title: Solution structure of bovine heart fatty acid-binding protein (H-FABPc).
abstract: Fatty acid-binding protein (FABP) from bovine heart, a 15 kDa cytoplasmic protein has been investigated by multi-dimensional homonuclear and heteronuclear NMR-spectroscopy. Perdeuterated palmitic acid has been used as fatty acid ligand. The tertiary structure has been determined from distance geometry calculations with the variable target functions algorithm (DIANA) utilizing 1027 interproton distance constraints, which were obtained from 1H-homonuclear NOESY spectra. Overlapping NOE crosspeaks were assigned by heteronuclear multidimensional NMR-experiments with a 15N-labelled sample. The tertiary structure resembles a beta-barrel (beta-clam) consisting of ten anti-parallel beta-strands and a short helix-turn-helix motif. The beta-strands are arranged in two nearly orthogonal beta-sheets composed of 5 strands each. The solution structure is compared with the x-ray crystal structure of bovine heart and rat intestinal FABPs.
authors.affiliation: Institut für Biophysikalische Chemie, J.W. Goethe-Universität Frankfurt, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Lassen
authors.forename: C
authors.initials: C
authors.lastname: Lücke
authors.forename: A
authors.initials: A
authors.lastname: Kromminga
authors.forename: A
authors.initials: A
authors.lastname: Lezius
authors.forename: F
authors.initials: F
authors.lastname: Spener
authors.forename: H
authors.initials: H
authors.lastname: Rüterjans
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000737:chemistry*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D017434:Protein Structure, Tertiary; D012996:Solutions
chemical_list: D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012996:Solutions