title: The effect of trans-stifle external skeletal fixation and hyaluronic acid therapy on articular cartilage in the dog.
abstract: Transarticular external skeletal (TES) fixators were applied unilaterally to the stifle joints of 10 young adult dogs. After 4 weeks, the fixators were removed from all dogs. Two dogs were not allowed a remobilization period, whereas 8 dogs were provided with 4 additional weeks of weight-bearing activity in a kennel run. Four dogs were given high-molecular weight hyaluronic acid by intra-articular injection weekly during the remobilization period. Clinical gait evaluations and range of motion were determined during the remobilization period. Articular cartilage samples from both stifle joints of all dogs were evaluated histologically and histochemically. No significant differences in gait scores or range of motion were noted between treated and untreated dogs. Articular cartilage proteoglycan content was reduced after 4 weeks of trans-stifle external skeletal fixation as determined by loss of alcian blue (AB) histochemical staining. Improved homogeneity of histochemical staining was observed after remobilization. However, remobilization was associated with histological damage to the surface and tangential layers of articular cartilage. Remobilization combined with hyaluronic acid (HA) therapy improved histochemical staining and reduced structural damage to articular cartilage when compared with remobilization alone.
authors.affiliation: Department of Small Animal Medicine, Avian Medicine, Anatomy and Radiology, College of Veterinary Medicine, University of Georgia, Athens.
authors.forename: W G
authors.initials: WG
authors.lastname: Keller
authors.forename: D N
authors.initials: DN
authors.lastname: Aron
authors.forename: G N
authors.initials: GN
authors.lastname: Rowland
authors.forename: S
authors.initials: S
authors.lastname: Odend'hal
authors.forename: J
authors.initials: J
authors.lastname: Brown
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry / Q000187:drug effects / Q000473:pathology*; D004285:Dogs / Q000601:surgery*; D016267:External Fixators / Q000009:adverse effects / Q000662:veterinary*; D006651:Histocytochemistry; D006820:Hyaluronic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D007103:Immobilization; D008297:Male; D011509:Proteoglycans / Q000032:analysis / Q000378:metabolism; D011897:Random Allocation; D013264:Stifle
chemical_list: D011509:Proteoglycans; D006820:Hyaluronic Acid