Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids.
Transcription of a putative mitochondrial gene (orf138) has previously been correlated with Ogura cytoplasmic male-sterility (CMS) in rapeseed cybrids. In this paper, studies performed on a Brassica cybrid with a different organization of the orf138 locus confirm this association. We also show that mitochondria isolated from male-sterile rapeseed plants synthesize a polypeptide of 19 kDa, which is absent in fertile revertants. Antibodies against a glutathione S-transferase-ORF138 fusion protein were raised to establish that this 19 kDa polypeptide is the product of orf138. The anti-ORF138 serum was used to demonstrate that the orf138 translation product occurs only in sterile cybrids and co-purifies with the mitochondrial membrane fraction.
Grelon
M
M
Laboratoire de Biologie cellulaire, INRA centre de Versailles, France.
Budar
F
F
Bonhomme
S
S
Pelletier
G
G
D015153:Blotting, Western; D001937:Brassica / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000096:biosynthesis*; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D004591:Electrophoresis, Polyacrylamide Gel; D005298:Fertility / Q000235:genetics; D006824:Hybridization, Genetic; D007425:Intracellular Membranes / Q000737:chemistry; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D010944:Plants / Q000235:genetics*; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial; D008565:Membrane Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D015245:Deoxyribonuclease BamHI; C052380:endodeoxyribonuclease NcoI; D015252:Deoxyribonucleases, Type II Site-Specific