A new kind of immobilized lipase in organic solvent and its structure model.
In this paper, we used Ca-alginate gel beads coated with polyetheneimine and glutaraldehyde to adsorb Expansum penicillium lipase. The immobilized lipase catalyzed esterification of 1-dodecanol with dodecanoic acid in benzene. The results show that when the concentration of Ca-alginate, polyetheneimine (PEI) and glutaraldehyde is 1%, 6% and 1%, respectively, the activity of the immobilized lipase and the amount of adsorbed protein are the highest. The immobilized lipase is better than the SDS-immobilized lipase. The activity of the immobilized lipase connected by glutaraldehyde is higher than the activity of that without glutaraldehyde. The initial rate of the immobilized lipase and lyophilized lipase powder is 5.9 x 10(2) nmol/min.mgpr and 2.8 x 10(1) nmol/min.mgpr, respectively. After the immobilized lipase catalyzed the esterification reaction at 37 degrees C for about 12 hours, 93.3% of 1-dodecanol was converted to ester, but for lyophilized lipase powder, only 17.5% converted. Based on all above results, we have presumed and explained the structure of this kind of immobilized lipase.
Yang
H
H
National Laboratory of Enzyme Engineering, Jilin University, Changchun, China.
Cao
S G
SG
Ma
L
L
Ding
Z T
ZT
Liu
S D
SD
Cheng
Y H
YH
D000464:Alginates; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D020723:Glucuronic Acid; D005976:Glutaral; D006603:Hexuronic Acids; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D008961:Models, Structural; D010407:Penicillium / Q000201:enzymology; D011094:Polyethyleneimine; D012967:Sodium Dodecyl Sulfate; D012997:Solvents
D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D012997:Solvents; D012967:Sodium Dodecyl Sulfate; D020723:Glucuronic Acid; D011094:Polyethyleneimine; D008049:Lipase; D005976:Glutaral