Ascorbic acid-2-0-beta-glucuronide, a new metabolite of vitamin C identified in human urine and uremic plasma.
A new metabolite of ascorbic acid has been isolated by a multi-step chromatographic procedure both from normal human urine and uremic plasma. Nuclear Magnetic Resonance studies, and chemical and enzymic analyses indicated that the compound is a conjugated structure consisting of equimolar ascorbic and beta-D-glucuronic acids. We determined the pKa value of the ascorbic acid moiety of the compound on the basis of variations of ultraviolet absorbances as a function of pH. Results showed that glucuronic acid is coupled to the 2-position of ascorbic acid.
Gallice
P
P
Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.
Sarrazin
F
F
Polverelli
M
M
Cadet
J
J
Berland
Y
Y
Crevat
A
A
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D005965:Glucuronates / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D014511:Uremia / Q000097:blood* / Q000652:urine
D005965:Glucuronates; C086944:ascorbic acid-2-O-glucuronide; D001205:Ascorbic Acid