Oligo[d(C).(G)] runs exhibit a helical repeat of 11.1 bp in solution and cause slight DNA curvature when properly phased.
We have inserted d(C)10 in a set of DNA fragments with bent segments on both ends, which are rotated with respect to each other by base pair wise increasing insertions. The electrophoretic mobilities on polyacrylamide gels of these DNA fragments were used to identify insertion sizes with cis conformations of the bent ends. These experiments revealed a helical repeat in solution of d(C).d(G) tracts of 11.1 +/- 0.08 bp. The electrophoretic mobilities of ligation ladders with properly phased d(C)5 and d(C)16 runs demonstrate a small but clearly detectable curvature of these fragments.
Biburger
M
M
Institut für Mikrobiologie und Biochemie, Friedrich-Alexander Universität Erlangen-Nürnberg, Germany.
Niederweis
M
M
Hillen
W
W
D001483:Base Sequence; D004247:DNA; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011066:Poly C / Q000235:genetics*; D011068:Poly G / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012996:Solutions
D009838:Oligodeoxyribonucleotides; D012996:Solutions; D011068:Poly G; C008961:poly G-poly C; D011066:Poly C; D004247:DNA