Characterization of lethal alleles of D-elg, an ets proto-oncogene related gene with multiple functions in Drosophila development.
We have used genetic complementation rescue to identify two lethal alleles of D-elg, an ets proto-oncogene related gene of Drosophila. Animals that are hemizygous or trans-heterozygous for the alleles die as pharate adults, demonstrating normal gene function is required to complete Drosophila development. Females trans-heterozygous for the 1(3)902 lethal allele and the previously characterized tne female sterile allele produce embryos with abdominal segmentation defects. This finding implicates a role for the D-elg gene in anterior-posterior patterning. The cloning and sequencing of the lethal alleles identified molecular mutations that may result in ELG protein truncation, altered ELG protein interactions, or defective D-elg mRNA splicing.
Schulz
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Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Hogue
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The
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D000483:Alleles; D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000235:genetics*; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D017344:Genes, Insect / Q000235:genetics* / Q000502:physiology*; D005804:Genes, Lethal / Q000235:genetics* / Q000502:physiology*; D006579:Heterozygote; D009154:Mutation; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors
D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D014157:Transcription Factors