Sequence of a plant cDNA from Vicia faba encoding a novel Ran-related GTP-binding protein.
A clone obtained from a broad bean (Vicia faba) developing cotyledon cDNA library contained the complete coding sequence of a polypeptide with very high homology to the small GTP-binding proteins Ran from human cells and Spi1 from yeast. These proteins belong to the ras superfamily of proteins involved in different basic cellular processes. The Ran/Spi1 proteins interact with a protein bound to DNA (RCC1) and are thought to function in the regulation of the cell cycle. The amino acid sequence of the obtained plant Ran-homologue, designated Vfa-ran, is 74% and 76% identical to Ran and Spi1, respectively. The five functional, conserved domains of ras-related proteins are present in the Vfa-ran sequence. However, as in Ran/Spi1 the C-terminus of Vfa-ran is very acidic and lacks the Cys motif for isoprenylation. Northern blotting revealed a corresponding mRNA expression in broad bean roots, leaves, and cotyledons with the highest level in roots.
Saalbach
G
G
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Christov
V
V
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010946:Plants, Medicinal; D017386:Sequence Homology, Amino Acid; D020931:ran GTP-Binding Protein
D018076:DNA, Complementary; D010940:Plant Proteins; C087704:Vfa-ran protein, Vicia faba; D019204:GTP-Binding Proteins; D020931:ran GTP-Binding Protein