Biological inactivation of adhering Listeria monocytogenes by listeriaphages and a quaternary ammonium compound.
The use of listeriaphages as a means of disinfecting contaminated stainless-steel and polypropylene surfaces was investigated. Surfaces artificially contaminated with L. monocytogenes 10401 and 8427 were sanitized with suspensions of listeriaphages (H387, H387-A, and 2671), all belonging to the Siphoviridae family. Phage suspensions at concentrations of up to 3.5 x 10(8) PFU/ml were at least as efficient as a 20 ppm solution of a quaternary ammonium compound (QUATAL) in reducing L. monocytogenes populations. A synergistic activity was observed when two or more phages were used in combination and when phages were suspended in QUATAL. The biological activity of the three phages was not affected by QUATAL concentrations of 50 ppm and a contact time of 4 h.
Roy
B
B
Dairy Research Center (STELA), Faculty of Agriculture, Université Laval, Ste-Foy, Québec, Canada.
Ackermann
H W
HW
Pandian
S
S
Picard
G
G
Goulet
J
J
D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology; D001435:Bacteriophages / Q000502:physiology*; D004203:Disinfection / Q000379:methods*; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D011126:Polypropylenes; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013193:Stainless Steel; D013499:Surface Properties
D011126:Polypropylenes; D000644:Quaternary Ammonium Compounds; D013193:Stainless Steel