Formation of 22 and 24 carbon 6-desaturated fatty acids from exogenous deuterated arachidonic acid is activated in THP-1 cells at high substrate concentrations.
Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[2H0] ratios higher than in AA. At high AA concentration preferential conversion to elongated fatty acids with 5 unsaturations, through a 6 desaturase takes place and the 4-desaturated 22:5 appears to be formed through beta-oxidation of 24:5.
Caruso
D
D
Institute of Pharmacological Sciences, University of Milano, Italy.
Risé
P
P
Galella
G
G
Regazzoni
C
C
Toia
A
A
Galli
G
G
Galli
C
C
D016718:Arachidonic Acid / Q000737:chemistry / Q000378:metabolism*; D002244:Carbon / Q000737:chemistry; D002460:Cell Line; D003903:Deuterium / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D013379:Substrate Specificity
D005227:Fatty Acids; D016718:Arachidonic Acid; D002244:Carbon; D003903:Deuterium