Quantitative approach to the immunohistochemical analysis of p53 antioncogene and CD30 antigen in Hodgkin' disease.
In this paper a multimarker immunohistochemical technique is described in detail which allows the quantitative evaluation in situ of cells expressing abnormal accumulation of p53 phosphoprotein together with the CD30 antigen, a well known marker of Hodgkin's and Reed-Sternberg cells. The method is useful to define quantitatively the proportion of phenotypically abnormal cells in the complex microenvironment of Hodgkin's lesions, and to compare the phenotypical derangement of p53 gene with molecular biology data obtained on the same samples.
Chilosi
M
M
Istituto di Anatomia Patologica, Università di Verona, Italy.
Montagna
L
L
Lestani
M
M
Menestrina
F
F
Mariuzzi
G M
GM
D014408:Biomarkers, Tumor / Q000032:analysis*; D016147:Genes, Tumor Suppressor; D006689:Hodgkin Disease / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D017730:Ki-1 Antigen / Q000032:analysis*; D016539:Reed-Sternberg Cells / Q000737:chemistry* / Q000276:immunology
D014408:Biomarkers, Tumor; D017730:Ki-1 Antigen