title: Nucleotide sequence of the Escherichia coli K-12 transketolase (tkt) gene.
abstract: The gene tkt for the enzyme transketolase (TK; EC 2.2.1.1) of Escherichia coli K-12 was subcloned and the complete DNA sequence was determined from both strands. An open reading frame with 1992 bp was detected that could encode a protein with a subunit mass of 72,143. The gene forms a monocistronic operon and is transcribed counterclockwise to the E. coli chromosome. From recombinant strains, the enzyme was purified and 39 N-terminal amino acid residues were determined. The DNA-derived protein sequence showed 49.5% identical amino acid residues with the transketolase of Rhodobacter sphaeroides, 46.8% identity with the enzyme from Saccharomyces cerevisiae, and 28.9% identity with the human transketolase.
authors.affiliation: Forschungszentrum Jülich GmbH, Institut für Biotechnologie 1, Jülich, Germany.
authors.forename: G A
authors.initials: GA
authors.lastname: Sprenger
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015183:Restriction Mapping; D016415:Sequence Alignment; D014174:Transketolase / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D014174:Transketolase