Effects of glycosaminoglycans and glycosphingolipids on cytosolic phospholipases A2 from bovine brain.
Two forms of Ca(2+)-independent cytosolic phospholipase A2 activity (110 kDa and 39 kDa) were found in bovine brain. They were separated by Sephadex G-75 column chromatography. The 110 kDa phospholipase A2 was much more active with phosphatidylethanolamine and was not affected by glycosaminoglycans, whereas the 39 kDa phospholipase A2 was much more active with ethanolamine plasmalogen and was markedly inhibited by glycosaminoglycans. Heparan sulphate was the most potent inhibitor, followed by chondroitin sulphate, hyaluronic acid and heparin. Gangliosides, especially the GM3 ganglioside, but not other glycosphingolipids, inhibited the activity of the 39 kDa phospholipase A2 in a dose-dependent manner. The heat-inactivation profiles of the 110 kDa and 39 kDa phospholipases A2 provide further evidence for the differences between these cytosolic enzymes. Interactions between glycosaminoglycans, gangliosides and phospholipases A2 may be involved in the maintenance of membrane function.
Yang
H C
HC
Department of Medical Biochemistry, Ohio State University, Columbus 43210.
Farooqui
A A
AA
Horrocks
L A
LA
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D003600:Cytosol / Q000201:enzymology*; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006028:Glycosphingolipids / Q000494:pharmacology*; D006358:Hot Temperature; D006868:Hydrolysis; D010741:Phospholipases A / Q000037:antagonists & inhibitors* / Q000378:metabolism; D054467:Phospholipases A2
D006025:Glycosaminoglycans; D006028:Glycosphingolipids; D010741:Phospholipases A; D054467:Phospholipases A2