Phosphorylation of farnesol by a cell-free system from Botryococcus braunii.
Farnesol was incorporated into squalene as well as botryococcenes when the alcohol was fed to the culture of Botryococcus braunii B race strain. In in vitro experiments with a 10,000 x g supernatant of cell homogenate, squalene was synthesized from farnesyl diphosphate in the presence of NADPH or NADH, but botryococcenes were not synthesized under the same conditions. A 100,000 x g pelet fraction was able to phosphorylate farnesol to give its mono- and diphosphate esters in a CTP dependent manner.
Inoue
H
H
Institute for Chemical Reaction Science, Tohoku University, Sendai, Japan.
Korenaga
T
T
Sagami
H
H
Koyama
T
T
Ogura
K
K
D003570:Cytidine Triphosphate / Q000378:metabolism; D056890:Eukaryota / Q000378:metabolism*; D005204:Farnesol / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010766:Phosphorylation; D013185:Squalene / Q000378:metabolism; D014315:Triterpenes / Q000378:metabolism
D014315:Triterpenes; D009243:NAD; D005204:Farnesol; D009249:NADP; D003570:Cytidine Triphosphate; D013185:Squalene