Diazepam binding inhibitor fragment 33-50 (octadecaneuropeptide) immunoreactivity in the cerebellar cortex is restricted to glial cells.
The distribution of octadecaneuropeptide (ODN)-like immunoreactivity (LI) and its relationship to gamma-aminobutyric acid (GABA)-LI were investigated in the cerebellar cortex of adult rats with electron microscopy. At the electron microscopic level, ODN-LI was found exclusively in glial cells. In addition to Bergmann glia and its processes, cerebellar astrocytes were also labelled, encapsulating unlabelled neuronal elements of the cerebellum. These ODN-LI glial processes were observed in close apposition to synaptic junctions, but immunoreactivity could not be found in the synaptic cleft or in association with neuronal membranes. Since GABA-LI is always associated with neuronal elements, the colocalization of GABA- and ODN-LI could not be confirmed in the cerebellar cortex. Our results do not support the assumption that ODN is a neuron-specific processing product of diazepam binding inhibitor.
Vidnyánszky
Z
Z
First Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary.
Görcs
T J
TJ
Hámori
J
J
D000818:Animals; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism*; D003975:Diazepam / Q000037:antagonists & inhibitors*; D026261:Diazepam Binding Inhibitor; D006098:Granulocytes / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009457:Neuroglia / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism* / Q000494:pharmacology*; D010446:Peptide Fragments; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution
D026261:Diazepam Binding Inhibitor; D009479:Neuropeptides; D010446:Peptide Fragments; C067529:diazepam binding inhibitor (33-50); D003975:Diazepam