title: Mitogenic stimulation of murine B lymphocytes by the N-acetyl-D-galactosamine specific bacterial lectin-like substance from Eikenella corrodens.
abstract: A N-acetyl-D-galactosamine (GalNAc) specific bacterial lectin-like substance from Eikenella corrodens 1073 (EcLS) was found to have potent mitogenic activity when cultured with splenocytes from BALB/c mice. The results indicated that B lymphocytes are the major cell type responding to EcLS. The mitogenic activity of EcLS was dose-dependent, and the optimal concentration was around 5 micrograms/ml. The mitogenic activity did not appear to be due to a bacterial endotoxin, as GalNAc inhibited the mitogenic activity of EcLS, but did not inhibit the activity of lipopolysaccharide isolated from E. corrodens. EcLS stimulated murine B lymphocytes not only to proliferate, but also to differentiate into antibody-secreting cells, as demonstrated by the production of immunoglobulin by B lymphocytes stimulated with EcLS. These findings suggest that EcLS is a novel lectin that not only induces B lymphocyte proliferation, but also differentiation.
authors.affiliation: Department of Conservative Dentistry, Tokushima University, School of Dentistry, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakae
authors.forename: H
authors.initials: H
authors.lastname: Yumoto
authors.forename: T
authors.initials: T
authors.lastname: Matsuo
authors.forename: S
authors.initials: S
authors.lastname: Ebisu
mesh_terms: D000116:Acetylgalactosamine / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001422:Bacterial Adhesion; D002454:Cell Differentiation; D002455:Cell Division; D016124:Eikenella corrodens / Q000276:immunology* / Q000472:pathogenicity; D016905:Gram-Negative Bacterial Infections / Q000209:etiology; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010510:Periodontal Diseases / Q000209:etiology
chemical_list: D037102:Lectins; D000116:Acetylgalactosamine