Metabolic studies of a fructose-intolerant yeast by in vivo 31P-nuclear magnetic resonance spectroscopy.
The metabolic effects of the administration of fructose to a yeast expressing the cDNA for rat liver ketohexokinase have been investigated by 31P-nuclear magnetic resonance spectroscopy. Cessation of growth suffered by the yeast on exposure to 5 and 25 mM-fructose was accompanied by a large accumulation of fructose 1-phosphate at the expense of cytoplasmic orthophosphate and nucleoside triphosphate. Shifts in resonances were consistent with a drop in cytoplasmic pH. Arresting growth with 1 mM-fructose, however, did not result in these changes, although a large accumulation of fructose 1-phosphate occurred which may have been supported by the mobilization of polyphosphates.
Doyle
T C
TC
Department of Biochemistry, University of Oxford, U.K.
Spickett
C M
CM
Donaldson
I A
IA
Ratcliffe
R G
RG
D000818:Animals; D005631:Fructokinases / Q000096:biosynthesis* / Q000235:genetics; D005632:Fructose / Q000494:pharmacology*; D005633:Fructose Intolerance; D009682:Magnetic Resonance Spectroscopy; D010759:Phosphorus Isotopes; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D014170:Transformation, Genetic
D010759:Phosphorus Isotopes; D011994:Recombinant Proteins; D005632:Fructose; D005631:Fructokinases; C021475:ketohexokinase