Detection of K-ras codon 12 mutation by polymerase chain reaction and differential dot-blot hybridization in sodium saccharin-treated human RSA cell.
Using polymerase chain reaction and differential dot-blot hybridization, point mutations of K-ras codon 12 were investigated in hyper-mutable human RSa cells treated with sodium saccharin. Cells carried mutational changes after 6 days of treatment at sodium saccharin concentrations of 15 to 30 mg/ml. Results imply that sodium saccharin is a mutagen which induces genetic mutation and, furthermore, that the assay system applied here will be beneficial in identifying mutagen-induced K-ras oncogene mutations in human cells in vitro.
Suzuki
H
H
Department of Toxicology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Suzuki
N
N
D001483:Base Sequence; D002460:Cell Line; D003062:Codon / Q000235:genetics; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D011905:Genes, ras / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D012439:Saccharin / Q000633:toxicity*
D003062:Codon; D017931:DNA Primers; D015345:Oligonucleotide Probes; D012439:Saccharin