Myricetin and quercetin, the flavonoid constituents of Ginkgo biloba extract, greatly reduce oxidative metabolism in both resting and Ca(2+)-loaded brain neurons.
The antioxidant action of myricetin and quercetin, the flavonoid constituents of the extract of Ginkgo biloba (EGb), on oxidative metabolism of brain neurons dissociated from the rats was examined using 2',7'-dichlorofluorescin (DCFH) which is retained within the neuron and then is oxidized by cellular hydrogen peroxide to be highly fluorescent. Incubation with myricetin or quercetin reduced the oxidation of DCFH in resting brain neurons, more profoundly than EGb. Myricetin decreased the oxidative metabolism at concentrations of 3 nM or more. It was 10 nM or more for the case of quercetin. Incubation with each flavonoid constituent also reduced the Ca(2+)-induced increase in the oxidative metabolism without affecting the cellular content of DCFH or the intracellular concentrations of Ca2+. Such an antioxidant action of myricetin or quercetin may be responsible for a part of the beneficial effects of EGb on brain neurons subject to ischemia.
Oyama
Y
Y
Laboratory of Cell Signalling Pharmacology, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Fuchs
P A
PA
Katayama
N
N
Noda
K
K
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D005452:Fluoresceins; D044949:Kaempferols; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012431:Rutin / Q000494:pharmacology
D005419:Flavonoids; D005452:Fluoresceins; D044949:Kaempferols; D010936:Plant Extracts; C029569:diacetyldichlorofluorescein; D012431:Rutin; C006552:kaempferol; C040015:myricetin; D011794:Quercetin; D002118:Calcium