v-Src-induced transformation is inhibited by okadaic acid.
The tumor promoter okadaic acid is a potent inhibitor of the serine/threonine protein phosphatases 1 and 2A. Addition of okadaic acid to v-Src-transformed BALB/c 3T3 cells reverted them to a flat morphology, increased fibronectin levels in the extracellular matrix, reduced saturation density, and inhibited the formation of colonies in soft agar. The ability of v-Src-transformed cells to proliferate in low serum was also inhibited by okadaic acid. These data implicate serine/threonine phosphatases in v-Src-induced transformation.
Gupta
R W
RW
Institute for Biomolecular Structure and Function, Hunter College, City University of New York, New York 10021.
Joseph
C K
CK
Foster
D A
DA
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D002472:Cell Transformation, Viral / Q000187:drug effects* / Q000235:genetics; D004988:Ethers, Cyclic / Q000494:pharmacology*; D016391:Genes, src; D051379:Mice; D008807:Mice, Inbred BALB C; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*
D004988:Ethers, Cyclic; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases