NCAM and lung cancer.
The presence of the neural cell adhesion molecule, NCAM, is indicative for a poor prognosis in lung-cancer patients. Using MAb 735, we have investigated the expression of polysialic acid, PSA, on NCAM in a spectrum of neuro-endocrine lung tumors, ranging from the slowly growing typical carcinoids via the atypical carcinoids with clinically unpredictable behavior to the highly aggressive small-cell lung carcinomas. Our immunohistochemical findings indicate a significant association between the presence of PSA on the tumor cells and an aggressive and immature sub-type of the tumor. This might be related to impairment of cell-cell and cell-matrix interactions by the presence of PSA, as we demonstrated in vitro, since detachment is one of the first steps in the metastatic process. The NCAM-MAb 123C3 used in these studies appeared extremely useful in immunoscintigraphy and immunotherapy of SCLC xenografts in nude mice, and for immunoscintigraphy of a SCLC patient. This may be explained by internalization of the 123C3 antibody, which we demonstrated in vitro. 123C3 is the only Cluster-I SCLC MAb studied thus far that becomes internalized.
Michalides
R
R
Department of Tumor Biology, The Netherlands Cancer Institute, Amsterdam.
Kwa
B
B
Springall
D
D
van Zandwijk
N
N
Koopman
J
J
Hilkens
J
J
Mooi
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D002276:Carcinoid Tumor / Q000000981:diagnostic imaging / Q000473:pathology; D018288:Carcinoma, Small Cell / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007457:Iodine Radioisotopes / Q000627:therapeutic use; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology / Q000556:secondary*; D008175:Lung Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology* / Q000532:radiotherapy / Q000601:surgery; D051379:Mice; D008819:Mice, Nude; D018358:Neuroendocrine Tumors / Q000000981:diagnostic imaging / Q000473:pathology* / Q000532:radiotherapy / Q000601:surgery; D011379:Prognosis; D016499:Radioimmunotherapy; D011877:Radionuclide Imaging; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D007457:Iodine Radioisotopes