Evidence for eosinophil activation in eosinophilic cystitis.
Eosinophilic cystitis (EC) is a rare condition. Recent studies have shown that activated eosinophils release cytotoxic cationic proteins which can induce tissue damage. Moreover, in vitro studies have shown that interleukin-5 (IL-5) is a cytokine able to attract and activate eosinophils. The goal of this study was to detect a possible activation of eosinophils in EC using electron microscopy, in situ hybridization with an IL-5 RNA probe and immunochemistry with a specific anti-IL-5 antibody. Using these combined methods in a typical case of EC, we found numerous activated eosinophils synthesizing and secreting IL-5 protein. IL-5 could enhance the activation of eosinophils and their cytotoxic potential in bladder tissues. This mechanism might explain the chronicity of the lesions in EC.
Dubucquoi
S
S
Department of Pathology C, Hôpital Calmette, CHU Lille, France.
Janin
A
A
Desreumaux
P
P
Rigot
J M
JM
Copin
M C
MC
François
M
M
Torpier
G
G
Capron
M
M
Gosselin
B
B
D003556:Cystitis / Q000378:metabolism* / Q000473:pathology; D004802:Eosinophilia / Q000378:metabolism* / Q000473:pathology; D004804:Eosinophils / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D015848:Interleukin-5 / Q000235:genetics / Q000378:metabolism*; D008854:Microscopy, Electron; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism*; D001743:Urinary Bladder / Q000737:chemistry / Q000473:pathology*
D015848:Interleukin-5; D012333:RNA, Messenger