[Comparative study of generation of active forms of oxygen in blood leukocytes and alveolar macrophages in patients with bronchial asthma].
The aim of the study was to perform comparative simultaneous study of generation of active forms of oxygen by blood leukocytes (GAFBL) and generation of active forms of oxygen (GAFO) by alveolar macrophages (AM) and to evaluate their contribution in development of bronchial asthma. The study comprised 25 subjects: 10 with bronchial asthma, 10 with chronic bronchitis and 5 healthy subjects. GAFO AM and GAFBL were examined by method of luminol-dependent chemiluminescence (CL). The basal and stimulated CL parameters as well as the relationship of GAFBL and GAFO AM were measured. In the phase of deterioration, both CL parameters, GAFBL and GAFO AM were markedly increased in patients with bronchial asthma compared to normal subjects. However, in healthy subjects and in patients with bronchial asthma both CL parameters of GAFBL were markedly increased than the analogous parameters of AM. The relationship of GAFBL and GAFO AM for both parameters in patients with bronchial asthma was lower than in healthy subjects which prove AM to be more active than blood leukocytes. In patients with chronic bronchitis both CL parameters of GAFO AM were markedly increased than in healthy subjects and only the stimulated CL parameters of GAFBL were lower than in patients with bronchial asthma. However, the relationship of GAFBL and GAFO AM for the basal CL parameters practically do not differ from the normal which suggests the larger activity of blood leukocytes compared to AM activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Boljevic
S
S
Katedra za unutrasnje bolesti br. 2 Prvog medicinskog fakulteta i Katedra za patofiziologiju Moskovske medicinske akademije.
Daniljak
I G
IG
Kogan
A H
AH
D000328:Adult; D001249:Asthma / Q000378:metabolism*; D005260:Female; D005609:Free Radicals; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008163:Luminescent Measurements; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000378:metabolism*
D005609:Free Radicals; D010100:Oxygen
Uporedno proucavanje stvaranja aktivnih formi kiseonika u leukocitima krvi i alveolarnim makrofagima kod bolesnika sa bronhijalnom astmom.