[Heterologous expression of functionally active human cytochrome P-450. I. Synthesis of cytochrome P-450IID6 by transformed Saccharomyces cerevisiae cells].
Functionally active human cytochrome P-450IID6 was expressed in Saccharomyces cerevisiae cells transformed with yeast expression vectors containing a fragment of the 2 mu-plasmid and cytochrome P-450 cDNA. Transcription was regulated by yeast acid phosphatase PHO5 promoter or chimeric GAL10-CYC1 promoter. Induced yeast cells synthesized cytochrome P-450 that was revealed by the optical absorption of the reduced CO form. Intact yeast cells were capable to metabolize the drug sparteine forming biotransformation products usually found in human. Expression of the human cytochromes P-450 seems to be a promising route to investigate new drugs and to screen genotoxic compounds.
Krynetskiĭ
E Iu
EIu
Kovaleva
I E
IE
El'darov
M A
MA
Luzikov
V N
VN
D000135:Acid Phosphatase / Q000235:genetics; D019389:Cytochrome P-450 CYP2D6; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D005822:Genetic Vectors; D006801:Humans; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D019389:Cytochrome P-450 CYP2D6; D000135:Acid Phosphatase
Geterologicheskaia ékspressiia funktsional'no aktivnykh tsitokhromov P-450 cheloveka. I. Sintez tsitokhroma P-450IID6 transformirovannymi kletkami Saccharomyces cerevisiae.