Localization of a Band 3-related protein in the mitochondria-rich cells of amphibian skin epithelium.
Based on immunoblotting procedure, the isolated epithelium of amphibian skin was found to contain a 180 kDa protein which cross-reacts with a polyclonal antiserum raised against human erythrocyte Band 3. Immunoperoxidase and immunofluorescence staining techniques indicated that the Band 3-related protein was localized in the mitochondria-rich cells (MRC) of this epithelium, with characteristic apical labelling pattern. Our findings show that the putative apical anion exchanger of the MRC is immunologically related to the band 3 multigenic family, which catalyzes Cl(-)-HCO3- transmembranous exchange. It thus suggests a molecular basis for the role played by these cells in the transepithelial Cl- pathway and acid-base regulation.
Devuyst
O
O
Department of Biology, Technion Institute, Haifa, Israel.
Rott
R
R
Denef
J F
JF
Crabbé
J
J
Katz
U
U
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis* / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D002025:Bufonidae; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D004912:Erythrocytes; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008928:Mitochondria / Q000648:ultrastructure*; D012867:Skin / Q000737:chemistry / Q000166:cytology*
D001457:Anion Exchange Protein 1, Erythrocyte; D001798:Blood Proteins; D007106:Immune Sera; D008565:Membrane Proteins