Production of parathyroid hormone-related protein by cultured human myeloma cells.
We examined whether or not parathyroid hormone-related protein (PTH-rP) is produced in cultured human myeloma cells. PTH-rP protein was detected in the cell extract of one (U266) of 6 myeloma cell lines examined by radioimmunoassay. Also we demonstrated the expression of PTH-rP mRNA in all of the 6 lines. These findings indicate that PTH-rP may be one of the factors responsible for the bone destruction or hypercalcemia in multiple myeloma.
Suzuki
A
A
Second Department of Internal Medicine, Faculty of Medicine, Kyoto University, Kyoto, Japan.
Takahashi
T
T
Okuno
Y
Y
Tsuyuoka
R
R
Sasaki
Y
Y
Fukumoto
M
M
Katakami
H
H
Kita
K
K
Shibuya
T
T
D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000378:metabolism*; D044162:Parathyroid Hormone-Related Protein; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D012333:RNA, Messenger