Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal.
The influence of PGF2 alpha on c-jun gene expression in ovary, uterus and adrenal was examined. Three and seven day postovulatory PMSG primed immature rats received 500 ug PGF2 alpha by two subcutaneous injections 8 hours apart. Control rats received saline. Animals were sacrificed 30 minutes after the second injection of PGF2 alpha. Tissues were obtained and frozen in liquid nitrogen. RNA extracted from ovary, uterus and adrenal was analyzed by Northern and slot blot. c-jun was expressed differentially in these organs. An increase in c-jun expression by PGF2 alpha treatment occurred in the ovary but not in the adrenal and uterus. The effect of PGF2 alpha on c-jun was stronger in older compared to younger corpora lutea. These results indicate differential regulation of c-jun by PGF2 alpha in steroidogenic and steroid responsive tissues and that c-jun might be linked to the mode of action of PGF2 alpha in luteolysis.
Khan
I
I
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
Hossain
A
A
Whitman
G F
GF
Sarkar
N H
NH
McDonough
P G
PG
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D015237:Dinoprost / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D016758:Genes, jun; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000378:metabolism*
D012333:RNA, Messenger; D015237:Dinoprost