Measurement of glutathione S-transferase pi by radioimmunoassay: elevated plasma levels in lung cancer patients.
Human glutathione S-transferase pi was purified from placenta, and when compared to previous procedures a high yield of pure protein was obtained. A specific and sensitive radioimmunoassay suitable for the measurement of human glutathione S-transferase pi in human plasma is described. The sample collection method for glutathione S-transferase pi was found to be a major obstacle in utilising the measurements for diagnostic use, unless a stringent collection protocol was adhered to. A good discrimination between cancer and non-cancer patients was observed, with 66% of lung cancer patients having elevated glutathione S-transferase pi levels with adenocarcinomas showing the most significant rises.
Howie
A F
AF
Department of Clinical Biochemistry, University of Edinburgh, Royal Infirmary, Scotland, UK.
D000230:Adenocarcinoma / Q000175:diagnosis*; D000328:Adult; D000368:Aged; D001984:Bronchial Neoplasms / Q000175:diagnosis; D018288:Carcinoma, Small Cell / Q000175:diagnosis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D004796:Clinical Enzyme Tests; D005260:Female; D005982:Glutathione Transferase / Q000097:blood*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods; D012680:Sensitivity and Specificity
D005982:Glutathione Transferase