[Chlorhexidine digluconate/chlorocresol combination. In vitro study of interactions observed against potential cutaneous strains].
The interaction type of chlorhexidine digluconate/chlorocresol association (foaming solution DC 102/1, Laboratoires Pierre Fabre Médicament) have been studied according to the chess-board method, on microplate, by determining MBC (Minimal Bactericidal Concentrations) (or MFC (Minimal Fungicidal Concentrations)) adjusted to T 72-150 AFNOR norm (dilution-neutralization, contact 5 mn) and computation of FBC (or FFC) Index. Trials were carried out against 18 hospital strains (Staphylococcus epidermidis, Staphylococcus aureus, Propionibacterium acnes, Candida albicans) and against 4 strains of the AF-NOR norm. MBC of chlorhexidine, against Staphylococci were included between 6.25 and 25 micrograms/ml, and between 25 and 200 micrograms/ml against the others strains. MBC of chlorocresol were included between 800 and 3,200 micrograms/ml. Against the 22 tested strains, 18 synergistic interactions (FBC Index < or = 0.75) were observed, with 4 to 8 reducing factor for chlorhexidine, and > or = 2 for chlorocresol. No antagonism was noted (maximal FBC Index = 1).
Roques
C
C
Laboratoire de Bactériologie-Virologie et Microbiologie Industrielle, Faculté de Pharmacie, Toulouse, France.
Luc
J
J
Toselli
D
D
Michel
G
G
D002176:Candida albicans / Q000187:drug effects; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003408:Cresols / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004357:Drug Synergism; D016984:Enterococcus faecium / Q000187:drug effects*; D066298:In Vitro Techniques; D011425:Propionibacterium acnes / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013212:Staphylococcus epidermidis / Q000187:drug effects
D003408:Cresols; D004338:Drug Combinations; C006984:chlorocresol; C010882:chlorhexidine gluconate; D002710:Chlorhexidine
Association digluconate de chlorhexidine/chlorocrésol. Etude in vitro des interactions observées vis-à-vis de souches à potentiel cutané.