Protein kinase C isoforms in murine erythroleukemia cells and their involvement in the differentiation process.
In addition to alpha, delta and epsilon-protein kinase C, murine erythroleukemia cells contain zeta-PKC and also a c-PKC isoform, named alpha 1, which shows cross-reactivity with an anti-alpha-PKC antipeptide antibody. In a C44 MEL cell clone, characterized by a high rate of differentiation, both c-PKC forms are expressed at a level higher than that of the N23 MEL cell clone which differentiates at a low rate and contains higher levels of epsilon-PKC and particularly of the delta-PKC isozyme. In the course of MEL cell differentiation, delta-PKC in N23 cells and alpha 1-PKC in C44 cells are rapidly down-regulated and the overall process is almost completed before cell commitment. Of the other three PKC isozymes present in both clones, only alpha-PKC is down-regulated to a significant extent. It is proposed that modulation of the signal delivered by each PKC isozyme is one of the biochemical mechanisms involved in MEL cell differentiation.
Patrone
M
M
Institute of Biochemistry, University of Genoa, Italy.
Pessino
A
A
Passalacqua
M
M
Sparatore
B
B
Melloni
E
E
Pontremoli
S
S
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002848:Chromatography, DEAE-Cellulose; D015151:Immunoblotting; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology*; D051379:Mice; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000081:Acetamides; D007527:Isoenzymes; D011493:Protein Kinase C; C014026:hexamethylene bisacetamide