Expression and regulation of a glutamate receptor subunit by bFGF in oligodendrocyte progenitors.
Oligodendrocyte progenitor cells (O-2A) express both kainate-preferring and AMPA-preferring glutamate receptors (Gallo et al., Soc. Neurosci. Abstr., 18:653, 1992b; Gallo et al., 1994). The expression and regulation of the GluR-4c, AMPA-preferring subunit by growth factors was studied by Northern blot analysis of total RNA from purified rat cortical O-2A progenitors. Differently from cortical neurons, O-2A progenitors only expressed two high molecular weight GluR-4c transcripts (6.2 and 4.2 kb), as observed also in cultured astrocytes. Basic fibroblast growth factor (bFGF) increased GluR-4c transcript levels in O-2A progenitors and its effects were not mimicked by platelet derived growth factor (PDGF) or fetal calf serum. Therefore, bFGF may regulate O-2A progenitor responsiveness to glutamate during development through the expression of glutamate receptor subunits.
Gallo
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Unit on Neurobiology, NICHD, National Institutes of Health, Bethesda, Maryland 20892.
Wright
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McKinnon
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D000818:Animals; D015152:Blotting, Northern; D002540:Cerebral Cortex / Q000166:cytology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D008970:Molecular Weight; D009836:Oligodendroglia / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D017470:Receptors, Glutamate; D016222:Fibroblast Growth Factor 2