Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells.
MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer cell line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance was due to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.
Futscher
B W
BW
Bone Marrow Transplant Program, Arizona Cancer Center, Tucson 85724.
Abbaszadegan
M R
MR
Domann
F
F
Dalton
W S
WS
D001483:Base Sequence; D004351:Drug Resistance / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008942:Mitoxantrone / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D008942:Mitoxantrone