title: Fructose 1,6-bisphosphate prevents oxidative stress in the isolated and perfused rat heart.
abstract: Rat hearts were perfused with the Langendorff technique at constant flux in the presence of the oxidizing agents hydrogen peroxide and diamide. Fructose 1,6-bisphosphate strongly prevented the decline of heart contractility due to the infusion of these oxidizing agents. On the other hand, fructose 1,6-bisphosphate had no effect on the release of total glutathione into the perfusate but prevented the loss of lactate dehydrogenase indicating a protective effect on cell membranes. Comparing the cytosolic and mitochondrial loss of glutathione, fructose 1,6-bisphosphate exerted a beneficial action only on the mitochondrial fraction. Several mechanisms of action have been considered to explain the protective action of fructose 1,6-bisphosphate. In our experimental conditions fructose 1,6-bisphosphate might stimulate its own production giving rise to dihydroxyacetone phosphate, that, after reduction to glycerol 3-phosphate, can permeate the mitochondrial membrane with the final production of energy.
authors.affiliation: Dipartimento di Chimica Biologica, Università di Padova, Italy.
authors.forename: M P
authors.initials: MP
authors.lastname: Rigobello
authors.forename: L
authors.initials: L
authors.lastname: Galzigna
authors.forename: A
authors.initials: A
authors.lastname: Bindoli
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D003958:Diamide; D005635:Fructosediphosphates / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000889:Anti-Arrhythmia Agents; D005635:Fructosediphosphates; D003958:Diamide; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; C029063:fructose-1,6-diphosphate