Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes.
Expression of listeriolysin O of Listeria monocytogenes as a function of different growth conditions was studied by performing a direct hemolysin assay, immunoblotting experiments, and an enzyme-linked immunosorbent assay. Expression of listeriolysin O was reduced at a lower growth temperatures (26 degrees C) and at higher glucose concentrations (> or = 0.3%) in the growth media. The effect of glucose appeared to be due to a change in the pH of the growth media.
Datta
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AR
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
Kothary
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MH
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001427:Bacterial Toxins; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008757:Methylglucosides / Q000494:pharmacology; D013696:Temperature
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D008757:Methylglucosides; C027020:methylglucoside; D005947:Glucose; C038705:hlyA protein, Listeria monocytogenes