Evidence of an intermediate-size beta-endorphin-containing precursor molecule in bovine pituitary by mass spectrometry.
A new analytical system has been designed to characterize intermediate-sized precursor molecules from two different precursors-the preproenkephalin Abovine,1-263 precursor molecule, which produces methionine enkephalin and from the proopiomelanocortin, POMCbovine,1-265 precursor molecule, which produces beta-endorphin. A possible intermediate-sized precursor that leads to beta-endorphin was found in bovine pituitary by means of electrospray ionization mass spectrometry, in combination with gel permeation chromatography, reversed-phase high performance liquid chromatography, radioimmunoassay, trypsinolysis, and liquid secondary ion mass spectrometry.
Yan
L
L
Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee, Memphis 38163.
Fridland
G
G
Tseng
J L
JL
Desiderio
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004745:Enkephalins / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010902:Pituitary Gland / Q000737:chemistry*; D011333:Pro-Opiomelanocortin / Q000737:chemistry / Q000302:isolation & purification*; D011498:Protein Precursors / Q000737:chemistry / Q000302:isolation & purification*; D014357:Trypsin; D001615:beta-Endorphin / Q000032:analysis* / Q000737:chemistry
D004745:Enkephalins; D011498:Protein Precursors; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin; C033660:preproenkephalin; D014357:Trypsin