Characterization of haemolyser-resistant cells increased in the blood of erythropoietin-treated mice.
Recently, we reported a new in vivo assay method for erythropoietin (Epo) by means of counts of the number of haemolyser-resistant cells (HRCs) increased in Epo-injected mice. Here, we attempted to characterize the HRCs. Flow-cytometric studies revealed that HRCs obtained from Epo-injected mice expressed the transferrin receptor on their surface membranes. Furthermore, a fluorophotometric study suggested that the number of transferrin receptor-positive cells increased in a dose-dependent manner in response to treatment with Epo. On the other hand, flowcytometric and fluorophotometric studies of glycophorin A on HRCs using a rabbit antiglycophorin A antibody also showed a high expression of glycophorin A on them as compared with on HRCs from vehicle-treated animals (control). The results indicated that HRCs could be defined by their expression of both transferrin receptors and glycophorin A. We concluded that HRCs might be immature reticulocytes.
Said
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AA
National Institute of Health Sciences (formerly National Institute of Hygienic Sciences), Tokyo, Japan.
Yamaguchi
T
T
Uchida
E
E
Hayakawa
T
T
D000818:Animals; D001681:Biological Assay; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D006021:Glycophorins / Q000032:analysis; D006461:Hemolysis / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D011990:Receptors, Transferrin / Q000032:analysis; D012156:Reticulocytes / Q000187:drug effects
D006021:Glycophorins; D011990:Receptors, Transferrin; D004921:Erythropoietin