Acetylcholinesterase and Na+,K(+)-ATPase activities in different regions of rat brain during insulin-induced hypoglycemia.
The activities of acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7), responsible for hydrolysis of acetylcholine and Na+,K(+)-ATPase (Mg(2+)-dependent ATP phosphohydrolase, EC 3.6.1.3), which plays a crucial role in neurotransmission, were determined in four brain regions after 1, 2, and 3 h of insulin administration. Significant decrease in the acetylcholinesterase and Na+,K(+)-ATPase activities was observed in the soluble and total particulate fractions from cerebral hemispheres, cerebellum, brain stem, and diencephalon + basal ganglia after 1, 2, and 3 h of insulin-induced hypoglycemia. Blood glucose level decreased significantly after 1 h of insulin administration and remained at low level for 2 h thereafter, whereas, the protein content in different subcellular fractions from four brain regions did not show any significant change under this physiological stress.
Kaur
G
G
Department of Zoology, Guru Nanak Dev University, Amritsar, India.
Arora
S K
SK
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001479:Basal Ganglia / Q000201:enzymology; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D001933:Brain Stem / Q000201:enzymology; D002531:Cerebellum / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004027:Diencephalon / Q000201:enzymology; D007003:Hypoglycemia / Q000139:chemically induced / Q000201:enzymology*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology
D007328:Insulin; D000110:Acetylcholinesterase; D000254:Sodium-Potassium-Exchanging ATPase