title: Crystallization and preliminary X-ray analysis of a lipase from Bacillus subtilis.
abstract: Single crystals of the lipase from Bacillus subtilis have been obtained using a mixture of polyethylene glycol 4000 and sodium sulphate solution as the precipitant. The crystals grow at room temperature in two to three weeks in the presence of n-octyl-beta-D-glucoside. They belong to the monoclinic space group C2 with a = 121.20 A, b = 93.19 A, c = 80.96 A, and beta = 110.67 degrees, with four protein molecules per asymmetric unit. The crystals diffract to at least 2.5 A resolution and are suitable for an X-ray structure analysis.
authors.affiliation: Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Ransac
authors.forename: M
authors.initials: M
authors.lastname: Blaauw
authors.forename: E
authors.initials: E
authors.lastname: Lesuisse
authors.forename: K
authors.initials: K
authors.lastname: Schanck
authors.forename: C
authors.initials: C
authors.lastname: Colson
authors.forename: B W
authors.initials: BW
authors.lastname: Dijkstra
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008049:Lipase / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D008049:Lipase