Antibody-dependent cellular cytotoxicity and neutralization of human immunodeficiency virus type 1 by high affinity cross-linking of gp41 to human macrophage Fc IgG receptor using bispecific antibody.
Human monocytes/macrophages, which express Fc receptors for IgG are involved in human immunodeficiency virus type 1 (HIV-1) infection and pathogenesis. These receptors are known to mediate numerous immunological functions including cell-mediated killing and possibly targeting of HIV to the lysophagosome monocyte-derived macrophage (MDM) entry route for virus neutralization. To study both activities in HIV-1 infection, MDM Fc gamma RI was specifically selected using bispecific antibody (Bs-Ab) containing whole human monoclonal antibody against gp41 and the Fab' fragment of murine anti-Fc gamma RI 22.2 antibody. Bs-Ab was found to mediate potent antibody-dependent cellular cytotoxicity and virus neutralization.
Mabondzo
A
A
Laboratoire de Neuropathologie expérimentale et Neurovirologie, CRSSA, DSV/DPTE, Commissariat à l'Energie Atomique, Fontenay-aux-Roses, France.
Boussin
F
F
Raoul
H
H
Le Naour
R
R
Gras
G
G
Vaslin
B
B
Bartholeyns
J
J
Romet-Lemonne
J L
JL
Dormont
D
D
D018033:Antibodies, Bispecific; D000920:Antibody-Dependent Cell Cytotoxicity; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015658:HIV Infections / Q000628:therapy*; D015497:HIV-1 / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D009500:Neutralization Tests; D011533:Proviruses / Q000737:chemistry; D017452:Receptors, IgG / Q000276:immunology*
D018033:Antibodies, Bispecific; D004279:DNA, Viral; D015700:HIV Envelope Protein gp41; D017452:Receptors, IgG