title: Novel immunohistochemical localization of 28,000 molecular-weight (Mr) calcium binding protein (calbindin-D28k) in enterochromaffin cells of the human appendix and neuroendocrine tumors (carcinoids and small-cell carcinomas) of the midgut and foregut.
abstract: Calbindin-D28k is a highly conserved 28,000 (dalton) molecular-weight (Mr) calcium binding protein with broad tissue distribution, yet cell-type-specific expression predominantly in subpopulations of central and peripheral nervous system neurons, distal tubular cells of the kidney, and enteric neuroendocrine cells. A polyclonal antiserum against rat renal calbindin-D28k and a monoclonal antibody to calbindin-D28k purified from chicken intestine (clone CL-300) were used for immunohistochemical evaluation of formalin-fixed, paraffin-embedded tissues from multiple areas of the human small and large intestines and 93 primary neoplasms of the gastrointestinal tract (foregut, midgut, and hindgut derivatives) and the lung (foregut derivative). Calbindin-D28k immunostaining was obtained in a minority of enterochromaffin (neuroendocrine) cells, predominantly of the appendix and small intestine, as well as in autonomic neurons of the neural plexuses. Focal cytoplasmic Golgi-type staining was obtained with monoclonal antibody CL-300 in the appendiceal surface epithelium and dendritic macrophages confined to the appendiceal lymphoid follicles. Epithelial progenitor cells in enteric crypts and absorptive, goblet, and Paneth cells were calbindin-D28k negative, while no immunoreactivity was demonstrated in the mucosae of the colon and rectum. Calbindin-D28k staining was consistently detected in subpopulations of neuroendocrine phenotypes in midgut (appendiceal/ileal) and foregut (bronchial) carcinoids and small-cell carcinomas, but was absent in adenocarcinomas, squamous cell carcinomas, leiomyomas/leiomyosarcomas, schwannomas, and lymphomas. Our observations suggest that calbindin-D28k is a novel adjuvant neuroendocrine marker that is potentially useful in diagnostic tumor immunohistochemistry.
authors.affiliation: Neuropathology Laboratory, Hahnemann University, Philadelphia, Pa 19102-1192.
authors.forename: C D
authors.initials: CD
authors.lastname: Katsetos
authors.forename: M M
authors.initials: MM
authors.lastname: Jami
authors.forename: L
authors.initials: L
authors.lastname: Krishna
authors.forename: R
authors.initials: R
authors.lastname: Jackson
authors.forename: A S
authors.initials: AS
authors.lastname: Patchefsky
authors.forename: H S
authors.initials: HS
authors.lastname: Cooper
mesh_terms: D000328:Adult; D064092:Calbindin 1; D064026:Calbindins; D002276:Carcinoid Tumor / Q000737:chemistry*; D018288:Carcinoma, Small Cell / Q000737:chemistry*; D004064:Digestive System / Q000737:chemistry; D004759:Enterochromaffin Cells / Q000737:chemistry*; D005770:Gastrointestinal Neoplasms / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry*; D008970:Molecular Weight; D064030:S100 Calcium Binding Protein G / Q000032:analysis*
chemical_list: C576498:CALB1 protein, human; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G