title: A new tool for investigating G protein-coupled receptors.
abstract: Vertebrate olfactory receptors are members of the seven-transmembrane-domain G protein-coupled receptor family. They utilize intracellular signal transduction pathways which are activated by stimulation of odorant receptors and use the second messengers cAMP and/or inositol 1,4,5-trisphosphate and diacylglycerol. Studies of how odorants bind to and activate the receptors can be considered part of the more general problem of how chemicals interact with G protein-coupled receptors. This review describes the development of a new technique for assessing functional interactions between chemicals and these receptors in only minutes. Predicted uses of the system include structure-function analyses of both G protein-coupled receptors and their ligands, studies of receptor coupling to G proteins and cloning of cDNAs for these receptors.
authors.affiliation: Howard Hughes Medical Institute Research Laboratories, Yale University School of Medicine, New Haven, CT 06536-0812.
authors.forename: M R
authors.initials: MR
authors.lastname: Lerner
authors.forename: M N
authors.initials: MN
authors.lastname: Potenza
authors.forename: G F
authors.initials: GF
authors.lastname: Graminski
authors.forename: T
authors.initials: T
authors.lastname: McClintock
authors.forename: C K
authors.initials: CK
authors.lastname: Jayawickreme
authors.forename: S
authors.initials: S
authors.lastname: Karne
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000378:metabolism*; D018035:Receptors, Odorant / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D018035:Receptors, Odorant; D011994:Recombinant Proteins; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases