Expression of a yeast intron-containing tRNA in the archaeon Haloferax volcanii.
Expression of the yeast tRNAPro(UGG) gene in Haloferax volcanii resulted in the production of a single stable transcript that had not undergone intron processing or processing of 5' and 3' flanking sequences. Mutation of the exon-intron boundary region of this RNA to produce a precursor RNA with the preferred halobacterial consensus exon-intron boundary structure did not restore intron processing. Processing of 5' and 3' flanking sequences was restored when the acceptor stem U6-U67 pair was changed to A6-U67. The significance of these results in defining the recognition requirements of tRNA maturation enzymes in the halophilic domain Archaea is discussed.
Palmer
J R
JR
Department of Microbiology, Ohio State University, Columbus 43210.
Nieuwlandt
D T
DT
Daniels
C J
CJ
D001483:Base Sequence; D006216:Halobacteriaceae / Q000235:genetics / Q000378:metabolism*; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012323:RNA Processing, Post-Transcriptional; D012361:RNA, Transfer, Pro / Q000096:biosynthesis* / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012361:RNA, Transfer, Pro