Inhibition of tumor cell invasion in the Boyden chamber assay by a mannosidase inhibitor, mannostatin A.
An alpha-mannosidase inhibitor, mannostatin A, from Streptoverticillium verticillus var. quintum inhibited chemotactic invasion of mouse B16/F10 melanoma cells in the Boyden chamber assay. It also inhibited in vitro invasion of K-ras-NIH3T3 cells. Mannostatin A did not inhibit the growth of either cell line at the concentration effective to inhibit invasion. Addition of mannostatin A to the cultured B16/F10 or K-ras-NIH3T3 cells inhibited cellular alpha-mannoside activity specifically. Mannostatin A-treated B16/F10 cells also showed decreased metastatic activity in vivo in C57Bl/6 mice.
Ochi
Y
Y
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Atsumi
S
S
Aoyagi
T
T
Umezawa
K
K
D016475:3T3 Cells; D000818:Animals; D003517:Cyclopentanes / Q000494:pharmacology*; D015194:Diffusion Chambers, Culture; D005260:Female; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D008361:Mannosidases / Q000037:antagonists & inhibitors*; D008545:Melanoma / Q000473:pathology* / Q000517:prevention & control / Q000556:secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured
D003517:Cyclopentanes; C060107:mannostatin A; D008361:Mannosidases