Isolation and analysis of the rat genomic sequence encoding Cu/Zn superoxide dismutase.
A rat genomic DNA (SOD1) encoding Cu/Zn superoxide dismutase (SOD1) (superoxide; superoxide oxidoreductase, EC 1.15.1.1) was cloned and sequenced. The rat SOD1 gene consisted of five exons and four introns spanning about 6 kb. The transcription start point (tsp) was observed 93 bp upstream from the ATG codon by primer extension analysis. The 5'-flanking sequence of SOD1 contained two CCAAT box motifs, a TATA box and four GC-like boxes. In the 3'-flanking region of SOD1, a polyadenylation signal, consensus sequence YGTGTTYY, and a G/T cluster were observed. A rat identifier (ID) sequence, a repetitive element of the rat genome, was located at between 569 and 484 bp upstream from the tsp.
Kim
Y H
YH
Department of Molecular Biology, Seoul National University, South Korea.
Yoo
H Y
HY
Jung
G
G
Kim
J Y
JY
Rho
H M
HM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D016384:Consensus Sequence; D004247:DNA; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011061:Poly A; D051381:Rats; D015183:Restriction Mapping; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D003062:Codon; D011061:Poly A; D004247:DNA; D013482:Superoxide Dismutase