Twenty-one polymorphic markers from human chromosome 12 for integration of genetic and physical maps.
Twenty-one physically mapped, polymorphic markers have been developed from a chromosome 12-specific cosmid library. The markers consist of CA repeat-containing sequence-tagged sites (STSs) derived from cosmid clones mapped by fluorescence in situ hybridization (FISH). Three methods for determining the sequence flanking CA microsatellites were used, including one using degenerate primer sets for direct sequence analysis. Oligonucleotide primer pairs suitable for use in polymerase chain reaction (PCR) were selected from the sequences flanking the CA microsatellite and were tested for their ability to generate unique PCR products. The informativeness of these STSs as genetic markers was determined by typing 10 unrelated individuals who are part of the Centre d'Etude du Polymorphisme Humaine (EPH) pedigrees. Eleven of the 21 FISH-mapped, polymorphic STSs are heterozygous in 7 or more of the individuals tested. Since these markers are derived from physically mapped cosmids, genetic linkage analysis with them will facilitate the integration of the developing physical and genetic maps of chromosome 12.
LeBlanc-Straceski
J M
JM
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461.
Montgomery
K T
KT
Kissel
H
H
Murtaugh
L
L
Tsai
P
P
Ward
D C
DC
Krauter
K S
KS
Kucherlapati
R
R
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D003360:Cosmids; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers