[Study on endocytosis and nuclear accumulation of epidermal growth factor (EGF) in normal C3H mouse fibroblast cells C3H10 T1/2C18 and 3H-TdR transformed counterpart cells].
In this study, we investigated endocytosis and nuclear accumulation of epidermal growth factor (EGF) in normal C3H mouse embryo fibroblast cells C3H10 T1/2 C18 (designated NC3H10) and 3H-TdR transformed counterpart cells (designated TC3H10). The results revealed that 125I-labelled EGF bound to the receptor on the cell surface of the two cell lines was subsequently endocytosed and translocated from the cell membrane to the nucleus, where it progressively accumulated. NC3H10 showed more EGF accumulation in nucleus than TC3H10 (p < 0.05). SDS-polyacrylamide gel electrophoresis revealed that the endocytosed 125I-EGF associated with the nucleus was intact, with negligible breakdown products present. The nuclear accumulation of intact EGF in the mentioned cell lines exposed to ammonium chloride (a lysosomal inhibitor) was higher than the control (p < 0.05). These results suggested that receptor-mediated endocytosis and nuclear accumulation of EGF may play an important role in mediation some of the action of EGF.
Peng
Y
Y
Department of Biochemistry, Beijing Medical University.
Tong
T J
TJ
D000818:Animals; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D004705:Endocytosis; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D008809:Mice, Inbred C3H
D004815:Epidermal Growth Factor; D066246:ErbB Receptors