title: Developmental regulation of growth hormone binding in the gilthead sea bream, Sparus aurata.
abstract: The occurrence of growth hormone (GH) binding was investigated in the gilthead sea bream larva, Sparus aurata, using recombinant trout GHII (rtGH) as a tracer. GH binding was detected in all the specimens examined (5, 17, 27 and 40 days after hatching). At 40 days after hatching, we observed a concentration of GH binding in the head region. Specific binding was dependent on protein membrane concentration and Scatchard plots showed a single class of high affinity and low capacity binding sites. Specific binding was inhibited by rtGH and ovine GH in a dose dependent manner. Chinook salmon prolactin and chinook salmon gonadotropin did not compete for 125I-rtGH binding sites. To our knowledge, this is the first report that indicates the presence of specific and saturable GH binding sites during early growth and development of a fish species.
authors.affiliation: Instituto de Acuicultura de Torre de la Sal, CSIC, Castellón, Spain.
authors.forename: H
authors.initials: H
authors.lastname: Martí-Palanca
authors.forename: J
authors.initials: J
authors.lastname: Pérez-Sánchez
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D010473:Perciformes / Q000254:growth & development* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014337:Trout
chemical_list: D011994:Recombinant Proteins; D013006:Growth Hormone