title: Evidence for a site-specific endonuclease in yeast mitochondria which recognizes the sequence 5'GCCGGC.
abstract: We have discovered a mitochondrial, site-specific DNase in Saccharomyces cerevisiae with properties like that of a Type II restriction endonuclease. The enzyme, termed SceIII, cleaves the palindromic sequence 5'GCCGGC, to give 3' ends recessed by 4 bases. SceIII is the first restriction-like endonuclease to be described in yeast mitochondria.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
authors.forename: U R
authors.initials: UR
authors.lastname: Ezekiel
authors.forename: H P
authors.initials: HP
authors.lastname: Zassenhaus
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004720:Endonucleases / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004720:Endonucleases; D004262:DNA Restriction Enzymes