Resistance of lambda cI translation to antibiotics that inhibit translation initiation.
The lambda cI lysogenic transcript is unusual in having no leader. Expression of a cI-lacZ protein fusion was relatively resistant to kasugamycin and pactamycin, which inhibit translation initiation on transcripts with leaders. Our data imply that there are distinct differences in translation initiation between the two classes of transcripts.
Chin
K
K
Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Shean
C S
CS
Gottesman
M E
ME
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics*; D004268:DNA-Binding Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D008242:Lysogeny; D010142:Pactamycin / Q000633:toxicity*; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D001616:beta-Galactosidase / Q000096:biosynthesis
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004268:DNA-Binding Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D010142:Pactamycin; D001616:beta-Galactosidase; C100305:kasugamycin