Monoclonal IgM antibodies to GM1 and asialo-GM1 in chronic neuropathies cross-react with Campylobacter jejuni lipopolysaccharides.
We tested monoclonal IgM anti-GM1 and asialo-GM1 antibodies from 6 patients with chronic motor neuropathies for binding to lipopolysaccharides (LPS) from three stains of Campylobacter jejuni. Four of the 6 patients showed strong reactivity with LPS from at least one of the three C. jejuni strains tested as shown by enzyme-linked immunosorbent assay or western blot. Preabsorption with GM1 or asialo-GM1, or blocking with cholera toxin, prevented antibody binding to LPS. These studies indicate that human anti-GM1 or anti-asialo-GM1 antibodies cross-react with LPS from certain strains of C. jejuni, and that bacterial LPS might provide antigenic stimuli for the activation of B cells expressing anti-GM1 antibodies.
Wirguin
I
I
Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Suturkova-Milosevic
L
L
Della-Latta
P
P
Fisher
T
T
Brown
R H
RH
Latov
N
N
D000911:Antibodies, Monoclonal / Q000097:blood*; D010367:Arachis; D016123:Campylobacter jejuni / Q000276:immunology*; D002772:Cholera Toxin; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D037102:Lectins; D008070:Lipopolysaccharides / Q000276:immunology*; D016472:Motor Neuron Disease / Q000097:blood / Q000276:immunology*; D010265:Paraproteinemias / Q000097:blood / Q000276:immunology*; D019887:Peanut Agglutinin; D037121:Plant Lectins; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D006017:Glycolipids; D007075:Immunoglobulin M; D037102:Lectins; D008070:Lipopolysaccharides; D019887:Peanut Agglutinin; D037121:Plant Lectins; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D002772:Cholera Toxin