Alteration of asparagine-linked glycosylation in serum transferrin of patients with hepatocellular carcinoma.
The asparagine-linked sugar chains in serum transferrin purified from patients with hepatocellular carcinoma (n = 13), healthy individuals (n = 5) and patients with liver cirrhosis (n = 6) were compared. Sugar chains released with N-glycanase from desialylated and pepsin-digested transferrin were derivatized by reductive pyridylamination. Analysis of the sugar chains by high performance liquid chromatography in combination with exoglycosidase digestion revealed an increase of a biantennary complex-type sugar chain with a fucosylated trimannosyl core; Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3) Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc in 7 of 13 cancer patients and an increase of a sugar chain with a fucosylated trimannosyl core and bisecting N-acetylglucosamine; Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(GlcNAc beta 1-4) (Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc in one of the 13 cancer patients. Further, the fucosylated alteration of the sugar chain was detected also in alpha 1-antitrypsin, hemopexin, alpha 1-acid glycoprotein and alpha 2-HS glycoprotein from one of the patients with increased fucosylated transferrin.
Matsumoto
K
K
School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Maeda
Y
Y
Kato
S
S
Yuki
H
H
D001216:Asparagine / Q000378:metabolism*; D014408:Biomarkers, Tumor; D002240:Carbohydrate Sequence; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D008103:Liver Cirrhosis / Q000097:blood; D008113:Liver Neoplasms / Q000097:blood*; D008969:Molecular Sequence Data; D009844:Oligosaccharides; D014168:Transferrin / Q000378:metabolism*
D014408:Biomarkers, Tumor; D006023:Glycoproteins; D009844:Oligosaccharides; D014168:Transferrin; D001216:Asparagine; D006026:Glycoside Hydrolases; D005947:Glucose