title: Microbial conversion of avermectins by Saccharopolyspora erythraea: glycosylation at C-4' and C-4''.
abstract: Avermectins and ivermectins are glycosylated at C-4'' and C-4' by both growing and resting cells of Saccharopolyspora erythraea. The reaction is catalyzed by a glycosyltransferase which is constitutive. The enzyme uses UDP-glucose as the glycosyl donor and avermectin or ivermectin mono- and disaccharides as acceptors. Avermectin and ivermectin aglycones and erythromycin A are not substrates for the enzyme.
authors.affiliation: Merck Research Laboratories, Rahway, New Jersey 07065.
authors.forename: M
authors.initials: M
authors.lastname: Schulman
authors.forename: P
authors.initials: P
authors.lastname: Doherty
authors.forename: B
authors.initials: B
authors.lastname: Arison
mesh_terms: D000871:Anthelmintics / Q000378:metabolism*; D001711:Biotransformation; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism; D016695:Glycosyltransferases / Q000302:isolation & purification / Q000378:metabolism; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D016930:Saccharopolyspora / Q000187:drug effects* / Q000201:enzymology; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
chemical_list: D000871:Anthelmintics; D004187:Disaccharides; D006027:Glycosides; D007559:Ivermectin; C019264:avermectin; D016695:Glycosyltransferases; D014532:Uridine Diphosphate Glucose