Assessment of lipid peroxidation associated with lung damage induced by oxidative stress. In vivo and in vitro studies.
The lung thiobarbituric acid-reactive substances (TBA-RS) content and the amount of ethane exhaled, two potential markers of the lipid peroxidation process, were measured in rats following intratracheal administration of chemicals stimulating the production of free radicals, i.e. paraquat, phorbol myristate acetate and ferrous ions. Five hours after treatment, autopsy revealed gross pulmonary damage but the lung TBA-RS and the ethane exhalation were not different from control animals. On the contrary, a large increase in ethane production was observed 2 hr after intraperitoneal administration of the hepatotoxic carbon tetrachloride. In vitro, incubation of lung and liver homogenates from control rats with ferrous iron led to the development of a lipid peroxidation process in both tissues but the accumulation of TBA-RS and ethane was much lower with homogenates from lung as compared to liver tissue. Those results suggest that the lung may be more resistant than the liver to the initiation and/or propagation of a lipid peroxidation process. The possibility that others markers than ethane and TBA-RS are more appropriate to detect this process in the lung must also be considered.
Schweich
M D
MD
Industrial Toxicology and Occupational Medicine Unit, School of Medicine, Catholic University of Louvain, Brussels, Belgium.
Lison
D
D
Lauwerys
R
R
D000818:Animals; D004980:Ethane / Q000032:analysis; D005296:Ferrous Compounds / Q000633:toxicity; D005609:Free Radicals; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D010269:Paraquat / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D005296:Ferrous Compounds; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D004980:Ethane; D013755:Tetradecanoylphorbol Acetate; D010269:Paraquat