title: Free triiodothyronine (FT3) EIA-BioMerieux. Analytical and clinical evaluation.
abstract: We tested a new immuno-enzymatic kit: FT3-EIA BioMerieux, for direct measurement of FT3. Precision is good, with coefficients of variation below 10%. Detection limit is 0.4 pmol/l. We tested some anticoagulants and the specificity of the antibody. Results appear to be independent of plasmatic concentration of albumin and TBG. Reference range was determined to be 3.7 to 8.7 pmol/l. A clinical study, in comparison with isotopic method FT3-Coat-RIA BioMerieux shows that this non-isotopic kit is available, even for low concentrations.
authors.affiliation: Laboratoire de Biochimie, Centre Hospitalier, Saint-Germain-en-Laye, France.
authors.forename: M J
authors.initials: MJ
authors.lastname: Bugugnani
authors.forename: G
authors.initials: G
authors.lastname: Corbet
authors.forename: B
authors.initials: B
authors.lastname: Fleury
authors.forename: C
authors.initials: C
authors.lastname: Aubry
mesh_terms: D000418:Albumins / Q000032:analysis; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood*; D007037:Hypothyroidism / Q000097:blood*; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D012016:Reference Values; D013959:Thyroid Diseases / Q000097:blood*; D013975:Thyroxine-Binding Proteins / Q000032:analysis; D014284:Triiodothyronine / Q000032:analysis*
chemical_list: D000418:Albumins; D013975:Thyroxine-Binding Proteins; D014284:Triiodothyronine