title: Effects of Pseudostellaria heterophylla on proliferation and differentiation of murine bone marrow cells.
abstract: The incorporation of tritiated thymidine into the DNA of murine bone marrow cells could be stimulated by fraction PH-I Ba separated from the roots of Pseudostellaria heterophylla in a dose-dependent manner in vitro. Moreover, PH-I Ba could induce the differentiation of murine bone marrow cells from pluripotent haemopoietic stem cells into macrophages-like cells in vitro. Autocrine or paracrine stimulation of granulocyte-macrophage colony stimulating factor was likely to underly the induction of differentiation. Therefore, PH-I Ba was proved to be an immunostimulating agent for mouse marrow hematopoiesis and was found to be a polysaccharide. The sugar components were analyzed by Gas Liquid Chromatography of their alditol acetates.
authors.affiliation: Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories.
authors.forename: C K
authors.initials: CK
authors.lastname: Wong
authors.forename: K N
authors.initials: KN
authors.lastname: Leung
authors.forename: K P
authors.initials: KP
authors.lastname: Fung
authors.forename: Y M
authors.initials: YM
authors.lastname: Choy
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002849:Chromatography, Gas; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D051379:Mice
chemical_list: D004365:Drugs, Chinese Herbal; D016178:Granulocyte-Macrophage Colony-Stimulating Factor