title: Binding of aluminum to human serum transferrin, human serum albumin and rat serum proteins.
abstract: Human serum transferrin (HSTF), human serum albumin (HSA) and rat serum were compared for their interaction with AlCl3, in a Tris-HCl buffer solutions (pH 7.4). The AlCl3 was tested in series of concentrations in the range of 50 microM up to 500 microM. HSTF, HSA and their 1:1 mixture and rat serum were incubated at 37 degrees C with series of AlCl3 concentrations. The protein profile of the incubated solutions were compared to control using SDS-PAGE and FPLC tests. The results indicated that HSTF was more specifically responsive to AlCl3 showing a characteristic increase in its UV absorption, peak and area dimensions. Simultaneously, HSA was less affected, but it showed a significant shift with an increase in molecular weight accompanied with a change in its profile. The respective bands of transferrin and albumin in rat serum behaved similarly. The SDS-PAGE and FPLC data coincided and confirmed the preferential affinity of HSTF to bind with Al3+. These results support the suggestion of using HSTF for monitoring levels of Al3+ in human blood samples of exposed population. The importance of further developing such a biomarker is the increased demand for early detection of the hazardous levels of Al3+ in relation to its long term neurotoxic adverse effects.
authors.affiliation: Chemistry Department, Texas Southern University, Houston 77004.
authors.forename: A K
authors.initials: AK
authors.lastname: el-Sebae
authors.forename: M M
authors.initials: MM
authors.lastname: Abou Zeid
authors.forename: F H
authors.initials: FH
authors.lastname: Abdel-Rahman
authors.forename: M A
authors.initials: MA
authors.lastname: Saleh
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D002712:Chlorides / Q000097:blood*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004781:Environmental Exposure; D006801:Humans; D007525:Isoelectric Focusing; D011485:Protein Binding; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014168:Transferrin / Q000378:metabolism*
chemical_list: D017607:Aluminum Compounds; D015415:Biomarkers; D001798:Blood Proteins; D002712:Chlorides; D012709:Serum Albumin; D014168:Transferrin; D000077410:Aluminum Chloride