title: A method to detect enteroviruses in sewage sludge-amended soil using the PCR.
abstract: PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.
authors.affiliation: Department of Soil and Water Science, University of Arizona, Tucson 85721.
authors.forename: T M
authors.initials: TM
authors.lastname: Straub
authors.forename: I L
authors.initials: IL
authors.lastname: Pepper
authors.forename: M
authors.initials: M
authors.lastname: Abbaszadegan
authors.forename: C P
authors.initials: CP
authors.lastname: Gerba
mesh_terms: D004770:Enterovirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012722:Sewage; D012988:Soil Microbiology
chemical_list: D012722:Sewage