Oligosaccharide sequences attached to an inert support (SYNSORB) as potential therapy for antibiotic-associated diarrhea and pseudomembranous colitis.
Toxin A produced by Clostridium difficile, the causative agent of pseudomembranous colitis and antibiotic-associated diarrhea, was shown to bind to synthetic oligosaccharide sequences attached to an inert support (SYNSORB). The oligosaccharide sequences that bind to toxin A were related to sequences previously identified as potential receptors for the toxin. Various SYNSORBs containing a variety of oligosaccharides were examined for their potential to neutralize toxin A activity from toxin-containing solutions as well as clinical stool samples from patients with either pseudomembranous colitis or antibiotic-associated diarrhea. The results from neutralization experiments suggest SYNSORB can effectively neutralize toxin A activity from stool samples and thus could serve as a potential therapy for C. difficile-associated diarrhea.
Heerze
L D
LD
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Kelm
M A
MA
Talbot
J A
JA
Armstrong
G D
GD
D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects; D001427:Bacterial Toxins; D016466:CHO Cells; D002240:Carbohydrate Sequence; D016360:Clostridioides difficile; D006224:Cricetinae; D003967:Diarrhea / Q000139:chemically induced / Q000382:microbiology / Q000628:therapy*; D004761:Enterocolitis, Pseudomembranous / Q000628:therapy*; D004768:Enterotoxins / Q000037:antagonists & inhibitors*; D005243:Feces; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000494:pharmacology* / Q000627:therapeutic use; D011817:Rabbits; D012822:Silicon Dioxide
D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D004768:Enterotoxins; D009844:Oligosaccharides; C041348:tcdA protein, Clostridium difficile; D012822:Silicon Dioxide