Brain-specific prostaglandin D2 synthetase mRNA is dependent on thyroid hormone during rat brain development.
We have previously described several cDNA clones whose expression is affected by thyroid hormone during rat brain development. We now report the identification of one of these, the E2 clone, as the brain-specific prostaglandin D2 (PGD2) synthetase gene. Sequence comparison shows a nearly complete identity between the 356 nucleotides of the E2 clone and nucleotides 403 to 759 of PGD2 synthetase cDNA. The pattern of E2 expression corresponds to that expected for brain specific PGD2 synthetase gene, i.e. the corresponding mRNA is not detected in any other tissue analyzed apart of the brain, and it was present at different levels in all brain regions. Hypothyroidism decreased E2 mRNA concentrations in cerebral cortex and cerebellum. Control of the level of expression of PGD2 synthetase gene may contribute the complex effects of thyroid hormone on brain development and function.
García-Fernández
L F
LF
Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Iñiguez
M A
MA
Rodríguez-Peña
A
A
Muñoz
A
A
Bernal
J
J
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects / Q000254:growth & development*; D015971:Gene Expression Regulation, Enzymologic; D007037:Hypothyroidism / Q000378:metabolism*; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000096:biosynthesis / Q000235:genetics*; D054834:Lipocalins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D012689:Sequence Homology, Nucleic Acid; D013963:Thyroid Hormones / Q000494:pharmacology*; D013965:Thyroidectomy
D054834:Lipocalins; D012333:RNA, Messenger; D013963:Thyroid Hormones; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase