title: Poly (adenosine diphosphate-ribose) synthesis in the anterior pituitary of the female rat throughout the estrous cycle: study of possible relation to cell proliferation and prolactin gene expression.
abstract: To investigate whether cell proliferation and PRL gene expression in female rat pituitary during estrous cycle were mediated by the poly (ADP)-ribosylation of chromatin proteins, anterior pituitaries at different estrous cycle were obtained from female Wistar rats, and poly (ADP-ribose) synthesis, DNA synthesis, PRL and GH messenger RNAs and PRL content in the pituitary, and serum concentrations of PRL and estradiol were analyzed. From diestrus to proestrus, poly (ADP-ribose) synthesis, the contents of PRL messenger RNA and PRL in the pituitary increased significantly, and decreased at estrus. However, DNA synthesis and serum concentration of PRL showed a significant increase from proestrus to estrus. Serum estradiol concentration increased from diestrus to proestrus. No significant change was observed in the pituitary GH messenger RNA content during estrous cycle. The increase of PRL messenger RNA from diestrus to proestrus was abolished completely by the administration of nicotinamide, an inhibitor of poly (ADP-ribose) synthesis, to rats at diestrus. These results indicate that poly (ADP)-ribosylation of chromatin proteins may play some role in cell proliferation and transcription of PRL gene during rat estrous cycle.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Nagoya University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Suganuma
authors.forename: F
authors.initials: F
authors.lastname: Kikkawa
authors.forename: H
authors.initials: H
authors.lastname: Seo
authors.forename: N
authors.initials: N
authors.lastname: Matsui
authors.forename: Y
authors.initials: Y
authors.lastname: Tomoda
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004971:Estrus / Q000097:blood / Q000378:metabolism*; D005260:Female; D013006:Growth Hormone / Q000096:biosynthesis; D009536:Niacinamide / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D011388:Prolactin / Q000096:biosynthesis* / Q000097:blood / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000097:blood / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012333:RNA, Messenger; D009536:Niacinamide; D011064:Poly Adenosine Diphosphate Ribose; D011388:Prolactin; D013006:Growth Hormone; D004247:DNA