Determination of polychlorinated biphenyl levels in the serum of residents and in the homogenates of seafood from the New Bedford, Massachusetts, area: a comparison of exposure sources through pattern recognition techniques.
We measured the residues of polychlorinated biphenyls (PCBs) in the serum of 23 residents of the New Bedford, Massachusetts, area and from two homogenates each of bluefish and lobsters from the same area. We used congener-specific and total Aroclor quantitative approaches, both of which involved gas chromatography with electron capture detection. Using gas chromatography mass spectrometry (electron ionization mode), we confirmed the presence of PCBs in the combined serum samples and in the aliquots of bluefish and lobsters. In measuring the PCB levels in serum, we found good agreement between the two electron capture detector approaches (r > or = 0.97) when the serum of specific congeners was compared to total Aroclor. We used univariate and multivariate quality control approaches to monitor these analyses. Analytical results for bluefish showed a better agreement between the two techniques than did those for lobsters; however, the small number of samples precluded any statistical comparison. We also measured levels of chlorinated pesticides in the serum samples of two groups of New Bedford residents, those with low PCB levels (< 15 ng/ml) and those with high PCB levels (> or = 15 ng/ml). We found that residents with high PCB levels also tended to have higher levels of hexachlorobenzene (HCB) and 1,1-dichloro-2,2-di-(p-chlorophenyl) ethylene (p,p'-DDE). The higher concentration of all three analytes appears to be influenced by employment in the capacitor industry, by seafood consumption, or both. Using Jaccard measures of similarity and principal component analysis we compared the gas chromatographic patterns of PCBs found in the serum of New Bedford area residents with high serum PCBs with the patterns found in homogenates of lobsters (inclusive of all edible portions except the roe), in homogenates of bluefish fillets taken from local waters, and in serum from goats fed selected technical Aroclors (e.g. Aroclors 1016, 1242, 1254, or 1260). The patterns found in human serum samples were similar to the patterns found in lobster homogenates. Both of these patterns closely resembled patterns found in the serum samples of the goat fed aroclor 1254, as demonstrated by both pattern recognition techniques. In addition, the chromatographic patterns of human serum and of lobsters and bluefish homogenates all indicated the presence of PCBs more characteristic of Aroclors 1016 or 1242.
Burse
V W
VW
Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.
Groce
D F
DF
Caudill
S P
SP
Korver
M P
MP
Phillips
D L
DL
McClure
P C
PC
Lapeza
C R
CR
Head
S L
SL
Miller
D T
DT
Buckley
D J
DJ
D000704:Analysis of Variance; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D007306:Insecticides / Q000032:analysis; D008404:Massachusetts; D008121:Nephropidae / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D011786:Quality Control; D011795:Surveys and Questionnaires
D007306:Insecticides; D011078:Polychlorinated Biphenyls