The mouse creatine kinase paired E-box element confers muscle-specific expression to a heterologous promoter.
E-box elements, with the CANNTG sequence motif, occur in numerous promoters and enhancers. We evaluated the tissue-specific expression properties of the paired murine E-box element from the mouse muscle creatine kinase (MCK) enhancer in a minimal heterologous promoter construct. A 46-bp fragment containing the paired E-box element in its wild-type (wt) configuration conferred high levels of muscle-specific expression in transfected embryonic chicken cell cultures. The expression from this paired E-box element was similar to that of the simian virus 40 (SV40) promoter/enhancer, but a 21-bp fragment containing a single E-box was inactive. We conclude that the paired E-box element from the MCK enhancer is sufficient for high levels of muscle-specific expression when placed upstream from a non-muscle TATA element.
Martin
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KA
Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106.
Walsh
K
K
Mader
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SL
D000199:Actins / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000096:biosynthesis / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D016762:Genes, fos / Q000235:genetics; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*
D000199:Actins; D003402:Creatine Kinase