Block in porcine gamete interaction by polyclonal antibodies to a pig ZP3 beta fragment having partial sequence homology to human ZP3.
A murine monoclonal antibody (MA-30) recognizing a sequential epitope on porcine zona pellucida-3 beta glycoprotein (ZP3 beta) and capable of inhibiting sperm-egg interaction was previously described. Polyclonal antibodies against a approximately 6 kDa fragment from the tryptic digest of ZP3 beta, reacting with MA-30, can also inhibit porcine gamete interaction in vitro. Partial N-terminal sequencing of the smallest fragment from the ZP3 beta tryptic digest having reactivity with MA-30 shows sequence homology with human ZP3.
Bagavant
H
H
Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, Delhi, India.
Yurewicz
E C
EC
Sacco
A G
AG
Talwar
G P
GP
Gupta
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001667:Binding, Competitive; D004527:Egg Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011956:Receptors, Cell Surface; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013084:Sperm-Ovum Interactions / Q000276:immunology*; D013552:Swine; D000072216:Zona Pellucida Glycoproteins
D000906:Antibodies; D004527:Egg Proteins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; C000606517:ZP3 protein, human; D000072216:Zona Pellucida Glycoproteins; C000606521:Zp3 protein, mouse