title: Canine distemper virus replication in cells on microcarriers.
abstract: Chick embryo and Vero cells were grown on Gelaspher M gelatin microcarriers in suspension culture. The microcarriers had no adverse effects on cell morphology and growth. Microcarrier cell cultures were used for large-scale production of canine distemper virus. Virus yields (TCID50 per ml) were more than 10-times higher as compared to stationary cell culture.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava.
authors.forename: J
authors.initials: J
authors.lastname: Lesko
authors.forename: P
authors.initials: P
authors.lastname: Veber
authors.forename: E
authors.initials: E
authors.lastname: Duseková
mesh_terms: D000818:Animals; D001709:Biotechnology; D002478:Cells, Cultured; D002642:Chick Embryo; D004217:Distemper Virus, Canine / Q000254:growth & development / Q000502:physiology*; D005780:Gelatin; D010316:Particle Size; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
chemical_list: D005780:Gelatin