title: Effect of in vitro and in vivo migration of bovine neutrophils on binding and expression of Fc receptors for IgG2 and IgM.
abstract: Binding of endogenous and exogenous homologous IgG2 and IgM to bovine neutrophils before and after in vitro migration through micropore filters, and in vivo migration through mammary tissues after intramammary injection of endotoxin was evaluated by use of flow cytometry. Immunoglobulin binding to neutrophils at 4 and 37 C was also evaluated. Before and after in vitro migration, neutrophils with endogenously bound IgG2 and IgM averaged 1 and 2% and 23 and 7%, respectively. Before and after in vivo migration, IgG2 and IgM binding averaged 1 and 7% and 26 and 15%, respectively. Before and after in vitro migration, binding of purified IgG2 and IgM averaged 75 and 67% and 8 and 24%, respectively. Before and after in vivo migration, percentage of neutrophils binding purified IgG2 and IgM averaged 92 and 98% and 54 and 70%, respectively. When serum was used as a source of exogenous immunoglobulins, binding of total IgG after in vitro migration increased from 5% to 28% and of IgM from 4% to 20%. After in vivo migration, binding increased from 21% to 47% and from 24% to 56%, respectively. Exogenous binding of IgG2 at 4 and 37 C averaged 75 and 84%, and binding of IgM averaged 8% at either temperature. Endogenous IgG2 was unaffected by temperature, however, binding of IgM decreased from 23% at 4 C to 2% at 37 C. These data indicate that endogenous binding was higher for IgM before migration than after migration, in vitro and in vivo. Furthermore, migration in vivo through cellular matrices induced receptor upregulation for IgG and IgM.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Sciences, University of Maryland, College Park 20742.
authors.forename: M
authors.initials: M
authors.lastname: Worku
authors.forename: M J
authors.initials: MJ
authors.lastname: Paape
authors.forename: R
authors.initials: R
authors.lastname: Filep
authors.forename: R H
authors.initials: RH
authors.lastname: Miller
mesh_terms: D000818:Animals; D002417:Cattle; D002634:Chemotaxis, Leukocyte; D004731:Endotoxins; D004926:Escherichia coli; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification / Q000378:metabolism*; D007075:Immunoglobulin M / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D007958:Leukocyte Count; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D011961:Receptors, Fc / Q000096:biosynthesis / Q000378:metabolism*; D017452:Receptors, IgG / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D004731:Endotoxins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011961:Receptors, Fc; D017452:Receptors, IgG; C035311:immunoglobulin M receptor