Immunodetection of latex antigens.
BACKGROUND
Protein antigens in latex products can cause type I reactions. In the past antigens have been measured by protein assays, high-performance liquid chromatography, RAST inhibition, and skin tests. We examined the use of a mouse monoclonal antibody (CRI-C) to latex in the detection of latex antigens.


METHODS
CRI-C was raised by standard techniques after immunization of a BALB/c mouse with ammoniated latex. Medical gloves were extracted and assayed with: (1) standard protein assays, (2) RAST inhibition assays with sera from health care workers allergic to latex and patients with spina bifida, (3) an ELISA with a biotinylated form of CRI-C (BiC). Reference proteins included bovine serum albumin for the protein assays and nonammoniated latex and affinity-purified C antigen for the immunoassays.


RESULTS
Among the protein assays, the best correlation was between the Bradford and bicinchoninic acid assays. In absolute numbers the Bradford assay produced the lowest results, and OD280 the highest. The OD280, BCA, and Bradford methods "detected" protein in vinyl gloves. The results of RAST inhibition and BiC ELISA correlated with the protein assays. These immunoassays appeared to be more specific than the protein assays.


CONCLUSIONS
The BiC ELISA is an easy and reproducible in vitro assay of relevant latex antigen. Clinical correlation will be required for validation.
Slater
J E
JE
Department of Allergy and Immunology, Children's National Medical Center, Washington, D.C. 20010.
Trybul
D E
DE
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis* / Q000302:isolation & purification; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D017575:Gloves, Protective; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007840:Latex; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification; D011852:Radioallergosorbent Test / Q000379:methods
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D007840:Latex; D010940:Plant Proteins; D001710:Biotin