Sites of photodamage by the iminium salt of a copper octaethylbenzochlorin.
Because the benzochlorin derivative copper (II) alpha-meso-N,N'-dimethyloctaethylbenzochlorin iminium chloride (CDS1) is not fluorescent, sites of drug localization in L1210 cells were detected by indirect methods involving using a series of fluorescent probes. The CDS1-mediated cytotoxicity was associated with mitochondrial damage, a decreased membrane potential and an increase in the heterogeneity of membrane sites of binding of a polar analog of diphenylhexatriene. Although CDS1 is a cationic compound, its accumulation was not impaired in a cell line exhibiting the multidrug resistance phenotype.
Kessel
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Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003905:Deuteroporphyrins / Q000378:metabolism / Q000633:toxicity*; D004317:Doxorubicin / Q000633:toxicity; D004351:Drug Resistance; D007097:Imines / Q000378:metabolism / Q000633:toxicity*; D007939:Leukemia L1210; D007941:Leukemia P388; D008077:Lipoproteins, LDL / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000633:toxicity*; D011485:Protein Binding; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003905:Deuteroporphyrins; D007097:Imines; D008077:Lipoproteins, LDL; D017319:Photosensitizing Agents; C081504:copper benzochlorin; D004317:Doxorubicin