title: Competitive in situ hybridization in a mediastinal germ cell tumor.
abstract: We performed competitive in situ hybridization in a mediastinal germ cell tumor to identify chromosome 12 aberration(s). DNA from a mouse-human somatic hybrid cell line, containing an i(12p) as unique human material, was labeled and used as a probe. Our results confirm that the chromosome marker, observed in mediastinal germ cell tumor and cytogenetically identified as i(12p), is a true isochromosome of the short arm of chromosome 12.
authors.affiliation: Center for Human Genetics, University Hospital Gasthuisberg, Belgium.
authors.forename: M S
authors.initials: MS
authors.lastname: Aly
authors.forename: P
authors.initials: P
authors.lastname: Dal Cin
authors.forename: P
authors.initials: P
authors.lastname: Jiskoot
authors.forename: G
authors.initials: G
authors.lastname: Deneffe
authors.forename: P
authors.initials: P
authors.lastname: Marynen
authors.forename: H
authors.initials: H
authors.lastname: Van den Berghe
mesh_terms: D000293:Adolescent; D000818:Animals; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D025063:Chromosome Disorders; D002881:Chromosomes, Human, Pair 12; D018237:Germinoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008479:Mediastinal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D051379:Mice