Molecular cloning and characterization of actin genes from Echinococcus granulosus.
An Echinococcus granulosus genomic library has been screened with a mouse beta-actin cDNA probe. Two clones carrying DNA fragments of about 15 kb, possibly derived from the same genome region, have been isolated. This 15-kb genomic region includes 2 actin-related sequences (EgactI and EgactII) separated by about 4 kb. The nucleotide sequences of both genes were determined. The EgactI sequence presents no introns, but an intron of 591 bp was observed in the EgactII sequence. The genes potentially encode 375 and 376 amino-acid-long actins, respectively, with a homology of 85.3%. The deduced amino acid sequences from both genes were compared to the actin sequences from other organisms, showing similarities ranging from 63.5% to 90.6%. The nucleotide sequence of a partial actin cDNA clone has been determined. The deduced amino acids sequence showed a homology of 90.3% and 88.0% in relation to the EgactI and EgactII sequences respectively, suggesting the existence of at least one more actin gene in E. granulosus. This hypothesis is reinforced by the number of bands detected in the Southern blot analysis. Experiments based on the amplification of DNA segments using 3'-specific actin primers indicate that the EgactI gene is transcribed in protoscoleces.
da Silva
C M
CM
Departamento de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Henrique
Ferreira
B
B
Picón
M
M
Gorfinkiel
N
N
Ehrlich
R
R
Zaha
A
A
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004446:Echinococcus / Q000235:genetics*; D005784:Gene Amplification; D017238:Genes, Helminth; D015698:Genomic Library; D008969:Molecular Sequence Data
D000199:Actins; D018076:DNA, Complementary