Calcitonin receptor in human placental syncytiotrophoblast brush border and basal plasma membranes.
The physiology of calcium transport through the placenta has not been studied thoroughly. In particular, the effect of calcaemic hormones on this process has never been reported. In this paper we questioned if calcitonin, a hypocalcaemic hormone, is also implicated in the regulation of calcium transport by one of the placental syncytiotrophoblast bipolar membranes. In order to investigate the implication of calcitonin on calcium transport, we first studied whether this hormone binds to any of these bipolar membranes, i.e. purified syncytiotrophoblast brush border (facing the mother) and basal plasma membranes (facing the fetus). The initiation of binding of human [125I]calcitonin to the two types of membranes was rapid and reached a steady state after 10 min of incubation at 37 degrees C. The number of binding sites and the affinity of these receptors for the hormone were studied for each type of membrane, with concentrations of [125I]calcitonin varying from 0.01 to 1.8 nM. Scatchard analysis revealed a single affinity binding site for human calcitonin with Kds of 0.83 +/- 0.09 nM and 0.67 +/- 0.26 nM for brush border and basal plasma membranes respectively. The maximal number of receptors was significantly different (p < 0.001) in the two membranes: Bmax of 66.64 +/- 9.15 fmol/mg protein for brush border membranes and 19.66 +/- 2.80 fmol/mg protein for basal plasma membranes. Competitive displacement of [125I]calcitonin with other ligands showed the following potencies between human calcitonin > salmon calcitonin > calcitonin gene-related peptides and segments analogues but no competition with some human calcitonin gene-related peptides fragments.(ABSTRACT TRUNCATED AT 250 WORDS)
Lafond
J
J
Université du Québec à Montréal, Département des Sciences Biologiques, Canada.
Simoneau
L
L
Savard
R
R
Lajeunesse
D
D
D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D002116:Calcitonin / Q000378:metabolism / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D005260:Female; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007457:Iodine Radioisotopes; D008871:Microvilli / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D018003:Receptors, Calcitonin / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism* / Q000648:ultrastructure
D007295:Inositol Phosphates; D007457:Iodine Radioisotopes; D018003:Receptors, Calcitonin; C028815:salmon calcitonin; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide; D002118:Calcium