title: Sympathoadrenal regulation of adrenal androstenedione release.
abstract: The effects of epinephrine and of splanchnic nerve activation on adrenocortical androstenedione release were studied in intact isolated perfused pig adrenals with preserved nerve supply. In addition, long-term effects of epinephrine were characterized in bovine adrenocortical cells in primary culture. To investigate the contact zones of the androgen-producing cells of the zona reticularis with the catecholamine producing cells of the adrenal medulla, cortical cells were immunostained for cytochrome P450 side chain cleavage (P450SCC). Perfusion of the isolated adrenals with epinephrine (10(-7) to 10(-5) M) stimulated androstenedione release in a dose-dependent manner. At a concentration of 10(-6) M, epinephrine provoked an increase to 179.11 +/- 16.14% of basal secretion (p < 0.05). Electrical stimulation of the splanchnic nerves led to an increase to 151.5 +/- 9.24% of basal values (p < 0.05). Epinephrine (10(-6) M) reached 40% and activation of the splanchnic nerves 26% of the stimulatory effect of ACTH at a physiological concentration (10(-10) M). The alpha-agonist phenylephrine had no effect on androstenedione release. In cell cultures, epinephrine stimulated the release of androstenedione in a dose-dependent manner with an ED50 of 0.75 x 10(-6) M. The maximal effect was reached at 10(-5) M with 8.92 +/- 0.66 pmol androstenedione/dish/24 h; the basal secretion was 1.44 +/- 0.54 pmol/dish/24 h. The epinephrine-stimulated androstenedione release was abolished by the beta-adrenergic antagonist propranolol while the alpha-adrenergic antagonist phentolamine had no effect. Immunohistochemical staining of paraffin sections of bovine and porcine adrenals for P450SCC revealed that zona reticularis and zona medullaris are closely interwoven.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Abteilung für Innere Medizin I, Medizinische Klinik und Poliklinik, Universität Ulm, BRD.
authors.forename: M
authors.initials: M
authors.lastname: Ehrhart-Bornstein
authors.forename: S R
authors.initials: SR
authors.lastname: Bornstein
authors.forename: H
authors.initials: H
authors.lastname: Güse-Behling
authors.forename: H G
authors.initials: HG
authors.lastname: Stromeyer
authors.forename: T N
authors.initials: TN
authors.lastname: Rasmussen
authors.forename: W A
authors.initials: WA
authors.lastname: Scherbaum
authors.forename: G
authors.initials: G
authors.lastname: Adler
authors.forename: J J
authors.initials: JJ
authors.lastname: Holst
mesh_terms: D000311:Adrenal Glands / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000735:Androstenedione / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004558:Electric Stimulation; D004837:Epinephrine / Q000494:pharmacology; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D013552:Swine; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D002395:Catecholamines; D000735:Androstenedione; D000324:Adrenocorticotropic Hormone; D003577:Cytochrome P-450 Enzyme System; D011433:Propranolol; D004837:Epinephrine; D010646:Phentolamine