title: Cutaneous hypersensitivity responses in chickens divergently selected for antibody responses to sheep red blood cells.
abstract: Cellular immunity in vivo of chickens selected for high and low antibody responses to SRBC was evaluated by primary and secondary cutaneous hypersensitivity reactions to BSA and phytohemagglutinin (PHA)-induced delayed-type hypersensitivity (DTH). Similar to PHA-induced DTH, primary and secondary cutaneous hypersensitivity to BSA comprised acute (1- and 2-h), intermediate (4- and 6-h), and late (24-h) wing web swelling responses. Primary and secondary hypersensitivity to BSA revealed line differences between the High (H) line and a randombred Control (C) line on the one hand, and the Low (L) line on the other hand. Hypersensitivity to BSA was almost absent or decreased in the L line. The late phase of PHA-induced DTH was significantly elevated in the H line as compared with the L line. Naive recipients sensitized systematically with either serum from chickens sensitized with BSA, or with BSA-binding IgG, developed an antigen-specific early wing web swelling response following local challenge with BSA. It is suggested that the acute component of cutaneous hypersensitivity is mediated by Ig. The acute response may be a prerequisite for the development of classical (late) cutaneous DTH responses or may be initiated simultaneously with DTH. The current results indicate that the selection did not result in a higher hypersensitivity response to another T cell-dependent antigen and a mitogen in the H line, but in a lower response in the L line.
authors.affiliation: Department of Animal Husbandry, Agricultural University, Wageningen, The Netherlands.
authors.forename: H K
authors.initials: HK
authors.lastname: Parmentier
authors.forename: J W
authors.initials: JW
authors.lastname: Schrama
authors.forename: F
authors.initials: F
authors.lastname: Meijer
authors.forename: M G
authors.initials: MG
authors.lastname: Nieuwland
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000235:genetics*; D015153:Blotting, Western / Q000662:veterinary; D002352:Carrier Proteins / Q000097:blood; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006967:Hypersensitivity / Q000276:immunology / Q000662:veterinary; D010835:Phytohemagglutinins / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology; D012756:Sheep / Q000276:immunology; D012867:Skin / Q000276:immunology
chemical_list: D002352:Carrier Proteins; D010835:Phytohemagglutinins; D012710:Serum Albumin, Bovine