Preparation of chromosome-specific paints and complete assignment of chromosomes in the pig flow karyotype.
Sorted chromosomes from each of the 20 clusters of the male porcine bivariate flow karyotype were amplified and biotinylated using DOP-PCR. The chromosomes comprising each cluster were identified by fluorescence in situ hybridization (FISH) of the 20 probes to R-banded male pig metaphase spreads. A standard flow karyotype for the pig is presented.
Langford
C F
CF
AFRC Institute of Animal Physiology & Genetics Research, Babraham Research Station, Babraham, UK.
Telenius
H
H
Miller
N G
NG
Thomsen
P D
PD
Tucker
E M
EM
D000818:Animals; D001483:Base Sequence; D001710:Biotin; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure; D017931:DNA Primers; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005456:Fluorescent Dyes; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary*; D007621:Karyotyping / Q000662:veterinary*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000235:genetics*
D017931:DNA Primers; D005456:Fluorescent Dyes; D001710:Biotin