title: HIV-1 gp41 binding to human peripheral blood mononuclear cells occurs preferentially to B Lymphocytes and monocytes.
abstract: Based on our findings that HIV-1 gp41 independently of CD4 can bind to the human helper T lymphoid cell line H9, B cell line Raji and monocyte cell line U937, we characterized putative binding of HIV-1 gp41 to human peripheral blood lymphocytes (PBLs) and monocytes. Using flow cytometry (FACS), we demonstrated that the recombinant soluble HIV-1 gp41 (sgp41; Env amino acid 539-684) can bind to the normal human peripheral blood mononuclear cells (PBMCs), preferentially to B lymphocytes and monocytes independently of gp120-binding sites on CD4 molecules. This binding is dose-dependent. The HIV-1 sgp41 binds to blood B lymphocytes and monocytes more strongly than to T lymphocytes. By two-color flow cytometric analysis, we identified that sgp41 can bind 10% of CD4+ T lymphocytes, 11.9% of CD8+ T lymphocytes, 47% of CD19+ B lymphocytes and 44.2% of CD14+ monocytes.
authors.affiliation: Ludwig Boltzmann Institute for AIDS Research, Innsbruck, Austria.
authors.forename: Y H
authors.initials: YH
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Böck
authors.forename: R
authors.initials: R
authors.lastname: Vornhagen
authors.forename: F
authors.initials: F
authors.lastname: Steindl
authors.forename: H
authors.initials: H
authors.lastname: Katinger
authors.forename: M P
authors.initials: MP
authors.lastname: Dierich
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism / Q000382:microbiology*; D001665:Binding Sites; D015700:HIV Envelope Protein gp41 / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000378:metabolism / Q000382:microbiology*; D011485:Protein Binding; D015728:Receptors, HIV / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000378:metabolism / Q000382:microbiology
chemical_list: D015700:HIV Envelope Protein gp41; D015728:Receptors, HIV