Immunogenicity in cows of Staphylococcus aureus type 5 capsular polysaccharide-ovalbumin conjugate.
Six dairy cows were immunized subcutaneously with purified type 5 capsular polysaccharide (CP5) of Staphylococcus aureus or CP5-ovalbumin conjugate in Freund's incomplete adjuvant. The CP5 antibodies elicited were measured in sera and analysed with regard to isotypes by enzyme-linked immunosorbent assay. At the doses tested, the purified CP5 did not induce a humoral response in the cows. Immunization of two cows with the CP5-ovalbumin conjugate elicited a CP5 antibody response mainly of the IgG2 isotype, which culminated 4 weeks later. A second injection of conjugate, 3 months after the first one, resulted in a rapid and durable anti-CP5 response without exceeding the first antibody peak value. Intramammary infusion of purified CP5 failed to provoke an inflammatory response in the milk of the immunized cows. In contrast, a marked recruitment of cells was recorded in the milk of the sensitized cows after intramammary infusion of ovalbumin. These results demonstrate that injection of CP5-protein carrier conjugate in cows entails both antibody response against CP5 and carrier-specific recruitment of cells in milk of immunized animals.
Gilbert
F B
FB
Laboratoire de Pathologie Infectieuse et d'Immunologie, Institut National de la Recherche Agronomique, Nouzilly, France.
Poutrel
B
B
Sutra
L
L
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D016667:Bacterial Capsules; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005620:Freund's Adjuvant; D007263:Infusions, Parenteral / Q000662:veterinary; D007279:Injections, Subcutaneous / Q000662:veterinary; D008321:Mammary Glands, Animal / Q000276:immunology; D008892:Milk / Q000166:cytology; D010047:Ovalbumin / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*
D000907:Antibodies, Bacterial; D011135:Polysaccharides, Bacterial; C054106:capsular polysaccharide, type 5, Staphylococcus aureus; D010047:Ovalbumin; D005620:Freund's Adjuvant