Determination of anabolic steroid 19-nor-testosterone in bovine serum by GC-SIM-MS.
Anabolic steroids extracted from bovine serum by means of organic solvents or solid-phase extraction techniques with C18 cartridges are not suitable for quantitative analysis by gas chromatography-mass spectrometry (GC-MS); therefore, they were extracted from serum by Extrelut columns (Merck; Germany), eluted with diethyl ether, and purified by a solid-phase extraction technique using amino (NH2) columns. The resulting clean extract was treated with heptafluorobutyric anhydride (HFBA); this formed a derivative suitable for GC-MS analysis. Using selected ion monitoring (SIM), quantities as small as 40 pg/mL can be detected.
Perona
M
M
Analysis Laboratory, Sant'Anna Hospital, Turin, Italy.
Pavan
I
I
D000818:Animals; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009277:Nandrolone / Q000097:blood*
D009277:Nandrolone