Idiotype-specific autologous T-cell interactions restricted by HLA-DR.
T lymphocytes, primed in vitro to alloantigens were shown to acquire a profound capacity to stimulate autologous T-cell proliferative responses. Both PBLs and purified peripheral T cells responded to alloreactive aTLCs, suggesting that the T/T interaction did not require processing and presentation of antigen by APCs. The T/T interactions were intrinsically MHC restricted, since the autologous T-cell response was blocked by the addition of mAbs to HLA-DR. In secondary responses, primed T-cell lines responded with a higher magnitude to the priming aTLC relative to other aTLCs with different alloantigenic specificities. This specificity of response supports a model of idiotypic TcR recognition by the responding cells. Indeed, TcR protein purified from the cell surface of the priming aTLC could stimulate the primed T-cell lines in secondary responses. Reciprocal interactions between TcRs were ruled out. These data suggest that T-cell-mediated, MHC-restricted, TcR-specific, autologous T-cell responses may be important in peripheral immune regulation.
Garlie
N K
NK
Blood Research Institute, Immunogenetics Research Section, Milwaukee, Wisconsin.
Eckels
D D
DD
D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D006684:HLA-DR Antigens; D007130:Immunoglobulin Idiotypes; D007519:Isoantigens; D011948:Receptors, Antigen, T-Cell