title: Development of an enzyme-linked immunosorbent assay for isoproturon in water.
abstract: A competitive enzyme-linked immunosorbent assay (ELISA) suitable for the determination of the urea herbicide isoproturon [3-(4-isopropylphenyl)-1,1-dimethyl urea] in water has been developed. A derivative of isoproturon [3-(4-isopropylphenyl)-1-carboxypropyl-1-methyl urea] has been synthesized and linked to thyroglobulin using the N-hydrosuccinimide reaction. The immunogen was used to immunize two sheep, which both responded by producing specific antibodies to isoproturon with little cross-reactivity to various structurally related and unrelated pesticides. The enzyme label was prepared by coupling the hapten to horseradish peroxidase using the above reaction. The coated-antibody competitive ELISA, which has a sensitivity of 0.03 microgram l-1, permits the direct determination of isoproturon in various water matrices and can facilitate the monitoring of water quality in drinking-water supplies.
authors.affiliation: Robens Institute, University of Surrey, Guildford, UK.
authors.forename: M F
authors.initials: MF
authors.lastname: Katmeh
authors.forename: G
authors.initials: G
authors.lastname: Frost
authors.forename: W
authors.initials: W
authors.lastname: Aherne
authors.forename: D
authors.initials: D
authors.lastname: Stevenson
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006540:Herbicides / Q000032:analysis* / Q000276:immunology; D008782:Methylurea Compounds / Q000032:analysis* / Q000276:immunology; D010671:Phenylurea Compounds; D012756:Sheep / Q000276:immunology; D014881:Water Supply / Q000032:analysis*
chemical_list: D006540:Herbicides; D008782:Methylurea Compounds; D010671:Phenylurea Compounds; C028904:isoproturon