Circadian expression of the luciferin-binding protein correlates with the binding of a protein to the 3' untranslated region of its mRNA.
The circadian-expressed luciferin-binding protein from the dinoflagellate Gonyaulax polyedra is regulated at the translational level. We detected a protein, apparently a dimer, that binds specifically to the 3' untranslated region of its mRNA. Its binding site was localized within a 22-nt region in the 3' untranslated region containing seven UG repeats. The binding activity of this protein cycles on a daily basis, decreasing at the beginning of the night when synthesis of luciferin-binding protein starts and increasing at the end of the night when synthesis of luciferin-binding protein stops. This suggests that it functions as a clock-controlled repressor, preventing the translation of lbp mRNA during the day.
Mittag
M
M
Department of Cellular and Molecular Biology, Harvard University, Cambridge, MA 02138.
Lee
D H
DH
Hastings
J W
JW
D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002940:Circadian Rhythm; D004141:Dinoflagellida; D049454:Firefly Luciferin / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D014176:Protein Biosynthesis; D015800:Protozoan Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000502:physiology*
D002135:Calcium-Binding Proteins; D016376:Oligonucleotides, Antisense; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C065397:luciferin-binding protein, dinoflagellate; D049454:Firefly Luciferin