High performance liquid chromatography of penicillins with penicillin-enhanced luminol chemiluminescence detection.
A high performance liquid chromatographic method with chemiluminescence detection for the determination of penicillin G and ampicillin is reported. The method is based on the enhancement of the luminol chemiluminescence with beta-lactam antibiotics. The linear relationship was obtained between the peak height and the concentration of penicillin G or ampicillin up to 15 nmol per 20 microL injection. Detection limits were 1 nmol for penicillin G and 0.5 nmol for ampicillin with a signal-to-noise ratio of 2. Relative standard deviations for five replicate measurements of 4 nmol/injection each of penicillin G and ampicillin were 2.1 and 2.3%, respectively.
Nakashima
K
K
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Kawaguchi
S
S
Akiyama
S
S
Schulman
S G
SG
D000667:Ampicillin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003035:Cobalt; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D008165:Luminol; D010400:Penicillin G / Q000032:analysis; D010406:Penicillins / Q000032:analysis*; D012972:Sodium Hydroxide
D010406:Penicillins; D003035:Cobalt; D012972:Sodium Hydroxide; D008165:Luminol; D000667:Ampicillin; D010400:Penicillin G