Three-way differentiation of Chinese hamster ovary chromosomes by immunoperoxidase technique using a monoclonal anti-bromodeoxyuridine antibody.
A new permanent staining procedure for sister chromatid differentiation (SCD) in cultured Chinese hamster ovary cells has been established by combining the three-way differentiation in third mitosis (M3) chromosomes and the immunoperoxidase reaction developed with 3,3'-diaminobenzidine using a monoclonal anti-bromodeoxyuridine (BrdU) antibody. This procedure allows SCD at very low BrdU concentrations, and the evaluation of the sister chromatid exchange frequencies on a per cell-cycle basis.
Piñero
J
J
Department of Cell Biology, University of Seville, Spain.
Ortiz
T
T
Cortés
F
F
D015100:3,3'-Diaminobenzidine; D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D016466:CHO Cells; D002842:Chromatids / Q000648:ultrastructure; D006224:Cricetinae; D004277:DNA, Single-Stranded / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D008938:Mitosis; D012854:Sister Chromatid Exchange
D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D015100:3,3'-Diaminobenzidine; D001973:Bromodeoxyuridine