[Characterization of anti-mitochondrial antibodies type 2 (anti-M2): comparison of two techniques, western blotting and indirect immunofluorescence].
About 94% of patients with typical features of primary biliary cirrhosis (PBC) have been shown to be anti-M2 positive. Today the relevance of anti-M2 antibodies as a diagnostic marker of PBC is well established. The usual method of detection is by indirect immunofluorescence with cryostat sections of rat organs. In our laboratory we have developed a second identification technique for these antibodies: Western-blotting. To compare immunofluorescence and immunoblotting results, we selected sera from 252 patients: 142 sera from patients with documented PBC, 50 from patients with another hepatic disease, 10 from patients with haematological lupus and 50 from healthy blood donors. We characterized antimitochondrial antibody M2 by the presence of one or more of five antigenic determinants: 65-70 kDa (a), 52-54 kDa (b), 44 kDa (c), 23-26 kDa (d) and 16-20 kDa (e). This technique is especially useful as a backup method intention for identifying a very slight or atypical fluorescence pattern.
Soulié
E
E
Laboratoire central d'immunologie et d'hématologie, hôpital Saint-Antoine, Paris, France.
Moulin
V
V
Fourel
V
V
Dubel
L
L
Kantara-Doukoure
M
M
Johanet
C
C
D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology; D015153:Blotting, Western / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006526:Hepatitis C / Q000276:immunology; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000175:diagnosis* / Q000276:immunology; D008177:Lupus Vulgaris / Q000276:immunology; D008928:Mitochondria / Q000276:immunology*; D012016:Reference Values
D001323:Autoantibodies
Caractérisation des anticorps antimitochondries de type 2 (anti-M2): comparaison de deux techniques, immunotransfert et immunofluorescence indirecte.