A point mutation in the chloroplast rps12 gene from Nicotiana plumbaginifolia confers streptomycin resistance.
In an effort to understand the mechanism of streptomycin resistance in Nicotiana plumbaginifolia, we have sequenced the chloroplast rps12 gene, a potential molecular target. We report that a streptomycin-resistant mutant isolated from protoplast cultures of N. plumbaginifolia contains an A-to-G transition at nucleotide position 149 in exon 2 of the chloroplast rps12 gene. The detected point mutation predicts a substitution of arginine for lysine in a phylogenetically conserved region.
Hsu
C M
CM
Institute of Life Science, National Tsing Hua University, Hsinchu, Taiwan, ROC.
Yang
W P
WP
Chen
C C
CC
Lai
Y K
YK
Lin
T Y
TY
D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism*; D004247:DNA; D004351:Drug Resistance / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010947:Plants, Toxic; D017354:Point Mutation; D012269:Ribosomal Proteins / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*
D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D004247:DNA; D013307:Streptomycin