title: [Structural and immunologic studies of Proteus mirabilis 033 O-specific polysaccharide].
abstract: O-specific polysaccharide was obtained by mild acid degradation of P. mirabilis 033 lipopolysaccharide (LPS). It was found to contain N-acetyl-D-glucosamine, D-glucuronic acid and N-acetyl-L-fucosamine in a ratio 1:1:1. On the basis of the data obtained from 13C-NMR and methylation analysis, the following structure of repeating unit was established: [formula: see text] Selective removal of the D-GlcA significantly decreased reactivity of 033 O-specific polysaccharide with homologous antiserum. This component was plays an immunodominant role. Cross reactivity between anti-033 serum and disaccharide alfa-L-FucNAc-beta-D-GlcNAc containing P. vulgaris 023 and S. arizonae 059 O-specific polysaccharides was also observed.
authors.affiliation: Instytut Mikrobiologii i Immunologii Uniwersytetu Lódzkiego.
authors.forename: M
authors.initials: M
authors.lastname: Cedzyński
authors.forename: A
authors.initials: A
authors.lastname: Rózalski
authors.forename: K
authors.initials: K
authors.lastname: Kotełko
authors.forename: W
authors.initials: W
authors.lastname: Kaca
authors.forename: E V
authors.initials: EV
authors.lastname: Vinogradov
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: N K
authors.initials: NK
authors.lastname: Kochetkov
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis; D003429:Cross Reactions; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011134:Polysaccharides / Q000032:analysis*; D011513:Proteus mirabilis / Q000737:chemistry* / Q000145:classification; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D011134:Polysaccharides
vernacular_title: Badania strukturalne i immunologiczne polisacharydu O-swoistego Proteus mirabilis 033.