Time course changes in cytoskeletal structures of cultured endothelial cells exposed to shear stress.
Changes in patterns of microfilament distribution were analyzed using cultured porcine aortic endothelial cells under shear flow. Within the elongated endothelial cells after shear flow exposure (20 dyne/cm2, 0-24 h), F-actin filaments were mainly observed as stress fibers. After 24 h exposure, the fluorescence intensity of rhodamine-phalloidin-stained cells was enhanced, indicating the increase in F-actin content. It was suggested that not only F-actin distribution but also the content may be affected by shear stress at the early stage of exposure.
Ookawa
K
K
Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Sato
M
M
Ohshima
N
N
D008841:Actin Cytoskeleton / Q000473:pathology; D000199:Actins / Q000032:analysis; D000818:Animals; D001012:Aorta, Abdominal / Q000737:chemistry / Q000473:pathology; D001013:Aorta, Thoracic / Q000737:chemistry / Q000473:pathology; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000032:analysis; D003599:Cytoskeleton / Q000473:pathology*; D004730:Endothelium, Vascular / Q000737:chemistry / Q000473:pathology*; D008856:Microscopy, Fluorescence; D010784:Photomicrography; D012212:Rheology; D013314:Stress, Mechanical; D013552:Swine; D013997:Time Factors
D000199:Actins; D003598:Cytoskeletal Proteins