Flow cytometric detection of DNA tumor virus nuclear oncogene products in unfixed cells: saponin FACS of viral oncogene products.
Immunofluorescent analysis is a standard method for detecting DNA virus oncoproteins in transformed cells. Here we demonstrate the detection of DNA virus nuclear oncoproteins by flow cytometry of unfixed cells, after saponin permeabilization. This method could to be of value in the evaluation and quantitation of oncogene products in transformed cells.
Eyler
Y L
YL
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Lantz
L M
LM
Lewis
A M
AM
D017498:Adenovirus E1 Proteins / Q000032:analysis; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability / Q000187:drug effects; D002467:Cell Nucleus / Q000737:chemistry; D005434:Flow Cytometry; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D011419:Propidium; D012503:Saponins / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D017498:Adenovirus E1 Proteins; D000952:Antigens, Polyomavirus Transforming; D009856:Oncogene Proteins, Viral; D012503:Saponins; D016159:Tumor Suppressor Protein p53; D011419:Propidium