Combined enzymic and chromatographic techniques to determine specific radioactivity in free and triglyceride fatty acid plasma fractions.
A reliable method to determine both free and triglyceride fatty acids and simultaneously to determine the specific radioactivity in each fraction has been developed. The procedure can be used to analyse a large number of samples. Lipoprotein lipase was used to hydrolyse triglyceride fatty acids, and a Carbopack B column was used to isolate free fatty acids. The radiolabeled fatty acids were determined by liquid scintillation counting, and individual fatty acid levels in each fraction were determined by gas chromatography. Free and triglyceride fatty acids were eluted in different fractions from the Carbopack B column. No interferences from other compounds were significant.
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Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears i Institut d'Estudis Avançats (UIBCSIC), Palma de Mallorca, Spain.
Palou
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Pons
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002845:Chromatography; D002849:Chromatography, Gas; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D006868:Hydrolysis; D007202:Indicators and Reagents; D008071:Lipoprotein Lipase; D009829:Oleic Acids / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013395:Sucrose / Q000032:analysis; D014280:Triglycerides / Q000097:blood*
D000596:Amino Acids; D005230:Fatty Acids, Nonesterified; D007202:Indicators and Reagents; D009829:Oleic Acids; D014280:Triglycerides; D013395:Sucrose; D008071:Lipoprotein Lipase