A quantitative determination of singlet oxygen with horseradish peroxidase.
A singlet oxygen determination method based on the formation of dimethyl-(2,5)-2-methoxy-5-hydroperoxydihydrofuran (DMFO2) followed by acid hydrolysis with 0.1 N H2SO4 is described. Hydrogen peroxide is formed thereby as a product of hydrolysis which is then determined by the horseradish peroxidase-catalyzed formation of the homovanillic acid dimer. Since DMFO2 is a crystalline compound with sufficient stability it can be used as a primary standard for the determination. The peroxidase-catalyzed hydrogen peroxide determination allows detection of singlet oxygen concentrations as low as 10(-7) M.
Kreitner
M
M
Ludwig Boltzmann Institut für Klinische Onkologie und Photodynamik, Vienna, Austria.
Alth
G
G
Koren
H
H
Loew
S
S
Ebermann
R
R
D005609:Free Radicals; D005663:Furans / Q000737:chemistry; D006415:Hematoporphyrins / Q000737:chemistry; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D000432:Methanol / Q000737:chemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000737:chemistry; D012973:Sodium Hypochlorite / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
D005609:Free Radicals; D005663:Furans; D006415:Hematoporphyrins; D017382:Reactive Oxygen Species; D013481:Superoxides; C039316:potassium superoxide; D006861:Hydrogen Peroxide; C037555:2,5-dimethylfuran; D012973:Sodium Hypochlorite; D006735:Horseradish Peroxidase; D000432:Methanol