Cyclic AMP causes differentiation and decreases the expression of neutral glycosphingolipids in cell cultures derived from a malignant glioma.
Cultures derived from a malignant glioma (U-87 MG) were treated with 3 mM dibutyryl cAMP. The treatment resulted in morphological differentiation of the cultures and a decrease in cell proliferation. Biochemically, dibutyryl cAMP treatment caused a general reduction in the concentration of neutral glycosphingolipids in the U-87 MG cells. The concentration of individual neutral glycosphingolipids in the untreated cells was 1.8- to 3.0-fold higher than in cells treated for 72 h with 3 mM dibutyryl cAMP. Cells were labeled with [3H]galactose to monitor synthesis of the neutral glycosphingolipids. Decreased synthesis was noted in cells treated with dibutyryl cAMP as compared with untreated cells as indicated by decreased uptake of [3H]galactose label. The ganglioside composition of the cells was essentially unchanged after dibutyryl cAMP treatment.
Hoffman
L M
LM
Neuroscience Center, Kingsbrook Jewish Medical Center, Brooklyn, NY 11203.
Brooks
S E
SE
Stein
M R
MR
Schneck
L
L
D003994:Bucladesine / Q000494:pharmacology*; D002453:Cell Cycle; D002454:Cell Differentiation; D005732:Gangliosides / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects; D005910:Glioma / Q000378:metabolism*; D006028:Glycosphingolipids / Q000096:biosynthesis*; D006801:Humans; D008940:Mitotic Index / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects
D005732:Gangliosides; D006028:Glycosphingolipids; D003994:Bucladesine