Standardization of pollen allergens of Parthenium hysterophorus and selection of an in-house reference extract.
A standardized in-house reference extract from the pollen of Parthenium hysterophorus, which is responsible for the high incidence of allergic rhinitis in India, was generated and examined by skin test, radio-allergosorbent test inhibition and isoelectric focusing. Parthenium reference allergen discs and positive reference serum were also generated. These reference reagents could not only be used for the quantitation of Parthenium-specific IgE in the sera of rhinitis patients but also for the evaluation of allergenic activity (relative potency and lot-to-lot variation) of different batches of Parthenium pollen.
Sriramarao
P
P
Department of Biochemistry, St Martha's Hospital, Bangalore, India.
Schroder
H
H
Rao
B S
BS
Prakash
O
O
Rao
P V
PV
D000328:Adult; D000485:Allergens / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000276:immunology / Q000592:standards*; D011058:Pollen / Q000276:immunology*; D011852:Radioallergosorbent Test / Q000592:standards*; D012015:Reference Standards; D012220:Rhinitis / Q000175:diagnosis / Q000276:immunology; D012882:Skin Tests
D000485:Allergens; D010936:Plant Extracts; D007073:Immunoglobulin E