title: Detection of herpes simplex virus (HSV) in the saliva from 1,000 oral surgery outpatients by the polymerase chain reaction (PCR) and virus isolation.
abstract: Herpes simplex virus (HSV) was detected by a polymerase chain reaction (PCR) in 47 (4.7%) out of 1,000 saliva samples from the outpatients of an oral and maxillofacial surgery department compared with 27 (2.7%) by conventional virus isolation. There were 20 PCR-positive, culture-negative cases but no culture-positive, PCR-negative cases. Patients younger than 10 years or older than 60 years secreted HSV more frequently than the others. Those with inflammatory diseases showed higher positivity for HSV than those with malignancy, trauma or other complaints. All 27 virus isolates were typed as HSV type 1 and none were resistant to acyclovir, arabinofuranosyl-adenine, iododeoxyuridine or phosphonoacetic acid.
authors.affiliation: First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tateishi
authors.forename: Y
authors.initials: Y
authors.lastname: Toh
authors.forename: H
authors.initials: H
authors.lastname: Minagawa
authors.forename: H
authors.initials: H
authors.lastname: Tashiro
mesh_terms: D000212:Acyclovir / Q000494:pharmacology; D000328:Adult; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D000212:Acyclovir