title: Combinatorial interactions between positive and negative cis-acting elements control spatial patterns of 4CL-1 expression in transgenic tobacco.
abstract: The phenylpropanoid enzyme 4-coumarate:coenzyme A ligase (4CL) plays a key role in linking general phenylpropanoid metabolism to end-product specific biosynthetic pathways. During vascular system and floral organ differentiation, the parsley 4CL-1 gene is expressed in a restricted set of tissues and cell types where 4CL activity is required to supply precursors for the synthesis of diverse phenylpropanoid-derived products such as lignin and flavonoids. In order to localize cis-acting elements which specify complex patterns of 4CL-1 expression, we analyzed the expression of internally deleted promoter fragment-GUS fusions in tobacco plants and parsley protoplasts. Elements located between -244 and -78 were required for most aspects of developmentally regulated expression. Within this region, three separate promotor domains containing partially redundant cis-elements directed vascular-specific expression when combined with a TATA-proximal domain. A negative cis-acting element which represses phloem expression was revealed in one of the domains and appears to be responsible for restricting vascular expression to the xylem. Distinct but overlapping promoter domain combinations were required for expression in floral organs, suggesting that different combinations of cis-acting elements may direct expression in different organs. Gel retardation assays were used to demonstrate the formation of DNA-protein complexes between factors present in nuclear extracts of parsley tissue culture cells and various tobacco organs and a 4CL-1 promoter fragment. Competition experiments showed that complex formation required the presence of a 42 bp promoter domain shown to be critical for 4CL-1 expression in vascular and floral tissues. The results are discussed in light of the coordinate expression of 4CL and other phenylpropanoid genes.
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, Canada.
authors.forename: K D
authors.initials: KD
authors.lastname: Hauffe
authors.forename: S P
authors.initials: SP
authors.lastname: Lee
authors.forename: R
authors.initials: R
authors.lastname: Subramaniam
authors.forename: C J
authors.initials: CJ
authors.lastname: Douglas
mesh_terms: D001483:Base Sequence; D003066:Coenzyme A Ligases / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D014018:Tissue Distribution; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
chemical_list: D004247:DNA; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase