Enzymology and gene technology of Streptomyces nucleotide 3'-pyrophosphokinase-2',3'-cyclic monophosphokinase (PPKase).
Streptomyces extracellular nucleotide 3'-pyrophosphokinase-2',3'-cyclic monophosphokinase transfers 5'-beta,gamma-pyrophosphate group from ATP, some ATP derivatives and dATP to a variety of nucleot(s)ides at the 3'-OH site and synthesizes the respective 3'-pyrophosphoryl nucleotides. The enzyme also utilizes A-5'-pn (n = 3-5)-5'-N, transferring their adenosine 5'-pyrophosphoryl group and concomitantly eliminating the AMP moiety therefrom, leading to the synthesis of 2',3'-cyclic monophosphoryl nucleotides. The enzyme gene and its neighbouring up- and downstream sequences were analyzed. Streptomyces and enteric bacteria E. coli and Klebsiella pneumoniae transformants were constructed by incorporation of the pyrophosphokinase gene-recombined expression plasmids. Various effects of the treatments--intracellular synthesis of 3'-pyrophosphoryl nucleotides, retarded cellular growth, stimulated N2 fixation by K. pneumoniae and so on--were seen.
Ezaki
S
S
Graduate School of Genetic Resources Technology, Kyusyu University, Fukuoka, Japan.
Muta
S
S
Kuhara
S
S
Mukai
J
J
D000227:Adenine Nucleotides / Q000378:metabolism; D003001:Cloning, Molecular; D017854:Diphosphotransferases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000235:genetics; D017014:Methanobacterium / Q000201:enzymology; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology*; D014170:Transformation, Genetic
D000227:Adenine Nucleotides; D017854:Diphosphotransferases; C084412:nucleotide 3'-pyrophosphokinase-2',3'-cyclic monophosphokinase