title: Regulation of TFIIH ATPase and kinase activities by TFIIE during active initiation complex formation.
abstract: The general transcription factor TFIIE, together with other general transcription factors, is essential for transcription initiation by RNA polymerase II. TFIIE stimulates the TFIIH-dependent kinase activity that phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II, and possesses a helicase activity. Here we show that human TFIIH has DNA-dependent ATPase activity and we characterize the stimulatory effect of TFIIE on both the ATPase and kinase activities. We demonstrate that extensive phosphorylation of RNA polymerase II occurs in a TFIIE-dependent manner in both the absence and presence of DNA but, in the latter case, only at a late stage of preinitiation complex assembly. We also show that TFIIH specifically phosphorylates three general transcription factors, human TFIID tau (TBP), TFIIE-alpha and TFIIF-alpha (RAP74).
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkuma
authors.forename: R G
authors.initials: RG
authors.lastname: Roeder
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004265:DNA Helicases; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000378:metabolism*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
chemical_list: D009842:Oligopeptides; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C066644:transcription factor TFIIE; C078883:tyrosyl-seryl-prolyl-threonyl-seryl-prolyl-seryl-tyrosine; D051758:Transcription Factor TFIIH; D011494:Protein Kinases; C060137:carboxy-terminal domain kinase; D012321:DNA-Directed RNA Polymerases; D000251:Adenosine Triphosphatases; D004265:DNA Helicases