Effect of nilvadipine on adrenocortical cell proliferation and steroidogenesis in early stage of adrenal regeneration.
The influence of nilvadipine, a novel calcium channel antagonist on adrenal regeneration was investigated in male Wistar rats. The estimation of the mitotic index after administration of colchicine was supplemented by an immunoperoxidase technique with monoclonal antibodies to bromodeoxyuridine. Plasma corticosterone was determined by a radioimmunological assay. Nilvadipine was given subcutaneously in two doses (0.2 and 1 mg/kg) to animals subjected to adrenal enucleation combined with contralateral adrenalectomy. It was found that nilvadipine inhibited the proliferation ratio of adrenocortical cells on the 4th day after surgery in a dose-dependent manner. The inhibitory effect of nilvadipine became less significant by 8 days after operation. However, no changes in corticosterone secretion were observed.
Zieleniewski
W
W
Department of Clinical Endocrinology, University School of Medicine, Lodz, Poland.
D000302:Adrenal Cortex / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003345:Corticosterone / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects*
D002121:Calcium Channel Blockers; C035100:nilvadipine; D009543:Nifedipine; D002118:Calcium; D003345:Corticosterone