title: Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior.
abstract: In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.
authors.affiliation: Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208.
authors.forename: M H
authors.initials: MH
authors.lastname: Vitaterna
authors.forename: D P
authors.initials: DP
authors.lastname: King
authors.forename: A M
authors.initials: AM
authors.lastname: Chang
authors.forename: J M
authors.initials: JM
authors.lastname: Kornhauser
authors.forename: P L
authors.initials: PL
authors.lastname: Lowrey
authors.forename: J D
authors.initials: JD
authors.lastname: McDonald
authors.forename: W F
authors.initials: WF
authors.lastname: Dove
authors.forename: L H
authors.initials: LH
authors.lastname: Pinto
authors.forename: F W
authors.initials: FW
authors.lastname: Turek
authors.forename: J S
authors.initials: JS
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D002940:Circadian Rhythm / Q000235:genetics*; D005038:Ethylnitrosourea; D005260:Female; D005796:Genes; D005838:Genotype; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016296:Mutagenesis; D010641:Phenotype
chemical_list: D005038:Ethylnitrosourea