Tissue and serum levels of steroid hormones and RU 486 after administration of mifepristone.
The present study was designed to examine the effect RU 486 administration on steroid hormone levels in serum and decidua. Sixty women at 6-7 weeks gestation were divided into three groups. The first group took a placebo 24 hours before interruption of pregnancy. The other two groups took 200 mg mifepristone 12 and 24 hours before the surgical procedure, respectively. The concentrations of steroids and mifepristone were measured by radioimmunoassay or high performance liquid chromatography. Mifepristone treatment increased the levels of estradiol, cortisol and testosterone in serum and decidual cytosol (p < 0.05 or p < 0.01). A minor elevation in progesterone level was observed but was not statistically significant. The tissue levels of progesterone, estradiol and testosterone were much higher than the serum levels, whereas RU 486 concentration in the tissue was only one-third of the serum level. In addition, the RU 486 level in decidual cytosol was of the same order as that of progesterone. This competitive concentration was not achieved in chorionic villi in our previous observation, explaining why mifepristone exerts its predominant effect on decidua rather than villi. It is concluded that RU 486 reached an effective inhibitory concentration in decidua and had significant effects on the endocrine milieu.
Wang
J D
JD
Department of Cell Biology, National Research Institute for Family Planning, Beijing, People's Republic of China.
Shi
W L
WL
Zhang
G Q
GQ
Bai
X M
XM
D000028:Abortion, Induced; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D003656:Decidua / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism*; D015735:Mifepristone / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood / Q000378:metabolism*; D011863:Radioimmunoassay; D013739:Testosterone / Q000097:blood / Q000378:metabolism*
D015735:Mifepristone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D006854:Hydrocortisone