Bacitracin production by a new strain of Bacillus subtilis. Extraction, purification, and characterization.
A new strain of Bacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth of Micrococcus flavus. The production of the antibiotic in culture medium followed to extraction with n-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.
Azevedo
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EC
Mestrado de Ciencias Farmacêuticas, Universidade Federal de Pernambuco, Recife-PE, Brazil.
Rios
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EM
Fukushima
K
K
Campos-Takaki
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GM
D020001:1-Butanol; D001412:Bacillus subtilis / Q000378:metabolism*; D001414:Bacitracin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000440:Butanols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003470:Culture Media; D008826:Microbial Sensitivity Tests; D008837:Micrococcus / Q000187:drug effects* / Q000254:growth & development
D000440:Butanols; D003470:Culture Media; D001414:Bacitracin; D020001:1-Butanol