A polymerase chain reaction system for rapid diagnosis of scrub typhus within six hours.
A rapid diagnostic system for scrub typhus (tsutsugamushi disease) was established using the polymerase chain reaction (PCR) combined with microplate hybridization. With this system, diagnosis can be made within six hours without the need for radioisotopes, electrophoresis, or membrane hybridization. The use of a PCR processor and DNA-precoated enzyme-linked immunosorbent assay plates allows a rapid and precise diagnosis to be easily made even in primary medical care clinics.
Sugita
Y
Y
Department of Dermatology, Yokohama City University School of Medicine, Japan.
Yamakawa
Y
Y
Takahashi
K
K
Nagatani
T
T
Okuda
K
K
Nakajima
H
H
D000368:Aged; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000097:blood; D001483:Base Sequence; D002850:Chromatography, Gel; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012285:Orientia tsutsugamushi / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D012612:Scrub Typhus / Q000175:diagnosis*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors
D000907:Antibodies, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial