Cloning and sequencing of the chicken anaemia virus (CAV) ORF-3 gene, and the development of an ELISA for the detection of serum antibody to CAV.
Chicken anaemia virus (CAV) is a small, unclassified virus involved in anaemia and suspected of causing immunosuppression in young chickens. We have developed an ELISA for the detection of serum antibody to CAV based on cloned antigen. The gene for ORF-3 (the putative capsid protein) was cloned, sequenced and expressed in a bacterial expression system, pGEX. An ORF-3 fusion protein was used to produce an indirect ELISA.
Pallister
J
J
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
Fahey
K J
KJ
Sheppard
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D017727:Chicken anemia virus / Q000235:genetics* / Q000276:immunology; D002645:Chickens; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D016366:Open Reading Frames / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral; D004279:DNA, Viral