title: Preeclampsia is not associated with altered platelet vasopressin binding and cytosolic Ca++ concentration.
abstract: OBJECTIVE
  Preeclampsia is an important cause of fetal and maternal morbidity and mortality. Recently it was described that platelet cytosolic Ca++ levels could be used to screen for preeclampsia. The current study investigated platelet arginine vasopressin receptor characteristics, platelet cytosolic Ca++ levels, plasma- and platelet-bound arginine vasopressin in white pregnant women.
  
  
  METHODS
  In a cross-sectional study nine third-trimester nulliparous pregnant women with gestational hypertension (seven with proteinuria, two with excessive weight gain without proteinuria) were compared with nine healthy nulliparous pregnant women matched for gestation length and age and 10 healthy age-matched nonpregnant women. Determined were (1) platelet arginine vasopressin receptor number and affinity, (2) platelet cytosolic Ca++ levels, both basal and on arginine vasopressin or thrombin stimulation, and (3) plasma- and platelet-bound arginine vasopressin levels.
  
  
  RESULTS
  None of the measured parameters differed significantly among the three groups studied. Mean arginine vasopressin receptor number and affinity ranged from 108 to 143 receptors per platelet and 0.35 to 0.40 nmol/L, respectively. A single population of binding sites was found (Hill number 0.96). Basal Ca++ levels ranged from 113.4 to 133.3 nmol/L, on arginine vasopressin stimulation from 199 to 250 nmol/L. Median arginine vasopressin levels in platelet-poor plasma were between 1.2 and 2.4 pg/ml, with circulating platelets being estimated to possess two to five molecules of arginine vasopressin per platelet. A significant correlation was found between platelet cytosolic Ca++ levels before and after arginine vasopressin stimulation (r = 0.69, p < 0.001) and a weak correlation between platelet receptor density and arginine vasopressin-stimulated platelet cytosolic Ca++ levels (r = 0.38, p < 0.05).
  
  
  CONCLUSIONS
  The studied parameters, platelet cytosolic Ca++ levels, whether basal or after stimulation with arginine vasopressin and vasopressin platelet receptor density and affinity, do not discriminate already hypertensive or preeclamptic white women from nondiseased subjects. A valuable test to screen for preeclampsia awaits further prospective studies.
authors.affiliation: Department of Obstetrics and Gynecology, University of Amsterdam, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: van der Post
authors.forename: A
authors.initials: A
authors.lastname: Konijnenberg
authors.forename: K
authors.initials: K
authors.lastname: Boer
authors.forename: M C
authors.initials: MC
authors.lastname: Schaap
authors.forename: C E
authors.initials: CE
authors.lastname: van Boxtel
authors.forename: A
authors.initials: A
authors.lastname: Sturk
authors.forename: G J
authors.initials: GJ
authors.lastname: Boer
authors.forename: D F
authors.initials: DF
authors.lastname: Swaab
mesh_terms: D000328:Adult; D001127:Arginine Vasopressin / Q000097:blood*; D001665:Binding Sites; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D006801:Humans; D009994:Osmolar Concentration; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy
chemical_list: D001127:Arginine Vasopressin; D002118:Calcium