Expression and localization of intestinal 15 kDa protein in the rat.
Rat intestinal 15 kDa protein (I-15P) is highly homologous to porcine gastrotropin. We studied the occurrence, distribution and subcellular localization of I-15P in the entire rat body, using the immunocytochemistry to localize protein and in situ hybridization to localize mRNA. Both techniques demonstrated the expression of I-15P in the enterocytes of ileum, luteal cells of ovary and a subpopulation of steroid-endocrine cells of adrenal gland. Immuno-electron microscopy further demonstrated that I-15P is localized in both the cytoplasmic and nuclear matrix regions of these cells. The present results suggest roles of I-15P not only in the transport of bile salts but also in the metabolisms of certain steroid hormones.
Iseki
S
S
Department of Anatomy, School of Medicine, Kanazawa University, Japan.
Amano
O
O
Kanda
T
T
Fujii
H
H
Ono
T
T
D000311:Adrenal Glands / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis*; D015342:DNA Probes; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005260:Female; D005768:Gastrointestinal Hormones; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013552:Swine
D002352:Carrier Proteins; D015342:DNA Probes; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005768:Gastrointestinal Hormones; C057275:fatty acid-binding protein 6