title: Isolation and fluorescence in situ hybridization mapping of 60 cosmid clones on human chromosome 18.
abstract: We have mapped 60 new cosmids on the short and long arms of chromosome 18 either by R- or by DAPI-banding and simultaneous fluorescence in situ hybridization. These markers were isolated from hybrid MS126-21 made from a human-rodent hybrid cell line that retained human chromosome 18. Twenty-two of the cosmid probes map on the short arm, and 31 probes cluster in the distal half of the long arm between bands 18q21.1 and 18q23, while 7 other probes are mapped more proximal to the centromere around bands 18q11.1-q12.3. The technique of fluorescence in situ hybridization has proven to be a very efficient methodology for gene mapping. These 60 probes will be useful in the elucidation of genetic alterations associated with diseases such as tetrasomy 18p syndrome, 18q- syndromes, and colorectal cancer.
authors.affiliation: Department of Human Genetics, National Institute of Genetics, Shizuoka-Ken, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakashima
authors.forename: M
authors.initials: M
authors.lastname: Sakai
authors.forename: R
authors.initials: R
authors.lastname: Inaba
authors.forename: T
authors.initials: T
authors.lastname: Imamura
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D003360:Cosmids / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000648:ultrastructure; D051379:Mice; D012377:Rodentia
chemical_list: D005819:Genetic Markers