Plasminogen-binding protein associated with the plasma membrane of cultured embryonic rat neocortical neurons.
To investigate the receptor-like molecule(s) for plasminogen (PGn) on the neuronal surface, the properties of binding of PGn to the plasma membrane of cultured embryonic rat neocortical neurons were investigated. [125I]PGn was found to specifically bind to the plasma membrane depending on the incubation temperature and time. The binding was also affected strongly by ionic strength and slightly by Ca2+. Furthermore, ligand blotting analysis revealed that [125I]PGn binds to a major protein with an apparent molecular weight of 45 kDa among plasma membrane proteins. These results suggest that the 45-kDa protein is a PGn receptor-like molecule on the neuronal surface.
Nakajima
K
K
Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan.
Nagata
K
K
Hamanoue
M
M
Takemoto
N
N
Shimada
A
A
Kohsaka
S
S
D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D007700:Kinetics; D008970:Molecular Weight; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010958:Plasminogen / Q000378:metabolism*; D011205:Povidone; D051381:Rats; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator; D012822:Silicon Dioxide
C524900:Plaur protein, rat; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; C016039:Percoll; D012822:Silicon Dioxide; D010958:Plasminogen; D011205:Povidone