Deletion mutants of poly(ADP-ribose) polymerase support a model of cyclic association and dissociation of enzyme from DNA ends during DNA repair.
With an in vitro DNA repair system, Satoh and Lindah [(1992) Nature 356, 356-358] demonstrated that unmodified poly(ADP-ribose) polymerase (PADPRP) binds to radiation-damaged DNA and inhibits repair in the absence of NAD. However, in the presence of NAD, PADPRP undergoes automodification and the DNA is repaired. It was hypothesized that PADPRP cycles between an unmodified form, which protects DNA breaks, and an automodified form, which is released from DNA, thereby allowing access to repair enzymes. We have now tested this model with bacterially expressed mutants of PADPRP with deletions in the three major functional domains of the enzyme [Cherney, B. W., Chaudry, B., Bhatia, K., Butt, T. R., & Smulson, M. E. (1991) Biochemistry 30, 10420-10427]. Deletion mutants with an intact amino-terminal DNA-binding domain, and therefore capable of binding to DNA strand breaks in the in vitro assay, inhibited repair; however, whether the deletion was in the NAD-binding, active site domain or the automodification domain, the inhibition of repair exerted by these mutant proteins was not alleviated by NAD. A PADPRP mutant with a deletion in the DNA-binding domain did not inhibit DNA repair. Thus, the behavior of these PADPRP deletion mutants is consistent with the model proposed earlier. The model was also supported by experiments with Manley extracts of HeLa cells stably transfected with a PADPRP antisense RNA construct. Extracts of cells induced to express antisense RNA did not markedly inhibit in vitro DNA repair, nor did the addition of NAD influence the assay. In contrast, noninduced cell extracts inhibited repair and inhibition was alleviated by NAD.(ABSTRACT TRUNCATED AT 250 WORDS)
Smulson
M
M
Georgetown University School of Medicine, Department of Biochemistry and Molecular Biology, Washington, D.C. 20007.
Istock
N
N
Ding
R
R
Cherney
B
B
D001549:Benzamides / Q000494:pharmacology; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D004270:DNA, Circular / Q000378:metabolism; D017353:Gene Deletion; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D016296:Mutagenesis; D009243:NAD / Q000494:pharmacology; D010957:Plasmids; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D016372:RNA, Antisense / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D014162:Transfection
D001549:Benzamides; D004270:DNA, Circular; D016372:RNA, Antisense; D011994:Recombinant Proteins; D009243:NAD; C037689:benzamide; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases