Determination of immune complexes by high-performance gel chromatography (positive cooperativity of antibody-antigen reaction).
Positive cooperativity of antibody-antigen complex formation using human Cu/Zn superoxide dismutase and a murine monoclonal antibody as model is demonstrated by high-performance gel chromatography using TSK G3000sw and TSK G4000sw columns. Three different antibody-antigen complexes named Type I, II and III composed from two antigen molecules+two antibody molecules, two antigen molecules+one antibody molecule and one antigen molecule+one antibody molecule could be separated. The degree and ratio of complexes present in solution depends mainly on the ratios of antibody and antigen.
Schroll
J
J
Institute of Applied Microbiology, School of Food and Biotechnology, University of Bodenkultur, Vienna, Austria.
Necina
R
R
Tauer
C
C
Vorauer
K
K
Plot
R
R
Jungbauer
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007700:Kinetics; D051379:Mice; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D013482:Superoxide Dismutase