Sensitive determination of zearalenone and alpha-zearalenol in barley and Job's-tears by liquid chromatography with fluorescence detection.
A sensitive and reliable method for liquid chromatographic (LC) determination of zearalenone and alpha-zearalenol in barley and Job's-tears was investigated. The method by which these toxins were determined involves addition of an internal standard (zearalenone 6'-oxime) to barley and Job's-tears samples. Extracts from grain samples were cleaned up by passage through chromatography on piperidinohydroxypropyl Sephadex LH-20 as a lipophilic gel. Individual toxins were resolved by LC on a reversed-phase (ODS) column with fluorescence detection. The detection limit is estimated to be 0.2 ng for zearalenone and alpha-zearalenol standards. Known amounts of zearalenone and alpha-zearalenol (25-1250 ng) were added to a barley sample (5 g). Average recoveries for alpha-zearalenol and zearalenone, respectively, ranged from 96 to 102% (mean CV, 3.6%) and from 96 to 103% (mean CV, 3.3%). This method is applicable to determination of alpha-zearalenol and zearalenone in barley and Job's tears with satisfactory sensitivity and accuracy.
Tanaka
T
T
National Institute of Hygienic Sciences, Kamiyoga, Tokyo, Japan. National Institute of Hygienic Sciences, Tokyo, Japan.
Teshima
R
R
Ikebuchi
H
H
Sawada
J
J
Terao
T
T
Ichinoe
M
M
D002853:Chromatography, Liquid; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D001467:Hordeum / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D015025:Zearalenone / Q000032:analysis*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis
C029659:zearalenol; D015025:Zearalenone; D015029:Zeranol