title: Specific binding of estrogen to osteoclast surfaces.
abstract: Specific plasma membrane binding sites for 17 beta-estradiol were shown to exist on avian osteoclasts by the use of a fluorescent conjugated estrogen, 17 beta-estradiol-6-carboxymethyloxime- bovine serum albumin-fluorescein isothiocyanate. Binding was dose and time dependent, as well as specific, since 17 beta-estradiol blocked the binding of the complex. Tamoxifen was also an effective blocking agent. Rapid responses to 17 beta-estradiol included decreased acid production (41.5%) and distinct cell shape changes.
authors.affiliation: Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802.
authors.forename: K D
authors.initials: KD
authors.lastname: Brubaker
authors.forename: C V
authors.initials: CV
authors.lastname: Gay
mesh_terms: D000143:Acids / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D048429:Cell Size / Q000187:drug effects; D002645:Chickens; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D004966:Estrogens, Conjugated (USP) / Q000378:metabolism / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011959:Receptors, Estradiol / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000143:Acids; D004966:Estrogens, Conjugated (USP); D011959:Receptors, Estradiol; C043089:estradiol 6-O-carboxymethyloxime-bovine serum albumin-fluorescein isothiocyanate; D012710:Serum Albumin, Bovine; D004958:Estradiol; D016650:Fluorescein-5-isothiocyanate