[Localization of penicillin G chemoreceptors in cells of Streptomyces sp. R61].
The purpose of the present work was localization of penicillin-binding proteins (PBPs) in the cells of Streptomyces sp. R61 using immunological and autoradiographic methods and electron microscopy. The cells were treated with H3-penicillin G. PBPs of protoplasts were marked with peroxidase labelled IgG to DD-carboxypeptidase of the strain. The results indicate areas privileged in PBPs content at the cytoplasmic membrane and in vesicles located in the periplasmic space. PBPs were visualized in bulges of the protoplasts membrane. In the cells PBPs are present at hyphal tips and in centers at the periphery of cells, which are places of cross wall biosynthesis or branching of hyphae and overproduction of cell wall material.
Filipek
J
J
Zakład Immunopatologii PZH w Warszawie.
Kurzatkowski
W
W
D001665:Binding Sites; D010400:Penicillin G / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000032:analysis*; D011955:Receptors, Drug / Q000032:analysis*; D013302:Streptomyces / Q000737:chemistry*
D011956:Receptors, Cell Surface; D011955:Receptors, Drug; D010400:Penicillin G
Lokalizacja chemoreceptorów penicyliny G w komórkach szczepu Streptomyces sp. R61.