An ionizing radiation-sensitive mutant of CHO cells: irs-20. II. Dose-rate effects and cellular recovery processes.
An ionizing radiation-sensitive mutant of CHO cells, irs-20, irradiated in plateau-phase cultures showed a complete lack of a dose-rate effect, i.e., there was no reduction in cell killing per unit dose as the dose rate was lowered from 0.75 Gy/min to 0.06 Gy/h. No measurable repair of potentially lethal damage or sublethal damage was evident for irs-20 cells in the G1 phase of the cell cycle. During S phase, irs-20 cells showed repair of sublethal damage similar to that for the wild-type cells.
Stackhouse
M A
MA
Department of Radiological Health Sciences, Colorado State University, Fort Collins 80523.
Bedford
J S
JS
D000818:Animals; D016466:CHO Cells / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004307:Dose-Response Relationship, Radiation; D009154:Mutation; D011836:Radiation Tolerance; D011839:Radiation, Ionizing