Demonstration of melatonin-binding sites in cyclohexylamine-formaldehyde-fixed brain tissues.
This study shows for the first time that perfusion of rat or hamster brain with a cyclohexylamine-paraformaldehyde mixture makes possible the observation by autoradiography of melatonin binding sites in structurally well-preserved fixed tissues. This result is a first step in the identification of melatonin-receptor-containing cell types by cytoautoradiography.
Masson-Pévet
M
M
Neurobiologie des Fonctions Rythmiques et Saisonniéres, URA-CNRS 1332, Université Louis Pasteur, Strasbourg, France.
George
D
D
Gauer
F
F
Pévet
P
P
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D006224:Cricetinae; D003514:Cyclohexylamines; D005404:Fixatives; D005557:Formaldehyde; D008297:Male; D008550:Melatonin / Q000378:metabolism*; D008647:Mesocricetus; D010903:Pituitary Gland, Anterior / Q000033:anatomy & histology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000033:anatomy & histology / Q000378:metabolism; D016707:Tissue Fixation / Q000379:methods
D003514:Cyclohexylamines; D005404:Fixatives; D005557:Formaldehyde; D008550:Melatonin