Tissue distribution of cytochrome c oxidase isoforms in mammals. Characterization with monoclonal and polyclonal antibodies.
Monoclonal and polyclonal antibodies specific to the two isoforms of subunit VIa of bovine cytochrome c oxidase were generated and used to study the tissue distribution of this subunit pair in beef, human and rat. The so-called H-(heart) form was found exclusively in heart and skeletal muscle, whereas the so-called L-(liver) form was the only isoform present in brain, kidney, liver and smooth muscle. Little or no L-form was detected in skeletal muscle. In bovine heart no subunit VIa-L was detected, while in human heart the subunit VIa-H and VIa-L isoforms were present in roughly equal proportions. These results imply that, in humans, the deficiency of a subunit VIa isoform may have a different effect on the physiology of heart then on the physiology of skeletal muscle.
Taanman
J W
JW
Institute of Molecular Biology, University of Oregon, Eugene 97403.
Hall
R E
RE
Tang
C
C
Marusich
M F
MF
Kennaway
N G
NG
Capaldi
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001921:Brain / Q000201:enzymology; D002417:Cattle; D003576:Electron Transport Complex IV / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D007527:Isoenzymes / Q000032:analysis*; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000201:enzymology*; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D051381:Rats
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D003576:Electron Transport Complex IV