title: Kappa immunoglobulin promoters and enhancers display developmentally controlled interactions.
abstract: We have investigated the interaction of the kappa immunoglobulin light chain intron and 3' enhancers with two different kappa promoters at distinct stages of B-cell development. We find that transiently transfected reporter gene constructs driven by either the kappa V-region promoter, or the kappa germline promoter, are controlled by the known enhancers of the locus in a developmentally regulated fashion. We have, however, observed differences in promoter activation by each enhancer. Moreover, constructs controlled by a combination of both enhancers are synergistically activated at the B-cell and plasma cell stages as compared with constructs containing either enhancer alone. This synergy is not observed early in development, at the pre-B cell stage. The pattern of enhancer and promoter interactions is discussed in the context of the known developmental regulation of the locus.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.
authors.forename: R
authors.initials: R
authors.lastname: Fulton
authors.forename: B
authors.initials: B
authors.lastname: Van Ness
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007438:Introns; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D007145:Immunoglobulin kappa-Chains; D008070:Lipopolysaccharides