Identification and characterization of an extended Tax protein in human T-cell lymphotropic virus type II subtype b isolates.
The tax gene sequence of human T-cell lymphotropic virus type II isolate G12 (HTLV-IIG12) was found to encode an extended Tax protein when compared with that of HTLV-IIMoT. In vitro transcription-translation of the HTLV-IIG12 tax gene produced a 40-kDa Tax protein that specifically reacted with serum specimens from HTLV-II-infected individuals. Limited sequence analysis demonstrated that isolates with an extended Tax protein were all HTLV-II subtype b (HTLV-IIb). Therefore, the extended Tax protein appears to be a unique characteristic of most HTLV-IIb isolates and may be useful in designing immunoassays to distinguish between HTLV-IIa and HTLV-IIb.
Pardi
D
D
Retrovirus Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Kaplan
J E
JE
Coligan
J E
JE
Folks
T M
TM
Lal
R B
RB
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002474:Cell-Free System; D016356:Gene Products, tax / Q000235:genetics* / Q000276:immunology; D016355:Genes, pX / Q000235:genetics*; D015491:HTLV-II Infections / Q000382:microbiology; D015367:Human T-lymphotropic virus 2 / Q000145:classification / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007198:Indians, North American; D007199:Indians, South American; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D000914:Antibodies, Viral; D016356:Gene Products, tax