title: Photinus pyralis luciferase: vectors that contain a modified luc coding sequence allowing convenient transfer into other systems.
abstract: The coding sequence of the Photinus pyralis luciferase (Luc), as contained in the previously described expression vector, pUHC13-1, was modified such that several commonly used restriction sites in the luc gene were removed without changing the primary sequence of the protein. In addition, a Kozak sequence upstream from the ATG start codon was introduced. The resulting plasmid, pUHC131-1, produced increased amounts of Luc activity in HeLa and NIH3T3 cells. The modified luc gene was inserted in both orientations into the multiple cloning site of a commonly used cloning vector. This facilitates transfer of luc into other expression units.
authors.affiliation: Zentrum für Molekulare Biologie, Universität Heidelberg (ZMBH), Germany.
authors.forename: A L
authors.initials: AL
authors.lastname: Bonin
authors.forename: M
authors.initials: M
authors.lastname: Gossen
authors.forename: H
authors.initials: H
authors.lastname: Bujard
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D001517:Coleoptera / Q000201:enzymology* / Q000235:genetics; D017344:Genes, Insect; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D008156:Luciferases