Isolation of three novel POU-domain containing cDNA clones from lactating mouse mammary gland.
Using a PCR-based cloning strategy to identify putative members of the POU-family of transcription factors from genomic mouse DNA or from cDNA derived from lactating or involuting mouse mammary gland 56 POU-domain containing DNA fragments were isolated. Within these 56 clones three cDNA clones seem to be novel putative members of this transcription factor family, referred to as mPit-1R, mBrn-3R and MM-POU-III-A. Expression pattern studies were performed using a reverse transcriptase-mediated PCR approach. For all three different clones distinct developmental and tissue specific transcript levels were obtained, suggesting a tissue specific function of these newly isolated putative members of the POU-family of transcription factors.
Jehn
B
B
Laboratory for Clinical and Experimental Research, University of Bern, Switzerland.
Chicaiza
G
G
Martin
F
F
Jaggi
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D007774:Lactation / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D050809:POU Domain Factors; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013698:Templates, Genetic; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic; D050817:Transcription Factor Pit-1; D018398:Homeodomain Proteins; D050820:Transcription Factor Brn-3B
D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050809:POU Domain Factors; D014157:Transcription Factors; C086884:Pou3f-rs1 protein, mouse; C494185:Pou1f1 protein, mouse; D050817:Transcription Factor Pit-1; C085559:Pou4f2 protein, mouse; D018398:Homeodomain Proteins; D050820:Transcription Factor Brn-3B