Characterization of a rice gene coding for a lipid transfer protein.
The cloning and sequence analysis of a gene that encodes a lipid transfer protein (LTP) from rice is reported. A genomic DNA library from Oryza sativa was screened using a cDNA encoding a maize LTP. One genomic clone containing the gene (Ltp) was partially sequenced and analyzed. The open reading frame is interrupted by an 89-bp intron. From the results of Southern hybridizations, Ltp appears to be a member of a small multigenic family. Transcripts of the corresponding gene were detected in several tissues including coleoptile, leaf, endosperm, scutellum and root. The transcription start point was determined by primer extension. The deduced amino-acid sequence of the Ltp product is shown to be homologous to LTPs from other crops.
Vignols
F
F
Laboratoire de Physiologie et Biologie Moléculaire Végétales, URA CNRS 565, Université de Perpignan, France.
Lund
G
G
Pammi
S
S
Trémousaygue
D
D
Grellet
F
F
Kader
J C
JC
Puigdomènech
P
P
Delseny
M
M
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001482:Base Composition; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D012275:Oryza / Q000737:chemistry / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C511920:lipid transfer proteins, plant