title: The V kappa genes of the L regions and the repertoire of V kappa gene sequences in the human germ line.
abstract: Only 14 of the 25 V kappa genes and pseudogenes had been found before as parts of the L regions. The cloning and linking described in the accompanying report allowed us now to assign to Lp or Ld some V kappa genes which had been found before on scattered clones. In addition the sequences of several still unknown genes are reported here, thus completing the publication of the V kappa genes of the kappa locus as far as they are potentially functional or have only one or two 1-bp defects. Of the V kappa genes of the kappa locus, 32 are potentially functional, 16 have minor defects, 3 have both potentially functional and slightly defective alleles and 25 are pseudogenes which amounts to a repertoire of 76 V kappa-related gene sequences. The V kappa genes of the L regions are, within the subgroups, particularly similar to each other, which is in part due to common evolutionary origins and in part caused by gene conversion-like events. One donor-acceptor pair could be clearly identified, since converted and not-converted alleles of the acceptor gene were found. In other cases the duplicates of the converted genes served as non-converted controls.
authors.affiliation: Institut für Physiologische Chemie der Universität München, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Huber
authors.forename: K F
authors.initials: KF
authors.lastname: Schäble
authors.forename: E
authors.initials: E
authors.lastname: Huber
authors.forename: R
authors.initials: R
authors.lastname: Klein
authors.forename: A
authors.initials: A
authors.lastname: Meindl
authors.forename: R
authors.initials: R
authors.lastname: Thiebe
authors.forename: R
authors.initials: R
authors.lastname: Lamm
authors.forename: H G
authors.initials: HG
authors.lastname: Zachau
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000916:Antibody Diversity; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000235:genetics; D005785:Gene Conversion; D005803:Genes, Immunoglobulin; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping
chemical_list: D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D004247:DNA