In vitro development of mouse marrow stromal cell cultures: distinct effect on growth and differentiation of marrow progenitor cells.
Mouse marrow stromal cells (MSC) were cultured up to 7 months in the absence of hydrocortisone. The efficiency of stromal layer in supporting the growth/differentiation of marrow progenitor cells (MPC) was studied. Senescent and quiescent MSC stimulated rapidly both stromal progenitor cells and hematopoietic progenitor cells. A transient production of two types of small blast cells resembling the stem cells described by Terstappen et al. (1991) was observed. In the subcultures obtained after limited dilution of the blast cells, formation of the stroma preceded production of hematopoietic cells.
Milchev
G I
GI
Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.
D000818:Animals; D001854:Bone Marrow Cells; D002452:Cell Count; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D017154:Stromal Cells / Q000166:cytology
D003470:Culture Media