Intravenous Helicobacter pylori induces low levels of TNF-alpha and IL-1 alpha in a murine model.
A murine model for testing cytokine production stimulated by Helicobacter pylori is described. H. pylori induced significantly lower levels of TNF-alpha and IL-1 alpha compared to Escherichia coli or Pseudomonas aeruginosa when injected intravenously. The mean TNF-alpha concentration in serum during 6 h after stimulation with H. pylori was 0.2 ng/ml, whereas E. coli induced 4.7 ng/ml and P. aeruginosa 6.0 ng/ml. This was not explained by rapid elimination of H. pylori as bacteria were present for at least 3 h in the blood. The difference in cytokine induction may be a reflection of the bacteria's different biological qualities. E. coli and P. aeruginosa are both capable of causing systemic disease, whereas H. pylori causes only a local, often low grade, inflammation in the gastric mucosa.
Wadström
T
T
Department of Medical Microbiology, University of Lund, Sweden.
Rydberg
J
J
Rozalska
B
B
Lelwala-Guruge
J
J
D000818:Animals; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000737:chemistry; D004927:Escherichia coli Infections / Q000097:blood; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000097:blood; D016480:Helicobacter pylori / Q000737:chemistry*; D007275:Injections, Intravenous; D007375:Interleukin-1 / Q000097:blood*; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011552:Pseudomonas Infections / Q000097:blood; D011550:Pseudomonas aeruginosa / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha