Gender-related differences of mouse liver D-aspartate oxidase in the activity and response to administration of D-aspartate and peroxisome proliferators.
1. The hepatic D-aspartate oxidase activity was found to be higher in female ddY and ICR mice than in their male counterparts. On the contrary, the free D-aspartate content in the liver was lower in female mice than in male mice, suggesting that D-aspartate is actually metabolized by D-aspartate oxidase in vivo. 2. Oral administration of D-aspartate to the animals increased the hepatic D-aspartate oxidase activity 2-3 fold in both genders without any significant difference in the rate of the increase between the genders. 3. Several peroxisomal enzyme activities other than D-aspartate oxidase examined were not affected by this treatment. 4. Experiments in vitro suggested that the increase in the D-aspartate activity might be explained in part by stabilization of the enzyme by D-aspartate. 5. The administration of clofibrate, a peroxisome proliferator, to male mice, increased the hepatic D-aspartate oxidase activity with a significant simultaneous decrease of D-aspartate content in the liver, in agreement with a possible role of the enzyme in vivo. 6. On the other hand, the administration of clofibrate or dehydroepiandrosterone to female mice decreased the D-aspartate oxidase activity. 7. The peroxisome proliferators were suggested to act to eliminate the gender difference of hepatic D-aspartate oxidase activity in mice.
Nagasaki
H
H
Department of Biochemistry, Kyoto Prefectural University of Medicine, Japan.
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology*; D002994:Clofibrate / Q000494:pharmacology; D050804:D-Aspartate Oxidase; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D008830:Microbodies / Q000187:drug effects*; D012727:Sex Characteristics
D001224:Aspartic Acid; D003687:Dehydroepiandrosterone; D000594:Amino Acid Oxidoreductases; D050804:D-Aspartate Oxidase; C494102:DDO protein, human; D002994:Clofibrate