title: Reduced binding capacity of concanavalin A-sepharose after treatment with chaotropic agents.
abstract: Affinity chromatography on Concanavalin A-Sepharose is widely used for purification of glycoproteins carrying mannose and glucose oligosaccharide moieties. High concentrations of chaotropes elute Concanavalin A from the gel matrix and significantly reduce the binding capacity of the column.
authors.affiliation: F. Hoffmann-La Roche Ltd., Pharmaceutical Research-New Technologies, Department of Biology, Basel, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Fountoulakis
authors.forename: J F
authors.initials: JF
authors.lastname: Juranville
mesh_terms: D000818:Animals; D002021:Buffers; D002846:Chromatography, Affinity / Q000379:methods; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D017830:Octoxynol / Q000494:pharmacology; D011489:Protein Denaturation; D012685:Sepharose / Q000031:analogs & derivatives* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
chemical_list: D002021:Buffers; D006146:Guanidines; C028326:concanavalin A-sepharose; D012967:Sodium Dodecyl Sulfate; D007371:Interferon-gamma; D014508:Urea; D017830:Octoxynol; D012685:Sepharose; D019791:Guanidine