Use of polymerase chain reaction to detect porcine parvovirus associated with swine embryos.
The role of porcine parvovirus (PPV) in inducing reproductive failure in swine has been extensively documented. However, information is not available as to the risk of PPV transmission by embryo transfer. Using the polymerase chain reaction (PCR) technique, PPV-specific DNA was detected in association with 4-day-old porcine embryos incubated in vitro in the presence of NADL-8 strain of PPV, despite attempts to rid the embryos of virus by either washing or treatment with pronase or trypsin. The presence of PPV in embryos collected from acutely infected swine was not detected by PCR, although PPV DNA was detected in the proximal portion of the reproductive tract during the early stages of infection. Viral-specific nucleic acid was not detected in embryos transferred from infected donors to seronegative recipients and retrieved and assayed on the 15th and 32nd days of gestation. Results of the use of PCR to detect PPV associated with swine female reproductive tract and embryos ascribe minimal risk to the transmission of PPV to seronegative recipients through embryo transfer.
Gradil
C M
CM
Animal Diseases Research Institute, Agriculture Canada, Nepean, Ontario.
Harding
M J
MJ
Lewis
K
K
D000034:Abortion, Veterinary; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D004622:Embryo, Mammalian / Q000382:microbiology*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases
D017931:DNA Primers; D004279:DNA, Viral