title: An additional locus (PI4) in the protease inhibitor (PI) gene cluster of pigs.
abstract: A further alpha-protease inhibitor system, PI4, was detected in porcine sera using either 2D agarose gel, pH 5.0-PAGE, pH 9.0, or 1D PAGE followed by immunoblotting with rabbit anti-porcine PI2 or PI3 antisera. PI4 inhibited chymotrypsin, but not trypsin. Seven allelic variants of PI4 were described. By haplotyping of alpha-protease inhibitor systems in 52 complete families it was shown that PI4 locus belongs to the PI gene cluster. The probable order of the PI loci was: PI1, PO1A, PI2, PI4, PI3.
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libĕchov.
authors.forename: D
authors.initials: D
authors.lastname: Cízová
authors.forename: E
authors.initials: E
authors.lastname: Gábrisová
authors.forename: A
authors.initials: A
authors.lastname: Stratil
authors.forename: J
authors.initials: J
authors.lastname: Hojný
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D006239:Haplotypes; D015151:Immunoblotting / Q000662:veterinary; D005810:Multigene Family; D011480:Protease Inhibitors; D013552:Swine / Q000235:genetics*
chemical_list: D001798:Blood Proteins; D011480:Protease Inhibitors