B lymphocyte immunoglobulin receptor desensitization is downstream of tyrosine kinase activation.
Resting mature B cells have two classes of immunoglobulin receptors on their surface, IgD and IgM. Activation of a cell by crosslinking one of these receptors leads to homologous and heterologous receptor anergy as judged by the inability to induce a second calcium signal 2 hr after the initial activation step. The mechanism for this energy is not known. In this report we show that this receptor anergy is downstream of tyrosine kinase activation in that cells pretreated with anti-IgM, when stimulated with anti-IgD, showed tyrosine phosphorylation comparable to that of naive cells, but had no calcium response.
Brunswick
M
M
Laboratory of Molecular and Developmental Immunology, Food and Drug Administration, Bethesda, Maryland 20892.
June
C H
CH
Mond
J J
JJ
D000818:Animals; D004789:Enzyme Activation; D051379:Mice; D008811:Mice, Inbred DBA; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D015398:Signal Transduction; D013997:Time Factors
D011947:Receptors, Antigen, B-Cell; D011505:Protein-Tyrosine Kinases