In situ hybridization analysis of cytokine, proto-oncogene and tumour suppressor gene expression in psoriasis.
The purpose of this study was to investigate and to compare, by in situ hybridization, gene expression of IL-1 beta, IL-8, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-alpha, p53 and c-myc in lesions and in non-involved skin of patients with psoriasis. All lesional skin biopsies showed overexpression of IL-1 beta, IL-8 TGF-alpha mRNAs. IL-1 beta hybridization signals were strong in a small number of cells localized predominantly in the dermal papillae and in the suprapapillary epidermis. Overexpression of TGF-alpha was observed in all suprabasal keratinocytes, whereas strongly elevated IL-8 mRNA expression was found to be restricted to clusters of suprabasal keratinocytes. TGF-beta 3, p53 and c-myc transcripts were clearly detected in the epidermis of all biopsies, although expression levels were comparable in lesional and non-lesional skin.
Schmid
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P
CIBA-GEIGY Ltd., Pharma Division, Basel, Switzerland.
Cox
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D
McMaster
G K
GK
Itin
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P
D016207:Cytokines / Q000235:genetics*; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D016259:Genes, myc; D016158:Genes, p53; D006801:Humans; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D011565:Psoriasis / Q000235:genetics* / Q000276:immunology; D016211:Transforming Growth Factor alpha / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics
D016207:Cytokines; D007375:Interleukin-1; D016209:Interleukin-8; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta