The role of receptors of the plasma membrane and nuclear envelope in the failure of insulin imprinting in starving Tetrahymena.
Starvation for 2 h does not disturb the insulin binding of Tetrahymena. At the same time imprinting does not develop at the first encounter with insulin and a down regulation is observed after 168 h. Starvation for 2 h reduces the insulin binding of the nucleus to the half value after starvation for 24 h and this change in binding becomes settled by 48 h. Imprinting is not elicited either in the nuclear membrane. The down-regulation can be observed also after 48 h but it is not present in the 168-h measurements. The experiments emphasize the increased sensitivity of binding sites of the nuclear envelope and the role of it in the development of imprinting.
Csaba
G
G
Department of Biology, Semmelweis University Medical School, Budapest, Hungary.
Hegyesi
H
H
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D009685:Nuclear Envelope / Q000378:metabolism*; D011972:Receptor, Insulin / Q000502:physiology*; D013217:Starvation; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000502:physiology*
D007328:Insulin; D011972:Receptor, Insulin