A novel in vivo model for the study of cartilage degradation.
Methods of quantifying cartilage destruction are described using a sponge/cartilage implant model in the rat. A cylinder of bovine nasal cartilage was positioned in the center of a sponge which had been pretreated with an irritant. The sponge/cartilages were then implanted subcutaneously into the backs of rats for periods of up to 16 days. The implanted sponges were rapidly surrounded by granulation tissue, maximal on day 2, and infiltrated by inflammatory cells which reached peak levels on day 9. Analysis of the cartilage shows an initial increase in wet weight and rapid loss of glycosaminoglycans. These changes were later followed by loss of cartilage wet weight and significant loss of hydroxyproline content. In a separate study, the effects of Mycobacterium tuberculosis (Mtb), kaolin, and zymosan were compared (1 mg/sponge) and the results showed that only Mtb induced pronounced inflammation and degradation of cartilage. The cartilage degradation directly correlated with the granulation tissue weight, but not with cellular infiltration. We believe that this simple, reproducible in vivo model could be used to elucidate the mechanisms involved in the destructive process and evaluate the efficacy of inhibitors of cartilage degradation.
Bishop
J
J
Biology Department, Roche Products Ltd., Welwyn Garden City, Hertfordshire, England, United Kingdom.
Greenham
A K
AK
Lewis
E J
EJ
D000818:Animals; D002357:Cartilage Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D002358:Cartilage, Articular / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D004195:Disease Models, Animal; D006909:Hydroxyproline / Q000378:metabolism; D066298:In Vitro Techniques; D007509:Irritants / Q000633:toxicity; D009300:Nasal Septum / Q000473:pathology; D011509:Proteoglycans / Q000378:metabolism
D007509:Irritants; D011509:Proteoglycans; D006909:Hydroxyproline