title: Variations in temperature and oxygen content do not alter levels of thiobarbiturate reactive material in human breast tumour cells (ZR-75-1) incubated with gamma-linolenic acid.
abstract: The mechanism by which tumour cells may be killed in vitro by exogenous polyunsaturated fatty acids may involve lipid peroxidation. Gamma-linolenic acid caused a dose and time-dependent reduction in ZR-75-1 cell growth. However, altering either the incubator temperature (35, 37 and 39 degrees C) or the oxygen content (16, 21 and 26%) had little effect on either the growth of cells in the presence of gamma-linolenic acid or on thiobarbiturate reactive material levels over a 7 day period. Thus, small changes in cell culture conditions do not affect 18:3n-6 cytotoxicity or markers of lipid peroxidation.
authors.affiliation: EFAMOL Research Institute, Kentville, Nova Scotia, Canada.
authors.forename: R C
authors.initials: RC
authors.lastname: Cantrill
authors.forename: G W
authors.initials: GW
authors.lastname: Ells
authors.forename: K A
authors.initials: KA
authors.lastname: Chisholm
authors.forename: B J
authors.initials: BJ
authors.lastname: Hughes
authors.forename: D F
authors.initials: DF
authors.lastname: Horrobin
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D016923:Cell Death; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005453:Fluorescence; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation; D010100:Oxygen / Q000008:administration & dosage*; D013696:Temperature; D013858:Thiobarbiturates / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D008041:Linoleic Acids; D013858:Thiobarbiturates; D019787:Linoleic Acid; C029684:thiobarbituric acid; D010100:Oxygen