Purification and characterization of two putative HLA class II associated proteins: PHAPI and PHAPII.
In addition to their well defined role in presentation of processed antigen on the cell surface, class II molecules are able to transduce signals into the cell after binding of ligands. The cytoplasmic regions of class II molecules might function as docking sites for as yet unidentified proteins that are components of this signalling pathway. Here we report on two putative HLA class II associated proteins (PHAPI and PHAPII) which have been purified from the cytosolic fraction of the human lymphoblastoid B-cell line H2LCL using an affinity matrix composed of the synthetic biotinylated cytoplasmic region of the DR2 alpha chain immobilized on avidin agarose. The sequence obtained for PHAPI revealed a novel primary structure with a leucine/isoleucine rich N-terminal region. Protein data and the cDNA sequence obtained for PHAPII agree with the cDNA sequence of SET that has been described recently. Both PHAPI and PHAPII have an extended highly acidic C-terminal region. Based on their primary structure we speculate that PHAPI and PHAPII are involved in the generation of intracellular signalling events that lead to regulation of transcriptional activity after binding of a ligand to HLA class II molecules.
Vaesen
M
M
Max-Planck-Institute for Experimental Mecdicine, Department of Immunochemistry, Göttingen, Germany.
Barnikol-Watanabe
S
S
Götz
H
H
Awni
L A
LA
Cole
T
T
Zimmermann
B
B
Kratzin
H D
HD
Hilschmann
N
N
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000737:chemistry; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D056488:Histone Chaperones; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
C087425:ANP32A protein, human; D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D000949:Histocompatibility Antigens Class II; D056488:Histone Chaperones; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; C513473:SET protein, human; D014157:Transcription Factors