Detection of antibodies to Afipia species by the microagglutination test.
A microagglutination test with heat-killed, safranin-stained whole-cell antigen for detecting antibodies to Afipia species has been developed. An upper limit of the normal titer of antibodies to Afipia species was found to be > 10 by testing 430 sera. Therefore, a titer of 20 can be considered the lowest titer suggestive of infection. Overall, 5-7% of the sera were positive for Afipia felis, 3% were positive for Afipia broomeae and 11% were positive for Afipia clevelandensis. The frequency of sera positive for unnamed Afipia genospecies 1-3 was < 1%. The agglutinating antibodies belonged mainly to the IgM class.
Müller
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HE
Staatliches Medizinaluntersuchungsamt Braunschweig, Germany.
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis*; D002372:Cat-Scratch Disease / Q000175:diagnosis*; D005260:Female; D006090:Gram-Negative Bacteria / Q000276:immunology*; D006801:Humans; D008297:Male; D012698:Serologic Tests
D000907:Antibodies, Bacterial