A Mucor pusillus mutant defective in asparagine-linked glycosylation.
A Mucor pusillus mutant defective in asparagine-linked glycosylation was found in our stock cultures. This mutant, designated 1116, secreted aspartic proteinase (MPP) in a less-glycosylated form than that secreted by the wild-type strain. Analysis of enzyme susceptibility, lectin binding, and carbohydrate composition indicated that this mutant secreted three glycoforms of MPPs, one of which contained no carbohydrate; the other two had truncated asparagine-linked oligosaccharide chains such as Man0-1GlcNAc2. Further analysis using oligosaccharide processing inhibitors, such as castanospermine, 1-deoxynojirimycin and N-methyldeoxynojirimycin, suggested that MPPs in the mutant were glycosylated through a transfer of the truncated lipid-linked oligosaccharides, Man0-1GlcNAc2, to the MPP protein but not through an aberrant processing. In addition, genetic studies with forced primary heterokaryons indicated that the mutation in strain 1116 was recessive.
Murakami
K
K
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Aikawa
J
J
Wada
M
M
Horinouchi
S
S
Beppu
T
T
D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives / Q000494:pharmacology; D001216:Asparagine / Q000378:metabolism; D005816:Genetic Complementation Test; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors; D006031:Glycosylation; D007212:Indolizines / Q000494:pharmacology; D009089:Mucor / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D010434:Pepsin A / Q000235:genetics* / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D001216:Asparagine; D006026:Glycoside Hydrolases; D007212:Indolizines; D009844:Oligosaccharides; D010434:Pepsin A; C037806:castanospermine; D054836:Indolizidines; C033424:N-methyldeoxynojirimycin