Protein tyrosine kinases transcribed in a murine thymic medullary epithelial cell line.
Medullary epithelial cells of the thymus can be activated by contact with thymocytes. We identified a number of protein tyrosine kinases (PTKs) that could be responsible for the tyrosine phosphorylation observed in a murine thymic medullary epithelial cell line (E-5) following complex formation with thymocytes. Degenerate oligodeoxyribonucleotides (oligos) derived from the amino acid (aa) sequence motifs of PTK catalytic domains were used as oligo primers for PCR amplification to determine the PTK genes which are normally transcribed in the E-5 cell line. Amplicons were cloned, sequenced and the deduced aa sequences were compared to known PTK sequences. Among the 13 distinct PTK catalytic domains identified in E-5 cells, two were novel: they were encoded by eteck, a member of the eph sub-family of PTKs, and thy, a member of the src sub-family of PTKs.
Hébert
B
B
Centre de recherche en immunologie, Institut Armand-Frappier, Laval, Québec, Canada.
Bergeron
J
J
Tijssen
P
P
Potworowski
E F
EF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D002460:Cell Line; D018076:DNA, Complementary; D004848:Epithelium / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D013950:Thymus Gland / Q000201:enzymology*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D011505:Protein-Tyrosine Kinases