Protein oxidation in magnesium deficient rat brains and kidneys.
Magnesium deficiency produces a pro-oxidant systemic inflammation in rats. The purpose of these experiments was to determine if and when there is any oxidation of cellular proteins. We have found there is a significant increase in protein oxidation products, protein carbonyls, in both the brain and the kidney within 2 to 3 weeks on a magnesium deficient diet. These changes occur prior to any detectable changes in cellular glutathione, tissue damage or dysfunction. We conclude that oxidation of cellular proteins occur early in magnesium deficiency and may contribute to the tissue damage and loss of function observed in the later stages of the deficiency. This is the first demonstration of the time course of protein oxidation product development in magnesium deficient animals.
Stafford
R E
RE
Department of Medicine, George Washington University Medical Center, Washington, DC 20037.
Mak
I T
IT
Kramer
J H
JH
Weglicki
W B
WB
D000818:Animals; D001921:Brain / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000097:blood; D007668:Kidney / Q000378:metabolism*; D008274:Magnesium / Q000097:blood; D008275:Magnesium Deficiency / Q000097:blood / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013054:Spectrophotometry, Atomic; D013997:Time Factors
D009419:Nerve Tissue Proteins; D011506:Proteins; D006861:Hydrogen Peroxide; D008274:Magnesium