title: Meristem-specific gene expression directed by the promoter of the S-phase-specific gene, cyc07, in transgenic Arabidopsis.
abstract: A genomic clone for the cyc07 gene, which is expressed specifically at the S phase during the cell cycle in synchronous cultures of periwinkle (Catharanthus roseus) cells, was isolated. Determination of the nucleotide sequence of the clone revealed that the cyc07 gene consists of seven exons separated by six introns. Genomic Southern analysis indicated that the cyc07 gene is present as a single copy per haploid genome in periwinkle. Expression of related genes was detected in a wide range of other plants. Transgenic Arabidopsis plants were generated that expressed the gene for beta-glucuronidase (GUS) under the control of the promoter of the cyc07 gene. The tissue-specific pattern of expression directed by the promoter was investigated by analysis of GUS activity. Histochemical tests demonstrated that 589 bp of the 5'-upstream sequence of the cyc07 gene could direct specifical expression of the GUS reporter gene in meristematic tissues in transgenic plants. The spatial pattern of expression directed by the promoter was closely correlated with meristematic activity and cell proliferation, suggesting an association between the function of the cyc07 gene and cell proliferation.
authors.affiliation: Nagoya University BioScience Center, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Fukuda
authors.forename: A
authors.initials: A
authors.lastname: Komamine
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D018797:Cell Cycle Proteins; D005786:Gene Expression Regulation / Q000502:physiology; D017343:Genes, Plant; D008969:Molecular Sequence Data; D059828:Plant Cells; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D010944:Plants / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016196:S Phase / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
chemical_list: D018797:Cell Cycle Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C081079:cyc07 protein, Catharanthus roseus