Effect of recombinant IL-1 beta and recombinant gamma interferon on septic acute lung injury in mice.
The effect of recombinant human interleukin 1B (IL-1B) and recombinant human gamma interferon (IFN-g), when given prophylactically, in a mouse model of septic acute lung injury was studied. Mice were treated with various doses of IL-1B and IFN-g for 3 consecutive days prior to administration of lipopolysaccharide of Escherichia coli (1 mg/kg given intraperitoneally). To determine the histologic changes occurring after prophylactic administration of such cytokines, a scoring system was assessed. A significant reduction of edema and neutrophil accumulation into the lungs of mice was observed, especially at doses of 100 U per mouse and 10,000 U per mouse of IL-1B and IFN-g, respectively. Prophylactic administration of IL-1B or IFN-g caused histologic changes, including marked reduction of edema and neutrophil accumulation in the interstitial and alveolar spaces. Combined prophylactic administration of IL-1B and IFN-g provoked a marked decrease of neutrophil accumulation into the lungs, but was not accompanied by significant reduction of edema or hemorrhage. These results provide evidence for the beneficial role of IL-1B and IFN-g in the abnormality of septic acute lung injury by reducing inflammatory lesions.
Torre
D
D
Division of Infectious Diseases, Regional Hospital, Varese, Italy.
Minoja
G
G
Maraggia
D
D
Chiaranda
M
M
Tambini
R
R
Speranza
F
F
Giola
M
M
D000818:Animals; D004926:Escherichia coli; D005260:Female; D007371:Interferon-gamma / Q000008:administration & dosage*; D007375:Interleukin-1 / Q000008:administration & dosage*; D008070:Lipopolysaccharides; D008168:Lung / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000473:pathology; D011654:Pulmonary Edema / Q000150:complications / Q000473:pathology; D011994:Recombinant Proteins; D012128:Respiratory Distress Syndrome / Q000150:complications / Q000473:pathology / Q000517:prevention & control*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D007371:Interferon-gamma