The analysis of polypeptides in the nuclei and cytoplasm of cells infected with murine herpesvirus 72.
Twenty-six polypeptides in murine herpesvirus isolate 72 (MHV-72) were identified and the synthesis and accumulation of 21 virus-specific polypeptides in Vero cells during the course of productive infection with MHV-72 were examined. Five of the infected cell polypeptides accumulated within the nuclei and nine accumulated within the cytoplasm of MHV-72-infected cells. Seven polypeptides were identified within the nuclei and cytoplasm in equivalent amounts. The major capsid protein was shown to have an M(r) of 161K. Thirteen virus-specific polypeptides were solubilized with Nonidet P-40 or radioimmunoprecipitation assay buffer and immunoprecipitated with rabbit and mouse immune sera. Analysis of the polypeptides of MHV-72 indicate a closer resemblance between MHV-72 and the gammaherpesviruses herpesvirus saimiri and Epstein-Barr virus than with the alphaherpesvirus herpes simplex virus type 1.
Reichel
M
M
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Matis
J
J
Mistrikova
M
M
Lesso
J
J
D000818:Animals; D002213:Capsid / Q000737:chemistry*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006564:Herpesviridae / Q000737:chemistry*; D006566:Herpesviridae Infections / Q000382:microbiology*; D008970:Molecular Weight; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry*
D014764:Viral Proteins