title: Sequence analysis of a 3.5 Kb EcoRI fragment from the left arm of Saccharomyces cerevisiae chromosome XI reveals the location of the MBR1 gene and a sequence related to a GTPase-activating protein.
abstract: We present the DNA sequence analysis of a region covering a 3.5 kb EcoRI fragment from the left arm of chromosome XI from Saccharomyces cerevisiae. This region contains five open reading frames (ORFs) which code for proteins of greater than 100 amino acids. ORF YKL425 codes for the previously sequenced Mbr1 (Valens et al., 1991; Daignan-Fornier et al., 1993) which participates in mitochondrial biogenesis. YKL424 has identity with a GTPase-activating protein of higher eukaryotes. The three remaining ORFs have no identity to known proteins within the databases screened and are not assigned ORF numbers as they are completely contained with ORFs YKL424 and YKL425.
authors.affiliation: Manchester Biotechnology Centre, University of Manchester Institute of Science and Technology (UMIST), U.K.
authors.forename: C M
authors.initials: CM
authors.lastname: James
authors.forename: M E
authors.initials: ME
authors.lastname: Gent
authors.forename: S G
authors.initials: SG
authors.lastname: Oliver
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015825:Chromosomes, Fungal; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D020690:GTPase-Activating Proteins; D005800:Genes, Fungal / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; D020690:GTPase-Activating Proteins; C087579:MBR1 protein, S cerevisiae; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins