[Analysis and regulation of interferon-gamma production by peripheral blood lymphocytes from patients with bronchial asthma].
As with normal lymphocytes, small amounts of interferon-gamma (IFN-gamma) were spontaneously produced by lymphocytes from patients with bronchial asthma, and this bronchial asthma, and this production was markedly enhanced by concanavalin A (Con A) stimulation. Distinct from normal lymphocytes, however, IFN-gamma synthesis from patients' lymphocytes decreased in a dose dependent manner on stimulation with Dermatophagoides farinae (Df) antigen. IFN-gamma-producing cells are defined as being of the CD4+ 45RO+ T cell subset, which failed to produce IFN-gamma in Df-stimulated patients' lymphocytes. As the decreased production of IFN-gamma by antigenic stimulation was exclusively induced by Df antigen, but not by ovalbumin (OVA) or Japanese cedar (JC) antigen, it is concluded that Df antigen specifically suppressed IFN-gamma production in patients' lymphocytes. The IFN-gamma synthesis from normal lymphocytes was suppressed by the addition of IL-4 in a dose dependent manner. Moreover, anti-IL-4 antibody dose dependently enhanced IFN-gamma production from Df-stimulated patients' lymphocytes but not from unstimulated lymphocytes. These results indicate that IFN-gamma production by patients' lymphocytes is suppressed by the endogenous IL-4. Reduced IFN-gamma production in patients' lymphocytes on stimulation with Df antigen may lead to the overproduction of IgE in vivo.
Kou
K
K
Department of Pediatrics, Saitama Medical School.
Kawano
Y
Y
Yoshizawa
I
I
Noma
T
T
D000293:Adolescent; D000328:Adult; D000818:Animals; D001249:Asthma / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000494:pharmacology / Q000502:physiology; D008297:Male; D008925:Mites / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma