Calponin phosphorylation does not accompany contraction of various smooth muscles.
Calponin phosphorylation was quantitated in 32P-labeled porcine arterial, uterine, tracheal, stomach and bladder smooth muscles. The negligible amount of 0.003-0.008 mol [32P]phosphate/mol calponin in resting muscles did not increase upon contraction induced by various agents, under conditions when myosin light-chain phosphorylation increased several-fold. The results indicate no involvement of calponin phosphorylation is smooth-muscle contraction.
Bárány
M
M
Department of Biochemistry, College of Medicine, University of Illinois at Chicago 60612.
Bárány
K
K
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D008840:Microfilament Proteins; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011487:Protein Conformation; D013552:Swine; D014335:Tropomyosin / Q000378:metabolism; D000096985:Calponins
D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D009218:Myosins; D010761:Phosphorus Radioisotopes; D014335:Tropomyosin; D000096985:Calponins