title: Establishing a resuscitation model in rabbits with closed-thoracic cardiopulmonary by-pass.
abstract: This study aims to establish an animal model of resuscitation in rabbits by using closed-thoracic cardiopulmonary by-pass (CTCPB). The rabbits were randomly divided into four groups according to cardiac arrest times which were 8, 10, 12, and 15 min. Neurologic outcome and blood lactate were determined within 150 min after resuscitation. It was indicated that the rabbits' neurologic functions were progressively injured with prolonged cardiac arrest time. The threshold of circulatory arrest time that induced a vegetative state in the rabbits was between 10 and 12 min. There were no significant differences in cardiac resuscitability among the four groups, as was so for plasma lactate, although it increased significantly from the control levels. The establishment of a small-animal model of resuscitation by using CTCPB, and the problems in dealing with it are also described and discussed in detail in this paper. Our experience indicated that this is a simple, convenient, and economical animal model for the study of resuscitation.
authors.affiliation: Laboratory of Anesthesiology, First Hospital, Beijing Medical University, China.
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: Q
authors.initials: Q
authors.lastname: Ma
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.forename: D
authors.initials: D
authors.lastname: Ma
mesh_terms: D000818:Animals; D002315:Cardiopulmonary Bypass / Q000379:methods*; D004195:Disease Models, Animal; D004569:Electroencephalography; D005260:Female; D006323:Heart Arrest / Q000601:surgery*; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008297:Male; D011817:Rabbits; D012151:Resuscitation; D016896:Treatment Outcome
chemical_list: D007773:Lactates; D019344:Lactic Acid