title: Influence of urological irrigation fluids on urothelial bacterial adherence.
abstract: The influence of various urological irrigation solutions on bacterial growth and adherence to urothelium was investigated in in vitro and guinea pig models. The irrigation solutions glycine 1.5%, glycine 1.5% and ethanol 1%, glycerol 3%, mannose 6%, sorbitol 2.7% and mannitol 0.54% all inhibited bacterial growth compared with normal saline. In guinea pigs, the influence on bacterial adherence of four irrigation solutions (glycine 1.5%, glycine 1.5% and ethanol 1%, mannose 6%, povidone-iodine) was investigated using two different strains of E. coli. After cauterizing one side of the bladder and inoculation with 2.7 x 10(8) colony forming units under high or low pressure, the bladder was irrigated with the irrigation solutions. There was a stronger adherence of E. coli O6 (with type I pili) than of E. coli ATCC 25922 (without type I pili) to bladder urothelium, particularly to the injured side. There was no significant difference between the high- and low-pressure groups. None of the various irrigation solutions was clearly superior. As mannose 6% effectively inhibited type I pili and also had some antibacterial activity it may reduce urinary tract infection if used as irrigation solution.
authors.affiliation: Urology Clinics, University Hospital, Basel, Switzerland.
authors.forename: T C
authors.initials: TC
authors.lastname: Gasser
authors.forename: P O
authors.initials: PO
authors.lastname: Madsen
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000502:physiology; D006168:Guinea Pigs; D012996:Solutions; D007507:Therapeutic Irrigation; D001743:Urinary Bladder / Q000382:microbiology*
chemical_list: D012996:Solutions