title: Cloning and characterization of the rat 5-HT5B receptor. Evidence that the 5-HT5B receptor couples to a G protein in mammalian cell membranes.
abstract: A gene encoding a novel G protein-coupled 5-hydroxytryptamine (5-HT) receptor, termed 5-HT5B, was cloned. The ligand binding profile of this receptor is distinct from that of other cloned 5-HT receptors. The 5-HT5B receptor couples to a G protein in COS1 cell membranes; however, activation of the 5-HT5B receptor does not appear to alter either cAMP accumulation or phosphoinositide turnover in a variety of fibroblast cell lines. In the rat brain, 5-HT5B gene expression occurs predominantly in the medial habenulae and hippocampal CA1 cells of the adult. Little expression is seen during embryonic development.
authors.affiliation: Center for Molecular Biology, University of Heidelberg ZMBH, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Wisden
authors.forename: E M
authors.initials: EM
authors.lastname: Parker
authors.forename: C D
authors.initials: CD
authors.lastname: Mahle
authors.forename: D A
authors.initials: DA
authors.lastname: Grisel
authors.forename: H P
authors.initials: HP
authors.lastname: Nowak
authors.forename: F D
authors.initials: FD
authors.lastname: Yocca
authors.forename: C C
authors.initials: CC
authors.lastname: Felder
authors.forename: P H
authors.initials: PH
authors.lastname: Seeburg
authors.forename: M M
authors.initials: MM
authors.lastname: Voigt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D004622:Embryo, Mammalian / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D051381:Rats; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D011985:Receptors, Serotonin; C078730:serotonin 5 receptor; D004247:DNA; D019204:GTP-Binding Proteins