Antigen presentation by macrophages from bacille Calmette-Guérin (BCG)-resistant and -susceptible mice.
We have compared the antigen-presenting capacity of macrophages from congenic BALB/c.Bcgr and BALB/c.Bcgs mice that differentially express MHC class II glycoproteins. Several different criteria were used to evaluate the presentation of a protein antigen, ovalbumin (OVA), including limiting the concentration of antigen or the numbers of macrophages, and using both native OVA and OVA peptide 323-339. No differences in the capacity of macrophages from Bcgr and Bcgs mice to present antigen to a OVA-specific T cell hybridoma were found. Splenic macrophages from BCG-infected congenic mice also induced an equivalent amount of IL-2 production by the T cell hybridoma. The relationship of these findings to other differences that have been attributed to Bcg are discussed.
Hilburger
M E
ME
Department of Microbiology, College of Biological Sciences, Ohio State University, Columbus 43210.
Zwilling
B S
BS
D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects; D000942:Antigens, Bacterial / Q000276:immunology*; D002738:Chloroquine / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D009163:Mycobacterium bovis / Q000276:immunology* / Q000472:pathogenicity; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D013154:Spleen / Q000166:cytology
D000942:Antigens, Bacterial; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; D007371:Interferon-gamma; D002738:Chloroquine; D010047:Ovalbumin