Growth and differentiation of rabbit tracheal outgrowths in primary cultures: influence of substrata.
We show that in rabbit tracheal outgrowths, ciliated cells can express an affinity for the isolectin BSI-B4, known to preferentially bind to basal cells. In multi-layered outgrowths grown on a thick collagen gel, a low percentage of ciliated cells are labelled by BSI-B4. This percentage increases with the aging of cultures. In monolayers developed on thin coatings of collagen or matrigel, a high percentage of ciliated cells show affinity for BSI-B4, even within young cultures.
Bourgeois
C A
CA
Blanquart
C
C
Jeanne
N
N
Moreau
A
A
Marano
F
F
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D002923:Cilia / Q000502:physiology; D004847:Epithelial Cells; D011817:Rabbits; D014132:Trachea / Q000166:cytology*