title: Efficient loading of identical viral peptide onto class II molecules by antigenized immunoglobulin and influenza virus.
abstract: Several prior reports have identified peptides that are naturally associated with major histocompatibility complex (MHC) class II molecules on presenting cells. We have examined the delivery of a peptide from exogenous sources to MHC class II molecules. The peptide derives from the influenza virus hemagglutinin (HA) and activates a CD4+ T cell hybridoma. In functional assays of antigen presentation, this epitope is delivered effectively to T cells either in the context of influenza virus or chimeric immunoglobulin (Ig) molecules (Ig-HA) in which the peptide has replaced the CDR3 loop of the heavy chain. We find that the identical 11-mer peptide can be isolated from mouse MHC class II antigens whether the exogenous source of peptide is free HA peptide, the Ig-HA chimera, or ultraviolet-inactivated PR8 influenza virus. The Ig-HA chimera proves to be the most efficient vehicle for charging class II molecules via the exogenous route. Given the fact that self Igs represent natural long-lived carriers, we suggest that antigenized Igs have considerable potential for peptide delivery to MHC molecules in situ.
authors.affiliation: Department of Microbiology, Mount Sinai School of Medicine, New York 10029.
authors.forename: T D
authors.initials: TD
authors.lastname: Brumeanu
authors.forename: W J
authors.initials: WJ
authors.lastname: Swiggard
authors.forename: R M
authors.initials: RM
authors.lastname: Steinman
authors.forename: C A
authors.initials: CA
authors.lastname: Bona
authors.forename: H
authors.initials: H
authors.lastname: Zaghouani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000528:radiation effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D009980:Influenza A virus / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D016393:Lymphoma, B-Cell; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000949:Histocompatibility Antigens Class II; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins