Interconnection between thyroid hormone signalling pathways and parvovirus cytotoxic functions.
Nonstructural (NS) proteins of autonomous parvoviruses can repress expression driven by heterologous promoters, an activity which thus far has not been separated from their cytotoxic effects. It is shown here that, in transient transfection assays, the NS-1 protein of the parvovirus minute virus of mice (MVMp) activates the promoter of the human c-erbA1 gene, encoding the thyroid hormone (T3) receptor alpha. The endogenous c-erbA1 promoter is also a target for induction upon MVMp infection. Moreover, T3 was found to up-modulate the level of cell sensitivity to parvovirus attack. These data suggest an interconnection between T3 signalling and NS cytotoxic pathways.
Vanacker
J M
JM
Unité d'Oncologie Moléculaire, Institut Pasteur de Lille, Centre National de la Recherche Scientifique URA 1160, France.
Laudet
V
V
Adelmant
G
G
Stéhelin
D
D
Rommelaere
J
J
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D008915:Minute Virus of Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000235:genetics*; D015398:Signal Transduction; D014162:Transfection; D014284:Triiodothyronine / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D017361:Viral Nonstructural Proteins; D014284:Triiodothyronine