Okadaic acid induces dephosphorylation of histone H1 in metaphase-arrested HeLa cells.
It is shown here that treatment of metaphase-arrested HeLa cells with okadaic acid (0.15-2.5 microM) leads to dephosphorylation of histone H1. This effect is presumably due to the specific ability of okadaic acid to inhibit protein phosphatases 1 and/or 2A, because okadaic acid tetraacetate, which is not a phosphatase inhibitor, has no effect. Dephosphorylation of H1 does not occur if okadaic acid-treated cells are simultaneously treated with 20 nM calyculin A, or if the okadaic acid concentration is 5.0 microM or greater. The mechanism behind this phenomenon is not known. However, the results suggest that the chain of events leading to histone dephosphorylation may be negatively controlled by a protein phosphatase 2A, while the phosphatase which actually dephosphorylates H1 could be a protein phosphatase 1. It remains to be determined whether the phosphatase involved here is the same enzyme as that which dephosphorylates H1 at the end of normal mitosis.
Paulson
J R
JR
Department of Chemistry, University of Wisconsin-Oshkosh 54901.
Ciesielski
W A
WA
Schram
B R
BR
Mesner
P W
PW
D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D004988:Ethers, Cyclic / Q000494:pharmacology*; D006367:HeLa Cells; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D007700:Kinetics; D008677:Metaphase / Q000187:drug effects / Q000502:physiology*; D008938:Mitosis / Q000187:drug effects / Q000502:physiology; D008940:Mitotic Index / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology; D019319:Okadaic Acid; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors*
D004988:Ethers, Cyclic; D006657:Histones; D007527:Isoenzymes; D019319:Okadaic Acid; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D017027:Protein Tyrosine Phosphatases; D015739:Nocodazole