title: Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis.
abstract: A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 micrograms/g) into female lampreys undergoing final maturation before spawning produced a significant (P < 0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.
authors.affiliation: Department of Biomedical Sciences, Creighton University Medical School, Omaha, NE 68178.
authors.forename: J M
authors.initials: JM
authors.lastname: Conlon
authors.forename: A
authors.initials: A
authors.lastname: Balasubramaniam
authors.forename: S A
authors.initials: SA
authors.lastname: Sower
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D007798:Lampreys / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D011863:Radioimmunoassay
chemical_list: D009478:Neuropeptide Y; D004958:Estradiol