Preparation and characterization of interleukin-2-gelonin conjugates made using different cross-linking reagents.
Conjugates of IL-2 with the ribosome-inactivating protein gelonin were prepared using heterobifunctional reagents to link the proteins via disulfide, acid-labile, and noncleavable linkers. In each case, one protein was modified using 2-iminothiolane. The sulfhydryl groups so introduced were then reacted either with 2-nitro-5-dithiobenzoate groups or with iodoacetamido groups which had been introduced into the second protein. In the case of the acid-labile linkage, a reagent which forms a labile bond upon reaction with amino groups, 4-(iodoacetamido)-1-cyclohexene-1,2-dicarboxylic acid anhydride (its synthesis is described in this paper) was used to modify the toxin. The conjugates were separated from nonconjugated proteins by gel filtration on Sephadex G100 (SF). Each was analyzed with respect to its ribosome-inactivating activity, its ability to bind to the IL-2 receptor, and its in vitro cytotoxicity. The ribosome-inactivating activity of gelonin was unaffected by modification with 2-iminothiolane and was retained in conjugates prepared using this reagent. Modification of the toxin with 4-(iodoacetamido)-1-cyclohexene-1,2-dicarboxylic acid anhydride to form the acid-labile link drastically reduced the activity of the toxin. However, the activity of the toxin was recovered following acid treatment to release the native protein. Conjugates containing each type of linkage exhibited both specific binding and selective cytotoxicity toward cells expressing the IL-2 receptor. The most potent of these toxins, that containing the disulfide linkage, exhibited a cytotoxicity which was 2 orders of magnitude greater than that of unconjugated gelonin.
McIntyre
G D
GD
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Scott
C F
CF
Ritz
J
J
Blättler
W A
WA
Lambert
J M
JM
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000502:physiology; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004220:Disulfides / Q000138:chemical synthesis; D005260:Female; D007376:Interleukin-2 / Q000138:chemical synthesis* / Q000378:metabolism / Q000633:toxicity; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D010940:Plant Proteins / Q000138:chemical synthesis* / Q000378:metabolism / Q000633:toxicity; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology
D003432:Cross-Linking Reagents; D004220:Disulfides; D007376:Interleukin-2; D010940:Plant Proteins; D015375:Receptors, Interleukin-2; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum