New insights suggesting a possible role of a heat shock protein 70-kD family-related protein in antigen processing/presentation phenomenon in humans.
A possible role of the peptide binding protein (PBP) 72/74 in antigen processing and presentation has been recently suggested in mice. In order to evaluate a possible analogous role of a PBP72/74-related protein in humans, immunoelectron microscope investigations, functional studies, and immunofluorescence analyses were performed on normal human peripheral antigen-presenting cells. We demonstrated that the determinant recognized by antiheat shock protein (HSP) 72/73 monoclonal antibody (MoAb) is constitutively expressed on the cell surface of monocytes as well as of B cells. Moreover, the capability of monocytes to present a recall antigen to T cells was significantly decreased when preincubated with an anti-HSP72/73 MoAb. These data add further strength to a potential role of a protein related to human PBP72/74 homologue in antigen processing and/or presentation. Finally, the capability of anti-HSP72/73 MoAb to impair the ability of fixed monocytes to present a synthetic peptide demonstrates that cell surface-localized PBP72/74-related protein could play a role in antigen presentation.
Manara
G C
GC
Department of Dermatology, University of Parma, Italy.
Sansoni
P
P
Badiali-De Giorgi
L
L
Gallinella
G
G
Ferrari
C
C
Brianti
V
V
Fagnoni
F F
FF
Ruegg
C L
CL
De Panfilis
G
G
Pasquinelli
G
G
D000911:Antibodies, Monoclonal / Q000276:immunology; D017951:Antigen Presentation; D006684:HLA-DR Antigens / Q000502:physiology; D006360:Heat-Shock Proteins / Q000502:physiology*; D006801:Humans; D008213:Lymphocyte Activation; D008970:Molecular Weight; D009000:Monocytes / Q000276:immunology / Q000648:ultrastructure; D013745:Tetanus Toxoid / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D006360:Heat-Shock Proteins; D013745:Tetanus Toxoid