title: Interleukin-6 production by brain tissue and cultured astrocytes infected with Theiler's murine encephalomyelitis virus.
abstract: Theiler's murine encephalomyelitis virus (TMEV) is known to interact with cells of the central nervous system (CNS). Here we report that, interestingly, it is a potent inductor of interleukin-6 (IL-6) in the CNS of infected animals and in pure cultures of astrocytes. Maximal IL-6 gene transcription in glial cells, as detected by bioassay and ELISA, was observed at 6 and 24 h after infection. Astrocytes from both SJL/J and Balb/c (strains of mice susceptible and resistant, respectively, to TMEV-induced demyelination) produced similar amounts of IL-6, measured in tissue culture supernatants. These results indicate that although an immunomodulatory effect can be exercised by IL-6 synthesized by astrocytes, it does not play a crucial role in immune-mediated demyelination induced by TMEV.
authors.affiliation: Department of Neuroimmunology, Instituto Cajal, Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Rubio
authors.forename: A
authors.initials: A
authors.lastname: Sierra
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001681:Biological Assay; D001921:Brain / Q000378:metabolism* / Q000382:microbiology; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011051:Poliomyelitis / Q000378:metabolism; D017945:Theilovirus / Q000502:physiology*
chemical_list: D015850:Interleukin-6