PAF activation of a voltage-gated R-type Ca2+ channel in human and canine aortic endothelial cells.
By the use of fura-2 and digital imaging techniques, [K]o depolarization or PAF (10(-9) M) were shown to induce a sustained increase of [Ca]i in human or canine single aortic vascular endothelial cells (VEC) that was insensitive to nifedipine but sensitive to (-)-PN200-110 or to lowering of [Ca]o. The PAF-induced effect on [Ca]i was blocked by the PAF receptor antagonist, WEB2170. Our results suggest that [K]o depolarization and PAF increase [Ca]i via the activation of R-type Ca2+ channels.
Bkaily
G
G
Department of Physiology and Biophysics, Faculty of Medicine, University of Sherbrooke, Québec, Canada.
d'Orléans-Juste
P
P
Naik
R
R
Pérodin
J
J
Stankova
J
J
Abdulnour
E
E
Rola-Pleszczynski
M
M
D001381:Azepines / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000378:metabolism* / Q000502:physiology; D004594:Electrophysiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D016257:Fura-2; D006801:Humans; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000502:physiology*; D017275:Isradipine / Q000494:pharmacology; D008297:Male; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
D001381:Azepines; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D010972:Platelet Activating Factor; D014230:Triazoles; C063964:bepafant; D011188:Potassium; D016257:Fura-2; D017275:Isradipine