[The inhibitory effect of mouse monoclonal IgM antibody E5 against endotoxin on limulus activity of various lipopolysaccharides].
The anti-endotoxin activity of mouse monoclonal IgM antibody E5 was determined against various lipopolysaccharides (LPSs) derived from Escherichia coli J5, E. coli O111:B4, Klebsiella pneumoniae and Pseudomonas aeruginosa by measuring limulus activity by endospecy method. In general, above 0.2 microgram/ml of E5 reduced almost dose-dependently the limulus activities of all LPSs tested, although the degree of reduction in limulus activity somewhat varied among bacterial species. The limulus activities of LPS from E. coli J5, E. coli O111:B4, K. pneumoniae and P. aeruginosa decreased by 72%, 50%, 44%, and 58%, respectively, by exposure to 5.0 micrograms/ml of E5 for 2 hrs.
Kobayashi
H
H
First Department of Internal Medicine, Kyourin University School of Medicine.
Oishi
S
S
Koumoto
A
A
Yoshida
T
T
Matsunaga
T
T
Ogawa
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D004926:Escherichia coli; D007075:Immunoglobulin M / Q000494:pharmacology*; D007136:Immunoglobulins / Q000494:pharmacology*; D007711:Klebsiella pneumoniae; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D011550:Pseudomonas aeruginosa
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D007136:Immunoglobulins; D008070:Lipopolysaccharides; C042866:antilipopolysaccharide antibodies