Identification of the receptor for a novel M(r) 78,000 "invasion stimulating factor" from metastatic human prostatic PC-3 ML clones.
A M(r) 78,000 protein (reduced), termed invasion stimulating factor (ISF), was purified from the conditioned medium of a bone metastasizing human prostatic PC-3 ML clone (M. E. Stearns and M. Stearns. Cancer Metastasis Rev., 12: 39-52, 1993). Scatchard analysis and affinity cross-linking studies revealed that the producer PC-3 ML cells expressed a receptor binding site (M(r) approximately 115,000). We found a Kd approximately 425 pM and about 22,000 sites/cell. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiograms confirmed that the PC-3 ML cells expressed the receptor, whereas the ISF non-producing, noninvasive PC-3 clones (i.e., 3-4 x N.I. PC-3 cells) failed to express the ISF receptor. We conclude that a unique ISF autocrine loop characterizes the bone metastatic PC-3 ML cells.
Wang
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Department of Pathology, Medical College of Pennsylvania, Philadelphia 19129.
Stearns
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Stearns
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D001859:Bone Neoplasms / Q000556:secondary; D002465:Cell Movement; D017364:Collagenases / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9; D008970:Molecular Weight; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D018121:Receptors, Cytokine / Q000032:analysis*
D009363:Neoplasm Proteins; D018121:Receptors, Cytokine; D017364:Collagenases; D020780:Matrix Metalloproteinase 9; D005956:Glucose-6-Phosphate Isomerase