Does phosphoglucoisomerase display anomeric specificity or selectivity towards alpha-D-glucose 6-phosphate? An assessment by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r.
The study by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r. of hexose 6-phosphates interconversion in the reaction catalysed by yeast phosphoglucoisomerase reveals that the enzyme displays anomeric selectivity, rather than specificity, towards alpha-D-glucose 6-phosphate. Indeed, beta-D-glucose 6-phosphate participates for about 20% to the total and direct conversion of the aldohexose into oxohexose ester.
Kayser
F
F
High Resolution NMR Centre, Faculty of Engineering, Free University of Brussels (VUB), Belgium.
Biesemans
M
M
Willem
R
R
Malaisse-Lagae
F
F
Malaisse
W J
WJ
D019298:Glucose-6-Phosphate; D005956:Glucose-6-Phosphate Isomerase / Q000737:chemistry / Q000378:metabolism*; D005958:Glucosephosphates / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D010759:Phosphorus Isotopes; D013379:Substrate Specificity
D005958:Glucosephosphates; D010759:Phosphorus Isotopes; D019298:Glucose-6-Phosphate; D005956:Glucose-6-Phosphate Isomerase