Interplay between differentiation and cell cycle arrest at G1 in phorbol diester-treated human myelocytic leukemia cells.
The cell cycle arrest at G1 of myelocytic leukemia cells by incubation with phorbol diesters was examined in HL-60 cells and more mature THP-1 cells. We found: (1) induction of phenotypic parameters of differentiation prior to G1 arrest; (2) a long induction period in HL-60 cells compared to zero in THP-1 cells; and (3) strong inhibition of G1 arrest with a protein kinase C inhibitor in THP-1 cells. We conclude, from these lines of evidence and some additional information, that differentiation induces G1 arrest. At present, the most plausible causative molecule is Mac-1.
Oda
T
T
Life Science Group, Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
Kitajima
K
K
Hirano
T
T
Shimizu
N
N
Utiyama
H
H
D002454:Cell Differentiation / Q000187:drug effects; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D010703:Phorbol Esters; D015240:Phorbol 12,13-Dibutyrate; D013755:Tetradecanoylphorbol Acetate