Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway.
Genetic data from Drosophila have suggested a functional relationship between the novel cytoplasmic protein encoded by the deltex locus and the transmembrane receptor encoded by Notch. We have demonstrated a direct interaction between these proteins from expression studies conducted in cultured cells, in yeast, and in the imaginal wing disc. deltex binds specifically to the Notch ankyrin repeats, a region that is crucial for Notch signaling and that constitutes the most conserved domain among Notch family members. In addition, we present a new Notch allele, Nsu42c, that is associated with a missense mutation within the fifth ankyrin repeat. In addition to representing a new class of viable Notch allele, this mutation behaves similarly to mutations of deltex and further implicates the ankyrin repeats in Notch function.
Diederich
R J
RJ
Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06536-0812.
Matsuno
K
K
Hing
H
H
Artavanis-Tsakonas
S
S
D000483:Alleles; D000818:Animals; D017089:Ankyrin Repeat / Q000502:physiology*; D001483:Base Sequence; D002478:Cells, Cultured; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins; D007301:Insect Hormones / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D051880:Receptors, Notch; D012441:Saccharomyces cerevisiae / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D014921:Wings, Animal / Q000196:embryology
D029721:Drosophila Proteins; D007301:Insect Hormones; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch