Establishment of cell lines from bovine brain.
As shown by immunocytochemistry, 16 cell lines of neuronal, oligodendroglial or neuronoglial origin have been established from bovine fetal brain by immortalization with SV40 virus and cloning in soft agar. The cell lines were characterized according to their cell surface markers using mono- and polyclonal antibodies.
Uysal
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Institute for Medical Microbiology, Veterinary Faculty, Ludwig-Maximilians-University Munich, Federal Republic of Germany.
Kaaden
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D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001921:Brain / Q000166:cytology* / Q000196:embryology; D002417:Cattle; D002460:Cell Line / Q000276:immunology; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D005333:Fetus / Q000166:cytology; D007150:Immunohistochemistry; D013539:Simian virus 40
D000954:Antigens, Surface