Cloning and structure of the human corticotrophin releasing factor-binding protein gene (CRHBP)
The human CRF-binding protein gene has been cloned and mapped to the distal region of chromosome 13 and loci 5q in the mouse and human genomes, respectively. The gene consists of 7 exons and 6 introns. The mature protein has 10 cysteines and 5 tandem disulfide bridges 4 of which are contained within exons 3, 5, 6, and 7. One bridge is shared by exons 3 and 4. The signal peptide and the first 3 amino acids of the mature protein were coded for by an extreme 5' exon. Primer extension analyses revealed the transcriptional initiation site to be located 32 bp downstream from a consensus TATA box. The promoter sequence contained a number of putative promoter elements including an AP-1 site, three ER-half sites, the immunoglobulin enhancer elements NF-kappa B and INF-1, and the liver-specific enhancers LFA1 and LFB1.
Behan
D P
DP
Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, San Diego, California 92037.
Potter
E
E
Lewis
K A
KA
Jenkins
N A
NA
Copeland
N
N
Lowry
P J
PJ
Vale
W W
WW
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
D002352:Carrier Proteins; C067133:corticotropin releasing factor-binding protein; D004247:DNA; D003346:Corticotropin-Releasing Hormone