title: Analysis of rapid calcium signals in synaptosomes.
abstract: A combination of the stopped-flow technology with dual channel spectrofluorometry of Ca(2+)-indicators was utilized for the measurement of rapid Ca(2+)-signals in rat cerebral cortical synaptosomes evoked by K(+)-depolarization. There was no observable contribution of Ca(2+)-ions from intracellular stores to the rise in [Ca2+]i. The kinetics of the fast increase in intracellular Ca2+ concentration was analysed in relation to the depolarization strength. The maximal increase in [Ca2+]i and the time course of Ca(2+)-channel inactivation were determined for depolarizations obtained by different extracellular K(+)-concentrations ([K+]o). An apparent threshold was observed at about 18 mM [K+]o; a maximal Ca(2+)-signal amplitude was estimated at about 40 mM [K+]o. Pharmacological properties of the involved Ca(2+)-channels were determined using selective Ca(2+)-channel blockers (Dihydropyridines, omega-Conotoxin, omega-Agatoxins); the results suggest that a P-type voltage-dependent Ca(2+)-channel is the relevant channel type, generating the evoked Ca(2+)-signals in rat cerebral cortical synaptosomes.
authors.affiliation: University Stuttgart-Hohenheim, Institute of Zoophysiology, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Tareilus
authors.forename: J
authors.initials: J
authors.lastname: Schoch
authors.forename: M
authors.initials: M
authors.lastname: Adams
authors.forename: H
authors.initials: H
authors.lastname: Breer
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D002712:Chlorides / Q000494:pharmacology; D003035:Cobalt / Q000494:pharmacology; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors
chemical_list: D002712:Chlorides; D002104:Cadmium; D003035:Cobalt; D011189:Potassium Chloride; C018021:cobaltous chloride; D019256:Cadmium Chloride; D002118:Calcium