Expression of receptors for advanced glycosylation end products on renal cell carcinoma cells in vitro.
Proteins that have been modified by long-term expose to glucose accumulate advanced glycosylation end products (AGEs) as a function of protein age. In these studies, we have examined the interaction of AGE-protein with renal cell carcinoma cells (RCC) in vitro, using AGE-modified bovine serum albumin (AGE-BSA) as a probe. AGE-BSA showed tendency to induce in vitro cell growth of RCC cells and promoted the production of interleukin-6 (IL-6), an in vitro autocrine growth factor. Reverse transcriptase-polymerase chain reaction analysis revealed that RCC cells used here express mRNA for a receptor for AGEs (RAGE). These results suggested that AGEs taken up through RAGE on RCC cells might play a role in promoting the growth of RCC cells.
Miki
S
S
Nissei Hospital, Osaka, Japan.
Kasayama
S
S
Miki
Y
Y
Nakamura
Y
Y
Yamamoto
M
M
Sato
B
B
Kishimoto
T
T
D018262:Adenocarcinoma, Clear Cell / Q000378:metabolism; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression; D017127:Glycation End Products, Advanced / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*
D017931:DNA Primers; D017127:Glycation End Products, Advanced; D015850:Interleukin-6; D012333:RNA, Messenger; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic