Intravenous injection of tridihomo-gamma-linolenoyl-glycerol into mice and its effects on delayed-type hypersensitivity.
Highly purified tridihomo-gamma-linolenoyl-glycerol (DGLA-TG) was emulsified with egg yolk lecithin as a 10% (wt/vol) DGLA-TG emulsion. We injected 0.05 or 0.5 mL of the emulsion into mice through the tail vein and investigated its effects on the fatty acid composition of spleen cells and on delayed-type hypersensitivity (DTH) response. At 1 h after the injection, dihomo-gamma-linolenic acid (DGLA) concentrations were increased significantly in the total phospholipid fraction of spleen cells from 1.21 +/- 0.13 mol% to 2.09 +/- 0.74 mol% (P < 0.02) and 7.95 +/- 1.25 mol% (P < 0.001) in the 0.05-mL and 0.5-mL groups, respectively. Mice, which had already been immunized with sheep red blood cells (SRBC), were challenged by the injection of SRBC into the right-hind footpad. Intravenous injection into mice with 0.5 mL of the emulsion immediately before the challenge almost completely suppressed DTH response measured by the swelling of the right-hind footpads 24 h thereafter. This inhibitory effect on the DTH response was significant with as little as 0.05 mL of the emulsion, whereas a soybean oil emulsion was not effective at all. In conclusion, intravenous injection of a DGLA emulsion increased DGLA concentrations in immune cells within 1 h and suppressed the DTH reaction.
Taki
H
H
First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Japan.
Nakamura
N
N
Hamazaki
T
T
Kobayashi
M
M
D015126:8,11,14-Eicosatrienoic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006968:Hypersensitivity, Delayed; D007275:Injections, Intravenous; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014280:Triglycerides / Q000008:administration & dosage / Q000494:pharmacology*
D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides; C081568:tridihomo-gamma-linolenoylglycerol; D015126:8,11,14-Eicosatrienoic Acid