title: The cleavage of phosphoenolpyruvate by vanadate.
abstract: Vanadate rapidly promotes the cleavage of phosphoenolpyruvate with phosphate liberation. This was not observed when ATP, glucose-6-phosphate and acetyl phosphate were incubated with vanadate. 51V NMR spectra shows that phosphoenolpyruvate and acetyl phosphate broadened and shifted upfield the monomeric vanadate signal at -561 ppm, indicative of vanadate/phosphate interactions. Comparatively, smaller changes were detected when glucose-6-phosphate was added to the vanadate solution. The shift behavior was not observed in the presence of ATP, ADP or pyruvate.
authors.affiliation: Departamento de Zoologia, Universidade de Coimbra, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Aureliano
authors.forename: J
authors.initials: J
authors.lastname: Leta
authors.forename: V M
authors.initials: VM
authors.lastname: Madeira
authors.forename: L
authors.initials: L
authors.lastname: de Meis
mesh_terms: D000227:Adenine Nucleotides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010728:Phosphoenolpyruvate / Q000737:chemistry*; D014638:Vanadates / Q000737:chemistry*
chemical_list: D000227:Adenine Nucleotides; D014638:Vanadates; D010728:Phosphoenolpyruvate