Cryopreservation of cultured dermal fibroblast impregnated collagen gels.
The survival of cultured dermal fibroblasts was evaluated following manufacture, freezing and disaggregation of fibroblast-impregnated collagen gels. The concentration which gave optimal cell survival was determined for three cryoprotectants (glycerol, dimethyl, sulphoxide (DMSO) and ethanediol) and their efficacy compared. DMSO led to the highest cell viability after freezing and thawing. The effect of rate of freezing was also compared and 0.5 degree C/min (within the range 20 degrees C to -70 degrees C) was found to result in a significant enhancement of cell viability in comparison with freezing at 1.0 degree C/min or rapid freezing.
Teasdale
B
B
Department of Anatomy, Queen Mary and Westfield College, University of London, UK.
Sieber
V K
VK
Riches
D J
DJ
Nanchahal
J
J
D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D005026:Ethylene Glycols; D005347:Fibroblasts / Q000166:cytology; D005615:Freezing; D005782:Gels; D005990:Glycerol; D006801:Humans; D012867:Skin / Q000166:cytology*
D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D005782:Gels; D003094:Collagen; D005990:Glycerol; D004121:Dimethyl Sulfoxide