Gelsolin-actin complex is target for ADP-ribosylation by Clostridium botulinum C2 toxin in intact human neutrophils.
The gelsolin-actin complex was ADP-ribosylated by Clostridium botulinum C2 toxin and Clostridium perfringens iota toxin in lysates of human platelets and human neutrophils. When [32P]orthophosphate-labelled human neutrophils were treated with C. botulinum C2 toxin, [32P]ADP-ribosylated gelsolin-actin was precipitated with anti-gelsolin antibody. Stimulation of neutrophils by formyl-methionine-leucine-phenylalanine decreased the interaction of gelsolin with ADP-ribosylated actin in toxin-treated cells. The data indicate that the gelsolin-actin complex is a pathophysiological substrate for actin-ADP-ribosylating toxins.
Just
I
I
Institut für Pharmakologie und Toxikologie, Universität des Saarlandes, Homburg-Saar, Germany.
Wille
M
M
Chaponnier
C
C
Aktories
K
K
D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D001905:Botulinum Toxins / Q000494:pharmacology*; D018260:Gelsolin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism
D000199:Actins; D018260:Gelsolin; D000246:Adenosine Diphosphate Ribose; D001905:Botulinum Toxins