High performance liquid chromatographic determination of levomepromazine in human breast milk and serum using solid phase extraction.
A high performance liquid chromatographic (HPLC) method has been developed for the determination of levomepromazine in human breast milk and serum. The levomepromazine was extracted by a rapid and simple extraction method using a Sep-Pak C18 cartridge. The extracts were separated by HPLC on a C8 bonded reversed phase column and detected by UV absorbance at 254 nm. There was no interference with endogenous substance in human breast milk and serum. A linear relationship was obtained for the levomepromazine over the concentration range of 10-300 ng/mL. The recoveries of levomepromazine added to human breast milk and serum were 92.5-99.1% and 86.9-103.9%, respectively.
Ohkubo
T
T
Department of Pharmacy, Hirosaki University Hospital, Japan.
Shimoyama
R
R
Sugawara
K
K
D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008728:Methotrimeprazine / Q000032:analysis* / Q000097:blood; D008895:Milk, Human / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D008728:Methotrimeprazine