Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma.
Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe6-->Trp and Phe9-->Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intraarterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein-kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.
Conlon
J M
JM
Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178.
Olson
K R
KR
D000595:Amino Acid Sequence; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D006801:Humans; D007609:Kallidin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D007705:Kinins / Q000378:metabolism; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss; D011863:Radioimmunoassay; D017386:Sequence Homology, Amino Acid; D013552:Swine
D007705:Kinins; C084151:bradykinin, Lys-Trp(6)-Leu(9)-; D007609:Kallidin