title: Activity gel and activity blotting methods for detecting DNA-modifying (repair) enzymes.
abstract: Zymographical methods (activity gel, overlay gel, activity blot and activity blotting) for detecting DNA-modifying (repair) enzymes are reviewed. Emphasis is put on the novel activity blotting method in which DNA repair enzymes electrophoresed on a gel are blotted and detected on a damaged DNA-fixed nylon membrane. Its practical procedures, including a non-radioactive detection procedure, and representative results are also described.
authors.affiliation: Department of Molecular Biology, Okayama University Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Seki
authors.forename: K
authors.initials: K
authors.lastname: Akiyama
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Tsutsui
mesh_terms: D000818:Animals; D011088:DNA Ligases / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D015151:Immunoblotting / Q000379:methods
chemical_list: D011088:DNA Ligases