Prenatal exclusion of paternity by PCR-FLP analysis of VNTR.
A prenatal paternity test was requested by a 30-year-old woman. Variable number of tandem repeat (VNTR) systems were used for DNA analysis by means of amplification and electrophoresis followed by ethidium bromide staining or Southern blotting and oligonucleotide hybridization. Exclusion of paternity could be established on the basis of the great polymorphism and heterozygosity indexes of these genetic systems. This rapid method presents several advantages in contrast with other recombinant DNA techniques such as HLA class II oligotyping or RFLP.
Arroyo
E
E
Laboratoire de Histocompatibilidad, Centro de Transfusión, de la Communidad de Madrid, Spain.
García-Sánchez
F
F
Gómez-Reino
F
F
Ruiz de la Cuesta
J M
JM
Vicario
J L
JL
D000328:Adult; D001345:Autoradiography; D001483:Base Sequence; D015193:Chorionic Villi Sampling; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006580:Genetic Carrier Screening; D006801:Humans; D008969:Molecular Sequence Data; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA