Simultaneous determination of verapamil and celiprolol in human plasma.
A simple and reproducible method for the simultaneous determination of the beta 1-selective adrenergic blocker, celiprolol, and the calcium antagonist, verapamil, in human plasma is described. It involves a two-step liquid-liquid extraction and separation using a C18 column with ultraviolet detection at 237 nm. Deacetyldiltiazem is used as the internal standard. Within-day and between-day coefficients of variation are less than 10%. The lower limits of detection are 4, 2, and 4 ng/mL for celiprolol, deacetyldiltiazem, and verapamil, respectively. The assay has clinical applicability.
Rutledge
D R
DR
University of Florida, College of Pharmacy, Gainesville 32610.
Abadi
A H
AH
Lopez
L M
LM
D017272:Celiprolol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014700:Verapamil / Q000097:blood*
D014700:Verapamil; D017272:Celiprolol