Cholesterol measurement by reflotron dry chemistry in infants, children and adults.
Cholesterol values measured with the Reflotron dry chemistry (Boehringer Mannheim, Mannheim, Germany) and enzymatic (CHOD-PAP) routine method in 4150 venous blood samples from 580 infants aged 7 months, 1778 children aged 13 months to 19 years, and 1792 adults aged 18-63 years showed good correlation between the two assay systems (r > 0.92). However, the mean cholesterol concentrations were 4.5%, 3.7% and 2.3% lower in infants, children and adults, respectively, if measured with the Reflotron analyser as compared with values obtained with the CHOD-PAP method. These differences in the values were greater when cholesterol values were low (significance for the slope of regression line, p < 0.001). Values were below the detection limit of the Reflotron assay (2.59 mmol l-1) in 104 (4.4%) and 4 (0.2%) of all paediatric and adult samples, respectively. When measured with the CHOD-PAP method, 75 (72%) and 3 (75%) of these same samples showed values which exceeded the 2.59 mmol l-1 detection limit. We conclude that Reflotron dry chemistry analyser is precise in fast measurement of serum cholesterol both in all paediatric subjects and adults. A particular problem in paediatric age groups is that about 4% of children have values below the detection limit of the Reflotron analyser.
Lapinleimu
H
H
Cardiorespiratory Research Unit, University of Turku, Finland.
Viikari
J
J
Jokinen
E
E
Routi
T
T
Salo
P
P
Hollo
O
O
Niinikoski
H
H
Rönnemaa
T
T
Leino
A
A
Välimäki
I
I
D000293:Adolescent; D000328:Adult; D000375:Aging / Q000097:blood; D000632:Aminopyrine; D001800:Blood Specimen Collection / Q000379:methods; D002624:Chemistry, Clinical / Q000379:methods* / Q000706:statistics & numerical data; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000097:blood*; D002789:Cholesterol Oxidase; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D012016:Reference Values
D000632:Aminopyrine; D002784:Cholesterol; D002789:Cholesterol Oxidase