title: Two-color analysis of peripheral lymphocyte surface antigens in inherently healthy adults.
abstract: We studied 134 "healthy persons" with no past history of any serious or latent diseases, and observed differences in their 25 lymphocyte subsets by conducting two-color analyses using 12 useful combinations of dye-labeled monoclonal antibodies. CD4+Leu8+ cells and CD25+CD3+ cells were significantly higher in males than in females, whereas CD4+Leu8- cells and CD23+CD19+ cells were significantly higher in females. In comparison of the 25 lymphocyte subsets among four age groups, CD45RA-CD4+ cells and CDw29+CD4+ cells significantly increased with age, and CD8+ CD11b- cells and CD57-CD8+ cells significantly decreased with age. In females, helper T cells significantly increased and helper inducer T cells increased, while cytotoxic T cells decreased with age. These are important findings that should be considered in studies of immune function and autoimmune disorders.
authors.affiliation: Department of Laboratory Medicine, Mie University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamashiki
authors.forename: A
authors.initials: A
authors.lastname: Nishimura
authors.forename: Y
authors.initials: Y
authors.lastname: Kosaka
authors.forename: S P
authors.initials: SP
authors.lastname: James
mesh_terms: D000328:Adult; D000375:Aging / Q000276:immunology; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000097:blood* / Q000145:classification; D003124:Colorimetry / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012737:Sex Factors
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface