title: Production of IgG monoclonal antibodies to the tumor-associated antigen, CA-195.
abstract: The isotype of a monoclonal antibody is closely associated with its biologic activity. Certain immunoglobulin subclasses are more effective than others regarding their ability to execute complement-mediated lysis of cells, antibody-dependent cell-mediated cytotoxicity, and tumor localization. Many potential targets for cancer immunotherapy are tumor-associated antigens with high percentages of carbohydrate. Immunizations of mice with carbohydrate antigens usually produce IgM and IgG3 antibodies. The use of different adjuvants in immunization protocols has been associated with the induction of isotype-specific antibody responses. In experiments reported here, we compare the use of four different adjuvants on the generation of an IgG immune response to the carbohydrate-rich, tumor-associated antigen, CA-195. We report the production of IgG1 and IgG2a monoclonal antibodies (mAbs) to CA-195.
authors.affiliation: Hybritech Incorporated, San Diego, CA 92196-9006.
authors.forename: K
authors.initials: K
authors.lastname: Kuus-Reichel
authors.forename: C L
authors.initials: CL
authors.lastname: Knott
authors.forename: R T
authors.initials: RT
authors.lastname: McCormack
authors.forename: M S
authors.initials: MS
authors.lastname: Guido
authors.forename: A
authors.initials: A
authors.lastname: Beebe
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D007983:Lewis Blood Group Antigens / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A
chemical_list: D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; C081241:CA 195 antigen; D007074:Immunoglobulin G; D007983:Lewis Blood Group Antigens