Increased FISH efficiency using APC probes generated by direct incorporation of labeled nucleotides by PCR.
Probes of various sizes from the adenomatous polyposis coli gene (APC) were directly biotinylated by polymerase chain reaction (PCR) from genomic DNA. PCR labeling gave high efficiency in detection of fluorescence in situ hybridization (FISH) signals. Probes as small as 250 base pairs could be visualized through a fluorescence microscope without any image processing.
Richard
F
F
CNRS URA 620, Institut Curie, Section de Biologie, Paris, France.
Vogt
N
N
Muleris
M
M
Malfoy
B
B
Dutrillaux
B
B
D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D001483:Base Sequence; D001710:Biotin; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D004076:Digoxigenin; D005091:Exons; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015342:DNA Probes; D001710:Biotin; D004076:Digoxigenin