Coexistence of somatic and germ-line mutations of APC gene in desmoid tumors from patients with familial adenomatous polyposis.
Desmoid tumors, which are locally invasive with recurrence but without metastasis, are frequently observed in patients with familial adenomatous polyposis after abdominal surgery or during pregnancy. This study analyzed mutation of the adenomatous polyposis coli gene in 8 desmoid tumors from 7 familial adenomatous polyposis patients using polymerase chain reaction-single-strand conformation polymorphism and the direct sequencing method. Seven somatic mutations, 1 somatic allele loss, and 6 germ-line mutations were detected. The majority of adenomatous polyposis coli gene mutations were deletions of 1 to 19 base pairs in exon 15, and all mutations led to the formation of stop codons. A somatic mutation with repetition of 82 base pairs from codon 1399 to 1426 was also observed in a desmoid, which was most likely caused by an error during replication or repair replication. No mutation was detected in exons 1 to 2 of H-ras, K-ras, and N-ras genes and in exons 5 to 8 of p53 gene, in these tumors. The simultaneous existence of somatic and germ-line alterations of adenomatous polyposis coli gene observed in all 8 tumors strongly suggests that inactivation of both alleles of adenomatous polyposis coli gene is involved in the development of desmoid tumors.
Miyaki
M
M
Department of Biochemistry, Tokyo Metropolitan Institute of Medical Science, Japan.
Konishi
M
M
Kikuchi-Yanoshita
R
R
Enomoto
M
M
Tanaka
K
K
Takahashi
H
H
Muraoka
M
M
Mori
T
T
Konishi
F
F
Iwama
T
T
D011125:Adenomatous Polyposis Coli / Q000150:complications / Q000235:genetics*; D000328:Adult; D001483:Base Sequence; D002869:Chromosome Aberrations; D003062:Codon / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics; D005091:Exons; D005260:Female; D018222:Desmoid Tumors / Q000150:complications / Q000235:genetics*; D017491:Genes, APC; D011905:Genes, ras; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D017384:Sequence Deletion
D003062:Codon