title: Targeted development of microsatellite markers from inter-Alu amplification of YAC clones.
abstract: Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers from targeted regions of the genome. This can be achieved by selectively cloning and sequencing (CA)n-positive human inter-Alu sequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers.
authors.affiliation: Universidade Estadual de Campinas, (SP), Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: de Souza
authors.forename: V
authors.initials: V
authors.lastname: Allamand
authors.forename: I
authors.initials: I
authors.lastname: Richard
authors.forename: L
authors.initials: L
authors.lastname: Brenguier
authors.forename: I
authors.initials: I
authors.lastname: Chumakov
authors.forename: D
authors.initials: D
authors.lastname: Cohen
authors.forename: J S
authors.initials: JS
authors.lastname: Beckmann
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D005784:Gene Amplification; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004276:DNA, Satellite; D005819:Genetic Markers