title: [Use of a modified "antigen-capture" enzyme immunoassay for the identification of virus persistence in cattle infected with bovine virus diarrhea (BVD)].
abstract: Bovine viral diarrhoea (BVD)/mucosal disease (MD) is an economically important infectious disease of cattle, and persistently infected animals are of central epidemiological significance. The identification of these animals is a prerequisite for further sanitary measures in affected herds. In order to process large numbers of samples an antigen capture enzyme immunoassay (AC-ELISA) for the detection of bovine viral diarrhoea viral antigen in peripheral blood leukocytes of cattle of all ages has been developed. In this communication an improved method for the preparation of samples is described. A total of 563 blood samples was tested using the AC-ELISA and a routine virus isolation procedure on susceptible bovine cells. When compared to virus isolation in cell culture, the sensitivity of the AC-ELISA was 97%. From the samples that were virus negative in cell culture, 99.1% were also negative in the AC-ELISA. There is some evidence that the AC-ELISA discriminated between transiently and persistently infected animals.
authors.affiliation: Institut für Virologie, Tierärztlichen Hochschule Hannover.
authors.forename: I
authors.initials: I
authors.lastname: Greiser-Wilke
authors.forename: H R
authors.initials: HR
authors.lastname: Frey
authors.forename: J
authors.initials: J
authors.lastname: Böttcher
authors.forename: B
authors.initials: B
authors.lastname: Liess
authors.forename: V
authors.initials: V
authors.lastname: Moennig
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis / Q000382:microbiology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D007962:Leukocytes / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000662:veterinary
chemical_list: D000956:Antigens, Viral
vernacular_title: Anwendung eines modifizierten "Antigen-Capture"-Enzym-Immuntests zur Identifizierung persistent mit dem Virus der bovinen Virusdiarrhö (BVD) infizierter Rinder.