Differential induction of Pax genes by NGF and BDNF in cerebellar primary cultures.
The Pax genes encode sequence-specific DNA binding transcription factors that are expressed in embryonic development of the nervous system. Primary neuronal cell cultures derived from the cerebellar cortex of embryonic day 14, newborn and 7-d old mice, were used to investigate the cell-type specific expression patterns of three members of the murine paired box containing gene family (Pax gene family), in vitro. Cell types which express Pax-2, Pax-3, and Pax-6 RNA in primary cultures correspond to those found in regions of the cerebellum which show RNA signals in sections of the developing mouse brain. To find mechanisms regulating Pax gene expression during cerebellar development, the differential regulation of Pax-2, Pax-3, and Pax-6 by NGF and BDNF, two structurally related neurotrophins, was studied in such primary cultures. Pax-2 and Pax-6 RNA increased slightly by 1 h and remained elevated throughout a 24-h treatment with BDNF and NGF. Pax-3 RNA was not detected in newborn cultures, but underwent a rapid (1 h) and transient (2 h) induction upon treatment with either BDNF or NGF. No response was seen with EGF or FGF. Cycloheximide treatment amplified Pax-3 induction and prolonged the signal. Thus, Pax-3 induction resembles that of the immediate-early gene c-fos, which transduces growth factor signals during the development of particular neuronal/glial cell types. The changes in Pax expression were inductive rather than trophic.
Kioussi
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Abteilung für Molekular Zellbiologie, Max-Planck Institut für Biophysikalische Chemie, Göttingen, Germany.
Gruss
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D000818:Animals; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D005136:Eye Proteins; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009414:Nerve Growth Factors / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D051762:PAX2 Transcription Factor; D000072041:PAX3 Transcription Factor; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D010641:Phenotype; D016133:Polymerase Chain Reaction; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics*
D019208:Brain-Derived Neurotrophic Factor; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D051762:PAX2 Transcription Factor; D000072041:PAX3 Transcription Factor; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C497319:Pax2 protein, mouse; C000605732:Pax6 protein, mouse; D017475:Receptors, Nerve Growth Factor; D012097:Repressor Proteins; D014157:Transcription Factors; C068686:Pax3 protein, mouse; D004247:DNA