title: [Ligase chain reaction (LCR)].
abstract: Ligase chain reaction (LCR) is a ligation-mediated amplification technique of a target DNA sequence using oligonucleotides and thermostable ligase. LCR is useful for the detection of known DNA sequences and point mutations in a limited amount of DNA. We introduce the principle, development, and protocol of this simple and convenient technique for DNA analysis.
authors.affiliation: Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamanishi
authors.forename: H
authors.initials: H
authors.lastname: Yasuno
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D011088:DNA Ligases; D005914:Globins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000235:genetics; D017354:Point Mutation
chemical_list: D009841:Oligonucleotides; D005914:Globins; D004247:DNA; D011088:DNA Ligases