The interaction of 2,4-dichlorophenoxyacetic acid, ribosomes and polyamines in Azospirillum brasilense.
2,4-Dichlorophenoxyacetic acid (2,4-D) is an herbicide used extensively in agriculture. We had previously determined that 1 mM 2,4-D could inhibit cell growth, DNA and protein synthesis of Azospirillum brasilense. The present work was designed to determine if these alterations are a consequence of 2,4-D action on polyamine biosynthesis and if the protein synthesis inhibition is a result of ribosomal impairment. In this paper we demonstrate that 2,4-D alters the metabolism of polyamines and, thus, affects protein synthesis at the ribosomal level.
Fabra
A
A
Facultad de Ciencias Exactas, Universidad Nacional de Rio Cuarto, Córdoba, Argentina.
Giordano
W
W
Rivarola
V
V
Mori
G
G
Castro
S
S
Balegno
H
H
D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity*; D015806:Azospirillum brasilense / Q000187:drug effects* / Q000378:metabolism; D015317:Biogenic Polyamines / Q000096:biosynthesis* / Q000494:pharmacology; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism
D015317:Biogenic Polyamines; D015084:2,4-Dichlorophenoxyacetic Acid