Guidelines for measurement of cutaneous blood flow by laser Doppler flowmetry. A report from the Standardization Group of the European Society of Contact Dermatitis.
The report reviews individual-related variables (age, sex, race, anatomical site), intra- and inter-individual variation (temporal, physical and mental activity, food and drugs), and environment-related variables (air convection, temperature). Technical variation, instrument validation including a standard reactive hyperemia experiment, and a standard operating procedure are discussed and included in the guidelines.
Bircher
A
A
Department of Dermatological Research, Leo Pharmaceutical Products, Ballerup, Denmark.
de Boer
E M
EM
Agner
T
T
Wahlberg
J E
JE
Serup
J
J
D000293:Adolescent; D000328:Adult; D000392:Air Movements; D002940:Circadian Rhythm; D003071:Cognition / Q000502:physiology; D005260:Female; D005542:Forearm; D006225:Hand; D006801:Humans; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation / Q000379:methods; D008297:Male; D008597:Menstrual Cycle / Q000502:physiology; D008875:Middle Aged; D010600:Pharmacology; D005082:Physical Exertion / Q000502:physiology; D012039:Regional Blood Flow / Q000502:physiology; D015203:Reproducibility of Results; D012867:Skin / Q000098:blood supply*; D013696:Temperature