title: [Oligonucleotide directed in vitro mutagenesis].
abstract: In this study, a method for oligonucleotide directed in vitro mutagenesis was described. It includes the following steps: clone of the gene to be mutated into the phagemid pGCI; preparation of single strand template; design and synthesis of mutated oligonucleotides; synthesis of double strand DNA. Using this method, a new BamHI site was originated at the N terminal of Stx-B and BglII site at the C terminal of Stx-B. It is ready to fuse the Stx-B gene to LamB. This method is a very useful tool for genetic engineering.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing.
authors.forename: G
authors.initials: G
authors.lastname: Su
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides; D010957:Plasmids
chemical_list: D009841:Oligonucleotides