A novel, sensitive radioimmunoprecipitation assay for the detection of antibodies to human immunodeficiency virus-type 2.
A radioimmunoprecipitation assay that provides an alternative to the Western blot assay was developed for characterizing antibodies against human immunodeficiency virus type-2 (HIV-2). The assay is based on radioiodination of antigen using Bolton-Hunter reagent. The antigen consists of a soluble preparation of the NIH-Z (HIV-2) strain of 1000X purified virus spiked with purified recombinant HIV-2 gp105. Radiolabeled proteins were immunoprecipitated by immune human sera, even at the early stages of seroconversion. This new assay provides a simple method for characterizing and titrating antibodies against HIV-2. The method is more sensitive, and is more efficient than Western blotting. The labeled viral proteins are well suited for biochemical studies.
Selvam
M P
MP
Laboratory of Immunochemistry, Food and Drug Administration, Bethesda, MD.
Mayner
R E
RE
Buck
S M
SM
Epstein
J S
JS
D015153:Blotting, Western; D006031:Glycosylation; D015483:HIV Antibodies / Q000097:blood*; D015498:HIV-2 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D015531:Radioimmunoprecipitation Assay; D012680:Sensitivity and Specificity; D013388:Succinimides
D015483:HIV Antibodies; D013388:Succinimides; C022581:Bolton-Hunter reagent