Organotropic formation and disappearance of 8-hydroxydeoxyguanosine in the kidney of Sprague-Dawley rats exposed to adriamycin and KBrO3.
A form of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG), was comparatively determined for 48 h in the kidney and liver isolated from Sprague-Dawley rats i.p. treated with Adriamycin; potassium bromate (KBrO3), hydroquinone and vitamin A. HPLC-ECD analysis system showed that Adriamycin and KBrO3, renal carcinogens, induced higher levels of 8-OHdG in the target organ of kidney (12-13.8 residues/10(4) dG(deoxyguanosine)) compared to those in the liver (3.4-3.8 residues/10(4) dG) and showed highly persistent levels (8 residues, 10(4) dG) in the kidney. The data suggest that the organotropic persistence of 8-OHdG may provide a useful marker for identifying target organ systems in oxidative chemical carcinogenesis and screening free radical-generating carcinogens.
Cho
D H
DH
Department of Toxicology, National Institute of Safety Research, Seoul, Korea.
Hong
J T
JT
Chin
K
K
Cho
T S
TS
Lee
B M
BM
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001959:Bromates / Q000009:adverse effects*; D002273:Carcinogens; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D004317:Doxorubicin / Q000009:adverse effects*; D004790:Enzyme Induction; D005260:Female; D006873:Hydroquinones / Q000009:adverse effects; D007668:Kidney / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014801:Vitamin A / Q000009:adverse effects
D001959:Bromates; D002273:Carcinogens; D006873:Hydroquinones; C019536:potassium bromate; D014801:Vitamin A; D004317:Doxorubicin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C031927:hydroquinone