Immunohistochemical localization of glutathione peroxidase in infarcted human brain.
This is the first report which demonstrates the presence of glutathione peroxidase in the autopsied brain of 5 patients without cerebral infarction and 21 patients with cerebral infarction by the indirect enzyme-labeled antibody technique with monoclonal antibody to human glutathione peroxidase. In 2 out of 5 patients without cerebral infarction, a weak reaction for glutathione peroxidase was demonstrated both in neurons and glia. In 6 patients who had died within 5 days after stroke, no staining was observed in infarcted brain tissue except in macrophages. In all 15 patients who had died more than 6 days after stroke, however, a reaction for glutathione peroxidase was demonstrated in the cytoplasm of glial cells in the marginal area around the infarction, and there was a patchy reaction in the cytoplasm of macrophages in the core lesion. These results suggest that glutathione peroxidase in glial cells of the marginal area around the infarction may play a protective role against lipid peroxidation after cerebral infarction, or alternatively, may be involved in the healing process after ischemia.
Takizawa
S
S
Department of Neurology, Tokai University School of Medicine, Kanagawa, Japan.
Matsushima
K
K
Shinohara
Y
Y
Ogawa
S
S
Komatsu
N
N
Utsunomiya
H
H
Watanabe
K
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D002544:Cerebral Infarction / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005979:Glutathione Peroxidase / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase