On the use of 2,4,5-trihydroxyphenethylamine as a rapid method for laccase quantification.
The sensitivity of the autoxidation of 2,4,5-trihydroxyphenethylamine (6-hydroxydopamine) to increase by laccase was studied. The autoxidation of 6-hydroxydopamine proceeds by a free radical chain reaction involving O2- and produces the corresponding chromogen 6-hydroxydopaminequinone and hydrogen peroxide. The ability of laccase to increase the autoxidation of 6-hydroxydopamine at pH 5.5 has been used as sensitive assay for this enzyme. The results are tabulated and discussed.
Padiglia
A
A
Istituto di Chimica Biologica, Università di Cagliari.
Medda
R
R
Rescigno
A
A
Floris
G
G
D006863:Hydrogen-Ion Concentration; D042845:Laccase; D010084:Oxidation-Reduction; D016627:Oxidopamine / Q000737:chemistry*; D010088:Oxidoreductases / Q000032:analysis*; D010101:Oxygen Consumption; D010936:Plant Extracts / Q000737:chemistry; D010947:Plants, Toxic; D013053:Spectrophotometry / Q000379:methods; D012251:Toxicodendron
D010936:Plant Extracts; D016627:Oxidopamine; D010088:Oxidoreductases; D042845:Laccase