In vivo incorporation of [2-14C]mevalonic acid into rat brain complex glycolipids: characterization of glycosylsterols in mammalian brain.
The in vivo incorporation of [2-14C]mevalonate into complex glycolipids of rat brain was investigated. Results indicated that the majority of the incorporated radioactivity was found to be in cholesterol. Analysis of neutral glycolipid and ganglioside fractions revealed that there were selected radioactive bands co-migrating to known glycolipids and gangliosides and the hydrolysis of which released the radioactive cholesterol. These results indicated the existence of glycosylsterols in the rat central nervous system.
Hungund
B L
BL
New York State Psychiatric Institute, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Dayal
B
B
Dayal
V K
VK
Salen
G
G
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D008297:Male; D008798:Mevalonic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D013261:Sterols / Q000737:chemistry / Q000378:metabolism
D006017:Glycolipids; D013261:Sterols; C036218:galactosylcholesterol; D002784:Cholesterol; D008798:Mevalonic Acid