title: Depressed neutrophil chemotaxis in children suffering blunt trauma.
abstract: OBJECTIVE
  Impaired neutrophil (PMN) function, due in part to release of immature PMNs into the circulation, contributes to the increased rate of infection observed in adults suffering blunt trauma. The objective of this study was to determine whether similar events occur in children.
  
  
  METHODS
  We assessed PMN chemotaxis and PMN maturation in 25 children (7 young children and 18 adolescents) and 25 adults 1 to 9 days after suffering blunt trauma, and in healthy adult control subjects. PMN chemotaxis was determined using a standard micropore filter assay, whereas PMN maturation was determined with 31D8, a novel monoclonal antibody that binds to mature PMNs more avidly than immature PMNs and band forms.
  
  
  RESULTS
  In patients suffering blunt trauma, mean PMN chemotactic values were similar among children (44.6 +/- 2.3 microns) and adults (41.3 +/- 2.1 microns) and both were significantly less than among healthy adults (53.5 +/- 2.4 microns, P < .0005). PMN chemotactic values increased significantly in the 9 days after trauma for both children and adults (F = 13.8, df = 1, P < .0002). Mean PMN 31D8 binding among children with trauma (92.5 +/- 5.2) was significantly less than among healthy adults (117.6 +/- 5.4, P < .0009).
  
  
  CONCLUSIONS
  Impairment in PMN chemotaxis occurs in children after blunt trauma and is due in part to release of immature PMNs into the circulation.
authors.affiliation: Department of Pediatrics and Medicine, Hartford Hospital, CT 06102-5037.
authors.forename: P J
authors.initials: PJ
authors.lastname: Krause
authors.forename: C L
authors.initials: CL
authors.lastname: Woronick
authors.forename: G
authors.initials: G
authors.lastname: Burke
authors.forename: N
authors.initials: N
authors.lastname: Slover
authors.forename: C
authors.initials: C
authors.lastname: Kosciol
authors.forename: T
authors.initials: T
authors.lastname: Kelly
authors.forename: B
authors.initials: B
authors.lastname: Spivack
authors.forename: E G
authors.initials: EG
authors.lastname: Maderazo
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000911:Antibodies, Monoclonal; D016022:Case-Control Studies; D002634:Chemotaxis, Leukocyte; D002648:Child; D006801:Humans; D009504:Neutrophils / Q000276:immunology / Q000502:physiology; D014949:Wounds, Nonpenetrating / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal