title: Recognition of UUN codons by two leucine tRNA species from Escherichia coli.
abstract: Codon recognition by Escherichia coli tRNA(Leu)4 and tRNA(Leu)5 was investigated by analysis of the competition between two aminoacyl-tRNA species in an in vitro protein synthesis. Both tRNA species strictly obey the wobble rule when they are in competition with other tRNA species. This is probably due to the post-transcriptional modifications at the first position of the anticodon of these tRNA(Leu) species, supporting the proposal that the conformational rigidity of post-transcriptionally modified pyrimidine nucleotides guarantees the correct codon recognition.
authors.affiliation: Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takai
authors.forename: N
authors.initials: N
authors.lastname: Horie
authors.forename: Z
authors.initials: Z
authors.lastname: Yamaizumi
authors.forename: S
authors.initials: S
authors.lastname: Nishimura
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000595:Amino Acid Sequence; D000926:Anticodon; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D015500:Chloramphenicol O-Acetyltransferase / Q000737:chemistry / Q000235:genetics; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010649:Phenylalanine / Q000235:genetics; D012356:RNA, Transfer, Leu / Q000378:metabolism*
chemical_list: D000926:Anticodon; D003062:Codon; D012356:RNA, Transfer, Leu; D010649:Phenylalanine; D015500:Chloramphenicol O-Acetyltransferase