title: Human type II hyperlipoproteinemia enhances platelet-collagen adhesion in flowing nonanticoagulated blood.
abstract: We investigated the effects of high plasma lipid levels on platelet adhesion and platelet thrombus formation in nonanticoagulated human blood on collagen fibrils at an arterial wall shear rate of 2600 seconds-1. Nonanticoagulated blood was drawn directly at a flow rate of 10 mL/min for 3 minutes from an antecubital vein of patients with type IIa (n = 5) and type IIb (n = 4) hyperlipoproteinemia over purified human type III collagen fibrils that were positioned on a plastic coverslip in a parallel-plate perfusion chamber. Results were compared with those obtained in healthy individuals with normal lipid plasma levels (n = 9). Blood-collagen interactions were quantified by morphometry as platelet-collagen adhesion, thrombus volume, and fibrin deposition. Platelet-collagen adhesion in the two groups of patients was significantly higher than in healthy individuals (70.7 [61.2 to 82.0] and 70.3 [66.4 to 81.0] in types IIa and IIb patients, respectively, versus 51.2 [44.5 to 68.6] in control subjects; P < .05. All values are percent median [range]). In contrast, the thrombus volume was similar in the three groups (11.3 [8.0 to 13.0], 9.6 [6.4 to 15.3], and 10.2 [6.8 to 16.1] microns3/microns2 [range], respectively). Differences in fibrin deposition were not observed. Thus, it appears that platelet-collagen adhesion is augmented in patients with type IIa and IIb hyperlipoproteinemia, indicating that the process of thrombogenesis is hastened in these patients.
authors.affiliation: Laboratoire d'Hémostase, Hôpital Purpan, Toulouse, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Cadroy
authors.forename: S
authors.initials: S
authors.lastname: Lemozy
authors.forename: A
authors.initials: A
authors.lastname: Diquélou
authors.forename: J
authors.initials: J
authors.lastname: Ferrières
authors.forename: P
authors.initials: P
authors.lastname: Douste-Blazy
authors.forename: B
authors.initials: B
authors.lastname: Boneu
authors.forename: K S
authors.initials: KS
authors.lastname: Sakariassen
mesh_terms: D000328:Adult; D000368:Aged; D001777:Blood Coagulation; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D003094:Collagen / Q000097:blood*; D005260:Female; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010973:Platelet Adhesiveness / Q000502:physiology*; D014280:Triglycerides / Q000097:blood
chemical_list: D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D014280:Triglycerides; D005337:Fibrin; D003094:Collagen; D002784:Cholesterol