Perturbation of the immunosuppressive action of glucocorticoids in rat thymocytes by liposoluble extracts of serum from AIDS patients.
Liposoluble extracts of serum from healthy men and AIDS patients (stages IVC1 and IVD by CDC criteria) inhibited the incorporation of [3H]thymidine into isolated rat thymocytes, but AIDS extracts were less inhibitory, requiring 1.8 times more cortisol in the AIDS extracts than in the healthy extracts to inhibit [3H]thymidine incorporation by 50%. Although the total serum extracts from AIDS patients contained 1.7 times more cortisol than the extracts from healthy controls, the AIDS extracts decreased the binding affinity (Ka) of [3H]dexamethasone to rat thymus glucocorticoid receptors by 50% less than the healthy control extracts. The present study seems to indicate that a substance(s) can be extracted from the serum of AIDS patients that attenuates the inhibitory effect of cortisol on thymocyte proliferation and interferes with the binding of cortisol to the glucocorticoid receptor.
Sumida
C
C
INSERM U224 affiliated with CNRS, Faculté de Médecine Xavier Bichat, Paris, France.
Vallette
G
G
Thobie
N
N
Nunez
E A
EA
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D012995:Solubility; D013936:Thymidine; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects*
D005230:Fatty Acids, Nonesterified; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D013936:Thymidine; D006854:Hydrocortisone