title: Absorption, tissue distribution and excretion of radiolabelled compounds in rats after administration of [14C]-L-alpha-glycerylphosphorylcholine.
abstract: The kinetics and metabolism of L-alpha-glycerylphosphoryl-choline (alpha-GPC) were investigated in male and female rats after i.v. (10 mg/kg) and oral doses (100-300 mg/kg). alpha-GPC was labelled with [14C]-glycerol ([14G]-GPC) or [14C]-choline ([14C]-GPC). Different kinetic and metabolic profiles were observed after i.v. and oral administration. It is assumed that alpha-GPC is hydrolyzed by phosphodiesterases in the gut mucosa. The different labelled metabolites have different kinetic properties of absorption, distribution and clearance, leading to different blood concentration-time curves of total radioactivity. Both labelled compounds gave a wide distribution of radioactivity, particularly concentrated in the liver, kidney, lung and spleen compared to blood. Brain concentrations of [14C]-GPC were comparable to ([14G]-GPC) or lower than ([14C]-GPC) total blood radioactivity. The metabolite profile in the perfused brain showed a small amount of choline and two unknown metabolites, probably the same as in blood. In addition, choline was incorporated into brain phospholipids in increasing amounts within 24 h of dosing. In all cases renal and fecal excretion of radioactivity was low and comparable for [14G]-GPC and [14C]-GPC. Mostly the administered radioactivity was exhaled as 14CO2, this degradation being faster and more pronounced for the glycerol-labelled metabolites than for the choline-labelled metabolites for both routes of administration. In all cases the results were the same for male and female rats.
authors.affiliation: LPB Research Institute, Cinisello B., Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Abbiati
authors.forename: T
authors.initials: T
authors.lastname: Fossati
authors.forename: G
authors.initials: G
authors.lastname: Lachmann
authors.forename: M
authors.initials: M
authors.lastname: Bergamaschi
authors.forename: C
authors.initials: C
authors.lastname: Castiglioni
mesh_terms: D000042:Absorption; D000284:Administration, Oral; D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D002794:Choline / Q000493:pharmacokinetics; D005260:Female; D005990:Glycerol / Q000493:pharmacokinetics; D005997:Glycerylphosphorylcholine / Q000097:blood / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D002250:Carbon Radioisotopes; D005997:Glycerylphosphorylcholine; D002794:Choline; D005990:Glycerol