title: Isolation and structure of harzianum A: a new trichothecene from Trichoderma harzianum.
abstract: A new trichothecene, harzianum A [1], was isolated from the soil-borne fungus Trichoderma harzianum. The structure of 1 was determined by extensive spectral analyses including the nmr techniques of PS-COSY, HMQC, HMBC, and NOESY. Harzianum A [1] contains a (Z,E,E)-2,4,6-octatriendioic acid esterified on the 4 beta hydroxyl group of trichodermol and is structurally related to the trichoverroids. Harzianum A [1] showed no cytotoxicity against baby hamster kidney cells, no activity against Gram-negative and Gram-positive bacteria, but modest antifungal activity at 100 micrograms/ml.
authors.affiliation: Monsanto Company, St. Louis, Missouri 63198.
authors.forename: D G
authors.initials: DG
authors.lastname: Corley
authors.forename: M
authors.initials: M
authors.lastname: Miller-Wideman
authors.forename: R C
authors.initials: RC
authors.lastname: Durley
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D006224:Cricetinae; D004354:Drug Screening Assays, Antitumor; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D014242:Trichoderma / Q000187:drug effects*; D014255:Trichothecenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D014255:Trichothecenes; C087546:harzianum A