title: Morphological and virological studies in six autopsies of children with adenovirus pneumonia.
abstract: Pulmonary lesions compatible with adenovirus infection were detected by gross and microscopic examination of autopsy tissues from children aged from 5 to 34 months. Hepatic lesions indicative of systemic infection were also found in four of the children. The viral etiology was confirmed in three cases by in-situ hybridization, electron-microscopy and immunofluorescence performed in paraffin-embedded tissues, and in one case by cell culture isolation of adenovirus type 2 from nasopharyngeal exudate. Routine testing by methods additional to conventional light microscopy would probably have revealed a larger number of adenovirus infections among the 1,103 autopsy records analyzed in this study.
authors.affiliation: Departamento de Anatomia Patológica, Instituto Fernandes Figueira-FIOCRUZ, Rio de Janeiro, Brasil.
authors.forename: A G
authors.initials: AG
authors.lastname: Garcia
authors.forename: M E
authors.initials: ME
authors.lastname: Fonseca
authors.forename: M
authors.initials: M
authors.lastname: de Bonis
authors.forename: H I
authors.initials: HI
authors.lastname: Ramos
authors.forename: Z P
authors.initials: ZP
authors.lastname: Ferro
authors.forename: J P
authors.initials: JP
authors.lastname: Nascimento
mesh_terms: D000258:Adenovirus Infections, Human / Q000473:pathology*; D000260:Adenoviruses, Human / Q000648:ultrastructure; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D008099:Liver / Q000382:microbiology / Q000648:ultrastructure; D011024:Pneumonia, Viral / Q000382:microbiology / Q000473:pathology*