Thin-layer chromatographic spray reagent for the screening of biological materials for the presence of carbaryl.
A spray reagent for the detection of the carbamate insecticide carbaryl was developed, consisting of 1% ammonium cerium(IV) nitrate in 20% v/v hydrochloric acid. This reagent reacts with the hydrolysis product of carbaryl, 1-naphthol, and forms a violet complex. Other carbamate insecticides do not give similar coloured spots. Moreover, organophosphorus, organochlorine and pyrethroid insecticides and constituents of visceral extracts (amino acids, peptides, proteins, etc.) do not interfere. The sensitivity of the reagent is about 0.1 microgram.
Patil
V B
VB
Department of Chemistry, Marathawada University, Aurangabad, India.
Shingare
M S
MS
D000818:Animals; D012721:Carbaryl / Q000032:analysis*; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D012721:Carbaryl