CT-boxes are involved in control of the rat insulin II gene expression.
Expression of the rat insulin II gene is controlled mainly at the level of transcription initiation by multiple factors binding to specific cis-acting DNA-elements in the regulatory region. We have shown that two elements (CT-motifs) located between nucleotides -83 and -76 (CT-1) and -204 and -197 (CT-2) are involved in transcriptional regulation in the insulin-producing cell line HIT M2.2.2. Transient expression analysis of 5'-deletion as well as block replacement mutants revealed that CT-1 and CT-2 are mutational sensitive. Gel mobility shift assays showed that both motifs bind similar nuclear factors. Our results suggest the involvement of a third CT-motif located directly upstream of CT-2 on the complementary strand.
Rommel
C
C
Institut für Biochemie, Medizinische Fakultät, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
Leibiger
I B
IB
Leibiger
B
B
Walther
R
R
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase; D006224:Cricetinae; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D007328:Insulin / Q000235:genetics* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017384:Sequence Deletion; D014162:Transfection
D004268:DNA-Binding Proteins; D007328:Insulin; D015500:Chloramphenicol O-Acetyltransferase