title: A partial connectin cDNA encoding a novel type of RSP motifs isolated from chicken embryonic skeletal muscle.
abstract: A partial cDNA encoding 811 amino acids of connectin (titin), a giant elastic protein of muscle (3,000 kDa), was cloned from a chicken embryonic skeletal muscle cDNA library using antibodies to muscle connectin. The encoded product was the C terminal segment of connectin. The predicted sequences consisted of 5 type II motifs (immunoglobulin C2 type) separated by 5 interdomain insertions. One interdomain insertion had significant homology (RSP) to KSP repeats found in human cardiac C-terminal connectin and another had a high sequence homology to porin (67.7%; 31 amino acids).
authors.affiliation: Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science.
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: H
authors.initials: H
authors.lastname: Kume
authors.forename: Y
authors.initials: Y
authors.lastname: Kawamura
authors.forename: N
authors.initials: N
authors.lastname: Kanzawa
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Kawashima
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002642:Chick Embryo; D003001:Cloning, Molecular; D064211:Connectin; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics; D009132:Muscles / Q000196:embryology; D011494:Protein Kinases; D017386:Sequence Homology, Amino Acid
chemical_list: D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins; C577285:TTN protein, human; D011494:Protein Kinases