Systematic study of the 3-hydroxy fatty acid composition of mycobacteria.
Twenty-seven strains belonging to 12 Mycobacterium species were studied for 3-hydroxy fatty acid composition. Mycobacterial cells were subjected to both mild and strong acid methanolysis, after which the liberated hydroxy fatty acids were purified and analyzed by gas chromatography-mass spectrometry as methyl ester trimethylsilyl ether derivatives. Altogether, 21 3-hydroxy fatty acids containing 14 to 28 carbon atoms were detected; 10 were straight chain, 6 were 2-methyl branched chain, and 5 were 2,4,6-trimethyl branched chain. The mycobacterial strains were classified in groups according to 3-hydroxy fatty acid patterns.
Alugupalli
S
S
Department of Medical Microbiology, University of Lund, Sweden.
Portaels
F
F
Larsson
L
L
D004951:Esterification; D005227:Fatty Acids / Q000032:analysis*; D005233:Fatty Alcohols / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D009161:Mycobacterium / Q000737:chemistry*; D013045:Species Specificity
D005227:Fatty Acids; D005233:Fatty Alcohols; D000432:Methanol