title: Cyclosporine A monitoring in patients with renal, cardiac, and liver transplants: a comparison between fluorescence polarization immunoassay and two different RIA methods.
abstract: In the present study a new method for selectively determining parent cyclosporine (CsA) in whole blood, a fluorescence polarization immunoassay (FPIA; TDx Abbott), was compared with a RIA method (Sandimmun, Sandoz Ltd, Basle, Switzerland). A total of 974 samples were collected during the first 3 post-operative months from 63 renal, cardiac, and liver transplant recipients. The CsA concentrations measured with FPIA ranged from 14% to 19% above RIA (specific) in the middle ranges. Regression equations in renal transplants: FPIA = 1.001 x RIA + 28; in heart transplants: FPIA = 1.08 x RIA + 27 and in liver transplants: FPIA = 1.13 x RIA + 13. Considering the improved precision of the new method (inter-assay CV with FPIA: 3.8-9.5%; with RIA: 18.6%), the slightly lower specificity will usually be of minor importance in the therapeutic range for whole blood CsA concentrations following organ transplantations. The FPIA measurements which deviated most from the regression line compared with RIA-specific CsA values, tended to coincide with high CsA concentrations or rather extreme RIA specific to RIA non-specific ratios. In addition to analytical imprecision with the RIA-specific method, lower specificity of the FPIA vs. some of the metabolites may explain these deviations. The majority of these observations occurred as isolated episodes with normal relationship between RIA specific and FPIA on preceding and following days. Accordingly large dosage adjustments should await verification in repeated samples. Following these precautions the FPIA method may prove useful and safe in the monitoring of cyclosporine treatment.
authors.affiliation: Institute of Clinical Biochemistry, National Hospital, Rikshospitalet, University of Oslo, Norway.
authors.forename: S
authors.initials: S
authors.lastname: Bergan
authors.forename: H E
authors.initials: HE
authors.lastname: Rugstad
authors.forename: O
authors.initials: O
authors.lastname: Stokke
authors.forename: O
authors.initials: O
authors.lastname: Bentdal
authors.forename: T
authors.initials: T
authors.lastname: Frøysaker
authors.forename: A
authors.initials: A
authors.lastname: Bergan
mesh_terms: D016572:Cyclosporine / Q000097:blood*; D016903:Drug Monitoring; D016231:Fluorescence Polarization Immunoassay; D016027:Heart Transplantation; D006801:Humans; D016030:Kidney Transplantation; D016031:Liver Transplantation; D016377:Organ Transplantation; D011863:Radioimmunoassay
chemical_list: D016572:Cyclosporine