title: Interesting features of an activated carbon supported urease system.
abstract: Spherical activated carbon was employed as a new support material for the immobilization of urease. Activity and kinetic behavior of the enzyme were observed. Urease activity was decreased by 10% for the uncoated samples during the washing process whereas a 30% reduction in activity was observed for the coated samples due to the electrical discharge. Activity versus time plots generally appeared to be s-shaped curves at different initial urea levels. At high substrate concentration the kinetics no longer obeyed the Michaelis-Menten relationship due to the inhibition mechanism and diffusion for the support system.
authors.affiliation: Middle East Technical University, Department of Chemistry, Ankara, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Kibarer
mesh_terms: D002606:Charcoal; D004800:Enzymes, Immobilized; D011199:Potentiometry; D014510:Urease / Q000493:pharmacokinetics
chemical_list: D004800:Enzymes, Immobilized; D002606:Charcoal; D014510:Urease