Construction of the Bacillus subtilis chromosome physical map and the strategy for mapping newly isolated genes in one membrane filter for hybridization.
A complete physical map of the Bacillus subtilis 168 chromosome was constructed. The merging of this physical map is expected not only to provide important insights into the organization and rearrangement of genes of this species but also to be a powerful means for the genome analysis. One of the most practical aspects is rapid and accurate mapping of newly isolated genes using a single membrane filter for hybridization. This protocol proved that not only unique genes but also multiple homologous genes dispersed on the chromosome can be physically mapped.
Itaya
M
M
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
D001412:Bacillus subtilis / Q000235:genetics*; D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods; D002876:Chromosomes, Bacterial; D005798:Genes, Bacterial; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization / Q000379:methods; D009876:Operon; D012335:RNA, Ribosomal / Q000235:genetics; D012407:rRNA Operon / Q000235:genetics
D008567:Membranes, Artificial; D012335:RNA, Ribosomal