Determination of free myo-inositol in milk and infant formula by high-performance liquid chromatography.
A high-performance liquid chromatographic method is described for the determination of supplemental myo-inositol in infant formulas based on cows' milk. The technique incorporates pre-column derivatization with phenylisocyanate followed by reversed-phase gradient chromatography and ultraviolet detection. The protocol was also applied to a survey of free inositol at endogenous levels in the milk of cows, goats and humans. Nutritional preparations containing inositol at pharmacological levels are also amenable to a simplified isocratic analysis.
Indyk
H E
HE
Anchor Products, Waitoa, New Zealand.
Woollard
D C
DC
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006041:Goats; D006801:Humans; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D007294:Inositol / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry
D007294:Inositol