Identification of RAPD primers that reveal extensive polymorphisms between laboratory strains of zebrafish.
The zebrafish has recently emerged as a useful system for understanding vertebrate developmental genetics, despite the lack of a linkage map. To identify DNA-based genetic polymorphisms for constructing a genetic map, we have screened a collection of RAPD primers for their utility in identifying genetic polymorphisms between two laboratory strains of zebrafish. Here, we report 116 primers that identify 721 strain-specific genetic markers and show how they can be used in haploid genetics of zebrafish.
Johnson
S L
SL
Institute of Neuroscience, University of Oregon, Eugene 97403.
Midson
C N
CN
Ballinger
E W
EW
Postlethwait
J H
JH
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D005819:Genetic Markers; D006238:Haploidy; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D013045:Species Specificity; D015027:Zebrafish / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers