title: Three-dimensional structure of recoverin, a calcium sensor in vision.
abstract: Recoverin, a recently discovered member of the EF hand superfamily, serves as a calcium sensor in vision. We report here the crystal structure of recombinant unmyristoylated recoverin at 1.9 A resolution. The four EF hands of the protein are arranged in a compact array that contrasts with the dumbbell shape of calmodulin and troponin C. A calcium ion is bound to EF hand 3, while EF hand 2 can bind samarium but not calcium in this crystal form. The other two EF hands have novel structural features that prevent or impair calcium binding. A concave hydrophobic surface formed by EF hands 1 and 2 may participate in the read out of calcium signals by recoverin and its homologs.
authors.affiliation: Beckman Laboratories for Structural Biology, Department of Cell Biology, Stanford University School of Medicine, California 94305.
authors.forename: K M
authors.initials: KM
authors.lastname: Flaherty
authors.forename: S
authors.initials: S
authors.lastname: Zozulya
authors.forename: L
authors.initials: L
authors.lastname: Stryer
authors.forename: D B
authors.initials: DB
authors.lastname: McKay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000648:ultrastructure*; D002417:Cattle; D002645:Chickens; D018360:Crystallography, X-Ray; D005136:Eye Proteins; D051597:Hippocalcin; D006801:Humans; D008074:Lipoproteins; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000648:ultrastructure; D051603:Recoverin; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; C496731:RCVRN protein, human; C496732:Rcvrn protein, mouse; D011994:Recombinant Proteins; D051603:Recoverin; D051597:Hippocalcin; D002118:Calcium