Two simple procedures for releasing chromatin from routinely fixed cells for fluorescence in situ hybridization.
We describe two methods for releasing chromatin from routinely harvested and fixed cells. Using fluorescence in situ hybridization with combinations of probes from the HLA class II region, we show that good signals can be obtained on free chromatin fibers enabling determination of relationships between closely adjacent or overlapping probes.
Fidlerová
H
H
Human Cytogenetics and Human Immunogenetics Laboratories, Imperial Cancer Research Fund, London, UK.
Senger
G
G
Kost
M
M
Sanseau
P
P
Sheer
D
D
D002843:Chromatin / Q000737:chemistry*; D002874:Chromosome Mapping / Q000379:methods; D002896:Chromosomes, Human, Pair 6; D015342:DNA Probes; D005404:Fixatives; D005559:Formamides / Q000737:chemistry; D005802:Genes, MHC Class II; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008285:Major Histocompatibility Complex; D012972:Sodium Hydroxide / Q000737:chemistry
D002843:Chromatin; D015342:DNA Probes; D005404:Fixatives; D005559:Formamides; C031066:formamide; D012972:Sodium Hydroxide