[Formation of mouse chimeras from early embryonic pluripotential stem cell].
Three chimeric mice were produced by injecting the embryonic stem cell-CCE cells into the cavity of 3.5 day host blastocysts from Kunming and C57BL/6J mice. By analyses of glucose phosphate isomerase (GPI-1), the results show a contribution from CCE cells in heart tissue of female Kunming chimera. Breeding experiments demonstrated that all chimeric mice were fertile but none of 3 chimeras produced germ line transmission.
Song
Z
Z
Department of Biology, Peking University.
Li
Q
Q
Shang
K
K
Wu
H
H
D000818:Animals; D002678:Chimera; D004624:Embryo Transfer; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D033581:Stem Cell Transplantation