title: Effect of culture from the zygote stage on the metabolism of glucose and glutamine by 2-cell embryos and blastocysts recovered from outbred or F1 hybrid female mice.
abstract: The oxidation and incorporation of glucose and glutamine by embryos derived from cultured zygotes was compared with the utilization of these substrates by embryos recovered directly from the reproductive tract of pregnant females. The oxidation of glutamine was greater at the blastocyst stage than at the 2-cell stage. Embryos derived from outbred females (Qs) were less active in the oxidation of glutamine than those from hybrid (B10D2F1) females and development in culture was detrimental to this oxidation, especially in blastocysts from the outbred stock. The oxidation of glutamine was stimulated by the presence of glucose at the 2-cell stage but reduced by its presence at the blastocyst stage. Maternal genotype had no effect on the oxidation of glucose at either the 2-cell or blastocyst stage, and only at the blastocyst stage was there evidence of a detrimental effect of culture. The oxidation of glucose was stimulated by the presence of glutamine at the 2-cell stage but depressed by its addition at the blastocyst stage. Incorporation of glutamine increased with development, but this was reduced at the blastocyst stage by development in culture, especially if the blastocysts were derived from outbred females. Incorporation of glucose also increased with development. At the 2-cell stage, culture reduced incorporation of this substrate, especially into the acid-soluble fraction of embryos from outbred females. In blastocysts, incorporation of glucose into the acid-insoluble fraction was depressed by culture and in embryos from outbred females. In contrast to glucose oxidation, incorporation of glucose into the acid-soluble fraction was reduced by the addition of glutamine at the 2-cell stage but increased by its addition at the blastocyst stage.
authors.affiliation: School of Veterinary Studies, Murdoch University, Australia.
authors.forename: Z F
authors.initials: ZF
authors.lastname: Du
authors.forename: R G
authors.initials: RG
authors.lastname: Wales
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D051379:Mice; D009924:Organ Culture Techniques / Q000379:methods; D015053:Zygote
chemical_list: D003470:Culture Media; D005973:Glutamine; D005947:Glucose