Isolation and sequence of the t-RNA ligase-encoding gene of Candida albicans.
The gene encoding tRNA ligase from Candida albicans was isolated from a genomic library by complementation of a Saccharomyces cerevisiae strain containing a disrupted structural gene, RLG1, encoding tRNA ligase. The cloned gene also complements a temperature-sensitive allele of RLG1. Sequence analysis revealed a single 2499-nt coding region. The gene encodes a protein of 833 amino acids that is 42% identical to S. cerevisiae tRNA ligase. Hybridization to chromosomes of C. albicans separated by pulsed-field gel electrophoresis located the gene to chromosome 1, the smallest C. albicans chromosome.
Baymiller
J
J
Department of Microbial Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.
Jennings
S
S
Kienzle
B
B
Gorman
J A
JA
Kelly
R
R
McCullough
J E
JE
D000595:Amino Acid Sequence; D001483:Base Sequence; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics*; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000302:isolation & purification; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D011130:RNA Ligase (ATP) / Q000235:genetics*; D015183:Restriction Mapping
D004271:DNA, Fungal; D011130:RNA Ligase (ATP)