title: Analysis of a desiccation and ABA-responsive promoter isolated from the resurrection plant Craterostigma plantagineum.
abstract: The resurrection plant Craterostigma plantagineum can recover from severe desiccation within 24 h of contact with water, and it is used as a model system to analyse desiccation tolerance in higher plants. During drying or ABA treatment a specific set of transcripts accumulates rapidly in leaves and other tissues. In order to study transcriptional mechanisms of stress-induced gene expression one gene (CDeT27-45) was selected for promoter analysis. Chimeric gene fusions were constructed of the CDeT27-45 promoter and beta-glucuronidase or luciferase. These constructs were tested in a homologous transient expression system which allowed the identification of promoter elements conferring ABA inducibility. By introducing the chimeric gene fusions into tobacco via Agrobacterium-mediated transformation we found that the promoter activity is under strict tissue-specific and developmental control. In tobacco the promoter was only active in developing embryos and in mature pollen grains-two tissues which are naturally desiccation tolerant in tobacco. The specific temporal expression pattern was attributed to particular 5' upstream sequences. The promoter analysis presented here should allow the separation of important regulatory components as a first step in dissecting events in the signal transduction chain.
authors.affiliation: Max Planck Institut für Züchtungsforschung, Köln, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Michel
authors.forename: F
authors.initials: F
authors.lastname: Salamini
authors.forename: D
authors.initials: D
authors.lastname: Bartels
authors.forename: P
authors.initials: P
authors.lastname: Dale
authors.forename: M
authors.initials: M
authors.lastname: Baga
authors.forename: A
authors.initials: A
authors.lastname: Szalay
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D003890:Desiccation; D005786:Gene Expression Regulation / Q000187:drug effects; D017343:Genes, Plant; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D010944:Plants / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000187:drug effects; D015183:Restriction Mapping; D014867:Water / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D014867:Water; D000040:Abscisic Acid