Enhancement of macrophage candidacidal activity by interferon-gamma.
In previous studies, we have reported that opsonized candida species are ingested by monocytes and monocyte-derived macrophages (MDM), but uptake of unopsonized candida is mediated only by MDM, primarily through the mannose receptor (MR). This study examines the effects of recombinant IFN-gamma on the uptake and killing of unopsonized C. albicans by MDM. We report here that MDM treated with IFN-gamma developed an increase in their capacity to ingest and kill unopsonized C. albicans and to release O2- upon stimulation with candida. Mannan (0.1 to 5 mg/ml) inhibited uptake of candida in a dose-dependent manner, but glucan (5 mg/ml) did not. These data suggest that mannose receptors may be involved in the increased phagocytosis and killing of unopsonized candida by human macrophages treated with IFN-gamma.
Maródi
L
L
Department of Pediatrics, University School of Medicine, Debrecen, Hungary.
Johnston
R B
RB
D000328:Adult; D002176:Candida albicans / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005936:Glucans / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008351:Mannans / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011994:Recombinant Proteins; D013481:Superoxides / Q000378:metabolism
D005936:Glucans; D008351:Mannans; D011994:Recombinant Proteins; D013481:Superoxides; D007371:Interferon-gamma