Introduction of functional groups onto the surface of polyethylene for protein immobilization.
Amino and carboxyl groups could be introduced onto the surface of high-density polyethylene film by utilizing graft polymerization of acrylamide and the subsequent Hofmann degradation and alkaline hydrolysis of grafted polyacrylamide. Graft polymerization was carried out by immersing an argon-plasma treated film in an aqueous solution of the monomer, followed by heating after degassing the monomer/film mixture. The surface density of these functional groups could be increased up to 10(-7) mol/cm2. The surfaces having amino and carboxyl groups exhibited positive and negative zeta potentials, respectively, when contacted with KCl aqueous solution. Both of the functional groups introduced onto the polyethylene surface were found to be utilizable for covalent immobilization of protein using carbodiimide for the carboxylic group or mediators such as glutaraldehyde and ethylene glycol diglycidyl ether for the amino group.
Sano
S
S
Research Center for Biomedical Engineering, Kyoto University, Japan.
Kato
K
K
Ikada
Y
Y
D000180:Acrylic Resins / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D002234:Carbodiimides / Q000737:chemistry; D005026:Ethylene Glycols / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011095:Polyethylenes / Q000737:chemistry*; D011506:Proteins; D013205:Staphylococcal Protein A; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002234:Carbodiimides; D005026:Ethylene Glycols; D005976:Glutaral; D011095:Polyethylenes; D011506:Proteins; D013205:Staphylococcal Protein A; D000099166:Polyacrylamides