Monoclonal anti-idiotypic antibodies against human anti-thyroglobulin autoantibodies recognize idiotopes shared by disease-associated and natural anti-thyroglobulin autoantibodies.
Monoclonal murine anti-idiotypic antibodies generated against purified human anti-thyroglobulin (TG) antibodies from a patient with Hashimoto's thyroiditis recognized two major clusters of idiotopes (1C2 and 1B1) on human anti-thyroglobulin antibodies which were framework and paratope-related. A survey of affinity-purified autoantibodies from Hashimoto's patients indicated that the 1C2 and 1B1 idiotopes are expressed on the disease-associated anti-TG antibodies from several patients. 1C2 and 1B1 idiotopes are also detected on affinity-purified anti-TG antibodies isolated from serum of healthy donors and with therapeutic preparations of immunoglobulins obtained from plasma pools of healthy individuals. The observation that both framework and paratope-related idiotopes are shared by natural and patients' autoantibodies suggests that the anti-TG autoantibodies found in sera of patients with Hashimoto's disease represent an abnormal expansion of normal autoreactive B cell clones rather than the emergency of an antigen-driven, disease-specific B cell response.
Kaveri
S V
SV
Markey Cancer Center, University of Kentucky, Lexington 40536-0093.
Wang
H
H
Rowen
D
D
Kazatchkine
M D
MD
Kohler
H
H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D015551:Autoimmunity / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D016756:Immunoglobulins, Intravenous / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D013954:Thyroglobulin / Q000276:immunology*; D013966:Thyroiditis / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D016756:Immunoglobulins, Intravenous; D013954:Thyroglobulin