[Influences of cyclosporin A on wound healing].
We examine influences of immunosuppression on wound healing, using C57BL/6 mice by carrageenan, anti-asialo GM 1 antibody and Cyclosporin A (CSA). Mice underwent a standardized dorsal skin incision and subcutaneous implantation of sterile polyvinyl alcohol sponge under ether anesthesia. They were sacrificed on the 14th and 21st day after wounding. The pelts excised, and fresh breaking strength was measured with a constant-speed tensiometer. The sponges were removed, and hydroxyproline was measured by Woessner's method. 1) Sixteen mice were divided into 2 groups, eight mice intraperitoneally received carrageenan to suppress macrophage function twice before and after wounding, and eight mice, as a control, received saline in the same way. The breaking strength in the carrageenan-treated mice was weaker than in the control on the 14th day and 21st day. Hydroxyproline content was not different between the treated group and control. 2) Sixteen mice were divided into 2 groups, eight mice intraperitoneally received anti-asialo GM 1 antibody to suppress natural killer cell function twice before and after wounding, and eight mice, as a control, received saline in the same way. The breaking strength in the anti-asialo GM 1 antibody treated mice was not significantly different from that in the control on the 14th or 21st day. Hydroxyproline content was not different in the treated group and control. 3) Thirty two mice were divided into 4 groups, eight mice intraperitoneally received CSA at a dose of 1.0 mg/kg twice before and after wounding, eight mice received CSA at a dose of 10.0 mg/body, eight mice received CSA at a dose of 100.0 mg/body to suppress T helper lymphocyte function, and eight mice, as a control, received Intralipos at a dose of 1.0 g/kg in the same way. The breaking strength in the 10.0 mg/kg and 100.0 mg/kg CSA-treated mice were weaker than in the control on the 14th day. There was no significant difference on the 21st day. The breaking strength in the 1.0 mg/kg treated mice was not different from in the control on the 14th day or 21st day. Histologically, there was no difference between CSA-treated group and control. 4) Forty mice were divided into 4 groups, twenty mice received CSA at a dose of 40.0 mg/kg for once a day for 7 days before and 14 days after wounding respectively, twenty mice received Intralipos at a dose of 1.0 g/kg in the same way.(ABSTRACT TRUNCATED AT 400 WORDS)
Okubo
T
T
Second Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D005677:G(M1) Ganglioside / Q000276:immunology; D006909:Hydroxyproline / Q000032:analysis; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D015291:Transforming Growth Factors / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects* / Q000276:immunology; D014947:Wounds and Injuries / Q000188:drug therapy / Q000473:pathology
D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D015291:Transforming Growth Factors; D016572:Cyclosporine; D002351:Carrageenan; D006909:Hydroxyproline