Cloning, structure, cellular localization, and possible function of the tumor suppressor gene lethal(3)malignant blood neoplasm-1 of Drosophila melanogaster.
The tumor suppressor gene, lethal(3)malignant blood neoplasm-1+, of Drosophila melanogaster is required for the differentiation of the phagocytic blood-cell type, the plasmatocyte. In the homozygously mutated state it causes the malignant transformation of these blood cells. We present here the cloning, sequencing, structure, and expression of the l(3)mbn-1+ gene during development. The cloned gene was identified by germ-line transformation, generation of revertants, and the detection of the corresponding mRNA in blood cells and other tissues. Homologies of the G-S-rich C-terminus of the putative MBN83 protein to human cytokeratins K1, K10, and mouse loricrin were found. The structure and possible function of the wild-type l(3)mbn-1+ gene are discussed.
Konrad
L
L
Institut für Genetik, Johannes Gutenberg Universität, Mainz, Federal Republic of Germany.
Becker
G
G
Schmidt
A
A
Klöckner
T
T
Kaufer-Stillger
G
G
Dreschers
S
S
Edström
J E
JE
Gateff
E
E
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001773:Blood Cells / Q000473:pathology*; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004331:Drosophila melanogaster / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis
D018076:DNA, Complementary; D012333:RNA, Messenger