Suppression of prolactin and cytotoxic T-lymphocyte activity in PCB-treated mice.
Halogenated aromatic hydrocarbons (HAH) are ubiquitous environmental contaminants. Studies in rats have shown that HAH treatment can lead to dysregulation of circulating hormone levels, including prolactin. Reduction of prolactin levels in both rats and mice is inhibitory to immune function. Previous studies have reported suppression of alloantigen-specific cytotoxic T-lymphocyte (CTL) activity in mice treated with 3,3', 4,4', 5,5'-hexachlorobiphenyl (HxCB). Here we report that treatment of mice with HxCB (10 mg/kg body weight) leads to a significant reduction of serum prolactin levels (by 89% to 3.7 ng/ml) on day 10 post alloantigen injection (P815 mastocytoma), the day of peak alloantigen-specific CTL activity. Prolactin levels were not altered on day 3 post alloantigen injection. Treatment with bromocriptine (5 mg/kg/day) reduced serum prolactin levels slightly on day 3 and significantly (94% to 2.1 ng/ml) on day 10 post alloantigen injection. Splenic CTL activity was not altered by treatment with bromocriptine. The data presented here suggest that reduction of prolactin levels alone, to the extent observed in HxCB-treated mice, is not causative for CTL suppression.
De Krey
G K
GK
College of Veterinary Medicine, Oregon State University, Corvallis 97331.
Hollingshead
N C
NC
Kerkvliet
N I
NI
Smith
B B
BB
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007519:Isoantigens / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008962:Models, Theoretical; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011388:Prolactin / Q000097:blood / Q000187:drug effects*; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D007519:Isoantigens; D001971:Bromocriptine; D011388:Prolactin; D011078:Polychlorinated Biphenyls; C027309:3,4,5,3',4',5'-hexachlorobiphenyl