title: CD70 represents the human ligand for CD27.
abstract: The recently identified CD27 ligand (L) is a type II transmembrane molecule with significant structural homology to tumor necrosis factor (TNF)-alpha, TNF-beta, lymphotoxin beta, CD40L, and CD30L. Using a CD27L specific mAb we examined the tissue distribution of the molecule, and found its expression to be restricted to B cells in occasional germinal centers, stromal cells in the thymic medulla, and scattered T cells in tonsils, skin and gut. As the limited expression of CD27L closely resembled the reported distribution of the activation antigen CD70, we tested whether CD70 represents the human CD27L. CD70 mAb were found to react with CD27L-expressing transfected mouse fibroblasts. Moreover a number of CD70 mAb could specifically interfere with the cellular binding of CD27L mAb. Thus, CD70 is identical to the human CD27L.
authors.affiliation: Department of Clinical (Viro-) Immunology, Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: R Q
authors.initials: RQ
authors.lastname: Hintzen
authors.forename: S M
authors.initials: SM
authors.lastname: Lens
authors.forename: G
authors.initials: G
authors.lastname: Koopman
authors.forename: S T
authors.initials: ST
authors.lastname: Pals
authors.forename: H
authors.initials: H
authors.lastname: Spits
authors.forename: R A
authors.initials: RA
authors.lastname: van Lier
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D001667:Binding, Competitive; D053282:CD27 Ligand; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D008024:Ligands; D008214:Lymphocytes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D053282:CD27 Ligand; C505771:CD70 protein, human; C505772:Cd70 protein, mouse; D008024:Ligands; D008565:Membrane Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7