Characterization of rotavirus VP2 particles.
Rotavirus particles consist of three concentric proteinaceous capsid layers. The innermost capsid (core) is made of VP2. The genomic RNA and the two minor proteins VP1 and VP3 are encapsidated within this layer. Empty rVP2 particles are produced when insect cells are infected with a recombinant baculovirus which contains the bovine Rf rotavirus gene 2 (Labbé et al., 1991, J. Virol. 65, 2946-2952). Analysis of expressed rVP2 particles by SDS-PAGE showed these particles were composed of three major VP2-related proteins, called bands A, B, and C, with apparent molecular weights of 94K, 85K, and 77K, respectively. N-Terminal amino acid sequence analysis of each band showed that band A and band B were blocked, and band C lacked 92 amino acids from the N terminus. Bands B and C were predicted to also lack an approximately 10K peptide fragment from the C terminus. Electron microscopy (EM) showed negatively stained rVP2 particles to be spherical with icosahedral symmetry, 520 +/- 20 A in diameter. Highly concentrated rVP2 particles were converted to unusual forms, including elongated bristly structures, helix-like structures, and sheet-like helix structures. These unusual forms apparently resulted from a structural conversion of individual rVP2 particles. This conversion was reversible both in solution or on a collodion-carbon-coated grid support. The reconstituted rVP2 particles possessed normal morphology and reacted with purified VP6 to form rVP2/6 empty double-layered (previously called single-shelled) virus-like particles with an association constant Ka approximately 10(11) M-1. Native viral core particles lacking RNA were obtained by dialysis of full cores prepared from purified SA11-4F rotavirus double-layered particles against a hypotonic buffer in the presence of EDTA. EM showed both the full and empty native viral cores to be spherical with icosahedral symmetry. Highly concentrated SA11-4F full and empty cores also were converted into elongated and bead-like structures. However, in contrast to rVP2 particles, the conversion of SA11-4F cores was not reversible. These results provide some helpful clues to understanding VP2 functions, the assembly of VP2 particles, the assembly of VP2/6 double-layered particles, and the transport of metabolites inside and outside of the core particle.
Zeng
C Q
CQ
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
Labbé
M
M
Cohen
J
J
Prasad
B V
BV
Chen
D
D
Ramig
R F
RF
Estes
M K
MK
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D016367:Baculoviridae / Q000235:genetics; D002213:Capsid / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins; D002417:Cattle; D003890:Desiccation; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009994:Osmolar Concentration; D011485:Protein Binding; D012401:Rotavirus / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D017421:Sequence Analysis
D000956:Antigens, Viral; D036022:Capsid Proteins; C442545:VP2 protein, Rotavirus; C061332:VP6 protein, Rotavirus