Inhibition of the infectivity of influenza virus by tea polyphenols.
(-)Epigallocatechin gallate (EGCg) and theaflavin digallate (TF3) (1-10 microM) inhibited the infectivity of both influenza A virus and influenza B virus in Madin-Darby canine kidney (MDCK) cells in vitro. Study by electron microscope revealed that EGCg and TF3 (1 mM) agglutinated influenza viruses as well as did antibody, and that they prevented the viruses from adsorbing to MDCK cells. EGCg and TF3 more weakly inhibited adsorption of the viruses to MDCK cells. EGCg and TF3 (1-16 microM) also inhibited haemagglutination by influenza viruses. These findings suggest that tea polyphenols bind to the haemagglutinin of influenza virus, inhibit its adsorption to MDCK cells, and thus block its infectivity.
Nakayama
M
M
Department of Virology and Rickettsiology, National Institute of Health, Tokyo, Japan.
Suzuki
K
K
Toda
M
M
Okubo
S
S
Hara
Y
Y
Shimamura
T
T
D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D044946:Biflavonoids; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D004285:Dogs; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006387:Hemagglutination, Viral / Q000187:drug effects; D009980:Influenza A virus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity*; D009981:Influenza B virus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity*; D007668:Kidney / Q000187:drug effects / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000382:microbiology; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D013662:Tea; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D044946:Biflavonoids; D010936:Plant Extracts; D013662:Tea; C077197:theaflavin digallate; D005707:Gallic Acid; D002392:Catechin; C045651:epigallocatechin gallate