[Regulatory influence of Staphylococcal serine protease on selected functions of lung macrophages in vitro. Introduction of cellular carbonic paramagnetic probe method for evaluation of cellular phagocytic activity].
The study was aimed at evaluation of properties of serine protease produced by Staphylococcus aureus V8. Influence of enzyme on chemotactic and phagocytic activity of rat lung macrophages was investigated. Chemotactic activity of rat lung macrophages was studied by application of modified Boyden chambers. Slight induction of chemotaxis of cells preincubated with enzyme was found. Phagocytic activity of rat lung macrophages was investigated by application of Varian E3 spectrometer. EPR signals emitted by paramagnetic graphite phagocytized by macrophages were measured. Increased phagocytic activity of macrophages subjected to preincubation with enzyme in concentration of 10 micrograms/ml was observed. It seems that staphylococcal proteinase is engaged in interaction of staphylococci with immunological system cells.
Miedzobrodzki
J
J
Zakład Mikrobiologii i Immunologii, Uniwersytetu Jagiellońskiego, Krakowie.
Górka
M
M
Plonka
P
P
Turyna
B
B
D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D016676:Macrophages, Alveolar / Q000276:immunology*; D008297:Male; D010587:Phagocytosis / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000502:physiology*; D013210:Staphylococcus / Q000201:enzymology*
D012697:Serine Endopeptidases
Regulacyjny wpływ gronkowcowej proteinazy serynowej na wybrane funkcje makrofagów půcnych in vitro. Wprowadzenie metody komórkowej sondy paramagnetycznej weglowej dla oceny aktywnosci fagocytarnej komórek.