[Rat bladder ubiquitin-like molecule: isolation, purification and N-terminal sequencing].
A Ubiquitin-like peptide was accidentally isolated from rat bladder by using 5% acetic acid wash while we were isolating antibacterial peptides. The purified molecule was obtained by reverse phase high performance liquid chromatography. Gas phase microsequence analysis indicated the N-terminal sequences of the molecule as follows: MET-GLN-ILE-PHE-VAL-LYS-THR-LEU-THR-GLY-LYS-THR-ILE-THR-LEU- GLU-VAL-GLU-PRO-SER-ASP-THR-ILE-GLU-ASN, which is homologous to human ubiquitin. Ubiquitin plays a role in the differentiation of pre-B lymphocytes, Thus, it is suggested from the findings of this molecule and the endogenous antibacterial polypeptides in mucosa or mucosal epithelium that mucosal epithelium also might be one of immune cells or immunity-associated cells, which may secrete effector molecules directly to kill adherent microbes and produce regulating factors in mucosal immune response.
Wang
B
B
School of Basic Medical Sciences, UCLA-Center for the Health Sciences.
Harwig
S S
SS
Lehrer
R I
RI
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D014452:Ubiquitins / Q000737:chemistry / Q000302:isolation & purification*; D001743:Urinary Bladder / Q000737:chemistry* / Q000276:immunology
D014452:Ubiquitins