[Plasmid pSVc-myc-1 induces gene mutations and chromosome aberrations in cultured Chinese hamster cells].
The induction of mutations to 6-thioguanine-resistance and chromosome aberrations by the plasmid pSVc-myc-1, carrying the activated cellular oncogene c-myc, isolated from a mouse plasmocytoma was studied in a cultured Chinese hamster cell line. The yield of HPRT- mutants and chromosome aberrations increased 1.6 times on the average after pSVc-myc-1 treatment. The mutagenic activity of pSVc-myc-1 was statistically significant. The role of mutagenic effects of activated cellular oncogenes in malignant transformations is discussed.
Bobrysheva
I V
IV
Baron
E M
EM
Varshaver
N B
NB
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects; D002869:Chromosome Aberrations / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D004351:Drug Resistance / Q000235:genetics; D016259:Genes, myc / Q000235:genetics*; D051379:Mice; D016296:Mutagenesis / Q000235:genetics*; D010954:Plasmacytoma / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D013866:Thioguanine / Q000037:antagonists & inhibitors; D014162:Transfection / Q000235:genetics
D013866:Thioguanine
Plazmidnye pSVc-myc-1 indutsiruet gennye mutatsii i khromosomnye aberratsii v kul'tiviruemykh kletkakh kitaĭskogo khomiachka.