title: Constitutive expression of a somatic heat-inducible hsp70 gene during amphibian oogenesis.
abstract: We isolated and characterized a sequence coding for heat-shock protein 70 (HSP70) of the amphibian Pleurodeles waltl. Results from S1 nuclease protection assays led us to conclude that an hsp70 gene, strictly inducible in somatic cells during heat shock, is constitutively active during oogenesis. By quantitative northern and western blot analysis, we showed that both hsp70 mRNA and HSP70-related protein levels increased in oocytes from stage II to stage VI under physiological conditions. Furthermore, by in situ hybridization to the nascent transcripts of lampbrush chromosome loops, we provided evidence for a clear-cut relationship between this increase in hsp70 mRNA and transcriptional activity during the lampbrush stage of oogenesis. These results strongly suggest that hsp70 genes are actively transcribed throughout oogenesis. HSP70-related proteins localized in the cytoplasm of young oocytes are progressively transferred to the nucleus in the course of oogenesis and preferentially accumulated in the nuclei of some stage VI oocytes.
authors.affiliation: Centre de Biologie Cellulaire, CNRS, Ivry-sur-Seine, France.
authors.forename: B
authors.initials: B
authors.lastname: Billoud
authors.forename: M L
authors.initials: ML
authors.lastname: Rodriguez-Martin
authors.forename: L
authors.initials: L
authors.lastname: Berard
authors.forename: N
authors.initials: N
authors.lastname: Moreau
authors.forename: N
authors.initials: N
authors.lastname: Angelier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D015870:Gene Expression / Q000502:physiology; D006360:Heat-Shock Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000235:genetics*; D010999:Pleurodeles / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D006360:Heat-Shock Proteins