title: Core histone hyperacetylation co-maps with generalized DNase I sensitivity in the chicken beta-globin chromosomal domain.
abstract: The distribution of core histone acetylation across the chicken beta-globin locus has been mapped in 15 day chicken embryo erythrocytes by immunoprecipitation of mononucleosomes with an antibody recognizing acetylated histones, followed by hybridization probing at several points in the locus. A continuum of acetylation was observed, covering both genes and intergenic regions. Using the same probes, the generalized sensitivity to DNase I was mapped by monitoring the disappearance of intact genomic restriction fragments from Southern transfers. Close correspondence between the 33 kb of sensitive chromatin and the extent of acetylation indicates that one role of the modification could be the generation and/or maintenance of the open conformation. The precision of acetylation mapping makes it a possible approach to the definition of chromosomal domain boundaries.
authors.affiliation: Biophysics Laboratories, University of Portsmouth, UK.
authors.forename: T R
authors.initials: TR
authors.lastname: Hebbes
authors.forename: A L
authors.initials: AL
authors.lastname: Clayton
authors.forename: A W
authors.initials: AW
authors.lastname: Thorne
authors.forename: C
authors.initials: C
authors.lastname: Crane-Robinson
mesh_terms: D000107:Acetylation; D000818:Animals; D001773:Blood Cells; D002642:Chick Embryo; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006657:Histones / Q000737:chemistry* / Q000276:immunology; D009707:Nucleosomes; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D005914:Globins; D003850:Deoxyribonuclease I