H-2-specific T suppressor cells. I. Evidence for T suppressor inducer and effector molecules in suppression.
The induction of H-2-specific Ts cells was accomplished by the i.v. injection of X-irradiated (2000R) C57BL/6 spleen cells into CBA/J mice. These Ts cells significantly (78%) suppressed the delayed type hypersensitivity (DTH) response in CBA/J mice injected s.c. with X-irradiated (2000R) C57BL/6 spleen cells and given a footpad challenge with the same cell population 5 days later. We have shown that both CD4 and CD8 T cells were involved in the observed suppression, and these cells secrete T suppressor inducer (Tsfi) and T suppressor effector (Tsfe) molecules. Both Tsfi and Tsfe molecules were shown to significantly inhibit (> 87%) one-way mixed lymphocyte responses between CBA/J and mitomycin C-treated C57BL/6 spleen cells. Using an adoptive transfer method, we showed that mice given both a primary and secondary immunization with X-irradiated C57BL/6 spleen cells to induce H-2-specific Ts cells contain a significantly greater number of Ts cells than mice given only a primary immunization, suggesting the presence of memory Ts cells.
Malley
A
A
Oregon Regional Primate Research Center, Beaverton 97006-3499.
Zeleny-Pooley
M
M
Kelleher-Mayo
S
S
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006183:H-2 Antigens / Q000276:immunology*; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D016219:Immunotherapy, Adoptive; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology / Q000528:radiation effects; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000528:radiation effects; D014184:Transplantation, Homologous / Q000276:immunology*
D006183:H-2 Antigens