Synthesis of full-length cDNA using DNA-capped mRNA.
We have developed a novel method for synthesizing a full-length cDNA based on the addition of DNA-RNA chimeric linker to the CAP site of mRNA. The DNA-capped mRNA was used as a template for cDNA synthesis with a dT-tailed vector primer. The model experiment using rabbit globin mRNA showed that more than 80% of the clones encoding alpha- and beta-globin contains a full-length cDNAs starting from the CAP site.
Sekine
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Kanagawa Academy of Science and Technology, Japan.
Kato
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D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D018076:DNA, Complementary / Q000138:chemical synthesis*; D005914:Globins / Q000235:genetics; D008969:Molecular Sequence Data; D012315:RNA Caps; D012333:RNA, Messenger / Q000737:chemistry*; D011817:Rabbits; D013698:Templates, Genetic
D018076:DNA, Complementary; D012315:RNA Caps; D012333:RNA, Messenger; D005914:Globins; D004247:DNA