[Evaluation of the usefulness of a new generation of tests for detection in humans of antibodies for 9, 12 antigens of Salmonella. II. Comparison of agreement of DOT-ELISA, latex and passive hemagglutination tests].
The study was aimed at comparison of concordance of results obtained by Dot-ELISA, latex (OL) and passive hemagglutination (OHB) tests used for determination of antibodies for 9, 12 Salmonella antigen in serum of selected groups of humans. The basis for this comparison was frequency of detection in each of the test of antibodies for 9, 12 antigen in the titer accepted as diagnostically significant. Results of OHB and OL were concordant in 91.7%, whereas by application of OHB and Dot-ELISA it concerned 60.04% of cases. The concordance of OL and Dot-ELISA was equal to 60%. Agreement of results obtained by comparative tests was statistically significant. Parallel detection by Dot-ELISA, OL and OHB of antibodies for 9, 12 antigen in samples from patients with infection has demonstrated that complete concordance of results of all three tests was obtained in 29 out of 68 tested samples. These results suggest suitability of both Dot-ELISA and OL tests in evaluation of antibody level for antigen 9, 12 of group D Salmonella. It was found that most useful diagnostic procedure should involve parallel application of two tests: OL and Dot-ELISA or OHB and Dot-ELISA.
Szych
J
J
Zakład Bakteriologii PZH w Warszawie.
D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D007841:Latex Fixation Tests; D008875:Middle Aged; D015203:Reproducibility of Results; D012475:Salmonella / Q000276:immunology*; D012480:Salmonella Infections / Q000175:diagnosis*; D012703:Serotyping
D000907:Antibodies, Bacterial
Ocena przydatności testów nowej generacji do wykrywania u ludzi przeciwciał dla antygenu 9, 12 pałeczek Salmonella. II. Porównanie zgodności wyników odczynów: Dot-ELISA, lateksowego i hemaglutynacji biernej.