Correspondence between a mammalian spliceosome component and an essential yeast splicing factor.
None of the mammalian splicing factors that have been cloned corresponds to the yeast pre-messenger RNA splicing factors, the PRP proteins. Here, a generalizable strategy was used to isolate a complementary DNA encoding the mammalian spliceosome-associated protein (SAP) SAP 62. It is demonstrated that SAP 62 is the likely functional homolog of the yeast PRP11 protein. Both PRP11 and SAP 62 associate stably with the spliceosome, contain a single zinc finger, and display significant amino acid sequence similarity. Unlike PRP11, SAP 62 contains 22 proline-rich heptapeptide repeats at the carboxyl-terminus.
Bennett
M
M
Department of Cell Biology, Harvard Medical School, Boston, MA 02115.
Reed
R
R
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000737:chemistry* / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; D012091:Repetitive Sequences, Nucleic Acid; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017381:Spliceosomes / Q000737:chemistry* / Q000378:metabolism; D016335:Zinc Fingers
D002352:Carrier Proteins; D005656:Fungal Proteins; D009698:Nucleoproteins; C068835:PRP11 protein, S cerevisiae; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; C083433:SF3A2 protein, human; D029701:Saccharomyces cerevisiae Proteins