Methyl xanthine and altered biomembrane dynamics: demonstration of protein mobility and enzyme inhibition by caffeine in sperm model system.
Treatment of spermatozoa with 10 mM caffeine increases the rotational mobility of thiol-containing proteins in a defined micellar micro-environment. This is associated with inhibition of superoxide dismutase activity and augmented superoxide anion radical generation. Increased sperm competence in presence of caffeine in human oligospermia is explained by these observations. Inhibition of cyclic phosphodiesterase by caffeine is suggested to be part of the observed lattice-rearrangement.
Sinha
S
S
School of Life Sciences, Indore, India.
Kumar
P
P
Laloraya
M
M
D002110:Caffeine / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D006801:Humans; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008565:Membrane Proteins / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors; D013481:Superoxides / Q000378:metabolism
D008565:Membrane Proteins; D013438:Sulfhydryl Compounds; D013481:Superoxides; D002110:Caffeine; D013482:Superoxide Dismutase