Generation of allo-reactive cytotoxic T lymphocytes by particle bombardment-mediated gene transfer.
Mature T lymphocytes comprise functionally distinct subsets with discrete roles in the regulation of the immune response. The cellular basis of the anti-tumor effect is now understood to involve the activation and expansion of tumor-specific cytotoxic T lymphocytes (CTL). To immuno-potentiate the generation of CTL, we have employed the biolistic system for the genetic immunization of mice. Here, we report the efficient generation of anti-H-2Kb allo-reactive CTL by particle acceleration-mediated genetic immunization of mouse spleen cells with H-2Kb DNA. The insertion and expression of exogenous gene into host spleen cells following in situ genetic inoculation to effect the generation of a cellular immune response may permit novel alternative strategies for immunotherapy.
Hui
K M
KM
Laboratory of Molecular Immunology, National University of Singapore.
Sabapathy
T K
TK
Oei
A A
AA
Chia
T F
TF
D000818:Animals; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D007114:Immunization; D008722:Methods; D051379:Mice; D008806:Mice, Inbred AKR; D010957:Plasmids / Q000235:genetics; D013154:Spleen / Q000276:immunology / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000502:physiology*; D013997:Time Factors
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D004247:DNA