Detection and identification of equine herpesvirus-1 and -4 by polymerase chain reaction.
A rapid method for detection and identification of equine herpesvirus-1 and -4 (EHV-1 and EHV-4) was developed using polymerase chain reaction (PCR). Primers for PCR were designed from aligned nucleotide sequences of glycoprotein B genes of EHV-1 and EHV-4 to amplify specific regions for EHV-1 or EHV-4 or a common region of both viruses. By using type specific primer mixture, amplified fragments were identified as EHV-1 or EHV-4 in a one-step reaction. We have applied this technique on specimens from aborted fetuses. The samples contained only EHV-1 and there was complete accordance between the results of PCR and virus isolation. Our PCR system could differentiate the two virus types rapidly in a one-step reaction.
Kirisawa
R
R
Department of Veterinary Microbiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Endo
A
A
Iwai
H
H
Kawakami
Y
Y
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000662:veterinary; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D018140:Varicellovirus / Q000235:genetics*
D017931:DNA Primers; D004279:DNA, Viral; D015345:Oligonucleotide Probes