title: p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair.
abstract: The human BTF2 (TFIIH) transcription factor is a multisubunit protein involved in transcription initiation by RNA polymerase II (B) as well as in DNA repair. In addition to the previously characterized p62 and p89/ERCC3 subunits, we have cloned two other subunits of BTF2, p44 and p34. The gene encoding p44 appeared to be the human counterpart of SSL1, a gene involved in translation and UV resistance in yeast. Interestingly, the p34 subunit also has homology with a domain of SSL1, suggesting that it corresponds to an as yet unidentified protein involved in DNA repair. Both p44 and p34 possess zinc finger domains that may mediate BTF2 binding to nucleic acids.
authors.affiliation: UPR 6520 (CNRS), Unité 184 (INSERM), Faculté de Médecine, Strasbourg, France.
authors.forename: S
authors.initials: S
authors.lastname: Humbert
authors.forename: H
authors.initials: H
authors.lastname: van Vuuren
authors.forename: Y
authors.initials: Y
authors.lastname: Lutz
authors.forename: J H
authors.initials: JH
authors.lastname: Hoeijmakers
authors.forename: J M
authors.initials: JM
authors.lastname: Egly
authors.forename: V
authors.initials: V
authors.lastname: Moncollin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004260:DNA Repair; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic; D016335:Zinc Fingers
chemical_list: D005656:Fungal Proteins; D046911:Macromolecular Substances; D011994:Recombinant Proteins; C083908:SSL1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C490039:TAF6 protein, S cerevisiae; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH