Lysophosphatidylcholine, a component of stromal phospholipids, as a candidate vasoconstrictive factor in stroma-free hemoglobin.
Stroma-free hemoglobin (SFH) contains a trace amount of phospholipids, which has been implicated in the toxic reactions associated with SFH. We analyzed stromal phospholipids by high-performance liquid chromatography and found that SFH contained small quantities of lysophosphatidylcholine (LPC), which is known to be capable of producing a defect in endothelium-dependent arterial relaxation, in addition to major classes of constituent phospholipids in red cell membrane. LPC content was determined to be 1.65 nmol/ml (hemoglobin 8.1 g/dl). To evaluate the role of these stromal phospholipids in SFH-induced vasoconstriction, we next examined the effect of lipids on vascular tone in rabbit aortic strips. Preincubation with the crude lipid extract or the LPC purified from SFH significantly inhibited acetylcholine-induced relaxation in phenylephrine-precontracted tissues. The LPC-induced inhibition was reversed by incubation of the tissues in the absence of lipids, indicating the functional integrity of endothelium. From these results, we propose a possibility that LPC, a component of stromal phospholipids, is a candidate for vasoconstrictive factors present in SFH.
Nakai
K
K
Hokkaido Red Cross Blood Center, Sapporo, Japan.
Matsuda
N
N
Ohta
T
T
Amano
M
M
Takahashi
T A
TA
Sakuma
I
I
Kitabatake
A
A
Ito
S
S
Nakazato
Y
Y
Sekiguchi
S
S
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000032:analysis / Q000494:pharmacology*; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D010743:Phospholipids; D011817:Rabbits; D014661:Vasoconstriction / Q000187:drug effects
D006454:Hemoglobins; D008244:Lysophosphatidylcholines; D010743:Phospholipids; D010656:Phenylephrine; D000109:Acetylcholine