Isolation and properties of the subunit form EF-1C of elongation factor 1 from Guerin epithelioma cells.
EF-1C is a component of the aggregate EF-1B, consisting of the subunit forms EF-1A.EF-1C; it was isolated by dissociation of this aggregate in the presence of GTP. The subunit form EF-1C stimulates binding of aminoacyl-tRNA to ribosomes, catalysed by EF-1A, similarly as EF-1 beta gamma which stimulates the activity of EF-1 in other eukaryotic cells. EF-1C in the presence of 6 M urea was separated into two polypeptides. Polypeptide of molecular mass 32,000 Da is responsible for regeneration of the EF-1A.GTP active complex. Thermal sensitivity of EF-1A was much higher than that of EF-1B, thus a protective role of EF-1C in the EF-1A.EF-1C complex is suggested.
Marcinkiewicz
C
C
Department of General and Organic Chemistry, Medical Academy, Białystok, Poland.
Gałasiński
W
W
D000818:Animals; D002277:Carcinoma / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D046911:Macromolecular Substances; D009363:Neoplasm Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000737:chemistry*; D010452:Peptide Biosynthesis; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D010455:Peptides; D012360:RNA, Transfer, Phe / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D046911:Macromolecular Substances; D009363:Neoplasm Proteins; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010455:Peptides; D012360:RNA, Transfer, Phe; D006153:Guanosine Diphosphate; C012001:polyphenylalanine; D006160:Guanosine Triphosphate