Collagenase distension, two-step sequential filtration, and histopaque gradient purification for consistent isolation of pure pancreatic islets from the market-age (6-month-old) pig.
Marchetti
P
P
Cattedra di Malattie del Metabolismo, Università di Pisa, Italy.
Giannarelli
Villani
G
G
Andreozzi
M
M
Cruschelli
L
L
Cosimi
S
S
Viacava
P
P
di Carlo
A
A
Navalesi
R
R
Bevilacqua
G
G
D000367:Age Factors; D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D017364:Collagenases; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D013552:Swine
D017364:Collagenases