Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H(+)-ATPase.
Bafilomycin A1, a potent selective inhibitor of vacuolar H(+)-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1 for 50% inhibition of cell growth ranged from 10 to 50 nM. The dose response was nearly parallel with that of the bafilomycin A1-induced lysosomal pH increase. The degree of pH increase for growth inhibition produced by bafilomycin A1 was similar to that produced by NH4Cl in which little difference was recognized in effect among cell types.
Ohkuma
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.
Shimizu
S
S
Noto
M
M
Sai
Y
Y
Kinoshita
K
K
Tamura
H
H
D016475:3T3 Cells; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016466:CHO Cells; D002455:Cell Division / Q000187:drug effects*; D002461:Cell Line, Transformed; D006224:Cricetinae; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism; D018942:Macrolides; D051379:Mice; D016716:PC12 Cells; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D014617:Vacuoles / Q000187:drug effects / Q000201:enzymology*
D000900:Anti-Bacterial Agents; D018942:Macrolides; D000643:Ammonium Chloride; C040929:bafilomycin A1; D006180:Proton-Translocating ATPases