The detection of adducts in human cervix tissue DNA using 32P-postlabelling: a study of the relationship with smoking history and oral contraceptive use.
32P-Postlabelling was used to measure DNA adducts in the human cervix. Adduct levels were compared with patient smoking histories and contraceptive use. DNA adducts were found in 43 out of 58 samples. The number of adducts ranged from 0.2 to 59.5 adducts/10(8) nucleotides, though no significant difference was found to exist between the number of DNA adducts detected and the smoking history of each patient. In contrast, a significant difference at the 1% probability level was found between the adduct levels obtained from the cervical DNA of smokers who had used oral contraceptives and smokers who did not. Autoradiograms revealed a variety of adduct patterns. Some were found to have a diagonal zone of radioactivity which migrated from the origin of the TLC plate. Other autoradiograms revealed the presence of additional adduct spots located in the upper regions of the TLC plate, whereas others revealed the presence of these adduct spots alone. The origin of the adduct spots located in the upper regions of the TLC plate is unknown.
King
M M
MM
Jack Birch Unit for Environmental Carcinogenesis, Department of Biology, University of York, Heslington, UK.
Hollingsworth
A
A
Cuzick
J
J
Garner
R C
RC
D002584:Cervix Uteri / Q000737:chemistry*; D003276:Contraceptives, Oral / Q000009:adverse effects*; D004247:DNA / Q000032:analysis*; D004249:DNA Damage; D005260:Female; D006801:Humans; D007553:Isotope Labeling; D010761:Phosphorus Radioisotopes; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects*; D002583:Uterine Cervical Neoplasms / Q000139:chemically induced / Q000209:etiology
D003276:Contraceptives, Oral; D010761:Phosphorus Radioisotopes; D004247:DNA