Labeling of estradiol and testosterone alkyloxime derivatives with a europium chelate for time-resolved fluoroimmunoassays.
O-Carboxymethyl, O-(4aminobutyl)-, and O-(6-aminohexyl)-oximes of 6-oxoestradiol and testosterone were labeled using a described general labeling method with a europium chelate. A stable amide or thiourea bond was formed between the europium label and carboxyl or amino derivative of the steroid, respectively. These labeled "tracers" were purified and briefly tested in a competitive time-resolved fluoroimmunoassay using monoclonal antibodies raised against O-(carboxymethyloxime)-bovine serum albumin derivatives of estradiol and testosterone. Only buffer-based standards were used in a model system for the testing. These studies indicate only minor functional differences while using these antibodies due to the length of the spacer arm, and a higher sensitivity in the clinically relevant standard range was reached with the longer spacers.
Mikola
H
H
Wallac Chemical Laboratories, Turku, Finland.
Sundell
A C
AC
Hänninen
E
E
D000911:Antibodies, Monoclonal; D004958:Estradiol / Q000737:chemistry*; D005063:Europium; D015200:Fluoroimmunoassay; D007118:Immunoassay; D007553:Isotope Labeling; D010091:Oximes; D013386:Succinates; D013739:Testosterone / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D010091:Oximes; D013386:Succinates; D013739:Testosterone; D005063:Europium; D004958:Estradiol