Identification of four genes involved in the lysogenic pathway of the Salmonella newington bacterial virus epsilon 34.
A structure/function study has been initiated for the epsilon 34 bacteriophage proteins involved in lysogeny in Salmonella newington. Hydroxylamine and nitrosoguanidine mutagenesis of a wild type epsilon 34 phage was used to generate clear plaque variants. Complementation analysis was used to define four genes involved in the phage lysogenic pathway. A relative mapping order has been established. In addition, a virulent mutant, epsilon 34vir82, which defines a repressor binding site has been isolated.
Villafane
R
R
Department of Microbiology, University of Tennessee, Knoxville.
Black
J
J
D005814:Genes, Viral; D005816:Genetic Complementation Test; D014644:Genetic Variation; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D008242:Lysogeny / Q000235:genetics*; D016296:Mutagenesis; D009604:Nitrosoguanidines / Q000494:pharmacology; D011995:Recombination, Genetic; D012475:Salmonella / Q000235:genetics; D012483:Salmonella Phages / Q000187:drug effects / Q000235:genetics*; D010948:Viral Plaque Assay
D006898:Hydroxylamines; D009604:Nitrosoguanidines; D019811:Hydroxylamine