title: The role of dynamic and morphological studies in the assessment of potential liver donors.
abstract: In a prospective study, 66 donor livers were evaluated by monoethylglycinexylidide (MEGX) dynamic clearance and semiquantitative scoring of pathological changes in liver biopsies. The median MEGX level in 63 donors was 89 mcg/L (range 16-250 mcg/L); fifteen had MEGX levels < 50 mcg/L, 17 between 50 and 90 mcg/L, and 31 > 90 mcg/L. There were no cases of primary nonfunction, and no deaths were related to poor graft function. There was no statistically significant difference in peak aspartate aminotransferase (AST), day 5 AST, peak bilirubin, or lowest prothrombin time among the 3 groups. Liver biopsies were assessed in 61 donors: 33 (54%) were normal and 17 (28%) showed mild, 8 (13%) showed moderate, and 3 (5%) showed severe steatosis. Postperfusion biopsy assessing the extent of preservation injury was essentially normal or showed minimal change in 16 (26%), mild change in 29 (48%), moderate in 13 (21%) and severe abnormalities in 3 (5%). The latter 3 biopsies all had severe steatosis. There was no significant difference in early graft function or outcome between moderate/severe groups and normal/minimal groups, although the former had a higher peak AST (P < 0.02) and peak bilirubin (P < 0.004). This detailed prospective analysis suggests that MEGX and the morphological studies may assist in the assessment of potential liver donors but they do not provide a basis on which grafts should be discarded.
authors.affiliation: Liver and Hepatobiliary Unit, Queen Elizabeth Hospital, Birmingham, UK.
authors.forename: K
authors.initials: K
authors.lastname: Karayalçin
authors.forename: D F
authors.initials: DF
authors.lastname: Mirza
authors.forename: R F
authors.initials: RF
authors.lastname: Harrison
authors.forename: R F
authors.initials: RF
authors.lastname: Da Silva
authors.forename: S G
authors.initials: SG
authors.lastname: Hubscher
authors.forename: A D
authors.initials: AD
authors.lastname: Mayer
authors.forename: J A
authors.initials: JA
authors.lastname: Buckels
authors.forename: P
authors.initials: P
authors.lastname: McMaster
mesh_terms: D000293:Adolescent; D000328:Adult; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis; D006801:Humans; D007223:Infant; D008012:Lidocaine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D016031:Liver Transplantation / Q000473:pathology* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D014019:Tissue Donors
chemical_list: D008012:Lidocaine; C001173:monoethylglycinexylidide; D001219:Aspartate Aminotransferases