Collagen shields impregnated with gentamicin-dexamethasone as a potential drug delivery device.
We investigated the ability of collagen shields impregnated with gentamicin sulfate and dexamethasone to deliver medication into rabbit eyes. We compared the aqueous humor gentamicin and dexamethasone levels delivered by collagen shield and subconjunctival injection therapy at five time points over a ten-hour period, by using a fluorescence polarization immunoassay and high-pressure liquid chromatography. Our in vitro studies showed that impregnated collagen shields released most of the gentamicin within the first 30 minutes of elution, whereas dexamethasone was released gradually over a ten-hour period. The collagen shields impregnated with gentamicin and dexamethasone produced aqueous gentamicin levels that were significantly lower (P = .014) than those produced by subconjunctival injection therapy at 30 minutes and that were comparable to subconjunctival injection therapy at the other time points. Minimum inhibitory concentrations of gentamicin (approximately 4 micrograms) were observed in aqueous humor within 30 minutes in rabbits that received subconjunctival injections and at one hour in rabbits treated with impregnated collagen shields. The impregnated collagen shields produced aqueous dexamethasone levels that were significantly lower (P = .004) than those produced by subconjunctival injection therapy at one hour, significantly higher (P = .028) than subconjunctival injection therapy at six hours, and comparable to subconjunctival injection therapy at the other time points. Collagen shield delivery of gentamicin-dexamethasone may be comparable to subconjunctival injections and provide an alternative therapy after intraocular surgery.
Milani
J K
JK
Jules Stein Eye Institute, UCLA Center for Health Sciences 90024-7000.
Verbukh
I
I
Pleyer
U
U
Sumner
H
H
Adamu
S A
SA
Halabi
H P
HP
Chou
H J
HJ
Lee
D A
DA
Mondino
B J
BJ
D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003094:Collagen; D003228:Conjunctiva; D003315:Cornea / Q000187:drug effects / Q000378:metabolism; D003907:Dexamethasone / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004337:Drug Carriers; D004338:Drug Combinations; D016503:Drug Delivery Systems; D005260:Female; D016231:Fluorescence Polarization Immunoassay; D005839:Gentamicins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D007267:Injections; D011817:Rabbits
D004337:Drug Carriers; D004338:Drug Combinations; D005839:Gentamicins; D003907:Dexamethasone; D003094:Collagen