title: Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824.
abstract: The genes encoding both Clostridium acetobutylicum ATCC 824 butyrate synthesis pathway enzymes, phosphotransbutyrylase (ptb) and butyrate kinase (buk), were sequenced. The genes are immediately adjacent on the chromosome, with ptb preceding buk. A single transcription start point (tsp) was identified 57 bp upstream from the ptb start codon by primer extension analysis. The ptb and buk genes appear to form an operon. A putative Rho-independent terminator structure was identified 26 bp downstream from buk.
authors.affiliation: Department of Chemical Engineering, Northwestern University, Evanston, IL 60208.
authors.forename: K A
authors.initials: KA
authors.lastname: Walter
authors.forename: R V
authors.initials: RV
authors.lastname: Nair
authors.forename: J W
authors.initials: JW
authors.lastname: Cary
authors.forename: G N
authors.initials: GN
authors.lastname: Bennett
authors.forename: E T
authors.initials: ET
authors.lastname: Papoutsakis
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002087:Butyrates / Q000378:metabolism*; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D010709:Phosphate Acetyltransferase / Q000235:genetics*; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000235:genetics*; D014176:Protein Biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D002087:Butyrates; D004269:DNA, Bacterial; D010709:Phosphate Acetyltransferase; D017851:Phosphotransferases (Carboxyl Group Acceptor); C019285:butyrate kinase