[Serologic diagnosis of avian salmonelloses: adjustment of an ELISA test using antigens adsorbed with the aid of anti-colibacillary sera].
A serological ELISA test for diagnosis of avian salmonellosis infections with Salmonella typhimurium or enteritidis has been established. Plates were coated half with a negative antigen and half with a positive antigen. Both negative and positive antigens were adsorbed with a monovalent agglutinant anti Escherichia coli serum prior to being distributed into wells of the plates. Sera from SPF birds and from SPF birds vaccinated and/or inoculated with numerous viruses or bacteria, or sera from conventional birds bacteriologically free of any salmonellosis were tested and the percentage of false positive reactions was inferior than 1%. In groups of birds naturally infected or experimentally inoculated with Salmonella enteritidis or typhimurium the percentages of positive individuals were ranged from 15 to more than 90% according to the doses and routes of inoculation and the delay between contamination and sampling.
Kles
V
V
Ministère de l'Agriculture et de la Forêt, Centre National d'Etudes Vétérinaires et Alimentaires (C.N.E.V.A.), Laboratoire Central de Recherches Avicole et Porcine (L.C.R.A.P.), Ploufragan, France.
Morin
M
M
Humbert
F
F
Lalande
F
F
Guittet
M
M
Bennejean
G
G
D000818:Animals; D002645:Chickens; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay; D011201:Poultry Diseases / Q000175:diagnosis*; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D012477:Salmonella enteritidis / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D013047:Specific Pathogen-Free Organisms
Diagnostic sérologique des salmonelloses aviaires: mise au point d'un test ELISA utilisant des antigènes adsorbés à l'aide de sérums anticolibacillaires.