X-arrestin: a new retinal arrestin mapping to the X chromosome.
We have been using a differential cDNA cloning approach to isolate human retina-specific and retina-enriched genes [1]. A 1,314 bp cDNA was isolated by this approach, representing a highly retina-specific message encoding a 388 amino acid protein showing 58%, 50%, and 49% homology to bovine beta-arrestin, and bovine and human retinal arrestin (S-antigen), respectively. Chromosomal mapping localized this new arrestin gene to the proximal long arm of the X chromosome, hence it was named X-arrestin. In situ hybridization demonstrated its expression in the inner and outer segments and the inner plexiform regions of the retina.
Murakami
A
A
Laboratory of Molecular Genetics, Bascom Palmer Eye Institute, University of Miami School of Medicine, FL 33136.
Yajima
T
T
Sakuma
H
H
McLaren
M J
MJ
Inana
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000096:biosynthesis / Q000235:genetics*; D019393:Arrestin; D019390:Arrestins; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013961:Thyroid Gland / Q000378:metabolism; D014960:X Chromosome
D000941:Antigens; D019393:Arrestin; D019390:Arrestins; D018076:DNA, Complementary; D005136:Eye Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C082333:arrestin3