title: Development of a shape vector that identifies critical forms assumed by human polymorphonuclear neutrophils during chemotaxis.
abstract: Human polymorphonuclear neutrophils undergo characteristic shape changes that are critical to their ability to move and ingest their targets. We present here the construction of a simple shape vector, calculated from the coordinates of the cell perimeter, that can identify critical forms that a neutrophil assumes during the course of ameboid movement. The vector can be used to find neutrophils of a specific shape from the image analyzer data produced during a typical neutrophil tracking experiment.
authors.affiliation: Division of Pediatric Hematology/Oncology, Childrens Hospital of Los Angeles, University of Southern California School of Medicine 90027.
authors.forename: R S
authors.initials: RS
authors.lastname: Hartman
authors.forename: K
authors.initials: K
authors.lastname: Lau
authors.forename: W
authors.initials: W
authors.lastname: Chou
authors.forename: T D
authors.initials: TD
authors.lastname: Coates
mesh_terms: D000546:Amanitins; D048429:Cell Size; D002634:Chemotaxis, Leukocyte; D005456:Fluorescent Dyes; D006801:Humans; D008433:Mathematics; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D009504:Neutrophils / Q000502:physiology / Q000648:ultrastructure*; D014966:Xanthenes
chemical_list: D000546:Amanitins; D005456:Fluorescent Dyes; D014966:Xanthenes; C028877:7-nitrobenz-2-oxa-1,3-diazole-phallacidin; C034657:Texas red