title: Cloning genes from an inner ear cDNA library.
abstract: A rat inner ear complementary DNA (cDNA) library containing 1.9 x 10(6) recombinants was constructed and evaluated. Inserts averaged 2.0 (+/- 2.1) kilobases in length. A subset of inserts was screened for site of expression. Two cDNA transcripts were isolated based on cochlear expression restricted to the spiral ganglion. One transcript showed a high degree of homology to several long interspersed DNA elements, neuron-specific nuclear transcripts thought to be involved in gene regulation. The second transcript showed no homology to known sequences and appears to encode a neuron-specific protein of about 248 amino acids. The library can be used to identify proteins important for inner ear function and disease.
authors.affiliation: Department of Surgery, University of California-San Diego School of Medicine, La Jolla.
authors.forename: A F
authors.initials: AF
authors.lastname: Ryan
authors.forename: S
authors.initials: S
authors.lastname: Batcher
authors.forename: D
authors.initials: D
authors.lastname: Brumm
authors.forename: K
authors.initials: K
authors.lastname: O'Driscoll
authors.forename: J P
authors.initials: JP
authors.lastname: Harris
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003051:Cochlea; D018076:DNA, Complementary / Q000235:genetics*; D007758:Ear, Inner; D005784:Gene Amplification; D015723:Gene Library; D051381:Rats
chemical_list: D018076:DNA, Complementary