Morulae at compaction and the pattern of protein synthesis in mouse embryos.
Compaction of mouse embryos at the 8-cell stage causes a drastic change in cell form and in cell-to-cell contacts in 3-4 h. We have studied the effect of inhibitors of transcription (alpha-amanitin), DNA replication (aphidicolin) and compaction (cytochalasin D, EGTA, alpha-lactalbumin and Con A) on the pattern of protein synthesis using gel electrophoresis. Our results show that the pattern of protein synthesis is regulated principally by passage through S phase during each early cell cycle rather than by de novo transcription, while changes induced in cell form or contacts do not alter the pattern significantly.
Mayor
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R
Departamento de Biologia, Universidad de Chile, Santiago.
Izquierdo
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L
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects / Q000502:physiology; D004533:Egtazic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D005260:Female; D007768:Lactalbumin / Q000494:pharmacology; D008715:Methionine / Q000378:metabolism; D051379:Mice; D009028:Morula / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D014176:Protein Biosynthesis; D016196:S Phase; D013462:Sulfur Radioisotopes; D014158:Transcription, Genetic / Q000187:drug effects
D000546:Amanitins; D013462:Sulfur Radioisotopes; D003208:Concanavalin A; D015638:Cytochalasin D; D016590:Aphidicolin; D004533:Egtazic Acid; D004247:DNA; D007768:Lactalbumin; D008715:Methionine