Schistosoma mansoni: immunoreactivity of human sera with the surface antigen Sm23.
Sm23, a surface antigen of Schistosoma mansoni, elicits an immune response in patients infected with the parasite, although to a lesser degree than Sj23, the homologue of Sm23 in Schistosoma japonicum. To characterize the host immune response to Sm23, we have expressed full-length Sm23 using the baculovirus expression system and have also expressed the N-terminal 133 amino acids and the 85 C-terminal amino acids by means of the prokaryotic vector pGEX. A total of 70 sera from patients from Sudan and Egypt were examined for alpha-Sm23 antibodies in an ELISA and in Western blot analysis using both the C-terminal polypeptide and the full-length protein. A subset of these sera was also tested for reactivity with the N-terminal polypeptide. The alpha-Sm23 antibody titers in infected patients varied widely and were not correlated with egg counts or age of the individuals. Most of the seroreactivity was directed against the C-terminal polypeptide.
Köster
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B
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Hall
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MR
Strand
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D000367:Age Factors; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015801:Helminth Proteins; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D011223:Praziquantel / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000188:drug therapy / Q000276:immunology*; D014162:Transfection
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D017931:DNA Primers; D015801:Helminth Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C072633:Sj23 antigen, Schistosoma japonicum; D011223:Praziquantel