title: Dynamics of Na,K-ATPase sites in lateral cochlear wall tissues of the rat.
abstract: The objective of this study was to determine whether varying levels of either the synthetic glucocorticoid, dexamethasone, or the mineralocorticoid, aldosterone, modulate the quantity of Na,K-ATPase sites in stria vascularis and spiral ligament tissues. Surgically adrenalectomized male rats were administered different dosages of dexamethasone or aldosterone for 7 days and subsequently were sacrificed. Na,K-ATPase alpha-subunits of stria vascularis and spiral ligament homogenates were determined quantitatively by enzyme-linked immunosorbent assay, using a monoclonal antibody shown to react with lateral wall Na,K-ATPase alpha-subunit. Elevated serum levels of dexamethasone were correlated with significantly increased Na,K-ATPase alpha-subunit levels in both the stria vascularis and spiral ligament (P < 0.05). Elevated serum levels of aldosterone were correlated with increased Na,K-ATPase alpha-subunits in the stria vascularis, but not in the spiral ligament (P < 0.1). These data further indicate a positive correlation between increased serum levels of adrenal steroids and induction of Na,K-ATPase synthesis by such steroids, particularly by dexamethasone.
authors.affiliation: Department of Anatomy, College of Medicine, University of Florida, Gainesville 32610.
authors.forename: L M
authors.initials: LM
authors.lastname: Curtis
authors.forename: W J
authors.initials: WJ
authors.lastname: ten Cate
authors.forename: K E
authors.initials: KE
authors.lastname: Rarey
mesh_terms: D000315:Adrenalectomy; D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D003051:Cochlea / Q000201:enzymology*; D003907:Dexamethasone / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis*; D013316:Stria Vascularis / Q000201:enzymology
chemical_list: D000450:Aldosterone; D003907:Dexamethasone; D000254:Sodium-Potassium-Exchanging ATPase