Ets oncogene-related gene Elg functions in Drosophila oogenesis.
Members of the ets gene family encode transcription factors that regulate the expression of a variety of cellular and viral genes including several protooncogenes. We have utilized Drosophila to elucidate the in vivo function of one family member. We show by complementation rescue and sequence analysis that the female sterile mutant tiny eggs (tne) is an allele of the Drosophila Ets-related gene Elg (also called D-elg). The mutation of a highly conserved tyrosine residue in the ETS DNA-binding domain of the Elg gene product demonstrates that normal gene function is required for proper follicle cell migration, chorion formation, and nurse cell-chromosome decondensation during Drosophila oogenesis.
Schulz
R A
RA
Department of Biochemistry and Molecular Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
The
S M
SM
Hogue
D A
DA
Galewsky
S
S
Guo
Q
Q
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins; D005260:Female; D005816:Genetic Complementation Test; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D005810:Multigene Family; D016296:Mutagenesis; D009866:Oogenesis / Q000235:genetics*; D010053:Ovary / Q000378:metabolism / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D015183:Restriction Mapping; D014157:Transcription Factors; D014170:Transformation, Genetic; D014443:Tyrosine
D017931:DNA Primers; D029721:Drosophila Proteins; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors; C070138:Ets97D protein, Drosophila; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases