title: Yield of strand breaks as a function of scavenger concentration and LET for SV40 irradiated with 4He ions.
abstract: We have measured by gel electrophoresis the yields of single- and double-strand breaks (SSBs and DSBs) induced in aqueous solutions of SV40 DNA and the SV40 minichromosome by 137Cs gamma rays (mean LET 0.3 keV micron-1) and 4He ions (mean LETs 85, 102, and 152 keV microns-1). DNA SSBs are caused mainly by the hydroxyl radicals under these conditions and are reduced in yield as either the hydroxyl radical scavenger concentration or the LET is increased (over the range studied). The G(SSB) for 4He ion irradiation is less by a factor of up to 10 than the G(SSB) for gamma irradiation, depending upon the scavenger concentration. The difference in the yields of SSBs agrees well with the difference in the yields of hydroxyl radicals for the radiations in question. In contrast, the yields of DSBs are similar for gamma and 4He ion irradiation over much of the range of scavenging capacity studied. However, at the highest scavenger concentrations the yields of DSBs are greater for 4He ion irradiation. In addition, the yields of DSBs remain almost constant with increasing LET (over the range studied). Therefore the relative yield of DSBs per SSB increases with increasing LET, supporting the hypothesis that increasing LET leads to an increased clustering of damage in DNA.
authors.affiliation: Department of Radiology-0610, University of California at San Diego, La Jolla 92093-0610.
authors.forename: G D
authors.initials: GD
authors.lastname: Jones
authors.forename: J R
authors.initials: JR
authors.lastname: Milligan
authors.forename: J F
authors.initials: JF
authors.lastname: Ward
authors.forename: P M
authors.initials: PM
authors.lastname: Calabro-Jones
authors.forename: J A
authors.initials: JA
authors.lastname: Aguilera
mesh_terms: D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004735:Energy Transfer; D016166:Free Radical Scavengers; D005720:Gamma Rays; D006371:Helium / Q000494:pharmacology*; D006657:Histones / Q000528:radiation effects; D013539:Simian virus 40 / Q000235:genetics / Q000528:radiation effects
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D016166:Free Radical Scavengers; D006657:Histones; D006371:Helium; D004247:DNA; D004121:Dimethyl Sulfoxide