title: Molecular cloning and functional expression of gicerin, a novel cell adhesion molecule that binds to neurite outgrowth factor.
abstract: Gicerin is an integral membrane glycoprotein of about 82 kd that is transiently expressed in the developing CNS. Gicerin was first identified as a binding protein for neurite outgrowth factor (NOF), a member of the laminin family of extracellular matrix proteins. By isolating and sequencing a gicerin cDNA, we have found that this protein is a novel member of the immunoglobulin superfamily. The deduced protein (584 amino acids) consists of five immunoglobulin-like loop structures in an extracellular domain, a single transmembrane region, and a short cytoplasmic tail. Cells transfected stably with gicerin cDNA adhered to NOF and aggregated with each other, indicating that gicerin exhibits both heterophilic and homophilic adhesion activities.
authors.affiliation: Department of Pharmacology, Osaka University School of Medicine, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Taira
authors.forename: N
authors.initials: N
authors.lastname: Takaha
authors.forename: H
authors.initials: H
authors.lastname: Taniura
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: N
authors.initials: N
authors.lastname: Miki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D051929:CD146 Antigen; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000378:metabolism*; D014162:Transfection
chemical_list: D030161:Avian Proteins; D051929:CD146 Antigen; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C497903:Mcam protein, mouse; D009414:Nerve Growth Factors; C086935:gicerin protein, Gallus gallus