Effect of cell volume on Acridine Orange fluorescence in hepatocytes.
Hepatic proteolysis is inhibited by cell swelling following a variety of experimental manoeuvres, such as reduction of extracellular osmolarity, concentrative uptake of amino acids, or blockade of K+ channels by barium. On the other hand, proteolysis is known to be accomplished by pH-sensitive lysosomal proteases. Accordingly, NH3/NH4+ inhibits proteolysis by intralysosomal alkalinization. The present study has been performed to test for an effect of cell volume on the pH of acidic intracellular compartments, as assessed by Acridine Orange fluorescence at > 520 nm (F > 520). F > 520 is enhanced by NH3/NH4+ (2 and 20 mmol/l respectively), by glutamine (2 mmol/l), by the K(+)-channel blocker barium (10 mmol/l) and by reduction of extracellular osmolarity (by 20 and 80 mosmol/l respectively). The observations point to release of Acridine Orange from acidic cellular compartments, which is indicative of alkalinization of these compartments during cell swelling. This effect may contribute to the regulation of proteolysis.
Völkl
H
H
Institute of Physiology, University of Innsbruck, Austria.
Friedrich
F
F
Häussinger
D
D
Lang
F
F
D000165:Acridine Orange; D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002451:Cell Compartmentation; D048429:Cell Size; D002738:Chloroquine / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism; D005453:Fluorescence; D005973:Glutamine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008985:Monensin / Q000494:pharmacology; D015388:Organelles / Q000378:metabolism*; D009994:Osmolar Concentration
D005973:Glutamine; D001464:Barium; D000641:Ammonia; D002738:Chloroquine; D008985:Monensin; D010450:Endopeptidases; D000165:Acridine Orange