Ethidium bromide intercalation and chromatin structure: a spectropolarimetric analysis.
Native chromatin has been characterized at different ionic strengths, according to different methods of preparation, by means of circular dichroism and ethidium bromide intercalation. For a more precise interpretation of these results, a series of analogous experiments on phagic DNA, as a function of its circularization, and on mononucleosomes have been performed. Results are discussed here in terms of high order chromatin structure, DNA supercoiling, and topological constraints.
Vergani
L
L
Institute of Biophysics, Medical School, University of Genoa, Italy.
Gavazzo
P
P
Mascetti
G
G
Nicolini
C
C
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002417:Cattle; D002843:Chromatin / Q000737:chemistry*; D002942:Circular Dichroism; D004278:DNA, Superhelical / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D004996:Ethidium / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011487:Protein Conformation
D002843:Chromatin; D004278:DNA, Superhelical; D004279:DNA, Viral; D004996:Ethidium