Cloning, nucleotide sequence and expression of a beta-lactamase gene from Streptomyces lavendulae.
A hybridized DNA fragment was cloned as a 7.6-kb fragment from Streptomyces lavendulae KCCS0263 using a 1.9-kb SacI-XbaI DNA fragment from S. cellulosae as a probe. The latter fragment encoded a beta-lactamase which can bind blue dextran. The hybridized region was reduced to a 2.8-kb KpnI-BclI fragment and the nucleotide sequence was determined. The nucleotide sequence indicated one open reading frame whose amino acid sequence is very similar to that of the beta-lactamase from S. cellulosae. The gene produced beta-lactamase enzyme at a low but significant amount.
Sendouda
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Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan.
Urabe
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Ogawara
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000145:classification / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases