Base composition analysis of oligonucleotides containing apurinic sites.
Upon base composition analysis, oligonucleotides which are labeled at the 3'-terminus with fluorescein or biotin generate an additional, late eluting peak in the HPLC chromatogram. Investigation of this effect revealed the haptens acted as apurinic sites, and phosphodiesterase cleavage of the phosphate bond between the upstream nucleotide and apurinic site is inhibited. Extension of this work with a base-stable apurinic site inserted into all possible junctures of 5'-TGAC-3' tetramers showed this to be a general effect. As a consequence of this work, acid-catalyzed depurination resulting in apurinic sites can be monitored in oligonucleotide synthesis.
Morgan
R L
RL
Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064.
Celebuski
J E
JE
Fino
J R
JR
D001080:Apurinic Acid / Q000737:chemistry*; D001482:Base Composition; D001710:Biotin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019793:Fluorescein; D005452:Fluoresceins / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity
D005452:Fluoresceins; D009838:Oligodeoxyribonucleotides; D001080:Apurinic Acid; D001710:Biotin; D010727:Phosphoric Diester Hydrolases; D019793:Fluorescein