Effects of low-sodium, high-potassium dietary intake on cochlear lateral wall Na+,K(+)-ATPase.
The effect of a low Na+, high K+ diet on Na+,K(+)-ATPase levels in cochlear lateral wall tissues was investigated in laboratory rats by using an enzyme-linked immunosorbent assay. The low Na+, high K+ diet induced high aldosterone plasma levels in the animals as well as changes in plasma cation levels. Animals that received a low Na+, high K+ diet demonstrated a statistically significant (97%) increase in Na+,K(+)-ATPase levels in the stria vascularis when compared to animals that received a control diet. This increase in strial Na+,K(+)-ATPase levels was blocked only 70% by administration of the aldosterone antagonist, spironolactone. Findings therefore indicate that strial Na+,K(+)-ATPase may be modulated by both aldosterone and Na+,K+ plasma levels. Na+,K(+)-ATPase levels in the spiral ligament were not affected by the experimental treatment. These findings suggest that spiral ligament Na+,K(+)-ATPase levels may be regulated by factors other than aldosterone and Na+,K+ plasma levels. This study provides further insight into the mechanisms of the beneficial effects of salt restriction and potassium loading in patients with Méniére's disease.
ten Cate
W J
WJ
Department of Anatomy, College of Medicine, University of Florida, Gainesville 32610.
Curtis
L M
LM
Rarey
K E
KE
D000450:Aldosterone / Q000097:blood; D000818:Animals; D003051:Cochlea / Q000187:drug effects* / Q000201:enzymology*; D003053:Cochlear Duct / Q000187:drug effects / Q000201:enzymology; D004039:Diet, Sodium-Restricted; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D011188:Potassium / Q000097:blood / Q000652:urine; D017419:Potassium, Dietary / Q000097:blood / Q000494:pharmacology* / Q000652:urine; D051381:Rats; D012964:Sodium / Q000097:blood / Q000652:urine; D012982:Sodium, Dietary / Q000097:blood / Q000494:pharmacology* / Q000652:urine; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000037:antagonists & inhibitors; D013148:Spironolactone / Q000097:blood / Q000494:pharmacology; D013316:Stria Vascularis / Q000187:drug effects / Q000201:enzymology
D017419:Potassium, Dietary; D012982:Sodium, Dietary; D013148:Spironolactone; D000450:Aldosterone; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium