Systematic HRAS amplification in ovary-independent mammary tumors: correlation with progressively anaplastic phenotypes.
Progression of well differentiated rat mammary adenocarcinomas to very anaplastic phenotypes was found to correlate with a systematic and significant amplification of a mutant HRAS allele. Tumors with high amplification levels of this oncogene were analyzed by chromosomal in situ hybridization; in four of the cases the amplified sequences did not reside at the native chromosome 1 locus but were localized in a novel marker chromosome. The model described has potential as a reproducible system for the study of the chromosomal and cellular mechanisms operative "in vivo" for oncogene amplification.
Aldaz
C M
CM
University of Texas M. D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957.
Gollahon
L S
LS
Chen
A
A
D000230:Adenocarcinoma / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D000483:Alleles; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002277:Carcinoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D002871:Chromosome Banding; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D017403:In Situ Hybridization; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008770:Methylnitrosourea; D010641:Phenotype; D051381:Rats; D011915:Rats, Inbred BUF
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D008770:Methylnitrosourea