title: Lack of the steroid 15 alpha-hydroxylase gene (Cyp2a-4) in wild mouse strain Mus spretus: rapid evolution of the P450 gene superfamily.
abstract: Steroid 15 alpha-hydroxylase P450(15 alpha) and coumarin 7-hydroxylase P450coh genes Cyp2a-4 and Cyp2a-5 are members of the mouse P450 2A subfamily. Whereas Mus musclus domesticus strains contain both genes, wild mouse strain Mus spretus contains only Cyp2a-5. Evolutionarily, therefore, Cyp2a-5 is ancestral to Cyp2a-4. Moreover, the line to Cyp2a-4 descended as recently as 3 million years ago in an ancestral mouse. This evidence implies a rapid evolution of the P450 gene superfamily.
authors.affiliation: Pharmacogenetics Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
authors.forename: K
authors.initials: K
authors.lastname: Aida
authors.forename: R
authors.initials: R
authors.lastname: Moore
authors.forename: M
authors.initials: M
authors.lastname: Negishi
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D015139:Blotting, Southern; D065637:Cytochrome P-450 CYP2A6; D003577:Cytochrome P-450 Enzyme System / Q000172:deficiency / Q000235:genetics*; D000072467:Cytochrome P450 Family 2; D005796:Genes; D051379:Mice / Q000235:genetics; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000235:genetics; D005810:Multigene Family; D009115:Muridae / Q000235:genetics*; D010802:Phylogeny; D012641:Selection, Genetic; D013045:Species Specificity; D013250:Steroid Hydroxylases / Q000172:deficiency / Q000235:genetics*
chemical_list: D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C460223:Cyp2a4 protein, mouse; D065637:Cytochrome P-450 CYP2A6; D000072467:Cytochrome P450 Family 2; C050356:steroid 15-alpha-hydroxylase