[Antiviral activity of IgG subclasses of immunoglobulin preparations for intravenous use].
Preparations of human immunoglobulins for intravenous use (IVIG) should contain proper percentage of IgG subclasses, responding to physiological preparations with preserved activity of antibodies. The study was aimed at establishment of activity against measles, CMV and HSV viruses in individual subclasses of Bioglobulin (Biomed, Warszawa) and a comparison with preparation Polygam (Baxter, USA). Indirect immunofluorescence test was used. The results revealed presence of antiviral activity mostly in subclass IgG1 and also in IgG3, which is in keeping with results obtained by other authors. We have found an anti-HSV activity in a subclass IgG2 and IgG4 in IVIG preparations which was not yet described in the literature; it is confirmed, however, in investigations with application of sera of HSV-positive persons. These discrepancies may be caused by different methods of detection. Viral antigens in ELISA and IF tests may differ among themselves by content of individual epitopes. Basing on obtained results it can be stated that Polish preparation Bioglobulin (in which for the first time antiviral activity was determined in subclasses) is comparable with Polygam.
Litwińska
B
B
Zakład Wirusologii PZH w Warszawie.
Bucholc
B
B
Biesiadecka
A
A
Kańtoch
M
M
D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology; D004338:Drug Combinations; D006801:Humans; D007070:Immunoglobulin A / Q000008:administration & dosage / Q000276:immunology*; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000276:immunology*; D007075:Immunoglobulin M / Q000008:administration & dosage / Q000276:immunology*; D007275:Injections, Intravenous; D008457:Measles / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology
D000914:Antibodies, Viral; D004338:Drug Combinations; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C077495:immunoglobulin A, immunoglobulin G, immunoglobulin M drug combination
Aktywność przeciwwirusowa w podklasach IgG preparatów immunoglobulin do stosowania dozylnego.