title: A quantitative determination of singlet oxygen with horseradish peroxidase.
abstract: A singlet oxygen determination method based on the formation of dimethyl-(2,5)-2-methoxy-5-hydroperoxydihydrofuran (DMFO2) followed by acid hydrolysis with 0.1 N H2SO4 is described. Hydrogen peroxide is formed thereby as a product of hydrolysis which is then determined by the horseradish peroxidase-catalyzed formation of the homovanillic acid dimer. Since DMFO2 is a crystalline compound with sufficient stability it can be used as a primary standard for the determination. The peroxidase-catalyzed hydrogen peroxide determination allows detection of singlet oxygen concentrations as low as 10(-7) M.
authors.affiliation: Ludwig Boltzmann Institut für Klinische Onkologie und Photodynamik, Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Kreitner
authors.forename: G
authors.initials: G
authors.lastname: Alth
authors.forename: H
authors.initials: H
authors.lastname: Koren
authors.forename: S
authors.initials: S
authors.lastname: Loew
authors.forename: R
authors.initials: R
authors.lastname: Ebermann
mesh_terms: D005609:Free Radicals; D005663:Furans / Q000737:chemistry; D006415:Hematoporphyrins / Q000737:chemistry; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D000432:Methanol / Q000737:chemistry; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000737:chemistry; D012973:Sodium Hypochlorite / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
chemical_list: D005609:Free Radicals; D005663:Furans; D006415:Hematoporphyrins; D017382:Reactive Oxygen Species; D013481:Superoxides; C039316:potassium superoxide; D006861:Hydrogen Peroxide; C037555:2,5-dimethylfuran; D012973:Sodium Hypochlorite; D006735:Horseradish Peroxidase; D000432:Methanol