title: Crystallization and preliminary X-ray crystallographic analysis of chitinase from barley seeds.
abstract: Chitinase from barley seeds has been crystallized at room temperature using polyethylene glycol as precipitant. The crystal is monoclinic, belonging to the space group P2(1), with unit cell parameters of a = 69.43 A, b = 44.55 A, c = 81.41 A, and beta = 111.95 degrees. The asymmetric unit seems to contain two molecules of chitinase with a corresponding crystal volume per protein mass (VM) of 2.25 A3/Da and a solvent content of 45% by volume. The crystal diffracts to at least 2.0 A with X-rays from a rotating anode source and is very stable in the X-ray beam. X-ray data have been collected to better than 2.2 A Bragg spacing from a native crystal.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.
authors.forename: H K
authors.initials: HK
authors.lastname: Song
authors.forename: K Y
authors.initials: KY
authors.lastname: Hwang
authors.forename: K K
authors.initials: KK
authors.lastname: Kim
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
mesh_terms: D002688:Chitinases / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D001467:Hordeum / Q000201:enzymology*
chemical_list: D002688:Chitinases