title: Characterization of the nucleational core complex responsible for mineral induction by growth plate cartilage matrix vesicles.
abstract: The factors that drive mineralization of matrix vesicles (MV) have proven difficult to elucidate; in the present studies, various detergent, chemical, and enzyme treatments were used to reveal the nature of the nucleational core. Incubation with detergents that permeabilized the membrane enhanced calcification of treated MV incubated in synthetic cartilage lymph. While detergents removed most of the membrane lipid, they left significant amounts of the MV annexins and nearly all of the Ca2+, Pi, and Zn2+. Extraction with 1 M NaCl removed much of the Ca2+ and Pi present in MV, markedly reducing Ca2+ accumulation; these effects could be prevented by low levels of Ca2+ and Pi in the NaCl extractant. Treatment with chymotrypsin appeared to damage proteins required for MV mineralization; further treatment with detergents to bypass the membrane reactivated MV mineralization. Treatment of MV with pH 6 citrate removed Ca2+ and Pi, destroying their ability to mineralize; subsequent treatment with detergents did not reactivate these MV. Incubation of the detergent-resistant core with o-phenanthroline complexed Zn2+ and stimulated mineralization; addition of Zn2+ to synthetic cartilage lymph blocked the ability of the core to mineralize. These studies show that once the nucleational core complex is formed, the membrane-enclosed domain is no longer essential for MV calcification. Our findings indicate that the MV core contains two main components as follows: a smaller membrane-associated complex of Ca2+, Pi, phosphatidylserine, and the annexins that nucleates crystalline mineral formation, and a larger pool of Ca2+ and Pi bound to lumenal proteins. These proteins appear to bind large amounts of mineral ions, stabilize the nucleational complex, and aid its transformation to the first crystalline phase. Once nucleated, the crystalline phase appears to feed on protein-bound mineral ions until external ions enter through the MV ion channels. Zn2+ appears to regulate gating of the ion channels and conversion of the nucleational complex to the crystalline state.
authors.affiliation: Department of Chemistry and Biochemistry, School of Medicine, University of South Carolina, Columbia 29208.
authors.forename: L N
authors.initials: LN
authors.lastname: Wu
authors.forename: T
authors.initials: T
authors.lastname: Yoshimori
authors.forename: B R
authors.initials: BR
authors.lastname: Genge
authors.forename: G R
authors.initials: GR
authors.lastname: Sauer
authors.forename: T
authors.initials: T
authors.lastname: Kirsch
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: R E
authors.initials: RE
authors.lastname: Wuthier
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001857:Bone Matrix / Q000378:metabolism*; D002113:Calcification, Physiologic; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D002918:Chymotrypsin / Q000378:metabolism; D002951:Citrates / Q000494:pharmacology; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006132:Growth Plate / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D050356:Lipid Metabolism; D009994:Osmolar Concentration; D010618:Phenanthrolines / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: D002951:Citrates; D003902:Detergents; D010618:Phenanthrolines; D010710:Phosphates; D000469:Alkaline Phosphatase; D002918:Chymotrypsin; D015032:Zinc; D002118:Calcium; C025205:1,10-phenanthroline