Primed in situ DNA amplification (PIDA).
A novel PCR in situ protocol is described which can be implemented in one day. An initial amplification step with a single primer pair is followed by an amplification with an internal oligonucleotide probe with labeled nucleotides (dig-dUTP). Detection is then accomplished with an antidigoxigenin antibody and substrate solution. This rapid and sensitive method may be useful in diagnosing low copy DNA in tissue specimens and cells.
Moss
R B
RB
Allergic Diseases Section, NIAID, National Institute of Health, Bethesda, Maryland.
Kaliner
M A
MA
D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005784:Gene Amplification; D006678:HIV / Q000235:genetics* / Q000302:isolation & purification*; D006679:HIV Seropositivity / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods* / Q000706:statistics & numerical data; D008198:Lymph Nodes / Q000382:microbiology; D015336:Molecular Probe Techniques; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004279:DNA, Viral