title: Mutation detection by denaturing gradient gel electrophoresis (DGGE).
abstract: The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically amplified DNA. The technique is based on the migration of double-stranded DNA molecules through polyacrylamide gels containing linearly increasing concentrations of a denaturing agent. In this review DGGE and the several modifications of the original protocol are presented. Moreover, its applications in human molecular genetics are summarized together with a preliminary comparison with other mutation detection technologies such as chemical cleavage, RNase protection, and single-strand conformation polymorphism.
authors.affiliation: MGC-Department of Human Genetics, Sylvius Laboratorium, Leiden, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Fodde
authors.forename: M
authors.initials: M
authors.lastname: Losekoot
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004252:DNA Mutational Analysis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D017491:Genes, APC; D030342:Genetic Diseases, Inborn / Q000235:genetics; D005914:Globins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D001617:beta-Glucosidase / Q000235:genetics
chemical_list: D005914:Globins; D004247:DNA; D001617:beta-Glucosidase