title: Fate of sperm organelles during early embryogenesis in the rat.
abstract: This report is part of a continuing study in which we employ monoclonal antibodies to membrane domains and internal organelles of rat spermatozoa in order to trace events during maturation, capacitation, fertilization, and early development. In the present study, we have used immunocytochemistry at the light and EM levels to localize one antibody, 5A5, to the fibrous sheath and a second, 3D5, to the outer mitochondrial membrane. Antibody 5A5 does not stain the fibrous sheath of spermatozoa of rodents other than the rat, while 3D5 can be localized to the outer mitochondrial membrane of rat, hamster, and mouse spermatozoa. In order to follow these antibodies during fertilization and early embryogenesis, we developed a method to stain internal components of zygotes and early embryos. Our findings suggests that the fibrous sheath disappears prior to the first cleavage and that mitochondria can be detected up to the 2-cell stage in mouse and the 4-cell stage in rat.
authors.affiliation: Department of Embryology, Sackler School of Medicine, Tel-Aviv University, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Shalgi
authors.forename: A
authors.initials: A
authors.lastname: Magnus
authors.forename: R
authors.initials: R
authors.lastname: Jones
authors.forename: D M
authors.initials: DM
authors.lastname: Phillips
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000378:metabolism; D006224:Cricetinae; D004622:Embryo, Mammalian / Q000276:immunology / Q000648:ultrastructure*; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D007425:Intracellular Membranes / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000276:immunology / Q000648:ultrastructure; D015388:Organelles / Q000276:immunology / Q000648:ultrastructure*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013094:Spermatozoa / Q000276:immunology / Q000648:ultrastructure*; D015053:Zygote / Q000276:immunology / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens