The effect of indomethacin and reserpine on renal vascular escape.
Isolated rabbit kidneys were perfused with Krebs-Henseleit solution in an open system designed to allow study of dose-response relationships. The effects of the cyclooxygenase inhibitor indomethacin and of reserpine on (norepinephrine) noradrenaline (NOR)-induced renal vascular escape were studied. Indomethacin (50 mg/Kg) was given two hours prior to surgery followed by continuous infusion (0.33 mg/min), and blocked renal vascular escape in all doses of NOR tested (10(-7)-10(-4) M) by 70-80%. Reserpine (10 mg/Kg, given 10-12 hours prior to organ perfusion), also suppressed renal vascular escape significantly by 80-90%. We conclude that there is a participation of prostaglandins in noradrenaline induced escape in the perfused rabbit kidney.
Fonteles
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MC
Departamento de Fisiologia e Farmacologia, Centro de Ciencias da Saude, Ceara, Brazil.
de Lima e Forti
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CA
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D066298:In Vitro Techniques; D007213:Indomethacin / Q000008:administration & dosage / Q000494:pharmacology*; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D011817:Rabbits; D012110:Reserpine / Q000008:administration & dosage / Q000494:pharmacology*; D014655:Vascular Resistance / Q000187:drug effects*
D012110:Reserpine; D009638:Norepinephrine; D007213:Indomethacin