Evidence that human placenta is a site of sex hormone-binding globulin gene expression.
The presence of an androgen-binding component in placenta was investigated in vitro using a tissue culture system of human placental explants. Explants of trophoblastic tissue from normal term placentas were kept in culture under appropriate conditions for at least 48 h in a serum-free medium. The existence of an androgen-binding protein was explored by binding assays, immunohistochemistry studies and Northern blot analyses of placental mRNA. Steady-state polyacrylamide gel electrophoresis and Scatchard plot analyses revealed the presence of a high affinity specific binding component for 5 alpha-dihydrotestosterone in cultured placenta. Immunohistochemical studies performed on intact placenta and on Percoll-gradient purified trophoblastic cells demonstrated the presence of specific immunoreactivity in the cytoplasm of syncytial cells. Northern blot analyses of placental mRNA showed a single hybridizable 32P-labeled human sex hormone-binding globulin (SHBG) cDNA band of approx. 1.6 kb which was identical in size to that obtained with liver mRNA. The results strongly suggest the placenta as an origin of SHBG and point out this tissue as an additional site of SHBG synthesis during pregnancy.
Larrea
F
F
Department of Reproductive Biology, Instituto Nacional de la Nutrición Salvador Zubirán, Mexico City.
Díaz
L
L
Cariño
C
C
Larriva-Sahd
J
J
Carrillo
L
L
Orozco
H
H
Ulloa-Aguirre
A
A
D015153:Blotting, Western; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*
D012333:RNA, Messenger; D012738:Sex Hormone-Binding Globulin