Sequences of the oxytetracycline polyketide synthase-encoding otc genes from Streptomyces rimosus.
The complete nucleotide sequences of the Streptomyces rimosus oxytetracycline (oxyTc) polyketide synthase (PKS)-encoding genes (otcY) has been determined, revealing three open reading frames. The deduced amino-acid sequences correspond to the presumed heterodimeric beta-ketoacyl synthase and acyl carrier protein found in other type-II (multicomponent) PKS systems that specify construction of acetate-derived polyketide antibiotics.
Kim
E S
ES
Department of Microbiology, University of Minnesota, St. Paul 55108.
Bibb
M J
MJ
Butler
M J
MJ
Hopwood
D A
DA
Sherman
D H
DH
D000595:Amino Acid Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics*; D010118:Oxytetracycline / Q000096:biosynthesis*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D009097:Multienzyme Complexes; D010118:Oxytetracycline