Sperm motility loss induced by gossypol: relation with OH. scavengers, motile stimulators and malondialdehyde production.
Aerobic incubation of rat spermatozoa in a balanced salt solution containing 10 microM of gossypol produced a loss of sperm motility. This effect was not reverted by the presence of 25 mM Theophylline, a well-known stimulant of motility, but was significantly diminished by the addition of 10 mM Mannitol. As mannitol is a superoxide scavenger and we found a close correlation between the loss of sperm motility and the malondialdehyde production, we concluded that gossypol affect rat spermatozoa by producing a peroxidative breakdown of membrane phospholipids.
Fornés
M W
MW
Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
Barbieri
A M
AM
Burgos
M H
MH
D000332:Aerobiosis; D000818:Animals; D016166:Free Radical Scavengers; D006072:Gossypol / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D051381:Rats; D013081:Sperm Motility / Q000187:drug effects*; D013806:Theophylline / Q000494:pharmacology
D016166:Free Radical Scavengers; D008353:Mannitol; D008315:Malondialdehyde; D013806:Theophylline; D006072:Gossypol