Multiple pre-neoplastic events and clonal selection of radiation induced mouse thymic lymphomas shown by TCR gene rearrangements.
After split-dose irradiation, pre-lymphoma cells develop from a tumor-specific surface antigen TL-2+ thymocyte subpopulation. To analyze the clonality of pre-lymphoma cells, various numbers of TL-2+ thymocytes from a single irradiated mouse were intra-thymically injected to Thyl congenic recipient mice. The incidence of donor type thymic lymphoma(s) was subsequently examined in a group of recipient mice. We chose several lymphomas derived from a single donor mouse and analyzed the TCR gene rearrangements and V(D)J junctional diversity as genetic markers of clonality. These results indicate multiple initial neoplastic events and clonal selection into lymphoma.
Shimizu
T
T
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Muto
M
M
Kubo
E
E
Sado
T
T
Yamagishi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm; D001483:Base Sequence; D015139:Blotting, Southern; D002999:Clone Cells; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D005819:Genetic Markers; D016399:Lymphoma, T-Cell / Q000473:pathology*; D051379:Mice; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced; D011230:Precancerous Conditions / Q000473:pathology*
D000951:Antigens, Neoplasm; D005819:Genetic Markers