[Muscle tissue for diagnosis of neuromuscular diseases. Procedures of specimen taking, transport and preparation].
In this review we describe surgical methods for open muscle biopsy. In order to obtain optimal tissue quality, correct handling of the muscle specimens at all stages of the procedure is essential. A technologist who is familiar with the procedure should be present in the operating room. This is important to ensure that specimens for histochemical or biochemical studies are frozen immediately and specimens for electron microscopy are fixed without delay. We describe procedures for processing the specimens for cryostat sections, paraffin sections and electron microscopy, and a simple method for handling muscle specimens in local hospitals before transfer to a hospital with a neuropathological service.
Lindal
S
S
Patologisk anatomisk avdeling, Regionsykehuset i Tromsø.
Lund
I
I
Lorentzen
M A
MA
Tennøe
T
T
Skullerud
K
K
Lindboe
C F
CF
D001706:Biopsy / Q000379:methods*; D001707:Biopsy, Needle / Q000379:methods; D006801:Humans; D009132:Muscles / Q000378:metabolism / Q000473:pathology*; D009468:Neuromuscular Diseases / Q000473:pathology*; D013048:Specimen Handling / Q000379:methods*; D014021:Tissue Preservation / Q000379:methods
Muskelvev for diagnostikk av neuromuskulaere sykdommer. Prosedyre for prøvetaking, transport og preparering.