title: The activity of parafusin is distinct from that of phosphoglucomutase in the unicellular eukaryote Paramecium.
abstract: In this paper we identified the presence of a Paramecium phosphoglucomutase enzymatic activity which is clearly distinct from that of parafusin-the exocytosis-related phosphoglycoprotein. Since the recently cloned parafusin showed homology to rabbit muscle phosphoglucomutase, we have designed a specific peptide parafusin antibody-generated to a region not present in any known sequenced phosphoglucomutases-to distinguish parafusin from the Paramecium phosphoglucomutase. Separation of these two proteins was obtained using liquid chromatography, enzymatic activity assay and immunoblotting analysis with the specific parafusin peptide antibody. Parafusin fractions incorporated [B35S]UDP-Glc but not [35S]Glc-1-P whereas Paramecium phosphoglucomutase fractions incorporated [35S]Glc-1-P but not [B35S]UDP-Glc. This indicates that these two proteins are separate entities exhibiting different properties and most likely distinct functions in the cell.
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: A P
authors.initials: AP
authors.lastname: Andersen
authors.forename: E
authors.initials: E
authors.lastname: Wyroba
authors.forename: M
authors.initials: M
authors.lastname: Reichman
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: B H
authors.initials: BH
authors.lastname: Satir
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D005089:Exocytosis; D006023:Glycoproteins / Q000378:metabolism; D010247:Paramecium / Q000201:enzymology*; D010733:Phosphoglucomutase / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D015800:Protozoan Proteins
chemical_list: D006023:Glycoproteins; D010750:Phosphoproteins; D015800:Protozoan Proteins; C087273:parafusin protein, Paramecium; D010733:Phosphoglucomutase