High susceptibility to aflatoxin B1 and benzo[a]pyrene of BALB3T3 A31-1-1 cells expressing monkey CYP1A1.
The monkey CYP1A1 has been expressed in BALB 3T3 A31-1-1 cells and the expressed proteins were assayed for their capacity to activate aflatoxin B1 (AFB1) and benzo[a]pyrene (B[a]P). The transformed cells were approximately 5.4- and 4.7-fold more sensitive to AFB1 and B[a]P than the parental cells, respectively. These results indicate that monkey CYP1A1 cDNA encoded a functional protein and that the expressed CYP1A1 enzyme is active for the activation of B[a]P as well as AFB1 to produce toxic metabolites.
Itoh
S
S
Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
Tagawa
S
S
Sawada
M
M
Kamataki
T
T
D016475:3T3 Cells; D016604:Aflatoxin B1 / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000502:physiology*; D008252:Macaca fascicularis; D051379:Mice; D014162:Transfection
D001564:Benzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1