Detection of Borna disease virus RNA in naturally infected animals by a nested polymerase chain reaction.
Borna disease virus in naturally infected horses, a donkey and sheep was detected for the first time by amplification of viral RNA using PCR. In contrast to a control group of healthy horses, brain tissue was positive by this assay in all animals with neurological symptoms. The use of a second round of PCR with nested primers following Southern hybridization confirmed the specificity and increased the sensitivity of the test. Comparison with conventional methods recommends this technique for monitoring of BDV infections at a molecular level.
Zimmermann
W
W
Institut für Virologie, Freie Universität Berlin, Germany.
Dürrwald
R
R
Ludwig
H
H
D000818:Animals; D001483:Base Sequence; D001890:Borna Disease / Q000382:microbiology*; D001891:Borna disease virus / Q000302:isolation & purification*; D001921:Brain / Q000382:microbiology; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D014773:Virology / Q000379:methods
D012367:RNA, Viral