title: Inhibition of phospholipase D blocks activation of fibrinogen-adherent neutrophils by tumor necrosis factor.
abstract: Tumor necrosis factor stimulates fibrinogen-adherent neutrophils to produce a dramatic oxidative burst; the resulting superoxide and other products may contribute to tissue damage in severe infection. Inhibitors of the phospholipase D pathway blocked this activation without affecting cell adherence or nonspecific activation by phorbol myristate acetate. The phospholipase D pathway appears to be involved in the activation of adherent neutrophils by tumor necrosis factor, and this pathway may be a target for modulation of tumor necrosis factor's effects.
authors.affiliation: Department of Medicine, University of Virginia, School of Medicine, Charlottesville 22908.
authors.forename: M O
authors.initials: MO
authors.lastname: Frank
authors.forename: G W
authors.initials: GW
authors.lastname: Sullivan
authors.forename: H T
authors.initials: HT
authors.lastname: Carper
authors.forename: G L
authors.initials: GL
authors.lastname: Mandell
mesh_terms: D002448:Cell Adhesion; D005340:Fibrinogen / Q000502:physiology*; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D005340:Fibrinogen; D010739:Phospholipase D; D013755:Tetradecanoylphorbol Acetate