title: An improved HPLC method to purify erythrocyte cholesterol for estimation of in vivo cholesterol synthesis using the deuterium method.
abstract: We report on an improved HPLC method to purify erythrocyte cholesterol for use in measuring in vivo cholesterol synthesis from 2H2O. The new procedure uses a smaller compression cartridge column at a flow rate of 3 mL/min. The new method reduces by almost tenfold the amount of solvent required to maintain the analytical purity of the isolated cholesterol.
authors.affiliation: Department of Pediatrics, Baylor College of Medicine, Houston, TX.
authors.forename: W W
authors.initials: WW
authors.lastname: Wong
authors.forename: D L
authors.initials: DL
authors.lastname: Hachey
authors.forename: L L
authors.initials: LL
authors.lastname: Clarke
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Llaurador
authors.forename: W G
authors.initials: WG
authors.lastname: Pond
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D013552:Swine
chemical_list: D002784:Cholesterol; D003903:Deuterium