Trimeric mutant bacteriorhodopsin, D85N, shows a monophasic CD spectrum.
The structure of mutant bacteriorhodopsin (bR), D85N, was examined by CD and X-ray diffraction at pH 7. The absorption maximum of D85N at pH 7 is located at 605 nm, which is similar to the acid-blue form of wild-type bR. D85N shows a monophasic CD band, the maximum of which is at 575 nm, although the crystalline arrangement and the trimeric structure is maintained. The acid-blue form of wild-type bR shows a biphasic CD despite the similarity in absorption spectra.
Kataoka
M
M
Department of Biology, Faculty of Science, Osaka University, Toyonaka, Japan.
Mihara
K
K
Kamikubo
H
H
Needleman
R
R
Lanyi
J K
JK
Tokunaga
F
F
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D014961:X-Ray Diffraction
D001436:Bacteriorhodopsins