Rod-opsin immunoreaction in the pineal organ of the pigmented mouse does not indicate the presence of a functional photopigment.
The aim of the present study was to characterize the rod-opsin immunoreaction in the mammalian pineal organ. Pigmented mice (strain C57BL) were selected as the animal model. Immunocytochemical investigations involving the use of highly specific polyclonal and monoclonal antibodies against bovine rod-opsin (the apoprotein of the photopigment rhodopsin) showed that approximately 25% of all pinealocytes were rod-opsin immunoreactive. Immunoblotting techniques revealed three protein bands of approximately 40, 75, and 110 kDa; these were detected by the monoclonal antibody and the polyclonal antiserum in retinal and pineal extracts. These protein bands presumably represented the monomeric, dimeric and trimeric forms of rod-opsin. The amount of rod-opsin in retina and pineal organ was quantified by means of an enzyme-linked immunosorbent assay. This yielded 570 +/- 30 pmoles rod-opsin per eye and 0.3 +/- 0.05 pmoles rod-opsin per pineal organ. High pressure liquid chromatography analysis of whole eye extracts demonstrated the chromophoric group of the photopigment rhodopsin, 11-cis retinal, and its isomer, all-trans-retinal. A shift from 11-cis retinal to all-trans-retinal was found upon light adaptation. No retinals were detected in the pineal organ. Autoradiographic investigations showed that 3H-retinol, intraperitoneally injected into the animals, was incorporated into the outer and inner segments of retinal photoreceptors, but not into the pineal organ. It is concluded that the mouse pineal organ contains the authentic apoprotein of rhodopsin but that it lacks retinal derivatives as essential components of all known vertebrate photopigments.(ABSTRACT TRUNCATED AT 250 WORDS)
Kramm
C M
CM
Abteilung Neurobiologie, Johann-Wolfgang-Goethe-Universität, Frankfurt/Main, Germany.
de Grip
W J
WJ
Korf
H W
HW
D000818:Animals; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010786:Photoreceptor Cells / Q000033:anatomy & histology / Q000378:metabolism; D010870:Pineal Gland / Q000033:anatomy & histology / Q000378:metabolism*; D051381:Rats; D012172:Retinaldehyde / Q000378:metabolism; D017299:Rod Opsins / Q000302:isolation & purification / Q000378:metabolism*; D014801:Vitamin A / Q000493:pharmacokinetics
D017299:Rod Opsins; D014801:Vitamin A; D012172:Retinaldehyde