Influence of DNA conformation on radiation-induced single-strand breaks.
It is usually assumed that sparsely ionizing radiation produces randomly distributed DNA breakages. This seems to be supported by the finding that in some DNA fragments single-strand scissions occur uniformly at all nucleotide sites, regardless of sequence. We performed experiments on two DNA fragments of about 300 bp having different conformation to test whether radiation-induced single-strand breakage is dependent on DNA conformation. Breakage analysis was carried out by denaturing polyacrylamide gel electrophoresis, which allows determination of the broken site at single nucleotide resolution. We found uniform cutting patterns in B-form regions. On the contrary, X- or gamma-irradiation of curved fragments of kinetoplast DNA showed that the distribution of single-strand breaks was not uniform along the fragment, as the cleavage pattern was modulated in phase with the runs of A-T pairs. This modulation likely reflected the reduced accessibility of the sites which on hydroxyl-radical attack give rise to strand breaks. The cleavage pattern was phased with the runs of A-T pairs. Moreover, the overall yield of strand breaks was considerably lower in curved DNA fragments than in those with extended straight regions. The conformation effect found here indicates that the cleavage pattern reflects the fine structural features of DNA.
Barone
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Laboratorio di Fisica, Istituto Superiore di Sanità, Roma, Italy.
Belli
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Mazzei
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D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000528:radiation effects; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation
D004277:DNA, Single-Stranded; D004247:DNA