Identification of a lytic-phase origin of DNA replication in human herpesvirus 6B strain Z29.
DNA sequences which have structural features suggestive of their functioning as an origin of lytic-phase DNA replication were previously identified in both human herpesvirus 6B strain Z29 [HHV-6B (Z29)] and in HHV-6A (U1102). Plasmid constructs containing the putative HHV-6B (Z29) oriLyt element were replicated after transfection into permissive T cells, when trans-acting factors were provided by HHV-6B (R-1) infection. By using this assay, the HHV-6B (Z29) oriLyt was mapped to a minimal region of approximately 400 bp which lies upstream of the gene that is homologous to herpes simplex virus UL29, a region that carries an origin in other betaherpesviruses and in some alphaherpesviruses.
Dewhurst
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Department of Microbiology and Immunology, University of Rochester, New York 14642.
Dollard
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Pellett
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Dambaugh
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D004252:DNA Mutational Analysis; D004261:DNA Replication / Q000235:genetics*; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000254:growth & development; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA; D014779:Virus Replication
D004274:DNA, Recombinant; D004279:DNA, Viral