[Immunoenzyme method of determining trace amounts of proinsulin in recombinant human insulin].
An enzyme immunoassay (ELISA) for the detection of human proinsulin has been developed based on the interaction of the absorbed proinsulin with the antiserum against the synthetic proinsulin C-peptide. In the presence of high concentrations of insulin, the sensitivity of the assay slightly decreases due to the competitive adsorption of insulin onto the polystyrene carrier with the binding constant 800-1000 times less than that of proinsulin. A method is proposed for the interpretation of ELISA data based on analysis of a weighed dry insulin sample, which enables the detection of a 0.02-0.1% admixture of proinsulin in the chromatographically purified recombinant human insulin.
Britsyna
N S
NS
Prokop'ev
A A
AA
Kulikov
S V
SV
Samartsev
M A
MA
D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera; D007328:Insulin / Q000737:chemistry*; D011384:Proinsulin / Q000032:analysis*; D011994:Recombinant Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D020058:Styrene; D013343:Styrenes
D007106:Immune Sera; D007328:Insulin; D011994:Recombinant Proteins; D013343:Styrenes; D020058:Styrene; D011384:Proinsulin
Immunofermentnyĭ metod opredeleniia sledovykh kolichestv proinsulina v rekombinantnom insuline cheloveka.