Localization of the cellular expression pattern of cdc25NEF and ras in the juvenile rat brain.
In this report, we demonstrate that the brain-specific ras nucleotide-exchange factor, cdc25NEF-B, is expressed in specific neuronal populations in the juvenile rat brain. Because cdc25NEF-B likely regulates one or more of the vertebrate ras proteins, H-, K- and N-ras, we also examined their levels of expression and pattern of expression in the juvenile rat brain. We find cdc25NEF-B to be highly expressed in the hippocampus, some deep nuclei, neocortex, and the granule cell layer of the anterior lobules of the cerebellum. Our observations suggest a functional link between cdc25NEF-B and H-ras in a neuronal signal transduction pathway.
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Department of Biochemistry and Molecular Biology, Kenneth Norris Jr. Comprehensive Cancer Center, University of Southern California School of Medicine, Los Angeles 90033.
Schreiber
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Baudry
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Tocco
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Broek
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D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D015870:Gene Expression; D011905:Genes, ras; D006624:Hippocampus / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009857:Oncogenes; D009928:Organ Specificity; D011712:Pyramidal Tracts / Q000378:metabolism; D015347:RNA Probes; D051381:Rats; D017207:Rats, Sprague-Dawley; D013462:Sulfur Radioisotopes
D009419:Nerve Tissue Proteins; D015347:RNA Probes; D013462:Sulfur Radioisotopes