Simultaneous determination of ofloxacin, norfloxacin and ciprofloxacin in human hair by high-performance liquid chromatography and fluorescence detection.
A high-performance liquid chromatographic method for the simultaneous determination of ofloxacin, norfloxacin and ciprofloxacin in human hair is described. A reversed-phase C18 column and a fluorescence detector with switching fluorescence wavelengths were used together with solid-phase extraction of the drugs from hair dissolved in 1 M sodium hydroxide. Reproducibility and linearity studies yielded coefficients of variation of 0.2-2.2, 1.4-3.1 and 1.5-3.4%, and correlation coefficients of 1.000, 0.999 and 0.999 within the concentration range 0.3-100 ng/ml for ofloxacin, norfloxacin and ciprofloxacin, respectively. For validation, hair samples were obtained from six subjects who had been taking one or two of the three fluoroquinolones. Assuming a hair growth-rate of 1 cm per month fluoroquinolones could be detected in the hair section(s) that had grown approximately between the dates of drug administration and hair sampling.
Mizuno
A
A
Department of Pharmacology, Hamamatsu University School of Medicine, Japan.
Uematsu
T
T
Nakashima
M
M
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008875:Middle Aged; D009643:Norfloxacin / Q000032:analysis*; D015242:Ofloxacin / Q000032:analysis*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
D002939:Ciprofloxacin; D015242:Ofloxacin; D009643:Norfloxacin