Partial purification and characterization of Borna disease virions released from infected neuroblastoma cells.
Borna disease is a rare but severe neurological disease of horses and sheep. Borna disease virus (BDV) has not been fully characterized because cell-free virus has not been isolated. Homogenates of infected brain are infectious both for animals and for some cell lines in culture. We report here the partial purification and characterization of cell-free BDV from the tissue culture supernatant of infected human neuroblastoma SKNSH cells. A single negative strand 10-kb RNA was detected in purified virions. Immunoprecipitation analysis of the BDV proteins in purified virions shows the presence of the 60-, 38-, 24-, and 14-kDa proteins previously identified as BDV-specific proteins in infected cells.
Pyper
J M
JM
Division of Comparative Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Clements
J E
JE
D001891:Borna disease virus / Q000235:genetics / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D009447:Neuroblastoma / Q000382:microbiology; D014407:Tumor Cells, Cultured; D014771:Virion / Q000302:isolation & purification