Regulation of cucumber class III chitinase gene expression.
The chromosomal region encoding the acidic class III chitinase from cucumber has been isolated and characterized. As a result of an apparent gene triplication, the pathogen-induced gene (CHI2) is flanked by two closely related genes with complete open reading frames (ORF). The high level of conservation within the three ORFs suggests an essential role for each encoded protein in plant growth and development. The developmental and tissue-specific expression of RNA from each gene was analyzed using both gene-specific probes and RNA-PCR. The expression of each gene in response to various inducing treatments was also characterized. Only transcripts corresponding to CHI2 were detected. Chitinase mRNA abundance increased slightly following cycloheximide application; however, its potent induction by salicylic acid was inhibited by cycloheximide treatment.
Lawton
K A
KA
Department of Molecular Genetics, Ciba Agricultural Biotechnology, Research Triangle Park, NC 27709.
Beck
J
J
Potter
S
S
Ward
E
E
Ryals
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002688:Chitinases / Q000096:biosynthesis* / Q000235:genetics; D003513:Cycloheximide / Q000494:pharmacology; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D017343:Genes, Plant; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D016385:TATA Box; D014675:Vegetables / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D003513:Cycloheximide; D002688:Chitinases