title: Thermal injury induces very early production of interleukin-1 alpha in the rat by mechanisms other than endotoxemia.
abstract: BACKGROUND
  Cytokines are putative mediators of thermal injury-induced systemic changes. We studied the effects of thermal injury on cytokine activation in vivo with a sensitive radioimmunoassay specific for rat interleukin-1 alpha (IL-1 alpha).
  
  
  METHODS
  We characterized the organ distribution and expression kinetics of IL-1 alpha in rats submitted to either 20% total body surface area cutaneous burn, muscle burn, or endotoxic shock. Rats were killed at various time points, and liver, lung, spleen, ileum, thymus, kidney, skin, and plasma were harvested. Tissues were homogenized, and the supernates were assayed for rat IL-1 alpha. The assay detection limit was 1.5 ng/gm wet tissue (WT).
  
  
  RESULTS
  Thermal injury induced marked elevations of IL-1 alpha levels in the liver and lung, and maximal levels were reached at 2.5 hours when compared with controls. In the liver mean IL-1 alpha levels in cutaneous burn injury were 16.5 +/- 6.2 ng/gm WT, whereas in sham injury they were 1.7 +/- 0.1 ng/gm WT, p < or = 0.05; in the lung IL-1 alpha levels with cutaneous burn injury were 10.3 +/- 1.3 ng/gm WT, whereas sham injury levels were 1.9 +/- 0.8 ng/gm WT, p < or = 0.002). Levels in all other organs and plasma were below detection limits. Muscle burn injury had similar elevated levels of IL-1 alpha in the liver at 1 hour, indistinguishable from cutaneous burn. In contrast, endotoxin challenge resulted in dramatic elevation of IL-1 alpha levels in all organs tested except for the kidney, whereas the skin maintained its usual large amounts of IL-1 alpha.
  
  
  CONCLUSIONS
  These data indicate that thermal or mechanical injury induce very early and organ-specific association of IL-1 alpha in vivo by mechanisms other than endotoxemia.
authors.affiliation: Surgical Service, Massachusetts General Hospital, Boston.
authors.forename: M
authors.initials: M
authors.lastname: Mester
authors.forename: E A
authors.initials: EA
authors.lastname: Carter
authors.forename: R G
authors.initials: RG
authors.lastname: Tompkins
authors.forename: J A
authors.initials: JA
authors.lastname: Gelfand
authors.forename: C A
authors.initials: CA
authors.lastname: Dinarello
authors.forename: J F
authors.initials: JF
authors.lastname: Burke
authors.forename: B D
authors.initials: BD
authors.lastname: Clark
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism*; D004731:Endotoxins / Q000097:blood*; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004731:Endotoxins; D007375:Interleukin-1