title: Clinical correlates of circulating immune complexes and antibody reactivity in squamous cell carcinoma of the head and neck. The Department of Veterans Affairs Laryngeal Cancer Study Group.
abstract: OBJECTIVE
  To evaluate the correlation between the presence and titer of host-derived antibody reactivity, circulating immune complexes, and clinical course and prognosis in patients with squamous cell carcinoma of the head and neck (SCCHN).
  
  
  METHODS
  Serum samples, obtained from untreated patients with squamous cell carcinoma of the larynx entered onto a multiinstitutional trial, were evaluated for the presence of elevated circulating immune complexes (221 patients) and host-derived antibody directed against two SCCHN cell lines (107 patients).
  
  
  RESULTS
  Patients had significantly elevated levels of circulating immune complexes as measured by C1q binding compared with normal controls. Patients with higher levels of circulating immune complexes were less likely to respond to chemotherapy. No correlations were noted between immune complex levels and stage of disease, nodal status, site of disease, recurrence, or survival. Evaluation of native antibody titers for their relationship to clinical correlates showed no statistically significant associations. In sera subjected to immune complex dissociation, patients with moderately or poorly differentiated tumors had significantly higher antibody titers when compared with patients with well-differentiated tumors. Because marked variation in the increase of antibody titers following immune complex dissociation was noted, the ratio of immune complex-dissociated to native antibody titer was examined. Patients with a high ratio had a lower proportion of complete and partial responses to chemotherapy.
  
  
  CONCLUSIONS
  Our results support the conclusion that the formation of tumor-associated immune complexes in patients with SCCHN is associated with a decreased response to chemotherapy.
authors.affiliation: Department of Medicine, Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, PA.
authors.forename: D R
authors.initials: DR
authors.lastname: Vlock
authors.forename: S P
authors.initials: SP
authors.lastname: Schantz
authors.forename: S G
authors.initials: SG
authors.lastname: Fisher
authors.forename: H E
authors.initials: HE
authors.lastname: Savage
authors.forename: T E
authors.initials: TE
authors.lastname: Carey
authors.forename: G T
authors.initials: GT
authors.lastname: Wolf
mesh_terms: D000912:Antibodies, Neoplasm / Q000097:blood*; D000936:Antigen-Antibody Complex / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000276:immunology*; D011379:Prognosis; D011446:Prospective Studies; D014407:Tumor Cells, Cultured
chemical_list: D000912:Antibodies, Neoplasm; D000936:Antigen-Antibody Complex