[Research on anti-idiotypic antibodies to bovine abortus].
Based on the establishment of McAbs to a local Brucella strain S85A, the McAb A7 was selected and tested as a protective one. Using A7 to immunize rabbits, we got high titer anti-idiotypic antibodies which were then proved to possess internal image by competitive inhibiting ELISA and catch blocking ELISA. The anti-idiotypic antibodies were then purified and mixed with glycerin to immunize guinea pigs and mice. The results showed that: immunized guinea pigs and mice had produced certain titer antibodies to Brucella. 79.2% of immunized guinea pigs had been protected from the challenge of 544A 4 months later from primary immunization. The number of T ANAE+ in peripheral blood of immunized mice had increased significantly compared to controls. These results suggest that the anti-idiotypic antibodies not only possess the antigen internal image but have the available immunogenicity stimulate animals producing Ab3 and make them get free from the infection.
Gu
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Institute of Veterinary, Xinjiang Academy of Animal Science, Urümqi.
Gu
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Yuan
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Hamit
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002003:Brucella abortus / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization; D051379:Mice; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal