title: Regional differences in the polypeptide composition of pig lens intracellular matrix and plasma membrane.
abstract: Analysis of pig lens urea-soluble fractions (USF) by gel filtration and SDS-PAGE revealed that the cortical USF is characterized by a high content of noncrystallin bands of 42, 44, 52, 55, 100 and 200 kD. These bands are markedly decreased in the nuclear USF though the 44-kD band to a lesser extent. These results suggested that pig lens nucleus contains much less cytoskeletal proteins than the cortex. Analysis of pig lens membrane showed that MP26 and MP17 are the major intrinsic membrane proteins and the relative amount of alpha A and beta B1b chains attached to the membrane are more abundant in the nucleus than in the cortex.
authors.affiliation: Department of Biochemistry, Xuzhou Medical College, Jiangsu, PROC.
authors.forename: H R
authors.initials: HR
authors.lastname: Zhao
authors.forename: X H
authors.initials: XH
authors.lastname: Ren
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D003459:Crystallins / Q000032:analysis*; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003599:Cytoskeleton / Q000737:chemistry; D007904:Lens Cortex, Crystalline / Q000737:chemistry*; D007907:Lens Nucleus, Crystalline / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D013552:Swine
chemical_list: D003459:Crystallins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D010455:Peptides