[Preliminary experiments on the mode of action of oxalysine on Candida albicans and C. parapsilosis].
Oxalysine, [formula: see text] is a novel anti-fungal antibiotic isolated from Streptomyces roseoviridofuscus n. sp.. MIC values 0.8-3.1 micrograms/ml suggested a good activity of oxalysine against Candida parapsilosis when compared to Amphotericin-B and 5-Fluorocytosine. The MIC for Trichophyton rubrum and Microsporeum gypseum range from 1.56-3.13 micrograms/ml. Transportation of radioactivity labelled (3H)-Lysine compete with oxalysine was used to characterize Lysine permease in Candida albicans. The results showed that oxalysine 10-folds did not reduce the rate of (3H)-Lysine uptake. This showed low affinity of lysine permease to oxalysine in Candida albicans. When the leakage of U V 260 nm-absorbing materials was used as the evidence of permeability of the cell membrane. Oxalysine was not observed to cause leakage of cellular constituents. The result showed that the action of oxalysine is not on the cell membrane of the microorganism. The incorporations of (14C)-Methione and (14C)-Adenine were used as a method of measuring the antimicrobial action of the drug oxalysine is on protein and/or nucleic acid synthesis. It was found that oxalysine 0.4 mmol/L did not significantly inhibit their incorporations into protein and/or DNA, but strongly inhibit the incorporation of (14C)-Adenine into RNA.
Zhang
H
H
Shanghai Institute of Materia Madica, Chinese Academy of Sciences.
Gu
X
X
Wang
Q
Q
Becker
J M
JM
D000600:Amino Acids, Dicarboxylic / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*
D000600:Amino Acids, Dicarboxylic; D000935:Antifungal Agents; C027136:4-oxalysine