Interaction of small ribosomal and transfer RNAs with a protein from Leishmania donovani.
Using synthetic antisense RNA from the 5'-untranslated region of the beta-tubulin gene as probe in gel retardation assays, a heat stable RNA-binding factor was identified in promastigotes of the kinetoplastid protozoan Leishmania donovani. The same or similar factors interact with several small ribosomal RNA (srRNA) species and, more weakly, with tRNA, as shown by binding and competition experiments. Deletion analysis indicated involvement of repeated purine-rich motifs on the antisense RNA, in the reaction. Related, conserved motifs occur on at least two of the srRNAs. By a modified Western blot assay, the RNA-binding species was identified as a single, small polypeptide. The activity is apparently specific for the promastigote stage of the parasite, being undetectable in amastigotes. The properties of this RNA-binding factor suggest that it is a novel, previously uncharacterized protein.
Ghosh
A
A
Genetic Engineering Laboratory, Indian Institute of Chemical Biology, Calcutta.
Ghosh
T
T
Ghosh
S
S
Das
S
S
Adhya
S
S
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D015153:Blotting, Western; D016054:DNA, Protozoan; D007893:Leishmania donovani / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000378:metabolism*; D016372:RNA, Antisense / Q000378:metabolism; D016053:RNA, Protozoan / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D016372:RNA, Antisense; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; D014404:Tubulin; D012343:RNA, Transfer