Entamoeba histolytica: electron-dense granule secretion, collagenase activity and virulence are altered in the cytoskeleton mutant BG-3.
HM-1:IMSS, a pathogenic strain of Entamoeba histolytica, and its mutant BG-3, identified by resistance to cytochalasin D, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type I collagen gels; and (iii) produce liver abscesses in new-born hamsters. The results demonstrate that the mutant has low adherence to collagen, low electron-dense granule secretion and collagenolytic activity, and low capacity to produce liver lesions in vivo, compared with the parental strain HM1:IMSS.
Serrano
J J
JJ
Department of Cell Biology, Centro de Investigación y de Estudios Avanzados del I.P.N., México, D.F.
de la Garza
M
M
Moreno
M A
MA
Tovar
R
R
León
G
G
Tsutsumi
V
V
Muñoz
M L
ML
D000818:Animals; D000831:Animals, Newborn; D002448:Cell Adhesion; D003094:Collagen; D017364:Collagenases / Q000032:analysis*; D006224:Cricetinae; D015638:Cytochalasin D / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000378:metabolism*; D003599:Cytoskeleton / Q000648:ultrastructure*; D004351:Drug Resistance / Q000235:genetics; D004748:Entamoeba histolytica / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity / Q000648:ultrastructure; D008101:Liver Abscess, Amebic / Q000469:parasitology; D008647:Mesocricetus; D009154:Mutation; D015800:Protozoan Proteins / Q000032:analysis*; D014774:Virulence / Q000235:genetics
D015800:Protozoan Proteins; D015638:Cytochalasin D; D003094:Collagen; D017364:Collagenases