Nuclear transformation of Volvox carteri.
Stable nuclear transformation of Volvox carteri was achieved using the cloned V. carteri nitA+ gene (which encodes nitrate reductase) to complement a nitA- mutation. Following bombardment of mutant cells with plasmid-coated gold particles, putative transformants able to utilize nitrate as a nitrogen source were recovered with an efficiency of approximately 2.5 x 10(5). DNA analysis indicated that the plasmid integrated into the genome, often in multiple copies, at sites other than the nitA locus. Cotransformants were recovered with a frequency of 40-80% when cells were cobombarded with a selected and an unselected marker. Thus, V. carteri becomes one of the simplest multicellular organisms that is accessible to detailed molecular studies of genes regulating cellular differentiation and morphogenesis.
Schiedlmeier
B
B
Lehrstuhl für Genetik, Universität Regensburg, Germany.
Schmitt
R
R
Müller
W
W
Kirk
M M
MM
Gruber
H
H
Mages
W
W
Kirk
D L
DL
D015139:Blotting, Southern; D002454:Cell Differentiation / Q000235:genetics; D000460:Chlorophyta / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D005819:Genetic Markers; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D010957:Plasmids; D014170:Transformation, Genetic
D005819:Genetic Markers; D009565:Nitrate Reductases; D050901:Nitrate Reductase