title: A strain-specific cyclin homolog in the fungal phytopathogen Colletotrichum gloeosporioides.
abstract: The fungus, Colletotrichum gloeosporioides, which infects the tropical pasture legume, Stylosanthes guianensis, contains highly variable mini-chromosomes. The transcription of strain-specific genomic DNA clones previously isolated from one variable mini-chromosome was investigated by using these clones to screen a cDNA library prepared from the fungus grown in liquid medium. A cDNA clone was obtained with one of the genomic clones and was sequenced. A single long open reading frame of 259 amino acids (aa) was detected with significant homology to cyclin proteins in other organisms. Northern blot analysis indicated that the cDNA corresponded to a low-abundance mRNA (approximately 0.001% of poly(A)+RNA). Southern blot analysis indicated that genes encoding this mRNA were discontinuously distributed in this fungal species, indicating it encodes a dispensable function. This result suggests that natural populations of fungi may have variable complements of cyclin-encoding genes.
authors.affiliation: Cooperative Research Centre for Tropical Plant Pathology, University of Queensland, Brisbane, Australia.
authors.forename: A M
authors.initials: AM
authors.lastname: Masel
authors.forename: N
authors.initials: N
authors.lastname: Struijk
authors.forename: C L
authors.initials: CL
authors.lastname: McIntyre
authors.forename: J A
authors.initials: JA
authors.lastname: Irwin
authors.forename: J M
authors.initials: JM
authors.lastname: Manners
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D016213:Cyclins / Q000235:genetics*; D004271:DNA, Fungal; D005656:Fungal Proteins; D016681:Genome, Fungal; D003904:Mitosporic Fungi / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: C084272:CYC1 protein, Colletotrichum gloeosporioides; D018797:Cell Cycle Proteins; D016213:Cyclins; D004271:DNA, Fungal; D005656:Fungal Proteins