Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice.
We have established a long-lasting heart allograft tolerance in H-2-incompatible combinations by intravenous injection of 10(8) allogeneic spleen cells followed by the administration of 200 mg/kg of cyclophosphamide (CP). When C57BL/10 mice (B10, H-2b) were primed iv with 1 x 10(8) viable spleen cells from naive BALB/c mice (H-2d) and treated ip with 200 mg/kg CP 2 days later, BALB/c heart allografts were lasting in the recipients for as long as 8 weeks. On the other hand, B10 mice receiving either allogeneic spleen cells or CP rejected the heart allograft within 10 days after transplantation. A direct approach assessing the donor-reactive T cells for frequency and reactivity showed that the donor-reactive T cells were partially deleted and the rest were rendered unresponsive. CP-induced tolerance may be applied for the induction of antigen-specific tolerance to allogeneic heart transplantation.
Matsuura
A
A
Laboratory of Germfree Life, Nagoya University School of Medicine, Japan.
Katsuno
M
M
Suzuki
Y
Y
Ito
T
T
Yasuura
K
K
Abe
T
T
Yoshikai
Y
Y
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D006085:Graft Survival / Q000187:drug effects*; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016019:Survival Analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology; D014184:Transplantation, Homologous
D006183:H-2 Antigens; D003520:Cyclophosphamide