title: Induction of cell surface peptidase activity: a global response to cell stress correlated with apoptosis.
abstract: We have previously characterized the stimulation of HeLa cell surface peptidase activity directed toward a nonapeptide substrate in response to low fluences of ultraviolet irradiation [Brown et al. (1993): J Cell Biochem 51:102-115]. To explore the hypothesis that this comprised a global response to cell stress featuring the interruption of DNA synthesis, a variety of agents affecting macromolecular synthesis were applied to HeLa cell cultures. Actinomycin D, 5,6-dichloro-1 beta-ribofuranosyl benzimadazole, mitomycin C, ultraviolet light, and cycloheximide at doses which inhibited cell growth, but fell short of increasing the proportion of cells which had lost cell membrane impermeability to trypan blue, resulted in the concentration dependent increase in both amino- and endo-peptidase activities of intact HeLa cell cultures. gamma-Irradiation, despite inhibiting an increase in cell number over a 20-h observation period, had no effect on the expressed level of cell surface peptidase activity nor did the accumulation of cells in S or G2 phase by thymidine parasynchronization. Some of these agents were found to increase the proportion of cells in the culture undergoing apoptosis (programmed cell death), and a strong correlation was found between the extent of apoptosis and the degree of elevation in cell surface peptidase activity. Higher concentrations of perturbants in some instances increased the percentage of cells that were nonviable and an associated release of intracellular proteases overwhelmed the linear correlation with apoptotic cells. The present data do not distinguish between a homogeneous elevation of surface peptidase activity in all cells of treated cultures or the heterogeneous increase in only preapoptotic or apoptotic cells. Since sunburn of the skin increases both the occurrence of apoptotic keratinocytes (sunburn cells) in the affected epidermis and the release of membrane bound cell activators such as transforming growth factor alpha, it is suggested by way of extrapolation of these in vitro results, that the increase in cell surface proteolytic activity plays an integral part in the reparative responses of the epidermal cells in vivo.
authors.affiliation: Queensland Cancer Fund Research Unit, Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
authors.forename: S B
authors.initials: SB
authors.lastname: Brown
authors.forename: R M
authors.initials: RM
authors.lastname: Kluck
authors.forename: K A
authors.initials: KA
authors.lastname: Ellem
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology; D010450:Endopeptidases / Q000096:biosynthesis*; D004790:Enzyme Induction; D006367:HeLa Cells / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis*; D016685:Mitomycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013312:Stress, Physiological / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D016211:Transforming Growth Factor alpha / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D008565:Membrane Proteins; D010446:Peptide Fragments; D011500:Protein Synthesis Inhibitors; D016211:Transforming Growth Factor alpha; D003609:Dactinomycin; D016685:Mitomycin; D004004:Dichlororibofuranosylbenzimidazole; D003513:Cycloheximide; D010450:Endopeptidases; D013755:Tetradecanoylphorbol Acetate