title: Analysis of milk-based infant formula. Phase IV. Iodide, linoleic acid, and vitamins D and K: U.S. Food and Drug Administration-Infant Formula Council: collaborative study.
abstract: In 1982, the U.S. Food and Drug Administration, the Infant Formula Council and its member companies, contract laboratories, and other government laboratories began a study of analytical methods for the nutrients listed in the Infant Formula Act of 1980. Phases I, II, III, and V have been completed. The present report provides data on Phase IV, in which 13 laboratories collaboratively studied an ion-selective electrode method for analyzing iodide, a gas chromatographic method for linoleic acid, and 2 liquid chromatographic (LC) methods each for vitamins D and K. Data were insufficient to evaluate one each of the LC methods studied for vitamins K and D. The relative standard deviations (RSD) are sufficient for the nutrient levels found in infant formula. RSDs (%) for repeatability (RSDr) and reproducibility (RSDR), respectively, were as follows: iodide, 4.0-11.4 and 13.5-18.2; linoleic acid, 1.0-1.6 and 3.5-5.1; vitamin K1, 3.2-16.0 and 6.2-19.4; and vitamin D3, 4.2 and 35.0. The recommendation to adopt the method for vitamin D was supported by the results of a ministudy. All laboratories were capable of using these methods with little training. The methods for determination of iodide, linoleic acid, and vitamins D and K in ready-to-feed milk-based infant formula have been adopted first action by AOAC International.
authors.affiliation: U.S. Food and Drug Administration, Division of Nutrition, Washington, DC 20204.
authors.forename: J T
authors.initials: JT
authors.lastname: Tanner
authors.forename: S A
authors.initials: SA
authors.lastname: Barnett
authors.forename: M K
authors.initials: MK
authors.lastname: Mountford
mesh_terms: D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D007454:Iodides / Q000032:analysis*; D017736:Ion-Selective Electrodes; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis*; D015203:Reproducibility of Results; D014481:United States; D014486:United States Food and Drug Administration; D014807:Vitamin D / Q000032:analysis*; D014812:Vitamin K / Q000032:analysis*
chemical_list: D007454:Iodides; D008041:Linoleic Acids; D014812:Vitamin K; D014807:Vitamin D; D019787:Linoleic Acid