title: Maternal contamination of amniotic fluid demonstrated by DNA analysis.
abstract: DNA from 16 sets of samples comprising DNA from uncultured amniotic fluid cells, cultured amniotic fluid cells, fetal tissue, and maternal blood was analysed by the polymerase chain reaction (PCR) with AC-repeat primers. The analysis was performed to investigate the presence of contaminating maternal cells in amniotic fluid which would affect the reliability of DNA studies for prenatal diagnosis. In three sets, maternal contamination of uncultured amniotic fluid cells was detected. In one of the three sets, maternal contamination was present in both uncultured and cultured amniotic fluid cells. The use of amniotic fluid cells as a source of DNA for prenatal diagnosis should be limited to cases where the purity of the DNA can be demonstrated prior to the diagnostic test being performed. This limitation in the use of amniotic fluid DNA also extends to other forms of diagnosis relying on the purity of amniotic fluid samples, particularly the new in situ hybridization methods currently being developed.
authors.affiliation: Cytogenetics Unit, St Mary's Hospital Medical School, London, U.K.
authors.forename: M T
authors.initials: MT
authors.lastname: Rebello
authors.forename: A
authors.initials: A
authors.lastname: Abas
authors.forename: K
authors.initials: K
authors.lastname: Nicolaides
authors.forename: D V
authors.initials: DV
authors.lastname: Coleman
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry* / Q000166:cytology; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000592:standards; D013048:Specimen Handling
chemical_list: D004247:DNA