title: The involvement of TGF beta 1 in early avian development: gastrulation and chondrogenesis.
abstract: We examined the effects of transforming growth factor-beta 1 (TGF beta 1) and a neutralizing monoclonal antibody on two phases of early chick embryo development: gastrulation and chondrogenesis. We carried out experiments in vivo and in vitro on mesoderm cells from the gastrulating embryo at day 1, and on sclerotome cells from day 3 embryos, having previously shown that this factor is present among these cells at these stages of development. Addition of the antibody to cultures of these cells produced a dose-dependent decrease in cell out-growth and spreading and concomitantly reduced fibronectin deposition. In vivo studies of the effects of TGF beta 1 on mesoderm during gastrulation were carried out by grafting beads carrying this agent into gastrulating embryos. We used beads of ion-exchange resin as well as hydrolysed polyacrylamide, and found that the grafts produced an accumulation of mesoderm cells around the implant and, at later stages, the formation of enlarged somites. There was no effect on embryonic axis formation. Studies of bromodeoxyuridine (BrdU) incorporation indicated that the mesoderm accumulation was due, at least in part, to an increase in cell proliferation. However, examination of the effect of TGF beta 1 on BrdU incorporation by mesoderm during gastrulation and sclerotome cells in vitro indicated in inhibition of cell proliferation, an inconsistency explained in terms of the variation between the in vivo and in vitro conditions. We conclude that TGF beta 1 is both appropriately located, and is able, to influence cell proliferation among the mesodermal cell populations during early development, and that this effect contributes to the overall control of mesodermal morphogenesis.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, University of Alberta, Edmonton, Canada.
authors.forename: E J
authors.initials: EJ
authors.lastname: Sanders
authors.forename: S
authors.initials: S
authors.lastname: Prasad
authors.forename: N
authors.initials: N
authors.lastname: Hu
mesh_terms: D000818:Animals; D002356:Cartilage / Q000196:embryology*; D002455:Cell Division; D002642:Chick Embryo / Q000166:cytology / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology; D005775:Gastrula / Q000502:physiology*; D008648:Mesoderm / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000502:physiology*
chemical_list: D016212:Transforming Growth Factor beta