title: Primed in situ DNA amplification (PIDA).
abstract: A novel PCR in situ protocol is described which can be implemented in one day. An initial amplification step with a single primer pair is followed by an amplification with an internal oligonucleotide probe with labeled nucleotides (dig-dUTP). Detection is then accomplished with an antidigoxigenin antibody and substrate solution. This rapid and sensitive method may be useful in diagnosing low copy DNA in tissue specimens and cells.
authors.affiliation: Allergic Diseases Section, NIAID, National Institute of Health, Bethesda, Maryland.
authors.forename: R B
authors.initials: RB
authors.lastname: Moss
authors.forename: M A
authors.initials: MA
authors.lastname: Kaliner
mesh_terms: D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005784:Gene Amplification; D006678:HIV / Q000235:genetics* / Q000302:isolation & purification*; D006679:HIV Seropositivity / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods* / Q000706:statistics & numerical data; D008198:Lymph Nodes / Q000382:microbiology; D015336:Molecular Probe Techniques; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral