Non-classical receptors for aldosterone in plasma membranes from pig kidneys.
Rapid, nongenomic in vitro effects of aldosterone on intracellular electrolytes, cell volume and the sodium-proton antiporter have been found in human mononuclear leukocytes (HML), as have related membrane receptors. In the present study, binding of 125I-labeled aldosterone to plasma membrane preparations from pig kidneys was studied, since nongenomic in vitro effects of aldosterone have also been described in cultured kidney cells. In this preparation, binding of aldosterone shares important features with both functional and binding data in HML. These include a very low apparent Ki of approximately 0.1 nM for aldosterone, a high turnover rate and binding selectivity for aldosterone and fludrocortisone. Desoxycorticosterone acetate and corticosterone show intermediate affinity, with apparent Ki values of approximately 1 and 100 nM, with hydrocortisone even less active. Thus binding of aldosterone to kidney plasma membranes is compatible with the major features of its nongenomic renal effects.
Christ
M
M
Medizinische Klinik, Klinikum Innenstadt, University of Munich, Germany.
Sippel
K
K
Eisen
C
C
Wehling
M
M
D000450:Aldosterone / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D003345:Corticosterone / Q000378:metabolism; D003900:Desoxycorticosterone / Q000378:metabolism; D005438:Fludrocortisone / Q000378:metabolism; D006632:Histamine / Q000031:analogs & derivatives / Q000378:metabolism; D007457:Iodine Radioisotopes; D007668:Kidney / Q000378:metabolism*; D018161:Receptors, Mineralocorticoid / Q000378:metabolism*; D013552:Swine
D007457:Iodine Radioisotopes; D018161:Receptors, Mineralocorticoid; D003900:Desoxycorticosterone; D000450:Aldosterone; D006632:Histamine; C078230:aldosterone-3-(O-carboxymethyl)oximino-(2-iodohistamine); D005438:Fludrocortisone; D003345:Corticosterone