title: B cells are essential for murine mammary tumor virus transmission, but not for presentation of endogenous superantigens.
abstract: Murine mammary tumor viruses (MMTVs) are retroviruses that encode superantigens capable of stimulating T cells via superantigen-reactive T cell receptor V beta chains. MMTVs are transmitted to the suckling offspring through milk. Here we show that B cell-deficient mice foster nursed by virus-secreting mice do not transfer infectious MMTVs to their offspring. No MMTV proviruses could be detected in the spleen and mammary tissue of these mice, and no deletion of MMTV superantigen-reactive T cells occurred. By contrast, T cell deletion and positive selection due to endogenous MMTV superantigens occurred in B cell-deficient mice. We conclude that B cells are essential for the completion of the viral life cycle in vivo, but that endogenous MMTV superantigens can be presented by cell types other than B cells.
authors.affiliation: Program of Immunology, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, Massachusetts 02111.
authors.forename: U
authors.initials: U
authors.lastname: Beutner
authors.forename: E
authors.initials: E
authors.lastname: Kraus
authors.forename: D
authors.initials: D
authors.lastname: Kitamura
authors.forename: K
authors.initials: K
authors.lastname: Rajewsky
authors.forename: B T
authors.initials: BT
authors.lastname: Huber
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D001940:Breast / Q000382:microbiology; D004247:DNA; D005260:Female; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011533:Proviruses / Q000302:isolation & purification; D012192:Retroviridae Infections / Q000276:immunology / Q000635:transmission*; D013154:Spleen / Q000382:microbiology; D018089:Superantigens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D018089:Superantigens; D004247:DNA