[The effect of antenatal nicotine exposure on the male germ cells in Wistar rats].
Chemically pure nicotine in dose 0.4, 1.5 and 5.0 mg/kg bw were percutaneously injected into Wistar rats during 1-20 (I) and 1-8 days (II) of pregnancy. The gametotoxic effect of nicotine in two-month males from offspring of the first generation (F1) was observed in disturbance of differentiation dynamics of spermatocytes at the stage of preleptotene and spermatids of the seventh developmental stage. The suggested quantitative analysis of disturbance in the behaviour of the meiotic chromosomes in metaphase I and II and anatelophase I and II has revealed that nicotine introduction in the period of isolation and migration of the primary sex cells into embryo gonads (2nd series) induces an increase in the level of pathological maturation divisions in the male offspring.
Khil'kevich
L V
LV
Kurilo
L F
LF
Sheveleva
G A
GA
D000818:Animals; D005260:Female; D005865:Gestational Age; D008297:Male; D008540:Meiosis / Q000187:drug effects; D009538:Nicotine / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar / Q000502:physiology*; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects*
D009538:Nicotine
Vliianie antenatal'nogo vozdeĭstviia nikotinom na muzhskie polovye kletki krys linii Vistar.