Establishment and characterization of two new human renal cancer cell lines (DNT-11 and DNT-63) derived from metastases.
Two new human cell lines (DNT-11 and DNT-63) derived from renal cell carcinoma metastases were established and characterized. They have been passaged up to 50 times in vitro for more than 4 years. Each line has an epithelial morphology. Cell cycle time of DNT-11 and DNT-63 was 60 hours and 48 hours, respectively. Both cell lines expressed HLA class I antigens, but were found to be negative for HLA class II. DNT-11 and DNT-63 had a hyperdiploid DNA content. They exhibited anchorage independent growth, and the plating efficiency of DNT-11 and DNT-63 was 0.85% and 1.65%, respectively. These two cell lines may provide a useful tool for studying the biology and immunology of metastatic renal cell carcinoma.
Buszello
H
H
Department of Urology, Heinrich-Heine-University Düsseldorf, Germany.
Ackermann
R
R
D000368:Aged; D000818:Animals; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis; D004171:Diploidy; D005260:Female; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007382:Intermediate Filaments / Q000737:chemistry; D007680:Kidney Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D006680:HLA Antigens