Effects of adrenergic stimulants on the splenic diameter, haemoglobin content and haematocrit in anaesthetized dogs: determination of the adrenoceptor subtype responsible for changes in the splenic diameter.
Changes in splenic diameter measured by sonomicrometry in response to various adrenergic stimulants were estimated together with simultaneously measured arterial haemoglobin content (HGB) and haematocrit (HCT) in anaesthetized dogs. Splenic diameter decreased following intravenous injections (i.v.) of adrenaline, noradrenaline and phenylephrine and splenic nerve stimulation associated with increases in arterial HGB and HCT, which were significantly attenuated by prazosin i.v. After prazosin i.v., adrenaline i.v. increased splenic diameter significantly, but noradrenaline i.v. did not. Isoprenaline i.v. increased splenic diameter transiently, followed by a decrease that was abolished by prazosin i.v. During occlusion of splenic arteries and veins, adrenaline i.v. and phenylephrine i.v. did not cause any change in arterial HGB and HCT. Injection to splenic artery (i.a.) of phenylephrine induced a significant decrease in splenic diameter that was attenuated by prazosin i.a. but not by yohimbine i.a. Clonidine i.a. did not change splenic diameter. The present results indicate that splenic contraction, which is mediated through alpha 1-adrenoceptor activation, causes a significant increase in arterial HGB and HCT.
Ojiri
Y
Y
Department of Pharmacology, University of the Ryukyus, Japan.
Noguchi
K
K
Chibana
T
T
Sakanashi
M
M
D000758:Anesthesia; D000818:Animals; D004285:Dogs; D004558:Electric Stimulation; D005260:Female; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism*; D008297:Male; D011941:Receptors, Adrenergic / Q000187:drug effects*; D018340:Receptors, Adrenergic, alpha-1 / Q000187:drug effects; D018341:Receptors, Adrenergic, alpha-2 / Q000187:drug effects; D018343:Receptors, Adrenergic, beta-2 / Q000187:drug effects; D013154:Spleen / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D013157:Splenic Artery / Q000187:drug effects / Q000502:physiology; D013162:Splenic Vein / Q000187:drug effects / Q000502:physiology; D013566:Sympathomimetics / Q000494:pharmacology*
D006454:Hemoglobins; D011941:Receptors, Adrenergic; D018340:Receptors, Adrenergic, alpha-1; D018341:Receptors, Adrenergic, alpha-2; D018343:Receptors, Adrenergic, beta-2; D013566:Sympathomimetics