title: An in vitro assay utilising parasitic larval Haemonchus contortus to detect resistance to closantel and other anthelmintics.
abstract: A new assay for detecting resistance to anthelmintics in vitro is described. The assay uses a simple culture system in which the ability of anthelmintics to kill or inhibit the migration of parasitic third and fourth stage Haemonchus contortus larvae through a 50 microns aperture mesh is assessed. The assay detects 2-10-fold resistance to closantel. Resistance to benzimidazoles, levamisole and ivermectin can also be detected.
authors.affiliation: Department of Veterinary Pathology, University of Sydney, New South Wales, Australia.
authors.forename: J T
authors.initials: JT
authors.lastname: Rothwell
authors.forename: N C
authors.initials: NC
authors.lastname: Sangster
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D006189:Haemonchus / Q000187:drug effects*; D007559:Ivermectin / Q000494:pharmacology; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D012458:Salicylanilides / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D000871:Anthelmintics; D012458:Salicylanilides; D007559:Ivermectin; C023342:closantel