Aspects of schizandrin metabolism in vitro and in vivo.
The metabolic transformation of schizandrin, isolated from the kernel of Schizandra chinensis Bill, was studied in vitro with a phenobarbital induced rat liver microsomal fraction containing the NADPH generating system. The major metabolites were isolated by preparative HPLC and identified as 7,8-dihydroxy-schizandrin, 7,8-dihydroxy-2-demethyl-schizandrin, and 7,8-dihydroxy-3-demethyl-schizandrin by UV, NMR and MS spectral analysis. The 7,8-dihydroxy-schizandrin was confirmed further by comparison with spectral and chromatographic behavior of the authentic compound. The metabolic biotransformation of schizandrin in vivo was also determined.
Cui
Y Y
YY
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Wang
M Z
MZ
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D034242:Cyclooctanes; D066298:In Vitro Techniques; D017705:Lignans / Q000378:metabolism* / Q000493:pharmacokinetics*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009249:NADP / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D010634:Phenobarbital / Q000494:pharmacology; D010946:Plants, Medicinal; D011083:Polycyclic Compounds / Q000378:metabolism* / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet
D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; D009249:NADP; C011105:schizandrin; D010100:Oxygen; D010634:Phenobarbital