The hydrophobic and electrostatic effect of basic polyamino acid-DNA polyion complex on artificial bilayer lipid membrane.
Interaction between DNA-polyion complex, such as DNA-poly-L-arginine complex and DNA-histon complex, and artificial bilayer lipid membrane was studied by measuring the transmembrane current fluctuation. DNA did not interact with neutral bilayer lipid membrane. However, with the application of a positive potential, a large current fluctuation was observed in the presence of DNA-poly-L-arginine complex. Using a negative potential, such a current fluctuation was not observed. This current fluctuation was dependent on the polarity of the applied potential. On the other hand, during the application of both positive and negative potential, large current fluctuation was observed in the presence of the DNA-histon complex. These results indicate that DNA-histon complex is able to permeate across the membrane and DNA-poly-L-arginine complex is able to be adsorbed on the hydrophobic part of membrane.
Amao
Y
Y
College of General Education, Faculty of Science, Ibaraki University, Japan.
Kumazawa
N
N
D004247:DNA / Q000737:chemistry*; D006657:Histones / Q000737:chemistry; D008051:Lipid Bilayers; D008564:Membrane Potentials; D010455:Peptides / Q000737:chemistry*; D010539:Permeability
D006657:Histones; D008051:Lipid Bilayers; D010455:Peptides; C015462:polyarginine; D004247:DNA