Immunohistochemical microquantitation method for type I collagen in kidney histological section of the rats.
An immunohistochemical micromethod for quantitation of type I collagen in frozen histological section of rat kidney was developed. A principle of this method is enzyme-linked immunosorbent assay (ELISA). The intensity of immunoreactivity of the section to an anti-type I collagen antibody was quantitated, and then the total protein content in the same section was measured colorimetrically. Type I collagen content was expressed as mg of type I collagen per g of total protein. In this micromethod, the minimum area and optimum thickness of the section were 5 mm2 and 10 microns, respectively. Type I collagen content in the rat kidney section was 3.02 +/- 0.12 mg/g. This method should be useful for diagnosis of renal failure.
Manabe
N
N
Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
Furuya
Y
Y
Nagano
N
N
Miyamoto
H
H
D000818:Animals; D003094:Collagen / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000166:cytology*; D007672:Kidney Cortex / Q000166:cytology; D008297:Male; D008832:Microchemistry / Q000379:methods; D051381:Rats; D017208:Rats, Wistar
D003094:Collagen