The disbalance of alpha- and beta-globins in anemic Belgrade rat red blood cells.
The Belgrade Laboratory (b/b) rat has an autosomal mutation which in homozygous state induces severe anemia. This study was based on solubilization of total rat globin chains and their separation into alpha- and beta-globins using a 20% SDS polyacrylamide gel. These analyses demonstrated that the disbalance of alpha/beta globins in b/b red blood cells (RBC) is due to decreased level of alpha-globins. Iron-dextran administration corrected the level and globin ratio in b/b RBC thus confirming that the iron deficiency is the primary defect in b/b rats.
Marjanovic
J
J
Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
Savkovic
S
S
Nikcevic
G
G
Glisin
V
V
Ivanovic
Z
Z
Milenkovic
P
P
Popovic
Z
Z
D000740:Anemia / Q000097:blood*; D000818:Animals; D015870:Gene Expression; D005914:Globins / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011922:Rats, Mutant Strains
D012333:RNA, Messenger; D005914:Globins; D007501:Iron