The murine interleukin-5 receptor alpha-subunit gene: characterization of the gene structure and chromosome mapping.
To understand better the regulation of interleukin-5 receptor alpha-subunit (IL-5R alpha) expression, we have isolated the genomic clones of mouse IL-5R alpha (mIL-5R alpha) and analyzed the structure of the gene. The gene spans more than 35 kb and is composed of 11 exons. We found that two mRNAs encoding secreted forms of mIL-5R alpha result from differential splicing events. We identified the transcriptional start site by primer extension analysis of mIL-5R alpha mRNA. Nucleotide sequence of the 5'-flanking region contains potential binding sites for transcription factor Ap1, AP-1, GATA-1, and PU.1. About 260 bp sequence of the 5'-flanking region exhibited promoter activity when it was linked to a promoterless bacterial chloramphenicol acetyltransferase (CAT) gene. The promoter activity was seen not only in the IL-5-dependent pre-B-cell line Y16, but also in fibroblast cell line NIH-3T3. Comparison of the exon-intron boundaries of mIL-5R alpha genes with those of other members of the cytokine receptor family reveals a conserved evolutionary structure. By fluorescence in situ hybridization analysis, the mIL-5R alpha gene has been assigned to chromosome 6.
Imamura
F
F
Department of Cell Differentiation, Kumamoto University School of Medicine, Japan.
Takaki
S
S
Akagi
K
K
Ando
M
M
Yamamura
K
K
Takatsu
K
K
Tominaga
A
A
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000378:metabolism; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D005091:Exons; D015698:Genomic Library; D007438:Introns; D008099:Liver / Q000378:metabolism; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics*; D053649:Receptors, Interleukin-5; D015183:Restriction Mapping; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
D046911:Macromolecular Substances; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D014157:Transcription Factors; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase