Mechanistic aspects of the interaction of antisense DNA.
To investigate the mechanism of antisense effects, diastereoisomerism of oligodeoxynucleotides with modified internucleotide phosphate linkages such as oligodeoxynucleoside phosphoramidates (OPA) and oligodeoxynucleoside phosphorothioates (OPT). Reversed-phase HPLC was used to separate the diastereoisomers of OPA (up to tetramer) and OPT (up to pentamer), and it was found that the complete purification of all diastereoisomers could be achieved by the two-step chromatographic approach. From the circular dichroism measurements, it was found that some diastereoisomers formed certain hybrids with their complementary oligonucleotides and some did not.
Murakami
A
A
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Tamura
Y
Y
Takeguchi
S
S
Miyoshi
H
H
Morii
T
T
Maekawa
T
T
Abe
T
T
Makino
K
K
D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D016373:DNA, Antisense / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism
D016373:DNA, Antisense; D009943:Organophosphorus Compounds