title: Preliminary crystallographic analysis of manganese peroxidase from Phanerochaete chrysosporium.
abstract: Manganese peroxidase from the white rot basidiomycete Phanerochaete chrysosporium has been crystallized in a form suitable for high-resolution X-ray structure determination. Crystals were grown from solutions containing 30% polyethylene glycol 8000, ammonium sulfate and cacodylate buffer at pH 6.5, using macroseeding techniques. A complete data set has been obtained to 2.06 A resolution. The data can be indexed in space group P1 with a = 45.96 A, b = 53.77 A, c = 84.87 A, alpha = 97.01 degrees, beta = 105.72 degrees and gamma = 90.1 degrees, with two peroxidase molecules per asymmetric unit, or in space group C2 with a = 163.23 A, b = 45.97 A, c = 53.72 A and beta = 97.16 degrees, with only one molecule in the assymetric unit. Lignin peroxidase, which shares about 57% sequence identity with manganese peroxidase, was used as a probe for molecular replacement. Unique rotation and translation solutions have been obtained in space groups P1 and C2. The structure has been partially refined in space group C2 to R = 0.22 for data between 10 and 2.06 A.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California Irvine 92717.
authors.forename: M
authors.initials: M
authors.lastname: Sundaramoorthy
authors.forename: K
authors.initials: K
authors.lastname: Kishi
authors.forename: M H
authors.initials: MH
authors.lastname: Gold
authors.forename: T L
authors.initials: TL
authors.lastname: Poulos
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015394:Molecular Structure; D010544:Peroxidases / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase