Presence of telomeric sequences on deleted chromosomes and their absence on double minutes in cell line HL-60.
The issue of telomeric sequences on deleted chromosomes and double minutes (dmin) was investigated by examining the cell line HL-60 with fluorescence in situ hybridization using a human plasmid DNA sequence with 800 bp TTAGGG repeats. This cell line showed telomeric sequences on the deleted short arms of chromosomes 9 and 10, with the results suggesting that so-called terminal deletion may be, in fact, an interstitial deletion, or that telomeric sequences may be synthesized by telomerase after deletion. On the other hand, numerous dmin showed no evidence of hybridization with the telomeric probe. This suggests that the characteristics unequal distribution of dmin during mitosis may result from the lack of not only centromeres but also telomeres.
Furuya
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Cancer Center, Southwest Biomedical Research Institute, Scottsdale, Arizona 85251.
Morgan
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Berger
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CS
Sandberg
A A
AA
D002872:Chromosome Deletion; D002879:Chromosomes, Human, Pair 10 / Q000648:ultrastructure*; D002899:Chromosomes, Human, Pair 9 / Q000648:ultrastructure*; D015342:DNA Probes; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D016615:Telomere; D014407:Tumor Cells, Cultured
D015342:DNA Probes