[Fluticasone propionate reduced the production of GM-CSF, IL-6 and IL-8 generated from cultured nasal epithelial cells].
Accumulation of mast cells and eosinophils in the nasal epithelial layer occurs in nasal allergic reactions and nasal polyps. We have already demonstrated that fluticasone propionate (FP) inhibits the accumulation of mast cells and eosinophils locally, and also improves the nasal symptoms of patients with allergic rhinitis. We hypothesized that cytokines generated from nasal epithelial cells possibly contribute to the accumulation of cells and eosinophils in the nasal epithelial layer. In this experiment we examined the inhibitory effect of FP on the production of GM-CSF, IL-6 and IL-8 by culturing of nasal epithelial cells in vitro. Our results show that FP significantly reduces the level of GM-CSF, IL-6 and IL-8 in the supernatant of culture media of nasal epithelial cells for a period of 6 days. In addition, preincubation of nasal epithelial cells with FP for 6 days causes a significant reduction of GM-CSF levels in the supernatant of culture-media of cultured nasal epithelial cells during the subsequent period of 6 days without FP. These results provide evidence that FP inhibits the accumulation of mast cells and eosinophils in the mucoepithelial layer of the nasal membrane.
Ohnishi
M
M
Department of Otorhinolaryngology, Nippon Medical School.
Takizawa
R
R
Ohkubo
K
K
Yokosima
K
K
Okuda
M
M
Yagi
T
T
D000287:Administration, Topical; D000328:Adult; D000730:Androstadienes / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002452:Cell Count; D002478:Cells, Cultured; D003864:Depression, Chemical; D004804:Eosinophils; D004848:Epithelium / Q000378:metabolism; D005260:Female; D000068298:Fluticasone; D005938:Glucocorticoids; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D008297:Male; D008407:Mast Cells; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000378:metabolism / Q000473:pathology
D000730:Androstadienes; D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D015850:Interleukin-6; D016209:Interleukin-8; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000068298:Fluticasone