A simplified method for phenotyping alpha-2-HS-glycoprotein.
Alpha-2-HS-glycoprotein is considered a reliable genetic marker for paternity testing and forensic investigation. We describe a simple, semiautomated method for phenotyping this protein in serum samples, bloodstains and dental pulp using isoelectric focusing methods followed by immunofixation techniques. This method produces clear results even with very old biological evidence.
López-Abadía
I
I
Department of Legal Medicine, University Complutense, Madrid, Spain.
Ruiz de la Cuesta
J M
JM
D001798:Blood Proteins / Q000032:analysis / Q000235:genetics*; D001801:Blood Stains; D003782:Dental Pulp / Q000737:chemistry; D005819:Genetic Markers; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D010334:Paternity; D010641:Phenotype; D060749:alpha-2-HS-Glycoprotein
C556354:AHSG protein, human; D001798:Blood Proteins; D005819:Genetic Markers; D060749:alpha-2-HS-Glycoprotein