An amino-terminal fragment of SV40 T antigen induces cellular DNA synthesis in quiescent rat cells.
SV40 large T antigen can stimulate cellular DNA synthesis in quiescent cells. Here we report that an SV40 mutant, which expresses only the amino-terminal 147 amino acids of this protein and which does not make small t antigen, stimulates cellular DNA synthesis in quiescent rat cells at levels similar to those achieved with serum stimulation. Thus, the amino-terminal 147 amino acids of large T antigen define a domain of this protein that is sufficient for efficient stimulation of cellular DNA synthesis in the absence of small t antigen.
Sompayrac
L
L
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.
Danna
K J
KJ
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000096:biosynthesis / Q000235:genetics*; D002455:Cell Division; D002472:Cell Transformation, Viral / Q000235:genetics*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D015870:Gene Expression; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D017384:Sequence Deletion; D013539:Simian virus 40 / Q000235:genetics*; D013329:Structure-Activity Relationship
D000957:Antigens, Viral, Tumor; D010446:Peptide Fragments; D011994:Recombinant Proteins; D004247:DNA