title: Cloning and sequencing of two new Verotoxin 2 variant genes of Escherichia coli isolated from cases of human and bovine diarrhea.
abstract: We cloned and sequenced two new Verotoxin 2 (VT2) variant genes: one from an Escherichia coli strain from a case of bovine diarrhea and the other from an E. coli strain from a patient with diarrhea. The nucleotide and amino acid sequences of these two genes were highly homologous with, but distinct from those of the VT2, VT2vha, VT2vhb, SLT-IIv (VT2vp1) and SLT-IIva (VT2vp2) genes. Their nucleotide sequences were much more closely homologous to that of VT2vh than to that of VT2vp. Search for these two new genes in other Verocytotoxin-producing E. coli strains resulted in the isolation of 2 strains carrying one of the new VT2 variant genes, one strain from Tokyo and the other from Canada.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: Z
authors.initials: Z
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Yamasaki
authors.forename: H
authors.initials: H
authors.lastname: Kurazono
authors.forename: M
authors.initials: M
authors.lastname: Ohmura
authors.forename: T
authors.initials: T
authors.lastname: Karasawa
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: S
authors.initials: S
authors.lastname: Sakamoto
authors.forename: T
authors.initials: T
authors.lastname: Suganami
authors.forename: T
authors.initials: T
authors.lastname: Takeoka
authors.forename: Y
authors.initials: Y
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology* / Q000662:veterinary; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017385:Sequence Homology; D022641:Shiga Toxin 2; D014709:Vero Cells
chemical_list: D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins; D022641:Shiga Toxin 2