An investigation into the antigenic cross-reactivity of Ophiophagus hannah (king cobra) venom neurotoxin, phospholipase A2, hemorrhagin and L-amino acid oxidase using enzyme-linked immunosorbent assay.
The antigenic cross-reactivity of four Ophiophagus hannah (king cobra) venom components, the neurotoxin (OH-NTX), phospholipase A2 (OH-PLA2), hemorrhagin (OH-HMG) and L-amino acid oxidase (OH-LAAO) were examined by indirect and double sandwich ELISAs. The indirect ELISAs for OH-NTX, OH-PLA2 and OH-HMG were very specific when assayed against the various heterologous snake venoms and O. hannah venom components, at 25 ng/ml antigen level. At higher antigen concentrations (100-400 ng/ml), there were moderate to strong indirect ELISA cross-reactions between anti-O. hannah neurotoxin and venoms from various species of cobra as well as two short neurotoxins. However, anti-O. hannah hemorrhagin did not cross-react with any of the venoms tested, even at these high antigen concentrations, indicating that O. hannah hemorrhagin is antigenically very different from other venom hemorrhagins. Examination of the indirect ELISA cross-reactions between anti-O. hannah PLA2 and several elapid PLA2 enzymes suggests that the elapid PLA2 antigenic class has more than two subgroups. The antibodies to O. hannah L-amino acid oxidase, however, yielded indirect ELISA cross-reactions with many venoms as well as with OH-NTX, OH-PLA2 and OH-HMG, indicating that OH-LAAO shares common epitopes even with unrelated proteins. The double sandwich ELISAs for the four anti-O. hannah venom components, on the other hand, generally exhibited a higher degree of selectivity than the indirect ELISA procedure.
Tan
N H
NH
Department of Biochemistry, University of Malaya, Kuala Lumpur.
Lim
K K
KK
Jaafar
M I
MI
D000594:Amino Acid Oxidoreductases / Q000276:immunology*; D003039:Cobra Neurotoxin Proteins / Q000276:immunology*; D003429:Cross Reactions; D004546:Elapid Venoms / Q000737:chemistry / Q000276:immunology*; D010450:Endopeptidases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D050830:L-Amino Acid Oxidase; D010741:Phospholipases A / Q000276:immunology*; D054467:Phospholipases A2
D003039:Cobra Neurotoxin Proteins; D004546:Elapid Venoms; D000594:Amino Acid Oxidoreductases; D050830:L-Amino Acid Oxidase; D010741:Phospholipases A; D054467:Phospholipases A2; D010450:Endopeptidases; C041605:hemorrhagic proteinase IV