Alternative pathways of sulfite oxidation in human polymorphonuclear leukocytes.
Sodium sulfite is metabolized by human polymorphonuclear leukocytes by two alternative pathways, one enzymatic route dependent on sulfite oxidase and one non-enzymatic which involves intermediate formation of sulfur trioxide anion radicals. Initiation of the oxidative burst by phorbol myristate acetate significantly stimulates sulfate formation through the second pathway. The activity of sulfite oxidase in polymorphonuclear leukocytes varies greatly among individuals, a variation consistent with the suggested polymorphic distribution of sulfite oxidase in the human population.
Constantin
D
D
Division of Toxicology, Karolinska Institutet, Stockholm, Sweden.
Mehrotra
K
K
Jernström
B
B
Tomasi
A
A
Moldéus
P
P
D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000097:blood; D010101:Oxygen Consumption; D016897:Respiratory Burst / Q000187:drug effects; D013447:Sulfites / Q000097:blood*; D013481:Superoxides / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D013447:Sulfites; D013481:Superoxides; D050862:Oxidoreductases Acting on Sulfur Group Donors; D013755:Tetradecanoylphorbol Acetate; C025026:sodium sulfite