Activity of human peritoneal macrophages against a human tumor: role of tumor necrosis factor-alpha, PGE2 and nitrite, in vitro studies.
Human peritoneal macrophages collected from renal patients on continuous ambulatory peritoneal dialysis (CAPD) during inflammation-free periods were induced to express antitumor activity in vitro when cultured in the presence of bacterial lipopolysaccharide (LPS) and even more activity when they were kept in the presence of LPS + IND (indomethacin). The antitumor activity was expressed against a human tumor-cell line, RC43, either in a cell-to-cell contact set-up between the macrophages and the RC43 target cells or when the tumor cells were exposed to supernatants of the cultured macrophages. The antitumor activity of macrophages was correlated to a marked increase in production of tumor necrosis factor-alpha (TNF alpha), not correlated to an increase in nitrite production and inversely correlated to the production of PGE2. The RC43 tumor cells were susceptible to recombinant human TNF alpha, recombinant human IL-1 beta, sodium nitrite and the leukotriene LTB4. The results obtained suggest that activated human macrophages might represent a useful tool for cancer immunotherapy.
Ben-Efraim
S
S
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
Tak
C
C
Fieren
M J
MJ
Romijn
J C
JC
Beckmann
I
I
Bonta
I L
IL
D000818:Animals; D002292:Carcinoma, Renal Cell / Q000276:immunology*; D003470:Culture Media; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D015232:Dinoprostone / Q000096:biosynthesis*; D006801:Humans; D007213:Indomethacin / Q000276:immunology; D007680:Kidney Neoplasms / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008262:Macrophage Activation / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D009573:Nitrites / Q000378:metabolism*; D010531:Peritoneal Dialysis, Continuous Ambulatory; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D003470:Culture Media; D008070:Lipopolysaccharides; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone; D007213:Indomethacin