title: A simple protocol for the physical cleavage of Trypanosoma cruzi kinetoplast DNA present in blood samples and its use in polymerase chain reaction (PCR)-based diagnosis of chronic Chagas disease.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
authors.forename: C
authors.initials: C
authors.lastname: Britto
authors.forename: M A
authors.initials: MA
authors.lastname: Cardoso
authors.forename: P
authors.initials: P
authors.lastname: Wincker
authors.forename: C M
authors.initials: CM
authors.lastname: Morel
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000175:diagnosis*; D018105:DNA, Kinetoplast / Q000032:analysis*; D006358:Hot Temperature; D016133:Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D018105:DNA, Kinetoplast