title: Evidence for release of free glucose from muscle during amylin-induced glycogenolysis in rats.
abstract: Amylin, a 37 amino acid product of pancreatic beta-cells, inhibits insulin-stimulated radioglucose incorporation into muscle glycogen. In the current study, we exercised rats and then prelabelled the glycogen pool by infusing [3-3H]glucose along with glucose and insulin. Subsequent amylin administration increased the rate of appearance of tritiated water 6.4-fold, consistent with stimulation of glycogenolysis and passage of the released moieties through the hexose --> triose step of glycolysis. Further, there was an increase in plasma [3-3H]glucose after amylin, consistent with the release of free glucose previously sequestered in muscle glycogen. Calcitonin gene-related peptide (8-37), an amylin antagonist, prevented these actions.
authors.affiliation: Department of Physiology, Amylin Pharmaceuticals Inc., San Diego, CA 92121.
authors.forename: A A
authors.initials: AA
authors.lastname: Young
authors.forename: P
authors.initials: P
authors.lastname: Carlo
authors.forename: P
authors.initials: P
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Wolfe-Lopez
authors.forename: R
authors.initials: R
authors.lastname: Pittner
authors.forename: M W
authors.initials: MW
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Rink
mesh_terms: D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D058228:Islet Amyloid Polypeptide; D008297:Male; D009132:Muscles / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D006003:Glycogen; D005947:Glucose