title: Regulation of isotonic shortening velocity by second messengers in tracheal smooth muscle.
abstract: Evidence suggests that the mechanical behavior of smooth muscle tissues is regulated by Ca(2+)-dependent changes in the phosphorylation of the 20,000-Da light chain of myosin (MLC). However, alternative mechanisms activated by specific kinases may be involved in regulating the shortening velocity in some smooth muscle tissues. To determine how the activation of protein kinases A or C affects the regulation of the shortening velocity in canine tracheal smooth muscle, we evaluated the effects of forskolin (10(-5) M) and phorbol 12,13-dibutyrate (PDBu, 3 x 10(-6) M) on active stress, intracellular Ca2+ ([Ca2+]i), MLC phosphorylation, and isotonic shortening velocity during contractions elicited by 60 mM KCl. Forskolin depressed and PDBu increased active stress, [Ca2+]i, MLC phosphorylation, and shortening velocity; thus the effects of these agents on the shortening velocity may result from changes in Ca(2+)-dependent MLC phosphorylation. In contrast, the decline in velocity that occurred with time during tonic contractions elicited by K+ depolarization was not associated with significant changes in MLC phosphorylation; thus the time-dependent changes in shortening velocity may be regulated by a mechanism other than MLC phosphorylation.
authors.affiliation: Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46202.
authors.forename: S J
authors.initials: SJ
authors.lastname: Gunst
authors.forename: M H
authors.initials: MH
authors.lastname: al-Hassani
authors.forename: L P
authors.initials: LP
authors.lastname: Adam
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D004285:Dogs; D004594:Electrophysiology; D007425:Intracellular Membranes / Q000378:metabolism; D007551:Isotonic Contraction; D009130:Muscle, Smooth / Q000502:physiology*; D009218:Myosins / Q000378:metabolism; D009994:Osmolar Concentration; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010766:Phosphorylation; D011189:Potassium Chloride / Q000494:pharmacology; D015290:Second Messenger Systems / Q000502:physiology*; D013314:Stress, Mechanical; D013997:Time Factors; D014132:Trachea / Q000502:physiology*
chemical_list: D005576:Colforsin; D015240:Phorbol 12,13-Dibutyrate; D011189:Potassium Chloride; D009218:Myosins; D002118:Calcium