A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium.
In this study, two transformation vectors (pMG101 and pMG103) for Phanerochaete chrysosporium were constructed, based on the ble phleomycin-resistance-encoding gene and a homologous histone H4 promoter. Transformation frequencies were 6-10 per micrograms of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of ble-dependent resistance. Plasmid pMG103, which lacks the H3, does not show methylation.
Gessner
M
M
Universität Kaiserslautern, Germany.
Raeder
U
U
D001426:Bacterial Proteins / Q000235:genetics; D001487:Basidiomycota / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D004271:DNA, Fungal / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors; D006657:Histones / Q000235:genetics*; D008745:Methylation; D010692:Phleomycins / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004271:DNA, Fungal; D005819:Genetic Markers; D006657:Histones; D010692:Phleomycins