[The effect of hyaluronic acid on proliferation and differentiation of capillary endothelial cells].
The effects of hyaluronic acid (HA) on capillary endothelial cells were studied in cell culture. The capillary endothelial cells were obtained from the bovine adrenal cortex. The proliferation of endothelial cells was inhibited by the addition of HA to the culture medium. Significant inhibition was observed consistently at concentrations of 100 micrograms/ml or greater. Capillary-like networks were formed by the differentiated endothelial cells in co-culture with rabbit corneal keratocytes and in type I collagen gels. They were inhibited by the addition of HA at a concentration of 200 micrograms/ml. The cell proliferation and differentiation were not significantly affected by different HA molecular weights.
Watanabe
M
M
Department of Ophthalmology, National Defense Medical College, Tokorozawa, Japan.
Nakayasu
K
K
Okisaka
S
S
D000818:Animals; D002196:Capillaries; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003864:Depression, Chemical; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D006820:Hyaluronic Acid / Q000494:pharmacology*
D006820:Hyaluronic Acid