Variations in temperature and oxygen content do not alter levels of thiobarbiturate reactive material in human breast tumour cells (ZR-75-1) incubated with gamma-linolenic acid.
The mechanism by which tumour cells may be killed in vitro by exogenous polyunsaturated fatty acids may involve lipid peroxidation. Gamma-linolenic acid caused a dose and time-dependent reduction in ZR-75-1 cell growth. However, altering either the incubator temperature (35, 37 and 39 degrees C) or the oxygen content (16, 21 and 26%) had little effect on either the growth of cells in the presence of gamma-linolenic acid or on thiobarbiturate reactive material levels over a 7 day period. Thus, small changes in cell culture conditions do not affect 18:3n-6 cytotoxicity or markers of lipid peroxidation.
Cantrill
R C
RC
EFAMOL Research Institute, Kentville, Nova Scotia, Canada.
Ells
G W
GW
Chisholm
K A
KA
Hughes
B J
BJ
Horrobin
D F
DF
D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D016923:Cell Death; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005453:Fluorescence; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation; D010100:Oxygen / Q000008:administration & dosage*; D013696:Temperature; D013858:Thiobarbiturates / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D008041:Linoleic Acids; D013858:Thiobarbiturates; D019787:Linoleic Acid; C029684:thiobarbituric acid; D010100:Oxygen