K(+)-driven sinusoidal efflux of glutathione disulfide under oxidative stress in the perfused rat liver.
Tert-butyl hydroperoxide (BHP), hydrogen peroxide and diamide caused a rapid and simultaneous release of glutathione disulfide (GSSG) and K+ in the isolated perfused rat liver. Both BHP-induced effluxes were suppressed by prior depletion of hepatic glutathione, but not by co-infusion of desferrioxamine which prevented lipid peroxidation and cell death. High K+ media decreased the GSSG efflux even though hepatic GSSG levels remained high. The GSSG and K+ effluxes were repeatable if cellular K+ recovered after a short BHP exposure. Ouabain inhibited the K+ re-uptake and decreased the response to repeated BHP challenge. Thus, sinusoidal efflux of GSSG under oxidative stress may be driven by a K+ gradient.
Masuda
Y
Y
Division of Toxicology, Niigata College of Pharmacy, Japan.
Ozaki
M
M
Aoki
S
S
D000818:Animals; D003958:Diamide / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D019803:Glutathione Disulfide; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D010084:Oxidation-Reduction; D010477:Perfusion; D010545:Peroxides / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020122:tert-Butylhydroperoxide
D010545:Peroxides; D003958:Diamide; D010042:Ouabain; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D005978:Glutathione; D011188:Potassium; D019803:Glutathione Disulfide