title: Bacitracin production by a new strain of Bacillus subtilis. Extraction, purification, and characterization.
abstract: A new strain of Bacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth of Micrococcus flavus. The production of the antibiotic in culture medium followed to extraction with n-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.
authors.affiliation: Mestrado de Ciencias Farmacêuticas, Universidade Federal de Pernambuco, Recife-PE, Brazil.
authors.forename: E C
authors.initials: EC
authors.lastname: Azevedo
authors.forename: E M
authors.initials: EM
authors.lastname: Rios
authors.forename: K
authors.initials: K
authors.lastname: Fukushima
authors.forename: G M
authors.initials: GM
authors.lastname: Campos-Takaki
mesh_terms: D020001:1-Butanol; D001412:Bacillus subtilis / Q000378:metabolism*; D001414:Bacitracin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000440:Butanols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003470:Culture Media; D008826:Microbial Sensitivity Tests; D008837:Micrococcus / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000440:Butanols; D003470:Culture Media; D001414:Bacitracin; D020001:1-Butanol