Activation-dependent ubiquitination of a T cell antigen receptor subunit on multiple intracellular lysines.
The T cell antigen receptor zeta chain and other T cell antigen receptor components are ubiquitinated on receptor occupancy. A systematic mutagenesis of the zeta subunit was undertaken to determine the sites of ubiquitination. Ubiquitination was found to occur in the cytoplasmic domain of zeta with multiple lysines serving as sites for mono- and polyubiquitination. The mutation of all potential sites of ubiquitination did not inhibit receptor tyrosine phosphorylation or the ubiquitination of other T cell antigen receptor subunits. Lysines introduced into nonnative positions in the zeta molecule were also able to serve as sites for ubiquitination. These findings demonstrate that once a T cell antigen receptor is targeted for ubiquitination, there is little specificity with regard to the lysine residues that are modified.
Hou
D
D
Laboratory of Immune Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892.
Cenciarelli
C
C
Jensen
J P
JP
Nguygen
H B
HB
Weissman
A M
AM
D000595:Amino Acid Sequence; D000818:Animals; D010856:Columbidae; D015180:Electrophoresis, Gel, Two-Dimensional; D008239:Lysine / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000737:chemistry / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism*
D016692:Receptors, Antigen, T-Cell, gamma-delta; D014452:Ubiquitins; D014443:Tyrosine; D008239:Lysine