Difference in sero-diagnostic values among KL-6-associated mucins classified as cluster 9.
KL-6 classified as Cluster 9 (MUC-I) is a circulating high-molecular-weight mucin-like molecule. Serum level of KL-6 was measured by a sandwich assay using KL-6 antibody as not only a catcher but also as a tracer. We established 2 additional monoclonal antibodies (MAbs), LISA 101 and EH-123, reacting with KL-6 epitopes different from the epitope recognized by KL-6 antibody. The KL-6-associated mucins detected by the sandwich assay using LISA 101 or EH-123 antibody as a catcher and KL-6 antibody as a tracer were designated as LISA 1-6 and CAM 123-6 respectively. The diagnostic values as the serum markers of KL-6, LISA 1-6 and CAM 123-6 were evaluated measuring their levels in the same serum from healthy individuals and from patients with pulmonary, pancreatic and breast adenocarcinomas. KL-6 was increased abnormally at high rates of more than 50% in pancreatic cancer and in benign lung diseases, LISA 1-6 only in pancreatic cancer, and CAM 123-6 only in pulmonary adenocarcinoma. In benign lung diseases, however, LISA 1-6 and CAM 123-6 were increased abnormally at the rates of only 5.3% and 0% respectively. These observations clearly indicate that LISA 1-6 and CAM 123-6 constitute a part of KL-6, but that they are superior to KL-6 as tumor markers for pancreatic cancer and for pulmonary adenocarcinoma respectively, because of their much lower false-positive rates.
Kohno
N
N
Second Department of Internal Medicine, Ehime University School of Medicine, Japan.
Inoue
Y
Y
Hamada
H
H
Fujioka
S
S
Fujino
S
S
Yokoyama
A
A
Hiwada
K
K
Ueda
N
N
Akiyama
M
M
D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis*; D000941:Antigens / Q000097:blood*; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis*; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D008171:Lung Diseases / Q000097:blood; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis*; D018396:Mucin-1; D009077:Mucins / Q000097:blood*; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis*; D012016:Reference Values
D000941:Antigens; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C060192:MUC1 protein, human; D018396:Mucin-1; D009077:Mucins