Generalized and mobilizable positive-selection cloning vectors.
We have modified the positive-selection cloning vector pUN121 to expand the numbers of unique cloning sites by insertion of a multiple cloning site into the lambda cI gene without disrupting its repressor function, resulting in plasmid pSKM10. Plasmid pSKM10 has seven restriction enzyme sites suitable for general cloning purposes. A mobilizable version (pSKM11) of pSKM10 was constructed by insertion of the IncP mob sequence which permitted mobilization of the plasmid into a wide variety of Gram- bacteria.
Mongkolsuk
S
S
Laboratory of Biotechnology, Chulabhorn Research Institute, Lak si, Bangkok, Thailand.
Rabibhadana
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S
Vattanaviboon
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P
Loprasert
S
S
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004251:DNA Transposable Elements; D004279:DNA, Viral; D005814:Genes, Viral; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D010957:Plasmids / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics
D004251:DNA Transposable Elements; D004279:DNA, Viral; D012097:Repressor Proteins