Detection of human papilloma virus (HPV) infection in paraffin-embedded tissues of endometrial carcinoma.
Although human papilloma virus (HPV) associated lesions constitute a well recognized clinical entity in the female lower genital tract, namely vulva, vagina and cervix, few studies have demonstrated HPV infection in other genital sites, particularly in the ovary and uterine corpus. Recently, with the highly sensitive polymerase chain reaction (PCR) technique, HPV infections were found in an ovarian tumour and adenocarcinoma of the cervix. This prompted a retrospective analysis of HPV DNA in 22 cases of endometrial adenocarcinoma in order to investigate the possible carcinogenesis of HPV in the uterine corpus. In this study DNA extraction was performed from paraffinized cancerous tissues and the normal cervical counterpart. HPV 6, 11, 16 and 18 primers specific oligonucleotides were used in PCR to detect the presence of this oncogenic virus. HPV 16 DNA was found in 1 endometrial adenocarcinoma and 4 cervical tissues. Our result did not support the aetiological role of HPV in the carcinogenesis of endometrial carcinoma.
Wong
W S
WS
Department of Obstetrics and Gynaecology, Chinese University of Hong Kong, Prince of Wales Hospital.
Wong
Y F
YF
Tam
O S
OS
Tam
J S
JS
D000230:Adenocarcinoma / Q000235:genetics / Q000382:microbiology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D016889:Endometrial Neoplasms / Q000235:genetics / Q000382:microbiology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016612:Paraffin Embedding; D012189:Retrospective Studies; D014412:Tumor Virus Infections / Q000175:diagnosis*
D017931:DNA Primers; D004279:DNA, Viral