Improved method of preparation of inflated-fixed lung for radiologic-pathologic correlation.
The technique of inflation and fixation of the lung with polyethylene glycol is useful for specimen radiography and radiologic-pathologic correlation, but it is limited by poor histologic staining of the fixed tissue. To improve staining we used formalin to distend and fix the lung before standard fixation with a polyethylene glycol mixture. In this preliminary study, canine and infant lungs, and lungs from three cases of lung cancer were examined. The modified technique provided high-quality staining and satisfactory specimen radiography in all cases except one of the lung cancers; in this case excessive shrinkage occurred and degraded radiographic quality. We conclude that the new method of preparation permits both specimen radiography and high quality staining of the fixed tissue.
Satoh
K
K
Department of Radiology, Kagawa Medical School, Japan.
Sato
A
A
Nagahara
Y
Y
Kawase
Y
Y
Kobayashi
T
T
Kojima
K
K
Tanabe
M
M
D000328:Adult; D000818:Animals; D004285:Dogs; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008168:Lung / Q000000981:diagnostic imaging* / Q000473:pathology*; D008175:Lung Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology; D011092:Polyethylene Glycols; D016707:Tissue Fixation; D014057:Tomography, X-Ray Computed
D005557:Formaldehyde; D011092:Polyethylene Glycols