Differential transcription, without replication, of non-structural and structural genes of human parvovirus B19 in the UT7/EPO cell as demonstrated by in situ hybridization.
Erythroid progenitor cells are the main target for B19 parvovirus infection. The UT7 cell line demonstrates a marked erythroid differentiation on induction by erythropoietin (EPO) (UT7/EPO cells) and therefore appears to be a potential target for B19 parvovirus. We aimed to evaluate the presence and localization of B19 nucleic acids in UT7/EPO cells by in situ hybridization. Three digoxigenin-labelled probes were used: two recognized specifically the non-structural region of the B19 genome and one probe was structural region-specific. In our experiment UT7/EPO cells were not permissive to B19 infection. Transcription led to nonstructural and structural gene transcripts without DNA replication or capsid protein synthesis.
Leruez
M
M
Hopital Saint-Louis, Service de Microbiologie, Paris, France.
Pallier
C
C
Vassias
I
I
Elouet
J F
JF
Romeo
P
P
Morinet
F
F
D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016732:Parvovirus B19, Human / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
D017931:DNA Primers; D014764:Viral Proteins; D015678:Viral Structural Proteins