Dauricine and anisodamine inhibited leukotrienes- and platelet activating factor-induced DNA synthesis and proliferation of bovine cerebral microvascular smooth muscle cells in culture.
The effects of leukotrienes (LT) and platelet activating factor (PAF) on DNA synthesis and proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC) were studied. At 100 pmol.L-1, LTB4, LTC4, LTD4, and PAF promoted the DNA synthesis by 44%, 50%, 48%, and 57%, and enhanced the cell proliferation by 33%, 47%, 27%, and 40%, respectively. Dauricine and anisodamine inhibited the DNA synthesis of the cells induced by LT and PAF (0.1-100 mumol.L-1). These results indicate the bright future of the 2 drugs in the prevention and treatment of cerebral vascular diseases.
Zeng
G Q
GQ
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China.
Ju
D W
DW
Sun
D X
DX
Rui
Y C
YC
D000470:Alkaloids; D000818:Animals; D044182:Benzylisoquinolines; D001921:Brain / Q000098:blood supply; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D007546:Isoquinolines / Q000494:pharmacology*; D020024:Leukotriene Antagonists; D008833:Microcirculation; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D012991:Solanaceous Alkaloids / Q000494:pharmacology*; D044005:Tetrahydroisoquinolines
D000470:Alkaloids; D044182:Benzylisoquinolines; D007546:Isoquinolines; D020024:Leukotriene Antagonists; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D012991:Solanaceous Alkaloids; D044005:Tetrahydroisoquinolines; C003922:anisodamine; C035934:dauricine; D004247:DNA