Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3.
We previously reported a hamster animal model of melanoma in which the tumor tissue expresses gangliosides GM3, GD3, and O-acetyl GD3. This ganglioside pattern is similar to that in human melanomas (Ren, S., A. Slominski, and R. K. Yu. 1989 Cancer Res. 49: 7051). In this study, we isolated and purified these gangliosides using chloroform-methanol extraction, Folch partition, chromatographies on DEAE-Sephadex A-25, and Iatrobeads columns. The yields of gangliosides GM3, GD3, and O-acetyl GD3 were 44.1 mg, 19.6 mg, and 9 mg per 100 g of Ma melanotic melanoma tissues, respectively. The structures of these gangliosides were characterized by periodate oxidation, gas chromatographic (GC) analysis, fast-atom bombardment-mass spectrometry (FAB-MS), and nuclear magnetic resonance (NMR) studies. The structure of hamster melanoma O-acetyl GD3 is different from the 9-O-acetyl GD3 previously reported in human melanoma. The major fatty acids of this ganglioside are C16:0, C18:0, C20:0, C22:0, and C24:0 and the long-chain base is C18-sphingosine.
Ren
S
S
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Ariga
T
T
Scarsdale
J N
JN
Zhang
Y
Y
Slominski
A
A
Livingston
P O
PO
Ritter
G
G
Kushi
Y
Y
Yu
R K
RK
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000302:isolation & purification; D006224:Cricetinae; D005732:Gangliosides / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008546:Melanoma, Experimental / Q000276:immunology*; D015394:Molecular Structure; D010504:Periodic Acid / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000951:Antigens, Neoplasm; D005732:Gangliosides; D010504:Periodic Acid; C049132:9-O-acetyl-GD3 ganglioside; C009288:metaperiodate