title: Weak N activity of En(a-) human erythrocyte membranes.
abstract: The propositus's erythrocytes with phenotype En(a-), which was found for the first time in a Japanese family, reacted more weakly with anti-N serum than the ordinary phenotype N erythrocytes. The En(a-) erythrocytes lack the major membrane sialoglycoprotein (glycophorin A) as demonstrated by Bio-Gel 1.5m gel filtration from active sialoglycoproteins, which were isolated from En(a-) erythrocyte membranes by the method of lithium diiodosalicylate (LIS)-phenol extraction. It is suggested from observation via enzyme-linked immunosorbent assay (ELISA) that N activity is derived from the glycophorin B molecule on En(a-) erythrocyte membranes.
authors.affiliation: Department of Legal Medicine, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shinozuka
authors.forename: Y
authors.initials: Y
authors.lastname: Miyata
authors.forename: K
authors.initials: K
authors.lastname: Ohyama
authors.forename: S
authors.initials: S
authors.lastname: Takei
authors.forename: T
authors.initials: T
authors.lastname: Yamakawa
authors.forename: K
authors.initials: K
authors.lastname: Kurihara
authors.forename: J
authors.initials: J
authors.lastname: Yanagida
mesh_terms: D000941:Antigens / Q000097:blood*; D002850:Chromatography, Gel; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000276:immunology*; D006021:Glycophorins / Q000032:analysis / Q000172:deficiency; D006801:Humans; D007564:Japan; D008951:MNSs Blood-Group System / Q000276:immunology*; D010641:Phenotype
chemical_list: D000941:Antigens; D006021:Glycophorins; D008951:MNSs Blood-Group System