Cloning of cDNA for the beta-subunit of rabbit translation initiation factor-2 using PCR.
RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. Rabbit eIF-2 beta transcripts were then specifically amplified by PCR and sequenced. RACE (rapid amplification of cDNA ends) was used to obtain 3' and 5' sequences. Comparison of the deduced amino acid sequence with that of human eIF-2 beta reveals a very high degree of sequence identity.
Price
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NT
Department of Biochemistry, University of Bristol, UK.
Hall
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L
Proud
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CG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis*; D015852:Eukaryotic Initiation Factor-2 / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits
D018076:DNA, Complementary; D015852:Eukaryotic Initiation Factor-2