Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes.
Flow cytometry and two-colour immunofluorescence were used to detect cytoplasmic bovine viral diarrhoea virus (BVDV) antigen in leukocytes from viraemic cattle. Monoclonal antibody to the p80 protein of BVDV, a non-structural viral antigen, was used to identify the subpopulations of leukocytes in which viral protein synthesis had occurred. Viral antigen was detected in 23% of peripheral blood mononuclear cells. Monocytes were found to have the highest frequency of infection (35%). A higher proportion of CD2+ T cells (23%) were infected, compared with B cells (11%) or WC1+ gamma delta T cells (11%). No significant differences in percentages of different leukocyte subpopulations in blood were detected in persistently viraemic animals compared with controls.
Sopp
P
P
AFRC Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, U.K.
Hooper
L B
LB
Clarke
M C
MC
Howard
C J
CJ
Brownlie
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood*; D002417:Cattle; D005434:Flow Cytometry; D007962:Leukocytes / Q000382:microbiology*; D009000:Monocytes / Q000382:microbiology; D017361:Viral Nonstructural Proteins / Q000097:blood*; D014766:Viremia
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017361:Viral Nonstructural Proteins