[Properties of an acellular preparation of Bordetella pertussis and its analysis after centrifugation in saccharose gradient].
Acellular preparations were studied which were obtained by a method of acidic extraction from culture of strain Tohama of Bordetella pertussis. Obtained preparations EM I and EM II were exhibiting property of leukocytosis stimulation and were respectively of following values: 43.5 and 19 u LPF/mg of protein. They also caused hemagglutination (150 and 19 u HA/mg of protein). Both preparations were exhibiting protective effect in intracerebral test performed on mice. After differentiation of EM I preparation by preparative centrifugation in saccharose gradient two fractions were obtained, of which lighter was exhibiting high hemagglutinating activity and leukocytosis stimulation (198 u LPF/mg of protein). Heaver fraction contained mainly endotoxin. Applied method permitted for partial purification of acellular preparation of B. pertussis; mainly decrease in its content of endotoxin responsible for high reactogenicity of pertussis vaccines.
Letowska
I
I
Centralne Laboratorium Surowic i Szczepionek, Warszawie.
Kaczurba
E
E
Schiller
B
B
D000818:Animals; D001886:Bordetella pertussis / Q000378:metabolism*; D003429:Cross Reactions; D006384:Hemagglutination / Q000276:immunology; D007964:Leukocytosis / Q000276:immunology; D051379:Mice; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000096:biosynthesis; D013395:Sucrose / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000032:analysis
D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; D013395:Sucrose; D037342:Pertussis Toxin
Właściwości bezkomórkowego preparatu Bordetella pertussis i jego analiza po wirowaniu w gradiencie sacharozy.