Reduced density of dopamine D2 receptors in the rat tail artery as a function of age.
The present study was designed to investigate the influence of aging on noradrenaline content and the density and pattern of prejunctional dopamine D2 receptors in the tail (ventral caudal) artery of male Sprague-Dawley rats. Tail artery is frequently used as a model for investigating mechanisms of sympathetic vascular control and contains prejunctional dopamine receptor belonging to the D2 subtype. Noradrenaline levels were reduced in rats of 12 months of age in comparison with 3-month-old animals. A further reduction in catecholamine concentration was found in 24-month-old rats. The density of prejunctional D2 receptors, which was measured in frozen sections of the tail artery by using both radioligand binding and autoradiographic techniques, was reduced by about 35% in 12-month rats in comparison with 3-month rats. A decrease by about 55% versus 3-month rats and by about 20% versus 12-month rats was observed in 24-month-old rats. Neither the pharmacological profile nor the anatomical localization of prejunctional D2 receptors was changed in the rat tail artery as a function of age. The parallel decrease in noradrenaline content and in the density of prejunctional dopamine D2 receptor sites in the tail artery of Sprague-Dawley rats of different ages probably accounts for the lack of an age-related change of prejunctional dopamine D2 receptor-linked responses reported in functional studies.
Mancini
M
M
Clinica Neurologica Università Tor Vergata & I.R.C.C.S., Clinica Santa Lucia, Roma, Italy.
Vega
J A
JA
Amenta
F
F
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001158:Arteries; D001345:Autoradiography; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000032:analysis*; D013623:Tail / Q000098:blood supply*
D017448:Receptors, Dopamine D2; D009638:Norepinephrine