Adsorption of aerosil on erythrocyte surface by flow cytometry measurements.
Light scattering properties of the erythrocytes in less than 0.01% aerosil suspension were measured. It was shown that light scattering properties of intact cells and cells associated with silica (aerosil) are different. It was also established that the aerosil particles are characterized by more intensive autofluorescence in the channel of green fluorescence than the erythrocytes. We propose that this observation allows a facile means of studying the interaction of purified silica and cell membranes. Hemolysis, as a result of interaction between the aerosil particles and the erythrocytes, was confirmed by photometric measurement of hemoglobin in the supernatant.
Gerashchenko
B I
BI
Department of Pathology, Medical College of Georgia, Augusta 30912-3605.
Gerashchenko
I I
II
Bogomaz
V I
VI
Pantazis
C G
CG
D000327:Adsorption; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000187:drug effects; D005434:Flow Cytometry; D005453:Fluorescence; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008027:Light; D012542:Scattering, Radiation; D012822:Silicon Dioxide / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties
D012822:Silicon Dioxide