The salting-out test to identify virulent Yersinia pseudotuberculosis.
A total of 54 strains including 8 serotypes of Yersinia pseudotuberculosis were examined to find out about their cell surface hydrophobicity and virulence plasmid-associated properties. All the strains which aggregated in 0.9% saline expressed the properties of calcium-dependent growth and autoagglutination. However, the hydrophilic strains did not possess virulence plasmid-associated properties. The above results suggest that the salting-out test with 0.9% saline could be an effective measure to differentiate the virulence plasmid-carrying strains of Y. pseudotuberculosis from those of plasmidless virulence.
Tomita
M
M
Yamaguchi Prefectural Research Institute of Health, Japan.
Matsusaki
S
S
Katayama
A
A
Endo
R
R
Miyamura
S
S
D000818:Animals; D008297:Male; D051379:Mice; D008828:Microbiological Techniques; D010957:Plasmids; D012492:Salts; D014774:Virulence; D014867:Water; D015011:Yersinia pseudotuberculosis / Q000302:isolation & purification / Q000472:pathogenicity*
D012492:Salts; D014867:Water