title: The amino-terminal half of the cytoplasmic tail of the thyrotropin receptor is essential for full activities of receptor function.
abstract: We investigated the role of the cytoplasmic tail of the thyrotropin receptor (TSHR) in signal transduction using mutants with truncation at the cytoplasmic tail. Mutant without residues 700-764 completely lost inositol phosphate response to agonists and showed decreased basal cAMP level. Mutant without residues 710-764 showed full cAMP responses but blunted inositol phosphate responses. However, mutant without residues 722-764 retained all signal transduction activities. These findings suggest that the amino-terminal half of the cytoplasmic tail (up to residue 721) is essential for full expression of functional activities.
authors.affiliation: Department of Laboratory Medicine, Kyoto University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kosugi
authors.forename: T
authors.initials: T
authors.lastname: Mori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D015342:DNA Probes; D007295:Inositol Phosphates / Q000378:metabolism; D008961:Models, Structural; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D051381:Rats; D011989:Receptors, Thyrotropin / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology*; D015398:Signal Transduction; D013972:Thyrotropin / Q000378:metabolism; D014162:Transfection
chemical_list: D015342:DNA Probes; D007295:Inositol Phosphates; D011989:Receptors, Thyrotropin; D013972:Thyrotropin; D000242:Cyclic AMP