The tet(K) gene of plasmid pT181 of Staphylococcus aureus encodes an efflux protein that contains 14 transmembrane helices.
The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that the Tet(K) protein contains 14 transmembrane alpha-helices.
Guay
G G
GG
Department of Microbial Genetics, Lederle Laboratories, Pearl River, New York 10965.
Khan
S A
SA
Rothstein
D M
DM
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011815:R Factors; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism*; D013753:Tetracycline Resistance / Q000235:genetics*
D001426:Bacterial Proteins; D008565:Membrane Proteins; C084212:tet(K) protein, Staphylococcus aureus