title: Determination of free myo-inositol in milk and infant formula by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method is described for the determination of supplemental myo-inositol in infant formulas based on cows' milk. The technique incorporates pre-column derivatization with phenylisocyanate followed by reversed-phase gradient chromatography and ultraviolet detection. The protocol was also applied to a survey of free inositol at endogenous levels in the milk of cows, goats and humans. Nutritional preparations containing inositol at pharmacological levels are also amenable to a simplified isocratic analysis.
authors.affiliation: Anchor Products, Waitoa, New Zealand.
authors.forename: H E
authors.initials: HE
authors.lastname: Indyk
authors.forename: D C
authors.initials: DC
authors.lastname: Woollard
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006041:Goats; D006801:Humans; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D007294:Inositol / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry
chemical_list: D007294:Inositol