Role of leukocyte function-associated antigen-1 and very late antigen-4 in the adhesion and transmigration of c-myc-transfected B-lymphoblastoid cell lines across vascular endothelium.
The present study was designed to characterize the adhesive interaction of c-myc-transfected B-lymphoblastoid cell lines with resting and interleukin-1-activated endothelial cells. The transfected cell lines expressed lower levels of leukocyte function-associated antigen-1 compared with control non-transfected lines, while no reduction of expression of other surface structures, including the beta 1 integrin very late antigen-4, was observed. The transfected cell lines adhered to resting or activated endothelial cells less than control cells. Anti-CD18 monoclonal antibody inhibited binding of control cell lines but had a modest or no effect on adhesion of transfected cell lines. Anti-very late antigen-4 monoclonal antibody effectively inhibited binding of both transfected and control cell lines; this was more pronounced in the presence of anti-CD18, suggesting a cooperative interaction between these adhesion pathways. Transfected cell lines also had an impaired ability to penetrate endothelial cell monolayers in a transmigration assay. Our results indicate that activation of the c-myc oncogene in B-cells causes alterations in the adhesive interaction with endothelial cells. This may be relevant in the localization and malignant behavior of B-cell lymphomas carrying an activated c-myc oncogene.
Paganin
C
C
Immunology Laboratory, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Bianchi
G
G
Lombardi
L
L
Dalla Favera
R
R
Mantovani
A
A
Allavena
P
P
D001402:B-Lymphocytes / Q000502:physiology*; D002448:Cell Adhesion; D002461:Cell Line, Transformed; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000166:cytology*; D016259:Genes, myc; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D016271:Proto-Oncogene Proteins c-myc / Q000502:physiology; D016029:Receptors, Very Late Antigen / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D014162:Transfection
D016169:Lymphocyte Function-Associated Antigen-1; D016271:Proto-Oncogene Proteins c-myc; D016029:Receptors, Very Late Antigen; D011993:Recombinant Fusion Proteins