Recognition of non-B DNA structures by cellular proteins.
By utilising a gel retardation assay, cytoplasmic extracts of Hela cells have been shown to contain a number of polypeptides which bind preferentially to non-B forms of DNA. These polypeptides vary in apparent molecular weights and are present as minor components of the total extract. Similar properties were demonstrated by purified preparations of eukaryotic topoisomerase I and by a DNA binding subunit of the transcription factor NFkB. These results suggest that non-B DNA binding proteins may represent families of proteins which recognise more open forms of DNA as a cognate parameter to their cellular functions.
Leith
I R
IR
Division of Cell and Molecular Biology, School of Biological and Medical Sciences, University of St. Andrews, Fife, Scotland, U.K.
Russell
W C
WC
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides; D014157:Transcription Factors; C019530:poly(dC-dG); D004247:DNA; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II