Sequence of the spike protein of the porcine epidemic diarrhoea virus.
The complete sequence of the spike (S) gene of the Br1/87 isolate of porcine epidemic diarrhoea virus (PEDV) was determined from cDNA clones. The predicted polypeptide was 1383 amino acids long, contained 29 potential N-linked glycosylation sites and showed structural features similar to those of the coronavirus spike protein. The PEDV S protein, like that of the members of the transmissible gastroenteritis virus (TGEV)-related subset, lacks a proteolytic site to yield cleaved amino and carboxy subunits S1 and S2. Viral polypeptide species of the expected M(r), i.e. 170K/190K, were observed in PEDV-infected cells. Sequence comparison confirmed that, within the subset, PEDV was most closely related to the human respiratory coronavirus HCV 229E. However, PEDV S protein has an additional 250 residue N-terminal domain which is absent from HCV 229E and porcine respiratory coronavirus, the respiratory variant of TGEV. Alignment of the S1 regions revealed a second domain of about 90 residues with increased sequence divergence which might possibly express virus-specific determinants.
Duarte
M
M
I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, France.
Laude
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003332:Coronaviridae / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D064370:Spike Glycoprotein, Coronavirus; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000145:classification / Q000235:genetics*
D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus