Probes for the study of mupirocin resistance in staphylococci.
Probes constructed from a 4.05-kb EcoRI digest fragment of a mupirocin resistance plasmid and a 751-bp internal part of this fragment hybridised with DNA from all of 36 independent high-level mupirocin-resistant staphylococci tested from seven centres; most were Staphylococcus aureus. In most instances the probes detected an EcoRI digest fragment of approximately 4 kb. Probes did not hybridise to DNA from low-level resistant strains, nor from strains sensitive to mupirocin.
Rahman
M
M
Department of Microbial Diseases, St John's Institute of Dermatology, St Thomas' Hospital, London.
Noble
W C
WC
Dyke
K G
KG
D015342:DNA Probes; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D016712:Mupirocin / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D011815:R Factors; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics
D015342:DNA Probes; D016712:Mupirocin