Estrogen-regulated expression of a growth arrest specific gene (gas-1) in rat uterus.
gas-1 belongs to a family of growth arrest specific genes negatively regulated after growth induction of arrested cells. We report the expression of gas-1 in an in vivo system of cell proliferation. gas-1 mRNA accumulates progressively in the uterus of ovariectomized rats with a peak at three weeks after surgery. After estrogen treatment gas-1 mRNA levels decrease within two hours, at a time when c-myc expression is greatly increased, and return to pretreatment levels at 48 hours. Treatment with cycloheximide does not prevent estrogen-induced down-regulation of gas-1 mRNA levels. The present results show that: i) estrogen affects the uterine growth state by regulating the expression of both positively and negatively acting genes, ii) gas-1 expression is controlled by cellular growth state also in vivo.
Ferrero
M
M
Istituto di Patologia Generale e Centro di Studio sulla Patologia Cellulare, CNR, Milano, Italy.
Cairo
G
G
D000818:Animals; D015152:Blotting, Northern; D003513:Cycloheximide / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005293:Ferritins / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes; D016259:Genes, myc; D010052:Ovariectomy; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D004958:Estradiol; D005293:Ferritins; D003513:Cycloheximide