Detection of carboxyhemoglobin using Fourier transform infrared microspectroscopy.
We investigated the absorption of carboxyhemoglobin (COHb) using a Fourier transform infrared (FTIR) microscopy system. Weak absorption at 1969 cm-1 due to Fe<--CO and strong absorption due to N-H stretching vibration, > C = O stretching vibration and -NH bending vibration (1544 cm-1) were observed. The ratio of absorbance at 1969 cm-1/1544 cm-1 can be used as an index of CO saturation. Therefore, when formalin-fixed blood remains in blood vessels in formalin-fixed tissues, the amount of COHb can be calculated using this FTIR microscopy system.
Mori
A
A
Department of Legal Medicine, School of Medicine, Teikyo University.
Shioda
T
T
Takeda
E
E
Genma
M
M
Suzuki
H
H
Ishiyama
I
I
D000818:Animals; D002263:Carboxyhemoglobin / Q000032:analysis*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013055:Spectrophotometry, Infrared
D002263:Carboxyhemoglobin