title: Site-specific DNA cleavage by Cu(II) complexes of podophyllotoxin derivatives.
abstract: Site-specific DNA cleavage in the presence of Cu(II) complexes of podophyllotoxin derivatives was investigated with a modified Sanger sequencing method. Cu(II) complexes of 4'-demethylepipodophyllotoxin (DEPD) and syringic acid (SA) cleaved M13mp18 single-strand DNA site-specifically at both cytosine (C) and guanine (G) positions in the GC rich regions and C position, respectively, at pH 7.8. The apparent binding constants of calf thymus DNA-Cu(II) complexes estimated by the differential UV-absorption spectra revealed that both Cu(II)-VP-16 and -DEPD complexes bind to DNA more strongly than does the Cu(II)-SA complex.
authors.affiliation: Department of Analytical Chemistry, Kyoto Pharmaceutical University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yamashita
authors.forename: R
authors.initials: R
authors.lastname: Tawa
authors.forename: Y
authors.initials: Y
authors.lastname: Imakura
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Sakurai
mesh_terms: D001483:Base Sequence; D003300:Copper / Q000494:pharmacology*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects / Q000235:genetics; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011034:Podophyllotoxin / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004277:DNA, Single-Stranded; D005707:Gallic Acid; D003300:Copper; C001945:syringic acid; D004259:DNA-Directed DNA Polymerase; D011034:Podophyllotoxin