title: MAP kinase is activated during mesoderm induction in Xenopus laevis.
abstract: MAP kinase (MAPK) is activated in animal cap explants from Xenopus embryos in response to mesoderm induction by FGF. This activation is rapid, appearing within 1 min of treatment with FGF, and prolonged, lasting for at least 2 hr. By immunoblot analysis, this activation of MAPK is coupled with an electrophoretic shift to the slowly migrating, phosphorylated form of MAPK. Activin-stimulated mesoderm induction also results in the activation of MAPK, but only upon prolonged exposure. However, activin can potentiate the activation of MAPK by FGF as early as 1 min after administration. These findings suggest that MAPK is involved in the early signaling events of FGF-mediated mesoderm induction, and this involvement is modulated by other mesoderm inducers such as activin.
authors.affiliation: Howard Hughes Medical Institute, University of Colorado School of Medicine, Denver 80262.
authors.forename: R S
authors.initials: RS
authors.lastname: Hartley
authors.forename: A L
authors.initials: AL
authors.lastname: Lewellyn
authors.forename: J L
authors.initials: JL
authors.lastname: Maller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004627:Embryonic Induction; D004789:Enzyme Activation; D005306:Fertilization; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D019950:Mitogen-Activated Protein Kinase 1; D008969:Molecular Sequence Data; D010063:Ovum / Q000201:enzymology; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D010455:Peptides; D016222:Fibroblast Growth Factor 2; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D019950:Mitogen-Activated Protein Kinase 1