The effect of heparin and related proteoglycans on allergen and PAF-induced eosinophil infiltration.
Exposure of normal guinea pigs to an aerosol of PAF induced a selective increase in the percentage of eosinophils in bronchoalveolar lavage (BAL) fluid 24 h after challenge. Challenge of actively sensitised guinea pigs with an aerosol of ovalbumin also induced a selective increase in the percentage of eosinophils recovered in BAL fluid 24 h post challenge. Pretreatment of actively sensitised guinea pigs or normal guinea pigs with unfractionated heparin significantly reduced such eosinophil infiltration induced by allergen or PAF challenge respectively, although higher amounts of heparin were required to inhibit antigen induced eosinophil infiltration. Similar effects were also observed following treatment with the low molecular weight heparin-like material ORG 10172 but not the anionic molecule polyglutamic acid or high molecular weight dextrans. These results suggest that proteoglycans may possess anti-allergic activity that is not necessarily related to either such molecules being anionic in nature nor to anti-coagulant activity.
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Department of Pharmacology, King's College, University of London, UK.
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D000336:Aerosols; D000485:Allergens / Q000494:pharmacology*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D004804:Eosinophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D006025:Glycosaminoglycans / Q000494:pharmacology; D006168:Guinea Pigs; D006493:Heparin / Q000494:pharmacology*; D006497:Heparan Sulfate; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D010972:Platelet Activating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D011509:Proteoglycans / Q000494:pharmacology*
D000336:Aerosols; D000485:Allergens; D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006493:Heparin; D006497:Heparan Sulfate; D010047:Ovalbumin; D010972:Platelet Activating Factor; D011509:Proteoglycans; C035838:danaparoid