[Comparative susceptibility of different cell lines for culture of Toxoplasma gondii in vitro].
In order to establish a useful cell culture system for T. gondii, we compared the degree of proliferation of T. gondii tachyzoites among 8 different cell lines; 2 kinds of normal animal cells (MDCK-canine kidney cells; Vero-monkey kidney cells) and 6 kinds of human tumor cells (A 549, PC 14-lung cancer cells; SNU 1, SNU 16, MKN 45-stomach cancer cells; HL-60-promyelocytic leukemia cells), through morphological observation and 3H-uracil uptake assay. The degree of susceptibility to infection with T. gondii tachyzoites was highest in A 549 and PC 14 cells, medium in Vero, HL-60, MDCK and SNU 1, and lowest in SNU 16 and MKN 45 cells. The kinetics of T. gondii multiplication during the post-infection 60 hours were highly dependent upon the dose of tachyzoites administered and the duration of cultivation. These results show that A 549 and PC 14 are the most suitable cell lines among the 8 tested for the growth and multiplication of T. gondii in vitro.
Park
B K
BK
Department of Pediatrics, Gyeongsang National University, Chinju, Korea.
Moon
H R
HR
Yu
J R
JR
Kook
J
J
Chai
J Y
JY
Lee
S H
SH
D000818:Animals; D002460:Cell Line / Q000469:parasitology; D004285:Dogs; D000882:Haplorhini; D006801:Humans; D013997:Time Factors; D014122:Toxoplasma / Q000254:growth & development*; D014407:Tumor Cells, Cultured / Q000469:parasitology