title: DNA deoxyribophosphodiesterase and an activity that cleaves DNA containing thymine glycol adducts in Deinococcus radiodurans.
abstract: Deinococcus radiodurans is the most radioresistant bacterium discovered to date. Recently it has been demonstrated that this organism contains the DNA repair enzyme uracil-DNA glycosylase and an apurinic/apyrimidinic (AP) endonuclease that may function as part of a DNA base excision repair pathway. We demonstrate here that a DNA deoxyribophosphodiesterase activity that acts on incised AP sites in DNA to remove deoxyribose-phosphate groups is found in lysates prepared from D. radiodurans cells. The partially purified activity was found to be smaller in size than the E. coli dRpase activity, with an estimated molecular weight of 25-30 kDa. In addition, an activity that recognizes and cleaves DNA containing thymine glycols was also detected, with a molecular weight of approximately 30 kDa. This enzyme may be analogous to the thymine glycol glycosylase/AP lyase endonuclease III of E. coli.
authors.affiliation: Department of Radiation Oncology, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: C
authors.initials: C
authors.lastname: Mun
authors.forename: J
authors.initials: J
authors.lastname: Del Rowe
authors.forename: M
authors.initials: M
authors.lastname: Sandigursky
authors.forename: K W
authors.initials: KW
authors.lastname: Minton
authors.forename: W A
authors.initials: WA
authors.lastname: Franklin
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006095:Gram-Positive Cocci / Q000201:enzymology* / Q000235:genetics; D006868:Hydrolysis; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013941:Thymine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; C029389:thymine glycol; C058246:DNA deoxyribophosphodiesterase; D010727:Phosphoric Diester Hydrolases; D013941:Thymine