Association of nucleosides and their 5'-monophosphates with a tryptophan containing tripeptide, Trp-Leu-Glu: the source of an overestimation by fluorescence spectroscopy.
Association of 7-methylguanosine 5'-monophosphate with a tryptophan containing tripeptide, Trp-Leu-Glu, has been studied by fluorescence titration using two different geometries of detection, viz. right angle and front surface geometry. The applicability of these two techniques to determine the stability constant of the nucleotide-peptide adduct is discussed. Evidence is presented that fluorescence titration based on right angle detection may lead to considerable overestimation of the strength of interaction.
Wieczorek
Z
Z
Department of Physics and Biophysics, University of Agriculture and Technology, Olsztyn, Poland.
Stepinski
J
J
Darzynkiewicz
E
E
Lönnberg
H
H
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D009705:Nucleosides / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D012314:RNA Cap Analogs / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry*
D009705:Nucleosides; D009842:Oligopeptides; D012314:RNA Cap Analogs; C011925:7-methylguanosine-5'-monophosphate; C056552:tryptophyl-leucyl-glutamic acid; D014364:Tryptophan