A simple method of chromium coating using a ferrotype plate for scanning electron microscopy.
A simple method of chromium coating was developed. A chromium ferrotype plate for drying photoprints was used as a target of a planar magnetron ion-sputter coater. As a result, a high quality chromium film was obtained on dried specimens at room temperature without cooling. The grain size, measuring 1-2 nm in diameter, was small enough for observations of ultrastructures such as F1 particles on the inner mitochondrial membrane. In routine studies under x 100,000, however, chromium coating was not recommended because of its low emission of secondary electrons.
Mitsushima
A
A
Department of Anatomy, Faculty of Medicine, Tottori University, Japan.
Inoué
T
T
D002857:Chromium; D007425:Intracellular Membranes / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008928:Mitochondria / Q000648:ultrastructure; D016626:Shadowing Technique, Histology / Q000379:methods*
D002857:Chromium