title: Characterization of a polyclonal antiserum against the purified human recombinant calcium binding protein calretinin.
abstract: We have purified recombinant human calretinin (CR) from Escherichia coli lysates and have produced a polyclonal antiserum against it. The antiserum recognizes determinants conserved in fish, chicken, rat, monkey and human CR. We show its use in the qualitative detection of CR by different methods of immunohistochemistry as well as in the detection of CR on immunoblots.
authors.affiliation: Institute of Histology and General Embryology, University of Fribourg, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Schwaller
authors.forename: P
authors.initials: P
authors.lastname: Buchwald
authors.forename: I
authors.initials: I
authors.lastname: Blümcke
authors.forename: M R
authors.initials: MR
authors.lastname: Celio
authors.forename: W
authors.initials: W
authors.lastname: Hunziker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D001483:Base Sequence; D001923:Brain Chemistry; D064032:Calbindin 2; D002645:Chickens; D004926:Escherichia coli; D005399:Fishes; D000882:Haplorhini; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000906:Antibodies; C576764:CALB2 protein, human; C576512:Calb2 protein, rat; D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G