title: Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb.
abstract: The purpose of this investigation was to evaluate insulin-stimulated skeletal muscle glucose uptake in male Fischer 344 rats, age 6, 12, and 27 mo, fed either a sucrose (S: 66.6% sucrose, 17.6% protein, 6.4% fat) or sucrose-free (SF: 66.6% starch, 17.6% protein, 6.4% fat) diet for 3 mo. Skeletal muscle glucose uptake (Rg) in perfused hindlimbs was estimated from the uptake and subsequent phosphorylation of radiolabeled 2-deoxyglucose (2DG) in the gastrocnemius (GN), extensor digitorum longus (EDL), and soleus (SOL) muscles. Rat hindlimbs were perfused at a rate of 10 ml.min-1 with a modified Krebs Henseleit buffer containing bovine red blood cells (hematocrit: 40%) and 5.85 mmole.L-1 glucose along with 358 pmoles.L-1 followed by 3580 pmoles.L-1 insulin. There was no effect of diet on plasma glucose levels measured at weeks 1, 7, and 11 of the dietary period. A significant effect of age on estimated glucose uptake in the GN was demonstrated due primarily to greater uptake in the 27-mo compared to the 6-mo-old animals. This significant effect of age was not evident in the EDL or SOL, nor were there significant effects of diet in any muscle. These data suggest that insulin-stimulated glucose uptake in perfused hindlimbs is not attenuated with senescence or with the feeding of a sucrose diet for 3 months.
authors.affiliation: Department of Nutrition, University of California, Davis.
authors.forename: K C
authors.initials: KC
authors.lastname: Eiffert
authors.forename: J S
authors.initials: JS
authors.lastname: Stern
authors.forename: B A
authors.initials: BA
authors.lastname: Horwitz
authors.forename: L M
authors.initials: LM
authors.lastname: Larkin
authors.forename: R B
authors.initials: RB
authors.lastname: McDonald
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001834:Body Water / Q000378:metabolism; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D005947:Glucose / Q000378:metabolism; D006614:Hindlimb; D007328:Insulin / Q000494:pharmacology; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013395:Sucrose / Q000008:administration & dosage*
chemical_list: D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007328:Insulin; D010725:Phosphocreatine; D013395:Sucrose; D000255:Adenosine Triphosphate; D003847:Deoxyglucose; D005947:Glucose