Postnatal changes in rhodamine-123 stained mitochondrial populations are sensitive to protein synthesis inhibitors but mimicked in vitro by ATP.
The incubation of term fetus mitochondria with ATP mimicked in vitro the increase in the respiratory control index and in the percentage of the rhodamine-123-low fluorescence population that occurred in vivo immediately after birth, suggesting that both phenomena are closely associated. The administration of streptomycin inhibited the increase in the percentage of the low fluorescence population that occurred immediately after birth, while the administration of cycloheximide even reversed these changes. These results suggest that the in vivo interconversion between mitochondrial forms depends on both cytosolic and mitochondrial protein synthesis.
Almeida
A
A
Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad de Salamanca, Spain.
Lopez-Mediavilla
C
C
Orfao
A
A
Medina
J M
JM
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption; D011247:Pregnancy; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020112:Rhodamine 123; D012235:Rhodamines; D013307:Streptomycin / Q000494:pharmacology
D005456:Fluorescent Dyes; D011500:Protein Synthesis Inhibitors; D012235:Rhodamines; D020112:Rhodamine 123; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003513:Cycloheximide; D013307:Streptomycin