title: Growth inhibition of synchronized trigeminal neurinoma cells by nerve growth factor.
abstract: Nerve growth factor (NGF) reduces the development of trigeminal neurinomas in vivo. To characterize the action of NGF on these tumors, clonal cells (476-16) isolated from a rat trigeminal neurinoma cell line were synchronized at early S phase by aphidicolin, a reversible inhibitor of DNA polymerase alpha, and effects of NGF on DNA replication were examined in vitro. While NGF did not inhibit DNA replication in the ongoing S phase, it reduced the level of DNA synthesis in the succeeding S phase without altering its timing and duration. The inhibitory action was elicited by a brief exposure to NGF during progression through the preceding S to G1 phases with decreasing effectiveness in the later stage of G1.
authors.affiliation: Department of Pathology, Michigan State University, East Lansing 48824.
authors.forename: Y
authors.initials: Y
authors.lastname: Marushige
authors.forename: K
authors.initials: K
authors.lastname: Marushige
authors.forename: A
authors.initials: A
authors.lastname: Koestner
mesh_terms: D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D003390:Cranial Nerve Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005038:Ethylnitrosourea; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009442:Neurilemmoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344; D014276:Trigeminal Nerve / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D004273:DNA, Neoplasm; D009414:Nerve Growth Factors; D016590:Aphidicolin; D005038:Ethylnitrosourea