title: Isolation and mapping of 45 NotI linking clones to chromosome 22.
abstract: Fifty-nine NotI linking clones have been isolated from a flow-sorted chromosome 22 cosmid library and mapped using fluorescence in situ hybridization and/or a panel of somatic cell hybrids. Fourteen clones map to the short arm of chromosome 22, 31 to the long arm, and 9 to other chromosomes; 5 clones could not be unambiguously mapped. To identify potentially informative genetic markers, the chromosome 22 clones were screened for poly(CA) sequences; 24 positively hybridizing clones, 10 on the long arm and 14 on the short arm, were identified. These clones will be useful for constructing a long-range restriction map of chromosome 22 and may facilitate the cloning of chromosome 22 genes.
authors.affiliation: Centre for Research in Neuroscience, McGill University, Montreal, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Sanson
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Demczuk
authors.forename: O
authors.initials: O
authors.lastname: Delattre
authors.forename: P
authors.initials: P
authors.lastname: DeJong
authors.forename: A
authors.initials: A
authors.lastname: Aurias
authors.forename: G
authors.initials: G
authors.lastname: Thomas
authors.forename: G A
authors.initials: GA
authors.lastname: Rouleau
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases