title: High-performance liquid chromatographic determination of concentrations of the reversible H+/K+ ATPase inhibitor SK&F 97574 in plasma.
abstract: A reversed-phase high-performance liquid chromatographic (HPLC) assay using ultraviolet spectrophotometric detection has been developed for the determination of the concentration of 3-butyryl-4-(2-methylphenylamino)-8-(2-hydroxyethoxy)quinoline (I) in rat, dog and human plasma. Prior to analysis, the protein in plasma samples was precipitated with acetonitrile containing 3-butyryl-4-(2-methylphenylamino)-8-methoxyquinoline to act as an internal standard. The supernatant layer was injected onto the HPLC column with no further clean-up. The assay requires 200 microliters of plasma and is precise and accurate within the range 25-1000 ng/ml. The mean within-run and between-run coefficients of variation were < 6% at 25 ng/ml and greater concentrations. The mean accuracy of quality control standards was generally within +/- 5% of the nominal concentration. Recovery of I and internal standard from plasma was approximately 100% over the entire assay range irrespective of species.
authors.affiliation: Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, Philadelphia, PA 17406.
authors.forename: T D
authors.initials: TD
authors.lastname: Moore
authors.forename: A C
authors.initials: AC
authors.lastname: Metcalf
authors.forename: J E
authors.initials: JE
authors.lastname: Swagzdis
authors.forename: E
authors.initials: E
authors.lastname: Doyle
mesh_terms: D000634:Aminoquinolines / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006801:Humans; D054328:Proton Pump Inhibitors; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000634:Aminoquinolines; D054328:Proton Pump Inhibitors; C084536:3-butyryl-4-(2-methylphenylamino)-8-(2-hydroxyethoxy)quinoline