Role of MAPs and motors in the bundling and shimmering of native microtubules from insect ovarioles.
Bundles of native microtubules isolated from the ovarioles of hemipteran insects are seen to shimmer when observed using dark-field microscopy. This novel form of microtubule motility becomes even more obvious when the isolated bundles are detergent-extracted and reactivated. We have studied the nucleotide-specificity and the drug-sensitivity of microtubule shimmering in order to obtain information regarding the nature of the motor protein responsible, and to compare its properties with those of previously characterised microtubule motors. The involvement of structural MAPs in the shimmering and in maintenance of microtubule bundles in this system has also been investigated.
Hunt
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C
University of Exeter, Department of Biological Sciences, Washington Singer Laboratories, United Kingdom.
Stebbings
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H
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D002458:Cell Fractionation; D005260:Female; D006430:Hemiptera / Q000648:ultrastructure*; D008853:Microscopy / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008870:Microtubules / Q000502:physiology*; D009068:Movement; D017830:Octoxynol / Q000494:pharmacology; D010053:Ovary / Q000648:ultrastructure*
D008869:Microtubule-Associated Proteins; D000255:Adenosine Triphosphate; D017830:Octoxynol