Guinea-pig neutrophil cationic peptides are encoded by at least three kinds of mRNA transcripts.
1. cDNA clones encoding antimicrobial guinea-pig neutrophil cationic peptides, GNCP-1 and GNCP-2 were isolated from a bone marrow cell cDNA library. 2. Three kinds of cDNAs (GNCP-1A, GNCP-1B and GNCP-2 cDNAs) were obtained, and the analysis of these cDNAs indicated that GNCPs were produced as precursor proteins comprising 93 amino acid residues, which were composed of signal sequences (N-terminal 19 residues), pro-peptide sequences (43 residues) and mature GNCP sequences (31 residues). 3. The deduced amino acid sequences showed that GNCP-1A and GNCP-1B differed by only one amino acid substitution in the pro-peptide region, and GNCP-1B and GNCP-2 differed by only one amino acid substitution in the mature peptide region. The nucleotide sequences of these cDNAs were highly homologous (99%). 4. Together these observations indicate that GNCPs are encoded by at least three kinds of mRNA transcripts which are likely derived from the similar genes.
Nagaoka
I
I
Department of Biochemistry, Juntendo University, School of Medicine, Tokyo, Japan.
Nonoguchi
A
A
Yamashita
T
T
D000483:Alleles; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D005815:Genetic Code; D006168:Guinea Pigs / Q000097:blood*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D012333:RNA, Messenger