PCR amplification of antibody variable regions using primers that anneal to constant regions.
Sassano
M
M
Tecnogen S.c.p.A Molecular Immunology and Biochemistry Unit, Località La Fagianeria, Piana di Monte Verna, Caserta, Italy.
Repetto
M
M
Cassani
G
G
Corti
A
A
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D017931:DNA Primers; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D007127:Immunoglobulin Constant Regions; D007135:Immunoglobulin Variable Region; D004247:DNA