A simplified protocol for isolation and characterization of ssM13 DNA templates for use in dideoxy sequencing.
Meeker
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Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Li
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YK
Shortle
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Stites
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D017104:Bacteriophage M13 / Q000235:genetics*; D002498:Centrifugation; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification*; D006358:Hot Temperature; D017422:Sequence Analysis, DNA; D013698:Templates, Genetic
D004277:DNA, Single-Stranded; D004279:DNA, Viral