Inhibitory effect of neurotensin on proline.
The effect of neurotensin (NT) on proline absorption across rat jejunum was investigated using the single-pass perfusion technique. This study showed that intravenous administration of NT produced a dose-dependent inhibition of proline absorption. Thus, NT at a 0.16 pmol/kg/min concentration gave 10% decrease in proline absorption while 0.32 and 1.6 pmol/kg/min concentration gave 31% and 45% decrease, respectively. In the absence of Na, proline absorption decreased to 77% from control values. No change in proline absorption was noticed when NT at a concentration of 0.32 pmol/kg/min was intravenously injected in the absence of sodium from the perfusion solution. Water absorption did not show significant changes (P > 0.05) in presence or absence of NT. Moreover, NT did not produce a significant change (P > 0.2) in intracellular proline accumulation. NT inhibited proline absorption through an indirect mechanism that is Na-dependent and independent of changes in water absorption.
Abd-Ul-Salam
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HA
Department of Physiology, Faculty of Medicine, American University of Beirut, Lebanon.
Nassar
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CF
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D007408:Intestinal Absorption / Q000187:drug effects*; D007583:Jejunum / Q000166:cytology / Q000187:drug effects*; D009496:Neurotensin / Q000008:administration & dosage / Q000494:pharmacology*; D011392:Proline / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism
D009496:Neurotensin; D011392:Proline; D012964:Sodium