Nitric oxide destroys zinc-sulfur clusters inducing zinc release from metallothionein and inhibition of the zinc finger-type yeast transcription activator LAC9.
Nitric oxide, generated from S-nitrosocysteine or applied as gas mediates metal ion release from the Zn2+/Cd(2+)-complexing protein metallothionein via oxidation of SH-groups. Time-dependent S-nitrosylation and subsequent disulfide formation of metallothionein are demonstrated. Furthermore, nitric oxide inhibits DNA binding activity of the yeast transcription factor LAC9 containing a zinc finger like DNA binding domain. These results show that nitric oxide interacts with and destroys zinc-sulfur clusters in proteins.
Kröncke
K D
KD
Medical Department, Heinrich-Heine University, Düsseldorf, Fed. Rep. Germany.
Fehsel
K
K
Schmidt
T
T
Zenke
F T
FT
Dasting
I
I
Wesener
J R
JR
Bettermann
H
H
Breunig
K D
KD
Kolb-Bachofen
V
V
D000818:Animals; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors; D005656:Fungal Proteins / Q000037:antagonists & inhibitors*; D006736:Horses; D066298:In Vitro Techniques; D008668:Metallothionein / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000633:toxicity*; D010084:Oxidation-Reduction; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D014157:Transcription Factors / Q000037:antagonists & inhibitors; D015032:Zinc / Q000378:metabolism*; D016335:Zinc Fingers / Q000187:drug effects
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C058596:LAC9 protein, Kluyveromyces lactis; D013438:Sulfhydryl Compounds; D014157:Transcription Factors; D009569:Nitric Oxide; D013455:Sulfur; D008668:Metallothionein; D015032:Zinc