title: Physiological modulation of the uptake and fate of glucose in brown adipose tissue.
abstract: Glucose utilization indices (GUI values) and rates of fatty acid synthesis in interscapular brown adipose tissue (IBAT) varied during the diurnal cycle in virgin and late-pregnant rats permitted unrestricted access to food. In virgin rats, peak GUI values and lipogenic rates were observed at the end of the dark (feeding) phase, but were not sustained during the light phase. Whereas peak GUI values were comparable with those observed during re-feeding after 24 h starvation, maximum rates of IBAT fatty acid synthesis in virgin rats during the diurnal cycle were only approx. 25% of those measured during re-feeding after 24 h starvation. Despite hyperphagia, GUI values during the diurnal cycle in late-pregnant rats fed ad libitum were generally lower than those of age-matched virgin controls. The percentage of pyruvate dehydrogenase complex present in the active form (PDHa) was also significantly decreased. Suppression of GUI and PDHa was not parallelled by suppression of fatty acid synthesis. IBAT GUI values in late-pregnant rats during chow re-feeding ad libitum after 24 h starvation were only 25% of those of corresponding virgin controls, and stimulation of fatty acid synthesis was also dramatically attenuated. The suppression of IBAT GUI values after re-feeding in pregnancy was not due to depletion of GLUT 4 protein. The results are discussed in relation to the importance of glucose as a precursor for fatty acid synthesis in IBAT.
authors.affiliation: Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, University of London, U.K.
authors.forename: M C
authors.initials: MC
authors.lastname: Sugden
authors.forename: M J
authors.initials: MJ
authors.lastname: Holness
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D001786:Blood Glucose / Q000032:analysis; D002940:Circadian Rhythm / Q000502:physiology; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4; D006963:Hyperphagia; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009124:Muscle Proteins; D009752:Nutritional Status / Q000502:physiology; D010766:Phosphorylation; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013217:Starvation / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D005227:Fatty Acids; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D011768:Pyruvate Dehydrogenase Complex; C495694:Slc2a4 protein, rat; D005947:Glucose