Properties of phosphorylated glial fibrillary acidic protein in brain slices of guinea pig, mouse and rat.
1. Brain micro-slices from guinea pig, mouse and rat were incubated in Krebs-Ringer Na-HEPES buffered medium containing [32P]-phosphate and characterized by immunoblotting with polyclonal antibody to glial fibrillary acidic protein (GFAP). 2. GFAP presented small differences in two-dimensional electrophoretic mobility. 3. The phosphorylation of GFAP was dependent on Ca2+ in the incubation medium in adult animals. 4. Both the immunocontent and level of phosphorylation of GFAP were higher in hippocampus than in cerebral cortex of all three species.
Gonçalves
C A
CA
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Salbego
C G
CG
Rodnight
R
R
D000818:Animals; D002127:Calcium Isotopes; D002540:Cerebral Cortex / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D005904:Glial Fibrillary Acidic Protein / Q000737:chemistry* / Q000378:metabolism; D006168:Guinea Pigs; D006624:Hippocampus / Q000737:chemistry*; D051379:Mice; D010766:Phosphorylation; D051381:Rats
D002127:Calcium Isotopes; D005904:Glial Fibrillary Acidic Protein