Sequences of the cobalamin biosynthetic genes cobK, cobL and cobM from Rhodococcus sp. NI86/21.
Sequence analysis of a 5753-bp genomic fragment of Rhodococcus sp. NI86/21 revealed the presence of three homologues of known cobalamin biosynthetic genes. One of these genes encodes a protein showing strong homology with the precorrin-6x reductase (CobK) of Pseudomonas denitrificans. In addition, the rhodococcal homologue of the corrin methyltransferases, CobM from P. denitrificans and CbiF from Salmonella typhimurium, was identified. The protein deduced from a third Rhodococcus gene aligns well with CobL of P. denitrificans and with the protein pair CbiE/CbiT of S. typhimurium, which are involved in corrin methylation and decarboxylation.
De Mot
R
R
F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
Nagy
I
I
Schoofs
G
G
Vanderleyden
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics; D011549:Pseudomonas / Q000201:enzymology; D012240:Rhodococcus / Q000235:genetics*; D012486:Salmonella typhimurium / Q000201:enzymology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014805:Vitamin B 12 / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D001426:Bacterial Proteins; D010088:Oxidoreductases; C072472:precorrin 6x oxidoreductase; D008780:Methyltransferases; D014805:Vitamin B 12