title: Identification of a novel sugar, 4-amino-4,6-dideoxy-2-O-methylmannose in the lipopolysaccharide of Vibrio cholerae O1 serotype Ogawa.
abstract: A novel sugar in the lipopolysaccharide of Vibrio cholerae O1 serotype Ogawa has been identified. The sugar was liberated from the lipopolysaccharide when hydrolyzed in 10 M HCl at 90 degrees C for 15 min. The sugar was purified and identified as 4-amino-4,6-dideoxy-2-O-methylmannose (2-O-methylperosamine). Since it was found only in the lipopolysaccharide of Vibrio cholerae O1 serotype Ogawa, it seems that the sugar is one of the specific constituents determining Ogawa serotype specificity.
authors.affiliation: Department of Bacteriology, School of Medicine, Juntendo University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Higuchi
authors.forename: M
authors.initials: M
authors.lastname: Hirobe
authors.forename: K
authors.initials: K
authors.lastname: Hiramatsu
authors.forename: T
authors.initials: T
authors.lastname: Yokota
mesh_terms: D002236:Carbohydrate Conformation; D002852:Chromatography, Ion Exchange; D006595:Hexosamines / Q000032:analysis* / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry; D015394:Molecular Structure; D012703:Serotyping; D014734:Vibrio cholerae / Q000737:chemistry* / Q000145:classification / Q000276:immunology
chemical_list: C087384:4-amino-4,6-dideoxy-2-O-methylmannose; D006595:Hexosamines; D008070:Lipopolysaccharides; D008358:Mannose