In vivo administration of a monoclonal antibody against the type I IL-1 receptor inhibits the ability of mice to eliminate Mycobacterium paratuberculosis.
The purpose of this study was to determine the influence of endogenous interleukin-1 (IL-1) on resistance to paratuberculosis infection in experimentally infected gnotobiotic mice. Following a 6-month treatment with prednisolone to facilitate bacillary multiplication, control mice substantially reduced the numbers of M. paratuberculosis in the liver and ileum. In contrast, mice injected with a monoclonal antibody against the type I IL-1 receptor failed to reduce the numbers of M. paratuberculosis in the liver and ileum and exhibited more liver granulomas, which contained numerous acid-fast bacilli. These results indicate a significant role for endogenous IL-1 in host defense against experimental M. paratuberculosis infection in mice.
Kenefick
K B
KB
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison.
Adams
J L
JL
Steinberg
H
H
Czuprynski
C J
CJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D006099:Granuloma / Q000503:physiopathology / Q000517:prevention & control; D007082:Ileum / Q000382:microbiology*; D008099:Liver / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016927:Mycobacterium avium subsp. paratuberculosis / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000276:immunology* / Q000517:prevention & control; D011239:Prednisolone / Q000494:pharmacology; D017472:Receptors, Interleukin-1 / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017472:Receptors, Interleukin-1; D011239:Prednisolone