Human factor B. Complete cDNA sequence of the BF*S allele.
The gene of human complement factor B (BF) is located within the class III region of the major histocompatibility complex. The knowledge of the coding sequence of the BF gene rests on a set of partial sequence studies reported by various sources, and full-length sequences ascribed to specific alleles of this polymorphic complement component have not yet been published. Now, we have isolated and sequenced a collection of cDNA clones derived from BF*S, the major BF allele. We present an uninterrupted, allele-specific sequence of the entire coding region and the 3' untranslated segment of the cDNA. Extensive comparison of this and previously available sequence data was carried out, and a number of base substitutions were observed in relation to some of the earlier sequences. The possibility that these differences arise from polymorphism in the BF gene is discussed.
Mejía
J E
JE
Laboratory for Research in Immunology, Faculty of Medicine, Louis Pasteur University, Strasbourg, France.
Jahn
I
I
de la Salle
H
H
Hauptmann
G
G
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D011415:Complement Factor B / Q000235:genetics*; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D006801:Humans; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D015342:DNA Probes; D004247:DNA; D011415:Complement Factor B