Gene regulation of trkB and trkC in the chick retina by light/darkness exposure.
The trk gene family members; the neurotrophic receptors for neurotrophins, are implicated in the survival and the differentiation of neurons. The roles of these protooncogenes have been argued in the pathological conditions and in the specific developmental stage when the programmed cell death occurs to neurons. Here we studied a physiological role of the trk family members in the retina through observations of their gene regulation by light/darkness exposure. Northern blot analysis and immunohistochemistry demonstrate that trkB and trkC are up-regulated by light exposure and down-regulated by darkness in the rod/cone layer, the outer nuclear layer, and the ganglion cell layer. This physiological regulation suggests that these trk family members play a protective role from the damaging effect of light exposure in the retinal neurons.
Okazawa
H
H
Department of Neurology, Faculty of Medicine, University of Tokyo, Japan.
Kamei
M
M
Imafuku
I
I
Kanazawa
I
I
D000818:Animals; D015152:Blotting, Northern; D002645:Chickens; D015342:DNA Probes; D018076:DNA, Complementary; D003624:Darkness; D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000528:radiation effects; D007150:Immunohistochemistry; D008027:Light; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020801:Receptor, Ciliary Neurotrophic Factor; D020812:Receptor, trkC; D017978:Receptors, Growth Factor / Q000235:genetics*; D012160:Retina / Q000378:metabolism*; D015854:Up-Regulation
D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D017978:Receptors, Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020812:Receptor, trkC