[Antigenic changes of Tau protein induced by glutamate on primary cultures of neurons: immunocytochemistry study].
Degenerating neurons in Alzheimer's disease are characterized by the presence of neurofibrillary tangles constituted by paired helical filaments (PHF). Abnormally phosphorylated Tau protein, a microtubule associated protein is one of the major component of PHF. Abnormal phosphorylation seems to be located in the C-terminal domain but also in the N-terminal region of Tau proteins. Previous studies demonstrated that calcium-mediated glutamate toxicity produces a dose-dependent increase of Tau immunolabellings in neuronal cultures. Biochemical results revealed that these changes could be associated with abnormal Tau migrations on immunoblots. Using three anti-Tau antibodies the present study shows that glutamate toxicity induces in neuronal cultures, Tau modifications localized in the N- and C-terminal domains of the protein. These findings suggest the possibility that glutamate toxicity can induce Tau antigenic changes involving probably the whole molecule.
Sindou
P
P
Unité de Neurobiologie Cellulaire, Faculté de Médecine, Limoges.
Sautiere
P E
PE
Couratier
P
P
Delacourte
A
A
Hugon
J
J
D000818:Animals; D000940:Antigenic Variation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005971:Glutamates / Q000494:pharmacology*; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D016875:tau Proteins / Q000276:immunology*
D005971:Glutamates; D016875:tau Proteins
Modifications antigéniques de la protéine Tau induites par le glutamate sur des cultures primaires de neurones: études immunocytochemiques.