Interaction of poliovirus with its cell surface binding site.
The interaction of poliovirus with its cellular binding sites was characterized by using a receptor-excess silicon oil partition assay. Poliovirus type 1 Mahoney [PV1(M)] binding to HeLa cells fits a theoretical simple bimolecular noncooperative binding curve with an equilibrium dissociation constant (Kd) of 4.3 x 10(7) cells.ml-1 at 4 degrees, or 2.1 x 10(-10) M, assuming 3000 virus binding sites/cell. The association rate of complex formation was measured to be 3.6 x 10(-9) ml.cell-1.min-1 (7.2 x 10(8) M-1.min-1) and the dissociation rate calculated to be 1.5 x 10(-1) min-1, giving the complex a half-life of 4.5 min. The equilibrium dissociation constant, association rate, and dissociation rate were also measured for the binding of the attenuated poliovirus type 3 Sabin strain [PV3(S)] to HeLa cells. PV3(S) bound HeLa cells with a Kd of 3.3 x 10(7) cells.ml-1 (1.6 x 10(-10) M), an association rate of 4.1 x 10(-9) ml.cell-1.min-1 (8.2 x 10(8) M-1.min-1), and a dissociation rate calculated to be 1.4 x 10(-1) min-1, giving the complex a half-life of 5.1 min. Thus the virulent and avirulent strains of poliovirus bind HeLa cells with nearly identical binding constants and rate constants. Equilibrium binding constants for PV1(M) to various other cell types varied from a high affinity of 4.1 x 10(6) cells.ml-1 for JA-1 cells to a low affinity of 7 x 10(7) cells.ml-1 for NGP cells.(ABSTRACT TRUNCATED AT 250 WORDS)
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JA
Department of Microbiology, SUNY at Stony Brook 11794-8621.
Witherell
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Bernhardt
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Wimmer
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D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008565:Membrane Proteins; D017955:Poliovirus / Q000378:metabolism*; D011233:Precipitin Tests; D011991:Receptors, Virus / Q000032:analysis / Q000378:metabolism*; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; D011991:Receptors, Virus; C066100:poliovirus receptor