Direct PCR sequencing of the ndd gene of bacteriophage T4: identification of a product involved in bacterial nucleoid disruption.
The rapid disruption of the Escherichia coli nucleoid after T4 infection requires the activity of the phage-encoded ndd gene. We have genetically identified the sequence encoding ndd. Determination of the sequence of a 2.5-kb segment including ndd closed the last significant gap in the sequence of the T4 genome. This analysis was performed on PCR-amplified fragments that were purified by gel-exclusion chromatography and then submitted to linear amplification cycle sequencing. This technology permitted sequence comparison of two ndd mutants (ndd44 and ndd98) with the wild-type gene. The analysis of ndd from six bacteriophages of the T-even family indicated that the protein encoded by this nonessential gene is surprisingly conserved.
Bouet
J Y
JY
Laboratoire de Microbiologie et Génétique Moléculaire, CNRS, Toulouse, France.
Woszczyk
J
J
Repoila
F
F
François
V
V
Louarn
J M
JM
Krisch
H M
HM
D000595:Amino Acid Sequence; D017122:Bacteriophage T4 / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli; D005814:Genes, Viral / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
C086890:Ndd protein, Enterobacteria phage T4; D014764:Viral Proteins