Gas chromatography/combustion/isotope-ratio mass spectrometry analysis of urinary steroids to detect misuse of testosterone in sport.
We propose a new confirmatory method for testosterone doping in sport. The present method in use, based on measuring the testosterone/epitestosterone (T/E) ratio in urine, may miss suspicious cases, or lead to reporting cases in which the high ratio is natural. Synthetic testosterone has a 13C abundance different from that of endogenous human testosterone. The connection of a gas chromatograph to an isotope-ratio mass spectrometer via a combustion interface allows the measurement of the corresponding characteristic value (delta /1000) for testosterone, its precursors, and its metabolites. To detect exogenous administration of testosterone, 30-40 mL of urine is sufficient.
Becchi
M
M
Service Central d'Analyse, CNRS, Vernaison, France.
Aguilera
R
R
Farizon
Y
Y
Flament
M M
MM
Casabianca
H
H
James
P
P
D000107:Acetylation; D002784:Cholesterol / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003687:Dehydroepiandrosterone / Q000652:urine; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D013256:Steroids / Q000652:urine*; D015813:Substance Abuse Detection; D013739:Testosterone / Q000652:urine*
D007202:Indicators and Reagents; D013256:Steroids; D013739:Testosterone; D003687:Dehydroepiandrosterone; D002784:Cholesterol