title: Prostacyclin production by different human grafts employed in coronary operations.
abstract: Segments of human saphenous vein, internal mammary artery, right gastroepiploic artery, and inferior epigastric artery were incubated in vitro in Krebs-Henseleit solution and compared in terms of their capacity to generate and release into the medium 6-keto-prostaglandin F1 alpha (PGF1 alpha), the stable metabolite of prostacyclin. The four vascular conduits were also challenged with endothelin-1 (40 ng/mL), and accumulation of the lipidic material in the bathing fluid was also studied. The results obtained show clearly that under both normal and endothelin-1-stimulated conditions, the four vascular segments generate a substantial amount of 6-keto-PGF1 alpha. Multiple-comparisons analysis of the results indicates that the rank order in producing 6-keto-PGF1 alpha is as follows: inferior epigastric artery > internal mammary artery > right gastroepiploic artery > saphenous vein (p < 0.01). A similar order of potency was obtained in vascular conduits stimulated with endothelin-1. The rate of formation of immunoreactive 6-keto-PGF1 alpha under both normal and stimulated conditions by the inferior epigastric artery (normal, 301 +/- 8 pg/mg of tissue; stimulated, 519 +/- 15 pg/mg of tissue) was at 10 minutes more than 2 times (p < 0.01) that of the saphenous vein and about 1.5 times (p < 0.01) that of the right gastroepiploic artery.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cardiac Surgery, I. Monzino Fundation, Milan, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Sala
authors.forename: P
authors.initials: P
authors.lastname: Rona
authors.forename: G
authors.initials: G
authors.lastname: Pompilio
authors.forename: A
authors.initials: A
authors.lastname: Parolari
authors.forename: C
authors.initials: C
authors.lastname: Antona
authors.forename: P
authors.initials: P
authors.lastname: Biglioli
authors.forename: G
authors.initials: G
authors.lastname: Rossoni
authors.forename: L M
authors.initials: LM
authors.lastname: Villa
authors.forename: F
authors.initials: F
authors.lastname: Berti
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000009:Abdominal Muscles / Q000098:blood supply; D000368:Aged; D001158:Arteries / Q000378:metabolism / Q000637:transplantation; D001808:Blood Vessels / Q000378:metabolism* / Q000637:transplantation*; D016232:Endothelins / Q000494:pharmacology; D011464:Epoprostenol / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008323:Mammary Arteries / Q000378:metabolism / Q000637:transplantation; D008875:Middle Aged; D009204:Myocardial Revascularization; D012501:Saphenous Vein / Q000378:metabolism / Q000637:transplantation
chemical_list: D016232:Endothelins; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol