Isolation of prolactin and growth hormone from the pituitary of the holostean fish Amia calva.
Pituitaries from adult male and female Amia calva (Order Holostei) were acid extracted and fractionated by gel filtration column chromatography and reversed-phase high performance liquid chromatography. This two-step isolation procedure yielded homogeneous pools of Amia prolaction (PRL) and growth hormone (GH). The amino acid composition of both purified polypeptides was determined. Primary sequence analysis of the first 22 positions at the N-terminal of Amia PRL revealed that this region has 63% sequence identity with eel PRL-1. The N-terminal region of Amia PRL lacks the disulfide bridge which is characteristic of tetrapod PRLs. Primary sequence analysis of the first 24 positions at the N-terminal of Amia GH revealed that this region has 62% sequence identity with eel GH and 54% sequence identity with both blue shark GH and sea turtle GH. Based on N-terminal analysis, it appears that Amia PRL and GH are more closely related to teleost PRLs and GHs than they are to tetrapod PRLs and GHs.
Dores
R M
RM
University of Denver, Department of Biological Sciences, CO 80208.
Noso
T
T
Rand-Weaver
M
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Kawauchi
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H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004524:Eels; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005399:Fishes / Q000378:metabolism*; D013006:Growth Hormone / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry* / Q000378:metabolism; D011388:Prolactin / Q000302:isolation & purification* / Q000378:metabolism; D012474:Salmon; D017421:Sequence Analysis; D012754:Sharks; D014426:Turtles
D000596:Amino Acids; D011388:Prolactin; D013006:Growth Hormone