Antioxidant and radical scavenging effects of anthraquinones and anthrones.
The activity of seven anthraquinones and four anthrones against nonenzymatic and enzymatic lipid peroxidation in vitro and their ability to scavenge free radicals have been studied. In nonenzymatic peroxidation in rat hepatocytes induced by t-butyl hydroperoxide, dithranol and anthrone were the strongest antioxidants, having IC50 values of 8 +/- 1 and 24 +/- 5 mumol/l, respectively. Rhein (IC50 64 +/- 2 mumol/l) and aloe-emodin (IC50 65 +/- 3 mumol/l) showed the highest inhibitory activity against peroxidation of linoleic acid catalyzed by soybean 15-lipoxygenase. Anthrone (IC50 62 +/- 2 mumol/l), dithranol (IC50 72 +/- 1 mumol/l) and rhein anthrone (IC50 76 +/- 6 mumol/l) were the most effective radical scavengers of the diphenylpicrylhydrazyl radical. The antioxidant activities in hepatocytes and the radical scavenging activities were correlated, whereas the inhibition of enzymatic lipid peroxidation showed no correlation with the two other effects.
Malterud
K E
KE
Department of Pharmacognosy, University of Oslo, Norway.
Farbrot
T L
TL
Huse
A E
AE
Sund
R B
RB
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology*; D000880:Anthraquinones / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D015764:Bepridil / Q000031:analogs & derivatives; D001713:Biphenyl Compounds; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers; D005609:Free Radicals; D015227:Lipid Peroxidation / Q000187:drug effects; D016859:Lipoxygenase Inhibitors; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010545:Peroxides; D010851:Picrates; D051381:Rats; D017208:Rats, Wistar; D020122:tert-Butylhydroperoxide; D000095322:Anthrones
D000873:Anthracenes; D000880:Anthraquinones; D000975:Antioxidants; D015764:Bepridil; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D016859:Lipoxygenase Inhibitors; D010545:Peroxides; D010851:Picrates; D020122:tert-Butylhydroperoxide; C004931:1,1-diphenyl-2-picrylhydrazyl; C004522:anthrone; D000095322:Anthrones