title: [Structure-activity organization of the variola virus genome. III. Sequencing and analysis of the nucleotide sequence of the conserved region of HindIII-F, -N-, and -A-fragments of the India 1967 strain genome].
abstract: Computer analysis of variola major virus (VAR) genomic fragment bounded by open reading frames (ORFs) D1R and A33L which is 47,961 bp long revealed 46 potential ORFs. The VAR proteins were compared with the analogous proteins of vaccinia virus strain Copenhagen. The subunits of DNA-dependent RNA polymerase, as well as the transcription factors, mRNA capping enzymes, and proteins necessary for the virion morphogenesis proved to be highly conservative within orthopoxviruses. The most pronounced differences between the VAR genome fragment under study and the corresponding vaccinia virus fragment were revealed in the vicinity of the gene encoding the A-type inclusion body protein. The possible functions of the analyzed viral proteins are discussed.
authors.forename: S N
authors.initials: SN
authors.lastname: Shchelkunov
authors.forename: S M
authors.initials: SM
authors.lastname: Resenchuk
authors.forename: A V
authors.initials: AV
authors.lastname: Totmenin
authors.forename: A A
authors.initials: AA
authors.lastname: Kolykhalov
authors.forename: I V
authors.initials: IV
authors.lastname: Frolov
authors.forename: S M
authors.initials: SM
authors.lastname: Dryga
authors.forename: V V
authors.initials: VV
authors.lastname: Volchkov
authors.forename: V E
authors.initials: VE
authors.lastname: Chizhikov
authors.forename: V V
authors.initials: VV
authors.lastname: Gutorov
authors.forename: V M
authors.initials: VM
authors.lastname: Blinov
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015247:Deoxyribonuclease HindIII; D016679:Genome, Viral; D007181:Inclusion Bodies, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014157:Transcription Factors / Q000378:metabolism; D012901:Variola virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D014771:Virion
chemical_list: D012367:RNA, Viral; D014157:Transcription Factors; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases; D015247:Deoxyribonuclease HindIII
vernacular_title: Izuchenie strukturno-funktsional'noĭ organizatsii genoma virusa natural'noĭ ospy. III. Sekvenirovanie i analiz posledovatel'nosti nukleotidov konservativnogo raĭona HindIII-F-, -N-, i -A-fragmentov genoma shtamma Indiia-1967.