A comparative study of the kinetics and bioavailability of pure and soil-adsorbed naphthalene in dermally exposed male rats.
The aim of this study was to utilize pharmacokinetic techniques to assess the bioavailability of sandy or clay soil-adsorbed naphthalene vs chemical alone following dermal treatment of male rats. Animals were exposed to 43 micrograms total of 14C-naphthalene (pure or adsorbed to one of two soils) introduced into a shallow glass cap covering a 13-cm2 area on the skin of each rat. While both soils delayed the time to reach maximum plasma concentration of radioactivity and significantly increased the half-life of plasma absorption, only sandy soil significantly decreased the peak plasma concentration of radioactivity versus the pure compound. Within 12 h after dermal application, approximately 50% of the naphthalene dose was excreted in the urine of the pure and clay soil-adsorbed groups. However, when naphthalene was adsorbed to sandy soil, the percentages of the initial dose excreted in the urine collected between 0-12 h and 12-24 h were nearly equal (33-39%). Furthermore, sandy soil adsorption shifted the secondary excretion route from expired air to feces and significantly lowered the amount of radioactivity in expired air relative to naphthalene alone. In the presence of sandy soil, a significantly larger amount of radioactivity washed off of the skin application sites. In all groups the predominant urinary metabolites determined by high performance liquid chromatography were 2,7- and 1,2-dihydroxynaphthalenes.
Turkall
R M
RM
Pharmacology and Toxicology Department, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark 07103-2714.
Skowronski
G A
GA
Kadry
A M
AM
Abdel-Rahman
M S
MS
D000279:Administration, Cutaneous; D000818:Animals; D001682:Biological Availability; D008297:Male; D009281:Naphthalenes / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012307:Risk Factors; D012989:Soil Pollutants / Q000493:pharmacokinetics*
D009281:Naphthalenes; D012989:Soil Pollutants