title: Porphyromonas endodontalis: prevalence and distribution of restriction enzyme patterns in families.
abstract: In this study we determined the prevalence and distribution of Porphyromonas endodontalis in 26 families consisting of 107 subjects. P. endodontalis was present in 24% of the investigated subjects and was recovered most often from the dorsum of the tongue (50%). Isolation was also possible from the tonsils, the buccal mucosa, the saliva and the periodontal pocket. The usefulness of restriction endonuclease analysis as a typing method for this particular species was investigated by typing 19 isolates from unrelated individuals. All these isolates had unique restriction endonuclease patterns. The observed heterogeneity indicates that restriction endonuclease analysis is a sensitive measure of genetic dissimilarity between P. endodontalis isolates and is able to characterize individual isolates. Application of restriction endonuclease analysis to the obtained clinical isolates in this study shows the possibility of the presence of multiple clonal types within one subject. The DNA patterns of all P. endodontalis isolates from unrelated individuals were found to be distinct. In 3 families the DNA patterns of isolates from the mother and her child were indistinguishable. These data indicate the possibility of intrafamilial transmission of P. endodontalis.
authors.affiliation: Department of Periodontology, Academic Centre for Dentistry, Amsterdam, Netherlands.
authors.forename: M D
authors.initials: MD
authors.lastname: Petit
authors.forename: A J
authors.initials: AJ
authors.lastname: van Winkelhoff
authors.forename: T J
authors.initials: TJ
authors.lastname: van Steenbergen
authors.forename: J
authors.initials: J
authors.lastname: de Graaff
mesh_terms: D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes; D005190:Family; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D017861:Porphyromonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015995:Prevalence
chemical_list: D004262:DNA Restriction Enzymes