Solubilization and purification of a high affinity neurotensin receptor from newborn human brain.
High affinity neurotensin receptors were solubilized in an active form from newborn human brain using the non-denaturing detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS). The solubilized receptor was purified in a single step by affinity chromatography. The binding properties of the purified receptor towards [125I-Tyr3]neurotensin are very similar to those of the membrane bound and of the crude CHAPS-solubilized receptor in terms of affinity and specificity. The purified receptor is a single protein chain of molecular weight 100 kDa as shown by gel filtration and by affinity labelling with [125I-Tyr3]neurotensin in the presence of the cross-linking agent disuccinimidyl suberate.
Zsürger
N
N
Institut de Pharmacologie Moléculaire et Cellulaire du CNRS, Valbonne, France.
Mazella
J
J
Vincent
J P
JP
D000595:Amino Acid Sequence; D001923:Brain Chemistry / Q000502:physiology*; D002793:Cholic Acids; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007223:Infant; D007457:Iodine Radioisotopes; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009496:Neurotensin / Q000378:metabolism; D018028:Receptors, Neurotensin / Q000302:isolation & purification* / Q000378:metabolism
D002793:Cholic Acids; D007457:Iodine Radioisotopes; D018028:Receptors, Neurotensin; D009496:Neurotensin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate