[Thyrotropin receptor is expressed in non-thyroid tissues].
We have obtained TSH-R cDNA fragments from rat retro-orbital tissues and adipose tissue as well as FRTL5 cells by polymerase chain reaction methods. Nucleotide sequence of the non-thyroid cDNA was identical to that of the thyroid. Northern blot analysis has revealed that extremely high amount of TSH-R mRNA exist in the adipose tissue. TSH-R peptide antibody produced in rabbit, which stained recombinant TSH-R as 104 and 100 kDa proteins, also recognized the same M.W. bands in the adipose tissue and the retro-orbital tissues. These results suggest that TSH-R is expressed even in non-thyroid tissues.
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Third Department of Internal Medicine, University of Yamanashi Medical School.
Onaya
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D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004247:DNA / Q000032:analysis; D006801:Humans; D009915:Orbit / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D051381:Rats; D011989:Receptors, Thyrotropin / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D011989:Receptors, Thyrotropin; D004247:DNA