Comparative analysis of the protein components from 5S rRNA.protein complexes of halophilic archaebacteria.
The 5S RNA.protein complexes have been isolated from the 50S subunit of the halophilic archaebacteria Halobacterium cutirubrum, Halobacterium halobium, Halobacterium salinarium, Haloferax mediterranei, Haloferax volcanii and Haloarcula marismortui. The 50S subunits from most of the halophiles released a multiprotein ribonucleoprotein particle similar to that previously observed with the H. cutirubrum 5S RNA.protein complex, which contained proteins from the L5 and L18 ribosomal protein families. Ribosomes from H. marismortui, however, released an RNA.protein complex containing a single protein (L18) that is homologous to the single protein found in the eukaryotic 5S ribonucleoprotein complexes. N-terminal sequence analyses of the halophilic 5S RNA-binding proteins suggest that the L18 protein primary structure is highly conserved, with only the H. marismortui protein having a sequence difference in at least the first twenty amino acids. Although the L5 group of ribosomal proteins also shows a high conservation, it appears that the proteins may have had more freedom to diverge throughout evolution.
McDougall
J
J
Max Planck Institut für Molekulare Genetik, Berlin, Germany.
Wittmann-Liebold
B
B
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006216:Halobacteriaceae / Q000737:chemistry*; D008969:Molecular Sequence Data; D012341:RNA, Ribosomal, 5S / Q000737:chemistry* / Q000302:isolation & purification; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D009626:Terminology as Topic
D001426:Bacterial Proteins; D012341:RNA, Ribosomal, 5S; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; C029609:ribosomal protein L18; C029742:ribosomal protein L5