Expression of tissue kallikrein in normal and SV40-transfected human endometrial stromal cells.
The polymerase chain reaction with specific tissue kallikrein primers was utilized to demonstrate the presence of tissue kallikrein mRNA in human endometrial stromal cells. Enzymatic analysis measured with a specific tripeptide nitroanilide substrate demonstrated the presence of tissue kallikrein in the conditioned medium obtained from both normal stromal cells and stromal cells transfected with an origin-defective temperature-sensitive SV40 large T antigen. The transfected stromal cell supernatant exhibited approximately twice as much tissue kallikrein activity as normal stromal cells at 60-100% of cell confluence. The release of tissue kallikrein from transfected stromal cells was confirmed by Western blot analysis and [35S]-methionine incorporation into a 35-kD protein which retains tissue kallikrein activity. These results demonstrate for the first time the expression and secretion of tissue kallikrein in human endometrial stromal cells and provide evidence of possible involvement of tissue kallikrein in cell transformation.
Wu
H F
HF
Dental Research Center, School of Dentistry, University of North Carolina, Chapel Hill 27599-3683.
Xu
L H
LH
Jenzano
J W
JW
Rinehart
C A
CA
Kaufman
D G
DG
Lundblad
R L
RL
D001483:Base Sequence; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002472:Cell Transformation, Viral; D017931:DNA Primers / Q000737:chemistry; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007610:Kallikreins / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D013539:Simian virus 40
D017931:DNA Primers; D012333:RNA, Messenger; D007610:Kallikreins