[Dig-DNA probe for etiologic diagnosis in tuberculosis patients].
We have detected the sputa DNA with nonradioactive probe which is labelled to the specific sequence DNA, 31bp-188bp, by dig-11-dUTP. It showed that both the Sensitivity and specificity are satisfactory. The positive rate of the 80 specimens are 56.25%, and the 30 positive specimens of sputum culture are all positive when detected by the Dig-DNA probe.
Wei
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Institute of Virology, Hu bei Medical University Wuhan.
Yang
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Wu
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D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
D015342:DNA Probes; D004269:DNA, Bacterial