title: High-affinity cytotoxic T lymphocytes after non-HLA-sharing blood transfusion--the other side of the coin.
abstract: Previously, we have shown that pretransplantation blood transfusion modulates the T cell repertoire to a great extent. Patients receiving a BT from a donor sharing one HLA haplotype with the patient (HLA-sharing BT) develop CTL nonresponsiveness against cells of the BT donor and show a selective decrease in the usage of T cell receptor V beta families. The present study has focused on the analysis of the T cell repertoire in patients receiving an HLA mismatched (non-HLA-sharing) BT. CTL precursor frequencies were measured against single class I-mismatched antigens in split-well analysis. In addition, blocking studies of CTL-target cell interaction were performed with anti-CD8 monoclonal antibodies. The results demonstrate that non-HLA-sharing BT immunizes and induces the generation of CD8 independent, high-affinity CTL against immunogenic class I-mismatched antigens. Such HLA class I antigens might become nonacceptable mismatches in subsequent organ transplantation.
authors.affiliation: Dept. of Transplantation Immunology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: E
authors.initials: E
authors.lastname: van Twuyver
authors.forename: R J
authors.initials: RJ
authors.lastname: Mooijaart
authors.forename: I J
authors.initials: IJ
authors.lastname: ten Berge
authors.forename: A R
authors.initials: AR
authors.lastname: van der Horst
authors.forename: J M
authors.initials: JM
authors.lastname: Wilmink
authors.forename: F H
authors.initials: FH
authors.lastname: Claas
authors.forename: L P
authors.initials: LP
authors.lastname: de Waal
mesh_terms: D000483:Alleles; D000911:Antibodies, Monoclonal / Q000276:immunology; D001787:Blood Group Incompatibility / Q000209:etiology; D016827:CD8 Antigens / Q000276:immunology; D015536:Down-Regulation; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000502:physiology; D065227:Transfusion Reaction
chemical_list: D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D006680:HLA Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta