title: Dephosphorylation of photosystem II core proteins is light-regulated in vivo.
abstract: A number of photosystem II (PSII)-associated proteins, including D1, D2, CP43 and LHCII, are phosphorylated post-translationally by a membrane-bound, redox-regulated kinase activity. In vitro studies have demonstrated that these proteins can be dephosphorylated by membrane-bound phosphatase activity, reportedly insensitive to light or redox control. We demonstrate here that the PSII core proteins, D1, D2 and CP43, undergo light-stimulated, linear electron-transport-independent dephosphorylation in vivo. The in vivo dephosphorylation of D1 was characterized further and shown to depend upon light intensity, and to occur throughout the visible light spectrum with characteristics most consistent with light absorption by chlorophyll. PSII core protein dephosphorylation in vivo was stimulated by photosystem I (PSI)-specific far-red light, and inhibited by 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, an inhibitor of plastoquinol oxidation by the cytochrome b6f complex. Based on these findings, we propose that PSI excitation is involved in regulating dephosphorylation of PSII core proteins in vivo.
authors.affiliation: Plant Molecular Biology Laboratory, Beltsville Agricultural Research Center-West, MD 20705-2350.
authors.forename: T D
authors.initials: TD
authors.lastname: Elich
authors.forename: M
authors.initials: M
authors.lastname: Edelman
authors.forename: A K
authors.initials: AK
authors.lastname: Mattoo
mesh_terms: D003991:Dibromothymoquinone / Q000494:pharmacology; D004579:Electron Transport; D007700:Kinetics; D008027:Light; D045342:Light-Harvesting Protein Complexes; D010084:Oxidation-Reduction; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010944:Plants / Q000378:metabolism
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D003991:Dibromothymoquinone