title: N-glycosylation of forms of angiotensin converting enzyme from four mammalian species.
abstract: To help clarify bases for the molecular weight and surface charge heterogeneities of forms of somatic angiotensin converting enzyme (ACE), we examined for differences in N-glycosylation. ACE preparations purified from human, guinea pig, rat and rabbit tissues were found to be heterogeneous in terms of numbers of N-glycosylated sites (7-8 sites per molecule of ACE) and in types of structures of oligosaccharides used for glycosylation (complex versus high mannose oligosaccharide contents). Our findings, taken with reports of potential N-glycosylation sites and amino acid sequencing data, indicate that ACE forms can differ in terms of degrees of glycosylation, sites of glycosylation and structures of attached oligosaccharide units.
authors.affiliation: Department of Medicine, University of Miami School of Medicine, FL 33101.
authors.forename: J E
authors.initials: JE
authors.lastname: Ripka
authors.forename: J W
authors.initials: JW
authors.lastname: Ryan
authors.forename: F A
authors.initials: FA
authors.lastname: Valido
authors.forename: A Y
authors.initials: AY
authors.lastname: Chung
authors.forename: C M
authors.initials: CM
authors.lastname: Peterson
authors.forename: R L
authors.initials: RL
authors.lastname: Urry
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006168:Guinea Pigs; D006801:Humans; D008168:Lung / Q000201:enzymology; D008297:Male; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D012661:Semen / Q000201:enzymology
chemical_list: D009844:Oligosaccharides; D003208:Concanavalin A; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D007703:Peptidyl-Dipeptidase A