[Detection of gamma-interferon mRNA in JEG-3 choriocarcinoma cell line by means of polymerase chain reaction].
To investigate the pathogenesis of choriocarcinoma the authors employed a newly developed gene amplification method by reverse polymerase chain reaction for the detection of gamma-interferon messenger RNA in JEG-3 choriocarcinoma cell line. Polymerase chain reaction products were analysed by agarose gel electrophoresis. Using 1 Kb DNA ladder as a marker, 84 base-pair fragment was selectively amplified correlating with published gamma-interferon gene fragment length. Because cDNA contains a virtually complete copy of the mRNA this method provides an evidence for the expression of gamma-interferon gene in JEG-3 choriocarcinoma cell line. Based on these results a potential autocrine mechanism may be present in JEG-3 choriocarcinoma cell line.
Fülöp
V
V
Orvostovábbképzö Egyetem, Budapest, Szülészeti-Nögyógyászati Klinika.
Szigetvári
I
I
Szepesi
J
J
Gáti
I
I
D002822:Choriocarcinoma / Q000737:chemistry / Q000473:pathology*; D005784:Gene Amplification; D005956:Glucose-6-Phosphate Isomerase; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D007371:Interferon-gamma; D005956:Glucose-6-Phosphate Isomerase
A gamma-interferon mRNS kimutatása JEG-3 choriocarcinoma sejtvonalban polimeráz láncreakcióval.