Influenza virus M2 protein modifies membrane permeability in E. coli cells.
The M2 protein of influenza virus is an integral membrane protein with ion channel activity. This protein has been expressed in E. coli cells in an inducible manner. Expression of the M2 protein causes rapid lysis of BL21(DE3) pLysS E. coli cells upon induction with IPTB. M2 protein increases membrane permeability to a number of hydrophylic molecules, such as ONPG, uridine or impermeant translation inhibitors. The behaviour of M2 in bacteria resembles that of other viral proteins, such as poliovirus 3A and Semliki Forest virus 6K.
Guinea
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Centro de Biologia Molecular, CSIC-UAM, Universidad Autónoma de Madrid, Canto Blanco, Spain.
Carrasco
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D002463:Cell Membrane Permeability; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D009980:Influenza A virus; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D014763:Viral Matrix Proteins / Q000235:genetics / Q000494:pharmacology*; D000086082:Viroporin Proteins
D007544:Isopropyl Thiogalactoside; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; C031540:M-protein, influenza virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins