The influence of LFA 1 on human T cells stimulated by solid-phase immobilized HLA class II-peptide complexes.
Purified HLA class II-peptide complexes immobilized to a solid support induce proliferation of human T-cell clones, indicating that human T-cell clones can proliferate in the absence of secondary signals from accessory cells. We hypothesized that T cells can provide co-stimulatory signals to T cells. LFA-1 molecules play an important role in homotypic interactions of T cells and murine monoclonal antibodies reactive with LFA 1 can inhibit T cell-T cell interactions. LFA-1 reactive monoclonal antibodies inhibited cytolysis of peptide-pulsed T cells by T cells and partially inhibited T-cell proliferation. To study the direct effect of the LFA 1 molecule on T-cell activation, we co-immobilized HLA-class II-peptide complexes with LFA-1 reactive MoAbs. Co-immobilization resulted in an enhanced proliferative response of the T-cell clones. This could indicate that the LFA 1 molecule on T cells is not a passive adhesion molecule, but is capable of transducing a signal that synergizes with the stimulatory signal via the T-cell receptor.
Van Schooten
W C
WC
Immunologic Pharmaceutical Corporation, Palo Alto CA 94304.
Quan
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002450:Cell Communication; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006681:HLA-D Antigens / Q000276:immunology*; D015804:HLA-DR4 Antigen / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D008024:Ligands; D008213:Lymphocyte Activation / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D051379:Mice; D010455:Peptides / Q000276:immunology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D015804:HLA-DR4 Antigen; C053495:HLA-Dw4 antigen; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1; D010455:Peptides