The transforming growth factor beta type II receptor can replace the activin type II receptor in inducing mesoderm.
The type II receptors for the polypeptide growth factors transforming growth factor beta (TGF-beta) and activin belong to a new family of predicted serine/threonine protein kinases. In Xenopus embryos, the biological effects of activin and TGF-beta 1 are strikingly different; activin induces a full range of mesodermal cell types in the animal cap assay, while TGF-beta 1 has no effects, presumably because of the lack of functional TGF-beta receptors. In order to assess the biological activities of exogenously added TGF-beta 1, RNA encoding the TGF-beta type II receptor was introduced into Xenopus embryos. In animal caps from these embryos, TGF-beta 1 and activin show similar potencies for induction of mesoderm-specific mRNAs, and both elicit the same types of mesodermal tissues. In addition, the response of animal caps to TGF-beta 1, as well as to activin, is blocked by a dominant inhibitory ras mutant, p21(Asn-17)Ha-ras. These results indicate that the activin and TGF-beta type II receptors can couple to similar signalling pathways and that the biological specificities of these growth factors lie in their different ligand-binding domains and in different competences of the responding cells.
Bhushan
A
A
Molecular Neurobiology Laboratory, Salk Institute, La Jolla, California 92037.
Lin
H Y
HY
Lodish
H F
HF
Kintner
C R
CR
D029404:Activin Receptors; D028341:Activins; D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D007265:Inhibins / Q000494:pharmacology*; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000096:biosynthesis / Q000502:physiology*; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis / Q000502:physiology*; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014982:Xenopus laevis / Q000196:embryology*
D017978:Receptors, Growth Factor; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D028341:Activins; D007265:Inhibins; D017346:Protein Serine-Threonine Kinases; D029404:Activin Receptors; D016283:Proto-Oncogene Proteins p21(ras)