The influence of 25-hydroxycholesterol on phosphatidylinositol content in cultured smooth muscle cells.
The effect of 25-hydroxycholesterol on phosphatidylinositol content was studied in subconfluent human arterial smooth muscle cells exposed to myo-[2-3H]inositol for 90 min and then to 25-hydroxycholesterol at different concentrations or for different incubating periods. Ethanol at a concentration of 0.05% in culture medium was used as control. The results demonstrate that an increased incubation time or an increase in the concentration of 25-hydroxycholesterol produced an increase in phosphatidylinositol and its degraded products. The ratio of precursor to product at each stage of phosphatidylinositol turnover in 25-hydroxycholesterol-treated cells, however, was not different from that in control cells. The data indicate that the presence of 25-hydroxycholesterol in the media induced general activation of phosphatidylinositol synthesis and turnover.
Zhou
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Burnsides Research Laboratory, University of Illinois, Urbana 61801.
Kummerow
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FA
D002478:Cells, Cultured; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*
D006888:Hydroxycholesterols; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; C007997:25-hydroxycholesterol