title: Generation of matrix-degrading proteolytic system from fibronectin by cathepsins B, G, H and L.
abstract: By their endoproteinase activities, cathepsins B, G, H and L can generate matrix-degrading proteolytic system from fibronectin. All four cathepsins studied cleaved fibronectin in fragments that were either proteolytically active or activated after incubation at pH 7.4 and in the presence of Ca2+. The highest enhancement of the matrix protein-degrading activity was observed after a gelatin-affinity chromatography of each digest. These results suggest that the effect of cathepsins at physiological pH in vivo may be enhanced by the activation of a matrix-degrading proteolytic system from fibronectin.
authors.affiliation: Laboratorie de Chimie des Protéines, Institut Pasteur, Paris, France.
authors.forename: X
authors.initials: X
authors.lastname: Blondeau
authors.forename: S L
authors.initials: SL
authors.lastname: Vidmar
authors.forename: I
authors.initials: I
authors.lastname: Emod
authors.forename: M
authors.initials: M
authors.lastname: Pagano
authors.forename: V
authors.initials: V
authors.lastname: Turk
authors.forename: V
authors.initials: V
authors.lastname: Keil-Dlouha
mesh_terms: D002403:Cathepsins / Q000494:pharmacology*; D002846:Chromatography, Affinity; D010450:Endopeptidases / Q000378:metabolism; D004789:Enzyme Activation; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D005780:Gelatin; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D005780:Gelatin; D002403:Cathepsins; D010450:Endopeptidases; D010447:Peptide Hydrolases