Axenic cultivation of a pathogenic Phytomonas species isolated from tomato fruit, and from its phytophagic insect vector, Phthia picta (Hemiptera: Coreidae).
Axenic cultures of Phytomonas sp. were obtained from naturally infected tomatoes and from Phthia picta, a predator of tomato plants, by using a biphasic medium with Roitman's complex medium overlaying rabbit blood-agar slants. Light and electron microscopy of both isolates showed a similarity of morphological characteristics among the flagellates in fresh material or after cultivation. Other properties, including their agglutinability with the haemolymph of Phthia picta, suggest that these isolates are virtually identical.
Fiorini
J E
JE
Escola de Farmácia e Odontologia de Alfenas, Brasil.
de Faria e Silva
P M
PM
Brazil
R P
RP
Attias
M
M
Esteves
M J
MJ
Angluster
J
J
D000371:Agglutination; D000818:Animals; D005856:Germ-Free Life / Q000502:physiology*; D006430:Hemiptera / Q000469:parasitology*; D006458:Hemolymph / Q000378:metabolism; D007303:Insect Vectors / Q000469:parasitology*; D008854:Microscopy, Electron; D010935:Plant Diseases / Q000469:parasitology; D011528:Protozoan Infections / Q000473:pathology / Q000503:physiopathology; D014351:Trypanosomatina / Q000302:isolation & purification* / Q000502:physiology* / Q000648:ultrastructure; D014675:Vegetables / Q000469:parasitology*