Density change of neutrophils from allergic subjects by granulocyte-macrophage colony stimulating factor.
We investigated neutrophil density change by platelet activating factor or granulocyte-macrophage colony-stimulating factor (GM-CSF). Although PAF significantly converted neutrophil density, there was no significant difference in density change between allergic subjects and normal subjects by platelet activating factor. Neutrophils from allergic subjects, however, were significantly converted by GM-CSF when compared with neutrophils from normals (P < .05). We conclude that neutrophils from allergic subjects are more sensitive to density change by GM-CSF than neutrophils from normal subjects. This might be due to preactivation or priming by biologic agents.
Miyagawa
H
H
Allergic Disease Center, Creighton University School of Medicine, Omaha, Nebraska.
Okada
C
C
Sugiyama
H
H
Hopp
R J
RJ
Bewtra
A K
AK
Townley
R G
RG
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood*; D007958:Leukocyte Count / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology*; D010972:Platelet Activating Factor / Q000494:pharmacology
D010972:Platelet Activating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor