An aminoterminally extended P1-protamine variant in the boar.
We have found a minor form of boar sperm P1-protamine with a six amino acid long N-terminal extension. When this sequence was compared with the recently published gene sequence of boar P1-protamine, and with DNA sequences obtained from the boars studied here, a perfect match was found with the 5'-region upstream of the ATG initiation codon of the authentic P1-protamine. Since no extra in-frame ATG initiation codons are present in the 5'-region of the gene, we conclude that the translation of boar P1-protamine variant starts with a non-AUG initiation codon, CUG, which precedes the codon of the first amino acid (serine) of the sequenced variant.
Pirhonen
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A
Department of Biochemistry and Biotechnology, University of Kuopio, Finland.
Jääskeläinen
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S
Linnala-Kankkunen
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A
Mäenpää
P H
PH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003062:Codon; D016384:Consensus Sequence; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011479:Protamines / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D003062:Codon; D017931:DNA Primers; D011479:Protamines; C046533:protamine P1; D004247:DNA