title: The Escherichia coli efg gene and the Rhodobacter capsulatus adgA gene code for NH3-dependent NAD synthetase.
abstract: The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase. Crude extracts from a strain which overproduces the efg gene product contained up to 400 times more activity than crude extracts from the control strain, and the purified Efg protein possessed-NH3-dependent NAD synthetase activity. Glutamine-dependent NAD synthetase activity was found in crude extracts of E. coli but not in the purified enzyme, suggesting that it may be catalyzed by an additional subunit. An R. capsulatus strain carrying an adgA mutation was found to be deficient in NAD synthetase activity, and activity was restored by complementation with the E. coli gene. In accordance with the nomenclature proposed for Salmonella typhimurium (K. T. Hughes, B. M. Olivera, and J. R. Roth, J. Bacteriol. 170:2113-2120, 1988), the efg and adgA genes should now be designated nadE.
authors.affiliation: Laboratoire de Biochimie Microbinne (Centre National de la Recherche Scientifique URA 1130), Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires de Grenoble, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Willison
authors.forename: G
authors.initials: G
authors.lastname: Tissot
mesh_terms: D019732:Amide Synthases; D000641:Ammonia / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D008025:Ligases / Q000032:analysis / Q000235:genetics*; D009243:NAD / Q000031:analogs & derivatives / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000235:genetics*
chemical_list: D009243:NAD; C018348:nicotinic acid adenine dinucleotide; D000641:Ammonia; D008025:Ligases; D019732:Amide Synthases; C038479:NAD+ synthase