Immunoelectron microscopic distribution of actin in the spermatids and epididymal spermatozoa of the mouse.
The distribution of actin in spermatids and epididymal spermatozoa of the mouse was examined by electron microscopy with immunogold procedures using two monoclonal antiactin antibodies. Actin was identified by immunoblotting of proteins extracted from either whole spermatozoa or tail fractions. In round spermatids, actin immunolabeling was detected in the subacrosomal space. Labeling increased during the elongation phase, and then decreased and completely disappeared before spermiation. No labeling was found in epididymal spermatozoa and residual bodies. Our results suggest actin redistribution or the absence of G-actin from the head to the flagellum in mature murine spermatids, as previously shown in other studies in various mammals.
Prigent
Y
Y
Laboratoire d'Histologie, UER Biomédicale, Paris, France.
Dadoune
J P
JP
D000199:Actins / Q000032:analysis*; D000818:Animals; D004822:Epididymis / Q000166:cytology*; D008297:Male; D051379:Mice; D016253:Microscopy, Immunoelectron; D013087:Spermatids / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry
D000199:Actins