The gradient of basic fibroblast growth factor concentration in human pancreatic cancer cell invasion.
It has been reported that basic fibroblast growth factor (b-FGF) is overexpressed in human pancreatic cancer cells, and that the overexpressed b-FGF contributes to the disease progression (Yamanaka et al. Cancer Res. 53, 5292-5297, 1993). Pancreatic cancer aggressively invades surrounding tissues by penetrating basement barriers. Thus, effect of b-FGF on pancreatic cancer, cell invasion to Matrigel basement membrane, was studied. The high gradient of b-FGF increased the invasiveness of human pancreatic cancer cells in a dose-dependent manner. Furthermore, the invasiveness of the cells was suppressed by anti-FGF receptor antibody. These results suggest that b-FGF plays an important role in human pancreatic cancer cell invasion to basement membrane.
Hasegawa
Y
Y
First Department of Surgery, Kobe University, School of Medicine, Japan.
Takada
M
M
Yamamoto
M
M
Saitoh
Y
Y
D003094:Collagen; D004338:Drug Combinations; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007797:Laminin; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000473:pathology*; D011509:Proteoglycans; D017468:Receptors, Fibroblast Growth Factor / Q000502:physiology; D014407:Tumor Cells, Cultured
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; C059485:matrigel; D003094:Collagen