title: A cDNA clone for a pathogenesis-related protein 1 from barley.
abstract: A barley cDNA clone (PRb-1) corresponding to an mRNA differentially induced in resistant compared to susceptible barley cultivars by powdery mildew infection was isolated and characterised. The deduced amino acid sequence revealed 24 amino acids comprising the signal peptide and 140 amino acids of the mature peptide (15 kDa). This showed close homology to PR-1-like proteins, which have been isolated from maize, tobacco, tomato and Arabidopsis thaliana. Northern blot analysis showed accumulation of the corresponding mRNA 12 h after inoculation of resistant barley cultivars with Erysiphe graminis. Increased expression of the PRb-1 gene was also observed in resistant compared with near-isogenic susceptible barley plants following treatment with ethylene, salicylic acid, methyl jasmonate and 2,6-dichloro-isonicotinic acid.
authors.affiliation: Department of Biochemistry, University of Queensland, Brisbane, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Muradov
authors.forename: L
authors.initials: L
authors.lastname: Petrasovits
authors.forename: A
authors.initials: A
authors.lastname: Davidson
authors.forename: K J
authors.initials: KJ
authors.lastname: Scott
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D015723:Gene Library; D001467:Hordeum / Q000235:genetics*; D007539:Isonicotinic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D007539:Isonicotinic Acids; D010940:Plant Proteins; D012459:Salicylates; C053376:pathogenesis-related proteins, plant; C077261:2,6-dichloroisonicotinic acid; D020156:Salicylic Acid