A 31P and 1H-NMR investigation in vitro of normal and abnormal human liver.
Spectral changes in human hepatic tumours and possible systemic effects of tumour on host liver were assessed by 31P and 1H in vitro NMR spectroscopy. The 1H and 31P spectra from liver tumour biopsies showed significant elevation in phosphoethanolamine, phosphocholine, taurine, citrate, alanine, lactate and glycine, and significant reduction in GPE (glycerophosphoethanolamine), GPC (glycerophosphocholine), creatine and threonine compared to histologically normal tissue. 31P-NMR spectra obtained from histologically normal tissue within tumour-bearing livers showed significant elevation in phosphoethanolamine and phosphocholine compared to data from liver biopsies from nontumour-bearing patients (pancreatitis). These results suggest that alterations in membrane metabolism in host liver can be detected by 31P-NMR.
Bell
J D
JD
NMR Unit, Hammersmith Hospital, London, UK.
Cox
I J
IJ
Sargentoni
J
J
Peden
C J
CJ
Menon
D K
DK
Foster
C S
CS
Watanapa
P
P
Iles
R A
RA
Urenjak
J
J
D001706:Biopsy; D004983:Ethanolamines / Q000032:analysis; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary; D009682:Magnetic Resonance Spectroscopy; D010195:Pancreatitis / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010767:Phosphorylcholine / Q000032:analysis
D004983:Ethanolamines; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010767:Phosphorylcholine; C002449:glycerophosphoethanolamine; C005448:phosphorylethanolamine