title: An improved method for rapid fixation and embedding of pteridophyte plant materials.
abstract: A rapid method for fixation and embedding of plant materials, especially pteridophytes, is suggested. Addition of tannic acid following osmication improved the visualization of membranes. Staining en bloc with uranyl acetate between osmication and tannic acid is suggested for tissues infected with fungi and bacteria.
authors.affiliation: Electron Microscopy Section, National Botanical Research Institute, Lucknow, India.
authors.forename: S
authors.initials: S
authors.lastname: Devi
authors.forename: V K
authors.initials: VK
authors.lastname: Lall
authors.forename: P
authors.initials: P
authors.lastname: Garg
authors.forename: A
authors.initials: A
authors.lastname: Saxena
authors.forename: M
authors.initials: M
authors.lastname: Tiwari
mesh_terms: D002101:Cacodylic Acid; D005404:Fixatives; D005976:Glutaral; D009942:Organometallic Compounds; D009993:Osmium Tetroxide; D059828:Plant Cells; D010935:Plant Diseases; D016610:Tissue Embedding; D016707:Tissue Fixation
chemical_list: D005404:Fixatives; D009942:Organometallic Compounds; C005460:uranyl acetate; D002101:Cacodylic Acid; D009993:Osmium Tetroxide; D005976:Glutaral