title: Use of lac gene fusions in the analysis of Acholeplasma upstream gene regulatory sequences.
abstract: The objective of this study was to develop a means of identifying and analyzing mycoplasma gene regulatory elements. Analysis of Acholeplasma oculi ISM1499 transcriptional sequences was accomplished using a promoterless lacZ reporter gene and an integrative vector strategy. Seven promoter-containing fragments were identified, levels of beta-galactosidase were determined, and transcriptional start sites were mapped.
authors.affiliation: Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.
authors.forename: K L
authors.initials: KL
authors.lastname: Knudtson
authors.forename: F C
authors.initials: FC
authors.lastname: Minion
mesh_terms: D000127:Acholeplasma / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D007763:Lac Operon; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic