title: Localization of a human receptor tyrosine kinase (ETK1) to chromosome region 3p11.2.
abstract: We have recently described a human receptor tyrosine kinase (hek) that is expressed by some pre-B and thymic T cell lines, but is not detectable on normal adult human tissues. Gene cloning studies established that hek is a new member of the EPH family of receptor tyrosine kinases. The expression of hek may normally be developmentally regulated and inappropriate expression may contribute to oncogenesis. In the present study, we have used Southern blot analysis of somatic cell hybrids and fluorescence in situ hybridization to localize the hek gene to human chromosome region 3p11.2. Karyotype analysis of the cell lines that over-express hek showed no cytogenetically visible abnormality involving the hek locus.
authors.affiliation: Lions Laboratory, Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.
authors.forename: I P
authors.initials: IP
authors.lastname: Wicks
authors.forename: N M
authors.initials: NM
authors.lastname: Lapsys
authors.forename: E
authors.initials: E
authors.lastname: Baker
authors.forename: L J
authors.initials: LJ
authors.lastname: Campbell
authors.forename: A W
authors.initials: AW
authors.lastname: Boyd
authors.forename: G R
authors.initials: GR
authors.lastname: Sutherland
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D004790:Enzyme Induction; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D036121:Receptor, EphA3; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; D020794:Receptor Protein-Tyrosine Kinases; D036121:Receptor, EphA3