Partial hepatectomy of rats 3 weeks before or simultaneously with 2-aminofluorene injection can affect the amounts of adducts induced in hepatic DNA.
The influence of partial hepatectomy on the level of 2-aminofluorene (2-AF) induced DNA adducts in rat liver was studied. We found that partial hepatectomy performed either 3 weeks before or simultaneously with the injection of 2-AF affected the amounts of adducts in rat hepatic DNA compared to controls. The level of DNA adducts in rats that were treated with 2-AF and simultaneously hepatectomized was higher (19.9 fmol/microgram DNA) than in non-hepatectomized ones (14.4 fmol/microgram DNA) when measured 48 h after 2-AF administration. In rats treated with the carcinogen 3 weeks after hepatectomy the level of DNA adducts was significantly higher than in nonhepatectomized rats when measured 15 days after the injection of 2-AF (10.9 fmol/microgram DNA and 5.9 fmol/microgram DNA respectively). The high level of DNA adducts in that group of hepatectomized animals was correlated with a relatively lower rate of 2-AF-induced radioactive thymidine incorporation into hepatic DNA (in comparison to non-hepatectomized rats).
Widłak
P
P
Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
Widłak
W
W
Rzeszowska-Wolny
J
J
D000818:Animals; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D005449:Fluorenes / Q000378:metabolism* / Q000633:toxicity*; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013936:Thymidine / Q000378:metabolism
D002273:Carcinogens; D005449:Fluorenes; C012177:2-aminofluorene; D004247:DNA; D013936:Thymidine