A new tool for investigating G protein-coupled receptors.
Vertebrate olfactory receptors are members of the seven-transmembrane-domain G protein-coupled receptor family. They utilize intracellular signal transduction pathways which are activated by stimulation of odorant receptors and use the second messengers cAMP and/or inositol 1,4,5-trisphosphate and diacylglycerol. Studies of how odorants bind to and activate the receptors can be considered part of the more general problem of how chemicals interact with G protein-coupled receptors. This review describes the development of a new technique for assessing functional interactions between chemicals and these receptors in only minutes. Predicted uses of the system include structure-function analyses of both G protein-coupled receptors and their ligands, studies of receptor coupling to G proteins and cloning of cDNAs for these receptors.
Lerner
M R
MR
Howard Hughes Medical Institute Research Laboratories, Yale University School of Medicine, New Haven, CT 06536-0812.
Potenza
M N
MN
Graminski
G F
GF
McClintock
T
T
Jayawickreme
C K
CK
Karne
S
S
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000378:metabolism*; D018035:Receptors, Odorant / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*
D018035:Receptors, Odorant; D011994:Recombinant Proteins; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases