Expression of human lactoferrin in milk of transgenic mice.
The expression of human lactoferrin (hLF) in the milk of transgenic mice is described. Regulatory sequences derived from the bovine alpha S1-casein gene were fused to the coding sequence of the hLF cDNA and several lines of transgenic mice were generated. Human LF RNA was detected exclusively in the mammary gland of lactating females and only after the onset of lactation. No aberrant RNA products could be detected using northern blotting and primer extension analysis. The hLF concentrations in the milk ranged from less than 0.1 to 36 micrograms ml-1. Human LF thus expressed did not differ from human milk derived LF, with respect to molecular mass and immunoreactivity with monoclonal and polyclonal antibodies.
Platenburg
G J
GJ
GenePharming Europe B.V. Niels Bohrweg, Leiden, The Netherlands.
Kootwijk
E P
EP
Kooiman
P M
PM
Woloshuk
S L
SL
Nuijens
J H
JH
Krimpenfort
P J
PJ
Pieper
F R
FR
de Boer
H A
HA
Strijker
R
R
D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics; D002417:Cattle; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007774:Lactation / Q000378:metabolism; D007781:Lactoferrin / Q000096:biosynthesis* / Q000235:genetics*; D008297:Male; D008321:Mammary Glands, Animal / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics
D002364:Caseins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D007781:Lactoferrin