Multifunctional effects of anticomplementary agent K-76 on carrageenan-induced colitis in the rabbit.
In this study the effect of K-76, a sesquiterpene compound with anticomplementary activity isolated from a fungus culture, on carrageenan-induced colitis was studied from biochemical, histological and immunohistopathological aspects. K-76 suppressed epithelial cell loss, crypt abscess formation, inflammatory cell infiltration, mucosal atrophy, and ulceration. Immunohistochemical examination of the colonic mucosa showed that the number of IgG- and IgM-positive plasma cells and the staining intensity for IgG and C3 were increased in carrageenan-induced colitis, but these changes were inhibited by K-76. Besides, serum mucoprotein concentrations and CH50 levels were lower in the animals treated with carrageenan alone. K-76 exerted multifunctional activity, although its mechanisms of action remain obscure.
Kitano
A
A
Department of Internal Medicine, Ashihara Hospital, Osaka, Japan.
Matsumoto
T
T
Nakamura
S
S
Oshitani
N
N
Obata
A
A
Okawa
K
K
Inoue
Y
Y
Kobayashi
K
K
Nakura
H
H
D000818:Animals; D002351:Carrageenan / Q000633:toxicity; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D003176:Complement C3 / Q000378:metabolism; D015941:Complement Hemolytic Activity Assay; D003169:Complement Inactivator Proteins / Q000493:pharmacokinetics / Q000494:pharmacology*; D004195:Disease Models, Animal; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009088:Mucoproteins / Q000097:blood; D011817:Rabbits; D012717:Sesquiterpenes / Q000097:blood / Q000494:pharmacology*
D003176:Complement C3; D003169:Complement Inactivator Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009088:Mucoproteins; D012717:Sesquiterpenes; C038797:K 76 carboxylic acid; D002351:Carrageenan