Electrospray mass spectrometric characterization of the components of protein mixtures and its application to members of the chemokine family of interleukins.
Electrospray mass spectrometry has been employed to detect and characterize the members of a closely related family of immunologically active proteins (chemokines) produced in cell culture by stimulated fibroblasts. The reliability of the method to produce precise data concerning the relative proportions of proteins present in mixtures was investigated in a model system and found to be satisfactory in a range of 10:1 in concentration.
Kay
I
I
St John's Institute of Dermatology, UMDS, University of London, St Thomas' Hospital, UK.
Hafizi
S
S
Barr
R M
RM
Mallet
A I
AI
Greaves
M W
MW
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D016209:Interleukin-8 / Q000302:isolation & purification; D007378:Interleukins / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry
D016209:Interleukin-8; D007378:Interleukins