Intracellular dynamics of ricin followed by fluorescence microscopy on living cells reveals a rapid accumulation of the dimeric toxin in the Golgi apparatus.
The intracellular dynamics of fluorescent conjugates of the toxic lectin ricin was followed by video fluorescence microscopy on living CHO cells, demonstrating that the ricin heterodimer and its isolated B chain, after binding to the plasma membrane receptors, migrate to and accumulate in the Golgi apparatus following internalization. A ricin derivative labelled with fluorescein on the A chain and rhodamine on the B chain did not display significant splitting of the A-B heterodimer during translocation of the toxin to the Golgi; this novel finding provides support for the hypothesis that further processing of ricin takes place in this cellular compartment.
Lendaro
E
E
Dipartimento di Scienze Biochimiche, C.N.R., Università di Roma La Sapienza, Italy.
Ippoliti
R
R
Bellelli
A
A
Brunori
M
M
Evangelista
V
V
Guidarini
D
D
Benedetti
P A
PA
D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D019793:Fluorescein; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006056:Golgi Apparatus / Q000378:metabolism*; D046911:Macromolecular Substances; D008856:Microscopy, Fluorescence; D012235:Rhodamines; D012276:Ricin / Q000378:metabolism*; D014741:Video Recording
D005452:Fluoresceins; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D012235:Rhodamines; C009434:tetramethylrhodamine isothiocyanate; D012276:Ricin; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein