Exon structure at the human ACP1 locus supports alternative splicing model for f and s isozyme generation.
The human ACP1 locus encodes a genetically polymorphic cytoplasmic low-molecular-weight acid phosphatase. Each of the common alleles encodes two isoforms, f and s. Both isozymes are of equal length (157 residues) but differ in sequence over an internal 34 residue segment. Substantial portions of the ACP1*A, *B and *C alleles common to Europeans have been sequenced. Six linearly positioned exons containing codons 14 to 157 were identified. Two exons of equal length (114bp) interspaced by a short (41bp), probably nonfunctional, intron encode the specific f and s segments, respectively. These findings strongly support an alternative RNA splicing hypothesis. In addition, three allele-specific base substitutions were encountered.
Lazaruk
K D
KD
Forensic Science Group, School of Public Health, University of California, Berkeley.
Dissing
J
J
Sensabaugh
G F
GF
D000135:Acid Phosphatase / Q000235:genetics*; D000483:Alleles; D017398:Alternative Splicing; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D007438:Introns / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D007527:Isoenzymes; D000135:Acid Phosphatase