title: Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes.
abstract: Flow cytometry and two-colour immunofluorescence were used to detect cytoplasmic bovine viral diarrhoea virus (BVDV) antigen in leukocytes from viraemic cattle. Monoclonal antibody to the p80 protein of BVDV, a non-structural viral antigen, was used to identify the subpopulations of leukocytes in which viral protein synthesis had occurred. Viral antigen was detected in 23% of peripheral blood mononuclear cells. Monocytes were found to have the highest frequency of infection (35%). A higher proportion of CD2+ T cells (23%) were infected, compared with B cells (11%) or WC1+ gamma delta T cells (11%). No significant differences in percentages of different leukocyte subpopulations in blood were detected in persistently viraemic animals compared with controls.
authors.affiliation: AFRC Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, U.K.
authors.forename: P
authors.initials: P
authors.lastname: Sopp
authors.forename: L B
authors.initials: LB
authors.lastname: Hooper
authors.forename: M C
authors.initials: MC
authors.lastname: Clarke
authors.forename: C J
authors.initials: CJ
authors.lastname: Howard
authors.forename: J
authors.initials: J
authors.lastname: Brownlie
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood*; D002417:Cattle; D005434:Flow Cytometry; D007962:Leukocytes / Q000382:microbiology*; D009000:Monocytes / Q000382:microbiology; D017361:Viral Nonstructural Proteins / Q000097:blood*; D014766:Viremia
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017361:Viral Nonstructural Proteins