Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis.
A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 micrograms/g) into female lampreys undergoing final maturation before spawning produced a significant (P < 0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.
Conlon
J M
JM
Department of Biomedical Sciences, Creighton University Medical School, Omaha, NE 68178.
Balasubramaniam
A
A
Sower
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D007798:Lampreys / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D011863:Radioimmunoassay
D009478:Neuropeptide Y; D004958:Estradiol