title: Improved column-switching liquid chromatographic method for the determination of the enantiomers of mefloquine.
abstract: A liquid chromatographic method for the determination of the enantiomers of mefloquine has been improved. The chromatography involved two columns: an achiral cyanopropyl stationary phase for the quantification of (+/-)-mefloquine and a chiral naphthyl-urea stationary phase for the determination of the enantiomeric ratio. Compared with the previous method, which needed two detectors, this one used one detector-integrator to which the two columns are connected alternately by an automated column-switching system. The method is suitable for the quantification (0.05 microgram/ml) of mefloquine and the determination of enantiomeric ratios from 500-microliters plasma samples with ultraviolet detection.
authors.affiliation: Dosage de Médicaments, Service Pharmacie, Hôpital Pitié Salpêtriere, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Gimenez
authors.forename: C
authors.initials: C
authors.lastname: Dumartin
authors.forename: I W
authors.initials: IW
authors.lastname: Wainer
authors.forename: R
authors.initials: R
authors.lastname: Farinotti
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D015767:Mefloquine / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D015767:Mefloquine