title: Cloning and expression of cDNA encoding human placental estrogen sulfotransferase.
abstract: Using two oligoprimers derived from the bovine placental estrogen sulfotransferase sequence, we amplified a probe for human placental estrogen sulfotransferase. Using this probe to screen a human placental cDNA library constructed in lambda gt11, we isolated a cDNA clone of 1.3 kb encoding human estrogen sulfotransferase. DNA analysis predicts a protein of 295 amino acids with a calculated molecular weight of 34,199. Alignment of the amino acid sequence with other sulfotransferases indicates that human placental estrogen sulfotransferase shares 68.6, 68.2 and 65.9% similarity with bovine placental, guinea pig adrenocortical, and rat liver estrogen sulfotransferase, respectively. It shows also 95.6, 57.6, 85.3, and 54.2% similarity to human phenol, human DHEA, rat phenol, and rat hydroxysteroid sulfotransferase, respectively. Transfection of expression vectors encoding human estrogen sulfotransferase and dehydroepiandrosterone (DHEA) sulfotransferase in human adrenal adenocarcinoma SW-13 cells indicates that estrogen sulfotransferase transforms estrone more specifically, whereas DHEA sulfotransferase is more specific for DHEA and pregnenolone.
authors.affiliation: Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Quebec, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Bernier
authors.forename: I
authors.initials: I
authors.lastname: Lopez Solache
authors.forename: F
authors.initials: F
authors.lastname: Labrie
authors.forename: V
authors.initials: V
authors.lastname: Luu-The
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008969:Molecular Sequence Data; D011257:Pregnancy Proteins / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D015238:Sulfotransferases / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D011257:Pregnancy Proteins; D011993:Recombinant Fusion Proteins; D015238:Sulfotransferases; C020122:estrone sulfotransferase