title: 99mTc labeling white blood cells with a simple technique: clinical application.
abstract: Several radionuclides and different methods have been employed as cellular labels to study inflammatory sites in man. Here we present the results obtained with white blood cells (WBC) labeled with 99mTc using a simple and low cost new technique (SnTec). WBC were incubated with 12 micrograms/ml of stannous chloride for 10 min at room temperature. Then 99mTc was added. After 10 min, the 99mTc-labeled WBC were washed and injected into the patient. Comparison studies with 99mTc-labeled WBC using the HMPAO technique were carried out in patients with suspected osteomyelitis. Since the results are similar with both methods, we suggest the use of SnTec to label WBC, in cases of inflammatory diseases.
authors.affiliation: Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Brasil.
authors.forename: B
authors.initials: B
authors.lastname: Gutfilen
authors.forename: M P
authors.initials: MP
authors.lastname: Pellini
authors.forename: J
authors.initials: J
authors.lastname: de Roure e Neder
authors.forename: J L
authors.initials: JL
authors.lastname: de Amarante Júnior
authors.forename: M G
authors.initials: MG
authors.lastname: Evangelista
authors.forename: S R
authors.initials: SR
authors.lastname: Fernandes
authors.forename: M
authors.initials: M
authors.lastname: Bernardo-Filho
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D007962:Leukocytes; D008297:Male; D008875:Middle Aged; D015609:Organotechnetium Compounds; D010019:Osteomyelitis / Q000000981:diagnostic imaging*; D010091:Oximes; D011877:Radionuclide Imaging; D013667:Technetium; D019690:Technetium Tc 99m Exametazime
chemical_list: D015609:Organotechnetium Compounds; D010091:Oximes; D019690:Technetium Tc 99m Exametazime; D013667:Technetium