Lectins: reliable differentiation markers in human oligodendrogliomas.
Ninety tumours classified as well-differentiated oligodendrogliomas (43 cases) and anaplastic oligodendrogliomas (47 cases) were studied with glial fibrillary acidic protein (GFAP) and bound with different lectins including: peanut agglutinin (PNA), concanavalin A (Con A), wheat germ agglutinin (WGA) and Ricinus communis agglutinin (RCA-1). PNA has shown high affinity to cellular membranes of well-differentiated oligodendrogliomas. The affinity decreases with the cellular differentiation to astrocytic lines and is lost in anaplastic oligodendrogliomas. Con A labelling is restricted to anaplastic oligodendroglioma cells and to reactive astrocytes showing a predominant somatic (cytoplasmatic) pattern of staining. Our findings showed that a combined search with GFAP and lectins may allow an accurate grading of oligodendrogliomas.
Figols
J
J
Institute of Neuropathology, Free University of Berlin.
Cervós-Navarro
J
J
Cruz-Sánchez
F F
FF
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003208:Concanavalin A; D005260:Female; D006801:Humans; D037102:Lectins; D008297:Male; D008875:Middle Aged; D009837:Oligodendroglioma / Q000473:pathology*; D019887:Peanut Agglutinin; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins
D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A