Relationship of a dystrophin-associated glycoprotein to junctional acetylcholine receptor clusters in rat skeletal muscle.
The relationship of a member of the transmembrane dystrophin-associated glycoprotein (DAG) complex to acetylcholine receptors (AChRs) was investigated using immunofluorescence techniques at rat neuromuscular junctions (NMJs) viewed en face. These results were compared with those from a similar previous study of dystrophin and an autosomal homologue (utrophin or dystrophin-related protein, DRP) (Bewick et al. Neuro Report 1992; 3: 857-860). The region of highest 43 K DAG (43DAG) labelling projected beyond the AChRs by approximately 0.3 microns, as does that for dystrophin. By contrast DRP labelling precisely co-localizes with the AChRs. These results suggest that at the NMJ, the region of high 43DAG concentration encompasses the area of highest intensity labelling for both DRP and dystrophin.
Bewick
G S
GS
Division of Neurobiology, University of Newcastle upon Tyne, U.K.
Nicholson
L V
LV
Young
C
C
Slater
C R
CR
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000378:metabolism; D049030:Dystroglycans; D016189:Dystrophin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D009469:Neuromuscular Junction / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D051381:Rats; D011950:Receptors, Cholinergic / Q000032:analysis* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D016189:Dystrophin; D008562:Membrane Glycoproteins; D010455:Peptides; D011950:Receptors, Cholinergic; D049030:Dystroglycans