title: Binding properties of protein A and protein G for human IgE.
abstract: We studied the binding of protein A and protein G to human monoclonal and polyclonal IgE. An ELISA was used to measure human IgE. Protein A-Sepharose did not bind to monoclonal IgE, but bound to 12-14% of serum polyclonal IgE and to -3% of the polyclonal IgE purified from the serum. No difference in binding to protein A was found between IgE from an allergic patient and that from a non-allergic subject. Protein G bound to neither polyclonal IgE nor monoclonal IgE. Thus, protein G is better than protein A with respect to separation of IgE from IgG.
authors.affiliation: Department of Paediatrics, University of Manitoba, Winnipeg, Canada.
authors.forename: Z
authors.initials: Z
authors.lastname: Peng
authors.forename: A B
authors.initials: AB
authors.lastname: Becker
authors.forename: F E
authors.initials: FE
authors.lastname: Simons
mesh_terms: D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000378:metabolism*
chemical_list: C029152:G-substrate; D009419:Nerve Tissue Proteins; D013205:Staphylococcal Protein A; D007073:Immunoglobulin E