Short-term starvation increases calcidiol-24-hydroxylase activity and mRNA level in rat kidney.
The renal mitochondrial calcidiol-24-hydroxylase activity and the corresponding cytochrome P-450 mRNA level were measured in rats subjected to short-term starvation alone or in combination with calcitriol treatment. Short-term starvation of 24 and 48 h increased the mRNA level by five- and six-fold, respectively. The 24-hydroxylase activity increased by five- and threefold, respectively. Treatment with calcitriol markedly increased the enzyme activity about 20-fold and the mRNA level about six-fold. In rats subjected to calcitriol treatment combined with 24 h of starvation, a significant further increase in enzyme activity was observed. The mRNA levels increased but the difference was not significant statistically. The results indicate that the mechanism by which starvation stimulates the enzymes is different, at least in part, from that behind the stimulatory effect of calcitriol.
Hagenfeldt-Pernow
Y
Y
Department of Clinical Chemistry I, Karolinska Institutet, Huddinge University Hospital, Sweden.
Ohyama
Y
Y
Sudjana-Sugiaman
E
E
Okuda
K
K
Björkhem
I
I
D000818:Animals; D002117:Calcitriol / Q000097:blood / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D007668:Kidney / Q000201:enzymology*; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013217:Starvation / Q000201:enzymology*; D013250:Steroid Hydroxylases / Q000096:biosynthesis* / Q000235:genetics; D065668:Vitamin D3 24-Hydroxylase
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D065668:Vitamin D3 24-Hydroxylase; D002117:Calcitriol