title: Basal metallothionein in tumors: widespread presence of apoprotein.
abstract: A survey has been conducted of solid and ascites tumors from mice and solid tumors in rats for the presence of metallothionein or metallothionein-like protein. In most tumors, a positive identification was made on the basis of Sephadex G-75 and HPLC-DEAE chromatography followed by competitive radioimmunoassay for metallothionein. Apometallothionein was revealed in a number of tumors for the first time by comparing the Sephadex G-75 chromatographic profiles of Zn in native cytosol and Cd in cytosol incubated briefly with CdCl2 to saturate free binding sites on the protein before Sephadex G-75 chromatography. In two cases unsaturation of metallothionein was correlated with a lack of zinc in the ascites fluid which supplies the tumor with zinc.
authors.affiliation: Department of Chemistry, University of Wisconsin-Milwaukee 53201.
authors.forename: A
authors.initials: A
authors.lastname: Pattanaik
authors.forename: C F
authors.initials: CF
authors.lastname: Shaw
authors.forename: D H
authors.initials: DH
authors.lastname: Petering
authors.forename: J
authors.initials: J
authors.lastname: Garvey
authors.forename: A J
authors.initials: AJ
authors.lastname: Kraker
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000032:analysis*; D001667:Binding, Competitive; D002104:Cadmium / Q000032:analysis / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry; D008546:Melanoma, Experimental / Q000737:chemistry; D008668:Metallothionein / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000737:chemistry*; D011863:Radioimmunoassay; D051381:Rats; D012208:Rhabdomyosarcoma / Q000737:chemistry; D012513:Sarcoma, Experimental / Q000737:chemistry; D013160:Splenic Neoplasms / Q000737:chemistry; D015032:Zinc / Q000032:analysis / Q000378:metabolism
chemical_list: D001059:Apoproteins; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc