title: Studies on sperm chromatin structure alterations and cytogenetic damage of mouse sperm following in vitro incubation. Studies on in vitro-incubated mouse sperm.
abstract: Mouse epididymal sperm incubated in Tyrode's T6 fertilization media were analyzed over time for chromosome damage by two methods. First, cytogenetic analysis was done on paternal pronuclei metaphase chromosomes. After 6 hours incubation 11% of the cells demonstrated chromosome structural abnormalities. Secondly, sperm nuclei were measured by the sperm chromatin structure assay, which is a measure of the susceptibility of sperm DNA to the nuclei demonstrated an increased susceptibility to DNA denaturation, reaching near 100% by 48 hours. Changes in chromatin structure at the molecular level may lead to chromosome breaks seen in pronuclear chromosomes.
authors.affiliation: Department of Medical Genetics, West Penn Hospital, Pittsburgh, Pennsylvania 15224.
authors.forename: A M
authors.initials: AM
authors.lastname: Estop
authors.forename: S
authors.initials: S
authors.lastname: Munné
authors.forename: L K
authors.initials: LK
authors.lastname: Jost
authors.forename: D P
authors.initials: DP
authors.lastname: Evenson
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D002843:Chromatin / Q000737:chemistry / Q000648:ultrastructure*; D002875:Chromosomes / Q000737:chemistry / Q000648:ultrastructure; D004247:DNA / Q000032:analysis / Q000648:ultrastructure; D004249:DNA Damage; D005260:Female; D005298:Fertility / Q000502:physiology; D005434:Flow Cytometry; D008297:Male; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011003:Ploidies; D012662:Semen Preservation / Q000379:methods* / Q000592:standards; D013094:Spermatozoa / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure*; D013997:Time Factors
chemical_list: D002843:Chromatin; D004247:DNA