Effect of the pSV2-neo plasmid on NIH 3T3 cell motion detected electrically.
Advantage was taken of DNA transfection techniques to investigate the effect of the pSV2-neo plasmid and its derivatives on recipient NIH 3T3 cell motion. Cell spreading and motion were followed by a newly developed electrical method to monitor cell morphology, referred to as electric cell-substrate impedance sensing. Using this method, we found that the eukaryotic-prokaryotic shuttle vector pSV2-neo had a strong effect on the recipient NIH 3T3 cell spreading and cell motion. However, two new neo plasmids, pSK-neo and pSP-neo, which were constructed by modifying the pSV2-neo plasmid, did not have a significant effect on the recipient cell activities. The results suggest that there may be some sequences in pSV2-neo which affect recipient cell behavior.
Pei
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Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590.
Keese
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Giaever
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Kurzawa
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Wilson
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D016475:3T3 Cells / Q000166:cytology* / Q000502:physiology; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D004594:Electrophysiology; D005822:Genetic Vectors; D051379:Mice; D015183:Restriction Mapping; D013329:Structure-Activity Relationship