An improved method for rapid fixation and embedding of pteridophyte plant materials.
A rapid method for fixation and embedding of plant materials, especially pteridophytes, is suggested. Addition of tannic acid following osmication improved the visualization of membranes. Staining en bloc with uranyl acetate between osmication and tannic acid is suggested for tissues infected with fungi and bacteria.
Devi
S
S
Electron Microscopy Section, National Botanical Research Institute, Lucknow, India.
Lall
V K
VK
Garg
P
P
Saxena
A
A
Tiwari
M
M
D002101:Cacodylic Acid; D005404:Fixatives; D005976:Glutaral; D009942:Organometallic Compounds; D009993:Osmium Tetroxide; D059828:Plant Cells; D010935:Plant Diseases; D016610:Tissue Embedding; D016707:Tissue Fixation
D005404:Fixatives; D009942:Organometallic Compounds; C005460:uranyl acetate; D002101:Cacodylic Acid; D009993:Osmium Tetroxide; D005976:Glutaral