title: A new genomic species in Borrelia burgdorferi sensu lato isolated from Japanese ticks.
abstract: Five Borrelia strains (Ika2, HO14, Cow611C, 0612 and F63B) isolated from Ixodes ovatus ticks in Japan were analysed by DNA-DNA hybridization experiments, ribotyping, pulsed-field gel electrophoresis and protein electrophoresis. DNA relatedness set these strains in a new genomic species within the Borrelia burgdorferi complex; this species appears to be restricted to Japan and could be non-pathogenic for humans. The ribotype and pulsotype of strain Ika2 were atypical of the new genomic species.
authors.affiliation: Unité de Bactériologie moléculaire et médicale, Institut Pasteur, Paris.
authors.forename: D
authors.initials: D
authors.lastname: Postic
authors.forename: J
authors.initials: J
authors.lastname: Belfaiza
authors.forename: E
authors.initials: E
authors.lastname: Isogai
authors.forename: I
authors.initials: I
authors.lastname: Saint Girons
authors.forename: P A
authors.initials: PA
authors.lastname: Grimont
authors.forename: G
authors.initials: G
authors.lastname: Baranton
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007564:Japan; D008193:Lyme Disease / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics; D015183:Restriction Mapping; D013987:Ticks / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012335:RNA, Ribosomal