Escape from the antiproliferative effect of transforming growth factor-beta 1 in LLC-PK1 renal epithelial cells.
Transforming growth factor-beta 1 (TGF-beta 1) usually inhibits proliferation of epithelial cells. We find that LLC-PK1 renal tubular epithelial cells develop rapid in vitro resistance to the inhibitory effects of TGF-beta 1 and subsequently proliferate in response to TGF-beta 1. This unique response to TGF-beta 1 is not observed in another renal tubular epithelial cell line (MDCK cells). The proliferative response to TGF-beta 1 is additive to that produced by other growth factors. The proliferative response to TGF-beta 1 occurs despite an effect of TGF-beta 1 to suppress epidermal growth factor stimulated c-myc mRNA as determined by Northern analyses. These results suggest that LLC-PK1 cells develop rapid resistance to TGF-beta 1 inhibition of proliferation in vitro and that this resistance occurs despite continued suppression of c-myc mRNA.
Anderson
R J
RJ
Department of Medicine, Denver Veterans Affairs Medical Center, Colorado.
Sponsel
H T
HT
Kroll
D J
DJ
Jackson
S
S
Breckon
R
R
Hoeffler
J P
JP
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D006133:Growth Substances; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D004247:DNA; D013936:Thymidine