title: Comparison of bovine immune responses to affinity-purified bovine herpesvirus-1 antiidiotypes and glycoproteins.
abstract: Bovine immune responses to rabbit antiidiotypic antibodies (anti-Id) against neutralizing monoclonal antibodies to bovine herpesvirus-1 (BHV-1) envelope glycoproteins and to BHV-1 glycoproteins were compared. Glycoprotein-immunized animals produced high titers of anti-BHV-1 antibodies and were protected against BHV-1 challenge. Recombinant bovine interleukin-2 (rIL-2)-treated, anti-Id-immunized animals showed a slight reduction in clinical disease, and one calf produced BHV-1-neutralizing antibodies. Treatment with rIL-2 augmented non-BHV-1-specific immune responses. However, even with rIL-2 as an adjuvant, the mixture of polyclonal anti-Id did not elicit a consistent, protective BHV-1-specific immune response in calves.
authors.affiliation: Department of Pathology and Microbiology, Kansas State University, Manhattan.
authors.forename: D J
authors.initials: DJ
authors.lastname: Orten
authors.forename: W
authors.initials: W
authors.lastname: Xue
authors.forename: S
authors.initials: S
authors.lastname: van Drunen Littel-van den hurk
authors.forename: O Y
authors.initials: OY
authors.lastname: Abdelmagid
authors.forename: D N
authors.initials: DN
authors.lastname: Reddy
authors.forename: M
authors.initials: M
authors.lastname: Campos
authors.forename: L A
authors.initials: LA
authors.lastname: Babiuk
authors.forename: F
authors.initials: F
authors.lastname: Blecha
authors.forename: H C
authors.initials: HC
authors.lastname: Minocha
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002417:Cattle; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007111:Immunity, Cellular; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007376:Interleukin-2; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins