title: Serum and tissue tocopherol in sheep after intramuscular injection and(or) dietary vitamin E supplementation.
abstract: In a 28-d trial, 35 wethers weighing 35 to 60 kg were used to evaluate serum, liver, pancreas, kidney, diaphragm, heart, and neck muscle alpha-tocopherol concentrations after vitamin E administration as i.m. injections and(or) oral supplementation. Treatments (T) were as follows: 1) i.m. DL-alpha-tocopherol(DL-alpha-ol) d 1, 2) i.m. DL-alpha-tocopheryl acetate(DL-alpha-ac) d 1, 3) i.m. DL-alpha-ol d 1, 7, 14, and 21, 4) T 1 and oral supplementation, 5) T 3 and oral supplementation, 6) oral supplementation, and 7) control, no E supplementation. Supplemented wethers received 500 IU via injection and(or) 30 IU/kg of diet (acetate form). In a second 10-d trial, blood was periodically collected from 20 wethers weighing 32 to 47 kg that had been assigned to T as follows: 1) control, 2) i.m. DL-alpha-ol d 1, 3) i.m. DL-alpha-ac d 1, 4) T 2 d 1 and 7, and 5) T 3 d 1 and 7. Results from repeated measures ANOVA in both trials indicated that there were day x T effects (P < .0001) on serum alpha-tocopherol. Pretreatment serum tocopherol concentrations were .58 and .66 microgram/mL for Trials 1 and 2, respectively. In Trial 1, wethers receiving the four alcohol vitamin E forms showed a rapid increase in serum tocopherol between d 1 and 3, followed by a rapid decline to pretreatment concentrations for T 1, whereas T 3, 4, and 5 maintained higher than pretreatment concentrations after the decline.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Science, University of Florida, Gainesville 32611.
authors.forename: C A
authors.initials: CA
authors.lastname: Njeru
authors.forename: L R
authors.initials: LR
authors.lastname: McDowell
authors.forename: N S
authors.initials: NS
authors.lastname: Wilkinson
authors.forename: S B
authors.initials: SB
authors.lastname: Linda
authors.forename: L X
authors.initials: LX
authors.lastname: Rojas
authors.forename: S N
authors.initials: SN
authors.lastname: Williams
mesh_terms: D000042:Absorption; D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D007273:Injections, Intramuscular / Q000662:veterinary; D008099:Liver / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009333:Neck; D010179:Pancreas / Q000378:metabolism; D011897:Random Allocation; D012756:Sheep / Q000378:metabolism*; D014018:Tissue Distribution; D014810:Vitamin E / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D014810:Vitamin E