title: A high-resolution cytogenetic map of human chromosome 12: localization of 195 new cosmid markers by direct R-banding fluorescence in situ hybridization.
abstract: We have constructed a high-resolution cytogenetic map of human chromosome 12 with 195 newly isolated cosmids by direct R-banding fluorescence in situ hybridization. The fluorescent signals of 195 clones were evenly distributed throughout chromosome 12, but sublocalized preferentially to R-positive bands. This high-resolution cytogenetic map with an average map distance of 0.73 Mb on bands can, in conjunction with a genetic linkage map, facilitate the analysis of chromosomal and molecular aberrations in genetic diseases and cancers. Moreover, the cytogenetic mapping data provide starting points for establishing contig maps with cosmid clones and yeast artificial chromosomes.
authors.affiliation: Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Koyama
authors.forename: A
authors.initials: A
authors.lastname: Hitomi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D003360:Cosmids / Q000235:genetics*; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice
chemical_list: D005819:Genetic Markers; D004247:DNA