Expression of secreted immunoglobulin heavy chain genes and immunoglobulin-secreting cells in human lymphocytes.
The numbers of immunoglobulin-secreting cells in peripheral blood mononuclear cells and the expression of mRNA for secreted type of immunoglobulin heavy chains were investigated in healthy children, compared with the percentages of surface immunoglobulin-bearing cells and the expression of mRNA for membrane-bound type of immunoglobulin heavy chains, respectively. Although a difference between expression of mu s mRNA and mu m mRNA was unclear, mu mRNA was well transcribed. The expression of gamma s mRNA or alpha s mRNA was markedly higher than that of gamma m mRNA or alpha m mRNA. However, although the detection methods could be of different sensitivities, the percentage of IgM-, IgG-, or IgA-secreting cells was markedly low, compared with the percentage of surface IgM-, IgG-, or IgA-bearing cells, respectively. Therefore, additional regulation of the pattern of the immunoglobulin gene expression may be exerted at the translational and post-translational stages.
Kondo
N
N
Department of Pediatrics, Gifu University School of Medicine, Japan.
Inoue
R
R
Agata
H
H
Suzuki
Y
Y
Shimozawa
N
N
Tomatsu
S
S
Nakashima
Y
Y
Yamagishi
A
A
Ogawa
T
T
Orii
T
T
D000921:Antibody-Producing Cells / Q000378:metabolism*; D015152:Blotting, Northern; D002648:Child; D015342:DNA Probes; D015870:Gene Expression; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics; D016130:Immunophenotyping; D008214:Lymphocytes / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D011947:Receptors, Antigen, B-Cell / Q000276:immunology
D015342:DNA Probes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D012333:RNA, Messenger; D011947:Receptors, Antigen, B-Cell