Detection of bcrabl fusion mRNA in samples of whole, unfractionated blood.
Using a new strategy, capture-RT-PCR, bcrabl fusion mRNA sequences were specifically and sensitively detected in samples of whole blood from leukemia patients with the Philadelphia chromosome. Sample processing required only mixing blood with the chaotropic salt, GuSCN, and mRNA was captured during a short incubation of prepared blood with an affinity membrane. Immobilized mRNA sequences were amplified without elution. No radioisotopes or Southern transfer were needed.
Kolbe
T
T
Department of Neoplastic Diseases, Hahnemann University, Philadelphia, PA 19102-1192.
Cuddy
K
K
Brodsky
I
I
Marks
D I
DI
Gillespie
D
D
D001800:Blood Specimen Collection / Q000379:methods; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis*; D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000097:blood*
D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl