Characterization of restriction endonuclease AjoI from Acinetobacter johnsonii.
A new type II restriction endonuclease, named AjoI, was detected in Acinetobacter johnsonii. The enzyme AjoI, an isoschizomer of PstI, recognized the hexanucleotide sequence [5'-CTGCA/G-3'], with a cleavage site generating fragments of DNA with protruding cohesive 3' termini.
Nowak
A
A
Department of Microbiology, Technical University of Gdańsk, Poland.
Kur
J
J
Gospodarek
E
E
Bielawski
K
K
D000150:Acinetobacter / Q000201:enzymology*; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific