[Studies of purification, inactivity and immunogenicity of antigenically chimeric poliovirus].
Purified viruses were inactivated with 1:4000 formalin at 37 degrees C for 12d. Before and after inactivation, XF414 with anti-PV-1 and anti-PV-2, XF3 with anti-PV-1 and anti-PV-3 were observed by immunoelectron microscopy and the results were positive. Neutralization inhibition test and D-Ag unit detection were conducted, and the results were as follow: XF414 had type 1 D-Ag 15971 units/ml and type 2 D-Ag 15644 units/ml; XF3 had type 1 D-Ag 13314 units/ml and type 3 D-Ag 15971 units/ml. When rabbits were immunized with XF414 and XF3, they produced bivalent antiserum; i.e. anti-PV-1 and anti-PV-2 induced by XF414 and anti-PV-1 and anti-PV-3 induced by XF3.
Cai
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Institute of Medical Biology, CAMS, Kunming.
Dai
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C
D000818:Animals; D000956:Antigens, Viral; D002678:Chimera; D009500:Neutralization Tests; D017955:Poliovirus / Q000235:genetics* / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology; D011817:Rabbits; D014613:Vaccines, Attenuated
D000956:Antigens, Viral; D011054:Poliovirus Vaccine, Inactivated; D014613:Vaccines, Attenuated