title: Metastatic potential in nude mice of Chinese hamster ovary cells expressing human carcinoembryonic antigen.
abstract: The role of carcinoembryonic antigen (CEA) in metastasis was examined using Chinese hamster ovary (CHO) cells which had been transfected with cDNA encoding CEA. When 2 x 10(6) cells of a clone of CEA-expressing transfectants, designated CHO/CEA, were injected intrasplenically into athymic nude mice, 8 out of 8 mice developed liver metastases. In contrast, a vector-transfectant C5 did not at all form metastasis in the assay (0 of 8). There was no difference in growth rate between CHO/CEA and C5 in vivo as well as in vitro. MoAbs to N-domain of CEA markedly inhibited the liver metastasis of CHO/CEA cells, while a MoAb to domain III of the antigen did not. These findings suggest that CEA may play an important role in hepatic metastasis, and that the N-domain of CEA molecule contributes to the function of CEA in metastasis.
authors.affiliation: Suntory Institute for Biomedical Research, Osaka, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Hashino
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
authors.forename: S
authors.initials: S
authors.lastname: Oikawa
authors.forename: H
authors.initials: H
authors.lastname: Nakazato
authors.forename: T
authors.initials: T
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016466:CHO Cells; D002272:Carcinoembryonic Antigen / Q000276:immunology / Q000502:physiology*; D006224:Cricetinae; D008113:Liver Neoplasms / Q000556:secondary*; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D011994:Recombinant Proteins