Immunohistochemical detection of alkaline phosphatase in formalin-fixed and paraffin-embedded rat organs by means of avidin-biotin peroxidase complex method.
The author attempted the detection of alkaline phosphatase (ALP) protein in various rat organs, which were fixed with formalin and embedded in paraffin, by means of the avidin-biotin peroxidase complex (ABC) method. Reaction products were detected after a usual incubation time of 30 min at room temperature in anti-ALP antibody. Positive reactions were observed in the kidney and liver but not in the small intestine, and this indicates that the antibody used in the present study reacts only with the tissue-nonspecific type of ALP.
Chida
K
K
Department of Pathology, Kitasato University School of Hygienic Sciences, Kanagawa, Japan.
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D001360:Avidin; D001710:Biotin; D005260:Female; D005557:Formaldehyde; D007150:Immunohistochemistry / Q000379:methods*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D010179:Pancreas / Q000201:enzymology; D016612:Paraffin Embedding; D051381:Rats; D017208:Rats, Wistar; D016707:Tissue Fixation
D001360:Avidin; D005557:Formaldehyde; D001710:Biotin; D000469:Alkaline Phosphatase