The role of macrophages in antigen presentation and T cell tolerance.
Bone marrow derived cells (dendritic cells, macrophages and B cells) are involved in antigen presentation and T cell tolerance. However, the precise functions of each cell type remain unclear. To determine the role of macrophages we produced transgenic mice expressing I-E molecules only on macrophages, by introducing the hybrid gene containing the colony stimulating factor-1 (CSF-1) receptor promoter region and the structural gene encoding E alpha d into C57BL/6 mice. In these mice I-E restricted antigen presentation and T cell priming were impaired. With respect to T cell tolerance, I-E reactive T cells were anergized but not clonally deleted. These results clearly demonstrate that macrophages by themselves are defective in efficient I-E restricted antigen presentation, so that T cells exposed to antigens expressed on macrophages are led to anergy.
Miyazaki
T
T
Institute of Molecular Embryology and Genetics, Kumamoto University Medical School, Japan.
Suzuki
G
G
Yamamura
K
K
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology; D001483:Base Sequence; D005434:Flow Cytometry; D015870:Gene Expression; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007108:Immune Tolerance; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D012333:RNA, Messenger; D016186:Receptor, Macrophage Colony-Stimulating Factor