Intragenic recombination in the CSR1 locus of Arabidopsis.
Four classes of herbicides are known to inhibit plant acetolactate synthase (ALS). In Arabidopsis, ALS is encoded by a single gene, CSR1. The dominant csr1-1 allele encodes an ALS resistant to chlorsulfuron and triazolopyrimidine sulfonamide while the dominant csr1-2 allele encodes an ALS resistant to imazapyr and pyrimidyl-oxy-benzoate. The molecular distance between the point mutations in csr1-1 and csr1-2 is 1369 bp. Here we used multiherbicide resistance as a stringent selection to measure the intragenic recombination frequency between these two point mutations. We found this frequency to be 0.008 +/- 0.0028. The recombinant multiherbicide-resistant allele, csr1-4, provides an ideal marker for plant genetic transformation.
Mourad
G
G
Department of Biology, University of Saskatchewan, Saskatoon, Canada.
Haughn
G
G
King
J
J
D000095:Acetolactate Synthase / Q000037:antagonists & inhibitors / Q000235:genetics*; D000483:Alleles; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D001565:Benzoates / Q000494:pharmacology; D003062:Codon; D004252:DNA Mutational Analysis; D017931:DNA Primers; D004351:Drug Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D006540:Herbicides / Q000494:pharmacology; D006720:Homozygote; D007093:Imidazoles / Q000494:pharmacology; D008969:Molecular Sequence Data; D009525:Niacin / Q000031:analogs & derivatives / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics; D017354:Point Mutation; D011995:Recombination, Genetic; D013449:Sulfonamides / Q000494:pharmacology; D014170:Transformation, Genetic; D014227:Triazines / Q000494:pharmacology
D001565:Benzoates; D003062:Codon; D017931:DNA Primers; D005819:Genetic Markers; D006540:Herbicides; D007093:Imidazoles; D010940:Plant Proteins; D013449:Sulfonamides; D014227:Triazines; D009525:Niacin; C076656:imazapyr; D000095:Acetolactate Synthase; C037137:chlorsulfuron