Structure elucidation of glykenin glycosidic antibiotics from Basidiomycetes sp. V. High-performance liquid chromatographic separation of components of glykenin.
The glycosidic antibiotics of the glykenin (GK) family produced by Basidiomycetes sp. were separated into nine components (GK-I-VII and DG) by normal-phase chromatography. It was found that these components differ in the number and location of the acetyl groups in the sugar moiety. Each component (GK-I-VII and DG) was further separated into three isomers (A, B and C), which possess different aglycones, by reversed-phase chromatography on an ODS column with methanol-acetonitrile as eluent. The best composition of the eluent was found to be methanol-acetonitrile-1% trifluoroacetic acid (4:3.5:2.5). The profile analysis of GK-III-VII and DG was also carried out using a modified mobile phase. The combination of normal- and reversed-phase chromatography separated all components of the GK mixture except GK-I and II. The relationship between structure and separation behaviour of GK is discussed.
Nishida
F
F
Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Nishimura
M
M
Harada
K
K
Suzuki
M
M
Meevootisom
V
V
Flegel
T W
TW
Thebtaranonth
Y
Y
Intararuangsorn
S
S
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D001487:Basidiomycota / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013237:Stereoisomerism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C069643:glykenin IV