[Correlation of IgA class antigliadin and antiendomysial antibodies (IgA-AGA-IgA-EMA) with intestinal histology in celiac disease].
The aim of this work was to establish the diagnostic and follow up value of IgA-class antiendomysium (IgA-EmA) and IgA-class antigliadin (IgA-AGA) antibodies in celiac disease. Correlation with the intestinal histology at the different stages of the disease was evaluated, as well as its therapeutic monitoring ability. Fifty six children, twenty seven girls and twenty nine boys, aged six months to twelve years old, were studied. Thirty nine celiac children were al different diagnostic stages of the disease. Seventeen children with malabsorption symptoms and with normal intestinal histology were used as controls. Sixty blood samples were obtained simultaneously with the small intestinal biopsy. IgA-AGA (ELISA method) and IgA-EmA (immunofluorescent test performed over lower third Rhesus monkey esophagus) were determined in every blood sample. In 34 serum samples from patients with total or subtotal villous atrophy, two were negative for IgA-AGA and only one was negative for IgA-EmA. In 26 samples from patients with normal intestinal histology, two were positive for IgA-AGA and four were positive for IgA-EmA. The results for IgA-EmA had sensitivity 97%, specificity 84.6%, positive predictive value 89.2% and negative predictive value 95%. In the case of IgA-AGA were: sensitivity 94.1%, specificity 92.3%, positive predictive value 94.1%, negative predictive value 92.3%. IgA-AGA and IgA-EmA showed a high correlation with intestinal histology and are in combination powerful tools for the diagnosis and follow up of celiac patients. Besides, they provide a useful aid in the indication of a jejunal biopsy and in close monitoring of the dietary treatment compliance.(ABSTRACT TRUNCATED AT 250 WORDS)
De Rosa
S
S
Hospital de Pediatría Juan P. Garrahan, Servicio de Gastroenterología, Argentina.
Litwin
N
N
Dávila
M T
MT
Ruiz
J A
JA
Guastavino
E
E
Pini
A
A
Queralt
A M
AM
D000906:Antibodies / Q000032:analysis*; D001706:Biopsy; D002446:Celiac Disease / Q000097:blood / Q000473:pathology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007422:Intestines / Q000276:immunology* / Q000473:pathology; D008297:Male; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000906:Antibodies; D007070:Immunoglobulin A; D005903:Gliadin
Correlación de anticuerpos antigliadina y antiendomisiales clase IgA (AGA-IgA-EMA-IgA) con la histologia intestinal en la enfermedad celiaca (E C).