Loop-like structure of dGTTG in solution.
A strong NOESY cross peak was observed between G1H8 and G4H8 for a single-stranded tetradeoxyoligonucleotide dGTTG in aqueous solution. If dGTTG is in an expanded form, NOE should not be observed because the distance between G1H8 and G4H8 is estimated to be about 13 A. This NOESY cross peak shows that the guanine residues of the both ends of dGTTG exist with short distance (< 5 A), and that dGTTG has a distorted loop-like structure in aqueous solution. This is the shortest single-stranded oligodeoxynucleotide that has a rigid structure in solution.
Makino
K
K
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Nishi
M
M
Suzuki
T
T
Ohsumi
S
S
Morii
T
T
Ide
H
H
Murakami
A
A
D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012996:Solutions
D009838:Oligodeoxyribonucleotides; D012996:Solutions