Influence of operation on glucose metabolism and lipolysis in human adipose tissue: a microdialysis study.
OBJECTIVE
To investigate the effect of an operation on metabolism in human adipose tissue.


METHODS
Open study.


METHODS
Department of Surgery and the Research Centre at Huddinge Hospital.


METHODS
13 non-obese patients who underwent elective cholecystectomy, and 5 control subjects.


METHODS
Microdialysis of the extracellular compartment of abdominal subcutaneous adipose tissue was done before, during and after a 45-minute intravenous infusion of 20 g glucose before, and one day after, the operation.


METHODS
Concentrations of glycerol, glucose, lactate and pyruvate in the tissue dialysate and in venous plasma.


RESULTS
After operation, the baseline plasma insulin concentration doubled (p < 0.001) and the fasting glucose concentration rose from 4.4 (0.1) to 5.3 (0.2) mmol/l (p < 0.001). In response to the glucose infusion, the maximum increase in plasma insulin trebled (p < 0.01) and the integrated concentrations (area under the curve) of plasma glucose, lactate and pyruvate were significantly more pronounced (p < 0.02-p < 0.001) after than before operation. The suppression of plasma glycerol and free fatty acid concentrations was similar before and after operation (50-60%). The reduction in the concentrations of glycerol in adipose tissue, and the increases in the concentrations of glucose, lactate, and pyruvate, after intravenous infusion of glucose were not influenced by the operation.


CONCLUSIONS
A moderately serious operation rapidly induces a deterioration in total body glucose homeostasis, partly as a result of peripheral insulin resistance. This does not affect the utilization of glucose by adipose tissue or the antilipolytic response to glucose infusion.
Felländer
G
G
Department of Surgery, Huddinge Hospital, Sweden.
Nordenström
J
J
Ungerstedt
U
U
Arner
P
P
Bolinder
J
J
D000273:Adipose Tissue / Q000378:metabolism*; D002763:Cholecystectomy; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000097:blood; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008066:Lipolysis; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D011773:Pyruvates / Q000097:blood; D019289:Pyruvic Acid; D013514:Surgical Procedures, Operative
D007328:Insulin; D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose