Comparison of the cytotoxic activity of the immunotoxins with different internalization rate.
In comparative experiments the conjugates of A-chain of a plant toxin ricin (RTA) and monoclonal antibody (MAb) HAE9 (IgM) directed against human erythroblast antigen (Ag-Eb) or Mab HAE3 against human glycophorin-A and immunotoxins (IT) directed to CD5 and CD7 antigens of human T-lymphocytes have been investigated. Proceeding from the number of receptors on a cell, we compared efficiency of the cytotoxic effects of the conjugates with different internalization rate. The efficiency of immunoconjugates against Ags with an extremely high internalization rate was only slightly higher than that of immunoconjugates against Ags with a lower internalization rate. The enhancing effect of ammonium chloride on immunoconjugate cytotoxicity was more pronounced in the case of immunotoxins with a high internalization rate.
Tonevitsky
A G
AG
Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
Agapov
I I
II
Mechetner
E B
EB
Ershova
G V
GV
Toptygin AYu
Sarma
T
T
Shamshiev
A T
AT
Pfueller
U
U
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000187:drug effects; D001665:Binding Sites; D002470:Cell Survival / Q000187:drug effects*; D004900:Erythroblasts / Q000187:drug effects; D006801:Humans; D000922:Immunotoxins / Q000378:metabolism / Q000633:toxicity*; D011500:Protein Synthesis Inhibitors / Q000633:toxicity; D012276:Ricin / Q000493:pharmacokinetics / Q000633:toxicity*; D013601:T-Lymphocytes / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D011500:Protein Synthesis Inhibitors; D012276:Ricin