Influencing the influenza virus: genetic analysis and engineering of the negative-sense RNA genome.
The genome of influenza virus has become amenable to genetic analysis as a result of newly developed methods for reconstitution of the ribonucleoprotein (RNP) complex. This allows a dissection of the regulatory signals and promoters required for transcription and replication of the viral genome. The ability to rescue chimeric viruses in vivo after transfection opens up a way to engineer viruses suitable for human immunization and carrying desirable antigenic determinants.
Seong
B L
BL
Chemical Pathology Unit, Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
D001483:Base Sequence; D005818:Genetic Engineering; D016679:Genome, Viral; D006801:Humans; D007252:Influenza Vaccines / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000235:genetics* / Q000276:immunology; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014779:Virus Replication / Q000235:genetics
D007252:Influenza Vaccines; D016372:RNA, Antisense; D012367:RNA, Viral; D012261:Ribonucleoproteins