Sequence analysis of putative E3, pVIII, and fiber genomic regions of a porcine adenovirus.
The complete nucleotide sequence of the putative early transcriptional region 3 (E3), plus the hexon-associated polypeptide VIII (pVIII) gene, and the N-terminus of the fiber protein gene of a porcine adenovirus isolate, NADC-1, was determined. The E3 region of NADC-1 was found to be 1879 bp and located between 80 and 85.8 map units. Eight open reading frames (ORFs) and three polyadenylation signals were identified in the r strand. The amino acid sequences predicted to be encoded by ORFs 1 and 8 were compared to the amino acid sequences of human adenovirus type 2 (Ad2) pVIII and fiber protein and found to be 60% and 55% similar, respectively. The amino acid sequence predicted to be encoded by ORF 4 was compared to the human Ad5 14.7 kDa protein and the C-terminus of the amino acid sequence predicted to be encoded by ORF 11 of the bovine Ad3 E3 region and found to be 36% and 41% similar, respectively. A potential signal sequence was identified in ORF 5.
Kleiboeker
S B
SB
Virology Swine Research Unit, National Animal Disease Center, USDA, Ames, IA 50010.
D000256:Adenoviridae / Q000235:genetics*; D017503:Adenovirus E3 Proteins / Q000235:genetics*; D000260:Adenoviruses, Human / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine
D017503:Adenovirus E3 Proteins; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus