title: Trehalose metabolism during sporulation in Saccharomyces cerevisiae.
abstract: S. cerevisiae has at least two enzymes for trehalose synthesis. In this paper we report some characteristics of the ADPG-dependent trehalose-6-phosphate synthase. As opposed to the UDPG-dependent enzyme, the ADPG-dependent activity did not respond to a glucose signal nor to a heat shock. Mutant strains, deficient in the UDPG-dependent enzyme but possessing the ADPG-dependent activity, did not accumulate trehalose either during growth or during sporulation. A diploid, homozygous for the deficiency in the UDPG-dependent synthase activity, was constructed. During sporulation the ADPG-dependent activity remained constant. However, when mutant spores were subjected to a glucose signal, trehalose was accumulated to the same extent as in control spores.
authors.affiliation: Depto. de Bioquimica, Universidade Federal do Rio de Janeiro, Brasil.
authors.forename: J C
authors.initials: JC
authors.lastname: Ferreira
authors.forename: A D
authors.initials: AD
authors.lastname: Panek
mesh_terms: D000245:Adenosine Diphosphate Glucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013172:Spores, Fungal / Q000378:metabolism; D014198:Trehalase / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
chemical_list: D000245:Adenosine Diphosphate Glucose; D014199:Trehalose; C087400:ADPG-dependent trehalose-6-phosphate synthase; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase; D014198:Trehalase; D005947:Glucose