title: Development and trial of a bovine herpesvirus 1-thymidine kinase deletion virus as a vaccine.
abstract: An Australian bovine herpesvirus 1 (BHV1) isolate with a defined (427 base pair) deletion in the protein coding region of the thymidine kinase gene was obtained by standard marker rescue procedures. After selection in the presence of the nucleotide analogue 5'-iodo-deoxy-uridine the virus was analysed by hybridisation with three differential oligonucleotide probes, restriction endonuclease profile studies and DNA sequence analysis. The virus elicited an immune response in recipient animals after either intramuscular or intravenous administration and produced no significant deleterious side-effects when administered at a dose sufficient to stimulate the host immune response. The safety and immunogenicity of the recombinant BHV1 virus 39B1 were similar to those reported for other registered BHV1 vaccines and the virus would appear to be suitable for the production of a vaccine seed lot and more exhaustive field trials as a prelude to commercial vaccine production and registration.
authors.affiliation: Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, University of Queensland.
authors.forename: G A
authors.initials: GA
authors.lastname: Smith
authors.forename: P L
authors.initials: PL
authors.lastname: Young
authors.forename: B J
authors.initials: BJ
authors.lastname: Rodwell
authors.forename: M A
authors.initials: MA
authors.lastname: Kelly
authors.forename: G J
authors.initials: GJ
authors.lastname: Storie
authors.forename: C A
authors.initials: CA
authors.lastname: Farrah
authors.forename: J S
authors.initials: JS
authors.lastname: Mattick
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002417:Cattle; D002872:Chromosome Deletion; D015342:DNA Probes / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D005260:Female; D007242:Herpesvirus 1, Bovine / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D007241:Infectious Bovine Rhinotracheitis / Q000517:prevention & control*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D013937:Thymidine Kinase / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000009:adverse effects / Q000276:immunology; D014765:Viral Vaccines / Q000009:adverse effects / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015342:DNA Probes; D004279:DNA, Viral; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D013937:Thymidine Kinase