title: CT-boxes are involved in control of the rat insulin II gene expression.
abstract: Expression of the rat insulin II gene is controlled mainly at the level of transcription initiation by multiple factors binding to specific cis-acting DNA-elements in the regulatory region. We have shown that two elements (CT-motifs) located between nucleotides -83 and -76 (CT-1) and -204 and -197 (CT-2) are involved in transcriptional regulation in the insulin-producing cell line HIT M2.2.2. Transient expression analysis of 5'-deletion as well as block replacement mutants revealed that CT-1 and CT-2 are mutational sensitive. Gel mobility shift assays showed that both motifs bind similar nuclear factors. Our results suggest the involvement of a third CT-motif located directly upstream of CT-2 on the complementary strand.
authors.affiliation: Institut für Biochemie, Medizinische Fakultät, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Rommel
authors.forename: I B
authors.initials: IB
authors.lastname: Leibiger
authors.forename: B
authors.initials: B
authors.lastname: Leibiger
authors.forename: R
authors.initials: R
authors.lastname: Walther
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase; D006224:Cricetinae; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D007328:Insulin / Q000235:genetics* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017384:Sequence Deletion; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D007328:Insulin; D015500:Chloramphenicol O-Acetyltransferase