The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner.
In animals, MAP kinase plays a key role in growth factor-stimulated signalling and in mitosis. The isolation of a Medicago sativa cDNA clone MsK7 which shows 52% identity to animal MAP kinases is reported. The deduced protein sequence shows all the important structural features of MAP kinases and also contains the highly conserved Thr-183 and Tyr-185 residues. Northern analysis of synchronized alfalfa cells showed that the MsK7 kinase gene is expressed at low levels in G1 phase but at higher levels in S and G2 phases of the cell cycle. In the plant, only stems and roots were found to contain MAP kinase MsK7 mRNA. Southern and PCR analyses indicated that alfalfa contains at least four highly related MAP kinase genes.
Jonak
C
C
Institute of Microbiology and Genetics, University of Vienna, Austria.
Páy
A
A
Bögre
L
L
Hirt
H
H
Heberle-Bors
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D002453:Cell Cycle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D015723:Gene Library; D000455:Medicago sativa / Q000235:genetics*; D020928:Mitogen-Activated Protein Kinases; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D010940:Plant Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C084057:MMK1 protein, Medicago sativa; D020928:Mitogen-Activated Protein Kinases