title: Determination of the urinary D/L trans-3-hydroxyproline ratio: a noninvasive screening test for Alport syndrome.
abstract: Because disturbed conformation of connective tissue proteins can be accompanied by increased racemization (i.e., an increased ratio of dextrorotatory (D) to levorotatory (L) amino acid molecules), we studied by high-performance liquid chromatography the renal excretion of the D-form of the basement membrane-specific trans-3-hydroxyproline in patients with Alport syndrome. The D/L ratio was significantly higher in patients with Alport syndrome than in patients with other renal diseases or in healthy control subjects. We therefore suggest that this factor may be a simple noninvasive (screening) test for Alport syndrome.
authors.affiliation: Department of Pediatrics, University of Vienna, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Lubec
authors.forename: K
authors.initials: K
authors.lastname: Arbeiter
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000652:urine; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine*; D007223:Infant; D007231:Infant, Newborn; D007536:Isomerism; D008875:Middle Aged; D009394:Nephritis, Hereditary / Q000175:diagnosis* / Q000652:urine*
chemical_list: D015415:Biomarkers; C026215:3-hydroxyproline; D006909:Hydroxyproline