[Analysis of the protein/polypeptide in Culex pipiens pallens (Diptera:Culicidae) during hibernation by two-dimensional gel electrophoresis].
Analysis on the protein/polypeptide in Culex pipiens pallens (Diptera: Culicidae) caught from the resting or overwintering places during the 3rd ten-day of September, the 2nd ten-day of December and the 1st ten-day of March in next year in Zhengzhou (34 degrees 43' N, 113 degrees 39' E) during hibernation by two-dimensional gel electrophoresis indicated that there were 153, 118 and 169 protein/polypeptide spots in unoverwintering, overwintering and recovering mosquitoes respectively. The molecular weight (MW) of most of the spots was less than 43.0 kDa, with isoelectric points (pI) between 5.65-7.34. The comparisons between each two types of mosquitoes during hibernation revealed that the variant rate of protein/polypeptide spots between overwintering and recovering mosquitoes (31.01%) was significantly higher than that between unoverwintering and recovering females (21.12%) (P < 0.05). The variant spots were 13 in unoverwintering, 7 in overwintering and 27 in recovering mosquitoes respectively. The comparisons among three types of mosquitoes during hibernation showed that the variant rate was significantly higher in recovering females (15.98%) than in unoverwintering (8.50%) (P < 0.05) and overwintering females (5.93%) (P < 0.01). The total variant rate of protein/polypeptide spots of all these three types of mosquitoes was 10.68%. Because the blood meal of all the mosquitoes used for homogenization was at Sella 1 (empty), and the ovary development was under Christophers II (resting stage), the experimental results were not affected by blood meal and the later ovary development (Christophers II-V).
Su
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Department of Parasitology, Henan Medical University, Zhengzhou.
Ye
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Su
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Zhao
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D000818:Animals; D003465:Culex / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006605:Hibernation; D011506:Proteins / Q000378:metabolism*
D011506:Proteins