Structure and cell cycle-regulated transcription of the human cyclin A gene.
Cyclin A is a cell cycle regulatory protein that functions in mitotic and S-phase control in mammalian somatic cells. Its deregulated expression may have a role in cellular transformation. We have cloned and sequenced the human cyclin A gene and cDNAs representing its mRNAs and have characterized its promoter. Using synchronized cultures of NIH 3T3 cells stably transfected with cyclin A promoter/luciferase constructs, we show that the promoter is repressed during the G1 phase of the cell cycle and is activated at S-phase entry. Cell cycle regulation of the cyclin A gene promoter is mediated by sequences extending from -79 to +100 relative to the predominant transcription start site. It does not require the presence of a functional retinoblastoma protein.
Henglein
B
B
Institut National de la Santé et de la Recherche Médicale, Unité 370, Institut Necker, Paris, France.
Chenivesse
X
X
Wang
J
J
Eick
D
D
Bréchot
C
C
D001483:Base Sequence; D002453:Cell Cycle; D003001:Cloning, Molecular; D016213:Cyclins / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D016213:Cyclins; D012333:RNA, Messenger