[A new method of immobilizing proteolytic enzymes in polymeric hydrogels].
A novel one-step method for enzyme immobilization in composite gels based on the thermally reversible polymer poly (N-vinyl-caprolactam) was developed. Conditions for entrapment of trypsin and carboxypeptidase B in the gels were optimized. After immobilization, 80 and 90% of the original carboxypeptidase B and trypsin activities, respectively, were found to retain. Both immobilizes enzymes were active in a wider pH and temperature range. The granules with entrapped enzymes were successfully applied to obtain human insulin from recombinant proinsulin.
Markvicheva
E A
EA
Bronin
A S
AS
Kudriavtseva
N E
NE
Kuz'kina
I F
IF
Pashkin
I I
II
Kirsh
Iu E
IuE
Rumsh
L D
LD
Zubov
V P
VP
D002209:Caprolactam / Q000031:analogs & derivatives*; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D004800:Enzymes, Immobilized; D006801:Humans; D006868:Hydrolysis; D011108:Polymers; D011384:Proinsulin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D004800:Enzymes, Immobilized; D011108:Polymers; D011994:Recombinant Proteins; C086861:poly-N-vinylcaprolactam; D002209:Caprolactam; D011384:Proinsulin; D002268:Carboxypeptidases; D010450:Endopeptidases; D043424:Carboxypeptidase B; D014357:Trypsin
Novyĭ metod immobilizatsii proteoliticheskikh fermentov v polimernykh gidrogeliakh.