Circulating polymorphonuclear leukocytes from patients with gram-negative bacteremia are not primed for enhanced production of leukotriene B4 or 5-hydroxyeicosatetraenoic acid.
The hypothesis was tested that polymorphonuclear leukocytes (PMNL) from patients with gram-negative bacteremia are primed to produce leukotriene B4 (LTB4) or 5-hydroxyeicosatetraenoic acid (5-HETE), in response to concentrations of calcium ionophore A23187, which are substimulatory for control PMNL. PMNL from 11 bacteremic patients and 8 healthy subjects (11 samples) produced similar quantities of LTB4, omega-oxidation products of LTB4, and 5-HETE after incubation with 0.3 and 0.5 microM A23187 for 5 min. At the detection threshold of 0.3 microM A23187, LTB4 was present in PMNL preparations from 9 of 11 patients and 7 of 11 control samples and 5-HETE from the same 9 patients and from 6 controls. There was no correlation between LTB4 or 5-HETE and plasma levels of endotoxin. In this group of patients, priming of PMNL by gram-negative bacteremia did not lead to enhanced production of LTB4, its omega-oxidation products, or 5-HETE when PMNL were challenged with low concentrations of A23187.
Sorrell
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TC
Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Australia.
Sztelma
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May
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D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016470:Bacteremia / Q000276:immunology*; D000001:Calcimycin / Q000494:pharmacology; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000276:immunology*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*
D004731:Endotoxins; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D000001:Calcimycin; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid