[Dot immunobinding assay for detection of three types of rheumatoid factor and its clinical significance].
We set up a dot immunobinding assay method for determination of IgM-, IgG- and IgA-rheumatoid factor (RF) and report here the results in 86 patients with rheumatoid arthritis (RA). It was found that the positive rates of IgM-, IgG- and IgA-RF were 59.7%, 54.7% and 17.4% respectively. The total positive rate was 72.8%. The positive rate using the Latex method was only 43.0%. Our results reveal that dot immunobinding assay for RF is more sensitive than the Latex method. Furthermore, there was a profound correlation of IgG-RF with active stage and the frequency of extra-artivular manifestations in RA patients.
Zhou
S
S
PUMC Hospital, CAMS and PUMC, Beijing.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood*; D002648:Child; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012217:Rheumatoid Factor / Q000097:blood* / Q000145:classification
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor