The enhancer of position-effect variegation of Drosophila, E(var)3-93D, codes for a chromatin protein containing a conserved domain common to several transcriptional regulators.
In Drosophila modifying mutations of position-effect variegation have been successfully used to genetically dissect chromatin components. The enhancer of position-effect variegation E(var)3-93D [formerly E-var(3)3] encodes proteins containing a domain common to the transcriptional regulators tramtrack and the products of the Broad complex. It interacts with a number of chromatin genes that suppress position-effect variegation. Mutations in E(var)3-93D exhibit an imprinting-like effect on the Y chromosome. This effect is transmitted paternally over several generations. Homeotic transformations in E(var)3-93D mutants indicate an involvement of the gene products in regulation of homeotic gene complexes. An antiserum raised against E(var)3-93D protein detects this chromosomal protein in a large subset of sites in polytene chromosomes. Our genetic and molecular data suggest that the proteins of E(var)3-93D are generally involved in establishing and/or maintaining an open chromatin conformation.
Dorn
R
R
Institut für Genetik, Martin-Luther-Universität, Halle, Germany.
Krauss
V
V
Reuter
G
G
Saumweber
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000737:chemistry* / Q000648:ultrastructure; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D017344:Genes, Insect; D007173:Imprinting, Psychological; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
D002843:Chromatin; D004251:DNA Transposable Elements; D012333:RNA, Messenger; D014157:Transcription Factors