title: Characterization of a Mycoplasma hominis gene encoding lysyl-tRNA synthetase (LysRS).
abstract: The gene encoding lysyl-tRNA synthetase (lysS) in Mycoplasma hominis was cloned and sequenced. The gene was found to have an open reading frame of 1466 bp encoding a polypeptide with a predicted molecular mass of 57 kDa. The amino acid sequence showed 44.3% and 43.7% identity to the Escherichia coli lysyl-tRNA synthetases, encoded by lysS and lysU. Only one lysyl-tRNA synthetase encoding gene was found in M. hominis. The G + C content of the gene was found to be 28.6%, which is significantly lower than in other prokaryotes. The gene was located 4 kb upstream of the M. hominis PG21 rRNA B operon.
authors.affiliation: Institute of Medical Microbiology, University of Aarhus, Denmark.
authors.forename: D
authors.initials: D
authors.lastname: Ozkökmen
authors.forename: S
authors.initials: S
authors.lastname: Birkelund
authors.forename: G
authors.initials: G
authors.lastname: Christiansen
mesh_terms: D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008250:Lysine-tRNA Ligase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009174:Mycoplasma / Q000201:enzymology* / Q000235:genetics*; D016366:Open Reading Frames; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D008250:Lysine-tRNA Ligase