Temporal and spatial expression of serine protease genes in Anopheles gambiae.
Serine proteases play a crucial role during the digestion of the blood meal in the mosquito gut. The isolation and the analysis of the genomic organisation of the corresponding genes may lead to the characterization of gut-specific, inducible promoters, suitable for the expression of anti-parasitic agents in the gut of transgenic mosquitoes. We report here on the identification of a trypsin and a chymotrypsin gene family of Anopheles gambiae. Following a blood meal, the transcription of all members of the two identified gene clusters, seven trypsin genes (Antryp1-7) and two chymotrypsin genes (Anchym1-2), is induced. Recombinant Antryp1 and Antryp2, expressed in E. coli, were both active in vitro against blood proteins. Moreover, mouse sera raised against Antryp1, Anchym1 and Anchym2 recognized the corresponding proteases among the proteins of a lysate prepared from dissected guts of An. gambiae mosquitoes.
Müller
H M
HM
Istituto di Parassitologia, Universita La Sapienza, Rome, Italy.
Vizioli
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I
della Torre
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Crisanti
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A
D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000235:genetics*; D004790:Enzyme Induction; D005247:Feeding Behavior; D005260:Female; D005796:Genes; D007422:Intestines / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D014158:Transcription, Genetic
D012697:Serine Endopeptidases