An extrapancreatic action of diazoxide to inhibit glucose transport activity on adipocytes.
The effect of diazoxide on 3-O-methylglucose (3-O-MG) transport was studied in isolated rat adipocytes to elucidate its extrapancreatic action. Diazoxide (0.3-3 mmol/L) significantly inhibited 3-O-MG uptake into adipocytes in a basal state or an insulin-stimulated state. The inhibitory effect was mainly due to the inhibition of insulin responsiveness for 3-O-MG uptake. The insulin responsiveness is determined by the capacity in the process of insulin action and in the final glucose transport activity, and diazoxide mainly inhibited the 3-O-MG transport activity itself. Based on these findings, this extrapancreatic action of diazoxide is considered to contribute partially to raising the blood glucose level in children receiving the drug. Diazoxide, as a glucose transport inhibitor, may be a useful tool for studying the issues related to glucose transport or insulin action.
Goto
Y
Y
Department of Pediatrics, Ehime University School of Medicine, Japan.
Kida
K
K
Kaino
Y
Y
Ito
T
T
Matsuda
H
H
D019325:3-O-Methylglucose; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003981:Diazoxide / Q000494:pharmacology*; D008757:Methylglucosides / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar
D008757:Methylglucosides; D019325:3-O-Methylglucose; D003981:Diazoxide