Malonyl-CoA metabolism in cardiac myocytes and its relevance to the control of fatty acid oxidation.
1. Viable myocytes were obtained from rat hearts. Oxidation of [1-14C]palmitate by these cells could be decreased by the addition of glucose (5 mM) or lactate (2 mM). In the presence of glucose, insulin decreased and adrenaline increased palmitate oxidation. 2. The myocytes contained activities of ATP citrate-lyase, acetyl-CoA carboxylase and the condensing enzyme of the fatty acid elongation system. No fatty acid synthase activity was demonstrable in myocytes. 3. In rat hearts perfused with 5 mM glucose, malonyl-CoA content was acutely raised by insulin. In the presence of glucose+insulin, perfusion with palmitate or adrenaline decreased the malonyl-CoA content. 4. It is concluded that malonyl-CoA can be synthesized within cardiac myocytes and that the level of this metabolite can be acutely regulated. This is likely to have consequences for the regulation of carnitine palmitoyltransferase in the heart.
Awan
M M
MM
Department of Biochemistry and Molecular Biology, University College London, U.K.
Saggerson
E D
ED
D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D002470:Cell Survival; D003600:Cytosol / Q000201:enzymology; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008297:Male; D008316:Malonyl Coenzyme A / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010168:Palmitates / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000201:enzymology / Q000378:metabolism; D013566:Sympathomimetics / Q000494:pharmacology
D005227:Fatty Acids; D007773:Lactates; D010168:Palmitates; D013566:Sympathomimetics; D008316:Malonyl Coenzyme A; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases; D005947:Glucose