[The isolation and detection of actellic by thin-layer chromatography in the forensic chemical study of cadaveric organs].
A method for analysis of human cadaveric organs for actellik, an organophosphorus insecticide (pyrimiphos methyl), has been developed, consisting in the agent isolation using hexan, separation of extraction substances by extraction redistribution between hexan and dimethylformamide, and subsequent preparative chromatographic isolation in a sorbent layer (KSK silica gel or silicic acid) with chloroform used as solvent. This method permits isolation of 49-52% of actellic added to the liver. A method of purified extract analysis by thin-layer chromatography on silufol plates and KSK silica gel using a complex of chromogenic reagents and selective solvent system has been worked out. The method permits detection of 0.12 to 0.6 mg of actellic in 25 g of organic tissue depending on analysis conditions.
Gorbacheva
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D002102:Cadaver; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D007306:Insecticides / Q000032:analysis / Q000302:isolation & purification*; D007422:Intestines / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D009946:Organothiophosphorus Compounds / Q000032:analysis / Q000302:isolation & purification*; D013270:Stomach / Q000737:chemistry
D007202:Indicators and Reagents; D007306:Insecticides; D009946:Organothiophosphorus Compounds; C014153:pirimiphos methyl
Izolirovanie i obnaruzhenie aktellika metodom tonkosloĭnoĭ khromatografii pri sudebno-khimicheskom issledovanii organov trupa.