Acetylation of core histones causes the unfolding of 30 nm chromatin fiber: analysis by agarose gel electrophoresis.
In the present work it was directly demonstrated that increased acetylation level of histones causes the decompactization of 30 nm chromatin fiber, as revealed by low-percentage agarose gel electrophoresis. In the light of obtained results the possible molecular mechanism of the decompactization of acetylated fiber is discussed.
Krajewski
W A
WA
Laboratory of Biochemistry, Russian Academy of Sciences, Moscow.
Panin
V M
VM
Razin
S V
SV
D000107:Acetylation; D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000648:ultrastructure*; D004587:Electrophoresis, Agar Gel; D006657:Histones / Q000737:chemistry*; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009707:Nucleosomes; D011487:Protein Conformation; D051381:Rats
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes