Production of the metastatic phenotype by DNA transfection in a rat mammary model.
A syngeneic, immunocompetent rat mammary model has been employed to investigate the molecular basis of the metastatic phenotype. Transfection of the benign rat mammary epithelial cell line Rama 37 with the gene for p9Ka; a small, rat, calcium-binding protein or DNA from metastatic human cell lines derived from a primary breast carcinoma or a pleural effusion yields transfectants with metastatic capabilities. Transfection of DNA from a benign human mammary cell line, a plasmid containing a cDNA for the human basic fibroblast growth factor (bFGF) gene, or the oncogene EJ-ras-1, fails to yield any transfectants expressing the metastatic phenotype.
Davies
B R
BR
Biochemistry Department, University of Liverpool.
Barraclough
R
R
Davies
M P
MP
Rudland
P S
PS
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000502:physiology; D006801:Humans; D008321:Mammary Glands, Animal / Q000166:cytology; D008325:Mammary Neoplasms, Experimental / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics / Q000502:physiology; D010641:Phenotype; D010957:Plasmids; D051381:Rats; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; D014162:Transfection
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D015513:Oncogene Proteins; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C499412:S100a4 protein, rat; C077183:human oncogene protein EJ-ras; D016222:Fibroblast Growth Factor 2; C077591:S100A4 protein, human