Visualization of RNA tertiary structure by RNA-EDTA.Fe(II) autocleavage: analysis of tRNA(Phe) with uridine-EDTA.Fe(II) at position 47.
To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA.Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)2(SO4)2 and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.
Han
H
H
Beckman Institute, California Institute of Technology, Pasadena 91125.
Dervan
P B
PB
D001483:Base Sequence; D004492:Edetic Acid / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012360:RNA, Transfer, Phe / Q000032:analysis / Q000737:chemistry*; D014529:Uridine / Q000737:chemistry
D005296:Ferrous Compounds; D012360:RNA, Transfer, Phe; D004492:Edetic Acid; D014529:Uridine