Serum from patients with threatened abortion. Effect on in vitro development of mouse embryos.
The effect of serum from patients with threatened abortion on in vitro development of two-cell mouse embryos was evaluated. Embryos were cultured for 72 hours in 7.5% serum supplement from patients with threatened abortion and from women with normal pregnancies and in Ham's F-10 medium with no serum supplement. Significantly fewer embryos developed to the blastocyst stage at 72 hours in the threatened abortion group as compared with the normal pregnancy group and Ham's F-10 (P < .001). These results suggest the presence of serum factors that are toxic to early embryonic development and thus may play a role in the pathogenesis of threatened abortion.
Abu-Musa
A
A
Department of Obstetrics and Gynecology, Shimane Medical University, Izumo, Japan.
Takahashi
K
K
Okada
S
S
Sakoda
R
R
Kitao
M
M
D000033:Abortion, Threatened / Q000097:blood* / Q000276:immunology; D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood; D001681:Biological Assay; D001755:Blastocyst / Q000502:physiology*; D002970:Cleavage Stage, Ovum / Q000502:physiology*; D004195:Disease Models, Animal; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D011256:Pregnancy Outcome; D013997:Time Factors
D000974:Antibodies, Antinuclear