title: Potential use of BacT/Alert automated blood culture system for antifungal susceptibility testing.
abstract: A simple, rapid susceptibility test is needed to determine the possible resistance of yeasts to commonly used antifungal agents. The BacT/Alert automated blood culture system was evaluated as one technology for development of such a test. Yeast nitrogen base was used as the growth medium, and amphotericin B was the test antifungal agent. Isolates of various Candida species, Torulopsis glabrata, Saccharomyces cerevisiae, and Cryptococcus neoformans were evaluated. The results suggest that detection of amphotericin B resistance of yeast isolates within 12 to 14 h after inoculation of test medium is possible.
authors.affiliation: Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908.
authors.forename: K C
authors.initials: KC
authors.lastname: Hazen
authors.forename: M P
authors.initials: MP
authors.lastname: Chery
authors.forename: Y
authors.initials: Y
authors.lastname: Han
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology; D003470:Culture Media; D005069:Evaluation Studies as Topic; D016469:Fungemia / Q000188:drug therapy / Q000382:microbiology; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009181:Mycoses / Q000188:drug therapy / Q000382:microbiology; D012441:Saccharomyces cerevisiae / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D000666:Amphotericin B