Expression of interleukin-1 alpha and interleukin-1 receptor type I genes in murine brown adipose tissue.
At thermoneutral conditions, high steady-state levels of transcripts for both IL-1 alpha and its receptor IL-1RtI were found in specialized thermogenic organ, brown adipose tissue (BAT) of adult mice, as compared with the levels in lymph nodes, brain and spleen. A pronounced decrease of IL-1 alpha mRNA level in BAT was observed after lipopolysaccharide (LPS) administration and after exposure to cold. Likewise, LPS decreased the IL-1RtI mRNA level and depressed also the expression of cold-inducible genes for the BAT-specific heat-producing uncoupling protein and for lipoprotein lipase. It is concluded that, besides the centrally-mediated effects, there exists a direct peripheral interaction of IL-1 cytokines with BAT cells.
Burýsek
L
L
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
Tvrdík
P
P
Houstĕk
J
J
D000199:Actins / Q000096:biosynthesis; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D015152:Blotting, Northern; D001833:Body Temperature Regulation / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis*; D013154:Spleen / Q000378:metabolism
D000199:Actins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1