Direct demonstration of the CD27 molecule involved in the negative regulatory effect on T cell activation.
CD27 is a member of the nerve growth factor/tumor necrosis factor receptor family and appears to play an important role in the process of T cell activation. We have previously shown that perturbation of the CD27 molecule by anti-1A4 (CD27) mAb inhibits T cell proliferation induced by various stimuli. To further determine the molecular basis of this inhibition of T cell proliferation by anti-1A4 mAb, we have established CD27-transfectant lines. Introduction of the human CD27 cDNA to ovalbumin-specific I-Ad-restricted murine T cell hybridomas significantly reduced their antigen-specific IL-2 production. In addition, treatment of these human CD27 transfectants with an anti-CD27 mAb (anti-1A4) further reduced their IL-2 production. These results provide direct evidence that CD27 plays a crucial role in the process of T cell activation.
Sugita
K
K
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Tanaka
T
T
Doshen
J M
JM
Schlossman
S F
SF
Morimoto
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002645:Chickens; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D007376:Interleukin-2; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7