One-megabase yeast artificial chromosome and 400-kilobase cosmid-phage contigs containing the von Hippel-Lindau tumor suppressor and Ca(2+)-transporting adenosine triphosphatase isoform 2 genes.
We have isolated and ordered yeast artificial chromosomes (YACs) and cosmids surrounding the von Hippel-Lindau (VHL) tumor suppressor and plasma membrane Ca(2+)-transporting ATPase isoform 2 (PMCA-2) genes on chromosome 3p25-26. The YAC contig consists of six YACs and covers a region of 1 megabase. A cosmid-phage contig around VHL and PMCA-2 genes (400 kilobases) was established and integrated into the YAC map. Using these clones, we generated an EcoRI map of the 400-kilobase region. PMCA-2 and VHL complementary DNA were positioned entirely within the cosmid-phage contig as well as two polymorphic markers (D3S601 and D3S1317). This physical map of the cloned region will allow a detailed analysis of both the PMCA-2 and VHL genes. Some of the genomic clones may be useful for isolation of the full-length VHL complementary DNA.
Kuzmin
I
I
Laboratory of Immunobiology, Research and Development Center, Maryland 21702-1201.
Stackhouse
T
T
Latif
F
F
Duh
F M
FM
Geil
L
L
Gnarra
J
J
Yao
M
M
Orcutt
M L
ML
Li
H
H
Tory
K
K
D001483:Base Sequence; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000737:chemistry; D002893:Chromosomes, Human, Pair 3; D003360:Cosmids / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D006623:von Hippel-Lindau Disease / Q000235:genetics*
D018076:DNA, Complementary; D000252:Calcium-Transporting ATPases