Specific nitrogen-15 labelling of leucine residues in human growth hormone.
Biosynthetic human growth hormone (hGH) specifically 15N labelled in the leucine residues has been obtained by recombinant DNA technology, using 15N-labelled leucine and an E. coli strain that requires leucine. It is shown that, despite the possibility of minor transaminase activity, the labelling on the whole is specific, and that the two-dimensional 1H-15N correlation NMR spectra of hGH can be greatly simplified by this methodology.
Christensen
T
T
Novo Nordisk A/S, Novo Allé, Bagsvaerd, Denmark.
Petersen
J
J
Theisen
C F
CF
Bjerregaard
K
K
Kristensen
S M
SM
Led
J J
JJ
D004926:Escherichia coli / Q000235:genetics; D013006:Growth Hormone / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007930:Leucine / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes
D009587:Nitrogen Isotopes; D013006:Growth Hormone; D007930:Leucine