Identification and characterization of parathyroid hormone/parathyroid hormone-related peptide receptor in cultured astrocytes.
Parathyroid hormone (PTH)/parathyroid hormone-related peptide (PTHrP) receptor expressed in rat cultured cortical type I astrocytes was identified and characterized. Northern blot analysis demonstrated that the PTH/PTHrP receptor mRNA is expressed in astrocytes. A human PTH fragment, PTH [1-34], stimulated adenylate cyclase with the EC50 value of 3 nM, but not phospholipase C. PTH [1-34] changed the morphology of protoplasmic type astrocytes into process-bearing ones. Thus, type I astrocytes possess PTH/PTHrP receptor that is coupled to an adenylate cyclase-activating system but not to phospholipase C.
Hashimoto
H
H
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Aino
H
H
Ogawa
N
N
Nagata
S
S
Baba
A
A
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D010281:Parathyroid Hormone / Q000494:pharmacology; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D011506:Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D044168:Receptor, Parathyroid Hormone, Type 1; D018016:Receptors, Parathyroid Hormone / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D019379:Teriparatide; D010738:Type C Phospholipases / Q000378:metabolism
D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D010716:Phosphatidylinositols; D011506:Proteins; D012333:RNA, Messenger; D044168:Receptor, Parathyroid Hormone, Type 1; D018016:Receptors, Parathyroid Hormone; D019379:Teriparatide; C054296:parathyroid hormone-related protein (1-34); D000242:Cyclic AMP; D010738:Type C Phospholipases; D000262:Adenylyl Cyclases