BST-1, a surface molecule of bone marrow stromal cell lines that facilitates pre-B-cell growth.
Bone marrow stromal cells are essential for B-lymphocyte development. However, how stromal cells regulate B lymphopoiesis is not clear. In this paper, we report the molecular cloning of a stromal cell line-derived glycosyl-phosphatidylinositol-anchored molecule, BST-1, that facilitates pre-B-cell growth. The deduced amino acid sequence of BST-1 exhibited 33% identity with CD38. BST-1 was expressed in a wide range of tissues and in umbilical vein endothelial cells, whereas it was scarcely expressed in a variety of hematopoietic cell lines. The gene for BST-1 was assigned to chromosome 14q32.3, where immunoglobulin heavy-chain genes are clustered. BST-1 expression was enhanced in rheumatoid arthritis patient-derived bone marrow stromal cell lines that were previously shown to have an enhanced ability to support the growth of a pre-B-cell line as compared with stromal cell lines derived from healthy donors.
Kaisho
T
T
Division of Molecular Oncology, Osaka University Medical School, Japan.
Ishikawa
J
J
Oritani
K
K
Inazawa
J
J
Tomizawa
H
H
Muraoka
O
O
Ochi
T
T
Hirano
T
T
D036541:ADP-ribosyl Cyclase; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D000954:Antigens, Surface / Q000235:genetics*; D001172:Arthritis, Rheumatoid / Q000503:physiopathology; D001402:B-Lymphocytes / Q000166:cytology*; D001483:Base Sequence; D001854:Bone Marrow Cells; D002883:Chromosomes, Human, Pair 14; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D006410:Hematopoiesis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2