title: Effect of sulfur dioxide on mucociliary activity and ciliary beat frequency in guinea pig trachea.
abstract: The effects of 30 min exposure to sulfur dioxide on mucociliary activity (MCA) and ciliary beat frequency (CBF) were studied in 31 guinea pig tracheas. MCA was measured by recording the light reflected from ciliated mucous membranes using an infrared bar code reader. CBF of single ciliated cells obtained by brushing was measured with phase-contrast microscopy. Each tracheal sample was exposed to SO2 at concentrations ranging from 2.5 to 12.5 ppm, or to air for control purposes. MCA and CBF were measured before and immediately after gas exposure. A reduction in mean MCA of 63% (P = 0.0007) and statistically insignificant changes in CBF (P > 0.05) were recorded at concentrations of 2.5 ppm SO2. Higher SO2 concentrations caused a further impairment of MCA as well as a dose-dependent decrease in CBF (P = 0.002). A concentration of 12.5 ppm SO2 induced a decrease from baseline values of approximately 80% in mean MCA and of roughly 70% in mean CBF. This study demonstrates a dose-dependent SO2-induced decrease in MCA of guinea pig tracheas. The decrease in MCA was associated with an impairment of CBF only at SO2 concentrations higher than 5.0 ppm.
authors.affiliation: Third Department of Internal Medicine, Johannes Gutenberg University, Mainz, Germany.
authors.forename: M M
authors.initials: MM
authors.lastname: Knorst
authors.forename: K
authors.initials: K
authors.lastname: Kienast
authors.forename: H
authors.initials: H
authors.lastname: Riechelmann
authors.forename: J
authors.initials: J
authors.lastname: Müller-Quernheim
authors.forename: R
authors.initials: R
authors.lastname: Ferlinz
mesh_terms: D000818:Animals; D002925:Ciliary Motility Disorders / Q000139:chemically induced*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D009079:Mucociliary Clearance / Q000187:drug effects*; D013458:Sulfur Dioxide / Q000633:toxicity*; D014132:Trachea / Q000187:drug effects
chemical_list: D013458:Sulfur Dioxide