Quantification of the regulation of glycerol and maltose metabolism by IIAGlc of the phosphoenolpyruvate-dependent glucose phosphotransferase system in Salmonella typhimurium.
The amount of IIAGlc, one of the proteins of the phosphoenolpyruvate:glucose phosphotransferase system (PTS), was modulated over a broad range with the help of inducible expression plasmids in Salmonella typhimurium. The in vivo effects of different levels of IIAGlc on glycerol and maltose metabolism were studied. The inhibition of glycerol uptake, by the addition of a PTS sugar, was sigmoidally related to the amount of IIAGlc. For complete inhibition of glycerol uptake, a minimal ratio of about 3.6 mol of IIAGlc to 1 mol of glycerol kinase (tetramer) was required. Varying the level of IIAGlc (from 0 to 1,000% of the wild-type level) did not affect the growth rate on glycerol, the rate of glycerol uptake, or the synthesis of glycerol kinase. In contrast, the growth rate on maltose, the rate of maltose uptake, and the synthesis of the maltose-binding protein increased two- to fivefold with increasing levels of IIAGlc. In the presence of cyclic AMP, the maximal levels were obtained at all IIAGlc concentrations. The synthesis of the MalK protein, the target of IIAGlc, was not affected by varying the levels of IIAGlc. The inhibition of maltose uptake was sigmoidally related to the amount of IIAGlc. For complete inhibition of maltose uptake by a PTS sugar, a ratio of about 18 mol of IIAGlc to 1 mol of MalK protein (taken as a dimer) was required.
van der Vlag
J
J
E. C. Slater Institute, University of Amsterdam, The Netherlands.
van Dam
K
K
Postma
P W
PW
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000096:biosynthesis; D015971:Gene Expression Regulation, Enzymologic; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000032:analysis; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008320:Maltose / Q000378:metabolism*; D058619:Maltose-Binding Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000032:analysis; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; C068021:MalK protein, Bacteria; C489399:MalK protein, Salmonella typhimurium; D058619:Maltose-Binding Proteins; D007544:Isopropyl Thiogalactoside; D008320:Maltose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase; D005991:Glycerol Kinase; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022360:phosphoenolpyruvate-protein phosphotransferase; D005990:Glycerol