title: Evaluation of the cloned enzyme donor immunoassay for measurement of phenytoin and phenobarbital in serum.
abstract: We report the evaluation of the cloned enzyme donor immunoassay (CEDIA) for the estimation of phenytoin and phenobarbital in serum. The assays were performed with a Hitachi 911 analyzer. Intra-assay coefficients of variation were from 1.5 to 4.4% for phenytoin and 1.6 to 5.5% for phenobarbital. Interassay coefficients of variation ranged from 1.8 to 5.3% for phenytoin and 2.9 to 4.8% for phenobarbital. Linearity was satisfactory, with a recovery of 103% at 11 mg/L and 110% at 22 mg/L phenytoin and 93% at 14 mg/L and 101% at 40 mg/L for phenobarbital. The detection limit was 1.2 mg/L for phenytoin and 0.6 mg/L for phenobarbital. Results of this assay correlated well with those of a conventional high-performance liquid chromatography (HPLC) method; r = 0.99 for phenytoin (n = 47) and r = 0.98 for phenobarbital (n = 48). The CEDIA was easy to handle and especially suitable for short turn-around time application.
authors.affiliation: Department of Clinical Chemistry, Psychiatric Hospital Veldwijk, Ermelo, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: van der Weide
authors.forename: H J
authors.initials: HJ
authors.lastname: Luiting
authors.forename: A H
authors.initials: AH
authors.lastname: Veefkind
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D010634:Phenobarbital / Q000097:blood* / Q000276:immunology; D010672:Phenytoin / Q000097:blood* / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010672:Phenytoin; D010634:Phenobarbital