Electron microscopic localization of silver binding proteins in the cytoplasmic granules of guinea pig eosinophil.
A new electron optic configuration is described in the specific granules of eosinophil leukocytes. Application of the ammonium silver nitrate impregnation after treatment of intact cells with formaldehyde-OsO4 solution reveals regular deposits of 50 A-electron-opaque particles which are localized in the axial region of the central core. They contain acid-extractable proteins that seem to include the eosinophil basic proteins which participate to the local immune response of the host.
Gancevici
G
G
Cantacuzino Institute, Bucharest, Romania.
Petrovici
A
A
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure*; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000378:metabolism / Q000648:ultrastructure*; D006168:Guinea Pigs; D008854:Microscopy, Electron; D011485:Protein Binding; D012260:Ribonucleases; D016622:Silver Staining
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D012260:Ribonucleases