title: Presence of autologous neutralizing antibodies against cytomegalovirus (CMV) in serum of human immunodeficiency virus type 1-infected patients shedding CMV in saliva.
abstract: This study evaluated whether cytomegalovirus (CMV) neutralizing capacity affected shedding of CMV in saliva in human immunodeficiency virus type 1 (HIV-1)-infected patients and mapped specific epitope reactivity of CMV IgG antibodies. Total CMV IgG titers were significantly higher in symptomatic than in asymptomatic HIV-1-infected patients or controls. All CMV-seropositive patients had neutralizing antibodies to CMV. Shedding of CMV in the saliva of AIDS patients occurs despite the presence of serum antibodies with a high capacity to neutralize autologous CMV isolates. The highest IgG reactivity against a CMV envelope protein (gp116), represented by a peptide, was found in patients with advanced HIV disease. In contrast, identical IgG reactivities against a peptide representing the CMV matrix protein were observed in healthy controls and HIV-1-infected persons.
authors.affiliation: Dept. of Virology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Lucht
authors.forename: V A
authors.initials: VA
authors.lastname: Sundqvist
authors.forename: A
authors.initials: A
authors.lastname: Linde
authors.forename: G
authors.initials: G
authors.lastname: Bratt
authors.forename: E
authors.initials: E
authors.lastname: Sandström
authors.forename: A
authors.initials: A
authors.lastname: Heimdahl
authors.forename: C E
authors.initials: CE
authors.lastname: Nord
authors.forename: B
authors.initials: B
authors.lastname: Wahren
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications / Q000276:immunology* / Q000382:microbiology; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012463:Saliva / Q000382:microbiology*; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral