title: Comparison of two clean-up methodologies for the gas chromatographic/mass spectrometric determination of low nanogram/gram levels of polynuclear aromatic hydrocarbons in seafood.
abstract: The March 1989 oil spill in Alaska prompted the Food and Drug Administration (FDA) to conduct a thorough investigation of clean-up methodologies aimed at determining low ng/g (ppb) levels of polynuclear aromatic hydrocarbons (PAHs) in seafood. The clean-ups from a modified FDA method and a National Marine Fisheries Service (NMFS) method were evaluated on the basis of the determination of 18 PAHs at levels ranging from 1 to 5 ppb by gas chromatography/mass spectrometry. In the modified FDA method, seafood extracts were purified by a liquid-liquid partition followed by a three-step elution through silica, alumina, and C18 solid-phase extraction cartridges. In the NMFS method, seafood extracts were purified by column chromatography through a deactivated silica gel/alumina column and a gel permeation high performance liquid chromatography column. Both methods quantitated 18 PAHs at levels ranging from 1 to 5 ppb. With the exception of naphthalene, average recoveries based on internal deuterated standards ranged from 73 to 144% for the modified FDA method and 63 to 106% for the NMFS method.
authors.affiliation: Food and Drug Administration, Division of Product Manufacture and Use, Washington, DC 20204.
authors.forename: P J
authors.initials: PJ
authors.lastname: Nyman
authors.forename: G A
authors.initials: GA
authors.lastname: Perfetti
authors.forename: F L
authors.initials: FL
authors.lastname: Joe
authors.forename: G W
authors.initials: GW
authors.lastname: Diachenko
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D011083:Polycyclic Compounds / Q000032:analysis*; D017747:Seafood / Q000032:analysis*
chemical_list: D011083:Polycyclic Compounds