Heterologous protein secretion directed by a repressible acid phosphatase system of Aspergillus niger.
A new expression-secretion system of Aspergillus niger which directs the secretion of heterologous proteins is described. The promoter and signal peptide-encoding region of the phosphate-repressible aphA gene of A. niger, when fused to the coding region of the human interferon alpha 2 (hIFN alpha 2)-encoding gene (hIFN alpha 2), drives the expression of this gene and the secretion of the hIFN alpha 2 protein. Synthesis of hIFN alpha 2 in either A. niger or A. nidulans transformants carrying these constructs was regulated by inorganic phosphate (Pi) present in the medium, so that derepression of heterologous protein expression can be attained by lowering Pi concentration.
MacRae
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WD
Allelix Biopharmaceuticals Inc., Mississauga, Ontario, Canada.
Buxton
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FP
Gwynne
D I
DI
Davies
R W
RW
D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005822:Genetic Vectors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D015183:Restriction Mapping; D014170:Transformation, Genetic
D004271:DNA, Fungal; D007370:Interferon Type I; D010710:Phosphates; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D000135:Acid Phosphatase