title: Studies into the mechanism for MHV transcription.
abstract: Previous studies have demonstrated that the MHV genome is divided into seven transcriptional units which are transcribed from highly conserved intergenic start sites (UCU/CAAAC) into mRNA containing a common leader RNA at the 5' end and a coterminal 3' end. In this manuscript, we provide evidence that an additional transcriptional unit is encoded at the 3' end of the MHV genome and is transcribed from a perfect intergenic region into a leader-containing approximately 800 nt mRNA. This mRNA could potentially encode a small 17-18 kDa protein which is identical to the C-terminal third of the nucleocapsid gene.
authors.affiliation: Department of Epidemiology, University of North Carolina at Chapel Hill 27599-7400.
authors.forename: M C
authors.initials: MC
authors.lastname: Schaad
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: S A
authors.initials: SA
authors.lastname: Peel
authors.forename: R S
authors.initials: RS
authors.lastname: Baric
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics; D016384:Consensus Sequence; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014758:Viral Core Proteins; D015678:Viral Structural Proteins