Improved method to extract and concentrate porphyrins from liver tissue for analysis by high-performance liquid chromatography.
A new method to extract and concentrate porphyrins from liver tissue for analysis by high-performance liquid chromatography is described. Porphyrins were extracted with acetonitrile-hydrochloric acid, concentrated on disposable octadecylsilyl columns, brought to dryness, and reconstituted in 1.2 M hydrochloric acid. Dried porphyrins were stable at -80 degrees C for at least three weeks, and porphyrins reconstituted in hydrochloric acid were stable for at least 20 h. Yields of uro-, heptacarboxyl-, hexacarboxyl-, pentacarboxyl- and coproporphyrin were > or = 90%. The method allows determination of porphyrin concentrations in small (10-100 mg) samples of liver tissue.
Kennedy
S W
SW
Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Quebec.
James
C A
CA
D000097:Acetonitriles; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D006851:Hydrochloric Acid; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry*; D011166:Porphyrins / Q000032:analysis* / Q000302:isolation & purification
D000097:Acetonitriles; D007202:Indicators and Reagents; D011166:Porphyrins; D006851:Hydrochloric Acid