Decreased activity of 11 beta-hydroxysteroid dehydrogenase in mesenteric arteries of Dahl salt-sensitive rats.
11 beta-Hydroxysteroid dehydrogenase (11 beta-HSD) activity in mesenteric arteries of 4-week- and 8-week-old Dahl rats was determined to evaluate the relationship between glucocorticoids and norepinephrine in hypertension, 11 beta-HSD activity (expressed as the percentage conversion of 3H-corticosterone (B) to 3H-11-dehydrocorticosterone (A)) significantly decreased in 8-week-old Dahl salt-sensitive (DS) rats (11.4 +/- 1.4%) compared with Dahl salt-resistant (DR) rats (15.3 +/- 0.3%) (p < 0.05) when each group was fed a high-sodium chow (7%) for 4 weeks. There were no significant differences in 11 beta-HSD activity in the 4-week-old DS and DR rats. The enhanced response of mesenteric arteries to norepinephrine was observed only in 8-week-old DS rats. Our results suggest that a decreased 11 beta-HSD activity in the vascular walls contributes to the increased response of norepinephrine by local increment of glucocorticoids in DS hypertensive rats.
Takeda
Y
Y
Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
Miyamori
I
I
Yoneda
T
T
Hatakeyama
H
H
Iki
K
K
Takeda
R
R
D042842:11-beta-Hydroxysteroid Dehydrogenases; D000375:Aging; D000450:Aldosterone / Q000097:blood; D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D003345:Corticosterone / Q000097:blood; D006339:Heart Rate; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D006973:Hypertension / Q000235:genetics; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000201:enzymology* / Q000254:growth & development; D024510:Muscle Development; D009131:Muscle, Smooth, Vascular / Q000201:enzymology* / Q000254:growth & development; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012982:Sodium, Dietary; D013599:Systole
D012982:Sodium, Dietary; D000450:Aldosterone; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D003345:Corticosterone