[Identification of enteropathogenic strains of E. coli (EPEC) by application of the latex. II. A trial of applying the test in diagnostic examinations].
The study was aimed at evaluation of diagnostic usefulness, prepared by own method, of a set of latex reagents for identification of enteropathogenic strains of E. coli (EPEC). EPEC strains in feces of 334 children with diarrhoea were searched by application at the same time of two methods: used routinely to this time slide agglutination test with commercial OK sera for enteropathogenic serotypes of E. coli and by a latex test with application of warmed culture of investigated strain in peptone water and polystyrene latex coated with immune globulins for O antigens of EPEC serotypes. By application of both methods EPEC strains were found in 50 (15.04%) children, by a latex test in 94 (28.1%) and by agglutination with OK sera in 67 (20.1%) of tested patients. Conformity of results obtained by both tests amounted to 82%. The latex test was found more useful for identification of EPEC strains because of simplicity of performance, higher sensitiveness and better repeatability of results, when compared with agglutination test with live E. coli and OK sera.
Jagielski
M
M
Zakład Bakteriologii PZH, Warszawie.
Szych
J
J
Kałuzewski
S
S
Rastawicki
W
W
Brzezińska
K
K
Kulec
W
W
Lauterbach
B
B
D002648:Child; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007841:Latex Fixation Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping; D013045:Species Specificity
Identyfikacja enteropatogennych szczepów E. coli (EPEC) przy zastosowaniu testu lateksowego. II. Próba wykorzystania testu w badaniach diagnostycznych.