RasG protein accumulation occurs just prior to amoebae emergence during spore germination in Dictyostelium discoideum.
RasG protein levels in dormant and germinating spores of Dictyostelium discoideum strains JC1 and SG1 were estimated by Western blotting. RasG levels were very low in dormant spores and remained low during the lag period, regardless of whether spores were heat activated or treated with autoactivator during the early stages of spore germination. RasG levels increased late during spore swelling just prior to the emergence stage of germination. These data are consistent with a requirement for RasG during vegetative growth.
Khosla
M
M
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Spiegelman
G B
GB
Weeks
G
G
Sands
T W
TW
Virdy
K J
KJ
Cotter
D A
DA
D000818:Animals; D004023:Dictyostelium / Q000502:physiology*; D005656:Fungal Proteins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D013172:Spores, Fungal / Q000502:physiology; D018631:ras Proteins
D005656:Fungal Proteins; C489016:ras protein, Dictyostelium; D019204:GTP-Binding Proteins; D018631:ras Proteins