title: Testis-specific transcription start site in the aspartate aminotransferase housekeeping gene promoter.
abstract: We have studied the expression and regulation of the rat testis cytosolic aspartate aminotransferase gene. The cytosolic aspartate aminotransferase activity was 5-fold lower in the testis than in the liver and kidney. A 1.9-kilobase mRNA form was detected in the rat testis in contrast to the 2.1- and 1.8-kilobase forms present in other organs. Using Northern blot and S1 mapping analyses, we found that the proximal polyadenylation site was almost exclusively used in the testis as opposed to other organs where the distal site was preferentially used. RNase protection and primer extension analysis showed that transcription was initiated at multiple sites in all organs, but the pattern of those start sites was different in the testis; in particular, a novel transcription start site was specifically detected in this organ (at position -115 from the translation start site). This site was first observed in 29-day-old rats and was maximally utilized in the adult testis. DNase I footprinting using testis nuclear extracts revealed the presence of three sites of DNA-protein interaction in the 250-base pair proximal promoter, a pattern similar to the one found using liver nuclear extracts. However, the proteins bound had different properties as shown by gel retardation experiments. We conclude that the pattern of transcription initiation and the polyadenylation site selection of a housekeeping gene can be tissue-specific.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U-99, Hôpital Henri Mondor, Creteil, France.
authors.forename: C
authors.initials: C
authors.lastname: Toussaint
authors.forename: B
authors.initials: B
authors.lastname: Bousquet-Lemercier
authors.forename: M
authors.initials: M
authors.lastname: Garlatti
authors.forename: J
authors.initials: J
authors.lastname: Hanoune
authors.forename: R
authors.initials: R
authors.lastname: Barouki
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003600:Cytosol / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013737:Testis / Q000201:enzymology*; D014158:Transcription, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D001219:Aspartate Aminotransferases