title: Soaking nitrocellulose blots in acidic buffers improves the detection of bound antibodies without loss of biological activity.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: W L
authors.initials: WL
authors.lastname: Hoffman
authors.forename: A A
authors.initials: AA
authors.lastname: Jump
authors.forename: A O
authors.initials: AO
authors.lastname: Ruggles
mesh_terms: D000143:Acids; D000906:Antibodies / Q000032:analysis*; D002021:Buffers; D003101:Collodion; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing; D007700:Kinetics; D008567:Membranes, Artificial; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D012685:Sepharose
chemical_list: D000143:Acids; D000906:Antibodies; D002021:Buffers; D007457:Iodine Radioisotopes; D008567:Membranes, Artificial; D011506:Proteins; D003101:Collodion; D012685:Sepharose