title: Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization.
abstract: Nonradioactive in situ hybridization with an alpha-satellite DNA probe specific for chromosome 18 was performed on human interphase sperm nuclei to detect the frequency of sperm cells disomic for chromosome 18. A total of 16,127 sperm heads from eight healthy donors, aged 23-57 years, was investigated, and a minimum of 2000 sperm nuclei per proband was analyzed. The disomy rate ranged from 0.25% to 0.5%, with an average of 0.36%. This frequency does not differ significantly from that determined for other chromosomes.
authors.affiliation: Institut für Humangenetik der Universität, Biozentrum, Würzburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Guttenbach
authors.forename: R
authors.initials: R
authors.lastname: Schakowski
authors.forename: M
authors.initials: M
authors.lastname: Schmid
mesh_terms: D000328:Adult; D002467:Cell Nucleus; D002887:Chromosomes, Human, Pair 18; D015342:DNA Probes; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007554:Isotopes; D008297:Male; D008875:Middle Aged; D013077:Sperm Head; D013094:Spermatozoa / Q000648:ultrastructure*
chemical_list: D015342:DNA Probes; D007554:Isotopes