Identification of an endogenous mammary tumor virus involved in the clonal deletion of V beta 2 T cells.
Expression of V beta (beta-chain variable region) gene segments was investigated in the Mus m. domesticus DDO strain, which possesses a large genomic deletion encompassing 20 of the 29 V beta gene segments known in BALB/c. Stainings using V beta-specific monoclonal antibodies revealed that up to 60% of the peripheral T cells use 3 V beta gene segments. Variable frequencies of V beta 2 T cells were observed among DDO individuals. Segregation analyses of F2 crosses between V beta 2-deletor mice and mammary tumor virus (Mtv)-free mice led to the identification of a new endogenous Mtv, named Mtv-DDO, mediating V beta 2 T cell clonal deletion. Mtv-DDO structure is conserved with the exception of the carboxy-terminal region as compared to other Mtv. Comparison between Mtv sharing the same V beta specificity and isolated from laboratory or wild mice confirms that a stretch of 11 amino acids, defined as the V beta-specific region, is required for the V beta-specific interaction. Limited substitutions in this region account for the shift of the Mtv specificity towards different V beta.
Jouvin-Marche
E
E
Département d'Immunologie Institut Pasteur, Paris, France.
Marche
P N
PN
Six
A
A
Liebe-Gris
C
C
Voegtle
D
D
Cazenave
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002999:Clone Cells / Q000276:immunology / Q000382:microbiology; D004279:DNA, Viral / Q000235:genetics; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D018089:Superantigens / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; D014764:Viral Proteins