title: The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein.
abstract: We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21,000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species.
authors.affiliation: Departamento de Genética e Evolução, Universidade Estadual de Campinas (UNICAMP), SP, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Prioli
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.forename: C S
authors.initials: CS
authors.lastname: Levings
mesh_terms: D002458:Cell Fractionation; D004272:DNA, Mitochondrial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D016366:Open Reading Frames; D010944:Plants / Q000235:genetics; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D003313:Zea mays / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins