[C-reactive protein modulates neutrophil adhesiveness and biocidal activity].
The effect of C-reactive protein (CRP) on the adhesive and biocidal activity (AA and BA) of human neutrophils (HN) in vitro was studied. AA was evaluated by binding peroxidase-labeled soy bean and peanut lectins in the enzymatic color reaction and the subsequent comparison of the results with the know method of staining HN with crystal violet. The criterion of BA was myeloperoxidase activity (MA) evaluated spectrophotometrically by H2O2 degradation in the presence of O-phenylenediamine. The proposed modification of the test for AA is more sensitive than the known ones. Serum pentameric CRP (pCRP) enhanced AA in a dose-dependent manner (with the maximum activity at concentrations of 25-50 mg/ml, which corresponded to its serum levels at the beginning of inflammation). MA was enhanced by pCRP, but not by CRP degraded to monomers, this effect being abolished by actinomycin D. Possible mechanisms of the action of CRP are discussed.
Polevshchikov
A V
AV
Nazarov
P G
PG
Berestovaia
L K
LK
D002097:C-Reactive Protein / Q000302:isolation & purification / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D009195:Peroxidase / Q000097:blood / Q000187:drug effects; D012709:Serum Albumin / Q000494:pharmacology
D012709:Serum Albumin; D003609:Dactinomycin; D002097:C-Reactive Protein; D009195:Peroxidase
C-reaktivnyĭ belok moduliruet adgezivnost' i biotsidnost' neĭtrofilov.