Involvement of immunologic mechanisms in a guinea pig model of western red cedar asthma.
Western red cedar asthma is the most common form of occupational asthma in the Pacific Northwest. Plicatic acid (PA) is the chemical component of Western red cedar that causes asthma. The role of immunologic processes involved in the PA-induced asthmatic reaction has not been established. To characterize the mechanisms of PA-induced asthmatic reaction, guinea pigs were sensitized to PA through biweekly injection of PA-ovalbumin conjugate with aluminum hydroxide as an adjuvant for a period of 6 months. Specific IgG1 antibodies to PA were detected in the blood 3 months after sensitization of animals. The level of specific IgG1 antibodies to ovalbumin after 6 months was about two times the level of specific IgG1 to PA. At 6 months, tracheal tissue from PA-ovalbumin-sensitized guinea pigs contracted after exposure to either PA or ovalbumin in vitro. The degree of contraction induced by PA was two to three times less than the contraction induced by ovalbumin. PA caused histamine, prostaglandin D2, and leukotriene D4 release from both lung mast cells and blood basophils. The amount of histamine and eicosanoids released by PA was also two to three times less than the amount of mediators released by ovalbumin. When the trachea of normal guinea pigs was passively sensitized with serum from PA-ovalbumin-sensitized guinea pigs, it contracted in response to PA or ovalbumin in an organ bath. When the serum of PA-ovalbumin-sensitized guinea pigs was depleted of immunoglobulins and then used for passive sensitization of normal trachea, no contraction was observed when challenged with PA, suggesting that IgG1 antibodies mediate the tracheal reaction to PA.(ABSTRACT TRUNCATED AT 250 WORDS)
Salari
H
H
Department of Medicine, University of British Columbia, Vancouver, Canada.
Howard
S
S
Chan
H
H
Dryden
P
P
Chan-Yeung
M
M
D000485:Allergens / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D001249:Asthma / Q000209:etiology / Q000276:immunology*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D017998:Leukotriene D4 / Q000032:analysis; D017705:Lignans; D008168:Lung / Q000187:drug effects / Q000276:immunology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000276:immunology; D009130:Muscle, Smooth / Q000187:drug effects / Q000276:immunology; D009284:Naphthols / Q000276:immunology / Q000494:pharmacology; D015230:Prostaglandin D2 / Q000032:analysis; D013997:Time Factors; D014132:Trachea / Q000187:drug effects / Q000276:immunology; D014197:Trees / Q000276:immunology*
D000485:Allergens; D007074:Immunoglobulin G; D017705:Lignans; D009284:Naphthols; D017998:Leukotriene D4; C008938:plicatic acid; D015230:Prostaglandin D2