Retention behavior of conjugated metabolites of vitamin D and related compounds in high-performance liquid chromatography.
The retention behavior of sulfates or glucuronides of provitamin D, vitamin D, and 25-hydroxyvitamin D3, together with its fluorescent derivatives, are examined with reversed-phase high-performance liquid chromatography. Inclusion chromatography using cyclodextrin as a mobile-phase additive is also used for this purpose. Conventional reversed-phase high-performance liquid chromatography clearly separates the positionally isomeric conjugates of 25-hydroxyvitamin D3. The addition of the host compound to the mobile phase is effective in separating the pairs of fluorescent derivatives of vitamin-D3 and -D2 conjugates or provitamin-D3 and -D2 conjugates.
Shimada
K
K
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Mitamura
K
K
Kaji
H
H
Morita
M
M
D002112:Calcifediol / Q000302:isolation & purification; D002762:Cholecalciferol / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004872:Ergocalciferols / Q000302:isolation & purification; D005965:Glucuronates / Q000302:isolation & purification; D013431:Sulfates / Q000302:isolation & purification; D014807:Vitamin D / Q000302:isolation & purification*
D004872:Ergocalciferols; D005965:Glucuronates; D013431:Sulfates; D014807:Vitamin D; D002762:Cholecalciferol; D002112:Calcifediol