The use of chemiluminescence and the ELISA spot assay to identify and enumerate rare immunoglobulin switch variants.
The use of chemiluminescence and the ELISA spot assay for identifying rare immunoglobulin switch variants is described. The technique utilizes nitrocellulose membranes and allows rapid screening of a large number of cells. The number of spots can be recorded either manually or automatically by using a commercially available colony counting program. This modification of the ELISA spot assay makes it less labor intensive and time consuming and can be adapted for the search for rare cells secreting small amounts of Ig or other macromolecules.
Spira
G
G
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
Gregor
P D
PD
Scharff
M D
MD
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D014644:Genetic Variation; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D007132:Immunoglobulin Isotypes / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007134:Immunoglobulin Switch Region / Q000235:genetics; D008163:Luminescent Measurements; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M