The mouse Mel-18 "RING-finger" gene: genomic organization, promoter analysis and chromosomal assignment.
The chromosome gene for mouse Mel-18 (mMel-18) protein has been isolated and characterized. The entire mMel-18 gene is composed of thirteen exons spanning about 15 kilobases, in which the protein is encoded by exons 5-13. The "RING-finger" motif of Mel-18 protein that displays a significant evolutionary resemblance to other RING-finger nuclear proteins is encoded by exons 5 and 6. Exon 13 encodes a C-terminal proline/serine-rich domain that is homologous to some transactivator proteins. Sequence analysis of the 5'-flanking region revealed the presence of potential binding sites for transcription factors such as SP-1, NF-1, NF-kappa B and c-myc/max. At least two major cap sites and three minor cap sites were identified by S1 mapping and primer extension analysis. We propose that the mMel-18 gene is regulated by two different types of promoters, the CAAT-TATA box promoter and the GC-rich TATA-less promoter. The 2.4 kb DNA fragment of the 5'-flanking region exhibited constitutive promoter activity when transfected into L cells. By the in situ hybridization method, the mMel-18 gene was assigned to mouse chromosome 10C3.
Asano
H
H
Division of Molecular Immunology, School of Medicine, Chiba University, Japan.
Ishida
A
A
Hasegawa
M
M
Ono
T
T
Yoshida
M C
MC
Taniguchi
M
M
Kanno
M
M
D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D005786:Gene Expression Regulation; D016259:Genes, myc; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D009838:Oligodeoxyribonucleotides; D063150:Polycomb Repressive Complex 1; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins; D012315:RNA Caps; D012097:Repressor Proteins; D016335:Zinc Fingers
C489421:Bmi1 protein, mouse; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; C489348:PCGF2 protein, human; C066476:Pcgf6 protein, mouse; D011518:Proto-Oncogene Proteins; D012315:RNA Caps; D012097:Repressor Proteins; D063150:Polycomb Repressive Complex 1