title: Synergism of tocopherol and ascorbate on the survival of cultured brain neurones.
abstract: The effects of antioxidants tocopherols and ascorbic acid were tested on the survival of hippocampal and striatal neurones in dissociated culture. Alpha- and gamma-tocopherol increased the number of surviving neurones in a concentration-dependent manner. Significant effect was observed at concentrations of 10(-8)-10(-6) M. Furthermore, the promoting effect of alpha-tocopherol on neuronal survival was markedly enhanced in the presence of ascorbic acid (2 x 10(-6) M). These results indicate that tocopherols and ascorbic acid support the survival of cultured neurones by protecting them from oxidative attack.
authors.affiliation: Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Katsuki
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology; D003470:Culture Media; D004357:Drug Synergism; D006624:Hippocampus / Q000166:cytology; D007536:Isomerism; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D014810:Vitamin E; D001205:Ascorbic Acid