Altered hepatic production of apolipoproteins B and E in the fasted septic rat: factors in the development of hypertriglyceridemia.
The etiology of hypertriglyceridemia associated with sepsis remains unclear, but we will attempt to elucidate its character by studying the hepatic production of apolipoproteins B and E. Male Lewis rats (260-330 g) were assigned to two groups, control (n = 5) and septic (n = 5). The septic group was injected with 2 x 10(8) live Escherichia coli colonies/100 g body wt. Food was removed from all rats after injections. Twenty-four hours later a recirculating in situ liver perfusion was performed for 120 min with KRB buffer, containing L-[35S]methionine. The production of apolipoprotein B (apo B), apolipoprotein E (apo E), albumin, and transferrin was determined by immunoprecipitation. The septic rats showed a protein-specific response to sepsis. The total protein secreted increased throughout each perfusion, septic greater than control. Apo B production was increased 2.6-fold in the septic versus control groups (P = 0.037), while apo E production was decreased by 2.9 times control (P = 0.036). Albumin production was decreased 2-fold in the septic group (P = 0.002). The increased hepatic production of apo B represents an increased number of very low density lipoprotein (VLDL) particles and contributes to the elevated VLDL triglyceride levels seen in sepsis. In contrast, decreased apo E production may result in a diminished ability for peripheral and/or hepatic receptor recognition of VLDL and VLDL remnants, respectively. Each of these changes are factors in the development of hypertriglyceridemia in sepsis.
Tripp
R J
RJ
Department of Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Tabares
A
A
Wang
H
H
Lanza-Jacoby
S
S
D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D001055:Apolipoproteins B / Q000096:biosynthesis*; D001057:Apolipoproteins E / Q000096:biosynthesis*; D016470:Bacteremia / Q000150:complications* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000150:complications* / Q000378:metabolism; D005215:Fasting; D015228:Hypertriglyceridemia / Q000209:etiology*; D007163:Immunosorbent Techniques; D008079:Lipoproteins, VLDL / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D014176:Protein Biosynthesis; D051381:Rats; D011917:Rats, Inbred Lew; D014168:Transferrin / Q000096:biosynthesis; D014280:Triglycerides / Q000097:blood
D000418:Albumins; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D008079:Lipoproteins, VLDL; D014168:Transferrin; D014280:Triglycerides; C030106:very low density lipoprotein triglyceride