Different immunoreactivities of anti-soluble lactose lectin antisera to tissues from early chick embryos: a histochemical study.
The location of soluble lactose-binding proteins (S-lac lectins) has been studied by immunohistochemical methods during morphogenesis of the chick embryo, when segregation and early differentiation of organ primordia was occurring. Using a panel of polyclonal antisera raised to various purified lectin preparations, we observed striking differences in the antigenic properties of these antisera, indicating that diverse versions of the lectins may be expressed during development. The antisera referred to as anti-L-16, anti-M-16, anti-S-14 and anti-I-14 were respectively raised to native or denatured 16 kDa lectins from adult liver and embryonic muscle and to 14 kDa lectins from embryonic skin and adult intestine. Having determined the optimal immunohistochemical conditions in the preparation of embryo sections (fixation, embedding, sectioning) we show that anti-L-16, anti-S-14 and anti-I-14 mostly bind the lectins expressed at the cell surface, in the extracellular matrix and in some released secretion. As previously shown, anti-L-16 and anti-S-14 are also able to recognize the cytoplasmic form of some migrative lectin-rich cells (primitive streak, neural crest cells, germ cells). Anti-M-16 was bound exclusively to the cytoplasmic form of the 16 kDa lectin in the same cell lines as above and also in some others, such as in the notochord, the myotomal part of the somites, the pharyngeal endoderm and the cardiac muscle. These different antigenic properties may be applied to the accurate mapping of various lectin isoforms and evaluation of the respective contribution of their intra- and extracellular variants during development and differentiation.
Didier
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E
Université de Clermont Blaise Pascal, Laboratoire d'Immunologie G, Aubière, France.
Zalik
S E
SE
Didier
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P
Ledsham
I M
IM
Bayle
D
D
D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000378:metabolism; D002642:Chick Embryo / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D037541:Galectin 4; D006388:Hemagglutinins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D037102:Lectins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D012995:Solubility
D000906:Antibodies; D000941:Antigens; D037541:Galectin 4; D006388:Hemagglutinins; D037102:Lectins