title: Human peripheral gamma delta T cells are stimulated by Daudi Burkitt's lymphoma and not by any other Burkitt's lymphoma tested.
abstract: Daudi Burkitt's lymphoma cells have molecules on their surface which can stimulate proliferation of human gamma delta T cells, while Raji, another Burkitt's lymphoma, cannot stimulate human gamma delta T cells. Human peripheral gamma delta T cells, coexpressing the V gamma 9/V delta 2 chains of the T cell receptor, lyse Daudi cells but not Raji cells. Here, we have screened four other Burkitt's lymphoma cell lines (HH514, DG75, Ramos, and Wilson), as well as cells derived from a fresh Burkitt's lymphoma, to see if any of them can be recognized by human gamma delta T cells. Leukemia-derived lines MOLT-4, CEM, and K562 have also been included in these studies. Among the Burkitt's lymphomas tested, only Daudi, DG75, and HH514 could be lysed by V gamma 9/V delta 2+ T cell clones derived from the peripheral blood of healthy donors. These T cell clones were also able to lyse the NK sensitive leukemia lines K562 and MOLT-4. When bulk cultures of peripheral blood mononuclear cells from healthy donors were cultured with different Burkitt's lymphoma or leukemia cell lines, only Daudi stimulated the outgrowth of gamma delta T cells. Similarly, only Daudi cells could stimulate proliferation of gamma delta T cell clones, and the response was enhanced significantly in the presence of interleukin-2. These data and our prior observations showing the use of the V gamma 9/V delta 2 TCR type by Daudi-reactive human gamma delta T cells indicate that Daudi cells are not representative of other Burkitt's lymphoma cell lines.
authors.affiliation: Department of Human Oncology, University of Wisconsin, Madison 53792.
authors.forename: I
authors.initials: I
authors.lastname: Kaur
authors.forename: J
authors.initials: J
authors.lastname: de Jong
authors.forename: K
authors.initials: K
authors.lastname: Schell
authors.forename: J
authors.initials: J
authors.lastname: Hank
authors.forename: P
authors.initials: P
authors.lastname: Fisch
authors.forename: P M
authors.initials: PM
authors.lastname: Sondel
mesh_terms: D002051:Burkitt Lymphoma / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta