title: Human complement factor B: functional properties of a recombinant zymogen of the alternative activation pathway convertase.
abstract: The human complement factor B is a centrally important component of the alternative pathway activation of the complement system. Here we report the isolation, characterization and eukaryotic expression of the first full length cDNA transcript for human factor B. In a factor B dependent haemolysis assay, the recombinant human factor B generated by transient COS cell transfection was shown to reconstitute haemolytic activity of factor B depleted human serum. To study the biological activities assigned to factor B, the availability of recombinant polypeptides representing definite portions of the human factor B molecule is desirable.
authors.affiliation: First Medical Clinic, University Hospital, Mainz, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Schwaeble
authors.forename: B
authors.initials: B
authors.lastname: Lüttig
authors.forename: T
authors.initials: T
authors.lastname: Sokolowski
authors.forename: C
authors.initials: C
authors.lastname: Estaller
authors.forename: E H
authors.initials: EH
authors.lastname: Weiss
authors.forename: K H
authors.initials: KH
authors.lastname: Meyer zum Büschenfelde
authors.forename: K
authors.initials: K
authors.lastname: Whaley
authors.forename: W
authors.initials: W
authors.lastname: Dippold
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D050577:Complement C3-C5 Convertases / Q000235:genetics* / Q000378:metabolism; D011415:Complement Factor B / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015941:Complement Hemolytic Activity Assay; D003170:Complement Pathway, Alternative; D004247:DNA / Q000302:isolation & purification; D004792:Enzyme Precursors / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D004792:Enzyme Precursors; D011994:Recombinant Proteins; D004247:DNA; D050577:Complement C3-C5 Convertases; D011415:Complement Factor B