title: The analysis of rate limitation within enzymes: relations between flux control coefficients of rate constants and unidirectional rates, rate constants and thermodynamic parameters of single isolated enzymes.
abstract: The extent to which a rate constant or step within an enzyme mechanism limits the net enzyme rate in a particular condition can be quantified as a flux control coefficient. We derive here a number of relations between the control coefficients and the unidirectional rates, rate constants, and thermodynamic parameters of the enzyme. These and other relations are used to suggest a number of methods for experimentally measuring control coefficients within enzymes.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College, London, U.K.
authors.forename: G C
authors.initials: GC
authors.lastname: Brown
authors.forename: C E
authors.initials: CE
authors.lastname: Cooper
mesh_terms: D004798:Enzymes / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D013816:Thermodynamics
chemical_list: D004798:Enzymes