The promoter of the Xwnt-5C gene contains octamer and AP-2 motifs functional in Xenopus embryos.
The Xwnt-5C gene is expressed in Xenopus embryos from the early gastrula stage onwards. The transcription of Xwnt-5C is regulated differentially with respect to transcript size, timing and localization. To gain insight into the generation of the Xwnt-5C expression pattern, we started to analyze the transcriptional regulation of this gene. We isolated Xwnt-5C genomic DNA sequences. By microinjection of chimaeric reporter constructs into Xenopus embryos we demonstrate that the upstream region contains a promoter functional in vivo. Of the several putative binding sites for trans-acting factors, present in a minimal promoter fragment, some have been studied in more detail. Mutations in an octamer motif and in an AP-2 consensus sequence interfere with the activity of the Xwnt-5C minimal promoter. In vitro binding assays with extracts from gastrula stage Xenopus embryos show that the octamer motif of the Xwnt-5C promoter can bind several Octamer binding factors, one of which is Oct1.
Kuiken
G A
GA
Hubrecht Laboratory, Utrecht, The Netherlands.
Bertens
P J
PJ
Peterson-Maduro
J
J
Veenstra
G J
GJ
Koster
J G
JG
Destrée
O H
OH
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D046508:Culture Techniques; D004247:DNA; D004268:DNA-Binding Proteins; D008845:Microinjections; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D014981:Xenopus; D029867:Xenopus Proteins
D004268:DNA-Binding Proteins; D011506:Proteins; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D029867:Xenopus Proteins; C493675:tfap2a protein, Xenopus; D004247:DNA