Detection of Epstein-Barr virus in a case of undifferentiated nasopharyngeal carcinoma by in situ hybridization with digoxigenin-labelled PCR-generated probes.
A case of nasopharyngeal carcinoma is presented. Epstein-Barr viral genome was identified in the neoplastic cells by in situ hybridization with digoxigenin-labelled polymerase chain reaction-generated probes. We report the development of this technique in paraffin-embedded sections and propose that such identification may prove valuable for the diagnosis of this tumour in routine material.
Delvenne
P
P
Department of Pathology, University Hospital of Liège, Belgium.
Kaschten
B
B
Deneufbourg
J M
JM
Demanez
L
L
Stevenaert
A
A
Reznik
M
M
Boniver
J
J
D000328:Adult; D001483:Base Sequence; D002277:Carcinoma / Q000382:microbiology* / Q000473:pathology; D004076:Digoxigenin; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D008279:Magnetic Resonance Imaging; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction
D015335:Molecular Probes; D004076:Digoxigenin