Molecular cloning, functional expression, and chromosomal localization of the human cholecystokinin type A receptor.
The results presented here describe for the first time the molecular cloning of the human CCKA-R. Expression of the recombinant receptor shows the expected subtype pharmacology and coupling to phosphoinositide hydrolysis reported for the native human CCKA-R. This knowledge will enhance our understanding of its distribution, pharmacology, and structure and will improve our understanding of its physiological role in the gastrointestinal and nervous systems in humans. Ultimately, this should hasten the understanding and therapy of gastrointestinal and neuropsychiatric disorders.
Pisegna
J R
JR
Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
de Weerth
A
A
Huppi
K
K
Wank
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods; D004064:Digestive System / Q000378:metabolism; D005704:Gallbladder / Q000378:metabolism; D015870:Gene Expression; D015698:Genomic Library; D006168:Guinea Pigs; D006801:Humans; D008969:Molecular Sequence Data; D009420:Nervous System / Q000378:metabolism; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D051381:Rats; D011949:Receptors, Cholecystokinin / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D011949:Receptors, Cholecystokinin