title: Structure of heparan sulfate from the fresh water mollusc Anomantidae sp: sequencing of its disaccharide units.
abstract: 1. The disaccharide sequences of a heparan sulfate isolated from Anomantidae sp. was determined with the aid of heparitinase I, heparitinase II from Flavobacterium heparinum, mollusc beta-glucuronidase and alpha-N-acetylglucosaminidase besides nitrous acid degradation and chemical analyses. 2. Like the mammalian heparan sulfates the mollusc heparan sulfate is composed of different oligosaccharide blocks of N-acetylated disaccharides, N-sulfated disaccharides and N,6-sulfated disaccharides and has in its nonreducing end the monosaccharide glucosamine 2,6-disulfate. 3. The oligosaccharides produced by heparitinase I degradation contain at their reducing ends a N-acetylated, 6-sulfated disaccharide. 4. These and other results lead to the conclusion that the general structure of the heparan sulfate is maintained through evolution.
authors.affiliation: Departamento de Bioquimica, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
authors.forename: T M
authors.initials: TM
authors.lastname: Ferreira
authors.forename: M G
authors.initials: MG
authors.lastname: Medeiros
authors.forename: C P
authors.initials: CP
authors.lastname: Dietrich
authors.forename: H B
authors.initials: HB
authors.lastname: Nader
mesh_terms: D000107:Acetylation; D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D005591:Chemical Fractionation; D004187:Disaccharides / Q000737:chemistry*; D006497:Heparan Sulfate / Q000737:chemistry*; D008969:Molecular Sequence Data; D008974:Mollusca / Q000737:chemistry*; D011133:Polysaccharide-Lyases / Q000032:analysis; D017385:Sequence Homology; D013045:Species Specificity; D013431:Sulfates
chemical_list: D004187:Disaccharides; D006497:Heparan Sulfate; D011133:Polysaccharide-Lyases; D013431:Sulfates; C083872:heparatinase I; C083873:heparatinase II