Serotyping Coxiella burnetii isolates from acute and chronic Q fever patients by using monoclonal antibodies.
Four mouse monoclonal antibodies reacting with Coxiella burnetti lipopolysaccharide antigens were produced and used in serotyping 17 C. burnetii isolates from acute Q fever and Q fever endocarditis patients in France. Two monoclonal antibodies (1B2 and 3B6) were considered specific for the Priscilla strain, a representative of Q fever endocarditis isolates, and did not react with the Nine Mile strain, which is representative of acute Q fever isolates. Monoclonal antibodies Nos. 1B2 and 3B6 reacted with 75% (3/4) acute Q fever isolates and 85% (11/13) of endocarditis isolates from France. It is reasonable to conclude that Priscilla-like strains cause both acute Q fever and Q fever endocarditis. The hypothesis that Priscilla-like strains only are associated with Q fever endocarditis should be reconsidered.
Yu
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Unité des Rickettsies, Faculté de Medécine, CNRS EP J 0054, Marseille, France.
Raoult
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D000208:Acute Disease; D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D002908:Chronic Disease; D016997:Coxiella burnetii / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D004697:Endocarditis, Bacterial / Q000382:microbiology; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D011778:Q Fever / Q000382:microbiology*; D012703:Serotyping
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides