Molecular cloning of cDNA encoding human drebrin E and chromosomal mapping of its gene.
Drebrins are novel actin-binding proteins in the brain which are developmentally regulated. Three isoforms: two embryonic types (E1 and E2) and an adult type (A) are generated by alternative RNA splicing from a single debrin gene in the chicken brain. A full length cDNA clone of human drebrin E has been isolated from a cDNA library of human fetus brain. The clone is 2596 base pairs in length and contains an open reading frame of 1947 nucleotides encoding a protein of 649 amino acids. The deduced amino acid sequence, except for the internal 138-nucleotide sequence (ins2), exhibits 88% homology with rat drebrin A. Spot blot hybridization using flow-sorted human chromosomes provides evidence that the gene encoding human drebrin protein locates on human chromosome 5.
Toda
M
M
Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
Shirao
T
T
Minoshima
S
S
Shimizu
N
N
Toya
S
S
Uyemura
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000502:physiology; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005333:Fetus; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009479:Neuropeptides; C083795:drebrin E; C053386:drebrins