PCR cloning and sequence of the murine GPIIb gene promoter.
The promoter region of the murine GPIIb gene was cloned by PCR using oligonucleotides corresponding to the human gene promoter. Analysis of the sequence revealed homologies among species and the conservation of DNA motifs which could be implicated in the regulation of the gene. Transfection experiments show that the murine promoter contains informations for a tissue specific expression of the gene in megakaryocytic cell lines.
Denarier
E
E
Laboratoire d'hématologie, INSERM U217, Grenoble, France.
Martin
F
F
Martineau
S
S
Marguerie
G
G
D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D010980:Platelet Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase