title: Allergen-induced expression of cell surface markers on lymphocytes of Chi t I-sensitized patients.
abstract: Lymphocyte cultures of persons sensitized to the hemoglobin allergen Chi t I show a highly significant response to the allergen measured in the lymphocyte stimulation assay by (3H)-thymidine uptake. In this study, we investigated by flow cytometry the expression of different cell surface markers on lymphocytes after in vitro stimulation for 7 d with or without the allergen Chi t I. We determined the expression of the low-affinity receptor for IgE (CD23) on lymphocytes of Chi t I-sensitized patients and Chi t I-exposed as well as nonexposed controls. CD23 expression was significantly higher in patients than in nonexposed controls. Exposed but healthy subjects showed intermediate values. We also determined the expression of activation markers CD25 (IL-2 receptor) and HLA-DR on the lymphocytes of patients and nonexposed controls. HLA-DR expression on non-T cells (CD3-) was significantly higher in patients than in controls. HLA-DR on T cells (CD3+), and CD25 as well as CD23 expression, could be significantly enhanced after antigen-specific stimulation in patients but not in controls, whereas alpha/beta-T-cell-receptor expression was significantly reduced in patients. Differences between patients and controls were not observed in response to tetanus toxoid (TT) and phytohemagglutinin (PHA). Our results demonstrate antigen-specific influences on the expression of cell surface molecules. These findings may be valuable diagnostic information.
authors.affiliation: Berufsgenossenschaftliches Forschungsinstitut für Arbeitsmedizin (BGFA), Ruhr-Universität Bochum, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Liebers
authors.forename: M
authors.initials: M
authors.lastname: Raulf
authors.forename: X
authors.initials: X
authors.lastname: Baur
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016516:CD4-CD8 Ratio; D002478:Cells, Cultured; D002683:Chironomidae / Q000276:immunology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006454:Hemoglobins / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013745:Tetanus Toxoid / Q000276:immunology; D013997:Time Factors
chemical_list: D000485:Allergens; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D006454:Hemoglobins; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D013745:Tetanus Toxoid; D007073:Immunoglobulin E