Alkali treatment for rapid preparation of plant material for reliable PCR analysis.
For plant genetics, it would be useful to monitor easily the segregation of different alleles using the polymerase chain reaction (PCR). Preparation of DNA templates from individual plants needs to be rapid and reliable. A one tube protocol is described that involves subjecting plant tissue pieces to alkali, neutralization and heat denaturation prior to PCR analysis, and that proved to be much faster and more reliable than published protocols.
Klimyuk
V I
VI
Sainsbury Laboratory, John Innes Centre, Norwich, UK.
Carroll
B J
BJ
Thomas
C M
CM
Jones
J D
JD
D000468:Alkalies; D001483:Base Sequence; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D010945:Plants, Edible / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D000468:Alkalies