Significant stabilization of the phosphatidylcholine bilayer structure by incorporation of small amounts of cardiolipin.
The effects of the negatively charged phospholipid cardiolipin on the structural properties of egg-yolk phosphatidylcholine (EyPC) liposomal membranes were studied by monitoring the water permeability of the liposomes caused by osmotic shrinkage in hypertonic glucose solution. Incorporation of small amounts of bovine heart cardiolipin (BhCL) into the EyPC membranes caused a significant decrease in their water permeability associated with stabilization of the membrane structure. Much evidence obtained by attenuated total reflection IR spectroscopy suggested that incorporation of BhCL into the EyPC membranes causes a cooperative conformational change in the EyPC polar head groups, but does not alter the fluidity of the bilayer structure in the fluid liquid crystalline state. Incorporation of small amounts of BhCL stabilized the intermolecular hydrogen-bonded network including water molecules of the hydration layers at the bilayer surface that are important for the stable bilayer configuration of the EyPC molecules. The antisymmetric PO2- frequencies of the EyPC membrane with incorporated BhCL suggested that the BhCL content of 50 mol% induced a change in the phase behaviors of mixed BhCL/EyPC membranes.
Shibata
A
A
Faculty of Pharmaceutical Sciences, University of Tokushima Shomachi-1, Japan.
Ikawa
K
K
Shimooka
T
T
Terada
H
H
D000818:Animals; D002308:Cardiolipins / Q000737:chemistry*; D002417:Cattle; D004355:Drug Stability; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D010539:Permeability; D010710:Phosphates / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014867:Water
D002308:Cardiolipins; D008051:Lipid Bilayers; D008081:Liposomes; D010710:Phosphates; D010713:Phosphatidylcholines; D014867:Water