A bioassay for the detection of tumor necrosis factor from eight different species: evaluation of neutralization rates of a monoclonal antibody against human TNF-alpha.
We have evaluated a recently developed bioassay based on porcine kidney (PK(15)) cells for the detection of tumor necrosis factor-alpha (TNF-alpha) from eight different species. This test could also be used to measure human TNF-beta with similar sensitivity when compared to the widely used L929 bioassay.
Pauli
U
U
Institute of Veterinary Virology, University of Bern, Switzerland.
Bertoni
G
G
Duerr
M
M
Peterhans
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D004285:Dogs; D005069:Evaluation Studies as Topic; D006041:Goats; D006736:Horses; D006801:Humans; D007700:Kinetics; D051379:Mice; D009500:Neutralization Tests; D012680:Sensitivity and Specificity; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha