title: Colocalization of octopamine and FMRFamide related peptide in identified heart projecting (DUM) neurones in the locust revealed by immunocytochemistry.
abstract: Immunocytochemical techniques are employed to reveal colocalization of octopamine with FMRFamide related peptide in the locust ventral nervous system. In each unfused pregenital abdominal ganglia (A4-A6) there are 3 octopamine-like immunoreactive neurones. By combining intracellular Lucifer yellow staining with subsequent immunocytochemistry these are individually identified as the efferent dorsal unpaired median (DUM) neurones DUM-1 and DUM-2, which innervate abdominal tergal and respectively sternal skeletal muscles, and DUM heart-1, an FMRFamide-like immunoreactive neurone which projects to the heart and associated alary muscles. Colocalization of octopamine- and FMRFamide-like immunoreactivity in DUM heart-1 is verified by alternate staining of consecutive sections. With respect to locust ventral ganglia, this investigation shows that colocalization of octopamine with an FMRFamide related peptide is restricted to a single DUM cell occurring in each abdominal ganglion 2-7, which most likely corresponds to segmental homologues of DUM heart-1.
authors.affiliation: Institut für Neurobiologie, Freie Universität Berlin, Germany.
authors.forename: P A
authors.initials: PA
authors.lastname: Stevenson
authors.forename: H J
authors.initials: HJ
authors.lastname: Pflüger
mesh_terms: D000005:Abdomen / Q000294:innervation; D000818:Animals; D005456:Fluorescent Dyes; D017952:Ganglia, Invertebrate / Q000166:cytology*; D006110:Grasshoppers; D006321:Heart / Q000294:innervation; D007150:Immunohistochemistry / Q000379:methods; D007447:Invertebrate Hormones / Q000032:analysis; D007546:Isoquinolines; D009132:Muscles / Q000294:innervation; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000032:analysis*; D009655:Octopamine / Q000032:analysis*; D009842:Oligopeptides / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D007447:Invertebrate Hormones; D007546:Isoquinolines; D009479:Neuropeptides; D009842:Oligopeptides; D009655:Octopamine; C017475:lucifer yellow; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide