1 alpha,25-dihydroxy vitamin D3 modulation of HLA-DR mRNA induced by gamma-interferon in cultured epithelial tumor cell lines.
Using three cultured epithelial tumor cell lines, we investigated and analyzed the effects of gamma-interferon (gamma-IFN) and 1 alpha,25-dihydroxy vitamin D3 (1,25-(OH)2D3) on the levels of HLA-DR (alpha) mRNA and HLA-DR (beta) mRNA by Northern blot analysis. After treatment with gamma-IFN alone, the levels of the mRNA increased. Treatment with both gamma-IFN and 1,25-(OH)2D3 at the same time resulted in a significant decrease in the levels of mRNA in K-TL-1, IK-TL-2, and M-TL cells as compared to those induced by gamma-IFN alone.
Tone
T
T
Department of Dermatology, School of Medicine, Kitasato University, Kanagawa, Japan.
Eto
H
H
Katou
T
T
Otani
F
F
Nishiyama
S
S
D015152:Blotting, Northern; D002117:Calcitriol / Q000494:pharmacology*; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D018295:Neoplasms, Basal Cell / Q000235:genetics*; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured / Q000378:metabolism
D006684:HLA-DR Antigens; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma; D002117:Calcitriol