Binding of carbohydrates to solid supports: evaluation of a prototype system.
Mono- and disaccharides were covalently and irreversibly bound to aminopolystyrene beads in good yield by heating in dilute aqueous solution. The degree and stability of sugar binding were determined by chemical and radiochemical methods and the accessibility of the bound sugars was demonstrated by exoglycosidase hydrolysis and by an enzyme-linked lectin-binding assay using Concanavalin A.
Tweeddale
H J
HJ
School of Chemistry, Macquarie University, North Ryde, NSW, Australia.
Batley
M
M
Mei
X G
XG
Redmond
J W
JW
D002845:Chromatography / Q000379:methods; D005947:Glucose / Q000031:analogs & derivatives*; D008320:Maltose / Q000031:analogs & derivatives*; D010084:Oxidation-Reduction; D011137:Polystyrenes / Q000737:chemistry*
D011137:Polystyrenes; C014329:aminopolystyrene; D008320:Maltose; D005947:Glucose