[Quantitative aspects of osmotic opening of the blood-brain barrier in experimental hyperbilirubinemia].
The presence of a number of interesting factors in kernicteric newborns with low serum bilirubin levels has lead us to study one important aspect, the reversible osmotic opening of the blood-brain barrier. Initially, an experimental hyperbilirubinemia was developed by the infusion of a crystalline bilirubin solution. Next, an increased serum osmolality state was generated by the injection of two different levels of urea (348.9 +/- 12.4 and 388.9 +/- 11 mOsm/kg). The bilirubin binding reserve capacity was not affected; however, we found a significant elevation in bilirubin concentrations in the homogenized brains of the hyperosmolality groups (2.92 +/- 0.54 and 3.4 +/- 0.43 mg/gr versus control of 2.43 +/- 0.63 mg/gr, F = 8.43, p < 0.01). Cerebral125 albumin was increased to similar proportions in those groups submitted to hyperosmolality.
Domínguez Ortega
F
F
Servicio de Neonatología, Hospital Materno Infantil, Las Palmas.
Martín Zarza
M
M
Ormazábal Ramos
J C
JC
Rodríguez Luis
J C
JC
Doménech Martínez
E
E
D000418:Albumins / Q000032:analysis; D000818:Animals; D001663:Bilirubin / Q000032:analysis / Q000097:blood; D001812:Blood-Brain Barrier; D001923:Brain Chemistry; D006932:Hyperbilirubinemia / Q000139:chemically induced* / Q000150:complications; D007565:Jaundice / Q000139:chemically induced / Q000209:etiology; D007647:Kernicterus / Q000209:etiology; D009994:Osmolar Concentration; D051381:Rats; D017207:Rats, Sprague-Dawley
D000418:Albumins; D001663:Bilirubin
Aspectos cuantitativos de la apertura osmótica de la barrera hematoencefálica en hiperbilirrubinemia experimental.