Effects of acute ethanol administration on the uptake of 59Fe-labeled transferrin by rat liver and cerebellum.
The uptake of iron by the liver and cerebellum was measured in rats using [59Fe]transferrin. An acute ethanol load (50 mmol/kg body wt., i.p.) elicited a significant increase in the hepatic and cerebellar non-heme iron concentration. The uptake of 59Fe by the liver and the cerebellum was significantly greater in the ethanol-treated rats than in control animals. The administration of allopurinol prior to the ethanol load prevented the changes in liver and cerebellar non-heme iron content. Moreover pretreatment with allopurinol reduced the ethanol-induced enhancement of 59Fe uptake by the liver and completely prevented the changes in 59Fe uptake by the cerebellum. These effects of allopurinol lead us to suggest that oxygen-derived free radicals are involved in the ethanol-induced disturbances of iron uptake both at the hepatic and cerebellar level.
Rouach
H
H
Department of Biomedical Research on Alcoholism, Biomédicale des Saints-Pères, Paris, France.
Houze
P
P
Gentil
M
M
Orfanelli
M T
MT
Nordmann
R
R
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism; D004347:Drug Interactions; D000431:Ethanol / Q000633:toxicity*; D006400:Hematocrit; D007501:Iron / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism*
D014168:Transferrin; D000431:Ethanol; D000493:Allopurinol; D007501:Iron