title: Cytogenetic abnormalities in two cases of neuroblastoma.
abstract: Neuroblastomas are common solid tumors in children. We report chromosome analysis of two neuroblastomas, each studied at diagnosis and at recurrence. The first case was a clinical stage D tumor which showed 45,X-Y, add(1)(p34),der(15)t(Y;15)(q11;p13), and double minutes on cytogenetic analysis at diagnosis. At recurrence, the same structural abnormalities were present along with a homogeneously staining region (hsr) at 8q22, 19p12, or 3p23 in each of three related clones. The hsr were shown to represent amplification of the N-myc gene by in situ hybridization. Cytogenetic analysis of the second tumor, stage D-S, showed 48-54,XX,der(1)add (1)(q41), +2, +7, +7, inv(9), +17, + mar. The lack of demonstrative involvement of 1p or visible evidence of gene amplification has also characterized the limited number of D-S specimens previously described, suggesting that stage D-S neuroblastoma indeed differs from stage D disease at the genetic level.
authors.affiliation: Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors.forename: R
authors.initials: R
authors.lastname: Lo
authors.forename: E
authors.initials: E
authors.lastname: Perlman
authors.forename: A L
authors.initials: AL
authors.lastname: Hawkins
authors.forename: R
authors.initials: R
authors.lastname: Hayashi
authors.forename: D S
authors.initials: DS
authors.lastname: Wechsler
authors.forename: A T
authors.initials: AT
authors.lastname: Look
authors.forename: C A
authors.initials: CA
authors.lastname: Griffin
mesh_terms: D000008:Abdominal Neoplasms / Q000235:genetics*; D002869:Chromosome Aberrations; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D007621:Karyotyping; D008297:Male; D009447:Neuroblastoma / Q000235:genetics*; D012186:Retroperitoneal Neoplasms / Q000235:genetics