Oxidation of pyridine nucleotides and depletion of ATP and ADP during calcium- and inorganic phosphate-induced mitochondrial permeability transition.
We have examined the pyridine and adenine nucleotide status during calcium- and inorganic phosphate-induced permeability transition. Calcium- and inorganic phosphate-induced permeability transition is accompanied by the rapid oxidation of pyridine nucleotides and depletion of ATP and ADP with conversion to AMP. The addition of cyclosporin A, a potent inhibitor of the permeability transition prevented the oxidation of pyridine nucleotides and depletion of ATP and ADP.
Savage
M K
MK
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Reed
D J
DJ
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D016572:Cyclosporine / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010539:Permeability; D010710:Phosphates / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000227:Adenine Nucleotides; D010710:Phosphates; D009243:NAD; D009249:NADP; D016572:Cyclosporine; D002118:Calcium