Geographical origin of an introduced insect pest, Listronotus bonariensis (Kuschel), determined by RAPD analysis.
The Argentine stem weevil, Listronotus bonariensis (Kuschel) (Coleoptera: Curculionidae), is an important introduced pasture pest in New Zealand. In this study geographical populations of this species were analysed using polymerase chain reaction-based randomly amplified polymorphic DNA (RAPD), in an attempt to determine the geographical origin of the pest. Morphologically indistinguishable individuals were collected from nine South American, five New Zealand and one Australian populations. Ten primers were screened for usefulness, two of which revealed significant, scorable polymorphisms between these populations. The results indicated that the sampled New Zealand L. bonariensis populations originated from the east coast of South America.
Williams
C L
CL
Centre for Molecular Biology, Lincoln University, Canterbury, New Zealand.
Goldson
S L
SL
Baird
D B
DB
Bullock
D W
DW
D000818:Animals; D001118:Argentina; D001483:Base Sequence; D001938:Brazil; D001517:Coleoptera / Q000235:genetics; D017931:DNA Primers; D005784:Gene Amplification; D008969:Molecular Sequence Data; D009520:New Zealand; D011110:Polymorphism, Genetic
D017931:DNA Primers