Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells.
Ecotropic recombinant virus (ERV), a relatively new class of murine retrovirus endogenous to mice, is expressed at significant levels by most murine myeloma and hybridoma cells examined. The routine XC, S+L-, mink cell focus-inducing (MCF), and reverse transcriptase (RT) tests are not suitable to detect and quantify the levels of ERV. A serological focus assay, based on specific anti-murine leukemia virus (MuLV) viral envelope (env) antibodies, is required to detect ERV. A more sensitive format of this serological focus assay includes co-cultivation of test article cells with the indicator (Mus dunni) cells. ERV isolated from murine hybridoma cells show a unique pattern of cross-reactivity with anti-MuLV env antibodies and this pattern is clearly distinct from that of ecotropic and xenotropic retroviruses.
Deo
Y
Y
Centocor Inc., Malvern, PA.
Ghebremariam
H
H
Cloyd
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D017988:Gammaretrovirus / Q000276:immunology / Q000302:isolation & purification*; D006825:Hybridomas / Q000166:cytology / Q000382:microbiology*; D009052:Leukemia Virus, Murine / Q000276:immunology; D051379:Mice; D008907:Mink; D009101:Multiple Myeloma / Q000382:microbiology*; D009115:Muridae; D014407:Tumor Cells, Cultured / Q000382:microbiology*; D010948:Viral Plaque Assay
D000914:Antibodies, Viral