Small subunit rDNA variation in a population of lichen fungi due to optional group-I introns.
A natural population of the lichen-forming ascomycetous fungus, Cladonia chlorophaea, contained individuals with small subunit ribosomal DNA (SSU rDNA) of at least four different size classes and nine restriction-site patterns. The source of these differences was the variable occurrence of 200-400-nucleotide insertions, previously identified as small group-I introns, at five different positions within the SSU coding region. By specific amplification of the sequences flanking these five intron positions with the polymerase chain reaction (PCR), a minimum of nine types of rDNA repeats were defined that differ in number, position, restriction pattern and size of introns. The positions of the introns were verified by sequence analysis. The variable distribution of these introns suggests that they are currently mobile--either by intron insertion, deletion or both--within this species complex.
DePriest
P T
PT
Department of Botany, Duke University, Durham, NC 27708-0342.
D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D006238:Haploidy; D007438:Introns; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D017931:DNA Primers; D004251:DNA Transposable Elements; D004271:DNA, Fungal; D004275:DNA, Ribosomal