Characterization of dual HIV-1 and HIV-2 serological profiles by polymerase chain reaction.
OBJECTIVE
To evaluate the frequency of dual HIV-1 and HIV-2 DNA sequences in patients with dual serological profiles.


METHODS
We tested 40 samples from AIDS patients living in Abidjan, Côte d'Ivoire.


METHODS
Dual serological reactivity was determined by double Western blot and two enzyme-linked immunosorbent assays with recombinant proteins and synthetic peptides as antigens. The Western blot was considered to show dual reactivity when sera reacted with at least two glycoproteins and one core protein of each virus. HIV DNA sequences were detected by hybridization to radiolabelled probes of polymerase chain reaction (PCR) products amplified using specific primers.


RESULTS
Both HIV-1 and HIV-2 DNA sequences were detected in four out of 11 samples with a dual serological profile and in four out of 24 samples with anti-HIV-1 antibodies only.


CONCLUSIONS
These results show that dual HIV-1 and HIV-2 serological profiles are not always due to infection by both viruses, and emphasize the need for a combination of serological and PCR assays for the appraisal of these viral infections.
Léonard
G
G
Laboratoire de Virologie, CHU Dupuytren, Limoges, Paris, France.
Chaput
A
A
Courgnaud
V
V
Sangaré
A
A
Denis
F
F
Brechot
C
C
D015492:AIDS Serodiagnosis; D001483:Base Sequence; D015153:Blotting, Western; D007560:Cote d'Ivoire / Q000453:epidemiology; D004279:DNA, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D015658:HIV Infections / Q000453:epidemiology / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D015498:HIV-2 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004279:DNA, Viral