Colchicine disposition in human leukocytes after single and multiple oral administration.
Inasmuch as leukocytes were reported to be an active pharmacologic compartment, colchicine disposition was determined in plasma, granulocytes, and mononuclear cells in healthy volunteers after 1 mg oral single and multiple doses. After the single dose, maximal colchicine concentration was observed at 1 hour in plasma and 47 hours later in leukocytes. This delay was confirmed by the slow accumulation of colchicine by lymphocytes in culture. In the multiple-dose study, mean granulocyte colchicine concentration (20 to 53 ng/10(9) cells) were twofold higher than in mononuclear cells (9 to 24 ng/10(9) cells). Mean predicted colchicine multiple-dose granulocyte and mononuclear cell concentrations were 2.5-fold and ninefold higher, respectively, than those measured. After the last dose, colchicine decreased, with half-life values between 41 and 46 hours for leukocytes and 49 hours for plasma. This study validates leukocytes as a microcompartment whose kinetics correlates with colchicine biologic effects.
Chappey
O N
ON
INSERM U26, Hôpital Fernand Widal, Paris, France.
Niel
E
E
Wautier
J L
JL
Hung
P P
PP
Dervichian
M
M
Cattan
D
D
Scherrmann
J M
JM
D000284:Administration, Oral; D000328:Adult; D002478:Cells, Cultured; D003078:Colchicine / Q000008:administration & dosage / Q000097:blood*; D006098:Granulocytes / Q000378:metabolism; D006207:Half-Life; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged
D003078:Colchicine