Reversal of terminal differentiation mediated by p107 in Rb-/- muscle cells.
The terminal differentiation of mammalian muscle cells requires the tumor suppressor retinoblastoma protein (Rb). Unlike their wild-type counterparts, multinucleated myotubes from mouse cells deficient in Rb (Rb-/-) were induced by serum to re-enter the cell cycle. Development of the myogenic phenotype in Rb-/- cells correlated with increased expression of p107, which interacted with myogenic transcription factors. Serum-induced cell cycle reentry, on the other hand, correlated with decreased p107 expression. Thus, although p107 could substitute for Rb as a cofactor for differentiation, it could not maintain the terminally differentiated state in Rb-/- myotubes.
Schneider
J W
JW
Department of Cardiology, Children's Hospital, Harvard Medical School, Boston, MA 02115.
Gu
W
W
Zhu
L
L
Mahdavi
V
V
Nadal-Ginard
B
B
D000818:Animals; D001769:Blood; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D015870:Gene Expression; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D051379:Mice; D009132:Muscles / Q000166:cytology* / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000235:genetics / Q000502:physiology*; D050720:Retinoblastoma-Like Protein p107; D015533:Transcriptional Activation; D014162:Transfection; D014407:Tumor Cells, Cultured
D003470:Culture Media; C503799:MYOG protein, human; C503801:Myog protein, mouse; D018008:Myogenin; D009687:Nuclear Proteins; D011506:Proteins; C493844:RBL1 protein, human; C489485:Rbl1 protein, mouse; D011993:Recombinant Fusion Proteins; D016160:Retinoblastoma Protein; D050720:Retinoblastoma-Like Protein p107