[The effect of the amount of dialdehyde cellulose-bound enzyme on the activity of immobilized trypsin after gamma irradiation and in the storage process].
It is found the complex effect of the bound enzyme concentration on the proteolytic activity of trypsin immobilized to dialdehydecellulose (preriodate oxidation) after gamma irradiation and in process of storage. It is shown the occurrence of three stages of immobilized enzyme inactivation in process of immobilization and storage. The velocity of inactivation did not depend on bound trypsin concentration. The ratio of proteolytic activity of samples before and after gamma irradiation was increased with the increase of immobilized to carrier enzyme concentration and was not change (in range of experiment error) in process of storage. The results were compared with that of crystalline trypsin.
Belov
A A
AA
Ryl'tsev
V V
VV
Ignatiuk
T E
TE
Filatov
V N
VN
D002482:Cellulose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000528:radiation effects; D003460:Crystallization; D004337:Drug Carriers; D004356:Drug Storage; D004800:Enzymes, Immobilized / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D066298:In Vitro Techniques; D011485:Protein Binding / Q000528:radiation effects; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism / Q000528:radiation effects*
D004337:Drug Carriers; D004800:Enzymes, Immobilized; D002482:Cellulose; C058684:2,3-dialdehydocellulose; D014357:Trypsin
Vliianie kolichestva sviazannogo s dial'degidtselliulozoĭ fermenta na aktivnost' immobilizovannogo tripsina posle gamma-oblucheniia i v protsesse khraneniia.