title: The protein phosphatases responsible for dephosphorylation of hormone-sensitive lipase in isolated rat adipocytes.
abstract: The levels of the cytosolic serine/threonine protein phosphatases (PP) in rat adipocyte extracts have been determined, by using both reference substrates and hormone-sensitive lipase (HSL) as substrates. Adipocytes contain significant levels of both PP1 and 2A (1.6 and 2.0 m-units/ml of packed cells respectively), with lower levels of PP2C and virtually no PP2B activity. PP2A and 2C exhibit similar degrees of activity against HSL phosphorylated at site 1, together accounting for 92% of the total. In contrast, site 2 is dephosphorylated predominantly by PP2A (over 50% of total activity), whereas PP1 and PP2C contribute approx. 20% and 30% respectively to the total phosphatase activity against that site. Total phosphatase activity in the adipocyte extracts was 2-3-fold higher against site 2 than against site 1. The possible significance of these findings to the regulation of HSL activity in adipose tissue in vivo is discussed.
authors.affiliation: Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne U.K.
authors.forename: S L
authors.initials: SL
authors.lastname: Wood
authors.forename: N
authors.initials: N
authors.lastname: Emmison
authors.forename: A C
authors.initials: AC
authors.lastname: Borthwick
authors.forename: S J
authors.initials: SJ
authors.lastname: Yeaman
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D002417:Cattle; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D002787:Sterol Esterase / Q000378:metabolism*
chemical_list: D002787:Sterol Esterase; D010749:Phosphoprotein Phosphatases