Tumor idiotype vaccines. VIII. Analysis of protective idiotype in sera and hybridomas derived from tumor-bearing mice with long-term survival.
In this study, the contribution of idiotype-positive antitumor antibodies (anti-Id) in protective tumor immunity was investigated. We have previously shown that among various anti-Id generated and typed as the internal-image Ab2 of the tumor-associated antibody (TAA) gp52, only 2F10 antibody induces protective immunity. Increase of the 2F10 idiotope in sera of tumor-bearing mice correlated with long-term survival, while in mice with short survival the circulating 2F10 idiotype decreased. 2F10+ Ig were purified from sera of tumor-bearing mice with long-term survival and the amount of 2F10+ anti-TAA antibodies was determined. Only about 3% of 2F10+ antibodies are 2F10+ anti-TAA+. Hybridomas were generated from a 2F10 high mouse with spontaneous tumor regression. Only 2 out of 52 tumor-specific hybridomas were 2F10+. These results suggest that the protective effect induced by 2F10 vaccination may not be directly mediated by 2F10+ antibodies but indirectly through the stimulation of a 2F10-specific cellular immune response.
Chen
J J
JJ
San Diego Regional Cancer Center, CA 92121.
Kohler
H
H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D016233:Immunotherapy, Active; D007939:Leukemia L1210 / Q000276:immunology* / Q000517:prevention & control; D051379:Mice; D008811:Mice, Inbred DBA; D009365:Neoplasm Regression, Spontaneous / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000912:Antibodies, Neoplasm; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M