HLA antigens on leukocyte fragments and plasma proteins: prestorage leukoreduction by filtration.
HLA alloimmunization following blood transfusion results from recipient exposure to donor alloantigens. Numerous studies have documented that intact donor leukocytes are capable of provoking primary alloimmunization and that leukoreduction can decrease the incidence of primary HLA alloimmunization. HLA antigens also exist in soluble form and are present on leukocyte cell fragments. We measured the concentration of soluble HLA class I antigen in both standard and leukoreduced blood components during storage. Although the concentration of soluble class I HLA protein varied widely among different individuals, the concentration was stable during refrigerated storage of red cell concentrates and was not affected by leukocyte reduction by filtration. We also investigated whether or not HLA antigens present on leukocyte fragments were capable of stimulating either resting or in-vitro-primed lymphocytes in the mixed lymphocyte reaction (MLR).(ABSTRACT TRUNCATED AT 250 WORDS)
Dzik
S
S
Blood Bank and Tissue Typing Laboratory, Deaconess Hospital, Boston, MA 02215.
Szuflad
P
P
Eaves
S
S
D001793:Blood Preservation; D001798:Blood Proteins / Q000737:chemistry*; D002458:Cell Fractionation; D005374:Filtration; D006680:HLA Antigens / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007376:Interleukin-2 / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D011994:Recombinant Proteins / Q000494:pharmacology; D012995:Solubility; D065227:Transfusion Reaction
D001798:Blood Proteins; D006680:HLA Antigens; D007376:Interleukin-2; D007519:Isoantigens; D011994:Recombinant Proteins