Dexamethasone down-regulates endothelin receptors in human cerebromicrovascular endothelial cells.
Human cerebromicrovascular endothelial cells (HBEC) in culture express high affinity ETA receptors coupled to phospholipase C activation. Pretreatment of HBEC with 1 microM dexamethasone for 24 h decreased the number of the ET-1 binding sites (Bmax) on HBEC (96 fmol/mg protein vs 57 fmol/mg protein) without changing the binding affinity (KD) (101 pM vs 92 pM) or displacing profile (ET-1 = ET-2 > ET-3 > S6c). Dexamethasone-pretreated HBEC also exhibited a 40% reduction in the maximal ET-1-stimulated inositol triphosphate (IP3) production, whereas half-maximal stimulatory concentration (EC50) was not affected. This effect of dexamethasone was concentration-dependent, and most pronounced after 24 h of pretreatment. The inhibitory effect of dexamethasone on the ET-1-induced IP3 production was abolished by glucocorticoid-receptor antagonist cortexolone. In contrast, vasopressin-mediated IP3 response in HBEC was not changed by dexamethasone. Cyclo-oxygenase inhibitors indomethacin and acetylsalicylic acid did not influence the ET-1-induced IP3 production by HBEC. The down-regulation of ETA receptors in HBEC by dexamethasone, may represent one of the mechanisms involving the described effects of glucocorticoids on cerebromicrovascular function (i.e. changes in blood brain barrier properties, secretion of vasoactive factors, vascular morphogenesis).
Stanimirovic
D B
DB
Stroke Branch, National Institute for Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
McCarron
R M
RM
Spatz
M
M
D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000502:physiology; D003348:Cortisone / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects*; D016232:Endothelins / Q000378:metabolism / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007293:Inosine Triphosphate / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007457:Iodine Radioisotopes; D017466:Receptors, Endothelin / Q000187:drug effects*; D011965:Receptors, Glucocorticoid / Q000187:drug effects
D016861:Cyclooxygenase Inhibitors; D016232:Endothelins; D007457:Iodine Radioisotopes; D017466:Receptors, Endothelin; D011965:Receptors, Glucocorticoid; D007293:Inosine Triphosphate; D007294:Inositol; D003907:Dexamethasone; D003348:Cortisone