title: A low-temperature-responsive translation elongation factor 1 alpha from barley (Hordeum vulgare L.).
abstract: A cDNA clone (pBLT63) encoding a protein synthesis elongation factor 1 alpha (EF-1 alpha) was isolated from a low-temperature winter barley shoot meristem library by differential screening. The nucleotide sequence of the coding region of the low-temperature-induced barley gene shows very high homology with two EF-1 alpha plant genes from tomato and Arabidopsis. The barley genome contains an EF-1 alpha gene family situated on the short arm of chromosome 2 and the long arm of chromosome 5.
authors.affiliation: Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Dunn
authors.forename: A
authors.initials: A
authors.lastname: Morris
authors.forename: P L
authors.initials: PL
authors.lastname: Jack
authors.forename: M A
authors.initials: MA
authors.lastname: Hughes
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D017385:Sequence Homology; D013696:Temperature
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010940:Plant Proteins; D004247:DNA