Serum immunoglobulins interact with oligonucleotides.
The interaction of reactive derivatives of oligonucleotides bearing a 4-[(N-2-chloroethyl-N-methyl)amino]benzylamin residue at the 5'-terminal phosphate with serum blood proteins has been investigated. It was found that the compounds react with serum albumin and immunoglobulins M and G, the reactivity increasing in the order: albumin < IgG < IgM. The reactions with immunoglobulins were inhibited in the presence of different oligonucleotides, DNA and heparin, suggesting the oligonucleotide binding to some cationic region of the proteins. Myoglobin inhibited the interaction of oligonucleotide derivatives with myoglobin-specific monoclonal antibodies which indicates that the derivatives interact with the proteins within or near the antigen binding site.
Rykova EYu
Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk.
Pautova
L V
LV
Yakubov
L A
LA
Karamyshev
V N
VN
Vlassov
V V
VV
D000911:Antibodies, Monoclonal / Q000378:metabolism; D001483:Base Sequence; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism*; D004247:DNA / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000276:immunology / Q000494:pharmacology; D009841:Oligonucleotides / Q000378:metabolism* / Q000494:pharmacology; D012709:Serum Albumin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009211:Myoglobin; D009841:Oligonucleotides; D012709:Serum Albumin; D006493:Heparin; D004247:DNA