title: Sex identification by polymerase chain reaction using a Y-autosome homologous primer set.
abstract: We have developed a one-step polymerase chain reaction (PCR) technique which detects a sequence on the human Y chromosome and an autosomal sequence in one reaction. The method is very reliable for the sex determination, as the detection of the autosome-specific signal ensures the presence of DNA in the specimen even in the absence of the Y-specific signal.
authors.affiliation: Department of Human Genetics, School of International Health, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: Y
authors.initials: Y
authors.lastname: Nakahori
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: O
authors.initials: O
authors.lastname: Tsutsumi
authors.forename: Y
authors.initials: Y
authors.lastname: Taketani
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagome
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites; D012732:Sex Determination Analysis / Q000379:methods*; D014998:Y Chromosome
chemical_list: D017931:DNA Primers