Rapid colorimetric bioassay for screening of Fusarium mycotoxins.
The cytotoxicity of Fusarium mycotoxins was evaluated using a trichothecene sensitive cell line (BHK-21, baby hamster kidney cells) in combination with the MTT-cleavage test as an end-point measurement. Cells tended to be more sensitive to the type A trichothecenes with midpoint cytotoxicity values ranging from 1.6 ng/ml for T-2 toxin to 60 ng/ml for scirpentriol. The cytotoxicity value for deoxynivalenol (type B) was 112 ng/ml. The inherent disadvantage of the MTT-assay (formation of insoluble formazan) was overcome by using the analog MTS and measuring the water-soluble formazan directly in the culture media. The MTS-midpoint cytotoxicity values for T-2 toxin and deoxynivalenol (2.1 and 141 ng/ml, respectively), although slightly higher, showed a good correspondence to the MTT-test. Both the MTT- and MTS-cleavage tests are useful for evaluating the cytotoxicity of Fusarium mycotoxins. The replacement of MTT by MTS substantially reduced the number of sample processing steps and the length of time required to complete the cytotoxicity assay.
Rotter
B A
BA
Centre for Food and Animal Research, Agriculture, Canada, Ottawa, Ontario.
Thompson
B K
BK
Clarkin
S
S
Owen
T C
TC
D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D003124:Colorimetry; D006224:Cricetinae; D005670:Fusarium / Q000472:pathogenicity*; D009183:Mycotoxins / Q000032:analysis*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014255:Trichothecenes / Q000032:analysis
D009183:Mycotoxins; D013778:Tetrazolium Salts; D013844:Thiazoles; D014255:Trichothecenes; C022616:thiazolyl blue