Induction of apoptosis in a T lymphoblastoid cell line infected with feline immunodeficiency virus.
The mechanism of cell death induced by feline immunodeficiency virus (FIV) infection was investigated in an interleukin 2(IL-2)-dependent T-lymphoblastoid cell line (MYA-1). DNA extracted from FIV-infected MYA-1 cells showed a ladder of nucleosomal DNA, indicating that the cytopathic effect (CPE) observed in these cells was due to apoptosis. Infection of MYA-1 cells with FIV was associated with suppression of the proliferative response of the cells to exogenous IL-2 prior to DNA fragmentation. These findings suggest that FIV-induced CPE in these T-lymphoblastoid cells is associated with apoptosis possibly due to a defect in the IL-2 signal transduction pathway.
Ohno
K
K
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Okamoto
Y
Y
Miyazawa
T
T
Mikami
T
T
Watari
T
T
Goitsuka
R
R
Tsujimoto
H
H
Hasegawa
A
A
D000818:Animals; D017209:Apoptosis; D002415:Cats; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004249:DNA Damage; D005434:Flow Cytometry; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000472:pathogenicity / Q000502:physiology*; D007376:Interleukin-2 / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology* / Q000382:microbiology
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins