title: Misincorporation of ribonucleotides by DNA polymerase during in vitro DNA replication.
abstract: The fidelity of DNA replication with respect to misincorporation of ribonucleotides into DNA has been studied. Primed M13 DNA was replicated by E. coli DNA polymerase I in the presence of four dNTPs, a single rNTP and Mg2+ as a cofactor. All four rNTPs were incorporated into DNA in place of the corresponding dNTPs when rNTP/dNTP ratio was 1000:1. The frequency of rNTP misincorporation was C > G > A > U.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ide
authors.forename: R
authors.initials: R
authors.lastname: Yagi
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D004256:DNA Polymerase I / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D012265:Ribonucleotides / Q000378:metabolism*; D013698:Templates, Genetic
chemical_list: D004279:DNA, Viral; D012265:Ribonucleotides; D004256:DNA Polymerase I