Lymphocyte expression in transgenic trout by mouse immunoglobulin promoter/enhancer.
Two groups of transgenic rainbow trout (Oncorhynchus mykiss, Walbaum) have been produced and compared. One group harbored the reporter gene of chloramphenicol acetyltransferase (CAT) associated with mouse immunoglobulin (Ig) promoter/enhancer (pUCL-CAT-E). The other group carried the same reporter gene under the control of the cytomegalovirus promoter/enhancer (pCMV-CAT). Slot blot analysis of DNA from blood cells and other tissues from pUCL-CAT-E fish showed variation of copy number between the major tissues but not between red and white blood cells. Southern blot analysis indicated that multiple copies organized in concatemers were incorporated into the genome. The pCMV-CAT fish had a pronounced expression of CAT in both white and red blood cells. In contrast, activity of CAT was found in the white blood cells of all pUCL-CAT-E fish but not in their red blood cells. Expression in white blood cells was found preferentially in sIg+ cells, indicating that B cells are the major expressors. High expression was also found in spleen and kidney, but the activity found in thymocytes was equal to the background level. Analysis of some major tissues showed high white blood cell expression associated with low tissue expression, except that liver (known to contain lymphoid tissue in fish) was higher. Thus the regulatory elements of the Ig gene from mouse induce a tissue-specific expression in fish.
Michard-Vanhée
C
C
Laboratoire de Génétique des Poissons, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Chourrout
D
D
Strömberg
S
S
Thuvander
A
A
Pilström
L
L
D000818:Animals; D030801:Animals, Genetically Modified; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D005803:Genes, Immunoglobulin / Q000235:genetics*; D017930:Genes, Reporter; D008214:Lymphocytes / Q000276:immunology*; D008221:Lymphoid Tissue / Q000196:embryology; D051379:Mice / Q000235:genetics*; D017686:Oncorhynchus mykiss / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D014018:Tissue Distribution
D015500:Chloramphenicol O-Acetyltransferase