title: Protein-free culture of Vero cells: a substrate for replication of human pathogenic viruses.
abstract: A protein-free chemically defined medium designated PFEK-1 was developed for culture of VERO cells on polyvinyl formal (PVF) culture surface without serum or other macromolecular supplements. VERO cells proliferated in PFEK-1 medium on PVF surface to a similar extent as cells in serum-supplemented medium without previous adaptation from serum-containing conditions. The protein-free culture infected with coxsackievirus B4, herpes simplex virus types 1 and 2, measles virus and poliovirus types 1, 2 and 3 developed viral titers comparable to those found in conventionally grown cells. The results demonstrated that VERO cells in protein-free culture provide a sensitive substrate for the production of human pathogenic viruses which are not contaminated by serum or other protein factors usually added to a culture medium.
authors.affiliation: Centre of Hygiene, J. W. Goethe-University, Frankfurt a. M., Fed. Rep. of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: H
authors.initials: H
authors.lastname: Rabenau
authors.forename: J
authors.initials: J
authors.lastname: Rapp
authors.forename: B
authors.initials: B
authors.lastname: Kornhuber
authors.forename: H W
authors.initials: HW
authors.lastname: Doerr
mesh_terms: D000818:Animals; D016895:Culture Media, Serum-Free / Q000032:analysis / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000254:growth & development / Q000502:physiology; D006801:Humans; D008459:Measles virus / Q000254:growth & development / Q000502:physiology; D017955:Poliovirus / Q000254:growth & development / Q000502:physiology; D018139:Simplexvirus / Q000254:growth & development / Q000502:physiology; D014709:Vero Cells / Q000166:cytology / Q000382:microbiology*; D014776:Virus Cultivation / Q000379:methods*; D018406:Virus Physiological Phenomena; D014779:Virus Replication; D014780:Viruses / Q000254:growth & development*
chemical_list: D016895:Culture Media, Serum-Free