Changes in immunostaining of cochleas with experimentally induced endolymphatic hydrops.
Cochleas with experimentally induced endolymphatic hydrops were immunostained for Na+,K(+)-ATPase, intracellular Ca(++)-ATPase, carbonic anhydrase, aldehyde dehydrogenase, calcium-binding proteins, vimentin, and the gap junction protein, connexin 26. No changes in immunostaining of hydropic ears were observed 1 week after blockage of the endolymphatic duct. Two weeks to 1 month after the operation, immunostaining of type I fibrocytes in the spiral ligament, which are positive for all but Na+,K(+)-ATPase, was slightly decreased on the operated side. These changes became more pronounced 3 months after the operation. However, staining for Na+,K(+)-ATPase of the stria vascularis and of type II fibrocytes of the spiral ligament was not reduced until 6 months postoperative. The reduction of enzymes and other cell constituents that may be involved in ion balance of cochlear fluids indicates that cells in the spiral ligament play an important role in cochlear homeostasis and that they merit further study in animal and human otopathology.
Ichimiya
I
I
Department of Otolaryngology, Oita Medical University, Japan.
Adams
J C
JC
Kimura
R S
RS
D000251:Adenosine Triphosphatases / Q000032:analysis; D000444:Aldehyde Dehydrogenase / Q000032:analysis; D000818:Animals; D002147:Calmodulin / Q000032:analysis; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D002256:Carbonic Anhydrases / Q000032:analysis; D003051:Cochlea / Q000737:chemistry*; D000072259:Connexin 26; D017630:Connexins / Q000032:analysis; D018159:Endolymphatic Hydrops / Q000378:metabolism*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D009418:S100 Proteins / Q000032:analysis; D013997:Time Factors; D014746:Vimentin / Q000032:analysis
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D017630:Connexins; D009418:S100 Proteins; D014746:Vimentin; D000072259:Connexin 26; D000444:Aldehyde Dehydrogenase; D000251:Adenosine Triphosphatases; D002256:Carbonic Anhydrases