title: Cloning, structure, cellular localization, and possible function of the tumor suppressor gene lethal(3)malignant blood neoplasm-1 of Drosophila melanogaster.
abstract: The tumor suppressor gene, lethal(3)malignant blood neoplasm-1+, of Drosophila melanogaster is required for the differentiation of the phagocytic blood-cell type, the plasmatocyte. In the homozygously mutated state it causes the malignant transformation of these blood cells. We present here the cloning, sequencing, structure, and expression of the l(3)mbn-1+ gene during development. The cloned gene was identified by germ-line transformation, generation of revertants, and the detection of the corresponding mRNA in blood cells and other tissues. Homologies of the G-S-rich C-terminus of the putative MBN83 protein to human cytokeratins K1, K10, and mouse loricrin were found. The structure and possible function of the wild-type l(3)mbn-1+ gene are discussed.
authors.affiliation: Institut für Genetik, Johannes Gutenberg Universität, Mainz, Federal Republic of Germany.
authors.forename: L
authors.initials: L
authors.lastname: Konrad
authors.forename: G
authors.initials: G
authors.lastname: Becker
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.forename: T
authors.initials: T
authors.lastname: Klöckner
authors.forename: G
authors.initials: G
authors.lastname: Kaufer-Stillger
authors.forename: S
authors.initials: S
authors.lastname: Dreschers
authors.forename: J E
authors.initials: JE
authors.lastname: Edström
authors.forename: E
authors.initials: E
authors.lastname: Gateff
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001773:Blood Cells / Q000473:pathology*; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004331:Drosophila melanogaster / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger