Effect of superoxide dismutase and catalase on the nephrotoxicity induced by subchronical administration of ochratoxin A in rats.
Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus ochraceus as well as other molds. It is a natural contaminant of mouldy food and feed. OTA has a number of toxic effects, the most prominent being nephrotoxicity. Furthermore, OTA is immunosuppressive, genotoxic, teratogenic and carcinogenic. OTA inhibits protein synthesis by competition with phenylalanine in the phenylalanine-tRNA aminoacylation reaction. Recently, lipid peroxidation induced by OTA has been reported, indicating that the lesions induced by this mycotoxin could be also related to oxidative pathways. It was then interesting to study effects of the superoxide dismutase (SOD) and catalase on the nephrotoxicity induced by OTA in rats. The two enzymes (20 mg/kg body weight each) were given to rats by subcutaneous injection, every 48 h, 1 h before gavage by OTA (289 micrograms/kg b.w. every 48 h), for 3 weeks. SOD and catalase prevented most of the nephrotoxic effects induced by ochratoxin A, observed as enzymuria, proteinuria, creatinemia and increased urinary excretion of OTA. Altogether these results indicate (i) that superoxide radicals and hydrogen peroxide are likely to be involved in the damaging processes of OTA in vivo, (ii) that SOD and catalase might be used for prevention of renal lesions in cases of ochratoxicosis.
Baudrimont
I
I
Laboratoire de Toxicologie et d'Hygiène Appliquée, Université de Bordeaux II, France.
Betbeder
A M
AM
Gharbi
A
A
Pfohl-Leszkowicz
A
A
Dirheimer
G
G
Creppy
E E
EE
D000284:Administration, Oral; D000818:Animals; D002374:Catalase / Q000494:pharmacology / Q000627:therapeutic use*; D003404:Creatinine / Q000097:blood / Q000652:urine; D004798:Enzymes / Q000652:urine; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control; D007677:Kidney Function Tests; D015227:Lipid Peroxidation; D008297:Male; D009793:Ochratoxins / Q000037:antagonists & inhibitors* / Q000493:pharmacokinetics / Q000633:toxicity*; D011507:Proteinuria / Q000139:chemically induced / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000494:pharmacology / Q000627:therapeutic use*
D004798:Enzymes; D009793:Ochratoxins; C025589:ochratoxin A; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase