[Studies of humoral immunity induced by proteins of the cell wall of Hafnia alvei].
It was found that cell wall proteins (CWP) of Hafnia alvei applied intraperitoneally to mice are inducing, beside previously detected cellular immunity, also humoral immunity. Applying ELISA immunoenzymatic test in which a conjugate enabling demonstration of IgG antibodies was applied, it was found that CWP of Hafnia alvei induce appearance of specific antibodies in a high titer, but their presence in mouse serum is short and independent from applied doses of CWP. Humoral immunity induced by CWP can be transferred passively to nonimmunized animals by application of high level immune serum, which protected fully the animals against infection with homological strains of Hafnia alvei even in dilution 1:100. Investigated serum did not exhibit protective activity against infection with heterological strains of Hafnia alvei.
Witkowska
D
D
Instytut Immunologii i Terapii Doświadczalnej PAN, Wrocławiu.
Czarny
A
A
Mulczyk
M
M
D000818:Animals; D000917:Antibody Formation; D002473:Cell Wall / Q000276:immunology*; D004755:Enterobacteriaceae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000008:administration & dosage / Q000276:immunology; D007111:Immunity, Cellular; D051379:Mice; D013045:Species Specificity
D007106:Immune Sera
Badania nad odpornościa humoralna indukowana białkami ściany komórkowej Hafnia alvei.