[Structural and immunologic studies of Proteus mirabilis 033 O-specific polysaccharide].
O-specific polysaccharide was obtained by mild acid degradation of P. mirabilis 033 lipopolysaccharide (LPS). It was found to contain N-acetyl-D-glucosamine, D-glucuronic acid and N-acetyl-L-fucosamine in a ratio 1:1:1. On the basis of the data obtained from 13C-NMR and methylation analysis, the following structure of repeating unit was established: [formula: see text] Selective removal of the D-GlcA significantly decreased reactivity of 033 O-specific polysaccharide with homologous antiserum. This component was plays an immunodominant role. Cross reactivity between anti-033 serum and disaccharide alfa-L-FucNAc-beta-D-GlcNAc containing P. vulgaris 023 and S. arizonae 059 O-specific polysaccharides was also observed.
Cedzyński
M
M
Instytut Mikrobiologii i Immunologii Uniwersytetu Lódzkiego.
Rózalski
A
A
Kotełko
K
K
Kaca
W
W
Vinogradov
E V
EV
Knirel
Y A
YA
Kochetkov
N K
NK
D000942:Antigens, Bacterial / Q000032:analysis; D003429:Cross Reactions; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011134:Polysaccharides / Q000032:analysis*; D011513:Proteus mirabilis / Q000737:chemistry* / Q000145:classification; D012703:Serotyping
D000942:Antigens, Bacterial; D011134:Polysaccharides
Badania strukturalne i immunologiczne polisacharydu O-swoistego Proteus mirabilis 033.