[The hierarchy of complexes and compact structures of trivaline with nucleic acids. I. Interaction of trivaline with double-stranded DNA].
It was shown that trivaline-poly(U) complexes have no appreciable fluorescence at any peptide concentration if the polymer concentration is less than critical. By electron microscopy it was shown that dsDNA molecules undergo a second step of compactization if polymer and peptide concentrations are high enough. The diameter of this rod-like particles is over 40 nm. If the polymer concentration is less than critical, dsDNA molecules form with trivaline extended structures of dissimilar morphology. We propose a scheme of trivaline-dsDNA complexes and compact structure formation, beginning from a dimer complex. It is consistent with the results of equilibrium dialysis, fluorescence, electron microscopy, flow linear and circular dichroism measurements.
Strel'tsov
S A
SA
Semenov
T E
TE
Moroz
O V
OV
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D005453:Fluorescence; D008854:Microscopy, Electron; D011072:Poly U / Q000737:chemistry*; D011487:Protein Conformation; D014633:Valine / Q000737:chemistry*
D011072:Poly U; D004247:DNA; D014633:Valine
Ob ierarkhii kompleksov i kompaktnykh struktur trivalina s nukleinovymi kislotami. I. Vzaimodeĭstvie trivalina s dtsDNK.