Analysis of BCR/ABL abnormalities in mRNA from 20-year-old paraffin-embedded tissue for BCR/ABL rearrangement by polymerase chain reaction.
We studied whether it is possible to obtain sufficient mRNA from old paraffin-embedded samples of spleen and bone marrow for reverse transcription and polymerase chain reaction analysis. Spleen tissue from 6 subjects with chronic myelogenous leukemia was studied for rearrangement of BCR and ABL genes. Analysis was successful in 4 cases. These data indicate the feasibility of retrospective analysis of tissue samples of special interest by molecular techniques.
Aurer
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Department of Medicine, UCLA School of Medicine 90024-1678.
Juhbashi
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Sekine
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Tomonaga
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Gale
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RP
D000328:Adult; D000368:Aged; D001853:Bone Marrow; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics* / Q000528:radiation effects; D016313:Genes, abl / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009689:Nuclear Warfare; D010232:Paraffin; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics* / Q000528:radiation effects; D013154:Spleen; D013997:Time Factors; D014021:Tissue Preservation; D014158:Transcription, Genetic
D012333:RNA, Messenger; D010232:Paraffin