[Use of the polymerase chain reaction for studying the processes of Salmonella typhimurium cells to an noncultivated state].
The possibility to identify noncultivating forms of Salmonella by the polymerase chain reaction (PCR) has been shown. To do it the technique for Salmonella identification was elaborated, based on amplification of a 500 bp fragment of araC gene. Time course of populations of two Salmonella typhimurium strains during prolonged incubation in water was studied by the techniques of serial dilutions on solid nutrient media, acridine orange staining, and PCR. The strains differed in pathogenicity levels and genetic characteristics. Cells of nonvirulent strain were shown to loose gradually in the process of incubation the ability to grow on solid nutrient media and transform into noncultivating forms whose ability to proliferation can be restored under definite conditions. No difference in the dynamics identified by PCR or traditional microbiological techniques was found for population of virulent Salmonella typhimurium incubated in water indicating the possibility of fast degradation of cells having lost the ability to divide.
Aksenov
M Iu
MIu
Garovnikova
Iu S
IuS
Levina
G A
GA
Romanova
Iu M
IuM
Gintsburg
A L
AL
Prozorovskiĭ
S V
SV
D001483:Base Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D013045:Species Specificity
D004269:DNA, Bacterial
Ispol'zovanie metoda polimeraznoĭ tsepnoĭ reaktsii dlia izucheniia protsessa perekhoda kletok Salmonella typhimurium v nekul'tiviruemoe sostoianie.