A preliminary translational map of the frog virus 3 genome.
A preliminary map of the frog virus 3 (FV 3) genome was constructed by hybridization-selection of mRNAs to cloned DNA fragments and translation in reticulocyte lysates. FV 3 mRNAs were hybridized to KpnI, HindIII, and SalI restriction fragments representing the entire FV 3 genome. Two different hybridization conditions were employed in order to discriminate between the hybridization of early and late mRNAs. A total of 43 major and 18 minor early genes and nine major and three minor late genes were mapped. A 24 kb region comprised of the KpnI D, H, and E fragments encodes 8 of the 12 late genes that were mapped.
Foglesong
P D
PD
Division of Virology and Molecular Biology, St. Jude Children's Research Center, Memphis, TN.
Willis
D B
DB
D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D017995:Ranavirus / Q000235:genetics*; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins