title: Neutrophil chemotactic activity (NCA) in nasal secretions from atopic and nonatopic subjects. Effect of antigen challenge.
abstract: In order to elucidate the mechanism responsible for infiltration of nasal mucosa by granulocytes, we tested neutrophil chemotactic activity (NCA) in nasal lavages, by the modified Boyden chamber method, in 16 patients with perennial allergic rhinitis (AR), six ASA-sensitive patients with chronic rhinosinusitis (CRS), and seven normal, nonatopic control subjects (NC). Nasal secretions from all three groups showed significant NCA (mean 157.1 +/- 54.0, 62.2 +/- 20.7, and 39.4 +/- 11.4% of FMLP chemotactic activity for AR, CRS, and NC subjects, respectively). Nasal secretions from patients with AR expressed significantly higher NCA (P < 0.02) than did secretions from NA patients. NCA was unchanged by heating at 56 degrees C for 60 min and was not susceptible to degradation by trypsin. Nasal challenge with Dermatophagoides pteronyssinus antigen induced clinical symptoms and resulted in significant increases in total protein and albumin concentrations in nasal lavages in AR patients, but failed to change the mean NCA activity for up to 40 min after the challenge. These results indicate that nasal secretions from both atopic and nonatopic patients express NCA, but its relation to allergic inflammation remains to be established.
authors.affiliation: Department of Pulmonology and Allergology, Faculty of Medicine, Medical Academy, Lódź, Poland.
authors.forename: M L
authors.initials: ML
authors.lastname: Kowalski
authors.forename: J
authors.initials: J
authors.lastname: Grzegorczyk
authors.forename: M
authors.initials: M
authors.lastname: Sliwinska-Kowalska
authors.forename: B
authors.initials: B
authors.lastname: Wojciechowska
authors.forename: M
authors.initials: M
authors.lastname: Rozniecka
authors.forename: J
authors.initials: J
authors.lastname: Rozniecki
mesh_terms: D000328:Adult; D000485:Allergens / Q000009:adverse effects*; D002630:Chemotactic Factors / Q000276:immunology*; D002633:Chemotaxis; D002908:Chronic Disease; D006801:Humans; D017817:Nasal Lavage Fluid; D009297:Nasal Mucosa / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology*; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D012852:Sinusitis / Q000276:immunology*
chemical_list: D000485:Allergens; D002630:Chemotactic Factors