Use of nonradioactive DNA probes to identify a Campylobacter jejuni strain causing abortion.
A case of human abortion due to a Campylobacter infection is reported. Cultures revealed two morphologically different isolates with large and small colonies respectively. Using conventional methods of identification, the large colonies were identified as Campylobacter jejuni and the small colonies as Campylobacter coli. Dot blot hybridization and determination of rDNA restriction fragment patterns revealed that both colony types were the same strain of Campylobacter jejuni. This observation illustrates the need to use methods other than phenotypic methods when identifying strains of Campylobacter.
Quentin
R
R
Département de Microbiologie Médicale et Moleculaire, Centre Hospitalier Universitaire Bretonneau, Tours, France.
Chevrier
D
D
Guesdon
J L
JL
Martin
C
C
Pierre
F
F
Goudeau
A
A
D000022:Abortion, Spontaneous / Q000382:microbiology*; D000328:Adult; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D011247:Pregnancy
D015342:DNA Probes; D004269:DNA, Bacterial