title: Characterization of a chicken cDNA encoding the retinoblastoma gene product.
abstract: We have isolated a chicken cDNA that encodes the retinoblastoma susceptibility gene product (RB). The predicted amino acid sequence of the chicken RB protein is highly similar to that of the mouse, human and Xenopus RB proteins in regions of known functions; however, chicken RB has distinct species-specific differences, including a shorter N-terminal region as compared to the mouse and human RB proteins. In vitro-translated chicken RB co-migrates on SDS-polyacrylamide gels with endogenous RB synthesized in transformed chicken spleen cells. Finally, chicken RB is located in the nucleus of chicken embryo fibroblasts when overexpressed from a retroviral vector.
authors.affiliation: Department of Biology, Boston University, MA 02215-2406.
authors.forename: R
authors.initials: R
authors.lastname: Feinstein
authors.forename: W K
authors.initials: WK
authors.lastname: Bolton
authors.forename: J N
authors.initials: JN
authors.lastname: Quinones
authors.forename: G
authors.initials: G
authors.lastname: Mosialos
authors.forename: S
authors.initials: S
authors.lastname: Sif
authors.forename: J L
authors.initials: JL
authors.lastname: Huff
authors.forename: A J
authors.initials: AJ
authors.lastname: Capobianco
authors.forename: T D
authors.initials: TD
authors.lastname: Gilmore
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens; D004247:DNA; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D016160:Retinoblastoma Protein / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D016160:Retinoblastoma Protein; D004247:DNA