title: A novel antigen of the dermal-epidermal junction defined by an anti-CD1b monoclonal antibody (NU-T2).
abstract: NU-T2 is a mouse monoclonal IgG1 antibody to the CD1b molecule, (cross-)reacting with an antigen of the dermal-epidermal junction (NU-T2 DEJ AG). Further immunohistochemical characterization of the NU-T2 DEJ AG showed it to display unique properties that differentiate it from other known antigens of the dermal-epidermal junction. Indeed, the NU-T2 DEJ AG is primate-specific and present only in epithelial basement membranes. In normal human skin it is expressed within the lowermost lamina lucida of the dermal-epidermal junction but not in the deep part of epidermal appendages nor in the deep part of epidermal appendages nor in the basement membrane of dermal vessels, smooth muscles or nerves. In diseases with intraepidermal or intradermal cleavage, NU-T2 reactivity was observed at the floor of the blister. In various skin specimens with a cleavage through the lamina lucida (NaCl--or dispase-split skin, bullous pemphigoid, junctional epidermolysis bullosa), NU-T2 immunoreactivity seemed reduced, being localized at the dermal side of the cleavage. These results suggest that the antigen recognized by NU-T2 is a novel component of the lamina lucida of the dermal-epidermal junction, that seems to be important for dermal-epidermal adhesion.
authors.affiliation: Laboratory of Dermatology, INSERM U346, Hôpital Edouard Herriot, Lyon, France.
authors.forename: J
authors.initials: J
authors.lastname: Kanitakis
authors.forename: G
authors.initials: G
authors.lastname: Zambruno
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Roche
authors.forename: E
authors.initials: E
authors.lastname: Berti
authors.forename: D
authors.initials: D
authors.lastname: Schmitt
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D001485:Basement Membrane / Q000276:immunology; D004817:Epidermis / Q000276:immunology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D016253:Microscopy, Immunoelectron; D012867:Skin / Q000276:immunology* / Q000648:ultrastructure; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; C097642:antigen NU-T2 DEJ