Differences in the effects of two hexachlorobiphenyls on superoxide generation by polymorphonuclear leucocytes stimulated by N-formyl-methionyl-leucyl-phenylalanine and phorbol myristate acetate.
The effects of hexachlorobiphenyls (HCBs) on superoxide (O2-) generation by guinea pig polymorphonuclear leucocytes were examined. 2,3,6,2',3',6'-HCB by itself had only a weak inductive effect on O2- generation. This compound, however, enhanced O2- generation stimulated by N-formyl-methionyl-leucyl-phenylalanine (FMLP) about twofold, but not the generation induced by phorbol myristate acetate (PMA). On the other hand, 3,4,5,3',4',5'-HCB suppressed O2- generation stimulated by both FMLP and PMA. The inhibitory potency of this compound was far greater with PMA (ID50, 5 microM) than with FMLP (ID50, 40 microM).
Iwata
M
M
Department of Pharmacology, Okayama University Medical School, Japan.
Nishihara
Y
Y
Watanabe
Y
Y
Miyahara
M
M
Saeki
K
K
D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D003574:Cytochrome c Group; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011078:Polychlorinated Biphenyls; D013482:Superoxide Dismutase; C010498:2,3,6,2',3',6'-hexachlorobiphenyl; D013755:Tetradecanoylphorbol Acetate; C027309:3,4,5,3',4',5'-hexachlorobiphenyl