Monitoring tobacco smoke-induced DNA damage by 32P-postlabelling.
Adduct levels in smokers are substantial, i.e. about 1 adduct in 10(7) nucleotides in lung DNA of lung cancer patients. Tobacco smoke-associated (TSA) DNA adducts, formed in a dose- and exposure time-dependent manner in smokers' lung, persist for considerable periods of time, consistent with epidemiological evidence showing quantitative associations of smoking with lung cancer risk and mortality. These results support a causal relationship between such adducts and tobacco smoking-induced cancer. TSA adducts were also present in various other target organs for tobacco-smoke carcinogenesis, including kidney, bladder, oesophagus and pancreas.
Randerath
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Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030.
Randerath
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D000818:Animals; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D008168:Lung / Q000737:chemistry; D051379:Mice; D010761:Phosphorus Radioisotopes; D051381:Rats; D012907:Smoking / Q000009:adverse effects* / Q000378:metabolism*
D010761:Phosphorus Radioisotopes; D004247:DNA