title: A novel 34 kDa glutathione-binding protein in mature rat ovary.
abstract: A novel protein that binds to a glutathione-Sepharose affinity column has been detected in mature, but not immature, rat ovary. This protein could be resolved from all identifiable components when the affinity-purified material, containing primarily glutathione transferases, was analyzed on reversed phase-HPLC. The unidentified protein migrated with an apparent molecular weight of 34 kDa on SDS-PAGE. After purification by affinity chromatography and subsequent preparative electrophoresis, the protein was subjected to N-terminal amino acid sequence analysis. The sequence obtained demonstrated a high degree of homology with an internal amino acid sequence in human carbonyl reductase (EC 1.1.1.184).
authors.affiliation: Department of Biochemistry, Wallenberg Laboratory, Stockholm University, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Toft
authors.forename: M
authors.initials: M
authors.lastname: Söderström
authors.forename: M B
authors.initials: MB
authors.lastname: Ahlberg
authors.forename: J W
authors.initials: JW
authors.lastname: DePierre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000032:analysis* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010053:Ovary / Q000737:chemistry* / Q000201:enzymology; D011485:Protein Binding; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D005982:Glutathione Transferase; D005978:Glutathione