Molecular cloning and functional expression of gicerin, a novel cell adhesion molecule that binds to neurite outgrowth factor.
Gicerin is an integral membrane glycoprotein of about 82 kd that is transiently expressed in the developing CNS. Gicerin was first identified as a binding protein for neurite outgrowth factor (NOF), a member of the laminin family of extracellular matrix proteins. By isolating and sequencing a gicerin cDNA, we have found that this protein is a novel member of the immunoglobulin superfamily. The deduced protein (584 amino acids) consists of five immunoglobulin-like loop structures in an extracellular domain, a single transmembrane region, and a short cytoplasmic tail. Cells transfected stably with gicerin cDNA adhered to NOF and aggregated with each other, indicating that gicerin exhibits both heterophilic and homophilic adhesion activities.
Taira
E
E
Department of Pharmacology, Osaka University School of Medicine, Japan.
Takaha
N
N
Taniura
H
H
Kim
C H
CH
Miki
N
N
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D051929:CD146 Antigen; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000378:metabolism*; D014162:Transfection
D030161:Avian Proteins; D051929:CD146 Antigen; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C497903:Mcam protein, mouse; D009414:Nerve Growth Factors; C086935:gicerin protein, Gallus gallus