Assessment of an anti-T-cell receptor variable region antibody panel in cutaneous T-cell lymphoma.
Using a panel of seven commercially available monoclonal antibodies directed against specific sites of the T-cell receptor variable region, we have studied the staining patterns in 15 biopsies from 11 patients with cutaneous T-cell lymphoma (CTCL), and in five patients with benign lymphocytic infiltrates. Eight of the 11 patients with CTCL showed staining of the majority of lymphocytes with either V beta 8 alone (n = 3), V beta 12 alone (n = 2) or with both V beta 8 and V beta 12 (n = 3). None of the biopsies from patients with benign dermatoses showed staining of the majority of lymphocytes with any of the antibodies.
McHenry
P M
PM
Department of Dermatology, University of Glasgow, Robertson Institute, U.K.
Campbell
I
I
Mackie
R M
RM
D000911:Antibodies, Monoclonal; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D007150:Immunohistochemistry; D016410:Lymphoma, T-Cell, Cutaneous / Q000175:diagnosis / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D012871:Skin Diseases / Q000276:immunology; D012878:Skin Neoplasms / Q000175:diagnosis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D011948:Receptors, Antigen, T-Cell