Two structural domains of initiation factor eIF-4B are involved in binding to RNA.
Translation initiation factor eIF-4B promotes the binding of mRNA to 40 S preinitiation complexes and together with eIF-4A possesses RNA helicase activity. To elucidate structural features involved in its function, a series of internal and C-terminal deletions, as well as point mutations, were constructed in the eIF-4B cDNA. The mutated cDNAs were expressed in transiently transfected COS-1 cells, and mutant forms of the factor were overproduced up to about 25-fold over endogenous eIF-4B levels. Inhibition of dihydrofolate reductase (DHFR) synthesis by high levels of eIF-4B variants was determined in vivo, and the binding of the eIF-4B forms to biotinylated RNA was measured in vitro. The results indicate that the N-terminal region containing the RNA binding motif with its RNP1 and RNP2 consensus elements is sufficient for inhibition of DHFR synthesis. Deletion of the RNP1 sequence abrogates RNA binding, but amino acid substitutions at conserved residues do not always inhibit RNA binding. Deletion of the DRYG domain near the middle of eIF-4B results in inhibition of RNA binding, but not of DHFR synthesis. Up to 164 residues of the C terminus are not required for RNA binding, but removal of 226 or more residues completely inhibits RNA binding, perhaps by the loss of two arginine-rich regions. The results suggest that both the RNA recognition motif and the arginine-rich region are required for stable RNA binding but that both are not necessary for in vivo inhibition of protein synthesis.
Naranda
T
T
Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
Strong
W B
WB
Menaya
J
J
Fabbri
B J
BJ
Hershey
J W
JW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002522:Chlorocebus aethiops; D016384:Consensus Sequence; D039601:Eukaryotic Initiation Factor-4A; D039642:Eukaryotic Initiation Factors; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010448:Peptide Initiation Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017354:Point Mutation; D012333:RNA, Messenger / Q000378:metabolism*; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D017384:Sequence Deletion; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis; D014162:Transfection
D039642:Eukaryotic Initiation Factors; D009838:Oligodeoxyribonucleotides; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012261:Ribonucleoproteins; C031799:eIF-4B; D013762:Tetrahydrofolate Dehydrogenase; D039601:Eukaryotic Initiation Factor-4A