title: Alteration of tumorigenicity in undifferentiated thyroid carcinoma cells by introduction of normal chromosome 11.
abstract: The tumorigenicity of various cell lines has been shown to be suppressed by the introduction of chromosome 11 and other chromosomes via micro-cell-mediated chromosome transfer. In this study, we investigated whether a human undifferentiated thyroid carcinoma cell line, TTA-1, was suppressed by the introduction of normal human chromosome 11 or 10. Chromosome 10 or 11 was transferred from A9 cells containing a single human chromosome 10 or 11 tagged with pSV2-neo plasmid DNA into TTA-1 cells, by microcell fusion. The tumorigenicity of the TTA-1 cells and their colony-forming efficiency in soft agar were suppressed by chromosome 11, but not by chromosome 10. These results suggest that normal human chromosome 11 carries a putative tumor suppressor gene that affects the tumor-associated phenotypes of TTA-1 cells.
authors.affiliation: Department of Surgery, Kanagawa Cancer Center, Yokohama, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Asaga
authors.forename: C
authors.initials: C
authors.lastname: Masuzawa
authors.forename: S
authors.initials: S
authors.lastname: Kawahara
mesh_terms: D000818:Animals; D015197:Carcinogenicity Tests; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002879:Chromosomes, Human, Pair 10; D002880:Chromosomes, Human, Pair 11; D005260:Female; D018014:Gene Transfer Techniques; D016147:Genes, Tumor Suppressor; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured