title: Antigenic analysis of Leishmania isolates from Tachira state, Venezuela.
abstract: Studies of cutaneous leishmaniasis in 3 endemic foci in Tachira state, western Venezuela have revealed sympatric populations of parasites causing both cutaneous and mucocutaneous disease. Immunological techniques and measurement of protease/acid phosphatase activities have been used to detect species-specific parasite antigens from 3 isolates from Tachira. Identified antigens of particular interest had molecular masses of 100, 82, 66, 50 and 27 kDa, but there was a high degree of heterogeneity between the antigens of the Tachira isolates and other Venezuelan strains of Leishmania braziliensis and L. mexicana. This heterogeneity has implications concerning the selection of antigens for use in serodiagnosis of leishmaniasis.
authors.affiliation: Liverpool School of Tropical Medicine, Merseyside, UK.
authors.forename: R E
authors.initials: RE
authors.lastname: Adamson
authors.forename: A G
authors.initials: AG
authors.lastname: Hernandez
authors.forename: M L
authors.initials: ML
authors.lastname: Chance
authors.forename: R
authors.initials: R
authors.lastname: Bonfante-Garrido
authors.forename: R D
authors.initials: RD
authors.lastname: Maingon
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000737:chemistry; D015153:Blotting, Western; D006801:Humans; D007891:Leishmania / Q000737:chemistry / Q000276:immunology*; D007892:Leishmania braziliensis / Q000276:immunology; D007894:Leishmania mexicana / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D013045:Species Specificity
chemical_list: D000953:Antigens, Protozoan; D000135:Acid Phosphatase; D010447:Peptide Hydrolases