[Hypocrellin B photosensitization effected a change of cytoplasmic free calcium concentration in ascitic hepatoma cells].
The Hypocrellin B (HB) is a new photosensitizer. HB-induced photodynamic changes of cytoplasmic free Ca2+ in ascitic hepatoma (HA) cells were observed using fluorescent calcium indicator Quin 2/AM. It was found that the increase in HB concentrations and exposure time followed by the increase in cytoplasmic free Ca2+. The singlet oxygen and hydroxyl radical generated by HB photosensitization were examined. The lipid peroxidation can be protected by Vit E; the relationship between the plasmic membrane depolarization and cytoplasmic free Ca2+ concentration by HB photodynamic action was investigated.
Yue
J C
JC
Institute of Biophysics, Academia Sinica, Beijing.
Pang
S Z
SZ
Zhang
L
L
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011809:Quinones / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology
D017319:Photosensitizing Agents; D011809:Quinones; D014810:Vitamin E; D010569:Perylene; D002118:Calcium; C064522:hypocrellin B