A baculovirus gene involved in late gene expression predicts a large polypeptide with a conserved motif of RNA polymerases.
We have identified and sequenced a novel baculovirus gene, late expression factor eight gene (lef-8), of Autographa californica nuclear polyhedrosis virus that is necessary for efficient expression from late and very late virus gene promoters in a transient expression assay. The predicted gene product, LEF-8, has a molecular mass of 102 kDa and contains a conserved sequence motif, GXKX4HGQ/NKG, found in DNA-directed RNA polymerases throughout the animal, plant, and microbial kingdoms.
Passarelli
A L
AL
Department of Genetics, University of Georgia, Athens 30602.
Todd
J W
JW
Miller
L K
LK
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins; C087702:late expression factor 8, Baculovirus; D012321:DNA-Directed RNA Polymerases