title: Thermal resistance of partially purified proteinase of Pseudomonas fluorescens P-26.
abstract: Heat resistance of the Pseudomonas fluorescens P-26 proteinase in terms of D-value was studied in whole milk, skim milk, whey and 0.05 mol l-1 phosphate buffer at 72.5, 130, 135, 140, 145 and 150 degrees C subsequent to its partial purification through (NH4)2 SO4 precipitation (45-65% saturation) and solvent fractionation with 1.0 to 2.0 volumes of isopropanol. The D-value was maximum for the proteinase at all temperatures when determined in whole milk (D150 = 0.088).
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Uplacksh
authors.forename: D K
authors.initials: DK
authors.lastname: Mathur
authors.forename: R K
authors.initials: RK
authors.lastname: Malik
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D011232:Chemical Precipitation; D010450:Endopeptidases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D006358:Hot Temperature; D008892:Milk; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D012997:Solvents
chemical_list: D012997:Solvents; D010450:Endopeptidases; D000645:Ammonium Sulfate