title: Practical use of polymerase chain reaction for the diagnosis of steroid induced tuberculous lymphadenitis.
abstract: Tuberculous lymphadenitis that had developed after treatment with systemic prednisolone was diagnosed using the polymerase chain reaction (PCR) combined with dot-blot hybridization. Although the patient had no history of tuberculosis or suggestive radiological findings, a DNA fragment specific for Mycobacterium tuberculosis complex strains was amplified from a paraffin-embedded biopsy specimen of a cervical swelling, which was compatible with tuberculous lymphadenitis on haematoxylin-eosin (HE) and Ziehl-Neelsen staining. This report demonstrates the practical use of PCR for diagnosis of extra-pulmonary tuberculosis in a case difficult to diagnose conventionally.
authors.affiliation: Department of Dermatology, Yokohama City University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sugita
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: K
authors.initials: K
authors.lastname: Okuda
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
mesh_terms: D001483:Base Sequence; D001706:Biopsy; D004247:DNA; D003882:Dermatomyositis / Q000188:drug therapy; D005260:Female; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D011239:Prednisolone / Q000009:adverse effects*; D014388:Tuberculosis, Lymph Node / Q000139:chemically induced* / Q000473:pathology*
chemical_list: D004247:DNA; D011239:Prednisolone