title: Cell Ca2+ response to luminal vasopressin in cortical collecting tubule principal cells.
abstract: Although vasopressin V1 receptors have been shown to exist in both luminal and basolateral membranes of rabbit cortical collecting duct (CCD), exact cell types having V1 receptors remain unestablished. To identify the distribution of V1 receptor by cytoplasmic Ca2+ response, we utilized the confocal imaging system in the microperfused rabbit CCD. Basolateral application of arginine vasopressin (AVP) increased [Ca2+]i mainly in one group of cells which were not stained by fluorescein-isothiocyanate-conjugated peanut agglutinin. Luminal application of AVP increased [Ca2+]i in the same cells which responded to basolateral AVP. These findings provide evidence that V1 receptors, as defined by the [Ca2+]i response, exist in both luminal and basolateral membranes of the rabbit principal cell.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Jichi Medical School, Tochigi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Yoshitomi
authors.forename: M
authors.initials: M
authors.lastname: Imai
authors.forename: K
authors.initials: K
authors.lastname: Kurokawa
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001485:Basement Membrane / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D007672:Kidney Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D037102:Lectins; D008856:Microscopy, Fluorescence; D019887:Peanut Agglutinin; D010477:Perfusion; D011817:Rabbits; D017483:Receptors, Vasopressin / Q000378:metabolism
chemical_list: D037102:Lectins; D019887:Peanut Agglutinin; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D016650:Fluorescein-5-isothiocyanate; D002118:Calcium