title: [The worsening of tissue damage due to the transient release during the ischemia of rat small intestine--the relation with reactive oxygen species and lipid peroxidation].
abstract: Ischemia and reperfusion experiments were carried out using rat small intestine. Intestinal segment and supplying vessels was occluded and released after following procedure: In group I, 60 minute occlusion. In group II, 30 minute occlusion-10 minute release-20 minute occlusion. In group III, superoxide dismutase (SOD) and catalase (CAT) were given during the period of intermittent release in the same time schedule as in group II. Tissue damage was evaluated histopathologically and lipid peroxide (LPO) of involved segment and chemiluminescence (CL) of draining venous blood was investigated. It was concluded as follows: 1. In these experiments, the tissue damage and the increment of LPO and CL were severer in group II than in group I. Then, the reactive oxygen species (ROS) derived from neutrophils was thought to play an important role in the tissue damage. 2. At the end of intermittent release, LPO and CL increased in group II, and these increments were suppressed by SOD and CAT treatment in group III. 3. After last release, the tissue damage in group II was diminished in group III. The severe tissue damage after intermittent occlusion is thought to be caused by reperfusion injury during the intermittent release.
authors.affiliation: Second Department of Surgery, Yokohama City University, School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Moriwaki
authors.forename: H
authors.initials: H
authors.lastname: Katamura
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: M
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D002374:Catalase / Q000008:administration & dosage; D007421:Intestine, Small / Q000098:blood supply*; D007511:Ischemia / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000008:administration & dosage
chemical_list: D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase