title: Phenotypic alteration of glioma cells during culture.
abstract: Tissue specimens and culture cells from three human gliomas (two astrocytomas and one glioblastoma) were immunohistochemically investigated, using GFAP, S-100P, vimentin, FN and TNF antibodies. Primary culture consisted of two cell types, flat cells and fibrous cells. Phenotypic alternation was observed during successive subculture. Differences between fibrous cells in astrocytoma and those in glioblastoma were remarkable, while flat cells in astrocytoma and glioblastoma examined in this study, were similar.
authors.affiliation: Department of Neurosurgery, School of Medicine, Fujita Health University.
authors.forename: F
authors.initials: F
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Marunouchi
authors.forename: Y
authors.initials: Y
authors.lastname: Mizoguchi
authors.forename: M
authors.initials: M
authors.lastname: Ogasawara
authors.forename: T
authors.initials: T
authors.lastname: Kanno
mesh_terms: D000328:Adult; D001254:Astrocytoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002648:Child; D005353:Fibronectins / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010641:Phenotype; D009418:S100 Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D005353:Fibronectins; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; D014409:Tumor Necrosis Factor-alpha; D014746:Vimentin