Similar toxic effect of 1,3-bis(2-chloroethyl)-1-nitrosourea on lymphocytes from human subjects differing in the expression of glutathione transferase M1-1.
Sixteen healthy donors were investigated for the presence or absence of glutathione transferase (GST) M1-1 in lymphocytes by immunodetection with polyclonal antibodies against human GST M1-1. Nine out of 16 individuals (56%) were categorized as GST M1-1 positive. Phytohaemagglutinin stimulated lymphocytes from GST M1-1 positive and negative donors were treated with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and compared regarding inhibition of [3H]thymidine incorporation as a measure of cytotoxicity. No significant differences in the effect of BCNU were observed between the two groups, indicating that GST M1-1 is not an important resistance factor for BCNU.
Jungnelius
U
U
Department of Oncology, Karolinska Hospital, Stockholm, Sweden.
Ridderström
M
M
Hansson
J
J
Ringborg
U
U
Mannervik
B
B
D002330:Carmustine / Q000633:toxicity*; D015870:Gene Expression; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D010835:Phytohemagglutinins; D013936:Thymidine / Q000378:metabolism
D010835:Phytohemagglutinins; D005982:Glutathione Transferase; D002330:Carmustine; D013936:Thymidine