Avian myeloblastosis virus core-bound 7 S DNA, highly bent minute structures with sequence-directed curvature.
Structural properties and length distribution profile of 7 S avian myeloblastosis virus (AMV) DNA were studied by means of electron microscopy using two different techniques. This DNA represents mostly double strands, the single strands being in minority. We have shown directly that this DNA forms a bent structure typical of the majority of molecules. These bends are sensitive to the distamycin treatment which stretches most of the bent molecules. Some amount (up to 30%) of circular DNA molecules was detected also in DNA preparations, the nature and the size of which are reminiscent of electron microscopic data on microbubbles of replicating DNA. No specific AMV DNA structural features were found using osmium-tetroxide treatment. The basic size of AMV DNA was estimated to be approximately 150 bp, but its multimers were also detected. Their presence and significance is discussed.
Korb
J
J
Institute of Molecular Genetics, Academy of Sciences, Prague, Czech Republic.
Stokrová
J
J
Ríman
J
J
Sulová
A
A
D000818:Animals; D001353:Avian Leukosis / Q000382:microbiology; D009189:Avian Myeloblastosis Virus / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D002645:Chickens; D004270:DNA, Circular / Q000737:chemistry / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000648:ultrastructure; D004279:DNA, Viral / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure*; D004214:Distamycins / Q000494:pharmacology; D008854:Microscopy, Electron; D015394:Molecular Structure; D009690:Nucleic Acid Conformation / Q000187:drug effects; D012192:Retroviridae Infections / Q000382:microbiology
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004214:Distamycins