title: Equipment for the large-scale electromanipulation of plant protoplasts.
abstract: Electric fields are now used extensively for the genetic manipulation of plant cells through protoplast fusion and direct gene uptake. The cost of commercially available electrofusion and electroporation equipment remains prohibitive for many laboratories. This paper describes an electronic apparatus, suitable for the large-scale electrofusion and electroporation of plant protoplasts that is compatible in both function and cost with commercially available equipment.
authors.affiliation: Department of Life Science, University of Nottingham, UK.
authors.forename: B
authors.initials: B
authors.lastname: Jones
authors.forename: P T
authors.initials: PT
authors.lastname: Lynch
authors.forename: G J
authors.initials: GJ
authors.lastname: Handley
authors.forename: R S
authors.initials: RS
authors.lastname: Malaure
authors.forename: N W
authors.initials: NW
authors.lastname: Blackhall
authors.forename: N
authors.initials: N
authors.lastname: Hammatt
authors.forename: J B
authors.initials: JB
authors.lastname: Power
authors.forename: E C
authors.initials: EC
authors.lastname: Cocking
authors.forename: M R
authors.initials: MR
authors.lastname: Davey
mesh_terms: D001698:Biomedical Engineering; D001709:Biotechnology; D002459:Cell Fusion; D004566:Electrodes; D004574:Electromagnetic Fields; D018274:Electroporation / Q000295:instrumentation*; D004869:Equipment Safety; D059828:Plant Cells; D010944:Plants / Q000235:genetics; D011523:Protoplasts / Q000166:cytology*