Simultaneous determination of creatine compounds and adenine nucleotides in myocardial tissue by high-performance liquid chromatography.
A rapid high-performance liquid chromatography method was developed for the determination of creatine phosphate, creatine, adenine nucleotides, and related compounds in myocardial tissue. Analysis was performed by reversed-phase chromatography on a C18 column containing 3-microns particles, employing gradient elution and uv detection at 210 nm. Separation was achieved in less than 5 min. Total analysis time, including equilibration of the column after return of the gradient to starting conditions, was 8 min. The high reproducibility and short analysis time make this method suitable for the routine analysis of large series of samples.
Teerlink
T
T
Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands.
Hennekes
M
M
Bussemaker
J
J
Groeneveld
J
J
D000227:Adenine Nucleotides / Q000032:analysis / Q000302:isolation & purification; D000244:Adenosine Diphosphate / Q000032:analysis; D000249:Adenosine Monophosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003401:Creatine / Q000032:analysis* / Q000302:isolation & purification; D003402:Creatine Kinase / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D009243:NAD / Q000032:analysis; D010725:Phosphocreatine / Q000032:analysis* / Q000302:isolation & purification; D013552:Swine
D000227:Adenine Nucleotides; D010725:Phosphocreatine; D009243:NAD; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D003401:Creatine