title: Cloning and characterization of a potentially protective chitinase-like recombinant antigen from Wuchereria bancrofti.
abstract: While there is no direct evidence demonstrating the existence of protective immunity to Wuchereria bancrofti infection in humans, the presence of individuals, in populations in areas where infection is endemic, with no clinical evidence of past or current infection despite appreciable exposure to the infective larvae, suggests that protective immunity to filarial parasites may occur naturally. Earlier work indicated that such putatively immune individuals generated antibodies to a 43-kDa antigen from larval extracts of the related filarial parasite Brugia malayi that was recognized by only 8% of the infected population. With rabbit antiserum raised against this 43-kDa antigen, this current study identified a recombinant clone, WbN43, with an insert size of 2.3 kb, from a W. bancrofti genomic expression library. The recombinant fusion protein was differentially recognized by the putatively immune individuals but not by the infected patients. The coding sequence (684 bp) from the 5' end had significant sequence similarity to chitinases from Serratia marcescens, Bacillus circulans, Streptomyces plicatus, and B. malayi. Peptide sequencing of the expressed product also defined a chitinase-like sequence. Molecular characterization indicated WbN43 to be a low-copy-number gene, with expression predominantly in infective larvae and microfilariae but not in adult parasites.
authors.affiliation: Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: N
authors.initials: N
authors.lastname: Raghavan
authors.forename: D O
authors.initials: DO
authors.lastname: Freedman
authors.forename: P C
authors.initials: PC
authors.lastname: Fitzgerald
authors.forename: T R
authors.initials: TR
authors.lastname: Unnasch
authors.forename: E A
authors.initials: EA
authors.lastname: Ottesen
authors.forename: T B
authors.initials: TB
authors.lastname: Nutman
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D015139:Blotting, Southern; D002688:Chitinases / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D014958:Wuchereria bancrofti / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D011994:Recombinant Proteins; D002688:Chitinases