Overexpression of the Raf-1 proto-oncogene in human myeloid leukemia.
The Raf-1 protein, a cytoplasmic serine/threonine kinase, plays an important role in signal transduction pathways. In order to examine the role of Raf-1 in human myeloid leukemia, we determined raf-1 mRNA expression by Northern blot analysis in blast cell samples from 27 acute myeloid leukemia (AML) cases and peripheral blood mononuclear cells from six healthy donors. A normal raf-1 transcript size was detected in all cases investigated. However, overexpression of raf-1 mRNA was found in 2 of 27 AML cases, both of which were erythroleukemias (AML, FAB M6).
Schmidt
C A
CA
Abteilung für Innere Medizin und Poliklinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, F.R.G.
Oettle
H
H
Ludwig
W D
WD
Serke
S
S
Pawlaczyk-Peter
B
B
Wilborn
F
F
Binder
L T
LT
Huhn
D
D
Siegert
W
W
D000208:Acute Disease; D001483:Base Sequence; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000097:blood / Q000235:genetics; D007951:Leukemia, Myeloid / Q000097:blood / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D019908:Proto-Oncogene Proteins c-raf; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood; D012016:Reference Values
D017931:DNA Primers; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf