Residues 287-301 of human ribonuclease inhibitor do not affect ribonuclease activity and inhibitor binding; a reply.
Residues 287-301 of human placental ribonuclease inhibitor have been reported to inhibit pancreatic ribonuclease A in a similar way as the entire inhibitor (Crevel-Thieffry, I., Cotterill, S. and Schuller, E. (1992) Biochim. Biophys. Acta 1122, 107-112). Using three different assays, we were unable to observe inhibition by the synthetic peptide. Moreover, the peptide did not compete with the entire inhibitor for binding to RNAase A.
Fominaya
J
J
Friedrich Miescher-Institut, Basel, Switzerland.
Neumann
U
U
Hofsteenge
J
J
D000595:Amino Acid Sequence; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012260:Ribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism*
D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic