A myeloid-lineage-specific enhancer upstream of the mouse myeloperoxidase (MPO) gene.
The myeloperoxidase (MPO) gene is selectively expressed during haemopoiesis in the granulocytic lineage. Compared with the erythroid (beta-globin) and B-cell (immunoglobulin) lineages, little is known of the regulatory sequences and transcription factors involved in the regulation of genes specific for granulopoiesis. We have approached this issue by identifying a strong enhancer for the murine MPO gene. A candidate enhancer region was mapped by the detection of a strong DNase I hypersensitive site, -3.4 to -3.2 kb upstream of the MPO gene. A 301 bp fragment encompassing the DNase I site was shown to have strong enhancer function in a transient assay following transfection of a reporter gene into a MPO-expressing cell (WEHI 3BD+), but was inactive in lymphoid cells. Analysis of sub-fragments revealed that the whole 301 bp fragment is required for maximal enhancer function.
Zhu
J
J
Section of Cell and Molecular Biology, Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
Bennett
C A
CA
MacGregor
A D
AD
Greaves
M F
MF
Goodwin
G H
GH
Ford
A M
AM
D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000473:pathology*; D002874:Chromosome Mapping; D004742:Enhancer Elements, Genetic; D006410:Hematopoiesis / Q000235:genetics*; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000235:genetics*; D014162:Transfection
D009195:Peroxidase