title: Downregulation of adenylylcyclase types V and VI mRNA levels in pacing-induced heart failure in dogs.
abstract: We have shown that the heart expresses two distinct forms of adenylylcyclase mRNA, types V and VI. In this study we have characterized the expression of these two mRNA species in heart failure generated by overdrive pacing at a rate of 240 beats/min. After 4 wk, left ventricular end-diastolic pressure and heart rate increased significantly with the appearance of signs of heart failure, i.e., edema, ascites, and exercise intolerance. Basal as well as forskolin-stimulated adenylylcyclase activities decreased significantly, which was accompanied by a reduction in the steady state mRNA levels of adenylylcyclase types V and VI. These data suggest that in this model of cardiomyopathy, the downregulation of adenylylcyclase catalytic activity results, at least in part, from a reduction in the steady state levels of types V and VI adenylylcyclase mRNA levels.
authors.affiliation: Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York 10032.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: S
authors.initials: S
authors.lastname: Sorota
authors.forename: K
authors.initials: K
authors.lastname: Kiuchi
authors.forename: R P
authors.initials: RP
authors.lastname: Shannon
authors.forename: K
authors.initials: K
authors.lastname: Komamura
authors.forename: S
authors.initials: S
authors.lastname: Katsushika
authors.forename: D E
authors.initials: DE
authors.lastname: Vatner
authors.forename: S F
authors.initials: SF
authors.lastname: Vatner
authors.forename: C J
authors.initials: CJ
authors.lastname: Homcy
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics*; D000818:Animals; D004285:Dogs; D015536:Down-Regulation; D005260:Female; D006333:Heart Failure / Q000201:enzymology*; D006339:Heart Rate; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis*; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D000262:Adenylyl Cyclases