title: Circadian oscillation of 64-kDa polypeptide in the rat suprachiasmatic nucleus.
abstract: In cell suspensions of suprachiasmatic nucleus harvested every 3 hr from rats kept under 12 hr: 12 hr light-dark cycle and constant darkness, we have detected a M(r) 64-kDa protein whose synthesis exhibits two distinct daily peaks in SDS-PAGE. Analysis of densitometer tracings revealed that the synthesis of other proteins was independent of the time of day or not reproducible. Maximum synthesis of the 64-kDa polypeptide occurred at around CT6 and CT21, which are almost coincident with the phase advance regions of circadian activity rhythm induced by anisomycin and light pulses [15], respectively. These results suggest that the 64-kDa protein in SDS-PAGE may be a part of the circadian clock mechanism.
authors.affiliation: Department of Veterinary Physiology, Faculty of Agriculture, Miyazaki University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Nishi
authors.forename: N
authors.initials: N
authors.lastname: Murakami
authors.forename: N
authors.initials: N
authors.lastname: Marumoto
authors.forename: T
authors.initials: T
authors.lastname: Nasu
authors.forename: H
authors.initials: H
authors.lastname: Kuroda
authors.forename: T
authors.initials: T
authors.lastname: Etoh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002940:Circadian Rhythm; D003624:Darkness; D066298:In Vitro Techniques; D008027:Light; D008297:Male; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013493:Suprachiasmatic Nucleus / Q000378:metabolism* / Q000502:physiology
chemical_list: D009419:Nerve Tissue Proteins; D008715:Methionine