Localization of myosin in normal human liver hepatocytes using immunohistochemical amplification methods.
Different immunohistochemical amplification systems were used to visualise myosin in normal human hepatocytes. With biotin-streptavidin-peroxidase and immunogold silver staining, myosin was distributed along the plasma membranes and at the bile canaliculi. With biotin-streptavidin-rhodamine, myosin was mainly found at the bile canaliculi level, however with confocal microscopy, the plasma membrane fluorescent staining was more apparent. The staining pattern of myosin appeared to be similar to that of actin in normal human hepatocytes.
Benkoël
L
L
Laboratoire d'Anatomie Pathologique et de Cytochimie Hépatique, Faculté de Médecine, Marseille, France.
Brisse
J
J
Benoliel
A M
AM
Chamlian
A
A
D000199:Actins / Q000032:analysis; D001426:Bacterial Proteins; D001653:Bile Ducts, Intrahepatic / Q000737:chemistry; D001710:Biotin; D002462:Cell Membrane / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry*; D008853:Microscopy / Q000379:methods; D009218:Myosins / Q000032:analysis*; D012235:Rhodamines; D016622:Silver Staining; D019809:Streptavidin
D000199:Actins; D001426:Bacterial Proteins; D012235:Rhodamines; D001710:Biotin; D019809:Streptavidin; D009218:Myosins