Determination of ketone bodies in postmortem blood by head-space gas chromatography.
A method describing determination of the physiological ketone bodies: acetone, acetoacetate, and D-beta-hydroxybutyrate in postmortem blood by head-space gas chromatography. The method involves enzymic dehydrogenation of D-beta-hydroxybutyrate into acetoacetate and quantitative conversion of acetoacetate into acetone. The recoveries of acetone, acetoacetate and D-beta-hydroxybutyrate were 94-100%, 88-104% and 83-91%, respectively.
Felby
S
S
Institute of Forsenic Chemistry, University of Copenhagen, Denmark.
Nielsen
E
E
D002849:Chromatography, Gas / Q000379:methods*; D003653:Decarboxylation; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007657:Ketone Bodies / Q000097:blood*; D011180:Postmortem Changes; D013696:Temperature
D007657:Ketone Bodies