Evidence of post-transcriptional regulation in mammalian mitochondrial biogenesis.
We have previously shown that the nuclear-encoded gene's expression of mitochondrial beta-subunit of the F1-ATPase complex in rat liver is regulated at the translational level (Luis, A.M., Izquierdo, J.M., Ostronoff, L.K., Santarén, J., Salinas, M., and Cuezva, J.M. (1993) J. Biol. Chem. 268, 1868-1875). In this paper we report that the different steady-state levels of ATP synthase beta subunit mRNA detected in rat tissues are not paralleled by a proportional content of immunodetectable beta-F1-ATPase protein. The results suggest that tissue-specific transcriptional and post-transcriptional mechanisms contribute to differential mitochondrial biogenesis in mammalian cells. On the other hand, steady-state mRNA levels of the mitochondrial encoded ATP synthase subunits (ATP 6+8) indicate that nuclear and mitochondrial-encoded transcripts for this complex are in close relation, that is, the expression of both nuclear and mitochondrial genes is coordinated in all tissues examined.
Izquierdo
J M
JM
Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain.
Cuezva
J M
JM
D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D014176:Protein Biosynthesis; D006180:Proton-Translocating ATPases / Q000096:biosynthesis*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D014158:Transcription, Genetic
D012333:RNA, Messenger; D006180:Proton-Translocating ATPases