Equipment for the large-scale electromanipulation of plant protoplasts.
Electric fields are now used extensively for the genetic manipulation of plant cells through protoplast fusion and direct gene uptake. The cost of commercially available electrofusion and electroporation equipment remains prohibitive for many laboratories. This paper describes an electronic apparatus, suitable for the large-scale electrofusion and electroporation of plant protoplasts that is compatible in both function and cost with commercially available equipment.
Jones
B
B
Department of Life Science, University of Nottingham, UK.
Lynch
P T
PT
Handley
G J
GJ
Malaure
R S
RS
Blackhall
N W
NW
Hammatt
N
N
Power
J B
JB
Cocking
E C
EC
Davey
M R
MR
D001698:Biomedical Engineering; D001709:Biotechnology; D002459:Cell Fusion; D004566:Electrodes; D004574:Electromagnetic Fields; D018274:Electroporation / Q000295:instrumentation*; D004869:Equipment Safety; D059828:Plant Cells; D010944:Plants / Q000235:genetics; D011523:Protoplasts / Q000166:cytology*