Biologically active triterpene saponins from Bupleurum fruticosum.
Extracts of the root of B. fruticosum L. showed in a biological screening hemolytical activity, hepatoprotective and phagocytosis stimulating effects, and a specific inhibitory activity of leucine aminopeptidase. Further monitoring of the fraction with antihepatotoxic activity led to the isolation of an hepatoprotective saikosaponin identified as buddlejasaponin IV and the new malonylbuddlejasaponin IV, determined as saikogenin F-3-O-[6-O-malonyl-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl (1-->3)]-beta-D-fucopyranoside. The structures were elucidated on the basis of the chemical and spectroscopic data.
Guinea
M C
MC
Departmento de Farmacia y Tecnología Farmacéutica, Universidad de Alcalá de Henares, Madrid, Spain.
Parellada
J
J
Lacaille-Dubois
M A
MA
Wagner
H
H
D000818:Animals; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007931:Leucyl Aminopeptidase / Q000037:antagonists & inhibitors; D008099:Liver / Q000187:drug effects; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D006460:Hemolysin Proteins; D012503:Saponins; D014315:Triterpenes; D007931:Leucyl Aminopeptidase