Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes.
The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections.
Tassanakajon
A
A
Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.
Boonsaeng
V
V
Wilairat
P
P
Panyim
S
S
D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D001483:Base Sequence; D016054:DNA, Protozoan / Q000302:isolation & purification; D005784:Gene Amplification; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D016054:DNA, Protozoan