title: ANP binding sites are increased in choroid plexus of SLS-1 rats after 9 days of spaceflight.
abstract: Specific alpha-rat 28-amino acid atrial natriuretic peptide [ANP(99-126)] (rANP) binding sites in choroid plexus and meningia of rats flown for 9 days on the mission STS-40 (SLS-1) carried on the space shuttle Columbia in June 1991 were analyzed after incubation of brain sections with 125I-rANP and autoradiography, using computer-assisted microdensitometric image analysis. The number of 125I-rANP binding sites (expressed by Bmax values) in the choroid plexus of lateral and third ventricles of these rats was significantly increased (x 1.5-2.5 times), as compared with that found in ground control rats. No differences in the binding affinity (deducible from Kd values) were observed at the level of these structures. The choroid plexus from the fourth ventricle of the same rats displayed no changes in the binding capacity or affinity after the spaceflight. Meningia from the rats flown in space did not demonstrate any significant modifications of the number of 125I-rANP binding sites, but displayed a significant increase in Kd values, which suggested a reduced affinity of the meningeal ANP receptors during a 9-d spaceflight. The possibility that atrial natriuretic peptide may be involved in the regulation of fluid and electrolyte fluxes in the brain, during adaptation to microgravity, through modified expression of specific high affinity receptors, mainly in choroid plexus from forebrain or in meningia, must be considered.
authors.affiliation: URA CNRS 530, Université de Montpellier II, France.
authors.forename: S
authors.initials: S
authors.lastname: Herbuté
authors.forename: J
authors.initials: J
authors.lastname: Oliver
authors.forename: J
authors.initials: J
authors.lastname: Davet
authors.forename: M
authors.initials: M
authors.lastname: Viso
authors.forename: R W
authors.initials: RW
authors.lastname: Ballard
authors.forename: C
authors.initials: C
authors.lastname: Gharib
authors.forename: J
authors.initials: J
authors.lastname: Gabrion
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001345:Autoradiography; D002831:Choroid Plexus / Q000378:metabolism*; D008578:Meninges / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013026:Space Flight
chemical_list: D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor