title: 5-Aminolevulinic acid synthesis in Escherichia coli requires expression of hemA.
abstract: hemA and hemM, which are 213 bp apart and divergently transcribed, were separately cloned. We found that hemA is required for 5-aminolevulinic acid (ALA) synthesis in two ALA- auxotrophs. Overexpression of hemM alone did not produce ALA. More ALA was produced by strains harboring a plasmid with both hemA and hemM than by those with hemA alone. We conclude that hemA alone is required for ALA synthesis but hemA and hemM are required for maximal ALA synthesis.
authors.affiliation: Department of Biochemistry, City College of City University of New York, New York 10031.
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: C S
authors.initials: CS
authors.lastname: Russell
authors.forename: Y
authors.initials: Y
authors.lastname: Murooka
authors.forename: S D
authors.initials: SD
authors.lastname: Cosloy
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000622:Aminolevulinic Acid / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C087012:LolB protein, E coli; D018832:Molecular Chaperones; D000622:Aminolevulinic Acid; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase