Oxidation of low molecular weight chloroalkanes by cytochrome P450CAM.
Cytochrome P450CAM from Pseudomonas putida G786 oxidized chlorinated ethanes and 1,2-dichloropropane. The rate of NADH oxidation decreased with decreasing chlorine substitution. The single detectable oxidation products of 1,1,1-trichloroethane and 1,2-dichloropropane were 1,1,1-trichloroethanol and chloroacetone, respectively. Organic product formation was largely uncoupled from NADH oxidation.
Lefever
M R
MR
Department of Biochemistry, Gortner Laboratory, University of Minnesota, St. Paul 55108.
Wackett
L P
LP
D019475:Camphor 5-Monooxygenase; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D016958:Pseudomonas putida / Q000201:enzymology*; D013379:Substrate Specificity
D006843:Hydrocarbons, Chlorinated; D009243:NAD; D003577:Cytochrome P-450 Enzyme System; D006861:Hydrogen Peroxide; D006899:Mixed Function Oxygenases; D019475:Camphor 5-Monooxygenase