Stimulation of aldosterone production by hemin in calf adrenal glomerulosa cell cultures.
Aldosterone production from 11-deoxycorticosterone was stimulated by hemin in primary cultures and homogenates of calf adrenal zona glomerulosa, in a time- and dose-dependent fashion. The ferrochelatase inhibitor 3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-trimethylpyridine (DDC) blocked the stimulation of aldosterone mediated by adrenocorticotropin (ACTH). Addition of hemin after treatment with DDC partially restored ACTH action. These results suggest that hemin may play a role in regulation of aldosterone production.
Cozza
E N
EN
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Vila
M C
MC
Gomez-Sanchez
C E
CE
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000096:biosynthesis*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003997:Dicarbethoxydihydrocollidine / Q000494:pharmacology; D005260:Female; D005294:Ferrochelatase / Q000037:antagonists & inhibitors; D006427:Hemin / Q000494:pharmacology*; D007700:Kinetics; D015384:Zona Glomerulosa / Q000187:drug effects / Q000378:metabolism*
D000450:Aldosterone; D003997:Dicarbethoxydihydrocollidine; D006427:Hemin; D000324:Adrenocorticotropic Hormone; D005294:Ferrochelatase