[Use of diethylenetriaminochloroplatinum in hybridization analysis of specific nucleic acid sequences].
Monofunctional platinum compound [Pt(dien)Cl] Cl and high-affinity antibodies against DNA-[Pt(dien)Cl]Cl were suggested for non-radioactive hybridisation analysis of DNA. The simple labelling procedure was based on mixing the reagents followed by the incubation for 2 h at 60 degrees C. The sensitivity and specificity of the technique were sufficient to detect 10 fg DNA in dot-hybridisation without cross-reaction with 10-fold excess of a heterologous DNA. This technique permits genome libraries screening.
Kiseleva
V I
VI
Turchinskiĭ
M F
MF
Kolesnik
T B
TB
Poverennyĭ
A M
AM
D000915:Antibody Affinity; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009944:Organoplatinum Compounds / Q000737:chemistry* / Q000276:immunology; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D009944:Organoplatinum Compounds; C032799:chlorodiethylenetriamine platinum; D004247:DNA; D002945:Cisplatin
Ispol'zovanie diétilentriaminkhlorplatiny v gibridizatsionnom analize spetsificheskikh posledovatel'nosteĭ nukleinovykh kislot.