Mutations in the protein phosphatase 1 gene at 87B can differentially affect suppression of position-effect variegation and mitosis in Drosophila melanogaster.
The suppressor of position effect variegation (PEV) locus Su-var(3)6 maps to 87B5-10. The breakpoints of deficiencies that define this interval have been placed on a 250-kb molecular map of the region. The locus is allelic to the ck19 complementation group previously shown to encode a type 1 serine-threonine protein phosphatase (PP1) catalytic subunit. When introduced into flies by P element-mediated transformation, a 5.8-kb genomic fragment carrying this gene overcomes the suppressor phenotype of Su-var(3)6(01) and recessive lethality of all mutations of the locus. Four of the mutant alleles at the locus show a broad correlation between high levels of suppression of PEV, a high frequency of aberrant mitosis and low PP1 activity in larval extracts. However, some alleles with low PP1 activity show weak suppression of PEV with a high frequency of abnormal mitosis, whereas others show strong suppression of PEV with normal mitosis. The basis for these discussed.
Baksa
K
K
Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
Morawietz
H
H
Dombrádi
V
V
Axton
M
M
Taubert
H
H
Szabó
G
G
Török
I
I
Udvardy
A
A
Gyurkovics
H
H
Szöör
B
B
D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005816:Genetic Complementation Test; D006579:Heterozygote; D008297:Male; D008938:Mitosis / Q000235:genetics*; D009030:Mosaicism; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D054645:Protein Phosphatase 1; D012097:Repressor Proteins / Q000235:genetics; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D012097:Repressor Proteins; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1