[Expression of rat CPSI full-length cDNA gene in non-liver culture cells].
The full-length CPSI cDNA gene (5.65kb in length) was recombined with pSV2 expression vector, and the recombinant pSV2-CPScf plasmid was expressed CPSI in 7402 hepatoma, CHO and NIH/3T3 cell lines. The pSV2-CPScf plasmid was able to express CPSI protein stably and effectively in all three cell lines. The transcriptional product was identified by Northern blot and nuclear 'runoff' transcription in vitro to be full length CPSI mRNA. Post-transcription and translation processing were found to be not indispensible for the tissue-specific control of CPSI gene expression. This study also provides evidence of large molecular cDNA gene expression using the pSV2 vector and provides cultured cell lines producing CPSI protein in non-liver cells.
Niu
B
B
Institute of Basic Medical Sciences, CAMS, Beijing.
D016475:3T3 Cells / Q000378:metabolism; D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D004274:DNA, Recombinant; D015870:Gene Expression; D008113:Liver Neoplasms / Q000378:metabolism; D051379:Mice; D010957:Plasmids; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D004274:DNA, Recombinant; D012333:RNA, Messenger; D002222:Carbamoyl-Phosphate Synthase (Ammonia)