Actions of interleukin-4 on prostaglandin biosynthesis by human amnion cells.
The effects of interleukin-4 (IL-4) on amnion prostaglandin (PG) production have been determined. Human amnion cells from term placentae were grown to confluence and incubated with IL-4 either alone or with various concentrations of epidermal growth factor (EGF) of ionomycin. PGE2 production was determined using a specific radioimmunoassay. IL-4 alone stimulated PGE2 production in amnion cells in a concentration-related manner. IL-4 significantly enhanced the stimulatory actions of ionomycin and EGF on amnion cell PGE2 production. Ionomycin and IL-4 acted synergistically in their effects on PGE2 production by amnion cells whereas EGF and IL-4 acted more additively in this respect. We suggest that IL-4 production by immune effector cells in gestational tissues may contribute to the mechanisms of labor at term and preterm.
Adamson
S
S
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
Edwin
S S
SS
LaMarche
S
S
Mitchell
M D
MD
D000650:Amnion / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000494:pharmacology* / Q000502:physiology; D015759:Ionomycin / Q000494:pharmacology; D007743:Labor, Obstetric / Q000502:physiology; D011247:Pregnancy; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D015847:Interleukin-4; D015759:Ionomycin; D004815:Epidermal Growth Factor; D015232:Dinoprostone