Cytokine synthesis and apoptosis by intestinal intraepithelial lymphocytes: signaling of high density alpha beta T cell receptor+ and gamma delta T cell receptor+ T cells via T cell receptor-CD3 complex results in interferon-gamma and interleukin-5 production, while low density T cells undergo DNA fragmentation.
To study the biological consequences of cytokine production and apoptosis by intraepithelial lymphocytes (IEL), we have studied these characteristics in both the high and low density CD3+ IEL populations. Stimulation of low- or high-density CD3+ IEL via the T cell receptor (TCR)-CD3 complex using monoclonal anti-CD3, anti-alpha beta TCR or anti-gamma delta TCR antibodies resulted in opposing effects. In one case, a significant number of the high-density CD3+ T cells entered cell cycle from the resting stage (DNA replication was observed) and anti-TCR-CD3 treatment enhanced the numbers of interferon-gamma and interleukin-5 spot-forming cells in this cell fraction. In contrast, when the low-density alpha beta TCR+ or gamma delta TCR+ T cells were activated via the TCR-CD3 complex, DNA fragmentation was observed. These results demonstrated that the activation signals transduced via the TCR-CD3 complex resulted in their entry into the cell cycle and subsequent interferon-gamma and interleukin-5 production in the high-density IEL T cell subset. However, identical signals induced apoptosis in the majority of the low-density fraction of CD3+ IEL.
Yamamoto
M
M
Department of Oral Biology, University of Alabama Center.
Fujihashi
K
K
Amano
M
M
McGhee
J R
JR
Beagley
K W
KW
Kiyono
H
H
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D017252:CD3 Complex / Q000502:physiology; D002453:Cell Cycle; D016207:Cytokines / Q000096:biosynthesis*; D004261:DNA Replication; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007371:Interferon-gamma / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000502:physiology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000502:physiology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*
D017252:CD3 Complex; D016207:Cytokines; D015848:Interleukin-5; D017260:Receptor-CD3 Complex, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007371:Interferon-gamma