Biosynthetic lipid-tagging of antibodies.
Chemical conjugation of fatty acids to antibodies generates lipid-modified molecules which have found use in the targeting of liposome-mediated drug delivery and in liposome-based immunoassays. Alternatively, bacterial expression of antibodies as single-chain FV fragments fused to lipoprotein signal peptide and N-terminal sequence leads to in vivo enzymatic addition of a single glycerolipid group at the N-terminus of the molecule. This lipid-modification converts the antibody from a soluble protein into a functional membrane-bound molecule. These biosynthetically lipid-tagged antibodies may prove useful for immobilization of antibodies to membranes in various biotechnological applications.
Keinänen
K
K
VTT Biotechnology and Food Research, Espoo, Finland.
Laukkanen
M L
ML
D000906:Antibodies; D001426:Bacterial Proteins; D004337:Drug Carriers; D005818:Genetic Engineering; D007118:Immunoassay; D008055:Lipids; D008074:Lipoproteins; D008081:Liposomes
D000906:Antibodies; D001426:Bacterial Proteins; D004337:Drug Carriers; D008055:Lipids; D008074:Lipoproteins; D008081:Liposomes