Development of a nested-PCR test based on sequence analysis of epizootic hemorrhagic disease viruses non-structural protein 1 (NS1).
Two orbiviruses, epizootic hemorrhagic disease (EHD) and bluetongue (BTV) viruses, cause disease in domestic and wild ruminant species. The gene that encodes non-structural protein 1 (NS1) of EHD virus, serotype 1, was sequenced and compared to EHD and BTV NS1 sequences. The NS1 gene was found to be more conserved than the VP3 gene, and was selected as a target for polymerase chain reaction (PCR) amplification. The NS1 genes of several BTV viruses and another orbivirus, African horse sickness (AHS), were compared to the EHD NS1 genes. This information was used to develop a capture nested-PCR for detection and differentiation of EHD from BTV viral RNA.
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United States Department of Agriculture, Arthropod-borne Animal Diseases Research Laboratory, Laramie, WY 82071-3965.
D000356:African Horse Sickness Virus / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D001820:Bluetongue virus / Q000235:genetics; D018164:Hemorrhagic Disease Virus, Epizootic / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics*
C079340:NS1 protein, Dengue virus type 2; C078966:NS1 protein, bluetongue virus; D017361:Viral Nonstructural Proteins