Development of a shape vector that identifies critical forms assumed by human polymorphonuclear neutrophils during chemotaxis.
Human polymorphonuclear neutrophils undergo characteristic shape changes that are critical to their ability to move and ingest their targets. We present here the construction of a simple shape vector, calculated from the coordinates of the cell perimeter, that can identify critical forms that a neutrophil assumes during the course of ameboid movement. The vector can be used to find neutrophils of a specific shape from the image analyzer data produced during a typical neutrophil tracking experiment.
Hartman
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RS
Division of Pediatric Hematology/Oncology, Childrens Hospital of Los Angeles, University of Southern California School of Medicine 90027.
Lau
K
K
Chou
W
W
Coates
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TD
D000546:Amanitins; D048429:Cell Size; D002634:Chemotaxis, Leukocyte; D005456:Fluorescent Dyes; D006801:Humans; D008433:Mathematics; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D009504:Neutrophils / Q000502:physiology / Q000648:ultrastructure*; D014966:Xanthenes
D000546:Amanitins; D005456:Fluorescent Dyes; D014966:Xanthenes; C028877:7-nitrobenz-2-oxa-1,3-diazole-phallacidin; C034657:Texas red