Mechanisms of transthyretin amyloidogenesis. Antigenic mapping of transthyretin purified from plasma and amyloid fibrils and within in situ tissue localizations.
Transthyretin (TTR) is the major amyloid fibril protein in senile systemic amyloidosis and in several forms of familial amyloidoses. However, the internal organization of the fibrils is virtually unknown. It is not known whether the structure of the TTR molecules is substantially altered within the fibrils. In this study we used various antigenic mapping procedures to determine whether major antigenic sites differ between normal TTR, ATTR (TTR from amyloid fibrils), and in situ amyloid fibrils. Antigenic mapping was achieved using standard immunological procedures (ie, ELISA, Western blot, and immunohistochemistry), synthetic peptides of the TTR molecule, antisera against these synthetic peptides and against normal TTR, ATTR, and alkali-degraded amyloid fibrils. Our results show that the antigenic sites on normal plasma TTR include the AB loop and the CD loop. The amino acid sequences associated with these loops are present on the outside of the TTR molecule. Antiserum against beta-strand H reacted only with TTR in amyloid fibrils and ATTR but not with normal plasma TTR or TTR in the islets of Langerhans. Our results suggest that there is an altered configuration of TTR within amyloid fibrils when compared with plasma TTR.
Gustavsson
A
A
Department of Pathology I, University of Linköping, Sweden.
Engström
U
U
Westermark
P
P
D000595:Amino Acid Sequence; D000682:Amyloid / Q000032:analysis* / Q000276:immunology / Q000378:metabolism*; D000686:Amyloidosis / Q000378:metabolism; D015153:Blotting, Western; D004247:DNA / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007515:Islets of Langerhans / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D016874:Neurofibrillary Tangles / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D011228:Prealbumin / Q000032:analysis* / Q000276:immunology / Q000378:metabolism*
D000682:Amyloid; D007106:Immune Sera; D011228:Prealbumin; D004247:DNA