title: DNA curvature influences the internal motions of supercoiled DNA.
abstract: We present evidence that short curved DNA segments can act as mediators for the ordering of large domains in superhelical DNA. Using a non-invasive solution method (dynamic light scattering), we investigated the effect of permanently curved inserts on the solution structure and on the internal motions of superhelical plasmid DNA. We find that the dynamics of superhelical DNA are strongly influenced by sequence- or protein-induced bending: in superhelical plasmids containing curved inserts the amplitude of the internal motion is lower than that of non-curved controls. Furthermore, the relative arrangement of curved sequences in the plasmids can influence the overall shape of the superhelical DNA. On linearized forms of the plasmids, these effects are not observed.
authors.affiliation: EMBL, Grenoble Outstation, France.
authors.forename: W
authors.initials: W
authors.lastname: Kremer
authors.forename: K
authors.initials: K
authors.lastname: Klenin
authors.forename: S
authors.initials: S
authors.lastname: Diekmann
authors.forename: J
authors.initials: J
authors.lastname: Langowski
mesh_terms: D001483:Base Sequence; D003198:Computer Simulation; D004269:DNA, Bacterial / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry*; D004058:Diffusion; D008969:Molecular Sequence Data; D009010:Monte Carlo Method; D009038:Motion; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D004278:DNA, Superhelical