title: A determination of the solution conformation of the nonmammalian tachykinin eledoisin by NMR and CD spectroscopy.
abstract: The nonmammalian tachykinin eledoisin was investigated by use of CD and two-dimensional NMR techniques. In aqueous solution the peptide is conformationally averaged, but on addition of 50% trifluoroethanol (TFE) or sodium dodecyl sulfate (SDS) it adopts an alpha-helical structure. In TFE/H2O and SDS, residues 6-10 of eledoisin show more conformational order than the terminal regions, which undergo dynamic fraying. A possible turn in the N-terminal "address" region, the putative receptor recognition site of the peptide, is detected by NMR spectroscopy but appears to undergo substantial conformational averaging. The NMR data indicate that the helical central core of eledoisin is better defined in the micellar environment than in TFE; however, partial unfolding via 3(10) intermediates occurs in both cases. The conformational preference for SDS-bound eledoisin was examined by three-dimensional structure calculations using NMR-derived distance information in simulated annealing calculations.
authors.affiliation: Victorian College of Pharmacy, Monash University, Parkville, Australia.
authors.forename: J C
authors.initials: JC
authors.lastname: Wilson
authors.forename: K J
authors.initials: KJ
authors.lastname: Nielsen
authors.forename: M J
authors.initials: MJ
authors.lastname: McLeish
authors.forename: D J
authors.initials: DJ
authors.lastname: Craik
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004600:Eledoisin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D012967:Sodium Dodecyl Sulfate; D012996:Solutions; D012997:Solvents; D015320:Tachykinins / Q000737:chemistry; D014270:Trifluoroethanol; D014867:Water
chemical_list: D008823:Micelles; D012996:Solutions; D012997:Solvents; D015320:Tachykinins; D014867:Water; D012967:Sodium Dodecyl Sulfate; D004600:Eledoisin; D014270:Trifluoroethanol