Improved column-switching liquid chromatographic method for the determination of the enantiomers of mefloquine.
A liquid chromatographic method for the determination of the enantiomers of mefloquine has been improved. The chromatography involved two columns: an achiral cyanopropyl stationary phase for the quantification of (+/-)-mefloquine and a chiral naphthyl-urea stationary phase for the determination of the enantiomeric ratio. Compared with the previous method, which needed two detectors, this one used one detector-integrator to which the two columns are connected alternately by an automated column-switching system. The method is suitable for the quantification (0.05 microgram/ml) of mefloquine and the determination of enantiomeric ratios from 500-microliters plasma samples with ultraviolet detection.
Gimenez
F
F
Dosage de Médicaments, Service Pharmacie, Hôpital Pitié Salpêtriere, Paris, France.
Dumartin
C
C
Wainer
I W
IW
Farinotti
R
R
D002853:Chromatography, Liquid; D006801:Humans; D015767:Mefloquine / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D015767:Mefloquine