[Effects of anisodamine and ampicillin on cerebrospinal fluid in experimental E coli meningitis].
24 hr and 27 hr after K1-positive E coli suspended in 0.25 ml of saline (E coli 8 x 10(7)/ml) was injected into the cisterna magna of Chinese rabbits, the levels of bacterial titer, endotoxin, malondialdehyde (MDA), lactate and lactic dehydrogenase (LDH) of the cerebrospinal fluid (CSF) were increased significantly. After ampicillin therapy alone for 3 hr, the level of CSF bacterial titer was decreased and that of LDH increased, but the others didn't change significantly. After anisodamine and ampicillin therapy for 3 hr, the levels of CSF endotoxin and lactate concentrations were decreased from 3.2 +/- 0.6 to 2.1 +/- 0.9 log 10 IU/ml and from 8.6 +/- 2.0 to 6.5 +/- 1.8 mmol/L respectively. The CSF MDA concentration (0.31 +/- 0.07) was lower than that of the nontreatment group (0.59 +/- 0.25) or the ampicillin treatment group (0.59 +/- 0.21 nmol/ml) (P < 0.01 or 0.05). These results suggest that anisodamine combined with antibacterial therapy for experimental E coli meningitis is superior to antibacterial treatment in efficacy.
Liu
Y X
YX
Department of Pediatrics, Affiliated Xiangya Hospital, Hunan Medical University, Changsha.
D000667:Ampicillin / Q000008:administration & dosage*; D000818:Animals; D004359:Drug Therapy, Combination; D004731:Endotoxins / Q000134:cerebrospinal fluid; D004927:Escherichia coli Infections / Q000134:cerebrospinal fluid / Q000188:drug therapy*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000134:cerebrospinal fluid; D008297:Male; D008315:Malondialdehyde / Q000134:cerebrospinal fluid; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000188:drug therapy* / Q000382:microbiology; D011817:Rabbits; D012991:Solanaceous Alkaloids / Q000008:administration & dosage*; D014665:Vasodilator Agents / Q000008:administration & dosage*
D004731:Endotoxins; D012991:Solanaceous Alkaloids; D014665:Vasodilator Agents; C003922:anisodamine; D008315:Malondialdehyde; D000667:Ampicillin; D007770:L-Lactate Dehydrogenase