title: Regulation of dihydrodipicolinate synthase and diaminopimelate decarboxylase activity in Bacillus stearothermophilus.
abstract: The feedback inhibition of the enzymes dihydrodipicolinate (DHDPS) and diaminopimelate decarboxylase (DAPD) in the wild strain Zu 183 of Bacillus stearothermophilus and in its S-(2-aminoethyl)-cysteine resistant L-lysine overproducing strain AEC 12 was studied. The optimum temperature and pH of both enzymes were also evaluated. No inhibition of DHDPS by L-lysine, L-threonine, L-methionine and L-isoleucine was observed either in the wild strain or in the AEC 12 mutant. DAPD was completely inhibited by L-lysine and only partially by L-threonine and L-methionine in Zu 183 and AEC 12 strains, but the concentration required was found to be much higher in the AEC 12 strain. The regulation mechanism of L-lysine biosynthesis in Bacillus stearothermophilus Zu 183 was also discussed.
authors.affiliation: Dipartimento di Biochimica, Ozzano Emilia (BO), Università di Bologna.
authors.forename: A
authors.initials: A
authors.lastname: Selli
authors.forename: F
authors.initials: F
authors.lastname: Crociani
authors.forename: D
authors.initials: D
authors.lastname: Di Gioia
authors.forename: F
authors.initials: F
authors.lastname: Fava
authors.forename: G
authors.initials: G
authors.lastname: Crisetig
authors.forename: D
authors.initials: D
authors.lastname: Matteuzzi
mesh_terms: D001426:Bacterial Proteins; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002851:Chromatography, High Pressure Liquid; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D006836:Hydro-Lyases / Q000037:antagonists & inhibitors*; D008239:Lysine / Q000494:pharmacology; D008715:Methionine / Q000494:pharmacology; D013912:Threonine / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D013912:Threonine; D008715:Methionine; D002262:Carboxy-Lyases; C079912:LysA protein, Bacteria; C020031:diaminopimelic acid decarboxylase; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase; D008239:Lysine