Singly-linked catenation and knotting of cisplatin-DNA adduct by DNA topoisomerase I.
We studied the topological interaction between topoisomerase I (topo I) and cisplatin-DNA adduct. The topoisomers of the cisplatinized DNA formed by topo I are different in none-, low- and high-cisplatin concentrations to bind to DNA. Two unique topologically distinct invariants, singly-linked catenane and torefoiled knot DNA generated from cisplatin adduct, by topological reaction but not from none cisplatinized DNA. The results are discussed in relation with a possible recombinational role of cisplatin on the topological reaction with DNA.
Kobayashi
S
S
Department of Drug Analysis, Showa College of Pharmaceutical Science, Tokyo, Japan.
Furukawa
M
M
Hamajima
H
H
Arai
T
T
Ishii
Y
Y
Tobinaga
S
S
Tanaka
A
A
D002945:Cisplatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D018736:DNA Adducts; D004264:DNA Topoisomerases, Type I / Q000737:chemistry*; D008854:Microscopy, Electron; D008968:Molecular Conformation
D018736:DNA Adducts; C057270:cisplatin-DNA adduct; D004247:DNA; D004264:DNA Topoisomerases, Type I; D002945:Cisplatin