Sequence of the human dopachrome tautomerase-encoding TRP-2 cDNA.
We have isolated cDNAs for the human TRP-2 gene which represents a third member of the tyrosinase-related gene family and encodes the dopachrome tautomerase activity that functions in the synthesis of melanin pigment. The human TRP-2 protein has 83% identity/90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, two copper-binding domains and a C-terminal membrane-spanning region. Northern blot analysis reveals that TRP-2 is expressed at high levels in human melanoma cells.
Cassady
J L
JL
Centre for Molecular Biology and Biotechnology, University of Queensland, Brisbane, Australia.
Sturm
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006801:Humans; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000737:chemistry / Q000235:genetics*; D008545:Melanoma / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase