Clarification of subtle reciprocal rearrangements using fluorescence in situ hybridization.
Fluorescence in situ hybridization (FISH) using chromosome-specific DNA libraries as painting probes was applied in the analysis of six subtle, balanced chromosome rearrangements. Both fresh and older slides, some of which had been previously G-banded, were used to determine if FISH could identify unambiguously very small amounts of translocated material. Our results indicate that this procedure can clearly and precisely distinguish the specific components of extremely subtle translocations, in different cell types, such as leukocytes, aminocytes, and chorionic villus, and irregardless of preparation age. This ability makes FISH a valuable tool in clinical cytogenetics for the confirmation of preliminary G-banded karyotypes.
Sullivan
B A
BA
Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore.
Leana-Cox
J
J
Schwartz
S
S
D002878:Chromosomes, Human, Pair 1; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D002887:Chromosomes, Human, Pair 18; D002892:Chromosomes, Human, Pair 22; D002894:Chromosomes, Human, Pair 4; D002897:Chromosomes, Human, Pair 7; D002898:Chromosomes, Human, Pair 8; D002899:Chromosomes, Human, Pair 9; D015342:DNA Probes; D005260:Female; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic
D015342:DNA Probes