Targeted development of microsatellite markers from inter-Alu amplification of YAC clones.
Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers from targeted regions of the genome. This can be achieved by selectively cloning and sequencing (CA)n-positive human inter-Alu sequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers.
de Souza
A P
AP
Universidade Estadual de Campinas, (SP), Brazil.
Allamand
V
V
Richard
I
I
Brenguier
L
L
Chumakov
I
I
Cohen
D
D
Beckmann
J S
JS
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D005784:Gene Amplification; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite; D005819:Genetic Markers