Radioprotective efficiency of a combination of hydroxytryptophan and AET on mouse bone marrow micronuclei.
Radioprotective effectiveness of a combination of HT and AET was evaluated by radiation induced micronucleus screening in mouse bone marrow. Swiss albino mice were injected with 2-Aminoethylisothiouronium bromide hydrobromide (AET)+5-hydroxy-L-tryptophan (HT) 30 minutes prior to a single whole body exposure of 8 and 12 Gy Co60 gamma rays. Animals were sacrificed at different intervals viz., 24 hours, 40 hours, 72 hours and 7 days after irradiation. Slides of femur marrow were screened thoroughly for cells with micronuclei. Marked decreases in the frequency of micronuclei in irradiated mice receiving radioprotector suggested that the combination has rendered protection to bone marrow cells against 8 Gy and 12 Gy whole body gamma irradiation. Micronucleated cells count is not altered by administration of the combination alone.
Gupta
M L
ML
Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
Ghose
A
A
D006916:5-Hydroxytryptophan / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects*; D005260:Female; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D014916:Whole-Body Irradiation; D000338:beta-Aminoethyl Isothiourea / Q000008:administration & dosage / Q000494:pharmacology*
D011837:Radiation-Protective Agents; D000338:beta-Aminoethyl Isothiourea; D006916:5-Hydroxytryptophan