Interleukin-10 inhibits endotoxin-induced tissue factor mRNA production by human monocytes.
In Gram-negative septic shock, human monocytes synthesize and express on their cytoplasmic membrane tissue factor (TF), a potent activator of the coagulation cascades. The role of TF in triggering disseminated intravascular coagulation (DIC) in these patients appears to be clear. We report the suppressive effect of interleukin-10 (IL-10) on endotoxin-induced TF activity and antigen levels, and on the expression of TF mRNA levels in human monocytes. These results emphasize the potential therapeutic value of this cytokine in septic shock, a condition still associated with a high mortality rate.
Ramani
M
M
INSERM U294, CHU Xavier Bichat, Paris, France.
Ollivier
V
V
Khechai
F
F
Vu
T
T
Ternisien
C
C
Bridey
F
F
de Prost
D
D
D006801:Humans; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000502:physiology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D013925:Thromboplastin / Q000096:biosynthesis / Q000235:genetics*
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D016753:Interleukin-10; D013925:Thromboplastin