Quantitative analysis of nifedipine in plasma by high-performance liquid chromatography.
A rapid, sensitive and specific high-performance liquid chromatographic assay for the quantification of nifedipine in human plasma was developed, satisfactorily validated and applied to samples of plasma from healthy volunteers. The sample pre-treatment incorporating protein denaturation by urea and ethyl acetate extraction compared favourably in terms of selectivity with previously published methods. The limit of quantitation of this reversed-phase LC method was 7.0 ng ml-1 for the analysis of 0.5 ml samples.
Thongnopnua
P
P
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Viwatwongsa
K
K
D000085:Acetates / Q000737:chemistry; D000284:Administration, Oral; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006207:Half-Life; D006801:Humans; D009543:Nifedipine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D014508:Urea / Q000737:chemistry
D000085:Acetates; C007650:ethyl acetate; D014508:Urea; D009543:Nifedipine