title: Prevention of ongoing lipid peroxidation by wound excision and superoxide dismutase treatment in the burned rat.
abstract: To determine if wound excision and superoxide dismutase (SOD) treatment prevent ongoing lipid peroxidation after burn injury, the plasma, kidney, and lung lipid peroxide (LPO) levels in 25% total body surface area (TBSA) burned rats was studied. The animals were given intraperitoneal bovine copper-, zinc-SOD (Cu/Zn-SOD) (50,000 U/kg dissolved in saline) or saline immediately after burns and were operated by wound debridement and allograft 2 hours after the burn. We measured LPO levels of 6-hour postburn plasma and tissues by the thiobarbituric acid (TBA) method, and measured the manganese SOD (Mn-SOD) by an enzyme-linked immunosorbent assay. Wound excision alone prevented the increase of plasma LPO levels but could not prevent the increase in tissues. The combination of wound excision and Cu/Zn-SOD treatment markedly inhibited the increase in both plasma and tissue LPO levels after the burn, but did not prevent the increase in Mn-SOD. Wound excision in conjunction with SOD-treatment might be therapeutic in the management of severe burns.
authors.affiliation: Department of Traumatology and Emergency Medicine, National Defense Medical College, Tokorozawa, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Saitoh
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Ookawara
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Takahara
authors.forename: S
authors.initials: S
authors.lastname: Ishihara
authors.forename: H
authors.initials: H
authors.lastname: Ohno
authors.forename: K
authors.initials: K
authors.lastname: Mimura
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000628:therapy*; D003131:Combined Modality Therapy; D003646:Debridement / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000032:analysis*; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008297:Male; D015999:Multivariate Analysis; D051381:Rats; D017208:Rats, Wistar; D016038:Skin Transplantation / Q000379:methods*; D013482:Superoxide Dismutase / Q000032:analysis / Q000627:therapeutic use*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors
chemical_list: D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase