Actions of nitric oxide-generating sodium nitroprusside in myenteric plexus of guinea pig small intestine.
Sodium nitroprusside (NaNP) was used as a donor of nitric oxide (NO) to investigate actions of NO on electrical and synaptic behavior of single myenteric neurons in guinea pig small intestine. NaNP (10 microM-1 mM) did not affect resting membrane properties of the neurons, except for an occasional decrease in input resistance and hyperpolarization attributable to suppression of excitatory transmitter release. NaNP did not alter fast nicotinic neurotransmission but suppressed noncholinergic slow excitatory postsynaptic potentials (slow EPSPs) in a concentration-dependent manner. Pretreatment with either methylene blue or oxyhemoglobin reduced the inhibitory action of NaNP on the slow EPSPs. Slow EPSP-like responses to microejected substance P or 5-hydroxytryptamine were unaffected by NaNP. The nitric oxide synthase inhibitor, N omega-nitro-L-arginine methyl ester, did not affect resting membrane excitability or excitatory synaptic events in any of the myenteric neurons. The results suggest that NO may not be released extensively as a neurotransmitter at synapses within the myenteric plexus. If myenteric neurons are exposed to NO released from nonneural sources, then the principal action is expected to be presynaptic inhibition of slow synaptic excitation.
Tamura
K
K
Department of Physiology I, School of Medicine, Tokai University, Isehara, Japan.
Schemann
M
M
Wood
J D
JD
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D005725:Ganglia, Autonomic / Q000187:drug effects / Q000502:physiology; D006168:Guinea Pigs; D007421:Intestine, Small / Q000294:innervation*; D008297:Male; D009130:Muscle, Smooth / Q000294:innervation; D009197:Myenteric Plexus / Q000187:drug effects / Q000502:physiology*; D019331:NG-Nitroarginine Methyl Ester; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D010108:Oxyhemoglobins / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects*
D010108:Oxyhemoglobins; D009599:Nitroprusside; D005576:Colforsin; D009569:Nitric Oxide; D012701:Serotonin; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester