Human angiotensinogen is highly expressed in astrocytes in human cortical grafts.
Human fetal parietal cortical tissue was transplanted to cortical cavities in immunosuppressed rats. Protoplasmic astrocytes in the human cortical grafts highly expressed human angiotensinogen mRNA as identified with 35S-labeled and digoxigenin-labeled riboprobes combined with immunohistochemistry for glial fibrillary acidic protein. Antibodies to human specific neurofilament protein 70 KD were used to characterize neurons in the graft and fiber outgrowth into the host brain. Immunohistochemistry revealed human angiotensinogen-like immunoreactivity in many small protoplasmic astrocytes and very few large neurons. These results demonstrate that human angiotensinogen mRNA and protein is synthesized in immature human glia. We assume that angiotensinogen is transformed into angiotensin peptides, which may participate in the regulation of growth processes. The results suggest that human angiotensinogen may play a role during human embryogenesis.
Humpel
C
C
Department Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
Lippoldt
A
A
Strömberg
I
I
Bygdeman
M
M
Wagner
J
J
Hilgenfeldt
U
U
Ganten
D
D
Fuxe
K
K
Olson
L
L
D000808:Angiotensinogen / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005260:Female; D016332:Fetal Tissue Transplantation; D006801:Humans; D007150:Immunohistochemistry; D010296:Parietal Lobe / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D000808:Angiotensinogen