Cloning of novel Wilms tumor gene (WT1) cDNAs; evidence for antisense transcription of WT1.
WT1 is a tumor suppressor gene that has been implicated in Wilms tumor, and is expressed in cells of mesodermal origin. The Wit-1 gene is located approximately 2 kb from the WT1 gene, and is expressed coordinately with WT1. WT1 and Wit-1 are bi-directionally transcribed from the same promoter region. We have screened a human fetal kidney cDNA library to identify novel WT1 cDNA clones. Here we report the cloning of cDNA clones which span part of intron 1 of WT1, exon 1, upstream sequences between WT1 and Wit-1 and part of the Wit-1 gene. Northern blot and RNAase protection analysis using subcloned fragments of the cDNAs corresponding to regions from within intron 1 of WT1 suggest that a 7-10 Kb RNA is expressed in human fetal kidney, which overlaps with WT1 and is transcribed in the same direction as Wit-1.
Eccles
M R
MR
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Grubb
G
G
Ogawa
O
O
Szeto
J
J
Reeve
A E
AE
D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D016162:Genes, Wilms Tumor; D006801:Humans; D007438:Introns; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense