Age-related regional changes in hydroxyl radical stress and antioxidants in gerbil brain.
The levels of hydroxyl radicals and oxidized GSH have been examined as indices of oxidative stress in young (3 months), middle-aged (15 months), and old (20-24 months) gerbil brain hippocampus, cortex, and striatum. The hydroxyl radical stress was estimated by measuring the salicylate hydroxyl radical trapping products 2,5- and 2,3-dihydroxybenzoic acid. The stress was significantly higher in all three brain regions in middle-aged and old gerbils versus young animals (< or = 66.0%). Regional comparisons showed that the stress was significantly higher in cortex than in either the hippocampus or striatum of the middle-aged and old gerbils (< or = 32.0%). The ratio of oxidized to total GSH also increased progressively in middle-aged and old animals in all three brain regions (p < 0.05, < or = 41.1%), further indicating a general age-related increase in oxidative stress. Parallel to this age-related increase in oxidative stress, a significant, albeit slight (8%), decrease in neuronal number in hippocampal CA1 region was observed in both the middle-aged and old animals. Possible differences in antioxidant levels were also examined. Total GSH levels were similar across age groups (variance < 12%). However, the regional comparison showed that it was highest in striatum in all age groups. The levels of alpha-tocopherol (vitamin E) were significantly higher in the middle-aged and old animals in all three regions (< or = 70.4%). Vitamin E was highest in the hippocampus and the differences between the hippocampus and the cortex and striatum increased with age.(ABSTRACT TRUNCATED AT 250 WORDS)
Zhang
J R
JR
Upjohn Company, Kalamazoo, MI 49001.
Andrus
P K
PK
Hall
E D
ED
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D001834:Body Water / Q000378:metabolism; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism; D003342:Corpus Striatum / Q000254:growth & development / Q000378:metabolism; D005841:Gentisates; D005849:Gerbillinae; D005978:Glutathione / Q000378:metabolism; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009928:Organ Specificity; D010084:Oxidation-Reduction; D011712:Pyramidal Tracts / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism
D000975:Antioxidants; D005841:Gentisates; D062385:Hydroxybenzoates; D014810:Vitamin E; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid; D005978:Glutathione; D001205:Ascorbic Acid; C010925:2,5-dihydroxybenzoic acid