Calcium efflux from cultured bovine adrenal chromaffin cells induced by bradykinin.
The effect of bradykinin on Ca2+ efflux from cultured bovine adrenal chromaffin cells was examined. Bradykinin enhanced the efflux of 45Ca2+ from the cells in a concentration dependent manner (10(-9)-10(-6) M). This effect was inhibited by a specific bradykinin B2-receptor antagonist, but not by a B1-receptor antagonist. Nifedipine, Co2+ and Cd2+ did not inhibit the bradykinin-stimulated 45Ca2+ efflux from the cells. 12-O-Tetradecanoyl phorbol 13-acetate, an activator of protein kinase C, also had no effect on the efflux of 45Ca2+ from the cells. The increase in bradykinin-stimulated 45Ca2+ efflux was reduced by removal of extracellular Na+. These results suggest that bradykinin stimulates Na+/Ca2+ exchange in cultured bovine adrenal chromaffin cells.
Houchi
H
H
Department of Pharmacology, Tokushima University School of Medicine, Japan.
Masuda
Y
Y
Ishimura
Y
Y
Ohuchi
T
T
Murakumo
Y
Y
Oka
M
M
D000109:Acetylcholine / Q000494:pharmacology; D000313:Adrenal Medulla / Q000378:metabolism*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D065168:Bradykinin Receptor Antagonists; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D006632:Histamine / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism
D065168:Bradykinin Receptor Antagonists; D000584:Amiloride; D006632:Histamine; D012964:Sodium; D000109:Acetylcholine; D001920:Bradykinin; D002118:Calcium