title: Glycosylphosphatidylinositol-anchored CD4 supports human immunodeficiency virus type 1 replication, but not cytopathic effect, in T-cell transfectants.
abstract: Despite equivalent p24 antigen production, HSB-2 T cells expressing glycosylphosphatidylinositol (GPi)-linked CD4 were productively infected without cell death or syncytium formation, unlike HSB-2 transfectants expressing wild-type CD4 (wtCD4). HSB-2 transfectants dually expressing wtCD4 and GPi-linked CD4 formed syncytia and died. Thus, wtCD4 expression is critical for human immunodeficiency virus cytopathic effect in HSB-2 transfectants.
authors.affiliation: Division of Infectious Disease, Dana-Farber Cancer Institute, Boston, MA 02115.
authors.forename: W L
authors.initials: WL
authors.lastname: Marshall
authors.forename: E S
authors.initials: ES
authors.lastname: Mittler
authors.forename: P
authors.initials: P
authors.lastname: Avery
authors.forename: J P
authors.initials: JP
authors.lastname: Lawrence
authors.forename: R W
authors.initials: RW
authors.lastname: Finberg
mesh_terms: D015704:CD4 Antigens / Q000378:metabolism*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D014162:Transfection; D014779:Virus Replication
chemical_list: D015704:CD4 Antigens; D017261:Glycosylphosphatidylinositols