title: Effect of ethanol on paraquat toxicity in F344 rats.
abstract: The potential modifying effects of ethanol and paraquat on lesion development in livers and lungs of male F344 rats were studied. Animals were divided into diethylnitrosamine (DEN)-initiated and non-initiated groups, subgroups of each being exposed to 10 ml 20% ethanol/kg body weight, 2.5 or 10 mg paraquat/kg body weight or a combination of 10 ml 20% ethanol/kg body weight and 2.5 or 10 mg paraquat/kg body weight, given by intragastric intubation three times a week. Controls received 10 ml saline/kg body weight. All animals were subjected to two-thirds partial hepatectomy at the end of wk 3 and killed at the end of wk 8. All five rats receiving 10 mg paraquat/kg body weight without DEN-pretreatment died before termination of the experiment, but the additional ethanol treatment saved animals: only one of five rats died. Rats administered 2.5 or 10 mg paraquat/kg body weight demonstrated lung toxicity, as evidenced by fibrosis and hyperplasia, but not when simultaneously treated with ethanol in DEN-pretreated groups. In the liver, however, evaluation of glutathione S-transferase placental form (GST-P)-positive foci did not reveal any influence of the treatments on lesion development in DEN-initiated animals. It is concluded that ethanol decreases paraquat toxicity, and that neither agent, alone or in combination, exerts any hepatocarcinogenic modification potential.
authors.affiliation: First Department of Pathology, Nagoya City University Medical School, Japan.
authors.forename: P
authors.initials: P
authors.lastname: Puapairoj
authors.forename: L
authors.initials: L
authors.lastname: Cui
authors.forename: K
authors.initials: K
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Akagi
authors.forename: R
authors.initials: R
authors.lastname: Hasegawa
authors.forename: N
authors.initials: N
authors.lastname: Ito
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury; D004052:Diethylnitrosamine / Q000494:pharmacology; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D006965:Hyperplasia; D007674:Kidney Diseases / Q000139:chemically induced; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced; D008168:Lung / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced; D008297:Male; D010269:Paraquat / Q000008:administration & dosage / Q000633:toxicity*; D010920:Placenta / Q000201:enzymology; D011658:Pulmonary Fibrosis / Q000139:chemically induced; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D004052:Diethylnitrosamine; D000431:Ethanol; D005982:Glutathione Transferase; D010269:Paraquat