title: Thermal inactivation of tryptophan synthase. Stabilization by protein-protein interaction and protein-ligand interaction.
abstract: This study investigates effects of ligands on thermal inactivation of the tryptophan synthase alpha and beta 2 subunits alone and in the alpha 2 beta 2 complex. Addition of pyridoxal phosphate to the apo-beta 2 subunit increases the temperature of one-half inactivation (Ti) from 52 to 77 degrees C. Ligands that promote association of the alpha and holo-beta 2 subunits markedly stabilize the more temperature-labile alpha subunit in the alpha 2 beta 2 complex from irreversible thermal denaturation. The combination of a beta 2 subunit ligand (L-serine) with an alpha subunit ligand (alpha-glycerol 3-phosphate) raises the inactivation temperature (Ti) of the alpha subunit in the holo-alpha 2 beta 2 complex from 54 to 66 degrees C. In contrast, values of Ti for inactivation of the alpha and beta subunits in the holo-alpha 2 beta 2 complex are more similar to respective values for the isolated alpha subunit (50 degrees C) and holo-beta 2 subunit (77 degrees C). Surprisingly, the addition of L-serine results in a larger decrease in the Ti of the beta 2 subunit in the holo-alpha 2 beta 2 complex (78 degrees C-->64 degrees C) than in Ti of the holo-beta 2 subunit alone (77 degrees C-->71 degrees C). The observation that ligands have different effects on the isolated and associated subunits provides evidence that the alpha and beta 2 subunits do not fully dissociate during thermal inactivation of the alpha 2 beta 2 complex at pH 7.8 and at approximately 0.1 ionic strength. Our results demonstrate that linkage between protein-ligand interactions and protein-protein interactions affects the conformational stability of the tryptophan synthase alpha 2 beta 2 complex.
authors.affiliation: Laboratory of Biochemical Pharmacology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: S B
authors.initials: SB
authors.lastname: Ruvinov
authors.forename: E W
authors.initials: EW
authors.lastname: Miles
mesh_terms: D001051:Apoenzymes / Q000037:antagonists & inhibitors / Q000737:chemistry; D001665:Binding Sites; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli; D006358:Hot Temperature; D046911:Macromolecular Substances; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry; D012486:Salmonella typhimurium / Q000201:enzymology*; D013816:Thermodynamics; D014367:Tryptophan Synthase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000737:chemistry*
chemical_list: D001051:Apoenzymes; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D011732:Pyridoxal Phosphate; D014367:Tryptophan Synthase