Use of the translational mobility of a plasma membrane protein to assess fertilization of mouse oocytes and viability of mouse zygotes and two-cell embryos.
The fluorescence photobleaching recovery (FPR) technique was used to measure the translational mobility of a glycoprotein recognized by the monoclonal antibody (mAb) S75 in plasma membranes of mouse oocytes, zygotes, and two-cell embryos. Glycoprotein fractional mobility (f) was significantly decreased in membranes of unfertilized oocytes compared to zygotes or two-cell embryos (f values, 46 +/- 2 and 65 +/- 2%, respectively; p < 0.0001). Reduced apparent glycoprotein mobility was also observed in morphologically degenerated zygotes and two-cell embryos compared to viable zygotes and two-cell embryos (f values, 8 +/- 1 and 60 +/- 3%, respectively; p < 0.0001). These results indicate that the FPR technique can be used to assess oocyte fertilization and preimplantation embryonic viability. This method may be useful in the evaluation of embryonic viability following in vitro fertilization and in the detection of toxic effects of novel compounds on embryonic development.
Polgár
K
K
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Boston, Massachusetts.
Yacono
P W
PW
Hill
J A
JA
Anderson
D J
DJ
Lee
C Y
CY
Golan
D E
DE
D000818:Animals; D000911:Antibodies, Monoclonal; D001755:Blastocyst / Q000502:physiology*; D005260:Female; D005306:Fertilization; D005328:Fetal Viability; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D010777:Photochemistry; D015053:Zygote / Q000502:physiology*
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D008562:Membrane Glycoproteins; D008563:Membrane Lipids; D016650:Fluorescein-5-isothiocyanate