title: Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.
abstract: The expression at different temperatures of the lacZ gene, which is controlled by the lambda pL and pR tandem promoters and the cI857 temperature-sensitive repressor, was studied in Escherichia coli continuous cultures. At temperatures between 30 and 42 degrees C, beta-galactosidase activity behaved according to an exponential equation. By inducing a culture at a temperature within this range, predefined, nearly constant submaximal levels of gene expression and recombinant product yield can be obtained.
authors.affiliation: Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Villaverde
authors.forename: A
authors.initials: A
authors.lastname: Benito
authors.forename: E
authors.initials: E
authors.lastname: Viaplana
authors.forename: R
authors.initials: R
authors.lastname: Cubarsi
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics; D013696:Temperature; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D012097:Repressor Proteins; D001616:beta-Galactosidase