title: Identification of casein kinase II as a major endogeneous caldesmon kinase in sheep aorta smooth muscle.
abstract: A caldesmon kinase activity was detected in an ATP extract of the myofibril-like pellet from sheep aorta. The enzyme was purified 745-fold and was identified as casein kinase II on the basis of molecular size, substrate specificity, and high sensitivity to heparin inhibition. Casein kinase II phosphorylated isolated caldesmon and caldesmon incorporated into native thin filaments, and transferred about 1 mol of phosphate per mol of caldesmon-h. Ser-73 was the main site phosphorylated by casein kinase II in chicken gizzard caldesmon. Phosphorylation of caldesmon reduced its affinity for smooth muscle myosin but had no effect upon the ability of caldesmon to inhibit the ATPase activity of actomyosin.
authors.affiliation: Institute of Experimental Cardiology, Russian National Cardiology Centre, Moscow.
authors.forename: A V
authors.initials: AV
authors.lastname: Vorotnikov
authors.forename: N B
authors.initials: NB
authors.lastname: Gusev
authors.forename: S
authors.initials: S
authors.lastname: Hua
authors.forename: J H
authors.initials: JH
authors.lastname: Collins
authors.forename: C S
authors.initials: CS
authors.lastname: Redwood
authors.forename: S B
authors.initials: SB
authors.lastname: Marston
mesh_terms: D000818:Animals; D001011:Aorta; D047390:Casein Kinase II; D002645:Chickens; D006493:Heparin / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012756:Sheep; D013379:Substrate Specificity
chemical_list: D047428:Protein Kinase Inhibitors; D006493:Heparin; D011494:Protein Kinases; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; C056959:caldesmon kinase