Soaking nitrocellulose blots in acidic buffers improves the detection of bound antibodies without loss of biological activity.
Hoffman
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Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
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D000143:Acids; D000906:Antibodies / Q000032:analysis*; D002021:Buffers; D003101:Collodion; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing; D007700:Kinetics; D008567:Membranes, Artificial; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D012685:Sepharose
D000143:Acids; D000906:Antibodies; D002021:Buffers; D007457:Iodine Radioisotopes; D008567:Membranes, Artificial; D011506:Proteins; D003101:Collodion; D012685:Sepharose