Determination of tylosin and tilmicosin residues in animal tissues by reversed-phase liquid chromatography.
A method for the simultaneous determination of tylosin and tilmicosin residues in animal tissues is reported. Solid-phase extraction columns are used to isolate the drugs from tissue extracts. Determination is accomplished by reversed-phase liquid chromatography with UV detection at 287 nm. Mean recoveries from spiked tissues were 79.9% (coefficient of variation [CV], 8.1%) for tylosin and 92.6% (CV, 8.7%) for tilmicosin. Detection limits for tylosin and tilmicosin were 0.020 and 0.010 ppm, respectively.
Chan
W
W
Agriculture Canada, Health of Animals Laboratory, Saskatoon, SK.
Gerhardt
G C
GC
Salisbury
C D
CD
D000818:Animals; D000900:Anti-Bacterial Agents; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D007668:Kidney / Q000737:chemistry*; D018942:Macrolides; D009132:Muscles / Q000737:chemistry*; D012015:Reference Standards; D013552:Swine; D015645:Tylosin / Q000031:analogs & derivatives* / Q000032:analysis*
D000900:Anti-Bacterial Agents; D018942:Macrolides; C052319:tilmicosin; D015645:Tylosin