[Intracerebroventricular infusions of fibroblast growth factor and its fragments suppress nocturnal feeding in rats].
Acidic fibroblast growth factor1-140 (aFGF1-140), basic FGF1-146 (bFGF1-146) and several aFGF fragments were injected into the 3rd ventricle of rats to investigate their acute effects on the nocturnal food intake. Infusions of aFGF1-140 and bFGF1-146 immediately before the onset of darkness significantly suppressed the food intake for 12 hrs in a dose-dependent manner. The potency of aFGF1-140 to inhibit the feeding was 1.5 times greater than that of bFGF1-146. While an infusion of aFGF114-140 (a C-terminal fragment) had no effect on the feeding, that of aFGF1-15 (a N-terminal fragment) suppressed the food intake, although its potency corresponded to about 1/80 of the same molar concentration of aFGF1-140. An infusion of the other aFGF fragments containing N-terminals (aFGF1-29, aFGF1-20) or aFGF9-29 had no effect on the food intake. However, [16Ala]aFGF1-29, in which the cysteine residue at the position 16 was replaced with alanine, significantly depressed the food intake. Its potency was about 1/20 that of aFGF1-140. These results suggest that aFGF1-140 and bFGF1-146 participate in the central regulation of food intake, and that the amino-terminal portion of aFGF is critical for its action on feeding regulation.
Li
A J
AJ
Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka.
D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior / Q000187:drug effects*; D005346:Fibroblast Growth Factors / Q000008:administration & dosage / Q000494:pharmacology*; D007276:Injections, Intraventricular; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005346:Fibroblast Growth Factors