Does granulocyte depletion protect against ischaemic muscle necrosis?
Male Wistar rats were submitted to complete hindlimb ischaemia for 4.5 h. Six animals were injected with antineutrophilic serum (ANS) pre- and post-operatively, and eight animals received normal sheep serum (NSS). The rats were killed 72 h after the insult, and the areas of necrosis in the anterior tibial muscles were measured morphometrically on histological slides. In the NSS group there was a considerable postoperative increase in polymorphonuclear leukocytes (PMNLs) in peripheral blood, while treatment with ANS resulted in total depletion of PMNLs during ischaemia and early reperfusion. There was 31% necrosis in the NSS group and 28% necrosis in the ANS group. This difference was not significant (p = 0.7). It is concluded that depletion of PMNLs had no effect on the infarct size in the anterior tibial muscle after 4.5 h of complete hindlimb ischaemia and 72 h of reperfusion.
Skjeldal
S
S
Institute for Surgical Research, Rikshospitalet (The National Hospital), Oslo, Norway.
Grøgaard
B
B
Nordsletten
L
L
Torvik
A
A
Svindland
A
A
Reikarås
O
O
D000818:Animals; D006614:Hindlimb; D007106:Immune Sera / Q000494:pharmacology; D007511:Ischemia / Q000473:pathology*; D008297:Male; D009132:Muscles / Q000098:blood supply* / Q000473:pathology; D009336:Necrosis / Q000209:etiology / Q000517:prevention & control*; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D012756:Sheep / Q000097:blood
D007106:Immune Sera