title: Aflatoxin B1-binding proteins in primary cultured hepatocytes of chicken embryo: studies in vivo and in vitro.
abstract: Among several aflatoxin B1 (AFB1)-binding proteins in primary cultured hepatocytes of chicken embryo, a cytosolic 25 kDa protein proved to be an AFB1-specific binding protein. Binding of AFB1 to a cytosolic protein of the same molecular mass as that seen in vivo was also detectable in a cell-free system and was dependent on the microsomal components. The binding was observed in the in vitro systems derived from AFB1-sensitive animals such as chicken, rabbit, and rat. These findings suggest that the 25 kDa AFB1-binding protein(s) is involved in the intracellular process of AFB1-mediated toxicity.
authors.affiliation: Department of Biomedical Food Research, National Institute of Health, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Iwaki
authors.forename: S
authors.initials: S
authors.lastname: Kumagai
authors.forename: Y
authors.initials: Y
authors.lastname: Akamatsu
authors.forename: T
authors.initials: T
authors.lastname: Kitagawa
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000633:toxicity; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002474:Cell-Free System; D002478:Cells, Cultured; D002642:Chick Embryo; D003600:Cytosol / Q000737:chemistry; D008099:Liver / Q000737:chemistry* / Q000196:embryology; D011485:Protein Binding; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D016604:Aflatoxin B1