title: Cure of xenografted human tumors by bispecific monoclonal antibodies and human T cells.
abstract: Tumor immunotherapy should increase both the number of T cells that kill the tumor and the likelihood that those cells are activated at the tumor site. Bispecific monoclonal antibodies (Bi-mAbs) were designed that bound to a Hodgkin's tumor-associated antigen (CD30) on the tumor and to either CD3 or CD28 on the T cell. Immunodeficient mice were cured of established human tumors when mice were treated with both the CD3-CD30 and the CD28-CD30 Bi-mAbs and then given human peripheral blood lymphocytes that had been incubated with the CD3-CD30 Bi-mAb and cells that expressed CD30. The enrichment of human T cells within the tumor and the fact that established tumors can be cured may indicate in situ activation of both the T cell receptor and the costimulatory pathway.
authors.affiliation: Medizinische Klinik und Poliklinik, Universität des Saarlandes, Homburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Renner
authors.forename: W
authors.initials: W
authors.lastname: Jung
authors.forename: U
authors.initials: U
authors.lastname: Sahin
authors.forename: R
authors.initials: R
authors.lastname: Denfeld
authors.forename: C
authors.initials: C
authors.lastname: Pohl
authors.forename: L
authors.initials: L
authors.lastname: Trümper
authors.forename: F
authors.initials: F
authors.lastname: Hartmann
authors.forename: V
authors.initials: V
authors.lastname: Diehl
authors.forename: R
authors.initials: R
authors.lastname: van Lier
authors.forename: M
authors.initials: M
authors.lastname: Pfreundschuh
mesh_terms: D000818:Animals; D018033:Antibodies, Bispecific / Q000276:immunology / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal; D018106:CD28 Antigens / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology*; D006689:Hodgkin Disease / Q000276:immunology / Q000628:therapy*; D006801:Humans; D017730:Ki-1 Antigen / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014183:Transplantation, Heterologous
chemical_list: D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D017252:CD3 Complex; D017730:Ki-1 Antigen; D011948:Receptors, Antigen, T-Cell