32P-postlabeling of DNA adducts of styrene-exposed lamination workers.
Lamination workers are exposed to high concentrations of styrene. A postlabeling method was developed for the detection of styrene-specific DNA adducts of the workers. To synthesize a standard, styrene oxide was reacted with 2'-deoxyguanosine 3'-monophosphate (dGMP) and the O6-dGMP-adduct was isolated and characterized. The human samples were assayed by the nuclease P1 version of the 32P-postlabeling technique, using magnet transfer of the adducts in chromatography. The human samples were spiked with the standards to ensure identification and quantitation. In lamination workers the O6-adducts, adjusted for adduct recovery, were detected at a level of 5 adducts/10(8) nucleotides, over five times the level in the controls.
Vodicka
P
P
Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
Vodickova
L
L
Hemminki
K
K
D002855:Chromatography, Thin Layer; D004247:DNA / Q000378:metabolism*; D003848:Deoxyguanine Nucleotides / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D016273:Occupational Exposure; D010761:Phosphorus Radioisotopes / Q000378:metabolism*; D010969:Plastics; D020058:Styrene; D013343:Styrenes / Q000378:metabolism*
D003848:Deoxyguanine Nucleotides; D010761:Phosphorus Radioisotopes; D010969:Plastics; D013343:Styrenes; D020058:Styrene; C034009:2'-deoxyguanosine 3'-phosphate; D004247:DNA