Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming.
This paper describes a general procedure for the two-step purification of recombinant proteins as antibody-antigen complexes in which there is no uncomplexed antibody or antigen. In this way, immune complexes containing the p17, p27, vpr and vpx proteins of simian immunodeficiency virus (SIV) have been purified. Antibody-antigen complexes are more immunogenic than antigen when administered either alone or with alum. The significance of the work is that this general method could be modified for the manufacture of immune complexes for incorporation into multivalent vaccines.
Randall
R E
RE
Young
D
D
Hanke
T
T
Szawlowski
P
P
Botting
C
C
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology* / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins