title: Fowlpox virus host range restriction: gene expression, DNA replication, and morphogenesis in nonpermissive mammalian cells.
abstract: Fowlpox virus (FPV), type species of the Avipoxvirus genus, causes a slow-spreading pox disease of chickens. Following infection of mammalian cells there is no evidence of productive replication of FPV although cytopathic effects are induced and FPV recombinants have been shown to express foreign genes from vaccinia virus early/late promoters. Here we report results of a study to investigate the expression of FPV genes, the replication of FPV genomic DNA, and any ultrastructural changes in mammalian cells infected by wild-type virus, undertaken as a first step in elucidating the nature of the block (or blocks) to productive replication of FPV in mammalian cells. Early and late gene expression as well as genomic DNA replication was observed in fibroblast-like cell lines of monkey and human origin. Furthermore, viral morphogenesis was observed in monkey cells, with the production mainly of immature particles though smaller numbers of apparently mature virus particles were observed.
authors.affiliation: A.E.R.C. Institute for Animal Health, Compton Laboratory, Nr. Newbury, Berkshire, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Somogyi
authors.forename: J
authors.initials: J
authors.lastname: Frazier
authors.forename: M A
authors.initials: MA
authors.lastname: Skinner
mesh_terms: D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002642:Chick Embryo; D003561:Cytarabine / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005587:Fowlpox virus / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure*; D015870:Gene Expression; D008715:Methionine / Q000378:metabolism; D009024:Morphogenesis; D013462:Sulfur Radioisotopes; D014616:Vaccinia virus / Q000235:genetics; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014779:Virus Replication
chemical_list: D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D003561:Cytarabine; D008715:Methionine