Use of Salmonella phage P22 for transduction in Escherichia coli.
A cosmid (pPR1347) carrying both the rfb gene cluster and the rfc gene of a Salmonella group B serovar has been constructed; Escherichia coli K-12 strains carrying this cosmid produce long-chain O antigen, are sensitive to phage P22, and can be transduced by P22. Some of the benefits of P22 transduction are now available for studying E. coli and potentially other genera.
Neal
B L
BL
Department of Microbiology, University of Sydney, New South Wales, Australia.
Brown
P K
PK
Reeves
P R
PR
D017100:Bacteriophage P22 / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005810:Multigene Family; D010957:Plasmids; D011815:R Factors; D015183:Restriction Mapping; D012475:Salmonella / Q000235:genetics*; D014161:Transduction, Genetic