[Monoclonal Anti-Id as substitute for HBsAg to detect Anti-HBs].
According to Jerne's idiotype-anti-idiotype network theory, a new method to detect Anti-HBs was studied. This method utilized monoclonal Anti-Id as a substitute for HBsAg of the routine method adopting the enzyme-linked immunosorbent assay (ELISA) format. The serum specimen was incubated with solid-phase (polyvinyl chloride) that had been coated with monoclonal Anti-Id. If Anti-HBs was present in the serum, it would be combined with the Anti-Id. HRP-labeled goat Anti-human IgG was added, which reacted with the immobilized Anti-HBs. Under optimal conditions, 0.5 microgram/ml Anti-HBs could be detected. This method is safe, simple, and specific for Anti-HBs with no cross-reactivity with Anti-HBs, Anti-HBe and rheumatic factor positive serum, and the reagents can be obtained in unlimited amounts and are homogeneous. These features are particularly attractive when the antigen is difficult to obtain.
Li
H
H
Wang
D
D
Mu
J
J
D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens