Imaging by fluorescence videomicroscopy of individual single stranded DNA molecules in solution.
We demonstrate that the observation by fluorescence video-microscopy of individual single stranded DNA molecules is now possible owing to the use of an asymmetric cyanine (YOYO-1) belonging to a new class of fluorescent dyes complexing the DNA. The fluorescence of labelled DNA persists in denaturing conditions showing that the dye does not attach simply to the possible secondary structures. Furthermore it is stable under electrophoresis and enables the imaging of stretched molecules.
Auzanneau
I
I
Centre de Recherche, Paul Pascal, CNRS, Pessac, France.
Barreau
C
C
Salomé
L
L
D004277:DNA, Single-Stranded / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence / Q000379:methods*; D012996:Solutions; D013690:Television
D004277:DNA, Single-Stranded; D012996:Solutions