[Electrophoretic analysis of isoenzyme and protein patterns of Romanomermis yunanensis and R. culicivorax (Nematoda:Mermithidae)].
Isoenzyme electrophoretic patterns of lactate dehydrogenase (LDH), malate dehydrogenase (MDH), esterase (EST) and peroxidase (PO) of post-parasitic juveniles and adults of Romanomermis yunanensis and R. culicivorax were analysed by disc electrophoresis, respectively. The protein patterns of the two species of Romanomermis were compared by vertical slab SDS polyacrylamide gel electrophoresis. The results showed marked differences in isoenzyme patterns (number of bands and/or Rf) of LDH and MDH between the two species of Romanomermis, but the similar positions of isoenzyme bands denoted a certain genetic relationship between them. No band was found in the isoenzyme patterns of PO and EST. Distinct differences in protein pattern were observed between the two species of Romanomermis, but some common bands in pattern reflected the phylogenetic relationships of these species. The differences of isoenzyme and protein patterns observed in this study have provided the reason for identifying and differentiating the two species of Romanomermis at the molecular level.
Cai
Q
Q
Peng
Y
Y
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis; D015801:Helminth Proteins / Q000032:analysis / Q000145:classification; D007527:Isoenzymes / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis*; D008632:Mermithoidea / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D013045:Species Specificity
D015801:Helminth Proteins; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D004950:Esterases