In vitro inactivation of glucose-6-phosphate dehydrogenase from human red blood cells by acrolein: a possible biomarker of exposure.
We have investigated the possibility of utilizing glucose-6-phosphate dehydrogenase (G6PD) as a macromolecular (biological) marker of acrolein exposure. The result showed a dose-dependent inactivation of the erythrocyte G6PD in situ or as a purified enzyme from human erythrocytes or yeast. Amino acid analysis on the chemically modified yeast G6PD showed a formation of a lysine adduct which is probably linked to the inactivation.
Trieff
N M
NM
Department of Preventive Medicine, University of Texas Medical Branch, Galveston 77555-1010.
Ficklen
D
D
Gan
J
J
D000171:Acrolein / Q000494:pharmacology / Q000633:toxicity; D000596:Amino Acids / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure / Q000032:analysis*; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000037:antagonists & inhibitors*; D006801:Humans; D066298:In Vitro Techniques
D000596:Amino Acids; D015415:Biomarkers; D000171:Acrolein; D005954:Glucosephosphate Dehydrogenase