Selectable marker replacement in Saccharomyces cerevisiae.
Selectable markers integrated by the 'gamma' deletion method (Sikorski and Hieter, 1989) can be efficiently replaced in vivo with other markers by transformation with homologous plasmids. Transformation frequencies in experiments designed to replace original selectable markers with an alternate marker were high and molecular analysis confirmed that all transformants that exhibited the expected phenotypes (loss of the original prototrophy and gain of the alternate prototrophy) resulted from homologous recombination between plasmid sequences at the target locus. This technique involves no plasmid construction and greatly facilitates the generation of yeast cells containing multiple gene disruptions.
Vidal
M
M
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
Gaber
R F
RF
D005800:Genes, Fungal / Q000235:genetics*; D005819:Genetic Markers; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012641:Selection, Genetic; D017384:Sequence Deletion; D014170:Transformation, Genetic
D005819:Genetic Markers