title: Dynamin binds to SH3 domains of phospholipase C gamma and GRB-2.
abstract: Src homology 3 (SH3) domains are found in a variety of proteins that are involved in signal transduction or represent components of the cytoskeleton. These domains are thought to serve as modules that mediate specific protein-protein interactions that include proline-rich sequences on the target protein. We have identified proteins of 110, 80, 65, and 43 kDa in human embryonic fibroblasts that bind specifically to the SH3 domain of phospholipase C gamma, a primary substrate of receptor tyrosine kinases, and characterized the 110-kDa band as the microtubule-activated GTPase dynamin. In addition, dynamin binds the son of sevenless adaptor protein GRB-2 with even higher affinity. This interaction does not require the dynamin GTPase function and involves a proline-rich target sequence between residues 812 and 820 of dynamin.
authors.affiliation: Department of Molecular Biology, Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Seedorf
authors.forename: G
authors.initials: G
authors.lastname: Kostka
authors.forename: R
authors.initials: R
authors.lastname: Lammers
authors.forename: P
authors.initials: P
authors.lastname: Bashkin
authors.forename: R
authors.initials: R
authors.lastname: Daly
authors.forename: W H
authors.initials: WH
authors.lastname: Burgess
authors.forename: A M
authors.initials: AM
authors.lastname: van der Bliek
authors.forename: J
authors.initials: J
authors.lastname: Schlessinger
authors.forename: A
authors.initials: A
authors.lastname: Ullrich
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D034281:Dynamins; D051380:GRB2 Adaptor Protein; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D016391:Genes, src; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008870:Microtubules / Q000201:enzymology*; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D011506:Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D010738:Type C Phospholipases; D020558:GTP Phosphohydrolases; D034281:Dynamins