[Determination on of C/EBP in human liver cancer, normal adult liver and various diseased liver tissues].
C/EBP, a heat-stable DNA-binding protein, play a very important role in establishing and maintaining the differentiation state of liver cells as a transcription factor enriched in the liver. In order to identify its distribution, localization and function, immunocytochemical examination (ABC method) was done by using anti-C/EBP polypeptide antibodies 1103#, 425# in 20 normal human adult liver tissues, 5 neonatal liver tissues, 6 hepatitis tissues, 25 liver cirrhosis tissues, 80 hepatocellular carcinoma tissues (40 cases with associated surrounding non-tumor hepatic tissues) and 26 cholangiocarcinoma tissues (15 cases with associated surrounding non-tumor tissues). The results showed that C/EBP expression was restricted to terminally differentiated liver cells, and was very low or undetectable in poorly differentiated liver cells, such as liver tumor cells. Its expression also correlated with the grading of hepatocellular carcinoma. Besides, positive C/EBP stain could be found in all regenerating bile ductules. The C/EBP has a diffuse distribution which could be detected both in nuclei and in cytoplasm, but more abundant in cytoplasm. The results are in accordance with the concept that C/EBP plays an important role in establishing and maintaining the differentiation state of liver cells.
Xu
L X
LX
Department of Pathology, Fourth Military Medical University, Xi'an.
D000328:Adult; D001650:Bile Duct Neoplasms / Q000737:chemistry; D001653:Bile Ducts, Intrahepatic; D022762:CCAAT-Enhancer-Binding Proteins; D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D018281:Cholangiocarcinoma / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000032:analysis*; D006525:Hepatitis, Viral, Human / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D008099:Liver / Q000737:chemistry*; D008103:Liver Cirrhosis / Q000378:metabolism; D008113:Liver Neoplasms / Q000737:chemistry*; D009687:Nuclear Proteins / Q000032:analysis*
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins