Smaller number of large myelinated fibers and focal myelin thickening in mutant quails deficient in neurofilaments.
The peripheral nervous system of a mutant of a Japanese quail deficient in neurofilaments (mutant) and of a normal Japanese quail (control) was morphometrically evaluated to characterize the morphological findings, especially those of the myelinated fibers of the mutant. In the proximal peroneal nerves, the frequency of the teased myelinated fibers showing the focal myelin thickening was higher in mutant than in control (P < 0.001) without obvious ongoing axonal degeneration and segmental demyelination. The total numbers of the myelinated fibers in the proximal and distal peroneal nerve, and in the tibial nerve branch to gastrocnemius muscle (pars medialis) were similar between control and mutant, although the number of the large myelinated fibers was less (P < 0.01) and the number of the small myelinated fibers was greater (P < 0.01) in mutant compared with control. The median diameters of neuronal cell bodies of the sacral dorsal root ganglia were similar in control and mutant. The percentages of light, dark and unclassified cells evaluated based on the histologic cytoplasmic features were also similar in control and mutant. Therefore, morphometric alterations were more pronounced in the peripheral myelinated nerve fibers compared with those in the cell bodies of the spinal dorsal root ganglia. We concluded that a smaller number of large myelinated fibers with a greater number of small myelinated fibers and the presence of focal myelin thickening are the main morphologic findings in this mutant, probably due to the arrest of radial growth or maturation of the axons of the myelinated fibers in the absence of ongoing myelinated fiber degeneration.
Zhao
J X
JX
Department of Neurology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Ohnishi
A
A
Itakura
C
C
Mizutani
M
M
Yamamoto
T
T
Hayashi
H
H
Murai
Y
Y
D000818:Animals; D003370:Coturnix; D005260:Female; D005727:Ganglia, Spinal / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009132:Muscles / Q000294:innervation / Q000473:pathology; D009154:Mutation; D009186:Myelin Sheath / Q000648:ultrastructure*; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure*; D009474:Neurons / Q000502:physiology*; D009802:Oculomotor Nerve / Q000473:pathology; D010523:Peripheral Nervous System Diseases / Q000235:genetics* / Q000473:pathology; D010543:Peroneal Nerve / Q000473:pathology