title: Does phosphoglucoisomerase display anomeric specificity or selectivity towards alpha-D-glucose 6-phosphate? An assessment by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r.
abstract: The study by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r. of hexose 6-phosphates interconversion in the reaction catalysed by yeast phosphoglucoisomerase reveals that the enzyme displays anomeric selectivity, rather than specificity, towards alpha-D-glucose 6-phosphate. Indeed, beta-D-glucose 6-phosphate participates for about 20% to the total and direct conversion of the aldohexose into oxohexose ester.
authors.affiliation: High Resolution NMR Centre, Faculty of Engineering, Free University of Brussels (VUB), Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Kayser
authors.forename: M
authors.initials: M
authors.lastname: Biesemans
authors.forename: R
authors.initials: R
authors.lastname: Willem
authors.forename: F
authors.initials: F
authors.lastname: Malaisse-Lagae
authors.forename: W J
authors.initials: WJ
authors.lastname: Malaisse
mesh_terms: D019298:Glucose-6-Phosphate; D005956:Glucose-6-Phosphate Isomerase / Q000737:chemistry / Q000378:metabolism*; D005958:Glucosephosphates / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D010759:Phosphorus Isotopes; D013379:Substrate Specificity
chemical_list: D005958:Glucosephosphates; D010759:Phosphorus Isotopes; D019298:Glucose-6-Phosphate; D005956:Glucose-6-Phosphate Isomerase