title: Interferon-alpha and interferon-gamma induced modulation of proteins in human corneal fibroblasts.
abstract: Little is known about the effects of interferon (IFN) on cell function in the eye. We have analyzed the effect of INF-alpha and IFN-gamma on the expression of proteins in cultured human corneal fibroblasts. Treatment with IFN-alpha increased the synthesis of proteins of 84, 76, 52, and 28 kD and decreased the synthesis of a 72-kD protein. Treatment with IFN-gamma increased the synthesis of proteins of 83, 66, 64, 54, and 47 kD. The effect of IFN-alpha and IFN-gamma were first detected at 5-9 h and 9 h, respectively, after the addition of the IFNs and were maximal at 17 and 24 h, respectively. Most of the changes were seen at doses of 1 x 10(1) to 1 x 10(2) U/ml of IFN-alpha or IFN-gamma and were maximal at 1 x 10(2) to 1 x 10(3) U/ml. Thus, each IFN induced distinct proteins based on apparent molecular weight and isoelectric point. These results show that IFN-alpha and IFN-gamma affect the synthesis of small groups of distinct proteins in human corneal fibroblasts.
authors.affiliation: Department of Ophthalmology, University of Wisconsin, Madison 53706-1532.
authors.forename: M J
authors.initials: MJ
authors.lastname: Balish
authors.forename: M E
authors.initials: ME
authors.lastname: Abrams
authors.forename: J W
authors.initials: JW
authors.lastname: Chandler
authors.forename: C R
authors.initials: CR
authors.lastname: Brandt
mesh_terms: D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005136:Eye Proteins / Q000096:biosynthesis / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008970:Molecular Weight
chemical_list: D005136:Eye Proteins; D016898:Interferon-alpha; D007371:Interferon-gamma