title: Microinjection of HEp-2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV-inactivated virus.
abstract: Coxsackie B1 virus induces increased susceptibility to invasion by Shigella flexneri when HEp-2 cells are inoculated with the complete virus. When RNA from the same virus was microinjected into cells, virus RNA was synthesized and new virus particles were formed, but the transfected RNA had no effect on bacterial invasiveness. However, when the cells were prestimulated with UV-inactivated virus, the microinjected RNA induced an additional enhancement of bacterial invasiveness. Microinjected whole virus particles did not replicate and did not induce any change in bacterial invasiveness. The results indicate that an initial event in virus multiplication is necessary to achieve an effect of transfected viral RNA on invasion of S. flexneri.
authors.affiliation: Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Norway.
authors.forename: K R
authors.initials: KR
authors.lastname: Modalsli
authors.forename: S O
authors.initials: SO
authors.lastname: Mikalsen
authors.forename: G
authors.initials: G
authors.lastname: Bukholm
authors.forename: M
authors.initials: M
authors.lastname: Degré
mesh_terms: D003600:Cytosol / Q000382:microbiology; D029822:Enterovirus B, Human / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects*; D006801:Humans; D008845:Microinjections; D012367:RNA, Viral / Q000008:administration & dosage / Q000494:pharmacology*; D012763:Shigella flexneri / Q000502:physiology*; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000528:radiation effects
chemical_list: D012367:RNA, Viral