title: 32P-postlabelling analysis of alkylphosphotriesters in DNA.
abstract: We have developed a highly sensitive 32P-postlabelling assay for the detection of alkylphosphotriesters in DNA using selective enzymatic DNA hydrolysis, sample preparation by C18 Sep-Pak solid phase extraction and product analysis by HPLC. The assay provides information on the quantity of the alkylphosphotriesters and gives some clues with respect to their identity.
authors.affiliation: Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam.
authors.forename: C P
authors.initials: CP
authors.lastname: Saris
authors.forename: S J
authors.initials: SJ
authors.lastname: Damman
authors.forename: A M
authors.initials: AM
authors.lastname: van den Ende
authors.forename: J G
authors.initials: JG
authors.lastname: Westra
authors.forename: L
authors.initials: L
authors.lastname: den Engelse
mesh_terms: D000478:Alkylation; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004249:DNA Damage; D005069:Evaluation Studies as Topic; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D008722:Methods; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D051381:Rats
chemical_list: D010761:Phosphorus Radioisotopes; D004247:DNA