Preparation of isolated perfused ground squirrel brain.
This paper describes preparation of the isolated brain of hibernating ground squirrel maintained by intraarterial perfusion. This technique allows a long-term survival (about 3 days) of the isolated brain of adult animals. The preparation viability was assessed by extracellular investigation of stability of structure-specific electrical activity. The areas investigated include neocortex, hippocampus, thalamus, hypothalamus, and brain stem.
Pakhotin
P I
PI
Institute of Cell Biophysics, Academy of Sciences of Russia, Moscow District.
Pakhotina
I D
ID
D000818:Animals; D001921:Brain; D002555:Cerebrospinal Fluid; D006605:Hibernation; D006652:Histological Techniques; D066298:In Vitro Techniques; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D012589:Sciuridae