The antibacterial activity of a siderophore. 3. The activity of deferoxamine in vitro and its influence on the effect of antibiotics against Escherichia coli, Proteus mirabilis and coagulase-negative staphylococci.
The in vitro activity of deferoxamine (DFO) both per se and in combination with the reductant ascorbic acid (AA) was determined against 10 E. coli strains, 5 P. mirabilis strains, and 10 coagulase-negative staphylococci. In terms of interaction, the influence of DFO on the activities of cephalothin and gentamicin was furthermore investigated against the same panel of strains employing a macrobroth dilution technique and killing-curve kinetics. The MICs of cephalothin and gentamicin were lowered for one half of the strains. Moreover, DFO prolonged the generation times of logarithmic growth phase considerably, especially when the reductant AA was present. The interactions between DFO or DFO+AA and subinhibitory concentrations of antibiotics were established by the application of growth constants, and resulted in synergy for 15 out of 25 strains with cephalothin and 9 out of 25 strains with gentamicin.
Hartzen
S H
SH
Department of Antibiotics, Statens Seruminstitut, Copenhagen, Denmark.
Frimodt-Møller
N
N
Thomsen
V F
VF
D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001205:Ascorbic Acid / Q000494:pharmacology; D002512:Cephalothin / Q000633:toxicity; D003676:Deferoxamine / Q000494:pharmacology*; D004347:Drug Interactions; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D005839:Gentamicins / Q000633:toxicity; D006801:Humans; D008826:Microbial Sensitivity Tests; D011513:Proteus mirabilis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D013210:Staphylococcus / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D014556:Urine / Q000382:microbiology
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D003676:Deferoxamine; D001205:Ascorbic Acid; D002512:Cephalothin