title: Purification of staphylococcal enterotoxin E.
abstract: Staphylococcal enterotoxin E has been purified by a combination of Red-A ligand chromatography and Mono-S (cation-exchange) chromatography. Commencing with 30 litres of fermenter-grown culture the method has been used to purify 17 mg of toxin, giving a purification yield of around 40%. The purity of the toxin was over 98% when analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, but demonstrated three isoelectric forms by isoelectric focusing, with pl values of 7.6, 7.4, and 7.0. This method adds to the versatility of the Red-A matrix system which can also be used for the purification of staphylococcal enterotoxins A, B, and C2.
authors.affiliation: Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltshire, United Kingdom.
authors.forename: R D
authors.initials: RD
authors.lastname: Brehm
authors.forename: H S
authors.initials: HS
authors.lastname: Tranter
mesh_terms: D004768:Enterotoxins / Q000302:isolation & purification*; D007526:Isoelectric Point; D013211:Staphylococcus aureus / Q000737:chemistry / Q000472:pathogenicity*
chemical_list: D004768:Enterotoxins; C049463:enterotoxin E, Staphylococcal