title: Interleukin-5 gene expression in nasal mucosa and changes in amount of interleukin-5 in nasal lavage fluid after antigen challenge.
abstract: Eosinophils and their products are known to cause hyperreactivity and swelling of the nasal mucosa in subjects with nasal allergy. Interleukin-5 (IL-5) not only induces differentiation and proliferation of immature eosinophils but also causes mature cells to accumulate and activate. This study shows that IL-5 is actually produced in the human nasal mucosa by antigen challenge, and it further investigates the changes in the amount of IL-5 in nasal lavage fluids after antigen challenge. Expression of mRNA for IL-5 in nasal mucosa was investigated using the reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. Among the 4 subjects with nasal allergy examined in this study, expression of mRNA for IL-5 was observed in 2 prior to antigen challenge; within 6 h after antigen challenge it was seen in 3 subjects. We also found that the amount of IL-5 in the nasal lavage fluids obtained consecutively after antigen challenge increased predominantly in the late phase, and that the number of eosinophils in the IL-5 positive group was significantly higher than that in the IL-5 negative group. These results strongly suggest that IL-5 contributes to the recruitment of eosinophils in the nasal mucosa of the subjects with nasal allergy.
authors.affiliation: Department of Otorhinolaryngology, Akita University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Terada
authors.forename: A
authors.initials: A
authors.lastname: Konno
authors.forename: S
authors.initials: S
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Shirotori
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Togawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000941:Antigens / Q000008:administration & dosage; D002648:Child; D004804:Eosinophils / Q000473:pathology / Q000502:physiology; D005260:Female; D015870:Gene Expression; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015848:Interleukin-5 / Q000032:analysis* / Q000235:genetics*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D009297:Nasal Mucosa / Q000737:chemistry / Q000378:metabolism*; D015508:Nasal Obstruction / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009299:Nasal Provocation Tests; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D000941:Antigens; D015848:Interleukin-5; D012333:RNA, Messenger