G protein Gi2 alpha in the cochlea: cloning and selective occurrence in receptor cells.
A cDNA library was made from the mouse cochlea and screened with a G protein-cDNA like molecule obtained from cochlear tissue by polymerase chain reaction. The nucleotide sequence of a clone, named cochlear Gi2 alpha, had 99.2% identity to mouse macrophage Gi2 alpha. Using an antibody which is selective for Gi2 alpha, expression of the cochlear Gi2 alpha was localized in outer and inner hair cells of the organ of Corti. Possible functional roles of this G protein in hair cells are discussed.
Tachibana
M
M
Laboratory of Molecular Biology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892.
Asano
T
T
Wilcox
E
E
Yokotani
N
N
Rivolta
M N
MN
Fex
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D019204:GTP-Binding Proteins / Q000235:genetics*; D015723:Gene Library; D006198:Hair Cells, Auditory / Q000737:chemistry*; D007150:Immunohistochemistry; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D019204:GTP-Binding Proteins