Suppression of murine IgE responses with ovalbumin-pullulan conjugates: comparison of the suppressive effect of different conjugation methods and different molecular weights of pullulan.
Ovalbumin (OVA)-pullulan conjugates were made using four different conjugation methods and eight different molecular weights of pullulan ranging from 4,200 to 600,000. Pretreatment of mice by the administration of conjugates made by using cyanulic chloride or cyanogen bromide and pullulan of molecular weight 40,000 or more, anti-OVA IgE antibody response was suppressed completely and anti-OVA IgM and IgG antibody response was enhanced. In contrast, by the administration of conjugates made by using an oxidation or thiol activation method, only partial suppression of anti-OVA IgE antibody response was achieved and no enhancement of anti-OVA IgM and IgG antibody production was observed.
Uchida
T
T
Department of Safety Research on Biologics, National Institute of Health, Tokyo, Japan.
Ikegami
H
H
Ando
S
S
Kurimoto
M
M
Mitsuhashi
M
M
Naito
S
S
Usui
M
M
Matuhasi
T
T
D000818:Animals; D005936:Glucans / Q000737:chemistry / Q000276:immunology*; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis; D051379:Mice; D008970:Molecular Weight; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D013329:Structure-Activity Relationship
D005936:Glucans; D007073:Immunoglobulin E; C009109:pullulan; D010047:Ovalbumin