title: Characterization of restriction endonuclease AjoI from Acinetobacter johnsonii.
abstract: A new type II restriction endonuclease, named AjoI, was detected in Acinetobacter johnsonii. The enzyme AjoI, an isoschizomer of PstI, recognized the hexanucleotide sequence [5'-CTGCA/G-3'], with a cleavage site generating fragments of DNA with protruding cohesive 3' termini.
authors.affiliation: Department of Microbiology, Technical University of Gdańsk, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Nowak
authors.forename: J
authors.initials: J
authors.lastname: Kur
authors.forename: E
authors.initials: E
authors.lastname: Gospodarek
authors.forename: K
authors.initials: K
authors.lastname: Bielawski
mesh_terms: D000150:Acinetobacter / Q000201:enzymology*; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific