title: 4-Dimethylaminophenethylamine, a sensitive, specific, electrochemically detectable monoamine oxidase-B substrate.
abstract: 4-Dimethylaminophenethylamine (DMAPEA) was characterized as an MAO substrate. This compound was unaffected by MAO-A, while its oxidation by MAO-B was linear as a function of both time and enzyme concentration, with Km = 5.8 microM and Vmax = 21.2 pmol/min/mg protein, using a crude rat brain mitochondrial suspension as source of MAO. Both DMAPEA and its oxidation product, 4-dimethylaminophenylacetic acid (DMAPAA), can be detected electrochemically at 0.85 V. The high MAO-B affinity and selectivity of DMAPEA, together with its low oxidation potential, make this molecule a unique tool to determine MAO-B activity in a wide variety of tissue preparations using HPLC-ED.
authors.affiliation: Cell Biology Division, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
authors.forename: M
authors.initials: M
authors.lastname: Reyes-Parada
authors.forename: M C
authors.initials: MC
authors.lastname: Scorza
authors.forename: R
authors.initials: R
authors.lastname: Silveira
authors.forename: F
authors.initials: F
authors.lastname: Dajas
authors.forename: B K
authors.initials: BK
authors.lastname: Cassels
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D003010:Clorgyline / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010627:Phenethylamines / Q000737:chemistry / Q000378:metabolism*; D010648:Phenylacetates / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D012642:Selegiline / Q000494:pharmacology
chemical_list: D010627:Phenethylamines; D010648:Phenylacetates; C030748:4-N,N-dimethylaminophenylacetic acid; D012642:Selegiline; C087509:4-dimethylaminophenethylamine; D008995:Monoamine Oxidase; D003010:Clorgyline