Human immunodeficiency virus-1 recombinant gp120 induces changes in protein kinase C isozymes--a preliminary report.
Human immunodeficiency virus 1 (HIV-1) and its purified proteins activate target cell functions. Because protein kinase C (PKC) plays a crucial role in signal transduction and there is a molecular heterogeneity of PKC, we compared the effect of recombinant HIV-1 gp120 and phorbol ester (PMA) on PKC isozymes in monocytic U937 cells, with isozyme-specific antibodies using flow cytometry. All PKC isozymes except PKC-gamma were present in U937 cells. Both PMA and HIV-1 gp120 increased levels of calcium-dependent and -independent PKC isozymes. The most striking change was observed in PKC-zeta isozymes levels. This study for the first time demonstrates that HIV-1 gp120 affects calcium-independent PKC isozymes in U937 cells.
Gupta
S
S
Division of Basic and Clinical Immunology, University of California, Irvine 92717.
Aggarwal
S
S
Kim
C
C
Gollapudi
S
S
D004789:Enzyme Activation; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology*; D015497:HIV-1; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000187:drug effects*; D010703:Phorbol Esters / Q000494:pharmacology; D011493:Protein Kinase C / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D015699:HIV Envelope Protein gp120; D007527:Isoenzymes; D010703:Phorbol Esters; D011994:Recombinant Proteins; D011493:Protein Kinase C