4-Dimethylaminophenethylamine, a sensitive, specific, electrochemically detectable monoamine oxidase-B substrate.
4-Dimethylaminophenethylamine (DMAPEA) was characterized as an MAO substrate. This compound was unaffected by MAO-A, while its oxidation by MAO-B was linear as a function of both time and enzyme concentration, with Km = 5.8 microM and Vmax = 21.2 pmol/min/mg protein, using a crude rat brain mitochondrial suspension as source of MAO. Both DMAPEA and its oxidation product, 4-dimethylaminophenylacetic acid (DMAPAA), can be detected electrochemically at 0.85 V. The high MAO-B affinity and selectivity of DMAPEA, together with its low oxidation potential, make this molecule a unique tool to determine MAO-B activity in a wide variety of tissue preparations using HPLC-ED.
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Cell Biology Division, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Scorza
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Dajas
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D000818:Animals; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D003010:Clorgyline / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010627:Phenethylamines / Q000737:chemistry / Q000378:metabolism*; D010648:Phenylacetates / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D012642:Selegiline / Q000494:pharmacology
D010627:Phenethylamines; D010648:Phenylacetates; C030748:4-N,N-dimethylaminophenylacetic acid; D012642:Selegiline; C087509:4-dimethylaminophenethylamine; D008995:Monoamine Oxidase; D003010:Clorgyline