title: HLA class II DNA typing in two Brazilian populations.
abstract: Brazil constitutes a melting pot of populations arising from three major groups, including Amerindians, Africans, and Europeans predominantly from Portugal who were later supplemented by migrations from other European countries. Although every possible combination of racial mixture exists in Brazil, we have selected for this study two groups of subjects residing in Rio de Janeiro. A predominant White population, among whom some Amerindian admixture may exist, and a predominantly African population having little admixture from the other races. We have used the polymerase chain reaction (PCR) and hybridization with oligonucleotide probes to perform a complete typing of the HLA class II alleles. We report the allele frequencies for HLA-DRB1, DQA1, DQB1 and DPB1. We also report on the postulated DR-DQ haplotypes based on family studies and observations in homozygous B-cell lines. These results may serve as background for various types of clinical studies in Brazilian populations.
authors.affiliation: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas.
authors.forename: M E
authors.initials: ME
authors.lastname: Moraes
authors.forename: M
authors.initials: M
authors.lastname: Fernandez-Viña
authors.forename: I
authors.initials: I
authors.lastname: Salatiel
authors.forename: S
authors.initials: S
authors.lastname: Tsai
authors.forename: J R
authors.initials: JR
authors.lastname: Moraes
authors.forename: P
authors.initials: P
authors.lastname: Stastny
mesh_terms: D000483:Alleles; D044383:Black People / Q000235:genetics*; D001938:Brazil; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006681:HLA-D Antigens / Q000235:genetics*; D006682:HLA-DP Antigens / Q000235:genetics; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D006239:Haplotypes / Q000235:genetics; D000949:Histocompatibility Antigens Class II; D006801:Humans; D008393:Marriage; D016133:Polymerase Chain Reaction; D044469:Racial Groups / Q000235:genetics; D044465:White People / Q000235:genetics*
chemical_list: D006681:HLA-D Antigens; D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II