Transcriptional activation by CTF proteins is mediated by a bipartite low-proline domain.
Members of the CCAAT-binding transcription factor (CTF) family of proteins stimulate the initiation of adenovirus DNA replication and act as transcriptional activators. To investigate the mechanisms underlying CTF-mediated transactivation patterns, we expressed several natural CTF variants in Saccharomyces cerevisiae and determined their transactivating activities in enzymatic assays. CTF7, which lacks the entire proline-rich region previously thought to mediate transcriptional activation by CTF proteins, enhances transcription to a greater degree than full-length CTF1, which contains the putative activation domain. CTF2, which contains a partially deleted proline-rich activation region, does not stimulate transcription at all. These findings indicate that the proline-rich region of CTF proteins is not essential for transcriptional activation in yeast. Our studies also suggest a bipartite two-domain structure of CTF-type transcriptional activation domains.
Altmann
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Institut für Biochemie der Ludwig-Maximilians-Universität, Martinsried, Germany.
Wendler
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Winnacker
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D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D025542:Neurofibromin 1; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics; D011392:Proline; D011506:Proteins / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013329:Structure-Activity Relationship; D013552:Swine; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D022762:CCAAT-Enhancer-Binding Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D025542:Neurofibromin 1; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011392:Proline