Regional mapping of the gene encoding dihydroorotate dehydrogenase, an enzyme involved in UMP synthesis, electron transport, and superoxide generation, to human chromosome region 16q22.
De novo UMP synthesis is a critical metabolic pathway for nucleic acid synthesis and for a variety of metabolic pathways. The pathway is a target for many widely used cancer chemotherapy agents, several of which are pyrimidine analogs. Humans and cattle have been described with mutations in UMP synthesis that lead to serious inborn errors of metabolism. Dihydroorotate dehydrogenase (EC 1.3.3.1) (DHODH) carries out the fourth committed step in the pathway and may also be important for mitochondrial electron transport and oxygen radical metabolism. We report here that the gene encoding this enzyme in humans is located in the chromosomal region 16q22. With the mapping of DHODH, the mapping of all the steps of UMP synthesis is complete. All three genes involved map to different human chromosomes. This information is important in consideration of regulation of UMP synthesis in mammals, including humans.
Barnes
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Eleanor Roosevelt Institute, Denver, Colorado 80206.
Parry
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P
Hart
I
I
Jones
C
C
Minet
M
M
Patterson
D
D
D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002885:Chromosomes, Human, Pair 16; D017931:DNA Primers; D000090722:Dihydroorotate Dehydrogenase; D004579:Electron Transport; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D016133:Polymerase Chain Reaction; D013481:Superoxides / Q000378:metabolism; D014542:Uridine Monophosphate / Q000096:biosynthesis
D017931:DNA Primers; D000090722:Dihydroorotate Dehydrogenase; D013481:Superoxides; D014542:Uridine Monophosphate; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors