Distribution of cytopathogenic and noncytopathogenic bovine virus diarrhea virus in tissues from a calf with experimentally induced mucosal disease using antigenic and genetic markers.
A comparative analysis of the distribution of cytopathogenic (cp) and noncytopathogenic (ncp) bovine virus diarrhea disease (BVD) virus in tissues from a calf with experimentally induced mucosal disease was performed using immunohistology and polymerase chain reaction after reverse transcription (RT-PCR) of viral RNA. For immunohistology, an antigenic marker on the superinfecting cp BVD virus defined by a monoclonal antibody (mab) was used, and overall presence of antigen was assessed with a pestivirus specific mab. The primers selected for RT-PCR detected the genomic insertion in the p125 region of the superinfecting cp BVD virus. Both methods gave consistent results.
Greiser-Wilke
I
I
Institute of Virology, Hannover Veterinary School, Federal Republic of Germany.
Liebler
E
E
Haas
L
L
Liess
B
B
Pohlenz
J
J
Moennig
V
V
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D005819:Genetic Markers; D012367:RNA, Viral