title: Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts.
abstract: The co-cultures of five different human tumor cell lines with human normal fibroblasts significantly stimulated the production of tissue inhibitors of metalloproteinases-1 (TIMP-1) when compared to cultures of individual cells. In the co-culture of T24 human urinary bladder carcinoma cells and CCD18 human fibroblasts, production of both TIMP-1 and metalloproteinases was stimulated, and the stimulatory effects were dependent on the cellular ratio between the fibroblasts and carcinoma cells. On day 6 of culture, collagenase and stromelysin were stimulated at a ratio of CCD18 fibroblasts to T24 cells of 1:0.1, while the maximum TIMP-1 production occurred at a ratio of 1:1. Thus, the cellular ratio in the interaction of carcinoma cells with host fibroblasts affects the production of TIMP-1 and metalloproteinases and hence modulates the balance between them.
authors.affiliation: Department of Pathology, Miyazaki Medical College, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nabeshima
authors.forename: J
authors.initials: J
authors.lastname: Kishi
authors.forename: T
authors.initials: T
authors.lastname: Kurogi
authors.forename: N
authors.initials: N
authors.lastname: Komada
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: M
authors.initials: M
authors.lastname: Koono
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media; D004790:Enzyme Induction; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism*; D019714:Tissue Inhibitor of Metalloproteinases; D014407:Tumor Cells, Cultured / Q000201:enzymology*
chemical_list: D003470:Culture Media; D006023:Glycoproteins; D019714:Tissue Inhibitor of Metalloproteinases; D008666:Metalloendopeptidases