A protein with anion exchange properties found in the kidney proximal tubule.
One important mechanism for reabsorption of chloride in the kidney proximal tubule involves anion exchange of chloride for a base. Anion exchange transport systems in general demonstrate sensitivity to inhibition by disulfonic stilbenes, probenecid, furosemide, and the arginyl amino group modifier phenylglyoxal. Using disulfonic stilbene affinity chromatography, we have identified and partially purified a protein with anion exchanger properties in luminal membrane vesicles isolated from rabbit kidney cortex. This protein has a molecular weight of 162 kD. The binding of the 162 kD protein to the stilbene affinity matrix is inhibited by disulfonic stilbenes, probenecid, furosemide, and phenylglyoxal. Reconstitution of the proteins eluted from the affinity matrix into liposomes demonstrates anion exchange activity as assayed by radiolabeled chloride influx. Deletion of the 162 kD protein from the eluted mixture by probenecid diminishes the anion exchanger activity in the reconstituted liposomes. Further purification of the disulfonic stilbene column eluant by Econo-Pac Q ion exchange chromatography resulted in significant enrichment in 162 kD protein abundance and also anion exchange activity in reconstituted liposomes. The results of the above experiments strongly suggest that the 162 kD protein is an anion exchanger. Insight into the functional and molecular characteristics of this protein should provide important information about the mechanism(s) of chloride reabsorption in the kidney proximal tubule.
Soleimani
M
M
Department of Medicine, Indiana University School of Medicine, Indianapolis.
Bizal
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GL
Anderson
C C
CC
D000818:Animals; D027321:Anion Transport Proteins; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D008970:Molecular Weight; D011339:Probenecid / Q000494:pharmacology; D011510:Proteolipids; D011817:Rabbits; D013267:Stilbenes / Q000494:pharmacology
D027321:Anion Transport Proteins; D002352:Carrier Proteins; D002712:Chlorides; D011510:Proteolipids; D013267:Stilbenes; C020485:proteoliposomes; C022306:4,4'-dinitro-2,2'-stilbenedisulfonic acid; D005665:Furosemide; D011339:Probenecid