Selective advantage of normal erythrocyte production after bone marrow transplantation of alpha-thalassemic mice.
Anemia resulting from alpha-thalassemia in mice was corrected by transplantation of normal bone marrow cells following sublethal total body irradiation, resulting in partial hematopoietic chimerism with a preponderance of normal peripheral blood red cells. Peripheral blood red cell chimerism in recipients of graded numbers of bone marrow cells from sex-mismatched donors, determined by cytometric analysis, was directly compared with immature hematopoietic cell (CFU-S) chimerism and peripheral blood white cell chimerism. The latter two were assessed by fluorescent in situ hybridization with a murine Y-chromosome-specific probe. Peripheral blood white cell chimerism consistently corresponded with immature hematopoietic cell chimerism, emphasizing the selective advantage of normal red cell production in partially chimeric alpha-thalassemic mice.
van den Bos
C
C
Institute of Hematology, Erasmus University, Rotterdam, The Netherlands.
Kieboom
D
D
van der Sluijs
J P
JP
Baert
M R
MR
Ploemacher
R E
RE
Wagemaker
G
G
D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D016026:Bone Marrow Transplantation; D002678:Chimera; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology; D004920:Erythropoiesis / Q000502:physiology; D005260:Female; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014916:Whole-Body Irradiation; D017085:alpha-Thalassemia / Q000473:pathology / Q000628:therapy*