Molecular cloning of mannose-binding lectins from Clivia miniata.
Screening of a cDNA library constructed from total RNA isolated from young developing ovaries of Clivia miniata Regel with the amaryllis lectin cDNA clone resulted in the isolation of four different isolectin clones which clearly differ from each other in their nucleotide sequences and hence also in their deduced amino acid sequences. Apparently the lectin is translated from an mRNA of ca. 800 nucleotides encoding a precursor polypeptide of 163 amino acids. Northern blot analysis of total RNA isolated from different tissues of Clivia miniata has shown that the lectin is expressed in most plant tissues with very high lectin mRNA concentrations in the ovary and the seed endosperm.
Van Damme
E J
EJ
Laboratory for Phytopathology and Plant Protection, Catholic University of Leuven, Belgium.
Smeets
K
K
Van Leuven
F
F
Peumans
W J
WJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D037102:Lectins / Q000235:genetics* / Q000302:isolation & purification; D008358:Mannose / Q000378:metabolism*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D037102:Lectins; D037121:Plant Lectins; D012333:RNA, Messenger; D008358:Mannose