Comparison of direct swim-up, mini-Percoll, and Sephadex G10 separation procedures.
A comparison was made of three sperm separation techniques--direct swim-up, mini-Percoll, and Sephadex G-10--on semen parameters, including count, % motility, forward progression, and hypoosmotic swelling (HOS) test scores. Overall, the best quality sperm (less debris, best % and quality motility, and best HOS scores) were noted with the direct swim-up procedure. Mini-Percoll resulted in the highest count, but the worst HOS score. Sephadex G-10 resulted in quality of semen specimen almost as good as the direct swim-up procedure, but did allow some noncellular debris and bacteria to filter through the column.
Bollendorf
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University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden.
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Katsoff
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Lurie
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D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D006801:Humans; D008297:Male; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology