title: Prostaglandin secretion by guinea pig tracheal epithelial cells caused by eosinophil major basic protein.
abstract: We examined the effect of eosinophil major basic protein (MBP) on prostaglandin (PG) secretion from guinea pig tracheal epithelial (GPTE) cells. Primary cultures of GPTE cells were incubated with 10(-6) M MBP for up to 6 h and then stimulated with 10(-6) M bradykinin (BK). PGE2, 6-ketoprostaglandin F1 alpha (PGF1 alpha), PGF2 alpha, and thromboxane B2 (TxB2) concentrations in media were determined by enzyme-linked immunoabsorbent assay (EIA). Incubation with MBP for 6 h caused secretion of both PGE2 (17,614 +/- 4,416 vs. 1,426 +/- 555 pg/10(6) cells at baseline, P < 0.001, n = 7) and PGF2 alpha (20,303 +/- 5,724 vs. 3,790 +/- 1.075 pg/10(6) cells at baseline, P < 0.002, n = 7). Secretion of PGE2 and PGF2 alpha stimulated by MBP required at least 2 h. Incubation with MBP for 6 h also augmented the subsequent response to BK: PGE2 secretion was 29,215 +/- 6,853 vs. 3,445 +/- 1,041 pg/10(6) cells for BK alone (P < 0.0001), and PGF2 alpha secretion was 25,407 +/- 6,237 vs. 5,213 +/- 1,535 pg/10(6) cells for BK alone (P < 0.0001). MBP did not change 6-keto-PGF1 alpha and TxB2 secretion. Incubation of GPTE cells from seven animals with polylysine, a protein with mass and ion charge similar to MBP, for 2 h, both caused secretion of PGE2 (8,579 +/- 3,244 vs. 788 +/- 419 pg/10(6) cells at baseline, P < 0.01) and augmented the response to BK (12,732 +/- 4,788 vs. 1,653 +/- 680 pg/10(6) cells after BK alone, P < 0.005).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, University of Chicago, Illinois 60637.
authors.forename: S R
authors.initials: SR
authors.lastname: White
authors.forename: K S
authors.initials: KS
authors.lastname: Sigrist
authors.forename: S M
authors.initials: SM
authors.lastname: Spaethe
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D001920:Bradykinin / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D047091:Eosinophil Granule Proteins; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006168:Guinea Pigs; D011107:Polylysine / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D012260:Ribonucleases; D013936:Thymidine / Q000378:metabolism; D014132:Trachea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D011453:Prostaglandins; D011107:Polylysine; D012260:Ribonucleases; D001920:Bradykinin; D013936:Thymidine