Allele-specific DNA-protein interactions associated with the X-box regulatory region of the DQB1*0302 gene.
The X box is an essential transcriptional regulatory region for both constitutive and inducible expression of HLA-class II genes, and, while highly conserved among class II genes, both locus- and allele-specific polymorphisms exist. Using gel regardation analysis, we have analyzed the binding of B cell nuclear proteins to the X box regions of the DQB1*0302, *0301, and DRA genes and have identified two distinct X box binding complexes which differ for the diabetes-associated DQB1*0302 allele.
Shewey
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LM
Virginia Mason Research Center, Seattle, WA 98101.
Nepom
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D000483:Alleles; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002461:Cell Line, Transformed; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D004198:Disease Susceptibility / Q000276:immunology; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D015345:Oligonucleotide Probes; D011110:Polymorphism, Genetic; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D013379:Substrate Specificity; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D004247:DNA