Cloning and DNA sequence of a 29 kilodalton polypeptide gene of Mycoplasma gallisepticum as a possible protective antigen.
A lambda gt11 clone, designated M1 and having a 0.8 kilobase (kb) insert, was selected by screening a Mycoplasma gallisepticum (M.g.) genomic DNA library with antisera against M.g. cells and their membrane proteins. The sequence of a 1.7 kb EcoRI fragment of genomic DNA covering the entire M1 insert revealed a long open reading frame, TM-1, that encoded a polypeptide with a deduced molecular weight of 29 kDa. An antiserum raised in chicken against the TM-1 polypeptide, which was produced by recombinant Escherichia coli cells and purified by column chromatography, inhibited growth of M.g. cells in vitro. Moreover, chickens immunized with this polypeptide were partially protected from a challenge with virulent M.g. This polypeptide may serve as the basis for a vaccine against M.g. infection.
Saito
S
S
Life Science Laboratory, Research and Development Center, Nippon Zeon Co., Ltd., Kawasaki, Japan.
Fujisawa
A
A
Ohkawa
S
S
Nishimura
N
N
Abe
T
T
Kodama
K
K
Kamogawa
K
K
Aoyama
S
S
Iritani
Y
Y
Hayashi
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D003062:Codon; D004269:DNA, Bacterial / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D005798:Genes, Bacterial; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009174:Mycoplasma / Q000235:genetics / Q000276:immunology*
D000942:Antigens, Bacterial; D003062:Codon; D004269:DNA, Bacterial; D007106:Immune Sera