(Aminomethyl)phosphonate derivatives of oligonucleotides.
Oligothymidylate (aminomethyl)phosphonates have been prepared, and their enzymatic and physicochemical properties have been studied. The individual isomers of the protected dimers have been separated, characterized, and incorporated into oligonucleotides in which the backbone consists of alternating (aminomethyl)phosphonate and phosphodiester linkages. One of these net neutral, single isomer oligonucleotides forms a duplex with its complementary sequence which is more stable than the corresponding natural counterpart, whereas the other isomer is considerably less stable. Specificity of hybridization is maintained, as determined by the reduction in melting temperature observed upon the introduction of mismatches into the complementary strand of the duplex. The (aminomethyl)-phosphonate linkage is stable toward enzymatic degradation but can be hydrolyzed in aqueous solution at elevated temperature.
Fathi
R
R
PharmaGenics, Inc., Allendale, New Jersey 07401.
Huang
Q
Q
Syi
J L
JL
Delaney
W
W
Cook
A F
AF
D001483:Base Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D012680:Sensitivity and Specificity; D012996:Solutions; D013329:Structure-Activity Relationship
D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D012996:Solutions; C036423:1-aminomethylphosphonic acid; D010758:Phosphorus; D010100:Oxygen