N sequences, P nucleotides and short sequence homologies at junctional sites in VH to VHDJH and VHDJH to JH joining.
Junctional sequences in VH to VHDJH and VHDJH to JH joining occurring in Abelson virus-transformed immature B cell lines were PCR-amplified and sequenced. In VH to VHDJH joining, 24 (23%) out of 105 junctions examined here had P nucleotides and/or N sequences, and out of the remaining 81 junctions without P nucleotides and N sequences, 57 (70%) had short sequence homologies of one or two bases (A, C, G, CA or AG) and three had long sequence homologies at the junctional sites. In VHDJH to JH joining, 38 (43%) out of 89 junctions examined here had P nucleotides and/or N sequences, and out of the remaining 51 junctions without P nucleotides and N sequences, 47 (92%) had short sequence homologies of one or two bases (C, T, G, TG or GG) at the junctional sites. These results indicate that short sequence homologies play an important role for end joining in VH to VHDJH and VHDJH to JH joining.
Komori
T
T
Osaka University Medical School, Department of Medicine III, Suita City, Japan.
Sugiyama
H
H
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D001483:Base Sequence; D002461:Cell Line, Transformed; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D007143:Immunoglobulin Heavy Chains; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region