Cytochemical features common to nucleoli and cytoplasmic nucleoloids of Olea europaea meiocytes: detection of rRNA by in situ hybridization.
We used light and electron microscopic techniques to study the composition of cytoplasmic nucleoloids during meiotic division in Olea europaea. Nucleoloids were found in two clearly distinguishable morphological varieties: one similar in morphology to the nucleolus, and composed mainly of dense fibrillar component, and another surrounded by many ribosome-like particles. Cytochemical and immunocytochemical techniques showed similar reactivities in nucleoloids and the nucleolus: both are ribonucleoproteic in nature, and possess argyrophillic, argentaffinic and highly phosphorylated proteins. Immunohistochemical techniques failed to detect DNA in either structure. In situ hybridization to a 18 S rRNA probe demonstrated the presence of ribosomal transcripts in both the nucleolus and nucleoloids. These similarities in morphology and composition may reflect similar functionalities.
Alché
J D
JD
Plant Biochemistry, Molecular and Cellular Biology Department, Estación Experimental del Zaidín, CSIC, Granada, Spain.
Fernández
M C
MC
Rodríguez-García
M I
MI
D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D002479:Inclusion Bodies / Q000378:metabolism / Q000648:ultrastructure; D008540:Meiosis; D008854:Microscopy, Electron; D010940:Plant Proteins / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism*; D014197:Trees / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*
D010940:Plant Proteins; D012337:RNA, Ribosomal, 18S