Microwave immunoincubations using coverplate units.
Recently, the Coverplate immunostaining method was introduced. This system allows easy handling of the slides and is suitable for immunoincubations for a variety of antigens at the same time on consecutive sections, without having to apply droplets of the individual primary antibodies on the sections. In this study, temperature rise inside the Coverplate units during microwave exposure is investigated. The most suitable conditions for microwave immunoincubations are determined. Immunoincubations with the Coverplate unit are conducted in the microwave oven and the results evaluated.
Douwes Dekker
P B
PB
Leiden Cytology and Pathology Laboratory, The Netherlands.
Boon
M E
ME
Vardaxis
N J
NJ
D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D008872:Microwaves; D009363:Neoplasm Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012867:Skin / Q000737:chemistry / Q000528:radiation effects / Q000648:ultrastructure; D012878:Skin Neoplasms / Q000737:chemistry / Q000648:ultrastructure; D013696:Temperature; D014867:Water
D009363:Neoplasm Proteins; D011506:Proteins; D014867:Water