title: The effect of in vitro procedures on cyclic AMP accumulation in human leucocytes.
abstract: The aim of this study was to investigate the effect of various methodological procedures or protocols on cyclic AMP formation in human leucocytes. The data showed that: (1) ATP content and lactate production was unaffected by hypotonic lysis during leucocyte isolation; (2) there was a linear relation between cell number/sample and the production of cyclic adenosine 3':5'-monophosphate (cAMP); (3) the interindividual variation markedly affected cAMP production during long observation periods (years), whereas day-to-day variation within a week was less important; (4) whole blood could be stored for up to 4 h (at 4 degrees C or 23 degrees C) without affecting cAMP accumulation; (5) isolated MNL could be stored for up to 2 h (at 4 degrees C) without affecting cAMP accumulation; and finally that (6) choice and concentration of phosphodiesterase inhibitors markedly influenced the basal and isoprenaline-induced cAMP accumulation. Evidence is provided that the rate of cAMP accumulation in response to isoprenaline of cells incubated in the presence of 2.5 mM 3-isobutyl-1-methyl-xanthine (IBMX) is reflecting the rate of cellular cAMP production.
authors.affiliation: Department of Pharmacology, Institute of Medical Biology, Odense University, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Johansen
authors.forename: U G
authors.initials: UG
authors.lastname: Friis
authors.forename: A K
authors.initials: AK
authors.lastname: Rasmussen
authors.forename: N
authors.initials: N
authors.lastname: Foged
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D002470:Cell Survival / Q000502:physiology; D000242:Cyclic AMP / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000097:blood; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D011786:Quality Control; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D007773:Lactates; D011943:Receptors, Adrenergic, beta; D000255:Adenosine Triphosphate; D000242:Cyclic AMP