Influence of cAMP on E-cadherin expression and cell surface heparan sulfate proteoglycan synthesis in human breast cancer cells.
The growth of MCF-7 and MDA-MB-231 human breast cancer cells was inhibited by treatment with dibutyryl cAMP (dBcAMP, 10(-4) M). The effects on E-cadherin expression and cell surface associated heparan sulfate proteoglycans (HSPG) synthesis, both implicated in cell adhesion, were investigated. dBcAMP was demonstrated to increase E-cadherin expression in the E-cadherin positive MCF-7 cells. However, in the E-cadherin negative MDA-MB-231 cells, the treatment did not induce expression of this cell adhesion molecule. Furthermore, in the two cell lines, an increase of the [35S] Na2SO4 incorporation into the cell surface sulfated PG was observed subsequently to dBcAMP treatment. Interestingly, the proportion of cell surface HSPG was also enhanced by this treatment. Taken together, these results demonstrate that the decrease of the proliferation observed in the human breast cancer cells after dBcAMP treatment is associated with an increase in the cell-cell and cell-matrix interactions. This suggests that the metastatic process which involves lack of cohesiveness and migration of the cells may probably be counteracted by cAMP in the human breast cancer cells.
Revillion
F
F
Laboratoire d'Endocrinologie Expérimentale, Centre Oscar Lambret, Lille, France.
Vandewalle
B
B
Hornez
L
L
Lefebvre
J
J
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D003994:Bucladesine / Q000494:pharmacology*; D015820:Cadherins / Q000096:biosynthesis*; D002448:Cell Adhesion; D002455:Cell Division; D005260:Female; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000096:biosynthesis*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011509:Proteoglycans / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D003994:Bucladesine; D015820:Cadherins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D009363:Neoplasm Proteins; D011509:Proteoglycans