NF kappa B upstream regulatory sequences of the HIV-1 LTR are involved in the inhibition of HIV-1 promoter activity by the NS proteins of autonomous parvoviruses H-1 and MVMp.
To investigate parvoviral interference with human immunodeficiency virus type 1 (HIV-1) in human cells that are normally susceptible to HIV-1 infection, nonstructural (NS) proteins of the parvoviruses H-1 virus and minute virus of mice were studied for their effect on the activity of the HIV-1 promoter in a variety of CD4+ cells. Transient cotransfection assays revealed a reduced HIV-1 promoter activity in the presence of parvoviral NS proteins. Stimulation of the HIV-1 promoter by phorbol 12-myristate 13-acetate (PMA) led to an increase in its sensitivity to NS-induced suppression. The inhibitory effect of NS polypeptides depended, at least in part, on the presence of the NF kappa B motifs of the HIV-1 long terminal repeat, suggesting an interaction of the parvoviral products with PMA-inducible cellular factors binding to these elements of the HIV-1 promoter.
Faisst
S R
SR
Unité d'Oncologie Moléculaire, CNRS 1160, Institut Pasteur de Lille, France.
Faisst
S
S
Grangette
C
C
Schlehofer
J R
JR
Rommelaere
J
J
D000818:Animals; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D017930:Genes, Reporter; D016325:HIV Long Terminal Repeat / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008915:Minute Virus of Mice / Q000235:genetics / Q000254:growth & development; D016328:NF-kappa B / Q000378:metabolism; D017992:Parvovirus / Q000235:genetics / Q000254:growth & development*; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D013489:Suppression, Genetic; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014762:Viral Interference / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000494:pharmacology*
D016328:NF-kappa B; D017361:Viral Nonstructural Proteins; D008156:Luciferases; D013755:Tetradecanoylphorbol Acetate