Effect of a peptide from Panax ginseng on the proliferation of baby hamster kidney-21 cells.
An alkaline fraction separated by ion exchange chromatographies from the water extract of Panax ginseng root stimulated the proliferation of baby hamster kidney-21 cells. Separation of the alkaline fraction by MCI-gel CHP 20P column chromatography followed by dialysis provided an active material. By a reversed-phase HPLC the active material was separated into six fractions, and an active colorless compound 1 was obtained from fraction 2 in a pure state. Compound 1 was composed of the following amino acids; Gly, Arg, Glu, Val in a ratio of 1:1:1:1, and caused 20% enhancement of proliferation of BHK-21 cells at a concentration of 3.40 microM. On the basis of physical and spectral data the structure of compound 1 was established as a tetrapeptide, Gly-Arg-gamma-Glu-Val-NH2.
Yagi
A
A
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
Akita
K
K
Ueda
T
T
Okamura
N
N
Itoh
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D007668:Kidney; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D005894:Panax / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal
D009842:Oligopeptides; D010940:Plant Proteins