Area-specific modification of acetylcholinesterase activity following 3-mercaptopropionic acid-induced seizures.
Acetylcholinesterase activity (AChE) was assayed in rat CNS membrane fractions after administration of the convulsant 3-mercaptopropionic acid (150 mg/kg, ip). In comparison with saline-injected controls, total AChE activity decreased 12-20% in striatum and cerebellum during seizure and postseizure but failed to change in cerebral cortex. Specific AChE activity, assayed in the presence of 10(-4) M ethopropazine (a butyrylcholinesterase inhibitor), decreased 15-25% in striatum and cerebellum, increased 20-45% in hippocampus, but remained unchanged in cerebral cortex. Saline injection alone increased AChE activity in striatum (68%) and cerebellum (36%) but failed to modify enzyme activity in hippocampus and cerebral cortex. To conclude, AChE sensitivity to convulsant and saline administration is tissue-specific and not restricted to cholinergic areas.
Girardi
E
E
Instituto de Biología Celular, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Schneider
P
P
Rodriguez De Lores Arnaiz
G
G
D015097:3-Mercaptopropionic Acid; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000503:physiopathology; D002531:Cerebellum / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology; D003342:Corpus Striatum / Q000201:enzymology; D006624:Hippocampus / Q000201:enzymology; D008297:Male; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012640:Seizures / Q000139:chemically induced / Q000201:enzymology*
D015097:3-Mercaptopropionic Acid; D000110:Acetylcholinesterase