Cloning of VH and VL genes for antibodies specific for pyrimidine photo dimers.
We have prepared two types of cDNAs encoding mouse immunoglobulin heavy (VH) and light (VL) chain variable (V) domains against UV-induced DNA damage by the polymerase chain reaction (PCR). The amplified cDNA was cloned for sequencing and expression, and the nucleotide sequences of the V domains were determined. The deduced amino acid sequences of the complementarity-determining regions (CDRs) indicated that CDR3 of the VH domain and CDR2 of the VL domain might be concerned with the recognition of photolesions.
Morioka
H
H
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Miura
H
H
Kamiya
H
H
Matsunaga
T
T
Nikaido
O
O
Ohtsuka
E
E
D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011740:Pyrimidine Dimers / Q000276:immunology*; D014466:Ultraviolet Rays
D000906:Antibodies; D018076:DNA, Complementary; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D011740:Pyrimidine Dimers