ADP-ribosylation of Rho proteins inhibits sperm motility.
The highly homologous Rho proteins RhoA, RhoB and RhoC are low-molecular-mass GTP-binding proteins. They are selectively ADP-ribosylated by Clostridium botulinum ADP-ribosyltransferase C3 (C3 exoenzyme). The biological function of the Rho proteins is still unclear; there is evidence that they are involved in the regulation of the filamental network of cells. Here we report that C3 exoenzyme-like toxins ADP-ribosylate small GTP-binding proteins in bovine spermatozoa and inhibit sperm motility. These findings indicate that Rho proteins which reportedly regulate the microfilament system are basically involved in sperm motility.
Hinsch
K D
KD
Zentrum für Dermatologie und Andrologie, Justus-Liebig-Universität Giessen, Germany.
Habermann
B
B
Just
I
I
Hinsch
E
E
Pfisterer
S
S
Schill
W B
WB
Aktories
K
K
D036002:ADP Ribose Transferases / Q000378:metabolism*; D000818:Animals; D001905:Botulinum Toxins; D002417:Cattle; D003014:Clostridium botulinum / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D013081:Sperm Motility; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; D020743:rhoB GTP-Binding Protein
D008565:Membrane Proteins; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; D020743:rhoB GTP-Binding Protein