title: Antibacterial and haemolytic peptides containing D-alloisoleucine from the skin of Bombina variegata.
abstract: A family of bombinin-related peptides is present in the skin of Bombina variegata. These peptides contain 27 residues with Gly as N-terminus and display antimicrobial activity. From sequence analysis of the cDNAs encoding for the corresponding peptide precursors, the presence of a novel 20-residue peptide with Ile as N-terminus was predicted. We have now purified a family of hydrophobic peptides named H1-H5, whose sequences correspond to the predicted peptide with some variability in positions 1, 2 and 8. In particular, H3-H5 contain a D-alloisoleucine residue in the second position. All these peptides display antibacterial and haemolytic activity.
authors.affiliation: Dipartimento di Scienze Biochimiche and Centro di Biologia, Università La Sapienza, Roma, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Mignogna
authors.forename: M
authors.initials: M
authors.lastname: Simmaco
authors.forename: G
authors.initials: G
authors.lastname: Kreil
authors.forename: D
authors.initials: D
authors.lastname: Barra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001001:Anura; D002851:Chromatography, High Pressure Liquid; D006460:Hemolysin Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006461:Hemolysis; D007532:Isoleucine / Q000032:analysis*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012867:Skin / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D006460:Hemolysin Proteins; D010455:Peptides; D007532:Isoleucine