title: The distribution of Tap2 alleles among laboratory rat RT1 haplotypes.
abstract: We are reporting the cDNA sequences of Tap2 from two cima and two cimb rat strains. Comparison of the cDNA sequences shows that these alleles fall into two groups, which we refer to as Tap2-A and Tap2-B. We found that alleles from the Tap2-B group are more closely related to the mouse homologue than are Tap2-A alleles, and among the 48 nucleotides which differ between the Tap2-A and Tap2-B cDNAs, three affect restriction sites. We defined pairs of oligonucleotides which allow amplification of the regions bearing these restriction sites from genomic DNA or cDNA, and this technique has been successful for the genotyping of all of the 56 laboratory strains of Rattus norvegicus tested and for five cell lines tested so far. All 14 known RT1 standard haplotypes were tested, and 7 found to belong to the Tap2-B group, and 7 to Tap2-A. We also found that intron sizes among the alleles of the Tap2-B group fall into two subgroups, providing further insight into the phylogeny of these various haplotypes.
authors.affiliation: Department of Immunology, Babraham Institute, Cambridge, UK.
authors.forename: E
authors.initials: E
authors.lastname: Joly
authors.forename: E V
authors.initials: EV
authors.lastname: Deverson
authors.forename: J W
authors.initials: JW
authors.lastname: Coadwell
authors.forename: E
authors.initials: E
authors.lastname: Günther
authors.forename: J C
authors.initials: JC
authors.lastname: Howard
authors.forename: G W
authors.initials: GW
authors.lastname: Butcher
mesh_terms: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000830:Animals, Laboratory / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000145:classification / Q000235:genetics*; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010802:Phylogeny; D051381:Rats / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D006649:Histocompatibility Antigens; D010455:Peptides; C489516:Tap2 protein, mouse; C489514:Tap2 protein, rat; C038353:histocompatibility antigens RT, rat