[The characteristic of the spindle fiber attachment (SFA) enriched fraction from mouse nuclei].
The characteristics of the particulate mouse centromere enriched fraction from isolated nuclei obtained in our laboratory were investigated by indirect immunofluorescence, test of the activity of microtubule organizing center (MTOC), SDS-PAGE, and fluorescence in situ hybridization. Most of the particles of the fraction are complexes of DNA and kinetochore proteins and show MTOC activity. The DNA isolated from the fraction hybridizes the DNA in the regions of the primary constrictions of all chromosomes of ascite cells. The kinetochore proteins of the proteins isolated from the fraction are mainly those with molecular weight of 55 and 59 KD. It is evident that the fraction obtained is a centromere enriched nuclear fraction as indicated in our previous report.
Ni
Z M
ZM
Shanghai Institute of Cell Biology, Academic Sinica, China.
Yi
N
N
Chou
Y
Y
Shi
L J
LJ
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology; D002467:Cell Nucleus; D002503:Centromere; D004247:DNA / Q000302:isolation & purification; D051379:Mice; D008868:Microtubule Proteins; D008870:Microtubules; D009687:Nuclear Proteins / Q000502:physiology*; D008941:Spindle Apparatus / Q000737:chemistry
D008868:Microtubule Proteins; D009687:Nuclear Proteins; D004247:DNA