A method for accurate amplification of polymorphic CA-repeat sequences.
Anomalous PCR products are often produced during the amplification of d(CA)n.d(TG)n sequences. Upon denaturing polyacrylamide gel electrophoresis, these products yield a ladder-like pattern that can complicate genotypic interpretation. We have developed two related techniques, referred to as two- and three-stage linear amplification (2-SLA and 3-SLA, respectively), which largely overcome this problem and yield readily interpretable banding patterns.
Odelberg
S J
SJ
Department of Human Genetics, University of Utah, Salt Lake City 84112.
White
R
R
D000225:Adenine; D000483:Alleles; D001483:Base Sequence; D003596:Cytosine; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D005819:Genetic Markers; D003596:Cytosine; D004247:DNA; D000225:Adenine