[Purification and anticancer activity of cytotoxin-14 from venom of Naja naja atra].
Ion-chromatography of lyophilized cobra (Naja naja atra) venom on SP-Sephadex C-50 yielded 14 fractions, of which 7, 8, 9, and 10 possessed neurotoxic actions and 11, 12, 13, and 14 had cytotoxic activities. Chromatography of fraction 14 on SP-Sephadex C-25 gave cytotoxin-14. It was homogeneous on polyacrylamide gel electrophoresis with m = 7448 Da. It was a typical cytotoxin without phospholipase A2 activity and had a selective cytotoxic action on human cancer cell lines.
Zhong
X Y
XY
Laboratory of Venom Research, Fujian Medical College, Fuzhou, China.
Liu
G F
GF
Wang
Q C
QC
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D003603:Cytotoxins / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D004546:Elapid Venoms / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D007928:Lethal Dose 50; D009200:Myocardial Contraction / Q000187:drug effects; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; C081128:CT14 cytotoxin, Naja naja atra; D003603:Cytotoxins; D004546:Elapid Venoms