A comparative study on the solid phase capture--platelet test, platelet adhesion immunofluorescence test and lymphocytotoxic test.
The sensitivity, specificity and reproducibility of the solid phase capture-platelet test (CPT) were compared with those of the platelet adhesion immunofluorescence test (PAIFT) and the lymphocytotoxic test (LCT). This study shows that CPT has a higher sensitivity than PAIFT (P < 0.001) and LCT (P < 0.01). The specificity was 86% lower than LCT (100%) (P < 0.01). Both the CPT test and PAIFT were comparable with regard to reproducibility (100%, 92%). The solid phase assay is easier to handle than PAIFT and LCT, allowing identification of HLA as well as of platelet specific antibodies. It is an efficient antibody screening test.
Jin
J
J
Department of Hematology, First Affiliated Hospital, Zhejiang Medical University, Hangzhou.
Lin
X J
XJ
Dorner
R
R
Sachs
V
V
D000918:Antibody Specificity; D001792:Blood Platelets / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D007518:Isoantibodies / Q000032:analysis*; D010973:Platelet Adhesiveness; D011696:Purpura, Thrombocytopenic / Q000276:immunology; D012680:Sensitivity and Specificity
D007518:Isoantibodies