title: A rapid density gradient technique for separating polymorphonuclear granulocytes.
abstract: A gradient separation technique followed by isotonic ammonium chloride haemolysis was compared with two methods for the isolation of polymorphonuclear neutrophils from blood. This technique provided a high yield, excellent purity without lymphocyte and erythrocyte contamination, and made it possible to isolate more than 50 x 10(6) human neutrophils from 15 ml of blood. The polymorphonuclear neutrophils isolated in this way were capable of generating a large amount of reactive oxygen species. This technique for the separation of polymorphonuclear neutrophils is an effective method for in vitro studies.
authors.affiliation: Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Lille, France.
authors.forename: A
authors.initials: A
authors.lastname: Cabanis
authors.forename: B
authors.initials: B
authors.lastname: Gressier
authors.forename: S
authors.initials: S
authors.lastname: Lebegue
authors.forename: C
authors.initials: C
authors.lastname: Brunet
authors.forename: T
authors.initials: T
authors.lastname: Dine
authors.forename: M
authors.initials: M
authors.lastname: Luyckx
authors.forename: M
authors.initials: M
authors.lastname: Cazin
authors.forename: J C
authors.initials: JC
authors.lastname: Cazin
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002499:Centrifugation, Density Gradient / Q000379:methods; D006461:Hemolysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D007958:Leukocyte Count; D008214:Lymphocytes / Q000166:cytology; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D006861:Hydrogen Peroxide