title: Handling and/or saline injections alter basal and morphine-evoked changes in dopamine metabolites in the striatum and nucleus accumbens of rats.
abstract: The effect of IP saline injections on basal dopamine (DA), homovanillic acid (HVA), and dihydroxyphenylacetic acid (DOPAC), and morphine-evoked levels of DA, DOPAC, and HVA in the striatum (STR) and nucleus accumbens (NAC) of female Sprague-Dawley rats was quantified, using in vivo microdialysis. In the STR, saline injections twice daily over 3 days, then once on day 4 (2x /day group), resulted in higher basal DOPAC and HVA levels compared to unhandled rats (naive group), or rats injected with saline once daily for 4 days (1x /day group). DOPAC and HVA were greater after IP injection of 30 mg/kg morphine in the saline 2x /day group compared to the saline 1x /day and naive groups. In the NAC, DOPAC was higher after injection of morphine in saline-pretreated rats, compared to naive rats. These results suggest that handling and/or IP saline injections affect basal and morphine-evoked changes in DA metabolites in the STR, and morphine-evoked DOPAC in the NAC.
authors.affiliation: Department of Pharmacology and Toxicology, Albany Medical College, NY 12208.
authors.forename: D W
authors.initials: DW
authors.lastname: Johnson
authors.forename: S D
authors.initials: SD
authors.lastname: Glick
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D006234:Handling, Psychological; D006719:Homovanillic Acid / Q000378:metabolism; D007267:Injections; D017551:Microdialysis; D009020:Morphine / Q000494:pharmacology*; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D012996:Solutions; D015102:3,4-Dihydroxyphenylacetic Acid; D009020:Morphine; D004298:Dopamine; D006719:Homovanillic Acid