title: Vulnerability and adaptation of distortion product otoacoustic emissions to endocochlear potential variation.
abstract: The endocochlear potential (EP) was reversibly decreased in adult gerbils by the intraperitoneal injection of furosemide, while cochlear functioning was monitored by measurement of distortion production otoacoustic emissions (DPE) at a range of stimulus intensities. Stimulus frequencies for DPEs were f1 = 6.8 and f2 = 8 kHz (f2/f1 = 1.18). Emissions monitored in the ear canal and scala media were 2f1-f2, 3f1-2f2, 2f2-f1, and f2-f1. Typically, the EP decreased smoothly, reached a minimum one-half hour after injection, then recovered slowly over several hours. Emissions at 2f1-f2 and 3f1-2f2 at low stimulus levels were particularly vulnerable to the change in EP. These vulnerable emissions showed characteristic trajectories in which the amplitudes changed little with the initial EP decrease, then dropped sharply as the EP continued to decrease. However, the amplitudes then began to recover even before the EP reached minimum, and recovered completely while the EP remained subnormal. The trajectories of the other odd order emissions were similar, but lacked the abrupt decrease. The variation of the even order (f2-f1) component was completely different, but appeared related to the odd order trajectories in a complex fashion. During the initial decrease for the vulnerable components, the decrease in emission amplitude (in dB) was found to be proportional to the square of the change in EP (in mV). The recovery with a subnormal EP was interpreted as an adaptive effect with a time constant of about 15 min.
authors.affiliation: Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology, Head and Neck Surgery, University of Washington, Seattle 98195.
authors.forename: D M
authors.initials: DM
authors.lastname: Mills
authors.forename: S J
authors.initials: SJ
authors.lastname: Norton
authors.forename: E W
authors.initials: EW
authors.lastname: Rubel
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D003051:Cochlea / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D003053:Cochlear Duct / Q000502:physiology; D004431:Ear, External; D005665:Furosemide; D005849:Gerbillinae; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
chemical_list: D005665:Furosemide