[Complementary-addressed photomodification of DNA-targets by arylazide and perfluoroarylazide oligonucleotide derivatives. IV. Photomodification of ss- and ds-DNA-fragments].
A highly efficient sequence-specific photomodification of single stranded (ss) and double stranded (ds) DNA fragments was carried out with hexadecathymidilate derivative, R-p(T)16(R--p-azidotetrafluorobenzamide) and 27-meric DNA fragments as a targets. [formula: see text] The main points of the modification were G7 and G24 for the ss target and G7 and G22 of purine- and pyrimidine-rich strands, respectively, for the ds DNA fragment. The photomodification extent was 60-77% for ss DNA and 10-53% for ds DNA depending on the reaction conditions: it increased in a buffer with a high ionic strength (1.0 M) and at a low temperature (4 degrees C) when the triplexes are more stable.
Levina
A S
AS
Tabatadze
D R
DR
Zarytova
V F
VF
Dobrikov
M I
MI
Gorn
V V
VV
Lokhov
S G
SG
D001386:Azides / Q000737:chemistry*; D001483:Base Sequence; D003080:Cold Temperature; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D009994:Osmolar Concentration; D010777:Photochemistry
D001386:Azides; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D004247:DNA
Komplementarno-adresovannaia fotomodifikatsiia DNK-misheneĭ arilazidnymi i perftorarilazidnymi proizvodnymi oligonukleotidov. IV. Fotomodifikatsiia ss- i ds-DNK-fragmentov.