RPII15 codes for the M(r) 15,000 subunit 9 of Drosophila melanogaster RNA polymerase II.
The RPII15 gene product of Drosophila melanogaster, which has recently been identified by sequence comparison, possesses a high similarity to subunit 9 of yeast RNA polymerase II. Using the polymerase chain reaction the coding region of RPII15 was isolated from genomic DNA of adult flies. Sequence analysis shows four amino acid substitutions in comparison to the previously reported sequence. Antisera were generated against bacterially expressed RPII15 and were used for immunoblotting experiments with RNA polymerase II of Drosophila melanogaster. This analysis identified the M(r) 15,000 subunit 9 as gene product of RPII15.
Liu
Z
Z
Institute of Molecular Genetics, University of Heidelberg, Germany.
Kontermann
R E
RE
Schulze
R A
RA
Petersen
G
G
Bautz
E K
EK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D015151:Immunoblotting; D058619:Maltose-Binding Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism
D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D011993:Recombinant Fusion Proteins; D012319:RNA Polymerase II