Detection of hepatitis A virus in clinical and environmental samples by immunomagnetic separation and PCR.
Magnetic beads coated with antibodies against surface epitopes of the hepatitis A virus (HAV) captured efficiently viral particles from various types of samples. Contaminating substances, including particulate material, were removed by using a magnet to retain the beads during the washing procedure. A sensitive reverse transcriptase/nested polymerase chain reaction (RT-PCR) was developed to confirm the presence of viral particles on the beads. The above technique was shown to be useful for the detection of a laboratory strain of HAV added to polluted river water, sea water and fecal extracts.
Monceyron
C
C
Department of Virology, National Institute of Public Health, Oslo, Norway.
Grinde
B
B
D001483:Base Sequence; D017848:Blood-Borne Pathogens / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005618:Fresh Water; D006507:Hepatovirus / Q000302:isolation & purification*; D006801:Humans; D018189:Immunomagnetic Separation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012623:Seawater; D012680:Sensitivity and Specificity; D014871:Water Microbiology