Simultaneous evaluation of vitamins A and E in human plasma by normal phase HPLC.
The production of toxic oxidants such as H2O2; OH. and O2.- which follows aerobic metabolism is highly enhanced in aging, alcohol consumption and in a variety of degenerative diseases including neoplasias. Among the different antioxidant agents that in human organisms counterbalance the oxidative stress, which damages lipid, protein and DNA, are included beta-carotene, alpha-tocopherol, glutathione, ascorbate, ubiquinol and specific enzymes such as superoxide dismutase, catalase and glutathione peroxidase. In particular, vitamins A and E are the most important lipid-soluble antioxidants in human tissues protecting polyunsaturated fatty acid from peroxidation. In this paper we report an improved method, obtained after modification of a normal phase HPLC method, which allows the simple and rapid simultaneous evaluation of vitamins A and E in plasma. The separation of vitamins is achieved by eluting the HPLC column with a linear gradient from 1 to 5% isopropanol in n-hexane. The eluate is simultaneously monitored by U.V. and fluorescence detection to identify and quantify vitamins A and E, respectively.
Belisario
M A
MA
Dipartimento di Scienze Animali, Università del Molise, Campobasso.
Azar
G
G
Oriani
G
G
Pizzuti
G P
GP
Sacchetti
L
L
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012016:Reference Values; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
D014801:Vitamin A; D014810:Vitamin E