title: Gelatinase activity of exoantigens from virulent and non-virulent isolates of Paracoccidioides brasiliensis.
abstract: Differences in the occurrence of components with gelatinase activity were detected among four isolates of Paracoccidioides brasiliensis: Pb339 and Pb18 (highly virulent), and Pb265 and Pb18AV (very low virulence). Culture filtrates from these isolates were electrophoresed in substrate gels and tested for gelatinase activity. Pb339 showed three enzyme bands of apparent molecular masses: 43, 53 and 78 kDa; Pb18 had two bands, one at 59 kDa and another with molecular mass higher than 78 kDa. Isolate Pb18AV showed only one band at 78 kDa and Pb265 exhibited a component of molecular mass which failed to enter the separating gel.
authors.affiliation: Department of Immunology, Universidade de São Paulo, Brasil.
authors.forename: C A
authors.initials: CA
authors.lastname: Vaz
authors.forename: D W
authors.initials: DW
authors.lastname: Mackenzie
authors.forename: V
authors.initials: V
authors.lastname: Hearn
authors.forename: Z P
authors.initials: ZP
authors.lastname: Camargo
authors.forename: L M
authors.initials: LM
authors.lastname: Singer-Vermes
authors.forename: E
authors.initials: E
authors.lastname: Burger
authors.forename: V L
authors.initials: VL
authors.lastname: Calich
mesh_terms: D000946:Antigens, Fungal; D018093:Gelatinases / Q000737:chemistry / Q000378:metabolism*; D010228:Paracoccidioides / Q000201:enzymology* / Q000472:pathogenicity*; D013045:Species Specificity; D014774:Virulence
chemical_list: D000946:Antigens, Fungal; D018093:Gelatinases