A simple and rapid HPLC assay for quantitating cyclic AMP accumulation in intact cell preparations.
A high performance liquid chromatographic method is described for measuring adenosine 3',5'-cyclic monophosphate (cyclic AMP) accumulation in intact or whole-cell preparations using ultraviolet (UV) detection. This quantitative technique is rapid, employing a simple step for the extraction of cyclic AMP from tissues.
Elnatan
A
A
School of Pharmacology, Victorian College of Pharmacy, Monash University, Parkville, Australia.
Mitchelson
F
F
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003106:Colon / Q000737:chemistry / Q000378:metabolism; D000242:Cyclic AMP / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics; D005260:Female; D006168:Guinea Pigs; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013268:Stimulation, Chemical
D000242:Cyclic AMP; D007545:Isoproterenol