Tyrosinase-based ruthenium dispersed carbon paste biosensor for phenols.
Highly sensitive biosensors for phenolic compounds, based on the incorporation of the enzyme tyrosinase within metal-dispersed carbon paste matrices are described. In particular, ruthenium-doped carbon pastes display excellent electrocatalytic activity for the reduction of the enzymatically generated quinone species. Hence, convenient quantitation of micromolar concentrations is feasible at 0.0 V (vs. Ag/AgCl reference), with a greatly enhanced response compared to conventional carbon-paste tyrosinase biosensors. The influence of various experimental variables is explored. Fast, sensitive and reproducible flow injection detection of phenolic compounds is illustrated. Analogous improvements are reported for tyrosinase-rich plant tissue electrodes.
Wang
J
J
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces 88003.
Lu
F
F
Lopez
D
D
D015374:Biosensing Techniques; D002244:Carbon; D002384:Catalysis; D014442:Monophenol Monooxygenase; D009824:Ointments; D010636:Phenols / Q000032:analysis*; D012428:Ruthenium
D009824:Ointments; D010636:Phenols; D002244:Carbon; D012428:Ruthenium; D014442:Monophenol Monooxygenase