title: Hypermethylation of the human papillomavirus transgenome in transgenic mice.
abstract: We have previously reported an observation that the regulatory sequence of the human papillomavirus (HPV) type 18 genome elicits only basal and unregulated transcriptional activities in transgenic mice [Choo et al., Virology (1992) 188:378-383]. We have now extended the study to other transgenic mice carrying an entire or a subgenomic fragment of HPV-16 and -18. Extremely low levels of HPV transcripts were detected in various organs of the mice only by reverse transcriptase-based polymerase chain reaction. On analysis of the methylation status of the HPV transgenome sequences, hypermethylation was clearly observed, therefore suggesting a possible explanation for low level HPV transgenome transcriptional activity in HPV transgenic mice.
authors.affiliation: Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, R.O.C.
authors.forename: K B
authors.initials: KB
authors.lastname: Choo
authors.forename: L N
authors.initials: LN
authors.lastname: Liew
authors.forename: S J
authors.initials: SJ
authors.lastname: Liew
authors.forename: W T
authors.initials: WT
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001483:Base Sequence; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D008297:Male; D008745:Methylation; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009928:Organ Specificity; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D004248:DNA (Cytosine-5-)-Methyltransferases