[Complementary-addressed photomodification of nucleic acids by arylazide and perfluoroarylazide oligonucleotide derivatives. III. Oligonucleotide reagents with a photoactive group at the end or inside the chain; tandem reagents].
Photomodification of target oligonucleotides with reagents bearing p-azidotetrafluorobenzamide group at various positions of the oligonucleotide address was investigated. The photoactive group was attached to the 5'- or 3'-terminal phosphate or at the C5-position of a deoxyuridine residue at the 5'-end or inside the oligonucleotide chain. The reagents with the internal photoactive group modified the target with 50-55% efficiency (fraction of covalent adducts reagent-target), whereas the derivatives with a terminal reactive group were more effective (70%). The main point of the modification was the guanosine residue of the target which located near to the photoactive group and was not involved into the duplex formation. Tandems of reagents which are complementary to neighbouring sites of the target modify predominantly the same guanosine residue, with up to 80% extent.
Levina
A S
AS
Tabatadze
D R
DR
Zarytova
V F
VF
Dobrikov
M I
MI
Gorn
V N
VN
D001386:Azides / Q000737:chemistry*; D001483:Base Sequence; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry
D001386:Azides; D007202:Indicators and Reagents; D009696:Nucleic Acids; D009841:Oligonucleotides
Komplementarno-adresovannaia fotomodifikatsiia nukleinovykh kislot arilazidnymi i perftorarilazidnym proizvodnymi oligonukleotidov. III. Oligonukleotidnye reagenty s fotoaktivnoĭ gruppoĭ na kontse ili vnutri tsepi; tandemy reagentov.