Structure determination of the disialylated poly-(N-acetyllactosamine)-containing O-linked carbohydrate chains of equine chorionic gonadotropin.
The disialylated poly-(N-acetyllactosamine)-containing O-linked oligosaccharide alditols, released by alkaline borohydride treatment of the enzymically N-deglycosylated beta-subunit of equine chorionic gonadotropin, were purified by fast protein liquid chromatography (FPLC) on Mono Q and analysed by fast ion bombardment mass spectrometry (FAB-MS) and 1H-NMR spectroscopy. The identified oligosaccharide alditols have the following structure: [Formula: see text]
Hokke
C H
CH
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht, The Netherlands.
Roosenboom
M J
MJ
Thomas-Oates
J E
JE
Kamerling
J P
JP
Vliegenthart
J F
JF
D000606:Amino Sugars / Q000737:chemistry; D000818:Animals; D002240:Carbohydrate Sequence; D006063:Chorionic Gonadotropin / Q000737:chemistry*; D004376:Galactitol / Q000031:analogs & derivatives; D006023:Glycoproteins / Q000737:chemistry*; D006736:Horses; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D017421:Sequence Analysis; D012794:Sialic Acids / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000606:Amino Sugars; D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D009844:Oligosaccharides; D012794:Sialic Acids; D004376:Galactitol; C000458:N-acetyllactosamine