Autografting of feeder-cell free cultured gingival epithelium. Method and clinical application.
In oral and maxillofacial surgery an adequate mucosa/gingiva transplant is often not available in the amount required. Use of cultured gingival keratinocytes is a possibility in overcoming this problem. Keratinocytes were cultured in primary culture from gingival biopsies without xenogenous feeder-cells. The cells grew out of explants and formed, within 4 to 6 weeks, a sheet of keratinocytes up to size of 75 cm2. We have a success rate of 65% in cultures of people older than 40 years. This and the culture without feeder-cells encouraged us to use the cultured gingival cells as an autologous transplant in pre-prosthetic surgery for anterior vestibuloplasty.
Lauer
G
G
Department of Oral and Maxillo-Facial Surgery, University of Freiburg, Germany.
D000293:Adolescent; D000328:Adult; D000368:Aged; D017690:Cell Transplantation; D002478:Cells, Cultured; D004847:Epithelial Cells; D005260:Female; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes; D008297:Male; D008875:Middle Aged; D014182:Transplantation, Autologous; D014727:Vestibuloplasty / Q000379:methods*