Expression of acetylcholinesterase gene during in vitro differentiation of rabbit muscle satellite cells.
We investigated the myogenic properties and the expression of acetylcholinesterase (AChE) in culture of satellite cells (SCs) isolated from slow and fast rabbit muscles. Slow SCs form myotubes more rapidly (day 9 vs day 11) than fast SCs, and differentiate further into striated and contractile fibers. AChE activity and mRNA expression are higher in SCs cultured from slow than from fast muscles, as also observed in the muscles themselves. However, the two types of SC cultures do not show obvious difference in their patterns of AChE molecular forms. Taken together, these preliminary data support the view that there might be more than one SC population in skeletal muscles.
Barjot
C
C
Differenciation cellulaire et Croissance, INRA, Montpellier, France.
Jbilo
O
O
Chatonnet
A
A
Bacou
F
F
D000110:Acetylcholinesterase / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D009132:Muscles / Q000166:cytology / Q000201:enzymology*; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D011817:Rabbits
D007527:Isoenzymes; D012333:RNA, Messenger; D000110:Acetylcholinesterase