[Purification and immunochemical characteristics of neutral Mn-dependent DNAase of chromatin].
A scheme for purification of neutral Mn-dependent chromatin DNAase has been developed. The two-step purification procedure, which includes chromatography on hydroxyapatite and isoelectrofocusing, allows one to obtain preparative amounts of the enzyme having a specific activity of more than 3000 u./mg, molecular mass of 41 kDa and pI of 9.2.
Vinter
V G
VG
Zotkina
N L
NL
Zo
S Kh
SKh
Abramova
Z I
ZI
D002843:Chromatin / Q000378:metabolism*; D002850:Chromatography, Gel; D003851:Deoxyribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D007525:Isoelectric Focusing; D008345:Manganese / Q000378:metabolism*; D008970:Molecular Weight
D002843:Chromatin; D008345:Manganese; D003851:Deoxyribonucleases
Ochistka i immunokhimicheskaia kharakteristika neĭtral'noĭ Mn-zavisimoĭ DNKazy khromatina.