Molecular cloning and chromosomal assignment of the gene for human Zn-alpha 2-glycoprotein.
Genomic clones containing the human Zn-alpha 2-glycoprotein gene were isolated. Two of them were for the functional gene overlapped, and the other four were for two different pseudogenes (1 and 2) retaining exon-intron organization. The complete DNA sequence of the functional gene (9.3 kb) and its 5'- and 3'-flanking regions (5.3 and 0.1 kb, respectively) was determined. The gene is composed of four exons; the first exon is for the 5'-untranslated region, the signal sequence, and the first six amino acids; the second, for domain A; the third, for domain B; and the fourth, for domain C and the 3'-untranslated region. The 5'-flanking region contains a TATA box, a CAT box, an octamer sequence, and three possible Spl-binding sites. Ten and three copies of Alu repetitive DNA were identified within the gene and the 5'-flanking region, respectively, and they occupy 30% of the gene. The nucleotide sequences around the exons of pseudogene 1 were also determined; they had high homology (90-91%) with the corresponding region of the functional gene. Southern blot analysis suggested that there are only three genes, including nonfunctional ones, for Zn-alpha 2-glycoprotein in humans. The gene (ZA2G) was mapped to human chromosome band 7q22.1 by fluorescence in situ hybridization.
Ueyama
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H
Department of Medical Biochemistry, Shiga University of Medical Science, Otsu, Japan.
Deng
H X
HX
Ohkubo
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I
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D005796:Genes; D015698:Genomic Library; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D011544:Pseudogenes; D029589:Seminal Plasma Proteins; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D000090203:Zn-Alpha-2-Glycoprotein
D006023:Glycoproteins; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein