title: Structure and regulation of SDH3, the yeast gene encoding the cytochrome b560 subunit of respiratory complex II.
abstract: Using an expression library, we have isolated yeast genes activated in the presence of the yeast CCAAT box-binding protein HAP2. One of these genes, SDH3, encodes the cytochrome b560 subunit of respiratory complex II. The SDH3 protein contains three potential transmembrane domains and is more than 30% identical to bovine cytochrome b560 and to a mitochondrially encoded protein from Marchantia polymorpha. Disruption of SDH3 shows that this gene is required for growth on non-fermentable carbon sources. Expression of SDH1, SDH3, and SDH4 is activated in the presence of the HAP2 transcriptional activator.
authors.affiliation: Laboratoire de Génétique Moléculaire, Université Paris Sud, Orsay, France.
authors.forename: B
authors.initials: B
authors.lastname: Daignan-Fornier
authors.forename: M
authors.initials: M
authors.lastname: Valens
authors.forename: B D
authors.initials: BD
authors.lastname: Lemire
authors.forename: M
authors.initials: M
authors.lastname: Bolotin-Fukuhara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D023081:CCAAT-Binding Factor; D002417:Cattle; D003573:Cytochrome b Group / Q000096:biosynthesis / Q000235:genetics*; D004251:DNA Transposable Elements; D042963:Electron Transport Complex II; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics*; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013385:Succinate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D023081:CCAAT-Binding Factor; D003573:Cytochrome b Group; D004251:DNA Transposable Elements; D005656:Fungal Proteins; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C025355:cytochrome b560; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D001616:beta-Galactosidase