NGF-induced stabilization of GAP-43 mRNA is mediated by both 3' untranslated region and a segment encoding the carboxy-terminus peptide.
NGF stabilizes cytoplasmic mRNA for GAP-43, a neuronal growth cone protein. A 292 b segment of rat GAP-43 mRNA was determined to mediate this stabilizing effect. Insertion of the 292 b segment into heterologous mRNA was also shown to constitutively destabilize the mRNA in the absence of NGF. This 292 b segment contains the sequences encoding the carboxy-terminus peptide and part of the 3' untranslated region. Neither one of these sequences can fully mediate the NGF-dependent stability control independently from each other. Premature termination of translation before the 292 b segment reduces both the constitutive destabilization by the 292 b segment and the NGF-induced stabilization. These findings indicate that the constitutive destabilizing function of the 292 b segment is dependent on the direct translation of this segment and that NGF suppresses the destabilizing function of this segment.
Nishizawa
K
K
Department of Neurobiology, Faculty of Medicine, University of Tokyo, Japan.
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D004355:Drug Stability; D019922:GAP-43 Protein; D005822:Genetic Vectors; D006207:Half-Life; D008562:Membrane Glycoproteins / Q000235:genetics*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016716:PC12 Cells; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D003609:Dactinomycin