title: A genomic clone of a type I cab gene encoding a light harvesting chlorophyll a/b binding protein of photosystem II identified from lodgepole pine.
abstract: A type I cab gene encoding chlorophyll a and b binding protein from lodgepole pine (Pinus contorta Dougl., var. latifolia (Engelm.)) has been isolated and sequenced. The gene encodes a proposed 40 amino acid transit peptide and a 234 amino acid mature CAB polypeptide. There is no evidence of an intron and examination of the type-specific amino acids confirm this to be a photosystem II type I cab gene. The coding region is highly conserved with a similar gene from Scots pine, the homology extending into the 5' and 3' untranslated regions. Sequence comparison of the 1-kb region upstream of the translation start codon from the lodgepole pine cab gene exhibited little similarity to identified regulatory regions in pea, tobacco, wheat, and Arabidopsis cab genes. The cab gene, which is 62% G + C rich, is surrounded by 70-75% A + T rich regions, which may confer some regulatory feature on its expression.
authors.affiliation: Department of Forest Science, University of Alberta, Edmonton, Canada.
authors.forename: J W
authors.initials: JW
authors.lastname: Barrett
authors.forename: R N
authors.initials: RN
authors.lastname: Beech
authors.forename: B P
authors.initials: BP
authors.lastname: Dancik
authors.forename: C
authors.initials: C
authors.lastname: Strobeck
mesh_terms: D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014197:Trees / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D004247:DNA