Effects of activated eosinophils cultured from human umbilical cord blood on guinea pig trachealis.
We studied the biochemical indexes and corresponding induction of airway smooth muscle contraction and hyperresponsiveness in guinea pig trachealis in situ caused by cultured eosinophils derived from mononuclear cell fractions of human umbilical cord blood. A method was developed that permitted isolation of large numbers of cells (approximately 2.6 x 10(6)/ml cord blood) having morphological and immunohistological characteristics of human peripheral blood eosinophils. After activation with 10(-6) M formyl-Met-Leu-Phe + 5 micrograms/ml cytochalasin B (fMLP + B), in situ application to the epithelial surface of 6 x 10(6) cord-derived eosinophils (CDE)/surface area (cm2) caused 1.46 +/- 0.24 g/cm maximal active tracheal tension in guinea pig tracheal smooth muscle (P < 0.005 vs. zero baseline). Muscarinic responsiveness also was augmented in situ in trachealis preparations treated with activated 3-wk CDE. Contraction caused by 3 x 10(-7) mol/kg iv methacholine (MCh) was 0.94 +/- 0.18 g/cm at baseline vs. 1.80 +/- 0.24 g/cm after activated CDE (P = 0.02). Control (sham-activated) 3-wk CDE caused neither significant contraction [0.41 +/- 0.16 g/cm active tension (AT); P < 0.05 vs. fMLP+B] nor augmented muscarinic responsiveness. Cells cultured for 5 wk contained fewer granules than 3-wk CDE and also caused less direct contraction of trachealis (0.73 +/- 0.14 g/cm AT) after activation (P < 0.01 vs. 3-wk CDE). Both contraction and muscarinic augmentation were blocked in 3-wk CDE after blockade of leukotriene C4 (LTC4) synthesis by pretreatment with the 5-lipoxygenase inhibitor, A63162 (50 microM). Treatment with A63162 had no effect on the stimulated release of eosinophil peroxidase.(ABSTRACT TRUNCATED AT 250 WORDS)
Hamann
K J
KJ
Department of Medicine, University of Chicago, Illinois 60637.
Strek
M E
ME
Baranowski
S L
SL
Munoz
N M
NM
Williams
F S
FS
White
S R
SR
Vita
A
A
Leff
A R
AR
D000818:Animals; D001773:Blood Cells / Q000502:physiology*; D002478:Cells, Cultured; D004804:Eosinophils / Q000201:enzymology / Q000502:physiology*; D005312:Fetal Blood; D006168:Guinea Pigs; D006801:Humans; D017997:Leukotriene C4 / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D009195:Peroxidase / Q000378:metabolism; D014132:Trachea / Q000187:drug effects / Q000502:physiology*
D016859:Lipoxygenase Inhibitors; D016210:Methacholine Chloride; D017997:Leukotriene C4; D009195:Peroxidase