Sex identification by polymerase chain reaction using a Y-autosome homologous primer set.
We have developed a one-step polymerase chain reaction (PCR) technique which detects a sequence on the human Y chromosome and an autosomal sequence in one reaction. The method is very reliable for the sex determination, as the detection of the autosome-specific signal ensures the presence of DNA in the specimen even in the absence of the Y-specific signal.
Iida
T
T
Department of Human Genetics, School of International Health, University of Tokyo, Japan.
Nakahori
Y
Y
Tanaka
K
K
Tsutsumi
O
O
Taketani
Y
Y
Nakagome
Y
Y
D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites; D012732:Sex Determination Analysis / Q000379:methods*; D014998:Y Chromosome
D017931:DNA Primers