Genes encoding general initiation factors for RNA polymerase II transcription are dispersed in the human genome.
General transcription factors are required for accurate initiation of transcription by RNA polymerase II. Human cDNAs encoding subunits of these factors have been cloned and sequenced. Using fluorescence in situ hybridization (FISH), we show here that the genes encoding the TATA-box binding protein (TBP), TFIIB, TFIIE alpha, TFIIE beta, RAP30, RAP74 and the 62 kDa subunit, of TFIIH are located at the human chromosomal bands 6q26-27, 1p21-22, 3q21-24, 8p12, 13q14, 19p13.3 and 11p14-15.1, respectively. This dispersed localization of a group of functionally related gene provides insights into the molecular mechanism of human genome evolution and their possible involvement in human diseases.
Heng
H H
HH
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.
Xiao
H
H
Shi
X M
XM
Greenblatt
J
J
Tsui
L C
LC
D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D008214:Lymphocytes / Q000378:metabolism; D046911:Macromolecular Substances; D012319:RNA Polymerase II / Q000378:metabolism*; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000235:genetics*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
D015342:DNA Probes; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C066644:transcription factor TFIIE; D051758:Transcription Factor TFIIH; D012319:RNA Polymerase II; C056604:transcription factor TFIIF