Effects of acrylamide and acrylic acid on creatine kinase activity in the rat brain.
In vitro, both acrylamide and acrylic acid inhibited creatine kinase (CK) activity in rat brain homogenates, and acrylic acid was more potent than acrylamide. In vivo, however, when given i.p. 50 mg/kg per day for 8 days to rats, only acrylamide inhibited CK activity in the brain and caused apparent neurological signs. 14C in the brain 24h after the injection of 14C-labelled chemicals was more than 7 times greater with acrylamide than with acrylic acid. The inhibition of CK activity by acrylamide varied in eight regions of the brain; from 54% in hypothalamus to 27% in cerebellar vermis. The regional difference of CK inhibition, however, did not agree well with either 14C distribution or with the distribution in regions which appear clinically or pathologically vulnerable to acrylamide.
Kohriyama
K
K
Department of Environmental Toxicology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Matsuoka
M
M
Igisu
H
H
D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D000179:Acrylates / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002250:Carbon Radioisotopes; D003402:Creatine Kinase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D051381:Rats
D000178:Acrylamides; D000179:Acrylates; D002250:Carbon Radioisotopes; D020106:Acrylamide; D003402:Creatine Kinase; C036658:acrylic acid