title: Cryopreserved saphenous vein allografts for below-knee lower extremity revascularization.
abstract: OBJECTIVE
  Cryopreserved saphenous vein allografts have been offered as an alternative conduit for bypass in ischemic limbs. The authors examined the efficacy of this conduit for arterial bypass to the distal popliteal and tibial arteries in patients in whom autogenous vein was not available.
  
  
  BACKGROUND
  Previous experience with arterial and venous allografts has been unsatisfactory because of aneurysmal degeneration and poor patency. Endothelial loss and host rejection have been suggested as mechanisms of graft failure. Cryopreservation by modern techniques with rate controlled freezing, dimethyl sulfoxide (DMSO), and other cryopreservants, has addressed these issues and rekindled interest in vein allografts.
  
  
  METHODS
  Over a period of more than 5 years, 115 cryopreserved vein allografts were implanted in 87 limbs to the distal popliteal (14) or tibial (101) arteries. The indication for surgery was rest pain in 56 procedures (49%), gangrene in 36 (31%), claudication in 21 (18%), and replacement of aneurysmal allografts in 2. Follow-up was 1 to 61 months (mean 25 months).
  
  
  RESULTS
  There was no significant difference in patency related to site of proximal or distal anastomosis, patency of runoff vessels, use of anticoagulation, age, sex, diabetes, hypertension, smoking, indication, source of graft, or use of multiple segments. Revision was required in six grafts for aneurysmal dilatation. Histologic examination of explanted sections of allografts showed no immune response, and immunosuppressive drugs were not used.
  
  
  CONCLUSIONS
  Although limb salvage has been satisfactory, long-term patency rates for cryopreserved vein allografts are poor when compared with autogenous vein. The cost of cryopreserved allografts far exceeds that of prosthetic grafts, for which comparable and superior results have been reported. Use of cryopreserved vein allografts should be reserved for situations in which adequate lengths of autogenous vein do not exist and the risk of infection of prosthetic grafts is high.
authors.affiliation: Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
authors.forename: R S
authors.initials: RS
authors.lastname: Martin
authors.forename: W H
authors.initials: WH
authors.lastname: Edwards
authors.forename: J L
authors.initials: JL
authors.lastname: Mulherin
authors.forename: W H
authors.initials: WH
authors.lastname: Edwards
authors.forename: J M
authors.initials: JM
authors.lastname: Jenkins
authors.forename: S J
authors.initials: SJ
authors.lastname: Hoff
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000714:Anastomosis, Surgical; D015925:Cryopreservation; D005260:Female; D005268:Femoral Vein / Q000601:surgery; D005500:Follow-Up Studies; D006801:Humans; D007717:Knee; D007866:Leg / Q000098:blood supply* / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D011152:Popliteal Vein / Q000601:surgery; D011183:Postoperative Complications / Q000453:epidemiology; D012501:Saphenous Vein / Q000637:transplantation*; D016896:Treatment Outcome; D014654:Vascular Patency