In vivo viability studies of two additive solutions in the postthaw preservation of red cells held for 3 weeks at 4 degrees C.
An optimized additive solution was developed for the postthaw preservation of red cells that contained adenine, glucose, disodium phosphate, and citrate buffer. This solution, called AS-17, was compared to AS-3 solution in a clinical trial using 40 subjects (20 in each arm). Fresh-frozen red cells were thawed and deglycerolized after 1 to 18 months and subjected to a second period of storage in either solution for up to 3 weeks at refrigerator temperatures. Both solutions yielded red cells with 24-hour survivals in excess of 75 percent. Cells stored in AS-3 for 21 days had a mean survival of 77 +/- 8 percent and cells stored in AS-17 a mean survival of 79 +/- 11 percent. The AS-17 solution resulted in improved maintenance of pH, p50, and 2,3 DPG compared to that with AS-3, but both solutions appear adequate for 3 weeks of postthaw storage.
Moore
G L
GL
Blood Research Division, Letterman Army Institute of Research, Presidio of San Francisco, California.
Hess
J R
JR
Ledford
M E
ME
D019794:2,3-Diphosphoglycerate; D000225:Adenine; D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D001793:Blood Preservation; D002951:Citrates; D019343:Citric Acid; D015925:Cryopreservation; D004163:Diphosphoglyceric Acids; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000502:physiology*; D005947:Glucose; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates; D012996:Solutions
D002951:Citrates; D004163:Diphosphoglyceric Acids; D010710:Phosphates; D012996:Solutions; D019794:2,3-Diphosphoglycerate; D019343:Citric Acid; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine; C018279:sodium phosphate