Protein blotting analysis of large sample numbers.
A method is described that allows probing of hundreds of different antibodies on protein blots per day, making it possible to use protein blots for primary screening of hybridoma supernatants. It involves a miniaturization of the protein blots and their attachment to a solid support. This allows a great reduction of the required number of gels and electroblots and brings a drastic simplification of the incubation and washing procedures. Apart from a reduced resolution of closely adjacent bands the resulting mini-blots are comparable to conventional protein blots in sensitivity and specificity. The procedure has been successfully applied for primary screening of hybridoma supernatants. It can be useful in cases where a monoclonal antibody to an antigen with known molecular weight is required, or in situations where many different antibodies have to be tested in a short time period.
Kapfhammer
J P
JP
Brain Research Institute, University of Zurich, Switzerland.
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000941:Antigens / Q000276:immunology; D004586:Electrophoresis / Q000706:statistics & numerical data; D006825:Hybridomas / Q000276:immunology; D008567:Membranes, Artificial; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens; D008567:Membranes, Artificial; D011506:Proteins