[Further report on application of DNA probe in diagnosis of vivax malaria infection].
Southern blotting with a labeled and linearized pUC 19 DNA containing a specific fragment of 0.24 kb DNA of Plasmodium vivax asexual blood stages (kindly offered by Dr. C. Kidson) was used for further identification of blood samples showing positive reaction by dot-blot hybridization. The results showed that those with positive reaction from patients with P. vivax, with P. falciparum or with fever but with negative microscopic findings were also positive by Southern blotting. It was confirmed that some of those positive with P. falciparum were likely to infect P. vivax at the same time. So did a part of those with fever but negative in the blood films (Figs. 1,2).
Zhang
L X
LX
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Chai
J H
JH
Zhan
B
B
Feng
X P
XP
D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis; D006801:Humans; D016778:Malaria, Falciparum / Q000235:genetics; D016780:Malaria, Vivax / Q000175:diagnosis*; D010966:Plasmodium vivax / Q000235:genetics*
D015342:DNA Probes; D016054:DNA, Protozoan