Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata.
DNA-Dependent RNA polymerase (EC 2.7.7.6) was isolated from Thermomonospora curvata. The purification steps included precipitation with Polymin P, elution of the precipitate with 0.3 mol/L KCl, precipitation with ammonium sulfate, affinity chromatography on heparin-agarose and molecular filtration on Biogel A 1.5 m.
Krausová
M
M
Institute of Microbiology, Academy of Sciences of Czech Republic, Prague.
Klégr
M
M
Spízek
J
J
D000192:Actinomycetales / Q000201:enzymology*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D008970:Molecular Weight; D013171:Spores, Bacterial
D001426:Bacterial Proteins; D012321:DNA-Directed RNA Polymerases