Image and flow cytometry: companion techniques for adherent and non-adherent cell analysis and sorting.
Flow cytometry (FMC) is an analytical and preparative technique whereas image analysis is only applied to cell analysis. Recently, image analysis has been adapted as a preparative method using a new technique: image cytometry for analysis and sorting (ICAS). FCM and ICAS are complementary. Flow cytometry allows rapid, quantitative and precise study of fluorescence and light scattering in a large number of cells in suspension, while ICAS analyses fewer cells (adherent cells or tissue) on the basis of fluorescence, morphology and size. ICAS can use these criteria to destroy unwanted cells and hence sort selected cells. ICAS can also be used for confocal microscopy and laser surgery.
Métézeau
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Service INSERM-Pasteur de Cytométrie analytique et préparative, CNRS, URA 1129, Paris, France.
D002448:Cell Adhesion; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D013048:Specimen Handling; D013535:Suspensions
D013535:Suspensions