beta-Amyloid precursor protein binds to the neurite-promoting IKVAV site of laminin.
We previously characterized a 110-kDa membrane-associated laminin-binding protein (LBP110) from brain which binds the laminin A chain -Ile-Lys-Val-Ala-Val-(IKVAV) site and increases in injury. Here we demonstrate that antisera directed against different epitopes of beta-amyloid precursor protein (APP) recognize LBP110 and that APP is recognized by LBP110 antiserum. APP specifically binds IKVAV and not another biologically active laminin-derived peptide containing the amino acid sequence -Tyr-Ile-Gly-Ser-Arg-. PC-12 cells transfected with antisense APP RNA produce less APP and LBP110, and they form fewer processes when cultured on either laminin or the IKVAV peptide. Thus, LBP110 is a member of the APP family and a function for APP in neurite outgrowth is now defined.
Kibbey
M C
MC
Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Jucker
M
M
Weeks
B S
BS
Neve
R L
RL
Van Nostrand
W E
WE
Kleinman
H K
HK
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D005822:Genetic Vectors; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology; D016501:Neurites / Q000502:physiology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D016372:RNA, Antisense; D014162:Transfection
D016564:Amyloid beta-Protein Precursor; D007797:Laminin; D009414:Nerve Growth Factors; D010446:Peptide Fragments; D016372:RNA, Antisense