title: Trimeric mutant bacteriorhodopsin, D85N, shows a monophasic CD spectrum.
abstract: The structure of mutant bacteriorhodopsin (bR), D85N, was examined by CD and X-ray diffraction at pH 7. The absorption maximum of D85N at pH 7 is located at 605 nm, which is similar to the acid-blue form of wild-type bR. D85N shows a monophasic CD band, the maximum of which is at 575 nm, although the crystalline arrangement and the trimeric structure is maintained. The acid-blue form of wild-type bR shows a biphasic CD despite the similarity in absorption spectra.
authors.affiliation: Department of Biology, Faculty of Science, Osaka University, Toyonaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kataoka
authors.forename: K
authors.initials: K
authors.lastname: Mihara
authors.forename: H
authors.initials: H
authors.lastname: Kamikubo
authors.forename: R
authors.initials: R
authors.lastname: Needleman
authors.forename: J K
authors.initials: JK
authors.lastname: Lanyi
authors.forename: F
authors.initials: F
authors.lastname: Tokunaga
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D014961:X-Ray Diffraction
chemical_list: D001436:Bacteriorhodopsins