Effect of phosphatidylserine on free radical susceptibility in human diploid fibroblasts.
We studied the effect of phosphatidylserine (PdtSER) on oxygen metabolite toxicity in skin fibroblast cell lines from apparently normal subjects. Fibroblast damage was produced by the generation of oxygen metabolites during the enzymatic oxidation of acetaldehyde by xanthine-oxidase (Xo). In order to quantify cell damage, we measured lactate dehydrogenase (LDH) activity in culture medium and cell viability in fibroblast cultures, with and without preincubation for 4 days with PdtSER 13 microM, after Xo incubation. We found a significant increase of LDH activity in culture medium of cells without preincubation with PdtSER. No significant increase of LDH activity was observed in the same cell lines after preincubation with PdtSER.
Latorraca
S
S
Department of Neurological and Psychiatric Sciences, University of Florence, Italy.
Piersanti
P
P
Tesco
G
G
Piacentini
S
S
Amaducci
L
L
Sorbi
S
S
D000079:Acetaldehyde / Q000378:metabolism; D002478:Cells, Cultured; D004171:Diploidy; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D005609:Free Radicals; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D010718:Phosphatidylserines / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism
D005609:Free Radicals; D010718:Phosphatidylserines; D007770:L-Lactate Dehydrogenase; D014969:Xanthine Oxidase; D000079:Acetaldehyde