A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes.
We have constructed a restriction map of the human genomic region containing the genes encoding the three members of the interleukin-1 (IL-1) family, IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist (IL-1 ra). For this purpose, pulsed-field gel electrophoresis blots were hybridized with probes derived from the three genes. The genes (IL1A, IL1B, and IL1RN, respectively) were found to map to a common restriction fragment of approximately 430 kb that is flanked by two clusters of sites for methylation-sensitive rare-cutter restriction enzymes (putative CpG islands). A likely third internal CpG island was marked by two rare-cutter sites. CpG and non-CpG-specific enzymes were used to map the three genes. Relative to one terminal CpG island, the three genes were mapped to the following intervals: IL1A was between +0 and +35 kb, IL1B was between +70 and +110 kb, and IL1RN was between +330 and +430 kb.
Nicklin
M J
MJ
Department of Medicine and Pharmacology, University of Sheffield, Royal Hallamshire Hospital, United Kingdom.
Weith
A
A
Duff
G W
GW
D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D004262:DNA Restriction Enzymes; D016521:Electrophoresis, Gel, Pulsed-Field; D005796:Genes; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D017472:Receptors, Interleukin-1 / Q000235:genetics*
D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D004262:DNA Restriction Enzymes