Cross-reactive response to human immunodeficiency virus type 1 (HIV-1) gp120 and HLA class I heavy chains induced by receipt of HIV-1-derived envelope vaccines.
Autoantibodies specific to HLA class I antigens were detected in the sera of persons vaccinated with human immunodeficiency virus type 1-derived recombinant vaccines by using synthetic peptides representing the amino acid sequences recognized by an HLA class I/gp120 cross-reactive monoclonal antibody. Study subjects received recombinant vaccinia gp160 (vacc-env) alone, vacc-env followed by one dose of recombinant gp160 (rgp160, 640 micrograms), or four doses of rgp160 alone (640 or 80 micrograms). All sera from vacc-env/rgp160-vaccinated subjects contained HLA/gp120 cross-reactive antibodies, as did all sera from recipients of the rgp160 alone at 640 micrograms/dose. In contrast, none of the sera from subjects who received either the vacc-env alone or the 80 micrograms/dose rgp160 alone contained detectable HLA cross-reactive antibodies, and these same sera showed little or no envelope reactivity on Western blot. The results showed a striking correlation between immunogenicity and the induction of cross-reactive antibodies by the rgp160 vaccine.
De Santis
C
C
Department of Biological and Technological Research, Ospedale S. Raffaele, Milan, Italy.
Robbioni
P
P
Longhi
R
R
Lopalco
L
L
Siccardi
A G
AG
Beretta
A
A
Roberts
N J
NJ
D016915:AIDS Vaccines / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D005260:Female; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D014614:Vaccines, Synthetic / Q000276:immunology
D016915:AIDS Vaccines; D001323:Autoantibodies; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D015395:Histocompatibility Antigens Class I; D014614:Vaccines, Synthetic