title: One-megabase yeast artificial chromosome and 400-kilobase cosmid-phage contigs containing the von Hippel-Lindau tumor suppressor and Ca(2+)-transporting adenosine triphosphatase isoform 2 genes.
abstract: We have isolated and ordered yeast artificial chromosomes (YACs) and cosmids surrounding the von Hippel-Lindau (VHL) tumor suppressor and plasma membrane Ca(2+)-transporting ATPase isoform 2 (PMCA-2) genes on chromosome 3p25-26. The YAC contig consists of six YACs and covers a region of 1 megabase. A cosmid-phage contig around VHL and PMCA-2 genes (400 kilobases) was established and integrated into the YAC map. Using these clones, we generated an EcoRI map of the 400-kilobase region. PMCA-2 and VHL complementary DNA were positioned entirely within the cosmid-phage contig as well as two polymorphic markers (D3S601 and D3S1317). This physical map of the cloned region will allow a detailed analysis of both the PMCA-2 and VHL genes. Some of the genomic clones may be useful for isolation of the full-length VHL complementary DNA.
authors.affiliation: Laboratory of Immunobiology, Research and Development Center, Maryland 21702-1201.
authors.forename: I
authors.initials: I
authors.lastname: Kuzmin
authors.forename: T
authors.initials: T
authors.lastname: Stackhouse
authors.forename: F
authors.initials: F
authors.lastname: Latif
authors.forename: F M
authors.initials: FM
authors.lastname: Duh
authors.forename: L
authors.initials: L
authors.lastname: Geil
authors.forename: J
authors.initials: J
authors.lastname: Gnarra
authors.forename: M
authors.initials: M
authors.lastname: Yao
authors.forename: M L
authors.initials: ML
authors.lastname: Orcutt
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Tory
mesh_terms: D001483:Base Sequence; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000737:chemistry; D002893:Chromosomes, Human, Pair 3; D003360:Cosmids / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D006623:von Hippel-Lindau Disease / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D000252:Calcium-Transporting ATPases