title: Effect of duodenal juice on bombesin-stimulated cholecystokinin release during loxiglumide administration in man.
abstract: Stimulation of cholecystokinin release by bombesin in augmented by cholecystokinin receptor blockade with loxiglumide. We hypothesize that this augmented cholecystokinin release results from inhibition of the pancreatico-biliary response to bombesin during cholecystokinin receptor blockade. To test this hypothesis, we infused bombesin for 180 min in six healthy subjects Three bombesin-infusion experiments were performed in each subject in random order on different days. In two of these experiments loxiglumide was co-infused with bombesin, while in the third experiment saline was co-infused with bombesin. In one of the loxiglumide experiments, duodenal juice, collected on the previous day during infusion of cholecystokinin-GIH, was reperfused intraduodenally during the second hour of bombesin infusion. In the saline experiment, the integrated cholecystokinin response during the first hour of bombesin-infusion (262 +/- 63 pmol 60 min-1) was significantly (P < 0.01) higher than during the second (88 +/- 26 pmol 60 min-1) and third (87 +/- 31 pmol 60 min-1) hour of bombesin-infusion. Loxiglumide augmented bombesin-stimulated cholecystokinin secretion from 262 +/- 63 pmol 60 min-1 to 453 +/- 63 pmol 60 min-1 in the first hour of bombesin infusion (P < 0.01). Integrated cholecystokinin values in the second (489 +/- 90 pmol 60 min-1) and third (450 +/- 74 pmol 60 min-1) hour of the loxiglumide experiment, were significantly (P < 0.01) higher than in the saline experiment.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Gastroenterology, University Hospital of Leiden, The Netherlands.
authors.forename: M C
authors.initials: MC
authors.lastname: Jebbink
authors.forename: J B
authors.initials: JB
authors.lastname: Jansen
authors.forename: F G
authors.initials: FG
authors.lastname: van der Kleij
authors.forename: D M
authors.initials: DM
authors.lastname: Mooy
authors.forename: L C
authors.initials: LC
authors.lastname: Rovati
authors.forename: C B
authors.initials: CB
authors.lastname: Lamers
mesh_terms: D000328:Adult; D001839:Bombesin / Q000008:administration & dosage*; D002766:Cholecystokinin / Q000097:blood* / Q000187:drug effects; D005260:Female; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D010189:Pancreatic Juice / Q000502:physiology*; D011377:Proglumide / Q000008:administration & dosage / Q000031:analogs & derivatives*
chemical_list: C053737:loxiglumide; D002766:Cholecystokinin; D011377:Proglumide; D001839:Bombesin