Detection of the expression of the Epstein-Barr virus latent gene product in Hodgkin's lymph nodes by immunostaining. Comparison between paraffin sections and imprints.
The latent membrane protein (LMP) of Epstein-Barr virus can be detected by an immunostaining method in about 50% of Hodgkin's disease lymph node tumour cells on frozen sections. The same method had been successfully employed on paraffin-embedded (PE) sections. We used this immunostaining method on imprints (IP) and PE sections of 26 Hodgkin's lymph nodes. Overall, there were 11 LMP-positive cases; immunostaining was stronger in IP than in PE samples; in nine cases both IP and PE samples were LMP-positive; in two, imprints were LMP-positive when PE sections were LMP-negative.
Belkaïd
M I
MI
Service d'hématologie, Centre P et M Curie, Alger, Algeria.
Brière
J
J
Colonna
P
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002648:Child; D005260:Female; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008198:Lymph Nodes / Q000737:chemistry* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014763:Viral Matrix Proteins / Q000235:genetics / Q000302:isolation & purification*
D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins