Detection of Borrelia burgdorferi antigens in cerebrospinal fluid.
We examined CSF for Borrelia burgdorferi antigens using antigen-capture ELISA and Western (immuno) blot. Antigen-capture ELISA was positive in 38 of 77 (49%) CSF samples obtained from neurologic patients with presumed B burgdorferi infection, compared with one of 34 (3%) CSF samples obtained from other neurologic disease controls who came from a region endemic for Lyme disease. Western immunoblot was positive for B burgdorferi antigens in 12 of 22 (55%) CSF samples from the B burgdorferi infected groups, compared with none of 11 CSF samples from the control group. CSF antigen detection should prove helpful in evaluating patients for suspected neurologic Lyme disease.
Coyle
P K
PK
Department of Neurology, SUNY at Stony Brook 11794.
Deng
Z
Z
Schutzer
S E
SE
Belman
A L
AL
Benach
J
J
Krupp
L B
LB
Luft
B
B
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000134:cerebrospinal fluid*; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial