Glycosylphosphatidylinositol-anchored CD4 supports human immunodeficiency virus type 1 replication, but not cytopathic effect, in T-cell transfectants.
Despite equivalent p24 antigen production, HSB-2 T cells expressing glycosylphosphatidylinositol (GPi)-linked CD4 were productively infected without cell death or syncytium formation, unlike HSB-2 transfectants expressing wild-type CD4 (wtCD4). HSB-2 transfectants dually expressing wtCD4 and GPi-linked CD4 formed syncytia and died. Thus, wtCD4 expression is critical for human immunodeficiency virus cytopathic effect in HSB-2 transfectants.
Marshall
W L
WL
Division of Infectious Disease, Dana-Farber Cancer Institute, Boston, MA 02115.
Mittler
E S
ES
Avery
P
P
Lawrence
J P
JP
Finberg
R W
RW
D015704:CD4 Antigens / Q000378:metabolism*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D014162:Transfection; D014779:Virus Replication
D015704:CD4 Antigens; D017261:Glycosylphosphatidylinositols