title: Substrate-bound GP130/F11 will promote neurite outgrowth: evidence for a cell surface receptor.
abstract: GP130/F11 is a neuronal cell adhesion molecule belonging to the Ig superfamily. Preliminary evidence suggests that it may be important for the fasciculation of axons and cell-cell interactions during synaptogenesis. Previous work has shown that when F3 (the mouse homologue) is transfected into Chinese hamster ovary (CHO) cells it will act as a substrate for neuronal growth and adhesion. In this study we have shown that isolated GP130/F11 will also act as a substrate for the growth of chick dorsal root ganglia cell neurons. The concentration of GP130/F11 on the surface of sympathetic neurons, grown for one day in culture was approximately 40 ng cm-2 which was comparable to the levels required to stimulate neurite outgrowth. Deglycosylation of GP130/F11 or partial denaturation by boiling did not abolish the activity. However, after treatment with dithiothreitol, GP130/F11 was no longer capable of stimulating neurite outgrowth. GP130/F11 will also stimulate neurite outgrowth from forebrain neurons from 7 to 9-day-old embryos. These do not express GP130/F11 until around day 10, providing further evidence that GP130/F11 interacts with an as yet unknown cell surface receptor.
authors.affiliation: Department of Human Anatomy and Cell Biology, University of Liverpool, United Kingdom.
authors.forename: G A
authors.initials: GA
authors.lastname: Clarke
authors.forename: C A
authors.initials: CA
authors.lastname: White
authors.forename: D J
authors.initials: DJ
authors.lastname: Moss
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis* / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D058966:Contactins; D006023:Glycoproteins / Q000502:physiology*; D008024:Ligands; D015394:Molecular Structure; D019006:Neural Cell Adhesion Molecules; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000648:ultrastructure; D016548:Prosencephalon / Q000648:ultrastructure; D013564:Sympathetic Nervous System / Q000648:ultrastructure
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D058966:Contactins; D006023:Glycoproteins; D008024:Ligands; D019006:Neural Cell Adhesion Molecules