[Conservative therapy for retinoblastoma--effect of melphalan on in vitro electroretinogram].
The effect of melphalan (an alkylic anti-cancer drug) on rabbit and human in vitro ERGs was studied to establish the non-toxic concentration of melphalan in chemo-thermotherapy for retinoblastoma. The ERGs were recorded before and 15 min after the perfusate was changed from the control solution to a melphalan-containing solution. Melphalan 10 micrograms/ml and 40 micrograms/ml caused no significant effect on the a-wave, the b-wave, or the oscillatory potential in either the rabbit or the human in vitro ERG. Melphalan 50 micrograms/ml significantly reduced the b-wave, but the change was reversible. Considering the minimum inhibitory concentration of melphalan against retinoblastoma cells on colony assay (4 micrograms/ml), melphalan is one of the most effective drugs for the conservative treatment of retinoblastoma.
Ueda
M
M
Department of Ophthalmology, Kanazawa University School of Medicine, Ishikawa-ken, Japan.
Tanabe
J
J
Suzuki
T
T
Sakai
H
H
Mochizuki
K
K
Kitano
K
K
Inomata
M
M
Kaneko
A
A
D000818:Animals; D003131:Combined Modality Therapy; D004596:Electroretinography / Q000187:drug effects*; D005134:Eye Neoplasms / Q000628:therapy*; D006358:Hot Temperature / Q000627:therapeutic use; D006801:Humans; D066298:In Vitro Techniques; D008558:Melphalan / Q000494:pharmacology* / Q000627:therapeutic use*; D011817:Rabbits; D012175:Retinoblastoma / Q000628:therapy*
D008558:Melphalan