Evidence for preferential repair of 3-carbethoxypsoralen plus UVA induced DNA lesions in the active MAT alpha locus in Saccharomyces cerevisiae using the UvrABC assay.
The occurrence of preferential repair in Saccharomyces cerevisiae of the active MAT alpha locus compared with the inactive HML alpha locus was confirmed after 254 nm UV irradiation. Experiments carried out using the UvrABC excinuclease assay with the monofunctional furocoumarin 3-carbethoxypsoralen (3-CPs) plus UVA radiation which induce mainly monoadducts in DNA demonstrated preferential repair of the active MAT alpha locus compared with the inactive HML alpha locus in a SIR+ strain. However, as after 254 nm UV irradiation, no difference in the rate of removal of 3-CPs plus UVA induced lesions was observed between the two loci in the sir-3 mutant in which both loci are active. Thus, it appears that 3-CPs plus UVA induced monoadducts as well as pyrimidine dimers are subject to preferential repair.
Méniel
V
V
Institut Curie, Section de Biologie, URA 1292 CNRS, Paris.
Brouwer
J
J
Averbeck
D
D
D001345:Autoradiography; D004260:DNA Repair / Q000235:genetics*; D004271:DNA, Fungal / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004706:Endodeoxyribonucleases; D029968:Escherichia coli Proteins; D011564:Furocoumarins / Q000378:metabolism / Q000633:toxicity*; D005800:Genes, Fungal / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D011740:Pyrimidine Dimers; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014158:Transcription, Genetic; D014466:Ultraviolet Rays / Q000009:adverse effects
D004271:DNA, Fungal; D029968:Escherichia coli Proteins; D011564:Furocoumarins; D011740:Pyrimidine Dimers; C017104:3-carbethoxypsoralen; D004706:Endodeoxyribonucleases; C036385:endodeoxyribonuclease uvrABC