Protein profiles of Brucella suis and Brucella abortus in isoelectric focusing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
The protein profiles of five field isolates of Brucella suis and four field isolates of B. abortus were examined. Isoelectric focusing of soluble proteins from cell sonicates produced 28 bands common to both species, with a further 10 bands being unique to a species. No intraspecies variation was detected. Sodium dodecyl sulfate extracted proteins were resolved by polyacrylamide gel electrophoresis. The proteins were transferred to nitrocellulose and then stained. These techniques allowed the demonstration of 35 bands which were common to both species, with a further 15 bands which were unique to one of the two species. Again, no intraspecies variation was detected.
Hill
R A
RA
Rockhampton Veterinary Laboratories, Department of Primary Industries, Queensland, Australia.
Cook
D R
DR
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D002002:Brucella / Q000737:chemistry* / Q000145:classification; D002003:Brucella abortus / Q000737:chemistry* / Q000145:classification; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D013552:Swine
D001426:Bacterial Proteins