title: Relationship between the replication functions of Streptomyces plasmids pJV1 and pIJ101.
abstract: The essential replication region of the Streptomyces phaeochromogenes plasmid pJV1 was sequenced and compared to the equivalent region of the well-characterized Streptomyces plasmid pIJ101. The sequence revealed a similar organization in both plasmids, including a conserved region within both plasmid origins, which is just upstream of a gene encoding the replication protein (Rep). The Rep proteins of pJV1 and pIJ101 are very similar, and both have a conserved motif, also present in Rep proteins of single-stranded DNA plasmids of the low G + C gram+ bacteria that includes a tyrosine residue presumably involved in creating the single-stranded nick that initiates the replication event. A co-integration experiment between these two replicons led to the formation of a novel replicon with a hybrid origin and allowed the identification of the nick site where replication starts, also showing that the replication functions of both plasmids are related.
authors.affiliation: Departamento de Biología Molecular, Universidad Nacional Autónoma de México, Ciudad Universitaria, D.F., México.
authors.forename: L
authors.initials: L
authors.lastname: Servín-González
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D017124:Conserved Sequence; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial