title: Nucleotide sequence and E. coli expression of the coat protein gene of the yellowing strain of soybean dwarf luteovirus.
abstract: The nucleotide sequence of the coat protein gene of the yellowing (Y) strain of soybean dwarf virus (SbDV) was determined from cloned cDNA. The gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated M(r) of 22,200. The identity of the open reading frame (ORF) encoding the coat protein was confirmed by expression of an Escherichia coli beta-galactosidase fusion protein and detection by a dot blot immunoassay. Sequence comparisons of the deduced coat protein amino acids to several luteoviruses demonstrated that the SbDV-Y coat protein ORF shares greatest similarity with bean leaf roll virus (BLRV) (65%).
authors.affiliation: USDA-ARS, Fort Detrick, Frederick, Maryland.
authors.forename: O P
authors.initials: OP
authors.lastname: Smith
authors.forename: V D
authors.initials: VD
authors.lastname: Damsteegt
authors.forename: K F
authors.initials: KF
authors.lastname: Harris
authors.forename: R
authors.initials: R
authors.lastname: Vonder Haar
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004279:DNA, Viral; D004926:Escherichia coli; D017793:Luteovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D011994:Recombinant Proteins; C084189:coat protein, soybean dwarf virus (yellowing)