Reduction of GABA inhibition in Purkinje and cerebellar nuclei neurons in climbing fibre deafferented cerebella of rat.
GABA agonists were iontophoretically applied to Purkinje cells (PCs) of the cerebellar cortex and to neurons of the cerebellar nuclei (NCNs) in normal and in climbing fibre (CF) deafferented cerebella of rat. The experiments were performed one and three months after CF deafferentation obtained by total inferior olive destruction with 3-acetylpyridine. All control PCs were dose-dependently inhibited by GABA and muscimol and nearly all by baclofen. After CF deafferentation, the number of PCs sensitive to muscimol remained the same but the number sensitive to baclofen was greatly reduced one month later and almost absent after three months. The number of NCNs inhibited by GABA was slightly reduced one month after deafferentation compared to controls, but reduced to less than half three months after. Thus CF deafferentation of the PC leads to changes in postsynaptic sensitivity to GABA, the presumed inhibitory neurotransmitter, affecting GABAA receptors of the NCNs and GABAB receptors of the PCs.
Vigot
R
R
Laboratoire de Physiologie de la Motricité, CNRS, Université Pierre et Marie Curie, CHU Pitié-Salpêtrière, Paris, France.
Billard
J M
JM
Batini
C
C
D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D002529:Cerebellar Nuclei / Q000166:cytology / Q000502:physiology*; D003714:Denervation; D004594:Electrophysiology; D007478:Iontophoresis; D008839:Microelectrodes; D009118:Muscimol / Q000494:pharmacology; D009412:Nerve Fibers / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology*; D011689:Purkinje Cells / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects; D018080:Receptors, GABA-B / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology / Q000502:physiology*
D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D001418:Baclofen