Induction of tetraploid DNA content by simian virus 40 is dependent on T-antigen function in the G2 phase of the cell cycle.
Previous experiments with the simian virus 40 mutant tsA357R-K (tsA30) demonstrated a T-antigen function that is required for production of cells with a greater-than-G2-phase DNA content. In this study, temperature shift experiments indicated that the temperature-sensitive function of tsA357R-K, which is necessary for entry into the greater-than-G2 phase, is not required in G1 or S but must be supplied in the G2 phase.
Friedrich
T D
TD
Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, New York 12208.
Laffin
J
J
Lehman
J M
JM
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000502:physiology*; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D016195:G2 Phase / Q000235:genetics / Q000502:physiology; D009154:Mutation; D030361:Papillomavirus Infections / Q000235:genetics / Q000378:metabolism; D011123:Polyploidy; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology*; D013696:Temperature; D014412:Tumor Virus Infections / Q000235:genetics / Q000378:metabolism
D000957:Antigens, Viral, Tumor; D004247:DNA