Effect of inducers on the activity of glutathione S-transferase and other enzymes of the glutathione pathway in cultured human keratinocytes.
Known inducers of the hepatic glutathione (GSH) S-transferases were tested at the limits of their solubility as inducers of the enzyme in cultured human keratinocytes. Neither phenobarbital, trans-stilbene oxide, propylthiouracil, nor butylated hydroxyanisole increased GSH S-transferase activity or led to the appearance of alpha- or mu-forms of the enzyme, as judged by Western blotting. Only the pi-form of the enzyme was found before and after all treatments. Thus, the enzyme is not inducible in keratinocytes. However, 4 mM propylthiouracil did lead to a 50% increase in GSH reductase activity, and phenobarbital at 4 mM completely abolished GSH peroxidase and GSH reductase activity and led to a significant loss of viability.
Vessey
D A
DA
Liver Study Unit, Veterans Affairs Medical Center, San Francisco, CA 94121.
Lee
K H
KH
Blacker
K L
KL
Boyer
T D
TD
D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000187:drug effects; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000187:drug effects; D005980:Glutathione Reductase / Q000096:biosynthesis / Q000187:drug effects; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology; D011441:Propylthiouracil / Q000494:pharmacology; D013267:Stilbenes / Q000494:pharmacology
D013267:Stilbenes; D002083:Butylated Hydroxyanisole; D011441:Propylthiouracil; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; C025906:stilbene oxide; D010634:Phenobarbital