A gene involved in the biogenesis of c-type cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria [corrected].
Sequence analysis of a transcribed region of the wheat mitochondrial (mt) genome revealed two open reading frames (orfs) coding for proteins of 589 and 174 amino acids. Both genes are co-transcribed in a 2.6-kb RNA. The largest orf codes for a hydrophobic protein which bears similarity to a bacterial protein involved in the biogenesis of c-type cytochromes. Its corresponding RNA sequence is fully edited at 34 positions. The second orf encodes a protein homologous to the amino-terminal third of E. coli ribosomal protein S1, corresponding to the ribosome-binding domain of this protein. Its RNA sequence is edited at four positions, one of the edits creating a stop codon. The presence of both proteins in wheat mitochondria was demonstrated using specific antibodies raised against fusion proteins obtained in E. coli from the corresponding cDNAs.
Gonzalez
D H
DH
Institute de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
Bonnard
G
G
Grienenberger
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003580:Cytochromes / Q000096:biosynthesis* / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017393:RNA Editing; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics* / Q000648:ultrastructure
D003580:Cytochromes; D004272:DNA, Mitochondrial; D012269:Ribosomal Proteins; C015494:ribosomal protein S1