title: Increase in the ability of human cancer cells to induce cytotoxic T lymphocytes by ultraviolet irradiation.
abstract: The roles of ultraviolet-B (UV) radiation in the immunogenicity of human cancer cells have not been fully studied. We have investigated the effects of UV radiation on metastatic melanoma and renal cell carcinoma cells with regard to MHC antigen expression and the ability to induce cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocytes (TIL) against untreated autologous tumor cells. UV radiation respectively decreased or increased MHC class I expression of freshly isolated tumor cells or cultured tumor cells, and also decreased MHC class I expression of starved cultured tumor cells. It increased the ability of both freshly isolated and cultured tumor cells to induce CTL activity from PBMC against untreated autologous tumor cells. UV-irradiated subclones that were more susceptible to CTL lysis were more potent for CTL induction from TIL than either an untreated parental clone or a UV-irradiated subclone that was resistant to CTL lysis. In summary, UV radiation increased the ability of tumor cells to induce CTL activity without a corresponding effect on MHC antigen expression.
authors.affiliation: Department of Immunology, University of Texas, M.D. Anderson Cancer Center, Houston.
authors.forename: Y
authors.initials: Y
authors.lastname: Umezu
authors.forename: L B
authors.initials: LB
authors.lastname: Augustus
authors.forename: D
authors.initials: D
authors.lastname: Seito
authors.forename: K
authors.initials: K
authors.lastname: Hayakawa
authors.forename: M I
authors.initials: MI
authors.lastname: Ross
authors.forename: O
authors.initials: O
authors.lastname: Eton
authors.forename: D A
authors.initials: DA
authors.lastname: Swanson
authors.forename: K
authors.initials: K
authors.lastname: Itoh
mesh_terms: D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D003602:Cytotoxicity, Immunologic / Q000528:radiation effects; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D008213:Lymphocyte Activation / Q000528:radiation effects; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008545:Melanoma / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000528:radiation effects*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma