title: Malignant transformation of cutaneous lesions in renal allograft patients: a role for human papillomavirus.
abstract: Renal allograft recipients are at greatly increased risk of developing squamous cell carcinomas. As these frequently arise adjacent to areas of multiple viral warts, we have investigated a possible role for human papillomavirus in malignant transformation within this population. We established, as primary cultures, keratinocytes from 24 lesions of varying degrees of squamous atypia from 9 patients. Ten of 14 cultures screened for the presence of episomal human papillomavirus DNA were positive using a mixed probe for cutaneous human papillomaviruses, although episomal copy was universally lost with continued passage. Three cultures, 2 of which were derived from malignant tissue and 1 from a benign lesion, were positive when screened with a probe for potentially oncogenic human papillomavirus DNAs 5 or 8. Both positive cultures of malignant origin exhibited extended lifespan and have been briefly characterized by morphology and growth requirements.
authors.affiliation: Department of Experimental Dermatology, Royal London Hospital, England.
authors.forename: K J
authors.initials: KJ
authors.lastname: Purdie
authors.forename: C J
authors.initials: CJ
authors.lastname: Sexton
authors.forename: C M
authors.initials: CM
authors.lastname: Proby
authors.forename: M T
authors.initials: MT
authors.lastname: Glover
authors.forename: A T
authors.initials: AT
authors.lastname: Williams
authors.forename: J N
authors.initials: JN
authors.lastname: Stables
authors.forename: I M
authors.initials: IM
authors.lastname: Leigh
mesh_terms: D001706:Biopsy; D015139:Blotting, Southern; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004279:DNA, Viral / Q000032:analysis; D005500:Follow-Up Studies; D006801:Humans; D007621:Karyotyping; D015603:Keratinocytes / Q000166:cytology / Q000382:microbiology / Q000473:pathology; D016030:Kidney Transplantation / Q000473:pathology*; D027383:Papillomaviridae / Q000302:isolation & purification / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000382:microbiology / Q000473:pathology*; D011183:Postoperative Complications / Q000473:pathology*; D012867:Skin / Q000473:pathology*; D012878:Skin Neoplasms / Q000382:microbiology / Q000473:pathology*; D013997:Time Factors; D014184:Transplantation, Homologous / Q000473:pathology; D014412:Tumor Virus Infections / Q000382:microbiology / Q000473:pathology*
chemical_list: D004279:DNA, Viral