Simultaneous measurement of acetylcholine and monoamines by two serial on-line microdialysis systems: effects of methamphetamine on neurotransmitters release from the striatum of freely moving rats.
A rapid and sensitive set-up consisting of a microbore HPLC with electrochemical detection coupled with two serial on-line microdialysis systems was described to simultaneously measure endogenous levels of acetylcholine (ACh), choline (Ch), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 3-methoxytyramine (3-MT), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) in freely moving rats. The validity of the present procedure is demonstrated by analyzing the effect of methamphetamine (2 mg/kg, s.c.) on the increase release of DA, 3-MT, 5-HT, and ACh but without effect in Ch, DOPAC, HVA and 5-HIAA from the striatum of freely moving rat.
Tsai
T H
TH
National Research Institute of Chinese Medicine, Taipei, Taiwan.
Chen
C F
CF
D000109:Acetylcholine / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis / Q000378:metabolism*; D002794:Choline / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D017551:Microdialysis; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015306:Biogenic Monoamines; D008694:Methamphetamine; D002794:Choline; D000109:Acetylcholine