title: Establishment of radioimmunoassay for human neutrophil peptides and their increases in plasma and neutrophil in infection.
abstract: Neutrophil peptide, also called defensin, is an antimicrobial peptide localized in azurophil granules in neutrophils. Using a synthetic human neutrophil peptide-1 (HNP-1), we prepared an antiserum against the peptide and established a highly sensitive radioimmunoassay (RIA). This RIA equally recognized HNP-1, -2, and -3 on a molar basis. Analyses of leukocyte and blood extracts obtained from normal individuals and patients with bacterial infection by reverse-phase high-performance liquid chromatography coupled with the RIA showed that the antiserum specifically detected HNP molecules. Concentrations of HNP 1-3 in total in normal human blood and plasma are 13.2 +/- 1.4 (mean +/- SEM) ng/mu 1 and 403.8 +/- 38.9 pg/mu 1, respectively. The values increased in infection and returned to within the normal levels after the recovery of the disorder. Granulocyte colony-stimulating factor (G-CSF) increased the plasma and blood concentrations of HNP 1-3. The RIA established here provides a useful tool to elucidate the pathophysiological significance of HNP and to evaluate the neutrophil functions in infection and inflammation.
authors.affiliation: Department of Internal Medicine, Miyazaki Medical College, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shiomi
authors.forename: M
authors.initials: M
authors.lastname: Nakazato
authors.forename: T
authors.initials: T
authors.lastname: Ihi
authors.forename: K
authors.initials: K
authors.lastname: Kangawa
authors.forename: H
authors.initials: H
authors.lastname: Matsuo
authors.forename: S
authors.initials: S
authors.lastname: Matsukura
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000276:immunology*; D001798:Blood Proteins / Q000032:analysis* / Q000138:chemical synthesis; D002761:Cholangitis / Q000276:immunology; D023082:Defensins; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D016920:Meningitis, Bacterial / Q000276:immunology; D009504:Neutrophils / Q000737:chemistry*; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards; D013966:Thyroiditis / Q000276:immunology; D023084:alpha-Defensins
chemical_list: D001798:Blood Proteins; D023082:Defensins; D023084:alpha-Defensins; C406413:human neutrophil peptide 1