Chemical characterization of sialyl oligosaccharides isolated from tammar wallaby (Macropus eugenii) milk.
Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)1(Gal)9(GlcNAc)2(Neu5Ac)1, and (Glc)1(Gal)8(GlcNAc)2(Neu5Ac)2. Their chemical structures were further elucidated by 1H-NMR and methylation analysis. The results suggest that their approximate structures are: [formula: see text]
Urashima
T
T
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Saito
T
T
Tsuji
Y
Y
Taneda
Y
Y
Takasawa
T
T
Messer
M
M
D000818:Animals; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007614:Macropodidae / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification
D009844:Oligosaccharides