title: Effects of acidosis and alkalosis on mechanical properties of hypertrophied rat heart fiber bundles.
abstract: Effects of alkalosis (pH 7.4) or acidosis (pH 6.8) on the intrinsic mechanical properties of control and pressure-overloaded rat hearts were studied in Triton X-100-treated left ventricular fiber bundles. In control bundles, Ca sensitivity [pCa required for one-half maximal response (pCa50)] was 5.520 +/- 0.012 at pH 7.1. Alkalosis increased it by 0.357 +/- 0.018 pCa unit, whereas acidosis decreased it by 0.365 +/- 0.014 pCa unit with no change in Hill coefficient. Maximal tension was decreased by acidic pH and increased by alkaline pH. Stiffness was measured by the response to quick length changes. Acidosis decreased maximal stiffness but increased the stiffness-to-force ratio, whereas alkalosis increased maximal stiffness but had no effect on stiffness-to-force ratio, suggesting that acidosis decreased the force generated per cross bridge. Alkalosis increased the time constant of tension recovery following a quick stretch from 10.6 +/- 0.66 to 17.45 +/- 1.83 ms, suggesting a decreased cross-bridge cycling rate. Pressure overload induced by thoracic aortic stenosis for 4-6 wk led to a 200% cardiac hypertrophy associated with a shift from fast to slow ventricular myosin. pCa50 of hypertrophied bundles was not different from control (5.541 +/- 0.012). Ca sensitivity was increased by 0.383 +/- 0.008 in alkaline medium and decreased by 0.325 +/- 0.009 in acidic medium. Stiffness-to-force ratio was decreased in acidic pH, and the time constant of tension recovery was increased from 31.0 +/- 0.4 to 34.9 +/- 0.25 ms by alkalosis. In hypertrophied bundles, maximal tension was decreased by acidic pH but not changed by alkalosis. These results show that in the small pH range of our study 1) pH changes have symmetrical effects on Ca sensitivity in both control and hypertrophied bundles, 2) a decrease or an increase in H+ concentration does not have symmetrical effects on the mechanics of the cross bridges, and 3) changes in the phenotype of contractile proteins induced by aortic stenosis do not influence Ca sensitivity, only moderately influence the response to pH changes, and mainly affect the cross-bridge cycling rate.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Université de Paris Sud, Châtenay-Malabry, France.
authors.forename: E
authors.initials: E
authors.lastname: Mayoux
authors.forename: N
authors.initials: N
authors.lastname: Coutry
authors.forename: P
authors.initials: P
authors.lastname: Lechêne
authors.forename: F
authors.initials: F
authors.lastname: Marotte
authors.forename: C
authors.initials: C
authors.lastname: Hoffmann
authors.forename: R
authors.initials: R
authors.lastname: Ventura-Clapier
mesh_terms: D000138:Acidosis / Q000503:physiopathology; D000471:Alkalosis / Q000503:physiopathology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D006332:Cardiomegaly / Q000503:physiopathology*; D006321:Heart / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009200:Myocardial Contraction; D009218:Myosins / Q000378:metabolism; D009929:Organ Size; D010210:Papillary Muscles / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values
chemical_list: D009218:Myosins; D002118:Calcium