A method to detect enteroviruses in sewage sludge-amended soil using the PCR.
PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.
Straub
T M
TM
Department of Soil and Water Science, University of Arizona, Tucson 85721.
Pepper
I L
IL
Abbaszadegan
M
M
Gerba
C P
CP
D004770:Enterovirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012722:Sewage; D012988:Soil Microbiology
D012722:Sewage