Investigation of the activation state of the X chromosome in non-Hodgkin's lymphomas.
Cytogenetic analysis of non-Hodgkin's lymphomas (NHLs) has previously revealed a high incidence of numerical abnormalities involving the X chromosome. We have now used a combination of fluorescence in situ hybridization (FISH) and Southern blot analysis of methylation to examine the activation state of additional X chromosomes in NHL. Although FISH analysis of X chromosome centromeres in interphase nuclei was complicated by a number of factors, such as cell-cycle position, there was evidence that more than one X chromosome was present in the active state in 4/9 NHL. Methylation studies were carried out using the M27 beta probe, which also suggested that more than one activated X chromosome was present in at least 2/7 NHL cases. The two approaches therefore provided evidence that in some cases of NHL, unlike sex-chromosome-syndrome individuals, additional X chromosomes may be present in the active state. These data support the suggestion that NHL-associated oncogenes might be located on the X chromosome.
Grierson
A J
AJ
Department of Clinical Oncology, University Medical School, Sheffield, UK.
Hammond
D W
DW
Goepel
J R
JR
Hancock
B W
BW
Goyns
M H
MH
D015139:Blotting, Southern; D002874:Chromosome Mapping; D015870:Gene Expression; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D008745:Methylation; D012729:Sex Chromosome Aberrations / Q000209:etiology; D014960:X Chromosome