Biochemical characterization of epidermal growth-factor receptor in Syrian hamster buccal pouch.
Binding of [125I]-epidermal growth factor (EGF) to the membrane of the adult hamster buccal pouch was specific and protein concentration dependent, and was significantly higher than that of rat buccal tissue (hamster versus rat; 12.1 +/- 0.9 versus 9.77 +/- 0.22 fmol/mg protein, mean +/- SD, n = 5, p < 0.01). The higher binding was due to a higher receptor number (Bmax). Western blot analysis using anti-EGF receptor antibody revealed four immunoreactive protein bands of 170, 150, 116 and 55 kDa in hamster buccal membrane preparations. In a separate experiment, the EGF concentration in the phosphate-buffered saline extract of the hamster submandibular gland was determined by radioimmunoassay; the level was significantly much lower (p < 0.01) than that of rat or mouse (hamster, rat, mouse; 24.2 +/- 2.6, 123 +/- 17, 5950 +/- 171 ng/g wet tissue; mean +/- SD, n = 3). The findings suggest that hamster buccal pouch is particularly well suited to the study of the biological functions of oral EGF receptor.
Wang
S L
SL
Dental Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Wu-Wang
C Y
CY
Correa
A
A
Brigham
M
M
Feng
J
J
Slomiany
A
A
Slomiany
B L
BL
D000818:Animals; D015153:Blotting, Western; D006224:Cricetinae; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000378:metabolism*; D011863:Radioimmunoassay
D008565:Membrane Proteins; D004815:Epidermal Growth Factor; D066246:ErbB Receptors