Activity of a maize ubiquitin promoter in transgenic rice.
We have used the maize ubiquitin 1 promoter, first exon and first intron (UBI) for rice (Oryza sativa L. cv. Taipei 309) transformation experiments and studied its expression in transgenic calli and plants. UBI directed significantly higher levels of transient gene expression than other promoter/intron combinations used for rice transformation. We exploited these high levels of expression to identify stable transformants obtained from callus-derived protoplasts co-transfected with two chimeric genes. The genes consisted of UBI fused to the coding regions of the uidA and bar marker genes (UBI:GUS and UBI:BAR). UBI:GUS expression increased in response to thermal stress in both transfected protoplasts and transgenic rice calli. Histochemical localization of GUS activity revealed that UBI was most active in rapidly dividing cells. This promoter is expressed in many, but not all, rice tissues and undergoes important changes in activity during the development of transgenic rice plants.
Cornejo
M J
MJ
Agricultural Research Service, United States Department of Agriculture, Albany, CA 94710.
Luth
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Blankenship
K M
KM
Anderson
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Blechl
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D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005966:Glucuronidase / Q000235:genetics; D006358:Hot Temperature; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015183:Restriction Mapping; D014170:Transformation, Genetic; D014452:Ubiquitins / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D014452:Ubiquitins; D004247:DNA; D005966:Glucuronidase