Efficient adenovirus-mediated gene transfer into human blood monocyte-derived macrophages.
The efficiency of gene transfer into human blood monocyte-derived macrophages has been evaluated using a replication-defective adenovirus vector harboring a lac Z gene of E. coli as a reporter gene. Whereas, no beta-galactosidase activity was found in freshly infected purified monocytes, 40% to 80% of infected macrophages which derived from these monocytes showed a beta-galactosidase activity, 2 to 4 days after infection and lasted for at least 3 weeks. Moreover, beta-galactosidase activity was found in infected monocyte/macrophages 7 days after their injection into a human tumor preestablished in nude mice. These data indicate that it is possible to transfer and stably express a gene of potential therapeutical function into human monocyte-derived macrophages using an adenovirus vector.
Haddada
H
H
CNRS UA, Institut Gustave Roussy, Villejuif, France.
Lopez
M
M
Martinache
C
C
Ragot
T
T
Abina
M A
MA
Perricaudet
M
M
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors; D006651:Histocytochemistry; D006801:Humans; D007111:Immunity, Cellular; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008819:Mice, Nude; D009000:Monocytes / Q000382:microbiology*; D009374:Neoplasms, Experimental; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase