Studies into the mechanism for MHV transcription.
Previous studies have demonstrated that the MHV genome is divided into seven transcriptional units which are transcribed from highly conserved intergenic start sites (UCU/CAAAC) into mRNA containing a common leader RNA at the 5' end and a coterminal 3' end. In this manuscript, we provide evidence that an additional transcriptional unit is encoded at the 3' end of the MHV genome and is transcribed from a perfect intergenic region into a leader-containing approximately 800 nt mRNA. This mRNA could potentially encode a small 17-18 kDa protein which is identical to the C-terminal third of the nucleocapsid gene.
Schaad
M C
MC
Department of Epidemiology, University of North Carolina at Chapel Hill 27599-7400.
Chen
W
W
Peel
S A
SA
Baric
R S
RS
D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics; D016384:Consensus Sequence; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
D012333:RNA, Messenger; D012367:RNA, Viral; D014758:Viral Core Proteins; D015678:Viral Structural Proteins