Suppression of tumorigenicity in human teratocarcinoma cell line PA-1 by introduction of chromosome 4.
Teratocarcinomas are tumors that develop spontaneously in the gonads and usually contain a rapidly dividing, undifferentiated stem cell population. Immature ovarian teratocarcinomas are highly malignant with only 30-60% of patients surviving for 2 years after diagnosis. We have used microcell fusion to introduce individually tagged normal human chromosomes into the PA-1 human teratocarcinoma cell line. Introduction of human chromosome 4 caused a cell morphology in culture and suppressed PA-1 tumorigenicity in nude mice, whereas addition of portions of either chromosome 7 or 12 had no effect on the cell phenotype. The PA-1 cell line regained its tumorigenicity when the tagged chromosome 4 was lost under negative selection. We conclude that there is a putative tumor suppressor gene on human chromosome 4 whose expression interferes with the tumorigenicity of PA-1 cells.
McGowan-Jordan
I J
IJ
Department of Biochemistry, Faculty of Medicine, University of Ottawa, Ontario, Canada.
Speevak
M D
MD
Blakey
D
D
Chevrette
M
M
D000818:Animals; D002648:Child; D002894:Chromosomes, Human, Pair 4; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D010641:Phenotype; D018243:Teratocarcinoma / Q000235:genetics* / Q000473:pathology; D014162:Transfection / Q000235:genetics / Q000379:methods*; D014407:Tumor Cells, Cultured