[The development and testing of an MTT method for assessing the reaction of peripheral blood lymphocytes to irradiation].
The semiautomatic MTT assay for evaluation of differences in the reaction of human donors' peripheral blood lymphocytes to ionizing radiation was developed. The stimulation conditions and period of cultivation were determined, and redox activity of lymphocytes and their coefficient of stimulation after irradiation with different doses were defined.
Kirsanova
V A
VA
Sergeeva
N S
NS
Sviridova
I K
IK
Chissov
V I
VI
Babarina
A V
AV
Kuriliak
O A
OA
Makhmurova
N T
NT
Pelevina
I I
II
D001782:Blood Donors; D002478:Cells, Cultured / Q000201:enzymology / Q000528:radiation effects; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004357:Drug Synergism; D005069:Evaluation Studies as Topic; D006801:Humans; D007376:Interleukin-2; D007958:Leukocyte Count / Q000528:radiation effects; D008214:Lymphocytes / Q000201:enzymology / Q000528:radiation effects*; D008722:Methods; D010084:Oxidation-Reduction / Q000528:radiation effects; D010088:Oxidoreductases / Q000097:blood / Q000528:radiation effects; D011836:Radiation Tolerance; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
D004396:Coloring Agents; D007376:Interleukin-2; D013778:Tetrazolium Salts; D013844:Thiazoles; D010088:Oxidoreductases; C022616:thiazolyl blue
Razrabotka i aprobatsiia MTT-metoda dlia otsenki reaktsii limfotsitov perifericheskoĭ krovi na obluchenie.