title: [Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)].
abstract: Two antigens of polysaccharidic nature were evaluated for their specificity in serodiagnosis of porcine pleuropneumonia (serotype 5). Recently, long chains lipopolysaccharides (LC-LPS) was shown to increase the specificity of the ELISA test for A. pleuropneumoniae serotype 5 compared to a saline extract of boiled-formalinized whole cells. Nevertheless, some cross reactions were noticed with LC-LPS. We have thus cleaved the LPS molecule at the ketosidic bond between the lipid A and the core. The lipid A was separated from the polysaccharide (PS) by centrifugation. With an indirect sandwich ELISA, it was possible to demonstrate that the PS yielded positive responses with homologous sera (serotype 5) and negative responses with heterologous sera (serotype 3 and undeterminated serotype). Overall, the use of the PS did not notably increase the specificity of the ELISA test. Since production of PS from LC-LPS and its separation from the lipid A is not simple, we do not recommend its use in serodiagnosis.
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Bada Alambedji
authors.forename: J D
authors.initials: JD
authors.lastname: Dubreuil
mesh_terms: D000189:Actinobacillus Infections / Q000175:diagnosis / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D011001:Pleuropneumonia / Q000175:diagnosis / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial
vernacular_title: Comparaison de deux antigènes de nature polysaccharidique pour le diagnostic sérologique par ELISA de la pleuropneumonie porcine (sérotype 5).