The chronic myelocytic cell line K 562 contains minor (m) as well as major (M) ber/abl fusion mRNAs.
By searching for additional chimeric bcr/abl transcripts in K 562 cells characterized by major (M) bcr/abl fusions, a new mRNA, a minor (m) bcr/abl transcript, was detected. A practical implication of this finding is that the K 562 cell line can be used as positive control for the detection by the polymerase chain reaction of both types of transcripts for the diagnosis of Philadelphia chromosome associated leukemias.
Seelig
R
R
Institute of Immunology and Molecular Genetics, Karlsruhe, Germany.
Schranz
P
P
Bottner
C
C
Neubauer
A
A
Seelig
H P
HP
Renz
M
M
D001483:Base Sequence; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000737:chemistry
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016044:Fusion Proteins, bcr-abl