Specific binding of [125I]iodomelatonin in pigeon and quail spleen membrane preparations and effect with hydrocortisone-treatment.
[125I]Iodomelatonin binding sites were studied in the spleens of pigeon and quail. Scatchard analyses of the membrane preparations collected at midday (12:00) revealed an equilibrium dissociation constant (Kd) of 250 +/- 74 pmol.L-1 and a maximal number of binding sites (Bmax) of 13.4 +/- 1.8 fmol/mg protein in the pigeon spleen and Kd = 390 +/- 132 pmol.L-1, Bmax = 21.3 +/- 3.7 fmol/mg protein in the quail spleen. Circadian studies indicated that spleens collected at midnight (24:00) had 53% and 70.9% less [125I]iodomelatonin binding sites than the samples collected at midday in the pigeon and quail, respectively. Specificity studies showed only melatonin and 6-chloromelatonin had significant inhibition to [125I]iodomelatonin binding. Hydrocortisone 15 mg.kg-1 x d-1 im for 5 d selectively increased [125I] iodomelatonin binding sites in pigeon spleen. These results suggest that spleen is a site of melatonin action outside the brain.
Wang
X L
XL
Department of Physiology, University of Hong Kong.
Yuan
H
H
Pang
S F
SF
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D002940:Circadian Rhythm; D010856:Columbidae; D006854:Hydrocortisone / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D008550:Melatonin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D011784:Quail; D013154:Spleen / Q000378:metabolism*
D007457:Iodine Radioisotopes; C024100:6-chloromelatonin; C044556:2-iodomelatonin; D008550:Melatonin; D006854:Hydrocortisone