Ubiquitous factors that interact simultaneously with two distinct cis-elements on the rat aldolase B gene promoter.
The proximal promoter region (nucleotide -202 to -1) of the rat aldolase B gene confers liver-specific transcription, and contains three indispensable protein-binding sites (site A, site B and site C). Site A binds HNF-1 and HNF-3, and site B binds NF-Y and a CCAAT-binding factor AlF-B (Tsutsumi et al. (1989) Mol. Cell. Biol. 9, 4923-4931; Raymondjean et al. (1991) Nucleic Acids Res. 19, 6145-6153), trans-acting factors that interact with site C, however, have not been well characterized. In this study, we identified specific factors that bind site C by two-dimensional gel electrophoretic analyses. The factors interacted with two distinct cis-elements; site C and site B. This observation, together with the fact that site C contains a C/EBP binding motif, implied that the site C-binding factors are members of C/EBP family. However, analyses of their binding characteristics, their relative molecular masses, and their distribution in different cell types showed that the site C binding factors are different from known members of C/EBP family.
Yabuki
T
T
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Japan.
Ejiri
S
S
Tsutsumi
K
K
D000418:Albumins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry / Q000235:genetics*; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D015534:Trans-Activators / Q000302:isolation & purification* / Q000378:metabolism
D000418:Albumins; D015534:Trans-Activators; D005634:Fructose-Bisphosphate Aldolase