Typing of Aeromonas spp. by numerical analysis of immunoblotted SDS-PAGE gels.
One hundred and three isolates of Aeromonas spp., collected from both environmental sources and patients, were examined by SDS-PAGE of whole cells followed by immunoblotting with polyclonal rabbit antiserum raised against whole cells of A. sobria. All isolates were typable, yielding 15-20 well separated bands. Reproducibility of the technique was good and discrimination excellent, yielding 30 types amongst 103 isolates. Immunoblot type was not related to biochemical phenotype. Attempts to correlate immunoblot type with serotype were unsuccessful because only 42% of the strains tested could be serotyped.
Mulla
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R
Department of Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Millership
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D000293:Adolescent; D000328:Adult; D000333:Aeromonas / Q000737:chemistry / Q000145:classification*; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002675:Child, Preschool; D016000:Cluster Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004783:Environmental Microbiology; D005260:Female; D005516:Food Microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D015151:Immunoblotting; D007223:Infant; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D015203:Reproducibility of Results; D012703:Serotyping
D001426:Bacterial Proteins