[Antigen analysis of McAb E4B7D5 directed against outer envelope of Leptospira interrogans serovar lai by SDS-PAGE and immunoblot].
McAb E4B7D5 was prepared by hybridoma technology in BALB/c mice immunized to outer envelope of Leptospira interrogans serovar lai. This McAb agglutinated specifically with all the 13 serovars of Icterohaemorrhagiae serogroup in MAT test at high titres and protected the guinea pigs against the attack of virulent strain (017) of serovar lai. SDS-PAGE and immunoblot were used to analyse the reaction of the outer envelopes of the five strains of Leptospira (Leptospira interrogans Icterohaemorrhagiae serogroup, serovar lai, 017 and 601 stains; L. interrogans Hebdomadis serogroup, hebdomadis serovar strain 156; L. interrogans Australis serogroup australis serovar strain 620; biflexa leptospira patoc serovar strain Patoc I; with McAb E4B7D5. Results indicated that this McAb E4B7D5 recognized specifically 34.5 kd and 39.5 kd outer envelope proteins of serovar lai, strain 017 and 601, so it might be an immunoprotective McAb directed against Leptospira interrogans serovar lai. It was suggested that 34.5 kd and 39.5 kd outer envelope proteins of serovar lai recognized specifically by McAb E4B7D5 might be immunoprotective antigens.
Li
Y
Y
Dai
B
B
Zhao
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins