title: Effect of protein adsorption on the blood-contacting response of sulphonated polyurethanes.
abstract: Polyurethanes which are grafted with propyl sulphonate functionality have excellent blood-contacting properties. In a canine ex vivo series shunt experiment, very low platelet deposition was observed on these materials and those platelets which were adherent remained unspread. In contrast to this, large amounts of fibrinogen, of the order of a monolayer or greater, were deposited on these surfaces in this ex vivo experiment. This led to the hypothesis that perhaps the deposited fibrinogen did not retain its platelet-adhesive activity. In this paper, we investigate the possibility that these materials exert their antithrombotic effects through the adsorbed protein layer. Protein adsorption kinetics and isotherms on these sulphonated polyurethanes are determined. Multilayer protein adsorption or absorption into the hydrogel-like materials is found for each of the proteins studied, and the greatest amounts of protein are seen on the most highly sulphonated polyurethanes. Further, the blood-contacting response of these materials is investigated after pre-conditioning with either fibrinogen, fibronectin or albumin. When these materials are pre-adsorbed with either fibrinogen or fibronectin, a very thrombogenic response was observed, which suggests that the platelet-adhesive activity of these proteins is not being reduced. Pre-adsorption of albumin did not improve the thromboresistance of these surfaces.
authors.affiliation: Department of Chemical Engineering, University of Wisconsin, Madison 53706.
authors.forename: J H
authors.initials: JH
authors.lastname: Silver
authors.forename: H B
authors.initials: HB
authors.lastname: Lin
authors.forename: S L
authors.initials: SL
authors.lastname: Cooper
mesh_terms: D000327:Adsorption; D017738:Alkanesulfonic Acids; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000502:physiology*; D001798:Blood Proteins / Q000378:metabolism*; D004285:Dogs; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010974:Platelet Aggregation; D011140:Polyurethanes / Q000737:chemistry*; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D017738:Alkanesulfonic Acids; D001672:Biocompatible Materials; D001798:Blood Proteins; D005353:Fibronectins; D011140:Polyurethanes; D012709:Serum Albumin; C068559:propylsulfonic acid; D005340:Fibrinogen