title: A point mutation in the chloroplast rps12 gene from Nicotiana plumbaginifolia confers streptomycin resistance.
abstract: In an effort to understand the mechanism of streptomycin resistance in Nicotiana plumbaginifolia, we have sequenced the chloroplast rps12 gene, a potential molecular target. We report that a streptomycin-resistant mutant isolated from protoplast cultures of N. plumbaginifolia contains an A-to-G transition at nucleotide position 149 in exon 2 of the chloroplast rps12 gene. The detected point mutation predicts a substitution of arginine for lysine in a phylogenetically conserved region.
authors.affiliation: Institute of Life Science, National Tsing Hua University, Hsinchu, Taiwan, ROC.
authors.forename: C M
authors.initials: CM
authors.lastname: Hsu
authors.forename: W P
authors.initials: WP
authors.lastname: Yang
authors.forename: C C
authors.initials: CC
authors.lastname: Chen
authors.forename: Y K
authors.initials: YK
authors.lastname: Lai
authors.forename: T Y
authors.initials: TY
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism*; D004247:DNA; D004351:Drug Resistance / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010947:Plants, Toxic; D017354:Point Mutation; D012269:Ribosomal Proteins / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*
chemical_list: D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D004247:DNA; D013307:Streptomycin