Competitive in situ hybridization in a mediastinal germ cell tumor.
We performed competitive in situ hybridization in a mediastinal germ cell tumor to identify chromosome 12 aberration(s). DNA from a mouse-human somatic hybrid cell line, containing an i(12p) as unique human material, was labeled and used as a probe. Our results confirm that the chromosome marker, observed in mediastinal germ cell tumor and cytogenetically identified as i(12p), is a true isochromosome of the short arm of chromosome 12.
Aly
M S
MS
Center for Human Genetics, University Hospital Gasthuisberg, Belgium.
Dal Cin
P
P
Jiskoot
P
P
Deneffe
G
G
Marynen
P
P
Van den Berghe
H
H
D000293:Adolescent; D000818:Animals; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D025063:Chromosome Disorders; D002881:Chromosomes, Human, Pair 12; D018237:Germinoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008479:Mediastinal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D051379:Mice