Catabolism of the tetrapeptide N-Ac-Ser-Asp-Lys-Pro (AcSDKP), an inhibitor of hematopoietic stem cell (CFU-S) proliferation, following in vitro incubation with hematopoietic tissues from normal and leukemic mice.
The comparative degradation of N-Ac-Ser-Asp-Lys-Pro (AcSDKP), a negative regulator controlling the proliferation of the hematopoietic pluripotent stem cell, was investigated following incubation with plasma, bone marrow and spleen cells from normal mice and mice bearing a transplantable myeloid leukemia. Using the tetrapeptide, specifically radiolabelled in the lysyl residue, degradation of [3H]AcSDKP was followed by measurement of [3H]Lys formation resulting from its catabolism. It was shown that already after 1 h the degradation of AcSDKP in plasma from leukemic mice was higher compared to that following incubation in plasma from normal mice, whereas incubation with bone marrow cells exhibits a small difference only after 4 hours incubation. However, no increase of AcSDKP catabolic activity was observed following incubation with spleen cells from leukemic animals when compared with incubation of normal spleen cells.
Wdzieczak-Bakala
J
J
Institut de chimie des substances naturelles, Centre national de la recherche scientifique, Gif-sur-Yvette, France.
Grillon
C
C
Robinson
S
S
Riches
A
A
Carde
P
P
Lenfant
M
M
D000818:Animals; D001854:Bone Marrow Cells; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D007942:Leukemia, Experimental / Q000097:blood / Q000378:metabolism*; D051379:Mice; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D009842:Oligopeptides; C058504:goralatide