Postmortem chorionic villus sampling for cytogenetic evaluation of early fetal loss: a simple reliable method.
BACKGROUND
Early fetal losses associated with chromosome abnormalities produce most spontaneous abortions. It is not feasible to conduct cytogenetic studies with tissue culture postmortem because of tissue maceration and autolysis.


METHODS
A total of 30 specimens from early fetal losses were karyotyped for chromosomal study. Success rate of the culture method is evaluated and compared.


RESULTS
The abnormal karyotype rate was 33.3%. It was found that chorionic villus tissues had a higher culture rate than tests of fetal skin, amnion or amniocyte by early amniocentesis.


CONCLUSIONS
Postmortem chorionic villus sampling at the time of diagnosis of early fetal death appears to offer the best, most simple and reliable method for obtaining a cytogenetic result, and also a good method for training residents in the chorionic villus sampling technique.
Chen
H Y
HY
Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan, R.O.C.
Yang
T C
TC
Yah
L S
LS
Hsu
T Y
TY
Tsai
H D
HD
Chang
K S
KS
Hung
K S
KS
D000328:Adult; D000649:Amniocentesis; D002824:Chorionic Villi / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D005240:Feasibility Studies; D005260:Female; D005313:Fetal Death / Q000235:genetics* / Q000473:pathology; D005865:Gestational Age; D006801:Humans