Vacuolar ATPase inactivation blocks recycling to the trans-Golgi network from the plasma membrane.
TGN38/41 is an integral membrane protein which recycles between the trans-Golgi network (TGN) and the cell surface but is predominantly located in the TGN of rat (NRK) cells at steady state. As part of our studies on the mechanism and route of recycling between the TGN and the cell surface we have used chloroquine or Bafilomycin A1 to modulate the lumenal pH of endocytic organelles. The data we present demonstrate that inactivation of the proton pump which maintains the acidic environment within the lumen of endocytic organelles leads to an accumulation of TGN38/41 in early endosomes. These data confirm the observation that TGN38/41 recycles between the plasma membrane and the TGN and identifies a specific block in that recycling pathway.
Reaves
B
B
Department of Biochemistry, School of Medical Sciences, University of Bristol, UK.
Banting
G
G
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins; D006056:Golgi Apparatus / Q000378:metabolism; D018942:Macrolides; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins; D017494:Proton Pumps / Q000187:drug effects*; D051381:Rats; D014617:Vacuoles / Q000201:enzymology
D000900:Anti-Bacterial Agents; D006023:Glycoproteins; D018942:Macrolides; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D017494:Proton Pumps; C513045:Tgoln2 protein, rat; D002738:Chloroquine; C040929:bafilomycin A1; D000251:Adenosine Triphosphatases