Biologically active recombinant human complement factor H: synthesis and secretion by the baculovirus system.
A complete cDNA clone (4.3 kb) encoding human complement factor H (hCFH; 155 kDa) has been cloned into the pVL1393 baculovirus expression vector and transfected into Spodoptera frugiperda Sf9 insect cells. A biologically active (92 +/- 15%) 140-kDa protein was secreted into the medium with a yield of more than 5 mg/liter. This is the first report of synthesis of biologically active recombinant hCFH in a heterologous system.
Sharma
A K
AK
Department of Biochemistry, University of Texas Health Science Center, Tyler 75710-2003.
Pangburn
M K
MK
D000818:Animals; D016367:Baculoviridae; D003179:Complement C3b / Q000378:metabolism; D017242:Complement Factor H / Q000096:biosynthesis* / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D009036:Moths; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism
C493495:CFH protein, human; D011994:Recombinant Proteins; D003179:Complement C3b; D017242:Complement Factor H