title: Time course changes in cytoskeletal structures of cultured endothelial cells exposed to shear stress.
abstract: Changes in patterns of microfilament distribution were analyzed using cultured porcine aortic endothelial cells under shear flow. Within the elongated endothelial cells after shear flow exposure (20 dyne/cm2, 0-24 h), F-actin filaments were mainly observed as stress fibers. After 24 h exposure, the fluorescence intensity of rhodamine-phalloidin-stained cells was enhanced, indicating the increase in F-actin content. It was suggested that not only F-actin distribution but also the content may be affected by shear stress at the early stage of exposure.
authors.affiliation: Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ookawa
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: N
authors.initials: N
authors.lastname: Ohshima
mesh_terms: D008841:Actin Cytoskeleton / Q000473:pathology; D000199:Actins / Q000032:analysis; D000818:Animals; D001012:Aorta, Abdominal / Q000737:chemistry / Q000473:pathology; D001013:Aorta, Thoracic / Q000737:chemistry / Q000473:pathology; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000032:analysis; D003599:Cytoskeleton / Q000473:pathology*; D004730:Endothelium, Vascular / Q000737:chemistry / Q000473:pathology*; D008856:Microscopy, Fluorescence; D010784:Photomicrography; D012212:Rheology; D013314:Stress, Mechanical; D013552:Swine; D013997:Time Factors
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins