Isolation of a cDNA clone, encoding the ribosomal protein S20, downregulated during the onset of apoptosis in a human leukaemic cell line.
In this report we describe the isolation, by differential screening, and characterization of a cDNA clone downregulated fivefold in association with the induction of apoptosis in the human leukaemic cell line CEM C7. DNA sequence analysis identified this clone as representing the gene encoding the S20 ribosomal protein. Interestingly, the expression of the S20 mRNA was downregulated early during the induction of apoptosis in this model system, prior to the onset of DNA fragmentation and other morphological changes associated with cell death, suggesting some degree of involvement of this particular gene in the biochemical events that occur during the onset of cell death.
Goldstone
S D
SD
Department of Pathology, University of Sydney, NSW, Australia.
Lavin
M F
MF
D017209:Apoptosis / Q000528:radiation effects; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000737:chemistry / Q000302:isolation & purification*; D005720:Gamma Rays; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006801:Humans; D007938:Leukemia; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000302:isolation & purification; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012269:Ribosomal Proteins; C029611:ribosomal protein S20