Regulation of spermidine transport in L1210 cells.
1. 1 mM 2-amino isobutyric acid (AIB), glutamine or asparagine when preincubated for 3 hr with L1210 cells promoted a marked increase in the rate of spermidine uptake. 2. Cycloheximide also increased the transport rate and completely prevented the increase due to AIB. 3. Trifluoperazine and iso-H7 inhibited the uptake of spermidine, much less the uptake of AIB. 4. Adenosine promoted an increase in the uptake of AIB, a decrease in that of spermidine. 5. Hypotonic stress also increased the rate of spermidine transport. This modification was only partially prevented by cycloheximide. 6. Okadaic acid had no effect on this increase, whereas it prevented the increase of ODC activity.
Fontana
L
L
Dipartimento di Medicina e Oncologia Sperimentale, Universita' di Torino, Turin, Italy.
Colombatto
S
S
Grillo
M A
MA
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D007939:Leukemia L1210 / Q000378:metabolism*; D051379:Mice; D013095:Spermidine / Q000493:pharmacokinetics*
D013095:Spermidine