title: A negative regulatory element upstream from the mouse Adh-1 gene can down-regulate a heterologous promoter.
abstract: Regulation of Adh-1 (encoding mouse alcohol dehydrogenase-A2) gene expression results from the interplay of positive and negative effects. We report here the detection and localization of a negative regulatory element (NRE) to the sequence between -754 and -732 bp relative to the transcription start point (tsp). This element, which we call Adh-1 NRE2, can down-regulate transcription from a strong heterologous promoter (the SV40 promoter+enhancer). Adh-1 NRE2 can function in both orientations and either upstream or downstream from the promoter, and thus has attributes characteristic of a 'silencer'. A protein that binds to the Adh-1 NRE is widespread in mouse tissues.
authors.affiliation: Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianopolis 46202-5122.
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: R E
authors.initials: RE
authors.lastname: Jerome
authors.forename: H J
authors.initials: HJ
authors.lastname: Edenberg
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000818:Animals; D004268:DNA-Binding Proteins; D003851:Deoxyribonucleases; D015536:Down-Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D014018:Tissue Distribution; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; D000426:Alcohol Dehydrogenase; D003851:Deoxyribonucleases