Oxygenation of unsaturated fatty acids by hepatic microsomes of musk shrew (Suncus murinus).
The cytochrome P-450-dependent monooxygenase in musk shrew (suncus; Suncus murinus) liver microsomes metabolized unsaturated fatty acids (oleic, linoleic, alpha-linolenic and arachidonic acids) to a variety of oxygenated products. alpha-Linolenic acid was the most active substrate. The oxygenation activity increased with an increase in the number of cis double-bonds in the C18 fatty acids. This suggests that the introduction of cis double-bonds in C18 fatty acids is important for the binding of cytochrome P-450 in suncus liver microsomes. Regioselectivity of arachidonic acid oxygenation was observed in suncus liver microsomes; rat liver microsomal cytochrome P-450 generated epoxyeicosatrienoic acids and dihydroxyeicosatrienoic acid as major products while the cytochrome P-450-dependent monooxygenase in suncus liver microsomes yielded omega- and (omega-1)-hydroxyarachidonic acids as major reaction products.
Miura
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Y
Department of Legal Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Oda
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S
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism; D012788:Shrews / Q000378:metabolism*; D013379:Substrate Specificity
D005231:Fatty Acids, Unsaturated; D016718:Arachidonic Acid; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases