Comparison of the Rev transactivation of feline immunodeficiency virus in feline and non-feline cell lines.
The Rev protein of feline immunodeficiency virus (FIV) differentially transactivates the expression of viral structural proteins by allowing the accumulation of unspliced and singly spliced viral mRNA in cytoplasm via the Rev response element (RRE) at the end of env. To investigate the role of rev gene of FIV for the virus life cycle and cell tropism, we constructed the Rev expression plasmids, and functional activity of the Rev was assayed by using chloramphenicol acetyltransferase (CAT) assay system in feline and non-feline cell lines. Although the FIV Rev protein showed high transactivity to result in enhanced CAT production in a feline cell line, the productions of the CAT in non-feline cell lines were significantly lower than that in the feline cell line. These results indicate that specific cellular factor(s) present in feline cell line is required for the FIV Rev full-action and also suggest that the Rev action plays one of the important roles in determining the FIV cell tropism.
Tomonaga
K
K
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Miyazawa
T
T
Kawaguchi
Y
Y
Kohmoto
M
M
Inoshima
Y
Y
Mikami
T
T
D000818:Animals; D002415:Cats; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000378:metabolism; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003110:Colonic Neoplasms; D015694:Gene Products, rev / Q000096:biosynthesis / Q000378:metabolism*; D015752:Genes, env; D015757:Genes, rev; D006367:HeLa Cells; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics*; D007668:Kidney; D016366:Open Reading Frames; D010957:Plasmids; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013045:Species Specificity; D015533:Transcriptional Activation; D014162:Transfection; D014407:Tumor Cells, Cultured
D015694:Gene Products, rev; D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase