[ELISA for detection of SC5b-9 levels in plasma and its clinical significance in SLE disease].
Using IgG from rabbit antiserum to human MAC neoantigens and SC5b-9, we established a sandwich ELISA for detecting SC5b-9 levels in human plasma. SC5b-9 was measured in 51 normal plasma samples and 40 plasma samples from patients with SLE. We found that SC5b-9 levels in 96.1% of the controls and in 78.9% of the patients with inactive SLE were below 450 ng/ml, while SC5b-9 levels in 81.0% of the patients with active SLE were above 450 ng/ml. This shows that SC5b-9 levels may be useful for diagnosing patients with clinically active SLE.
Kan
D
D
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
D000818:Animals; D015938:Complement Membrane Attack Complex; D003165:Complement System Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D011817:Rabbits
D015938:Complement Membrane Attack Complex; D006023:Glycoproteins; C047610:SC5b-9 protein complex; D003165:Complement System Proteins