title: T cell subset responses to allogeneic endothelium. Proliferation of CD8+ but not CD4+ lymphocytes.
abstract: Endothelial cells were isolated from the hearts of neonatal mice and cocultured with syngeneic and allogeneic lymphocytes. T cells isolated by passage through nylon wool proliferated when cultured with allogeneic endothelium but not when cultured with syngeneic endothelium. This response was almost entirely confined to the CD8+ lymphocyte subset as purified CD4+ lymphocytes displayed a minimal response. Pretreatment of endothelial cells with recombinant murine gamma interferon induced expression of Ia but did not enable endothelial cells to activate CD4+ lymphocytes. Activation of CD8+ and T lymphocytes could be blocked with monoclonal anti class I antibody but was unaffected by anti-class II antibody. The failure to activate CD4+ lymphocytes was not due to suppression and did not lead to an anergic state. Instead, coculture of CD4+ cells with allogeneic endothelial cells induced a partial activation consisting of IL-2 receptor expression and accelerated secondary response kinetics.
authors.affiliation: Department of Surgery, University of Vermont, Burlington 05405.
authors.forename: P A
authors.initials: PA
authors.lastname: Lodge
authors.forename: C E
authors.initials: CE
authors.lastname: Haisch
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002455:Cell Division; D002460:Cell Line / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology; D016027:Heart Transplantation / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology; D013154:Spleen / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D014184:Transplantation, Homologous
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D000949:Histocompatibility Antigens Class II