Effects of macromolecular chelating agents on the release of 239Pu and 59Fe from rat alveolar macrophages after phagocytic uptake of 239Pu-59Fe-iron hydroxide colloid.
The chelation-mediated mobilization of 239Pu and 59Fe from rat alveolar macrophages which had engulfed 239Pu-59Fe-hydroxide colloids was studied by exposing the macrophages to diethylenetriaminepentaacetic acid and macromolecular weight forms of diethylenetriaminepentaacetic acid. The release of 239Pu and 59Fe by these macromolecular weight chelating agents and diethylenetriaminepentaacetic acid was assessed in monolayer culture and shown to be dependent upon the nature of the engulfed colloids. Whereas calcium-diethylenetriaminepentaacetic acid, aminoethylcellulose-diethylenetriaminepentaacetic acid, and aminoethylcellulose-diethylenetriaminepentaacetic acid oxidized with periodic acid (aminoethylcellulose-diethylenetriaminepentaacetic acid(I2) exhibited some effectiveness at releasing 239Pu and 59Fe from macrophages which had engulfed the low aggregative colloid, they were less effective in the release of these radionuclides from the high aggregative colloid. The efficacy of these chelating agents on the removal of radionuclides from alveolar macrophages differed between iron and plutonium, suggesting the significant difference in the dissolution and subsequent metabolism of these radionuclides in alveolar macrophages. From the similarities of the percentage increases in 239Pu mobilized from the low aggregate colloid by aminoethylcellulose-diethylenetriaminepentaacetic acid (I2) and diethylenetriaminepentaacetic acid, it would appear that both forms of diethylenetriaminepentaacetic acid could be accessing the same deposits of 239Pu; however, for 59Fe the percentage increases are quite different for the two forms of diethylenetriaminepentaacetic acid. SiO2-diethylenetriaminepentaacetic acid, an insoluble macromolecular weight chelating agent, was ineffective at releases 239Pu but was slightly effective at releasing radioiron.
Sato
H
H
Division of Comparative Radiotoxicology, National Institute of Radiological Sciences, Chibashi, Japan.
Bulman
R A
RA
Takahashi
S
S
Kubota
Y
Y
D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D005260:Female; D005290:Ferric Compounds / Q000378:metabolism*; D007504:Iron Radioisotopes / Q000378:metabolism*; D046911:Macromolecular Substances; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D010586:Phagocytes / Q000378:metabolism*; D011005:Plutonium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D002614:Chelating Agents; D005290:Ferric Compounds; D007504:Iron Radioisotopes; D046911:Macromolecular Substances; C021024:ferric hydroxide; D011005:Plutonium