title: Prostaglandin F2 alpha receptor is coupled to Gq in cDNA-transfected Chinese hamster ovary cells.
abstract: We examined intracellular signal transduction of prostaglandin F2 alpha (PGF2 alpha) receptors by transfection and stable expression of the receptor cDNA in Chinese hamster ovary (CHO-PGF2 alpha) cells. The binding to the membrane was specific for PGF2 alpha, and the Scatchard plot analysis showed a single high-affinity binding site (Kd = 25.2 nM). PGF2 alpha gradually elevated intracellular Ca2+ and stimulated phosphoinositide metabolism in CHO-PGF2 alpha cells. In whole-cell clamp recordings, PGF2 alpha induced an outward current in the presence of external Ca2+, but it induced a long-lasting inward Ca2+ current in a Na(+)-free solution containing K+ channel blockers. Gq alpha antibody applied intracellularly blocked both outward and inward currents induced by 1 microM PGF2 alpha. These results demonstrate that the PGF2 alpha receptor is coupled to phosphoinositide metabolism in CHO-PGF2 alpha cells via Gq.
authors.affiliation: Department of Cell Biology, Osaka Bioscience Institute, Suita, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Sakamoto
authors.forename: N
authors.initials: N
authors.lastname: Mochizuki-Oda
authors.forename: T
authors.initials: T
authors.lastname: Ezashi
authors.forename: K
authors.initials: K
authors.lastname: Miwa
authors.forename: E
authors.initials: E
authors.lastname: Okuda-Ashitaka
authors.forename: V I
authors.initials: VI
authors.lastname: Shevchenko
authors.forename: Y
authors.initials: Y
authors.lastname: Kiso
authors.forename: O
authors.initials: O
authors.lastname: Hayaishi
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics*; D015237:Dinoprost / Q000378:metabolism; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D007700:Kinetics; D003341:Luteolysis / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; D015237:Dinoprost; D019204:GTP-Binding Proteins; D002118:Calcium