[Detection of a short segment of DNA, responsible for glucocorticoid regulation, in the 5'-flanking region on the murine metallothionein I gene].
Glucocorticoid responsive element (GRE) located -252 to -209 bp upstream of the transcription initiation site of mouse metallothionein I gene (mMT I) was identified in transient gene transfer experiments. This GRE, on its own, was capable of enhancing a reporter CAT gene expression in response to dexamethasone in orientation-independent manner when linked cis to a heterologous HSV TK gene promoter. The duplication of the GRE showed a synergistic effect on dexamethasone induction of the CAT gene. Nevertheless, the GRE located in its natural promoter region of mMT I gene (-330 to +70 bp) was found to provide low if any glucocorticoid induction of linked CAT gene, while showing strong cadmium regulation, comparable to the in vivo level.
Plisov
S Iu
SIu
Merkulova
T I
TI
Shkapenko
A L
AL
D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004247:DNA / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005938:Glucocorticoids / Q000235:genetics* / Q000494:pharmacology; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D005938:Glucocorticoids; D004247:DNA; D008668:Metallothionein; D015500:Chloramphenicol O-Acetyltransferase; D013937:Thymidine Kinase
Vyiavlenie korotkogo uchastka DNK, otvetsvennogo za gliukokortikoidnuiu reguliatsiiu, v blizhneĭ 5'-flankiruiushcheĭ oblasti gena metallotioneina I myshi.