Effects of promoter, intron and enhancer elements on transient gene expression in sugar-cane and carrot protoplasts.
Various chimaeric promoter regions coupled to the uidA beta-glucuronidase gene were evaluated for transient expression strength following electroporation into sugar-cane (monocot) and carrot (dicot) protoplasts. Multiple enhancer elements increased expression in sugar-cane, by up to 400-fold for the artificial Emu promoter relative to the CaMV 35S promoter. The relative expression strengths of promoters varied substantially between the species. Sugar-cane also differed in some respects from previously tested species in the family Poaceae. For example, in sugar-cane the nopaline synthase and CaMV 35S promoters were of equivalent strength, and insertion of Adh1 intron 1 into the 5' transcribed region decreased expression strength.
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Bureau of Sugar Experiment Stations, Brisbane, Australia.
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D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D017343:Genes, Plant; D007438:Introns; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000378:metabolism*; D014170:Transformation, Genetic; D014675:Vegetables