Swelling-stimulated passive potassium transport in camel erythrocytes: inhibitory effects of furosemide and sodium fluoride.
The inhibitory effects of furosemide, sodium fluoride, and age on volume-dependent, ouabain-resistant K+ influx were investigated in camel red blood cells. Swelling of young camel erythrocytes hypotonically stimulates ouabain-resistant potassium influx, a response that was lacking in old camel erythrocytes. The swelling-stimulated influx was partially inhibited by 1 mM furosemide and by 10 and 20 mM sodium fluoride. The inhibitory effect of furosemide was significantly increased if rubidium was added to the flux media. There was a significant correlation between potassium influx in normo- and hypotonic media which might indicate that the anion-dependent transport system operates, to some extent, to regulate cell volume.
Gharaibeh
N S
NS
Department of Physiology and Biochemistry, Jordan University of Science and Technology, Irbid.
Rawashdeh
N M
NM
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002162:Camelus; D004911:Erythrocyte Volume / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology*; D066298:In Vitro Techniques; D011188:Potassium / Q000378:metabolism*; D012969:Sodium Fluoride / Q000494:pharmacology*
D005665:Furosemide; D012969:Sodium Fluoride; D011188:Potassium