Specific region of the c-myc promoter is responsive to electric and magnetic fields.
The level of c-myc transcripts is increased in cells exposed to extremely low frequency (elf) electromagnetic (EM) fields at 60 Hz. The aim of the present experiments was to determine if regulatory regions upstream of the c-myc gene modulate the response to EM fields. DNA upstream of P1 of both mouse and human c-myc genes was transfected into cells as CAT constructs. The presence of DNA 5' to the human or mouse myc genes results in increased expression of CAT following 20 min exposures of cells to 60 Hz elf EM fields. Specific portions of the human upstream DNA were deleted and introduced into cells. The region responsive to EM fields is located between -353 and -1,257 relative to the P1 promoter.
Lin
H
H
Department of Pathology, Columbia University Health Sciences, New York, New York 10032.
Goodman
R
R
Shirley-Henderson
A
A
D000818:Animals; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000528:radiation effects*; D016259:Genes, myc / Q000528:radiation effects*; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D051379:Mice; D009101:Multiple Myeloma; D011401:Promoter Regions, Genetic / Q000528:radiation effects*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
D016271:Proto-Oncogene Proteins c-myc; D011993:Recombinant Fusion Proteins