Accelerated recovery from toxic acute renal failure with thyroxin: stimulation of renal phospholipid biosynthesis.
Thyroxine (T4) seems to accelerate recovery from various forms of acute renal failure. The mechanisms of this effect are still debated. We decided to evaluate if thyroxine enhances the recovery of HgCl2 renal failure through an increment in the mitotic activity or through an increase in membrane phospholipid biosynthesis of the regenerating tubular cells. Male Wistar rats were allocated to four groups: one group received 0.4 mg/100 g BW HgCl2 SC and saline IP (HgCl2 group); the second received the toxin and 24 and 48 h after it, T4 15 micrograms/100 g BW IP (HgCl2 + T4 group); a third group received saline SC and T4 IP (T4 group), and the last group received saline SC and IP (control group). On the third day GFR was evaluated by 24-h creatinine clearance and afterward rats were sacrificed and the kidneys removed. Some of them were studied histologically, evaluating the severity of the tubular lesion using a semiquantitative score (0-4) and the mitotic index (N mitotic figures per 10 high-power fields). In the other kidneys we studied phospholipid synthesis through the incorporation of 32 P into the different renal phospholipids of the several kidney regions. The T4-treated group had a better recovery of GFR after the toxin (HgCl2 + T4: 0.44 +/- .09 vs. HgCl2: 0.23 +/- .06, p < .05). Both HgCl2-treated groups had similar lesional scores and mitotic indexes.(ABSTRACT TRUNCATED AT 250 WORDS)
Negri
A L
AL
Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Alvarez
C
C
Fernandez
M C
MC
Kane
L
L
Sterin-Speziale
N
N
Arrizurieta
E
E
D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology; D008297:Male; D008627:Mercuric Chloride / Q000506:poisoning; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013974:Thyroxine / Q000627:therapeutic use*
D010713:Phosphatidylcholines; D008627:Mercuric Chloride; D013974:Thyroxine