardial tissue of adult and neonatal rabbits. The densities of the outward currents in neonatal cells were about one-half of the current density in adult cells. The density of the voltage-activated transient outward current (I(to1)) was smaller in cells from papillary muscle than in cells from epicardium in adult and newborn rabbits. We found major differences in the kinetic behavior of I(to1) between adult and neonatal cells: 1) the rate of apparent inactivation was faster in neonatal cells, and 2) the recovery from inactivation was significantly faster in neonatal cells, with a time constant of 113 vs. 1,356 ms. We propose that this marked difference in the recovery from inactivation of I(to1) is the basis for the difference in frequency dependence of action potential duration.
authors.affiliation: Centro de Investigaciones Biomédicas, Universidad de Colima, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Sánchez-Chapula
authors.forename: A
authors.initials: A
authors.lastname: Elizalde
authors.forename: R
authors.initials: R
authors.lastname: Navarro-Polanco
authors.forename: H
authors.initials: H
authors.lastname: Barajas
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000200:Action Potentials; D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000502:physiology*; D004594:Electrophysiology; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006352:Heart Ventricles; D010210:Papillary Muscles / Q000166:cytology / Q000502:physiology; D010496:Pericardium / Q000166:cytology / Q000502:physiology; D011817:Rabbits; D011930:Reaction Time
chemical_list: D015761:4-Aminopyridine