DNA cleavage by benzidine derivatives in weak acidic conditions.
A plasmid DNA was cleaved by aromatic amines in weak acidic conditions at 37 degrees C. The cleavage was not inhibited by 4 hydroxylradical scavengers, EDTA, SOD and catalase. It was strongly influenced by the pH value of the reaction buffer. The decrease of Form I DNA in this reaction obeyed good first-order kinetics.
Yamashita
R
R
Department of Biological Science and Technology, Science University of Tokyo, Chiba, Japan.
Namihira
T
T
Nakamura
Y
Y
D000588:Amines / Q000737:chemistry; D001560:Benzidines / Q000737:chemistry*; D002374:Catalase / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D016166:Free Radical Scavengers; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000737:chemistry; D007700:Kinetics; D010957:Plasmids; D013482:Superoxide Dismutase / Q000737:chemistry
D000588:Amines; D001560:Benzidines; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical; D004247:DNA; D004492:Edetic Acid; D002374:Catalase; D013482:Superoxide Dismutase