title: Partial purification of two proteins which sensitize ribosomes to gelonin: sensitization is not linked to phosphorylation of ribosomal proteins.
abstract: Inactivation of ribosomes by gelonin, from Gelonium multiflorum, requires ATP and extraribosomal protein(s) present in the rabbit reticulocyte lysate [SPERTI, S. et al. (1991) Biochem. J. 277, 281-284]. On the anion exchanger Mono Q the activity responsible for the sensitization of ribosomes to gelonin resolves in two peaks which both display a kinase activity on ribosomal proteins. However, staurosporin, an inhibitor of several protein kinases, strongly inhibits phosphorylation of ribosomal proteins without affecting the gelonin-promoting activity of Mono Q peaks. All the evidence collected contradicts a direct link between sensitization to gelonin and phosphorylation of ribosomes.
authors.affiliation: Dipartimento di Patologia sperimentale, Università di Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Brigotti
authors.forename: S
authors.initials: S
authors.lastname: Sperti
authors.forename: D
authors.initials: D
authors.lastname: Carnicelli
authors.forename: L
authors.initials: L
authors.lastname: Montanaro
mesh_terms: D000818:Animals; D047388:Casein Kinases; D033364:Decapoda; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012269:Ribosomal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000201:enzymology / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D012269:Ribosomal Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum; D011494:Protein Kinases; D047388:Casein Kinases