title: Stabilization of egg phosphatidylcholine liposomes by the insertion of sulfatide.
abstract: The characteristic effects of sulfatide and its derivatives on the stability of small unilamellar vesicles of egg phosphatidylcholine liposomes in phosphate-buffered saline were investigated by measuring the leakage of carboxyfluorescein that had been entrapped in these vesicles. We found that both the sulfate group and a long acyl chain, such as lignoceric acid, of the sulfatide are essential for the stabilization. The sulfatide derivatives that contain a somewhat shorter acyl chain such as stearic acid had no effect to suppress the leakage of carboxyfluorescein. The galactose residue of sulfatide is not essential to suppress the leakage. 1H-NMR study using a paramagnetic shift reagent demonstrated that the distribution of phosphatidylcholine in the vesicles containing sulfatide is homogeneous, which seems to contribute to the stability of the membrane.
authors.affiliation: Institute of Applied Biochemistry, Yagi Memorial Park, Gifu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Noda
authors.forename: M
authors.initials: M
authors.lastname: Kurono
authors.forename: N
authors.initials: N
authors.lastname: Ohishi
authors.forename: K
authors.initials: K
authors.lastname: Yagi
mesh_terms: D000818:Animals; D002417:Cattle; D002554:Cerebrosides / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D004355:Drug Stability; D005227:Fatty Acids / Q000032:analysis; D005452:Fluoresceins; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010713:Phosphatidylcholines / Q000737:chemistry*; D010718:Phosphatidylserines / Q000737:chemistry; D013433:Sulfoglycosphingolipids / Q000737:chemistry*
chemical_list: D002554:Cerebrosides; D005227:Fatty Acids; D005452:Fluoresceins; D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D013433:Sulfoglycosphingolipids; C024098:6-carboxyfluorescein; D002784:Cholesterol