title: Adhering heat-killed human Lactobacillus acidophilus, strain LB, inhibits the process of pathogenicity of diarrhoeagenic bacteria in cultured human intestinal cells.
abstract: Heat-killed L. acidophilus, strain LB, was tested for its ability to adhere in vitro onto human enterocyte-like Caco-2 and muco-secreting HT29-MTX cells in culture. The heat-killed LB bacteria exhibited a high adhesive property. A diffuse pattern of adhesion was observed to the undifferentiated cells, the apical brush border of the enterocytic cells, and to the mucus layer that covered the surface of the mucus-secreting cells. The inhibitory effect of heat-killed LB organisms against the human intestinal Caco-2 cell-adhesion and cell-invasion by a large variety of diarrhoeagenic bacteria was investigated. The following dose-dependent inhibitions were obtained: (i) against the cell-association of enterotoxigenic, diffusely-adhering and enteropathogenic Escherichia coli, Listeria monocytogenes, Yersinia pseudotuberculosis, and Salmonella typhimurium; (ii) against the cell-invasion by enteropathogenic Escherichia coli, Yersinia pseudotuberculosis, Listeria monocytogenes and Salmonella typhimurium.
authors.affiliation: Département de Microbiologie et Immunologie, UFR de Sciences Pharmaceutiques Paris XI, Châtenay-Malabry, France.
authors.forename: M H
authors.initials: MH
authors.lastname: Coconnier
authors.forename: M F
authors.initials: MF
authors.lastname: Bernet
authors.forename: G
authors.initials: G
authors.lastname: Chauvière
authors.forename: A L
authors.initials: AL
authors.lastname: Servin
mesh_terms: D000898:Antibiosis; D001422:Bacterial Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D004755:Enterobacteriaceae / Q000502:physiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000502:physiology*; D008855:Microscopy, Electron, Scanning