title: Ovarian dysplasia: nuclear texture analysis.
abstract: BACKGROUND
  Ovarian dysplasia has been defined by histologic and morphometric studies focusing on architectural and nuclear profile changes. A new technique is used to enhance the accuracy of this diagnosis by a quantitative evaluation of the nuclear texture that represents the nuclear chromatin pattern on which conventional diagnoses of malignancy are usually made.
  
  
  METHODS
  Histologic sections from 35 ovaries classified as malignant (12), dysplastic (12), and normal (11) were evaluated by tracing boundaries of nuclear profiles and measuring "textons" (texture primitives) with a histogram analysis of three zones of gray level densities (called for simplification white, gray, and dark). The average combined area was tabulated for specimens with the same diagnosis, and linear regression plots compared the texton area with total nuclear area.
  
  
  RESULTS
  The dimensions of textons originally hidden inside the chromatin and revealed by histograms were found to be closely clustered in normal epithelium, and increasingly dissociated from the containing nucleus as the lesion progressed from dysplastic to malignant. The statistical multivariate analysis including nine parameters correctly classified the three diagnostic categories as normal, dysplastic, and malignant.
  
  
  CONCLUSIONS
  Computerized image analysis of nuclear texture adds accuracy to the recently elaborated morphometric methods to define ovarian dysplasia, a potential precursor of ovarian carcinoma.
authors.affiliation: Department of Pathology, Mount Sinai School of Medicine, New York, New York.
authors.forename: L
authors.initials: L
authors.lastname: Deligdisch
authors.forename: C
authors.initials: C
authors.lastname: Miranda
authors.forename: J
authors.initials: J
authors.lastname: Barba
authors.forename: J
authors.initials: J
authors.lastname: Gil
mesh_terms: D002467:Cell Nucleus / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D003116:Color; D018283:Cystadenocarcinoma, Papillary / Q000473:pathology / Q000648:ultrastructure; D003937:Diagnosis, Differential; D004848:Epithelium / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D015999:Multivariate Analysis; D010051:Ovarian Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D010053:Ovary / Q000473:pathology* / Q000648:ultrastructure; D011336:Probability; D012044:Regression Analysis
chemical_list: D002843:Chromatin