[Chemical reactions in double-helical nucleic acids. XVI. Synthesis of DNA-duplexes containing regularly repeating transverse covalent cross- links].
Two self-complementary decadeoxyribonucleotides TAATGC*ATTA (where C* is a derivative of 5-methyl cytosine with a carboxy- or aminofunction attached through a spacer to the exocyclic amino group) were synthesized. Carbodiimide induced condensation of the amino and carboxyl groups in the opposite strands to give the crosslinks with a yield up to 20%. Cross-linking of two opposite strands in the duplex formed by the self-complementary aliphatic amino group-containing decanucleotide was performed with the use of glutaric aldehyde with a similar efficiency. The structure of the dimers obtained and position of the crosslinks were confirmed by the Maxam--Gilbert method. Efficiencies of the T4 DNA ligase-induced polycondensations of the double-stranded modified decanucleotides and of the cross-linked products differed significantly.
Koroleva
O N
ON
Volkov
E M
EM
Oretskaia
T S
TS
Shabarova
Z A
ZA
D001483:Base Sequence; D003432:Cross-Linking Reagents; D011088:DNA Ligases; D018076:DNA, Complementary / Q000737:chemistry*; D008969:Molecular Sequence Data
D003432:Cross-Linking Reagents; D018076:DNA, Complementary; D011088:DNA Ligases
Khimicheskie reaktsii v dvuspiral'nykh nukleinovykh kislotakh. XVI. Sintez DNK-dupleksov, soderzhashchikh reguliarno povtoriaiushchiesia poperechnye kovalentnye sshivki.