Method for the direct specific measurement of vitamin B12 bound to transcobalamin II in plasma.
The concentration of plasma vitamin B12 bound to transcobalamin II was measured by two methods: QUSO and Heparin Sepharose (HS). The new HS procedure provided a specific, direct measurement of vitamin B12 bound to both R binder and transcobalamin II in plasma.
Benhayoun
S
S
INSERM U308, Department of Biochemistry-Immunology, School of Medicine, Vandoeuvre, France.
Adjalla
C
C
Nicolas
J P
JP
Guéant
J L
JL
Lambert
D
D
D003037:Cobalt Radioisotopes; D006801:Humans; D007201:Indicator Dilution Techniques; D007202:Indicators and Reagents; D011485:Protein Binding; D012685:Sepharose / Q000031:analogs & derivatives; D012822:Silicon Dioxide; D014155:Transcobalamins / Q000378:metabolism*; D014805:Vitamin B 12 / Q000097:blood*
D003037:Cobalt Radioisotopes; D007202:Indicators and Reagents; D014155:Transcobalamins; C037405:heparin-sepharose; D012822:Silicon Dioxide; D012685:Sepharose; D014805:Vitamin B 12