title: Screening method for the determination of volatiles in biomedical samples by means of an off-line closed-loop trapping system and high-resolution gas chromatography-ion trap detection.
abstract: A method is described for the analysis of volatile organic compounds in biological matrices (faeces and urine). The technique is based on off-line preconcentration by means of a closed-loop trapping system followed by high-resolution gas chromatography-ion trap detection (HRGC-ITD) for separation and identification of the compounds. The technique has been validated for pattern recognition in faecal and urine samples from healthy volunteers. It is considered a very promising tool in metabolic research.
authors.affiliation: Department of Gastrointestinal Research, University Hospital Gasthuisberg, K.U. Leuven, Belgium.
authors.forename: Y
authors.initials: Y
authors.lastname: Ghoos
authors.forename: D
authors.initials: D
authors.lastname: Claus
authors.forename: B
authors.initials: B
authors.lastname: Geypens
authors.forename: M
authors.initials: M
authors.lastname: Hiele
authors.forename: B
authors.initials: B
authors.lastname: Maes
authors.forename: P
authors.initials: P
authors.lastname: Rutgeerts
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D005243:Feces / Q000737:chemistry*; D005285:Fermentation; D006801:Humans; D006838:Hydrocarbons / Q000032:analysis*; D007202:Indicators and Reagents; D008055:Lipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011786:Quality Control; D014556:Urine / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D006838:Hydrocarbons; D007202:Indicators and Reagents; D008055:Lipids; D011506:Proteins