title: Genes expressed during sexual differentiation of Chlamydomonas reinhardtii.
abstract: Four genes specifically expressed during gametogenesis of Chlamydomonas reinhardtii have been cloned and their expression patterns analyzed. mRNAs encoded by these gamete-specific genes (gas) were absent or present only at very low levels in vegetative cells and mature zygotes. In young zygotes 2 h after gamete fusion, the mRNAs of three gas genes still persisted. The gas mRNAs accumulated during gametic differentiation. The temporal patterns of accumulation of individual mRNAs differed; some started to increase early during gametogenesis, others accumulated in the late phase. The accumulation of one of the late mRNAs (gas28) was strictly light-dependent. To illustrate the utility of the genes cloned in the analysis of sexual differentiation in Chlamydomonas reinhardtii we show that in a gametogenesis-defective mutant, the expression of late genes is prevented while that of early genes is normal.
authors.affiliation: Institut für Biologie III, Albert-Ludwigs-Universität, Freiburg, Germany.
authors.forename: E D
authors.initials: ED
authors.lastname: von Gromoff
authors.forename: C F
authors.initials: CF
authors.lastname: Beck
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D005718:Gametogenesis / Q000235:genetics; D015870:Gene Expression; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger