Localization of MPM-2 recognized phosphoproteins and tubulin during cell cycle progression in synchronized Vicia faba root meristem cells.
MPM-2 antibody reacts with a subset of mitotic phosphoproteins. We followed localization of MPM-2 immunoreactive material and localization of microtubules during cell cycle progression in a highly synchronous population of Vicia faba root meristem cells and isolated nuclei. The MPM-2 antibody labelling showed significant cell cycle dependence. MPM-2 nuclear reactivity was weak and homogeneous in G1 and S phase of the cell cycle and became stronger and heterogeneous during G2, resembling staining of the nuclear matrix, with maximum staining at the G2/M interface. Similarly the staining intensity of nucleoli increased from late G1 phase to nucleoli dispersion in early prophase. During mitosis MPM-2 immunoreactivity was associated with spindle configurations and the brightest signal was localized in kinetochores from prophase to metaphase.
Binarova
P
P
Czech Academy of Sciences, Institute of Experimental Botany, Olomouc.
Cihalikova
J
J
Dolezel
J
J
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002453:Cell Cycle; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D002478:Cells, Cultured; D007887:Fabaceae / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D010750:Phosphoproteins / Q000032:analysis* / Q000276:immunology; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology; D010946:Plants, Medicinal; D008941:Spindle Apparatus / Q000737:chemistry / Q000648:ultrastructure; D013234:Stem Cells / Q000737:chemistry / Q000648:ultrastructure; D014404:Tubulin / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D010750:Phosphoproteins; D010940:Plant Proteins; D014404:Tubulin