Characterization of a monoclonal antibody resistant variant of MHV.
A monoclonal antibody resistant (MAR) variant of MHV was isolated after infection of hybridoma cells secreting the neutralizing and fusion-inhibiting monoclonal antibody, mAb 11F. The isolated variant was able to mediate syncytia formation even in the presence of high concentrations of mAb 11F. The S gene of the variant was cloned and sequenced. There were three nucleotide exchanges in comparison to the wild-type S gene, resulting in two amino acid alterations. However, both amino acid substitutions (at positions 255 and 1116) were located outside the binding site of mAb 11F.
Grosse
B
B
Institut für Virologie, Universität Würzburg.
Siddell
S G
SG
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D001254:Astrocytoma; D002459:Cell Fusion; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000235:genetics; D006825:Hybridomas; D007739:L Cells / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D064370:Spike Glycoprotein, Coronavirus; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins