Progesterone receptor and hsp90 are not complexed in intact nuclei.
In hypotonic cell extract (cytosol), unliganded progesterone receptor (PR) is known to form an oligomeric complex with heat shock protein 90 (hsp90), and this complex does not bind to DNA. Since ligand binding has been shown to render the complex less stable in vitro, it has been proposed that ligand binding regulates DNA binding and receptor activity in vivo by altering the stability of the oligomeric complex. However, there is no direct evidence as to whether this oligomeric complex is present in vivo. The present study addressed this problem. First, we used an immunoelectron-microscopic technique and monoclonal antibodies to ascertain the location of PR and hsp90 in chick oviduct cells. Hsp90 was found in the cytoplasm and PR in the nucleus. To study the relative affinities of the PR and hsp90 antibodies, we then constructed a chimeric protein (PR-hsp90), which was expressed in the HeLa cells. Both hsp90 and PR antigens of the chimera were detected in the nuclei with the same intensity, which indicates that the antibodies have equal sensitivities in detecting their antigens. This suggests that if significant amounts of nuclear hsp90 were present in intact cells, it should have been detected by our method. Our results indicate that the PR does not exist in vivo as an oligomeric, nonDNA-binding form in the cell nuclei and that the oligomeric form found in tissue extracts is possibly formed during tissue processing.
Pekki
A
A
Department of Biomedical Science, University of Tampere, Finland.
Ylikomi
T
T
Syvälä
H
H
Tuohimaa
P
P
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002645:Chickens; D003593:Cytoplasm / Q000378:metabolism; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D016253:Microscopy, Immunoelectron; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism; D011980:Receptors, Progesterone / Q000276:immunology / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity
D000906:Antibodies; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D011980:Receptors, Progesterone; D011993:Recombinant Fusion Proteins