Corticotropin-releasing factor is secreted in the BE(2)-C neuroblastoma cell and is responsive to forskolin.
The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 microM retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.
Kasckow
J W
JW
Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Atlanta, GA 30322.
Owens
M J
MJ
Stipetic
M D
MD
Han
J H
JH
Nemeroff
C B
CB
D005576:Colforsin / Q000494:pharmacology*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D005576:Colforsin; D003346:Corticotropin-Releasing Hormone