[Preparation, identification and preliminary application of anti-desmoplakin I monoclonal antibody].
Desmosomes were isolated first from fresh cow nose epidermis. Desmoplakin I (DP I) was purified from desmosome preparations by SDS-polyacrylamide gel electrophoresis and anti-DP I McAb was obtained via hybridoma technique. The tissue specificity showed that all the normal epithelia, different kinds of epithelial tumors and meningioma were positive, whereas non-epithelial tissues and their corresponding tumors gave a negative result. Positive staining showed a series of punctate reaction locating along the cell-cell borders, each spot representing a desmosome or a cluster of closely spaced desmosomes. It is suggested that McAb of DP I is a reliable immunohistochemical probe for epithelial tumor and meningioma diagnosis.
Yang
J L
JL
Department of Pathology, West China University of Medical Sciences, Chengdu.
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D003598:Cytoskeletal Proteins / Q000276:immunology*; D051180:Desmoplakins; D003896:Desmosomes; D003937:Diagnosis, Differential; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D009369:Neoplasms / Q000737:chemistry / Q000473:pathology*
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; C495294:DSP protein, human; D051180:Desmoplakins