title: The Arabidopsis endoplasmic reticulum retention receptor functions in yeast.
abstract: Soluble proteins retained in the lumen of the endoplasmic reticulum (ER) contain a carboxyl-terminal tetrapeptide sequence that functions presumably to recycle these proteins from a subsequent compartment. Biochemical and genetic evidence indicate that the ERD2 gene product is the receptor for these ER retention signals. Here we report the identification of a cDNA clone from Arabidopsis thaliana (aERD2) similar in sequence and size to members of the ERD2 gene family. Southern and Northern blot analyses indicate that Arabidopsis contains a single aERD2 gene which is expressed at different levels in various plant tissues. A functional assay demonstrates that the Arabidopsis homologue, unlike the mammalian protein, can complement the lethal phenotype of the erd2 deletion mutant of Saccharomyces cerevisiae, indicating that this protein may have a similar function in plants. As the plant protein may have a binding specificity similar to the human Erd2 protein but can function in yeast, we suggest that the plant homologue is the functional link between yeast and animals.
authors.affiliation: Michigan State University-Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing 48824-1312.
authors.forename: H I
authors.initials: HI
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Gal
authors.forename: T C
authors.initials: TC
authors.lastname: Newman
authors.forename: N V
authors.initials: NV
authors.lastname: Raikhel
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000502:physiology*; D001692:Biological Transport; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000502:physiology*; D015870:Gene Expression; D017343:Genes, Plant; D005816:Genetic Complementation Test; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D018000:Receptors, Peptide; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; C064922:ERD2 protein, S cerevisiae; C489304:KDELR1 protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger; D018000:Receptors, Peptide; D029701:Saccharomyces cerevisiae Proteins