Detection of prostatic-inhibin-like peptide in the cytoplasm of LNCaP cells, a human prostatic adenocarcinoma cell line.
Prostatic inhibin peptide (PIP) is a 94-amino acid peptide involved in various cellular functions. The concentration of this peptide changes with prostatic pathophysiology suggesting a role in various disease conditions; present study was undertaken to investigate the presence of this peptide in two human prostatic cell lines: LNCaP and PC3 cells. The LNCaP cells showed an intense intracellular fluorescence pattern after staining with rabbit-anti-PIP antiserum and FITC conjugated goat antirabbit-IgG, while the PC3 cells did not exhibit any fluorescence. There was no alteration in the concentration of PIP in LNCaP cells with or without supplementation of steroids in culture medium. Immunoblot analysis indicates similarities between PIP from LNCaP cells and that from the human seminal plasma. Thus, present study demonstrates the presence of PIP in a human prostatic cell line, i.e., LNCaP cells. Its intracellular concentration is androgen independent, and has a close similarity with PIP isolated from the human seminal plasma.
Hurkadli
K S
KS
Institute for Research in Reproduction (ICMR), Parel, Bombay, India.
Lokeshwar
B
B
Sheth
A R
AR
Block
N L
NL
D000230:Adenocarcinoma / Q000473:pathology*; D015153:Blotting, Western; D002460:Cell Line; D003593:Cytoplasm / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007265:Inhibins / Q000032:analysis*; D008297:Male; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000473:pathology*; D029584:Prostatic Secretory Proteins; D011863:Radioimmunoassay; D012661:Semen / Q000737:chemistry*; D013739:Testosterone / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D010455:Peptides; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; D013739:Testosterone; D007265:Inhibins