Modulation of tumour associated antigen expressed on human squamous cell carcinoma cell lines by recombinant interferon-alpha.
Our earlier studies have shown that monoclonal antibody (Mab) 3F8E3 generated against a head and neck cancer cell line LICR-LON-HN2 showed reactivity with squamous cell carcinoma (SCC) irrespective of the tissue of origin and identified antigens on SCC cell lines AW 13516 and AW 8507. The affinity constants (Ka) for binding of Mab 3F8E3 to AW 13516 and AW 8507 cell lines were 6.2 x 10(8) and 4.3 x 10(8) mol/l and it identified 6.8 x 10(4) and 3.77 x 10(4) sites/cell, respectively, as determined by Scatchard analysis. Treatment of both the cell lines with recombinant human interferon-alpha (rHu-IFN alpha) increased the binding affinity of the Mab but did not increase the number of binding sites on the SCC cell lines. Shedding of antigen recognised by the Mab in the culture supernatant of the cell lines was reduced after rHu-IFN alpha treatment. The results suggest that rHu-IFN alpha may bring about a firm anchorage of the tumour associated antigen on the SCC cells. Cells modulated with rHu-IFN alpha may serve as better targets for assessing cell mediated as well as Mab mediated cytotoxicity in oral cancer patients.
Maniar
H S
HS
Immunology Division, Cancer Research Institute, Bombay, India.
Desai
S A
SA
Chiplunkar
S V
SV
Amin
M K
MK
Rao
R S
RS
Gangal
S G
SG
D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000951:Antigens, Neoplasm / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D001665:Binding Sites; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002417:Cattle; D006801:Humans; D007370:Interferon Type I / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D011994:Recombinant Proteins; D014062:Tongue Neoplasms / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007370:Interferon Type I; D011994:Recombinant Proteins