title: NMR structures of ferredoxin chloroplastic transit peptide from Chlamydomonas reinhardtii promoted by trifluoroethanol in aqueous solution.
abstract: The 32-amino acid transit peptide of the unicellular green alga Chlamydomonas reinhardtii ferredoxin has been synthesized and analysed by NMR spectroscopy and circular dichroism. The results show that while the peptide is unstructured in water, it undergoes an alpha-helix formation from residue 3 to 13 in a 30:70 molar-ratio mixture of 2,2,2-trifluoroethanol. The remainder of the peptide is still unstructured in CF3CD2OD/H2O mixtures, but is distributed on a side opposite to a hydrophobic ridge formed by Met5, Phe9 and Val13 on the induced alpha-helix. The NMR structures driven by 2,2,2-trifluoroethanol in aqueous solution, are discussed in terms of potent interactions with the chloroplast envelope and its translocation molecular machinery.
authors.affiliation: Institut de Biologie Structurale CEA-CNRS, Grenoble, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Lancelin
authors.forename: I
authors.initials: I
authors.lastname: Bally
authors.forename: G J
authors.initials: GJ
authors.lastname: Arlaud
authors.forename: M
authors.initials: M
authors.lastname: Blackledge
authors.forename: P
authors.initials: P
authors.lastname: Gans
authors.forename: M
authors.initials: M
authors.lastname: Stein
authors.forename: J P
authors.initials: JP
authors.lastname: Jacquot
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000737:chemistry* / Q000187:drug effects; D002736:Chloroplasts / Q000737:chemistry*; D002942:Circular Dichroism; D005288:Ferredoxins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011487:Protein Conformation; D021382:Protein Sorting Signals / Q000737:chemistry*; D012996:Solutions; D014270:Trifluoroethanol / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D005288:Ferredoxins; D021382:Protein Sorting Signals; D012996:Solutions; D014867:Water; D014270:Trifluoroethanol