[Interrelationship between the chain length of poly-L-lysine and the degree of protection of polyribonucleotide interferon inducers from human blood nucleases].
The action of the human total blood serum on polynucleotide interferon inducers, larifan and ridostin (natural double-stranded RNAs) and poly(I).poly(C) (a double-stranded complex of synthetic polyribonucleotides) used both in the free state and in the state shielded with poly-L-lysine was studied. The rate of the accumulation of the acid soluble products was compared with the residual interferon-inducing activity in mice. All the unshielded inducers were shown to completely loose their activity after a 4-hour contact with the serum. The protective activity of poly-L-lysine increased in parallel with the increase of its molecular weight and was maximal for the preparation with the molecular weight of 12300 +/- 1000 Da. Differences in the structure of the inducers and the mechanism of their biosynthesis and degradation must be taken into account.
Surzhik
M A
MA
Duks
A E
AE
Diatlova
N G
NG
Glazunov
E A
EA
Feldmane
G Ia
GIa
Timkovskiĭ
A L
AL
D006801:Humans; D007369:Interferon Inducers / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D008970:Molecular Weight; D009930:Organic Chemicals; D011107:Polylysine / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000494:pharmacology*; D012331:RNA, Fungal / Q000737:chemistry / Q000494:pharmacology*; D012260:Ribonucleases / Q000097:blood*
D007369:Interferon Inducers; D009930:Organic Chemicals; D012330:RNA, Double-Stranded; D012331:RNA, Fungal; C076741:ridostin; C066530:larifan; D011107:Polylysine; D012260:Ribonucleases
Vzaimosviaz' mezhdu dlinoĭ tsepi ékraniruiushchego poli-L-lizina i stepen'iu zashchity poliribonukleotidnykh induktorob interferona ot nukleas krovi cheloveka.