The use of Daphnia magna for detection of okadaic acid in mussel extracts.
Okadaic acid (OA), the main toxin responsible for diarrheic shellfish poisoning (DSP) has toxicity for Daphnia magna (EC50 = 15 +/- 1.8 micrograms/l). A Daphnia bioassay was developed and used to analyse okadaic acid in mussel extracts. A linear correlation was found between OA concentration determined by the Daphnia bioassay and by HPLC assay (r = 0.85; p < 0.001). The Daphnia bioassay can measure OA levels 10 times below the threshold of the mouse bioassay method. It is an inexpensive sensitive tool which can be used in replacement of mouse bioassay for the screening of OA and some co-extracting toxins in mussel extracts.
Vernoux
J P
JP
Laboratoire de Physiologie, Cellulaire et Moléculaire-Université de Caen Esplanade de la Paix, France.
Le Baut
C
C
Masselin
P
P
Marais
C
C
Baron
B
B
Choumiloff
R
R
Proniewski
F
F
Nizard
G
G
Bohec
M
M
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003621:Daphnia; D004988:Ethers, Cyclic / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007476:Ionophores / Q000032:analysis; D019319:Okadaic Acid; D012758:Shellfish / Q000032:analysis*
D004988:Ethers, Cyclic; D007476:Ionophores; D019319:Okadaic Acid