Induction of interleukin-8 expression during cardiopulmonary bypass.
BACKGROUND
Patients undergoing cardiopulmonary bypass (CPB) are known to suffer from a postsurgical systemic inflammatory response, the nature of which remains to be fully elucidated. Interleukin-8 (IL-8) is a newly described, powerful leukocyte chemotactic factor known to be generated after stimulation of interleukin-1 (IL-1). As we have previously documented the generation of IL-1 beta after CPB, it followed that IL-8 generation should be measured in a comparable group of patients.


RESULTS
Twenty-two adult patients aged 41 to 81 years undergoing coronary revascularization were studied for measurements of C3a, C5a, IL-1, IL-8, and OH(.). Blood was collected before surgery, after CPB, and at 24, 48, and 72 hours. A significant increase in IL-1 beta and IL-8 was detected in circulating leukocytes with peak levels at 24 hours after bypass. No IL-1 beta or IL-8 antigen was detected at any time in patient plasma. Comparable to interleukin generation, human complement-derived C5a also peaked after 24 hours, whereas C3a was increased dramatically immediately after CPB, followed by a decline at 24 hours and a progressive increase over the next 48 hours.


CONCLUSIONS
The results demonstrated for the first time the presence of cell-associated IL-8 in CPB patients. This suggests that this powerful polymorphonuclear and T-lymphocyte chemotactic factor may be an important element in leukocyte activation and recruitment after CPB.
Kalfin
R E
RE
Department of Surgery University of Connecticut Health Center, Farmington.
Engelman
R M
RM
Rousou
J A
JA
Flack
J E
JE
Deaton
D W
DW
Kreutzer
D L
DL
Das
D K
DK
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002315:Cardiopulmonary Bypass; D015926:Complement C3a / Q000378:metabolism; D015936:Complement C5a / Q000378:metabolism; D001026:Coronary Artery Bypass; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism; D008875:Middle Aged; D013997:Time Factors
D007375:Interleukin-1; D016209:Interleukin-8; D017665:Hydroxyl Radical; D015926:Complement C3a; D015936:Complement C5a