title: Testing of quantitative parameters in the 32P-postlabelling method.
abstract: The 32P-postlabelling technique involves many steps that need to be carefully controlled in order to obtain a reliable quantitative determination of DNA adducts. We have studied several of the parameters involved in the DNA digestion procedures as well as those concerned in the phosphorylation reaction. Since adducts behave in very different ways in the labelling reaction, an individual protocol has to be worked out for each particular type of adduct. Quantitation is usually possible only if a synthesized standard of the adduct under investigation is run in parallel to the DNA samples throughout the whole procedure.
authors.affiliation: Centre for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Hemminki
authors.forename: A
authors.initials: A
authors.lastname: Försti
authors.forename: M
authors.initials: M
authors.lastname: Löfgren
authors.forename: D
authors.initials: D
authors.lastname: Segerbäck
authors.forename: C
authors.initials: C
authors.lastname: Vaca
authors.forename: P
authors.initials: P
authors.lastname: Vodicka
mesh_terms: D000255:Adenosine Triphosphate; D002122:Calcium Chloride; D004247:DNA / Q000032:analysis*; D004249:DNA Damage; D003854:Deoxyribonucleotides / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes
chemical_list: D003854:Deoxyribonucleotides; D009838:Oligodeoxyribonucleotides; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D004247:DNA; D002122:Calcium Chloride