[Studies on mechanisms of carcinogenesis in vitro. V. The expression of oncogenes in different clones from BHLB4 and their changes after chemically induced differentiation].
Using dot blot methods to identify the relationship between the oncogene expression and monosomy of chromosome No 15 in BHLB4 and their changes after induced differentiation, the results indicated that in Clone-1 (high frequency of chromosome No 15 monosomy) C-myc, H-ras, K-ras, N-ras and C-fos expression was higher than that in BHLB4. In Clone-4 (low frequency of chromosome No 15 monosomy), N-ras expression was lower than that in BHLB4. Decreased expression of C-myc, H-ras, and N-ras oncogenes could also be observed during cell differentiation induced by buta acid. It is possible that an antioncogene located on chromosome No 15 regulates oncogene expression, and differentiation agents would have obvious effects on it.
Liu
B
B
Institute of Radiation Medicine, CAMS and PUMC, Tianjin.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002461:Cell Line, Transformed; D002999:Clone Cells; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016762:Genes, fos; D016259:Genes, myc; D011905:Genes, ras; D008168:Lung / Q000166:cytology; D008647:Mesocricetus; D011519:Proto-Oncogenes
D002087:Butyrates