[Purification and physicochemical characterization of allergen I from Dermatophagoides farinae].
Dermatophagoides farinae allergen I (Der f I) has been extracted and purified from spent culture medium of Dermatophagoides farinae by affinity chromatography coupled with anti-Der f I McAb for the first time in China. Furthermore, its physicochemical properties were analysed. By SDS-PAGE, Der f I was estimated to have a molecular weight of 24 kDa and appeared as only one protein band. By isoelectric focusing, it was revealed to have pIs of 4.8-7.5 with six protein bands, suggesting that Der f I is heterogeneous with complicated composition.
Zhong
J M
JM
Medical Acarology Laboratory, Shanghai Medical University.
Wen
T H
TH
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D008925:Mites / Q000737:chemistry* / Q000276:immunology; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D000941:Antigens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins