Comparison of methods used for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines.
Two methods for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines were compared: the direct culture test and the DNA staining method employing bisBenzimide (Hoechst No. 33258). Contamination by different species of mycoplasma was found in 39% samples tested. It is recommended to use both techniques for a reliable detection of mycoplasma contamination.
Benisheva
T
T
National Drug Institute, Sofia.
Sovová
V
V
Ivanov
I
I
Opalchenova
G
G
D001769:Blood / Q000382:microbiology*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis*; D004340:Drug Contamination; D009174:Mycoplasma / Q000302:isolation & purification*; D015203:Reproducibility of Results; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
D004269:DNA, Bacterial; D014613:Vaccines, Attenuated; D014765:Viral Vaccines