11-Hydroxythromboxane B2 dehydrogenase is identical to cytosolic aldehyde dehydrogenase.
11-Hydroxythromboxane B2 dehydrogenase purified from porcine kidney has been identified as cytosolic aldehyde dehydrogenase (EC 1.2.1.3). This identification is based on protein characteristics, sequence analysis of one proteolytic digest, blocked N-terminus, subunit molecular mass of 55 kDa, and enzymatic activities. The sequence difference with the human enzyme is 7.5% in the fragments analyzed (29 exchanges of 388 positions, corresponding to the expected species variability for cytosolic aldehyde dehydrogenase). The substrate thromboxane B2 contains a hemiacetal in its ring structure, but the reaction most likely proceeds via the aldehyde form of the substrate. This finding is in agreement with the proposed metabolism of 4-hydroxycyclophosphamide and highlights the possibility that molecules containing a hemiacetal structure can function as substrates for aldehyde dehydrogenase.
Westlund
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Department of Woman and Child Health, Karolinska Hospital, Stockholm, Sweden.
Fylling
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AC
Cederlund
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Jörnvall
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H
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping; D017386:Sequence Homology, Amino Acid; D013552:Swine
D000596:Amino Acids; D046911:Macromolecular Substances; D010446:Peptide Fragments; D000429:Alcohol Oxidoreductases; C049239:thromboxane dehydrogenase; D000444:Aldehyde Dehydrogenase