title: Polyvinylpyrrolidone-coated silica packings for chromatography of proteins and peptides.
abstract: Polyvinylpyrrolidone (PVP)-coated silica sorbents were synthesized by interaction of a copolymer of vinyl-methyldiethoxysilane and vinylpyrrolidone with LiChrospher Si 300 and LiChrospher Si 500 silicas. The coating procedure retains the wide-pore structure of the starting silicas, as was shown by nitrogen adsorption, mercury porosimetry and inverse size-exclusion chromatographic measurements. Good selectivity and separation ability of the synthesized packings toward proteins and peptides were demonstrated in the hydrophobic interaction chromatographic mode of separation. Aromatic compounds undergo a specific interaction with bonded PVP chains which can be used for the preconcentration or selective recovery of polycyclic aromatic hydrocarbons.
authors.affiliation: Institute of Organo-Element Compounds, Russian Academy of Sciences, Moscow.
authors.forename: A
authors.initials: A
authors.lastname: Kurganov
authors.forename: Y u
authors.initials: Yu
authors.lastname: Puchkova
authors.forename: V
authors.initials: V
authors.lastname: Davankov
authors.forename: F
authors.initials: F
authors.lastname: Eisenbeiss
mesh_terms: D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011083:Polycyclic Compounds / Q000302:isolation & purification; D011137:Polystyrenes; D016062:Porosity; D011205:Povidone / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide
chemical_list: D010455:Peptides; D011083:Polycyclic Compounds; D011137:Polystyrenes; D011506:Proteins; D012822:Silicon Dioxide; D011205:Povidone