Pulmonary infections of gut origin after major liver resection in rats.
OBJECTIVE
To assess the incidence of translocation of enteric bacteria to the lungs in the early postoperative period (1, 2, 4, and 6 hours) after sham operation, 70% hepatectomy, and 90% hepatectomy.


METHODS
Randomised laboratory experiment.


METHODS
University department.


METHODS
96 Adult male Sprague-Dawley rats.


METHODS
Sham operation (n = 24), 70% hepatectomy (n = 36), and 90% hepatectomy (n = 36). Injection of 1 ml (1.5 x 10(7) cpm/rat) 125I-labelled heat-killed Escherichia coli 15 minutes before the animals were killed.


METHODS
Arterial blood gas analyses, wet: dry weight ratio of lungs, and measurements of pulmonary isotopic flux (amount of 125I radioactivity in the lungs compared with that in the blood) 1, 2, 4, and 6 hours after operation. Quantitative microbiology of lungs.


RESULTS
There was no pulmonary oedema in those rats that underwent sham operation. Pulmonary oedema had developed by 6 hours after 70% hepatectomy and by 2 hours after 90% hepatectomy. The lung:blood ratio of 125I-labelled bacteria increased as the pulmonary oedema developed, but pulmonary oxygen exchange was not influenced during the first 6 hours after hepatectomy. The incidence of bacterial translocation increased with the extent of hepatectomy and with the length of time after operation.


CONCLUSIONS
Bacterial translocation from the gut could lead to pulmonary infection after major liver resection in rats.
Wang
X
X
Department of Surgery, Lund University, Sweden.
Wang
Q
Q
Soltesz
V
V
Andersson
R
R
D000818:Animals; D002465:Cell Movement; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000209:etiology* / Q000382:microbiology; D006498:Hepatectomy / Q000009:adverse effects*; D007422:Intestines / Q000382:microbiology*; D008168:Lung / Q000382:microbiology*; D008171:Lung Diseases / Q000209:etiology* / Q000382:microbiology; D008297:Male; D011184:Postoperative Period; D011654:Pulmonary Edema / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley