Combined lectin binding and PAS/alcian blue staining in glycol methacrylate sections.
Evaluation of cryofixation and paraffin and glycol methacrylate embedding showed that lectin binding was essentially independent of the embedding medium. Fluorescence intensity increased in the following order: glycol methacrylate, paraffin and cryostat sections. The optical resolution increased in the reverse order. Semi-thin glycol methacrylate sections provided satisfactory fluorescence intensities and the best resolution of all embedding techniques applied. Furthermore the lectin treated sections can be stained further using routine histological or specific histochemical methods. The potassium hydroxide/alcian blue/periodic acid-phenylhydrazine-Schiff method was used successfully to demonstrate sulfated and nonsulfated sialomucins. Lectins combined with mucin histochemistry allowed visualization of specific sugar residues in the same glycol methacrylate plastic section.
Uhr
G
G
Institute of Zoology, Veterinary School of Hanover, Germany.
Giebel
J
J
Schwenk
M
M
D000423:Alcian Blue; D010131:Aminosalicylic Acid; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005453:Fluorescence; D006168:Guinea Pigs; D006651:Histocytochemistry; D037102:Lectins; D008689:Methacrylates; D016612:Paraffin Embedding / Q000379:methods*; D016613:Plastic Embedding / Q000379:methods*; D013270:Stomach / Q000033:anatomy & histology; D013552:Swine
D037102:Lectins; D008689:Methacrylates; D010131:Aminosalicylic Acid; C005044:hydroxyethyl methacrylate; D000423:Alcian Blue