title: Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein.
abstract: The human CCG1 gene, encoding CCG1/TAFII250/p250, was isolated by complementing tsBN462, a mutant BHK21 cell line that shows cell-cycle arrest at high temperature. Using the cDNA as a probe, the locations of exon-intron junctions were determined in the genomic DNA. Thirty-eight exons ranging from 68 to 219 bp in size were found. All the exon-intron junctions followed the GT-AG rule. Using a newly developed method, we performed a module analysis of the CCG1 protein. The functional domain previously predicted in CCG1 was further confirmed to be encoded in a single predicted module that is the minimal functional unit in the protein. The boundaries of the predicted modules show a close correlation to the intron/exon junction of CCG1. The entire gene, at least 110 kb long, has been recovered in a YAC, which provides a route to the further study of module function.
authors.affiliation: Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakashima
authors.forename: T
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: H
authors.initials: H
authors.lastname: Sunamoto
authors.forename: K
authors.initials: K
authors.lastname: Yura
authors.forename: S
authors.initials: S
authors.lastname: Tomoda
authors.forename: M
authors.initials: M
authors.lastname: Go
authors.forename: J
authors.initials: J
authors.lastname: Kere
authors.forename: D
authors.initials: D
authors.lastname: Schlessinger
authors.forename: T
authors.initials: T
authors.lastname: Nishimoto
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002453:Cell Cycle / Q000235:genetics*; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics
chemical_list: D035362:Transcription Factor TFIID; D014157:Transcription Factors