title: FGF-2 induces regeneration of the chick limb bud.
abstract: The chick limb bud is unable to mount a regenerative response following amputation at any stage of its development. Local application of heparan sulfate conjugated beads loaded with fibroblast growth factor-2 (FGF-2) onto an amputated stage 25 chick limb bud results in a high frequency (88%) of limbs in which the regeneration of digit-like structures was induced. The regenerative response is associated with the location of the FGF-2-loaded beads on the limb stump. This is the first report of the induction of a regenerative response in a higher vertebrate limb by a chemically defined agent.
authors.affiliation: Neuroscience Interdisciplinary Training Program, Tulane University Medical School, New Orleans, Louisiana 70115.
authors.forename: G P
authors.initials: GP
authors.lastname: Taylor
authors.forename: R
authors.initials: R
authors.lastname: Anderson
authors.forename: A D
authors.initials: AD
authors.lastname: Reginelli
authors.forename: K
authors.initials: K
authors.lastname: Muneoka
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002642:Chick Embryo; D005121:Extremities / Q000196:embryology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*
chemical_list: D016222:Fibroblast Growth Factor 2