Cloning and sequence of a chicken alpha-N-acetylgalactosaminidase gene.
A cDNA clone encoding the mature polypeptide for the chicken liver alpha-N-acetylgalactosaminidase enzyme was isolated by screening a cDNA library with a synthetic sequence derived from the human alpha-N-acetylgalactosaminidase gene. The deduced amino acid sequence of the chicken liver clone was identical to the amino-terminal and cyanogen bromide derived peptide sequences obtained from the purified enzyme. The chicken enzyme has 75% identity with the human alpha-N-acetylgalactosaminidase and 56-33% identity with alpha-galactosidases from several sources.
Davis
M O
MO
Division of Biological Sciences, University of Missouri-Columbia 65211.
Hata
D J
DJ
Smith
D
D
Walker
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D006596:Hexosaminidases / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D048809:alpha-N-Acetylgalactosaminidase
D018076:DNA, Complementary; D015345:Oligonucleotide Probes; D006596:Hexosaminidases; C482722:NAGA protein, human; D048809:alpha-N-Acetylgalactosaminidase