title: Sequences of the cobalamin biosynthetic genes cobK, cobL and cobM from Rhodococcus sp. NI86/21.
abstract: Sequence analysis of a 5753-bp genomic fragment of Rhodococcus sp. NI86/21 revealed the presence of three homologues of known cobalamin biosynthetic genes. One of these genes encodes a protein showing strong homology with the precorrin-6x reductase (CobK) of Pseudomonas denitrificans. In addition, the rhodococcal homologue of the corrin methyltransferases, CobM from P. denitrificans and CbiF from Salmonella typhimurium, was identified. The protein deduced from a third Rhodococcus gene aligns well with CobL of P. denitrificans and with the protein pair CbiE/CbiT of S. typhimurium, which are involved in corrin methylation and decarboxylation.
authors.affiliation: F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: De Mot
authors.forename: I
authors.initials: I
authors.lastname: Nagy
authors.forename: G
authors.initials: G
authors.lastname: Schoofs
authors.forename: J
authors.initials: J
authors.lastname: Vanderleyden
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics; D011549:Pseudomonas / Q000201:enzymology; D012240:Rhodococcus / Q000235:genetics*; D012486:Salmonella typhimurium / Q000201:enzymology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014805:Vitamin B 12 / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D010088:Oxidoreductases; C072472:precorrin 6x oxidoreductase; D008780:Methyltransferases; D014805:Vitamin B 12