Determination of the kinetics of degradation of 13-cis-retinoic acid and all-trans-retinoic acid in solution.
The degradations of 13-cis-retinoic acid and all-trans-retinoic acid in an organic solvent were determined with an HPLC assay. The degradation curves at 70, 50 and 37 degrees C all showed autocatalytic characteristics for both isomers. For this kind of complex reaction, the usual method cannot be used to estimate the shelf-lives and half-lives at room temperature. In this work a new method was developed to directly calculate the shelf-lives and half-lives. From this equation the activation energy was found to change as the multiple step reaction progressed.
Tan
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Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
Meltzer
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N
Lindenbaum
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S
D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D015474:Isotretinoin / Q000032:analysis / Q000737:chemistry*; D007700:Kinetics; D008433:Mathematics; D010084:Oxidation-Reduction; D012996:Solutions; D014212:Tretinoin / Q000032:analysis / Q000737:chemistry*
D012996:Solutions; D014212:Tretinoin; D015474:Isotretinoin