Biopolymer synthesis on polypropylene supports. I. Oligonucleotides.
We have modified polypropylene to serve as a new solid-phase support for oligonucleotide synthesis. The plastic is first surface aminated by exposure to an ammonia plasma generated by radiofrequency plasma discharge. The aminated polypropylene has been found to be useful as a support for the in situ synthesis of oligonucleotides from monomers. Furthermore, oligonucleotides synthesized on the surface of the plastic remain attached following deprotection and can be used directly for hybridization.
Matson
R S
RS
Advanced Development Unit, Beckman Instruments, Fullerton, California 92634.
Rampal
J B
JB
Coassin
P J
PJ
D001483:Base Sequence; D003550:Cystic Fibrosis / Q000235:genetics; D004247:DNA / Q000138:chemical synthesis; D018076:DNA, Complementary / Q000138:chemical synthesis; D005091:Exons; D006801:Humans; D007455:Iodine; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000235:genetics; D009943:Organophosphorus Compounds; D010084:Oxidation-Reduction; D011126:Polypropylenes
D018076:DNA, Complementary; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D011126:Polypropylenes; D004247:DNA; D007455:Iodine