title: Nerve growth factor synthesis by mouse submandibular gland cells in culture.
abstract: Mouse submandibular gland (SMG) cells in culture rarely retain functional properties of SMG cells in vivo. We demonstrate that both primary SMG cells and the mouse SMG cell line SCA-9 secrete biologically active nerve growth factor (NGF). However, primary cells secrete 40-fold more NGF than SCA-9 cells, demonstrating that SCA-9 cells cannot substitute for primary SMG cells for the study of SMG NGF in vitro.
authors.affiliation: Department of Biomedical Sciences, McMaster University, Hamilton, Ont., Canada.
authors.forename: M
authors.initials: M
authors.lastname: Fahnestock
authors.forename: Q
authors.initials: Q
authors.lastname: Lu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism*
chemical_list: D009414:Nerve Growth Factors