Cloning and sequence analysis of the gene encoding the low potential cytochrome c of Synechocystis PCC 6803.
The gene for the low potential cytochrome c (petK) was isolated from a genomic library of Synechocystis PCC 6803. The nucleotide sequence of this gene contains two regions with sequence similarity to two regions in the gene for the high potential cytochrome c6 of the organism. The sixth iron ligand can be identified with a conserved histidine. Experiments demonstrate the reduction of the low potential cytochrome by reduced ferredoxin II. The heme of the cytochrome is flanked by lysines which may be involved in orienting the ferredoxin near the site of electron donation.
Kang
C
C
Biochemistry Department, Purdue University, W. Lafayette, IN 47907.
Chitnis
P R
PR
Smith
S
S
Krogmann
D W
DW
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000737:chemistry / Q000235:genetics*; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics*; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism; D005288:Ferredoxins / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017421:Sequence Analysis; D017385:Sequence Homology
D003574:Cytochrome c Group; D005288:Ferredoxins; D005287:Ferredoxin-NADP Reductase