Study of the resistance of tumor-cell spheroids to penetration and lysis by activated effector cells.
Tumor spheroids and growing cell monolayers were used as 2- and 3-dimensional targets in the in vitro study of the interactions of colorectal tumor cells with various in vitro Il-2-activated lymphocyte effectors. The study examined tumor-cell susceptibility to the cytotoxic activity of effector cells in correlation with the ability of the effectors to infiltrate spheroids. No restriction of lymphocyte activity was found when the susceptibility of tumor cells to the panel of autologous and allogeneic Il-2-stimulated PBL (LAK cells) and TIL was compared. Their activity against a given tumor specimen was similar, but differed against the various other tumor specimens. Thus, the tumor susceptibility and not the cytotoxic potential of the lymphocytes determined the interaction result. An inhibition of lymphocyte penetration was observed in some tumor spheroids, which considerably influenced the resistance to lymphocyte lysis. Apparently it is this inhibition of the migratory capacity of the effector cells by tumor cells that is responsible for tumor resistance to lymphocyte attack.
Ochalek
T
T
Department of Tumor Pathology, Center of Oncology, Cracow, Poland.
von Kleist
S
S
D002450:Cell Communication; D002452:Cell Count; D002478:Cells, Cultured; D002860:Chromium Radioisotopes / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology* / Q000378:metabolism; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000378:metabolism
D002860:Chromium Radioisotopes; D007376:Interleukin-2