Optimization of RNA and DNA purification procedures from human colorectal carcinomas.
We optimized procedures to extract RNA and DNA separately from colorectal carcinomas pulverized by Microdismembrator II. Our procedures make it possible to achieve good yields and high degree of purification of both types of macromolecules without affecting their structural integrity.
La Farina
M
M
Dipartimento di Biologia Cellulare e dello Sviluppo, University of Palermo, Italy.
Alberti
M
M
Russo
A
A
Bazan
V
V
Grasso
G
G
Romancino
D
D
Bazan
P
P
Albanese
I
I
D015179:Colorectal Neoplasms / Q000737:chemistry*; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D004586:Electrophoresis; D006801:Humans; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000302:isolation & purification*
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm