title: Application of polymorphic DNA sequences to differentiate the origin of decomposed bovine meat.
abstract: Two bovine meat samples were analysed although the samples were already in a state of advanced decomposition. The isolated DNA was extremely degraded and not suitable for conventional DNA fingerprinting (profiling), thus the polymerase chain reaction (PCR) was applied to screen for a Y-chromosome specific fragment and the loci of the two milk proteins kappa-casein and beta-lactoglobulin. Both samples contained Y-chromosome specific DNA and the kappa-casein genotype AA but they differed at the beta-lactoglobulin locus (BB versus AB). Thus a different origin (carcass) could be verified for the two samples and a subject was exonerated from a case of meat larceny.
authors.affiliation: Department of Animal Breeding, Hohenheim University, Stuttgart, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Wagner
authors.forename: T A
authors.initials: TA
authors.lastname: Schild
authors.forename: H
authors.initials: H
authors.lastname: Geldermann
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005784:Gene Amplification; D005838:Genotype; D008894:Milk Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011180:Postmortem Changes; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014998:Y Chromosome / Q000737:chemistry
chemical_list: D008894:Milk Proteins