Preparation and properties of metabolically 3H- or 13C-labeled (1-->3)-beta-D-glucan, SSG, from Sclerotinia sclerotiorum IFO 9395.
Metabolically labeled (1-->3)-beta-D-glucan (SSG) obtained from the culture filtrate of Sclerotinia sclerotiorum IFO 9395, with the radio (3H) or the stable isotope (13C) was prepared. The specific radioactivity of 3H-SSG was increased in accordance with the amount of D-[3H]glucose added, and was 15-20 kBq/mg SSG when 222 kBq/mL of D-[3H]glucose was added. Physicochemical analyses of 3H-SSG suggested a structural similarity between SSG and 3H-SSG. 13C NMR spectra of 13C-labeled SSG (13C-SSG) revealed that a strong signal of the 13C-nucleus was observed at the C-1 or C-2 position when D-[1-13C]glucose or D-[2-13C]glucose was used, respectively, suggesting that most of the glucose residues in SSG were directly taken up from the medium. In addition, part of the 13C-nucleus was incorporated into the SSG molecule at all carbon atoms after metabolic degradation and reconstruction of the glucose molecule. Analyses of the culture filtrate and the mycelium of the fungus suggested that part of the glucose was also metabolized to trehalose and mannitol.
Suda
M
M
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
Ohno
N
N
Adachi
Y
Y
Yadomae
T
T
D001203:Ascomycota / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002247:Carbon Isotopes; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D005947:Glucose / Q000378:metabolism; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D014316:Tritium; D047071:beta-Glucans
D002247:Carbon Isotopes; D005936:Glucans; D047071:beta-Glucans; D014316:Tritium; C033363:beta-1,3-glucan; D005947:Glucose