Cell lineage involvement in four patients with myelodysplastic syndrome and t(1;7) or trisomy 8 studied by simultaneous immunophenotyping and fluorescence in situ hybridization.
Four patients with myelodysplastic syndrome (MDS), one with t(1;7) and three with trisomy 8, were studied by immunophenotyping and fluorescence in situ hybridization (FISH) to assess cell lineage involvement. The t(1;7) was detected using a biotin-labeled chromosome 1 centromere-specific DNA probe. This aberration was present in CD34-positive stem cells, the erythroid cell lineage (GPA+), and the granulocytic/monocytic (CD13+ and CD64+) cell lineages. We were not able to demonstrate the abnormality in the lymphoid cell lineages. In the patients with trisomy 8, the aberration was detected with chromosome 8 centromere-specific DNA probe or by chromosome in situ suppression hybridization (CISS) with a chromosome 8-specific library probe. The trisomy was detected in stem cells, erythroid precursor cells, megakaryocytes, and granulocytes/monocytes. In these MDS patients, the chromosome aberrations appear to occur only in cells of myeloid lineage.
Nylund
S J
SJ
Department of Medical Genetics, University of Helsinki, Finland.
Verbeek
W
W
Larramendy
M L
ML
Ruutu
T
T
Heinonen
K
K
Hallman
H
H
Knuutila
S
S
D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis; D002878:Chromosomes, Human, Pair 1; D002897:Chromosomes, Human, Pair 7; D002898:Chromosomes, Human, Pair 8; D015342:DNA Probes; D015672:Erythroid Precursor Cells / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D015723:Gene Library; D006021:Glycophorins / Q000032:analysis; D006098:Granulocytes / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008533:Megakaryocytes / Q000648:ultrastructure; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000648:ultrastructure; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000276:immunology; D013234:Stem Cells / Q000276:immunology / Q000648:ultrastructure; D014178:Translocation, Genetic; D014314:Trisomy
D015703:Antigens, CD; D015342:DNA Probes; D006021:Glycophorins