Deregulated c-fos modulates IgG2b production of B cells mediated by lipopolysaccharide.
We have examined effects of the deregulated c-fos protein on IgG2b production of B cells cultured with lipopolysaccharide (LPS) using splenic B cells from a transgenic line carrying the mouse c-fos gene under the control of the interferon alpha/beta (IFN) inducible Mx promoter (Mx-c-fosD). High c-fos expression was induced in the Mx-c-fosD B cells during the first two days of culture. DNA synthesis and IgG2b production were augmented in the culture. When IFN was added together with LPS, the high c-fos expression was prolonged until day 3 of culture. IgG2b production was remarkably suppressed. However, the production was not suppressed by upregulation of c-fos via exogenous IFN on day 4 of culture. These results suggest a regulatory effect of the c-fos protein on the differentiation of B cells to IgG2b producing cells at a distinct period.
Takada
M
M
Department of Immunology, ICMR, Kobe University School of Medicine, Japan.
Koizumi
T
T
Bachiller
D
D
Rüther
U
U
Tokuhisa
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D016762:Genes, fos / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000145:classification; D007370:Interferon Type I / Q000276:immunology; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000276:immunology*; D015854:Up-Regulation
D007074:Immunoglobulin G; D007370:Interferon Type I; D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos; D004247:DNA