Prohibitin expression during cellular senescence of human diploid fibroblasts.
Prohibitin is an evolutionarily conserved gene postulated to possess tumor suppressor activity and to contribute to the limited lifespan of human diploid fibroblast-like cells. Prohibitin mRNA and protein expression and its ability to become post-translationally modified were determined in human diploid fibroblast-like cells of different in vitro ages. The expression of prohibitin mRNA and protein changes little with increasing in vitro age; however, its protein product is post-synthetically modified in younger but not older cells. These results suggest that prohibitin is similar to the retinoblastoma gene product whose anti-proliferative activity remains active in older cells because it is not post-synthetically modified.
Liu
X T
XT
Noble Center for Biomedical Research, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Stewart
C A
CA
King
R L
RL
Danner
D A
DA
Dell'Orco
R T
RT
McClung
J K
JK
D002453:Cell Cycle; D002478:Cells, Cultured; D016922:Cellular Senescence; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D000091402:Prohibitins; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins
D000091402:Prohibitins; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins