title: Intracellular dynamics of ricin followed by fluorescence microscopy on living cells reveals a rapid accumulation of the dimeric toxin in the Golgi apparatus.
abstract: The intracellular dynamics of fluorescent conjugates of the toxic lectin ricin was followed by video fluorescence microscopy on living CHO cells, demonstrating that the ricin heterodimer and its isolated B chain, after binding to the plasma membrane receptors, migrate to and accumulate in the Golgi apparatus following internalization. A ricin derivative labelled with fluorescein on the A chain and rhodamine on the B chain did not display significant splitting of the A-B heterodimer during translocation of the toxin to the Golgi; this novel finding provides support for the hypothesis that further processing of ricin takes place in this cellular compartment.
authors.affiliation: Dipartimento di Scienze Biochimiche, C.N.R., Università di Roma La Sapienza, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Lendaro
authors.forename: R
authors.initials: R
authors.lastname: Ippoliti
authors.forename: A
authors.initials: A
authors.lastname: Bellelli
authors.forename: M
authors.initials: M
authors.lastname: Brunori
authors.forename: V
authors.initials: V
authors.lastname: Evangelista
authors.forename: D
authors.initials: D
authors.lastname: Guidarini
authors.forename: P A
authors.initials: PA
authors.lastname: Benedetti
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D019793:Fluorescein; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006056:Golgi Apparatus / Q000378:metabolism*; D046911:Macromolecular Substances; D008856:Microscopy, Fluorescence; D012235:Rhodamines; D012276:Ricin / Q000378:metabolism*; D014741:Video Recording
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D012235:Rhodamines; C009434:tetramethylrhodamine isothiocyanate; D012276:Ricin; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein