title: Polygalacturonase isozymes from Botrytis cinerea grown on apple pectin.
abstract: Five isozymes (four acidic and one basic) of polygalacturonase were separated by chromatofocusing from the culture filtrate of Botrytis cinerea grown on apple pectin. The isozymes, designated as Polygalacturonase I to V, have isoelectric points of 9.7, 4.9, 4.6, 3.7, and 2.7, respectively, with Polygalacturonase III exhibiting the highest specific activity. Polygalacturonase I appeared to function as an endo-polygalacturonase while the other four isozymes act as exo-polygalacturonases. The pH optima of the isozymes range from pH 4.5 to 5.5 with Polygalacturonase V being less sensitive to higher pH compared with the rest of the isozymes.
authors.affiliation: Horticultural Crops Quality Laboratory, USDA-ARS, BARC-West, Beltsville, MD 20705.
authors.forename: R
authors.initials: R
authors.lastname: Tobias
authors.forename: W
authors.initials: W
authors.lastname: Conway
authors.forename: C
authors.initials: C
authors.lastname: Sams
mesh_terms: D002855:Chromatography, Thin Layer; D003470:Culture Media; D005638:Fruit; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007526:Isoelectric Point; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000254:growth & development; D010368:Pectins / Q000378:metabolism; D011096:Polygalacturonase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media; D007527:Isoenzymes; D010368:Pectins; D011096:Polygalacturonase; C003181:polygalacturonic acid