Catabolite inactivation of the yeast maltose transporter is due to proteolysis.
The maltose transport capacity of fermenting Saccharomyces cerevisiae rapidly decreases when protein synthesis is impaired. Using polyclonal antibodies against a recombinant maltose transporter-protein we measured the cellular content of the transporter along this inactivation process. Loss of transport capacity was paralleled by a decrease of cross-reacting material which suggests degradation of the transporter. We also show that in ammonium-starved cells the half-life of the maltose transporter is 1.3 h during catabolism of glucose and > 15 h during catabolism of ethanol.
Lucero
P
P
Instituto de Investigaciones Biomédicas de CSIC, Madrid, Spain.
Herweijer
M
M
Lagunas
R
R
D000906:Antibodies / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D008320:Maltose / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters
D000906:Antibodies; D002352:Carrier Proteins; D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C067575:maltose transport system, S cerevisiae; D008320:Maltose