title: Immature CD45RA(low)RO(low) T cells in the human cord blood. I. Antecedents of CD45RA+ unprimed T cells.
abstract: A subset of resting T cells expressing low levels CD45RA and CD45RO molecules (< 1 x 10(3)/cell; CD45RA(low)CD45RO(low)) but high levels of CD45RB and CD38 (5 to 25 x 10(3)/cell) were identified in human cord blood. When these CD45RA(low)RO(low) cells were isolated, they failed to survive in culture (< 10% viability at day 3) unless they were co-cultured on fibroblast monolayers. During the co-culture with fibroblasts, these lymphocytes acquired high levels of cytoplasmic and then membrane CD45RA by day 3 without signs of activation. When stimulated with PHA in the absence of fibroblasts the CD45RA(low)RO(low) cells required monocytes or IL-1 to respond; they rapidly perished if neither were present. On optimal mitogenic stimulation for 48 h in the presence of monocytes, > 90% of CD45RA(low)RO(low) T cells showed only transient CD45RA expression and rapidly acquired CD45RO reactivity. After activation for 48 h the stimulated CD45RA(low)RO(low) subset synthesized high levels of IL-2, comparable to mature peripheral T cells. No IL-4 was detected in these stimulated cultures of cord blood T cells. These data taken together suggest that CD45RA(low)RO(low) T cells in the cord blood are the relatively immature antecedents of CD45RA+RO T cells that require stromal factors for survival in a resting state. The same cells need monocytes or IL-1 for their activation to develop after a short CD45RA+ stage into activated CD45RO+RA(low) T cells with potent IL-2 biosynthetic capacity. An additional study of these cells is warranted to confirm that they are in fact the recent emigrants from the thymus, as suggested by similar observations in animal models.
authors.affiliation: Department of Clinical Immunology, Royal Free Hospital and School of Medicine, London, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bofill
authors.forename: A N
authors.initials: AN
authors.lastname: Akbar
authors.forename: M
authors.initials: M
authors.lastname: Salmon
authors.forename: M
authors.initials: M
authors.lastname: Robinson
authors.forename: G
authors.initials: G
authors.lastname: Burford
authors.forename: G
authors.initials: G
authors.lastname: Janossy
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D003584:Cytological Techniques; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens / Q000097:blood*; D008213:Lymphocyte Activation; D010641:Phenotype; D011247:Pregnancy; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
chemical_list: D007376:Interleukin-2; D015847:Interleukin-4; D017493:Leukocyte Common Antigens