title: Distinct cellular localization of mRNAs for three subtypes of prostaglandin E receptor in kidney.
abstract: Distribution of the mRNAs for three subtypes of prostaglandin E (PGE) receptors in the mouse kidney was investigated by in situ hybridization. The mRNA for EP1 subtype, which is coupled to Ca2+ mobilization, was specifically localized to the collecting ducts from the cortex to the papilla. The mRNA for EP2 subtype, which is linked to stimulation of adenylate cyclase, was localized to the glomeruli. The mRNA for EP3 subtype, which is coupled to inhibition of adenylate cyclase, was located densely in the tubules in the outer medulla and in the distal tubules in the cortex. These results exhibit distinct cellular localization of three subtypes of PGE receptor in the kidney and suggest that PGE2 exerts multiple functions via these subtypes expressed in different segments of the nephron.
authors.affiliation: Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: T
authors.initials: T
authors.lastname: Namba
authors.forename: R
authors.initials: R
authors.lastname: Shigemoto
authors.forename: M
authors.initials: M
authors.lastname: Negishi
authors.forename: A
authors.initials: A
authors.lastname: Ichikawa
authors.forename: S
authors.initials: S
authors.lastname: Narumiya
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D016375:Antisense Elements (Genetics); D001345:Autoradiography; D015232:Dinoprostone / Q000378:metabolism / Q000502:physiology; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009399:Nephrons / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D018078:Receptors, Prostaglandin E / Q000032:analysis / Q000096:biosynthesis*; D013462:Sulfur Radioisotopes
chemical_list: D016375:Antisense Elements (Genetics); D015347:RNA Probes; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E; D013462:Sulfur Radioisotopes; D000262:Adenylyl Cyclases; D015232:Dinoprostone