title: Interleukin-6 production by human hepatoma lines is related to a low degree of cell differentiation.
abstract: In this study, we showed by Northern blot analysis and bioassays that scarcely differentiated human hepatoma cell line HA22T/VGH constitutively produces interleukin-6 (IL6). This cytokine was produced neither by moderately differentiated Li7A nor by well-differentiated HepG2 human hepatoma cell lines. The finding that transcripts for IL6 were not present in 2.2.15 cells, a HepG2-derived clone transfected with the intracellular replicative form of hepatitis B virus (HBV) DNA, suggests that production of this cytokine is not affected by HBV-encoded gene products, but is more likely related to the dedifferentiated state of hepatoma cells.
authors.affiliation: Fondazione Andrea Cesalpino, I Clinica Medica, Università La Sapienza, Roma, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Paroli
authors.forename: G
authors.initials: G
authors.lastname: Carloni
authors.forename: E
authors.initials: E
authors.lastname: Alfani
authors.forename: G
authors.initials: G
authors.lastname: De Petrillo
authors.forename: V
authors.initials: V
authors.lastname: Barnaba
mesh_terms: D001681:Biological Assay; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008113:Liver Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger