Interference of immunoglobulin G (IgG) antibodies in IgA antibody determinations of Chlamydia pneumoniae by microimmunofluorescence test.
In the microimmunofluorescence test for measuring immunoglobulin A (IgA) antibodies against Chlamydia pneumoniae, removal of interfering IgG antibodies made IgA antibody reactivity patterns in 952 serum samples easier to interpret, prozone effects disappeared, and titers increased, especially in the sera with high IgG titers. IgA rheumatoid factors did not interfere in the assay.
Jauhiainen
T
T
Department of Virology, University of Helsinki, Finland.
Tuomi
T
T
Leinonen
M
M
Kark
J D
JD
Saikku
P
P
D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D002690:Chlamydia Infections / Q000175:diagnosis / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012217:Rheumatoid Factor / Q000097:blood
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012217:Rheumatoid Factor