[Effect of prolactin on production of acid phosphatase in rat prostatic cells and its mechanism].
The present study revealed the effect of prolactin (PRL) and sex steroid hormones on production of prostatic acid phosphatase (PAP) of the immature rat prostatic epithelial cells in serum-free medium. The results showed that PRL significantly stimulated synthesis and secretion of PAP, but failed to synergize with dihydrotestosterone or estradiol. Dihydrotestosterone or estradiol alone did not promote PAP production. Studies on the mechanism of PRL action indicated that adenyl cyclase was not activated in the action of PRL: Synergistic effect between PRL and prostaglandin E2 and F2 alpha was revealed in the stimulation of PAP production, and the effect of PRL could be blocked by indomethacin (a prostaglandin synthesis inhibitor). In conclusion, PRL directly stimulated PAP production of the cultured rat prostatic epithelial cells in serum-free medium and prostaglandin was involved in PRL action.
Zhang
C Q
CQ
State Key Laboratory of Reproductive Biology, Institute of Zoology, Academia Sinica, Beijing.
Zhuang
L Z
LZ
Yang
C R
CR
D000135:Acid Phosphatase / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology; D008297:Male; D011388:Prolactin / Q000494:pharmacology*; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004967:Estrogens; D011388:Prolactin; D000135:Acid Phosphatase