Adenosine affects the f-met-leu-phe induced changes in cytosolic Ca2+ and in membrane potential of human granulocytes.
Indo 1 acetoxymethyl ester and 3,3-dipentyloxacarbocyanine iodide have been used to investigate the effect of adenosine on the changes in cytosolic Ca2+ concentration and in membrane potential of human granulocytes activated by the chemotactic tripeptide f-met-leu-phe. Adenosine does not significantly affect negative (hyperpolarizing) potential changes as well as calcium accumulation in cytosol, whereas both the amplitude of the depolarization phase and the relaxation time for restoring the initial intracellular calcium level are reduced.
Capuozzo
E
E
Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
Crifò
C
C
Siems
W
W
Salerno
C
C
D000241:Adenosine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D006098:Granulocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
D009240:N-Formylmethionine Leucyl-Phenylalanine; D000241:Adenosine; D002118:Calcium