title: Reperfusion injury and exhaled hydrogen peroxide.
abstract: Reactive oxygen species have been implicated in the pathophysiology of lung injury associated with the sequence of ischemia-reperfusion. To study this, we measured the exhaled breath hydrogen peroxide concentration [H2O2] in human and canine models of reperfusion lung injury. Our models were patients subjected to cardiopulmonary bypass (CPB) (Group 1), patients undergoing pulmonary thromboendarterectomy (Group 2), canine single lung transplant (Group 3), and patients subjected to peripheral ischemia resulting from aortic cross-clamping or tourniquet application (Group 4). In addition, we studied two groups with severe lung injury as positive controls. These consisted of hydrochloric acid (HCl)-induced canine lung injury (Group 5) and patients with adult respiratory distress syndrome (Group 6). The exhaled H2O2 was collected by using a -2 degrees C glass coil and assayed by a spectrophotometric method. In Group 1 samples were collected before and immediately after CPB. Group 2 samples were obtained before CPB, immediately after CPB, 3 h later, and daily until extubation. Samples in Group 3 were collected before lung transplant, and hourly for 3 h beginning immediately afterward. Group 4 samples were collected at the onset of reperfusion. Samples from Group 5 were collected before HCl and after HCl injury, at 0.5-1.5 and 2-3 h. Group 6 samples were collected when criteria for adult respiratory distress syndrome were met. Groups 1, 3, and 4 exhibited no significant increases in exhaled [H2O2] compared to control values. Group 2 had significantly increased [H2O2] (5.59 +/- 3.07 x 10(-7) mol/L, P = 0.028) on postoperative Day 2, but there was no correlation of [H2O2] with physiologic indicators of lung injury.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anesthesiology, University of California at San Diego Medical Center 92103-8770.
authors.forename: W C
authors.initials: WC
authors.lastname: Wilson
authors.forename: P R
authors.initials: PR
authors.lastname: Laborde
authors.forename: J L
authors.initials: JL
authors.lastname: Benumof
authors.forename: R
authors.initials: R
authors.lastname: Taylor
authors.forename: J F
authors.initials: JF
authors.lastname: Swetland
mesh_terms: D000818:Animals; D004285:Dogs; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015427:Reperfusion Injury / Q000503:physiopathology*; D012119:Respiration / Q000502:physiology*
chemical_list: D006861:Hydrogen Peroxide