A simple and rapid method for staining the enteric ganglia: application for Hirschsprung's disease.
A simple and reliable method is described for staining the nerve plexuses of the human gastrointestinal tract in whole-mounts. The staining does not require fixation and consists of a single step of 6 to 10 minutes incubation in a solution of the fluorescent dye 4-(4-diethylaminostyryl)-methylpyridinium iodide. Aganglionic bowel specimens from 6 patients with Hirschsprung's disease were stained by this method and displayed the absence of ganglion cells and an abundance of nerve fibers in the myenteric and submucosal plexuses. This staining may be suitable for the diagnosis of Hirschsprung's disease.
Hanani
M
M
Laboratory of Experimental Surgery, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel.
Udassin
R
R
Ariel
I
I
Freund
H R
HR
D002675:Child, Preschool; D005456:Fluorescent Dyes; D006627:Hirschsprung Disease / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D009197:Myenteric Plexus / Q000473:pathology*; D011726:Pyridinium Compounds; D013368:Submucous Plexus / Q000473:pathology*; D013997:Time Factors
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C052247:4-(4-diethylaminostyryl)-N-methylpyridinium