title: PKC translocation in rat brain cortex is promoted in vivo and in vitro by alpha-glycerylphosphorylcholine, a cognition-enhancing drug.
abstract: Protein kinase C (PKC) activity was measured in soluble and particulate fractions of rat individual brain areas after treatment with alpha-glycerylphosphorylcholine (GPC), a cognition-enhancing drug which promotes acetylcholine synthesis and release. The drug induced both in vivo and in vitro PKC translocation. In vivo, an increase of particulate PKC activity was observed 1 hour following the acute oral administration of a behaviorally active dose (600 mg/kg); the effect was transient. In vitro, GPC promoted PKC translocation in cortical slices at concentrations as low as 50 nM; the concentration-response curve was bell shaped. The increased PKC activity may be related to the cortical effects of GPC.
authors.affiliation: Institute of Pharmacological Sciences, University of Milan, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Govoni
authors.forename: F
authors.initials: F
authors.lastname: Battaini
authors.forename: L
authors.initials: L
authors.lastname: Lucchi
authors.forename: A
authors.initials: A
authors.lastname: Pascale
authors.forename: M
authors.initials: M
authors.lastname: Trabucchi
mesh_terms: D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology*; D003071:Cognition / Q000187:drug effects*; D005997:Glycerylphosphorylcholine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005997:Glycerylphosphorylcholine; D000661:Amphetamine; D011493:Protein Kinase C