Expression of ras-P21 and ras gene alteration in pleomorphic adenomas.
The expression of ras-P21 protein and gene alteration of the K-ras gene were examined in 12 cases of pleomorphic adenoma of the salivary gland. The tumor cells in all 12 cases were strongly stained for ras-P21 protein by the indirect immunoperoxidase method, suggesting the enhanced expression of ras-P21 protein in pleomorphic adenomas. Analysis of the PCR products for the K-ras gene by temperature-gradient gel electrophoresis (TGGE) revealed that gene alterations such as point mutations occurred in 4 out of 12 pleomorphic adenoma tissues examined.
Okutsu
S
S
Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo, Japan.
Takeda
A
A
Suzuki
T
T
Nakajima
Y
Y
Sato
J
J
Suda
K
K
Fukuda
M
M
Usami
T
T
Himiya
T
T
Kusama
K
K
D008949:Adenoma, Pleomorphic / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D015689:Oncogene Protein p21(ras) / Q000032:analysis / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D015689:Oncogene Protein p21(ras)