title: Molecular design of artificial hydrolytic nucleases and ribonucleases.
abstract: Artificial nucleases and ribonucleases, which selectively hydrolyze the specific phosphodiester linkages in DNAs and RNAs, are prepared by the attachment of organic and inorganic catalysts to DNA oligomers as sequence-recognizing moieties. A hybrid of ethylenediamine and a 19-mer DNA selectively hydrolyzes tRNA(Phe) at the 3'-side of C63. Site-selective hydrolysis of DNAs has been accomplished by the lanthanide complexes bound to DNA oligomers. No concurrent oxidative cleavage of the ribose or the deoxyribose takes place.
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Komiyama
authors.forename: T
authors.initials: T
authors.lastname: Inokawa
authors.forename: T
authors.initials: T
authors.lastname: Shiiba
authors.forename: N
authors.initials: N
authors.lastname: Takeda
authors.forename: K
authors.initials: K
authors.lastname: Yoshinari
authors.forename: M
authors.initials: M
authors.lastname: Yashiro
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D003851:Deoxyribonucleases / Q000138:chemical synthesis* / Q000737:chemistry; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012360:RNA, Transfer, Phe / Q000737:chemistry*; D012260:Ribonucleases / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D012360:RNA, Transfer, Phe; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases