title: Mapping DNA polymorphisms using PCR primers derived from the sequence of an avian CR1 element.
abstract: Primers complementary to the chicken middle repetitive sequence element CR1 were used to generate and simultaneously map polymorphic polymerase chain reaction products (CR1-PCR) with various chicken DNAs as templates. Ten primers were prepared using the sequence of a single CR1 element as a guide. These 10 primers generated 23 polymorphic CR1-PCR products. The average number of polymorphic CR1-PCR products generated using single primers (1.1 per primer) was significantly higher than the average number observed using combinations of two primers (0.3 per primer combination). The polymorphic CR1-PCR products were mapped in a subset of a reference backcross population designed for the genetic linkage analysis of the chicken. Nineteen of the polymorphic CR1-PCR products identified were assigned to 13 of 19 linkage groups characterized thus far in this population; three have yet to be linked to a specific map location. One of the CR1-PCR markers mapped to the chicken Z chromosome. There was no evidence for a significant clustering of CR1-PCR markers within the map, even at the site of the CR1 element whose sequence was used for primer design.
authors.affiliation: Department of Microbiology, Michigan State University, East Lansing 48824.
authors.forename: I
authors.initials: I
authors.lastname: Levin
authors.forename: L B
authors.initials: LB
authors.lastname: Crittenden
authors.forename: J B
authors.initials: JB
authors.lastname: Dodgson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002874:Chromosome Mapping; D017931:DNA Primers; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D017931:DNA Primers