ARACHnase. An evaluation of a positive control for platelet neutralization procedure testing with seven commercial activated partial thromboplastin time reagents.
ARACHnase (Hemostasis Diagnostics International Co., Denver, CO) is a normal plasma that contains a venom extract from the brown recluse spider, Loxosceles reclusa, which mimics the presence of a lupus anticoagulant (LA). Seven activated partial thromboplastin time (APTT) reagents were used for platelet neutralization procedure (PNP) testing with ARACHnase: Automated APTT (Organon-Teknika, Durham, NC); Thrombofax and Thrombosil (Ortho, Raritan, NJ); Actin and Actin FSL (Dade, Aguado, PR); and Thromboscreen-Kontact and Thromboscreen-APTT LS (Pacific-Hemostasis, Ventura, CA). ARACHnase consistently displayed a positive PNP result of greater than 5 seconds correction of the initial baseline APTT. Thus, ARACHnase may provide a positive control for LA testing, regardless of the choice of APTT reagent and activator/phospholipid combination.
McGlasson
D L
DL
Wilford Hall Medical Center, Lackland AFB, Texas.
Babcock
J L
JL
Berg
L
L
Triplett
D A
DA
D000328:Adult; D005260:Female; D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010314:Partial Thromboplastin Time; D010949:Plasma / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013111:Spider Venoms
D016682:Lupus Coagulation Inhibitor; D010975:Platelet Aggregation Inhibitors; D011933:Reagent Kits, Diagnostic; D013111:Spider Venoms