Evidence that membrane proteins of rhabdomyosarcoma cell line RD bind human immunodeficiency virus type 1 (HIV-1).
Membrane proteins (MP) obtained from the human mesenchymal rhabdomyosarcoma cell line RD were coated on 96-well polystyrene microplates and tested for their ability to bind human immunodeficiency virus type 1 (HIV-1). The virus bound to MP was detected by solid phase assay. Anti-human CD4 monoclonal antibodies directed against the HIV-1 gp120 binding site of the CD4 receptor did not inhibit viral binding to MP. HIV-1 specific polypeptides were recovered from coated MP to microplates by a modification of the solid phase immunoisolation technique and shown by immunoblotting analysis using a high titer of biotinylated human anti-HIV-1 IgG. Together these findings provide evidence that HIV-1 binding to RD cell surfaces can proceed via a mechanism other than those mediated by the CD4 receptor.
Benzair
A B
AB
INSERM, Unité 322, Rétrovirus et Maladies Associées, Campus de Luminy, Marseille, France.
Hirsch
I
I
Chermann
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D015153:Blotting, Western; D015704:CD4 Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D011137:Polystyrenes; D012208:Rhabdomyosarcoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011137:Polystyrenes; D014764:Viral Proteins