Detection of the L protein of tomato spotted wilt virus.
The 5'-terminal and 3'-terminal parts of the single open reading frame (ORF) in the L RNA of tomato spotted wilt virus (TSWV) were expressed using a prokaryotic expression system. Using antibodies raised against the translational products obtained a 330-kDa protein could be specifically detected in preparations of purified virions and in nucleocapsid preparations from TSWV-infected leaf tissue. The results obtained indicate that the L protein of TSWV, though much larger than that of the animal-infecting bunyaviruses, is present in virus particles in an unprocessed, intact form.
van Poelwijk
F
F
Boye
K
K
Oosterling
R
R
Peters
D
D
Goldbach
R
R
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism*; D017865:Tospovirus / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D017931:DNA Primers; D012367:RNA, Viral; D014764:Viral Proteins