title: Investigation of the activation state of the X chromosome in non-Hodgkin's lymphomas.
abstract: Cytogenetic analysis of non-Hodgkin's lymphomas (NHLs) has previously revealed a high incidence of numerical abnormalities involving the X chromosome. We have now used a combination of fluorescence in situ hybridization (FISH) and Southern blot analysis of methylation to examine the activation state of additional X chromosomes in NHL. Although FISH analysis of X chromosome centromeres in interphase nuclei was complicated by a number of factors, such as cell-cycle position, there was evidence that more than one X chromosome was present in the active state in 4/9 NHL. Methylation studies were carried out using the M27 beta probe, which also suggested that more than one activated X chromosome was present in at least 2/7 NHL cases. The two approaches therefore provided evidence that in some cases of NHL, unlike sex-chromosome-syndrome individuals, additional X chromosomes may be present in the active state. These data support the suggestion that NHL-associated oncogenes might be located on the X chromosome.
authors.affiliation: Department of Clinical Oncology, University Medical School, Sheffield, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Grierson
authors.forename: D W
authors.initials: DW
authors.lastname: Hammond
authors.forename: J R
authors.initials: JR
authors.lastname: Goepel
authors.forename: B W
authors.initials: BW
authors.lastname: Hancock
authors.forename: M H
authors.initials: MH
authors.lastname: Goyns
mesh_terms: D015139:Blotting, Southern; D002874:Chromosome Mapping; D015870:Gene Expression; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D008745:Methylation; D012729:Sex Chromosome Aberrations / Q000209:etiology; D014960:X Chromosome