GM3 regulates protein kinase systems in cultured brain microvascular endothelial cells.
The barrier function of endothelial cells is known to be positively regulated by protein kinase A (PKA) and negatively regulated by protein kinase C (PKC). We found that exogenously administered GM3(NeuAc) promoted PKA activity in cultured brain microvascular endothelial cells (BMECs). Other glycolipids, including GM1, sulfoglucuronyl paragloboside, and GM3(NeuGc), did not have any effect on the PKA activity of BMECs. PC12 cells did not respond to exogenously applied GM3(NeuAc). GM3(NeuAc) also suppressed the PKC activity of BMECs. Thus, GM3(NeuAc) may function as a modulator of blood-brain barrier function via the two different kinase systems.
Kanda
T
T
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0614.
Ariga
T
T
Yamawaki
M
M
Yu
R K
RK
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000201:enzymology*; D005677:G(M1) Ganglioside / Q000494:pharmacology; D005679:G(M3) Ganglioside / Q000494:pharmacology*; D007700:Kinetics; D008833:Microcirculation; D016716:PC12 Cells; D011493:Protein Kinase C / Q000378:metabolism*
D005679:G(M3) Ganglioside; D005677:G(M1) Ganglioside; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C