Characterization of myeloid or lymphoid acute leukemia by a chemiluminescence assay. Comparison with immunocytochemistry using an antimyeloperoxidase antibody.
A simple and sensitive chemiluminescence assay for the demonstration of the activity of intracellular myeloperoxidase (MPO) is described, which is useful for the distinction between myeloid and lymphoid commitment in blasts from acute leukemia patients. When the cut-off point was settled at 13 mV of chemiluminescence all cases of acute myeloid leukemia (AML) were distinguished from those of acute lymphoid leukemia. In addition, this technique was able to demonstrate MPO activity in AML poorly differentiated (FAB-M0) which usually does not stain for MPO in classical cytochemistry preparations and could be negative also by immunocytochemistry with anti-MPO monoclonal antibody. Therefore the method here described presented a higher sensitivity than the immunocytochemistry procedure with anti-MPO.
da Fonseca
L M
LM
Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, UNESP, Araraquara, Brazil.
Brunetti
I L
IL
Rego
E M
EM
Garcia
A B
AB
Cilento
G
G
Falcão
R P
RP
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007223:Infant; D007951:Leukemia, Myeloid / Q000175:diagnosis*; D008163:Luminescent Measurements; D008875:Middle Aged; D009195:Peroxidase / Q000276:immunology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D009195:Peroxidase