Characterization of the products of dinucleoside monophosphates d(GpN) irradiated in aqueous solutions.
The dinucleoside monophosphates d(GpT), d(GpC), and d(GpA) were X-irradiated in oxygenated aqueous solution. The principal products were identified in the intact modified dinucleoside monophosphates using NMR spectroscopy and FAB mass spectrometry. The 8-hydroxyguanine modification is a major product in each of the d(GpN). The d(GpN), where N is a pyrimidine nucleoside, also yield products in which the pyrimidine base is degraded to a formamido remnant. The most interesting product is one bearing two base damages, 8-hydroxylation of the guanine base and degradation of the pyrimidine base to a formamido remnant. This double lesion was observed in both d(GpN) where N is a pyrimidine base.
Budzinski
E E
EE
Department of Experimental Therapeutics, Roswell Park Cancer Institute, Buffalo, New York 14263.
Maccubbin
A E
AE
Freund
H G
HG
Wallace
J C
JC
Box
H C
HC
D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D015226:Dinucleoside Phosphates / Q000528:radiation effects*; D009682:Magnetic Resonance Spectroscopy
D015226:Dinucleoside Phosphates