title: Predominant expression of the beta subunit of prolyl 4-hydroxylase (disulfide isomerase) in human extravillous trophoblasts.
abstract: Prolyl 4-hydroxylase is a heterodimeric enzyme that is crucial in the biosynthesis of collagen. The beta subunit of this enzyme is a multifunctional protein which is also known as protein-disulfide isomerase. Immunofluorescence and monoclonal antibody (Mab) 5B5 were used to localize the beta subunit in human extraembryonic tissues. The strongest sites of 5B5 reactivity were extravillous cytotrophoblasts in the basal plate, uteroplacental arteries and amniochorion, syncytiotrophoblast displayed variable weaker reactivity. Only a small fraction of placental 5B5 antigen was detected as a component of prolyl-4-hydroxylase by affinity chromatography on immobilized polyproline. The results indicate a difference in the expression of an endoplasmic reticulum marker between villous and extravillous trophoblast. The predominance of 5B5 antigen in extravillous trophoblast could be associated with an increased ability to synthesize collagen or other enzymatic reactions associated with prolyl 4-hydroxylase beta subunit.
authors.affiliation: Center for Reproduction and Transplantation Immunology, Methodist Hospital of Indiana, Indianapolis 46202.
authors.forename: O A
authors.initials: OA
authors.lastname: Vanderpuye
authors.forename: C A
authors.initials: CA
authors.lastname: Labarrere
authors.forename: J A
authors.initials: JA
authors.lastname: McIntyre
mesh_terms: D000918:Antibody Specificity; D002824:Chorionic Villi / Q000201:enzymology*; D006801:Humans; D007535:Isomerases / Q000032:analysis* / Q000737:chemistry; D010920:Placenta / Q000166:cytology / Q000201:enzymology*; D019704:Protein Disulfide-Isomerases; D014327:Trophoblasts / Q000201:enzymology*
chemical_list: D007535:Isomerases; D019704:Protein Disulfide-Isomerases