[Generation and characterization of monoclonal antibody GCF-5 to human giant cell tumor of bone].
Hybrid cell line has been derived from fusion of mouse myeloma (Sp2/0-Ag14) cell and spleen cell from mouse which was immunized with cultured cells of giant cell tumor of bone (GCT). Hybrid cells produce monoclonal antibody against GCT associated antigen in the surface of the tumor cells. The monoclonal antibody was detected by immunoperoxidase staining and was designated by GCF-5. There were more than eighty per cent of tested GCT which showed this antigen. It had no cross reaction with many kinds of normal tissue, HLA antigen, alpha-Fetal Protein, Carcino-Embryonic Antigen and beta-Microglobulin. The subclass of the antibody is IgG1. The hybridoma has been cultured and stored under -196 degrees C for more than eight months and its secretion remains stable.
Zhou
L
L
People's Hospital, Beijing Medical University.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001859:Bone Neoplasms / Q000276:immunology*; D005870:Giant Cell Tumors / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G