Isolation of a rickettsial organism (Eperythrozoon) from mice during virological testing of ixodid ticks.
A strain of Eperythrozoon (Rickettsiales: Anaplasmataceae) was isolated from suckling SPF mice (SM) inoculated with a homogenate of nymphal Ixodes ricinus (L.) ticks. The strain, passaged in the SM brain, was partially filterable through a 220-nm Millipore membrane, moderately sensitive to diethylether, and pathogenic for SM while no symptoms were observed in inoculated adult mice or male guinea pigs. A conspicuous cytopathic effect was produced in PS and SPEV (embryo pig kidney) stable cell lines. Both the cell cultures and SM could be used for the infectivity titration of the agent.
Hubálek
Z
Z
Institute of Systematic and Ecological Biology, Academy of Sciences of the Czech Republic, Brno.
Juricová
Z
Z
Halouzka
J
J
Sebek
Z
Z
D000818:Animals; D000833:Animals, Suckling; D001096:Arachnid Vectors / Q000382:microbiology*; D005260:Female; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D009174:Mycoplasma / Q000302:isolation & purification*; D013987:Ticks / Q000382:microbiology*