A highly sensitive immunoenzymometric assay for the determination of angiogenin.
A polyclonal antibody to human recombinant angiogenin was prepared in rabbits using a Pam3CysSerGly conjugate. The antibody was then used to develop the first highly sensitive enzyme-labelled immunometric assay for this vascularisation inducing and tumour associated protein. The assay was suitable for quantification of angiogenin in body fluids between 2.5 and 0.05 micrograms/l. The mean intra-assay imprecision was 6.0% and the inter-assay imprecision 7.9%. Angiogenin in human plasma was found to lie in the range of 0.38 to 0.11 mg/l with a mean of 0.25 +/- 0.07 mg/l.
Bläser
J
J
Department of Biochemistry, University of Bielefeld, Germany.
Triebel
S
S
Kopp
C
C
Tschesche
H
H
D043925:Angiogenesis Inducing Agents / Q000032:analysis*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D011994:Recombinant Proteins / Q000032:analysis; D015203:Reproducibility of Results; D012259:Ribonuclease, Pancreatic; D012680:Sensitivity and Specificity
D043925:Angiogenesis Inducing Agents; D009363:Neoplasm Proteins; D011506:Proteins; D011994:Recombinant Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic