Atrial natriuretic peptide and glucagon release in experimental intestinal ischaemia and reperfusion.
Intestinal ischaemia and reperfusion cause changes in cardiovascular and pulmonary function. In a rat model, the plasma concentrations of atrial natriuretic peptide (ANP) and pancreatic glucagon rose on reperfusion after 20 min of intestinal ischaemia, coinciding with significant arterial hypotension: mean(s.e.m.) ANP 79(13) versus control 36(4) fmol ml-1 (P < 0.01); and mean(s.e.m.) glucagon 22(2) versus control 10(1) fmol ml-1 (P < 0.001). Glucagon was also released on reperfusion after 5 min of ischaemia: mean(s.e.m.) 18(2) fmol ml-1 (P < 0.001 versus control). In a second experiment, pretreatment of rats with allopurinol did not prevent arterial hypotension but abolished ANP release (mean(s.e.m.) 36(2)fmol ml-1 versus no pretreatment 70(7) fmol ml-1, P < 0.05), while glucagon release was unaffected. The release of ANP, but not that of glucagon, is therefore mediated by oxygen free radicals and may signify cardiac and/or pulmonary injury or dysfunction. The actions of these peptides may be relevant in the pathophysiological perturbation of intestinal ischaemia-reperfusion.
Meleagros
L
L
Department of Endocrinology and Metabolism, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Ghatei
M A
MA
Bloom
S R
SR
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood*; D001794:Blood Pressure; D005609:Free Radicals; D005934:Glucagon / Q000097:blood*; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000097:blood*; D008297:Male; D011796:Quinacrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D013997:Time Factors
D005609:Free Radicals; D000493:Allopurinol; D009320:Atrial Natriuretic Factor; D005934:Glucagon; D011796:Quinacrine