Interaction of lectins with human platelet serotonin transporter.
Lectin affinity chromatography was used to demonstrate the glycoprotein nature of the serotonin (5-HT) transporter. The human platelet transporter protein was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), labelled with [3H]cyanoimipramine and chromatographed on lectin columns. Wheat germ, gorse seed and lentil lectin-agarose columns specifically retained 5-HT transporter. Peanut and horse gram lectins were ineffective. Concanavalin A showed high non-specific adsorption. Binding of [3H]imipramine to platelet membranes or solubilized 5-HT transporter was not affected by lectins. These data suggest that the lectin interaction with 5-HT transporter is extrinsic to the antidepressant binding site.
Helmeste
D M
DM
Veterans Administration Medical Center, Long Beach, CA 90822.
Chudzik
J
J
Tang
S W
SW
D001665:Binding Sites; D001792:Blood Platelets / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002793:Cholic Acids; D002846:Chromatography, Affinity; D003902:Detergents; D006801:Humans; D007099:Imipramine / Q000378:metabolism; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins
D002352:Carrier Proteins; D002793:Cholic Acids; D003902:Detergents; D037102:Lectins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin; D007099:Imipramine; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate