Dynamin binds to SH3 domains of phospholipase C gamma and GRB-2.
Src homology 3 (SH3) domains are found in a variety of proteins that are involved in signal transduction or represent components of the cytoskeleton. These domains are thought to serve as modules that mediate specific protein-protein interactions that include proline-rich sequences on the target protein. We have identified proteins of 110, 80, 65, and 43 kDa in human embryonic fibroblasts that bind specifically to the SH3 domain of phospholipase C gamma, a primary substrate of receptor tyrosine kinases, and characterized the 110-kDa band as the microtubule-activated GTPase dynamin. In addition, dynamin binds the son of sevenless adaptor protein GRB-2 with even higher affinity. This interaction does not require the dynamin GTPase function and involves a proline-rich target sequence between residues 812 and 820 of dynamin.
Seedorf
K
K
Department of Molecular Biology, Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Kostka
G
G
Lammers
R
R
Bashkin
P
P
Daly
R
R
Burgess
W H
WH
van der Bliek
A M
AM
Schlessinger
J
J
Ullrich
A
A
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D034281:Dynamins; D051380:GRB2 Adaptor Protein; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D016391:Genes, src; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008870:Microtubules / Q000201:enzymology*; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D011506:Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D010738:Type C Phospholipases; D020558:GTP Phosphohydrolases; D034281:Dynamins