Non-radioisotope labelling of DNA with multifunctional labelling agent.
A novel bi-functional fluorescent labelling agent derived from 5-hydroxy-1-naphthalene sulfonic acid and tris(2-amino-ethyl)amine was prepared and incorporated to 5'-monophosphate function of DNA through phosphoramidate bond. The labelling agent was designed to bear fluorescent moiety as well as primary and tertiary amines which are expected to have positive charge at neutral pH range. A 15-mer DNA labelled with this reagent exhibited enhanced duplex forming ability with its complement compared to the corresponding un-labelled DNA.
Shinozuka
K
K
Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu City, Japan.
Seto
Y
Y
Kawata
H
H
Sawai
H
H
D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004048:Diethylamines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009282:Naphthalenesulfonates / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D003432:Cross-Linking Reagents; D004048:Diethylamines; D005456:Fluorescent Dyes; D009282:Naphthalenesulfonates; C084407:5-(2-(bis(2-aminoethyl)amino)ethylamino)naphthalene-1-sulfonic acid; D004247:DNA