[The establishment of genomic DNA libraries for the human malaria parasite Plasmodium falciparum].
The DNA of Plasmodium falciparum has been purified and fragmented with restriction endonuclease BamHI. The fragments have been incorporated in vitro into derivatives of bacteriophage lambda EMBL4 digested with BamHI and Sal I. The recombinant mixture has been ligated and packaged in vitro. The recombinant phages have been identified in E. coli L95 host cell and the libraries have been established in which most of the parasite DNA is represented. The ligation proportion of vector to insert is 3:1. The recombinant phages of 4 x 10(5) have been obtained. By plaque hybridization, we have been able to recover from these libraries specific clones containing repetitive DNA sequences.
Liu
K Y
KY
Shandong Institute of Parasitic Diseases, Jining.
Zheng
H G
HG
Huang
P S
PS
Wang
B
B
Cheng
Y L
YL
D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D015698:Genomic Library; D010963:Plasmodium falciparum / Q000235:genetics*
D016054:DNA, Protozoan; D004274:DNA, Recombinant