Comparison of two time-resolved fluoroimmunoassays (TR-FIA), as applied to oestriol in human serum and progesterone in bovine milk.
We compared two time-resolved fluoroimmunoassay systems for measuring free oestriol in human serum and progesterone in bovine milk. By reading the fluorescence of europium complex of 4,7-bis(chlorosulphophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid in solution, the measuring range is increased for both oestriol (10-50,000 ng/l instead of 25-50,000 ng/l) and for progesterone (10-50,000 ng/l instead of 25-10,000 ng/l). In addition, the interassay coefficients of variation were lowered from 9.5 to 5.7% for oestriol and from 7.5 to 5.4% for progesterone, at the smaller hormone concentrations detectable by each method.
Ius
A
A
Istituto Chimica Ormoni C. N. R., Milano, Italy.
Iacobello
C
C
Meroni
G
G
Bacigalupo
M A
MA
D000818:Animals; D002417:Cattle; D004964:Estriol / Q000097:blood*; D005456:Fluorescent Dyes; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D008297:Male; D008892:Milk / Q000737:chemistry*; D010618:Phenanthrolines; D011374:Progesterone / Q000032:analysis*
D005456:Fluorescent Dyes; D010618:Phenanthrolines; C054206:4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid; D011374:Progesterone; D004964:Estriol