title: Bombesin and gastrin-releasing peptide stimulate electrogenic ion transport in cultured human endometrial epithelial cell layers.
abstract: Electrogenic ion transport across human endometrial epithelial cells grown as polarized monolayers on permeable supports was measured as an inward short-circuit current (Isc; 16.2 +/- 1.1 microA/cm2). Bombesin (10(-7) M) and human gastrin-releasing peptide (GRP; 10(-7) M) caused transient enhancement of this Isc. These effects were largely restricted to the basolateral surface of the cells; responses to apical peptide were modest in comparison with those to basolateral peptide. GRP and other bombesin-related peptides may have a role in regulation of endometrial epithelial ion transport in vivo and thereby influence the intra-uterine environment.
authors.affiliation: Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School.
authors.forename: C J
authors.initials: CJ
authors.lastname: Matthews
authors.forename: C P
authors.initials: CP
authors.lastname: Redfern
authors.forename: E J
authors.initials: EJ
authors.lastname: Thomas
authors.forename: B H
authors.initials: BH
authors.lastname: Hirst
mesh_terms: D001839:Bombesin / Q000494:pharmacology*; D001920:Bradykinin / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D004717:Endometrium / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D019886:Gastrin-Releasing Peptide; D006801:Humans; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology*; D008875:Middle Aged; D010455:Peptides / Q000494:pharmacology*
chemical_list: D010455:Peptides; D019886:Gastrin-Releasing Peptide; D001839:Bombesin; D001920:Bradykinin