A single promoter sequence recognized by a newly identified alternate sigma factor directs expression of pathogenicity and host range determinants in Pseudomonas syringae.
A conserved sequence motif associated with transcription of avr genes was identified in the promoter regions of six Pseudomonas syringae pv. syringae Pss61 hrp operons. A 34-bp fragment carrying this motif was cloned from the HrpZ promoter region and was shown to confer HrpL-dependent promoter activity. Expression of pathogenicity and host range determinants in P. syringae strains is thus directed by the apparent alternate sigma factor HrpL.
Xiao
Y
Y
Department of Botany, University of Maryland, College Park 20742.
Hutcheson
S W
SW
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D011549:Pseudomonas / Q000235:genetics* / Q000472:pathogenicity; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012808:Sigma Factor / Q000378:metabolism*; D013045:Species Specificity; D014158:Transcription, Genetic / Q000187:drug effects
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C085855:HrpL protein, Pseudomonas syringae; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; C087295:HrpZ protein, Pseudomonas syringae