The effects of cytoskeletal inhibitors on the collagen gel contraction by dog periodontal ligament fibroblasts in vitro.
Periodontal ligament fibroblasts (PLF) were incorporated into small collagen gel discs (100 microliters, dia 10.0 mm), cultured in media containing various concentrations of cytochalasin D or colchicine and the diameters of the discs then measured. Cytochalasin D (3.1-800 nM) inhibited contraction of the gel discs in a dose-dependent manner and 800 nM of cytochalasin D completely inhibited contraction. This complete inhibition was still observed when cell density in the gel was increased. Although colchicine at a concentration of 0.98-15.7 nM also inhibited the contraction in a dose-dependent manner, contraction at 50% of the control level was still observed in the presence of a high concentration (0.25 mM, 100 micrograms/ml). These results strongly indicate that actin filaments are more intimately involved in PLF-mediated collagen gel contraction than microtubules. Although it is suggested that PLF are involved in tooth eruption, earlier studies have shown that microtubule inhibitors inhibit eruption of rat incisors whereas the microfilament inhibitor, cytochalasin B, has no effect. Thus, the PLF theory alone seems insufficient to explain the mechanism of tooth eruption.
Kasugai
S
S
Department of Pharmacology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Ogura
H
H
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology; D000199:Actins / Q000037:antagonists & inhibitors; D000818:Animals; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000037:antagonists & inhibitors* / Q000502:physiology; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008868:Microtubule Proteins / Q000037:antagonists & inhibitors / Q000502:physiology; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014078:Tooth Eruption / Q000187:drug effects / Q000502:physiology*
D000199:Actins; D003598:Cytoskeletal Proteins; D008868:Microtubule Proteins; D015638:Cytochalasin D; D003094:Collagen; D003078:Colchicine