CoA and fatty acyl-CoA derivatives mobilize calcium from a liver reticular pool.
The effect of CoA and fatty acyl-CoA esters on Ca2+ fluxes has been studied in isolated liver microsomes and in digitonin-permeabilized hepatocytes. When microsomes were loaded with increasing concentrations of Ca2+ (6-29 nmol/mg of protein), the extent to which CoA and palmitoyl-CoA released Ca2+ increased. At 23 nmol of Ca2+/mg of protein, half-maximal [CoA] and [palmitoyl-CoA] were 35 and 50 microM respectively. Under conditions of minimal Ca2+ loading, net release of Ca2+ was absent, but Ca2+ translocation from a CoA-sensitive to a CoA-insensitive pool took place. The effect of CoA required the presence of fatty acids, probably to form fatty acyl esters. In permeabilized hepatocytes, the pool(s) mobilized by CoA (or by palmitoyl-CoA) appeared to be different from that mobilized by Ins(1,4,5)P3.
Fulceri
R
R
Instituto di Patologia Generale, University of Siena, Italy.
Gamberucci
A
A
Bellomo
G
G
Giunti
R
R
Benedetti
A
A
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003065:Coenzyme A / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004951:Esterification; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D051381:Rats
D000214:Acyl Coenzyme A; D010171:Palmitoyl Coenzyme A; D015544:Inositol 1,4,5-Trisphosphate; D000255:Adenosine Triphosphate; D003065:Coenzyme A; D002118:Calcium