Amplification and analysis of a male-specific SRY homologous sequence of cattle.
Based on the highly conserved region of human, rabbit and mouse SRY (sex-determining region Y) sequences, primers were designed for the amplification of the unknown homologous sequence of cattle by polymerase chain reaction (PCR). A 200-bp male-specific amplifying fragment was obtained. The fragment shares high homology with the known SRY gene. By using the cloned fragment as a probe to hybridize to the Southern blot of HindIII-digested cattle genomic DNA, a 1.7-kb male-specific fragment was detected, which is considered to contain the 200-bp fragment. When the same pair of primers was used in amplification of human and goat DNA samples, the 200-bp male-specific fragment could be detected.
Zhai
W
W
Institute of Genetics, Chinese Academy of Sciences, Beijing.
Chen
X
X
Wu
D
D
Xu
J
J
Gao
Y
Y
Zhu
L
L
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis; D014998:Y Chromosome