Elutriation of T-lymphoblastic cells and cell cycle analysis of fractions.
Elutriation of human lymphoblastic cells has to be performed at a flow rate of 13 ml/min at a temperature of 10 degrees C to retain a high cell viability. Cell cycle analysis of elutriated cells on the basis of their DNA contents is inaccurate. The combined detection of BrdUrd incorporated into the DNA and analysis of the DNA content offers an alternative. After elutriation, fractions of cells with increased percentages of either G1 or S phase cells can be selected on the basis of the median cell size as established by a coulter counter channelyzer.
Van Den Berg
A A
AA
Academic Medical Center, Department of Pediatrics, Amsterdam, The Netherlands.
Van Lenthe
H
H
Kipp
J B
JB
De Korte
D
D
Van Gennip
A H
AH
D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002458:Cell Fractionation / Q000379:methods; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002498:Centrifugation; D004247:DNA / Q000032:analysis; D006801:Humans; D013601:T-Lymphocytes / Q000166:cytology*
D004247:DNA