Histochemical survey of the anterior segment of the normal human foetal and adult eye.
The distributions of the lysosomal enzymes [acid phosphatase (AP), N-acetyl-beta-D-glucosaminidase (NAG), beta-glucuronidase (beta-Gluc), beta-galactosidase (beta-Gal), dipeptidylpeptidase II (DPP II)] and of the membrane-bound proteases [aminopeptidase M (APM), aminopeptidase A (APA), gamma-glutamyltransferase (GGT), dipeptidylpeptidase IV (DPP IV)] were investigated in the normal human adult and foetal anterior segment by histochemical methods. The distribution of these hydrolases varied between ocular tissues. The most active enzymes in the adult corneal epithelium and endothelium were AP, beta-Gluc, NAG, beta-Gal and GGT; in the keratocytes, APM, APA, beta-Gluc and GGT predominated. The adult trabecular meshwork cells were stained by AP, beta-Gluc, NAG, APM, GGT, DPP II and DPP IV. The enzymes AP, beta-Gluc, APM and APA, however, displayed greater activity in the endothelium of Schlemm's canal. The adult ciliary epithelium stained strongly for all lysosomal hydrolases; GGT was the most active protease here. Differences in enzyme activity were noted in some tissues when foetal and adult anterior segments were compared. There appeared to be a decrease in the activity of some enzymes with age and post-mortem delay greater than 24 h. The function(s) of each enzyme and their possible roles in the respective tissues are discussed.
Coupland
S E
SE
Department of Clinical Ophthalmology, Save Sight and Eye Health Institute, University of Sydney, NSW, Australia.
Penfold
P L
PL
Billson
F A
FA
D000368:Aged; D000369:Aged, 80 and over; D000869:Anterior Eye Segment / Q000196:embryology* / Q000201:enzymology / Q000378:metabolism*; D002924:Ciliary Body / Q000378:metabolism; D003315:Cornea / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D005865:Gestational Age; D006651:Histocytochemistry; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007498:Iris / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D014129:Trabecular Meshwork / Q000378:metabolism; D014166:Transferases / Q000378:metabolism*
D008565:Membrane Proteins; D014166:Transferases; D006867:Hydrolases