Kinetics of E2A basic helix-loop-helix-protein expression during myelopoiesis and primary B cell differentiation.
The DNA-binding factors encoded by the E2A gene, which include the basic helix-loop-helix proteins E12 and E47, are thought to regulate Ig gene expression. In the rat these factors are products of the Pan locus and are referred to as Pan-2 and Pan-1, respectively. To determine when during B cell differentiation Pan (E2A) DNA-binding proteins are first expressed, cells from a newly developed rat long term lymphoid bone marrow culture system were analyzed molecularly and phenotypically using a newly defined anti-Pan mAb. The data indicate that Pan-expressing cells appear in B cell progenitors before the appearance of the CD45R B lineage-associated Ag or microH chain protein. The results also indicate that Pan proteins are present in cells that had undergone D-JH rearrangements. Finally, although Pan (E2A) proteins are present in myeloid precursors, expression is extinguished as differentiation into mature myeloid cells occurs.
Xin
X Q
XQ
Department of Biochemistry, University of California, Riverside 92521-0121.
Nelson
C
C
Collins
L
L
Dorshkind
K
K
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology*; D001483:Base Sequence; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D046508:Culture Techniques; D004268:DNA-Binding Proteins / Q000032:analysis*; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D018257:Helix-Loop-Helix Motifs; D006410:Hematopoiesis; D007700:Kinetics; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D051820:TCF Transcription Factors; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors / Q000032:analysis*
D004268:DNA-Binding Proteins; D051820:TCF Transcription Factors; C544913:Tcf7l1 protein, mouse; C553060:Tcf7l1 protein, rat; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors; D017493:Leukocyte Common Antigens