Expression of glutathione S-transferase P-form in primary cultured rat liver parenchymal cells by coplanar polychlorinated biphenyl congeners is suppressed by protein kinase inhibitors and dexamethasone.
Glutathione S-transferase P-form (GST-P, EC 2.5.1.18) mRNA was expressed by epidermal growth factor as well as by 3,4,5,3',4'-penta-chlorinated biphenyl (PenCB) in primary cultured rat liver parenchymal cells. The expression of GST-P was suppressed by inhibitors of protein kinase C and dexamethasone, an antagonist of AP-1 transcription factor activity, whereas expression of cytochrome P450IA2 by PenCB was not affected by these reagents. The AP-1 related transcription factor may be essential for the expression of GST-P by PenCB as also may be a protein kinase C type enzyme.
Aoki
Y
Y
National Institute for Environmental Studies, Ibaraki, Japan.
Matsumoto
M
M
Suzuki
K T
KT
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000737:chemistry / Q000187:drug effects; D008099:Liver / Q000166:cytology; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000037:antagonists & inhibitors / Q000502:physiology; D051381:Rats
D016755:Proto-Oncogene Proteins c-jun; D004815:Epidermal Growth Factor; D003907:Dexamethasone; D011078:Polychlorinated Biphenyls; D005982:Glutathione Transferase; D011493:Protein Kinase C; C023035:3,4,5,3',4'-pentachlorobiphenyl