title: Immune sensitization augments epithelium-dependent spontaneous tone in guinea pig trachealis.
abstract: We examined epithelial modulation of tracheal smooth muscle (TSM) responsiveness in vitro from guinea pigs receiving active immune sensitization in vivo. Initially, guinea pigs were either ovalbumin sensitized (by aerosol) or sham sensitized with normal saline; TSM responsiveness was assessed isometrically as active tension (AT) after equilibration by electrical field stimulation in vitro. For epithelium-intact (Epi+) tissues, sensitization caused an increase in baseline active spontaneous tone (1.89 +/- 0.20 g AT) vs. sham-sensitized tissues (1.18 +/- 0.28 g AT; P = 0.02). Spontaneous tone in sensitized TSM in which the epithelium was removed (Epi-) (1.01 +/- 0.14 g AT) was substantially less than from Epi+ tissues (P = 0.01) and did not differ from sham-sensitized epithelium-denuded tissues (0.82 +/- 0.24 g AT; P > 0.05). Indomethacin caused a reduction in spontaneous tone to comparable magnitude for all treatment paradigms. Immune sensitization caused physiological reduction in the ability to relax in response to isoproterenol; the concentration of isoproterenol eliciting 50% relaxation of spontaneous tone was 7.10 +/- 0.13 (-log M) for TSM from sensitized guinea pigs compared with 8.20 +/- 0.27 (-log M) for sham-sensitized tissues (P = 0.006). However, after precontraction with exogenous acetylcholine, relaxation caused by isoproterenol was not affected by either indomethacin or epithelial removal. Muscarinic responsiveness to acetylcholine was augmented by immune sensitization; however, the increase in response to acetylcholine was attenuated by epithelium removal or cyclooxygenase blockade.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, University of Chicago, Illinois 60637.
authors.forename: I M
authors.initials: IM
authors.lastname: Ndukwu
authors.forename: J
authors.initials: J
authors.lastname: Solway
authors.forename: K
authors.initials: K
authors.lastname: Arbetter
authors.forename: K
authors.initials: K
authors.lastname: Uzendoski
authors.forename: A R
authors.initials: AR
authors.lastname: Leff
authors.forename: R W
authors.initials: RW
authors.lastname: Mitchell
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000336:Aerosols; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D006168:Guinea Pigs; D007114:Immunization; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009129:Muscle Tonus / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D011433:Propranolol / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects / Q000276:immunology / Q000502:physiology*
chemical_list: D000336:Aerosols; D013779:Tetrodotoxin; D001285:Atropine; D010047:Ovalbumin; D011433:Propranolol; D007545:Isoproterenol; D000109:Acetylcholine; D007213:Indomethacin