Sequence analysis of the agaB gene encoding a new beta-agarase from Vibrio sp. strain JT0107.
An agarase gene (agaB) was cloned from genomic DNA of Vibrio sp. strain JT0107. Analysis of the 3200 nucleotide sequence just before the agarase 0107 gene (agaA) which existed in genomic DNA of Vibrio sp. strain JT0107 revealed a putative single open reading frame coding for 955 amino acids. Comparison of the deduced amino acid sequence of AgaB to that of agarase 0107 revealed the existence of partially highly homologous regions. A part of this gene was expressed in Escherichia coli to yield a protein with agarase activity. This is the first report of evidence by genetic analysis that at least two different kinds of agarases exist in strain JT0107.
Sugano
Y
Y
Seawater Science Research Laboratory, Japan Tobacco Inc., Kanagawa.
Matsumoto
T
T
Noma
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics
D004269:DNA, Bacterial; D006026:Glycoside Hydrolases; C019624:agarase