Mapping of a gene for familial juvenile nephronophthisis: refining the map and defining flanking markers on chromosome 2. APN Study Group.
Familial juvenile nephronophthisis (NPH) is an autosomal recessive kidney disease that leads to end-stage renal failure in adolescence and is associated with the formation of cysts at the cortico-medullary junction of the kidneys. NPH is responsible for about 15% of end-stage renal disease in children, as shown by Kleinknecht and Habib. NPH in combination with autosomal recessive retinitis pigmentosa is known as the Senior-Løken syndrome (SLS) and exhibits renal pathology that is identical to NPH. We had excluded 40% of the human genome from linkage with a disease locus for NPH or SLS when Antignac et al. first demonstrated linkage for an NPH locus on chromosome 2. We present confirmation of linkage of an NPH locus to microsatellite markers on chromosome 2 in nine families with NPH. By linkage analysis with marker AFM262xb5 at locus D2S176, a maximum lod score of 5.05 at a theta max = .03 was obtained. In a large NPH family that yielded at D2S176 a maximum lod score of 2.66 at theta max = .0, markers AFM172xc3 and AFM016yc5, representing loci D2S135 and D2S110, respectively, were identified as flanking markers, thereby defining the interval for an NPH locus to a region of approximately 15 cM. Furthermore, the cytogenetic assignment of the NPH region was specified to 2p12-(2q13 or adjacent bands) by calculation of linkage between these flanking markers and markers with known unique cytogenetic assignment. The refined map may serve as a genetic framework for additional genetic and physical mapping of the region.
Hildebrandt
F
F
University Children's Hospital, Freiburg, Germany.
Singh-Sawhney
I
I
Schnieders
B
B
Centofante
L
L
Omran
H
H
Pohlmann
A
A
Schmaltz
C
C
Wedekind
H
H
Schubotz
C
C
Antignac
C
C
D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D004276:DNA, Satellite; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006239:Haplotypes; D006801:Humans; D007674:Kidney Diseases / Q000235:genetics*; D052177:Kidney Diseases, Cystic / Q000235:genetics; D007676:Kidney Failure, Chronic / Q000235:genetics; D008297:Male; D010375:Pedigree
D004276:DNA, Satellite; D005819:Genetic Markers