Biosynthesis of Tcr-alpha, beta and Tcr-gamma, delta/CD3 complexes.
Jurkat J76 clone, LYON L12.37 clone and L12.37 cells transfected with J76-alpha cDNA or J76 Tcr-alpha mutated cDNA (J79) were analysed for membrane expression of Tcr/CD3 complex using WT31 mAb (Tcr-alpha, beta) or Tcr-delta 1 mAb (Tcr-gamma, delta): LYON cells express V beta 9 bearing Tcr-beta chains. J76 Tcr-alpha cDNA transfected LYON cells have intracellular Tcr-gamma, delta chains and J79 Tcr-alpha cDNA transfected LYON cells have intracellular Tcr-alpha (M), beta chains.
Bauguil-Caspar
S
S
Laboratoire d'Immunologie Cellulaire et Moleculaire, CRPG/CNRS, Chu de Purpan, Toulouse.
Arnaud
J
J
Kuhlmann
J
J
Geisler
C
C
Rubin
B
B
D000595:Amino Acid Sequence; D000818:Animals; D017252:CD3 Complex / Q000096:biosynthesis* / Q000235:genetics; D002999:Clone Cells / Q000276:immunology; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis* / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D017252:CD3 Complex; D018076:DNA, Complementary; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta