Spectral alterations in Rhodobacter capsulatus mutants with site-directed changes in the bacteriochlorophyll-binding site of the B880 light-harvesting complex.
Site-directed mutagenesis has suggested that conserved histidine and alanine residues in the alpha-subunit of the B880 (LHI) antenna complex of Rhodobacter capsulatus (alpha His32 and alpha Ala28) form part of the bacteriochlorophyll binding site (Bylina, E.J., Robles, S.J. and Youvan, D.C. (1988) Isr. J. Chem. 28, 73-78). Spectroscopic characterization of chromatophores from alpha Ala28 mutants at 77 K revealed: (i) red shifts in B880 absorption and emission maxima of approximately 6 and 10 nm, respectively, with a serine exchange; (ii) red shifts of 3 nm with a glycine exchange; (iii) and no significant shifts with a cysteine exchange, despite a reduction of approximately 50% in B880 level. The strains with the serine and glycine exchanges showed characteristic fluorescence polarization increases over the red-edge of the B880 band, suggesting that the absorption red shifts arose from altered pigment-protein interactions rather than from increased oligomerization states that would be expected to show markedly diminished and red shifted rises in polarization (Westerhuis, W.H.J., Farchaus, J.W. and Niederman, R.A. (1993) Photochem. Photobiol. 58, 460-463). Excitation spectra of strains with alpha His32 to glutamine and alpha Ala28 to histidine exchanges, thought to be depleted in B880, revealed low levels of a 'pseudo-B880' complex with blue-shifted maxima and fluorescence polarization rises; when excited directly into this component, the former strain showed an emission spectrum similar to that of B880. An essentially wild-type electrochromic carotenoid response was observed only in the B880-containing mutants, since membranes isolated from the B880-depleted strains exhibited an increased permeability to ions.
Olivera
L M
LM
Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08855-1059.
Westerhuis
W H
WH
Niederman
R A
RA
D000595:Amino Acid Sequence; D001429:Bacteriochlorophylls / Q000737:chemistry / Q000235:genetics*; D001665:Binding Sites; D016384:Consensus Sequence; D005454:Fluorescence Polarization; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000235:genetics*; D016354:Rhodobacter capsulatus / Q000235:genetics*; D013053:Spectrophotometry
D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins