Effect of Artocarpus integer lectin on functional activity of guinea-pig complement.
The effect of Artocarpus integer lectin (lectin C) on the functional activity of guinea-pig complement was investigated. Purified and crude extract of lectin C from six cultivars of Artocarpus integer seeds were found to consume complement and thus decreased the complement-induced haemolytic activity of sensitized sheep erythrocytes. The change in the complement-mediated haemolytic activity was significantly decreased when incubation of the lectins was performed in the presence of melibiose. The reversal effect of the carbohydrate, which is a potent inhibitor of the lectin's binding to O-linked oligosaccharides of glycoprotein, demonstrate involvement of the lectins interaction with O-glycans of glycoproteins in the consumption of guinea-pig complement.
Hashim
O H
OH
Department of Biochemistry, Faculty of Medicine, University of Malaya, Kuala Lumpur.
Gendeh
G S
GS
Cheong
C N
CN
Jaafar
M I
MI
D000818:Animals; D015941:Complement Hemolytic Activity Assay; D003165:Complement System Proteins / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D006168:Guinea Pigs; D007369:Interferon Inducers / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D037121:Plant Lectins; D012756:Sheep
D007369:Interferon Inducers; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin; D003165:Complement System Proteins