Biological effects of PEMF (pulsing electromagnetic field): an attempt to modify cell resistance to anticancer agents.
Pulsing Electromagnetic Field (PEMF) effects lead to a modification of the multidrug resistance (MDR) of cells in vitro and in vivo. The murine leukemic doxorubicin-resistant cell line, P388/Dx, subjected to PEMF irradiation in vitro, showed a significant difference in thymidine incorporation when the concentration of doxorubicin reached a level of 1 microgram/mL, which corresponds to the inhibition dose 50 (ID50). The human lymphoblastic leukemia vinblastine-resistant cell line, CEM/VLB100, also showed a significant modification under the same experimental conditions at the in vitro ID50 corresponding to a vinblastine concentration of 100 ng/mL. BDF1 mice transplanted with P388/Dx cells also had an increase in their life span when doxorubicin was injected intraperitoneally in fractionated doses, while being subjected to PEMF irradiation.
Pasquinelli
P
P
C.R.E.S.A.M., Pisa, Italy.
Petrini
M
M
Mattii
L
L
Galimberti
S
S
Saviozzi
M
M
Malvaldi
G
G
D000818:Animals; D003131:Combined Modality Therapy; D004317:Doxorubicin / Q000008:administration & dosage*; D004351:Drug Resistance; D004574:Electromagnetic Fields; D007941:Leukemia P388 / Q000188:drug therapy* / Q000532:radiotherapy*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D014407:Tumor Cells, Cultured; D014747:Vinblastine / Q000008:administration & dosage*
D014747:Vinblastine; D004317:Doxorubicin