Enzyme electrophoresis, sero- and subtyping, and outer membrane protein characterization of two Neisseria meningitidis strains involved in laboratory-acquired infections.
Two cases of laboratory-acquired infections due to Neisseria meningitidis were suspected to have occurred in two French hospitals. The first case occurred shortly, i.e., 3 days, after one strain had been handled by a laboratory technician, and the link between this strain and the strain causing meningitis was easily established. In the second case, infection occurred 3 weeks after 10 strains had been handled by a technician. In this case, it was necessary to use high-resolution markers in order to establish the link between the infecting strain and 1 of the 10 strains handled. The antigenic formulae of the two infecting strains (serogroup:serotype:subtype) were, respectively, C:NT:P1.12 and B:2a:P1.2. Outer membrane protein profile analysis and multilocus enzyme electrophoresis unequivocally confirmed the identity of the respective strains.
Guibourdenche
M
M
Centre National de Référence des Méningocoques et Neisseria apparentées (CNRM), Institut Pasteur, Paris, France.
Darchis
J P
JP
Boisivon
A
A
Collatz
E
E
Riou
J Y
JY
D000328:Adult; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification; D004798:Enzymes / Q000235:genetics / Q000302:isolation & purification; D005602:France; D005838:Genotype; D006801:Humans; D007757:Laboratory Infection / Q000382:microbiology*; D008585:Meningitis, Meningococcal / Q000382:microbiology*; D009345:Neisseria meningitidis / Q000737:chemistry / Q000145:classification / Q000201:enzymology*; D012703:Serotyping
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004798:Enzymes