Silicon-responsive cDNA clones isolated from the marine diatom Cylindrotheca fusiformis.
In organisms ranging from single-celled algae to mammals, including humans, silicon is essential for, and actively participates in, a variety of life processes. It has become clear that silicon (i) acts as a metabolite affecting a variety of cellular processes, and (ii) regulates gene expression. However, the mechanisms by which silicon (i.e., Na2SiO3.9H2O, in the present study) acts are not clear, due to inherent methodological difficulties. As part of our program to understand how silicon acts in biological systems, we present the first isolation of cDNA clones derived from silicon-responsive mRNAs, from the marine diatom Cylindrotheca fusiformis. We distinguish between clones responding only to silicon starvation and replenishment, and those also responding to other cellular conditions. Some of the clones can be identified by similarity to other genes, and should be useful as probes to isolate genes from other organisms. Isolation of these clones provides the means to (i) identify metabolic pathways affected by silicon, and (ii) investigate the mechanism(s) of silicon-regulated gene expression.
Hildebrand
M
M
Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
Higgins
D R
DR
Busser
K
K
Volcani
B E
BE
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017377:Diatoms / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D012333:RNA, Messenger / Q000378:metabolism; D012825:Silicon / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D012825:Silicon