title: Double-origin vectors for isolating bidirectional deletions useful in DNA sequence analysis.
abstract: Cosmids containing two compatible origins of replication (oris) have been constructed for isolating bidirectional deletions in cloned DNA. The inserted fragment and the cos site are cut, respectively, with a restriction enzyme and lambda terminase to produce two linear fragments that are circularized to produce two autonomously replicating plasmids. Each plasmid contains a different portion of the insert fused to the cosL or cosR sequence. From a series of such deletion-bearing plasmids, the nucleotide sequence of the insert can be determined in both directions using cosL and cosR primers.
authors.affiliation: Department of Genetics, University of Alberta, Edmonton, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Ahmed
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004261:DNA Replication / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D017384:Sequence Deletion