title: Biological and immunological activities of bovine placental gonadotropin.
abstract: A bovine placental extract was fractionated on a Concanavalin A (ConA)-Sepharose column into an unadsorbed ConA I fraction and an adsorbed ConA II fraction. The ConA II fraction was then separated by gel filtration on Sephadex G-100 into void volume material (ConA II Sephadex fraction I) and retarded material (ConA II Sephadex fraction II). Con A II Sephadex fraction II was considerably more potent than ConA II Sephadex fraction I in stimulating testosterone production by isolated rat Leydig cells. Con A I fraction was inactive in this regard. None of the aforementioned fractions manifested follicle stimulating hormone (FSH) bioactivity. ConA II Sephadex fraction II yielded a displacement curve parallel to the standard curve in radioimmunoassays for rat luteinizing hormone and thyroid stimulating hormone (TSH), but it did not cross-react in a radioimmunoassay for rat FSH. ConA II fraction did not exhibit FSH bioactivity. The results indicate that bovine placental gonadotropin possesses luteinizing hormone-like bioactivity and immunoreactivity and TSH immunoreactivity but neither FSH bioactivity nor immunoreactivity.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories.
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000818:Animals; D002417:Cattle; D006063:Chorionic Gonadotropin / Q000502:physiology; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D006062:Gonadotropins / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000378:metabolism; D007986:Luteinizing Hormone / Q000502:physiology; D008297:Male; D010920:Placenta / Q000737:chemistry*; D011863:Radioimmunoassay; D051381:Rats; D013739:Testosterone / Q000096:biosynthesis; D013972:Thyrotropin / Q000502:physiology
chemical_list: D006063:Chorionic Gonadotropin; D006062:Gonadotropins; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin