Human islet isolation--a prospective randomized comparison of pancreatic vascular perfusion with hyperosmolar citrate or University of Wisconsin solution.
University of Wisconsin solution has become the most commonly used vascular perfusate during multiorgan donation world-wide. In the UK however, hyperosmolar citrate remains in common use. The purpose of this prospective randomized study was to compare the effect of systemic perfusion with UW or HOC on subsequent islet yield and purification for pancreata with short cold ischemic times. Seven pancreata were randomized to each group, with the donor age, pancreas weight, and period of cold ischemia being similar in both. Perfusion with UW was shown to inhibit collagenase digestion, and a higher concentration of this enzyme was needed to achieve comparable numbers of islets with good separation of exocrine and islet tissue after a similar period of digestion. There were no differences in the number, size, purity, or viability of islets between the two groups. In conclusion, UW solution offers no benefits over HOC for pancreata with short cold ischemic times, and because of its expense and need to use greater amounts of collagenase enzyme, we continue to use HOC.
Robertson
G S
GS
Department of Surgery, Leicester Royal Infirmary, United Kingdom.
Chadwick
D
D
Thirdborough
S
S
Swift
S
S
Davies
J
J
James
R
R
Bell
P R
PR
London
N J
NJ
D000241:Adenosine; D000493:Allopurinol; D002470:Cell Survival; D002951:Citrates / Q000737:chemistry; D019343:Citric Acid; D003080:Cold Temperature / Q000009:adverse effects; D017364:Collagenases / Q000378:metabolism; D005978:Glutathione; D006801:Humans; D007328:Insulin; D007511:Ischemia / Q000209:etiology; D007515:Islets of Langerhans / Q000166:cytology; D019188:Organ Preservation Solutions; D009994:Osmolar Concentration; D010179:Pancreas / Q000098:blood supply*; D010477:Perfusion; D011446:Prospective Studies; D011887:Raffinose; D011897:Random Allocation; D014019:Tissue Donors
D002951:Citrates; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D019343:Citric Acid; D000493:Allopurinol; D017364:Collagenases; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose