[Reconstruction of a sequenced sequence using results of stacked hybridization with an oligonucleotide matrix].
The efficiency of additional rounds of continuous stacking hybridization in DNA sequence reconstruction by hybridization with oligonucleotide matrix (SHOM) is considered. At first, DNA is hybridized with a matrix of oligonucleotides of the length L. The overlapping of the tuples which have formed perfect duplexes with the DNA, on the one hand, has enabled us to reconstruct unambiguously a part of the sequence, and on the other hand, suggested a modified scheme for reconstructing the remaining part. Then the additional hybridizations should be carried out in the presence of shorter oligonucleotides of the length l, that are able to form perfect duplexes of the length L + l in continuous hybridization closely to matrix tuples. In this case the stability of a duplex consisting of l-tuples and long DNA is enhanced due to stacking interaction. The information obtained about the succession of L + l sites considerably increases the efficiency of reconstruction, which can eventually reach the efficiency of a matrix consisting of (L + l)-tuples. We propose here an algorithm for compiling such a set of l-tuples to be added that the number of additional hybridizations can be appreciably diminished. For an octanucleotide matrix and different sets of pentanucleotides to be used for continuous stacking hybridization, the length of unambiguously sequenced DNA undergoes a rise from 200 to some thousands bp.
Lysov
Iu P
IuP
Houston Advanced Research Center.
Chernyĭ
A A
AA
Balaev
A A
AA
Beattie
K L
KL
Florent'ev
V L
VL
Mirzabekov
A D
AD
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D013698:Templates, Genetic
D009838:Oligodeoxyribonucleotides; D004247:DNA
Rekonstruktsiia sekvenirumoĭ posledovatel'nosti po rezultatam gibridizatsii vstyk s oligonukleotidnoĭ matritseĭ.