Alpha-amylase production in lactose medium by Bacillus circulans ACB.
Alpha-amylase production by Bacillus circulans ACB was studied in various cultural conditions. During nutrient optimisation, it was found that 2% lactose can be utilized by the strain as source of carbon providing better growth and enzyme yields than starch. Ammonium sulfate of the basal medium can be replaced by ammonium nitrate for better growth and alpha-amylase activity. The strain demonstrated significant enhancement in alpha-amylase production when grown at pH 6.6.
Bandyopadhyay
A
A
Department of Botany, School of Life Sciences, Visva-Bharati University, Santiniketan, India.
Pal
S C
SC
Sen
S K
SK
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D050260:Carbohydrate Metabolism; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007785:Lactose / Q000378:metabolism*; D017672:Nitrogen Compounds / Q000378:metabolism; D000516:alpha-Amylases / Q000096:biosynthesis*
D003470:Culture Media; D017672:Nitrogen Compounds; D000516:alpha-Amylases; D007785:Lactose