ALATOP:sensitivity, specificity and predictive value of a new "in vitro" screening test of atopy.
The limitations of total IgE for the screening of atopy, gave rise to in vitro multispecific IgE tests to common allergens. These should have an increased sensitivity and specificity when compared to total IgE. Previous data showed the interest of such tests in screening procedures. ALATOP is a new in vitro multispecific IgE test including common aero and food allergens, in liquid phase, for paediatric and adult population. The result is expressed only in positive/negative. The aim of the study was to determine the sensitivity, specificity, positive and negative predictive value and test efficiency, by comparing the results obtained in 266 paediatric patients (selected in our Unit based on a good correlation between clinical history and skin prick tests--SPT), between ALATOP and SPT.


RESULTS
1) Sensitivity: 89.57%; 2) Specificity: 98.06%; 3) Positive predictive value: 98.65%; 4) Negative predictive value: 85.59%; 5) Efficiency of the test: 92.86%; 6) Reproducibility of the test--average: 5.63%. Based on this results and when they are compared to single specific IgE determinations, that are assumed to be about 80% of the sensitivity of SPT, ALATOP showed excellent results, allowing its use in the screening of atopic disease.
Nogueira
J M
JM
D. Estefânia Hospital, Lisbon, Portugal.
de Almeida
M M
MM
Fernandes
J G
JG
Santa Marta
C
C
Pinto
P L
PL
Pinto
J E
JE
D000293:Adolescent; D000485:Allergens; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D005260:Female; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007223:Infant; D007428:Intradermal Tests; D008297:Male; D008403:Mass Screening; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D012680:Sensitivity and Specificity
D000485:Allergens; D007073:Immunoglobulin E