Antibody-antigen binding constants determined in solution-phase with the threshold membrane-capture system: binding constants for anti-fluorescein, anti-saxitoxin, and anti-ricin antibodies.
Affinities of various monoclonal and polyclonal antibodies for fluorescein-containing antigens, saxitoxin and ricin, were determined by using a light addressable potentiometric sensor-based system (Threshold). The dissociation constants, determined from Scatchard plots, ranged from 2 x 10(-7) to approximately 3 x 10(-12) M. Dissociation constants for fluorescein and saxitoxin were compared with values determined by independent means. This technique was found to be quick, simple, reproducible, and accurate.
Dill
K
K
Molecular Devices, Menlo Park, California 94025.
Lin
M
M
Poteras
C
C
Fraser
C
C
Hafeman
D G
DG
Owicki
J C
JC
Olson
J D
JD
D000906:Antibodies / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D019793:Fluorescein; D005452:Fluoresceins; D007118:Immunoassay / Q000379:methods; D007700:Kinetics; D011199:Potentiometry / Q000379:methods; D012276:Ricin / Q000276:immunology*; D012530:Saxitoxin / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D012530:Saxitoxin; D012276:Ricin; D019793:Fluorescein