Distribution of vinculin in the duodenum of chick embryos: immunohistochemical studies at the light and electron microscopic level.
Studies on the localization of vinculin and actin in the embryonic chick duodenum between the 9th and 13th days of incubation were performed by light and electron microscopy. In the light microscopic studies, vinculin and actin were localized by immuno-fluorescent double staining with anti-vinculin and rhodamine phalloidin for actin. In the electron microscopic studies, the immunocytochemical distribution of vinculin was examined on Lowicryl K4M thin sections with anti-vinculin. Vinculin was prominent near or adjacent to the apical inner membrane of epithelial cells of the previllous ridges in all stages, often located near cell junctions including tight and adherent junctions and the top of microvilli, and weakly in the cytoplasm. Our findings suggested the possible interaction between actin and vinculin in differentiation on the apical side of epithelial cells including the formation of microvilli or terminal webs.
Noda
S
S
Department of Morphology, School of Medicine, Tokai University, Iseha, Japan.
Mitsui
T
T
D000199:Actins / Q000032:analysis; D000818:Animals; D002642:Chick Embryo / Q000737:chemistry*; D004386:Duodenum / Q000737:chemistry* / Q000196:embryology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D016596:Vinculin / Q000032:analysis*
D000199:Actins; D016596:Vinculin