title: Protection of gerbils from amebic liver abscess by immunization with a recombinant protein derived from the 170-kilodalton surface adhesin of Entamoeba histolytica.
abstract: The protozoan parasite Entamoeba histolytica causes extensive morbidity and mortality worldwide through intestinal infection and amebic liver abscess. Here we show that vaccination of gerbils, a standard model for amebic liver abscess, with recombinant proteins derived from the 170-kDa galactose-binding adhesin of E. histolytica and the serine-rich E. histolytica protein or a combination of the two recombinant antigens provides excellent protection against subsequent hepatic challenge with virulent E. histolytica trophozoites.
authors.affiliation: Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: S L
authors.initials: SL
authors.lastname: Stanley
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology*; D005260:Female; D005849:Gerbillinae; D005982:Glutathione Transferase / Q000276:immunology; D008101:Liver Abscess, Amebic / Q000517:prevention & control*; D058619:Maltose-Binding Proteins; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D005982:Glutathione Transferase