P-cadherin and E-cadherin are co-expressed in MDCK cells.
The Madin-Darby canine kidney (MDCK) cell line is a valuable model of epithelial cell function and the role of E (epithelial)-cadherin in MDCK cell activity is well-appreciated. Northern and immunoblot analyses were used here to demonstrate that, in addition to E-cadherin, MDCK cell cultures also express P(placental)-cadherin. Immunocytochemistry was then used to compare the localization and detergent extractability of P- and E-cadherin. While both cadherins were found at areas of cell-cell contact, the relative ease with which P-cadherin could be extracted suggests a weaker anchorage to the actin cytoskeleton than has been demonstrated for E-cadherin, and may correspond to less permanent cell-cell interactions than those attributed to E-cadherin. The co-expression of different cadherins by MDCK cells has not been fully explored and may influence interpretations of MDCK cell function that previously considered only the activity of E-cadherin.
Wu
J C
JC
Department of Cell Biology, Neurobiology & Anatomy, Ohio State University College of Medicine, Columbus 43210.
Gregory
C W
CW
DePhilip
R M
RM
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D002450:Cell Communication; D002451:Cell Compartmentation / Q000187:drug effects; D002460:Cell Line; D004285:Dogs; D014644:Genetic Variation; D007150:Immunohistochemistry; D017830:Octoxynol / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis
D015820:Cadherins; D012333:RNA, Messenger; D017830:Octoxynol