Enhanced lymphatic delivery of monoclonal antibody following OK432 pretreatment.
The effect of OK432 on the lymphatic delivery of monoclonal antibody (Mab) was investigated by injecting [125I]-Mab A7 into BALB/c mice pre-treated with OK432, and measuring the radioactivity in the regional lymph nodes. The [125I]-Mab A7, when administered subcutaneously to the foot pad, preferentially accumulated in the ipsilateral popliteal and para-aortic lymph nodes. Treatment with OK432 prior to antibody injection significantly enhanced the accumulation of Mab A7 in these regional lymph nodes. These findings suggest that pretreatment with OK432 may constitute a promising method of enhancing the lymphatic delivery of Mab, and improving the efficacy of therapy directed against lymphatic malignancies.
Kitamura
K
K
First Department of Surgery, Kyoto Prefectural University of Medicine.
Takahashi
T
T
Yamaguchi
T
T
Noguchi
A
A
Otsuji
E
E
Tsurumi
H
H
Yamaoka
N
N
Miyagaki
T
T
Kotani
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D007457:Iodine Radioisotopes; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism; D008207:Lymphatic Metastasis; D008208:Lymphatic System / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010844:Picibanil / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D010844:Picibanil