[Why do endothelial cells require adenosine triphosphate?].
NMR-spectroscopy (31P-NMR, 23Na-NMR) has been used to characterize the energy metabolism of cultured endothelial cells of the pig aorta and to determine the energy requirements of the Na-K-ATPase relative to total cellular energy consumption. Endothelial cells exhibited high concentrations of creatine phosphate and cardiac microvascular creatine kinase (CK) was of the BB-CK subtype. BB-CK can therefore be used as a marker-enzyme of endothelial cells in muscular organs. The transmembrane flux-rate of sodium was 117 mumol Nalmin/mg prot in the steady state at an intracellular sodium concentration of 25 mmol/l. Combining microcalorimetric measurements with 23Na-NMR data revealed that the energy requirement of endothelial Na-K-ATPase despite the high surface to volume ratio comprises only 3-5% of total cellular energy consumption.
Schrader
J
J
Institut für Herz- und Kreislaufphysiologie der Heinrich-Heine-Universität Düsseldorf.
Gruwel
M
M
Decking
U
U
Alves
C
C
D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology; D006801:Humans; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013552:Swine
D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase
Wozu benötigen Endothelzellen Adenosintriphosphat?