title: Novel high-performance liquid chromatographic method using solid-phase on-line elution for determination of metolazone in plasma and whole blood.
abstract: A novel solid-phase on-line elution HPLC method employing fluorescence detection to measure metolazone in plasma and whole blood has been developed. The method is sensitive and selective for metolazone and linear over a dynamic range of 1-50 ng/ml with a sample requirement of 250 microliters. The limit of quantitation for the method is 1 ng/ml and combined intra- and inter-day accuracy and precision had an error and coefficient of variation of 2.9 and 5.5%, respectively.
authors.affiliation: Division of Clinical Pharmacology, Medical College of Virginia, Richmond 23298-0160.
authors.forename: D
authors.initials: D
authors.lastname: Farthing
authors.forename: D A
authors.initials: DA
authors.lastname: Sica
authors.forename: I
authors.initials: I
authors.lastname: Fakhry
authors.forename: T W
authors.initials: TW
authors.lastname: Gehr
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000188:drug therapy; D008297:Male; D008788:Metolazone / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D008788:Metolazone