[Effect of lipopolysaccharide on cytosolic free calcium concentration in myeloma cells].
The concentration of cytosolic free calcium was measured using the fluorescent Ca(2+)-indicator quin 2, and the effect of lipopolysaccharide (LPS) on cytosolic free calcium in myeloma cells was studied. Incubation of myeloma cells with LPS elicited a significant increase in cytosolic free calcium, and inhibited the phytohaemagglutinin (PHA)-induced elevation in cytosolic free calcium. Treatment of the cells with phorbol-12-myristate-13-acetate (PMA) failed to affect the concentration of cytosolic free calcium in resting cells, but inhibited the subsequent PHA-induced elevation in cytosolic free calcium. It is suggested that LPS causes alterations in intracellular calcium homeostasis and perturbations in the calcium-dependent signal transduction pathway.
Xiao
D
D
Institute of Basic Medical Sciences, Beijing.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D009101:Multiple Myeloma / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D008070:Lipopolysaccharides; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium