Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF-immunized mice.
We determined whether Borrelia burgdorferi outer surface proteins (Osps) E and F could elicit immune responses useful for a Lyme disease vaccine. Thirty days after challenge with B. burgdorferi, mice produced antibodies to OspE but not OspF, whereas antibodies to OspF were present in sera of mice obtained 90 days after infection. Examination of sera from patients with Lyme disease revealed antibodies to OspF in a small number (14%) of early-stage disease patients but in a majority (58%) of patients with late-stage disease, while antibodies to OspE were rarely detected in patients. Mice immunized with recombinant OspE or OspF produced high titers of antibodies to OspE or OspF, respectively. OspF-immunized mice were partially protected from both intradermal syringe challenge and tick-mediated transmission of B. burgdorferi while vaccination with OspE did not confer immunity. B. burgdorferi organisms were, however, substantially destroyed within ticks that engorged on either OspE- (75% reduction in the number of spirochetes within the ticks, compared with controls) or OspF (90% reduction in the number of spirochetes within the ticks)-immunized mice.
Nguyen
T P
TP
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520.
Lam
T T
TT
Barthold
S W
SW
Telford
S R
SR
Flavell
R A
RA
Fikrig
E
E
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001426:Bacterial Proteins; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D005260:Female; D006801:Humans; D007114:Immunization; D008074:Lipoproteins; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D011817:Rabbits; D013987:Ticks / Q000382:microbiology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008074:Lipoproteins; C084998:OspE protein, Borrelia burgdorferi; C084999:OspF protein, Borrelia burgdorferi