title: Aspergillus fumigatus metalloproteinase that hydrolyses native collagen: purification by dye-binding chromatography.
abstract: A proteinase was purified from the human pathogenic fungus Aspergillus fumigatus. The four chromatographic steps, a "negative" dye column, a "positive" dye column, hydroxyapatite Ultrogel, and modified TSK gel (HW 55), gave a 14% overall yield. The protein migrated as a single band on SDS-PAGE and isoelectric focusing, with an M(r) of 82,000 and a pI of 5.6. Inhibitor studies suggested that the enzyme was a metalloproteinase. It hydrolyzed phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg and cleaved native rat type I collagen.
authors.affiliation: Institut Pasteur, Unité de Mycologie, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Ibrahim-Granet
authors.forename: O
authors.initials: O
authors.lastname: Bertrand
authors.forename: J P
authors.initials: JP
authors.lastname: Debeaupuis
authors.forename: T
authors.initials: T
authors.lastname: Planchenault
authors.forename: M
authors.initials: M
authors.lastname: Diaquin
authors.forename: B
authors.initials: B
authors.lastname: Dupont
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001232:Aspergillus fumigatus / Q000201:enzymology*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000302:isolation & purification* / Q000378:metabolism; D004396:Coloring Agents; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D007525:Isoelectric Focusing; D008666:Metalloendopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats
chemical_list: D004396:Coloring Agents; D005656:Fungal Proteins; D003094:Collagen; D017364:Collagenases; D008666:Metalloendopeptidases