title: Comparison of nonhuman primate antibodies against Haemophilus influenzae type b polysaccharide with human antibodies in oligoclonality and in vivo protective potency.
abstract: Nonhuman primates are often used as a model for studying vaccines for humans. However, it is not always clear how closely the antibody responses in these species mimic human responses. Recent studies have characterized the human antibody response to Haemophilus influenzae type b (Hib) in great detail. In this study, we have compared the antibody response to Hib of humans with those of other primates. Studies of isoelectric points and V kappa subgroup usage show that, like humans, nonhuman primates produce oligoclonal antibodies. Also, monkey antibodies to the Hib polysaccharide are as protective as human antibodies in an in vivo model of Hib infection. Thus, we conclude that nonhuman primates produce antibodies to Hib polysaccharide that are structurally and functionally similar to human antibodies and are a good model for testing human vaccines.
authors.affiliation: Unit for Bacterial Vaccine Development and Pathogenesis Research, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: M K
authors.initials: MK
authors.lastname: Park
authors.forename: C C
authors.initials: CC
authors.lastname: Peeters
authors.forename: J T
authors.initials: JT
authors.lastname: Poolman
authors.forename: M H
authors.initials: MH
authors.lastname: Shearer
authors.forename: R C
authors.initials: RC
authors.lastname: Kennedy
authors.forename: M H
authors.initials: MH
authors.lastname: Nahm
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology*; D016667:Bacterial Capsules; D018073:Haemophilus Vaccines / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000032:analysis; D008252:Macaca fascicularis; D010215:Papio; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000907:Antibodies, Bacterial; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D007132:Immunoglobulin Isotypes; D011135:Polysaccharides, Bacterial