Application of electrospray and fast atom bombardment mass spectrometry to the identification of post-translational and other chemical modifications of proteins and peptides.
Mass spectrometry is a very powerful tool in the identification of chemical modifications of proteins and peptides. Often these modifications cannot be determined by conventional techniques. This report describes the combined use of electrospray ionization mass spectrometry and fast atom bombardment mass spectrometry to complete the primary structure of proteins and peptides. Examples illustrate how mass spectrometry is used to locate sites of phosphorylation, methylation and acetylation, and identify blocking groups and unexpected side reactions such as deamidation or alkylation.
Kouach
M
M
URA 409 CNRS, Université de Lille II, France.
Belaïche
D
D
Jaquinod
M
M
Couppez
M
M
Kmiecik
D
D
Ricart
G
G
Van Dorsselaer
A
A
Sautière
P
P
Briand
G
G
D000107:Acetylation; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003500:Cyclization; D004563:Electrochemistry; D004912:Erythrocytes / Q000737:chemistry; D006657:Histones / Q000737:chemistry; D008297:Male; D008745:Methylation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D010766:Phosphorylation; D011479:Protamines / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry*; D012756:Sheep; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013950:Thymus Gland / Q000737:chemistry; D014982:Xenopus laevis
D000596:Amino Acids; D006657:Histones; D010455:Peptides; D011479:Protamines; D011506:Proteins