Effects of lisinopril and captopril on calcium in rat heart.
We studied the effects of lisinopril (Lis) and captopril (Cap), two angiotensin-converting enzyme inhibitors, on calcium in ischemia/reperfusion and normal rat hearts. Ischemia/reperfusion hearts were subjected to 15 min ischemia followed by 1 or 30 min reperfusion. Lis 0.1 mumol.L-1 and Cap 200 mumol.L-1 decreased the concentration of calcium in ischemia/reperfusion hearts (the content of calcium in reperfusion 1 min heart were reduced from 4.0 +/- 0.6 to 2.7 +/- 0.5 and 3.0 +/- 0.9 mumol/g dry wt respectively). In cultured cell of neonatal rat heart, both drugs inhibited the uptake of 45Ca2+. The activity of Na+,K(+)-ATPase prepared from rat heart was increased (activity increased from 15.7 +/- 2.3 in control group to 21.2 +/- 2.0 and 22.0 +/- 3.1 mumol/h mg protein in Lis and Cap groups, respectively). This calcium lowering effects of Lis and Cap may be important in protecting the ischemia/reperfusion damage of myocardium.
Wang
J F
JF
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.
Xiao
W B
WB
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002216:Captopril / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D017706:Lisinopril / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000150:complications / Q000378:metabolism / Q000517:prevention & control*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014693:Ventricular Fibrillation / Q000209:etiology / Q000517:prevention & control
D000806:Angiotensin-Converting Enzyme Inhibitors; D002216:Captopril; D017706:Lisinopril; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium