title: UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus.
abstract: We have analyzed the sequence downstream of rpoN from Acinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus. The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN. These observations confirm that A. calcoaceticus represents the first exception from a conserved genetic context of rpoN observed in several other Gram-negative bacteria.
authors.affiliation: Institut für Mikrobiologie und Biochemie, Friedrich-Alexander Universität Erlangen-Nürnberg, FRG.
authors.forename: S
authors.initials: S
authors.lastname: Ehrt
authors.forename: W
authors.initials: W
authors.lastname: Hillen
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000235:genetics*; D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D001483:Base Sequence; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases; D004352:Drug Resistance, Microbial; D005578:Fosfomycin / Q000494:pharmacology; D015870:Gene Expression; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D051877:RNA Polymerase Sigma 54; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor / Q000235:genetics; D014166:Transferases / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D012808:Sigma Factor; D005578:Fosfomycin; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C022566:UDP-N-acetylglucosamine 1-carboxyvinyltransferase; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54