title: Production of cytokines by mouse peritoneal cells treated with Tołpa Torf Preparation (TTP): dependence on age and strain of mice.
abstract: Production of two cytokines: Interferon beta (IFN-beta) and tumor necrosis factor alpha (TNF-alpha) by freshly isolated resident mouse peritoneal cells (RPC) treated with immunostimulating drug Tołpa* Torf Preparation (TTP) was investigated. Nontoxic concentrations of TTP (10 and 100 micrograms/ml) potentiated the cytokine production by RPC of BALB/c mice. The observed effects were dose-dependent. The optimal cytokine-inducing concentration of TTP was 100 micrograms/ml. The effect of TTP depended on the age of mice. The high potentiation (16-fold) of IFN-beta and TNF-alpha production by TTP was observed when 5 and 8 week-old mice were used. However, in RPC isolated from one year old mice, only two (fold) potentiation of IFN and TNF was observed. The activities of the same commercial preparations of TTP were compared in the RPC cultures isolated from mice of the three different strains: BALB/c, C3H/HeJ and NZB. The significant stimulation (16-32-fold) of TNF and IFN was observed in the cells isolated from BALB/c mice. In contrast, only slight stimulation of IFN and TNF (1-3-fold) was observed in cells of NZB mice. Peritoneal cells of C3H mice did not respond to stimulation with TTP.
authors.affiliation: Laboratory of Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
authors.forename: Z
authors.initials: Z
authors.lastname: Błach-Olszewska
authors.forename: E
authors.initials: E
authors.lastname: Zaczyńska
authors.forename: E
authors.initials: E
authors.lastname: Broniarek
authors.forename: A D
authors.initials: AD
authors.lastname: Inglot
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000367:Age Factors; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D002478:Cells, Cultured; D004338:Drug Combinations; D005260:Female; D006812:Humic Substances / Q000494:pharmacology*; D007369:Interferon Inducers / Q000494:pharmacology*; D016899:Interferon-beta / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008814:Mice, Inbred NZB; D010529:Peritoneal Cavity / Q000166:cytology; D012987:Soil / Q000032:analysis*; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D014574:Uronic Acids / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000596:Amino Acids; D002241:Carbohydrates; D004338:Drug Combinations; D006812:Humic Substances; D007369:Interferon Inducers; D012987:Soil; C084029:Tolpa; D014409:Tumor Necrosis Factor-alpha; D014574:Uronic Acids; D016899:Interferon-beta