Clonal analysis for developmental potential of chick periosteum-derived cells: agar gel culture system.
The developmental potential of periosteum-derived cells was clonally assessed with an agar gel culture system. Morphologically, two types of colonies were predominantly observed. By immunocytochemical observation with antibodies against aggrecan or bone Gla protein, one type of colony was judged to be chondrogenic, and the other osteogenic. By chronological observation, each type of colony did not convert to the other. Supplementation with transforming growth factor (TGF)-beta 1 shortened the time course of chondrogenesis and also increased colony forming efficiency of chondrogenic colonies. On the other hand, colony forming efficiency of osteogenic colonies decreased with TGF-beta 1 treatment, whereas the time course of osteogenesis remained unaffected. These observations suggest that there are both committed osteoprogenitor and chondroprogenitor cells present in the periosteal cell population, and TGF-beta 1 stimulates proliferation and differentiation of chondrogenic cell population by its targeted action.
Nakase
T
T
Department of Orthopaedic Surgery, Osaka University Medical School, Suita, Japan.
Nakahara
H
H
Iwasaki
M
M
Kimura
T
T
Kimata
K
K
Watanabe
K
K
Caplan
A I
AI
Ono
K
K
D000362:Agar; D053674:Aggrecans; D000818:Animals; D002356:Cartilage / Q000254:growth & development; D002645:Chickens; D002999:Clone Cells; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D016326:Extracellular Matrix Proteins; D007150:Immunohistochemistry; D037181:Lectins, C-Type; D015675:Osteocalcin / Q000302:isolation & purification; D010521:Periosteum / Q000166:cytology* / Q000254:growth & development*; D011509:Proteoglycans / Q000302:isolation & purification; D013234:Stem Cells / Q000187:drug effects; D013977:Tibia / Q000166:cytology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D053674:Aggrecans; D003470:Culture Media; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; D000362:Agar