title: Interaction of tocopherol with peroxyl radicals does not lead to the formation of lipid hydroperoxides in liposomes.
abstract: alpha-Tocopherol (alpha-TOH) is thought to act as a lipid-soluble antioxidant by scavenging the chain-carrying lipid peroxyl radical (LOO.), giving rise to alpha-TO. and LOOH. The aim of the present work is to test the possibility that alpha-TOH may also exert a protective action by converting LOO. into the parent, unoxidized lipid molecule (LH) with concomitant formation of O2- and T+O. To assess the relative contribution of the two pathways, the amounts of products accumulating were measured, i.e. LOOH and O2-, under conditions where lipid peroxidation was initiated at linear rates by the UV light-induced decomposition of AIBN. The overall conclusion of the study is based on the observation that significant amounts of O2- were produced, whereas the delta LOOH/delta alpha-TOH was considerably smaller than 1.0. A mechanism of one or two-electron oxidation of TOH is considered; according to it the phospholipid primary structure is restored (LOO.-->LH, breaking of the C-->O bond). This leads to the oxygen or superoxide anion-radical release accordingly.
authors.affiliation: Group of Lipid Biochemistry, Cardiology Research Centre, Moscow, Russia.
authors.forename: L F
authors.initials: LF
authors.lastname: Dmitriev
authors.forename: M V
authors.initials: MV
authors.lastname: Ivanova
authors.forename: V Z
authors.initials: VZ
authors.lastname: Lankin
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003574:Cytochrome c Group / Q000737:chemistry; D016166:Free Radical Scavengers; D005609:Free Radicals; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D010084:Oxidation-Reduction; D010545:Peroxides / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry; D014810:Vitamin E / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D003574:Cytochrome c Group; D016166:Free Radical Scavengers; D005609:Free Radicals; D008054:Lipid Peroxides; D008081:Liposomes; D010545:Peroxides; D013481:Superoxides; D014810:Vitamin E; C049375:perhydroxyl radical