Application of a flow injection fibre optic biosensor for the analysis of different amino acids.
The analysis of different amino acids is described using a fibre optic biosensor previously described in its principles. This biosensor works according to the measurement principle of flow injection analysis (FIA) and consists of a fluorosensor and a measurement cell operating under pressure. In the measurement cell, enzymes and molecular weight enlarged coenzyme PEG (MW 20000)-N6-(2-aminoethyl)-NAD(H) are confined behind a solid ultrafiltration membrane. Assays for the analysis of L-phenylalanine and L-alanine were developed. The analysis frequency is in the range of 1 to 2 samples per hour, and the sensor stability was found to be sensitive to the stability of each enzyme system used. This effect was studied in detail. The L-alanine assay was found to be especially reliable, sensitive, specific, and highly selective for the L-enantiomer.
Lee
S J
SJ
Institut für Technische Chemie, Universität Hannover, Germany.
Scheper
T
T
Bückmann
A F
AF
D000409:Alanine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis*; D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D017022:Flow Injection Analysis; D009862:Online Systems; D010649:Phenylalanine / Q000032:analysis; D013237:Stereoisomerism
D000596:Amino Acids; D010649:Phenylalanine; D000409:Alanine