The comparison of DNA synthesis inducibility by high potassium treatment in various nonproliferating cells.
Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root ganglia neurons and in vitro differentiated myotubes formed by L6 myoblasts. Murine peritoneal macrophages and macrophages/granulocytes derived from human promyelocytic leukemia cells HL60 are refractory to high potassium treatment. These results confirm the nonuniformity of the nonproliferative state of various differentiated cells.
Fedorov
Y V
YV
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Prudovsky
I A
IA
Yegorov
Y E
YE
Zelenin
A V
AV
D000818:Animals; D001345:Autoradiography; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D017737:Macrophages, Peritoneal / Q000166:cytology; D051379:Mice; D009132:Muscles / Q000166:cytology; D009432:Neural Crest / Q000166:cytology; D011188:Potassium / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D011188:Potassium