Mechanism of inhibition of DNA synthesis by 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil.
The mechanism of the inhibitory action of 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl) uracil triphosphate (BV-araUTP) on DNA synthesis by Escherichia coli DNA polymerase I Klenow fragment was studied. Acting as a chain terminator, BV-araUTP inhibited DNA synthesis by Klenow fragment more effectively than 2',3'-dideoxythymidine triphosphate (ddTTP). However, the incorporation sites of BV-araU monophosphate were restricted at consecutive dTMP sequence whereas ddTMP was incorporated at every dTMP site.
Suzutani
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T
Division of Virology, National Institute for Medical Research, Mill Hill, London, U.K.
Machida
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H
Honess
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RW
D000998:Antiviral Agents / Q000494:pharmacology*; D001086:Arabinofuranosyluracil / Q000031:analogs & derivatives* / Q000494:pharmacology; D004256:DNA Polymerase I / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis* / Q000187:drug effects*; D054306:Dideoxynucleotides; D004926:Escherichia coli / Q000235:genetics*; D013942:Thymine Nucleotides / Q000494:pharmacology
D000998:Antiviral Agents; D004269:DNA, Bacterial; D054306:Dideoxynucleotides; D013942:Thymine Nucleotides; D001086:Arabinofuranosyluracil; C031368:sorivudine; D004256:DNA Polymerase I; C017216:2',3'-dideoxythymidine triphosphate