title: Detection of Epstein-Barr virus in a case of undifferentiated nasopharyngeal carcinoma by in situ hybridization with digoxigenin-labelled PCR-generated probes.
abstract: A case of nasopharyngeal carcinoma is presented. Epstein-Barr viral genome was identified in the neoplastic cells by in situ hybridization with digoxigenin-labelled polymerase chain reaction-generated probes. We report the development of this technique in paraffin-embedded sections and propose that such identification may prove valuable for the diagnosis of this tumour in routine material.
authors.affiliation: Department of Pathology, University Hospital of Liège, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Delvenne
authors.forename: B
authors.initials: B
authors.lastname: Kaschten
authors.forename: J M
authors.initials: JM
authors.lastname: Deneufbourg
authors.forename: L
authors.initials: L
authors.lastname: Demanez
authors.forename: A
authors.initials: A
authors.lastname: Stevenaert
authors.forename: M
authors.initials: M
authors.lastname: Reznik
authors.forename: J
authors.initials: J
authors.lastname: Boniver
mesh_terms: D000328:Adult; D001483:Base Sequence; D002277:Carcinoma / Q000382:microbiology* / Q000473:pathology; D004076:Digoxigenin; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D008279:Magnetic Resonance Imaging; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction
chemical_list: D015335:Molecular Probes; D004076:Digoxigenin