Recognition of B and T cell epitopes by cattle immunized with a synthetic peptide containing the major immunogenic site of VP1 FMDV 01 Campos.
The precise location of B and T cell epitopes have been established in a peptide containing the major immunogenic site (residues 135-160) of FMDV strain 01 Campos (01C) VP1. The peptide (p135-160), administered free or conjugated to bovine serum albumin, induced complete protection in guinea pigs and a strong neutralizing antibody (NAb) response in cattle. Using a set of partially overlapping peptides it was shown that although several B cell epitopes were distributed along the p135-160, the residues responsible for the induction of NAb were restricted to the amino acids 135-144. In addition, bovines immunized with the p135-160 showed a strong proliferative response to this peptide but a very poor response against purified virus. Two T-epitopes were located in the 135-160 sequence: one on the region 135-144 and the other in the region 150-160. The immunodominance of these two T cell epitopes was confirmed in cattle immunized with inactivated virus vaccines.
Zamorano
P
P
Instituto de Virologia, CICV, INTA, Castelar, Buenos Aires, Argentina.
Wigdorovitz
A
A
Chaher
M T
MT
Fernandez
F M
FM
Carrillo
C
C
Marcovecchio
F E
FE
Sadir
A M
AM
Borca
M V
MV
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002417:Cattle; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control; D006168:Guinea Pigs; D016056:Immunodominant Epitopes / Q000032:analysis* / Q000276:immunology; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D036022:Capsid Proteins; D016056:Immunodominant Epitopes; C442501:VP1 protein, Foot-and-mouth disease virus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines