title: Localization of indium-111-immunoglobulin G, technetium-99m-immunoglobulin G and indium-111-labeled white blood cells at sites of acute bacterial infection in rabbits.
abstract: Biodistribution and infection imaging properties of 111In-DTPA-IgG, 99mTc-hydrazino nicotinamide-IgG and 111In-WBC were compared in rabbits with E. coli infection. Groups of six rabbits were injected with 10 mCi of 99mTc-IgG plus 0.5 mCi of 111In-IgG or 1 mCi of 99mTc-IgG plus 0.05 mCi of 111In-WBC. At 4-5 and 18-20 hr, dual photon scintigrams were acquired. At both times, the distributions of 99mTc and 111In-IgG were nearly identical. The sites of infection were well visualized with all three radiopharmaceuticals. In the early images, the target-to-background ratios (T/B) for 111In and 99mTc-IgG determined by ROI analysis were 1.95 +/- 0.26 and 2.57 +/- 0.38 (p = NS). In the delayed images, the T/B ratios increased (p < 0.01) to 3.56 +/- 0.49 and 4.90 +/- 0.98. At both times, the T/B ratios for 111In-WBC were higher (p < 0.01); 4.17 +/- 0.78 at 4-5 hr and 8.52 +/- 1.52 at 18-20 hr. These results indicate that all three agents yield excellent images of infection sites. Although 111In-WBC had higher T/B ratios, the ease of preparation of the radiolabeled proteins makes them attractive alternatives for infection imaging.
authors.affiliation: Department of Radiology, Massachusetts General Hospital, Boston 02114.
authors.forename: S A
authors.initials: SA
authors.lastname: Barrow
authors.forename: W
authors.initials: W
authors.lastname: Graham
authors.forename: S
authors.initials: S
authors.lastname: Jyawook
authors.forename: S C
authors.initials: SC
authors.lastname: Dragotakes
authors.forename: H F
authors.initials: HF
authors.lastname: Solomon
authors.forename: J W
authors.initials: JW
authors.lastname: Babich
authors.forename: R H
authors.initials: RH
authors.lastname: Rubin
authors.forename: A J
authors.initials: AJ
authors.lastname: Fischman
mesh_terms: D000208:Acute Disease; D000818:Animals; D004927:Escherichia coli Infections / Q000000981:diagnostic imaging*; D007074:Immunoglobulin G / Q000378:metabolism; D007205:Indium Radioisotopes / Q000493:pharmacokinetics; D007962:Leukocytes; D008297:Male; D008961:Models, Structural; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D011817:Rabbits; D011877:Radionuclide Imaging; D014018:Tissue Distribution
chemical_list: D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D015609:Organotechnetium Compounds; C079358:technetium TC 99m polyclonal IgG