Both mannose and beta-glucan receptors are involved in phagocytosis of unopsonized, heat-killed Saccharomyces cerevisiae by murine macrophages.
We studied the involvement of lectin-like receptors in phagocytosis of unopsonized heat-killed yeast (Saccharomyces cerevisiae) by murine macrophage-like cell lines and murine peritoneal resident macrophages. For this purpose we used a technique that allowed us to discriminate ingested and adsorbed heat-killed yeast. The internalization can be partly inhibited by soluble polyosides such as laminarin (beta-glucan) or alpha-mannan. However, when they were used together (0.4 mg/ml alpha-mannan and 0.4 mg/ml laminarin), almost complete inhibition of phagocytosis was obtained. These observations suggest that phagocytosis of unopsonized heat-killed yeast by murine macrophage-like cell lines as well as murine peritoneal resident macrophages is mediated by both mannose and beta-glucan receptors. The respective activity of these two types of receptors is a function of in vitro cell differentiation. To achieve maximal phagocytosis of unopsonized heat-killed yeast, coexpression of both mannose and beta-glucan receptors is required.
Giaimis
J
J
Université Louis Pasteur, Département d'Immunologie, d'Immunopharmacologie et de Pathologie, Illkirch, France.
Lombard
Y
Y
Fonteneau
P
P
Muller
C D
CD
Levy
R
R
Makaya-Kumba
M
M
Lazdins
J
J
Poindron
P
P
D000818:Animals; D016923:Cell Death; D002460:Cell Line; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000494:pharmacology; D005936:Glucans / Q000378:metabolism; D006358:Hot Temperature; D037181:Lectins, C-Type; D008264:Macrophages / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology; D008351:Mannans / Q000494:pharmacology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000187:drug effects*; D011134:Polysaccharides / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000502:physiology*; D011971:Receptors, Immunologic / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000276:immunology*
D005936:Glucans; D037181:Lectins, C-Type; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C045268:beta-glucan receptor; C008247:laminaran; D004693:Glucan Endo-1,3-beta-D-Glucosidase