N-acetylneuraminic acid plays a critical role for the haemagglutinating activity of avian infectious bronchitis virus and porcine transmissible gastroenteritis virus.
Porcine transmissible gastroenteritis virus (TGEV) was found to resemble avian infectious bronchitis virus (IBV) in its interaction with erythrocytes. Inactivation of the receptors on erythrocytes by neuraminidase treatment and restoration of receptors by reattaching N-acetylneuraminic acid (Neu5Ac) to cell surface components indicated that alpha 2,3-linked Neu5Ac serves as a receptor determinant for TGEV as has been reported recently for IBV. Similar to IBV, the haemagglutinating activity of TGEV is evident only after pretreatment of virus with neuraminidase indicating that inhibitors on the virion surface have to be inactivated in order to induce the HA-activity of these viruses. A model is presented to explain why the HA-activity of untreated virus is masked and how neuraminidase treatment results in the unmasking of this activity.
Schultze
B
B
Institut für Virologie, Philipps-Universität Marburg, Germany.
Enjuanes
L
L
Cavanagh
D
D
Herrler
G
G
D001426:Bacterial Proteins / Q000494:pharmacology; D002240:Carbohydrate Sequence; D004910:Erythrocyte Membrane / Q000378:metabolism; D006387:Hemagglutination, Viral / Q000502:physiology*; D001351:Infectious bronchitis virus / Q000187:drug effects / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D000085064:Receptors, Coronavirus; D011991:Receptors, Virus / Q000378:metabolism; D012794:Sialic Acids / Q000502:physiology*; D064370:Spike Glycoprotein, Coronavirus; D005760:Transmissible gastroenteritis virus / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism
D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D000085064:Receptors, Coronavirus; D011991:Receptors, Virus; D012794:Sialic Acids; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid