Molecular cloning of crustacean putative molt-inhibiting hormone (MIH) precursor.
A cDNA encoding the complete precursor of the putative molt-inhibiting hormone (MIH) of the shore crab, Carcinus maenas, was isolated and sequenced. The precursor consists of a putative 35 amino acid signal peptide and the 78 amino acid mature MIH. The deduced MIH amino acid sequence is in complete agreement with the sequence previously determined by Edman degradation. In situ hybridization revealed MIH-expression in a subpopulation of large neurosecretory perikarya of the medulla terminalis X-organ in the eyestalk.
Klein
J M
JM
Institute for Zoophysiology, University of Bonn, Germany.
Mangerich
S
S
de Kleijn
D P
DP
Keller
R
R
Weidemann
W M
WM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura; D003001:Cloning, Molecular; D004247:DNA; D007447:Invertebrate Hormones / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*
D007447:Invertebrate Hormones; D011498:Protein Precursors; C012445:molt-inhibiting hormone; D004247:DNA