cDNA cloning, expression during development, and genome mapping of PSY2, a second tomato gene encoding phytoene synthase.
The enzyme phytoene synthase (Psy) catalyzes the formation of phytoene, an intermediate in the carotenoid biosynthesis pathway. Expression of a previously described gene (PSY1) is induced by fruit ripening in tomato (Lycopersicon esculentum). We describe here the cloning of a partial cDNA for PSY2, a gene related to PSY1. A plasmid containing the PSY2 coding region under control of a bacterial promoter complements a Rhodobacter capsulatus phytoene synthase mutant, indicating that this gene has the capacity to encode an active enzyme. We used reverse transcriptase-polymerase chain reaction followed by digestion with a restriction enzyme to determine that both PSY1 and PSY2 are expressed during tomato development. PSY1 transcripts predominate in seedlings and in late stages of fruit ripening, whereas PSY2 transcripts are relatively more abundant in mature leaves. Both genes are expressed under photooxidative conditions induced by treatment with the carotenoid biosynthesis inhibitor Norflurazon. We used polymerase chain reaction-based restriction fragment length polymorphisms and alien addition lines to map PSY2 to chromosome 2. We conclude that PSY2 is a second tomato gene encoding phytoene synthase.
Bartley
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GE
E. I. Du Pont de Nemours and Company, Experimental Station, Wilmington, Delaware 19880-0402.
Scolnik
P A
PA
D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D003624:Darkness; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D005816:Genetic Complementation Test; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D007527:Isoenzymes / Q000235:genetics*; D008027:Light; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016354:Rhodobacter capsulatus / Q000235:genetics; D014166:Transferases / Q000235:genetics*; D014675:Vegetables / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D018076:DNA, Complementary; D007527:Isoenzymes; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase