title: A time course study of the alterations in the development of the hamster cerebellar cortex after destruction of the overlying meningeal cells with 6-hydroxydopamine on the day of birth.
abstract: This study is a chronological analysis of 6-hydroxydopamine-induced alterations in development of the hamster cerebellar cortex. This treatment destroys the overlying meningeal cells, the sequelae of which include (i) a thinning of the external granular layer over the folial apices and a thickening in the region of the prospective fissures, reflecting a retardation of the growth of the cerebellar cortex, accompanied by displacement of the normally superficialmost GFAP-positive external granular layer cells into deeper parts of the external granular layer; (ii) a retardation of multiplication of Golgi epithelial cells which colonize the rostral third of the Purkinje cell layer so that their numbers decrease in the rostralmost folia; (iii) disturbed morphological and biochemical differentiation of the Golgi epithelial cells and their processes, the growing radial Bergmann glial fibres which detach from the pial surface and branch within the external granular layer, causing a failure in endfeet formation at the superficial glia limitans, loss of characteristic radial morphology, with the adoption of a multipolar form, and normal or increased GFAP expression and decreased S-100 expression; (iv) fragmentation of the external granular layer beyond P5 to P7 with loss of the regular lamination and foliation of the cerebellar cortex, characterized by a completely random distribution of fragments of Purkinje cell layer, molecular zone and internal granular layer. We conclude that the destruction of meningeal cells interferes with the establishment and stabilization of both the external granular layer and the secondary radial glial scaffold composed of Golgi epithelial cells, whose proliferation, growth and differentiation is subsequently disturbed. The failure to stabilize the external granular layer and to form a normal secondary radial glial scaffold is, in turn, responsible for the disruption of the regular laminar deposition of the neurons of the cerebellar cortex.
authors.affiliation: Anatomisches Institut, Universität Kiel, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Sievers
authors.forename: F W
authors.initials: FW
authors.lastname: Pehlemann
authors.forename: S
authors.initials: S
authors.lastname: Gude
authors.forename: M
authors.initials: M
authors.lastname: Berry
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects; D002525:Cerebellar Cortex / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D006224:Cricetinae; D005109:Extracellular Matrix / Q000187:drug effects; D006056:Golgi Apparatus / Q000187:drug effects / Q000648:ultrastructure; D008578:Meninges / Q000166:cytology / Q000187:drug effects*; D008647:Mesocricetus; D016627:Oxidopamine / Q000633:toxicity*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D016627:Oxidopamine