title: A perfusion chamber with temperature regulation.
abstract: When investigating microscopic preparations perfusion chambers allow exchange and regulation of different solutions and ensure their constant flow in the sample chamber. Temperature deviations, however, may be problematic. We describe a new chamber that contains an additional circulation system which regulates the inside temperature using an external thermostat. An integrated thermometer probe records the sample temperature, which appears on a monitor. The glass chamber, measuring 75 x 35 x 3 mm, provides good optical quality and is compatible with every type of microscope.
authors.affiliation: Institute of Plant Physiology, Graz, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Sevcik
authors.forename: H
authors.initials: H
authors.lastname: Guttenberger
authors.forename: D
authors.initials: D
authors.lastname: Grill
mesh_terms: D008853:Microscopy; D010477:Perfusion / Q000295:instrumentation*; D011312:Pressure; D013696:Temperature