Halothane-induced cytotoxicity in isolated rat hepatocytes: an electron microscopic study.
To determine the direct cytotoxicity of halothane for liver cells, the effects of this agent on isolated rat hepatocytes were investigated by electron microscopy and by examining cell viability. Soon after treatment with lower concentrations of halothane (0.5% and 0.75%), there were marked changes in the plasma membrane. Cell viability was reduced to 70% of the control. At higher concentrations of halothane (1% and 3%), degeneration of mitochondria was noted. Cell viability was reduced to 40% of the control. Halothane, at lower concentrations, appeared to induce early morphological alterations of the plasma membrane, while higher concentrations damaged the organella.
Kinugawa
H
H
Department of Dental Anesthesiology, Hiroshima University Dental Hospital, Japan.
Kawahara
M
M
Amakata
Y
Y
Maeda
T
T
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006221:Halothane / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D051381:Rats; D017208:Rats, Wistar
D006221:Halothane