Factors influencing detection of quantitative cytomegalovirus antigenemia.
Of 20 blood specimens testing positive for cytomegalovirus antigen after immediate processing, 19 (95%) remained positive when kept at room temperature for 24 h before processing. Quantitative antigenemia decreased by an average of 44% after storage. Compared with acetone fixation, formaldehyde fixation showed improved readability, fewer artifacts, and a higher degree of sensitivity.
Boeckh
M
M
Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Woogerd
P M
PM
Stevens-Ayers
T
T
Ray
C G
CG
Bowden
R A
RA
D000096:Acetone; D000956:Antigens, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D012680:Sensitivity and Specificity; D014766:Viremia / Q000175:diagnosis* / Q000382:microbiology; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D000956:Antigens, Viral; D005404:Fixatives; D000096:Acetone; D005557:Formaldehyde