Characterization of antisera raised against Treponema denticola (ATCC 33521) whole cell, outer sheath, protoplasmic cylinder, and axial flagella.
In this study we produced polyclonal antisera directed to whole cell, outer sheath, protoplasmic cylinder and axial flagella sonicates of Treponema denticola (ATCC 33521) reference strain. Furthermore, the reactivity of the antisera was determined, using the enzyme-linked immunosorbent assay and immunoblotting techniques. As control antigen, other gram-negative bacteria (Salmonella minnesota, Escherichia coli) and related pathogenic spirochetes (Borrelia burgdorferi and Treponema pallidum) were used. It could be shown that the purified antibodies were specific for Treponema denticola and did not cross-react with the control antigens tested. Interestingly, with one exception, the anti-axial flagella antibody reacted with the flagellin of Treponema pallidum but not with Borrelia burgdorferi flagella. It is intended to use these antisera for the characterization of patient isolates in further studies.
Wolf
V
V
Robert Koch-Institut des BGA, Berlin, Germany.
Lange
R
R
D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005407:Flagella / Q000276:immunology; D007106:Immune Sera; D015151:Immunoblotting; D013205:Staphylococcal Protein A; D013347:Subcellular Fractions / Q000276:immunology; D014208:Treponema / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D013205:Staphylococcal Protein A