title: Mouse sialoadhesin is not responsible for Candida albicans yeast cell binding to splenic marginal zone macrophages.
abstract: Candida albicans hydrophilic yeast cells specifically adhere to mouse macrophages in the splenic marginal zone and in lymph node subcapsular and medullary sinuses. These macrophages express sialoadhesin that binds erythrocytes, but binding of yeast cells is not mediated by sialoadhesin because (i) erythrocytes did not block yeast cell binding, (ii) yeast cell adherence was unaffected by sialoadhesin-specific monoclonal antibodies, (iii) the purified sialoadhesin did not bind to yeast cells, and (iv) an Fc-sialoadhesin chimera immobilized on plastic did not bind yeast cells. These data give further support for a unique macrophage adhesion system that binds C. albicans.
authors.affiliation: Department of Microbiology, Montana State University, Bozeman 59717.
authors.forename: Y
authors.initials: Y
authors.lastname: Han
authors.forename: S
authors.initials: S
authors.lastname: Kelm
authors.forename: M H
authors.initials: MH
authors.lastname: Riesselman
authors.forename: P R
authors.initials: PR
authors.lastname: Crocker
authors.forename: J E
authors.initials: JE
authors.lastname: Cutler
mesh_terms: D000268:Adhesiveness; D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity*; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D011971:Receptors, Immunologic / Q000502:physiology*; D063267:Sialic Acid Binding Ig-like Lectin 1; D013154:Spleen / Q000166:cytology / Q000382:microbiology
chemical_list: D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C571166:SIGLEC1 protein, human; D063267:Sialic Acid Binding Ig-like Lectin 1; C571167:Siglec1 protein, mouse