title: A link between cyclin A expression and adhesion-dependent cell cycle progression.
abstract: Cell adhesion has an essential role in regulating proliferation during the G1 phase of the cell cycle, and loss of this adhesion requirement is a classic feature of oncogenic transformation. The appearance of cyclin A messenger RNA and protein in late G1 was dependent on cell adhesion in both NRK and NIH 3T3 fibroblasts. In contrast, the expression of Cdc2, Cdk2, cyclin D1, and cyclin E was independent of adhesion in both cell lines. Transfection of NRK cells with a cyclin A complementary DNA resulted in adhesion-independent accumulation of cyclin A protein and cyclin A-associated kinase activity. These transfected cells also entered S phase and complete multiple rounds of cell division in the absence of cell adhesion. Thus, cyclin A is a target of the adhesion-dependent signals that control cell proliferation.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.
authors.forename: T M
authors.initials: TM
authors.lastname: Guadagno
authors.forename: M
authors.initials: M
authors.lastname: Ohtsubo
authors.forename: J M
authors.initials: JM
authors.lastname: Roberts
authors.forename: R K
authors.initials: RK
authors.lastname: Assoian
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016203:CDC2 Protein Kinase / Q000096:biosynthesis; D042846:CDC2-CDC28 Kinases; D002448:Cell Adhesion / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D011494:Protein Kinases / Q000096:biosynthesis; D017346:Protein Serine-Threonine Kinases; D051381:Rats; D014162:Transfection
chemical_list: D016213:Cyclins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D016203:CDC2 Protein Kinase; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; C495893:Cdk2 protein, mouse; C495892:Cdk2 protein, rat; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases