title: [Alloimmune neonatal neutropenia: flow cytometry study of the 1st patient described in Spain with identification of an anti-NA1].
abstract: A strong antibody was found in a mother (first pregnancy) who had a severe neutropenic baby. The father's granulocytes were typed by flow cytometry as NA1+, NA2+, NB1+, ND1+ and the mother as NA1-, NA2+, NB1+ and ND1+. The antibody was identified as anti-NA1 by us, and confirmed later by a reference laboratory. The serum reacted with 54.8% of the 31 donors tested. The same antibody was found in the child's serum 35 days after birth, and the reactivity was stronger than in the mother's serum. The HLA-DR, DQ from the mother was DR3, DR7; DR52, DR53; DQ2. The baby's granulocytes were recovered slowly over a four-month period, but the course was benign without any specific treatment. Five months after birth, with recovery, the child's serum became negative and his granulocytes were confirmed as NA1+. Due to the difficulties in fully diagnosing and working with granulocytes we suspect that there are undetected cases; only one case has been recorded in Spain before.
authors.affiliation: Sección de inmunologíca leucoplaquetar, Centro de Transfusión de la Comunidad Valenciana.
authors.forename: N
authors.initials: N
authors.lastname: Puig
authors.forename: J A
authors.initials: JA
authors.lastname: Montoro
authors.forename: J L
authors.initials: JL
authors.lastname: Villalba
authors.forename: M
authors.initials: M
authors.lastname: Gimeno
authors.forename: I
authors.initials: I
authors.lastname: Ample
authors.forename: J
authors.initials: J
authors.lastname: Molina
authors.forename: M
authors.initials: M
authors.lastname: Sagaseta
authors.forename: A
authors.initials: A
authors.lastname: Asensio
mesh_terms: D005260:Female; D006681:HLA-D Antigens / Q000235:genetics; D006801:Humans; D007112:Immunity, Maternally-Acquired; D007231:Infant, Newborn; D007518:Isoantibodies / Q000276:immunology*; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D008297:Male; D009503:Neutropenia / Q000151:congenital* / Q000209:etiology / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D011961:Receptors, Fc / Q000235:genetics / Q000276:immunology*; D013030:Spain
chemical_list: D006681:HLA-D Antigens; D007518:Isoantibodies; D007519:Isoantigens; D011961:Receptors, Fc; C067704:neutrophil-specific antigen NA1, human
vernacular_title: Neutropenia neonatal aloinmune: estudio por citometría de flujo del primer caso descrito en España con identificación de un anti-NA1.