Identification of buccal mucosal mucin receptor.
A receptor for salivary mucin was isolated from epithelial cell membrane of buccal mucosa by a procedure involving membrane solubilization with octylglucoside followed by affinity chromatography on Sepharose-bound wheat germ agglutinin. The receptor protein displayed an apparent molecular weight of 97kDa and exhibited binding specific to mucin in a concentration-dependent manner. The receptor, furthermore, showed a requirement for carbohydrate chains in mucin for binding, as their removal caused a marked (89%) reduction in binding capacity. Scatchard analysis revealed a linear plot with a single class of high affinity binding (Kd = 1.1 microM; Bmax = 7.68nmol/mg protein. The results provide for the first time evidence for the existence in buccal mucosa of a specific receptor for salivary mucin.
Slomiany
B L
BL
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Piotrowski
J
J
Majka
J
J
Liau
Y H
YH
Slomiany
A
A
D000818:Animals; D050260:Carbohydrate Metabolism; D002610:Cheek; D002846:Chromatography, Affinity; D066298:In Vitro Techniques; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism*; D009077:Mucins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013363:Submandibular Gland / Q000378:metabolism
D009077:Mucins; D011956:Receptors, Cell Surface; C085661:mucin receptor