Protection from ultraviolet irradiation by melanin of mosquitocidal activity of Bacillus thuringiensis var. israelensis.
A process for production, isolation, and purification of melanin produced by the fermentation of Streptomyces lividans 66 harboring a recombinant plasmid pIJ702-bearing tyrosinase gene has been developed. The efficacy of melanin in the protection of mosquito larvacidal activity of Bacillus thuringiensis var. israelensis against uv light has been studied. Results obtained by the live cell counts and the bioassay of residual mosquitocidal activity of B. thuringiensis var. israelensis after exposure to uv radiation showed that melanin is an excellent photoprotective agent.
Liu
Y T
YT
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Republic of China.
Sui
M J
MJ
Ji
D D
DD
Wu
I H
IH
Chou
C C
CC
Chen
C C
CC
D000330:Aedes / Q000254:growth & development; D000818:Animals; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D001427:Bacterial Toxins; D001681:Biological Assay; D004731:Endotoxins / Q000096:biosynthesis / Q000633:toxicity; D005822:Genetic Vectors; D006460:Hemolysin Proteins; D007814:Larva; D008543:Melanins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology* / Q000528:radiation effects; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D009032:Mosquito Control / Q000379:methods*; D010572:Pest Control, Biological / Q000379:methods*; D010777:Photochemistry; D011836:Radiation Tolerance / Q000187:drug effects; D013171:Spores, Bacterial; D013302:Streptomyces / Q000235:genetics*; D014466:Ultraviolet Rays
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D008543:Melanins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D014442:Monophenol Monooxygenase