Binding of plasma selenoprotein P to cell membranes.
Competitive binding assays were performed to determine the amount of binding of 75Se-labeled plasma selenoprotein P (PSP) to membranes from different rat tissues at physiologic pH. 75Se PSP for use as a ligand in the binding assays was labeled in vivo by injecting rats with 75Se selenious acid. PSP was obtained from plasma by salt precipitation and affinity and ion-exchange chromatography. Membranes for receptor assays were prepared from liver, kidney, testes, and brain of rats fed diets with either 0.01, 0.1, or 2 ppm selenium. At pH 7.4 PSP was bound differentially to tissues in the following order: brain > kidney > testes > liver. Specific binding of PSP to tissue membranes from rats fed the different levels of selenium increased with increasing amounts of dietary selenium. Saturation assays indicated apparent saturation of membrane receptors by the 75Se-labeled PSP. Another significant new finding was a 134-kDa complex of PSP and membrane receptor, identified by gel-filtration chromatography of cross-linked samples from binding assays. This provides evidence for a membrane receptor for PSP in rat tissues.
Wilson
D S
DS
Department of Food Science and Technology, University of California, Davis 95616.
Tappel
A L
AL
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D003432:Cross-Linking Reagents; D008297:Male; D009928:Organ Specificity / Q000502:physiology; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium; D051149:Selenoprotein P; D051140:Selenoproteins
D003432:Cross-Linking Reagents; D011506:Proteins; D051149:Selenoprotein P; D051140:Selenoproteins; D012643:Selenium