Serum stimulation of UDP-glucose dehydrogenase activity in Swiss 3T3 fibroblasts.
A new HPLC assay was developed to measure UDP-glucose dehydrogenase (UDP-GDH) activity in crude homogenates of 3T3 fibroblasts. UDP-GDH activity is directly related to the proliferative activity of the cell culture: enzyme activity is highest in log phase cells and decreases as the culture approaches quiescence. Serum stimulation of quiescent 3T3 fibroblasts results in an increase in UDP-GDH activity that has two components that are differentially affected by inhibitors of protein synthesis. Following serum stimulation, changes in cellular UDP-glucuronic acid concentrations mirror changes in UDP-GDH activity. UDP-xylose is a potent inhibitor of UDP-GDH but inhibitory concentrations of UDP-xylose could not be detected in cell extracts.
Grubb
M F
MF
Laboratory of Medicinal Chemistry, National Cancer Institute, NIH, Bethesda, MD 20892.
Kasofsky
J
J
Strong
J
J
Anderson
L W
LW
Cysyk
R L
RL
D016475:3T3 Cells; D000818:Animals; D001790:Blood Physiological Phenomena; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D051379:Mice; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000378:metabolism*; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism
D003470:Culture Media; D014535:Uridine Diphosphate Glucuronic Acid; D014533:Uridine Diphosphate Glucose Dehydrogenase