Cloning of a gene required for the alkaliphily of alkaliphilic Bacillus sp. strain C-125.
An alkali-sensitive mutant was obtained from alkaliphilic Bacillus sp. strain C-125. A DNA fragment required for the alkaliphily was cloned from the chromosomal DNA of strain C-125 by using the alkali-sensitive mutant as a host. The nucleotide sequence of the cloned DNA fragment indicated the occurrence of several ORFs. Comparison of these ORFs with sequences in the available databases revealed no closely related sequences.
Aono
R
R
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Hayakawa
A
A
Hashimoto
M
M
Kaneko
H
H
Nakamura
S
S
Horikoshi
K
K
D001407:Bacillus / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016366:Open Reading Frames
D004269:DNA, Bacterial