Hepatocyte aggregate culture technique for bioreactors in hybrid liver support systems.
Utilizing a modified culture technique for hepatocytes, a high performance suspension culture is possible in which hepatocytes spontaneously form cell aggregates. The aggregates of 20-100 cells have been histologically confirmed to hold a three-dimensional structure, they show a long-term external metabolism and a survival time comparable with standard adhesion cultures. This technique has several advantages in the construction of large scale bioreactors for hybrid liver support systems.
Gerlach
J C
JC
Chirurgische Klinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.
Klöppel
K
K
Müller
C
C
Schnoy
N
N
Smith
M D
MD
Neuhaus
P
P
D000818:Animals; D001187:Artificial Organs; D002449:Cell Aggregation; D002470:Cell Survival; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology*; D008297:Male; D008722:Methods; D051381:Rats; D017208:Rats, Wistar