Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.
The expression at different temperatures of the lacZ gene, which is controlled by the lambda pL and pR tandem promoters and the cI857 temperature-sensitive repressor, was studied in Escherichia coli continuous cultures. At temperatures between 30 and 42 degrees C, beta-galactosidase activity behaved according to an exponential equation. By inducing a culture at a temperature within this range, predefined, nearly constant submaximal levels of gene expression and recombinant product yield can be obtained.
Villaverde
A
A
Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Benito
A
A
Viaplana
E
E
Cubarsi
R
R
D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics; D013696:Temperature; D001616:beta-Galactosidase / Q000235:genetics
D012097:Repressor Proteins; D001616:beta-Galactosidase