title: Cloning and structure of the human corticotrophin releasing factor-binding protein gene (CRHBP)
abstract: The human CRF-binding protein gene has been cloned and mapped to the distal region of chromosome 13 and loci 5q in the mouse and human genomes, respectively. The gene consists of 7 exons and 6 introns. The mature protein has 10 cysteines and 5 tandem disulfide bridges 4 of which are contained within exons 3, 5, 6, and 7. One bridge is shared by exons 3 and 4. The signal peptide and the first 3 amino acids of the mature protein were coded for by an extreme 5' exon. Primer extension analyses revealed the transcriptional initiation site to be located 32 bp downstream from a consensus TATA box. The promoter sequence contained a number of putative promoter elements including an AP-1 site, three ER-half sites, the immunoglobulin enhancer elements NF-kappa B and INF-1, and the liver-specific enhancers LFA1 and LFB1.
authors.affiliation: Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, San Diego, California 92037.
authors.forename: D P
authors.initials: DP
authors.lastname: Behan
authors.forename: E
authors.initials: E
authors.lastname: Potter
authors.forename: K A
authors.initials: KA
authors.lastname: Lewis
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: N
authors.initials: N
authors.lastname: Copeland
authors.forename: P J
authors.initials: PJ
authors.lastname: Lowry
authors.forename: W W
authors.initials: WW
authors.lastname: Vale
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
chemical_list: D002352:Carrier Proteins; C067133:corticotropin releasing factor-binding protein; D004247:DNA; D003346:Corticotropin-Releasing Hormone