Isolation and mapping of microsatellites from a library microdissected from the Werner syndrome region, 8p11.2-p22.
We have constructed a new genetic linkage map of the Werner syndrome (WRN) region, using microsatellites from a library which was developed by a chromosome microdissection and enzymatic amplification method. These microsatellites were used to genotype members of CEPH families using a simplified detection system of polymerase chain reaction (PCR) products. Two-point analysis was used to assign 4 microsatellite markers relative to each marker and other markers reported in the CEPH public data base. We confirmed that these 4 markers are located to the WRN region, 8p11.2-p22. Such microsatellites microdissected from the definite chromosome region may be useful for positional cloning.
Nagano
K
K
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Nakura
J
J
Kihara
K
K
Ye
L
L
Kamino
K
K
Mitsuda
N
N
Ohta
T
T
Jinno
Y
Y
Niikawa
N
N
Miki
T
T
D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D004276:DNA, Satellite / Q000235:genetics / Q000302:isolation & purification*; D008040:Genetic Linkage; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014898:Werner Syndrome / Q000235:genetics*
D004276:DNA, Satellite