title: Measurement of 24-h gastric pH using an indwelling pH electrode in horses unfed, fed and treated with ranitidine.
abstract: A glass combined pH-reference electrode was placed in the stomachs of 5 adult horses and pH was recorded every 6 mins for 24 h while (1) feed and all bedding materials were withheld for 24 h (unfed), (2) horses had free access to Timothy grass hay for 24 h (fed), and (3) horses had free access to Timothy grass hay and were treated with ranitidine, 6.6 mg/kg body weight, orally, every 8 h for 48 h (fed + ranitidine). There was a significant (P = 0.007) difference in median 24-h gastric pH amongst the 3 protocols, the value being 3.1 in fed horses and 1.55 in unfed horses (P = 0.05) and 4.6 in fed + ranitidine horses (P = 0.05 compared with fed horses). The percentage of pH readings < 2.0 was significantly greater (P = 0.05) in unfed (76%) than in fed (30%) horses, and in fed horses than in fed + ranitidine (9%) horses. The percentage of readings > 2.0, 3.0, 4.0, 5.0 and 6.0 were each significantly (P = 0.05) greater in fed + ranitidine horses than in fed horses, and in fed than in unfed horses. There was no difference (P = 0.13) between median gastric pH values from different times of day in unfed horses (00:00 to 06:00 h, 1.5; 06:00 to 12:00 h, 1.6; 12:00 to 18:00 h, 1.7; and 18:00 to 24:00 h, 1.5). We conclude that gastric acidity is greatest when horses do not have access to feed material and that ranitidine effectively suppresses gastric acidity in horses under conditions of free access to hay.
authors.affiliation: Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Leesburg 22075.
authors.forename: M J
authors.initials: MJ
authors.lastname: Murray
authors.forename: G F
authors.initials: GF
authors.lastname: Schusser
mesh_terms: D000818:Animals; D004435:Eating / Q000502:physiology*; D004566:Electrodes / Q000662:veterinary; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D005745:Gastric Acidity Determination / Q000662:veterinary*; D005773:Gastroscopy / Q000662:veterinary; D006736:Horses / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007441:Intubation, Gastrointestinal / Q000662:veterinary; D008297:Male; D011899:Ranitidine / Q000494:pharmacology*; D013270:Stomach / Q000737:chemistry* / Q000187:drug effects
chemical_list: D011899:Ranitidine