The human vigilin gene: identification, chromosomal localization and expression pattern.
Chick vigilin cRNA clones were used to isolate the cognate human gene, by screening a pWE15 genomic library. Three independent cosmid clones were isolated and characterized by restriction mapping. The gene was identified by sequencing an internal EcoRI fragment containing two exons homologous to exon 24 and 25 of the chicken vigilin gene and corresponding to nucleotides 1973-2104 of the human HBP-cDNA. The homology between the chicken and human sequences was 77% and 82% at the cDNA level, and 91% and 100% at the amino acid level. In addition, the analyzed intron/exon boundaries were invariantly conserved. The 5' and 3' regions of the human gene were mapped by Southern analysis of the respective clones with synthetic oligonucleotides. The entire vigilin gene spans a region of about 50 kb and has been assigned to chromosome 2q36-q37.2 (FL-pter value of 0.96 +/- 0.03) by fluorescence in situ hybridization to metaphase spreads from normal peripheral blood lymphocytes. The vigilin gene is localized in a chromosomal region comprising a cluster of collagen genes (COLIVA3, COLVIA3) and the locus of the Waardenburg syndrome I. Only one mRNA species of 4.4 kb is transcribed from the human vigilin gene. In accordance with previous observations on chicken mRNA, the expression of the human vigilin mRNA depends on the stage of cytodifferentiation both in vitro and in situ.
Plenz
G
G
Institut für Medizinische Molekularbiologie, Medizinische Universität zu Lübeck, Germany.
Kügler
S
S
Schnittger
S
S
Rieder
H
H
Fonatsch
C
C
Müller
P K
PK
D015152:Blotting, Northern; D002352:Carrier Proteins; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D003360:Cosmids; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins; D015183:Restriction Mapping
D002352:Carrier Proteins; D011506:Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C075197:high density lipoprotein binding protein