title: Temperature-dependent change of serological specificity of Candida albicans NIH A-207 cells cultured in yeast extract-added Sabouraud liquid medium: disappearance of surface antigenic factors 4, 5, and 6 at high temperature.
abstract: The cells of Candida albicans NIH A-207 strain (A-strain) cultivated in YSLM at high temperatures (37 and 40 degrees C) did not undergo agglutination with the factor sera 4, 5, and 6 in a commercially available factor serum kit, 'Candida Check', and formed a grape-like shape. The mannans isolated from the cells had lost their reactivity against the factor sera in ELISA. It was also revealed by 1H NMR analysis that the mannans contained neither a phosphate group nor a beta-1,2-linked mannopyranose unit, although these mannans increased the non-reducing terminal alpha-1,3-linked mannopyranose unit. The cells and the mannans prepared by cultivation at such high temperatures followed by 27 degrees C in the same medium entirely recovered the reactivity with the factor sera.
authors.affiliation: Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okawa
authors.forename: T
authors.initials: T
authors.lastname: Takahata
authors.forename: M
authors.initials: M
authors.lastname: Kawamata
authors.forename: M
authors.initials: M
authors.lastname: Miyauchi
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000372:Agglutination Tests; D000946:Antigens, Fungal / Q000032:analysis / Q000378:metabolism*; D002176:Candida albicans / Q000145:classification / Q000254:growth & development*; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis*; D012441:Saccharomyces cerevisiae; D013696:Temperature
chemical_list: D000946:Antigens, Fungal; D003470:Culture Media; D008351:Mannans