Partial reconstruction of the lampbrush loop pair Nooses on the Y chromosome of Drosophila hydei.
We present the analysis of genomic DNA fragments that were isolated as potential segments of the lampbrush loop pair Nooses on the short arm of the Y chromosome of Drosophila hydei. More than 300 kb of DNA were recovered in BamHI lambda and cosmid clone groups. This DNA is composed of the Y-specific ay1 family of repetitive DNA sequences, and of other repetitive DNA sequences, which at least in part are also located elsewhere in the genome (Y-associated sequences). Two additional classes of DNA fragments were obtained from an EcoRI library. One of them consists of ay1 repeats without apparent interspersion, including a total of more than 300 kb of DNA. The other is composed of tandemly repeated YsI sequences, a Y-specific sequence derived from ay1. This class includes more than 400 kb of DNA, which is also not interspersed by other sequences. Our results show that only the ay1 repeats interspersed by Y-associated DNA sequences can represent parts of the 260 kb transcription unit forming the lampbrush loop, whereas the ay1 and YsI repeats without interspersion form separate and nontranscribed clusters of repetitive DNA.
Hochstenbach
R
R
Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, The Netherlands.
Pötgens
A
A
Meijer
H
H
Dijkhof
R
R
Knops
M
M
Schouren
K
K
Hennig
W
W
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D015245:Deoxyribonuclease BamHI; D004330:Drosophila / Q000235:genetics*; D015723:Gene Library; D008297:Male; D009690:Nucleic Acid Conformation; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D014998:Y Chromosome / Q000737:chemistry*
D004247:DNA; D015245:Deoxyribonuclease BamHI