Infrared CD of deoxy oligonucleotides. Conformational studies of 5'd(GCGC)3', 5'd(CGCG)3', 5'd(CCGG)3', and 5'd(GGCC)3' in low and high salt aqueous solution.
Infrared (vibrational) circular dichroism (VCD) spectra have been obtained for the self-complementary tetranucleotides, 5'd(CGCG)3', 5'd(GCGC)3', 5'd(CCGG)3', and 5'd(GGCC)3'. In buffered aqueous solution at low salt concentration, these tetramers exhibit spectra associated with right-handed polymers, although the spectra differ significantly for the four species. In high salt solution, a B-->Z transition occurs in 5'd(CGCG)3', while the other tetranucleotides appear only slightly altered. Temperature dependent studies of these oligonucleotides reveal a greater amount of thermal stability for the tetramers in low salt than for the high salt solutions. VCD intensities computed via the exciton formalism are compared with observed results.
Birke
S S
SS
Department of Chemistry and Biochemistry, City University of New York, Hunter College, New York 10021.
Moses
M
M
Kagalovsky
B
B
Jano
D
D
Gulotta
M
M
Diem
M
M
D001483:Base Sequence; D055592:Biophysical Phenomena; D001703:Biophysics; D002942:Circular Dichroism; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009994:Osmolar Concentration; D012492:Salts; D012996:Solutions; D013055:Spectrophotometry, Infrared; D013696:Temperature; D013816:Thermodynamics; D014867:Water
D009838:Oligodeoxyribonucleotides; D012492:Salts; D012996:Solutions; D014867:Water