Inositol 1,4,5-trisphosphate receptor subtypes in adrenal glomerulosa cells.
Expression of the diverse subtypes of inositol 1,4,5-trisphosphate (InsP3) receptor (IP3R) was examined in rat adrenal glomerulosa cells. The polymerase chain reaction products were characterized by means of DNA sequencing and/or restriction enzyme mapping. The predominant subtype expressed is IP3R-1; its alternatively spliced variants containing and lacking segment S1 are present in comparable amounts. The expression level of IP3R-2 is about a quarter that of IP3R-1, whereas IP3R-3 is expressed at a very low level. Sodium depletion, a chronic physiological stimulus of glomerulosa cells, failed to influence the expression of IP3R-1, as measured by competitive polymerase chain reaction, and failed to modify the ratio of the different receptor subtypes, as studied with restriction enzyme mapping.
Enyedi
P
P
Department of Physiology, Semmelweis University of Medicine, Budapest, Hungary.
Szabadkai
G
G
Horváth
A
A
Szilágyi
L
L
Gráf
L
L
Spät
A
A
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D015870:Gene Expression; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012964:Sodium / Q000008:administration & dosage / Q000172:deficiency; D015384:Zona Glomerulosa / Q000378:metabolism*
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D012964:Sodium