Inhibition of basic fibroblast growth factor-induced growth promotion by overexpression of syndecan-1.
The expression of syndecan-1, the prototype member of the cell surface proteoglycan family, follows morphogenetic rather than histological boundaries during organ formation. As a heparan sulfate-containing cell surface molecule, syndecan-1 can simultaneously bind various components of the extracellular matrix and members of the heparin-binding growth factors. Indeed, syndecan-1 may act as a co-receptor for basic fibroblast growth factor (bFGF) (Salmivirta, M., Heino, J., and Jalkanen, M. (1992) J. Biol. Chem. 267, 17606-17610), allowing the growth factor to bind the tyrosine kinase bFGF receptor. We have studied the role of syndecan-1 in growth factor response by growing 3T3 cells transfected with syndecan-1 in the presence of bFGF. The enhanced expression of syndecan-1 caused down-regulation of bFGF-induced cell proliferation and, at the same time, enhancement of cell matrix interactions. Thus, the induced expression of the heparan sulfate co-receptor (syndecan-1) may provide a mechanism to restrict FGF action and modulate cell-matrix interactions to maintain co-ordinated growth of cells during organ formation.
Mali
M
M
Department of Medical Biochemistry, University of Turku, Finland.
Elenius
K
K
Miettinen
H M
HM
Jalkanen
M
M
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects; D017931:DNA Primers; D015536:Down-Regulation; D005109:Extracellular Matrix / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors*; D005353:Fibronectins / Q000378:metabolism; D006493:Heparin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000235:genetics* / Q000502:physiology; D053668:Syndecan-1; D053667:Syndecans; D014162:Transfection
D017931:DNA Primers; D005353:Fibronectins; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508242:Sdc1 protein, mouse; D053668:Syndecan-1; D053667:Syndecans; D016222:Fibroblast Growth Factor 2; D006493:Heparin