Crystallization and preliminary X-ray analysis of a lipase from Bacillus subtilis.
Single crystals of the lipase from Bacillus subtilis have been obtained using a mixture of polyethylene glycol 4000 and sodium sulphate solution as the precipitant. The crystals grow at room temperature in two to three weeks in the presence of n-octyl-beta-D-glucoside. They belong to the monoclinic space group C2 with a = 121.20 A, b = 93.19 A, c = 80.96 A, and beta = 110.67 degrees, with four protein molecules per asymmetric unit. The crystals diffract to at least 2.5 A resolution and are suitable for an X-ray structure analysis.
Ransac
S
S
Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.
Blaauw
M
M
Lesuisse
E
E
Schanck
K
K
Colson
C
C
Dijkstra
B W
BW
D001412:Bacillus subtilis / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008049:Lipase / Q000737:chemistry*; D015394:Molecular Structure
D008049:Lipase