Procollagen types I and III and transforming growth factor-beta gene expression in the arterial wall after exposure to periarterial blood.
The stiffening and thickening of the arterial wall after subarachnoid hemorrhage may reflect increased connective tissue. The purpose of this study was to examine the nature of collagen synthesis in response to periarterial blood. Rat femoral arteries were exposed to periarterial blood for varying lengths of time (control, 1, 3, 7, and 14 d). Dot-blot analysis of total ribonucleic acid extracted from the arteries (n = 10 to 15 animals each) demonstrated that the expression of procollagen Types I and III messenger ribonucleic acid increased at 7 (threefold) and 14 days. The expression of transforming growth factor-beta (TGF-beta), an important regulator of collagen synthesis, was markedly increased by 3 days (threefold), followed by a gradual decline. There were marked differences in procollagen Types I and III and TGF-beta gene expression between arteries exposed to blood and sham-operated arteries for a period of 7 days (n = 25 animals). Northern blot analysis of total ribonucleic acid extracted from cultured vascular smooth muscle cells showed that the treatment with a higher concentration of serum for 48 hours increased the expression of procollagen Types I and III and TGF-beta, whereas exposure to oxyhemoglobin did not. After exposure to periarterial blood, arterial walls show increased synthesis of procollagen Types I and III, perhaps a response to the increased secretion of TGF-beta, which in turn could be the result of exposure to serum factors.
Kasuya
H
H
Department of Surgery, University of Chicago, Illinois.
Weir
B
B
Shen
Y
Y
Hariton
G
G
Vollrath
B
B
Ghahary
A
A
D000818:Animals; D046508:Culture Techniques; D005786:Gene Expression Regulation / Q000502:physiology; D002546:Ischemic Attack, Transient / Q000235:genetics* / Q000473:pathology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D010446:Peptide Fragments / Q000235:genetics*; D011347:Procollagen / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000235:genetics* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000235:genetics; D014655:Vascular Resistance / Q000235:genetics / Q000502:physiology
D010446:Peptide Fragments; D011347:Procollagen; D016212:Transforming Growth Factor beta; C041876:procollagen Type III-N-terminal peptide; C042813:procollagen type I carboxy terminal peptide