An improved method to calculate adipose tissue interstitial substrate recovery for microdialysis studies.
We simultaneously compared the conventional, time-consuming point of no net flux method for calculation of interstitial substrate recovery necessary for in vivo microdialysis studies with a simple isotopic method using rat epididymal fat pads. The recovery (%) calculated with the conventional method and the isotopic method for glucose (7.4 +/- 1.1 vs. 6.6 +/- 0.6), glycerol (23 +/- 4 vs. 26 +/- 5) and lactate (40 +/- 8 vs. 38 +/- 5), respectively, were not significantly different. Moreover, the overall correlation coefficient (N = 25) between the methods was 0.87, p < 0.001. We therefore conclude that the methods yield comparable results, and the more convenient isotopic method should become the method of choice for determining adipose tissue interstitial recovery for glucose, lactate and glycerol.
Jansson
P A
PA
Whittier Institute for Diabetes and Endocrinology, La Jolla, CA 92037.
Veneman
T
T
Nurjhan
N
N
Gerich
J
J
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D005110:Extracellular Space / Q000378:metabolism*; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D005990:Glycerol / Q000032:analysis / Q000378:metabolism; D007773:Lactates / Q000032:analysis / Q000378:metabolism; D008297:Male; D017551:Microdialysis / Q000379:methods*; D011865:Radioisotope Dilution Technique; D051381:Rats; D017207:Rats, Sprague-Dawley; D014316:Tritium
D002250:Carbon Radioisotopes; D007773:Lactates; D014316:Tritium; D005947:Glucose; D005990:Glycerol