The receptor-recycling and lysosome biogenesis mutant TfT1.11 belongs to a new complementation group, End6.
We have previously isolated a Chinese hamster ovary (CHO) mutant with a temperature-dependent pleiotropic defect in receptor recycling. This mutant, TfT1.11, has also been shown to have defects in fluid-phase endocytosis and lysosome biogenesis. Previously isolated CHO cell mutants with defects in endocytosis have been assigned to five recessive complementation groups (End1-End5). We have performed complementation analysis using polyethylene glycol-induced fusion of genetically marked sublines of TfT1.11 with representative mutants from each of the End groups. Complementation of the receptor trapping and lysosome biogenesis defects as well as temperature lethality was observed with all groups, demonstrating that TfT1.11 defines a new complementation group, End6.
Bucci
M
M
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.
Moyer
T W
TW
Brown
C M
CM
Wilson
R B
RB
Murphy
R F
RF
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004705:Endocytosis / Q000235:genetics; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D006822:Hybrid Cells; D008247:Lysosomes / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism*
D011990:Receptors, Transferrin