Application of the ligase chain reaction to the detection of nisinA and nisinZ genes in Lactococcus lactis ssp. lactis.
This paper reports on the application of the ligase chain reaction (LCR) to the specific detection of variants of the nisin structural gene (nisinA and nisinZ) in nisin producing strains of Lactococcus lactis ssp lactis. The LCR assay was used to screen nisin producing strains to determine which form of the nisin structural gene they contained. This method of differentiating the nisin structural gene variants provides a useful alternative to the only other available genetic differentiation, that of sequencing the gene.
Ward
L J
LJ
New Zealand Dairy Research Institute, Palmerston North.
Brown
J C
JC
Davey
G P
GP
D001483:Base Sequence; D011088:DNA Ligases; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005821:Genetic Techniques; D014644:Genetic Variation; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009561:Nisin / Q000096:biosynthesis / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial; D009561:Nisin; D011088:DNA Ligases