title: Comparison of antibody concentrations and protective activity of respiratory syncytial virus immune globulin and conventional immune globulin.
abstract: Relative to conventional immune globulins (IG, 13 lots), IGs prepared from donors with high activity by microneutralization assay to respiratory syncytial virus (RSVIG, 8 lots) had significantly higher neutralizing antibodies to 6 RSV strains (mean enrichment, 5.2-fold; range, 2.6- to 10.0-fold). In contrast, IgG antibody concentrations to whole RSV, fusion protein, or glycoproteins of A and B strains were similar in RSVIG and IG. Treatment of cotton rats with RSVIG at 0.5 g/kg 24 h before RSV challenge reduced RSV by 99% in the lungs (P < .001). RSVIG at 5.0 g/kg reduced RSV by 99% in the nose. IG at 5.0 g/kg had efficacy similar to that of RSVIG at 0.5 g/kg. Serum plaque-reduction neutralization titers of 1/390 resulted in 99% reduction of lung RSV and titers of 1/3500 resulted in 99% reduction in nose RSV. Relative to IG, RSVIG is enriched selectively in RSV neutralizing antibodies and has approximately 10 times greater protective activity in cotton rats.
authors.affiliation: Massachusetts Public Health Biologic Laboratories, Jamaica Plain 02130-3597.
authors.forename: G R
authors.initials: GR
authors.lastname: Siber
authors.forename: D
authors.initials: D
authors.lastname: Leombruno
authors.forename: J
authors.initials: J
authors.lastname: Leszczynski
authors.forename: J
authors.initials: J
authors.lastname: McIver
authors.forename: D
authors.initials: D
authors.lastname: Bodkin
authors.forename: R
authors.initials: R
authors.lastname: Gonin
authors.forename: C M
authors.initials: CM
authors.lastname: Thompson
authors.forename: E E
authors.initials: EE
authors.lastname: Walsh
authors.forename: P A
authors.initials: PA
authors.lastname: Piedra
authors.forename: V G
authors.initials: VG
authors.lastname: Hemming
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D009500:Neutralization Tests; D051381:Rats; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000517:prevention & control; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D016205:Sigmodontinae
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G