[Vesiculation of erythrocytes during storage and connection of it with other processes in the cell].
The activation energies of three processes (AT-depended vesiculation, release of potassium ions from erythrocytes and formation of methemoglobin) in erythrocytes incubated in phosphate buffer are measured in the range 4-37 degrees C. It is show that the Arrhenius plots for the first two processes have the same characteristics namely break at temperature around 18 degrees C, and Ea-58 kJ/mol at t > 18 degrees C. These data suggest that the velocity of ATP-depended vesiculation of erythrocytes is determined by the membrane permeability to monovalent cations. The appearance of methemoglobin in extracellular medium is characterized by a distinct value Ea(120 kJ/mol), although kinetics of this process is similar to vesiculation kinetics. Connection between considered processes and interrelation between ATP-depended vesiculation and spontaneous vesiculation are discussed.
Chernitskiĭ
E A
EA
Slobozhanina
E I
EI
Fedorovich
I E
IE
Novitskaia
G P
GP
D000110:Acetylcholinesterase / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001793:Blood Preservation; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D017136:Ion Transport; D007700:Kinetics; D011188:Potassium / Q000378:metabolism; D013696:Temperature
D000255:Adenosine Triphosphate; D000110:Acetylcholinesterase; D011188:Potassium
Vezikuliatsiia -eritrotsitov pri ikh khranenii i sviaz' ee s drugimi protsessami v kletke.