Protein-free culture of Vero cells: a substrate for replication of human pathogenic viruses.
A protein-free chemically defined medium designated PFEK-1 was developed for culture of VERO cells on polyvinyl formal (PVF) culture surface without serum or other macromolecular supplements. VERO cells proliferated in PFEK-1 medium on PVF surface to a similar extent as cells in serum-supplemented medium without previous adaptation from serum-containing conditions. The protein-free culture infected with coxsackievirus B4, herpes simplex virus types 1 and 2, measles virus and poliovirus types 1, 2 and 3 developed viral titers comparable to those found in conventionally grown cells. The results demonstrated that VERO cells in protein-free culture provide a sensitive substrate for the production of human pathogenic viruses which are not contaminated by serum or other protein factors usually added to a culture medium.
Cinatl
J
J
Centre of Hygiene, J. W. Goethe-University, Frankfurt a. M., Fed. Rep. of Germany.
Cinatl
J
J
Rabenau
H
H
Rapp
J
J
Kornhuber
B
B
Doerr
H W
HW
D000818:Animals; D016895:Culture Media, Serum-Free / Q000032:analysis / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000254:growth & development / Q000502:physiology; D006801:Humans; D008459:Measles virus / Q000254:growth & development / Q000502:physiology; D017955:Poliovirus / Q000254:growth & development / Q000502:physiology; D018139:Simplexvirus / Q000254:growth & development / Q000502:physiology; D014709:Vero Cells / Q000166:cytology / Q000382:microbiology*; D014776:Virus Cultivation / Q000379:methods*; D018406:Virus Physiological Phenomena; D014779:Virus Replication; D014780:Viruses / Q000254:growth & development*
D016895:Culture Media, Serum-Free