Heterogeneity of T cells specific for a particular peptide/HLA-DQ complex.
Whether T cells specific for a particular peptide/HLA-DQ complex are restricted with respect to TCR usage has not been fully established. TCR usage of T cells specific for a peptide presented by a given HLA-DQ molecule has not been studied before. We therefore sequenced the TCR genes of five different TCCs derived from the same donor, which were specific for a p21 ras-derived synthetic peptide presented by the HLA-DQ(alpha 1*0102,beta*0602) (DQ6) molecule. We found that these T cells which recognized the same peptide/HLA-DQ complex used highly diverse TCRs. However, dose-response experiments using various truncations of the p21 ras-derived peptide revealed that the peptide fine specificities of the five TCCs were not completely identical. This may explain the heterogeneity in TCR usage.
Spurkland
A
A
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Gedde-Dahl
T
T
Hansen
T
T
Vartdal
F
F
Gaudernack
G
G
Thorsby
E
E
D000595:Amino Acid Sequence; D000938:Antigen-Presenting Cells / Q000276:immunology; D001483:Base Sequence; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D015689:Oncogene Protein p21(ras) / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D006683:HLA-DQ Antigens; D009841:Oligonucleotides; D010455:Peptides; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015689:Oncogene Protein p21(ras)