title: PAD1 encodes phenylacrylic acid decarboxylase which confers resistance to cinnamic acid in Saccharomyces cerevisiae.
abstract: The yeast enzyme phenylacrylic acid decarboxylase (PAD) confers resistance to phenylacrylic acids. Cinnamic acid (CA)-sensitive mutants lacking PAD activity were isolated and the PAD1 gene was cloned by phenotypic complementation. The nucleotide sequence of the smallest complementing fragment was determined. The predicted 242-amino-acid PAD polypeptide is 48.6% identical to the product of dedF of Escherichia coli. PAD activity and CA resistance, but not steady-state PAD1 mRNA levels, are influenced by mitochondrial genotype. PAD1 is a single-copy gene in the yeast genome and not essential for viability. The PAD1 locus was physically mapped to a position approx. 140 kb from the left end of chromosome IV.
authors.affiliation: Plant Biology Laboratory, Salk Institute, San Diego, CA 92186-5800.
authors.forename: M
authors.initials: M
authors.lastname: Clausen
authors.forename: C J
authors.initials: CJ
authors.lastname: Lamb
authors.forename: R
authors.initials: R
authors.lastname: Megnet
authors.forename: P W
authors.initials: PW
authors.lastname: Doerner
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D002934:Cinnamates / Q000494:pharmacology*; D004271:DNA, Fungal; D004352:Drug Resistance, Microbial / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
chemical_list: D002934:Cinnamates; D004271:DNA, Fungal; D005656:Fungal Proteins; C029010:cinnamic acid; D002262:Carboxy-Lyases; C087194:phenylacrylic acid decarboxylase