title: Effect of injury on mast cells of rat gastrocnemius muscle with respect to gravitational exposure.
abstract: The proteolytic enzyme, chymase, was used to identify mast cells in rat gastrocnemius muscles which were crush-injured or incised in order to determine if mast cells exhibited proliferation and degranulation. Some of the crush-injured rats were subjected to 0 g for 14 days after injury on the Cosmos 2044 satellite to study the effects of weightlessness on the mast cell response. A variety of ground-based injured models were used, including a group of hindlimb-unloaded animals acting as controls and testing the suitability of the hindlimb-unloaded animal as a model for muscle healing during weightlessness. In most cases, the numbers of mast cells and their apparent size increased after injury. When mast cell degranulation was evident, the granules containing chymase often were free in the loose connective tissue and along the edge of myofibers. The mast cell response was most exaggerated in animals subjected to 0 g and least visible in the hindlimb-unloaded ones. Thus, gravitational stress may influence mast cell physiology and the hindlimb-unloaded animal may not be a good model for investigating muscle healing.
authors.affiliation: Department of Physiology, West Virginia University, Morgantown 26506.
authors.forename: W T
authors.initials: WT
authors.lastname: Stauber
authors.forename: V K
authors.initials: VK
authors.lastname: Fritz
authors.forename: T E
authors.initials: TE
authors.lastname: Burkovskaya
authors.forename: E I
authors.initials: EI
authors.lastname: Ilyina-Kakueva
mesh_terms: D000818:Animals; D002455:Cell Division; D053818:Chymases; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006112:Gravitation; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000473:pathology* / Q000502:physiology; D008954:Models, Biological; D009132:Muscles / Q000201:enzymology / Q000473:pathology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012426:Russia; D012697:Serine Endopeptidases / Q000032:analysis; D013026:Space Flight; D014893:Weightlessness
chemical_list: D012697:Serine Endopeptidases; D053818:Chymases