title: Collagen-binding recombinant fibronectin fragments containing type II domains.
abstract: Each of the two type II domains and four larger fragments, containing one or two type II domains of fibronectin, have been expressed in Escherichia coli. A special vector, containing a fragment encoding the cleavage site for Factor Xa, Ile-Glu-Gly-Arg, inserted immediately before the protein fragment of interest, was used. After treatment of the purified fusion proteins with reduced/oxidized glutathione, the correctly folded fibronectin fragments were released by proteolytic digestion with Factor Xa. The largest fragment, consisting of two type II and two type I domains, was the only fragment able to bind to immobilized gelatin.
authors.affiliation: Department of Chemistry, University of Aarhus, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Skorstengaard
authors.forename: T L
authors.initials: TL
authors.lastname: Holtet
authors.forename: M
authors.initials: M
authors.lastname: Etzerodt
authors.forename: H C
authors.initials: HC
authors.lastname: Thøgersen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003094:Collagen / Q000378:metabolism*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D004274:DNA, Recombinant; D005353:Fibronectins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D003094:Collagen