Stereospecific actions of misoprostol on rat colonic electrolyte transport.
Misoprostol (Miso) produces a mild, transient diarrhea in some patients, which is believed to be partly due to intraluminal fluid accumulation. To better understand this diarrheagenic action, we compared the effects of Miso, its 4 stereoisomers (11R16R, 11R16S, 11S16S, 11S16R), misoprostol free acid (Miso-FA), and 16,16-dimethyl PGE2 (dmPGE2) on rat colonic electrolyte transport in vitro. Increases in short-circuit current (Isc) were measured (after serosal addition) in segments of mucosa stripped of muscularis and mounted in Ussing chambers. The rank order of apparent potencies, in terms of threshold, were (microM): 11R, 16S (1.2) approximately dmPGE2 (1.0) > Miso-FA (10.0) approximately Miso >> 11R, 16R; 11S, 16R; 11S, 16S (all inactive at 100 microM). The response to dmPGE2 and Miso was attenuated in the presence of the Na+/K+/Cl- co-transport inhibitor bumetanide (100 microM). Pretreatment with atropine (0.1 microM) did not affect the Isc response to Miso, Miso-FA, or dmPGE2. Tetrodotoxin partially attenuated (39 +/- 9% inhibition) the response to Miso-FA, but did not affect Miso or dmPGE2. In conclusion, Miso increases Cl- secretion across rat colonic mucosa through a direct action on epithelial cells. The activity resides in the 11R,16S isomer, thus implying a stereospecific interaction at PGE receptors. The effect of Miso to stimulate epithelial Cl- secretion might contribute to its diarrheagenic action in vivo.
Won-Kim
S
S
Gastrointestinal Diseases Research, Searle Research & Development, Skokie, IL 60077.
Kachur
J F
JF
Gaginella
T S
TS
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004573:Electrolytes / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008297:Male; D016595:Misoprostol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism
D004573:Electrolytes; D016595:Misoprostol; D015064:16,16-Dimethylprostaglandin E2