title: Translational control by influenza virus. Selective translation is mediated by sequences within the viral mRNA 5'-untranslated region.
abstract: In cells infected by influenza virus type A, host cell protein synthesis declines rapidly and dramatically, while influenza viral protein synthesis occurs efficiently throughout infection. Previously, we had shown that the selective translation of influenza viral mRNAs in infected cells occurred in a cap-dependent manner and was due at least in part to structures inherent in the mRNAs. Using chimeras containing the noncoding and coding regions of cellular and viral mRNAs, we can now report that the selective translation is mediated by sequences within the 5'-untranslated regions (UTR) of the viral mRNAs. Polysome analysis confirmed that a 45-nucleotide sequence contained in the 5'-UTR of the influenza viral nucleocapsid protein was necessary and sufficient to allow the host cell translational machinery to discriminate between viral and cellular mRNAs. In reciprocal experiments in which the 5'-UTR of the cellular mRNA-secreted embryonic alkaline phosphatase replaced the nucleocapsid protein 5'-UTR, viral protein synthesis was inhibited in virus-infected cells, resembling host protein synthesis. Finally, we demonstrated that the 5'-UTR of another influenza viral mRNA, that encoding the nonstructural protein, also conferred resistance to the shutoff of protein synthesis in influenza virus-infected cells.
authors.affiliation: Department of Microbiology, School of Medicine, University of Washington, Seattle 98195.
authors.forename: M S
authors.initials: MS
authors.lastname: Garfinkel
authors.forename: M G
authors.initials: MG
authors.lastname: Katze
mesh_terms: D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis*; D002460:Cell Line; D002678:Chimera; D002522:Chlorocebus aethiops; D017931:DNA Primers; D004285:Dogs; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism*; D007668:Kidney; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral