Ca2+ influx gated by inositol-3,4,5,6-tetrakisphosphate in NIH/3T3 fibroblasts.
The functional role of three inositol tetrakisphosphate isomers on Ca2+ influx was examined by intracellular application in NIH/3T3 mouse fibroblasts. Infusion of inositol-1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), inositol-1,3,4,6- tetrakisphosphate, and inositol-3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4) from the patch pipette into the cytoplasm produced a transient increase in cytoplasmic free Ca2+ concentration by fura-2 recording in extracellular Ca(2+)-dependent fashion. Stimulation of NIH/3T3 cells with bradykinin increased the levels of Ins(1,3,4,5)P4 and Ins(3,4,5,6)P4. These results suggest that mouse fibroblast cells possess a Ca2+ influx pathway gated with naturally occurring inositol tetrakisphosphates in response to stimulation with bradykinin.
Hashii
M
M
Department of Biophysics, Kanazawa University School of Medicine, Japan.
Hirata
M
M
Ozaki
S
S
Nozawa
Y
Y
Higashida
H
H
D016475:3T3 Cells; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D011905:Genes, ras; D007295:Inositol Phosphates / Q000502:physiology*; D051379:Mice; D015290:Second Messenger Systems
D007295:Inositol Phosphates; C081652:inositol-3,4,5,6-tetrakisphosphate; D001920:Bradykinin; D002118:Calcium