title: Fluorescence-activated cell sorting-derived clones of Babesia bigemina show karyotype polymorphism.
abstract: Use of the fluorescence-activated cell sorter proved to be an accurate and highly efficient means for cloning Babesia parasites. These qualities were examined by separating a mixed population of Babesia-infected bovine erythrocytes composed of two isolates with different karyotypes. Direct evidence of polymorphism was detected during comparison of the resultant clones.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
authors.forename: D M
authors.initials: DM
authors.lastname: Estes
authors.forename: C W
authors.initials: CW
authors.lastname: Bailey
authors.forename: L
authors.initials: L
authors.lastname: Barnett
authors.forename: D
authors.initials: D
authors.lastname: Lafrenz
authors.forename: H M
authors.initials: HM
authors.lastname: Brandt
authors.forename: J B
authors.initials: JB
authors.lastname: Jensen
authors.forename: G K
authors.initials: GK
authors.lastname: Allen
authors.forename: C A
authors.initials: CA
authors.lastname: Carson
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D016172:DNA Fingerprinting; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry / Q000379:methods; D007621:Karyotyping; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan