title: Two different types of ADP-ribosyltransferase C3 from Clostridium botulinum type D lysogenized organisms.
abstract: We examined production of ADP-ribosyltransferase C3 in 11 strains of Clostridium botulinum type C and D and their nontoxigenic derivatives. Antisera to C3 proteins of type C organisms divided C3 proteins roughly into at least two groups, bearing no relation to their bacterial types. The C3 gene of type D strain South African was isolated from a toxigenic phage library, and the complete sequence of the C3 gene was determined. The C3 protein of type D strain South African had 98% homology to the C3 protein of type C strain 003-9 and 66% homology to that of type D strain 1873. These results indicate that there are two types of C3 protein in type D organisms, as there are in type C organisms.
authors.affiliation: Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Moriishi
authors.forename: B
authors.initials: B
authors.lastname: Syuto
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Oguma
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: N
authors.initials: N
authors.lastname: Abe
authors.forename: M
authors.initials: M
authors.lastname: Naiki
mesh_terms: D036002:ADP Ribose Transferases / Q000145:classification / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D001483:Base Sequence; D001905:Botulinum Toxins; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008242:Lysogeny; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins