title: Kinesin-mediated vesicular transport in a biochemically defined assay.
abstract: Here we have described simple and reproducible methods to observe kinesin-mediated vesicle and microtubule movements under defined conditions using video microscopy. We are optimistic that this assay will provide a useful tool to study kinesin function, regulation, and dynamic physical interactions with membranous organelles and microtubules.
authors.affiliation: Laboratory of Cellular Biology, National Institute for Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: R
authors.initials: R
authors.lastname: Urrutia
authors.forename: D B
authors.initials: DB
authors.lastname: Murphy
authors.forename: B
authors.initials: B
authors.lastname: Kachar
authors.forename: M A
authors.initials: MA
authors.lastname: McNiven
mesh_terms: D000818:Animals; D002837:Chromaffin Granules / Q000502:physiology; D016547:Kinesins / Q000502:physiology*; D008870:Microtubules / Q000502:physiology; D009068:Movement; D015388:Organelles / Q000502:physiology*
chemical_list: D016547:Kinesins