[The effect of inhibitors of DNA and protein synthesis on the chromosome aberration yield in a human lymphocyte culture exposed to gamma and neutron irradiation at different stages of the mitotic cycle. The cytogenetic effects in the G0 phase].
The effect of inhibitors of protein synthesis, cycloheximide (CH), and DNA synthesis, hydroxyurea (OU) in combination with 5-fluoro-2-deoxyuridine (OU + FDUR), on the yield of chromosome aberrations in cultured human lymphocytes induced by gamma- and neutron irradiation with doses of 1 and 2 Gy in G0 phase of mitotic cycle has been studied. The inhibitors added immediately after gamma-irradiation have caused reliable increase of aberration rate (CH at both doses and OU + FDUR at a dose of 2 Gy only). Both inhibitors caused no effect being added after 1.5, 3 and 5 hr after gamma-irradiation. The chromosome aberration rate substantially increased at both doses of neutron irradiation for both inhibitors added at all studied time intervals after exposure. Chromosome exchange aberrations have most contributed to the increase of aberration rate.
Golub
E V
EV
Bogatykh
B A
BA
Sevan'kaev
A V
AV
D001798:Blood Proteins / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002869:Chromosome Aberrations; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005467:Floxuridine / Q000494:pharmacology; D005720:Gamma Rays; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects*; D009502:Neutrons; D016192:Resting Phase, Cell Cycle / Q000187:drug effects / Q000528:radiation effects*; D013997:Time Factors
D001798:Blood Proteins; D005467:Floxuridine; D004247:DNA; D003513:Cycloheximide; D006918:Hydroxyurea
Vliianie ingibitorov sinteza DNK i belka na vykhod aberratsiĭ khromosom v kul'ture limfotsitov cheloveka pri gamma- i neĭtronnom obluchenii v razlichnykh stadiiakh mitoticheskogo tsikla. Tsitogeneticheskie éffekty v stadii G0.