title: Nonnuclear effects of the steroid hormone 1 alpha,25(OH)2-vitamin D3: analogs are able to functionally differentiate between nuclear and membrane receptors.
abstract: The steroid hormone 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3] stimulates biological responses via both genomic mechanisms and nongenomic mechanisms (opening of voltage-gated Ca2+ channels). We report here that the two closed B-ring steroid analogs of 1 alpha,25(OH)2D3, 1 alpha,25(OH)2-7-dehydrocholesterol and 1 alpha,25(OH)2-lumisterol3, are able to generate the nongenomic response, transcaltachia, without the ability to compete with the natural metabolite for binding to its nuclear receptor. We propose that the nongenomic membrane associated receptor can accept the ligand in its closed "6-s-cis" conformation whereas the nuclear receptor prefers the extended "6-s-trans" conformer.
authors.affiliation: Department of Biochemistry, University of California, Riverside 92521.
authors.forename: M C
authors.initials: MC
authors.lastname: Dormanen
authors.forename: J E
authors.initials: JE
authors.lastname: Bishop
authors.forename: M W
authors.initials: MW
authors.lastname: Hammond
authors.forename: W H
authors.initials: WH
authors.lastname: Okamura
authors.forename: I
authors.initials: I
authors.lastname: Nemere
authors.forename: A W
authors.initials: AW
authors.lastname: Norman
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D004386:Duodenum / Q000378:metabolism; D008297:Male; D018167:Receptors, Calcitriol / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014809:Vitamin D-Binding Protein / Q000378:metabolism
chemical_list: D018167:Receptors, Calcitriol; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D014809:Vitamin D-Binding Protein; D002117:Calcitriol; D002118:Calcium