Electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) of multilamellar bodies and multilamellar body-like structures in tannic acid-treated alveolar septal cells.
We used electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) to compare multilamellar bodies (MLB) of Type II alveolar epithelial cells with MLB-like structures that are present in various alveolar septal cells after fixation with tannic acid. Despite their structural similarity in conventional transmission electron microscopy, the phosphorus signal recorded by both ESI and EELS was considerably higher in multilamellar bodies than in MLB-like structures. This indicates that they are different in chemical composition.
Ochs
M
M
Department of Anatomy, University of Göttingen, Germany.
Fehrenbach
H
H
Richter
J
J
D000818:Animals; D047348:Hydrolyzable Tannins / Q000633:toxicity*; D008854:Microscopy, Electron / Q000379:methods; D015388:Organelles / Q000187:drug effects / Q000648:ultrastructure*; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000648:ultrastructure*; D013552:Swine
D047348:Hydrolyzable Tannins