A Ki-1-positive cell line expressing Epstein-Barr virus antigens, established from a child with Ki-1-positive lymphoma.
We report here the establishment of a CD30 (Ki-1) antigen-positive cell line 'Ki-JK' from a child with anaplastic large cell lymphoma. We characterized this cell line and show that: (i) Ki-JK cells do not grow in soft agar but infrequently produce a tumor or suppressed growth when injected into nude mice; (ii) Ki-JK cells expressed Epstein-Barr virus (EBV) antigens, EBV-associated nuclear antigen-2 and latent membrane protein; (iii) Ki-JK cells are labelled by in situ hybridization using an RNA probe derived from the BamHI W fragment of EBV, and (iv) polymerase chain reaction demonstrates the presence of an EBV BamHI W sequence in DNA of lymphoma and Ki-JK cells. These results suggest an etiological role for EBV in the development of some cases of Ki-1-positive lymphomas.
Shimakage
M
M
Clinical Research Institute, Osaka National Hospital, Japan.
Dezawa
T
T
Tamura
S
S
Tabata
T
T
Aoyagi
N
N
Koike
M
M
Inoue
H
H
Yutsudo
M
M
Hakura
A
A
Ikegami
N
N
D000293:Adolescent; D015703:Antigens, CD / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis*; D001483:Base Sequence; D002460:Cell Line / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007621:Karyotyping; D017730:Ki-1 Antigen / Q000032:analysis*; D017728:Lymphoma, Large-Cell, Anaplastic / Q000209:etiology / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data
D015703:Antigens, CD; D000956:Antigens, Viral; D017730:Ki-1 Antigen