title: Crystallization and preliminary X-ray analysis of an anti-staphylococcal nuclease-staphylococcal nuclease complex and of a second anti-staphylococcal nuclease antibody.
abstract: The Fab fragments of several monoclonal antibodies that bind Staphylococcal nuclease have been screened for crystallization conditions. Two of these, N10 and N25, have been crystallized in forms suitable for X-ray structural analysis. The anti-Staphylococcal nuclease antibody complex N10 Fab-nuclease crystallizes with symmetry consistent with space group C2 and cell parameters of a = 234.7 A; b = 43.5 A; c = 74.4 A; beta = 106.4 degrees. A second anti-Staphylococcal nuclease antibody, N25, although crystallized starting with the Fab-nuclease complex, apparently crystallizes as uncomplexed N25 Fab with symmetry consistent with space group P3(1)21 (or its enantiomorph P3(2)21) and cell parameters of a = b = 80.9 A; c = 138.4 A.
authors.affiliation: Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543-4000.
authors.forename: C Y
authors.initials: CY
authors.lastname: Chang
authors.forename: P B
authors.initials: PB
authors.lastname: Whitaker
authors.forename: L
authors.initials: L
authors.lastname: Tabernero
authors.forename: H
authors.initials: H
authors.lastname: Einspahr
authors.forename: L
authors.initials: L
authors.lastname: Workman
authors.forename: D C
authors.initials: DC
authors.lastname: Benjamin
authors.forename: S
authors.initials: S
authors.lastname: Sheriff
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D003460:Crystallization; D018360:Crystallography, X-Ray; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000302:isolation & purification; D008836:Micrococcal Nuclease / Q000737:chemistry* / Q000302:isolation & purification; D013210:Staphylococcus / Q000201:enzymology / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D008836:Micrococcal Nuclease