Glu-255 outside the predicted ChvE binding site in VirA is crucial for sugar enhancement of acetosyringone perception by Agrobacterium tumefaciens.
Transcriptional activation of the Agrobacterium tumefaciens vir regulon is regulated by phenolics such as acetosyringone (AS), certain monosaccharides, and acidic conditions produced by wounded plant cells. The transmembrane protein VirA acts as an environmental sensor, mediating signal transduction upon perception of these stimuli. Although the periplasmic domain of VirA is not absolutely required for AS-dependent vir gene induction, it is needed for interactions with the periplasmic sugar-binding protein ChvE that result in sugar-induced enhancement of phenolic sensitivity. In this report, we demonstrate that mutations within the periplasmic domain but outside the predicted ChvE binding region can drastically alter the sensitivity of VirA to As. Using site-directed mutagenesis, we have characterized the roles of three individual amino acids in sugar-dependent AS sensitivity and have correlated the induction phenotype with the tumorigenic capacity of strains expressing mutant versions of VirA. Substitution of leucine for Glu-255 abolishes sugar enhancement while replacement with aspartic acid results in a wild-type phenotype. This residue lies outside the predicted ChvE binding site and thus identifies a new region of the VirA periplasmic domain crucial for the enhancement of vir gene induction by carbohydrates. In the absence of inducing sugar, wild-type VirA protein appears to be subject to some form of inhibition that suppresses the maximal level of transcriptional activation; deletions within the periplasmic region relieve this suppression.
Banta
L M
LM
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
Joerger
R D
RD
Howitz
V R
VR
Campbell
A M
AM
Binns
A N
AN
D000098:Acetophenones / Q000494:pharmacology; D016960:Agrobacterium tumefaciens / Q000472:pathogenicity / Q000502:physiology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004305:Dose-Response Relationship, Drug; D015964:Gene Expression Regulation, Bacterial; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000494:pharmacology; D016297:Mutagenesis, Site-Directed; D033902:Periplasmic Binding Proteins; D011485:Protein Binding; D015398:Signal Transduction / Q000235:genetics*; D013329:Structure-Activity Relationship; D015533:Transcriptional Activation; D014774:Virulence; D037521:Virulence Factors
D000098:Acetophenones; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D009005:Monosaccharides; D033902:Periplasmic Binding Proteins; D037521:Virulence Factors; C051667:acetosyringone