title: The promiscuous plasmids pIP501 and pAM beta 1 from gram-positive bacteria encode complementary resolution functions.
abstract: pIP501 and pAM beta 1 are promiscuous plasmids from gram-positive bacteria which carry structurally and genetically related replication functions (L. Jannière, A. Gruss and S.D. Ehrlich, 1993, in "Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics (A.L. Sonenshein, J.A. Hoch, and R. Losick, Eds.), pp. 625-644). We report here the sequence of a approximately 1 kb region located downstream of the pIP501 minimal replicon. This region is 82% homologous to the corresponding region of pAM beta 1 and encodes a resolution system which complements that of pAM beta 1. Additionally, we present evidence that the structural similarity between the two plasmids extends for further approximately 2 kb, through a region which may encode a topoisomerase protein in pAM beta 1.
authors.affiliation: Laboratoire de Génétique Microbienne, Institut des Biotechnologies, INRA-Domaine de Vilvert, Jouy en Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Pujol
authors.forename: S D
authors.initials: SD
authors.lastname: Ehrlich
authors.forename: L
authors.initials: L
authors.lastname: Jannière
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D004261:DNA Replication; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006094:Gram-Positive Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C087014:Res beta protein, Enterococcus faecalis; C087015:resIP protein, Streptococcus agalactiae