Molecular cloning and mapping of 16S-23S rRNA gene complexes of Staphylococcus aureus.
Staphylococcus aureus BB255, a derivative of NCTC8325, had six rRNA operons, and each operon contained two SmaI sites about 3 kb apart. By molecular cloning and pulsed-field gel electrophoresis, all operons were mapped at the junctions of SmaI fragments in the published map of NCTC8325 except one, which was connected to a previously unidentified 23-kb SmaI fragment.
Wada
A
A
Department of Bacteriology, Juntendo University, Tokyo, Japan.
Ohta
H
H
Kulthanan
K
K
Hiramatsu
K
K
D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000235:genetics*
D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S