[The evaluation of the cytotoxicity of 2 dental adhesives using human pulp cells in culture].
This in vitro study was designed to ascertain the cytotoxicity of two new dentin systems, Scotchbond MP (3M) and A.R.T. Bond (Coltène), on cultured cell monolayers prepared from fresh explants of human pulp tissue and placed in contact with the materials according to two methods. In a direct method, the two components (primer, adhesive) of each bonding system were placed directly on the cell layers. In an indirect method the bonding agents were placed on dentin slices of 0.3 and 1.0 mm thickness, that were interposed between agents and cells. After 8 days the cytotoxicity was quantitatively assessed by counting of the remaining living cells. The results indicate that the different primer solutions, when placed in direct contact with the cells, are more cytotoxic than the adhesive resins. In indirect contact through dentin, both bonding systems exhibited a similar cytotoxicity. The cytotoxicity appeared to be considerably diminished with a thick dentin slice interposed. These data confirm the role of dentin as a diffusion and buffer membrane for bonding materials. This kind of experiment could in the future improve the correlation between in vitro and in vivo results.
Bouillaguet
S
S
Département de Prévention et Thérapeutique, Section de Médecine dentaire, Faculté de Médecine, Université de Genève.
Ciucchi
B
B
Holz
J
J
D002452:Cell Count / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D006801:Humans; D013997:Time Factors
D017220:Dentin-Bonding Agents
Evaluation de la cytotoxicité de deux adhésifs dentinaires à l'aide de cellules pulpaires humaines en culture.