Inhibition of type 5 adenovirus infectivity by periodate oxidation.
Periodate oxidation of purified type 5 Adenovirus (Ad5) led to a mean loss of infectivity of 6.84 logs. There were no significant differences in adsorption and penetration between oxidized and mock-oxidized virus. However, after infection with oxidized virus, no synthesis of viral structural proteins could be detected and a 78.5% inhibition of viral DNA synthesis was observed. Labelling experiments performed by treating oxidized and mock-oxidized virus with tritiated sodium borohydride revealed that the fiber glycoprotein was one of the proteins labelled in oxidized virus whereas no labelled proteins were detected in non oxidized virus. In addition, it was found that one mol of formaldehyde generated during oxidation of sugar residues was bound per 500 base pairs in oxidized virus. One consequence of this in situ generation of formaldehyde is the formation of DNA-protein crosslinks. The DNA so crosslinked showed different patterns of restriction fragments with endonucleases such as Hpa I, Hind III and Kpn I but not with Xho I.
Ogier
G
G
Laboratoire d'Immunochimie Inserm, Faculté de Médecine Lyon Sud, Université Claude Bernard Lyon I, Oullins, France.
Michal
Y
Y
Thomas
V
V
Quash
G
G
Rodwell
J D
JD
D000260:Adenoviruses, Human / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D000998:Antiviral Agents / Q000633:toxicity*; D003432:Cross-Linking Reagents; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000633:toxicity*; D015183:Restriction Mapping; D013936:Thymidine / Q000378:metabolism; D015678:Viral Structural Proteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism
D000998:Antiviral Agents; D003432:Cross-Linking Reagents; D004279:DNA, Viral; D015678:Viral Structural Proteins; D010504:Periodic Acid; C009288:metaperiodate; D013936:Thymidine