title: Structures associated with the expression of rabies virus structural genes in insect cells.
abstract: When rabies virus structural genes were expressed in insect cells, major observed alterations were a high level of cytoplasmic vacuolization caused by the matrix protein M2 and the glycoprotein G. Ring-like structures, 16 nm in diameter, were observed in cell-free extracts from insect cells that expressed the N protein alone. Hexagonally shaped structures, 16-20 nm in diameter, and regular lattice aggregates of the same structures appeared on co-expression of N and M1 proteins. Co-expression of the four structural proteins led to the formation of cell surface blebs containing the structures corresponding to N and M1 proteins.
authors.affiliation: NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
authors.forename: R M
authors.initials: RM
authors.lastname: Pinto
authors.forename: A
authors.initials: A
authors.lastname: Bosch
authors.forename: D H
authors.initials: DH
authors.lastname: Bishop
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics; D002460:Cell Line; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D015870:Gene Expression; D005814:Genes, Viral; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D009036:Moths; D017924:Nucleopolyhedroviruses / Q000235:genetics; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins; C064951:glycoprotein G, Rabies virus