Effects of Pseudostellaria heterophylla on proliferation and differentiation of murine bone marrow cells.
The incorporation of tritiated thymidine into the DNA of murine bone marrow cells could be stimulated by fraction PH-I Ba separated from the roots of Pseudostellaria heterophylla in a dose-dependent manner in vitro. Moreover, PH-I Ba could induce the differentiation of murine bone marrow cells from pluripotent haemopoietic stem cells into macrophages-like cells in vitro. Autocrine or paracrine stimulation of granulocyte-macrophage colony stimulating factor was likely to underly the induction of differentiation. Therefore, PH-I Ba was proved to be an immunostimulating agent for mouse marrow hematopoiesis and was found to be a polysaccharide. The sugar components were analyzed by Gas Liquid Chromatography of their alditol acetates.
Wong
C K
CK
Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories.
Leung
K N
KN
Fung
K P
KP
Choy
Y M
YM
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002849:Chromatography, Gas; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D051379:Mice
D004365:Drugs, Chinese Herbal; D016178:Granulocyte-Macrophage Colony-Stimulating Factor