Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements.
In transgenic tobacco plants the regulatory region of the Agrobacterium genes involved in mannopine synthesis (pmas) directs expression in vascular tissues and is induced upon wounding. To identify cis-acting sequences required for the cell-type-specific and wound-inducible expression of this regulatory region, the expression pattern of chimeric genes in which 5' upstream deletions of the pmas 1' promoter direct the expression of the beta-glucuronidase coding sequence, was analyzed in transgenic tobacco plants. It was found that the pmas1' promoter is specifically expressed in phloem cells and that this tissue-specific pattern of expression is the result of the interaction of at least two negative regulatory elements with a positive element that directs expression in many cell types. It was also found that wound induction is mediated by two different mechanisms, one of them involving a dramatic change in tissue-specific expression.
Guevara-García
A
A
Departamento de Ingeniería Genética de Plantas, Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato, Guanajuato, México.
Mosqueda-Cano
G
G
Argüello-Astorga
G
G
Simpson
J
J
Herrera-Estrella
L
L
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000472:pathogenicity; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D017343:Genes, Plant; D005809:Genes, Regulator; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D014018:Tissue Distribution; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology
D009687:Nuclear Proteins; D010940:Plant Proteins; D004247:DNA; D006836:Hydro-Lyases; C070395:mannopine synthase