Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi.
The products of RAPD-PCR amplification of Phytophthora cinnamomi DNA were separated by electrophoresis in agarose. Parallel Southern blots of the gels were hybridized with nick translated DNA from different species of Phytophthora. Fragments that hybridized specifically to P. cinnamomi DNA were identified. These fragments were purified and cloned into pUC18. Their specificity for P. cinnamomi was confirmed.
Dobrowolski
M P
MP
Biotechnology Programme, School of Biological and Environmental Sciences, Murdoch University, Australia.
O'Brien
P A
PA
D001483:Base Sequence; D015342:DNA Probes / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010838:Phytophthora / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
D015342:DNA Probes; D004271:DNA, Fungal