[Study of the conformational and intradynamic changes of phage lambda DNA molecules by laser correlation spectroscopy].
Conformational changes of lambda phage DNA macromolecules were investigated by dynamic light scattering (DLS) method. It was shown that the persistence length of B-form DNA in some conditions could could decline to approximately 12 nm. When studying the internal motions in the linear form of phage DNA macromolecule, a deviation was observed of the experimental angular dependence of the first cumulant gamma from theoretical predictions for the gamma in Rouse-Zimm model including hydrodynamic interactions. This deviation appeared in the region qRg >> 1, where Rg is gyration radius and q is momentum vector. The first cumulant deviation to higher frequencies is observed only for B-form DNA. Experimental behaviour of the first cumulant gamma for the A-form DNA coincides with the theoretical one in the Rouse-Zimm model.
Arutiunian
A V
AV
Ivanova
M A
MA
Kurliand
D I
DI
Noskin
V A
VA
D010582:Bacteriophage lambda / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry*; D007834:Lasers; D008027:Light; D009690:Nucleic Acid Conformation; D012542:Scattering, Radiation
D004279:DNA, Viral
Izuchenie konformatsionnykh i vnutridinamicheskikh izmeneniĭ molekuly DNK faga lambda metodom lazernoĭ korreliatsionnoĭ spektroskopii.