Specificity of vascular endothelial cell growth factor receptor ligand binding domains.
Vascular endothelial cell growth factor binds with high affinity to FLT and KDR, two homologous tyrosine kinase receptors expressed on vascular endothelial cells. Placental growth factor, a vascular endothelial cell growth factor homologue, also binds with high affinity to the extracellular domains of FLT but not to the extracellular region of KDR. Vascular endothelial cell growth factor binds competitively with placental growth factor to the extracellular ligand binding domains of FLT, indicating that both ligands probably complex to overlapping or identical regions of this receptor.
Kendall
R L
RL
Department of Biochemistry, Merck Research Laboratories, Rahway, NJ 07065.
Wang
G
G
DiSalvo
J
J
Thomas
K A
KA
D001665:Binding Sites; D001667:Binding, Competitive; D003432:Cross-Linking Reagents; D016228:Endothelial Growth Factors / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D008222:Lymphokines / Q000737:chemistry*; D000072483:Placenta Growth Factor; D010926:Placental Hormones / Q000737:chemistry*; D011257:Pregnancy Proteins / Q000737:chemistry*; D011518:Proto-Oncogene Proteins / Q000737:chemistry*; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000737:chemistry*; D017978:Receptors, Growth Factor / Q000737:chemistry*; D040262:Receptors, Vascular Endothelial Growth Factor; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1; D042442:Vascular Endothelial Growth Factors
D003432:Cross-Linking Reagents; D016228:Endothelial Growth Factors; D008024:Ligands; D008222:Lymphokines; C000608036:PGF protein, human; D010926:Placental Hormones; D011257:Pregnancy Proteins; D011518:Proto-Oncogene Proteins; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D000072483:Placenta Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D040262:Receptors, Vascular Endothelial Growth Factor; D040281:Vascular Endothelial Growth Factor Receptor-1