DNA repair is less efficient in the nuclear matrix than in non-matrix nuclear fractions in the liver of rats treated with 2-aminofluorene.
The amount of DNA adducts and radioactive thymidine incorporation into DNA fractions attached and not attached to the nuclear matrix in the liver of rats treated with the carcinogen 2-aminofluorene (2-AF) were compared. The rate of [3H]thymidine incorporation was directly proportional to the amount of adducts in total hepatic DNA. Within the first 10 h after the carcinogen treatment, the level of adducts in the nuclear matrix DNA was higher than in the whole nuclei. The rate of [3H]thymidine incorporation into the nuclear matrix DNA was 5-30% lower than into DNA in whole nuclei at any time after 2-AF injection. We suggest that in rat liver cells, the 2-AF-induced DNA repair does not occur in close contact with the nuclear matrix.
Widlak
P
P
Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
Rzeszowska-Wolny
J
J
D000818:Animals; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D004260:DNA Repair; D005449:Fluorenes / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology; D008099:Liver / Q000187:drug effects*; D008297:Male; D015530:Nuclear Matrix / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D005449:Fluorenes; C012177:2-aminofluorene; D006918:Hydroxyurea