Comparison of virus neutralisation and enzyme-linked immunosorbent assay for the identification of antibodies against vesicular stomatitis (Indiana 3) virus.
Bovine, equine and swine sera from areas free from vesicular stomatitis virus (VSV) Indiana 3 (IND3)--namely Argentina, Chile, Italy and Uruguay--and endemic areas (in Brazil) were examined for anti-VSV IND3 virus antibodies in order to compare results obtained using the virus neutralisation (VN) test and liquid-phase blocking enzyme-linked immunosorbent assay (ELISA). Statistical analysis of the data showed close agreement between the two techniques (K = 0.92). The ELISA gave a higher specificity, positive predictive value and efficiency than the VN test. The ELISA offers the additional advantage of using a non-infectious antigen and can therefore be used for antibody quantification in diagnostic laboratories in VSV-free areas to support epidemiological surveillance programmes.
Allende
R
R
Pan American Foot-and-Mouth Disease Center (PANAFTOSA/HPV/PAHO), Rio de Janeiro, Brazil.
Germano
P M
PM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis; D006736:Horses; D009500:Neutralization Tests / Q000662:veterinary*; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary; D012680:Sensitivity and Specificity; D013280:Stomatitis / Q000175:diagnosis / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*
D000914:Antibodies, Viral