Novel, plasmid-encoded, TEM-derived extended-spectrum beta-lactamase in Klebsiella pneumoniae conferring higher resistance to aztreonam than to extended-spectrum cephalosporins.
A clinical isolate of Klebsiella pneumoniae was more resistant to aztreonam than to cefotaxime and ceftazidime. It produced a clavulanate-susceptible beta-lactamase with an isoelectric point of 6.3 which readily hydrolyzed penicillins, cefotaxime, and ceftazidime, but which hydrolyzed aztreonam poorly. The enzyme was encoded by a gene on a 15-kb plasmid; the gene hybridized with an intragenic DNA probe of blaTEM.
Arlet
G
G
Service de Microbiologie, Hôpital Saint-Louis, Paris, France.
Rouveau
M
M
Fournier
G
G
Lagrange
P H
PH
Philippon
A
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001398:Aztreonam / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000096:biosynthesis; D006801:Humans; D007525:Isoelectric Focusing; D007700:Kinetics; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D010641:Phenotype; D010957:Plasmids; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D015342:DNA Probes; D004269:DNA, Bacterial; D065093:beta-Lactamase Inhibitors; C055184:beta-lactamase TEM-3; D001618:beta-Lactamases; D001398:Aztreonam