Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior.
In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.
Vitaterna
M H
MH
Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208.
King
D P
DP
Chang
A M
AM
Kornhauser
J M
JM
Lowrey
P L
PL
McDonald
J D
JD
Dove
W F
WF
Pinto
L H
LH
Turek
F W
FW
Takahashi
J S
JS
D000818:Animals; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D002940:Circadian Rhythm / Q000235:genetics*; D005038:Ethylnitrosourea; D005260:Female; D005796:Genes; D005838:Genotype; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016296:Mutagenesis; D010641:Phenotype
D005038:Ethylnitrosourea