title: A non-specific lipid transfer protein from Arabidopsis is a cell wall protein.
abstract: Lipid transfer proteins (LTPs), mediate the transfer of phospholipids between membranes in vitro. However, the in vivo function of LTPs is not known. To determine the precise location of a non-specific LTP from Arabidopsis, a cDNA clone was used to produce an Arabidopsis LTP:protein A fusion. Antibodies raised against the fusion were used to localize the Arabidopsis LTP by immunoelectron microscopy. LTP was found to be located in the cell wall, mainly in epidermal cells. This location appears to be inconsistent with the proposed role of the protein in intracellular lipid transfer.
authors.affiliation: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824.
authors.forename: S
authors.initials: S
authors.lastname: Thoma
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneko
authors.forename: C
authors.initials: C
authors.lastname: Somerville
mesh_terms: D052179:Antigens, Plant; D017360:Arabidopsis / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002473:Cell Wall / Q000737:chemistry*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000235:genetics
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; C511920:lipid transfer proteins, plant