Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin.
Jasplakinolide, a naturally occurring cyclic peptide from the marine sponge, Jaspis johnstoni, has both fungicidal and antiproliferative activity. We now report that this peptide is a potent inducer of actin polymerization in vitro. The peptide has a much greater effect on Mg(2+)-actin than on Ca(2+)-actin. Competitive binding studies using rhodamine-phalloidin suggest that jasplakinolide binds to F-actin competitively with phalloidin with a dissociation constant of approximately 15 nM. This compares favorably to the previously reported IC50 of 35 nM for the antiproliferative effect of jasplakinolide on PC3 prostate carcinoma cells. The binding curve suggests that nearest neighbor positive cooperativity influences the binding of jasplakinolide (and perhaps also phalloidin) to F-actin. These results imply that jasplakinolide may exert its cytotoxic effect in vivo by inducing actin polymerization and/or stabilizing pre-existing actin filaments.
Bubb
M R
MR
Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892.
Senderowicz
A M
AM
Sausville
E A
EA
Duncan
K L
KL
Korn
E D
ED
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D001704:Biopolymers; D003603:Cytotoxins / Q000494:pharmacology*; D047630:Depsipeptides; D007700:Kinetics; D010456:Peptides, Cyclic / Q000494:pharmacology; D010590:Phalloidine / Q000378:metabolism*; D011161:Porifera; D011817:Rabbits; D013050:Spectrometry, Fluorescence
D000199:Actins; D001704:Biopolymers; D003603:Cytotoxins; D047630:Depsipeptides; D010456:Peptides, Cyclic; C057531:jasplakinolide; D010590:Phalloidine