title: Charcoal stripping augments type II oestradiol binding in cytoplasmic fraction of rat hypothalami.
abstract: Type II [3H]oestradiol binding was assessed by charcoal method in the low speed cytoplasmic fraction of intact female rat hypothalami. Augmented binding was observed after charcoal pretreatment applied prior to binding assay. On the other hand, loss of low affinity oestradiol binding was demonstrated after addition of protein-free ultrafiltrates of the cytosol from hypothalami or uteri. The presence of a cytosolic inhibitor activity specific for type II oestradiol binding in hypothalami is considered.
authors.affiliation: Institute of Pathophysiology, University Medical School, Pécs/Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Garai
authors.forename: M
authors.initials: M
authors.lastname: Vértes
authors.forename: S
authors.initials: S
authors.lastname: Kovács
mesh_terms: D000818:Animals; D002606:Charcoal / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000378:metabolism
chemical_list: D002606:Charcoal; D004958:Estradiol