[Colored biomicrospheres for studies of cochlear microcirculation].
For many years in our laboratory, toads red blood cells have been used as biomicrospheres for study of cochlear microcirculation. In the present experiments the cochlear blood flows (CBF) was twice determined in the same animal by using colored biomicrospheres. The red blood cells were stained separately with eosin and azure blue. The biomicrospheres of two different colors were injected intracardially in the same guinea pig at two different times with an interval of 4 min. The CBF were detected by using a laser Doppler system simultaneously. The finding showed that color of red and blue biomicrospheres were contrast sharply, they were all distinguished easily from the surrounding tissue. The adequate number of microspheres injected each time was 9 x 10(6)/ml. The results of CBF were not different between the first and second injection.
Wang
J B
JB
Department of otorhinolaryngology, Union Hospital, Tong-Ji Medical University, Wuhan.
D000818:Animals; D003051:Cochlea / Q000098:blood supply*; D005260:Female; D006168:Guinea Pigs; D017078:Laser-Doppler Flowmetry; D008297:Male; D008833:Microcirculation / Q000502:physiology; D008863:Microspheres