title: Effects of calcitriol and its analogue calcipotriol on proliferation and differentiation of human osteosarcoma cells.
abstract: The effects of steroid hormone calcitriol (Cal) and its analogue calcipotriol on human osteosarcoma cell line HOS-8603 were determined. When cells grew in monolayer culture in the presence of hormones, their proliferations were inhibited both in dose- and time-dependent manners. The cells showed marked morphologic changes after a 4-d treatment to apparently less transformed fibroblast-like ones. Anchorage-independent growth studies indicated that both Cal and calcipotriol at 10 nmol.L-1 inhibited colony formation by HOS-8603 cells. As a marker enzyme of the osteoblastic phenotype, alkaline phosphatase activity was induced in response to Cal or calcipotriol 100 nmol.L-1. These results suggested that Cal and calcipotriol play an important role in regulating growth and differentiation of HOS-8603 cells.
authors.affiliation: Department of Pathophysiology, Second Military Medical University, Shanghai, China.
authors.forename: L N
authors.initials: LN
authors.lastname: Song
authors.forename: J S
authors.initials: JS
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Lu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; C055085:calcipotriene; D000469:Alkaline Phosphatase; D002117:Calcitriol