title: Predicting the point at which transmembrane helices protrude from the bilayer: a model of the antenna complexes from photosynthetic bacteria.
abstract: We describe a method for predicting the point at which a transmembrane helix leaves the bilayer and enters the more polar region of the aqueous exterior. This is achieved by comparing the relative directions of the hydrophobic and internal faces of the transmembrane helices which should be opposite for the regions within the bilayer but equivalent for the regions on the outside. This information provides a strong constraint in the process of modelling membrane proteins. We go on to use the approach to model the monomers of the bacterial light-harvesting antenna complexes. This information is then combined with some preliminary crystallographic data and biochemical results to produce a 3-D model of a tetramer.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Leeds, UK.
authors.forename: D
authors.initials: D
authors.lastname: Donnelly
authors.forename: R J
authors.initials: RJ
authors.lastname: Cogdell
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005583:Fourier Analysis; D045342:Light-Harvesting Protein Complexes; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012241:Rhodopseudomonas / Q000737:chemistry*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: C081521:B800-850 light-harvesting complex, bacteria; D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D008051:Lipid Bilayers; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins