The identification of 5-hydroxy-L-norvaline in cultures of pyridoxine auxotrophs of Escherichia coli B.
Gc-ms and hplc have been employed to identify 5-hydroxy-L-norvaline (L-2-amino-5-hydroxypentanoic acid) in the culture fluids of pyridoxine-starved cultures of two pyridoxine auxotrophs of Escherichia coli B. The production of 5-hydroxynorvaline is shown to be secondary to pyridoxine starvation of the cultures, and the quantities of 5-hydroxynorvaline are similar to those of the common protein amino acids present in these cultures. This is the first time that 5-hydroxynorvaline has been identified as a product of bacterial metabolism.
Hill
R E
RE
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
White
R L
RL
Smith
K C
KC
D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D009154:Mutation; D011736:Pyridoxine / Q000378:metabolism*
D000596:Amino Acids; D003470:Culture Media; C004387:2-amino-5-hydroxyvaleric acid; D011736:Pyridoxine