title: An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence.
abstract: Regions extremely variable in size and sequence occur at conserved locations in eukaryotic rRNAs. The functional importance of one such region was determined by gene reconstruction and replacement in Tetrahymena thermophila. Deletion of the D8 region of the large-subunit rRNA inactivates T. thermophila rRNA genes (rDNA): transformants containing only this type of rDNA are unable to grow. Replacement with an unrelated sequence of similar size or a variable region from a different position in the rRNA also inactivated the rDNA. Mutant rRNAs resulting from such constructs were present only in precursor forms, suggesting that these rRNAs are deficient in either processing or stabilization of the mature form. Replacement with D8 regions from three other organisms restored function, even though the sequences are very different. Thus, these D8 regions share an essential functional feature that is not reflected in their primary sequences. Similar tertiary structures may be the quality these sequences share that allows them to function interchangeably.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
authors.forename: R
authors.initials: R
authors.lastname: Sweeney
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: M C
authors.initials: MC
authors.lastname: Yao
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D017173:Caenorhabditis elegans / Q000235:genetics; D017124:Conserved Sequence; D003433:Crosses, Genetic; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics*; D004023:Dictyostelium / Q000235:genetics; D014644:Genetic Variation; D008961:Models, Structural; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017384:Sequence Deletion; D016808:Tetrahymena thermophila / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal