Rate of alteration of hepatic mixed-function oxidase system in rats fed different dietary fats.
Studies were carried out to evaluate and relate the rate of alteration in mixed-function oxidase system with the changes of the fatty acid composition of rat microsomes induced by different dietary lipids. Male weanling rats were fed from day 21 to 120 with a commercial rat diet or a semisynthetic diet containing no fat or 10% fat consisting of peanut-rapeseed oil, sunflower oil, or salmon oil. In rats fed a fat-free diet, the cytochrome P-450 concentration and aniline hydroxylase, aminopyrine N-demethylase, and NADPH-cytochrome-c reductase activities of liver microsomes at 120 days were, respectively, 26, 16, 10, and 24% lesser than those of rats fed the control diet. However, cytochrome b5 concentration and NADH-cytochrome-b5 reductase activity were, respectively, 33 and 43% higher than those of the control group at the same time. When rats were fed the sunflower oil diet, the cytochrome P-450 concentration and NADH-cytochrome-b5 reductase activity at 120 days were, respectively, 11 and 23% lesser than those of control group. But the cytochrome b5 concentration was 10% higher than that of the control group. In rats fed the fish oil diet, the cytochrome P-450 concentration and NADPH-cytochrome-c reductase, aniline hydroxylase, and aminopyrine N-demethylase activities at 120 days were, respectively, 30, 48, 41, and 31% higher than those of rats fed the control diet. These enzymes were correlated very well (0.84 < r < 0.93), P < 0.05 with dietary sigma polyunsaturated fatty acids (n-3).(ABSTRACT TRUNCATED AT 250 WORDS)
Ammouche
A
A
Institut national de la santé et de la recherche médicale, Unité 26, Hôpital Fernand Widal, France.
Dinh
L
L
Youyou
A
A
Clément
M
M
Bourre
J M
JM
D000633:Aminopyrine N-Demethylase / Q000032:analysis; D000815:Aniline Hydroxylase / Q000032:analysis; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D003579:Cytochrome Reductases / Q000032:analysis; D042966:Cytochrome-B(5) Reductase; D015786:Cytochromes b5 / Q000032:analysis; D004041:Dietary Fats / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis*; D005395:Fish Oils / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry* / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000032:analysis*; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D010938:Plant Oils / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014886:Weaning
D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D010938:Plant Oils; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase; D009251:NADPH-Ferrihemoprotein Reductase