In vitro studies on chemoprotective effect of Purnark against benzo(a)pyrene-induced chromosomal damage in human lymphocytes.
Human lymphocytes were used as an assay system to test chemopreventive activity of natural products. Purnark, a mixture of extracts of turmeric, betel leaf and catechu, was tested for its chemoprotective activity against BP induced DNA damage. Sister chromatid exchange and micronuclei were used as markers to assess the protective activity of Purnark. Purnark gave 50-60% protection against BP induced SCEs and micronuclei. Purnark at 100 micrograms dose did not show any genotoxicity.
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Swami Prakashananda Ayurvedic Research Centre, Bombay, India.
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D000293:Adolescent; D000328:Adult; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002877:Chromosomes, Human / Q000187:drug effects* / Q000648:ultrastructure; D003474:Curcumin / Q000494:pharmacology*; D004249:DNA Damage; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D015162:Micronucleus Tests; D012854:Sister Chromatid Exchange / Q000187:drug effects
D016588:Anticarcinogenic Agents; C087280:purnark; D001564:Benzo(a)pyrene; D003474:Curcumin