Effect of prostaglandins and superoxide dismutase administration on oxygen free radical production in experimental acute pancreatitis.
Oxygen free radicals and prostaglandins are implicated in the pathophysiology of acute pancreatitis, although their mechanisms of action remain unclear. We have studied the effect of administration of exogenous 16,16-dimethyl prostaglandin E2 and superoxide dismutase on oxygen free radical production in acute pancreatitis. For this purpose, five experimental rat groups were studied: group I, control; group II, sodium taurocholate-induced acute pancreatitis; group III, same as group II but with previous administration of 16,16-dimethyl prostaglandin E2; group IV, same as group II but with previous administration of indomethacin; and group V, same as group II but with previous administration of superoxide dismutase. In sodium taurocholate-treated rats, xanthine dehydrogenase is completely converted to xanthine oxidase within the first 5 min with subsequent oxygen free radical production while in 16,16-dimethyl prostaglandin E2-treated rats this enzyme transformation does not occur. In the superoxide dismutase-treated group xanthine oxidase activation is partially prevented. These data suggest that xanthine oxidase is the main source of oxygen free radicals, which contribute to extending the cellular damage in sodium taurocholate-induced acute pancreatitis.
Closa
D
D
Molecular Pathology Unit, CID-CSIC, Barcelona, Spain.
Bulbena
O
O
Rosello-Catafau
J
J
Fernandez-Cruz
L
L
Gelpi
E
E
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology / Q000627:therapeutic use; D000208:Acute Disease; D000818:Animals; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D004789:Enzyme Activation; D005609:Free Radicals; D007213:Indomethacin / Q000494:pharmacology / Q000627:therapeutic use; D008049:Lipase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000494:pharmacology* / Q000627:therapeutic use; D013656:Taurocholic Acid / Q000633:toxicity; D014968:Xanthine Dehydrogenase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism*
D005609:Free Radicals; D017382:Reactive Oxygen Species; D013656:Taurocholic Acid; D013482:Superoxide Dismutase; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase; D008049:Lipase; D015064:16,16-Dimethylprostaglandin E2; D007213:Indomethacin