A positive correlation between the precursor frequency of cytotoxic lymphocytes to autologous Epstein-Barr virus-transformed B cells and antibody titer level against Epstein-Barr virus-associated nuclear antigen in healthy seropositive individuals.
A limiting dilution analysis was established to determine the precursor frequency (PF) of cytotoxic lymphocytes against autologous B cells transformed with the Epstein-Barr virus (EBV). This method was found to detect mainly self-restricted T-cell activity and little non-self-restricted cytotoxicity. The mean PF in 21 healthy EBV-seropositive persons was 1.4 x 10(-3) (range: 0.03 x 10(-3) to 8.7 x 10(-3)) for peripheral blood mononuclear cells, whereas 4 samples of mononuclear cells obtained from umbilical cord blood had PFs below 0.007 x 10(-3). A positive correlation was observed between the PF and serum antibody titers against EBV-associated nuclear antigen among the seropositive persons.
Kusunoki
Y
Y
Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.
Huang
H
H
Fukuda
Y
Y
Ozaki
K
K
Saito
M
M
Hirai
Y
Y
Akiyama
M
M
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000032:analysis*; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D007694:Killer Cells, Natural / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology; D013234:Stem Cells / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014412:Tumor Virus Infections / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D001324:Autoantigens; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens