Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity.
To characterize the function of the Ly-6A antigen in T cell activation, antisense Ly-6 RNA was expressed in a stably transfected antigen-specific T cell clone. Reduced Ly-6A expression results in inhibition of responses to antigen, anti-TCR (anti-T cell receptor) crosslinking and concanavalin A plus recombinant interleukin 1 and causes impairment of in vitro fyn tyrosine kinase activity. More substantial reduction of Ly-6A results in reduction of TCR expression. Analysis of mRNA species indicates that the reduction is specific for the TCR beta chain. These data demonstrate that Ly-6A may regulate TCR expression and may be involved in early events of T cell activation via regulation of fyn tyrosine kinase activity.
Lee
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SK
Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520-8011.
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B
Maher
S E
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Bothwell
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D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002999:Clone Cells; D003001:Cloning, Molecular; D017931:DNA Primers; D005434:Flow Cytometry; D007438:Introns; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051076:Proto-Oncogene Proteins c-fyn; D016372:RNA, Antisense / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*; D014162:Transfection
D000950:Antigens, Ly; D017931:DNA Primers; D011518:Proto-Oncogene Proteins; D016372:RNA, Antisense; D011948:Receptors, Antigen, T-Cell; D011505:Protein-Tyrosine Kinases; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn