Enrichment and characterization of dendritic cells from human bronchoalveolar lavages.
In the present study about 0.3% to 1.6% of human bronchoalveolar lavage (BAL) cells were identified as typical dendritic cells (DC), having an irregular outline, lobulated nucleus, and clear distinguishable acid phosphatase activity or EBM11 (anti-CD68) reactivity in a spot near the nucleus. After DC enrichment, using transient adherence to plastic, FcR-panning, and a density metrizamide gradient, a population containing 7-8% typical DC was obtained. This DC-enriched low density fraction, containing the highest percentages of DC, very strongly induced T cell proliferation in an allogeneic mixed leucocyte reaction (MLR), which was significantly higher than that induced by other partly (un)fractionated BAL cells. These data indicate that DC seem to be the major accessory cells in the BAL fluid, and therefore may be important in the regulation of T cell immune responses in the lung.
Havenith
C E
CE
Department of Cell Biology, Vrije Universiteit, Amsterdam, The Netherlands.
van Haarst
J M
JM
Breedijk
A J
AJ
Betjes
M G
MG
Hoogsteden
H C
HC
Beelen
R H
RH
Hoefsmit
E C
EC
D000135:Acid Phosphatase / Q000032:analysis; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012507:Sarcoidosis; D000097382:CD68 Molecule
D000135:Acid Phosphatase; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; C067980:CD68 antigen, human; D000097382:CD68 Molecule