Effects of an activator and an inhibitor of protein kinase C on serotonin receptors induced by rat brain mRNA in Xenopus oocytes.
The effects of bath-applied phorbol esters, protein kinase C (PKC) activators, and sphingosine, a PKC inhibitor, on membrane currents evoked by serotonin were studied in Xenopus oocytes injected with rat brain mRNA. The oscillatory Cl- current in response to bath-applied 50 nM serotonin was significantly depressed by 200 nM phorbol dibutyrate and remarkably augmented by 100 microM sphingosine. Both drugs have little effect either on the nonspecific Cl- current response induced by aluminum fluoride or on the cationic current response induced by 1 mM kainate.
Matsumoto
M
M
Molecular Neurobiology Unit, MRC Centre, Cambridge, UK.
Barnard
E A
EA
D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002712:Chlorides / Q000502:physiology; D004553:Electric Conductivity; D004789:Enzyme Activation; D005459:Fluorides / Q000494:pharmacology; D007608:Kainic Acid / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D012333:RNA, Messenger / Q000494:pharmacology*; D051381:Rats / Q000235:genetics; D011985:Receptors, Serotonin / Q000235:genetics*; D012701:Serotonin / Q000494:pharmacology; D013110:Sphingosine / Q000494:pharmacology; D014982:Xenopus laevis
D017607:Aluminum Compounds; D002712:Chlorides; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D012701:Serotonin; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D013110:Sphingosine; D005459:Fluorides; D007608:Kainic Acid; C032311:aluminum fluoride