Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts.
The co-cultures of five different human tumor cell lines with human normal fibroblasts significantly stimulated the production of tissue inhibitors of metalloproteinases-1 (TIMP-1) when compared to cultures of individual cells. In the co-culture of T24 human urinary bladder carcinoma cells and CCD18 human fibroblasts, production of both TIMP-1 and metalloproteinases was stimulated, and the stimulatory effects were dependent on the cellular ratio between the fibroblasts and carcinoma cells. On day 6 of culture, collagenase and stromelysin were stimulated at a ratio of CCD18 fibroblasts to T24 cells of 1:0.1, while the maximum TIMP-1 production occurred at a ratio of 1:1. Thus, the cellular ratio in the interaction of carcinoma cells with host fibroblasts affects the production of TIMP-1 and metalloproteinases and hence modulates the balance between them.
Nabeshima
K
K
Department of Pathology, Miyazaki Medical College, Japan.
Kishi
J
J
Kurogi
T
T
Komada
N
N
Kataoka
H
H
Okada
Y
Y
Koono
M
M
D002478:Cells, Cultured; D003470:Culture Media; D004790:Enzyme Induction; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism*; D019714:Tissue Inhibitor of Metalloproteinases; D014407:Tumor Cells, Cultured / Q000201:enzymology*
D003470:Culture Media; D006023:Glycoproteins; D019714:Tissue Inhibitor of Metalloproteinases; D008666:Metalloendopeptidases