Cell-substrate adhesion and metastatic potential of cultured mesothelioma cells induced by asbestos.
Cell-substrate adhesion was quantified for two cultured mesothelioma cell lines (epitheliomatous and sarcomatous) on glass, fibronectin and laminin substrates. Interference reflection microscopy (IRM) was used to image the adhesion patterns of cells and a grey level analysis was employed to quantify adhesion. Sarcomatous cells demonstrated marked adhesion to glass and fibronectin-coated substrates but not to laminin-coated substrate, with the greatest adhesion occurring on the fibronectin-coated surface. This adhesion was accompanied by cytoplasmic spreading. By contrast, epitheliomatous cells showed little tendency to adhere to any of the substrates and only showed significant spreading when in contact with the laminin substrate (P < 0.01). A bioassay was used to determine the metastatic potential of each of the cell lines. Via the intravenous route, the sarcomatous cells killed the host rats in 24.7 +/- 1.5 (S.D.) days compared to 27.3 +/- 0.9 (S.D.) days for the epitheliomatous cells (P < 0.01). After subcutaneous inoculation of tumour cells, the sarcomatous cells killed the host rats in 54.7 +/- 0.7 (S.D.) days compared to 48.5 +/- 0.5 (S.D.) days for the epitheliomatous cells (P < 0.01). We conclude that the results of the metastasis bioassays were consistent with the predicted behavior of these cell lines based on their ability to adhere to substrates in the in vitro adhesion assays.
Lee
M M
MM
Department of Medical Physiology, University of Calgary, AB, Canada.
Green
F H
FH
Schoel
W M
WM
Schürch
S
S
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005353:Fibronectins; D005898:Glass; D007797:Laminin; D008297:Male; D008654:Mesothelioma / Q000473:pathology* / Q000648:ultrastructure; D009362:Neoplasm Metastasis; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D004273:DNA, Neoplasm; D005353:Fibronectins; D007797:Laminin; D001194:Asbestos