[Reaction of the complement fixation test on microtitration plates: application to the serology of myxomatosis. Comparative study of the results with the reaction of indirect immunofluorescence].
The authors describe a complement fixation technique on microtitration plates, using an antigen prepared from myxomas induced in rabbits. Compared with indirect immunofluorescence this technique was less cumbersome, more economical, easier to read and (as a conventional procedure) applicable in all laboratories. Results obtained with 165 serum samples tested by both methods showed good correlation and a specificity at least equal to that of indirect immunofluorescence. Taking into account its lower sensitivity, the positive threshold value for complement fixation under the described experimental conditions was a dilution of 1:4 (H50).
Chantal
J
J
Département de biologie moléculaire, Ecole nationale vétérinaire, Toulouse, France.
Boucraut-Baralon
C
C
Ganière
J P
JP
Petit
F
F
Py
R
R
Picavet
D P
DP
D000818:Animals; D003168:Complement Fixation Tests / Q000662:veterinary*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D009234:Myxomatosis, Infectious / Q000175:diagnosis*; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
Réaction de fixation du complément en plaques de microtitration: application à la sérologie de la myxomatose. Etude comparative des résultats avec la réaction d'immunofluorescence indirecte.