title: Catabolite inactivation of the yeast maltose transporter is due to proteolysis.
abstract: The maltose transport capacity of fermenting Saccharomyces cerevisiae rapidly decreases when protein synthesis is impaired. Using polyclonal antibodies against a recombinant maltose transporter-protein we measured the cellular content of the transporter along this inactivation process. Loss of transport capacity was paralleled by a decrease of cross-reacting material which suggests degradation of the transporter. We also show that in ammonium-starved cells the half-life of the maltose transporter is 1.3 h during catabolism of glucose and > 15 h during catabolism of ethanol.
authors.affiliation: Instituto de Investigaciones Biomédicas de CSIC, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Lucero
authors.forename: M
authors.initials: M
authors.lastname: Herweijer
authors.forename: R
authors.initials: R
authors.lastname: Lagunas
mesh_terms: D000906:Antibodies / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D008320:Maltose / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C067575:maltose transport system, S cerevisiae; D008320:Maltose