Induction of inflammatory cytokines by Nocardia fractions.
NDCM and NLD fractions of Nocardia opaca cell walls were used for in vitro stimulation of human and porcine peripheral blood mononuclear cells. Inflammatory cytokines IL-1, IL-6, tumour necrosis factor (TNF) alpha and interferon (IFN) gamma were detected at the single-cell level using paraformaldehyde-fixed and saponin-permeabilized mononuclears stained with cytokine-specific antibodies and indirect immunofluorescence method. IL-1, IL-6 and TNF were produced by human monocytes stimulated for 2 h, IFN gamma-positive lymphocytes were detected later. IFN gamma was produced also by activated porcine lymphocytes. Cells expressing apoptotic features were found among blood mononuclears treated with Nocardia fractions.
Trebichavský
I
I
Institute of Microbiology, Department of Immunology and Gnotobiology, Nový Hrádek.
Barot-Ciorbaru
R
R
Charley
B
B
Splíchal
I
I
D000818:Animals; D002463:Cell Membrane Permeability; D017358:Cell Wall Skeleton / Q000276:immunology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007378:Interleukins / Q000096:biosynthesis*; D008070:Lipopolysaccharides; D008934:Mitogens / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D009615:Nocardia / Q000276:immunology* / Q000648:ultrastructure; D009930:Organic Chemicals; D013552:Swine; D013556:Swine, Miniature; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D017358:Cell Wall Skeleton; D007378:Interleukins; D008070:Lipopolysaccharides; D008934:Mitogens; C101327:Nocardia delipidated cell mitogen; D009930:Organic Chemicals; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma