Inhibition of phagocytosis of human macrophages induced by airborne particulates.
Human monocyte-derived macrophages isolated from peripheral blood were treated with different extracts of airborne particulates collected in the highly industrialized Rhine-Ruhr area. All tested extracts showed a substantial impairment of phagocytosis by inhibition of phagocytic activity as well as phagocytic capacity, while cell viability was rather well maintained. Significant reduction of phagocytosis already appeared at a concentration equivalent to extracted particulates from 3.8 m3 air. Having properties of alveolar macrophages, human monocyte-derived macrophage cultures may offer a reliable in vitro model for assessment of pulmonary toxicity by respirable pollutants.
Hadnagy
W
W
Institute of Hygiene, Heinrich-Heine-University Düsseldorf, Germany.
Seemayer
N H
NH
D000280:Administration, Inhalation; D000393:Air Pollutants / Q000633:toxicity*; D002478:Cells, Cultured; D006801:Humans; D007221:Industry; D008168:Lung / Q000098:blood supply; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000502:physiology*; D008853:Microscopy; D008954:Models, Biological; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects*; D012621:Seasons
D000393:Air Pollutants