[Cloning and characterization of the human thyrotropin-releasing hormone receptor: regulation of thyrotropin-releasing hormone receptor mRNA levels by thyroid hormone].
1) We successfully cloned a human thyrotropin-releasing hormone receptor (TRH-R) gene. The human TRH-R consists of 398 amino acids and is a member of the G protein-coupled receptor family. The gene has a single intron in the coding sequence. Although highly homologous to the rat and mouse TRH-Rs, the human TRH-R has several differences in the carboxyl-terminal structure. 2) We next studied the effect of thyroid status on TRH-R mRNA levels both in vivo and in vitro. TRH-R mRNA levels in hypothyroid rats were increased significantly, while no significant changes were observed in vitro. The data indicate that thyroid hormone may regulate TRH-R mRNA levels in different manners in vitro and in vivo.
Yamada
M
M
First Department of Internal Medicine, Gunma University, School of Medicine.
Mori
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000235:genetics*; D013963:Thyroid Hormones / Q000502:physiology*
D018076:DNA, Complementary; D012333:RNA, Messenger; D018025:Receptors, Thyrotropin-Releasing Hormone; D013963:Thyroid Hormones