Purification and characterization of RNases from fission yeast under nitrogen-starvation.
Two RNases were purified from nitrogen-starved fission yeast cells in which cellular RNA was being degraded drastically. The two RNases showed similar properties. Their molecular weight in native form was about 170kDa. They were endoRNases which required divalent cations.
Uritani
M
M
Department of Chemistry, Faculty of Science, Shizuoka University, Japan.
Iwasawa
J
J
D002413:Cations, Divalent; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009584:Nitrogen / Q000378:metabolism*; D012260:Ribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000378:metabolism
D002413:Cations, Divalent; D012260:Ribonucleases; D009584:Nitrogen