Molecular and biological characterization of fusion regulatory proteins (FRPs): anti-FRP mAbs induced HIV-mediated cell fusion via an integrin system.
Anti-FRP mAbs induced polykaryocyte formation of U2ME-7 cells (CD4+U937 cells transfected with the HIV gp160 gene). Anti-FRP-1 mAb immunoprecipitated gp80-85, gp120 and homodimers of these peptides, and anti-FRP-2 mAb reacted with gp135 identically to the alpha 3 subunit of integrin. Both anti-FRP-1 and anti-FRP-2 mAb-induced cell fusion was blocked by anti-beta 1 integrin antibody, fibronectin or inhibiting anti-FRP-1 antibody. Therefore, anti-FRP mAbs were thought to induce the fusion via an integrin system(s). FRP-mediated fusion was temperature, cytoskeleton, energy and Ca2+ dependent. These experiments showed a possible regulatory function of cell fusion by an integrin system(s).
Ohta
H
H
Department of Microbiology, Mie University School of Medicine, Japan.
Tsurudome
M
M
Matsumura
H
H
Koga
Y
Y
Morikawa
S
S
Kawano
M
M
Kusugawa
S
S
Komada
H
H
Nishio
M
M
Ito
Y
Y
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D001483:Base Sequence; D002459:Cell Fusion; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D017931:DNA Primers; D005353:Fibronectins / Q000378:metabolism; D015686:Gene Products, env / Q000276:immunology / Q000378:metabolism; D015726:Giant Cells / Q000382:microbiology; D015699:HIV Envelope Protein gp120 / Q000276:immunology / Q000378:metabolism; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000276:immunology / Q000378:metabolism; D016029:Receptors, Very Late Antigen / Q000378:metabolism; D014162:Transfection
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D005353:Fibronectins; D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D016023:Integrins; D007797:Laminin; D011498:Protein Precursors; D016029:Receptors, Very Late Antigen; D003094:Collagen