Pharmacological demonstration of a barrier for protease, peptides and acetylcholine in the endometrium of the rat.
Contractions of the rat uterus in response to trypsin, kallikrein, bradykinin, angiotensin II, oxytocin and acetylcholine, were abolished when an inside-out preparation was used. Sensitivity to Ba++, however, was preserved. In preparations in which the endometrium was mechanically removed, all above cited agonists elicited contractions. By treating the uterus with both collagenase and hyaluronidase, acetylcholine was able to induce a contraction when applied to the endometrium side of the uterus. The results show that a barrier for protease, peptides and acetylcholine is present in the mucosa of the rat uterus.
Andrade
S P
SP
Department of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Feitosa
M H
MH
Guimarães
J A
JA
Rogana
E
E
Oliveira
G M
GM
Ferreira
M A
MA
Beraldo
W T
WT
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D003094:Collagen / Q000502:physiology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects*; D010450:Endopeptidases / Q000494:pharmacology*; D005260:Female; D006025:Glycosaminoglycans / Q000502:physiology; D066298:In Vitro Techniques; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014590:Uterine Contraction / Q000502:physiology
D006025:Glycosaminoglycans; D010455:Peptides; D003094:Collagen; D010450:Endopeptidases; D000109:Acetylcholine