title: Distribution of GABAA and GABAB binding sites in the cochlear nucleus of the guinea pig.
abstract: We compared the distribution of GABAA and GABAB binding sites in the cochlear nucleus using quantitative receptor autoradiography with [3H]GABA. To visualize GABAA binding sites, GABAB binding sites were blocked with +/- baclofen. To visualize GABAB binding sites, isoguvacine was used to block GABAA binding sites. GABAA binding sites predominated over GABAB, although there were marked regional differences in the distribution of binding. In the ventral cochlear nucleus, GABAA and GABAB binding sites were concentrated in the peripheral granule cell cap, with low binding levels in the central region. In the dorsal cochlear nucleus, binding was concentrated in the superficial (fusiform and molecular) layers, with a distinct laminar pattern. GABAA binding sites predominated in the fusiform cell layer. The molecular layer contained the highest level of GABAB binding sites in the entire cochlear nucleus. These results suggest that GABAergic inhibition in the cochlear nucleus is mediated both by GABAA and GABAB receptors, particularly in the dorsal cochlear nucleus. However, low levels of binding in areas such as the magnocellular regions of the ventral cochlear nucleus, known to contain abundant GABAergic synapses, suggest heterogeneity of GABA receptors in this auditory nucleus.
authors.affiliation: Kresge Hearing Research Institute, University of Michigan, Ann Arbor 48109.
authors.forename: J M
authors.initials: JM
authors.lastname: Juiz
authors.forename: R L
authors.initials: RL
authors.lastname: Albin
authors.forename: R H
authors.initials: RH
authors.lastname: Helfert
authors.forename: R A
authors.initials: RA
authors.lastname: Altschuler
mesh_terms: D000818:Animals; D001345:Autoradiography; D001418:Baclofen / Q000494:pharmacology; D002525:Cerebellar Cortex / Q000187:drug effects / Q000378:metabolism; D017626:Cochlear Nucleus / Q000187:drug effects / Q000378:metabolism*; D058787:GABA-A Receptor Antagonists; D058788:GABA-B Receptor Antagonists; D006168:Guinea Pigs; D007539:Isonicotinic Acids / Q000494:pharmacology; D011963:Receptors, GABA-A / Q000378:metabolism*; D018080:Receptors, GABA-B / Q000378:metabolism*
chemical_list: D058787:GABA-A Receptor Antagonists; D058788:GABA-B Receptor Antagonists; D007539:Isonicotinic Acids; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D001418:Baclofen; C015300:isoguvacine