title: Dietary fat regulates cellular retinol-binding protein II gene expression in rat jejunum.
abstract: Cellular retinol-binding protein II (CRBP II) is an abundant cytosolic protein of intestinal absorptive cells. In this study, we examined whether dietary fat modulates the expression of CRBP II in the small intestine. In the rats fed a diet rich in long-chain triacylglycerols (LCT), both CRBP II mRNA and CRBP II protein levels in the jejunum were more than two-fold greater than in the rats fed a low fat diet and a diet rich in medium-chain triacylglycerols (MCT). The mRNA abundance of a retinoid X receptor (RXR alpha), which is thought to interact with the cis-element located in the CRBP II promoter, was elevated in the jejunum of rats fed high-LCT and high-MCT diets as compared with that of animals fed a low-fat diet, but the levels of RXR alpha mRNA of the LCT diet group was similar to that of MCT diet group. These results suggest that the expression level of the CRBP II gene is not directly related to the RXR alpha expression, and that it might be modulated by long-chain fatty acids or their metabolites.
authors.affiliation: Department of Nutrition, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Goda
authors.forename: H
authors.initials: H
authors.lastname: Yasutake
authors.forename: S
authors.initials: S
authors.lastname: Takase
mesh_terms: D000818:Animals; D001483:Base Sequence; D004041:Dietary Fats / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012177:Retinol-Binding Proteins / Q000235:genetics*; D054840:Retinol-Binding Proteins, Cellular; D014280:Triglycerides / Q000494:pharmacology*
chemical_list: D004041:Dietary Fats; D012333:RNA, Messenger; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014280:Triglycerides