Can adenine nucleotides predict primary nonfunction of the human liver homograft?
Sixty-eight primary liver grafts were analyzed to see whether adenine nucleotides (AN: ATP, ADP, and AMP) or purine catabolites (PC: adenosine, inosine, hypoxanthine, and xanthine) of tissue or effluent can predict primary graft nonfunction. AN, PC, and nicotinamide adenine dinucleotide, oxidized form (NAD+) of the tissue before (pretransplant) and after graft reperfusion (post-transplant) and of the effluent were analyzed. The graft outcome was classified into two groups (group A: successful, n = 64; group B: primary nonfunctioning, n = 4). No significant differences were observed in pretransplant measurements between groups A and B, whereas ATP, ADP, total AN, total AN + total PC (T) and NAD+, in post-transplant tissues, were significantly higher in group A. Xanthine in the effluent was significantly higher in group B than in group A. ATP, ADP, total AN, T, and NAD+ in post-transplant tissue were significantly associated with primary graft nonfunction by logistic regression analysis.
Hamamoto
I
I
Pittsburgh Transplantation Institute, University of Pittsburgh School of Medicine, PA 15213.
Takaya
S
S
Todo
S
S
Bronsther
O
O
Fujita
S
S
Van Gulik
T M
TM
Nakamura
K
K
Irish
W
W
Starzl
T E
TE
D000227:Adenine Nucleotides / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis* / Q000503:physiopathology; D006085:Graft Survival; D006801:Humans; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009243:NAD / Q000378:metabolism; D011687:Purines / Q000378:metabolism; D014184:Transplantation, Homologous
D000227:Adenine Nucleotides; D011687:Purines; D009243:NAD