title: Inositol 1,4,5-trisphosphate 3-kinase highest levels in the dendritic spines of cerebellar Purkinje cells and hippocampal CA1 pyramidal cells. A pre- and post-embedding immunoelectron microscopic study.
abstract: Inositol 1,4,5-trisphosphate 3-kinase (InsP3 3-kinase) plays a crucial role in calcium homeostasis by regulating InsP3 levels. We have reported the highest concentrations of InsP3 3-kinase in the dendrites of cerebellar Purkinje cells and hippocampal pyramidal cells of the CA1 sector of the Ammon's horn. We here investigate its subcellular localization by pre- and post-embedding immunoelectron microscopic study. In both populations of neurons, the major structure expressing a high level of InsP3 3-kinase is the dendritic spines.
authors.affiliation: Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Go
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: K
authors.initials: K
authors.lastname: Takazawa
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: C
authors.initials: C
authors.lastname: Erneux
authors.forename: P
authors.initials: P
authors.lastname: Mailleux
authors.forename: T
authors.initials: T
authors.lastname: Onaya
mesh_terms: D000818:Animals; D003712:Dendrites / Q000201:enzymology*; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology*; D008297:Male; D016253:Microscopy, Immunoelectron; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011689:Purkinje Cells / Q000201:enzymology*; D017966:Pyramidal Cells / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D016610:Tissue Embedding
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase