title: Coagulase deficiency in clinical isolates of Staphylococcus aureus involves both transcriptional and post-transcriptional defects.
abstract: The molecular basis of the non-expression of coagulase was investigated for 14 coagulase-negative isolates of Staphylococcus aureus obtained from different clinical samples. These isolates had typical S. aureus characteristics such as production of clumping factor, DNAase and protein A, but, with one exception, failed to produce detectable amounts of alpha-haemolysin. All 14 strains had DNA homologous to the coagulase gene (coa), but a coa-specific transcript was found in only seven of them. alpha-Haemolysin mRNA was detected in only eight strains without direct correlation to coa-mRNA expression. Thus, coagulase and alpha-haemolysin deficiencies in S. aureus may involve either transcriptional or post-transcriptional alterations although additional regulatory factors may influence the expression of both genes.
authors.affiliation: Département de Recherche en Bactériologie Medicale, Faculté de Médecine Alexis Carrel, Lyon, France.
authors.forename: F
authors.initials: F
authors.lastname: Vandenesch
authors.forename: C
authors.initials: C
authors.lastname: Lebeau
authors.forename: M
authors.initials: M
authors.lastname: Bes
authors.forename: D
authors.initials: D
authors.lastname: McDevitt
authors.forename: T
authors.initials: T
authors.lastname: Greenland
authors.forename: R P
authors.initials: RP
authors.lastname: Novick
authors.forename: J
authors.initials: J
authors.lastname: Etienne
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D003030:Coagulase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000096:biosynthesis; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001427:Bacterial Toxins; D003030:Coagulase; D006460:Hemolysin Proteins; D012333:RNA, Messenger; C021091:staphylococcal alpha-toxin