title: Effect of fibrin sealant on the integrity of colonic anastomoses in rats with faecal peritonitis.
abstract: OBJECTIVE
  To assess the influence of fibrin adhesive on the healing of colonic anastomoses in rats with and without faecal peritonitis.
  
  
  METHODS
  Controlled study.
  
  
  METHODS
  Laboratory for experimental surgery, Erasmus University Rotterdam, The Netherlands.
  
  
  METHODS
  120 male Wag/Rij rats.
  
  
  METHODS
  All rats had a single layer end-to-end anastomosis fashioned with 7/0 polypropylene. Faecal peritonitis was then induced in half of the rats by placement of 200 mg powdered autoclaved rat faeces in the peritoneal cavity near the anastomosis. Rats were allocated to one of four groups (n = 30 in each): 1--control; 2--additional sealing with fibrin glue; 3--peritonitis alone; and 4--peritonitis with fibrin glue.
  
  
  METHODS
  Body weight, adhesion formation, anastomotic bursting pressure and collagen concentration around the anastomosis on days 2, 4, and 7 in 10 rats from each group.
  
  
  RESULTS
  11 rats died of peritonitis before the experiment was completed. Peritonitis caused increased formation of adhesions and abscesses, with or without fibrin sealant. Bursting pressure at the anastomosis was significantly reduced in peritonitis compared with controls on days 4 and 7, and this was not prevented by fibrin. Sealing of anastomoses resulted in lower bursting pressures on day 4 in control animals. Collagen concentration was significantly reduced in peritonitis with or without fibrin sealant on days 4 and 7, and after fibrin sealing of control anastomoses.
  
  
  CONCLUSIONS
  Faecal peritonitis reduced mechanical strength and collagen concentration of colonic anastomoses, and this was not prevented by additional sealing of the anastomosis with fibrin sealant.
authors.affiliation: Laboratory for Experimental Surgery, Erasmus University Rotterdam, The Netherlands.
authors.forename: A C
authors.initials: AC
authors.lastname: van der Ham
authors.forename: W J
authors.initials: WJ
authors.lastname: Kort
authors.forename: I M
authors.initials: IM
authors.lastname: Weijma
authors.forename: H F
authors.initials: HF
authors.lastname: van den Ingh
authors.forename: H
authors.initials: H
authors.lastname: Jeekel
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003094:Collagen / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000601:surgery*; D005243:Feces; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D008297:Male; D010538:Peritonitis / Q000150:complications* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013529:Surgical Wound Dehiscence / Q000209:etiology / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects
chemical_list: D015718:Fibrin Tissue Adhesive; D003094:Collagen