The mini-exon gene: a molecular marker for Endotrypanum schaudinni.
The mini-exon gene repeats from three different strains of Endotrypanum schaudinni have been amplified by the polymerase chain reaction (PCR). Sequence analysis of the cloned products shows the gene and intergenic region to be identical in two of the strains (LV86 and M6159); the intergenic region from the mini-exon gene of the third strain (LV59) is significantly different. The LV86 gene and an intergenic probe from the LV59 mini-exon gene do not cross-hybridize with the mini-exon gene from New World Leishmania species. These data provide the basis of a PCR assay to detect E. schaudinni and distinguish it from New World Leishmania species, and which should be applicable to epidemiological studies in insect vectors and mammalian reservoirs. The identification of two different mini-exon gene repeats in E. schaudinni isolates is indicative of further strain variation within this species.
Fernandes
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Department of Microbiology and Immunology, University of California, Los Angeles 90024.
Degrave
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Campbell
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D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005091:Exons / Q000235:genetics*; D017125:Genes, Protozoan; D005819:Genetic Markers; D007891:Leishmania / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014351:Trypanosomatina / Q000235:genetics*
D017931:DNA Primers; D016054:DNA, Protozoan; D005819:Genetic Markers