Assignment of the gene for porcine insulin-like growth factor 1 (IGF1) to chromosome 5 by linkage mapping.
Investigation of published sequence data from the porcine insulin-like growth factor 1 (IGF1) gene, resulted in the detection of a microsatellite in the first intron of the gene. Polymerase chain reaction (PCR) primers flanking the (CA)19 repeat were constructed. Polymorphism and Mendelian segregation were documented in a three-generation pedigree and allele frequencies were determined in 74 unrelated animals from four different breeds. Seven alleles were encountered. Linkage analysis was performed in a large pedigree established for gene mapping. Linkage between the IGF1 microsatellite and an anonymous microsatellite marker, S0005, was detected. Furthermore, IGF1 and S0005 was found to be linked to the porcine submaxillary gland mucin (MUC) gene, previously assigned to chromosome 5. The results presented here extend the linkage group on pig chromosome 5 and are in accordance with conserved synteny between human chromosome 12, cattle chromosome 5, mouse chromosome 10 and pig chromosome 5.
Winterø
A K
AK
Department of Animal Genetics, Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark.
Fredholm
M
M
Andersson
L
L
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000662:veterinary*; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics*
D017931:DNA Primers; D004276:DNA, Satellite; D007334:Insulin-Like Growth Factor I