Antibodies to type II collagen in human serum: storage effects and role of IgM antiidiotype in regulation.
OBJECTIVE
To investigate the observation that IgG reactivity to human denatured type II collagen (denatured CII) is increased in normal human serum stored at 4 degrees C compared to serum stored at -20 degrees C.


METHODS
Reactivity was measured by ELISA, and the effect of heating was also assessed. Serum was separated on affinity columns and mixing experiments were performed with the resultant fractions to test for inhibitory or enhancing effects.


RESULTS
There is a storage labile and heat labile inhibitor in the IgG depleted fraction of serum stored at -20 degrees C from healthy subjects, and an inhibitor is present in some pooled human IgM preparations which reduces the reactivity of antibody to human CII in serum from both healthy subjects and patients with rheumatoid arthritis.


CONCLUSIONS
Storage conditions and heat significantly affect IgG antibodies to denatured CII but not antibodies to other antigens. These effects may be mediated by the presence of an IgM antiidiotype against the IgG antibody to denatured CII.
Woodruff
T
T
Centre for Molecular Biology and Medicine, Monash University, Clayton, Victoria, Australia.
Cook
A
A
Rowley
M
M
Mackay
I
I
D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D001793:Blood Preservation; D003080:Cold Temperature; D003094:Collagen / Q000737:chemistry / Q000276:immunology*; D005615:Freezing; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011489:Protein Denaturation
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003094:Collagen