A sensitive fluorogenic enzyme linked immunosorbent assay for the detection of Vipera russelli venom.
A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA) has been adapted for the detection of Vipera russelli venom. The assay sensitivity was observed to be 0.1 pg/ml (1 x 10(-13) g/ml). Venoms from snakes of the Vipera group exhibited a high degree of cross-reactivity when tested with the antibody raised against V. russelli venom. With the exception of venom from Naja naja, all the tested venoms from unrelated families also showed cross-reactivity. This procedure is useful for detecting snake venom or its components in biological samples.
Bhatti
A R
AR
Defence Research Establishment Suffield, Department of National Defence, Medicine Hat, Alberta, Canada.
Wong
J P
JP
Siddiqui
Y M
YM
Siddiqui
S
S
D000818:Animals; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D014757:Viper Venoms / Q000032:analysis* / Q000276:immunology / Q000633:toxicity
D014757:Viper Venoms