title: Glycoprotein patterns in Borrelia spp.
abstract: The presence of glycoproteins in several Borrelia species was investigated by the digoxigenin labelling technique. The outer surface proteins A and B of seven isolates of the Lyme disease spirochete B. burgdorferi showed to be major glycosylated proteins. Few minor polypeptides with variable molecular masses were also present, at variance, in B. burgdorferi strains. Minor glycosylated proteins with varying molecular masses have been detected in the relapsing fever borreliae B. hermsii, B. turicatae and B. parkeri. B. turicatae showed also a major glycosylated protein with a molecular mass of approximately 40 kDa. Animal pathogenic borreliae B. anserina and B. coriaceae presented only minor glycosylated proteins with variable molecular masses.
authors.affiliation: Institute of Microbiology, University of Bologna, S. Orsola Hospital, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Sambri
authors.forename: F
authors.initials: F
authors.lastname: Massaria
authors.forename: M
authors.initials: M
authors.lastname: Ardizzoni
authors.forename: C
authors.initials: C
authors.lastname: Stefanelli
authors.forename: R
authors.initials: R
authors.lastname: Cevenini
mesh_terms: D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000737:chemistry; D001428:Bacterial Vaccines; D015153:Blotting, Western; D001898:Borrelia / Q000737:chemistry*; D015748:Borrelia burgdorferi Group / Q000737:chemistry; D004076:Digoxigenin; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D008074:Lipoproteins
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D006023:Glycoproteins; D008074:Lipoproteins; C072045:OspA protein; C077030:OspB protein, Borrelia burgdorferi; D004076:Digoxigenin