Isolation and identification of cecropin antibacterial peptides from the extracellular matrix of the insect integument.
A method for the screening and isolation of antibacterial peptides from the extracellular insect cuticle using ultrafiltration and reverse-phase high-performance liquid chromatography is presented. Using this method we were able to conveniently isolate cuticular antibacterial peptides belonging to the cecropin family in 1 day. The procedure should facilitate the rapid screening of antibacterial peptides or other bioactive peptides from extracellular matrices.
Lee
W J
WJ
Unité d'Ecologie des Systèmes Vectoriels, Institut Pasteur, Paris, France.
Brey
P T
PT
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification*; D012831:Bombyx / Q000737:chemistry*; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000737:chemistry*; D007301:Insect Hormones / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000494:pharmacology; D014462:Ultrafiltration / Q000379:methods
D000890:Anti-Infective Agents; D007301:Insect Hormones; D010446:Peptide Fragments