Cloning genes from an inner ear cDNA library.
A rat inner ear complementary DNA (cDNA) library containing 1.9 x 10(6) recombinants was constructed and evaluated. Inserts averaged 2.0 (+/- 2.1) kilobases in length. A subset of inserts was screened for site of expression. Two cDNA transcripts were isolated based on cochlear expression restricted to the spiral ganglion. One transcript showed a high degree of homology to several long interspersed DNA elements, neuron-specific nuclear transcripts thought to be involved in gene regulation. The second transcript showed no homology to known sequences and appears to encode a neuron-specific protein of about 248 amino acids. The library can be used to identify proteins important for inner ear function and disease.
Ryan
A F
AF
Department of Surgery, University of California-San Diego School of Medicine, La Jolla.
Batcher
S
S
Brumm
D
D
O'Driscoll
K
K
Harris
J P
JP
D000818:Animals; D003001:Cloning, Molecular; D003051:Cochlea; D018076:DNA, Complementary / Q000235:genetics*; D007758:Ear, Inner; D005784:Gene Amplification; D015723:Gene Library; D051381:Rats
D018076:DNA, Complementary