title: Experience with cloned mitochondrial DNA analysis to forensic practice.
abstract: The sequencing method of the D-loop region of cloned mitochondrial DNA (mtDNA) together with multilocus and single locus DNA probe patterns was applied to identification of the dismembered and putrefied remains of a pregnant woman. The multilocus DNA fingerprinting was useful to identify whether or not 4 dismembered remains were from the body of the same person, whereas the single locus DNA analysis was useful to decide that the suspect was the father of the fetus. On the other hand, the sequencing method of the D-loop region of cloned mtDNA after amplification by polymerase chain reaction (PCR) was particularly advantageous to highly degraded and contaminated DNA specimens.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Tsukamoto
authors.forename: W D
authors.initials: WD
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Oono
authors.forename: K
authors.initials: K
authors.lastname: Iwadate
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: T
authors.initials: T
authors.lastname: Takatori
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D015342:DNA Probes; D004272:DNA, Mitochondrial / Q000032:analysis*; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy
chemical_list: D015342:DNA Probes; D004272:DNA, Mitochondrial