Method for efficient pelleting of small unilamellar model membrane vesicles.
Separation of model membrane vesicles from external solution is an important step in quantification of vesicle-associated materials. Ordinarily, such experiments cannot be done with small unilamellar vesicles because they are too small to be pelleted by centrifugation. A method is described that overcomes this difficulty by use of brominated phospholipids. It is shown that efficient pelleting of small unilamellar vesicles (greater than 98%) can be obtained by ultracentrifugation.
Tortorella
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Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794-5215.
London
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D001966:Bromine / Q000737:chemistry*; D002498:Centrifugation / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008954:Models, Biological; D010713:Phosphatidylcholines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D012680:Sensitivity and Specificity
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010743:Phospholipids; C049875:1,2-di(9,10-dibromostearoyl)phosphatidylcholine; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; D001966:Bromine