title: ELISA determination and preliminary pharmacokinetics of modified human rIL-1 beta in dogs.
abstract: A single dose pharmacokinetic study with modified human Interleukin-1 Beta (IL-1 beta or DuP 118) was performed by injecting 5 micrograms/kg of drug into the jugular vein of three beagle dogs. Serial 5-ml plasma samples were removed from the dogs over a 120-minute period. DuP 118 plasma levels were measured using a sandwich ELISA technique capable of measuring concentrations ranging from 0.25 to 2 ng/ml with accuracy and precision constraints of less than +/- 20% variability. DuP 118 stored at -20 degrees C in dog plasma was stable for at least 1 month. Pharmacokinetic parameters were determined for the three dogs by standard model independent or non-compartmental methods. DuP 118 was rapidly distributed in the dog. The volume of distribution was approximately two-fold higher than the total body water of a lean dog. The terminal half-life was short, less than 30 minutes. Within approximately an hour after dosing, DuP 118 plasma levels were reduced 20-fold and were below the quantifiable limit of the assay.
authors.affiliation: Drug Metabolism and Pharmacokinetics Section, Du Pont Merck Pharmaceutical Company, Newark, DE 19714.
authors.forename: J E
authors.initials: JE
authors.lastname: Gray
authors.forename: V
authors.initials: V
authors.lastname: Peterman
authors.forename: R
authors.initials: R
authors.lastname: Newton
authors.forename: S Y
authors.initials: SY
authors.lastname: King
authors.forename: H J
authors.initials: HJ
authors.lastname: Pieniaszek
mesh_terms: D000818:Animals; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000493:pharmacokinetics*; D011817:Rabbits; D011994:Recombinant Proteins / Q000493:pharmacokinetics
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins