Differential effect of chronic ethanol treatment on barbiturate and steroid modulation of muscimol-binding to rat brain cortex.
We report the differential alterations produced by chronic ethanol treatment on the modulation, by the barbiturate thiopental and the steroid 5 beta-pregnan-3 alpha-ol-20-one, of the binding of [3H]muscimol to membrane preparations from rat brain cortex. We found a clear barbiturate- and steroid-promoted enhancement of muscimol-binding to membranes in both control and ethanol-treated animals. However, the enhancements were higher in control animals, using the barbiturate, and in ethanol-treated rats, using the steroid, Bmax and Kd values were also differentially affected in control and ethanol-treated animals by the presence of the barbiturate or the steroid.
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Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
Casanova
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Chinchetru
M A
MA
Fernández-López
A
A
Calvo
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P
D000818:Animals; D001463:Barbiturates / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009118:Muscimol / Q000378:metabolism*; D011280:Pregnanolone / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology*; D013874:Thiopental / Q000494:pharmacology
D001463:Barbiturates; D011963:Receptors, GABA-A; D013256:Steroids; D009118:Muscimol; D000431:Ethanol; D011280:Pregnanolone; D013874:Thiopental