title: Leucine aminopeptidase: an inducible component of the defense response in Lycopersicon esculentum (tomato).
abstract: A leucine aminopeptidase (EC 3.4.11.1) cDNA clone (DR57) that was induced in response to Pseudomonas syringae pv. tomato (P.s. tomato) infection was isolated using a subtractive hybridization-enriched cDNA probe. Genomic DNA blot analysis showed that the tomato genome had two leucine aminopeptidase genes. The levels of DR57 mRNAs after P.s. tomato infection and mechanical wounding were determined in two inbred tomato lines that exhibit susceptibility and resistance to P.s. tomato. DR57 mRNAs were detected 12 hours after infection and 4 hours after wounding. Furthermore, DR57 mRNAs were systemically induced in response to wounding. DR57 mRNAs were induced in leaves after Spodoptera littoralis feeding but were not detected in detached leaf controls. Possible roles for the DR57 leucine aminopeptidase in the defense reactions are discussed.
authors.affiliation: Department of Botany and Plant Sciences, University of California, Riverside 92521-0124.
authors.forename: V
authors.initials: V
authors.lastname: Pautot
authors.forename: F M
authors.initials: FM
authors.lastname: Holzer
authors.forename: B
authors.initials: B
authors.lastname: Reisch
authors.forename: L L
authors.initials: LL
authors.lastname: Walling
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D015342:DNA Probes; D004790:Enzyme Induction; D015723:Gene Library; D007931:Leucyl Aminopeptidase / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000201:enzymology; D014675:Vegetables / Q000201:enzymology* / Q000502:physiology
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D007931:Leucyl Aminopeptidase