[Interconversion of two possible forms of endothelium-derived relaxation factor--S-nitrosocysteine and an dinitrosyl complex of iron with cysteine].
Interconversion of S-nitrosocysteine (cys-NO) and dinitrosyl iron complex with cysteine (DNIC-cys) has been observed in 15 mM Hepes-buffer (pH 7.4) which was added with cys, Fe2+ and NO. Cys-NO was mainly detected in this solution at 37 degrees C. The content of DNIC-cys was predominant in frozen solution. The destruction of DNIC-cys with 0.5 HCl or desferrioxamine resulted in the formation of cys-NO. The hypothesis was advanced that endothelium-derived relaxing factor (EDRF) is the equilibrium mixture of DNIC-cys and cys-NO. EDRF which forms in vascular endothelial cells is presented in these cells mainly as DNIC-cysteine, because endogenous cys destroys cys-NO. When EDRF appears in intercellular space with low content of cys it transforms to cys-NO.
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D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry; D003676:Deferoxamine / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry*; D009603:Nitroso Compounds / Q000737:chemistry*; D026403:S-Nitrosothiols
D009603:Nitroso Compounds; D026403:S-Nitrosothiols; D009569:Nitric Oxide; C021315:S-nitrosocysteine; D007501:Iron; D003676:Deferoxamine; D003545:Cysteine
Vzaimoprevrashchenie dvukh vozmozhnykh form éndothelial'nogo faktora relaksatsii sosudov--S-nitrosotsisteina i dinitrozil'nogo kompleksa zheleza s tsisteinom.