Long-term exercise of young and adult female rats: effect on femoral neck biomechanical competence and bone structure.
The present study was designed to examine the effect of exercise on femoral neck bone structure and strength in female rats after 4 and 10 months of exercise. Female Fischer rats aged 2 months were exercised for 4 h a day, 5 days a week on a motor-driven treadmill the speed of which was gradually increased until a daily distance of 2 km was reached. The training level was maintained for 4 months (n = 15) and 10 months (n = 15). Sedentary age-matched rats served as controls (n = 30). At death the proximal third of both femora was obtained from each rat. The left femoral neck was tested in a materials-testing machine, and the right was used for either trabecular bone mass measurement (BV/TV) or scanning electron microscopy (SEM). Biomechanical testing revealed a significant training-induced increase in femoral neck bone strength after 4 months of exercise, which although not accelerated was preserved after 10 months of exercise. Histologic investigation revealed a significant training-induced increase in BV/TV, accounted for by a significantly smaller proportion of marrow space. No difference in cortical area was found. Both histology and SEM revealed a tendency to an earlier closure of the growth line in the exercised animals. However, the exercised animals aged 6 months had a significantly increased total femoral length compared with the sedentary rats. No difference was found in total femoral length between sedentary and exercised rats aged 12 months. On the basis of this study, it is concluded that exercise has a positive effect on femoral neck bone strength.(ABSTRACT TRUNCATED AT 250 WORDS)
Søgaard
C H
CH
Department of Connective Tissue Biology, Institute of Anatomy, University of Aarhus, Denmark.
Danielsen
C C
CC
Thorling
E B
EB
Mosekilde
L
L
D000375:Aging / Q000502:physiology; D000818:Animals; D001696:Biomechanical Phenomena; D001835:Body Weight / Q000502:physiology; D015519:Bone Density; D005260:Female; D005269:Femur / Q000033:anatomy & histology; D005272:Femur Neck / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010805:Physical Conditioning, Animal; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344