[Fermentation of engineered strain producing cholera toxin B subunit].
Studies indicate that the cholera B subunit could be synthesized efficiently in corn steep liquor by engineered E. coli strain MM2. We have also determined the optical density, pH and the yield of B subunit kinetically during fermentation. Corn steep liquor medium has advantages in low cost, simplicity in technological process and high yield of B subunit which can reaches 40 micrograms/ml in 50L fermentative tank.
Yu
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X
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing.
Ma
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Q
D002772:Cholera Toxin / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation; D005818:Genetic Engineering; D010446:Peptide Fragments / Q000096:biosynthesis*; D003313:Zea mays
D010446:Peptide Fragments; C050310:cholera toxin, B subunit (50-64); D002772:Cholera Toxin