title: Clinical and histological evaluation of ligature-induced periodontitis in the domestic ferret.
abstract: The aim of this study was to evaluate the clinical and histological characteristics of ligature induced periodontitis in 12 domestic ferrets. On day 0, silk ligatures were placed at the gingival margin level of selected experimental teeth. The contralateral teeth served as control teeth. On day 14, 4 animals and on day 28, 8 animals were sacrificed. Clinical measurements including gingival index (GI), probing pocket depth (PPD) and probing attachment level (PAL) were taken in 5 animals on days 0, 14 and 28. Histological sections were prepared for light microscopy. The histometric measurements performed were (1) the distance between the cemento-enamel junction (CEJ) and the alveolar bone crest and (2) the distance between CEJ and connective tissue attachment level. The number of sections with root resorption areas was also observed. Cell counts were taken in 4 different areas. Clinical results showed, on days 14 and 28, a significant increase of the mean values of PPD and PAL at the experimental teeth when compared to the control teeth. The histometric results showed a significant loss of connective tissue attachment and bone loss in the experimental teeth on day 14. On day 28, the results were similar, although more sections with root resorption areas were observed in the experimental teeth. The predominant inflammatory cell in all the 4 experimental areas analyzed was polymorphonuclears (PMN), and they were mainly increased in areas closely related to the junctional epithelium. The results indicate that the domestic ferret may be used as an experimental animal in the ligature-induced periodontitis model.
authors.affiliation: Department of Periodontology, Centre for Oral Health Sciences, Lund University, Malmö, Sweden.
authors.forename: R G
authors.initials: RG
authors.lastname: Fischer
authors.forename: B
authors.initials: B
authors.lastname: Klinge
mesh_terms: D016301:Alveolar Bone Loss / Q000473:pathology; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D002452:Cell Count; D003238:Connective Tissue / Q000473:pathology; D003739:Dental Cementum / Q000473:pathology; D003743:Dental Enamel / Q000473:pathology; D003804:Dentin / Q000473:pathology; D004195:Disease Models, Animal; D004846:Epithelial Attachment / Q000473:pathology; D005260:Female; D005289:Ferrets; D005347:Fibroblasts / Q000473:pathology; D005884:Gingival Hemorrhage / Q000473:pathology; D007958:Leukocyte Count; D008026:Ligation; D008297:Male; D009504:Neutrophils / Q000473:pathology; D017622:Periodontal Attachment Loss / Q000473:pathology; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000473:pathology; D010518:Periodontitis / Q000209:etiology / Q000473:pathology*; D012391:Root Resorption / Q000473:pathology