title: Role of different subtypes of P2 purinoceptor on cytosolic Ca2+ levels in rat aortic smooth muscle.
abstract: The role of different subtypes of P2 purinoceptors on cytosolic Ca2+ level ([Ca2+]i) was examined in vascular smooth muscle of rat aorta. alpha beta-Methylene-ATP (P2X agonist), 2-methylthio-ATP (P2Y agonist), UTP and ATP gamma S (P2U agonists), and ATP (nonselective P2 agonist) induced a transient increase followed by a small sustained increase in [Ca2+]i in a concentration dependent manner. Among these agonists, alpha beta-methylene-ATP was the most potent. In the absence of extracellular Ca2+ (with 0.5 mM EGTA), ATP, UTP and ATP gamma S induced a transient increase in [Ca2+]i whereas alpha beta-methylene-ATP and 2-methylthio-ATP were ineffective. ATP gamma S showed the highest potency in Ca(2+)-free solution. After desensitization of P2X purinoceptor, ATP, UTP and ATP gamma S induced a rapid increase in [Ca2+]i followed by a sustained increase while alpha beta-methylene-ATP and 2-methylthio-ATP were ineffective. These results suggest that Ca2+ release from the intracellular Ca2+ store is mediated by P2U purinoceptor whereas Ca2+ influx is mediated by both P2X and P2U purinoceptors in the rat aortic smooth muscle.
authors.affiliation: Department of Veterinary Pharmacology, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kitajima
authors.forename: H
authors.initials: H
authors.lastname: Ozaki
authors.forename: H
authors.initials: H
authors.lastname: Karaki
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000378:metabolism*; D013873:Thionucleotides / Q000494:pharmacology; D014544:Uridine Triphosphate / Q000494:pharmacology
chemical_list: D018048:Receptors, Purinergic P2; D013873:Thionucleotides; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D002118:Calcium; D014544:Uridine Triphosphate; C008501:2-methylthio-ATP