A dot immunobinding assay in comparison with the gel diffusion test for the detection of equine herpesvirus-1 antigen from field samples.
The authors describe a rapid and simple dot immunobinding assay (DIA) for detection and identification of equine herpesvirus-1 antigen in field samples from cases of abortion, stillbirth, perinatal foal mortality and paralysis. The assay employs a nitrocellulose membrane to which antigen is adsorbed as a dot. Antigen is identified as a coloured dot using a procedure based on the principle of enzyme-linked immunosorbent assay (ELISA). In all, 61 samples were tested by DIA and the test was compared with conventional agar gel immunodiffusion (AGID). With DIA, 44 (72%) samples gave positive results, while only 22 of 61 (36%) samples tested positive by AGID. DIA was observed to be rapid, more sensitive and more specific than AGID, in addition to the obvious advantage of being reagent-conservative, inexpensive and simple to perform.
Richa
Department of Veterinary Microbiology, Haryana Agricultural University, Hisar, India.
Grover
Y P
YP
Charan
S
S
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D005260:Female; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology / Q000302:isolation & purification*; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D015151:Immunoblotting / Q000662:veterinary*; D005779:Immunodiffusion / Q000662:veterinary*; D011247:Pregnancy
D000956:Antigens, Viral