Cloning and chromosomal organization of a gene encoding a putative amino-acid permease from Saccharomyces cerevisiae.
A new member of the yeast amino acid (aa) permease gene family has been cloned, mapped and sequenced. The sequence of the PAP1 (putative aa permease 1) gene contains an open reading frame of 566 aa corresponding to a polypeptide with a calculated M(r) of 62,704. Its hydropathy profile suggests the presence of 13 membrane-spanning regions and a charged N-terminal domain. It does not resemble hydrophobic signal sequences found in secreted proteins. Hence, PAP1 encodes a protein with characteristics typical of integral membrane proteins translocating ligands across cellular membranes. Sequence comparisons indicate strong homology to the five known aa permeases of Saccharomyces cerevisiae and to an aa transporter in Trichoderma harzianum. Primer extension analysis revealed one major and one minor transcription start point located 121 and 125 nucleotides upstream from the ATG start codon, corresponding to a 2.1-kb transcript. PAP1 was mapped in a contig of three known (DBF4, TPI and HEM12), but so far unlinked, genes on chromosome IV.
Mai
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Institut für Biochemie der Ludwig-Maximilians-Universität München, Martinsried, Germany.
Lipp
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D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics; D015825:Chromosomes, Fungal / Q000648:ultrastructure; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D000073718:Pancreatitis-Associated Proteins; D010802:Phylogeny; D011062:Polynucleotide Adenylyltransferase; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013728:Terminator Regions, Genetic; D014242:Trichoderma / Q000235:genetics
D026905:Amino Acid Transport Systems; C429560:BAP3 protein, S cerevisiae; D002352:Carrier Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D029701:Saccharomyces cerevisiae Proteins; C429559:PAP1 protein, S cerevisiae; D011062:Polynucleotide Adenylyltransferase