Acetylcholine receptor subunit gene expression in thymic tissue.
It is controversial whether acetylcholine receptors (AChR) or AChR-like proteins exist in human thymus. To evaluate this question we isolated RNA from paraffin-embedded thymic tissue of 5 myasthenics and 5 nonmyasthenics. RNA was subjected to reverse transcription followed by polymerase chain reaction (RT-PCR) using primers specific for beta-actin, subunits of the fetal- and adult-type AChR, and Myf-4, a gene product which regulates AChR expression in muscle. beta-Actin transcripts were identified in specimens from 8 of 10 patients. In these 8 patients alpha- and epsilon-subunit transcripts were identified. Presence of these transcripts did not correlate with thymic pathology or clinical presentation. No gamma-subunit (specific for the fetal-type AChR) or Myf-4 transcripts were found. Our results indicate that mRNA for subunits of the adult-type AChR are expressed in thymic tissue by mechanisms not involving Myf-4. AChR subunits in pathological thymus may provide a target for immune attack in MG. However, as AChR subunits were found in thymus tissue from myasthenics and nonmyasthenics, the presence of AChR subunits in thymus alone is not sufficient to produce myasthenia gravis.
Kaminski
H J
HJ
Department of Neurology 127W, Cleveland Veterans Affairs Medical Center, OH 44106.
Fenstermaker
R A
RA
Abdul-Karim
F W
FW
Clayman
J
J
Ruff
R L
RL
D000199:Actins / Q000096:biosynthesis; D000328:Adult; D001483:Base Sequence; D017931:DNA Primers; D005333:Fetus; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009157:Myasthenia Gravis / Q000378:metabolism / Q000601:surgery; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D011950:Receptors, Cholinergic / Q000096:biosynthesis*; D013934:Thymectomy; D013950:Thymus Gland / Q000378:metabolism*; D014158:Transcription, Genetic
D000199:Actins; D017931:DNA Primers; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011950:Receptors, Cholinergic