Characterisation, cloning and integrative properties of the gene encoding urate oxidase in Aspergillus nidulans.
A number of mutations have been obtained which define the structural gene (uaZ) coding for urate oxidase in linkage group I of Aspergillus nidulans. This gene has been cloned by transformation of a uaZ- null mutant. A chromosome I/VIII translocation which splits the gene has been defined both genetically and physically. All known mutations are contained in a 1-kb fragment, itself contained in the probe which recognizes a 1.2-kb inducible message. Plasmids carrying uaZ show a strict bias towards homologous recombination in transformation experiments.
Oestreicher
N
N
Institut de Génétique et Microbiologie, Unité Associée au CNRS 1354, Université Paris-Sud, Centre d'Orsay, France.
Sealy-Lewis
H M
HM
Scazzocchio
C
C
D000483:Alleles; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005800:Genes, Fungal; D010957:Plasmids; D011233:Precipitin Tests; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014170:Transformation, Genetic; D014503:Urate Oxidase / Q000235:genetics* / Q000378:metabolism
D014503:Urate Oxidase