title: Monoclonal antibodies for identification of Borrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis.
abstract: Borrelia isolates associated with Lyme borreliosis were previously divided into 3 genospecies, B. burgdorferi sensu stricto, B. garinii and group VS461, on the basis of DNA homology. B. burgdorferi sensu stricto and B. garinii were identified by monoclonal antibodies (MAbs), H3TS and D6 respectively, but no MAbs were available to identify group VS461. Two MAbs were produced, I 17.3 and J 8.3 which reacted with OspB and OspA proteins, respectively, of strains belonging to group VS461, which should be named B. afzelii sp. nov. 24 strains were assigned to B. afzelii sp. nov., 11 of them being isolated from skin lesions, 6 from acrodermatitis chronica atrophicans (ACA) and 5 from erythema chronicum migrans (ECM). Although quite unknown in the USA, ACA has frequently been reported in northern Europe where B. afzelii sp. nov. is commonly isolated. This study documents the involvement of B. afzelii sp. nov. as a specific aetiological agent of ACA.
authors.affiliation: Unit of Molecular and Medical Bacteriology, Pasteur Institute, Paris, France.
authors.forename: M M
authors.initials: MM
authors.lastname: Canica
authors.forename: F
authors.initials: F
authors.lastname: Nato
authors.forename: L
authors.initials: L
authors.lastname: du Merle
authors.forename: J C
authors.initials: JC
authors.lastname: Mazie
authors.forename: G
authors.initials: G
authors.lastname: Baranton
authors.forename: D
authors.initials: D
authors.lastname: Postic
mesh_terms: D000169:Acrodermatitis / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification; D001898:Borrelia / Q000276:immunology* / Q000302:isolation & purification* / Q000472:pathogenicity; D015787:Erythema Chronicum Migrans / Q000382:microbiology; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology*; D051379:Mice; D017192:Skin Diseases, Bacterial / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins