A method for the assay of hydrolytic enzymes using dynamic light scattering.
Dynamic light-scattering techniques have been successfully used for the assay of several hydrolytic enzymes. The enzymes were assayed using substrate-coated colloidal particles. Hydrolysis of the substrate coat causes destabilization of the particles followed by particle aggregation. The rate of particle aggregation can be related to the initial concentration of added enzyme.
Weetall
H H
HH
National Institute of Standards and Technology, Biotechnology Division Gaithersburg, MD 20899.
Gaigalas
A K
AK
D003102:Colloids; D004247:DNA / Q000737:chemistry; D003851:Deoxyribonucleases / Q000032:analysis; D005087:Glucan 1,4-alpha-Glucosidase / Q000032:analysis; D006867:Hydrolases / Q000032:analysis*; D006868:Hydrolysis; D008027:Light; D010206:Papain / Q000032:analysis; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine; D013213:Starch / Q000737:chemistry; D014357:Trypsin / Q000032:analysis; D000516:alpha-Amylases / Q000032:analysis
D003102:Colloids; D012710:Serum Albumin, Bovine; D013213:Starch; D004247:DNA; D006867:Hydrolases; D003851:Deoxyribonucleases; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase; D014357:Trypsin; D010206:Papain