Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked.
The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.
Danaher
R J
RJ
Department of Microbiology, University of Maryland, College Park 20742.
Petricoin
E F
EF
Stein
D C
DC
D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase; D004269:DNA, Bacterial / Q000235:genetics; D049308:Dioxygenases; D005798:Genes, Bacterial / Q000235:genetics*; D017930:Genes, Reporter; D008070:Lipopolysaccharides / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010105:Oxygenases / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase