title: Prohibitin expression during cellular senescence of human diploid fibroblasts.
abstract: Prohibitin is an evolutionarily conserved gene postulated to possess tumor suppressor activity and to contribute to the limited lifespan of human diploid fibroblast-like cells. Prohibitin mRNA and protein expression and its ability to become post-translationally modified were determined in human diploid fibroblast-like cells of different in vitro ages. The expression of prohibitin mRNA and protein changes little with increasing in vitro age; however, its protein product is post-synthetically modified in younger but not older cells. These results suggest that prohibitin is similar to the retinoblastoma gene product whose anti-proliferative activity remains active in older cells because it is not post-synthetically modified.
authors.affiliation: Noble Center for Biomedical Research, Oklahoma Medical Research Foundation, Oklahoma City 73104.
authors.forename: X T
authors.initials: XT
authors.lastname: Liu
authors.forename: C A
authors.initials: CA
authors.lastname: Stewart
authors.forename: R L
authors.initials: RL
authors.lastname: King
authors.forename: D A
authors.initials: DA
authors.lastname: Danner
authors.forename: R T
authors.initials: RT
authors.lastname: Dell'Orco
authors.forename: J K
authors.initials: JK
authors.lastname: McClung
mesh_terms: D002453:Cell Cycle; D002478:Cells, Cultured; D016922:Cellular Senescence; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D000091402:Prohibitins; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins
chemical_list: D000091402:Prohibitins; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins