Crystallization and preliminary X-ray diffraction analysis of the complex of human placental 17 beta-hydroxysteroid dehydrogenase with NADP+.
Single crystals of human placental 17 beta-hydroxysteroid dehydrogenase, an enzyme that plays an important role in the interconversion of estrogens, were obtained as the NADP+ complex. These are the first crystals suitable for complete X-ray structure analysis ever reported for a steroid-converting enzyme from a human source. The crystals were grown by vapor diffusion at pH 7.5 with polyethyleneglycol (4000) as the precipitating agent. They have a monoclinic space group C2 and unit cell parameters are a = 123.03 A, b = 45.03 A, c = 61.29 A, and beta = 99.1 degrees. A complete set of diffraction data to 2.9 A has been collected on native crystals.
Zhu
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DW
CHUL Research Center, Quebec, Canada.
Lee
X
X
Breton
R
R
Ghosh
D
D
Pangborn
W
W
Daux
W L
WL
Lin
S X
SX
D015067:17-Hydroxysteroid Dehydrogenases / Q000648:ultrastructure*; D018360:Crystallography, X-Ray; D006801:Humans; D066298:In Vitro Techniques; D009249:NADP / Q000737:chemistry; D010920:Placenta / Q000201:enzymology
D009249:NADP; D015067:17-Hydroxysteroid Dehydrogenases