Suitability of different protein A-gold markers for immunogold-silver staining in paraffin sections.
An incubation protocol to immunolabel Lowicryl semithin sections was applied to paraffin probes. To improve the labeling density, colloidal gold complexes of different preparations and sizes were compared. The type of colloidal gold preparation used was found to affect the specificity of the immunostaining. Gold colloid of 5 nm diameter particle size prepared with white phosphorus minimized nonspecific background labeling of beta-casein in paraffin embedded sections of the mammary epithelium of pregnant mice. Gold colloids of 5 nm and 9 nm diameter particle size prepared in varying concentrations of tannic acid generated significant nonspecific staining in similar tissue preparations.
Breter
H
H
Department of Electron Microscopy, Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.
Erdmann
B
B
D000818:Animals; D002364:Caseins / Q000032:analysis; D005260:Female; D017979:Gold Colloid / Q000032:analysis*; D006133:Growth Substances / Q000032:analysis; D007150:Immunohistochemistry; D008321:Mammary Glands, Animal / Q000033:anatomy & histology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016612:Paraffin Embedding; D010316:Particle Size; D011247:Pregnancy; D013205:Staphylococcal Protein A / Q000032:analysis*
D002364:Caseins; D017979:Gold Colloid; D006133:Growth Substances; D013205:Staphylococcal Protein A