Synthesis and binding of new polyfluorinated aryl azides to alpha-chymotrypsin. New reagents for photoaffinity labeling.
The preparation of several new, water-soluble, polyfluorinated aryl azide reagents for use in photoaffinity labeling studies is described. The ability of some of these reagents and their nonfluorinated controls to noncovalently bind to alpha-chrymotrypsin is reported. It is found that polyfluorination does not interfere with ligand binding.
Soundararajan
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Department of Chemistry, Ohio State University, Columbus 43210.
Liu
S H
SH
Soundararajan
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S
Platz
M S
MS
D000345:Affinity Labels / Q000138:chemical synthesis* / Q000737:chemistry; D001386:Azides / Q000737:chemistry*; D001667:Binding, Competitive; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000494:pharmacology; D007700:Kinetics; D011485:Protein Binding; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry
D000345:Affinity Labels; D001386:Azides; D007211:Indoles; D014443:Tyrosine; D002918:Chymotrypsin