Evaluation of radioactive and nonradioactive gene probes and cell culture for detection of poliovirus in water samples.
Five nonradioactive probe assays were evaluated by using chemiluminescent and colormetric signals, along with two isotopic assays and cell culture, for the detection of poliovirus in concentrated water samples. In environmental samples, a 100% correlation existed between digoxigenin and single-stranded [32P]RNA probes. All probe assays detected more positive samples than the cell culture did.
Moore
N J
NJ
Department of Microbiology, University of New Hampshire, Durham 03824.
Margolin
A B
AB
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D015336:Molecular Probe Techniques / Q000706:statistics & numerical data; D010761:Phosphorus Radioisotopes; D017955:Poliovirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods; D014871:Water Microbiology
D010761:Phosphorus Radioisotopes