Characterization of lactoferrin binding to the MAC-T bovine mammary epithelial cell line using a biotin-avidin technique.
1. A non-radioisotopic method utilizing a biotin-avidin approach was used to characterize lactoferrin binding to the clonal MAC-T bovine mammary epithelial cell line. 2. Binding of lactoferrin to MAC-T cells and isolated membranes was specific and saturable. 3. Unlabeled lactoferrin competed for and displaced biotin-labeled lactoferrin from binding sites on mammary epithelial cells. In contrast, unlabeled transferrin did not compete. 4. Scatchard analysis of lactoferrin binding to MAC-T cell crude membranes was nonlinear, revealing two classes of binding sites with association constants (Ka) of 2.36 x 10(7) and 3.36 x 10(6) M-1. 5. Binding of lactoferrin to MAC-T cells may be associated with the initial events which result in decreased MAC-T cell proliferation.
Rejman
J J
JJ
Department of Animal Science, University of Tennessee, Knoxville 37901-1071.
Turner
J D
JD
Oliver
S P
SP
D000818:Animals; D001360:Avidin; D001710:Biotin; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D007781:Lactoferrin / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding
D001360:Avidin; D001710:Biotin; D007781:Lactoferrin