cDNA cloning and regional distribution of a novel member of the opioid receptor family.
We have cloned a cDNA for a novel member of the opioid receptor family, designated as ROR-C, from the rat cerebrum cDNA library using the probe derived from the delta-opioid receptor subtype cDNA. The deduced amino acid sequence of ROR-C shows high homology with those of ROR-A (rat delta-opioid receptor subtype), ROR-B (rat mu-subtype) and ROR-D (rat kappa-subtype). RNA blot hybridization and in situ hybridization analysis revealed that ROR-C mRNA is expressed in discrete regions of the rat central nervous system.
Fukuda
K
K
Department of Anesthesia, Kyoto University Hospital, Japan.
Kato
S
S
Mori
K
K
Nishi
M
M
Takeshima
H
H
Iwabe
N
N
Miyata
T
T
Houtani
T
T
Sugimoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001933:Brain Stem / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007031:Hypothalamus / Q000378:metabolism; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011957:Receptors, Opioid / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D012333:RNA, Messenger; D011957:Receptors, Opioid; C539902:Rorc protein, rat