The changes of c-fos mRNA expression in the protein kinase C activated-osteoblastic MC3T3-E1 cells cultured in a low-calcium environment.
The effect of phorbol 12-myristate 13-acetate (TPA) on the c-fos mRNA expression in the osteoblastic MC3T3-E1 cells cultured in a low-calcium environment was examined. The c-fos mRNA expression in MC3T3-E1 cells with the treatment of TPA was enhanced under the low-calcium environment. This fact suggests that TPA induced-protein kinase C enhancement produced the increase of c-fos mRNA expression, and that protein kinase C may be intimately involved in c-fos mRNA expression.
Matsumoto
A
A
Department of Dental Pharmacology, School of Dentistry, Hokkaido University, Sapporo, Japan.
Deyama
Y
Y
Okitsu
M
M
Yoshimura
Y
Y
Suzuki
K
K
Hisada
Y
Y
D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D004789:Enzyme Activation; D010006:Osteoblasts / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003470:Culture Media; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium