The preparation of fibrinogen concentrate for use as fibrin glue by four different methods.
Fibrinogen concentrates for use as fibrin glue were prepared by modification of a cryoprecipitate method. The goals were the optimization of a method for different centrifuges and anticoagulants and the assay of factors not previously analyzed. Following a -70 degrees C freeze and a 4 degrees C thaw, CPDA-1 and ACD plasma were centrifuged at 6500 x g for 5 minutes or, alternatively, at 5000 x g for 7 minutes. The supernatant plasma was expressed to a final volume of 15.5 +/- 3 mL, and concentrates were stored at -30 degrees C. Preconcentration and postconcentration samples were analyzed for fibrinogen, fibronectin, factor XIII, and plasminogen content. Fibrinogen in CPDA-1 plasma was significantly higher than that in ACD plasma both before and after concentration at both centrifugation speeds. Fibronectin, factor XIII, and plasminogen concentrations were not significantly affected by centrifugation speed or the type of anticoagulant used. Fibronectin and plasminogen concentrations were significantly increased in components that were held for 5 to 6 days, as compared to those held for 0 to 1 day before freezing. Storage for up to 20 days in CPDA-1 and up to 5 months in ACD did not affect analyte concentration. It is concluded that ACD plasma centrifuged at 5000 x g yields a significantly low concentration of fibrinogen, while CPDA-1 plasma centrifuged at 6500 x g yields the highest amount. Acceptable yields were obtained from centrifugation of ACD plasma at 6500 x g and of CPDA-1 plasma at 5000 x g for use as fibrin glue.
DePalma
L
L
Department of Laboratory Medicine, Children's National Medical Center, Washington, D.C.
Criss
V R
VR
Luban
N L
NL
D000225:Adenine; D000925:Anticoagulants; D001793:Blood Preservation; D002498:Centrifugation / Q000379:methods*; D011232:Chemical Precipitation; D002951:Citrates; D019343:Citric Acid; D015925:Cryopreservation; D005169:Factor VIII / Q000032:analysis; D015718:Fibrin Tissue Adhesive; D005340:Fibrinogen / Q000032:analysis / Q000302:isolation & purification*; D005353:Fibronectins / Q000032:analysis; D005947:Glucose / Q000031:analogs & derivatives; D006801:Humans; D010710:Phosphates; D010958:Plasminogen / Q000032:analysis; D013997:Time Factors
D000925:Anticoagulants; C034876:CPDA solutions; D002951:Citrates; D015718:Fibrin Tissue Adhesive; D005353:Fibronectins; D010710:Phosphates; C002113:acid citrate dextrose; D019343:Citric Acid; D005169:Factor VIII; D005340:Fibrinogen; D010958:Plasminogen; D005947:Glucose; D000225:Adenine