Time-resolved fluorometry in nucleic acid hybridization and western blotting techniques.
Time-resolved fluorometry is now used extensively for immunological assays and to a lesser extent in other research areas. In this review I describe applications of time-resolved fluorometry in nucleic acid hybridization and in blotting techniques, including Southern and Western blotting. Clearly, time-resolved fluorometry has potential for playing a major role in techniques other than immunological assays.
Diamandis
E P
EP
Department of Clinical Biochemistry, Toronto Hospital, Ontario, Canada.
D001426:Bacterial Proteins; D015153:Blotting, Western; D002614:Chelating Agents; D005063:Europium; D005470:Fluorometry; D009693:Nucleic Acid Hybridization; D019809:Streptavidin
D001426:Bacterial Proteins; D002614:Chelating Agents; D005063:Europium; D019809:Streptavidin