Determination of erythromycin A by liquid chromatography and electrochemical detection, with application to salmon tissue.
The chromatographic performance of erythromycin A (EA) is improved significantly over that achieved on polymeric columns by using a sterically shielded octyldiisopropylsilica (Zorbax Rx-C8) column and a neutral mobile phase consisting of 5 mM aqueous sodium perchlorate-acetonitrile (50:50). This mobile phase facilitates electrochemical detection of EA at the 3-pmol level. Temperature control of both column and detection cell is important for minimizing detector noise and drift. A clean-up procedure, based on aminopropylsilica solid-phase extraction, allows the detection of EA in salmon flesh down to the 0.2-ppm level. Some of the metabolites of EA that retain the tertiary amine may also be detected by this method.
Janecek
M
M
Institute for Marine Biosciences, National Research Council, Halifax, Nova Scotia, Canada.
Quilliam
M A
MA
Bailey
M R
MR
North
D H
DH
D000818:Animals; D002853:Chromatography, Liquid; D004563:Electrochemistry; D004917:Erythromycin / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D012474:Salmon; D014131:Trace Elements
D014131:Trace Elements; D004917:Erythromycin