title: Insulin-like growth factor-I (IGF-I) responses in rats maintained with intravenous or intragastric infusion of total parenteral nutrition solutions containing medium- or long-chain triglyceride emulsions.
abstract: Our objective was to determine how intravenous or intragastric feeding affects serum concentrations of insulin-like growth factor (IGF)-I and -II and IGF binding proteins (IGFBP), and hepatic abundance of IGF-I mRNA. Male Fischer 344 rats (235-246 g) were fed for 14 d by intravenous or intragastric infusion with total parenteral nutrition (TPN) solutions providing 65% of energy from long-chain triglyceride (LCT) or a 3:1 admixture of medium-chain triglyceride (MCT) and LCT emulsions (MCT/LCT). Twice as much TPN solution was required per gram of weight gain with MCT/LCT compared with LCT infusion (P < 0.0003). Serum IGF-I and -II concentrations and hepatic IGF-I mRNA abundance were not significantly different. Circulating concentrations of IGFBPs with molecular weights of 38,000-43,000 (IGFBP-3) were significantly greater with intravenous MCT/LCT than with intravenous LCT infusion. Our data demonstrate that reduced growth in rats given TPN containing MCT/LCT compared with LCT emulsions is not associated with reduced serum IGF-I concentrations or hepatic abundance of IGF-I mRNA, although serum IGFBPs are elevated.
authors.affiliation: Department of Nutritional Sciences, University of Wisconsin-Madison 53706.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: D M
authors.initials: DM
authors.lastname: Ney
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000032:analysis; D004333:Drug Administration Routes; D005217:Fat Emulsions, Intravenous; D013006:Growth Hormone / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000302:isolation & purification / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D010289:Parenteral Nutrition, Total; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D014280:Triglycerides / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D005217:Fat Emulsions, Intravenous; D012333:RNA, Messenger; D014280:Triglycerides; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D013006:Growth Hormone; D004247:DNA