Distribution of bent DNA structures in the fission yeast centromere.
To gain a clue as to the functional significance of DNA curvature, we experimentally characterized the distribution of bent DNA structures throughout the 35-kb cen1 sequence, one of the isolated functional centromeric DNA of the fission yeast, Schizosaccharomyces pombe. It was revealed that a relatively large central portion of cen1, covering a 2.2-kb DNA sequence, displays a remarkable DNA curvature.
Ueki
N
N
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
Momoi
H
H
Yamada
H
H
Mizuno
T
T
D002503:Centromere / Q000737:chemistry*; D004271:DNA, Fungal / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D009690:Nucleic Acid Conformation; D012568:Schizosaccharomyces / Q000235:genetics*
D004271:DNA, Fungal