Release and regulation of endogenous adenosine during hemorrhage.
Pretreatment of animals with dilazep, a blocker of adenosine uptake, potentiated the increased concentrations of adenosine in venous blood by 6-fold and raised arterial adenosine concentrations by 3-fold. Pretreatment of animals with erythro-9-)2-hydroxy-3-nonyl)adenine (EHNA), an inhibitor of adenosine deaminase, also potentiated the increased concentrations of adenosine in venous blood by 6-fold and raised arterial adenosine concentrations by 3-fold. Pretreatment with combination of dilazep and EHNA furthermore increased venous adenosine levels by 11 times and arterial adenosine levels by 6 times. These data clearly suggest that hemorrhage induced extensive adenosine release and the released endogenous adenosine was eliminated quickly from plasma by the uptake mechanism and adenosine deaminase.
Zhang
Y L
YL
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Lautt
W W
WW
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000241:Adenosine / Q000097:blood*; D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002415:Cats; D002851:Chromatography, High Pressure Liquid; D004109:Dilazep / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D006470:Hemorrhage / Q000097:blood*; D008297:Male
D058892:Adenosine Deaminase Inhibitors; C010192:9-(2-hydroxy-3-nonyl)adenine; D004109:Dilazep; D000225:Adenine; D000241:Adenosine