Studies of non-covalent interactions of actinomycin D with single-stranded oligodeoxynucleotides by ion spray mass spectrometry and tandem mass spectrometry.
Non-covalent binding of actinomycin D (AMD) to single-stranded DNA (ssDNA) was observed by ion spray mass spectrometry. Interactions between AMD and different sequences of non-self-complementary oligodeoxynucleotides were investigated as a function of pH. Non-covalent AMD/ssDNA complexes disappeared at low pH, and no ssDNA complexes were detected with rifampicin (RP). Moreover, the different binding affinities between AMD and ssDNA with and without 5'G-C3' or 5'C-G3' base sequences were also demonstrated using ion spray mass spectrometry. Additional support for the non-covalent nature of binding in AMD/ssDNA complexes was obtained from tandem mass spectrometry.
Hsieh
Y L
YL
Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca 14850.
Li
Y T
YT
Henion
J D
JD
Ganem
B
B
D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D003609:Dactinomycin / Q000737:chemistry*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D012293:Rifampin / Q000494:pharmacology
D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D003609:Dactinomycin; D012293:Rifampin