Induction of embryonic major histocompatibility complex antigen expression by gamma-IFN.
Preimplantation mouse embryos were incubated in vitro with mouse recombinant gamma-interferon (IFN). The effect of the gamma-IFN on major histocompatibility complex (MHC) class I antigen expression was tested using an ELISA procedure. It was found that there is a doubling of Db antigens and a tripling of Qa-2 antigens on C57BL/6 mouse embryos cultured from the 8-cell stage for 24 h in the presence of 10(5) units/ml gamma-IFN. The effect of gamma-IFN on the rate of preimplantation embryonic development was tested by culturing 2-cell embryos for 48 h and 8-cell embryos for 24 h in the presence of varying concentrations of gamma-IFN up to 10(6) units/ml. Two methods were used to assess the cell number per embryo after the culture period: incorporation of [3H]thymidine into DNA, and direct counting of nuclei in fixed and stained embryos. Both methods showed that treatment with gamma-IFN increases the rate of development of preimplantation mouse embryos. Since rate of preimplantation embryonic development is genetically controlled by the Ped gene, it is suggested that gamma-IFN has a direct effect on the Ped gene phenotype of preimplantation mouse embryos.
Warner
C M
CM
Department of Biology, Northeastern University, Boston, MA 02115.
Almquist
C D
CD
Toulimat
M H
MH
Xu
Y
Y
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005805:Genes, MHC Class I / Q000187:drug effects; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000196:embryology / Q000235:genetics / Q000276:immunology; D010641:Phenotype; D011994:Recombinant Proteins
D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I; C032907:Q surface antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma