Prostaglandin E2 production in submandibular salivary glands of rats in essential fatty acid deficiency.
Three groups of rats were fed an essential fatty acid (EFA)-deficient diet (EFAD), marginally EFA-deficient diet (MEFAD) or a control diet. Arachidonic acid levels in total phospholipids and the ex-vivo production of prostaglandin E2 (PGE2) in the presence of calcium ionophore were measured at 5 and 9 weeks in the submandibular salivary glands (SMSG). The arachidonic acid levels were significantly different among the 3 groups of rats fed the respective diets for 9 weeks. Ex-vivo PGE2 production was significantly decreased in the EFAD group but not in the MEFAD group as compared to the control group. The changes in fatty acid composition, arachidonic acid levels and ex-vivo production of PGE2 were reversed after 5 weeks of feeding the control diet to the EFAD or the MEFAD rats. Since arachidonic acid and PGE2 are involved in signal transduction pathways in the SMSG, an EFA deficiency is likely to modify these pathways.
Abdel-Hakim
S M
SM
Department of Biochemistry and Molecular Biology, LSU Medical Center, New Orleans 70119.
Alam
B S
BS
Alam
S Q
SQ
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage; D015232:Dinoprostone / Q000096:biosynthesis*; D005227:Fatty Acids / Q000378:metabolism; D005228:Fatty Acids, Essential / Q000172:deficiency*; D066298:In Vitro Techniques; D008297:Male; D009077:Mucins / Q000096:biosynthesis; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013363:Submandibular Gland / Q000378:metabolism*
D001095:Arachidonic Acids; D004041:Dietary Fats; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D009077:Mucins; D010743:Phospholipids; D015232:Dinoprostone