Screening method for the determination of volatiles in biomedical samples by means of an off-line closed-loop trapping system and high-resolution gas chromatography-ion trap detection.
A method is described for the analysis of volatile organic compounds in biological matrices (faeces and urine). The technique is based on off-line preconcentration by means of a closed-loop trapping system followed by high-resolution gas chromatography-ion trap detection (HRGC-ITD) for separation and identification of the compounds. The technique has been validated for pattern recognition in faecal and urine samples from healthy volunteers. It is considered a very promising tool in metabolic research.
Ghoos
Y
Y
Department of Gastrointestinal Research, University Hospital Gasthuisberg, K.U. Leuven, Belgium.
Claus
D
D
Geypens
B
B
Hiele
M
M
Maes
B
B
Rutgeerts
P
P
D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D005243:Feces / Q000737:chemistry*; D005285:Fermentation; D006801:Humans; D006838:Hydrocarbons / Q000032:analysis*; D007202:Indicators and Reagents; D008055:Lipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011786:Quality Control; D014556:Urine / Q000737:chemistry*
D002241:Carbohydrates; D006838:Hydrocarbons; D007202:Indicators and Reagents; D008055:Lipids; D011506:Proteins