Induction of the small stress protein, hsp25, in Ehrlich ascites carcinoma cells by anticancer drugs.
Treatment of in vitro cultured Ehrlich ascites carcinoma cells with cisplatin, daunomycin, doxorubicin, cytosine arabinoside, 3'-fluorodeoxythymidine, colchicine and vincristine in cytostatically effective concentrations results in significantly increased levels of the small stress protein, hsp25, as analyzed by immunoblotting. However, no induction of hsp25 could be detected after treatment of the tumour cells with 5-fluorouracil, aminopterin, amethopterin, mithramycin and cyclophosphamide. None of these cytostatic drugs induces hsp70.
Bielka
H
H
Max-Delbrück-Center for Molecular Medicine, Berlin-Buch, Germany.
Hoinkis
G
G
Oesterreich
S
S
Stahl
J
J
Benndorf
R
R
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins