Characterization of two marker chromosomes in a patient with acute nonlymphocytic leukemia by two-color fluorescence in situ hybridization.
A patient with acute nonlymphocytic leukemia (ANLL), M5b according to French-American-British (FAB) classification, showed monosomy 16, an extra 1p-, and a 21q+. These derivative chromosomes could not be defined by GTG-banding. For better characterization, we performed two-color fluorescence in situ hybridization (FISH) experiments applying DNA libraries from sorted human chromosomes, chromosome-specific repetitive probes, and a band-specific YAC-clone. With these FISH studies the karyotype could be characterized as 46,XY, +der(1)t(1;21)(p11;?), -16,der(21)t(16;21) (p11.1;q22).
Taniwaki
M
M
Institut für Humangenetik und Anthropologie, Universität Heidelberg, Germany.
Speicher
M R
MR
Lengauer
C
C
Jauch
A
A
Popp
S
S
Cremer
T
T
D000328:Adult; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D018244:Chromosomes, Artificial, Yeast; D002878:Chromosomes, Human, Pair 1; D002885:Chromosomes, Human, Pair 16; D002891:Chromosomes, Human, Pair 21; D015342:DNA Probes; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D009006:Monosomy; D014178:Translocation, Genetic
D015342:DNA Probes; D005819:Genetic Markers