Epidermal growth factor induces biphasic activation of ornithine decarboxylase in human stomach-derived KATO-III cells.
Effect of epidermal growth factor (EGF) on ornithine decarboxylase (ODC) was examined in human gastric cancer-derived KATO-III cells, because 125I-EGF binding studies indicated a presence of specific binding sites for EGF on these cells. Upon stimulation with EGF, both ODC mRNA expression and ODC enzyme activity were significantly increased in KATO-III cells. However, unlike in other cellular systems, both EGF-induced ODC mRNA expression and ODC enzyme activation were biphasic with the peaks at 15 +/- 10 min and 2.1 +/- 1.5 hrs (mean +/- SE) for mRNA, and 3.1 +/- 1.5 and 7.7 +/- 1.8 hrs (mean +/- SE) for enzyme activity, respectively. Therefore, KATO-III cell line may provide a unique model for the biochemical analysis of EGF action on ODC activation.
Ishikawa
T
T
DVA Medical Center, Long Beach.
Mitsuhashi
M
M
Ichikawa
Y
Y
Tarnawski
A
A
D002460:Cell Line; D017931:DNA Primers; D004790:Enzyme Induction; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D013270:Stomach; D013274:Stomach Neoplasms; D013997:Time Factors; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D009955:Ornithine Decarboxylase