[MultiCycle software for cell cycle analyses].
MultiCycle software (M-cycle), a computer cell cycle analysis program that has a background debris and aggregation compensating function, was utilized in this study to prove the usefulness of the M-cycle. The S phase fraction (SPF) calculated by the M-cycle was compared to that of bromodeoxyuridine labelling index (BLI) using colorectal carcinoma cell lines. The SPF value was slightly lower using the M-cycle than that of the BLI in Colo 201 and Colo 320 and lower significantly in Widr. This may indicate that the M-cycle effectively compensated for the background existing in the DNA histogram. The SPF value was computed both by the M-cycle and the sum of broadened rectangles model (SOBR). The SPF value of these cell lines showed a lower figure in the M-cycle than in the SOBR. The SPF value of paraffin-embedded material through the M-cycle and the SOBR was compared according to DNA ploidy patterns. The SPF value computed by the M-cycle was significantly lower in both ploidy patterns than that of the SOBR. In conclusion, the M-cycle is a useful tool for cell cycle analyses of simple DNA flow cytometric histograms obtained by paraffin-embedded material.
Funai
S
S
First Dept. of Surgery, Kinki University School of Medicine.
Kurooka
K
K
Fujii
Y
Y
Inufusa
H
H
Yamada
H
H
Imanishi
Y
Y
Sato
S
S
Shindo
K
K
Yasutomi
M
M
D002453:Cell Cycle; D015179:Colorectal Neoplasms / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D016612:Paraffin Embedding; D011003:Ploidies; D016196:S Phase; D012984:Software
D004273:DNA, Neoplasm