title: Hormone-induced rise in cytosolic Ca2+ in axolotl hepatocytes: extracellular origin and control by cAMP.
abstract: In amphibian liver, signal transduction of [Arg8]vasotocin (AVT), a "classical" Ca(2+)-dependent hormone in rat liver, is mediated via the generation of adenosine 3',5'-cyclic monophosphate (cAMP) and not via inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]. In isolated hepatocytes from axolotl, hormones that stimulated cAMP formation (the order of efficacy was glucagon > isoprenaline > epinephrine > or = AVT) also provoked a pronounced increase in cytosolic Ca2+, as indicated from changes in fura 2 fluorescence. 8-Bromoadenosine 3',5'-cyclic monophosphate at 100 microM was as potent as maximally effective concentrations of glucagon. Ins(1,4,5)P3 mobilized Ca2+ from the endoplasmic reticulum of saponin-permeabilized axolotl hepatocytes with a half-maximal effect at 0.65 microM, as did GTP (20 microM), even in the absence of polyethylene glycol. However, the hormonally induced increase in cytosolic Ca2+ was not due to a mobilization of the cation from internal stores by Ins(1,4,5)P3, but to an increased inflow from the extracellular medium. We conclude that in axolotl liver, in contrast to rat liver, hormones stimulate the production of cAMP that, in addition to stimulating processes such as glycogenolysis, also regulates the opening of an ion gate in the plasma membrane, which allows the inflow of Ca2+. To our knowledge this is the first demonstration of a second messenger-operated Ca2+ channel in a splanchnic tissue.
authors.affiliation: Division of Biochemistry and Molecular Biology, School of Life Sciences, Australian National University, Canberra.
authors.forename: J W
authors.initials: JW
authors.lastname: Kleineke
authors.forename: P A
authors.initials: PA
authors.lastname: Janssens
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000558:Ambystoma mexicanum; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology*; D005934:Glucagon / Q000494:pharmacology*; D006160:Guanosine Triphosphate / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D013873:Thionucleotides / Q000494:pharmacology; D014668:Vasotocin / Q000494:pharmacology*
chemical_list: D013873:Thionucleotides; D015124:8-Bromo Cyclic Adenosine Monophosphate; C016957:adenosine-3',5'-cyclic phosphorothioate; D015544:Inositol 1,4,5-Trisphosphate; D006160:Guanosine Triphosphate; D005934:Glucagon; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol; D002118:Calcium; D014668:Vasotocin; D004837:Epinephrine; D010646:Phentolamine