Epithelial cell coculture and the induction of sperm capacitation.
OBJECTIVE
To investigate the influence of cultured human oviductal epithelial cells on the movement characteristics of human spermatozoa.


METHODS
Human spermatozoa were cultured with monolayers of human epithelial or Vero cells or conditioned media derived from these cell types. The viability and movement characteristics of the cells was subsequently analyzed at 4, 24, and 48 hours.


METHODS
University hospital and Medical Research Council laboratories.


METHODS
Volunteer donors.


METHODS
Movement characteristics of human spermatozoa.


RESULTS
The presence of both Vero and oviductal epithelial cells, but not conditioned media, had a general promoting effect on sperm survival, significantly enhancing sperm viability and motility for up to 48 hours of culture. In addition, the presence of oviductal epithelial cells had a specific, significant stimulatory effect on sperm capacitation, enhancing the incidence of hyperactivated motility after 4, 24, and 48 hours of culture. Significantly, this effect was not observed with cocultures containing Vero cells.


CONCLUSIONS
The coincubation of human spermatozoa with human oviductal epithelial cells provides a convenient system for the induction and analysis of sperm capacitation.
Kervancioglu
M E
ME
Academic Department of Obstetrics and Gynaecology, London Hospital Medical College, United Kingdom.
Djahanbakhch
O
O
Aitken
R J
RJ
D000328:Adult; D002450:Cell Communication; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D005187:Fallopian Tubes / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D013075:Sperm Capacitation / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors