Selection and micro-injection of acrosome-reacted human spermatozoa.
A method of selection of acrosome-reacted human spermatozoa is described. Petri dishes were coated with GB24 antibody, specific to the inner acrosomal membrane. The acrosome-reacted spermatozoa were fixed on the antibody and could be removed by aspiration with a micro-pipette. They were then injected into the perivitelline space of hamster eggs in order to check their fertilizing ability. This selection allowed the fertilization rate to be significantly increased (24 versus 7% for control spermatozoa; P < 0.01). This method could enhance the results of human sub-zonal insemination.
Parinaud
J
J
Centre de Fécondation In Vitro, CHU La Grave, France.
Vieitez
G
G
Labal
B
B
Richoilley
G
G
D000177:Acrosome / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D006224:Cricetinae; D005260:Female; D005306:Fertilization / Q000502:physiology*; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods*; D008297:Male; D008845:Microinjections
D000911:Antibodies, Monoclonal