title: Transcriptional activation of Mn-superoxide dismutase gene (sodA) of Escherichia coli by MnCl2.
abstract: Transcription of the manganese-superoxide dismutase gene (sodA) in Escherichia coli was shown to be activated by manganese. Addition of MnCl2 increased the expression of beta-galactosidase from a sodA::lacZ protein fusion and increased the concentration of mRNA transcribed from sodA+ and sodA::lacZ constructs. The stimulatory affect of manganese on the expression of sodA::lacZ was greatly reduced (i.e., > 90%) in a strain harboring a fur mutation. We also found that manganese was capable of altering DNA topology. These results show that Mn2+ causes activation of sodA transcription.
authors.affiliation: Department of Biochemistry, North Carolina State University, Raleigh 27695-7622.
authors.forename: L W
authors.initials: LW
authors.lastname: Schrum
authors.forename: H M
authors.initials: HM
authors.lastname: Hassan
mesh_terms: D002712:Chlorides / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D007501:Iron / Q000032:analysis; D008345:Manganese / Q000032:analysis; D017895:Manganese Compounds / Q000494:pharmacology*; D009154:Mutation; D013482:Superoxide Dismutase / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D002712:Chlorides; D017895:Manganese Compounds; D008345:Manganese; D007501:Iron; D013482:Superoxide Dismutase; D001616:beta-Galactosidase; C025340:manganese chloride