title: The gp120 glycoprotein of human immunodeficiency virus type 1 binds to sensory ganglion neurons.
abstract: Using immunofluorescence microscopy we found that gp120 binds to the surface of rat dorsal root ganglia neurons and human neuroblastoma cells but not to rat fibroblasts or glial cells. The binding of gp120 to neurons was eliminated by pretreatment with trypsin, which removes cell-surface proteins, but not with chloroform: methanol, which removes glycolipids. As control, neuronal staining by antisulfatide antibodies was eliminated by pretreatment with chloroform: methanol but not with trypsin. The gp120 binding to neurons was also inhibited by the mouse monoclonal antibody 01, which binds to galactocerebroside and cross-reactive glycoproteins. These studies suggest that the receptor for gp120 on the surface of the dorsal root ganglia neurons is a glycoprotein. This interaction may mediate the effects of human immunodeficiency virus type 1 in sensory neuropathy.
authors.affiliation: Department of Neurology, Columbia University-College of Physicians and Surgeons, New York, NY 10032.
authors.forename: S
authors.initials: S
authors.lastname: Apostolski
authors.forename: T
authors.initials: T
authors.lastname: McAlarney
authors.forename: A
authors.initials: A
authors.lastname: Quattrini
authors.forename: S W
authors.initials: SW
authors.lastname: Levison
authors.forename: G
authors.initials: G
authors.lastname: Rosoklija
authors.forename: A
authors.initials: A
authors.lastname: Lugaressi
authors.forename: M
authors.initials: M
authors.lastname: Corbo
authors.forename: S A
authors.initials: SA
authors.lastname: Sadiq
authors.forename: S
authors.initials: S
authors.lastname: Lederman
authors.forename: A P
authors.initials: AP
authors.lastname: Hays
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005727:Ganglia, Spinal / Q000378:metabolism*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D015497:HIV-1; D006801:Humans; D008856:Microscopy, Fluorescence; D009475:Neurons, Afferent / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
chemical_list: D015699:HIV Envelope Protein gp120