Preformed purified peptide/major histocompatibility class I complexes are potent stimulators of class I-restricted T cell hybridomas.
A panel of antigen-specific, major histocompatibility complex class I-restricted T cell hybridomas has been generated to examine the capacity of peptide/class I complexes to stimulate T cells at the molecular level. Peptide/class I complexes were generated in detergent solution, purified and quantitated. Latex particles were subsequently coated with known amounts of preformed complexes and used to stimulate the T cell hybridomas. Stimulation was specific, i.e. only the appropriate peptide/class I combination were stimulatory, and quite sensitive, i.e. as little as 300 complexes per bead could be detected by the T cells. Preformed complexes were about 500,000 times more potent than free peptide in terms of T cell stimulation, demonstrating the physiological relevancy of the biochemically generated complexes. Surprisingly, the majority (including the most sensitive of the hybridomas) had lost CD8 expression, suggesting that antigen-specific stimulation of class I-restricted T cell hybridomas, as assessed by IL-2 release, does not depend on CD8.
Stryhn
A
A
Institute of Medical Microbiology and Immunology, University of Copenhangen, Denmark.
Pedersen
L O
LO
Ortiz-Navarrete
V
V
Buus
S
S
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D016827:CD8 Antigens / Q000096:biosynthesis; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology; D007840:Latex / Q000737:chemistry; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D016827:CD8 Antigens; D015395:Histocompatibility Antigens Class I; D007840:Latex; D010455:Peptides