B2 bradykinin receptors in NG108-15 cells: cDNA cloning and functional expression.
Two distinct cDNAs encoding bradykinin receptors (BKRs) were cloned from NG108-15 neuroblastoma-glioma hybrid cells. One was identical with rat uterus B2 BKR, whereas the other one (mBKR) had 91% amino acid homology to the rat B2 BKR and 82% homology to human B2 BKR. Southern blot analysis and genomic DNA cloning revealed that mBKR is derived from the mouse genome. The mBKR, expressed in Xenopus oocytes and COS-7 cells, produced functional BKRs that exhibited the properties of smooth muscle type B2 BKR. These results suggest that both the rat and mouse B2 BKRs of the smooth muscle type are expressed in NG108-15 cells.
Yokoyama
S
S
Department of Biophysics, Neuroinformation Research Institute, Kanazawa University School of Medicine, Japan.
Kimura
Y
Y
Taketo
M
M
Black
J A
JA
Ransom
B R
BR
Higashida
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001920:Bradykinin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D005910:Glioma; D006801:Humans; D006822:Hybrid Cells; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009865:Oocytes / Q000502:physiology; D016133:Polymerase Chain Reaction; D051381:Rats; D018002:Receptors, Bradykinin / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014407:Tumor Cells, Cultured; D014599:Uterus / Q000378:metabolism; D014981:Xenopus
D017931:DNA Primers; D018076:DNA, Complementary; D018002:Receptors, Bradykinin; D001920:Bradykinin