[Structure of integrated oncogens E1A and E1B in a malignant line of rat SH2 fibroblasts, transformed by monkey adenovirus SA7 (C8) DNA].
In this investigation the primary structure of E1A and E1B regions of SA7 (C8) simian adenovirus integrated in malignant SH2 rat cell line was studied. Southern blotting revealed at least two copies of the SA7 oncogene integrated in the SH2 genome. PCR analysis of E1A and E1B regions showed heteroduplex structures, proving the different structure of the integrated copies. The heteroduplex molecules with different electrophoretic mobility were separated, and chains corresponding to different copies were sequenced according to the modified Sanger method. We found that two copies differ mainly in microsatellite regions, in E1A between positions 894-902 (GCG)3/(GCG)4, in E1B between positions 2037-2048 (GCA)3/(GCA)4. It is necessary to stress that all deviations found belong to the coding regions of the SA7 oncogene.
Surin
V L
VL
Sats
N V
NV
Tagiev
A F
AF
D017500:Adenovirus E1A Proteins / Q000235:genetics*; D017501:Adenovirus E1B Proteins / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004279:DNA, Viral; D005347:Fibroblasts / Q000378:metabolism; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D051381:Rats
D017500:Adenovirus E1A Proteins; D017501:Adenovirus E1B Proteins; D004279:DNA, Viral
Struktura integrirovannykh onkogenov D1A i E1B v zlokachestvennoĭ linii fibroblastov krysy SH2, transformirovannykh DNK adenovirusa obez'ian SA7 (C8).