A pulsed laser and pulse radiolysis study of amphiphilic chlorophyll derivatives with PDT activity toward malignant melanoma.
Two amphiphilic derivatives of chlorophyll, which have high potential as photodynamic therapy sensitizers for malignant melanoma have been investigated by a combination of laser flash photolysis and pulse radiolysis. It is shown that direct excitation of monomeric forms of these molecules in both hydrophilic and hydrophobic environments produces significant yields of the corresponding triplet states, which have been characterized in terms of spectral and kinetic parameters. In both environments, scavenging of the triplets by oxygen produces singlet oxygen, O2(1 delta g), with essentially unit efficiency as evidenced by time-resolved IR luminescence measurements.
Fiedor
L
L
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Gorman
A A
AA
Hamblett
I
I
Rosenbach-Belkin
V
V
Salomon
Y
Y
Scherz
A
A
Tregub
I
I
D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000528:radiation effects / Q000627:therapeutic use; D006801:Humans; D007834:Lasers; D008545:Melanoma / Q000188:drug therapy*; D010100:Oxygen; D010777:Photochemistry; D010778:Photochemotherapy; D010782:Photolysis; D011838:Radiation-Sensitizing Agents / Q000737:chemistry*; D026082:Singlet Oxygen; D013053:Spectrophotometry
D011838:Radiation-Sensitizing Agents; D002734:Chlorophyll; D026082:Singlet Oxygen; D010100:Oxygen