A possible osmodependent protease in Escherichia coli.
When a strain of Escherichia coli, expressing a hybrid protein GalK-beta-Gal, is shifted to high osmolarity, the beta-galactosidase activity strongly decreases within 20 min of shock. The loss of beta-galactosidase activity results from degradation of the hybrid protein under osmotic stress. The results raise the possibility that osmotic stress induces a specific osmodependent protease.
Meury
J
J
Institut Jacques Monod, Paris, France.
D010450:Endopeptidases / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005686:Galactokinase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D009995:Osmosis / Q000502:physiology*; D009997:Osmotic Pressure; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D005686:Galactokinase; D001616:beta-Galactosidase; D010450:Endopeptidases