Changes on protein expression associated with salinity tolerance in Brassica cell cultures.
The synthesis of proteins from salt-tolerant Brassica oleracea L. var. botrytis L. subvar. cauliflora (Gars.) DC. (cauliflower) cell cultures is modified in relation to controls in several features. There are nine newly induced polypeptides in tolerant cultures (absent in control conditions). Some of them are only present under low salt levels (85 mM NaCl). Another group seems to be representative of moderate and high salt levels (170 and 255 mM NaCl), and a third group is present in all the salt conditions tested. On the other hand, the synthesis of most of the polypeptides present in control conditions is modified in salt-tolerant cultures by increasing, decreasing or stopping their synthesis in any of the tested conditions. The relationship between these changes in Brassica and other plant systems is discussed.
Martín
J P
JP
Departamento de Biologia Vegetal, Universidad Politecnica de Madrid, Spain.
Elavummoottil
O C
OC
Moreno
M L
ML
D001937:Brassica / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004361:Drug Tolerance; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects*; D009994:Osmolar Concentration; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*; D013382:Subtraction Technique
D010940:Plant Proteins; D012965:Sodium Chloride