A new infectious mammary tumor virus in the milk of mice implanted with C4 hyperplastic alveolar nodules.
We have previously characterized an infectious mouse mammary tumor virus [(MMTV(SW)] which induces a strong superantigen response in vivo. Here we describe the isolation and characterization of MMTV(C4) which was derived from milk of mice implanted with hyperplastic alveolar nodules. MMTV(C4) stimulates V beta 2 expressing T cells after local injection in vivo. Comparison with known open reading frame (orf) sequences revealed high homology to Mtv-6, an endogenous virus interacting with V beta 3-expressing T cells. The carboxyl-terminal amino acids were, however, altered. High homology including the carboxyl-terminal orf amino acids were found with MMTV(C3H-K). We show here that MMTV(C3H-K) has lost its superantigen function. Sequence comparisons permitted the characterization of few key amino acids which could be important for T cell receptor interaction and superantigen processing.
Shakhov
A N
AN
Ludwig Institute for Cancer Research, Epalinges, Switzerland.
Wang
H
H
Acha-Orbea
H
H
Pauley
R J
RJ
Wei
W Z
WZ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006965:Hyperplasia; D008321:Mammary Glands, Animal / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D011230:Precancerous Conditions / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014764:Viral Proteins