title: Pyrularia thionin binding to and the role of tryptophan-8 in the enhancement of phosphatidylserine domains in erythrocyte membranes.
abstract: Pyrularia thionin is a small, strongly basic peptide which interacts readily with cellular and synthetic membranes. With cells it induces hemolysis, depolarizes the cellular membrane with an accompanying influx of Ca2+, and activates an endogenous phospholipase A2. Evidence points toward a binding site involving phosphatidylserine (PS). This study shows that addition of the peptide to erythrocyte membranes as well as to vesicles formed from phospholipids isolated from erythrocyte membranes causes an enhancement of phospholipid domains which are made visible by the use of fluorescence digital imaging microscopy with fluorescent derivatives of PS (NBD-PS) and phosphatidylcholine (NBD-PC). Addition of thionin caused a large increase in NBD-PS domains, with an accompanying enrichment of NBD-PC in another separate domain. Double-labeling experiments performed with a Texas Red derivative of thionin show that the peptide binds to the domain enriched in NBD-PS. P thionin inactivated by modification of Trp-8 with N-bromosuccinimide lost the ability to enhance PS domains, although it bound to the membrane with the same affinity as native P thionin. This shows that binding to the membrane is not in itself sufficient to cause the NBD-PS and NBD-PC redistribution into domains.
authors.affiliation: Department of Biochemistry, University of Illinois, Urbana 61801.
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: G H
authors.initials: GH
authors.lastname: Naisbitt
authors.forename: L P
authors.initials: LP
authors.lastname: Vernon
authors.forename: M
authors.initials: M
authors.lastname: Glaser
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides; D003603:Cytotoxins / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D010713:Phosphatidylcholines / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D010947:Plants, Toxic; D011817:Rabbits; D014364:Tryptophan / Q000737:chemistry
chemical_list: D023181:Antimicrobial Cationic Peptides; D003603:Cytotoxins; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D010940:Plant Proteins; C044942:THI1 protein, Pyrularia pubera; D014364:Tryptophan