Molecular analysis of the leukotoxin determinants from Pasteurella haemolytica serotypes 1 to 16.
All sixteen serotypes of Pasteurella haemolytica were shown to produce a leukotoxin protein which is immunologically related to the well-characterized serotype 1 leukotoxin. All of the leukotoxins were weakly hemolytic and were able to bind to BL-3 target cells. The leukotoxin determinants were characterized by Southern blot hybridization by use of the previously cloned serotype 1 determinant as the probe, and a number of distinct classes were identified. The leukotoxin determinants from serotypes 2, 3, and 11 were cloned. Nucleotide sequence analysis of the lktC and lktA genes of the serotype 3 and 11 determinants revealed nucleotide substitutions throughout the coding sequences. A comparison of the lktC and lktA genes and deduced proteins of serotypes 1, 3, and 11 showed that they are highly homologous.
Burrows
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Department of Microbiology, University of Guelph, Ontario, Canada.
Olah-Winfield
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D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005098:Exotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D016978:Mannheimia haemolytica / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D014774:Virulence / Q000235:genetics
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; C018579:leukotoxin