title: Epstein-Barr virus genomic sequences and specific antibodies in cerebrospinal fluid in children with neurologic complications of acute and reactivated EBV infections.
abstract: Four children with infectious mononucleosis (IM) and one with reactivated Epstein-Barr virus (EBV) infection had concomitant central nervous system disorders. Cerebrospinal fluid (CSF) samples from all five patients contained EBV genomic sequences and EBV-specific antibodies in the neurologic stage, but not during convalescence. Cerebrospinal fluid from two non-neurologic IM patients had neither EBV DNA nor EBV antibodies. The EBV-positive CSF of the five with neurological disorders were aseptic in culture and all negative for other human herpesvirus DNAs and antibodies: herpes simplex virus types 1 and 2, cytomegalovirus, varicella-zoster virus, and human herpesvirus 6. Epstein-Barr virus DNA and EBV antibodies were not detected in the CSF of 17 EBV-seropositive patients with mumps meningitis, rubella encephalitis, unknown febrile convulsion, or partial epilepsy. It is suggested that EBV plays a causal role in neurologic manifestations in patients with acute and reactivated EBV infections, through direct viral invasion and immunopathological reactions.
authors.affiliation: Department of Virology, Hokkaido University School of Medicine, Sappord, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Imai
authors.forename: N
authors.initials: N
authors.lastname: Usui
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
authors.forename: T
authors.initials: T
authors.lastname: Osato
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Tsutsumi
authors.forename: N
authors.initials: N
authors.lastname: Tachi
authors.forename: S
authors.initials: S
authors.lastname: Nakata
authors.forename: T
authors.initials: T
authors.lastname: Yamanaka
authors.forename: S
authors.initials: S
authors.lastname: Chiba
mesh_terms: D000208:Acute Disease; D000914:Antibodies, Viral / Q000134:cerebrospinal fluid*; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000134:cerebrospinal fluid; D005260:Female; D006566:Herpesviridae Infections / Q000382:microbiology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D007223:Infant; D007244:Infectious Mononucleosis / Q000382:microbiology*; D008297:Male; D008590:Meningoencephalitis / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000382:microbiology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G