Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant.
We have utilized a transendothelial lymphocyte chemotaxis assay to identify and purify a lymphocyte chemoattractant in supernatants of mitogen-stimulated peripheral blood mononuclear cells. Amino acid sequence analysis revealed identity with monocyte chemoattractant protein 1 (MCP-1), a chemoattractant previously thought to be specific for monocytes. Recombinant MCP-1 is chemoattractive for purified T lymphocytes and for CD3+ lymphocytes in peripheral blood lymphocyte preparations. The T-cell response to MCP-1 is dose-dependent and chemotactic, rather than chemokinetic. Phenotyping of chemoattracted T lymphocytes shows they are an activated memory subset. The response to MCP-1 by T lymphocytes can be duplicated in the absence of an endothelial monolayer and the majority of T-lymphocyte chemotactic activity in mitogen-stimulated peripheral blood mononuclear cell supernatants can be neutralized by antibody to MCP-1. Thus, MCP-1 is the major lymphocyte chemoattractant secreted by mitogen-stimulated peripheral blood mononuclear cells and is capable of acting as a potent T-lymphocyte, as well as monocyte, chemoattractant. This may help explain why monocytes and T lymphocytes of the memory subset are always found together at sites of antigen-induced inflammation.
Carr
M W
MW
Committee on Immunology, Harvard Medical School, Boston, MA.
Roth
S J
SJ
Luther
E
E
Rose
S S
SS
Springer
T A
TA
D000595:Amino Acid Sequence; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D016130:Immunophenotyping; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016176:T-Lymphocyte Subsets / Q000502:physiology*
D018932:Chemokine CCL2; D002630:Chemotactic Factors