Overexpression of pancreatic secretory trypsin inhibitor in pancreatic cancer. Evaluation of its biological function as a growth factor.
Four clones of pancreatic secretory trypsin inhibitor (PSTI)-overexpressing cells (TF-PANC clones 1, 6, 8, and 36) were established to evaluate the physiological function of PSTI secreted by cancer cells, by means of introducing a PSTI-expression vector (pRSV-PSTI) into the human pancreatic adenocarcinoma cell line (PANC-1). No obvious changes were observed in the histological features of these transplanted tumors in nude mice, in the growth of TF-PANC and PANC-1, or that of 3T3 fibroblasts when cocultured with them. Addition of recombinant human PSTI to the cultured media resulted in no increase in proliferation of fibroblasts (3T3 and WI-38) or of four pancreatic cancer cell lines (PANC-1, CAPAN-I, MIAPaCa-2, and Hs766T). These results suggest that the estimation of tumor-bearing PSTI as a paracrine or autocrine growth factor in recent studies should be given careful consideration.
Ohmachi
Y
Y
Department of Surgery II, Osaka University Medical School, Japan.
Murata
A
A
Matsuura
N
N
Yasuda
T
T
Uda
K
K
Mori
T
T
D016475:3T3 Cells; D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011994:Recombinant Proteins / Q000494:pharmacology; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000235:genetics* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D011994:Recombinant Proteins; D014359:Trypsin Inhibitor, Kazal Pancreatic