Constitutive production of alpha and beta interferons in mutant human cell lines.
Alpha and beta interferons control expression of a selectable marker in the human hypoxanthine phosphoribosyltransferase-negative cell line 2fTGH, in which transcription of gpt is regulated by the upstream region of an interferon-responsive human gene. Selection of mutagenized 2fTGH cells in hypoxanthine-aminopterin-thymidine medium yielded mutants in one recessive (C1) and two dominant (C2 and C3) complementation groups. The mutants constitutively expressed low levels of beta interferon (C1), alpha interferon (C2), or both (C3).
McKendry
R
R
Imperial Cancer Research Fund, London, United Kingdom.
Pellegrini
S
S
Kerr
I M
IM
Stark
G R
GR
D001483:Base Sequence; D002460:Cell Line; D015342:DNA Probes / Q000235:genetics; D005799:Genes, Dominant; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000235:genetics*; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D015342:DNA Probes; D016898:Interferon-alpha; D012333:RNA, Messenger; D016899:Interferon-beta