Mechanism of hemolysis of canine erythrocytes induced by L-sorbose.
The cause of species difference in the susceptibility of erythrocytes to L-sorbose, and the difference in the hemolytic effect of sorbose on high potassium-containing (HK) and low potassium-containing (LK) canine erythrocytes were examined. L-Sorbose was phosphorylated in canine erythrocytes, but not in human erythrocytes. Furthermore, sorbose-1-phosphate, a metabolite of L-sorbose, strongly inhibited the hexokinase of LK canine erythrocytes, but not that of HK canine erythrocytes. These results strongly indicated that inhibition of hexokinase by sorbose-1-phosphate in LK erythrocytes induced severe glycolytic limitation in these cells, resulting in hemolysis, and that HK erythrocytes are resistant to sorbose-induced hemolysis because these cells have a high hexokinase activity.
Goto
I
I
Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Inaba
M
M
Shimizu
T
T
Maede
Y
Y
D000818:Animals; D004285:Dogs; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D006461:Hemolysis / Q000187:drug effects*; D006593:Hexokinase / Q000037:antagonists & inhibitors / Q000097:blood; D006600:Hexosephosphates / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D010766:Phosphorylation; D011188:Potassium / Q000097:blood; D013013:Sorbose / Q000031:analogs & derivatives / Q000097:blood / Q000494:pharmacology*
D006600:Hexosephosphates; C087061:sorbose-1-phosphate; D006593:Hexokinase; D013013:Sorbose; D011188:Potassium