MyoD transactivates angiotensinogen promoter in fibroblast C3H10T1/2 cells.
MyoD is one of the regulatory genes that causes activation of the expression of muscle specific genes in fibroblasts through interaction with a cis-acting DNA element CANNTG (E-box). We show here that forced expression of MyoD in mouse fibroblast C3H10T1/2 cells induces the expression of the liver specific rat angiotensinogen gene promoter. In the absence of MyoD, the angiotensinogen promoter is not expressed in C3H10T1/2 fibroblasts. Cotransfection of MyoD has no significant effect on the angiotensinogen promoter activity in the liver-derived HepG2 cells, suggesting that the regulatory programs of HepG2 and C3H10T1/2 cells are different, at least in terms of the requirement for MyoD-mediated transactivation of the target angiotensinogen promoter.
Goswami
S K
SK
Department of Cell Biology and Anatomy, SUNY Health Science Center, Brooklyn 11203.
Zhao
Y Y
YY
Siddiqui
M A
MA
Kumar
A
A
D000808:Angiotensinogen / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D005347:Fibroblasts / Q000378:metabolism*; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000235:genetics; D017570:MyoD Protein / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D009124:Muscle Proteins; D017570:MyoD Protein; D011993:Recombinant Fusion Proteins; D000808:Angiotensinogen