Increased apolipoprotein-E concentrations in individuals suffering chronic low back syndrome identified by two-dimensional gel electrophoresis.
Non-biased screening of plasma proteins by two-dimensional gel electrophoresis from individuals suffering low back syndrome revealed a polypeptide spot that was increased 2-5-fold over the concentration found in normal control individuals. The apparent molecular weight (34-36 kDa) and pI (5.7) of this spot suggested that it might be apolipoprotein-E. Immunoblot analysis showed that the polypeptide was reactive with anti-apolipoprotein-E antibodies. N-terminal amino acid microsequence confirmed the identify of this polypeptide as apolipoprotein-E. We have determined that elevated plasma levels of apolipoprotein-E is associated with inflammation and nerve damage.
VanderPutten
D M
DM
Monoclonetics International, Inc, Houston, TX.
Cameron
B M
BM
Merril
C R
CR
D000595:Amino Acid Sequence; D001057:Apolipoproteins E / Q000378:metabolism*; D001696:Biomechanical Phenomena; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D017116:Low Back Pain / Q000097:blood*; D008969:Molecular Sequence Data; D010146:Pain / Q000097:blood
D001057:Apolipoproteins E