Retinoblastoma antioncogene is involved in the inhibition of myogenesis by polyomavirus large T antigen.
The expression of polyomavirus large T antigen in stably transfected C2 myoblast cells inhibits terminal differentiation without inducing a transformed phenotype. In the present work, we report on the lifting of this inhibition by a mutation that prevents polyomavirus large T antigen from binding to the product of the retinoblastoma susceptibility gene (p105 RB). In contrast with cells containing wild-type large T, those with the Rb binding site mutant large T showed the same up-regulation of myosine heavy chain and myogenin mRNA expression as control cells. Furthermore, we correlate the cell cycle alteration induced by polyomavirus large T antigen expression with the inability of the cells to undergo terminal differentiation.
Maione
R
R
Dipartimento di Biopatologia Umana, Università di Roma La Sapienza, Italy.
Fimia
G M
GM
Holman
P
P
Schaffhausen
B
B
Amati
P
P
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis / Q000378:metabolism*; D001483:Base Sequence; D002453:Cell Cycle; D002460:Cell Line; D016161:Genes, Retinoblastoma; D005822:Genetic Vectors; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D016160:Retinoblastoma Protein / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D016160:Retinoblastoma Protein