An improved HPLC method to purify erythrocyte cholesterol for estimation of in vivo cholesterol synthesis using the deuterium method.
We report on an improved HPLC method to purify erythrocyte cholesterol for use in measuring in vivo cholesterol synthesis from 2H2O. The new procedure uses a smaller compression cartridge column at a flow rate of 3 mL/min. The new method reduces by almost tenfold the amount of solvent required to maintain the analytical purity of the isolated cholesterol.
Wong
W W
WW
Department of Pediatrics, Baylor College of Medicine, Houston, TX.
Hachey
D L
DL
Clarke
L L
LL
Zhang
S
S
Llaurador
M
M
Pond
W G
WG
D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D013552:Swine
D002784:Cholesterol; D003903:Deuterium