title: Regulation of postendocytic trafficking of the epidermal growth factor receptor through endosomal retention.
abstract: Little is known about the regulation of EGF receptor (EGF-R) trafficking following endocytosis. We investigated this by using a series of EGF-R with altered cytoplasmic tails and comparing their ability to undergo recycling and lysosomal targeting in both the occupied and empty state. We found that 2-3% of empty EGF-R are internalized each minute, but rapidly recycle (t1/2 approximately 5 min). This constitutive internalization and recycling of empty receptors was independent of cytoplasmic receptor sequences. Occupied EGF-R, in contrast, displayed a much slower rate of recycling (t1/2 between 10-23 min) due to retention within recycling endosomes. Endosomal retention of different EGF-R correlated with lysosomal targeting of EGF. Intrinsic receptor tyrosine kinase activity had no discernible effect on postendocytic trafficking of EGF. Although sequences within the cytoplasmic tail of the EGF-R appear to be required for occupancy-dependent endosomal retention, they are distinct from those required for ligand-induced endocytosis. Our studies indicate that intracellular trafficking of the EGF-R is regulated by endosomal components that preferentially recognize occupied receptors. Down-regulation of the EGF-R thus involves two distinct regulatory processes: one at the level of internalization and one at the level of recycling.
authors.affiliation: Department of Pathology, University of Utah Medical Center, Salt Lake City 84132.
authors.forename: J J
authors.initials: JJ
authors.lastname: Herbst
authors.forename: L K
authors.initials: LK
authors.lastname: Opresko
authors.forename: B J
authors.initials: BJ
authors.lastname: Walsh
authors.forename: D A
authors.initials: DA
authors.lastname: Lauffenburger
authors.forename: H S
authors.initials: HS
authors.lastname: Wiley
mesh_terms: D000818:Animals; D001692:Biological Transport; D002458:Cell Fractionation; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004705:Endocytosis; D066246:ErbB Receptors / Q000378:metabolism*; D007700:Kinetics; D007739:L Cells; D051379:Mice; D015388:Organelles / Q000378:metabolism*
chemical_list: D066246:ErbB Receptors