Improvement of the merthiolate-iodine-formalin (MIF) fecal technique for hookworm, Trichuris trichiura, and Ascaris lumbricoides eggs.
The merthiolate-iodine-formalin (MIF) technique to detect human intestinal parasites in feces was inefficient for the detection of hookworm and Trichuris trichiura eggs. Addition of a saturated-brine flotation step increased recovery of hookworm eggs by 77% and Trichuris trichiura eggs by 87%. Recovery of Ascaris lumbricoides also was increased by 71%. This was a significant improvement in the sensitivity of the MIF technique for these 3 kinds of human parasite.
Bawden
M P
MP
Overseas Business Success, Lewistown, Montana 59457.
D000723:Ancylostomatoidea / Q000302:isolation & purification*; D000818:Animals; D017164:Ascaris lumbricoides / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005557:Formaldehyde; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis*; D007455:Iodine; D010270:Parasite Egg Count / Q000379:methods; D012680:Sensitivity and Specificity; D013849:Thimerosal; D014258:Trichuris / Q000302:isolation & purification*
D005557:Formaldehyde; D013849:Thimerosal; D007455:Iodine