Determination of the content of water in bovine corneas by differential scanning calorimetry and thermogravimetric analysis.
The 'bound', 'bulk', and total water contents in bovine corneas were investigated using differential scanning calorimetry and thermogravimetric analysis. The amount of total water (expressed as a function of total wet mass) was observed to be 83.7 +/- 1.1% using lyophilization and 84.1 +/- 0.8% using thermogravimetric analysis. These values are not significantly different from one another, but some discrepancies are observed when these values are compared with the values reported in the literature, thus indicating a need for guidelines concerning sample handling and analysis of these tissues. The 'bound' water was observed to be 62.3 +/- 2.7 (% of total water) and the amount of 'bulk' water was calculated to be 20.8 +/- 2.5 (% of total water).
DeMali
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Department of Chemistry, College of Wooster, Ohio 44691.
Williams
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TR
D000818:Animals; D001834:Body Water; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002417:Cattle; D003315:Cornea / Q000737:chemistry*; D013818:Thermogravimetry / Q000379:methods; D014867:Water / Q000032:analysis
D014867:Water