Oesophagostomum radiatum: the effect of different adjuvants on vaccination with a partially purified protective antigen.
The components of a soluble extract of adult Oesophagostomum radiatum were separated into four fractions by gel filtration chromatography on a Superose-12 column. The second fraction had a molecular weight range of approximately 60,000-65,000 as judged by SDS gel electrophoresis. Vaccination of calves with this fraction significantly (P < 0.05) reduced faecal egg output after challenge infection. In addition, the packed cell volume of vaccinated calves was higher than that of control calves (P < 0.05). The effect of three adjuvants on the vaccine activity was compared. Fraction 2 presented in dextran sulfate reduced the faecal egg count by 93.6% (P < 0.05), Fraction 2 in Freund's complete adjuvant reduced the faecal egg count by 27% (P = N.S.). Quil A was ineffective as an adjuvant.
East
I J
IJ
CSIRO, Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Qld., Australia.
Fitzgerald
C J
CJ
Berrie
D A
DA
D000276:Adjuvants, Immunologic; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D002850:Chromatography, Gel / Q000662:veterinary; D016264:Dextran Sulfate / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D005620:Freund's Adjuvant / Q000276:immunology; D009814:Oesophagostomiasis / Q000517:prevention & control / Q000662:veterinary*; D009815:Oesophagostomum / Q000276:immunology*; D010270:Parasite Egg Count / Q000662:veterinary; D062605:Quillaja Saponins; D012503:Saponins / Q000276:immunology; D014611:Vaccination / Q000662:veterinary
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D062605:Quillaja Saponins; D012503:Saponins; C046386:Quil A; D005620:Freund's Adjuvant; D016264:Dextran Sulfate