DNA adduct detection: some applications in monitoring exposure to environmental genotoxic chemicals.
In the assessment of genotoxic risk factors in the environment, the measurement of DNA adducts in aquatic organisms and in plants may have considerable implications. Using 32P-postlabelling, we have detected DNA adducts in the liver of carp (Chondrostoma nasus) from the River Rhône (France), both downstream and upstream from a polychlorinated biphenyl incineration plant. Some of the DNA adducts were specific to downstream fish, suggesting a differential pattern of exposure. We have also detected DNA damage in needles in a declining spruce forest. We found that, in the declining forest, the amounts of DNA adducts increase in relation to the degree of damage to the needles whereas, in a healthy forest, the levels of DNA adducts were low. We have also found DNA adducts in the leaves of hops grown in fields where heptachlor residues persisted.
Pfohl-Leszkowicz
A
A
Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, Strasbourg, France.
Weber-Lotfi
F
F
Masfaraud
J F
JF
Devaux
A
A
Laouedj
A
A
Guillemaut
P
P
Malaveille
C
C
Rether
B
B
Monod
G
G
Dirheimer
G
G
D000818:Animals; D002347:Carps / Q000378:metabolism; D004247:DNA / Q000032:analysis* / Q000187:drug effects; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D008099:Liver / Q000737:chemistry; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D010944:Plants / Q000737:chemistry; D014197:Trees / Q000737:chemistry
D009153:Mutagens; D004247:DNA