Binding of the lipid peroxidation product 4-hydroxynonenal to human polymorphonuclear leukocytes.
4-Hydroxynonenal (HNE) is produced during peroxidation of polyunsaturated fatty acids. It exerts a chemokinetic effect on human polymorphonuclear leukocytes (PMN). Investigations of this mechanism were performed. The results indicate that [3H]-HNE binding to PMN results both in non-specific bonds to the numerous SH groups of the cells and in binding to a saturable, reversible and specific HNE site. Scatchard analysis revealed that this is a single site with an apparent affinity constant of 319 nM and a density of 1.57 pmol (10(6))-1 cells. This specific binding site may be involved in the chemokinetic effect of HNE.
Curzio
M
M
Department of Experimental Medicine and Oncology, University of Turin, Italy.
Ferretti
C
C
Stephens
R J
RJ
Esterbauer
H
H
Dianzani
M U
MU
D000447:Aldehydes / Q000097:blood*; D001665:Binding Sites / Q000502:physiology; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation / Q000502:physiology*; D015394:Molecular Structure; D009504:Neutrophils / Q000378:metabolism*; D011869:Radioligand Assay
D000447:Aldehydes; C027576:4-hydroxy-2-nonenal