title: Pulmonary intravascular macrophages in horses and ponies.
abstract: Seven horses (4 anesthetized and 3 awake) and 2 ponies (anesthetized) were studied to evaluate the high sensitivity of the pulmonary circulation of the horse to various blood-borne particles, and to establish the presence of intravascular macrophages in the lung. Pulmonary and systemic pressures and cardiac output before and during particle injection were measured in some animals. An anesthetized foal had a large increase in pulmonary arterial pressure (32 and 34 mm of Hg) within 1 minute of IV administration of small test doses of radioactively labeled liposomes (2.5 mumol/kg of body weight) or a 1% suspension of blue pigment (0.3 ml/kg), respectively. Quantitative real-time gamma camera imaging of the foal revealed high retention of the labeled liposomes during the first pass through the lungs; retention persisted throughout the experiment. Postmortem analysis revealed 55 and 47% lung retention of liposomes and blue pigment, respectively. The 2 anesthetized ponies had increased pulmonary artery pressure of 34 +/- 7 mm of Hg, decreased cardiac output, and 42% lung retention after administration of 1% blue pigment (0.2 ml/kg), whereas 3 awake horses had increased pressure of 28 +/- 9 mm of Hg after 1.8 x 10(8) (1.8-microns-diameter) latex microspheres/kg. None of the injected particles caused vascular obstruction, and they do not cause pulmonary vascular reactivity in species that lack pulmonary intravascular macrophages. Finally, 3 horses (1 anesthetized and 2 awake) were infused IV with small doses of the blue pigment, and their lungs were perfusion-fixed to identify specific labeling of the pulmonary intravascular macrophages.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: K E
authors.initials: KE
authors.lastname: Longworth
authors.forename: K A
authors.initials: KA
authors.lastname: Jarvis
authors.forename: W S
authors.initials: WS
authors.lastname: Tyler
authors.forename: E P
authors.initials: EP
authors.lastname: Steffey
authors.forename: N C
authors.initials: NC
authors.lastname: Staub
mesh_terms: D000375:Aging / Q000502:physiology*; D000768:Anesthesia, General; D000818:Animals; D001794:Blood Pressure; D002302:Cardiac Output; D004337:Drug Carriers; D004562:Electrocardiography; D006736:Horses / Q000502:physiology*; D007205:Indium Radioisotopes; D008051:Lipid Bilayers; D008168:Lung / Q000166:cytology / Q000648:ultrastructure; D008264:Macrophages / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular; D011651:Pulmonary Artery / Q000502:physiology*; D011652:Pulmonary Circulation; D014851:Wakefulness
chemical_list: D004337:Drug Carriers; D007205:Indium Radioisotopes; D008051:Lipid Bilayers