Radioimmunodetection of rat and rabbit cartilage using a monoclonal antibody specific to link proteins.
Biodistribution analysis using [125I]Fab-6F3 specific to link proteins from human articular cartilage performed in rats by autoradiography showed a high concentration of radioactivity in all cartilaginous tissues. Preliminary immunoscintigraphic assays were performed in rabbits. Front and side view images of whole animals exhibited high uptake in cartilage tissue of the knee articulation, in the invertebral disk and the humeral head. This fixation was still detected 24 h post-injection, although high washout of radioactivity was observed.
Cassiede
P
P
INSERM-U. 306, Université de Bordeaux II, France.
Amedee
J
J
Vuillemin
L
L
Rouais
F
F
Bareille
R
R
Bordenave
L
L
Basse-Cathalinat
B
B
Harmand
M F
MF
Ducassou
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001345:Autoradiography; D002356:Cartilage / Q000000981:diagnostic imaging*; D016326:Extracellular Matrix Proteins; D007140:Immunoglobulin Fab Fragments; D008297:Male; D011506:Proteins / Q000276:immunology*; D011509:Proteoglycans / Q000276:immunology*; D011817:Rabbits; D016719:Radioimmunodetection; D051381:Rats; D017208:Rats, Wistar; D000099249:Proteoglycan Link Protein
D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D007140:Immunoglobulin Fab Fragments; D011506:Proteins; D011509:Proteoglycans; D000099249:Proteoglycan Link Protein