L3p40-50--a new lung-tumor-associated 40- to 50-kDa antigen defined by a monoclonal antibody (L3).
A monoclonal antibody (MAb), designated L3, was raised against a human-lung-adenocarcinoma-derived cell line, A549. Immunohistological studies showed reactivity of MAb L3 with 70 to 90% of lung tumors representing the 4 major histotypes. Strongest reactivity was seen with squamous-cell cancer and adenocarcinoma, with strong intracellular staining of the tumor cells. In small-cell lung cancer, staining was seen in a minority of tumor cells. Normal tissue staining was seen with thyroid follicular cells and focally with epithelial cells of the bronchi, the colon and the small intestine. Normal liver parenchyma was negative, whereas hepatocytes surrounding metastatic lesions were strongly stained. This pattern of staining was also seen for connective tissue. Western-blot analysis showed that the antigen defined by MAb L3 has an apparent molecular weight of 40 to 50 kDa, and hence is denominated L3p40-50. Our results suggest that L3p40-50 represents an undescribed tumor antigen.
Brezicka
F T
FT
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Olling
S
S
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000737:chemistry / Q000166:cytology*; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology* / Q000601:surgery; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight; D012016:Reference Values; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C087368:L3p40-50 antigen