Use of thiocyanate ion as a mediator ligand in connecting acidic and alkaline forms of ferric myoglobin via paramagnetic 1H-NMR saturation transfer.
Paramagnetic 1H-NMR saturation transfer experiments have been used successfully to connect acidic and alkaline forms of equine ferric myoglobin through met-thiocyanate form as a mediator. The saturation transfer connectivities have provided the first assignments of the heme methyl proton resonances of equine met-hydroxyl myoglobin. Without mediator ligand, the acid-alkaline transition in this myoglobin is not accessible to the present technique due to the rapid exchange process between the two forms. Analysis of the intrinsic spin-lattice relaxation time and saturation transfer factor provided the life-time of met-thiocyanate and equine met-hydroxyl myoglobins under the experimental conditions used.
Yamamoto
Y
Y
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
D000143:Acids; D000468:Alkalies; D000818:Animals; D006736:Horses; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008786:Metmyoglobin / Q000737:chemistry*; D008956:Models, Chemical; D011522:Protons; D013861:Thiocyanates / Q000737:chemistry*
D000143:Acids; D000468:Alkalies; D008024:Ligands; D011522:Protons; D013861:Thiocyanates; D008786:Metmyoglobin