title: Reversed-phase high-performance liquid chromatography determination of quinine in plasma, whole blood, urine, and samples dried on filter paper.
abstract: The analysis of quinine in whole blood, plasma, urine, and samples dried on filter paper is described. Extraction was made with toluene followed by back-extraction into phosphate buffer. A reversed-phase liquid chromatography system with fluorescence detection was used. The within-day coefficient of variation of the method was 4-10% at the lower limit of determination (2 nM in plasma and 50 nM in whole blood, dried samples, and urine) and 2-4% at 10 microM. The quinine concentration was found to be lower in whole blood than in plasma (mean ratio, plasma-whole blood, 1.17). The concentration in capillary blood was lower than that in venous blood (mean ratio, capillary blood-venous blood, 0.93).
authors.affiliation: Department of Clinical Pharmacology, Huddinge University Hospital, Sweden.
authors.forename: O
authors.initials: O
authors.lastname: Ericsson
authors.forename: M
authors.initials: M
authors.lastname: Fridén
authors.forename: U
authors.initials: U
authors.lastname: Hellgren
authors.forename: L L
authors.initials: LL
authors.lastname: Gustafsson
mesh_terms: D002196:Capillaries / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005374:Filtration; D006801:Humans; D010209:Paper; D011803:Quinine / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014680:Veins / Q000378:metabolism
chemical_list: D011803:Quinine