title: Analysis of accessory signaling in human T-cell clones.
abstract: Human T cells, when activated, express small but detectable levels of MHC class II on their surface and as a result have the potential to present antigens in the context of MHC class II molecules. There are reports demonstrating MHC class II restricted antigen presentation by human T-cell clones. In this report, we show evidence for one such clone that can present peptide antigen to itself. This clone, HA1.7 ml, is clearly different in its accessory signaling requirements from the parental clone HA1.7, and shows a decrease in the surface level of CD54. We have analyzed the accessory signaling abilities of T cells by using HA1.7 ml as a responder population, and demonstrate that the accessory signaling potential of T-cell clones is sufficient to bring about activation of HA1.7 ml but not of HA1.7. This accessory signaling ability is, however, different from that of B cells in providing bystander accessory signals. T-cell APCs cannot provide bystander accessory signals for the proliferative response of HA1.7 ml, nor can they block the induction of tolerance in this subline, whereas bystander B cells can mediate both events to a limited extent. Thus, there are significant differences in the accessory signaling abilities of T cells as APCs as compared with classic APCs such as B cells, independent of their ability to generate peptide-MHC complexes. It also appears that although HA1.7 ml can be activated by self-presentation of antigen, it is still susceptible to the induction of tolerance.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Immunology, Royal Postgraduate Medical School, London, England.
authors.forename: V
authors.initials: V
authors.lastname: Bal
authors.forename: J
authors.initials: J
authors.lastname: Lamb
authors.forename: R
authors.initials: R
authors.lastname: Lechler
mesh_terms: D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001324:Autoantigens