The structure of the O-specific chain of Legionella pneumophila serogroup 1 lipopolysaccharide.
The O-polysaccharide chain of Legionella pneumophila Philadelphia strain 1 (serogroup 1) lipopolysaccharide was investigated by means of 1H- and 13C-NMR spectroscopy, and chemical analysis. It was found to consist of an alpha-(2-->4) interlinked homopolymer of a 5-acetamidino-7-acetamido-8-O-acetyl-3,5,7,9-tetradeoxy-nonulos onic acid possessing most likely the D-glycero-L-galacto configuration, representing the first example of an acidic homopolymer of a higher sugar of this class. The ladder-like banding pattern exhibiting small distances between individual bands in the SDS/PAGE is compatible with a monosaccharide repeating unit.
Knirel
Y A
YA
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Germany.
Rietschel
E T
ET
Marre
R
R
Zähringer
U
U
D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D016952:Legionella pneumophila / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure
D008070:Lipopolysaccharides