To the mechanism of spermine-FBS cytotoxicity toward K562 human myelogenous leukemia cells.
The effects of several compounds acting through adenylate cyclase system and/or influencing prostaglandin biosynthesis on spermine-FBS cytotoxicity to human myelogenous leukemia K562 cells were studied. Salbutamol, a beta 2-adrenoceptor agonist inhibited to a certain extent spermine-FBS cytotoxic action to K562 cells, and propranolol, a beta 2-adrenoceptor antagonist, did not affect this inhibition. Aminophylline, an inhibitor of cyclic nucleotide phosphodiesterase, acted suppressing spermine-FBS cytotoxicity to K562 cells. Pretreatment of the cells with dexamethasone did not significantly alter salbutamol-related inhibition of spermine-FBS cytotoxicity. Indomethacin, an inhibitor of cyclooxygenases directly involved in prostaglandin biosynthesis, did not interfere with protective terbutaline effects against spermine-FBS cytotoxicity to K562 cells during the 24-hour period.
Juranić
Z
Z
Institute of Oncology and Radiology, Belgrade, Yugoslavia.
Joksimović
J
J
Spuzić
I
I
Sami
I
I
Juranić
I
I
D000420:Albuterol / Q000494:pharmacology; D000628:Aminophylline / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D005312:Fetal Blood / Q000502:physiology; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology*; D011433:Propranolol / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000628:Aminophylline; D013096:Spermine; D003907:Dexamethasone; D011433:Propranolol; D000420:Albuterol