On the synthesis and characterization of N-formyl glutathione and N-acetyl glutathione.
Treatment of glutathione (GSH) with formic acid and acetic anhydride leads to preparation of crystalline N-formyl GSH. This is the first characterized preparation of N-formyl GSH; this product was previously erroneously thought to be N-acetyl GSH. Authentic N-acetyl GSH (crystalline) was prepared by treating GSH with acetic acid and acetic anhydride. The N-acylated derivatives were characterized by 1H NMR and mass spectroscopy.
Levy
E J
EJ
Department of Biochemistry, Cornell University Medical College, New York, New York 10021.
Anderson
M E
ME
Meister
A
A
D000089:Acetic Anhydrides; D005561:Formates; D005978:Glutathione / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods
D000089:Acetic Anhydrides; D005561:Formates; D007202:Indicators and Reagents; C030544:formic acid; C084569:N-acetylglutathione; C031800:acetic anhydride; C084568:N-formylglutathione; D005978:Glutathione