title: Isolation and sequence of the t-RNA ligase-encoding gene of Candida albicans.
abstract: The gene encoding tRNA ligase from Candida albicans was isolated from a genomic library by complementation of a Saccharomyces cerevisiae strain containing a disrupted structural gene, RLG1, encoding tRNA ligase. The cloned gene also complements a temperature-sensitive allele of RLG1. Sequence analysis revealed a single 2499-nt coding region. The gene encodes a protein of 833 amino acids that is 42% identical to S. cerevisiae tRNA ligase. Hybridization to chromosomes of C. albicans separated by pulsed-field gel electrophoresis located the gene to chromosome 1, the smallest C. albicans chromosome.
authors.affiliation: Department of Microbial Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.
authors.forename: J
authors.initials: J
authors.lastname: Baymiller
authors.forename: S
authors.initials: S
authors.lastname: Jennings
authors.forename: B
authors.initials: B
authors.lastname: Kienzle
authors.forename: J A
authors.initials: JA
authors.lastname: Gorman
authors.forename: R
authors.initials: R
authors.lastname: Kelly
authors.forename: J E
authors.initials: JE
authors.lastname: McCullough
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics*; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000302:isolation & purification; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D011130:RNA Ligase (ATP) / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004271:DNA, Fungal; D011130:RNA Ligase (ATP)