Isolation and mapping of 45 NotI linking clones to chromosome 22.
Fifty-nine NotI linking clones have been isolated from a flow-sorted chromosome 22 cosmid library and mapped using fluorescence in situ hybridization and/or a panel of somatic cell hybrids. Fourteen clones map to the short arm of chromosome 22, 31 to the long arm, and 9 to other chromosomes; 5 clones could not be unambiguously mapped. To identify potentially informative genetic markers, the chromosome 22 clones were screened for poly(CA) sequences; 24 positively hybridizing clones, 10 on the long arm and 14 on the short arm, were identified. These clones will be useful for constructing a long-range restriction map of chromosome 22 and may facilitate the cloning of chromosome 22 genes.
Sanson
M
M
Centre for Research in Neuroscience, McGill University, Montreal, Canada.
Zhang
F
F
Demczuk
S
S
Delattre
O
O
DeJong
P
P
Aurias
A
A
Thomas
G
G
Rouleau
G A
GA
D001483:Base Sequence; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
D009838:Oligodeoxyribonucleotides; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases