title: Evaluation of testicular germ cell tumor proliferation using DNA flow cytometry and nucleolar organizer silver staining.
abstract: We studied 41 testicular germ cell tumors (TGCT) using DNA flow cytometry and argyrophilic (silver staining) nucleolar organizer region (Ag-NOR) quantitation to evaluate the proliferative characteristics of TGCT and the significance of those analytical techniques. In nonseminomatous germ cell tumor (NSGCT), the percentage of S + G2M phase cells (%S + G2M) in DNA aneuploid tumor was higher than in DNA of diploid tumors. Likewise, in NSGCT the aneuploid Ag-NOR sites per nucleus (Ag-NOR count) were significantly higher in DNA aneuploid than in DNA diploid tumors. There was also a significant linear correlation between % S + G2M phase cells and the Ag-NOR count in TGCT. However, the DNA ploidy pattern and the % S + G2M phase cells, and Ag-NOR counts, did not correlate significantly with the patient survival rate or clinical stage. In conclusion, our study suggests that Ag-NOR enumeration is an effective technique for assessing cellular proliferative activity that is somewhat less expensive than DNA flow cytometry.
authors.affiliation: Department of Urology, Nagoya University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
authors.forename: K
authors.initials: K
authors.lastname: Okamura
authors.forename: T
authors.initials: T
authors.lastname: Shimoji
authors.forename: K
authors.initials: K
authors.lastname: Miyake
authors.forename: Y
authors.initials: Y
authors.lastname: Yamakawa
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
mesh_terms: D002455:Cell Division; D002822:Choriocarcinoma / Q000737:chemistry / Q000401:mortality / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D018237:Germinoma / Q000737:chemistry / Q000401:mortality / Q000473:pathology*; D006801:Humans; D015993:Life Tables; D016014:Linear Models; D008297:Male; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D011379:Prognosis; D018239:Seminoma / Q000737:chemistry / Q000401:mortality / Q000473:pathology; D016622:Silver Staining; D016019:Survival Analysis; D013724:Teratoma / Q000737:chemistry / Q000401:mortality / Q000473:pathology; D013736:Testicular Neoplasms / Q000737:chemistry / Q000401:mortality / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm