Insertion of transposon Tn5seq1 into G+C-rich DNA of Streptomyces avermitilis: generation of 8-, 9-, and 10-bp duplications.
The sites for 24 Tn5seq1 insertions into G+C-rich DNA of Streptomyces avermitilis were determined. Although Tn5-based transposons usually generate a 9-bp duplication at their insertion site, one Tn5seq1 insertion generated a 10-bp duplication and another insertion an 8-bp duplication.
Occi
J L
JL
Merck Research Laboratories, Rahway, New Jersey 07065.
Gibbons
P H
PH
Wong
E
E
MacNeil
D J
DJ
D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial