Identification and localization of G(i) alpha 3 in the clonal adipocyte cell lines HGFu and Ob17.
The HGFu and Ob17 cell lines, derived from adipose tissue of lean (+/?) and ob/ob mice, respectively, express several G-protein peptides. Investigation of the expression and subcellular localization of the G(i) alpha 3 subunit showed that this peptide is associated with the Golgi apparatus. These findings indicate a role for this subunit in vesicular traffic and are in agreement with the view of the adipocyte as a secretory cell.
Cadrin
M
M
Department of Pathology, University of Ottawa, ON, Canada.
McFarlane-Anderson
N
N
Bégin-Heick
N
N
D017667:Adipocytes / Q000737:chemistry*; D000818:Animals; D002451:Cell Compartmentation; D002454:Cell Differentiation; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000302:isolation & purification*; D006056:Golgi Apparatus / Q000737:chemistry*; D051379:Mice; D008820:Mice, Obese
D019204:GTP-Binding Proteins