Inhibition of platelet thromboxane formation and phosphoinositides breakdown by diisoeugenol.
Diisoeugenol inhibited the platelet aggregation and ATP release of rabbit platelets caused by ADP, arachidonic acid, platelet-activating factor (PAF), collagen and thrombin. Prolongation of the incubation time of platelets with diisoeugenol did not cause further inhibition and the aggregability of platelets could not be restored after washing. In human platelet-rich plasma, diisoeugenol inhibited the biphasic aggregation and ATP release induced by adrenaline and ADP in a concentration-dependent manner. Thromboxane B2 formation caused by arachidonic acid, collagen and thrombin was markedly inhibited by diisoeugenol in a concentration-dependent manner. Diisoeugenol also inhibited the formation of inositol monophosphate caused by collagen, PAF and thrombin. The cAMP level of washed platelets was not changed by diisoeugenol. It is concluded that the antiplatelet effect of diisoeugenol is due to the inhibition of thromboxane formation and phosphoinositides breakdown.
Lin
C H
CH
Pharmacological Institute, College of Medicine, National Taiwan University, Taipei.
Kuo
Y H
YH
Lin
Y L
YL
Teng
C M
CM
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis; D005054:Eugenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D013917:Thrombin / Q000494:pharmacology; D013931:Thromboxanes / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D008563:Membrane Lipids; D010716:Phosphatidylinositols; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D013931:Thromboxanes; D016718:Arachidonic Acid; D005054:Eugenol; C087580:diisoeugenol; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003094:Collagen; D000242:Cyclic AMP; D013917:Thrombin