Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells.
The effects of medium pH were tested on calvariae, tibiae, and osteoblast-like cells from chick embryos. Bones and isolated cells were incubated for 5 h or 2 days in Hepes-buffered medium at pH values ranging from 6.8 to 8.2. Osteoblast function was evaluated by lactate production, oxygen consumption, alkaline phosphatase activity (AlPase), Ca and inorganic phosphate (Pi) flux, proline hydroxylation, DNA content, and thymidine incorporation. As medium pH was increased, glycolysis, collagen synthesis, and AlPase increased, while Ca efflux decreased. No effect of pH was seen on mitochondrial activity, Pi efflux, or cell number or proliferation. The importance of glycolysis as an endogenous pH regulator was demonstrated by inhibition with iodoacetic acid or glucose restriction and by adding lactate to the medium. The results suggest that the pH of bone interstitial fluid may be regulated by glycolysis and that changes in pH of this compartment may have marked effects on osteoblast function.
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Baxter Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232-2861.
Lenz
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Kaysinger
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D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001857:Bone Matrix / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D004247:DNA / Q000378:metabolism; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D006909:Hydroxyproline / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008903:Minerals / Q000378:metabolism*; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology
D007773:Lactates; D008903:Minerals; D019344:Lactic Acid; D004247:DNA; D000469:Alkaline Phosphatase; D006909:Hydroxyproline