title: MIP-1 alpha is a regulator of mitotic and meiotic DNA synthesis during spermatogenesis.
abstract: To find out whether macrophage inflammatory protein-1 alpha (MIP-1 alpha) has a role in the regulation of germ cell development, we studied its effects on spermatogenic stage-specific DNA synthesis in vitro. MIP-1 alpha increased the DNA synthesis of primitive type A2-4 spermatogonia and of premeiotic cells, whereas the DNA synthesis of more differentiated intermediate and type B spermatogonia was inhibited when cultured in the presence of MIP-1 alpha. An antibody against MIP-1 alpha cross-reacted with a protein of 15 kDa from every spermatogenic stage of rat seminiferous epithelium. Immunohistochemical studies with the same antibody revealed a complex pattern of MIP-1 alpha localization both in primitive and advanced spermatogenic cells. These observations suggest that MIP-1 alpha is a local regulator of mitotic and meiotic DNA synthesis.
authors.affiliation: Department of Anatomy, University of Turku, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Hakovirta
authors.forename: M
authors.initials: M
authors.lastname: Vierula
authors.forename: S D
authors.initials: SD
authors.lastname: Wolpe
authors.forename: M
authors.initials: M
authors.lastname: Parvinen
mesh_terms: D000818:Animals; D054407:Chemokine CCL4; D016207:Cytokines / Q000494:pharmacology / Q000502:physiology*; D004261:DNA Replication / Q000187:drug effects; D007985:Leydig Cells / Q000737:chemistry; D019402:Macrophage Inflammatory Proteins; D008297:Male; D008540:Meiosis / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D015846:Monokines / Q000494:pharmacology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012671:Seminiferous Tubules / Q000166:cytology / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013093:Spermatogonia / Q000187:drug effects
chemical_list: D054407:Chemokine CCL4; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D015846:Monokines