title: Anti-proliferative effect of combined tumour necrosis factor-alpha and interferon-alpha on human pancreatic cancer in vitro and in vivo.
abstract: The anti-proliferative effects of tumour necrosis factor-alpha (TNF-alpha) and interferon-alpha (IFN-alpha), alone or in combination, on human pancreatic cancer cells lines (PANC-1, MIA PaCa-2 and BxPC-3) and human pancreatic cancer tumour (Exp-58), were investigated in vitro and in vivo. The anti-proliferative effect was determined using the dye uptake method and the subcutaneous tumour model. Combined TNF-alpha and IFN-alpha demonstrated marked synergistic and/or additive effects in comparison with their effects as single agents. These results suggest that combined cytokine therapy of TNF-alpha and IFN-alpha may make possible some improvement in the treatment of pancreatic carcinoma patients in the future.
authors.affiliation: First Department of Surgery, Okayama University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Fuchimoto
authors.forename: H
authors.initials: H
authors.lastname: Iwagaki
authors.forename: Y
authors.initials: Y
authors.lastname: Nonaka
authors.forename: M
authors.initials: M
authors.lastname: Nezu
authors.forename: T
authors.initials: T
authors.lastname: Nishina
authors.forename: M
authors.initials: M
authors.lastname: Marutaka
authors.forename: K
authors.initials: K
authors.lastname: Susaki
authors.forename: Y
authors.initials: Y
authors.lastname: Naomoto
authors.forename: S
authors.initials: S
authors.lastname: Shiki
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016898:Interferon-alpha; D014409:Tumor Necrosis Factor-alpha