Cholinergic activation of phosphoinositide metabolism during soman-induced seizures.
In the present study we investigated the role of the cholinergic pathway in phosphoinositide metabolism activation observed during soman-induced convulsions. We thus studied the effect of atropine sulphate, a muscarinic antagonist (20 mg kg-1, i.p.), on IP3 levels in rat hippocampus. We demonstrated that initially, the increase of IP3 is closely seizure-related. On the other hand, after 10 min of seizures, the IP3 enhancement and the seizure activity are no longer correlated. After 20 min of seizures, atropine failed to inhibit soman-induced IP3 enhancement, suggesting that the activation of another neurotransmitter system(s) linked to PPI turnover succeeds the cholinergic stimulation.
Bodjarian
N
N
Unité de Neurotoxicologie, Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Carpentier
P
P
Blanchet
G
G
Baubichon
D
D
Lallement
G
G
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D017776:Autonomic Pathways / Q000187:drug effects; D001711:Biotransformation / Q000502:physiology; D004569:Electroencephalography / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007293:Inosine Triphosphate / Q000378:metabolism; D008297:Male; D010275:Parasympathetic Nervous System / Q000502:physiology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D012999:Soman
D010716:Phosphatidylinositols; D007293:Inosine Triphosphate; D001285:Atropine; D012999:Soman