Effect of different preservation solutions on adenine nucleotide content and metabolism in human kidney transplantation.
Differences in purine metabolism produced by three preservation solutions were studied by determining the adenine nucleotide (ATP, ADP, AMP, and IMP) and nucleoside (adenosine, inosine, and hypoxanthine) levels in human kidney cortical biopsies. Forty kidney allografts were studied using University of Wisconsin (UW) solution (n = 20), Euro-Collins (EC) solution (n = 12), and modified EC solution with mannitol (M; n = 8). No significant differences were found between the three solutions studied with regard to ATP, ADP, or AMP changes. The mean ATP level (nmol/mg prot +/- SEM) at the end of preservation in the UW group was 2.7 +/- 0.3 nmol/mg, in the EC group 3.8 +/- 0.7 nmol/mg, and in the M group 2.3 +/- 0.4 nmol/mg. ATP 30 min after reperfusion in the UW, EC, and M groups was 5.7 +/- 0.8 nmol/mg, 6.4 +/- 1.0 nmol/mg, and 4.6 +/- 0.5 nmol/mg, respectively. However, an important difference appeared in the catabolic products determined. Kidneys perfused with UW solution had a significantly higher level of adenosine (2.6 +/- 0.6 nmol/mg), inosine (11.8 +/- 2.2 nmol/mg), and hypoxanthine (18.1 +/- 2.1 nmol/mg) at the end of cold storage than those perfused with EC (0.4 +/- 0.1 nmol/mg, 2.0 +/- 0.8 nmol/mg, and 7.1 +/- 1.4 nmol/mg) and M solutions (0.2 +/- 0.05 nmol/mg, 0.5 +/- 0.1 nmol/mg, and 5.2 +/- 0.6 nmol/mg; P < 0.05). These levels returned to initial values 30 min postreperfusion and there were no differences with the EC or M solution groups at that time.(ABSTRACT TRUNCATED AT 250 WORDS)
Vigués
F
F
Department of Urology, Hospital Prínceps d'Espanya, L'Hospitalet del Llobregat, Barcelona.
Ambrosio
S
S
Bartrons
R
R
Campillo
M
M
Riera
L
L
Lopez
M A
MA
Griño
J M
JM
Serrallach
N
N
Franco
E
E
D000227:Adenine Nucleotides / Q000378:metabolism*; D000241:Adenosine / Q000494:pharmacology; D000328:Adult; D000493:Allopurinol / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004734:Energy Metabolism; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation; D008353:Mannitol / Q000494:pharmacology; D009705:Nucleosides / Q000378:metabolism; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011887:Raffinose / Q000494:pharmacology; D014184:Transplantation, Homologous
D000227:Adenine Nucleotides; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D009705:Nucleosides; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose