title: Effect of aflatoxin-contaminated feed on morbidity and residues in walleye fish.
abstract: The effect of aflatoxin in the feed on the morbidity and toxin residue in the musculature of walleye fish was studied. The fish were divided into 3 groups of 12 each; group 1 served as controls, groups 2 and 3 received feed treated with 50 or 100 ppb toxin for 30 d respectively. Fish were kept in glass aquaria, 4 fish/aquarium, with aerator and solids-waste filter system. Fish were observed for morbidity and mortality. Six fish from each group were sacrificed after 30 d and examined for gross pathological lesions and histopathological changes. In addition, fish musculature were analyzed for aflatoxin. One out of 16 (8%) of the fish fed 100 ppb aflatoxin in 48 h. Pale livers and significant degenerative changes were observed in both toxin-treated groups. Aflatoxin B1, G1 and G2 were detected in the musculature of both groups at a concentration of 5, 10, 15, 20 ppb respectively. After 2 w of aflatoxin withdrawal, no aflatoxin was detectable in the fish muscle. However, marked histopathological lesions were still observed.
authors.affiliation: Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.
authors.forename: M
authors.initials: M
authors.lastname: Hussain
authors.forename: M A
authors.initials: MA
authors.lastname: Gabal
authors.forename: T
authors.initials: T
authors.lastname: Wilson
authors.forename: R C
authors.initials: RC
authors.lastname: Summerfelt
mesh_terms: D000348:Aflatoxins / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D005399:Fishes; D005506:Food Contamination; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D009132:Muscles / Q000737:chemistry*
chemical_list: D000348:Aflatoxins