Precise analyses of DNA structure by NMR.
Novel 1H NMR techniques were developed and applied to the analyses of the DNA structure. They distinguished the base proton signals of cytosine (uracil) from those of the other bases. Two dimensional experiments were also performed by using these techniques, and were found to be useful for signal assignments. Moreover, the (2'R)-[2'-2H]-labeled DNA 10-mer and 17-mer were synthesized to be used for the determination of precise structures. These stereoselective [2'-2H]-labeling made possible explicit stereospecific assignments and exact determination of the vicinal coupling constants, 3JHH, and thus the conformation of each deoxyribose ring was accurately determined.
Kojima
C
C
Institute for Protein Research, Osaka University, Japan.
Kyogoku
Y
Y
Ishido
Y
Y
Kawashima
E
E
Sekine
T
T
Hirao
I
I
Kainosho
M
M
D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009690:Nucleic Acid Conformation
D003596:Cytosine; D004247:DNA