title: KSAYMRFamide: a novel FMRFamide-related heptapeptide from the free-living nematode, Panagrellus redivivus, which is myoactive in the parasitic nematode, Ascaris suum.
abstract: In nematodes, FMRFamide-related peptides (FaRPs) have been structurally characterised from the parasite, Ascaris suum, and from two free-living species, Panagrellus redivivus and Caenorhabditis elegans. While both FaRPs isolated from P. redivivus (PF1 and PF2) have been identified in C. elegans, the two heptapeptides isolated from A. suum (AF1 and AF2) have until recently been considered unique to this parasitic species. We have recently isolated AF2 from P. redivivus and, during this study, an additional novel heptapeptide amide, Lys-Ser-Ala-Tyr-Met-Arg-Phe amide (KSAYMRFamide), was structurally characterised. A synthetic replicate of this peptide induced a rapid concentration-dependent muscle tension increase in an isolated. A. suum somatic muscle preparation, with a threshold of approximately 0.1 microM. These data suggest that the complement of FaRPs in parasitic and free-living nematodes may not be as radically different as preliminary studies would suggest, and that the absence of AF1, AF2 and KSAYMRFamide on the C.elegans FMRFamide-related peptide gene (flp-1) may imply the presence of at least two different FaRP genes in nematodes.
authors.affiliation: Comparative Neuroendocrinology Research Group, School of Clinical Medicine, Queen's University of Belfast, Northern Ireland.
authors.forename: A G
authors.initials: AG
authors.lastname: Maule
authors.forename: C
authors.initials: C
authors.lastname: Shaw
authors.forename: J W
authors.initials: JW
authors.lastname: Bowman
authors.forename: D W
authors.initials: DW
authors.lastname: Halton
authors.forename: D P
authors.initials: DP
authors.lastname: Thompson
authors.forename: T G
authors.initials: TG
authors.lastname: Geary
authors.forename: L
authors.initials: L
authors.lastname: Thim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017165:Ascaris suum / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D019835:FMRFamide; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009479:Neuropeptides / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D017168:Rhabditida / Q000235:genetics / Q000502:physiology*
chemical_list: D015801:Helminth Proteins; C087068:KSAYMRFamide; D009479:Neuropeptides; D019835:FMRFamide