Lysosomal enzyme activities in liver and sera from guinea pigs fed oil related to the toxic oil syndrome.
beta-N-Acetylglucosaminidase and beta-galactosidase activities were determined in serum and liver from guinea pigs fed "toxic oil" (related to cases of TOS) under different experimental conditions. The results obtained were compared with those of guinea pigs fed non "toxic oil" (case-unrelated oil; controls 1) and animals fed no oil (controls 2). In serum, both activities were significantly increased after all treatments with case-related oil as compared with controls 1 and 2. In the liver, beta-galactosidase activity did not show significant differences in any of the treatments when compared with controls 2. However, NAG activity decreased significantly after 7 days of treatment with non-heated oil--either case-related or not--when compared with controls 2; it also decreased significantly after 28 days of treatment with heated case-unrelated oil, both with respect to controls 2 and the animals fed case-related oil. Liver weights tended to increase in the animals fed oil--toxic or not--with respect to those of the livers from untreated animals. Morphologically, a slight vacuolization of the hepatocytes was observed in some of the samples from the treated animals.
Sánchez-Martín
M M
MM
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Spain.
Sánchez-Bernal
C
C
Sánchez-Llorente
A
A
Cabezas
J A
JA
Pérez-González
N
N
D000118:Acetylglucosaminidase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D001937:Brassica; D005229:Fatty Acids, Monounsaturated; D005517:Foodborne Diseases / Q000209:etiology; D006168:Guinea Pigs; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology*; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010938:Plant Oils / Q000506:poisoning*; D011506:Proteins / Q000378:metabolism; D000074262:Rapeseed Oil; D013577:Syndrome; D001616:beta-Galactosidase / Q000097:blood / Q000378:metabolism*
D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D011506:Proteins; D000074262:Rapeseed Oil; D001616:beta-Galactosidase; D000118:Acetylglucosaminidase