title: Serum antibody opsonic activity against Actinobacillus actinomycetemcomitans in human periodontal diseases.
abstract: Actinobacillus actinomycetemcomitans is frequently associated with severe periodontitis. Many periodontitis patients have elevated levels of serum IgG antibodies to A. actinomycetemcomitans, but the role of these antibodies is unknown. This study evaluated the functional capacity of anti-A. actinomycetemcomitans IgG antibody to enhance phagocytosis of A. actinomycetemcomitans by polymorphonuclear leukocytes. Chemoluminescence assays were done using sera from 64 subjects, 61 of whom had severe periodontitis; results were compared with the subject's anti-A. actinomycetemcomitans IgG titer and avidity. There was a strong correlation between chemoluminescence and antibody log titer (P < .00001) and a weak correlation between chemoluminescence and antibody avidity (P < .05). The results support the hypothesis that anti-A. actinomycetemcomitans IgG antibodies are important in promoting phagocytosis and killing of A. actinomycetemcomitans. Subjects who develop high levels of highly avid antibodies against A. actinomycetemcomitans may have greater resistance to continued or repeated infection by this pathogen.
authors.affiliation: Dept. of Periodontics, University of Washington, Seattle 98195.
authors.forename: K
authors.initials: K
authors.lastname: Underwood
authors.forename: K
authors.initials: K
authors.lastname: Sjöström
authors.forename: R
authors.initials: R
authors.lastname: Darveau
authors.forename: R
authors.initials: R
authors.lastname: Lamont
authors.forename: H
authors.initials: H
authors.lastname: Schenkein
authors.forename: J
authors.initials: J
authors.lastname: Gunsolley
authors.forename: R
authors.initials: R
authors.lastname: Page
authors.forename: D
authors.initials: D
authors.lastname: Engel
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology* / Q000382:microbiology; D000293:Adolescent; D000328:Adult; D000368:Aged; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D002648:Child; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009895:Opsonin Proteins / Q000097:blood / Q000276:immunology*; D010510:Periodontal Diseases / Q000097:blood / Q000276:immunology* / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D009895:Opsonin Proteins