[The relationship between vaccinia virus DNA replication and intermediate filaments].
Various DNA components which were extracted with gentle cell fractionation from the HeLa cells after 4 h vaccinia virus infection were detected by dot hybridization technique. The virus DNA mainly exist in intermediate filament-lamina-nuclear matrix complex. With DGD embedment free technique and electron microscopic autoradiography, the newly synthesized virus DNA is found to be associated with intermediate filaments. The results of southwestern hybridization demonstrate that vaccinia virus DNA has specific affinity to intermediate filaments and some nuclear matrix proteins.
Yu
W D
WD
Department of Biology, Peking University, Beijing.
Yu
Q
Q
Ding
M X
MX
Zhai
Z H
ZH
D015139:Blotting, Southern; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism*; D014616:Vaccinia virus / Q000235:genetics*
D004279:DNA, Viral