Characterisation of sin, a potential recombinase-encoding gene from Staphylococcus aureus.
The staphylococcal beta-lactamase (Bla) transposon Tn4002 has previously been reported to have a high level of insertional specificity for a 1.8-kb region on the plasmid pSK1. Nucleotide sequences of this region and of a related region on plasmid pI9789 were determined. Sequence analysis revealed that these two plasmids contain sin, a gene whose deduced product shows similarity to a family of DNA recombinases. Southern hybridisation analysis indicated that sin is located on alpha-, beta- and gamma-families of Bla plasmids and on pSK1 family plasmids. A region of dyad symmetry located upstream from sin on pSK1 and pI9789 was identified as the site of insertions of Tn552 and Tn4002 in separate isolates.
Paulsen
I T
IT
School of Biological Sciences, University of Sydney, NSW, Australia.
Gillespie
M T
MT
Littlejohn
T G
TG
Hanvivatvong
O
O
Rowland
S J
SJ
Dyke
K G
KG
Skurray
R A
RA
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004254:DNA Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D019426:Integrases; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D045522:Recombinases; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1; C086893:sin protein, Staphylococcus aureus