An ets-related gene, ERG, is rearranged in human myeloid leukemia with t(16;21) chromosomal translocation.
The t(16;21)(p11;q22) translocation is a nonrandom chromosomal abnormality found in several types of myeloid leukemia, which show variable cytomorphological features. We constructed rodent-human somatic cell hybrids containing the der(16) chromosome from leukemic cells of a patient with t(16;21). Using these hybrids, we mapped the translocation breakpoint on the Not I restriction map of chromosome 21 which we had previously constructed. The result showed the proximity of the breakpoint to the ERG gene, a member of the ets oncogene superfamily. Polymerase chain reaction and Southern blot analyses of genomic DNA from the hybrids and from peripheral blood cells and bone marrow cells of patients with t(16;21) showed that the breakpoints were clustered within a single intron in the coding region of the ERG gene. This finding and the results obtained by Northern blot analysis suggested the formation of a chimeric product(s) by fusion of the ERG gene and an unknown counterpart gene on chromosome 16.
Shimizu
K
K
Department of Immunology and Virology, Saitama Cancer Center Research Institute, Japan.
Ichikawa
H
H
Tojo
A
A
Kaneko
Y
Y
Maseki
N
N
Hayashi
Y
Y
Ohira
M
M
Asano
S
S
Ohki
M
M
D000208:Acute Disease; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001853:Bone Marrow / Q000473:pathology; D002885:Chromosomes, Human, Pair 16; D002891:Chromosomes, Human, Pair 21; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000302:isolation & purification; D015321:Gene Rearrangement; D006801:Humans; D006822:Hybrid Cells; D007951:Leukemia, Myeloid / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D012334:RNA, Neoplasm / Q000302:isolation & purification; D014157:Transcription Factors; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012334:RNA, Neoplasm; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases