Receptor binding profile of neuropeptide gamma and its fragments: comparison with the nonmammalian peptides carassin and ranakinin at three mammalian tachykinin receptors.
The tachykinin binding site preferences of neuropeptide gamma (NP gamma), its C-terminal fragments AcNP gamma(3-21), AcNP gamma(5-21), AcNP gamma(7-21), and AcNP gamma(9-21), other mammalian tachykinins, and the nonmammalian tachykinins ranakinin and carassin were examined in membrane binding competition studies. [125I]-Bolton-Hunter [Sar9,Met(O2)11]SP (BHSarSP), [125I]-neurokinin A (INKA) and [125I]-Bolton-Hunter scyliorhinin II (BHScyII) were used to investigate NK-1, NK-2, and NK-3 sites, in rat submandibular gland, gastric fundus, and brain, respectively. Elongation of the neurokinin A molecule does not appear to influence binding to rat tachykinin NK-1 and NK-2 binding sites. Ranakinin has affinity for the NK-1 and NK-2 site similar to that of substance P and neurokinin A, respectively, but has low affinity for the NK-3 site. Despite its structural similarities to neuropeptide gamma, carassin has only moderate affinity for rat tachykinin binding sites. Possession of an acidic residue at position 4 appears critical for binding to rat NK-2 sites.
Badgery-Parker
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School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia.
Lovas
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Conlon
J M
JM
Burcher
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E
D000595:Amino Acid Sequence; D000818:Animals; D002024:Bufo marinus; D005260:Female; D006054:Goldfish; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011895:Rana ridibunda; D051381:Rats; D017208:Rats, Wistar; D018039:Receptors, Tachykinin / Q000378:metabolism*; D015320:Tachykinins / Q000378:metabolism*
D009479:Neuropeptides; D009842:Oligopeptides; D010446:Peptide Fragments; D018039:Receptors, Tachykinin; D015320:Tachykinins; C059905:tachykinin neuropeptide gamma; C067794:carassin; C071642:ranakinin