Characterization of a functional angiotensin II receptor in Xenopus laevis heart.
High-affinity (104 +/- 18 pmol/l) and high-density (204 +/- 25 fmol/mg) angiotensin II (AII) binding sites have been identified in Xenopus laevis heart membranes. Competition binding of [125I]Sar1,Ile8 angiotensin (SIA) to these receptors by peptide analogs selective for the mammalian AII receptor subtypes AT1 and AT2 suggested that the amphibian AII binding sites were more closely related to the AT1 receptor subtype. Also in common with AT1 receptors, dithiothreitol and GTP gamma S inhibited [125I]SIA binding to Xenopus heart receptors, exhibiting IC50 values of 600 and 0.95 mumol/l, respectively. In addition, Xenopus oocytes injected with Xenopus heart mRNA were capable of mobilizing calcium when exposed to AII, demonstrating that Xenopus AII receptors are functionally linked to a second-messenger system similar to that coupled to mammalian AT1 receptors. However, in contrast to both AT1 and AT2 receptor subtypes, nonpeptide antagonists DUP 753 and SK&F 108566 (AT1 receptor selective) and PD123319 (AT2 selective) did not bind the Xenopus AII receptors, thus establishing that the amphibian receptors were pharmacologically unique. Together, these results demonstrate that Xenopus heart AII receptors are functionally similar to mammalian AT1 receptors but are pharmacologically distinct from both AT1 and AT2 receptors.
Aiyar
N
N
Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pa 19406-0939.
Baker
E
E
Pullen
M
M
Nuthulaganti
P
P
Bergsma
D J
DJ
Kumar
C
C
Nambi
P
P
D015059:1-Sarcosine-8-Isoleucine Angiotensin II / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011869:Radioligand Assay; D011945:Receptors, Angiotensin / Q000378:metabolism*; D015290:Second Messenger Systems / Q000502:physiology; D014982:Xenopus laevis / Q000378:metabolism*
D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D015059:1-Sarcosine-8-Isoleucine Angiotensin II; D019204:GTP-Binding Proteins; D002118:Calcium; D004229:Dithiothreitol