title: Effect of leucocyte products on platelet thrombus formation, coagulation and spontaneous thrombolysis, as measured from native human blood, in vitro.
abstract: Contribution of leucocytes to formation and lysis of arterial (platelet) thrombi was investigated. Secretory products of polymorphonuclear leucocytes (PMNs) during phagocytosis and cell lysates were prepared from eight volunteers. Platelet-rich thrombi were formed in flowing native human blood either by shear-stress or by collagen fibre, by haemostatometry. Tested in eight volunteers, PMN products significantly enhanced both thrombotic reactions and induced lysis of these thrombi. A specific inhibitor of leucocyte proteases (eglin c) inhibited platelet reaction to shear-stress and collagen and spontaneous thrombolysis. Our findings provide further evidence for the prothrombotic and potent thrombolytic effect of leucocytes associated with an arterial thrombus.
authors.affiliation: Laboratory of Physiology, Faculty of Nutrition, Kobe-Gakuin University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Yamamoto
authors.forename: I
authors.initials: I
authors.lastname: Ishii
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: C D
authors.initials: CD
authors.lastname: Ridler
authors.forename: P
authors.initials: P
authors.lastname: Gorog
authors.forename: I B
authors.initials: IB
authors.lastname: Kovacs
mesh_terms: D001685:Biological Factors / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D003094:Collagen; D005342:Fibrinolysis / Q000187:drug effects*; D006487:Hemostasis / Q000187:drug effects; D006801:Humans; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D011506:Proteins; D015843:Serpins / Q000302:isolation & purification / Q000494:pharmacology; D013927:Thrombosis / Q000503:physiopathology*
chemical_list: D001685:Biological Factors; D011506:Proteins; D015843:Serpins; C028656:eglin proteinase inhibitors; D003094:Collagen