title: Induction of pyruvate decarboxylase in glycolysis mutants of Saccharomyces cerevisiae correlates with the concentrations of three-carbon glycolytic metabolites.
abstract: Pyruvate decarboxylase, PDCase, activity in wild-type yeast cells growing on ethanol is quite low but increases up to tenfold upon addition of glucose, less with galactose and only slightly with glycerol. PDCase levels in glycolysis mutant strains growing on ethanol or acetate were higher than in the wild-type strain. These levels correlated with the sum of the concentrations of three-carbon glycolytic metabolites. The highest accumulation was observed in a fructose bisphosphate aldolase deletion mutant concomitant with the highest PDCase activity ever observed under gluconeogenic conditions. Glucose addition induced an increase in PDCase activity in all mutants. However, the enzyme activities never reached wild-type level. On the other hand, the PDCase levels in the different mutants again correlated with the sum of the concentrations of the three-carbon glycolytic metabolites. This was interpreted to mean that full induction of PDCase activity requires the accumulation of hexose- and triosephosphates.
authors.affiliation: Institut für Mikrobiologie, Technische Hochschule, Darmstadt, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Boles
authors.forename: F K
authors.initials: FK
authors.lastname: Zimmermann
mesh_terms: D002244:Carbon / Q000378:metabolism; D004790:Enzyme Induction / Q000502:physiology; D006019:Glycolysis / Q000502:physiology*; D009154:Mutation; D011767:Pyruvate Decarboxylase / Q000096:biosynthesis / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D002244:Carbon; D011767:Pyruvate Decarboxylase