Acyl-CoA-binding protein (ACBP) can mediate intermembrane acyl-CoA transport and donate acyl-CoA for beta-oxidation and glycerolipid synthesis.
The dissociation constants for octanoyl-CoA, dodecanoyl-CoA and hexadecanoyl-CoA binding to acyl-CoA-binding protein (ACBP) were determined by using titration microcalorimetry. The KD values obtained, (0.24 +/- 0.02) x 10(-6) M, (0.65 +/- 0.2) x 10(-8) M and (0.45 +/- 0.2) x 10(-13) M respectively, were much lower than expected. ACBP was able to extract hexadecanoyl-CoA from phosphatidylcholine membranes immobilized on a nitrocellulose membrane. The acyl-CoA/ACBP complex formed was able to transport acyl-CoA to mitochondria or microsomes in suspension, or to microsomes immobilized on a nitrocellulose membrane, and to donate them to beta-oxidation or glycerolipid synthesis in mitochondria or microsomes, respectively.
Rasmussen
J T
JT
Institute of Biochemistry, Odense University, Denmark.
Faergeman
N J
NJ
Kristiansen
K
K
Knudsen
J
J
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002151:Calorimetry; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D026261:Diazepam Binding Inhibitor; D005260:Female; D005989:Glycerides / Q000096:biosynthesis*; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D011817:Rabbits; D051381:Rats
D000214:Acyl Coenzyme A; D002352:Carrier Proteins; D026261:Diazepam Binding Inhibitor; D005989:Glycerides