title: Dauricine and anisodamine inhibited leukotrienes- and platelet activating factor-induced DNA synthesis and proliferation of bovine cerebral microvascular smooth muscle cells in culture.
abstract: The effects of leukotrienes (LT) and platelet activating factor (PAF) on DNA synthesis and proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC) were studied. At 100 pmol.L-1, LTB4, LTC4, LTD4, and PAF promoted the DNA synthesis by 44%, 50%, 48%, and 57%, and enhanced the cell proliferation by 33%, 47%, 27%, and 40%, respectively. Dauricine and anisodamine inhibited the DNA synthesis of the cells induced by LT and PAF (0.1-100 mumol.L-1). These results indicate the bright future of the 2 drugs in the prevention and treatment of cerebral vascular diseases.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zeng
authors.forename: D W
authors.initials: DW
authors.lastname: Ju
authors.forename: D X
authors.initials: DX
authors.lastname: Sun
authors.forename: Y C
authors.initials: YC
authors.lastname: Rui
mesh_terms: D000470:Alkaloids; D000818:Animals; D044182:Benzylisoquinolines; D001921:Brain / Q000098:blood supply; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D007546:Isoquinolines / Q000494:pharmacology*; D020024:Leukotriene Antagonists; D008833:Microcirculation; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D012991:Solanaceous Alkaloids / Q000494:pharmacology*; D044005:Tetrahydroisoquinolines
chemical_list: D000470:Alkaloids; D044182:Benzylisoquinolines; D007546:Isoquinolines; D020024:Leukotriene Antagonists; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D012991:Solanaceous Alkaloids; D044005:Tetrahydroisoquinolines; C003922:anisodamine; C035934:dauricine; D004247:DNA