Blood/air partition coefficient determinations: automation and improvement of the equilibrium partitioning in closed systems method.
The equilibrium partitioning in closed systems method has been frequently used for determining partition coefficients between liquid and gas phases. We developed several improvements in this method: preparation of test atmospheres with a dynamic solvent vapor generator, automation of gas phase analysis, and estimation of the precision of the partition coefficients using a statistical procedure that did not require pairing of test and reference measurements. Blood/air partition coefficients measured in a blood-bank blood sample for acetone, 1,1,1-trichloroethane, toluene, and styrene were: 309 +/- 2, 5.6 +/- 0.1, 16.2 +/- 0.5 and 49 +/- 2 (geometric mean +/- estimated SE, respectively). Water/air partition coefficients were: 361 +/- 7, 2.9 +/- 0.3, 4.6 +/- 0.5, and 6.9 +/- 0.9 for acetone, 1,1,1-trichloroethane, toluene, and styrene.
Dills
R L
RL
Department of Environmental Health, School of Public Health and Community Medicine, University of Washington, Seattle 98195.
Ackerlund
W S
WS
Kalman
D A
DA
Morgan
M S
MS
D000096:Acetone / Q000493:pharmacokinetics; D001322:Autoanalysis / Q000379:methods*; D015824:Blood-Air Barrier / Q000502:physiology*; D006801:Humans; D020058:Styrene; D013343:Styrenes / Q000493:pharmacokinetics; D014050:Toluene / Q000493:pharmacokinetics; D014240:Trichloroethanes / Q000493:pharmacokinetics
D013343:Styrenes; D014240:Trichloroethanes; C024566:1,1,1-trichloroethane; D000096:Acetone; D014050:Toluene; D020058:Styrene