Expression of insulin-like growth factor II by a gastric carcinoma associated with hypoglycaemia.
A gastric cancer with liver metastases was associated with low morning levels of plasma glucose (24 mg/dl), insulin (< 2.5 microU/ml) and growth hormone (0.23 ng/ml). Primary and metastatic tumour tissue stained positively with anti-insulin-like growth factor II (IGF-II) monoclonal antibody. Western immunoblot analysis revealed a high molecular weight IGF-II in the serum: 15 kDa (normal: 7.5 kDa). Postmortem reverse transcription polymerase chain reaction on mRNA from both sites revealed 471 base pairs size cDNA encoding prepro-IGF-II. These results suggest that the gastric carcinoma encoded, expressed, and secreted IGF-II, probably causing the extrapancreatic tumour hypoglycaemia.
Horiuchi
T
T
Third Department of Medicine, Teikyo University School of Medicine, Ichihara Hospital, Chiba, Japan.
Shinohara
Y
Y
Sakamoto
Y
Y
Hizuka
N
N
Watabe
T
T
Mokuda
O
O
Tanaka
K
K
Shimizu
N
N
Sugano
I
I
Nagao
K
K
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D015153:Blotting, Western; D006801:Humans; D007003:Hypoglycemia / Q000150:complications*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D008113:Liver Neoplasms / Q000556:secondary; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000150:complications / Q000378:metabolism* / Q000473:pathology
D007335:Insulin-Like Growth Factor II