title: Bovine conglutinin gene exon structure reveals its evolutionary relationship to surfactant protein-D.
abstract: Bovine conglutinin (BC), a member of the mammalian C-type collectin subfamily, is a serum protein synthesized in liver that is believed to play a role in natural host defense. Previously, we have characterized a full length BC cDNA and we now describe the partial characterization of a genomic clone that encodes for the BC gene (CGN1). BC is encoded by nine exons spanning > 11 kb and has been localized previously to band 18 of bovine (Bos taurus) chromosome 28. Genomic sequencing demonstrated that the signal peptide/amino-terminal domain, the carbohydrate recognition domain, and the linking peptide, a domain between the collagenous region and the carbohydrate recognition domain, are each encoded by a single exon. The collagenous domain is split into five exons, with the 5' most region being located within the exon that also encodes the signal peptide/amino terminus. The remaining four collagenous domain exons are tandemly arranged with lengths of 117, 108, 108, and 117 bp, respectively. Overall, the BC genomic organization is very similar to that of the human surfactant protein-D gene, SFTP4. On the basis of identical collagen domain structures, we suggest that conglutinin and bovine surfactant protein-D evolved from a gene duplication event occurring in Bovidae after divergence from other mammals.
authors.affiliation: Department of Medicine, Boston University School of Medicine, Boston City Hospital, MA 02118.
authors.forename: L S
authors.initials: LS
authors.lastname: Liou
authors.forename: R
authors.initials: R
authors.lastname: Sastry
authors.forename: K L
authors.initials: KL
authors.lastname: Hartshorn
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
authors.forename: T B
authors.initials: TB
authors.lastname: Okarma
authors.forename: A I
authors.initials: AI
authors.lastname: Tauber
authors.forename: K N
authors.initials: KN
authors.lastname: Sastry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002417:Cattle; D037222:Collectins; D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants / Q000235:genetics*; D012712:Serum Globulins / Q000235:genetics*
chemical_list: D037222:Collectins; D017931:DNA Primers; D006023:Glycoproteins; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants; D012712:Serum Globulins; C024014:conglutinin