Stable expression of gp80 (TRPM-2, clusterin), a secretory protein implicated in programmed cell death, in transfected BHK-21 cells.
The gp80 (TRPM-2, clusterin) cDNA cloned into an eucaryotic expression vector, was transfected into BHK-21 cells and stably transformed cell clones were obtained. Analysis of the gp80 glycoprotein complex produced in these cells demonstrated that the complex was glycosylated, proteolytically processed and secreted in a way similar to the gp80 glycoprotein complex expressed from the endogenous gene in MDCK cells. The analysis of the viability of the cells, the morphology and the state of the DNA in the transfected cells was unchanged when compared with the untransformed cells, demonstrating that the expression of the protein failed to elicit any signs of apoptosis in this system.
Pilarsky
C
C
Johannes-Gutenberg-Universität Mainz, Institut für Biochemie, Germany.
Haase
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W
Koch-Brandt
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C
D000818:Animals; D017209:Apoptosis; D002461:Cell Line, Transformed; D051152:Clusterin; D006224:Cricetinae; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008854:Microscopy, Electron; D018832:Molecular Chaperones; D008970:Molecular Weight; D014162:Transfection
D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones