Rat hypothalamic NPY mRNA and brown fat uncoupling protein mRNA after high-carbohydrate or high-fat diets.
We measured the influence of diet composition on hypothalamic neuropeptide Y (NPY) message and brown fat uncoupling protein (UCP) mRNA using different diets. Sprague-Dawley rats ate ad libitum either chow, a high-carbohydrate (HC), an intermediate-carbohydrate (IHC), a high-fat (HF), or an intermediate-fat (IHF) diet, all with equal protein content (g/kcal). The HF and IHF groups ate less food mass and, except for HC, all groups consumed similar kilocalories during the study. After 1 wk, we killed the animals and extracted total RNA from arcuate nucleus, cortex, and brown adipose tissue (BAT). Arcuate NPY mRNA in the HF group was significantly (P < 0.001) lower than in the HC and chow group. There were no differences between groups in NPY message in cortex or NPY protein in the paraventricular nucleus. BAT UCP message levels were significantly higher (P = 0.001) in the HF group. Thus HF compared with HC and chow diet reduces expression of NPY mRNA in hypothalamic nuclei and increases expression of BAT UCP message.
Giraudo
S Q
SQ
Neuroendocrine Research Laboratory, Department of Veterans Affairs Medical Center, Minneapolis, Minnesota 55417.
Kotz
C M
CM
Grace
M K
MK
Levine
A S
AS
Billington
C J
CJ
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D004041:Dietary Fats; D004044:Dietary Proteins; D002149:Energy Intake; D015870:Gene Expression; D007031:Hypothalamus / Q000378:metabolism*; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D009478:Neuropeptide Y / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D000071256:Uncoupling Protein 1; D015430:Weight Gain
D002352:Carrier Proteins; D004041:Dietary Fats; D004044:Dietary Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D009478:Neuropeptide Y; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1