[Establishment of a cisplatin-resistant human ovarian cancer cell line].
A human ovarian cancer cell line designated "MH" was established from ascites of a patient with ovarian serous cystadenocarcinoma treated with cisplatin. This cell line was grown for more than 2 years and over 100 passages in medium RPMI1640 containing 10% FCS. The cell doubling time, saturation density and plating efficiency was approximately 6.3 days, 5.5 x 10(4)/cm2, and 42%, respectively. Chromosomal analysis revealed aneuploidy with a modal number of 72 and 14-19 types of marker chromosomes. CA125 was detected in both the original tumor and the cultured cells. This cell line is cisplatin-resistant (IC50: 3.28 microM) and has high protein kinase C activity.
Sasa
H
H
Department of Obstetrics and Gynecology, National Defense Medical College, Tokorozawa, Japan.
Hisano
A
A
Ishii
K
K
Hirata
J
J
Kikuchi
Y
Y
Nagata
I
I
Senoo
A
A
Aida
S
S
Tamai
S
S
Sugita
M
M
D000328:Adult; D000782:Aneuploidy; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002945:Cisplatin / Q000494:pharmacology*; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000473:pathology*; D004351:Drug Resistance; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002945:Cisplatin