Multiple subcellular localization of bcl-2: detection in nuclear outer membrane, endoplasmic reticulum membrane, and mitochondrial membranes.
The use of biochemical fractionation, immunofluorescence laser-scanning confocal microscopy, and immunoelectron microscopy with mouse anti-human bcl-2 monoclonal antibody to analyze the subcellular localization of the bcl-2 gene product revealed the protein prominently in the nuclear envelope, endoplasmic reticulum membrane, and mitochondrial membranes. Electron microscopy at high magnification more precisely localized bcl-2 to the nuclear outer membrane as confirmed by the biochemical fractionation, as well as to mitochondrial outer and, to a lesser degree, inner membrane. This multisite membrane distribution of bcl-2 suggests an important role for this protein in several different membrane compartments.
Akao
Y
Y
Department of Anatomy and Biology, Osaka Medical College, Japan.
Otsuki
Y
Y
Kataoka
S
S
Ito
Y
Y
Tsujimoto
Y
Y
D001402:B-Lymphocytes / Q000737:chemistry*; D002461:Cell Line, Transformed; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D006801:Humans; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000737:chemistry*; D009685:Nuclear Envelope / Q000737:chemistry*; D011518:Proto-Oncogene Proteins / Q000032:analysis*
D011518:Proto-Oncogene Proteins