Canine distemper virus replication in cells on microcarriers.
Chick embryo and Vero cells were grown on Gelaspher M gelatin microcarriers in suspension culture. The microcarriers had no adverse effects on cell morphology and growth. Microcarrier cell cultures were used for large-scale production of canine distemper virus. Virus yields (TCID50 per ml) were more than 10-times higher as compared to stationary cell culture.
Lesko
J
J
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Veber
P
P
Duseková
E
E
D000818:Animals; D001709:Biotechnology; D002478:Cells, Cultured; D002642:Chick Embryo; D004217:Distemper Virus, Canine / Q000254:growth & development / Q000502:physiology*; D005780:Gelatin; D010316:Particle Size; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D005780:Gelatin