Biochemical consequences of a large deletion in the mitochondrial genome of a Drosophila subobscura strain.
A mutant strain of D. Subobscura possesses two populations of mitochondrial genomes: a population identical to that of the wild strain (20%) and a dominant population (80%) which has lost more than 30% of its coding zone by deletion. Spectrophotometric determination of respiratory complex activities shows that: complex I (5 genes implicated in deletion) presents maximal activity reduced by 40%, whereas that of complex III (concerned by cytochrome b) is lowered by 30%. Nevertheless, polarographic determinations of substrate oxidation show activity of complex I to be reduced by 30%. In contrast, complex III activity is similar to that measured in the wild strain. The predominant use of one part of the respiratory chain may account for the fact that the mutant strain is apparently unaffected by mutation.
Debise
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Laboratoire de Biochimie, URA-CNRS 360, Université Blaise Pascal, Clermont II, Aubière, France.
Touraille
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Durand
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Alziari
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D000818:Animals; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000502:physiology*; D004330:Drosophila / Q000235:genetics*; D014450:Electron Transport Complex III / Q000235:genetics / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D017344:Genes, Insect; D005971:Glutamates / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000235:genetics*; D012402:Rotenone / Q000494:pharmacology; D017384:Sequence Deletion; D013386:Succinates / Q000378:metabolism
D004272:DNA, Mitochondrial; D005971:Glutamates; D005994:Glycerophosphates; D008293:Malates; D013386:Succinates; D012402:Rotenone; C029620:alpha-glycerophosphoric acid; D016660:NAD(P)H Dehydrogenase (Quinone); D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III