title: Purification and properties of caldesmon-like protein from molluscan smooth muscle.
abstract: In this comparative study, the heat-stable protein content of scallop muscles was reinvestigated. The hCaD-like protein was prepared and its properties carefully examined. The heat-stable high-molecular-mass caldesmon-like (hCaD-like) protein is only present in the catch (smooth) muscle and it is completely absent in the striated muscle of scallop. The isolated scallop hCaD-like protein cosediments with F-actin, binds to myosin significantly and inhibits the ATPase activity of acto-myosin. A partial cDNA clone from a Mytilus anterior byssus retractor muscle (ABRM)-related protein showed strong homology with the hCaD gizzard sequence. This allowed identification of the heat-stable 100-110 kDa protein doublet band isolated in this study as a caldesmon-like molecule.
authors.affiliation: Department of Biochemistry, Eötvös Lorand University, Budapest, Hungary.
authors.forename: A M
authors.initials: AM
authors.lastname: Csizmadia
authors.forename: A
authors.initials: A
authors.lastname: Bonet-Kerrache
authors.forename: L
authors.initials: L
authors.lastname: Nyitray
authors.forename: D
authors.initials: D
authors.lastname: Mornet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D008974:Mollusca / Q000737:chemistry* / Q000235:genetics; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D009130:Muscle, Smooth / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D002148:Calmodulin-Binding Proteins; D009124:Muscle Proteins