Steroid hormones regulate cAMP and cGMP production by porcine granulosa cells in vitro.
The influence of steroid hormones on the output of the cyclic nucleotides adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) by porcine granulosa cells was investigated. Both progesterone (100, 1000, 10,000 and 100,000 pg/ml medium) and estradiol (100, 1000, 10,000 and 100,000 pg/ml medium) activated cAMP and cGMP production. Testosterone (100 or 1000 pg/ml medium) also stimulated cAMP output. The stimulating effect of steroid hormones on cyclic nucleotide production may suggest the involvement of cAMP- and cGMP-dependent intracellular mechanisms in the action of steroid hormones on porcine ovarian cells.
Sirotkin
A V
AV
Research Institute of Animal Production, Nitra, Slovakia.
Nitray
J
J
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D004958:Estradiol / Q000502:physiology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000502:physiology*; D006107:Granulosa Cells / Q000378:metabolism*; D011374:Progesterone / Q000502:physiology; D013552:Swine; D013739:Testosterone / Q000502:physiology
D012739:Gonadal Steroid Hormones; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D000242:Cyclic AMP; D006152:Cyclic GMP