title: The actin-activated ATPase of co-polymer filaments of myosin and myosin-rod.
abstract: The actin activated ATPase of myosin at low ionic strength shows a complex dependence on actin concentration, in contrast with the simple hyperbolic actin activation kinetics of heavy meromyosin and subfragment-1. To investigate how the aggregation of myosin influences the actomyosin ATPase kinetics, we have studied the actin-activated ATPase of mixed filaments in which the myosin molecules are separated from each other by copolymerization with myosin rod. Electron microscopy of copolymer filaments, alone and bound to actin, indicates that the myosin heads are distributed randomly along the co-polymer filaments. The actin-activated ATPase of myosin decreases with increasing rod, approaching a plateau of about 30% of the control at a rod/myosin molar ratio of 4:1. The decrease in ATPase persists even at Vmax, the extrapolated limit at infinite actin, indicating that it is not due merely to the loss of cooperative actin binding. Furthermore, the actin dependence of the ATPase still shows a biphasic character like that of control myosin, even at rod/myosin ratio of 12:1, so this complexity is not probably due solely to the structural proximity of myosin molecules, but may involve a non-equivalence of myosin heads or myosin molecules in the filament environment.
authors.affiliation: Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa.
authors.forename: D
authors.initials: D
authors.lastname: Stepkowski
authors.forename: A A
authors.initials: AA
authors.lastname: Orlova
authors.forename: C
authors.initials: C
authors.lastname: Moos
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D007700:Kinetics; D008854:Microscopy, Electron; D009218:Myosins / Q000378:metabolism* / Q000648:ultrastructure; D011817:Rabbits
chemical_list: D000199:Actins; D000251:Adenosine Triphosphatases; D009218:Myosins