Equilibrium and kinetic studies of the interaction of the benzimidazole dye Hoechst 8208 with nucleic acids.
The interaction of the benzimidazole dye Hoechst 8208 (H8208) with nucleic acids has been studied by temperature-jump relaxation method. The dependence of relaxation times on the nucleic acid concentration indicates that H8208 binds to RNA by a single biomolecular reaction and to DNA by a bimolecular reaction, followed by a slow isomerization.
Hasegawa
N
N
Department of Chemistry, Faculty of Science, Yamaguchi University, Japan.
Kubota
Y
Y
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry*; D002417:Cattle; D004396:Coloring Agents / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D007700:Kinetics; D009696:Nucleic Acids / Q000737:chemistry*
D000814:Aniline Compounds; D001562:Benzimidazoles; D004396:Coloring Agents; D007364:Intercalating Agents; D009696:Nucleic Acids; C023989:H8208