title: A structural study of the asparagine-linked oligosaccharide moiety of duck ovomucoid.
abstract: Asparagine-linked oligosaccharides of duck ovomucoid were released quantitatively from the protein by digestion with glycoamidase A (from almond), the reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-aminopyridine, and the mixture of pyridylamino derivatives of the oligosaccharides was separated using two different types of high performance liquid chromatography (HPLC) on a reversed phase column and an amide adsorption column. More than sixteen different oligosaccharides were separated and the structures were characterized by a combination of the 2-dimensional sugar mapping technique using HPLC, exoglycosidase digestion, and proton nuclear magnetic resonance measurements (1- and 2-dimensional). Furthermore, the HPLC profile of duck ovomucoid oligosaccharides was compared with previously reported profiles obtained from quail and chicken ovomucoids.
authors.affiliation: GlycoLab, Nakano Central Research Institute, Nakano Vinegar Co. Ltd, Handa-City, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Shikami
authors.forename: I
authors.initials: I
authors.lastname: Shimada
authors.forename: Y
authors.initials: Y
authors.lastname: Arata
authors.forename: R
authors.initials: R
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D001216:Asparagine / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002645:Chickens / Q000378:metabolism; D004372:Ducks / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D010059:Ovomucin / Q000737:chemistry* / Q000302:isolation & purification; D011784:Quail / Q000378:metabolism; D013045:Species Specificity
chemical_list: D009844:Oligosaccharides; D010059:Ovomucin; D001216:Asparagine