title: New insights suggesting a possible role of a heat shock protein 70-kD family-related protein in antigen processing/presentation phenomenon in humans.
abstract: A possible role of the peptide binding protein (PBP) 72/74 in antigen processing and presentation has been recently suggested in mice. In order to evaluate a possible analogous role of a PBP72/74-related protein in humans, immunoelectron microscope investigations, functional studies, and immunofluorescence analyses were performed on normal human peripheral antigen-presenting cells. We demonstrated that the determinant recognized by antiheat shock protein (HSP) 72/73 monoclonal antibody (MoAb) is constitutively expressed on the cell surface of monocytes as well as of B cells. Moreover, the capability of monocytes to present a recall antigen to T cells was significantly decreased when preincubated with an anti-HSP72/73 MoAb. These data add further strength to a potential role of a protein related to human PBP72/74 homologue in antigen processing and/or presentation. Finally, the capability of anti-HSP72/73 MoAb to impair the ability of fixed monocytes to present a synthetic peptide demonstrates that cell surface-localized PBP72/74-related protein could play a role in antigen presentation.
authors.affiliation: Department of Dermatology, University of Parma, Italy.
authors.forename: G C
authors.initials: GC
authors.lastname: Manara
authors.forename: P
authors.initials: P
authors.lastname: Sansoni
authors.forename: L
authors.initials: L
authors.lastname: Badiali-De Giorgi
authors.forename: G
authors.initials: G
authors.lastname: Gallinella
authors.forename: C
authors.initials: C
authors.lastname: Ferrari
authors.forename: V
authors.initials: V
authors.lastname: Brianti
authors.forename: F F
authors.initials: FF
authors.lastname: Fagnoni
authors.forename: C L
authors.initials: CL
authors.lastname: Ruegg
authors.forename: G
authors.initials: G
authors.lastname: De Panfilis
authors.forename: G
authors.initials: G
authors.lastname: Pasquinelli
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D017951:Antigen Presentation; D006684:HLA-DR Antigens / Q000502:physiology; D006360:Heat-Shock Proteins / Q000502:physiology*; D006801:Humans; D008213:Lymphocyte Activation; D008970:Molecular Weight; D009000:Monocytes / Q000276:immunology / Q000648:ultrastructure; D013745:Tetanus Toxoid / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D006360:Heat-Shock Proteins; D013745:Tetanus Toxoid