Evaluation of perilla ketone-induced unilateral lung injury using external gamma scanning.
We used a modified external gamma scanning technique to quantitate right and left lung permeability changes to iodinated sheep albumin before and after perilla ketone (PK)-mediated unilateral lung injury in seven anesthetized sheep. Three portable gamma scintillation probes containing 2-in. NaI crystals detected radioactivities of 51Cr-labeled red blood cells and 125I-labeled albumin over the right and left lungs and blood, respectively. Radioactivities were monitored for 1 h before and 3 h after infusion of 25 mg/kg PK into a single lung. Calculation of normalized slope index (NSI) (Roselli and Riddle, J. Appl. Physiol. 67: 2343-2350, 1989) over the 30-min interval before PK and over the 60- to 90-min interval after PK for each lung revealed a four- to five-fold NSI increase in lungs receiving PK (0.00237 +/- 0.00065 to 0.0109 +/- 0.0016 min-1) and no increase in contralateral control lungs (0.00214 +/- 0.00065 to 0.00201 +/- 0.00032 min-1). Observed changes in NSI were consistent with postmortem evaluations of each lung. Lungs receiving PK had significantly higher wet-to-dry lung weight ratios and extravascular lung water volumes than contralateral control lungs. Measured bloodless wet-to-dry lung weight ratios were 5.68 +/- 0.39 and 3.27 +/- 0.27 (P < 0.05) for PK and control lungs, respectively.
Abernathy
V J
VJ
Department of Biomedical Engineering, School of Engineering, Vanderbilt University, Nashville, Tennessee 37235.
Pou
N A
NA
Parker
R E
RE
Roselli
R J
RJ
D000818:Animals; D001784:Blood Gas Analysis; D001810:Blood Volume / Q000502:physiology; D002199:Capillary Permeability / Q000502:physiology; D002860:Chromium Radioisotopes; D004727:Endothelium / Q000502:physiology; D015902:Gamma Cameras; D007958:Leukocyte Count; D008171:Lung Diseases / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000503:physiopathology; D008208:Lymphatic System / Q000502:physiology; D039821:Monoterpenes; D009929:Organ Size / Q000502:physiology; D011652:Pulmonary Circulation / Q000502:physiology; D011877:Radionuclide Imaging; D012711:Serum Albumin, Radio-Iodinated; D012756:Sheep; D013729:Terpenes; D014118:Toxins, Biological
D002860:Chromium Radioisotopes; D039821:Monoterpenes; D012711:Serum Albumin, Radio-Iodinated; D013729:Terpenes; D014118:Toxins, Biological; C015426:perilla ketone