Is the IFN-gamma-induced enhancement of superoxide production in CGD-phagocytes caused by increased expression of the p47-phox cytosolic protein.
Interferon-gamma (IFN-gamma) had been shown to increase superoxide production of cultured monocytes from patients with chronic granulomatous disease (CGD). To elucidate the mechanism of the IFN-gamma-induced improvement of superoxide production of cultured monocytes from patients with CGD we examined the influence of IFN-gamma on the expression and the activity of the NADPH-oxidase in the monocytic cell-line Mono Mac 6. After cultivation of Mono Mac 6-cells in the presence of 500 U/ml IFN-gamma the superoxide production was found to be increased as well as the expression of the p47-phox cytosolic protein of the phagocytic NADPH-oxidase.
Jendrossek
V
V
Universitätskinderklinik Göttingen.
Damm
U
U
Liese
J
J
Belohradsky
B H
BH
Gahr
M
M
D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D005808:Genes, Recessive; D006105:Granulomatous Disease, Chronic / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000172:deficiency / Q000378:metabolism*; D019255:NADPH Oxidases; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010750:Phosphoproteins / Q000172:deficiency / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D010750:Phosphoproteins; D013481:Superoxides; D007371:Interferon-gamma; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D009252:NADPH Dehydrogenase