A simplified method for typing haemoglobin using ultrathin-layer isoelectric focusing.
Haemoglobin alleles were identified by isoelectric focusing using ultrathin-layer polyacrylamide gels, containing pH 3.0-10.0 ampholytes. The run parameters were chosen to co-ordinate with other isoelectric focusing methods currently used by the authors' laboratory. The method devised separated the A, F, S, and C alleles.
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Office of the Chief Medical Examiner, Department of Forensic Biology, New York, NY 10016.
Shenouda
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de Castro
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D000755:Anemia, Sickle Cell / Q000097:blood; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D011180:Postmortem Changes
D006454:Hemoglobins