Neuronal metabolism of catecholamines: plasma DHPG, DOMA and DOPAC.
Pre-synaptic endings of the sympathetic nervous fibers control the metabolism of catecholamines, particularly inactivating norepinephrine after its neuronal recapture. The present study was carried out to investigate this segment of the metabolism of catecholamines through measurements of DHPG, DOMA and DOPAC concentrations in plasma. A sensitive and specific radio-enzymatic assay was developed of which the major characteristic is to include the plasma sample in the incubation mixture without initial extraction of the deaminated metabolites. In the rat, there was a statistically significant correlation between norepinephrine and DHPG in both anesthetized and conscious conditions and after clonidine or guanethidine induced reduction of sympathetic activity; thus it can be suggested that plasma DHPG is a good index of neuronal metabolism of norepinephrine in this animal. In humans, our data indicate an interesting correlation between norepinephrine and DOMA concentrations in plasma in resting conditions and within three hours after clonidine. Further studies need to be carried out to establish whether DOMA is a better index of neuronal metabolism of norepinephrine than DHPG.
Dong
W X
WX
Department of Medicine, INSERM (U 337), Faculté de Médecine Broussais, Hôtel-Dieu, Paris, France.
Schneider
J
J
Lacolley
P
P
Brisac
A M
AM
Safar
M
M
Cuche
J L
JL
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D003000:Clonidine / Q000494:pharmacology; D004837:Epinephrine / Q000378:metabolism; D006145:Guanethidine / Q000494:pharmacology; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008333:Mandelic Acids / Q000378:metabolism; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D002395:Catecholamines; D008333:Mandelic Acids; D015102:3,4-Dihydroxyphenylacetic Acid; D008734:Methoxyhydroxyphenylglycol; C000989:3,4-dihydroxymandelic acid; D003000:Clonidine; C010117:3,4-dihydroxyphenylglycol; D009638:Norepinephrine; D004837:Epinephrine; D006145:Guanethidine