Transient HTLV-I infection of a human glioma cell line following cell-free exposure.
The human T-lymphotropic virus type I (HTLV-I) has been recently associated with cases of tropical spastic paraparesis and human myelopathy. In order to study whether cells of neuroectodermic origin were susceptible to HTLV-I infection, a human glioma cell line T67 was exposed in vitro to HTLV-I by a cell-free method of virus transmission. The presence of HTLV-I proviral DNA was analyzed by polymerase chain reaction 3, 7, and 14 days after infection. The results showed the presence of LTR, pol, and tax sequences within glioma cell line 3 days after the infection. However after 7 and 14 days, detection of HTLV-I sequences remarkably decreased. P19 expression peaked 7 days after infection and decreased in the following week. These data provide evidence that cell-free transmission of HTLV-I results in transient infection of cells of glial origin.
Graziani
G
G
Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Rome, Italy.
Faraoni
I
I
Zhang
J
J
Caronti
B
B
Lauro
G
G
Bonmassar
E
E
Macchi
B
B
D004279:DNA, Viral / Q000032:analysis; D015683:Gene Products, gag / Q000096:biosynthesis; D015486:HTLV-I Antigens / Q000096:biosynthesis; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D009000:Monocytes / Q000382:microbiology; D009457:Neuroglia / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D054301:gag Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015683:Gene Products, gag; D015486:HTLV-I Antigens; D015682:Retroviridae Proteins, Oncogenic; D054301:gag Gene Products, Human Immunodeficiency Virus; C060955:p19 protein, Human T-lymphotropic virus 1