Isolation of thyroid peroxidase from patients with Graves' disease and comparison with animal peroxidases.
1. Human thyroid peroxidase (TPO) was isolated from 280-640 g of pooled thyroid tissue resected from patients with Graves' disease. 2. Isolation was performed by an improved and simplified method. 3. The Reinheit Zahl (A412/A280) of the final preparations was in the range of 0.16-0.32. 4. The spectroscopic and enzymatic properties of Graves' TPO were compared with those of porcine TPO and bovine LPO, revealing closer resemblance to the former. 5. Graves' TPO may provide a useful substitute for normal TPO, which is very difficult to isolate.
Hosoya
T
T
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Yoshimura
H
H
Naito
N
N
Suzuki
N
N
Sato
K
K
Takamatsu
J
J
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006111:Graves Disease / Q000201:enzymology*; D006139:Guaiacol / Q000737:chemistry; D006801:Humans; D007453:Iodide Peroxidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D007454:Iodides / Q000737:chemistry; D007526:Isoelectric Point; D007700:Kinetics; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013053:Spectrophotometry; D013552:Swine; D013961:Thyroid Gland / Q000201:enzymology*
D007454:Iodides; D006139:Guaiacol; D007453:Iodide Peroxidase