Human alpha S1-casein like protein: purification and N-terminal sequence determination.
Casein components of human milk are generally reported to belong to the beta- and kappa-groups. In this research human casein fraction was obtained from pooled mature milk, either by ultracentrifugation or by acid precipitation. In both cases a minor component with a slightly higher mobility in SDS-PAGE than beta-casein was identified. The protein was purified to homogeneity, the N-terminal sequence of the first 14 amino acid residues of this new human casein subunit shows a high degree of homology with the alpha s1-casein sequences from other species.
Cavaletto
M
M
Dipartimento Biologia Animale, Università di Torino, Italy.
Cantisani
A
A
Giuffrida
G
G
Napolitano
L
L
Conti
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D002364:Caseins