Signal transduction in Trypanosoma cruzi: opposite effects of adenylcyclase and phospholipase C systems in growth control.
Fetal calf serum (FCS), which is mitogenic for the pathogenic protozoa T. cruzi, inhibits cAMP production in basal and forskolin-stimulated epimastigotes. It also activates phosphoinositides hydrolysis yielding diacylglycerol and inositol phosphates (Ins-P). Ins-P production is enhanced by AlF4-, GTP or beta-gamma-methylene-GTP, thus implying G proteins mediation in the phenomenon. An enzyme with phospholipase C activity which may be involved in the phospholipid metabolism was partially characterized.
Oliveira
M M
MM
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Rocha
E D
ED
Rondinelli
E
E
Arnholdt
A V
AV
Scharfstein
J
J
D000262:Adenylyl Cyclases / Q000502:physiology*; D000818:Animals; D001790:Blood Physiological Phenomena; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D007295:Inositol Phosphates / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000502:physiology*; D015800:Protozoan Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000502:physiology*
D007295:Inositol Phosphates; D008563:Membrane Lipids; D010716:Phosphatidylinositols; D015800:Protozoan Proteins; D005576:Colforsin; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases; D043265:Phosphatidylinositol Diacylglycerol-Lyase