Erythrocyte galactokinase assay with high performance liquid chromatography.
An assay for erythtocyte galactokinase based on high performance liquid chromatographic determination of galactose 1-phosphate (Gal-1-P) is described. The determination of Gal-1-P was applied to a post-column fluorometric detection of reducing sugars using arginine. This method is as sensitive and accurate as conventional radioisotopic methods, but needs no radioisotopic facilities. It requires only a small blood sample and is suitable as a follow-up test in neonatal screening.
Mizoguchi
N
N
Department of Pediatrics, Hiroshima University School of Medicine, Japan.
Eguchi
T
T
Sakura
N
N
Ueda
K
K
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000201:enzymology*; D005686:Galactokinase / Q000097:blood*; D005695:Galactosephosphates / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007231:Infant, Newborn; D013050:Spectrometry, Fluorescence
D005695:Galactosephosphates; D007202:Indicators and Reagents; D005686:Galactokinase