Pancreastatin molecular forms in normal human plasma.
Circulating molecular forms with pancreastatin (PST)-like immunoreactivity in plasma from normal subjects were examined. An immunoreactive form corresponding to a human PST-like sequence [human chromogranin-A-(250-301)] (hPST-52) and a larger form (mol wt 15-21 kDa) were detected by gel filtration of plasma from normal subjects. On high performance liquid chromatography, predominant immunoreactive forms coeluted with the three larger forms which were purified from the xenograft of human pancreatic islet cell carcinoma cell line QGP-1N cells and with synthetic hPST-52. The fraction containing larger forms purified from xenograft of QGP-1N cells had biological activity equivalent to that of hPST-52 on the inhibition of pancreatic exocrine secretion. These results suggest that the larger molecular forms as well as hPST-52 may be physiologically important circulating forms of PST in human.
Kitayama
N
N
First Department of Biochemistry, School of Medicine, Fukuoka University, Japan.
Tateishi
K
K
Funakoshi
A
A
Miyasaka
K
K
Shimazoe
T
T
Kono
A
A
Iwamoto
N
N
Matsuoka
Y
Y
D000818:Animals; D001681:Biological Assay; D018273:Carcinoma, Islet Cell / Q000378:metabolism*; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010187:Pancreatic Hormones / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D010189:Pancreatic Juice / Q000187:drug effects / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D053379:Chromogranin A; D010187:Pancreatic Hormones; C050836:pancreastatin