[Effect of GDP and carboxyatractylate on potassium ion transport in brown fat mitochondria].
It has been shown that the increase of the membrane potential in uncoupled brown fat mitochondria after addition of 1-2 mM GDP to a medium containing potassium acetate is accompanied by swelling of mitochondria which occurs in two phases. The first swelling phase is due to the electrogenic transport of K+ ions, while the second one is activated by carboxyatractylate and carnitine. The swelling is practically completely inhibited by a further rise of the GDP concentration irrespective of high values of the membrane potential. Unlike K+ transport, the potential-dependent transport of Na+ ions occurs more rapidly, is monophasic and insensitive to carboxyatractylate and carnitine. It is suggested that regulation of K+ ion transport in brown fat mitochondria is mediated by the action of nucleotides and fatty acid esters on the uncoupling protein and ADP/ATP antiporter.
Fedotcheva
N I
NI
Skarga
Iu Iu
IuIu
Foĭgel'
A G
AG
D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives* / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D006153:Guanosine Diphosphate / Q000494:pharmacology*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D008933:Mitochondrial Swelling; D011188:Potassium / Q000378:metabolism*; D012589:Sciuridae
D006153:Guanosine Diphosphate; D001278:Atractyloside; D011188:Potassium; C003853:carboxyatractyloside
Vliianie GDP i karboksiatraktilata na transport ionov kaliia v mitokhondriiakh burogo zhira.