title: Discrimination of related transcribed and non-transcribed repetitive DNA sequences from the Y chromosomes of Drosophila hydei and Drosophila eohydei.
abstract: The short arm of the Y chromosome of Drosophila hydei carries a single male fertility gene, gene Q, which forms the lampbrush loop pair Nooses. Conflicting observations have been reported concerning the identity of the repetitive DNA sequences that are transcribed in this loop pair. It has been claimed by other investigators that the loop transcripts contain repeats of two distinct, but related families of Y-specific repetitive DNA sequences, ay1 and YsI. We reinvestigated this issue, using as probes single ay1 and YsI repeats which, under stringent conditions, hybridize only to members of their own family. Under non-stringent conditions, both repeats hybridize in situ to Nooses transcripts. However, if hybridization conditions are stringent, only the ay1 probe hybridizes to loop transcripts. Hybridizations to Northern blots of testis RNA confirm these results. Further, YsI repeats are not found the closely related species D. eohydei. We conclude that the YsI repeats are not relevant for the function of fertility gene Q.
authors.affiliation: Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Hochstenbach
authors.forename: M
authors.initials: M
authors.lastname: Knops
authors.forename: W
authors.initials: W
authors.lastname: Hennig
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D004247:DNA; D004330:Drosophila / Q000235:genetics*; D005298:Fertility / Q000235:genetics; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014998:Y Chromosome
chemical_list: D004247:DNA