Metabolism of 1-(4-hydroxy-3-methoxyphenyl)-2-propanone, a smoke flavour ketone, in rat.
1. Metabolites of 1-(4-hydroxy-3-methoxyphenyl)-2-propanone (HMP-one), a smoke flavour compound, were isolated from rat urine using hydrolysis, ether extraction, t.l.c. and g.l.c. 2. Three metabolites were identified by mass spectrometry and independent synthesis, namely: 1-(3, 4-dihydroxyphenyl)-2-propanone (Met I), 1-(3, 4-dihydroxyphenyl)-2-propanol (Met II), and 1-(4-hydroxy-3-methoxyphenyl)-2-propanol (Met III). 3. A g.l.c. method for the quantitative determination of the parent compound and metabolites in urine was devised. Unchanged HMP-one accounted for about 74% dose, with Met I 11%, Met II 5%, and Met III 9%. All compounds were excreted both as sulphate and glucuronide conjugates.
Jodynis-Liebert
J
J
Department of Toxicology, Academy of Medicine, Poznań, Poland.
D000096:Acetone / Q000031:analogs & derivatives* / Q000652:urine; D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005421:Flavoring Agents; D005965:Glucuronates / Q000652:urine; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000652:urine; D006868:Hydrolysis; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000652:urine
D005421:Flavoring Agents; D005965:Glucuronates; D013431:Sulfates; C083441:1-(4-hydroxy-3-methoxyphenyl)-2-propanone; D000096:Acetone; D006139:Guaiacol