Generation and characterization of monoclonal antibodies to porcine zona pellucida-3 alpha glycoprotein.
All 10 monoclonal antibodies generated reacted with deglycosylated ZP3 alpha both in ELISA and Western blots, thereby suggesting that these do not recognize carbohydrate determinants. Moreover, 7 antibodies (MAs -402, -403, -405, -412, -420, -421 and -423) recognized reduced and carboxyamidomethylated ZP3 alpha in Western blot suggesting that these antibodies recognized linear epitopes. The epitopes recognized by MAs -410, -413 and -425 were sequential or conformational, stabilized by disulphide bonds. Three antibodies namely, MA -405, -420 and -421 inhibited in vitro, zona lysis by trypsin.
Chadha
K
K
Gamete Antigen Laboratory, National Institute of Immunology, New Delhi, India.
Gupta
M
M
Gupta
S K
SK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D004527:Egg Proteins; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D011956:Receptors, Cell Surface; D013552:Swine; D015044:Zona Pellucida / Q000276:immunology*; D000072216:Zona Pellucida Glycoproteins
D000911:Antibodies, Monoclonal; D000941:Antigens; D004527:Egg Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C000606521:Zp3 protein, mouse