Adrenomedullin stimulates cyclic AMP formation in rat vascular smooth muscle cells.
A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC). The EC50 value of human AM is 2 x 10(-8) M which is slightly higher than that of CGRP (8.5 x 10(-9) M). In a receptor binding assay for AM in rat VSMC, the binding of [125I] AM was competitively inhibited by human AM, but not by human CGRP. Thus, AM is thought to increase intracellular cAMP in rat VSMC via its specific receptor to evoke vasodilation.
Ishizaka
Y
Y
First Department of Internal Medicine, Miyazaki Medical College.
Ishizaka
Y
Y
Tanaka
M
M
Kitamura
K
K
Kangawa
K
K
Minamino
N
N
Matsuo
H
H
Eto
T
T
D053607:Adrenomedullin; D000704:Analysis of Variance; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY
D000959:Antihypertensive Agents; D010455:Peptides; D053607:Adrenomedullin; D000242:Cyclic AMP; D015740:Calcitonin Gene-Related Peptide