title: Euro-Collins flush perfusion in human lung preservation--ultrastructural studies of the preservation quality of the contralateral donor lung in clinical single lung transplantation.
abstract: The quality of pulmonary preservation after Euro-Collins flush perfusion was assessed by means of morphometric analysis based on transmission electron microscopy. In five patients undergoing single lung transplantation, the contralateral donor lung that could not be matched for another recipient was studied by means of light microscopy and transmission electron microscopy. While one of the donor lungs was transplanted, the contralateral lung was fixed by airway instillation at the same time and subsequently processed for microscopic examination. Although light microscopy showed an excellent quality of organ preservation, transmission electron microscopy revealed the presence of fine to medium alterations at the level of the air-blood-barrier. In the five contralateral donor lungs, different degrees of the cellular alterations were recorded by morphometric analysis, which correlated with differences observed in the early postoperative course of the patients receiving the other lung, respectively. Although in four of five patients the clinical course showed no complications and extubation was performed within 36 hours after the operation, one patient required artificial ventilation over a period of 10 days because of impaired oxygenation of the transplanted lung. In this patient, morphometric analysis of the air-blood-barrier showed a significantly (p < 0.02) smaller surface fraction of normal type 1 pneumocytes, a significantly (p < 0.05) smaller volume density of the capillary endothelial cells, and a significantly (p < 0.01) higher volume density of type 2 pneumocytes. The alterations of the alveolar epithelium have to be interpreted as a result of influences occurring during the donor's medical history rather than being an effect of preservation and/or ischemia.
authors.affiliation: Division of Electron Microscopy, Georg August University, Göttingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Fehrenbach
authors.forename: S W
authors.initials: SW
authors.lastname: Hirt
authors.forename: T
authors.initials: T
authors.lastname: Wahlers
authors.forename: P A
authors.initials: PA
authors.lastname: Schnabel
authors.forename: A
authors.initials: A
authors.lastname: Haverich
authors.forename: J
authors.initials: J
authors.lastname: Richter
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015824:Blood-Air Barrier; D002196:Capillaries / Q000473:pathology; D015925:Cryopreservation; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D006982:Hypertonic Solutions / Q000008:administration & dosage / Q000627:therapeutic use*; D008168:Lung / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure*; D016040:Lung Transplantation / Q000473:pathology / Q000502:physiology; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009926:Organ Preservation; D010100:Oxygen / Q000097:blood; D010477:Perfusion; D011650:Pulmonary Alveoli / Q000098:blood supply / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000601:surgery
chemical_list: C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D010100:Oxygen