Expression of env gene of bovine leukemia virus in rodent cells.
The BamHI-BamHI fragment of the env gene of bovine leukemia virus (BLV) cloned in pMMEx expression vector was transfected into Chinese hamster cells. Monoclonal antibodies (MAbs) directed against both conformational and sequential epitopes of gp51 of BLV recognized viral polypeptides expressed in hamster cells in Western blotting and enzyme-linked immunosorbent assay.
Zajac
V
V
Department of Experimental Pathology, Slovak Academy of Sciences, Bratislava.
Slavikova
K
K
Babusikova
O
O
D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015686:Gene Products, env / Q000032:analysis / Q000096:biosynthesis*; D015752:Genes, env; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000378:metabolism*; D008168:Lung; D015183:Restriction Mapping; D014162:Transfection
D004279:DNA, Viral; D015686:Gene Products, env