title: Cloning and characterization of the Inc A/C plasmid RA1 replicon.
abstract: The Inc A/C plasmids, like Inc P and Inc Q plasmids, have a broad host range. However, their maintenance functions remain to be studied. An autoreplicative region of 2.79 kb named RepA/C, able to replicate both in the family Enterobacteriaceae and in Pseudomonas spp., was isolated and sequenced. The stability, copy number, and incompatibility expression of this replicon were determined. RepA/C and a nonautoreplicative fragment of 16 kb of this replicon were used as probes and showed specific hybridizations with the Inc P3-A/C plasmids from Pseudomonas spp. and members of the Enterobacteriaceae. These probes could be used as tools for identification of the plasmids of this epidemiologically important Inc group.
authors.affiliation: Laboratoire de Bactériologie, Université de Franche-Comté, Besançon, France.
authors.forename: C
authors.initials: C
authors.lastname: Llanes
authors.forename: P
authors.initials: P
authors.lastname: Gabant
authors.forename: M
authors.initials: M
authors.lastname: Couturier
authors.forename: Y
authors.initials: Y
authors.lastname: Michel-Briand
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004261:DNA Replication; D004755:Enterobacteriaceae / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000145:classification / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics*; D012093:Replicon / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D015342:DNA Probes