Analysis of the inducer-responsive CAR1 upstream activation sequence (UASI) and the factors required for its operation.
Induced production of arginase (CAR1) enzyme activity and steady-state CAR1 mRNA in Saccharomyces cerevisiae requires wild-type ARG80/ARGRI and ARG81/ARGRII gene products. We demonstrate here that these gene products, along with that of the MCM1 gene, are required for the inducer-dependent USAI-A, UASI-B and UASI-C elements to function but they are not required for operation of inducer-independent CAR1 UASC1 or UASC2. Through the use of single and multiple point mutations, the CAR1 UASI-B and UASI-C elements were demonstrated to be at least 23 bp in length. Moreover, simultaneous mutation of both ends of an elements gave stronger phenotypes than mutations at either end. The center of the element was more sensitive to mutation than were the ends.
Kovari
L Z
LZ
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Fourie
M
M
Park
H D
HD
Kovari
I A
IA
Van Vuuren
H J
HJ
Cooper
T G
TG
D001119:Arginase / Q000235:genetics*; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D017930:Genes, Reporter; D007763:Lac Operon; D026341:Minichromosome Maintenance 1 Protein; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D026341:Minichromosome Maintenance 1 Protein; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D001119:Arginase