title: Directed disruption of the Chlamydomonas chloroplast psbK gene destabilizes the photosystem II reaction center complex.
abstract: Using particle gun-mediated chloroplast transformation we have disrupted the psbK gene of Chlamydomonas reinhardtii with an aadA expression cassette that confers resistance to spectinomycin. The transformants are unable to grow photoautotrophically, but they grow normally in acetate-containing medium. They are deficient in photosystem II activity as measured by fluorescence transients and O2 evolution and they accumulate less than 10% of wild-type levels of photosystem II as measured by immunochemical means. Pulse-labeling experiments indicate that the photosystem II complex is synthesized normally in the transformants. These results differ from those obtained previously with similar cyanobacterial psbK mutants that were still capable of photoautotrophic growth (Ikeuchi et al., J. Biol. Chem. 266 (1991) 1111-1115). In C. reinhardtii the psbK product is required for the stable assembly and/or stability of the photosystem II complex and essential for photoautotrophic growth. The data also suggest that the stability requirements of the photosynthetic complexes differ considerably between C. reinhardtii and cyanobacteria.
authors.affiliation: Graduate School of Natural Science and Technology, Okayama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Goldschmidt-Clermont
authors.forename: J D
authors.initials: JD
authors.lastname: Rochaix
mesh_terms: D020418:Algal Proteins; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002736:Chloroplasts / Q000502:physiology; D046911:Macromolecular Substances; D016296:Mutagenesis; D010788:Photosynthesis / Q000502:physiology*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics* / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000235:genetics*; D015800:Protozoan Proteins; D014170:Transformation, Genetic
chemical_list: D020418:Algal Proteins; D046911:Macromolecular Substances; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D015800:Protozoan Proteins