title: White blood cells in semen affect hyperactivation but not sperm membrane integrity in the head and tail regions.
abstract: The presence of high numbers of peroxidase-positive PML in ejaculated semen significantly reduced sperm HA, an important step leading to sperm capacitation. Sperm membranes at both the head and tail regions, as assessed by the hypo-osmotic viability parameter and the hypo-osmotic sperm swelling test, respectively, were not affected by peroxidase-containing leukocytes. Sperm motility was not affected, but sperm curvilinear and straight line velocity parameters were reduced in the presence of high concentrations of leukocytes in the ejaculate. The results suggested that the effect of leukocytes on sperm was through a reduction in sperm hyperactive motility but not through alterations in the sperm head and tail membranes.
authors.affiliation: Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, California 92350.
authors.forename: P J
authors.initials: PJ
authors.lastname: Chan
authors.forename: B C
authors.initials: BC
authors.lastname: Su
authors.forename: D R
authors.initials: DR
authors.lastname: Tredway
authors.forename: E A
authors.initials: EA
authors.lastname: Whitney
authors.forename: S C
authors.initials: SC
authors.lastname: Pang
authors.forename: J
authors.initials: J
authors.lastname: Corselli
authors.forename: J D
authors.initials: JD
authors.lastname: Jacobson
mesh_terms: D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007962:Leukocytes / Q000166:cytology*; D008297:Male; D009995:Osmosis; D009195:Peroxidase; D012661:Semen / Q000166:cytology*; D013077:Sperm Head / Q000502:physiology* / Q000648:ultrastructure; D013081:Sperm Motility / Q000502:physiology*; D013082:Sperm Tail / Q000502:physiology* / Q000648:ultrastructure; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure
chemical_list: D009195:Peroxidase