Chemistry of oxidative DNA strand scission.
In order to investigate the reactivity of uracilyl-5-yl radical in fixed DNA structure, photoreaction of 5-halouracil-containing oligonucleotides was investigated. It was found that photoirradiation of BrU-containing duplex DNA provided C1' oxidation product selectively at adenine residue of ABrU sequence. In contrast, photoirradiation of IU-containing DNA gave C1' and C2' oxidation products with almost no sequence preference. Sequence specific electron transfer in duplex DNA is proposed in the photoreaction of ABrU.
Sugiyama
H
H
Division of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Japan.
Fujimoto
K
K
Hatano
K
K
Ohmori
K
K
Saito
I
I
D000225:Adenine / Q000737:chemistry; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D010084:Oxidation-Reduction; D010777:Photochemistry; D014498:Uracil / Q000737:chemistry
D003432:Cross-Linking Reagents; D014498:Uracil; D004247:DNA; D000225:Adenine