title: DNA adduct detection: some applications in monitoring exposure to environmental genotoxic chemicals.
abstract: In the assessment of genotoxic risk factors in the environment, the measurement of DNA adducts in aquatic organisms and in plants may have considerable implications. Using 32P-postlabelling, we have detected DNA adducts in the liver of carp (Chondrostoma nasus) from the River Rhône (France), both downstream and upstream from a polychlorinated biphenyl incineration plant. Some of the DNA adducts were specific to downstream fish, suggesting a differential pattern of exposure. We have also detected DNA damage in needles in a declining spruce forest. We found that, in the declining forest, the amounts of DNA adducts increase in relation to the degree of damage to the needles whereas, in a healthy forest, the levels of DNA adducts were low. We have also found DNA adducts in the leaves of hops grown in fields where heptachlor residues persisted.
authors.affiliation: Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, Strasbourg, France.
authors.forename: A
authors.initials: A
authors.lastname: Pfohl-Leszkowicz
authors.forename: F
authors.initials: F
authors.lastname: Weber-Lotfi
authors.forename: J F
authors.initials: JF
authors.lastname: Masfaraud
authors.forename: A
authors.initials: A
authors.lastname: Devaux
authors.forename: A
authors.initials: A
authors.lastname: Laouedj
authors.forename: P
authors.initials: P
authors.lastname: Guillemaut
authors.forename: C
authors.initials: C
authors.lastname: Malaveille
authors.forename: B
authors.initials: B
authors.lastname: Rether
authors.forename: G
authors.initials: G
authors.lastname: Monod
authors.forename: G
authors.initials: G
authors.lastname: Dirheimer
mesh_terms: D000818:Animals; D002347:Carps / Q000378:metabolism; D004247:DNA / Q000032:analysis* / Q000187:drug effects; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D008099:Liver / Q000737:chemistry; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D010944:Plants / Q000737:chemistry; D014197:Trees / Q000737:chemistry
chemical_list: D009153:Mutagens; D004247:DNA