Autoradiographic analysis of (14C-methyl)-L-methionine uptake in transplanted rat brain tumors.
To elucidate metabolic pathways of methionine in brain tumors, we studied autoradiographic images of (14C-methyl)-L-methionine (14C-Met) in transplanted rat brain tumors. 14C-Met accumulated in the tumor almost twice as much as in the gray matter 90 min after injection. The acid-insoluble fraction, which was bound to macromolecules, comprised 84% the total count of the accumulated tracers in the tumor. Analysis of 14C-Met tracer accumulation is informative for understanding amino acid metabolism in brain tumors.
Sasajima
T
T
Neurosurgical Service, Akita University Hospital.
Mineura
K
K
Kuwahara
N
N
Kowada
M
M
Murakami
M
M
Uemura
K
K
D000818:Animals; D001345:Autoradiography; D001932:Brain Neoplasms / Q000378:metabolism*; D002250:Carbon Radioisotopes; D008297:Male; D008715:Methionine / Q000493:pharmacokinetics*; D009368:Neoplasm Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured / Q000637:transplantation
D002250:Carbon Radioisotopes; D008715:Methionine