Automatic solid-phase extraction and high-performance liquid chromatographic determination of quinidine in plasma.
High-performance liquid chromatography (HPLC) was used for the therapeutic drug monitoring of quinidine in clinical samples. Solid-phase extraction (SPE) was studied in both off-line and on-line modes. SPE was performed in an automatic on-line mode using a fully automated Prospekt system. Extraction recoveries were in the range 97.1-99.4% for 1-2 micrograms/ml quinidine concentrations. For HPLC separation an Ultrasep RP-8 reversed-phase column was applied with acetonitrile-water (9:1) containing 0.3% triethylamine (pH 2.5) as the mobile phase. The Prospekt system is recommended for the routine monitoring of quinidine in plasma samples. Concentrations were in therapeutic range (1.2-3.6 micrograms/ml).
Brandsteterová
E
E
Department of Analytical Chemistry, Slovak Technical University, Bratislava.
Romanová
D
D
Králiková
D
D
Bozeková
L
L
Kriska
M
M
D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008991:Monitoring, Physiologic; D011802:Quinidine / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D011802:Quinidine