A simple method for the preparation of furosine and pyridosine reference material.
After heating N alpha-acetyllysine and glucose for 4 h at 90 degrees C in the dry state and subsequent acid hydrolysis with 7.8 N HCl, preparative fractionation of the dihydrochlorides of furosine and pyridosine was achieved by cation-exchange chromatography. The lysine derivatives could be prepared with high yield and sufficient purity for the use as reference material.
Henle
T
T
Lehrstuhl für Milchwissenschaft, Technische Universität München, Freising-Weihenstephan, Germany.
Walter
A W
AW
Klostermeyer
H
H
D000596:Amino Acids / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D005947:Glucose; D006031:Glycosylation; D006358:Hot Temperature; D006868:Hydrolysis; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D012015:Reference Standards
D000596:Amino Acids; C018948:furosine; C019068:pyridosine; C016949:N-epsilon-acetyllysine; D005947:Glucose; D008239:Lysine