High resolution mapping of overlapping cosmids by fluorescence in situ hybridization.
The constituents of two cosmid contigs were analyzed by high resolution mapping using two-color fluorescence in situ hybridization (FISH) to extended DNA molecules. Samples were prepared by lysing the nuclei in situ followed by histone depletion. This treatment results in elongate DNA filaments appropriate for high resolution mapping. The hybridization signals appeared as a strong of fluorescent spots separated by non-fluorescing gaps. Probe-specific features of the hybridization patterns were detected and some of the non-fluorescing gaps were found to represent regions of repetitive DNA suppressed during hybridization.
Houseal
T W
TW
Integrated Genetics, Framingham, Massachusetts 01701-9322.
Dackowski
W R
WR
Landes
G M
GM
Klinger
K W
KW
D000650:Amnion / Q000737:chemistry / Q000166:cytology; D015139:Blotting, Southern; D002478:Cells, Cultured; D002874:Chromosome Mapping; D003360:Cosmids; D004247:DNA / Q000032:analysis / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence
D004247:DNA