Interleukin-6 production by human hepatoma lines is related to a low degree of cell differentiation.
In this study, we showed by Northern blot analysis and bioassays that scarcely differentiated human hepatoma cell line HA22T/VGH constitutively produces interleukin-6 (IL6). This cytokine was produced neither by moderately differentiated Li7A nor by well-differentiated HepG2 human hepatoma cell lines. The finding that transcripts for IL6 were not present in 2.2.15 cells, a HepG2-derived clone transfected with the intracellular replicative form of hepatitis B virus (HBV) DNA, suggests that production of this cytokine is not affected by HBV-encoded gene products, but is more likely related to the dedifferentiated state of hepatoma cells.
Paroli
M
M
Fondazione Andrea Cesalpino, I Clinica Medica, Università La Sapienza, Roma, Italy.
Carloni
G
G
Alfani
E
E
De Petrillo
G
G
Barnaba
V
V
D001681:Biological Assay; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008113:Liver Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D015850:Interleukin-6; D012333:RNA, Messenger