title: Lyophilization can generate artifacts in chromatographic profiles of inositol phosphates.
abstract: Lyophilized extracts of [3H]inositol-labelled rat heart or renal tubule preparations were found to contain unidentified 3H-labelled compounds in addition to the inositol phosphates. The appearance of these labelled substances was caused by the presence in the extracts of compounds which bound [3H]inositol when lyophilized together with it. These studies demonstrate a previously undescribed source of [3H]inositol-labelled compounds which can complicate chromatographic profiles of inositol phosphates. These problems can be overcome either by not lyophilizing the samples or by lyophilizing in the presence of 0.3 M urea, which prevents the association with [3H]inositol and does not interfere with the chromatography.
authors.affiliation: Cellular Biochemistry Laboratory, Baker Medical Research Institute, Prahran, Australia.
authors.forename: E A
authors.initials: EA
authors.lastname: Woodcock
authors.forename: K E
authors.initials: KE
authors.lastname: Anderson
authors.forename: S L
authors.initials: SL
authors.lastname: Land
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005612:Freeze Drying; D007295:Inositol Phosphates / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D007679:Kidney Medulla / Q000737:chemistry; D008297:Male; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014508:Urea
chemical_list: D007295:Inositol Phosphates; D014508:Urea