Identification of proteins immunologically related to vertebrate lamins in the nuclear matrix of the myxomycete Physarum polycephalum.
Lamin-like proteins have been identified as components of the nuclear matrix of the myxomycete Physarum polycephalum. A 67 kDa homologue was detected by immunoblotting of nuclear matrix proteins with affinity-purified anti-lamin antibodies of human autoimmune sera. A 65 kDa lamin B homologue was identified with polyclonal antibodies against turkey erythrocyte lamin B in nuclei and nuclear matrix of Physarum amoebae and plasmodia. Indirect immunofluorescence microscopy revealed that the 65 and 67 kDa proteins are localized in the nuclear lamina of plasmodial nuclei.
Lang
S
S
Department of Microbiology, University of Innsbruck Medical School, Austria.
Loidl
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D005656:Fungal Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D034921:Lamin Type B; D034882:Lamins; D015530:Nuclear Matrix / Q000737:chemistry*; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology; D010804:Physarum / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D014422:Turkeys / Q000097:blood*
D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D007106:Immune Sera; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins