Transcriptional regulation of the hepatic glutaminase gene in the streptozotocin-diabetic rat.
1. Liver possesses a unique isozyme of phosphate activated glutaminase which is subject to long-term regulation. 2. In the rat streptozotocin-diabetes results in a 4-fold increase in the rate of transcription of the rat hepatic glutaminase gene. 3. This is consistent with previous reports from this laboratory of increases, of similar magnitude, in the relative abundance of hepatic glutaminase mRNA (Smith and Watford (1990) J. Biol. Chem. 265, 10631-10636), and enzyme activity (Watford, et al. (1984) Biochem. J. 224, 207-214). 4. The work establishes that, in contrast to the regulation of renal glutaminase where mRNA stability plays an important role, the predominant site of long-term regulation of hepatic glutaminase is at the level of gene transcription.
Zhan
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Department of Nutritional Sciences, Cook College, Rutgers University, New Brunswick, NJ 08903.
Vincent
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NC
Watford
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D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005972:Glutaminase / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
D005972:Glutaminase