Study on the partial purification of gonadotropins and thyrotropin from deer (Cervus elaphus) pituitaries.
Deer pituitaries were extracted with Tris-Cl buffer and the extract was fractionated on Concanavalin A-Sepharose into an unadsorbed ConA I fraction and an adsorbed ConA II fraction. The ConA II fraction was chromatographed on CM-cellulose to yield an unadsorbed CM I fraction and an adsorbed CM II fraction. The CM I and CM II fractions were then respectively subjected to ion exchange chromatography on DEAE-cellulose to give the resultant unadsorbed DEAE I and adsorbed DEAE II fractions. The ConA II CM II DEAE I fraction was the chromatographic fraction with the highest potency in stimulating testosterone production by isolated rat Leydig cells and the highest immunoreactivity in a rat luteinizing hormone (LH) radioimmunoassay (RIA). The ConA II CM II DEAE II fraction represented the fraction enriched in thyroid stimulating hormone (TSH). It exhibited cross reactivity in a rat TSH RIA and much lower LH bioactivity than the LH-enriched ConA II CM II DEAE I fraction. The ConA II CM I DEAE II fraction represented the follicle stimulating hormone (FSH)-enriched fraction. The ConA I fraction demonstrated negligible FSH immunoreactivity and LH bioactivity, indicating that most of deer FSH and LH were adsorbed on ConA-Sepharose.
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Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T.
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D000818:Animals; D001681:Biological Assay; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D003670:Deer / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification; D006065:Gonadotropins, Pituitary / Q000302:isolation & purification*; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000378:metabolism; D007986:Luteinizing Hormone / Q000302:isolation & purification / Q000502:physiology; D008297:Male; D010902:Pituitary Gland / Q000737:chemistry*; D011863:Radioimmunoassay; D051381:Rats; D013739:Testosterone / Q000096:biosynthesis; D013972:Thyrotropin / Q000302:isolation & purification*
D006065:Gonadotropins, Pituitary; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin