title: Nitric oxide destroys zinc-sulfur clusters inducing zinc release from metallothionein and inhibition of the zinc finger-type yeast transcription activator LAC9.
abstract: Nitric oxide, generated from S-nitrosocysteine or applied as gas mediates metal ion release from the Zn2+/Cd(2+)-complexing protein metallothionein via oxidation of SH-groups. Time-dependent S-nitrosylation and subsequent disulfide formation of metallothionein are demonstrated. Furthermore, nitric oxide inhibits DNA binding activity of the yeast transcription factor LAC9 containing a zinc finger like DNA binding domain. These results show that nitric oxide interacts with and destroys zinc-sulfur clusters in proteins.
authors.affiliation: Medical Department, Heinrich-Heine University, Düsseldorf, Fed. Rep. Germany.
authors.forename: K D
authors.initials: KD
authors.lastname: Kröncke
authors.forename: K
authors.initials: K
authors.lastname: Fehsel
authors.forename: T
authors.initials: T
authors.lastname: Schmidt
authors.forename: F T
authors.initials: FT
authors.lastname: Zenke
authors.forename: I
authors.initials: I
authors.lastname: Dasting
authors.forename: J R
authors.initials: JR
authors.lastname: Wesener
authors.forename: H
authors.initials: H
authors.lastname: Bettermann
authors.forename: K D
authors.initials: KD
authors.lastname: Breunig
authors.forename: V
authors.initials: V
authors.lastname: Kolb-Bachofen
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors; D005656:Fungal Proteins / Q000037:antagonists & inhibitors*; D006736:Horses; D066298:In Vitro Techniques; D008668:Metallothionein / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000633:toxicity*; D010084:Oxidation-Reduction; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D014157:Transcription Factors / Q000037:antagonists & inhibitors; D015032:Zinc / Q000378:metabolism*; D016335:Zinc Fingers / Q000187:drug effects
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C058596:LAC9 protein, Kluyveromyces lactis; D013438:Sulfhydryl Compounds; D014157:Transcription Factors; D009569:Nitric Oxide; D013455:Sulfur; D008668:Metallothionein; D015032:Zinc