Identification of Chlamydia pneumoniae-specific protein antigens in immunoblots.
The immunoblot patterns of 248 sera, all examined previously by the microimmunofluorescence test (MIF) for species-specific Chlamydia antibodies, were analyzed. Predominant specific antibody activity was directed to the 54 kDa protein of Chlamydia pneumoniae, which was recognized by 93% of sera positive for Chlamydia pneumoniae by MIF but by only 2% of sera positive for Chlamydia trachomatis and negative for Chlamydia pneumoniae and by 3% of sera negative for both Chlamydia pneumoniae and Chlamydia trachomatis. This antigen appears to be specific for Chlamydia pneumoniae. Other Chlamydia pneumoniae-specific protein antigens were recognized far less frequently. Absorption analysis indicated that the 54 kDa protein is located on the surface of the Chlamydia pneumoniae elementary bodies.
Freidank
H M
HM
Institute for Medical Microbiology and Hygiene, University of Freiburg, Germany.
Herr
A S
AS
Jacobs
E
E
D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight
D000942:Antigens, Bacterial; D001426:Bacterial Proteins