title: Determination of ivermectin residues in the tissues of Atlantic salmon (Salmo salar) using HPLC with fluorescence detection.
abstract: A sensitive, accurate and reproducible assay for the determination of residual concentrations of ivermectin in the tissues of farmed Atlantic salmon is described. The assay employs the clean-up of an acetonitrile extract of salmon tissue on a Bond-Elut C8 column, followed by derivatization with trifluoroacetic acid anhydride. Following separation using HPLC, 22,23-dihydroavermectin B1a is determined using fluorescence detection. The limit of detection of the assay is 1 ng per g tissue. Liver, taken from fish treated experimentally with ivermectin, was found to contain higher concentrations of 22,23-dihydroavermectin B1a than any other tissue examined. The half-life of 22,23-dihydroavermectin B1a was similar in all tissues studied, ranging from 89 to 98 degree days.
authors.affiliation: Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UK.
authors.forename: D G
authors.initials: DG
authors.lastname: Kennedy
authors.forename: A
authors.initials: A
authors.lastname: Cannavan
authors.forename: S A
authors.initials: SA
authors.lastname: Hewitt
authors.forename: D A
authors.initials: DA
authors.lastname: Rice
authors.forename: W J
authors.initials: WJ
authors.lastname: Blanchflower
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis; D007559:Ivermectin / Q000032:analysis* / Q000493:pharmacokinetics; D012474:Salmon / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D007559:Ivermectin