Enzyme-linked immunosorbent assay-enzyme-linked coagulation assay for detection of antibodies to Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes.
The solution-phase complex assay for toxins A, B, and E from Clostridium botulinum (Elcatech, Inc., Winston-Salem, N.C.) was modified to measure antibody. The addition of unlabeled polyclonal antibodies to a mixture consisting of toxin with chicken antibody and RVV-XA-labeled horse antibody reduces the sensitivity of detection of neurotoxin. This reduction in sensitivity can be used as a measure of the specific antibody titer.
Doellgast
G J
GJ
Department of Biochemistry, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27157.
Triscott
M X
MX
Beard
G A
GA
Bottoms
J D
JD
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D001667:Binding, Competitive; D001681:Biological Assay; D001905:Botulinum Toxins / Q000276:immunology*; D002645:Chickens; D003014:Clostridium botulinum / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006736:Horses; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D001905:Botulinum Toxins