title: Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements.
abstract: In transgenic tobacco plants the regulatory region of the Agrobacterium genes involved in mannopine synthesis (pmas) directs expression in vascular tissues and is induced upon wounding. To identify cis-acting sequences required for the cell-type-specific and wound-inducible expression of this regulatory region, the expression pattern of chimeric genes in which 5' upstream deletions of the pmas 1' promoter direct the expression of the beta-glucuronidase coding sequence, was analyzed in transgenic tobacco plants. It was found that the pmas1' promoter is specifically expressed in phloem cells and that this tissue-specific pattern of expression is the result of the interaction of at least two negative regulatory elements with a positive element that directs expression in many cell types. It was also found that wound induction is mediated by two different mechanisms, one of them involving a dramatic change in tissue-specific expression.
authors.affiliation: Departamento de Ingeniería Genética de Plantas, Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Guanajuato, México.
authors.forename: A
authors.initials: A
authors.lastname: Guevara-García
authors.forename: G
authors.initials: G
authors.lastname: Mosqueda-Cano
authors.forename: G
authors.initials: G
authors.lastname: Argüello-Astorga
authors.forename: J
authors.initials: J
authors.lastname: Simpson
authors.forename: L
authors.initials: L
authors.lastname: Herrera-Estrella
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000472:pathogenicity; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D017343:Genes, Plant; D005809:Genes, Regulator; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D014018:Tissue Distribution; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology
chemical_list: D009687:Nuclear Proteins; D010940:Plant Proteins; D004247:DNA; D006836:Hydro-Lyases; C070395:mannopine synthase