Isolation of a new species of Physarum lysophosphatidic acid, PHYLPA, and its effect on DNA polymerase activity.
A new species of lysophosphatidic acid was isolated from myxoamoebae of a true slime mold, Physarum polycephalum, and structural studies were performed. The purified substance was subjected to nuclear magnetic resonance spectroscopy (NMR), infrared spectroscopy (IR), fast atom bombardment mass spectroscopy (FAB/MS), alkaline hydrolysis and tandem mass spectroscopy (MS/MS), and the results suggested this substance to be lysophosphatidic acid composed of a cyclic phosphate and cis-11,12-methylene octadecanoic acid. The effects of the LPA on DNA polymerases were studied and compared with the effects of PHYLPA, which had been isolated as a specific inhibitor of eukaryotic DNA polymerase alpha (6). It showed a specific inhibitory activity on eukaryotic DNA polymerase alpha, but no activity on the repair-type, or mitochondrial DNA polymerases.
Takahashi
Y
Y
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan.
Shimada
Y
Y
Shioda
M
M
Yoshida
S
S
Murofushi
H
H
Murakami-Murofushi
K
K
D000818:Animals; D003498:Cyclic P-Oxides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D008246:Lysophospholipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016839:Physarum polycephalum / Q000737:chemistry*
D003498:Cyclic P-Oxides; D008246:Lysophospholipids; C084460:PHYLPA 18; D004257:DNA Polymerase II