title: Determination of octadecadienoic acids in human serum: a critical appraisal.
abstract: The method based on high-performance liquid chromatography currently used to determine the lipid levels of octadeca-9(Z),11(E)-dienoic acid in human serum fails to distinguish between this acid and octadeca-10(E),12(Z)-dienoic acid. Adducts with the fluorescent dienophile 4-[3-(1-propenyl)propyl]-1,2,4-triazoline-3,5-dione permit the separation of these two isomeric acids as does gas-liquid chromatography of their methyl esters, but these alternatives produce equivocal results when applied to biological samples.
authors.affiliation: Department of Chemistry, University of Exeter, UK.
authors.forename: G
authors.initials: G
authors.lastname: Read
authors.forename: N R
authors.initials: NR
authors.lastname: Richardson
authors.forename: D G
authors.initials: DG
authors.lastname: Wickens
mesh_terms: D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005231:Fatty Acids, Unsaturated; C027369:octadecadienoic acid