Binding properties of protein A and protein G for human IgE.
We studied the binding of protein A and protein G to human monoclonal and polyclonal IgE. An ELISA was used to measure human IgE. Protein A-Sepharose did not bind to monoclonal IgE, but bound to 12-14% of serum polyclonal IgE and to -3% of the polyclonal IgE purified from the serum. No difference in binding to protein A was found between IgE from an allergic patient and that from a non-allergic subject. Protein G bound to neither polyclonal IgE nor monoclonal IgE. Thus, protein G is better than protein A with respect to separation of IgE from IgG.
Peng
Z
Z
Department of Paediatrics, University of Manitoba, Winnipeg, Canada.
Becker
A B
AB
Simons
F E
FE
D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000378:metabolism*
C029152:G-substrate; D009419:Nerve Tissue Proteins; D013205:Staphylococcal Protein A; D007073:Immunoglobulin E