title: Limited heterogeneity in the T-cell receptor V-gene usage in lymphocytes infiltrating human colorectal tumours.
abstract: The presence of T lymphocytes in solid tumours may reflect an ongoing immune response against the transformed cells. We have used polymerase chain reaction (PCR) technology to investigate the T-cell receptor variable-region gene (V-gene) usage in freshly isolated tumour-infiltrating lymphocytes (TILs) to look for a possible oligoclonality of T cells in the tumour area. We used 19 different V beta-family-specific primers. Peripheral blood lymphocytes and lamina propria lymphocytes from the same patients were also tested by PCR. Our results demonstrate a limited heterogeneity in the V-gene usage of TILs from seven patients with colorectal cancers, suggesting a local antigen-driven immune response at the tumour site.
authors.affiliation: Institute of Immunology and Rheumatology, National Hospital, Oslo, Norway.
authors.forename: B
authors.initials: B
authors.lastname: Ostenstad
authors.forename: M
authors.initials: M
authors.lastname: Sioud
authors.forename: T
authors.initials: T
authors.lastname: Lea
authors.forename: E
authors.initials: E
authors.lastname: Schlichting
authors.forename: M
authors.initials: M
authors.lastname: Harboe
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics* / Q000276:immunology* / Q000601:surgery; D015703:Antigens, CD / Q000032:analysis; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology; D015179:Colorectal Neoplasms / Q000097:blood / Q000235:genetics* / Q000276:immunology* / Q000601:surgery; D004247:DNA / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000235:genetics*; D012004:Rectal Neoplasms / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA