Inhibition of in vitro mineralization in osteoblastic cells and in mouse tooth germ by phosphatidylinositol-specific phospholipase C.
We have examined the effect of phosphatidylinositol-specific phospholipase C (PIPLC) on the in vitro mineralization during cultivation of both osteoblast-like MC3T3-E1 cells and the lower molar tooth germ from mouse embryo. PIPLC not only caused the release of alkaline phosphatase (ALP) from cell membranes into the culture medium but also produced a disturbance of in vitro mineralization in MC3T3-E1 cells and in mouse embryonic tooth germ. These findings strongly suggest that ALP, functioning as an ectoenzyme, is involved in the physiological mineralization process of both bone and teeth.
Togari
A
A
Department of Pharmacology School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Arakawa
S
S
Arai
M
M
Matsumoto
S
S
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000032:analysis; D002460:Cell Line / Q000187:drug effects; D066298:In Vitro Techniques; D051379:Mice / Q000196:embryology; D008903:Minerals / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism* / Q000494:pharmacology; D014083:Tooth Germ / Q000187:drug effects / Q000196:embryology*
D008903:Minerals; D010716:Phosphatidylinositols; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D002118:Calcium