Comparison and genomic sizing of Escherichia coli O157:H7 isolates by pulsed-field gel electrophoresis.
Genomic DNAs of Escherichia coli O157:H7 strains isolated from patients and food samples were analyzed by pulsed-field gel electrophoresis. The rare-cutting endonucleases SfiI and XbaI generated 6 and 10 distinct genomic profiles, respectively, for the 22 strains analyzed, indicating that this technique may find application for epidemiologic studies. Summation of XbaI fragments from five E. coli O157:H7 strains estimated the genomic length at ca. 4.7 Mb.
Harsono
K D
KD
Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
Kaspar
C W
CW
Luchansky
J B
JB
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005516:Food Microbiology; D016680:Genome, Bacterial; D006801:Humans; D008970:Molecular Weight; D013045:Species Specificity
D004269:DNA, Bacterial; C041749:endodeoxyribonuclease SfiI; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific