title: Acute effects of hindlimb unweighting on satellite cells of growing skeletal muscle.
abstract: The proliferative behavior of satellite cells in growing rat soleus and extensor digitorum longus muscles was examined at short periods after initiation of hindlimb unweighting. Mitotic activity of satellite cells in both muscles decreased below weight-bearing control levels within 24 h of initiation of hindlimb unweighting. This satellite cell response was > or = 48 h before any atrophic morphological changes that take place in the muscles. Suppression of mitotic activity was most severe in the soleus muscle where continuous infusion of label demonstrated that virtually all mitotic activity was abolished between 3 and 5 days. The results of this study suggest that satellite cell mitotic activity is a sensitive indicator of primary atrophic changes occurring in growing myofibers and may be a predictor of future morphological changes.
authors.affiliation: Department of Anatomy, University of Wisconsin, Madison 53706.
authors.forename: E
authors.initials: E
authors.lastname: Schultz
authors.forename: K C
authors.initials: KC
authors.lastname: Darr
authors.forename: A
authors.initials: A
authors.lastname: Macius
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D006614:Hindlimb / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008938:Mitosis; D024510:Muscle Development; D009132:Muscles / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344; D014893:Weightlessness / Q000009:adverse effects*
chemical_list: D001973:Bromodeoxyuridine