Evaluation of the osteogenic potential of biomaterials implanted in the palatal connective tissue of miniature pigs using undecalcified sections.
Calcium phosphate or calcium carbonate biomaterials are widely used as bone substitutes in periodontal surgery. This study evaluates the osteogenic potential of five different alloplastic biomaterials implanted in the connective tissue of the palatal papilla in miniature pigs. A porous hydroxyapatite (PHA), a dense hydroxyapatite (DHA), a semi-porous hydroxyapatite (SPHA), a tricalcium phosphate (TCP) and a calcium carbonate natural coral (NC) were implanted in a tunnel in the palatal papillae of seven miniature pigs. Undecalcified sections were examined histologically at 1, 2, 3, 4, 8, 12 and 24 wk intervals. Resorbable materials (TCP and NC) were totally resorbed by 24 wk. DHA, PHA and HA showed very limited resorption, although there were multinucleated giant cells in contact with PHA and SPHA. There was no histologically detectable bone formation in contact with or near any of the biomaterials tested. However, several particles of NC, and sometimes of PHA, were surrounded by a dense, mineralized matrix. It is concluded that none of these biomaterials, in their presently available forms, has any bone inducing capacity.
Naaman Bou-Abboud
N
N
Department of Periodontology, School of Dentistry, Paris, France.
Patat
J L
JL
Guillemin
G
G
Issahakian
S
S
Forest
N
N
Ouhayoun
J P
JP
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001857:Bone Matrix / Q000187:drug effects; D001862:Bone Resorption / Q000139:chemically induced; D002119:Calcium Carbonate / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D003094:Collagen; D003238:Connective Tissue / Q000187:drug effects* / Q000502:physiology; D003239:Connective Tissue Cells; D017886:Durapatite / Q000494:pharmacology; D015726:Giant Cells / Q000187:drug effects; D006644:Histiocytes / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D010159:Palate; D019736:Prostheses and Implants; D013552:Swine; D013556:Swine, Miniature
D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D003094:Collagen; D017886:Durapatite; C020243:calcium phosphate; D002119:Calcium Carbonate; C485829:calcium phosphate, dibasic, anhydrous