An analysis of the expression of cyclophilin C reveals tissue restriction and an intriguing pattern in the mouse kidney.
Cyclophilin C (cyp C) is a cyclosporin A (CsA) binding protein originally isolated from a mouse bone marrow stromal cell line. We have compared the expression patterns of the mammalian cyclophilins A, B, and C in mouse tissues using in situ hybridization. These studies reveal that cyp C is expressed in a restricted subset of tissues including mouse ovary, testis, bone marrow, and kidney. Within the kidney, cyp C is highly expressed in a narrow zone in the outer medulla. Using monoclonal antibodies reactive against cyp C, we find that the kidney cells expressing cyp C correspond to the S3 segment of the nephron. The S3 segment has been shown to sustain histopathological damage from high dosages of CsA, raising the possibility that cyp C may be involved in mediating this damage.
Friedman
J
J
Department of Pathology and Developmental Biology, Stanford University School of Medicine, CA 94305.
Weissman
I
I
Friedman
J
J
Alpert
S
S
D000591:Amino Acid Isomerases / Q000032:analysis* / Q000235:genetics; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D001483:Base Sequence; D001853:Bone Marrow / Q000737:chemistry; D001854:Bone Marrow Cells; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D000072622:Cyclophilin C; D021983:Cyclophilins; D004247:DNA / Q000032:analysis / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique; D017403:In Situ Hybridization; D007668:Kidney / Q000737:chemistry* / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009399:Nephrons / Q000737:chemistry / Q000473:pathology; D010053:Ovary / Q000737:chemistry / Q000166:cytology; D019696:Peptidylprolyl Isomerase; D016133:Polymerase Chain Reaction; D013737:Testis / Q000737:chemistry / Q000166:cytology
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; C081786:cyclophilin B; D004247:DNA; D000591:Amino Acid Isomerases; D021983:Cyclophilins; D000072622:Cyclophilin C; D019696:Peptidylprolyl Isomerase