Transcription of ligninase H8 by Phanerochaete chrysosporium under nutrient nitrogen sufficient conditions.
Dot blot analyses showed that more ligninase H8 mRNA was present in ammonium sufficient cultures of Phanerochaete chrysosporium than in ammonium limited cultures. Reverse transcription followed by polymerase chain reaction and DNA sequencing verified that H8 mRNA was present under both conditions. Fast protein liquid chromatography profiles indicated that H8 was present but lacked heme in ammonium sufficient cultures. These data indicated that H8 was transcribed and translated by day 5 under ammonium sufficient conditions, but was inactive due to lack of heme.
Johnston
C G
CG
Biotechnology Center, Utah State University, Logan 84322-4705.
Aust
S D
SD
D000363:Agaricales / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003470:Culture Media; D007438:Introns; D010105:Oxygenases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014158:Transcription, Genetic
D003470:Culture Media; D012333:RNA, Messenger; C086881:ligninase H8; D010544:Peroxidases; D010105:Oxygenases; C044391:ligninase