Crystallization and preliminary X-ray analysis of restriction endonuclease BamHI-DNA complex.
Restriction endonuclease BamHI from Bacillus amyloliquefaciens has been co-crystallized with a 12 bp DNA fragment that encompasses its recognition site. The co-crystals diffract to at least 1.95 A resolution and belong to space group P2(1)2(1)2(1). The unit cell parameters are a = 108.8 A, b = 81.9 A, c = 68.8 A, consistent with one complex in the crystallographic asymmetric unit. The direction of the DNA appears to be along the b axis. In order to achieve end to end stacking of DNA, the complex must lie on the screw axis along b. A self-rotation function has determined the directions of the non-crystallographic 2-fold axes.
Strzelecka
T
T
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.
Newman
M
M
Dorner
L F
LF
Knott
R
R
Schildkraut
I
I
Aggarwal
A K
AK
D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011485:Protein Binding
D004247:DNA; D015245:Deoxyribonuclease BamHI