title: Role of myosin light-chain phosphorylation in guinea pig gallbladder smooth muscle contraction.
abstract: In acetylcholine (ACh)-stimulated gallbladder smooth muscle, we have previously shown that phosphorylation of the 20,000-Da myosin light chains is necessary for the initiation of contraction, that myosin is stably phosphorylated at steady state, and that dephosphorylation of cross bridges is not necessary for the slowing of cross-bridge cycling rates during the period of steady-state isometric stress. The present studies were undertaken to determine whether 1) K+ (60 or 80 mM) or cholecystokinin (CCK, 10(-8) M) stimulation is accompanied by changes in myosin light-chain phosphorylation in gallbladder smooth muscle and 2) dephosphorylated noncycling cross bridges exist in K(+)- or CCK-stimulated gallbladder smooth muscle. Isometric stress, isotonic shortening velocity, and myosin light-chain phosphorylation were determined during contraction with K+ or CCK. Steady-state isometric stress was reached within 2.5 min of stimulation with K+ or CCK and was maintained for the duration of the stimulation. Stimulation with K+ or CCK was associated with rapid increases in myosin light-chain phosphorylation and maintenance of myosin light-chain phosphorylation during the stimulation. In contrast, isotonic shortening velocity was maximal at 1 min of stimulation with either K+ or CCK and then declined significantly to values that were only 26-32% of the peak velocity. These data, along with data from previous experiments with ACh, suggest that myosin light-chain phosphorylation is essential in the initiation of contraction in gallbladder smooth muscle, regardless of the source of Ca2+ or of the contractile agonist.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Clinical Studies, University of Pennsylvania School of Veterinary Medicine, Philadelphia.
authors.forename: R J
authors.initials: RJ
authors.lastname: Washabau
authors.forename: M B
authors.initials: MB
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Dorst
authors.forename: J P
authors.initials: JP
authors.lastname: Ryan
mesh_terms: D000818:Animals; D005260:Female; D005704:Gallbladder / Q000502:physiology*; D006168:Guinea Pigs; D006706:Homeostasis; D007537:Isometric Contraction; D009119:Muscle Contraction / Q000502:physiology*; D009130:Muscle, Smooth / Q000502:physiology*; D009218:Myosins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology; D013997:Time Factors
chemical_list: D009218:Myosins; D011188:Potassium