Site-specific DNA cleavage by Cu(II) complexes of podophyllotoxin derivatives.
Site-specific DNA cleavage in the presence of Cu(II) complexes of podophyllotoxin derivatives was investigated with a modified Sanger sequencing method. Cu(II) complexes of 4'-demethylepipodophyllotoxin (DEPD) and syringic acid (SA) cleaved M13mp18 single-strand DNA site-specifically at both cytosine (C) and guanine (G) positions in the GC rich regions and C position, respectively, at pH 7.8. The apparent binding constants of calf thymus DNA-Cu(II) complexes estimated by the differential UV-absorption spectra revealed that both Cu(II)-VP-16 and -DEPD complexes bind to DNA more strongly than does the Cu(II)-SA complex.
Yamashita
A
A
Department of Analytical Chemistry, Kyoto Pharmaceutical University, Japan.
Tawa
R
R
Imakura
Y
Y
Lee
K H
KH
Sakurai
H
H
D001483:Base Sequence; D003300:Copper / Q000494:pharmacology*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects / Q000235:genetics; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011034:Podophyllotoxin / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
D004277:DNA, Single-Stranded; D005707:Gallic Acid; D003300:Copper; C001945:syringic acid; D004259:DNA-Directed DNA Polymerase; D011034:Podophyllotoxin