Alterations in NF kappa B/Rel family proteins in splenic T-cells from tumor-bearing mice and reversal following therapy.
It has recently been shown that T-cell signal transduction molecules are altered in tumor-bearing mice. We have examined the expression of NF kappa B/Rel family proteins in T-cells from mice bearing Renca, a murine renal carcinoma. T-cells from Renca-bearing mice expressed undetectable levels of nuclear c-Rel, NF kappa B p65, and p50; however, two shorter forms of p50 (p48 and p46), truncated at the NH2-terminus, were present exclusively in the nucleus and were able to bind DNA. These T-cells have reduced expression of gamma-interferon mRNA. In mice successfully treated with flavone 8-acetic acid and recombinant human interleukin 2, the T-cells expressed normal levels of all three nuclear NF kappa B/Rel proteins. These results suggest that alterations in transcription factors may accompany changes in signal transduction molecules in T-cells from tumor-bearing animals; however, the changes are reversed with successful biological therapy.
Ghosh
P
P
Laboratory of Experimental Immunology, NCI-FCRDC, Frederick, Maryland 21702-1201.
Sica
A
A
Young
H A
HA
Ye
J
J
Franco
J L
JL
Wiltrout
R H
RH
Longo
D L
DL
Rice
N R
NR
Komschlies
K L
KL
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001483:Base Sequence; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000628:therapy; D002467:Cell Nucleus / Q000737:chemistry; D004586:Electrophoresis; D005419:Flavonoids / Q000627:therapeutic use; D007371:Interferon-gamma / Q000032:analysis; D007376:Interleukin-2 / Q000627:therapeutic use; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000628:therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000737:chemistry*
D000970:Antineoplastic Agents; D005419:Flavonoids; D007376:Interleukin-2; D016328:NF-kappa B; D012333:RNA, Messenger; D007371:Interferon-gamma; C049328:flavone acetic acid