Carboxyatractylate inhibits the potentiating effect of lipophylic cation TPP+ on uncoupling activity of fatty acid.
The effect of TPP+ on the fatty acid or FCCP-induced uncoupling in rat heart mitochondria was studied. It was found that (a) TPP+ increases the stimulation of oxygen consumption by palmitic acid or FCCP in the presence of oligomycin, (b) TPP+ greatly enhances the palmitic acid or FCCP-induced delta psi decrease. Both effects of TPP+ were strongly suppressed by carboxyatractylate in the case of palmitate but were not in the case of FCCP. The role of ATP/ADP-antiporter in the TPP+ and palmitic acid effects is discussed.
Dedukhova
V I
VI
A.N. Belozersky Institute of Physical-Chemical Biology, Moscow State University, Russia.
Mokhova
E N
EN
Starkov
A A
AA
Leikin YuN
D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D009861:Onium Compounds / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology*; D010619:Phenazines / Q000378:metabolism; D051381:Rats; D013050:Spectrometry, Fluorescence; D014475:Uncoupling Agents / Q000494:pharmacology
D005456:Fluorescent Dyes; D009840:Oligomycins; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D010169:Palmitic Acids; D010619:Phenazines; D014475:Uncoupling Agents; D001278:Atractyloside; D019308:Palmitic Acid; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; C003853:carboxyatractyloside; C013289:tetraphenylphosphonium; C009195:safranine T