Immunization of mice by injection with a recombinant retrovirus vector containing human factor IX for production of monoclonal antibody against factor IX.
A novel immunization procedure for eliciting murine monoclonal antibodies (mAb) is described. A murine leukemia virus (MLV)-based retroviral vector, designed as a marker protein for the expression of human factor IX (FIX), was constructed. Direct injections of mice with 0.8-1.3 x 10(4) retroviruses were carried out three times at 4-week intervals. Three out of 4 mice that had the viruses injected subcutaneously produced the antibodies against FIX, and 2 out of 3 mice injected intraperitoneally produced the antibodies. From 1 of the mice with the antibodies, an anti-human FIX murine monoclonal antibody, designated ACTIX (IgMk type), was produced. The immunization of mice by direct injection of viruses facilitated mAb production in many instances in which cDNAs are available.
Takahashi
I
I
Japanese Red Cross Aichi Blood Center, Aichi.
Mizuno
S
S
Uchigiri
C
C
Emi
N
N
Kamiya
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D005164:Factor IX / Q000276:immunology*; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D008979:Moloney murine leukemia virus; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D005164:Factor IX