32P-postlabelling analysis of alkylphosphotriesters in DNA.
We have developed a highly sensitive 32P-postlabelling assay for the detection of alkylphosphotriesters in DNA using selective enzymatic DNA hydrolysis, sample preparation by C18 Sep-Pak solid phase extraction and product analysis by HPLC. The assay provides information on the quantity of the alkylphosphotriesters and gives some clues with respect to their identity.
Saris
C P
CP
Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam.
Damman
S J
SJ
van den Ende
A M
AM
Westra
J G
JG
den Engelse
L
L
D000478:Alkylation; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004249:DNA Damage; D005069:Evaluation Studies as Topic; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D008722:Methods; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D051381:Rats
D010761:Phosphorus Radioisotopes; D004247:DNA