title: Compartmentation of the cerebellar cortex by protein kinase C delta.
abstract: Six isozymes of protein kinase C were analysed in the rat cerebellum using immunohistochemistry. The results revealed a non-uniform distribution of protein kinase C delta among Purkinje, basket, and stellate cells. Serial-section mapping of the delta immunoreactivity revealed that (i) the number and intensity of labeled Purkinje cells increased from rostral to caudal while labeled basket-stellate cells decreased caudally; (ii) the majority of Purkinje cells were labeled in the nodulus, flocculus, and paraflocculus while the anterior lobules were mostly negative; and (iii) labeled Purkinje cells formed distinct parasagittal bands in lobules 6-9 of the paravermis and vermis. The banding of protein kinase C delta within subsets of Purkinje cells suggests units of cerebellar circuitry with specific signaling properties through protein phosphorylation. The visual-vestibular regions of the cerebellum contained the highest amount of the isozyme.
authors.affiliation: Department of Otolaryngology, New York University Medical Center, NY 10016.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: D E
authors.initials: DE
authors.lastname: Hillman
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000166:cytology / Q000201:enzymology*; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis*; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D011493:Protein Kinase C / Q000032:analysis*; D011689:Purkinje Cells / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C