Feedback repression of polyamine transport is mediated by antizyme in mammalian tissue-culture cells.
Antizyme, a spermidine-induced protein that binds and stimulates ornithine decarboxylase degradation, is now shown also to mediate the rapid feedback inhibition of polyamine uptake into mammalian cells. Using a cell line (HZ7) transfected with truncated antizyme cDNA, and mutant ornithine decarboxylase cell lines, we demonstrate that this newly discovered action of antizyme is distinct from its role in modulating polyamine biosynthesis.
Mitchell
J L
JL
Department of Biological Sciences, Northern Illinois University, DeKalb 60115.
Judd
G G
GG
Bareyal-Leyser
A
A
Ling
S Y
SY
D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
D009838:Oligodeoxyribonucleotides; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D011506:Proteins; C031017:ornithine decarboxylase antizyme