title: Promoting role of Clonorchis sinensis infection on induction of cholangiocarcinoma during two-step carcinogenesis.
abstract: Chronic Clonorchis sinensis (CS) infection is etiologically related to cholangiocarcinoma (CHCA) in human and animals. This study was carried out to clarify the role of CS infection on dimethylnitrosamine (DMN)-induced cholangiocarcinogenesis. Fifteen hamsters were administered with 15 ppm DMN for 4 weeks and one week later, the hamsters were infected with 15 metacercariae of CS (DMN-->CS group). The other 15 hamsters were infected with CS and after 5 weeks they were treated with the drug, praziquantel. Again one week later, the hamsters were administered with DMN (CS-->DMN group). The other 15 hamsters were administered with DMN and CS simultaneously (CS+DMN group). Histopathological examination of the livers showed CHCA with papillary or adenomatous hyperplasia of bile ductules in 3 of 15 hamsters of DMN-->CS group and in 11 of 15 hamsters of DMN+CS group. These results suggest that CS infection to hamsters may have a promoting effect on the development of CHCA.
authors.affiliation: Department of Parasitology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: H M
authors.initials: HM
authors.lastname: Yang
authors.forename: U B
authors.initials: UB
authors.lastname: Bak
authors.forename: H J
authors.initials: HJ
authors.lastname: Rim
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000737:chemistry / Q000209:etiology*; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma / Q000139:chemically induced / Q000209:etiology*; D003003:Clonorchiasis / Q000150:complications*; D003004:Clonorchis sinensis; D003043:Cocarcinogenesis; D006224:Cricetinae; D004128:Dimethylnitrosamine; D006801:Humans; D008099:Liver / Q000473:pathology; D008647:Mesocricetus
chemical_list: D004128:Dimethylnitrosamine