Fibronectin synthesis bioactivity in human breast milk.
To evaluate the effect of milk feeding on fibronectin in the perinatal period, fibronectin synthesis bioactivity (FnSA) in human breast milk was examined. FnSA was measured by bioassay using the cell-lined fibroblast. For the characterization of the FnSA, the milk samples were chromatographed. In gel chromatography (Sephadex G-100), three fractions of FnSA were detected. Two fractions overlapped with those of immunoreactive epidermal growth factor (ir-EGF). The other was present in the low-molecular-weight portion. In the high-performance liquid chromatography, two of four fractions with FnSA presented ir-EGF. One of two fractions with FnSA in heparin-binding affinity chromatography almost coincided with the portions of basic brain fibroblast growth factor (ir-bFGF). From these findings, we speculated that human milk has the bioactivity of fibronectin synthesis and it is composed of EGF, FGF, and unknown low-molecular-weight factors. Although the clinical meanings of FnSA have not been clarified, our data indicate a new characteristic of human milk.
Fukushima
N
N
Department of Pediatrics, Gunma University School of Medicine, Japan.
Nagashima
K
K
Kuroume
T
T
D001681:Biological Assay; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology; D005353:Fibronectins / Q000096:biosynthesis*; D006801:Humans; D015151:Immunoblotting; D008895:Milk, Human / Q000378:metabolism*
D005353:Fibronectins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor