title: Extensive intracellular translocations of a major protein accompany anoxia in embryos of Artemia franciscana.
abstract: Cells of encysted gastrula embryos of the crustacean Artemia franciscana exhibit extraordinary stability during prolonged anoxia. We find that they contain an abundant protein (referred to as "26-kDa protein") that undergoes translocation to the nucleus during anoxia. The reverse translocation rapidly occurs when anoxic embryos are returned to aerobic conditions. A similar translocation appears to take place in embryos exposed to 42 degrees C aerobic heat shock and prolonged exposure to low temperature (0-2 degrees C), and in diapause embryos. Gel filtration and Western immunoblotting indicate that the 26-kDa protein is translocated to other cellular compartments and may also be associated with a wide variety of "soluble" proteins during anoxia. This protein makes up roughly 15% of the total nonyolk embryo protein and is, by far, most abundant in the encysted embryo stage of the life cycle. The hypothesis is advanced that the 26-kDa protein may play the role of a metabolic regulator and/or a protective molecular chaperone during prolonged anoxia and other forms of stress.
authors.affiliation: Bodega Marine Laboratory, University of California, Davis, Bodega Bay 94923.
authors.forename: J S
authors.initials: JS
authors.lastname: Clegg
authors.forename: S A
authors.initials: SA
authors.lastname: Jackson
authors.forename: A H
authors.initials: AH
authors.lastname: Warner
mesh_terms: D000332:Aerobiosis / Q000502:physiology; D000693:Anaerobiosis / Q000502:physiology; D000818:Animals; D001156:Artemia / Q000196:embryology / Q000378:metabolism*; D001692:Biological Transport; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins