title: Rat Sertoli cell aromatase cytochrome P450: regulation by cell culture conditions and relationship to the state of cell differentiation.
abstract: Primary cultures of immature rat Sertoli cells in plastic dishes are highly responsive to follicle stimulating hormone (FSH) and its second messenger, cAMP, in metabolizing testosterone to estradiol, thus indicating the presence of an active, hormone-regulated aromatase cytochrome P450 (P450arom). However, in vivo studies indicated that P450arom is FSH-responsive only in very young animals, where the cells have not yet differentiated, but they lose this ability later on in development. Sertoli cells grown on Matrigel (a reconstituted basement membrane), laminin (a basement membrane component), or in bicameral chambers coated with Matrigel, assume structural and functional characteristics more similar to that of in vivo differentiated Sertoli cells. When the cells were cultured on laminin or Matrigel, the FSH- and cAMP-induced estradiol production was greatly reduced by 30 and 60%, respectively. When Sertoli cells were cultured in bicameral chambers coated with Matrigel, no induction of testosterone aromatization by FSH or cAMP was observed. However, FSH-induced cAMP formation was greater when the cells were cultured on basement membrane or in the chambers than on plastic dishes. These results suggest that culture conditions favoring the assumption by Sertoli cells of a phenotype closer that of the differentiated cells in vivo (tall columnar and highly polarized) suppress the induction of P450arom by FSH and cAMP. We then examined the mechanism(s) by which cell phenotype affects p450arom activity. Northern blot analyses of Sertoli cell RNA revealed one major band of 1.9 Kb and two minor bands of 3.3 and 5.2 Kb. However, there were no changes at the level of the expression of P450arom messenger RNA under the different culture conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cell Biology, Georgetown University Medical Center, Washington, D.C. 20007.
authors.forename: V
authors.initials: V
authors.lastname: Papadopoulos
authors.forename: M C
authors.initials: MC
authors.lastname: Jia
authors.forename: M
authors.initials: M
authors.lastname: Culty
authors.forename: P F
authors.initials: PF
authors.lastname: Hall
authors.forename: M
authors.initials: M
authors.lastname: Dym
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen; D004247:DNA / Q000032:analysis; D004338:Drug Combinations; D004958:Estradiol / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007797:Laminin; D008297:Male; D010969:Plastics; D011509:Proteoglycans; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D013739:Testosterone / Q000378:metabolism
chemical_list: D004338:Drug Combinations; D007797:Laminin; D010969:Plastics; D011509:Proteoglycans; D012333:RNA, Messenger; C059485:matrigel; D013739:Testosterone; D004958:Estradiol; D003994:Bucladesine; D005640:Follicle Stimulating Hormone; D003094:Collagen; D004247:DNA; D001141:Aromatase