Selective inactivation of viruses in the presence of human platelets: UV sensitization with psoralen derivatives.
Inactivation of viruses in blood products requires that the method employed display selectivity in its action for viral elements while not affecting the biological entity of interest. Several methods have been developed for the treatment of human plasma or products derived from human plasma. An effective technique for the treatment of the cellular components of blood has been lacking, in part due to the inability to develop agents capable of selectively targeting viral agents in the milieu of cellular material. In this paper, we examine the behavior of a group of viral sensitizers designed to be added to cellular samples and be activated upon exposure to UVA light. Upon activation, these agents are capable of disrupting nucleic acids of the virus in a manner that renders them inactive for proliferation. The selectivity observed in this inactivation is determined by the chemical structure of the sensitizer, which can be varied to increase viral killing capacity while diminishing collateral damage to cellular and protein constituents.
Goodrich
R P
RP
Cryopharm Corporation, Pasadena, CA 91107.
Yerram
N R
NR
Tay-Goodrich
B H
BH
Forster
P
P
Platz
M S
MS
Kasturi
C
C
Park
S C
SC
Aebischer
N J
NJ
Rai
S
S
Kulaga
L
L
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000998:Antiviral Agents; D001435:Bacteriophages / Q000187:drug effects* / Q000528:radiation effects*; D001792:Blood Platelets / Q000382:microbiology*; D003094:Collagen / Q000494:pharmacology; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D011838:Radiation-Sensitizing Agents; D014466:Ultraviolet Rays
D000998:Antiviral Agents; D011564:Furocoumarins; D011838:Radiation-Sensitizing Agents; D000244:Adenosine Diphosphate; D003094:Collagen