Effect of 13-hydroperoxyoctadecadienoic acid on prostaglandin synthesis in rabbit kidney medulla microsomes.
The effect of 13-hydroperoxyoctadecadienoic acid (13-HPODE) on the synthesis of prostaglandins (PGs) was examined in rabbit kidney medulla microsomes. Medulla microsomes were incubated with arachidonic acid in 0.1 M-Tris/HCl buffer (pH 8.0) containing reduced glutathione and hydroquinone and the PGE2, PGF2a and PGD2 formed were measured by high-pressure liquid chromatography using 9-anthryldiazomethane for derivatization. Under our incubation conditions rabbit kidney medulla was found to produce mainly PGE2. The addition of 13-HPODE inhibited the production of all three PGs to a similar extent. 13-Hydroxyoctadecadienoic acid did not suppress the formation of PGs, indicating the requirement of the hydroperoxy moiety for the inhibitory effect of 13-HPODE on PG formation. Experiments utilizing the native fatty acid linoleic acid and tert-butyl hydroperoxide suggested the importance of the fatty acid-derived hydroperoxide in PG synthesis by kidney medulla. We conclude that 13-HPODE is an inhibitor of renomedullary cyclooxygenase and may have functional effects within the kidney.
Fujita
T
T
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Nakajima
T
T
Matsuma
T
T
Nishida
H
H
Sakuma
S
S
Fujimoto
Y
Y
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D015237:Dinoprost / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis*; D007679:Kidney Medulla / Q000187:drug effects* / Q000378:metabolism*; D008041:Linoleic Acids / Q000494:pharmacology*; D008054:Lipid Peroxides; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D015230:Prostaglandin D2 / Q000096:biosynthesis*; D011817:Rabbits
D008041:Linoleic Acids; D008054:Lipid Peroxides; C038649:13-hydroperoxy-9,11-octadecadienoic acid; D016718:Arachidonic Acid; D015237:Dinoprost; D015232:Dinoprostone; D015230:Prostaglandin D2