Cloning and expression of a cDNA encoding the homologue of Ran/TC4 GTP-binding protein from Plasmodium falciparum.
In our effort to identify and study proteins that are important for the progression of the malaria parasite, Plasmodium falciparum, through the cell cycle, we have cloned and sequenced the homologue of Ras-related nuclear protein Ran/TC4 (PfRan). The predicted peptide sequence of PfRan is 214 amino acids long and contains consensus motifs of the Ras superfamily. The expression of a 1.7 kb PfRan transcript changes during the cell cycle, reaching a peak at the trophozoite stage of growth of the parasite. The recombinant PfRan binds GTP similar to other Ras-like proteins.
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Division of Infectious Diseases, College of Medicine, University of Florida, Gainesville 32611.
Chakrabarti
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D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics*; D017125:Genes, Protozoan; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020931:ran GTP-Binding Protein
D018076:DNA, Complementary; D009687:Nuclear Proteins; D019204:GTP-Binding Proteins; D020931:ran GTP-Binding Protein