Isolation, characterization and sequence of a cDNA encoding gamma-tubulin protein from the fern Anemia phyllitidis L. Sw.
A complete cDNA encoding gamma-tubulin protein from Anemia phyllitidis is presented. The deduced amino acid sequence shows an average similarity of 81-86% to known gamma-tubulin genes from insects, mammals and fungi. Northern blots indicate the existence of transcripts of ca. 1.9 kb which are expressed during gametogenesis of A. phyllitidis. The gene described is the first cloned and entirely sequenced gamma-tubulin gene in any plant.
Fuchs
U
U
Abteilung Allgemeine Botanik, Universität Ulm, Germany.
Moepps
B
B
Maucher
H P
HP
Schraudolf
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014404:Tubulin / Q000235:genetics*
D018076:DNA, Complementary; D014404:Tubulin