Identification of a Ca(2+)-dependent agarose-binding protein in chicken serum.
A Ca(2+)-dependent agarose-binding protein was purified from chicken serum by affinity chromatography on SeaKem agarose gels followed by gel filtration on Sephacryl S-300. Purified protein was eluted in a symmetrical peak at the position of molecular weight of 146,000. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it was resolved into a single protein band with a molecular weight of 39,000 under nonreducing and of 41,000 under reducing conditions. Thus, the agarose-binding protein is suggested to be composed of noncovalently-associated identical subunits which contain intrachain disulfide bonds.
Sugii
S
S
Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan.
Hirota
Y
Y
D000818:Animals; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D002645:Chickens / Q000097:blood*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000097:blood / Q000302:isolation & purification; D008970:Molecular Weight; D012016:Reference Values
D002352:Carrier Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M