Flow cytometrical considerations. The results of three different ways of nuclear preparation and two ways of calculating upon DNA index, number of populations and S-phase rate in endometrial tissue.
Histopathologically normal endometrial tissues were used to compare the preparation methods of Tribukait, Thornthwaite and Vindelöv. Three hundred and forty-one preparations were analyzed giving as a result significant differences regarding the DNA-indices, number of DNA-populations and percentage of cells in S-phase fraction. We also compared a manual way of calculation to a semi-automatic method (RFIT). No difference was found between the manual way and RFIT provided that the same internal standard was used. However, the two different internal standards gave different results within the same method of preparation.
Lindahl
B
B
Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden.
Willen
R
R
D002467:Cell Nucleus / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis*; D004717:Endometrium / Q000648:ultrastructure*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D012189:Retrospective Studies; D016196:S Phase / Q000502:physiology*; D013048:Specimen Handling / Q000379:methods*
D004247:DNA