Casein and tyrosine kinase activities of developing chick embryo liver. Effect of triiodothyronine.
Cytosol cAMP-independent quercetin-inhibited protein kinase of developing chick embryo liver was measured at three embryonic ages (days 12, 14 and 18) in the presence of casein and poly (Glu-Na, Tyr) 4:1 as substrates. In the early embryonic stages the tyrosine kinase was almost as active as casein kinase, but on day 18 the tyrosine phosphorylation was only 25% of the casein phosphorylation. Both kinase activities strongly increased by the end of embryonic development: 7-fold with casein and 2.6-fold with poly (Glu-Na, Tyr) 4:1. Triiodothyronine caused twice the stimulation of casein and tyrosine phosphorylation on day 12, but had no effect on day 18.
Gavrilova
N J
NJ
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
Setchenska
M S
MS
D000818:Animals; D047388:Casein Kinases; D002364:Caseins / Q000378:metabolism; D002642:Chick Embryo; D000242:Cyclic AMP / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000187:drug effects* / Q000196:embryology / Q000201:enzymology*; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology*
D002364:Caseins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014284:Triiodothyronine; C027606:L-glutamic acid-L-tyrosine copolymer; D000242:Cyclic AMP; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D047388:Casein Kinases