Production, partial purification and characterisation of amyloglucosidase from a new variety of Aspergillus candidus Link var. aureus.
The production of amyloglucosidase by a new variety of Aspergillus candidus was studied on various natural carbon and nitrogen nutrient sources. Jowar starch and peanut meal were found to be the best nutrient sources of carbon and nitrogen respectively. The maximum enzyme productivity was observed at pH 6.5 and temperature 32 degrees C. Sodium fluoride was found to inhibit enzyme amyloglucosidase to an extent of 85%. Addition of trace metals viz. Zinc, copper and potassium in the medium impaired the production of the enzyme. A four fold purification of the enzyme was achieved with ammonium sulphate precipitation. The partially purified enzyme gave 84% conversion efficiency of starch to glucose in 10% starch solution without the addition of liquefying enzyme.
Narayanan
M S
MS
Industrial Microbiology Division, Research and Development, Hindustan Antibiotics Ltd., Pimpri, Pune, India.
Ambedkar
S S
SS
D001230:Aspergillus / Q000201:enzymology*; D003470:Culture Media; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013213:Starch / Q000378:metabolism; D013696:Temperature; D014131:Trace Elements
D003470:Culture Media; D014131:Trace Elements; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose