Pre-messenger RNA splicing of transcripts synthesized from human small nuclear RNA gene promoters.
In order to explore the coupling of transcription with splicing in mammalian cells we have prepared hybrid genes in which either the human U2 promoter, recognized by RNA polymerase II, or the human U6 promoter, recognized by RNA polymerase III, was fused to an intron-containing gene segment. Neither human small nuclear RNA gene contains an intron although U6 genes from some species of yeast contain a short intervening sequence. Following transfection of human cells and analysis of specific RNAs by primer extension we found that the chimeric U2 promoter-derived transcript was efficiently spliced but the RNA polymerase III transcript driven by the U6 promoter remained unspliced. Hence, the splicing apparatus differentiates between transcripts produced from two closely related promoters that are distinguished by RNA polymerase selectivity.
White
R A
RA
Department of Biochemistry and Biophysics, Texas A & M University, College Station 77843-2128.
Kunkel
G R
GR
D001483:Base Sequence; D002478:Cells, Cultured; D004274:DNA, Recombinant; D006801:Humans; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012319:RNA Polymerase II / Q000378:metabolism; D012320:RNA Polymerase III / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics
D004274:DNA, Recombinant; D012322:RNA Precursors; D012342:RNA, Small Nuclear; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D012319:RNA Polymerase II; D012320:RNA Polymerase III