title: Cytotoxicity of retrofill materials.
abstract: Dentin bonding agents reduce microleakage and enhance marginal adaption of composite resin restorations. These characteristics are advantages for their use as an endodontic retrofilling material. Because these materials will be in direct contact with vital tissues, their cytotoxic potential must be evaluated before clinical use. It was the purpose of this study to evaluate the cell cytotoxicity of amalgam, Caulk Universal Bond, Gluma, 35% HEMA, Morita Clearfil, Scotchbond 2, Super EBA, Tenure, and Tenure 5-4. VERO cells were grown in RPMI-1640 medium and cell monolayers were prepared by incubating 15 ml of the cell suspension in 60-mm culture dishes at 37 degrees C in 5% CO2. Twelve milliliters of a medium-agarose mixture containing 1% neutral red vital stain were overlayed onto the cell layer and allowed to solidify. The materials were directly exposed to the agarose overlays by inverting 6.0-mm diameter polypropylene capsules containing the cured and liquid sample materials either immediately (0 time) or after placement in phosphate-buffered saline with 1% gentamicin for 7, 15, or 30 days. Cytotoxicity was determined by measuring the zone of killed cells around the sample 24 h after placement on the agarose. Cytotoxicity was determined by measuring the zones of cell inhibition at 24 h and at 7, 15, and 30 days. Initially, all of the materials were found to be cytotoxic, except amalgam and the Tenure components. The dentin bonding primers showed a mean zone of inhibition of 13.2 mm and the cleansers a 40.0-mm zone. Amalgam demonstrated increasing cytotoxicity: 0.0 mm at 24 h to 12.0 mm at 30 days.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Endodontics, Baltimore College of Dental Surgery, Dental School, University of Maryland at Baltimore 21201.
authors.forename: G R
authors.initials: GR
authors.lastname: Bruce
authors.forename: N J
authors.initials: NJ
authors.lastname: McDonald
authors.forename: R J
authors.initials: RJ
authors.lastname: Sydiskis
mesh_terms: D000134:Acid Etching, Dental; D000704:Analysis of Variance; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity; D003188:Composite Resins / Q000633:toxicity; D003722:Dental Alloys / Q000633:toxicity; D003723:Dental Amalgam / Q000633:toxicity; D003736:Dental Cavity Lining; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D005976:Glutaral / Q000633:toxicity; D008422:Materials Testing; D008689:Methacrylates / Q000633:toxicity; D017942:Nitric Acid / Q000633:toxicity; D010070:Oxalates / Q000633:toxicity; D011109:Polymethacrylic Acids / Q000633:toxicity; D019279:Resin Cements; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013997:Time Factors; D014709:Vero Cells
chemical_list: C084075:Caulk Universal Bond; D003188:Composite Resins; D003722:Dental Alloys; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D010070:Oxalates; D011109:Polymethacrylic Acids; D019279:Resin Cements; D012387:Root Canal Filling Materials; C084076:Tenure 5-4; C061140:Tenure Dentin Conditioner; C067788:scotchbond 2; C074811:Tenure adhesive; D017942:Nitric Acid; D017438:Bisphenol A-Glycidyl Methacrylate; C031360:Sybraloy; C005044:hydroxyethyl methacrylate; D003723:Dental Amalgam; C084074:Super EBA; C044372:Gluma; D005976:Glutaral