A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide).
Poly(N-isopropyl acrylamide) (PIPAAm) demonstrated a fully expanded chain conformation below 32 degrees C and a collapsed, compact conformation at high temperatures. This unique temperature responsive polymer was grafted onto surfaces of commercial polystyrene dishes and used as temperature switches for creating hydrophilic surfaces below 32 degrees C and hydrophobic surfaces above 32 degrees C. Cell attachment and the growth of bovine endothelial cells and rat hepatocytes on PIPAAm-grafted surfaces at 37 degrees C demonstrated similar behavior to the commercialized culture dishes. Both cell types were observed to detach from the PIPAAm-grafted surface simply by reducing the temperature below the polymer transition temperature (collapse). Cells recovered by this method maintained substrate adhesivity, growth, and secretion activities nearly identical to those found in primary cultured cells in contrast to the compromised function found in cultured cells damaged by trypsinization. These results provide strong evidence that PIPAAm-grafted surfaces, as thermal switches are very effective for reversing cell attachment and detachment without cell damage. Properties of cell culture surfaces can be readily transformed by this technique reversibly into hydrophilic and hydrophobic coatings of PIPAAm-grafted polymers.
Okano
T
T
Institute of Biomedical Engineering, Tokyo Women's Medical College, Japan.
Yamada
N
N
Sakai
H
H
Sakurai
Y
Y
D000180:Acrylic Resins; D000818:Animals; D001013:Aorta, Thoracic; D001672:Biocompatible Materials; D001769:Blood; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis; D004730:Endothelium, Vascular / Q000166:cytology* / Q000648:ultrastructure; D007700:Kinetics; D008099:Liver / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D011137:Polystyrenes; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000096:biosynthesis; D013997:Time Factors
D000180:Acrylic Resins; D001672:Biocompatible Materials; D011137:Polystyrenes; D012709:Serum Albumin; C052970:poly-N-isopropylacrylamide; D004247:DNA