[The influence of DPDPE on intracellular free calcium concentration induced by Bt2 cAMP and phorbol ester].
The effect of DPDPE on intracellular free calcium concentration in neuroblastoma glioma hybrid cells (NG108-15) was studied with fura-2/AM fluorescence. The results were as follows: 1) The membrane permeable cAMP analogue Bt2cAMP, the adenylate cyclase activator forskolin and the protein kinase C activator TPA all induced an increase in intracellular free calcium concentration. 2) Verapamil suppressed the increase of [Ca2+]i induced by Bt2cAMP, forskolin and TPA. 3) DPDPE blocked the increase of [Ca2+]i induced by Bt2cAMP and forskolin, but not that induced by TPA. These results imply that DPDPE suppresses cyclic AMP-dependent protein kinase-induced but not protein kinase C-induced intracellular free calcium.
Sun
X
X
Institute of Basic Medical Sciences, Beijing.
D000252:Calcium-Transporting ATPases / Q000187:drug effects*; D005576:Colforsin / Q000037:antagonists & inhibitors; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000494:pharmacology*; D006801:Humans; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology
D004745:Enkephalins; D005576:Colforsin; D020881:Enkephalin, D-Penicillamine (2,5)-; D014700:Verapamil; D000242:Cyclic AMP; D000252:Calcium-Transporting ATPases; D013755:Tetradecanoylphorbol Acetate