[Inhibition of reproduction of the human immunodeficiency virus by sense and antisense oligodeoxynucleotides].
Inhibitory effects on human immunodeficiency virus (HIV) reproduction on lymphoid cell line MT-4 were characterized for antisense and sense oligodeoxynucleotides. It was established that antisense oligonucleotide pCGTAGTTCGTCGAGGTCCGT (MP-20) (ID50 = 0.1 microM) is a more effective HIV inhibitor than the previously described pTGGCGTACTCACCAGTCGCCGC (DSS-22) (ID50 = 4.7 microM) and pTTTTTTTTTTTTTTTT (PA-16) (ID50 = 8.0 microM). A sense oligonucleotide pGCATCAAGCAGCTCCAGGCA (PM-20) (ID50 = 0.5 microM) complementary to the region of the start of translation of the open reading frame on the (+)-chain virus DNA was also investigated. Specificity of the anti-HIV-I action of oligonucleotides was confirmed by experiments with HIV-II.
Pokrovskiĭ
A G
AG
Pliasunova
O A
OA
Blinov
V M
VM
Vlasov
V V
VV
Svinarchuk
F P
FP
Abramova
T V
TV
Gorn
V V
VV
Konevets
D A
DA
D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D015498:HIV-2 / Q000187:drug effects* / Q000502:physiology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D014176:Protein Biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense
Ingibirovanie reproduktsii virusa immunodefitsita cheloveka smyslovymi i antismyslovymi oligodezoksinukleotidami.