Neuroendocrine phenotype differentiation in a hamster lung epithelial cell line under low oxygen pressure or after transformation by diethylnitrosamine.
Oxygen pressure as low as 5% in the gas phase or 87 mmHg in the liquid phase induced various neuroendocrine cell (NEC) phenotypes in more than 80% of cells of a cloned fetal Syrian hamster lung epithelial cell line (M3E3/C3). Further, cells from a number of colonies transformed in an anchorage-independent manner by diethylnitrosamine (DEN) demonstrated a NEC phenotype. Since the cell line used is of a pluripotent stem cell type, both hypoxia and DEN appear to possess a potency for NEC phenotype induction.
Emura
M
M
Institut für Experimentelle Pathologie, Hochschule Hannover, Germany.
Ochiai
A
A
Gobert-Bohlen
A
A
Panning
B
B
Dungworth
D L
DL
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D006224:Cricetinae; D003470:Culture Media; D004052:Diethylnitrosamine / Q000633:toxicity*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005260:Female; D008168:Lung / Q000166:cytology* / Q000187:drug effects*; D008647:Mesocricetus; D009490:Neurosecretory Systems / Q000166:cytology* / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D010641:Phenotype; D011312:Pressure; D012701:Serotonin / Q000493:pharmacokinetics
D003470:Culture Media; D012701:Serotonin; D004052:Diethylnitrosamine; D010100:Oxygen