title: Three-way differentiation of Chinese hamster ovary chromosomes by immunoperoxidase technique using a monoclonal anti-bromodeoxyuridine antibody.
abstract: A new permanent staining procedure for sister chromatid differentiation (SCD) in cultured Chinese hamster ovary cells has been established by combining the three-way differentiation in third mitosis (M3) chromosomes and the immunoperoxidase reaction developed with 3,3'-diaminobenzidine using a monoclonal anti-bromodeoxyuridine (BrdU) antibody. This procedure allows SCD at very low BrdU concentrations, and the evaluation of the sister chromatid exchange frequencies on a per cell-cycle basis.
authors.affiliation: Department of Cell Biology, University of Seville, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Piñero
authors.forename: T
authors.initials: T
authors.lastname: Ortiz
authors.forename: F
authors.initials: F
authors.lastname: Cortés
mesh_terms: D015100:3,3'-Diaminobenzidine; D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D016466:CHO Cells; D002842:Chromatids / Q000648:ultrastructure; D006224:Cricetinae; D004277:DNA, Single-Stranded / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D008938:Mitosis; D012854:Sister Chromatid Exchange
chemical_list: D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D015100:3,3'-Diaminobenzidine; D001973:Bromodeoxyuridine