title: Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors.
abstract: The molecular characterization of a recurring complex chromosomal translocation involving 6p21, 6p22, 6q23, and 11q13 in two independent but similar extragonadal human germ cell tumors was initiated using fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis (PFGE) techniques. By using a series of specific probes from the 11q13 region, the translocation breakpoint in this chromosomal band could be located within a long-range restriction enzyme map in between the markers D11S457 and D11S546. In addition, aberrantly hybridizing restriction fragments were revealed by PFGE in both tumors, indicating that the breakpoint region must be located within a distance of at maximum 200 kilobase pairs (kbp) from the nearest DNA marker (D11S546).
authors.affiliation: Department of Human Genetics, University Hospital, Nijmegen, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Sinke
authors.forename: D O
authors.initials: DO
authors.lastname: Weghuis
authors.forename: R F
authors.initials: RF
authors.lastname: Suijkerbuijk
authors.forename: A
authors.initials: A
authors.lastname: Tanigami
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: C
authors.initials: C
authors.lastname: Larsson
authors.forename: G
authors.initials: G
authors.lastname: Weber
authors.forename: B
authors.initials: B
authors.lastname: de Jong
authors.forename: J W
authors.initials: JW
authors.lastname: Oosterhuis
authors.forename: W M
authors.initials: WM
authors.lastname: Molenaar
mesh_terms: D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D002896:Chromosomes, Human, Pair 6; D015342:DNA Probes; D016521:Electrophoresis, Gel, Pulsed-Field; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008479:Mediastinal Neoplasms / Q000235:genetics*; D012186:Retroperitoneal Neoplasms / Q000235:genetics*; D013724:Teratoma / Q000235:genetics*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D005819:Genetic Markers