title: Growth modulation and proteoglycan turnover in cultured mesangial cells.
abstract: Proliferation of mesangial cells is a common feature of renal disease, and conditioned media from glomerular epithelial and endothelial cells have been found to contain heparin-like molecules that suppress proliferation of rat mesangial cells (RMC). We have partially characterized the glycosaminoglycans that are labeled with 35SO4(2-) by RMC in culture at early passage and examined their ability to inhibit mitogenic stimulation of the cells. Four chondroitin/dermatan sulfate proteoglycans (CS/DSPG) were identified, the largest and smallest of which (Kd of 0.04 and 0.26 on Superose 6) were retained in the cell layer while the other two (Kd = 0.17 and 0.22) were secreted into the medium. Heparan sulfate proteoglycans (HSPG) with Kd values of 0.09, 0.13, and 0.39 were minor components of the cell layer, while a single heparan sulfate (Kd = 0.17) was recovered from the medium. After 16 h of labeling in serum-free medium, about 60% of macromolecular 35S was cell-associated and 40% was in the medium. Cell-associated label consisted of 7% CS/DSPG, 9% HSPG, and 84% free glycosaminoglycan chains (mostly CS/DS), whereas the medium contained 52% CS/DSPG, 17% HSPG, and approximately equal amounts of free HS and CS/DS chains. Bovine lung heparin (1 microgram/ml) decreased by 45% the incorporation of [3H]-thymidine into DNA after release of serum-starved RMC from growth arrest. Heparin acted prior to the G1/S interface; arrest of the cells in early S phase with aphidicolin abrogated the heparin response. The endogenous HSPGs had a slight antimitogenic effect on the RMC, but heparan sulfate chains from both the medium and cell layer had a potent effect. On an equivalent mass basis, only the free glycosaminoglycan chains were more potent than heparin in this regard, decreasing thymidine incorporation by over 90% when present at 1 microgram/ml. These results demonstrate that heparan sulfate glycosaminoglycans derived from mesangial proteoglycans are potential negative autocrine growth regulators. Proteoglycan metabolism releases these soluble heparan sulfate chains, determining the level of this activity.
authors.affiliation: Department of Clinical Biochemistry, University of Toronto, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Wang
authors.forename: M Y
authors.initials: MY
authors.lastname: Fan
authors.forename: D M
authors.initials: DM
authors.lastname: Templeton
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D005920:Glomerular Mesangium / Q000166:cytology* / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D008297:Male; D008934:Mitogens / Q000037:antagonists & inhibitors; D011509:Proteoglycans / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D003470:Culture Media; D008934:Mitogens; D011509:Proteoglycans; D006493:Heparin