title: Purification and characterization of a 38-kDa protein, sp38, with zona pellucida-binding property from porcine epididymal sperm.
abstract: A 38-kDa protein, sp38, was purified from the detergent extract of porcine epididymal sperm. Sp38 showed zona pellucida-binding properties similar to those of proacrosin. These two proteins specifically bound to the 90-kDa glycoprotein form of the zona pellucida components in a calcium-dependent manner. The binding of sp38 to the zona pellucida glycoprotein was inhibited by proacrosin. These findings suggest that the two proteins competitively interact with the zona pellucida during the early stage of fertilization.
authors.affiliation: Department of Immunology, University of Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Baba
authors.forename: A
authors.initials: A
authors.lastname: Iwamatsu
authors.forename: T
authors.initials: T
authors.lastname: Mori
mesh_terms: D000176:Acrosin / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004527:Egg Proteins; D004792:Enzyme Precursors / Q000378:metabolism; D004822:Epididymis / Q000737:chemistry* / Q000166:cytology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D011956:Receptors, Cell Surface; D013094:Spermatozoa / Q000737:chemistry*; D013552:Swine; D015044:Zona Pellucida / Q000378:metabolism*; D000072216:Zona Pellucida Glycoproteins
chemical_list: D002352:Carrier Proteins; D004527:Egg Proteins; D004792:Enzyme Precursors; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C022438:proacrosin; D000176:Acrosin