Characterization of disulfide linkages in platelet-derived growth factor AA.
Intermolecular and intramolecular disulfide linkages of recombinant human platelet-derived growth factor A chain dimer were determined by chemical methods including selective reduction-alkylation, peptide isolation, or detection of diphenylthiohydantoin derivative of cystine from Edman reactions. Cys-37 and Cys-46 were selectively reduced with reducing agents under native conditions and revealed to be involved in intermolecular bridges. Other disulfide linkages including Cys-10-Cys-54, Cys-43-Cys-91, and Cys-47-Cys-93 form intramolecular bridges. The disulfide structure is homologous to that of platelet-derived growth factor B chain dimer.
Haniu
M
M
Department of Protein Structure, Amgen Inc., Thousand Oaks, California 91320-1789.
Hsieh
P
P
Rohde
M F
MF
Kenney
W C
WC
D000478:Alkylation; D000595:Amino Acid Sequence; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine; D004220:Disulfides / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping; D010982:Platelet-Derived Growth Factor / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014364:Tryptophan / Q000032:analysis
D004220:Disulfides; D010446:Peptide Fragments; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; C075278:platelet-derived growth factor A; D014364:Tryptophan; D003545:Cysteine