[Molecular characterization of the transferable resistance to amikacin in Enterobacteriaceae strains isolated from hospital infections].
Sixty-three amikacin resistant strains of Enterobacteriaceae isolated in three hospitals from Santiago, between 1988 and 1990 were included in this study. The strains were multiresistant and harbored 1-5 plasmids. Fifty six isolates (88.9%) transferred amikacin resistance to E coli C600 receptor strain by conjugation. The transconjugants acquired a 11 kilobase-pair or a larger plasmid. The plasmids also encoded resistance to kanamycin, tobramycin, streptomycin and ampicillin. A DNA probe from the gene AAC (6')-I encoding an aminoglycoside 6'-N-acetyltransferase, AAC (6')-I hybridized in Southern blot with plasmid DNA of the 10 Enterobacteriaceae strains tested, but not with the plasmid DNA of 8 amikacin resistant clinical strains of A baumannii. The results indicate that amikacin resistance in Enterobacteriaceae is due mainly to conjugative plasmids encoding an AAC (6')-I.
Martínez
M A
MA
Departamento de Preclínicos, Facultad de Medicina, Universidad de Chile, Campus Occidente.
Pinto
M E
ME
D000583:Amikacin / Q000494:pharmacology*; D003227:Conjugation, Genetic; D003428:Cross Infection / Q000382:microbiology; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D004755:Enterobacteriaceae / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D010957:Plasmids / Q000187:drug effects*
D000583:Amikacin
Caracterización a nivel molecular de la resistencia transferible a amikacina en cepas de Enterobacteriaceae aisladas de infecciones intrahospitalarias.