A simple protocol for the physical cleavage of Trypanosoma cruzi kinetoplast DNA present in blood samples and its use in polymerase chain reaction (PCR)-based diagnosis of chronic Chagas disease.
Britto
C
C
Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Cardoso
M A
MA
Wincker
P
P
Morel
C M
CM
D000818:Animals; D014355:Chagas Disease / Q000175:diagnosis*; D018105:DNA, Kinetoplast / Q000032:analysis*; D006358:Hot Temperature; D016133:Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics*
D018105:DNA, Kinetoplast