title: Western blot and indirect fluorescent antibody testing for antibodies reactive with Ehrlichia canis in sera from apparently healthy dogs in Zimbabwe.
abstract: Sera were collected from apparently healthy dogs in 3 major centres in Zimbabwe to establish the prevalence of antibodies reactive with Ehrlichia canis by indirect fluorescent antibody (IFA) testing at titres of 1/20 or greater. The overall seroprevalence was 42% (39/93) with dogs from Mutare (40%) and Harare (33%) having similar rates which were lower than those for Bulawayo (68%). The majority of the IFA positive dogs from Harare (67%) had no haematological or biochemical evidence of canine ehrlichiosis. Total protein, albumin and globulin values were determined in sera from blood collected from Bulawayo and Mutare and all values were within normal reference ranges. Western blot profiles of sera from dogs with IFA titres of greater than 1/160 were similar to those using sera from dogs experimentally infected with E. canis Oklahoma strain. Our results indicate that an organism which is antigenically very similar or identical to E. canis is present in Zimbabwe and that dogs are commonly exposed to this organism.
authors.affiliation: Faculty of Veterinary Science, University of Zimbabwe, Mount Pleasant, Harare.
authors.forename: L A
authors.initials: LA
authors.lastname: Matthewman
authors.forename: P J
authors.initials: PJ
authors.lastname: Kelly
authors.forename: S M
authors.initials: SM
authors.lastname: Mahan
authors.forename: D
authors.initials: D
authors.lastname: Semu
authors.forename: M
authors.initials: M
authors.lastname: Tagwira
authors.forename: P A
authors.initials: PA
authors.lastname: Bobade
authors.forename: P
authors.initials: P
authors.lastname: Brouqui
authors.forename: P R
authors.initials: PR
authors.lastname: Mason
authors.forename: D
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D015153:Blotting, Western; D004283:Dog Diseases / Q000453:epidemiology* / Q000276:immunology; D004285:Dogs; D004536:Ehrlichia / Q000276:immunology; D016873:Ehrlichiosis / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D015995:Prevalence; D015030:Zimbabwe / Q000453:epidemiology
chemical_list: D000907:Antibodies, Bacterial