Sequencing using pulsed field and image reconstruction.
The use of pulsed fields in a standard manual sequencing set-up results in the separation of > 2 kb on a single gel, as compared to 300-400 bases with a dc field. However, visual reading of the sequence from a film exposed to a pulsed-field gel is not possible for more than 800-900 bases under the best conditions. The use of image reconstruction and enhancement techniques allows the reading of the M13mp18 sequence to > 1 kb, and individual bands can be identified at > 2 kb.
Noolandi
J
J
Xerox Research Centre of Canada, Mississauga, Ontario.
Forsyth
R
R
Shi
A C
AC
D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D005821:Genetic Techniques; D007202:Indicators and Reagents; D008433:Mathematics; D008969:Molecular Sequence Data; D011336:Probability; D013698:Templates, Genetic
D004279:DNA, Viral; D007202:Indicators and Reagents; D004247:DNA