A regulatory subunit of smooth muscle myosin bound phosphatase.
The relationship between two putative myosin-binding subunits of smooth muscle myosin phosphatase was investigated. A monoclonal antibody (MoAb) to the 58 kD component of smooth muscle myosin-bound phosphatase (MBP) cross-reacted with a 130 kD protein in extracts of fresh chicken gizzards. The MoAb in combination with protein A immunoprecipitated from gizzard extracts a complex of the 130 kD protein plus the 38 kD catalytic subunit of the type 1 delta protein phosphatase. It is proposed that the 130 kD component is a native subunit of MBP and that the 58 kD protein is its proteolytic degradation product. The distribution of the 130 kD component in chicken tissues was screened using the MoAb. An immunoreactive band of appropriate mass was detected in all tissues except liver and skeletal muscle. Higher concentrations of the 130 kD component were evident in the smooth muscle samples.
Okubo
S
S
1st Department of Internal Medicine, Mie University School of Medicine, Japan.
Ito
M
M
Takashiba
Y
Y
Ichikawa
K
K
Miyahara
M
M
Shimizu
H
H
Konishi
T
T
Shima
H
H
Nagao
M
M
Hartshorne
D J
DJ
D000818:Animals; D002645:Chickens; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005895:Gizzard, Avian; D015151:Immunoblotting; D046911:Macromolecular Substances; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000201:enzymology*; D043263:Myosin-Light-Chain Phosphatase; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity; D010749:Phosphoprotein Phosphatases / Q000302:isolation & purification / Q000378:metabolism*
D046911:Macromolecular Substances; D010749:Phosphoprotein Phosphatases; D043263:Myosin-Light-Chain Phosphatase; D009218:Myosins