title: Evaluation of bone-wax extract on the frequency of chromosomal aberrations in the bone marrow of Swiss mice.
abstract: Indigenously developed bone wax was evaluated for cytogenetic effects of a saline extract of the bone wax. Swiss mice were assigned to 5 groups of 6 animals each. Groups I, II, III were given non-lethal doses of 2.5, 5.0 or 10.0 ml bone-wax saline extract/kg body wt and Groups IV and V received sterile 0.9% saline vehicle or cyclophosphamide ip as controls. After 24 h the mice were sacrificed by cervical dislocation. Bone marrow preparations were stained with Giemsa's stain and examined by light microscopy. Abnormalities such as numbers of chromosome gaps, breaks and translocations were noted. The bone-wax saline extract did not induce chromosomal aberrations in the bone marrow of Swiss mice under our laboratory conditions.
authors.affiliation: Division of Toxicological Screening of Materials, Sree Chitra Thirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
authors.forename: P V
authors.initials: PV
authors.lastname: Mohanan
authors.forename: K
authors.initials: K
authors.lastname: Rathinam
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced; D025063:Chromosome Disorders; D002875:Chromosomes / Q000187:drug effects*; D003520:Cyclophosphamide / Q000494:pharmacology; D004247:DNA / Q000187:drug effects; D004338:Drug Combinations; D005260:Female; D006490:Hemostatics / Q000633:toxicity*; D008297:Male; D051379:Mice; D010168:Palmitates / Q000633:toxicity*; D014885:Waxes / Q000633:toxicity*
chemical_list: D004338:Drug Combinations; D006490:Hemostatics; D010168:Palmitates; D014885:Waxes; C055480:bone wax; D003520:Cyclophosphamide; D004247:DNA