Analysis and sorting of chromosomes by flow cytometry: new trends.
Flow cytogenetic is widely used since 1975, and essentially contributes to karyotype analysis and chromosome sorting. The principles of experimentation and its possibilities and limitations are now well known. Recently several new technologies have appeared. What attitude should the cytometrist adopt regarding PCR, microdissection of chromosomes, in situ hybridization, slit-scan flow cytometry or image analysis?
Métézeau
P
P
Unité de Biochimie Cellulaire, INSERM-Pasteur et CNRS URA 1129, Institut Pasteur, Paris.
Schmitz
A
A
Frelat
G
G
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002875:Chromosomes; D002877:Chromosomes, Human; D003582:Cytogenetics / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D004247:DNA / Q000032:analysis; D005057:Eukaryotic Cells / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D006801:Humans; D007621:Karyotyping / Q000379:methods; D008297:Male; D008846:Micromanipulation; D013048:Specimen Handling
D004247:DNA