Cytogenetic effects of phosphine inhalation by rodents. I: Acute 6-hour exposure of mice.
Phosphine (PH3) is a highly toxic grain fumigant that can be produced from the reaction of metal phosphides with water. To determine the in vivo cytogenetic effects of inhalation of PH3, male CD-1 mice were exposed to either 0, 5, 10, or 15 ppm target concentrations of PH3 for 6 hr. Twenty hours after the termination of exposure, the spleens of the mice were removed, macerated, and the splenocytes cultured for analyses of sister chromatid exchanges, chromosome aberrations, and micronuclei in cytochalasin B-induced binucleated cells. In addition, bone marrow smears were made for the analysis of micronuclei in polychromatic erythrocytes. No increase in any of the cytogenetic endpoints was found at any of the concentrations examined. The only statistically significant response was a concentration-related slowing of the cell cycle in the splenocytes.
Kligerman
A D
AD
Genetic Toxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina.
Bryant
M F
MF
Doerr
C L
CL
Erexson
G L
GL
Kwanyuen
P
P
McGee
J K
JK
D000280:Administration, Inhalation; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D010720:Phosphines / Q000008:administration & dosage / Q000633:toxicity*; D012854:Sister Chromatid Exchange; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013997:Time Factors
D007306:Insecticides; D009153:Mutagens; D010720:Phosphines; C044646:phosphine