Metallothionein reacts with Fe2+ and NO to form products with A g = 2.039 ESR signal.
The interaction of metallothionein, Fe2+, and nitric oxide has been examined by electron spin resonance spectroscopy. When either apoMT 2 or Zn-MT 2 from rabbit liver was mixed with Fe2+ and NO, paramagnetic products with g values of 2.013 and 2.039 were found. These are characteristic of iron nitrosyl thiolates with stoichiometry, Fe(NO)2(SR)2. This product was not generated when beef heart mitochondrial aconitase was used as the source of iron.
Kennedy
M C
MC
Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226.
Gan
T
T
Antholine
W E
WE
Petering
D H
DH
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000737:chemistry* / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D011817:Rabbits; D015032:Zinc / Q000494:pharmacology
D009569:Nitric Oxide; D008668:Metallothionein; D007501:Iron; D015032:Zinc