DNA topoisomerase II and casein kinase II associate in a molecular complex that is catalytically active.
Immunoprecipitation of DNA topoisomerase II from yeast results in a preparation that contains casein kinase II; this suggests that the two proteins may associate in the intact cell. Purified recombinant topoisomerase II and casein kinase II associate to form a complex in vitro which is stable after topoisomerase II becomes phosphorylated by the kinase. Studies with isolated recombinant casein kinase II subunits disclosed that although the alpha (catalytic) subunit alone can efficiently phosphorylate topoisomerase II, the formation of a stable topoisomerase II-casein kinase II association requires the presence of the beta subunit of the kinase. Both proteins engaged in this complex retain their catalytic activities. Naturally occurring polyamines and polyanionic compounds appear to be crucial factors governing the interaction between the two proteins. Although the biological significance of a stable catalytically active topoisomerase II-casein kinase II molecular complex remains to be defined, these observations suggest the possibility of a novel mechanism regulating topoisomerase II and casein kinase II activities.
Bojanowski
K
K
Institut National de la Santé et de la Recherche Médicale Unité 140, Institut Gustave Roussy, Villejuif, France.
Filhol
O
O
Cochet
C
C
Chambaz
E M
EM
Larsen
A K
AK
D000595:Amino Acid Sequence; D000818:Animals; D047390:Casein Kinase II; D004250:DNA Topoisomerases, Type II / Q000737:chemistry / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D006497:Heparan Sulfate / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009036:Moths; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013539:Simian virus 40; D013096:Spermine / Q000494:pharmacology; D013379:Substrate Specificity; D013498:Suramin / Q000494:pharmacology; D014162:Transfection
D047390:Casein Kinase II; D004250:DNA Topoisomerases, Type II; D004279:DNA, Viral; D006493:Heparin; D006497:Heparan Sulfate; D046911:Macromolecular Substances; D017346:Protein Serine-Threonine Kinases; D011994:Recombinant Proteins; D013096:Spermine; D013498:Suramin