Misincorporation of ribonucleotides by DNA polymerase during in vitro DNA replication.
The fidelity of DNA replication with respect to misincorporation of ribonucleotides into DNA has been studied. Primed M13 DNA was replicated by E. coli DNA polymerase I in the presence of four dNTPs, a single rNTP and Mg2+ as a cofactor. All four rNTPs were incorporated into DNA in place of the corresponding dNTPs when rNTP/dNTP ratio was 1000:1. The frequency of rNTP misincorporation was C > G > A > U.
Ide
H
H
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Yagi
R
R
Yamaoka
T
T
Kimura
Y
Y
D017104:Bacteriophage M13 / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D004256:DNA Polymerase I / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D012265:Ribonucleotides / Q000378:metabolism*; D013698:Templates, Genetic
D004279:DNA, Viral; D012265:Ribonucleotides; D004256:DNA Polymerase I