A rapid and sensitive determination of salmon calcitonin in solutions containing bovine serum albumin or gelatin.
A sensitive and selective reversed phase high performance liquid chromatographic method was introduced for the determination of salmon calcitonin (sCT) in solutions containing bovine serum albumin or gelatin. The method was based on the sensitive fluorescence detection of sCT by postcolumn derivatization with o-phthal(di)aldehyde. The sample was loaded onto a reversed phase column, purified by column switching and separated with linear gradient elution using the ion pair technique. A linear relationship was obtained between the peak height and the amount injected in the concentration range 1-40 ng. The detection limit was 10 ng/mL (S/N = 3) at a sample injection volume of 100 microL.
Fukuda
T
T
Tsumura & Co., Ibaraki, Japan.
Yun
A
A
Imai
K
K
D000818:Animals; D002116:Calcitonin / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005780:Gelatin / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D009764:o-Phthalaldehyde
D012710:Serum Albumin, Bovine; D009764:o-Phthalaldehyde; C028815:salmon calcitonin; D005780:Gelatin; D002116:Calcitonin