O2 occlusion and cyanide induced immediate relaxation and contraction of murine skeletal muscle.
The acute changes of muscle tone and membrane current upon occlusion of oxygenation (O2 occlusion) were studied in vitro in mouse diaphragms. O2 occlusion immediately produced a contraction and a relaxation, respectively, in ryanodine- and high K(+)-contracted muscles while a biphasic change (an initial decrease then a late increase) of muscle tone was produced in muscles contracted with caffeine. The O2 occlusion effects were reversed after reoxygenation. CN- produced similar acute changes of muscle tone and abolished O2 occlusion effects. The O2 occlusion-induced relaxation in high K+ medium was converted into a contraction by 3,4-diaminopyridine and by low Cl- Tyrode's. O2 occlusion induced a small outward current and membrane hyperpolarization at a rate slower than the changes of muscle tone. Glybenclamide inhibited all of the changes induced by O2 occlusion. It is possible that the K+ and Cl- permeabilities of sarcoplasmic reticulum are highly sensitive to hypoxic challenge and related to the immediate changes of muscle tone after O2 occlusion.
Hong
S J
SJ
Department of Pharmacology, College of Medicine, National Taiwan University, Taipei, ROC.
Chang
C C
CC
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology*; D004558:Electric Stimulation; D005905:Glyburide / Q000494:pharmacology; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D011188:Potassium / Q000494:pharmacology; D012132:Respiratory Muscles / Q000187:drug effects* / Q000502:physiology*; D012433:Ryanodine / Q000494:pharmacology; D012964:Sodium / Q000502:physiology
D003486:Cyanides; D012433:Ryanodine; D002110:Caffeine; D012964:Sodium; D011188:Potassium; D005905:Glyburide