[The effect of hyperbaric oxygenation on H3-thymidine incorporation into human fibroblast cells with different ploidies].
The effect of different regimens of hyperbaric oxygenation (0.2 MPa and 0.4 MPa for 1 or 3 hour) has been investigated in the culture of diploid and triploid human fibroblasts, picked out from aborts. The quantity of marked thymidine in nuclei of cells depends on both the value of pressure and time of treatment. HBO causes stimulant effect under 3-hour treatment. Influence of different regimens of HBO on human fibroblasts reveals high resistance of triploid to oxidative stress in comparison with diploid fibroblasts.
Gus'kov
E P
EP
Shkurat
T P
TP
D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D006931:Hyperbaric Oxygenation; D011003:Ploidies; D013936:Thymidine / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine
Vliianie giperbaricheskoĭ oksigenatsii na vkliuchenie H3-timidina v kletki fibroblastov cheloveka raznoĭ ploidnosti.