title: Identification of the gene encoding the major cellobiohydrolase of the white rot fungus Phanerochaete chrysosporium.
abstract: Previous studies have shown that the cellobiohydrolases of the white rot basidiomycete Phanerochaete chrysosporium are encoded by a family of structurally related genes. In this investigation, we identified and sequenced the most highly transcribed gene, cbh1-4. Evidence suggests that in this fungus the dominant isozyme, CBH1, is encoded by chb1-4.
authors.affiliation: Institute for Microbial and Biochemical Technology, U.S. Department of Agriculture, Madison, Wisconsin 53705-2398.
authors.forename: A
authors.initials: A
authors.lastname: Vanden Wymelenberg
authors.forename: S
authors.initials: S
authors.lastname: Covert
authors.forename: D
authors.initials: D
authors.lastname: Cullen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics*; D043366:Cellulose 1,4-beta-Cellobiosidase; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics*; D007526:Isoelectric Point; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D007527:Isoenzymes; D006026:Glycoside Hydrolases; D043366:Cellulose 1,4-beta-Cellobiosidase