Mono-, di- and triacylglycerol concentrations in human plasma: effects of heparin injection and of a high-fat meal.
A sensitive method has been developed to measure specific mono-, di- and triacylglycerol concentrations in human plasma, using thin-layer chromatography and enzymatic assay. The levels of partial acylglycerols in human plasma from fasting subjects were lower than previous reports had suggested and amounted to less than 3% of the total acylglycerols. After heparin injection the plasma monoacylglycerol concentration increased markedly (P < 0.01) while the triacylglycerol concentration decreased significantly (P < 0.001). The plasma diacylglycerol concentration did not change significantly although it increased as a percentage of the total (P < 0.05); after heparin partial acylglycerols accounted for more than 10% of the total. After a high-fat meal the plasma concentrations of di- and triacylglycerol increased approximately two-fold (P < 0.005) but no significant change was observed for mono-acylglycerol. The percentage contribution of partial acylglycerols was unchanged (2.6% fasting, 2.4% postprandially).
Fielding
B A
BA
Oxford Lipid Metabolism Group, Sheikh Rashid Laboratory, Radcliffe Infirmary, UK.
Humphreys
S M
SM
Allman
R F
RF
Frayn
K N
KN
D000328:Adult; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D004075:Diglycerides / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005990:Glycerol / Q000097:blood*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D008297:Male; D014280:Triglycerides / Q000097:blood
D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004075:Diglycerides; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D006493:Heparin; D005990:Glycerol