Expression of Na+-independent isoleucine transport activity from rat brain in Xenopus laevis oocytes.
Poly(A)+ RNA from C6-BU-1 rat glioma cells and rat astroglial cells induced isoleucine transport activity when injected into Xenopus laevis oocytes. The Na+-independent component of isoleucine transport was inhibited by leucine, phenylalanine and 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid (BCH) but neither by methylaminoisobutyric acid (MeAIB) nor lysine. A Km value of approx. 100 microM was determined for the Na+-independent transport of isoleucine. These data are in accordance with expression of a system L like transporter. By injection of size fractionated poly(A)+ RNA a length of approx. 1.9 kb was determined for the pertinent mRNA.
Bröer
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S
Physiologisch-Chemisches Institut der Universität, Tübingen, Germany.
Bröer
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A
Hamprecht
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B
D026905:Amino Acid Transport Systems; D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D007532:Isoleucine / Q000378:metabolism*; D007700:Kinetics; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014982:Xenopus laevis
D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D012333:RNA, Messenger; D007532:Isoleucine; D011061:Poly A; D012964:Sodium