Genetic typing of the DQA1*4 alleles by restriction enzyme digestion of the PCR product obtained with the DQ Alpha Amplitype kit.
The three DQ Alpha 4 alleles, 4.1 (0501), 4.2 (0401), and 4.3 (0601) cannot be distinguished with the reverse dot blot DQ Alpha Amplitype Kit. A previous report (Yunis, I. et al., Tissue Antigens, Vol. 39, 1992, pp. 182-186) indicated that the typing of these three alleles can be accomplished by endonuclease digestion of the PCR product that is produced following amplification with the DQ Alpha primers with Fok I and Rsa I. We report here the use of this method to type the DQA1*0401, 0501 and 0601 alleles in the PCR product obtained with the DQ Alpha-Amplitype Kit. We have analyzed the PCR product obtained in over 200 forensic samples. We have found that in all of these cases, it is possible to assign a type to those samples that type as DQ Alpha 4 with the Amplitype Kit. Furthermore, we have found the technique to be useful in some cases where it has not been previously possible to distinguish individuals or samples that have the DQ Alpha 4 allele and type identically with respect to all other DQ Alpha alleles.
Salazar
M
M
Dana-Farber Cancer Institute, Boston, MA.
Williamson
J
J
Bing
D H
DH
D000483:Alleles; D001483:Base Sequence; D004247:DNA; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine / Q000379:methods*; D005787:Gene Frequency; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D044469:Racial Groups / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D015183:Restriction Mapping
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D011933:Reagent Kits, Diagnostic; D004247:DNA