title: E-cadherin distribution in interleukin 6-induced cell-cell separation of ductal breast carcinoma cells.
abstract: E-cadherin is expressed in both the ZR-75-1-Tx and the ZR-75-1-Ro sublines of ductal breast carcinoma cells and is concentrated at cell-cell borders as shown by immunocytochemical examination. Free cell borders generally show no or little staining. The localized decrease in E-cadherin expression observed after interleukin 6 (IL-6) treatment of either subline correlates with the increase in free cell borders as IL-6 causes cell-cell separation. As we previously reported, many IL-6-treated ZR-75-1-Tx cells round up and detach from the substratum while ZR-75-1-Ro cells remain adherent and display prominent processes. The results are consistent with the view that E-cadherin expression is not responsible for the marked difference in the IL-6-induced phenotypes in these cell lines, although the localized decrease may play a role in cell-cell separation. ZR-75-1-Tx cells are deficient in desmosomes and show a wider intercellular space than ZR-75-1-Ro cells. Alternative mechanisms involving different aspects of the interlinked cytoskeletal and cell adhesion structures are considered to account for the IL-6-induced antimorphogenetic effect.
authors.affiliation: Laboratory of Cell Physiology and Virology, Rockefeller University, New York, NY 10021.
authors.forename: I
authors.initials: I
authors.lastname: Tamm
authors.forename: I
authors.initials: I
authors.lastname: Cardinale
authors.forename: T
authors.initials: T
authors.lastname: Kikuchi
authors.forename: J G
authors.initials: JG
authors.lastname: Krueger
mesh_terms: D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis*; D018270:Carcinoma, Ductal, Breast / Q000473:pathology* / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D003896:Desmosomes / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000494:pharmacology*; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015820:Cadherins; D015850:Interleukin-6