title: Distribution of tetracycline resistance determinants among gram-negative bacteria isolated from polluted and unpolluted marine sediments.
abstract: Tetracycline-resistant gram-negative bacteria were isolated from four different marine sediments in Scandinavia and analyzed with DNA probes for the determinant classes A to E. Colony hybridizations of 429 isolates revealed that class E is the dominating resistance determinant in these marine sediments. Comparison of fecally polluted and unpolluted sediments showed few determinant classes in unpolluted sediment and a complex composition of several determinant classes in polluted sediment. Total DNA extraction and analysis with DNA probes for determinant classes A to E resulted in no hybridization signal, because of the low number of gram-negative tetracycline-resistant bacteria. Identification of class E isolates revealed that this determinant is present not only in Aeromonas hydrophila, Escherichia coli, and Vibrio salmonicida but also in additional strains.
authors.affiliation: Department of General Microbiology, University of Copenhagen, Denmark.
authors.forename: S R
authors.initials: SR
authors.lastname: Andersen
authors.forename: R A
authors.initials: RA
authors.lastname: Sandaa
mesh_terms: D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D003718:Denmark; D006088:Gram-Negative Aerobic Bacteria / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D008506:Medical Waste; D009664:Norway; D012623:Seawater; D012722:Sewage; D013753:Tetracycline Resistance / Q000235:genetics*; D014871:Water Microbiology; D014876:Water Pollution
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D008506:Medical Waste; D012722:Sewage