Identification of novel cDNAs encoding human kexin-like protease, PACE4 isoforms.
PACE4 has been identified as a second human subtilisin-like protease by Keifer et al. [DNA and Cell Biology (1991) 10, 757-769]. In this study, we isolated two novel cDNAs coding for PACE4 isoforms (PACE4C and PACE4D) from a human placenta cDNA library. The deduced PACE4C protein sequence (652 amino acids) lacks the cysteine-rich region located at the carboxy terminus of PACE4. The DNA sequence of the 3'-untranslated region (1 kb) of PACE4C is not homologous to the PACE4 sequence. The protein (497 amino acids) encoded by PACE4D cDNA lacks a signal peptide, a propeptide and the cysteine-rich region. These isoform cDNAs were also isolated from rat pituitary cDNA library.
Tsuji
A
A
Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, Japan.
Higashine
K
K
Hine
C
C
Mori
K
K
Tamai
Y
Y
Nagamune
H
H
Matsuda
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D015723:Gene Library; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D010903:Pituitary Gland, Anterior / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D043484:Proprotein Convertases; D051381:Rats; D012697:Serine Endopeptidases / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D007527:Isoenzymes; C080654:PCSK6 protein, human; C513087:Pcsk6 protein, rat; D043484:Proprotein Convertases; D012697:Serine Endopeptidases