Testing drugs on human osteoarthritic articular cartilage.
New drugs are generally developed against animal models of the human disease. Before they are subjected to clinical trials it might be helpful to be able to test whether they are as effective against the disease in human tissue as they were in animals. It is proposed that this can be achieved by the use of organ maintenance culture of the human diseased tissue, the relevant biochemical parameters being measured by quantitative cytochemistry. In the present studies differences between the effect of indomethacin and of the 'chondroprotective' drug diclofenac sodium, on human osteoarthritic cartilage, have been measured.
Chayen
J
J
Unit of Cellular Pharmacology and Toxicology, Robens Institute of Health and Safety, University of Surrey, Guildford, U.K.
Bitensky
L
L
Mehdizadeh
S
S
Dunham
J
J
Older
J
J
D000368:Aged; D000369:Aged, 80 and over; D000423:Alcian Blue; D001718:Birefringence; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D004008:Diclofenac / Q000627:therapeutic use*; D005260:Female; D006621:Hip Joint / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D015207:Osteoarthritis, Hip / Q000188:drug therapy* / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis / Q000378:metabolism; D015203:Reproducibility of Results; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000378:metabolism
D011509:Proteoglycans; D004008:Diclofenac; D014533:Uridine Diphosphate Glucose Dehydrogenase; D000423:Alcian Blue; D007213:Indomethacin