Effects of heparin, venous strangulation obstruction of the small intestine, and reperfusion of the small intestine on plasma diamine oxidase activity in horses.
Diamine oxidase (DAO), an enzyme of small intestinal origin, is released from mucosal storage sites by IV administration of heparin, to yield the plasma postheparin DAO (PHD) curve. The PHD curve is diminished when mucosal surface area is lost, and baseline (without heparin) plasma DAO activity increases when mucosal storage sites are damaged. Plasma DAO activity was measured after 2 doses of heparin were administered IV in healthy, conscious horses. In anesthetized horses, the PHD curve was studied: during sham small intestinal surgery, and during venous strangulation obstruction (VSO) of the distal 50% of the small intestine. In a third group of anesthetized horses, baseline plasma DAO activity (without heparin) was measured during VSO of the distal 50% of the small intestine for 90 minutes, followed by reperfusion for 90 minutes. Postheparin plasma DAO curves in conscious horses were similar to those reported in other species. Horses with VSO had a similar PHD curve as did sham-operated controls at all times, except at 15 minutes, when plasma DAO activity was significantly (P < 0.05) greater in the VSO group. Horses with VSO and reperfusion had no change in baseline plasma DAO activity throughout the study. Peritoneal fluid DAO activity remained low throughout the study, but increased slightly in horses with VSO that received heparin, possibly because of DAO from extravasated blood in the peritoneal fluid. Results indicated that the plasma DAO response to IV administered heparin in horses is similar to that in other mammals, but, unlike other species, baseline and postheparin DAO activities did not change as expected after small intestinal vascular obstruction and mucosal injury.(ABSTRACT TRUNCATED AT 250 WORDS)
Laws
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Department of Clinical Studies, University of Pennsylvania, School of Veterinary Medicine, New Bolton Center, Kennett Square 19348.
Freeman
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D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D000818:Animals; D000990:Antithrombin III / Q000032:analysis; D005260:Female; D006493:Heparin / Q000494:pharmacology*; D006736:Horses / Q000097:blood*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology*; D007415:Intestinal Obstruction / Q000097:blood / Q000201:enzymology*; D007421:Intestine, Small / Q000098:blood supply*; D007700:Kinetics; D008297:Male; D009130:Muscle, Smooth / Q000098:blood supply*; D010314:Partial Thromboplastin Time; D010976:Platelet Count; D013997:Time Factors
D000990:Antithrombin III; D006493:Heparin; D006631:Amine Oxidase (Copper-Containing)