Fluorescence-activated cell sorting-derived clones of Babesia bigemina show karyotype polymorphism.
Use of the fluorescence-activated cell sorter proved to be an accurate and highly efficient means for cloning Babesia parasites. These qualities were examined by separating a mixed population of Babesia-infected bovine erythrocytes composed of two isolates with different karyotypes. Direct evidence of polymorphism was detected during comparison of the resultant clones.
Estes
D M
DM
Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
Bailey
C W
CW
Barnett
L
L
Lafrenz
D
D
Brandt
H M
HM
Jensen
J B
JB
Allen
G K
GK
Carson
C A
CA
D000818:Animals; D001403:Babesia / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D016172:DNA Fingerprinting; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry / Q000379:methods; D007621:Karyotyping; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D017931:DNA Primers; D016054:DNA, Protozoan