Suitability of autoclaved tap water for preparation of ELISA reagents and washing buffer.
The suitability of autoclaved tap water for the preparation of ELISA reagents and washing buffer was compared with that of ultrapure water, in a standard indirect ELISA for the detection of antibodies to pseudorabies virus (PRV). The performance of the assay, using autoclaved tap water (AT-ELISA) compared favourably to that of the standard assay, using ultrapure water (UP-ELISA) in detecting anti-PRV antibodies in sequential serum samples from a pig experimentally infected with PRV. While both the UP-ELISA and AT-ELISA proved reliable in detecting anti-PRV antibodies in a coded proficiency serum panel (n = 60), the AT-ELISA detected fewer positive sera than the UP-ELISA in evaluating a limited number (n = 80) of field samples. The results suggest that autoclaved tap water may be substituted for ultrapure water for the preparation of ELISA reagents when or where ultrapure water may not be available.
Afshar
A
A
Animal Diseases Research Institute, Agriculture Canada, Nepean, Ontario.
Zhao
X
X
Heckert
R A
RA
Trotter
H C
HC
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D011558:Herpesvirus 1, Suid / Q000276:immunology; D006358:Hot Temperature; D013242:Sterilization / Q000379:methods*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology; D014867:Water
D000914:Antibodies, Viral; D014867:Water