Studies on the possible biological effects of 50 Hz electric and/or magnetic fields: evaluation of some glycolytic enzymes, glycolytic flux, energy and oxido-reductive potentials in human erythrocytes exposed in vitro to power frequency fields.
An attempt has been made to understand whether 50 Hz electric and magnetic fields (EMFs) are involved in producing bioeffects by exposing human erythrocytes in vitro. The study evaluated some key glycolytic enzymes, glucose consumption, lactate production, energy charge, 2,3-diphosphoglycerate, and reduced glutathione levels, all of which are biochemical parameters significant to erythrocyte function. Cells exposed to individual or superimposed EMFs have not shown any significant difference compared with the controls.
Dachà
M
M
Centro di Metodologie Biochimiche Applicate, Università degli Studi di Urbino, Italy.
Accorsi
A
A
Pierotti
C
C
Vetrano
F
F
Mantovani
R
R
Guidi
G
G
Conti
R
R
Nicolini
P
P
D019794:2,3-Diphosphoglycerate; D000227:Adenine Nucleotides / Q000097:blood; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000097:blood; D004574:Electromagnetic Fields / Q000009:adverse effects*; D004734:Energy Metabolism / Q000528:radiation effects; D004912:Erythrocytes / Q000378:metabolism / Q000528:radiation effects*; D005978:Glutathione / Q000097:blood; D006019:Glycolysis / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008875:Middle Aged; D010084:Oxidation-Reduction
D000227:Adenine Nucleotides; D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D007773:Lactates; D019794:2,3-Diphosphoglycerate; D019344:Lactic Acid; D005978:Glutathione