title: Induction of inflammatory cytokines by Nocardia fractions.
abstract: NDCM and NLD fractions of Nocardia opaca cell walls were used for in vitro stimulation of human and porcine peripheral blood mononuclear cells. Inflammatory cytokines IL-1, IL-6, tumour necrosis factor (TNF) alpha and interferon (IFN) gamma were detected at the single-cell level using paraformaldehyde-fixed and saponin-permeabilized mononuclears stained with cytokine-specific antibodies and indirect immunofluorescence method. IL-1, IL-6 and TNF were produced by human monocytes stimulated for 2 h, IFN gamma-positive lymphocytes were detected later. IFN gamma was produced also by activated porcine lymphocytes. Cells expressing apoptotic features were found among blood mononuclears treated with Nocardia fractions.
authors.affiliation: Institute of Microbiology, Department of Immunology and Gnotobiology, Nový Hrádek.
authors.forename: I
authors.initials: I
authors.lastname: Trebichavský
authors.forename: R
authors.initials: R
authors.lastname: Barot-Ciorbaru
authors.forename: B
authors.initials: B
authors.lastname: Charley
authors.forename: I
authors.initials: I
authors.lastname: Splíchal
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability; D017358:Cell Wall Skeleton / Q000276:immunology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007378:Interleukins / Q000096:biosynthesis*; D008070:Lipopolysaccharides; D008934:Mitogens / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D009615:Nocardia / Q000276:immunology* / Q000648:ultrastructure; D009930:Organic Chemicals; D013552:Swine; D013556:Swine, Miniature; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D017358:Cell Wall Skeleton; D007378:Interleukins; D008070:Lipopolysaccharides; D008934:Mitogens; C101327:Nocardia delipidated cell mitogen; D009930:Organic Chemicals; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma