Studies in red blood cell preservation. 7. In vivo and in vitro studies with a modified phosphate-ammonium additive solution.
Studies were conducted to examine whether an experimental additive solution (EAS-2) containing, in mM: 20 NH4Cl, 30 Na2HPO4, 2 adenine, 110 dextrose, 55 mannitol, pH 7.15, would be useful to extend the storage shelf life of human RBCs. With 6 pairs of split units, ATP concentrations were better maintained for 12 weeks with EAS-2 than with Adsol (1.8 vs. 1.1 mumol/g Hb, respectively, p = 0.002). Autologous 24-hours 51Cr viability values for split units in the same donors were: on 6 paired units at 8 weeks, EAS-2 87.0 +/- 4.5%, Adsol 72.6 +/- 2.3%, p = 0.004; on 11 paired units at 9 weeks, EAS-2 79.5 +/- 7.1%, Adsol 68.2 +/- 10.1%, p = 0.0003. The data suggest that packed RBCs stored for 9 weeks with EAS-2 will be suitable for transfusion following the removal of supernatant with a single washing step.
Greenwalt
T J
TJ
Hoxworth Blood Center, University of Cincinnati Medical Center, Ohio.
Dumaswala
U J
UJ
Dhingra
N
N
Allen
C M
CM
Silberstein
E B
EB
D000225:Adenine; D000643:Ammonium Chloride; D001793:Blood Preservation; D002470:Cell Survival / Q000502:physiology; D002860:Chromium Radioisotopes; D004912:Erythrocytes; D005947:Glucose; D006801:Humans; D008353:Mannitol; D010710:Phosphates; D012965:Sodium Chloride; D012996:Solutions
C037839:ADSOL; D002860:Chromium Radioisotopes; D010710:Phosphates; D012996:Solutions; D000643:Ammonium Chloride; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine; C018279:sodium phosphate