[Cloning and expression of certain herpes simplex virus type 1 genes in Escherichia coli].
Complete genes IE12, IE63, IE68, IE175, UL19, UL29 of herpes simplex virus type I or their fragments have been cloned in Escherichia coli cells. The peptides expressed were shown to be fused with cro-beta-galactosidase proteins. The recombinant proteins containing amino acid sequences of ICP4, ICP27 and ICP47, major capsid protein, and major DNA-binding protein react in immunoblotting with the anti-HSVI serum from hyperimmune rabbit. The recombinant proteins can be used for creation of diagnosticums and other scientific and practical purposes. The immunological properties of the recombinant proteins are being investigated.
Shatalin
K Iu
KIu
Zvonarev
A Iu
AIu
Klichko
V I
VI
Karaseva
E V
EV
Kuliakina
M N
MN
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D007106:Immune Sera; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010455:Peptides / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D018139:Simplexvirus / Q000235:genetics*
D007106:Immune Sera; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011994:Recombinant Proteins
Klonirovanie i ékspressiia v kletkakh Escherichia coli nekotorykh genov virusa gerpesa prostogo tipa 1.