title: gyrB mutations in coumermycin A1-resistant Borrelia burgdorferi.
abstract: We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.
authors.affiliation: Laboratory of Vectors and Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
authors.forename: D S
authors.initials: DS
authors.lastname: Samuels
authors.forename: R T
authors.initials: RT
authors.lastname: Marconi
authors.forename: W M
authors.initials: WM
authors.lastname: Huang
authors.forename: C F
authors.initials: CF
authors.lastname: Garon
mesh_terms: D000595:Amino Acid Sequence; D049933:Aminocoumarins; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D003374:Coumarins / Q000494:pharmacology; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D008969:Molecular Sequence Data; D017354:Point Mutation; D017386:Sequence Homology, Amino Acid
chemical_list: D049933:Aminocoumarins; D000900:Anti-Bacterial Agents; D003374:Coumarins; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II; C004628:coumermycin