A strain-specific cyclin homolog in the fungal phytopathogen Colletotrichum gloeosporioides.
The fungus, Colletotrichum gloeosporioides, which infects the tropical pasture legume, Stylosanthes guianensis, contains highly variable mini-chromosomes. The transcription of strain-specific genomic DNA clones previously isolated from one variable mini-chromosome was investigated by using these clones to screen a cDNA library prepared from the fungus grown in liquid medium. A cDNA clone was obtained with one of the genomic clones and was sequenced. A single long open reading frame of 259 amino acids (aa) was detected with significant homology to cyclin proteins in other organisms. Northern blot analysis indicated that the cDNA corresponded to a low-abundance mRNA (approximately 0.001% of poly(A)+RNA). Southern blot analysis indicated that genes encoding this mRNA were discontinuously distributed in this fungal species, indicating it encodes a dispensable function. This result suggests that natural populations of fungi may have variable complements of cyclin-encoding genes.
Masel
A M
AM
Cooperative Research Centre for Tropical Plant Pathology, University of Queensland, Brisbane, Australia.
Struijk
N
N
McIntyre
C L
CL
Irwin
J A
JA
Manners
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D016213:Cyclins / Q000235:genetics*; D004271:DNA, Fungal; D005656:Fungal Proteins; D016681:Genome, Fungal; D003904:Mitosporic Fungi / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014158:Transcription, Genetic
C084272:CYC1 protein, Colletotrichum gloeosporioides; D018797:Cell Cycle Proteins; D016213:Cyclins; D004271:DNA, Fungal; D005656:Fungal Proteins