The effect of sodium concentration on myocardial viability in donor heart preservation using a nondepolarizing solution.
This study examined the effect of different sodium concentrations in a nondepolarizing solution on myocardial viability and functional recovery of the canine donor heart. Isolated canine hearts were preserved for 6 h at 5 degrees C, followed by normothermic reperfusion for 2 h. Dogs were divided into two groups of nine dogs each: group 1 received a nondepolarizing solution with 70 mM Na+ and group 2 with 30 mM Na+. The myocardial Ca2+ concentration at the end of preservation was significantly higher in group 1 than in group 2 and increased after reperfusion in both groups without any intergroup difference. Myocardial concentrations of ATP, ADP, and total adenine nucleotide at the end of reperfusion were significantly higher in group 1 than in group 2. Myocardial cyclic adenosine monophosphate concentration was significantly higher in group 1 than in group 2 at the end of both preservation and reperfusion. The myocardial cyclic guanosine monophosphate concentration in group 1 increased and was higher than in group 2 at the end of preservation, but had returned to normal levels by the end of reperfusion. However, it remained unchanged through preservation and reperfusion in group 2. The left ventricular systolic and diastolic function, assessed by pressure-volume relationship, was better in group 1 than in group 2. Mitochondrial ultrastructural changes were similar. These results suggest that a nondepolarizing solution containing 70 mM Na+ provides better myocardial protection than a solution containing 30 mM Na+.
Sunamori
M
M
Department of Thoracic-Cardiovascular Surgery, Tokyo Medical and Dental University, Japan.
Shirai
T
T
Amano
J
J
Miyamoto
H
H
Suzuki
A
A
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002314:Cardioplegic Solutions / Q000494:pharmacology*; D002470:Cell Survival; D015925:Cryopreservation; D000242:Cyclic AMP / Q000378:metabolism; D004285:Dogs; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D008929:Mitochondria, Heart / Q000648:ultrastructure; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009926:Organ Preservation / Q000379:methods*; D012964:Sodium / Q000494:pharmacology*; D014019:Tissue Donors
D000227:Adenine Nucleotides; D002314:Cardioplegic Solutions; D012964:Sodium; D000242:Cyclic AMP; D002118:Calcium