Evidence for transcapillary transport of reduced glutathione in vascular perfused guinea-pig brain.
Using a vascular brain perfusion model in the guinea-pig, the net uptake of [35S]-GSH by the brain was found to be linear and similar in various regions during 10 min perfusion. Dual labeled [35S and 3H] GSH taken up by the brain had the same isotope ratio as the injected stock whether or not gamma- glutamyl transferase was inhibited. Greater than 96% of brain uptake of [35S]-cysteine-labeled GSH and [3H]-glycine-labeled GSH were in intact form. Transcytosis of [35S]-GSH from lumen into brain parenchyma was demonstrated using a capillary depletion technique. Both GSH and GSH-monoethyl ester inhibited [35S]-GSH transport. Thus, we have demonstrated blood-brain barrier extraction of circulating GSH in a brain perfusion model, and the transcytosis of intact GSH into the brain parenchyma without breakdown.
Zlokovic
B V
BV
Department of Neurological Surgery, Children's Hospital, Los Angeles, USC School of Medicine 90033.
Mackic
J B
JB
McComb
J G
JG
Weiss
M H
MH
Kaplowitz
N
N
Kannan
R
R
D000818:Animals; D001692:Biological Transport; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002196:Capillaries / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006168:Guinea Pigs; D008297:Male
D005978:Glutathione