title: Assays for adjuvanticity of new formulations and of carrier proteins for inducing antibody responses to selected immunogens in the squirrel monkey Saimiri sciureus.
abstract: Different ways to improve antibody (Ab) responses following immunizations with selected antigens (TT and HSVgD) were investigated, and thus new adjuvant formulations and carrier molecules in a non-human primate experimental host, the squirrel monkey Saimiri sciureus, were assayed. Both quantitative and qualitative humoral responses were determined by means of radio-immunoassays using monoclonal Ab directed at Saimiri IgG. First, the adjuvanticity of the Syntex (SAF-1) adjuvant and of five new adjuvant formulations were assessed towards the selected Ag. This indicated that all the adjuvants induced similar antigen-specific Ab responses, although the adjuvants could modify to some extent the pattern of the qualitative Ab response. Second, we evaluated an adjuvant-free vaccine approach using a synthetic Ag from the circumsporozoite protein of Plasmodium falciparum as immunogen, this Ag being coupled to purified protein derivative (PPD) or to a recombinant heat shock protein from Mycobacterium tuberculosis. These constructs led to good antibody responses as well as an excellent memory effect. Bacille Calmette-Guérin (BCG) priming was required in conjunction with PPD as a carrier molecule to allow homogeneous Ab responses, whereas the heat shock protein construct gave a less homogeneous Ab response regardless of whether a BCG priming was done. We, in addition, discuss the relevance of Saimiri monkeys as experimental models for studies directed at evaluating the immunogenicity of further vaccine candidates.
authors.affiliation: Laboratoire d'Immunologie Parasitaire, Institut Pasteur de la Guyane Francaise, Cayenne, France.
authors.forename: R
authors.initials: R
authors.lastname: Perraut
authors.forename: P
authors.initials: P
authors.lastname: Chouteau
authors.forename: E
authors.initials: E
authors.lastname: Bourreau
authors.forename: B
authors.initials: B
authors.lastname: Bonnemains
authors.forename: O
authors.initials: O
authors.lastname: Garraud
mesh_terms: D000276:Adjuvants, Immunologic / Q000032:analysis*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology; D002352:Carrier Proteins / Q000032:analysis*; D006360:Heat-Shock Proteins / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology; D011863:Radioimmunoassay; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012453:Saimiri; D013745:Tetanus Toxoid / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology; D001616:beta-Galactosidase / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000953:Antigens, Protozoan; D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D013745:Tetanus Toxoid; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; D001616:beta-Galactosidase