title: Cloning and sequence analysis of the spike gene from several feline coronaviruses.
abstract: The DNA sequence encoding the spike gene from the DF2 strain of Type II feline infectious peritonitis virus (FIPV), a temperature sensitive FIPV virus (TS-DF2) and an isolate of feline enteric coronavirus (FECV 1683) were determined. Comparison of the published WSU 1146 and DF2 FIPV S genes showed that the viruses shared a high degree of homology (99.6%). Likewise, the S gene of the virulent DF2 FIPV virus was closely conserved to that isolated from the vaccine virus strain, TS-DF2 FIPV. In contrast, the FECV S gene had numerous DNA and amino acid differences when compared to the virulent FIPV sequences. Sequence differences among the feline coronavirus isolates were localized to the amino-terminus region of the S gene.
authors.affiliation: Department of Molecular Biology, SmithKline Beecham Animal Health, King of Prussia, Pennsylvania 19406-0939.
authors.forename: A P
authors.initials: AP
authors.lastname: Reed
authors.forename: S
authors.initials: S
authors.lastname: Klepfer
authors.forename: T
authors.initials: T
authors.lastname: Miller
authors.forename: E
authors.initials: E
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017934:Coronavirus / Q000235:genetics*; D016765:Coronavirus, Feline / Q000235:genetics; D005814:Genes, Viral; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins