Stimulation of plasmin activity in cultured human fibroblast cells by Porphyromonas endodontalis.
1. Plasmin activity in the conditioned medium of Gin-1 cells, a human gingival fibroblast cell line, was stimulated by Porphyromonas endodontalis, a putative pathogen of oral submucous abscesses, in a time- and dose-dependent manner. 2. P. endodontalis stimulated the activity of plasminogen activator in both the conditioned medium and the cell lysate. The plasminogen activator in Gin-1 cells was approx. 50 kDa by zymography. 3. The conditioned medium of Gin-1 cells exposed to P. endodontalis stimulated the conversion of human serum prekallikrein to kallikrein. 4. These results suggested that P. endodontalis stimulates the plasminogen activator-plasmin system in Gin-1 cells, and that activated plasmin plays a role in the progress of periodontal tissue inflammation.
Oikawa
T
T
Department of Oral Surgery, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Ogura
N
N
Akiba
M
M
Abiko
Y
Y
Takiguchi
H
H
Izumi
H
H
D000595:Amino Acid Sequence; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005341:Fibrinolysin / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010960:Plasminogen Activators / Q000378:metabolism*; D017861:Porphyromonas / Q000502:physiology*; D011288:Prekallikrein / Q000187:drug effects
D011288:Prekallikrein; D010960:Plasminogen Activators; D005341:Fibrinolysin