[The detection and sequence analysis of the simian T-lymphotrophic retrovirus (STLV-1 Papio) by using the polymerase chain reaction].
The gene amplification technique was used for detection and sequence analysis of STLV-1 Papio proviral DNA. The polymerase chain reaction was performed with a primer pair at tax region of HTLV-1, 7336-7354, sense strand, and 7516-7494, antisense strand. One microgram of DNAs isolated from LUG-4 cells and autopsies was used in a reaction volume of 50 microliters involving 30 cycles of amplifications. The reaction product was blunt-end cloned into pUC19 cut with Smal. The sequence was done with T7-polymerase using 32P-dATR as a label. Our results indicate that STLV-1 Papio provirus is actually present in the cells of a lymphoid cell line and tumor cells of lymphomatous monkeys. There are some differences between STLV-1 Papio and reported sequences of HTLV-1 and STLV-1.
D'iachenko
A G
AG
Dzhalagoniia
B E
BE
Kapanadze
B I
BI
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D010215:Papio / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D015303:Simian T-lymphotropic virus 1 / Q000235:genetics* / Q000302:isolation & purification*; D016662:Virus Integration / Q000235:genetics
D004279:DNA, Viral
Vyiavlenie i analiz posledovatel'nosteĭ T-limfotropnogo retrovirusa obez'ian (STLV-1 Papio) pri pomoshchi polimeraznoĭ tsepnoĭ reaktsii.