Immunoprophylaxis and immunotherapy of respiratory syncytial virus-infected mice with respiratory syncytial virus-specific immune serum.
The effects of passive respiratory syncytial virus (RSV) immune serum given as preinoculation prophylaxis, postinoculation prophylaxis, and as therapy on d 5 after inoculation were evaluated in an illness model of RSV infection in BALB/c mice. Pre- and postinoculation prophylaxis reduced RSV replication in lung after primary RSV infection and prevented illness. Day 5 treatment did not affect peak titer of RSV in lung but resulted in more rapid recovery from illness than seen in untreated mice. Prophylaxis prevented antibody responses and lymphocytic infiltrates in lung after primary RSV infection. Treatment caused diminished antibody and pathologic responses. Prophylaxis increased susceptibility to reinfection, although illness after rechallenge was mild. Mice treated therapeutically were less susceptible to reinfection than mice treated prophylactically, but they also experienced mild illness after rechallenge. Passive antibody prophylaxis and treatment of RSV infection are promising approaches to attenuating lower respiratory tract illness from primary RSV infection. The ability to measure illness endpoints and pathology make the BALB/c mouse model of RSV infection a useful system for the preclinical evaluation of immunoprophylactic and immunotherapeutic modalities.
Graham
B S
BS
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Davis
T H
TH
Tang
Y W
YW
Gruber
W C
WC
D000818:Animals; D000914:Antibodies, Viral / Q000008:administration & dosage / Q000627:therapeutic use; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D005260:Female; D007116:Immunization, Passive; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D018357:Respiratory Syncytial Virus Infections / Q000473:pathology / Q000517:prevention & control* / Q000628:therapy*; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000502:physiology; D014779:Virus Replication
D000914:Antibodies, Viral