Epstein-Barr virus subtype distribution in angioimmunoblastic lymphadenopathy.
The tissues of 16 patients bearing a T-cell lymphoma of angioimmunoblastic lymphadenopathy type (AILD-TCL) were investigated for the distribution of Epstein-Barr virus (EBV) subtypes 1 and 2. EBV-association had been proven in these cases by polymerase chain reaction (PCR) for EBV-DNA, in situ hybridization (ISH) for EBV-encoded small nuclear RNAs (EBER) and immunohistology for EBV-encoded latent membrane protein (LMP). PCR and EBER-ISH produced mostly identical results, but some cases were positive with only one of the 2 methods employed. LMP was detected in a few large cells of 8/13 cases. Twelve cases were investigated for the distribution of EBV subtypes. One case contained EBV genome of subtype 2, 3 cases contained subtype 1 and 4 cases contained both subtypes. Four cases could not be typed. These findings suggest that in AILD, as in AIDS-associated lymphomas and lymphomas of the lethal midline granuloma type, subtype 2 of EBV may occur, perhaps in relation to an immunodysfunction developing progressively in these patients.
Borisch
B
B
Pathologisches Institut, Universität Bern.
Caioni
M
M
Hurwitz
N
N
Dommann-Scherrer
C
C
Odermatt
B
B
Waelti
E
E
Laeng
R H
RH
Kraft
R
R
Laissue
J
J
D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000382:microbiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012342:RNA, Small Nuclear / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014763:Viral Matrix Proteins / Q000032:analysis
D000956:Antigens, Viral; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D012342:RNA, Small Nuclear; D012367:RNA, Viral; D014763:Viral Matrix Proteins