Immunolocalization of PKC zeta in rat photoreceptor inner segments.
We have utilized several peptide specific antisera directed against the C-terminals (Wetsel et al, 1992) of several protein kinase C (PKC) isozymes (alpha, beta 1, beta 11, gamma, delta, epsilon, zeta) to delineate the cellular localization of these PKC isozymes in rat retina. Antisera against PKC beta 1, beta 11, gamma, delta and epsilon were non-reactive in frozen rat retina sections, whereas, anti PKC alpha was strongly reactive with the outer plexiform, inner plexiform and nerve fiber cell layers. The most specific localization of immunoreactivity was observed with PKC zeta, which reacted strongly and exclusively with photoreceptor inner segments, but not outer segments. Immunoblot analysis of whole rat retina homogenate showed that anti-PKC alpha recognized an antigen of approximately 80kD and anti-PKC zeta recognized a approximately 72kD protein. Immunolocalization of PKC zeta to photoreceptor inner segments and possible functional significance are discussed.
Ghalayini
A J
AJ
Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030.
Koutz
C A
CA
Wetsel
W C
WC
Hannun
Y A
YA
Anderson
R E
RE
D000595:Amino Acid Sequence; D000818:Animals; D003623:Dark Adaptation; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000138:chemical synthesis / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism; D010786:Photoreceptor Cells / Q000201:enzymology*; D011493:Protein Kinase C / Q000138:chemical synthesis / Q000378:metabolism*; D051381:Rats
D007527:Isoenzymes; D010455:Peptides; D011493:Protein Kinase C