title: Endocrine control of stress-induced heat shock protein 70 expression in vivo.
abstract: BACKGROUND
  Stress adaptation requires interactions between the hypothalamic-pituitary-adrenal axis, the sympathetic nervous system, and a family of intracellular stress response proteins termed heat shock proteins (HSPs). These HSPs are present in every living organism and are selectively induced in the adrenal cortex and vascular smooth muscle after either surgical or restraint stress.
  
  
  METHODS
  We perturbed the hypothalamic-pituitary-adrenal axis by implanting in the rat subcutaneous pellets containing either placebo or dexamethasone (25 mg), ovine corticotropin releasing factor (CRF, 0.5 mg), or the glucocorticoid antagonist RU 486 (5 mg) for 2 weeks before randomization to either 90 minutes of restraint stress or immediate sacrifice. The adrenal glands were weighed, trunk blood was collected for adrenocorticotropic hormone (ACTH) and corticosterone measurements, and RNA isolated from the adrenal glands and aorta was assayed for HSP70 messenger RNA expression by Northern analysis.
  
  
  RESULTS
  Dexamethasone resulted in a twofold decrease in adrenal weight (p < 0.05). ACTH and corticosterone levels were markedly reduced in the dexamethasone treated group in the absence or presence of restraint stress. Restraint resulted in greater than 20-fold induction of HSP70 in both the adrenal gland and aorta of the placebo group compared with nonstressed controls (p < 0.01). Long-term dexamethasone treatment reduced adrenal HSP70 expression fourfold after restraint (p < 0.5), whereas neither CRF nor RU486 treatment significantly influenced the adrenal HSP70 response. Glucocorticoid manipulation with either dexamethasone or CRF did not significantly affect restraint-induced aortic HSP70 expression, whereas RU486 treatment resulted in a 50% diminution (p < 0.5) compared with placebo-treated controls.
  
  
  CONCLUSIONS
  These data show dramatic induction of HSP70 messenger RNA expression in adrenal and aortic tissues after restraint stress. Differential organ specific HSP regulation is evidenced by the ability of the glucocorticoid dexamethasone to attenuate the adrenal but not the aortic response. The significant effect of RU486 on the aortic response suggests the possibility of vascular glucocorticoid-catecholamine interactions.
authors.affiliation: Laboratory of Endocrine Surgery, Johns Hopkins Hospital, Baltimore, MD 21287-5674.
authors.forename: R
authors.initials: R
authors.lastname: Udelsman
authors.forename: M J
authors.initials: MJ
authors.lastname: Blake
authors.forename: C A
authors.initials: CA
authors.lastname: Stagg
authors.forename: N J
authors.initials: NJ
authors.lastname: Holbrook
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002395:Catecholamines / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D003907:Dexamethasone / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology*; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D010913:Pituitary-Adrenal System / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000378:metabolism*
chemical_list: D002395:Catecholamines; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D015735:Mifepristone; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone