title: Ha-ras stimulates the transplasma membrane oxidoreductase activity of C3H10T1/2 cells.
abstract: The ras family of oncogenes, as well as the transplasma membrane electron transport system, have been implicated in cellular growth control. Here we present evidence that the Ha-ras oncoprotein increases the activity of the transplasma membrane electron transport system in the mouse embryonic fibroblast cell line C3H10T1/2. Both cytochrome c and ferricyanide are reduced at a faster rate by C3H10T1/2 cells which are expressing the Ha-ras oncogene. In addition, the Ha-ras transformed cells extrude protons faster in response to stimulation by external oxidants than their normal counterparts. These results suggest that transformation events initiated by the expression of the Ha-ras oncoprotein, p21, in C3H10T1/2 cells have a controlling effect on the transplasma membrane electron transport system.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392.
authors.forename: R A
authors.initials: RA
authors.lastname: Crowe
authors.forename: E J
authors.initials: EJ
authors.lastname: Taparowsky
authors.forename: F L
authors.initials: FL
authors.lastname: Crane
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002471:Cell Transformation, Neoplastic; D003574:Cytochrome c Group / Q000378:metabolism; D004622:Embryo, Mammalian; D011905:Genes, ras; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology; D014162:Transfection
chemical_list: D003574:Cytochrome c Group; D016877:Oxidants; D012402:Rotenone; D000968:Antimycin A; D010088:Oxidoreductases; D016283:Proto-Oncogene Proteins p21(ras)