Presence of mrr- and mcr-like restriction systems in coryneform bacteria.
Efficient transformation of Brevibacterium flavum MJ233C and Corynebacterium glutamicum ATCC 31831 (up to 5.0 x 10(7) transformants/microgram DNA) depends on the source of plasmid DNA. The transformation efficiencies of B. flavum MJ233C and C. glutamicum ATCC 31831 increased nearly 10(3)-fold when plasmid DNA was isolated from the recipient strain itself or from a damdcm Escherichia coli mutant, as compared with DNA passed through a modification-proficient E. coli strain. These results suggest the presence of a methyl-specific restriction system in certain strains of coryneform bacteria. In addition, electroporation conditions were optimized.
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AA
Mitsubishi Petrochemical Co., Ltd, Tsukuba Research Center, Ibaraki, Japan.
Inui
M
M
Kobayashi
M
M
Kurusu
Y
Y
Yukawa
H
H
D001951:Brevibacterium / Q000235:genetics*; D003352:Corynebacterium / Q000235:genetics*; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D066298:In Vitro Techniques; D008745:Methylation; D010957:Plasmids / Q000235:genetics*; D014170:Transformation, Genetic
D015280:DNA Restriction-Modification Enzymes; D004269:DNA, Bacterial