[Rapid karyotyping of mammalian cells].
The use of "pipette" method ensures rapid preparation of standardized whole metaphase spreads. Experiments with human, murine, Chinese hamster, American mink, green African monkey, dog, and vole cells demonstrated that G-banded whole metaphase spreads can be obtained in less than two hours after the beginning of work with cell or tissue culture. Due to that, it became possible to start karyotyping of animal tissue explants, as well as fetal cells present in human amniotic fluid, on day 3 to 4 after their receiving.
Rubtsov
N B
NB
Protopopov
A I
AI
Matveeva
V G
VG
Rubtsova
N V
NV
Nesterova
T B
TB
Zakiian
S M
SM
D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques; D006801:Humans; D006824:Hybridization, Genetic; D007621:Karyotyping; D008322:Mammals / Q000235:genetics*; D008677:Metaphase / Q000235:genetics; D013997:Time Factors
Bystroe kariotipirovanie kletok mlekopitaiushchikh.