Effect of hyperlipacidemia on plasma C-peptide concentration during euglycemic hyperinsulinemic clamp.
During constant infusion of exogenous human insulin and glucose to healthy volunteers, a rise in glycemia was still able to stimulate the B-cell as judged from the increase in C-peptide plasma concentration. Nevertheless, under euglycemic conditions, the C-peptide concentration fell well below its initial level. This coincided, however, with severe hypolipacidemia. When the latter phenomenon was corrected through the simultaneous administration of exogenous triglycerides and heparin, a reascension in C-peptide plasma concentration was observed despite persistent hyperinsulinemia. It is proposed, therefore, that the hyperinsulinemic-euglycemic clamp procedure does not represent a reliable approach to explore the possible feedback action of insulin upon its own secretion rate, because of the interference upon B-cell secretory activity of factors such as changes in lipacidemia.
Conget
I
I
Endocrinology and Diabetes Unit, Hospital Clinic, Barcelona, Spain.
Gonzalez-Clemente
J M
JM
Casals
E
E
Casamitjana
R
R
Gomis
R
R
Malaisse
W J
WJ
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002096:C-Peptide / Q000097:blood*; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D015309:Glucose Clamp Technique; D006801:Humans; D007262:Infusions, Intravenous; D007328:Insulin / Q000008:administration & dosage / Q000097:blood* / Q000494:pharmacology*; D008297:Male; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012016:Reference Values; D013997:Time Factors; D014280:Triglycerides / Q000097:blood*
D001786:Blood Glucose; D002096:C-Peptide; D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D011994:Recombinant Proteins; D014280:Triglycerides