A rapid, automated microtiter assay for measuring neutralization of HIV-1.
A sensitive, rapid, and quantitative ELISA for p24 is described, which can be used as the read-out to test for HIV-1 neutralization in the syncytium-forming microassay and can replace the counting of syncytia under the microscope. This assay can be used reliably for divergent strains of HIV-1, including those that do not induce syncytia. The new read-out permits evaluation of neutralization at 3 days rather than 5 days. Using this assay, the neutralizing activity of several new and previously described human monoclonal antibodies against HIV-1 was characterized.
Laal
S
S
Veterans Affirs Medical Center, New York, New York 10010.
Burda
S
S
Sharpe
S
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Zolla-Pazner
S
S
D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015726:Giant Cells / Q000473:pathology; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D009500:Neutralization Tests
D016655:HIV Core Protein p24