Hybridization-based affinity partitioning of nucleic acids using PEG-coupled oligonucleotides.
Polyethylene glycol (PEG)-coupled oligonucleotides are partitioned in an aqueous two-phase system PEG/dextran. The affinity of the oligonucleotide for the PEG-rich phase increases proportionally to the length of the coupled PEG polymer. After hybridization, the PEG-coupled oligonucleotide is able to force a complementary nucleic acid strand into the PEG-rich phase. This property can be used for the sequence-specific isolation of nucleic acids through hybridization-based affinity partitioning. The dependence of the partition coefficient in this system on various parameters is described. The application of this principle to multistage chromatographic separations is demonstrated.
Jäschke
A
A
Institut für Organische und Bioorganische Chemie, Humboldt-Universität zu Berlin, Germany.
Fürste
J P
JP
Erdmann
V A
VA
Cech
D
D
D001483:Base Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry; D012995:Solubility
D009838:Oligodeoxyribonucleotides; D011092:Polyethylene Glycols