title: Molecular cloning and expression of rat prostaglandin E receptor EP2 subtype.
abstract: A cDNA clone encoding the rat prostaglandin (PG) E receptor EP2 subtype was cloned from a rat lung cDNA library. It encodes 488 amino acid residues with putative seven-transmembrane domains. Specific binding of [3H]PGE2 was found in COS-7 cells transfected with the cDNA and was displaced with unlabeled prostaglandins in the order of PGE2 = PGE1 >> iloprost > or = PGF2 alpha > or = PGD2. The binding was also inhibited by misoprostol, an EP2 and EP3 agonist, but not by sulprostone, an EP1 and EP3 agonist. Northern blot analysis demonstrated that the EP2 mRNA is widely expressed in various tissues, the significant expression being observed in the thymus, lung, spleen, heart stomach, and pancreas.
authors.affiliation: Department of Medicine, Kyoto University Faculty of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sando
authors.forename: T
authors.initials: T
authors.lastname: Usui
authors.forename: I
authors.initials: I
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Namba
authors.forename: Y
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: A
authors.initials: A
authors.lastname: Ichikawa
authors.forename: S
authors.initials: S
authors.lastname: Narumiya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015232:Dinoprostone / Q000378:metabolism; D015870:Gene Expression; D005796:Genes; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D018078:Receptors, Prostaglandin E / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E; D011994:Recombinant Proteins; D015232:Dinoprostone