title: The effect of protein kinase C modulators on endogenous protein phosphorylation in primate kidney.
abstract: The effects of modulators of protein kinase C on protein phosphorylation were studied in primate kidney. Phosphorylation of specific proteins, investigated using electrophoresis and autoradiography, indicated that in the absence of modulators eight major proteins were phosphorylated in membrane of baboon kidney; modulators enhanced a possible endogenous substrate of 41.8 +/- 2.3 kDa. In cytosol, eight major proteins were phosphorylated in the absence of modulators; a band of mass 110.4 +/- 13.8 kDa was enhanced with modulators added. Similar results were found in human kidney cytosol.
authors.affiliation: Department of Medical Biochemistry, University of the Witwatersrand, Parktown, Johannesburg, South Africa.
authors.forename: N
authors.initials: N
authors.lastname: Laminski
authors.forename: K
authors.initials: K
authors.lastname: Hammond
authors.forename: A
authors.initials: A
authors.lastname: Meyers
mesh_terms: D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004075:Diglycerides / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D010215:Papio; D010718:Phosphatidylserines / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D010718:Phosphatidylserines; D011506:Proteins; C044295:1,2-dioctanoylglycerol; D011493:Protein Kinase C; D002118:Calcium