Ras transformation alters the composition of extracellular matrix proteoglycans in rat fibroblasts.
ts371 KiMuSV-transformed rat kidney fibroblasts growing at the permissive temperature to allow the expression of a transforming p21 ras protein secrete a different pattern of chondroitin/dermatan sulfate proteoglycans than when growing at the non-permissive temperature. Transformed cells produce less versican and larger amounts of biglycan and decorin than their normal counterpart, and they do not secrete 35S-labeled PG-100. Western blot experiments showed the presence of PG-100 epitope, suggesting that the apparent effect of the oncoprotein is due to a reduced sulfation of the molecule. Transformed and non-transformed cells are similarly sensitive to the proteoglycan-induction effect of TGF-beta.
Romarís
M
M
Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Villena
J
J
Molist
A
A
Heredia
A
A
Bassols
A
A
D000818:Animals; D058578:Biglycan; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D011905:Genes, ras; D037181:Lectins, C-Type; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053675:Versicans
C546886:Bgn protein, rat; D058578:Biglycan; D011508:Chondroitin Sulfate Proteoglycans; C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; C508324:Vcan protein, rat; D053675:Versicans