Role of hemocyte-derived granular components in invertebrate defense.
Figure 2 illustrates an outline of the cellular and humoral defense systems in limulus. On the basis of the knowledge described above, it is suggested that granular components present in L and S granules in the hemocytes play a decisive role in the biological defense for this animal. The isolated L granules contain at least three clotting factors plus coagulogen as the major component. The known anti-LPS factor and a number of additional unknown protein components are also present in the L granules. On the other hand, the isolated S granules contain antimicrobial tachyplesins as the major component, in addition to six unidentified proteins. We speculate that the L-granule-derived protein components, which probably contain all the factors essential for the Limulus clotting system participate, in immobilizing invading microbes, and that the S-granule-derived tachyplesins contribute to a self-defense system against invaders. Although we have not mentioned hemolymph plasma components, there are many humoral factors, such as proteinase inhibitors, alpha 2-macroglobulin, various lectins, C-reactive protein, and polyphemin, all of which are important for antimicrobial defense. Furthermore, Liu and colleagues have reported several endotoxin-binding proteins and a cell-adhesion protein found in the Limulus hemocytes. Although the exact functions of these substances are unknown, they may act in concert with other components to provide biological defense for the animal. Nevertheless, compared to our knowledge of mammalian blood cells, much less remains to be learned of biological/physiological events in horseshoe crab hemocytes.
Iwanaga
S
S
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Muta
T
T
Shigenaga
T
T
Miura
Y
Y
Seki
N
N
Saito
T
T
Kawabata
S
S
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D001779:Blood Coagulation Factors / Q000235:genetics / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism; D006434:Hemocytes / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D006458:Hemolymph / Q000276:immunology; D006737:Horseshoe Crabs / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000235:genetics / Q000276:immunology; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D001779:Blood Coagulation Factors; D004268:DNA-Binding Proteins; D004792:Enzyme Precursors; D010456:Peptides, Cyclic; C057636:tachyplesin peptide, Tachypleus tridentatus; D010450:Endopeptidases; C085383:coagulation factor G, horseshoe crab; D012697:Serine Endopeptidases; C057151:Limulus clotting factor C; C036776:Limulus clotting factor B; C014775:pro-clotting enzyme