[Uveitogenicity and immunogenicity of interphotoreceptor retinoid-binding protein-derived peptide R16 in different strains of rats].
Interphotoreceptor retinoid-binding protein (IRBP) can induce experimental autoimmune uveoretinitis (EAU) in different rat strains. This is because IRBP has many epitopes, each of which binds to a specific class II molecule. However, there is the possibility that one epitope can bind to different class II molecules. In this study we attempted to examine this postulate, by checking uveitogenicity and immunogenicity of IRBP-derived peptide R16. We used rat strains LEW, WKAH, SDJ, LEJ, and BUF, which have RT1 haplotype l, k, u, i, and b, respectively. Immunization with R16 caused EAU in LEW (100%), WKAH (75%), SDJ (0%), LEJ (75%), and BUF (19%). We also examined the immunogenicity of R16 by lymphocyte proliferative assay in which lymph node cells from rats of each strain immunized with R16 were stimulated with the immunogen peptide. R16 evoked substantial proliferative responses in LEW, WKAH, LEJ, and BUF, but not in SDJ. An inhibition test with anticlass II monoclonal antibodies showed that OX6 (anti-RT1-B) could inhibit the proliferation against R16, but OX17 (anti-RT1-D) could not. These experiments demonstrated that the same peptide induces the disease and immunity in different strains of rats.
Sasamoto
Y
Y
Department of Ophthalmology, Hokkaido University School of Medicine, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D001327:Autoimmune Diseases / Q000209:etiology*; D005136:Eye Proteins / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D012177:Retinol-Binding Proteins / Q000276:immunology*; D013045:Species Specificity; D014605:Uveitis / Q000209:etiology*; D000099214:Retinol-Binding Proteins, Interstitial
D005136:Eye Proteins; D010446:Peptide Fragments; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial; C084432:immunodominant R16 protein, rat