CTL responses to the gag polyprotein encoded by the murine AIDS defective retrovirus are strain dependent.
Murine AIDS (MAIDS) is induced by a mixture of retroviruses, of which a replication defective virus is the proximal agent of disease. This defective virus harbors a single intact gene that encodes an aberrant gag polyprotein. Certain mouse strains are genetically resistant to MAIDS, with several genes, including H-2Dd, contributing to this resistance. Because MHC class I gene products present intracellular Ags to CTL, recombinant viruses were used to determine whether gag-specific CTLs mediate the genetic linkage between H-2Dd and resistance. Interestingly, while genetically resistant BALB/cByJ and C57BL/KsJ mice (H-2d) generated gag-specific CD8+ CTLs, a similar response was not detected in susceptible BALB.B and C57BL/6J mice (H-2b). However, this CTL response does not appear to be responsible for genetic resistance because: 1) a vigorous CTL response could also be generated by susceptible (C57BL/6 x BALB/cBy) F1 mice and 2) the relevant epitope is H-2Kd restricted.
Schwarz
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Department of Microbiology, Dartmouth Medical School, Lebanon, NH 03756.
Green
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D000818:Animals; D001483:Base Sequence; D003602:Cytotoxicity, Immunologic; D017931:DNA Primers / Q000737:chemistry; D003673:Defective Viruses; D015683:Gene Products, gag / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D008810:Mice, Inbred C57BL / Q000276:immunology*; D008969:Molecular Sequence Data; D016183:Murine Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000382:microbiology; D012190:Retroviridae / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D017931:DNA Primers; D015683:Gene Products, gag; D006183:H-2 Antigens