title: Cloning and sequence analysis of the gene encoding the crystalline surface layer protein of Rickettsia typhi.
abstract: The nucleotide sequence of the gene (slpT) encoding the crystalline surface layer protein (SLP) of Rickettsia typhi was determined. The slpT gene consists of 4935 bp coding for a 1645-amino-acid (aa) protein containing a predicted signal peptide at the N terminus. The size of the predicted SLP exceeds the observed size (135 kDa) on SDS-PAGE. The N-terminal aa sequence of the 32-kDa protein of R. typhi reported by Hackstadt et al. [Infect. Immun. 60 (1992) 159-165] was found in the C-terminal portion of the deduced aa sequence, suggesting that the product of slpT is processed into the mature SLP and the 32-kDa protein.
authors.affiliation: Department of Microbiology, Seoul National University College of Medicine, Korea.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hahn
authors.forename: K K
authors.initials: KK
authors.lastname: Kim
authors.forename: I
authors.initials: I
authors.lastname: Kim
authors.forename: W H
authors.initials: WH
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012286:Rickettsia typhi / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008562:Membrane Glycoproteins; C055764:S-layer proteins