Stimulation of hematopoietic cell recovery by tetrachlorodecaoxide in sublethally irradiated mice.
This study investigated whether tetrachlorodecaoxide (TCDO), a potent wound-healing agent, promotes recovery of hematopoietic tissue of mice depleted by sublethal X irradiation. Mice were exposed to 3 Gy total-body irradiation and were given 1 ml/kg TCDO intravenously daily from day 1 to 5 or day 4 to 8 after irradiation. The effect was assessed by the change in the total number of nucleated cells in bone marrow and spleen, endogenous spleen colony formation, and 30-day mouse lethality. In the spleen there were profound effects on spleen weight, total organ cellularity, and endogenous colony formation. The TCDO produced radiation dose-modifying factors between 1.4 and 1.5 for endogenous spleen colony formation in mice primed with 3 Gy and reirradiated 4 days after treatment. This stimulated cellular recovery resulted in an increased protection of mice from lethality caused by subsequent total-body irradiation by factors of 1.12-1.18. The results show that TCDO is a potent stimulator of cellular recovery in the spleen of sublethally irradiated mice.
Mason
K A
KA
Department of Experimental Radiotherapy, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Murphy
S
S
Milas
L
L
D000818:Animals; D002713:Chlorine / Q000494:pharmacology*; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000502:physiology / Q000528:radiation effects; D006413:Hematopoietic System / Q000187:drug effects / Q000528:radiation effects*; D051379:Mice; D008809:Mice, Inbred C3H; D010087:Oxides / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects; D014916:Whole-Body Irradiation; D014945:Wound Healing / Q000187:drug effects*
D010087:Oxides; D002713:Chlorine; C041955:tetrachlorodecaoxide