title: The changes of c-fos mRNA expression in the protein kinase C activated-osteoblastic MC3T3-E1 cells cultured in a low-calcium environment.
abstract: The effect of phorbol 12-myristate 13-acetate (TPA) on the c-fos mRNA expression in the osteoblastic MC3T3-E1 cells cultured in a low-calcium environment was examined. The c-fos mRNA expression in MC3T3-E1 cells with the treatment of TPA was enhanced under the low-calcium environment. This fact suggests that TPA induced-protein kinase C enhancement produced the increase of c-fos mRNA expression, and that protein kinase C may be intimately involved in c-fos mRNA expression.
authors.affiliation: Department of Dental Pharmacology, School of Dentistry, Hokkaido University, Sapporo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Deyama
authors.forename: M
authors.initials: M
authors.lastname: Okitsu
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Hisada
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D004789:Enzyme Activation; D010006:Osteoblasts / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D003470:Culture Media; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium