Acetylcholinesterase activity is associated with efferent endings in the sensory epithelia of the utricle and semicircular canals of the rainbow trout inner ear.
The mechanosensory hair cells of the utricle and semicircular canals of the trout inner ear are morphologically similar to type II hair cells of the avian and mammalian vestibular end organs. These cells are innervated by two types of nerve terminals. The nonvesiculated terminals are considered to be afferent, and the vesiculated endings are presumed to be efferent. The presumptive efferent endings contain numerous clear, round vesicles and a few that are dense-cored. Histocytochemical, electron microscopic analysis has localized acetylcholinesterase activity to plasma membranes of vesiculated, presumptive efferent nerve terminals in sensory epithelia of the utricle and semicircular canals. No reaction product was observed at the receptoneural synapse or found in nonvesiculated, presumptive afferent endings. Control specimens incubated in the presence of eserine sulfate, an inhibitor of acetylcholinesterase, were devoid of reaction product. These results support the tenet that vesiculated nerve endings in the sensory epithelia of the utricle and semicircular canals of the trout are cholinergic.
Khan
K M
KM
Department of Otolaryngology, Wayne State University, Detroit, MI 48201.
Drescher
M J
MJ
Hatfield
J S
JS
Drescher
D G
DG
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004525:Efferent Pathways / Q000201:enzymology; D004848:Epithelium; D005260:Female; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000201:enzymology*; D017686:Oncorhynchus mykiss; D012444:Saccule and Utricle / Q000502:physiology*; D012665:Semicircular Canals / Q000502:physiology*; D012677:Sensation / Q000502:physiology*; D014018:Tissue Distribution
D000110:Acetylcholinesterase