Preparation of membrane vesicles from kidney cortex lysosomes using amino acid methyl ester.
A procedure for the preparation of intact membrane vesicles from kidney cortex lysosomes is reported. Highly purified preparations of lysosomes were isolated from rat and buffalo kidney cortex by Percoll density gradient centrifugation. The lysosomal membrane vesicles were prepared by osmotic rupture of the organelle with glutamate dimethyl ester. The integrity of the vesicles was demonstrated by their capacity to hold K+ and maintain H+ gradients.
Harikumar
P
P
Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay.
D000818:Animals; D002020:Buffaloes; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D005971:Glutamates; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure*; D007672:Kidney Cortex / Q000378:metabolism / Q000648:ultrastructure*; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure*; D008564:Membrane Potentials; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D011188:Potassium / Q000378:metabolism; D051381:Rats; D013050:Spectrometry, Fluorescence
D005971:Glutamates; C035873:glutamic acid dimethyl ester; D011188:Potassium