Cloning and sequence determination of the gene coding for the pyruvate phosphate dikinase of Entamoeba histolytica.
We isolated three overlapping clones from a DNA genomic library of Entamoeba histolytica strain HM1:IMSS, whose translated nucleotide (nt) sequence shows similarities of 51, 48 and 47% with the amino acid (aa) sequences reported for the pyruvate phosphate dikinases from Bacteroides symbiosus, maize and Flaveria trinervia, respectively. The reading frame determined codes for a protein of 886 aa.
Saavedra-Lira
E
E
Departamento de Microbiologia, Instituto de Fisiología Celular, UNAM, México D.F., México.
Pérez-Montfort
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D016054:DNA, Protozoan / Q000032:analysis; D004748:Entamoeba histolytica / Q000201:enzymology / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics*; D008969:Molecular Sequence Data; D011772:Pyruvate, Orthophosphate Dikinase / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D016054:DNA, Protozoan; D011772:Pyruvate, Orthophosphate Dikinase