Effect of sanguinarine on the transport of essential nutrients in an everted gut sac model: role of Na+,K(+)-ATPase.
The effect of the argemone alkaloid, sanguinarine, was studied on the active transport of D-glucose and some of the L-amino acids in everted sacs of the small intestine of rats. Sanguinarine (1.0 mumole) was found to inhibit (61%) the transport of D-glucose, while an alkaloid concentration of 0.1 mumole was ineffective. Both 0.1 and 1.0 mumole of sanguinarine had no effect on the transport of the L-amino acids including aspartic acid, lysine, and tyrosine. Sanguinarine showed a dose dependent inhibition of intestinal and hepatic Na+,K(+)-ATPase in a non-competitive manner. The inhibition of Na+,K(+)-ATPase by sanguinarine may in turn inhibit the active transport of D-glucose which requires a sodium pump.
Tandon
S
S
Dyes and Food Adulterant Toxicology Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
Das
M
M
Khanna
S K
SK
D000470:Alkaloids / Q000493:pharmacokinetics / Q000494:pharmacology*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D053119:Benzophenanthridines; D001692:Biological Transport / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007546:Isoquinolines; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000502:physiology*
D000470:Alkaloids; D000596:Amino Acids; D053119:Benzophenanthridines; D007546:Isoquinolines; C005705:sanguinarine; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose