Production and characterization of recombinant turkey prolactin.
1. Recombinant turkey prolactin (rctPRL) was produced as a fusion protein in E. coli, purified by affinity chromatography followed by cleavage with thrombin. The final yield of the released rctPRL (> 90% purity) was 1-2 mg/l of bacterial culture. 2. Recombinant tPRL co-migrated with the main immunoreactive band (25 kDa) in turkey pituitary extracts and was identical to natural tPRL except for the addition of three amino acids (Gly-Ser-Ser) resulting from the cloning strategy at the amino terminal end. 3. The bioactivity of the rctPRL was equipotent to ovine PRL in a rabbit mammary explant system and in the Nb2 lymphoma mitogenic assay.
Karatzas
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Department of Animal Science, McGill University, P.Q., Canada.
Guémené
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Zadworny
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Kühnlein
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U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002846:Chromatography, Affinity; D005815:Genetic Code; D005822:Genetic Vectors; D002479:Inclusion Bodies / Q000378:metabolism*; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000276:immunology; D011388:Prolactin / Q000032:analysis / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012995:Solubility; D013917:Thrombin / Q000378:metabolism; D014422:Turkeys / Q000378:metabolism*
D011994:Recombinant Proteins; D011388:Prolactin; D013917:Thrombin