title: [In vitro study of the sensitivity to antibiotics of 213 Pseudomonas aeruginosa strains isolated in an intensive care unit of the Sfax Hospital (Tunisia)].
abstract: Susceptibility of 213 strains of Pseudomonas aeruginosa isolated in a general intensive care unit during two years 1989 and 1990 was tested against 11 antibiotics: ticarcillin (TIC), ticarcillin+clavulanic acid (TCC), cefsulodin (CFS), ceftazidime (CAZ), imipenem (IMP), gentamicin (G), tobramycin (TOB), amikacin (AN), ofloxacin (OFX), norfloxacin (NOR) and ciprofloxacin (CIP). Antibiotic susceptibility testing was performed by disk agar diffusion test and by measuring minimum inhibitory concentrations (MICS using test agar dilution. 65% of strains were isolated from respiratory tract infections. Resistance frequency of this antibiotics was: TIC: 41.3%, TCC: 33.8%, CFS: 26.5%, CAZ: 23.2%, IMP: 11.6%, G: 72.5%, TOB: 46.5%, AN: 12.6%, OFX: 29.3%, NOR: 21.3%, CIP: 17.6%. We observed by measuring CMI that imipenem and amikacin were the most active antibiotics. From 1989 et 1990, the increase number of strains resistant to imipenem, amikacin and fluoroquinolones could be explained by massive use of those antibiotics. Besides their high level of resistance, strains isolated in intensive care unit ere characterized by their multiresistance: 24 strains were resistant to 8 of 11 antibiotics tested; four of them showed resistance to all antibiotics the multiresistance of those strains suggest strongly that decreased permeability could be the cause.
authors.affiliation: Laboratoire de Microbiologie (Pr A. Hammami), CHU Habib Bourguiba, SFAX, Tunisie.
authors.forename: H
authors.initials: H
authors.lastname: Karray
authors.forename: A
authors.initials: A
authors.lastname: Hammami
authors.forename: F
authors.initials: F
authors.lastname: Mahjoubi
authors.forename: M
authors.initials: M
authors.lastname: Bouaziz
authors.forename: J
authors.initials: J
authors.lastname: Damak
authors.forename: H M
authors.initials: HM
authors.lastname: Jeddi
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D024841:Fluoroquinolones; D006801:Humans; D066298:In Vitro Techniques; D007362:Intensive Care Units; D007769:Lactams; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014416:Tunisia
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D007769:Lactams
vernacular_title: Etude in vitro de la sensibilité aux antibiotiques de 213 souches de Pseudomonas aeruginosa isolées au service de réanimation du CHU de Sfax (Tunisie).