title: Reperfusion of duodenum and pancreas following 24 hr of cold storage in Perfadex or UW solution.
abstract: We have studied the immediate reperfusion and the graft outcome of the duodenum and pancreas separately in a porcine whole-organ pancreaticoduodenal allograft transplantation model using 24 hr of cold storage. Of 19 transplantations, 12 grafts were perfused and stored in Perfadex and 7 in University of Wisconsin (UW) solution. The organ weights before and after storage were determined in 6 grafts stored in Perfadex and 6 grafts stored in UW solution in order to quantify the degree of dehydration of the graft during storage. In 6 of the grafts perfused with Perfadex, a hyperosmotic salt dextran-containing solution (HSD) was perfused into the graft aorta at reperfusion. Duodenal reperfusion and reperfusion injury were studied by measuring mucosal pH (pHi) and by microscopic examination, respectively. Pancreatic reperfusion was studied by scoring the macroscopic appearance and microscopic examination. Daily blood glucose was used to determine the endocrine function and a secretin-cholecystokinin stimulation test was performed on the second postoperative day to evaluate the exocrine function. All duodenal grafts except 1 in the UW group showed a sufficient reperfusion according to pHi and all had microscopic changes suggesting a moderate reperfusion injury. The macroscopic appearance of pancreas was significantly worse in the UW group than in the two Perfadex groups. Only 1 of 7 grafts in the UW group showed signs of endocrine function on the first postoperative day compared with 11 of 12 in the Perfadex groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, Malmö General Hospital, University of Lund, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Montgomery
authors.forename: R
authors.initials: R
authors.lastname: Källén
authors.forename: K
authors.initials: K
authors.lastname: Fält
authors.forename: U
authors.initials: U
authors.lastname: Haglund
authors.forename: A
authors.initials: A
authors.lastname: Borgström
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002951:Citrates; D015925:Cryopreservation; D004386:Duodenum / Q000637:transplantation; D005260:Female; D005978:Glutathione; D007328:Insulin; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010179:Pancreas / Q000473:pathology; D016035:Pancreas Transplantation; D011887:Raffinose; D015424:Reperfusion; D013552:Swine; D013997:Time Factors
chemical_list: D002951:Citrates; D007328:Insulin; D019188:Organ Preservation Solutions; C070005:Perfadex; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose