[Role of macrophage in presenting antigens of Schistosoma japonicum].
Indirect ELISA was employed to monitor the serum anti-UEA (urea soluble egg antigen of Schistosoma japonicum) antibody level of mice immunized by: a. UEA pulsed macrophage (Mphi+); b. Cultural supernatant of Mphi+; c. paraformaldehyde fixed M phi (P-Mphi) pulsed with UEA; d. Ammonium chloride treated M phi (NH4Cl-Mphi) pulsed with UEA; e. P-Mphi pulsed with trypsin digested UEA (T-UEA); f. NH4Cl-Mphi pulsed with T-UEA. The normal Mphi, its supernatant and the culture media RPMI 1640 acted as the negative control. The results showed: 1. Serum anti-UEA antibody levels of mice immunized by a and b raised markedly, indicating that the immunogenicity of UEA might be kept up after Mphi processing and the antigenic message could be transferred either by the Mphi+ or by its supernatant; 2. Mice immunized by c and d gave similar results, but the anti-UEA antibody level of the former was higher than that of the latter, suggesting that polyformaldehyde could not alter the UEA binding site on the surface of Mphi; 3. In the case of mice immunized by e and f, the antibody levels were much lower than that of mice immunized by c and d, suggesting that UEA binding sites on Mphi surface as well as UEA immunogenicity could be changed by trypsin.
Fang
X H
XH
Department of Parasitology, Sun Yat-Sen University of Medical Sciences, Guangzhou.
Rong
G
G
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D017951:Antigen Presentation / Q000187:drug effects; D000947:Antigens, Helminth / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D012549:Schistosoma japonicum / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000643:Ammonium Chloride; D014357:Trypsin