[Use of direct and serologic methods to detect Chlamydia trachomatis infections in cervix uteri of women].
Investigation included 254 women (18-75 years of age). In this number, 74 patients constituted a group with pathological changes in cervix uteri, while 180 women were seemingly healthy. The material was evaluated by a method of direct immunofluorescence (IF) with monoclonal antibody serum and a culture on McCoy cells. Antibodies in blood serum was detected by an immunoenzymatic test ELISA. Out of total number of 256 patients tested, elementary bodies of C. trachomatis were found in cylindrical cells of cervix uteri by the IF test in 54 (21.25%) of women. Analogical percentage of positive results was obtained by application of culture on McCoy cells. Positive serological results were found in 70 patients (27.55%). In group of 75 patients infected with C. trachomatis, 33 cases (44.59%) were confirmed by application of IF and cell culture, whereas positive serological results were found in 39 (52.70%) of investigated patients. In the group of seemingly healthy women in 11.60% elementary bodies of C. trachomatis were detected and in 17.22% of cases serological results were positive.
Kazanowska
W
W
Instytut Połoznictwa i Chorób Kobiecych.
Zdrodowska-Stefanow
B
B
Kuczyńska
K
K
D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D011237:Predictive Value of Tests; D012016:Reference Values; D012698:Serologic Tests; D002575:Uterine Cervicitis / Q000382:microbiology*
Wykrywalność badaniami bezpośrednimi i serologicznymi zakazeń Chlamydia trachomatis szyjki macicy kobiet.