Construction of an ecotropic retroviral vector expressing human insulin-like growth factor-I.
There is increasing evidence that IGF-I plays an autocrine role in a wide range of human tumours, including, in particular, adenomas of the thyroid epithelium. To investigate this further, we set out to generate a retrovirus vector which would permit experimental manipulation of the expression of IGF-I in normal and neoplastic epithelial cells. We describe here the construction and validation of a high-titre ecotropic vector which transduces stable expression and secretion of human IGF-IA, as shown by analysis of mRNA and conditioned medium from rodent epithelial target cells. This vector should be a useful tool for assessing the contribution of abnormal IGF-I expression to the neoplastic phenotype.
McClure
F
F
Department of Pathology, University of Wales College of Medicine, Cardiff, UK.
Dawson
T
T
Wynford-Thomas
D
D
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017077:Culture Media, Conditioned; D017931:DNA Primers; D005822:Genetic Vectors; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011863:Radioimmunoassay; D015183:Restriction Mapping; D012190:Retroviridae; D014162:Transfection
D017077:Culture Media, Conditioned; D017931:DNA Primers; D007334:Insulin-Like Growth Factor I