title: Variable selectivity of the Hitachi chemistry analyzer chloride ion-selective electrode toward interfering ions.
abstract: Chloride measurements by ion-selective electrodes are vulnerable to interference by anions such as iodide, thiocyanate, nitrate, and bromide. We have found that the degree of interference of these anions on the Hitachi chemistry analyzer chloride electrode varies from electrode to electrode and this variation can even occur within the same lot of membrane. This variation is not dependent upon the length of time the cartridge has been in the analyzer because no correlation existed between the usage time and the electrode response to interfering ions. Neither is this variation due to the deterioration of the electrode because all electrodes tested had calibration slopes within the manufacturer's specification. Our study, however, showed that even after repeated exposure to a plasma sample containing 2 mM thiocyanate, the chloride electrode was still able to accurately measure the chloride in plasma without thiocyanate, thus confirming that a carryover effect does not exist from a previous thiocyanate-containing sample.
authors.affiliation: Department of Clinical Biochemistry, Toronto General Division, Toronto Hospital, Ontario, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: E P
authors.initials: EP
authors.lastname: Diamandis
authors.forename: A
authors.initials: A
authors.lastname: Lane
authors.forename: A D
authors.initials: AD
authors.lastname: Baines
mesh_terms: D000838:Anions; D016477:Artifacts; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D002712:Chlorides / Q000097:blood*; D005260:Female; D006801:Humans; D017736:Ion-Selective Electrodes; D008875:Middle Aged
chemical_list: D000838:Anions; D002712:Chlorides