title: Expression of ras-P21 and ras gene alteration in pleomorphic adenomas.
abstract: The expression of ras-P21 protein and gene alteration of the K-ras gene were examined in 12 cases of pleomorphic adenoma of the salivary gland. The tumor cells in all 12 cases were strongly stained for ras-P21 protein by the indirect immunoperoxidase method, suggesting the enhanced expression of ras-P21 protein in pleomorphic adenomas. Analysis of the PCR products for the K-ras gene by temperature-gradient gel electrophoresis (TGGE) revealed that gene alterations such as point mutations occurred in 4 out of 12 pleomorphic adenoma tissues examined.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okutsu
authors.forename: A
authors.initials: A
authors.lastname: Takeda
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Nakajima
authors.forename: J
authors.initials: J
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Suda
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Usami
authors.forename: T
authors.initials: T
authors.lastname: Himiya
authors.forename: K
authors.initials: K
authors.lastname: Kusama
mesh_terms: D008949:Adenoma, Pleomorphic / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D015689:Oncogene Protein p21(ras) / Q000032:analysis / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000737:chemistry / Q000235:genetics*
chemical_list: D017931:DNA Primers; D015689:Oncogene Protein p21(ras)