title: [Complementary-addressed photomodification of DNA-targets by arylazide and perfluoroarylazide oligonucleotide derivatives. IV. Photomodification of ss- and ds-DNA-fragments].
abstract: A highly efficient sequence-specific photomodification of single stranded (ss) and double stranded (ds) DNA fragments was carried out with hexadecathymidilate derivative, R-p(T)16(R--p-azidotetrafluorobenzamide) and 27-meric DNA fragments as a targets. [formula: see text] The main points of the modification were G7 and G24 for the ss target and G7 and G22 of purine- and pyrimidine-rich strands, respectively, for the ds DNA fragment. The photomodification extent was 60-77% for ss DNA and 10-53% for ds DNA depending on the reaction conditions: it increased in a buffer with a high ionic strength (1.0 M) and at a low temperature (4 degrees C) when the triplexes are more stable.
authors.forename: A S
authors.initials: AS
authors.lastname: Levina
authors.forename: D R
authors.initials: DR
authors.lastname: Tabatadze
authors.forename: V F
authors.initials: VF
authors.lastname: Zarytova
authors.forename: M I
authors.initials: MI
authors.lastname: Dobrikov
authors.forename: V V
authors.initials: VV
authors.lastname: Gorn
authors.forename: S G
authors.initials: SG
authors.lastname: Lokhov
mesh_terms: D001386:Azides / Q000737:chemistry*; D001483:Base Sequence; D003080:Cold Temperature; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D009994:Osmolar Concentration; D010777:Photochemistry
chemical_list: D001386:Azides; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D004247:DNA
vernacular_title: Komplementarno-adresovannaia fotomodifikatsiia DNK-misheneĭ arilazidnymi i perftorarilazidnymi proizvodnymi oligonukleotidov. IV. Fotomodifikatsiia ss- i ds-DNK-fragmentov.