A lymphostromal molecule, thymic shared Ag-1, regulates early thymocyte development in fetal thymus organ culture.
Previously, we detailed the characterization of thymic shared Ag-1, a unique marker of immature thymocytes and isolated thymic stromal cells, defined by the mAb MTS 35. In this study, the functional relevance of this molecule to thymopoiesis was investigated by the addition of purified MTS 35 to fetal thymus organ culture. It down-regulated thymic shared Ag-1 expression and dramatically reduced thymocyte cell yield through inhibition of alpha beta-TcR+ T cell differentiation, post CD3-CD4-CD8- triple negative thymocytes. These effects were specific for the mAb MTS 35, because controls, which include both isotype-matched and other lymphostromal mAb, showed no similar effects. These results demonstrate that thymic shared Ag-1 is a functionally important marker of early thymocyte differentiation, particularly with regard to the alpha beta-TcR lineage.
Randle
E S
ES
Department of Pathology and Immunology, Monash University Medical School, Prahran, Australia.
Waanders
G A
GA
Masciantonio
M
M
Godfrey
D I
DI
Boyd
R L
RL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000502:physiology*; D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation; D005333:Fetus / Q000276:immunology*; D006410:Hematopoiesis; D051379:Mice; D008808:Mice, Inbred CBA; D009924:Organ Culture Techniques; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D017154:Stromal Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000502:physiology*; D013950:Thymus Gland / Q000196:embryology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D015703:Antigens, CD; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta