Flow-cytometric analysis of mepacrine-labelled platelets in patients with end-stage renal failure.
Platelet dysfunction and increased bleeding tendency has been the most consistently described haemostatic abnormality in patients with renal failure. Besides abnormalities in platelet membrane glycoproteins, a reduced amount of platelet-dense granule content has been demonstrated in patients with end-stage renal failure (ESRD) indicating an acquired storage pool deficiency (SPD) present in uraemia. To study dense granules, platelets were labelled with mepacrine, a fluorescent probe which is specifically incorporated into dense bodies. MepaPlatelets of 13 patients with ESRD and of 11 healthy controls were studied. The results showed that mepacrine-labelled platelets of patients with ESRD reveal a significantly (p < 0.05) reduced fluorescence compared to the control group. This implies a reduced number or content of dense granules present in ESRD platelets. Thus, the current data indicate that ESRD is associated with an acquired platelet SPD which may be a useful and rapid method for screening patients with suspected acquired or inherited SPD.
Gawaz
M P
MP
Nephrologische Abteilung, Ludwig-Maximilians Universität, Munich, FRG.
Bogner
C
C
Gurland
H J
HJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D005260:Female; D005434:Flow Cytometry; D006474:Hemorrhagic Disorders / Q000209:etiology / Q000503:physiopathology; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000150:complications; D008297:Male; D008875:Middle Aged; D010981:Platelet Storage Pool Deficiency / Q000097:blood / Q000209:etiology*; D011796:Quinacrine; D014511:Uremia / Q000150:complications
D011796:Quinacrine