Effect of some phytogenic agents and synthetic compounds on complement cascade-mediated hemolysis.
A variety of anti-inflammatory compounds obtained from various medicinal plants (phytogenic) as well as some synthetic compounds were tested on the complement cascade in vitro. Bovine erythrocytes were treated with rabbit antibovine red blood cell antibody. Rabbit plasma was diluted with veronal buffer and mixed with erythrocytes in the presence or absence of drugs and incubated. Erythrocytes were pelleted and the absorbance of the supernatant at 412 nm determined. Drugs could be grouped into three categories; (a) those with no effect whatsoever; (b) those which produced definite enhancement of the release of hemoglobin by the complement cascade, and (c) prednisolone, which inhibited the release of hemoglobin by the complement cascade. We suggest that prednisolone and the drugs which had no effect on the complement cascade are safer to use as anti-inflammatory agents, while drugs enhancing the complement cascade may have potential adverse properties.
Rohde
B H
BH
Department of Medical Pharmacology and Toxicology, Texas A & M University College of Medicine, College Station 77843-1114.
Okawara
T
T
Varma
R S
RS
Chiou
G C
GC
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D015941:Complement Hemolytic Activity Assay; D003165:Complement System Proteins / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D006461:Hemolysis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal; D011239:Prednisolone / Q000494:pharmacology; D011817:Rabbits
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D003165:Complement System Proteins; D011239:Prednisolone