Sequence of the 5'-flanking region of the rat c-fos proto-oncogene.
The proto-oncogene c-fos is an immediate-early gene that becomes activated by a wide variety of extracellular stimuli. Since many studies regarding regulation of c-fos are conducted in vivo using rats, or with rat cell lines, we have cloned the rat c-fos gene in order to verify that the same regulatory elements that have been characterized in the human and mouse c-fos promoter are also present and functional in the rat. The nucleotide (nt) sequence of the 5'-flanking region of the rat c-fos gene displays remarkable similarity with the mouse and human c-fos genes (93 and 77% identity, respectively). cis-Acting regulatory elements, such as the sis-inducible element (SIE), serum-response element (SRE), AP-1-recognition site, calcium/cAMP-response element (Ca/CRE) and TATA box are present in the rat c-fos 5'-flanking region. A putative glucocorticoid-response element (GRE) is present 13 bp downstream from the AP-1-recognition site.
Wang
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WW
Department of Biochemistry and Molecular Biology, UMD-New Jersey Medical School, Newark 07103.
Howells
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RD
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016762:Genes, fos; D015698:Genomic Library; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D051381:Rats; D017207:Rats, Sprague-Dawley; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid