Preliminary report on cell encapsulation in a hydrogel made of a biocompatible material, AN69, for the development of a bioartificial pancreas.
The occurrence of an inflammatory reaction represents the major obstacle to the development of any implantable system including micro and macroencapsulation. The purpose of this study was to describe an encapsulation method for cells using a membrane made of AN69, a copolymer of acrylonitrile which is considered as a reference in biocompatibility in the field of haemodialysis. The hydrogel of AN69 was obtained after a coagulation step at room temperature followed by a solvent/non-solvent (water) exchange phase. Microcapsules were obtained by co-extrusion of AN69 collodion and saline (with or without cells). The function of encapsulated cells was assessed in vitro, demonstrating cell survival after the microencapsulation procedure. These preliminary data are consistent with the potential interest for the development of the microencapsulation procedure aimed at realising a bioartificial pancreas.
Honiger
J
J
INSERM Bioengineering Laboratories, Hôpital Saint-Antoine, Paris, France.
Darquy
S
S
Reach
G
G
Muscat
E
E
Thomas
M
M
Collier
C
C
D000180:Acrylic Resins; D000181:Acrylonitrile / Q000031:analogs & derivatives*; D000818:Animals; D001187:Artificial Organs; D001672:Biocompatible Materials; D004339:Drug Compounding; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D008567:Membranes, Artificial; D010179:Pancreas; D011092:Polyethylene Glycols; D051381:Rats
D000180:Acrylic Resins; D001672:Biocompatible Materials; D007328:Insulin; D008567:Membranes, Artificial; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; C016892:AN-69; D000181:Acrylonitrile