The effect of basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) on the survival of septal neurons transplanted into the third ventricle in rats.
Effects of short-term pretreatment with basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) on the survival of neurons transplanted into the third ventricle in rats were studied. Septa from 16-day-old rat embryos were pretreated with growth factor containing medium for 30 min and transplanted into the third ventricle of adult rats. Twenty-one days after the operation, the recipient rats were perfused with 4% paraformaldehyde. Paraffin sections of the removed brains were made, and the sections were stained with cresylviolet. The number of neurons in the grafts was counted under a light microscope. Although pretreatment with NGF was not effect, bFGF at the concentration of 1 and 10 micrograms/ml enhanced the survival of transplanted septal neurons. These results suggest that short-term pretreatment with bFGF could increase the efficiency of neuronal transplantation.
Shitaka
Y
Y
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Saito
H
H
D000818:Animals; D016380:Brain Tissue Transplantation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002552:Cerebral Ventricles / Q000166:cytology; D016332:Fetal Tissue Transplantation; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D012688:Septum Pellucidum / Q000166:cytology / Q000637:transplantation*
D009414:Nerve Growth Factors; D016222:Fibroblast Growth Factor 2