Protein kinase C activation in Aplysia neurons by phorbol diacetate: comparison of effects following extracellular or intracellular application.
The effects of protein kinase C activation on electrophysiological phenomena of neurons in Aplysia californica ganglions were studied. The enzyme was activated by phorbol-12,13-diacetate applied either extracellularly by perfusion, or by intracellular pressure injection. In both forms of application, an increase in the potential upstroke speed and amplitude as well as a reduction of the depolarization evoked by extracellular acetylcholine application was found. A protein kinase C blocker, H-7, had opposite effects on the action potential. All observed actions of the phorbol ester were consistently faster in intracellular application than extracellular.
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Department of Neurophysiology, Max Planck Institue for Brain Research, Frankfurt/M., Germany.
Klee
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D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000109:Acetylcholine / Q000494:pharmacology; D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001048:Aplysia; D004789:Enzyme Activation / Q000187:drug effects; D007546:Isoquinolines / Q000494:pharmacology; D009474:Neurons / Q000201:enzymology*; D010703:Phorbol Esters / Q000008:administration & dosage / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*
D007546:Isoquinolines; D010703:Phorbol Esters; D010879:Piperazines; C008874:phorbol-12,13-diacetate; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D000109:Acetylcholine