title: The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames.
abstract: We report the sequence of an 18,002 bp DNA fragment from the right arm of Saccharomyces cerevisiae chromosome XI. This segment contains nine complete open reading frames (ORFs), YKR401 to YKR409, and part of another ORF, YKR400, covering altogether 87.2% of the entire sequence. One of them, YKR400, encodes an NAD-dependent 5,10-methylene-tetrahydrofolate dehydrogenase. YKR404, YKR405 and YKR406 correspond to the previously characterized HBS1, MRP-L20 and PRP16 genes, coding for a translation elongation factor, a mitochondrial ribosomal protein and an ATP-binding protein, respectively. The putative product of YKR407 contains the zinc-binding region signature of neutral zinc metallopeptidases. The five other ORFs do not show significant homology to any known protein.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: García-Cantalejo
authors.forename: V
authors.initials: V
authors.lastname: Baladrón
authors.forename: P F
authors.initials: PF
authors.lastname: Esteban
authors.forename: M A
authors.initials: MA
authors.lastname: Santos
authors.forename: G
authors.initials: G
authors.lastname: Bou
authors.forename: M A
authors.initials: MA
authors.lastname: Remacha
authors.forename: J L
authors.initials: JL
authors.lastname: Revuelta
authors.forename: J P
authors.initials: JP
authors.lastname: Ballesta
authors.forename: A
authors.initials: A
authors.lastname: Jiménez
authors.forename: F
authors.initials: F
authors.lastname: del Rey
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D000619:Aminohydrolases / Q000235:genetics; D001483:Base Sequence; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000235:genetics; D019204:GTP-Binding Proteins; D005800:Genes, Fungal / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins; D008667:Metalloproteins / Q000235:genetics; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics; D008928:Mitochondria; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics*; D010445:Peptide Elongation Factors / Q000235:genetics; D020365:RNA Helicases; D000072260:RNA Splicing Factors; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015032:Zinc
chemical_list: D029968:Escherichia coli Proteins; D005656:Fungal Proteins; C077247:HBS1 protein, S cerevisiae; D018840:HSP70 Heat-Shock Proteins; C488993:MRPL20 protein, S cerevisiae; D008667:Metalloproteins; D024101:Mitochondrial Proteins; D009097:Multienzyme Complexes; D010445:Peptide Elongation Factors; D000072260:RNA Splicing Factors; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C055342:methylene tetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase; C488992:rplT protein, E coli; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D010450:Endopeptidases; D000619:Aminohydrolases; D000251:Adenosine Triphosphatases; D019204:GTP-Binding Proteins; C075669:PRP16 protein, S cerevisiae; D020365:RNA Helicases; D015032:Zinc