Definition of the Th/To ribonucleoprotein by RNase P and RNase MRP.
We show that the Th/To ribonucleoprotein is defined by (i) the co-immunoprecipitation of two RNAs, (ii) the co-immunoprecipitation of four major polypeptides and (iii) the quantitative immune recognition of both RNase P and RNase MRP. No serum was found that recognizes either one of these two enzymes exclusively. The specific co-immunoprecipitation of RNase MRP and RNase P by all Th/To ribonucleoprotein autoantibodies indicates that the anti-Th/To autoimmune response is directed against both enzymes in a quantitatively indistinguishable manner. Thus the Th/To ribonucleoprotein is defined by RNase P and RNase MRP.
Rossmanith
W
W
Institut für Tumorbiologie-Krebsforschung der Universität Wien, Austria.
Karwan
R
R
D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology; D004722:Endoribonucleases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D011233:Precipitin Tests; D016337:RNA, Catalytic / Q000378:metabolism*; D043262:Ribonuclease P; D012261:Ribonucleoproteins / Q000276:immunology / Q000378:metabolism*
D001323:Autoantibodies; D016337:RNA, Catalytic; D012261:Ribonucleoproteins; D004722:Endoribonucleases; C058330:mitochondrial RNA-processing endoribonuclease; C468368:RPP14 protein, human; D043262:Ribonuclease P