Transcription of mononucleosomal particles acetylated in the presence of n-butyrate.
Although a correlation between chemical acetylation of the amino-terminal tails of core histones and stimulation of RNA synthesis has been reported for nucleosomal core particles (Piñeiro et al. (1991) Biochem. Biophys. Res. Commun. 177:370), no differences in transcription are detected between acetylated and nonacetylated mononucleosomal particles obtained from HeLa cells in the presence and absence of n-butyrate. Apparently, the lysine residues modified in the presence of n-butyrate are not the same responsible for the observed acetylation-induced transcription. The acetylation obtained with n-butyrate might be significantly different from that present in transcriptionally active chromatin.
Piñeiro
M
M
Centro de Biología Molecular, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Hernández
F
F
Puerta
C
C
Palacián
E
E
D000107:Acetylation; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D009707:Nucleosomes / Q000378:metabolism*; D014158:Transcription, Genetic
D002087:Butyrates; D009707:Nucleosomes; D020148:Butyric Acid