title: Effect of furosemide and weight carriage on energetic responses of horses to incremental exertion.
abstract: The effect of furosemide-induced weight loss on the energetic responses of horses to running was examined in a 3-way crossover study. Eight 2- to 3-year-old Standardbred mares received, in random order, 10 ml of saline solution 4 hours before running on a treadmill (control trial, C); or, during 2 trials, 1 mg of furosemide/kg of body weight, i.v., 4 hours before running. During one of the trials when the horses received furosemide, they carried weight equal to that lost over the 3.75 hours after furosemide administration while running (furosemide-loaded, FL), and during the other trial they did not carry weight equal to that lost after furosemide administration (furosemide-unloaded, FU). Horses performed an incremental exercise test on a treadmill during which rates of oxygen consumption (VO2) and carbon dioxide production (VCO2) were measured, respiratory exchange ratio was calculated, and blood samples were collected for determination of mixed venous plasma lactate concentration and arterial and mixed venous oxygen saturation. Furosemide treatment caused significantly (P < 0.001) greater weight loss than did saline administration; mean +/- SEM weight loss (exclusive of fecal loss) was 1.6, 8.8, and 10.2 kg (SEM = 2.0) for C, FL, and FU trials, respectively. The speed at which peak VO2 was achieved was 9.31, 9.56, and 9.50 (SEM = 0.16) m/s, respectively, time to fatigue was 547, 544, and 553 (SEM = 26) seconds, respectively, and the highest speed attained was 10.3, 10.2, and 10.2 (SEM = 0.2) m/s, respectively. Mean peak rate of oxygen consumption was 130.7, 129.6, and 129.6 (SEM = 1.9) ml/min/kg, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210.
authors.forename: K W
authors.initials: KW
authors.lastname: Hinchcliff
authors.forename: K H
authors.initials: KH
authors.lastname: McKeever
authors.forename: W W
authors.initials: WW
authors.lastname: Muir
authors.forename: R
authors.initials: R
authors.lastname: Sams
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D005665:Furosemide / Q000494:pharmacology*; D006736:Horses / Q000378:metabolism*; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D010101:Oxygen Consumption / Q000187:drug effects; D010805:Physical Conditioning, Animal; D015431:Weight Loss / Q000187:drug effects*
chemical_list: D007773:Lactates; D002245:Carbon Dioxide; D019344:Lactic Acid; D005665:Furosemide