Flow cytometry's contribution to the measurement of cell functions.
Flow cytometry has important advantages over conventional techniques. It is rapid, highly sensitive and allows multi-parametric analysis and cell sorting. Potential exists for the measurement of many cell functions by flow cytometry. The technique can be used to determine cell viability, intracellular calcium and pH, membrane potential, enzyme activities, membrane fluidity and endocytosis. Numerous examples are given on the applications of flow cytometry for cell functions measurements in the fundamental and biomedical fields.
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Institut de Biotechnologie, Limoges, France.
Huet
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Gallet
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Ratinaud
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D000818:Animals; D002118:Calcium / Q000032:analysis; D002462:Cell Membrane / Q000502:physiology; D002463:Cell Membrane Permeability; D002468:Cell Physiological Phenomena; D004705:Endocytosis; D004798:Enzymes / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000737:chemistry; D008560:Membrane Fluidity; D008564:Membrane Potentials; D010587:Phagocytosis
D004798:Enzymes; D002118:Calcium