Characterization of a 3-dehydroquinase gene from Actinobacillus pleuropneumoniae with homology to the eukaryotic genes qa-2 and QUTE.
A gene was cloned from Actinobacillus pleuropneumoniae strain 4074 by complementation of an aroD strain of Escherichia coli. The E. coli gene aroD codes for a 3-dehydroquinase enzyme of type I, active in the aromatic biosynthesis pathway. The A. pleuropneumoniae gene, termed aroQ, displays no base or amino acid sequence homology to aroD of E. coli. It is instead homologous to the QUTE and qa-2 genes, respectively of Aspergillus nidulans and Neurospora crassa. These genes code for 3-dehydroquinase enzymes of type II, involved in the catabolism of quinic acid. The 1.8 kb fragment, which includes aroQ, carries four overlapping or adjacent open reading frames: a dapD gene; aroQ; one without homology to sequences in GenBank; and one with homology to the C-terminal 40% of chIN of E. coli.
Lalonde
G
G
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
O'Hanley
P D
PD
Stocker
B A
BA
Denich
K T
KT
D016977:Actinobacillus pleuropneumoniae / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000235:genetics; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012763:Shigella flexneri / Q000201:enzymology / Q000235:genetics; D013045:Species Specificity
D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase