Thimet oligopeptidase: similarity to 'soluble angiotensin II-binding protein' and some corrections to the published amino acid sequence of the rat testis enzyme.
The deduced amino acid sequence of pig liver soluble angiotensin II-binding protein [Sugiura, Hagiwara and Hirose (1992) J. Biol. Chem. 267, 18067-18072] is similar over most of its length to that reported for rat testis thimet oligopeptidase (EC 3.4.24.15) by Pierotti, Dong, Glucksman, Orlowski and Roberts [(1990) (Biochemistry 29, 10323-10329]. We have found that homogeneous rat testis thimet oligopeptidase binds angiotensin II with the same distinctive characteristics as the pig liver protein. Analysis of the nucleotide sequences reported for the two proteins pointed to the likelihood that sequencing errors had caused two segments of the amino acid sequence of the rat protein to be translated out of frame, and re-sequencing of selected parts of the clone (kindly provided by the previous authors) confirmed this. The revised deduced amino acid sequence of rat thimet oligopeptidase contains 687 residues, representing a protein of 78,308 Da, and is more closely related to those of the pig liver protein and other known homologues of thimet oligopeptidase than that described previously.
McKie
N
N
Department of Biochemistry, Strangeways Research Laboratory, Cambridge, U.K.
Dando
P M
PM
Rawlings
N D
ND
Barrett
A J
AJ
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats; D011945:Receptors, Angiotensin / Q000235:genetics*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012995:Solubility; D013552:Swine; D013737:Testis / Q000201:enzymology*
D018076:DNA, Complementary; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase