Extracellular matrix regulates and L-ascorbic acid 2-phosphate further modulates morphology, proliferation, and collagen synthesis of perisinusoidal stellate cells.
Morphology, proliferation, and collagen synthesis of perisinusoidal stellate cells (fat-storing cells, lipocytes, Ito cells) varied according to the nature of the substratum. On a basement membrane gel, the stellate cells formed a mesh-like structure and proliferated slowly. On non-coated polystyrene and type I collagen-coated culture dishes, the cells spread well and extended cellular processes. The cell proliferation and collagen synthesis were more prominent on culture dishes coated with type I collagen than on polystyrene dishes. On each extracellular substrate, proliferation and collagen synthesis were stimulated by a long-acting vitamin C derivative, L-ascorbic acid 2-phosphate. These results indicate that morphology, proliferation, and collagen metabolism of the stellate cells are regulated by extracellular matrix and modulated further by L-ascorbic acid 2-phosphate.
Senoo
H
H
Department of Anatomy, School of Medicine, Tokyo, Japan.
Hata
R
R
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D001485:Basement Membrane / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D003094:Collagen / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011137:Polystyrenes; D051381:Rats; D017208:Rats, Wistar; D012176:Retinoids / Q000378:metabolism
D011137:Polystyrenes; D012176:Retinoids; C011669:ascorbate-2-phosphate; D003094:Collagen; D004247:DNA; D001205:Ascorbic Acid