title: A C-terminal fragment of the hormone stanniocalcin is bioactive in eels.
abstract: An in vivo eel bioassay based on 45Ca uptake from the ambient medium was used to compare the activity of native trout and American eel stanniocalcin (STC) with that of a synthetic C-terminal fragment (AA 202-231; peptide W) of Australian eel STC. Qualitatively, the action of peptide W resembled that of purified native rainbow trout (Oncorhynchus mykiss) STC as well as fresh extracts of American eel Stannius corpuscles in that they all inhibited whole animal Ca2+ influx. This inhibition was observed in both intact and stanniectomized eels regardless of whether the eels were exhibiting high or low initial calcium uptake rates. These observations suggest that peptide W activates eel gill STC receptors. Further, as peptide W was previously reported to stimulate Ca2+ influx in intact rainbow trout fry, the present data provides evidence for a difference in the way this fragment functions in various species.
authors.affiliation: Department of Biology, University of Ottawa, Ontario, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Fenwick
authors.forename: P
authors.initials: P
authors.lastname: Verbost
mesh_terms: D000811:Anguilla / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002846:Chromatography, Affinity; D005880:Gills / Q000502:physiology; D006023:Glycoproteins / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D006728:Hormones / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D017686:Oncorhynchus mykiss / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology*
chemical_list: D002132:Calcium Radioisotopes; D006023:Glycoproteins; D006728:Hormones; D010446:Peptide Fragments; C051795:teleocalcin; D002118:Calcium