Expression of the proliferation-associated Ki-67 antigen in bovine testicular tubular cells during the seminiferous epithelial cycle, demonstrated with the MIB-1 antibody.
Ki-67 expression in the seminiferous tubule of the bovine testis was studied by immunohistochemistry during the seminiferous epithelial cycle using the monoclonal antibody MIB-1. Spermatogonial proliferation is most obvious in stages 5-7, and 8, when B-spermatogonia divide. A lower rate of spermatogonial propagation is observed preceding or during meiosis in stages 1-4. The MIB-1 antibody also gives positive results with some post-spermatogonial tubular cells. Preleptotenes passing through S-phase in stage 1 reveal positive nuclei. During prophase of meiosis I pachytenes react strongly, diplotenes react in an attenuated manner, while leptotenes and zygotenes stay negative. Secondary spermatocytes seen in stage 4 are positive as are the chromosomes during meta- and anaphase of the meiotic divisions. Post-meiotic spermatids are also decorated but stop Ki-67 expression abruptly at the end of stage 4. Sertoli cells are negative.
Wrobel
K H
KH
Institut für Anatomie, Universität Regensburg, Germany.
Kujat
R
R
Lutz
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000378:metabolism; D002417:Cattle; D002455:Cell Division; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D008297:Male; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism*; D012670:Seminiferous Epithelium / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D012671:Seminiferous Tubules / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013091:Spermatogenesis / Q000276:immunology / Q000502:physiology
D000911:Antibodies, Monoclonal; D000941:Antigens; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins