Protein C activation by an activator purified from the venom of Agkistrodon halys halys.
The protein C activator from Agkistrodon halys halys venom was purified 533-fold by ion-exchange chromatography on QAE-Sephadex A-50, affinity chromatography on aprotinin-Sepharose and Mono-Q fast protein liquid chromatography. The purified enzyme is a single chain protein with an apparent molecular weight of 36,000 that activates protein C by proteolytic removal of a small fragment from the heavy chain. The protein C activator exhibited a high amidolytic activity towards the tripeptide substrates D-Pro-Phe-Arg-pNA (S2302) and D-Phe-(pipecolyl)-Arg-pNA (S2238). The activity of the activator was not affected by thiolprotease or metalloprotease inhibitors. The activator was inhibited, however, by benzamidine, Phe-Pro-Arg chloromethyl ketone, p-nitrophenyl p-guanidinobenzoate and soy bean trypsin inhibitor, which classifies the enzyme as a serine protease. The purified protease was capable of activating both human and bovine protein C. Activation of human protein C only occurred at an appreciable rate in a calcium-free reaction medium at low ionic strength. Ca2+ ions inhibited the activation of human protein C with an apparent Ki of 0.8 mM. Addition of NaCl to the reaction medium also strongly inhibited human protein C activation (50% inhibition at 20 mM NaCl). Kinetic analysis of human protein C activation by the venom activator (in a calcium-free medium) revealed an apparent Km for protein C of 0.52 microM and a kcat of 0.17 s-1 at I = 0.05 (kcat/Km = 3.3 x 10(5) M-1 s-1). At I = 0.15 rates of human protein C activation became linear with protein C indicating a strong increase in Km with increasing ionic strength. Activation of bovine protein C was hardly affected by variation of Ca2+ and NaCl concentrations in the reaction medium. The apparent Kis for calcium ion and NaCl inhibition of bovine protein C activation were > 10 mM and 220 mM, respectively. At I = 0.1 and in the absence of Ca2+ ions bovine protein C was activated with a Km of 0.056 microM and a kcat of 0.24 s-1 (kcat/Km = 4.3 x 10(6) M-1 s-1). Our data are indicative for a rather large conformational and/or structural difference between human and bovine protein C at physiological ionic strength.
Bakker
H M
HM
Cardiovascular Research Institute Maastricht, University of Limburg, The Netherlands.
Tans
G
G
Yukelson
L Y
LY
Janssen-Claessen
T W
TW
Bertina
R M
RM
Hemker
H C
HC
Rosing
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002417:Cattle; D002863:Chromogenic Compounds / Q000378:metabolism; D003435:Crotalid Venoms / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D011486:Protein C / Q000187:drug effects*; D012965:Sodium Chloride / Q000494:pharmacology
D002863:Chromogenic Compounds; D003435:Crotalid Venoms; D011486:Protein C; D012965:Sodium Chloride; D002122:Calcium Chloride