[A comparative study of pancreatic-duodenal transplantation, islets of Langerhans transplantation, and insulin treatment, in the control of experimental diabetes in the rat].
Sixty outbred Wistar rats were randomly assigned to five experimental groups: GI-10 non-diabetic control rats; GII-10 untreated diabetic control rats; GIII-10 diabetic rats treated with retard porcine insulin; GIV-20 diabetic rats that received pancreaticoduodenal transplantation (PDT) from normal donor rats; GV-10 diabetic rats submitted to islet of Langerhans transplantation (ILT) into the portal vein. The animals were housed in metabolic cages for six periods of 24 hours during 30 days and body weight, water and food intake, urine output, blood and urinary glucose were recorded. Diabetes was induced by I.V. administration of Alloxan (42 mg/kg of body weight); PDT was performed by microsurgical techniques and islets were prepared without enzymes. To prevent rejection. Cyclosporin A (10 mg/kg of body weight) was utilized in transplanted rats. PDT consistently and significantly (p < 0.05) improved the metabolic abnormalities of the diabetic rats, by restoring the body weight gain, and immediate relief of polydipsia, polyphagia, polyuria, hyperglycemia and glucosuria observed in pre-treatment period. PDT was more effective than ILT and this over insulin therapy on control of the diabetic state. However, the observed complications in GIV and GV, due to surgery and immunosuppression, should be analysed for the real benefits of the alternative therapy can be superior to eventual fails to the conventional therapy with insulin.
Breim
L C
LC
Laboratório de Técnica Cirúrgica e Cirurgia Experimental, Faculdade de Medicina de Botucatú, Universidade Estadual Paulista, UNESP.
Spadella
C T
CT
Mercadante
M C
MC
de Macedo
C S
CS
de Macedo
A R
AR
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000601:surgery*; D004386:Duodenum / Q000637:transplantation*; D005260:Female; D007328:Insulin / Q000627:therapeutic use*; D016381:Islets of Langerhans Transplantation; D008297:Male; D016035:Pancreas Transplantation; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D007328:Insulin
Estudo comparativo entre o transplante pancreato-duodenal, transplante de ilhotas de Langerhans e o tratamento com insulina, no controle do diabetes experimental no rato.