Antigens pre-bound to the primate erythrocyte complement receptor via cross-linked bispecific monoclonal antibody heteropolymers are rapidly cleared from the circulation.
We have used cross-linked, bispecific monoclonal antibody heteropolymers, specific for primate erythrocyte (E) complement receptor (CR1) and target antigen (Ag), to attach 125I-labeled Ag to 51Cr-labeled monkey erythrocytes (E) in vitro. Injection of these sensitized E into monkeys leads to rapid clearance from the circulation of several different 125I-labeled Ag with little, if any sequestration, lysis, or clearance of the E. It should now be possible, in principle, to use the heteropolymer-E system to facilitate the clearance of blood-borne pathogens from the circulation.
Reist
C J
CJ
Department of Biochemistry, University of Virginia School of Medicine, Charlottesville 22908.
Combs
M J
MJ
Croft
B Y
BY
Taylor
R P
RP
D000818:Animals; D018033:Antibodies, Bispecific / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000097:blood*; D000936:Antigen-Antibody Complex / Q000097:blood; D000941:Antigens / Q000097:blood*; D003432:Cross-Linking Reagents; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008657:Metabolic Clearance Rate; D011951:Receptors, Complement / Q000276:immunology*
D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens; D003432:Cross-Linking Reagents; D011951:Receptors, Complement