title: Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin.
abstract: The mechanism by which iron-storage proteins take up and oxidise iron(II) is not understood. We show by rapid-kinetic and EPR measurements that iron uptake, in vitro, by a bacterial iron-storage protein, bacterioferritin, involves at least three kinetically distinguishable phases: phase 1, the binding of Fe(II) ions, probably at a dimeric iron ferroxidase centre; phase 2, oxidation of the Fe(II) dimer and production of mononuclear Fe(III); and phase 3, iron core formation.
authors.affiliation: Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, UK.
authors.forename: N E
authors.initials: NE
authors.lastname: Le Brun
authors.forename: M T
authors.initials: MT
authors.lastname: Wilson
authors.forename: S C
authors.initials: SC
authors.lastname: Andrews
authors.forename: J R
authors.initials: JR
authors.lastname: Guest
authors.forename: P M
authors.initials: PM
authors.lastname: Harrison
authors.forename: A J
authors.initials: AJ
authors.lastname: Thomson
authors.forename: G R
authors.initials: GR
authors.lastname: Moore
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D003573:Cytochrome b Group / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli; D005293:Ferritins / Q000737:chemistry / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D007700:Kinetics
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D005293:Ferritins; C018032:bacterioferritin; D007501:Iron