Adenine.methylthymine base-pairs enhance non-uniformity in DNA helices.
Nitroso compounds are known to induce mutations and cancer. Here we study the effect of methylation of O4 of thymine by nitroso compounds on the structure and dynamics of DNA helices. Four dodecamers, for which there exist experimental data obtained by NMR techniques, are studied using very long (approximately 1 ns) molecular dynamics simulations. The conformations obtained are in good agreement with the NMR data. A statistical analysis indicates that DNA in solution adopts conformations which are intermediate between those of the ideal DNA families, such as A and B-DNA. Also, the structures obtained in these molecular dynamics simulations possess a greater degree of non-uniformity than the crystal structures. Most importantly, the helices containing adenine.methylthymine base-pairs show a further enhancement in non-uniformity. A biological role for the enhanced nonuniformity is suggested.
Cruzeiro-Hansson
L
L
Department of Crystallography, Birkbeck College, London, U.K.
Umrania
Y
Y
Goodfellow
J M
JM
D000225:Adenine / Q000737:chemistry*; D000465:Algorithms; D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013941:Thymine / Q000031:analogs & derivatives* / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; C021223:O-4-methylthymine; D004247:DNA; D000225:Adenine; D013941:Thymine