title: Detection of Borrelia burgdorferi antigens in cerebrospinal fluid.
abstract: We examined CSF for Borrelia burgdorferi antigens using antigen-capture ELISA and Western (immuno) blot. Antigen-capture ELISA was positive in 38 of 77 (49%) CSF samples obtained from neurologic patients with presumed B burgdorferi infection, compared with one of 34 (3%) CSF samples obtained from other neurologic disease controls who came from a region endemic for Lyme disease. Western immunoblot was positive for B burgdorferi antigens in 12 of 22 (55%) CSF samples from the B burgdorferi infected groups, compared with none of 11 CSF samples from the control group. CSF antigen detection should prove helpful in evaluating patients for suspected neurologic Lyme disease.
authors.affiliation: Department of Neurology, SUNY at Stony Brook 11794.
authors.forename: P K
authors.initials: PK
authors.lastname: Coyle
authors.forename: Z
authors.initials: Z
authors.lastname: Deng
authors.forename: S E
authors.initials: SE
authors.lastname: Schutzer
authors.forename: A L
authors.initials: AL
authors.lastname: Belman
authors.forename: J
authors.initials: J
authors.lastname: Benach
authors.forename: L B
authors.initials: LB
authors.lastname: Krupp
authors.forename: B
authors.initials: B
authors.lastname: Luft
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000134:cerebrospinal fluid*; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial