Acute elevation of intracellular pH translocates protein kinase C activities in rat pinealocytes.
The relationship between elevation of intracellular pH and activation of protein kinase C in rat pinealocytes was investigated in rat pinealocytes. It was found that direct elevation of intracellular pH alone by ammonium chloride or methylamine translocated protein kinase C activities in a dose-dependent manner. This translocation was blocked by EGTA, suggesting a requirement of Ca2+ for the process. However, elevation of intracellular pH by methylamine had no significant effect on intracellular Ca2+ or diacylglycerol content, suggesting the translocation was not due to increased intracellular Ca2+ or diacylglycerol contents. These data suggest that elevation of intracellular pH alone can trigger activation of protein kinase C through a mechanism distinct from increases in diacylglycerol or intracellular Ca2+.
Ho
A K
AK
Department of Physiology, University of Alberta, Edmonton, Canada.
Chik
C L
CL
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D004075:Diglycerides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008744:Methylamines / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010870:Pineal Gland / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004075:Diglycerides; D008744:Methylamines; D000643:Ammonium Chloride; D016718:Arachidonic Acid; C027451:methylamine; D011493:Protein Kinase C; D009638:Norepinephrine