Lectin histochemistry on the skin of hairless descendants of Mexican hairless dogs.
The skin of hairless descendants of Mexican hairless dogs was investigated lectin-histochemically. Haired dogs were also used for comparative study. In the epidermis of infant pups, lectin staining revealed marked differences between hairless and haired dogs. Griffonia simplicifolia-I (GS-I) bound moderately to the cytoplasm of the stratum basale of hairless dogs, while no binding to that of haired dogs was observed. Bauhinia purpurea (BPA) showed positive staining for the stratum spinosum and basale of hairless dogs, while it was negative or weakly positive for the cytoplasm of both epidermal layers of haired dogs. Maclura pomifera (MPA) showed moderate to intense staining for the intercellular area of the stratum basale of hairless dogs, while no or weak staining was detected in that of haired dogs. Urex europeus-I (UEA-I) bound weakly to moderately to the cytoplasm and the intercellular area of the stratum spinosum of hairless dogs, whereas no or very weak staining was observed in those of haired dogs. Most of the lectins exhibited significant alterations in the epidermal staining pattern between infants and adults. Significant differences were not observed in adult canine epidermis between hairless and haired dogs. In conclusion, lectin histochemistry in hairless dogs revealed that there were different development steps in the epidermal cells between infants and adults.
Miyazawa
M
M
Research Center, Nihon Nosan Kogyo Co. Ltd., Chiba, Japan.
Kimura
T
T
Itagaki
S
S
D000818:Animals; D004285:Dogs / Q000235:genetics / Q000378:metabolism*; D004817:Epidermis / Q000737:chemistry*; D005260:Female; D005799:Genes, Dominant; D006023:Glycoproteins / Q000032:analysis*; D006651:Histocytochemistry; D037102:Lectins / Q000032:analysis*; D008297:Male; D008800:Mexico; D010641:Phenotype
D006023:Glycoproteins; D037102:Lectins