Quinoxalinone derivatization of biological carboxylic acids for detection by peroxyoxalate chemiluminescence with high-performance liquid chromatography.
A quinoxalinone fluorescent tag is evaluated as a carboxylic acid derivatizing reagent for detection by peroxyoxalate chemiluminescence. The synthetic procedure for the quinoxalinone was modified to give a yield that is significantly increased over that reported previously. The new conditions use less hazardous reagents, and produce a final product greater than 97% pure, without the need for intermediate clean-up steps. The derivatization reaction is also modified to give increased yields of greater than 85% compared to 74% obtained previously. The post-column chemiluminescence reaction conditions are optimized to give detection limits of 500 attomole/injection-10 times lower than the fluorescence previously obtained. The reagent is used for the first time on a plasma sample extract. Typical method precision is 4%.
Sandmann
B W
BW
Department of Chemistry, Seton Hall University, South Orange, NJ 07079.
Grayeski
M L
ML
D000818:Animals; D002264:Carboxylic Acids / Q000032:analysis* / Q000737:chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes; D006834:Hydrazines / Q000737:chemistry*; D008163:Luminescent Measurements; D010070:Oxalates / Q000737:chemistry*; D011810:Quinoxalines / Q000737:chemistry*; D015203:Reproducibility of Results; D014635:Valproic Acid / Q000032:analysis / Q000097:blood
D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D006834:Hydrazines; D010070:Oxalates; D011810:Quinoxalines; C087646:6,7-dimethoxy-1-methyl-2(1H)-quinoxalinone-3-proprionylcarboxylic acid hydrazide; C044622:peroxyoxalate; D014635:Valproic Acid