Diffusion versus iontophoresis in the transport of gentamicin in the burned rabbit ear model.
This experimental study demonstrates that iontophoresis can be used to transport biologically active gentamicin into auricular cartilage. Fifty female New Zealand White rabbits were divided into three groups: live controls with unburned ears, live animals with burn-injured ears (deep or partial-thickness), and euthanized animals with unburned ears. Each group was then divided into subgroups that received gentamicin transported by iontophoresis or diffusion. In ears subjected to iontophoresis, adequate gentamicin activity could be demonstrated only in the cartilage of euthanized animals with unburned ears and live animals with full-thickness burned ears. Diffusion did not transport a detectable level of gentamicin in any instance. Diffusion is ineffective in the transport of gentamicin and should not be used as a primary method of treatment for ear chondritis. Iontophoresis did not transport active gentamicin across partial-thickness burns, presumably because the migrating antibiotic molecules were dispersed throughout the body by the circulation. However, if the circulation is destroyed, as in a full-thickness burn, iontophoresis can move the biologically active gentamicin into the cartilage.
Kaweski
S
S
Division of Plastic and Reconstructive Surgery, Milton S. Hershey Medical Center, Hershey, PA.
Baldwin
R C
RC
Wong
R K
RK
Manders
E K
EK
D000818:Animals; D001693:Biological Transport, Active; D002056:Burns / Q000378:metabolism*; D004058:Diffusion; D004425:Ear Cartilage / Q000378:metabolism; D004431:Ear, External / Q000378:metabolism*; D005260:Female; D005839:Gentamicins / Q000493:pharmacokinetics*; D007478:Iontophoresis; D008954:Models, Biological; D011817:Rabbits; D013997:Time Factors
D005839:Gentamicins