title: Oocyte recovery and maturation in the American black bear (Ursus americanus): a model for endangered ursids.
abstract: A study was conducted to determine if meiotic maturation could be induced in ovarian oocytes of the American black bear (Ursus americanus), a model for gamete "rescue" techniques for endangered ursids. Ovaries obtained from 48 black bears yielded 2,403 oocytes (51.1 +/- 4.9/female), of which 777 (32.3%) were morphologically classified as excellent quality. More total oocytes were recovered from donors that were anestrous compared to luteal/pregnant (P < 0.05) at the time of ovarian excision. Delaying the recovery of oocytes from antral follicles within excised ovaries from 12-24 hr to 25-36 hr had no effect (P > 0.05) on the overall number of high quality oocytes recovered or subsequent maturational ability. The highest incidence of metaphase II was reached between 48 and 60 hr of in vitro incubation. Donor status (anestrous vs. luteal/pregnant) had no influence on the oocyte maturation rate by 24 or 48 hr, but by 60 hr, more (P < 0.05) oocytes recovered from anestrous females (43.9%) had achieved metaphase II compared to luteal/pregnant counterparts (23.1%). In preliminary trials involving endangered ursids, 54 ovarian oocytes were recovered from three aged sun bears (Helarctos malayanus), of which 72.2% were excellent quality and 15.4% matured in vitro to metaphase II. Similarly, 119 antral oocytes were recovered from two aged sloth bears (Melursus ursinus), of which 41.2% were excellent and 17.5% matured in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Omaha's Henry Doorly Zoo, Nebraska 68107.
authors.forename: L A
authors.initials: LA
authors.lastname: Johnston
authors.forename: A M
authors.initials: AM
authors.lastname: Donoghue
authors.forename: W
authors.initials: W
authors.lastname: Igo
authors.forename: L G
authors.initials: LG
authors.lastname: Simmons
authors.forename: D E
authors.initials: DE
authors.lastname: Wildt
authors.forename: J
authors.initials: J
authors.lastname: Rieffenberger
mesh_terms: D000375:Aging; D000818:Animals; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D066298:In Vitro Techniques; D009865:Oocytes / Q000254:growth & development*; D009866:Oogenesis; D001503:Ursidae