Structural proteins of mycobacteriophage I3: cloning, expression and sequence analysis of a gene encoding a 70-kDa structural protein.
The structural proteins of mycobacteriophage I3 have been analysed by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE), radioiodination and immunoblotting. Based on their abundance the 34- and 70-kDa bands appeared to represent the major structural proteins. Successful cloning and expression of the 70-kDa protein-encoding gene of phage I3 in Escherichia coli and its complete nucleotide sequence determination have been accomplished. A second (partial) open reading frame following the stop codon for the 70-kDa protein was also identified within the cloned fragment. The deduced amino-acid sequence of the 70-kDa protein and the codon usage patterns indicated the preponderance of codons, as predicted from the high G+C content of the genomic DNA of phage I3.
Ramesh
G R
GR
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Gopinathan
K P
KP
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D003062:Codon; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009160:Mycobacteriophages / Q000737:chemistry* / Q000235:genetics*; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*
D003062:Codon; D004279:DNA, Viral; D015678:Viral Structural Proteins