title: Similar toxic effect of 1,3-bis(2-chloroethyl)-1-nitrosourea on lymphocytes from human subjects differing in the expression of glutathione transferase M1-1.
abstract: Sixteen healthy donors were investigated for the presence or absence of glutathione transferase (GST) M1-1 in lymphocytes by immunodetection with polyclonal antibodies against human GST M1-1. Nine out of 16 individuals (56%) were categorized as GST M1-1 positive. Phytohaemagglutinin stimulated lymphocytes from GST M1-1 positive and negative donors were treated with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and compared regarding inhibition of [3H]thymidine incorporation as a measure of cytotoxicity. No significant differences in the effect of BCNU were observed between the two groups, indicating that GST M1-1 is not an important resistance factor for BCNU.
authors.affiliation: Department of Oncology, Karolinska Hospital, Stockholm, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Jungnelius
authors.forename: M
authors.initials: M
authors.lastname: Ridderström
authors.forename: J
authors.initials: J
authors.lastname: Hansson
authors.forename: U
authors.initials: U
authors.lastname: Ringborg
authors.forename: B
authors.initials: B
authors.lastname: Mannervik
mesh_terms: D002330:Carmustine / Q000633:toxicity*; D015870:Gene Expression; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D010835:Phytohemagglutinins; D013936:Thymidine / Q000378:metabolism
chemical_list: D010835:Phytohemagglutinins; D005982:Glutathione Transferase; D002330:Carmustine; D013936:Thymidine