[Study on determination of polyactin A by ELISA].
Sandwich ELISA and two-step competition ELISA were utilized to determine polyactin A. After optimization of the experimental conditions, microtiter plates were coated with the rabbit antibody solution and evaporated to dryness at 37 degrees C. The coated microtiter plates were incubated first with the analyte, then with the HRP-labelled antibody (sandwich procedures), or with the HRP-labelled polyactin A (competition procedures), and absorbance was measured after coloration. The whole determination took less than 4 hours, excluding the coating procedure, which is faster than the routine complement fixation method. The coated microtiter plates can be well after over three months of storage at 4 degrees C. The sensitivity obtained was 0.64-7.4 ng/well for the sandwich method or 0.6-1.2 micrograms/well for the competition method.
Yang
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Xu
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D000276:Adjuvants, Immunologic / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006020:Glycopeptides / Q000032:analysis*
D000276:Adjuvants, Immunologic; D006020:Glycopeptides; C064233:polyactin A