Influence of bacteria on electrophoretic proteinase patterns of Entamoeba histolytica isolates.
Gelatin SDS- polyacrylamide gel electrophoresis was used to compare proteinase banding patterns under reducing conditions from whole cell lysates of four axenic and four xenic pathogenic strains of Entamoeba histolytica. All strains shared major bands in the 34 and 66-68 kDa regions, whereas only the axenic strains produced major bands at 26, 28, 30 and 45 kDa. One axenic strain, NIH 200, when reassociated with mixed bacterial flora, reverted to an electrophoretic banding pattern characteristic of other xenic strains. These results suggest that the 26-30 kDa and 45 kDa proteinases of E. histolytica are induced by bacterial starvation while others are constitutively expressed. It is also proposed that the axenic bands of 45 and 34 kDa represent respectively, the reduced forms of the 56 and 40 kDa bands reported elsewhere under non-reducing conditions.
Spice
W M
WM
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, U.K.
Ackers
J P
JP
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000254:growth & development; D005780:Gelatin / Q000378:metabolism; D015800:Protozoan Proteins / Q000302:isolation & purification*
D015800:Protozoan Proteins; D005780:Gelatin; D010450:Endopeptidases