Mechanisms involved in the cardiovascular-renal actions of nitric oxide inhibition.
The roles of the sympathetic nervous system, angiotensin II, and arginine vasopressin in the cardiovascular-renal responses to nitric oxide synthesis inhibition were examined in eight conscious dogs equipped with arterial and venous catheters and a nonoccluding bladder catheter. Nitric oxide inhibition was achieved by intravenous infusion of NG-nitro-L-arginine methyl ester (L-NAME) at 37.1 nmol/kg per minute for 140 minutes in the control group. The same dogs, after a 1-week recovery, were pretreated for 2 days with either prazosin for alpha 1 blockade, prazosin plus propranolol for alpha 1 plus beta blockade, L-158,809 for angiotensin receptor blockade, or d(CH2)Tyr(Me)arginine vasopressin for vasopressin-V1 blockade, and the L-NAME infusion was repeated. After 140 minutes of L-NAME infusion into the control group, mean arterial pressure and renal vascular resistance had increased 16% and 71%, and renal blood flow, glomerular filtration rate, urine flow, and urinary sodium excretion had decreased 33%, 16%, 61%, and 64%, respectively. The decrement in renal blood flow and glomerular filtration during L-NAME administration was unaffected by any of the neurohumoral blockers. During V1 blockade L-NAME resulted in only a 3% increase in arterial pressure, attenuation of the renal vascular resistance response, and almost total elimination of the decrease in urine flow. During angiotensin blockade the L-NAME-induced increase in arterial pressure was markedly attenuated, and the decrease in urinary sodium excretion was attenuated in the alpha 1 plus beta blockade group.(ABSTRACT TRUNCATED AT 250 WORDS)
Manning
R D
RD
Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson 39216-4505.
Hu
L
L
Williamson
T D
TD
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000037:antagonists & inhibitors; D001794:Blood Pressure; D002320:Cardiovascular Physiological Phenomena; D004231:Diuresis; D004285:Dogs; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000502:physiology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009318:Natriuresis; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000502:physiology*; D012079:Renal Circulation; D014655:Vascular Resistance
D001127:Arginine Vasopressin; D009569:Nitric Oxide; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester