The ambiguous effect of ascorbic acid on chromate induced proteinuria in rats.
The influence of ascorbic acid (AA, 5 g/kg body weight) on chromate (Cr, 10 mg/kg) induced proteinuria, which is a sensitive parameter of its nephrotoxicity, was investigated in adult female Wistar rats. The concentrations of Cr and ascorbic acid (AA) were determined in renal tissue. Cr nephrotoxicity is related to its intracellular reduction from Cr(VI) to Cr(III). Proteinuria was completely prevented by enhancement of extracellular reduction of Cr(VI) to Cr(III) followed by rapid renal excretion when Cr and AA were given concomitantly. With an interval up to 1 h between Cr and AA, proteinuria was decreased probably by the radical scavenging function of AA. At an interval of 3 h AA enhanced Cr toxicity by increased intracellular Cr reduction. If the interval was increased to 5 h or if Cr was given 24 h after AA, no influence of AA could be detected. Our results confirm that AA is a very effective reductant of Cr which can influence Cr nephrotoxicity in very high concentrations. It depends on the interval between Cr and AA administration whether or not there is a beneficial effect of AA in Cr nephrotoxicity.
Appenroth
D
D
Institute of Pharmacology and Toxicology, Friedrich Schiller University, Jena, Germany.
Winnefeld
K
K
Schröter
H
H
Rost
M
M
D000818:Animals; D001205:Ascorbic Acid / Q000493:pharmacokinetics / Q000494:pharmacology*; D002840:Chromates / Q000493:pharmacokinetics / Q000633:toxicity*; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced; D011507:Proteinuria / Q000139:chemically induced*; D051381:Rats; D017208:Rats, Wistar
D002840:Chromates; D001205:Ascorbic Acid