Effects of transforming growth factor beta and interleukin-1 beta on [3H]thymidine incorporation by human articular chondrocytes in vitro.
This is a study of the regulation of human articular chondrocyte proliferation by transforming growth factor beta (TGF beta) and interleukin-1 beta (IL-1 beta) in vitro. Human articular chondrocytes were cultured at different cell densities on plastic and on a collagen substratum, in the presence and absence of serum. The effects of TGF beta and IL-1 beta on proliferation of chondrocytes, as determined by [3H]thymidine incorporation, under these conditions of culture were examined. TGF beta was found to have both stimulatory and inhibitory effects on chondrocytes in vitro. Interactions between TGF beta and growth factors present in serum influence the modulation of chondrocyte proliferation by TGF beta. IL-1 beta caused a significant reduction of the TGF beta-stimulated increase in chondrocyte proliferation. The complex inter-relationships between TGF beta and IL-1 beta on chondrocytes have implications for cartilage repair.
Frazer
A
A
Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, UK.
Bunning
R A
RA
Russell
R G
RG
D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D007375:Interleukin-1; D016212:Transforming Growth Factor beta; D004247:DNA; D013936:Thymidine