title: Vitamin D3 (soltriol) nuclear receptors in abdominal scent gland and skin of Siberian hamster (Phodopus sungorus) localized by autoradiography and immunohistochemistry.
abstract: In vivo autoradiography with [3H]1,25-dihydroxycholecalciferol (vitamin D, soltriol) and immunostaining with antibodies to vitamin D receptor were applied to identify specific binding sites in the abdominal scent gland of male Siberian hamster (Phodopus sungorus). Nuclear concentration of radiolabeled hormone and receptor antibodies was observed in the corresponding cell types including basal cells of sebaceous glands, cells of the outer hair sheaths and hair bulbs, and also keratinocytes in the epidermis. Cells of the hair dermal papillae and fibroblasts of the dermis did not show nuclear labeling. There was good correspondence between the autoradiographic and immunohistochemical data. The results indicate the presence of receptors for vitamin D-soltriol and suggest a seasonal regulation of scent gland marking activities by this steroid hormone of sunlight in cooperation with the sex steroid testosterone.
authors.affiliation: Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599-7090.
authors.forename: W E
authors.initials: WE
authors.lastname: Stumpf
authors.forename: M M
authors.initials: MM
authors.lastname: Pérez-Delgado
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: H J
authors.initials: HJ
authors.lastname: Bidmon
authors.forename: P
authors.initials: P
authors.lastname: Tuohimaa
mesh_terms: D000818:Animals; D001345:Autoradiography; D002117:Calcitriol / Q000276:immunology / Q000378:metabolism*; D006224:Cricetinae; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000648:ultrastructure; D008297:Male; D017111:Phodopus; D018167:Receptors, Calcitriol / Q000276:immunology / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000276:immunology / Q000378:metabolism*; D012543:Scent Glands / Q000378:metabolism* / Q000648:ultrastructure; D012867:Skin / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D018167:Receptors, Calcitriol; D018160:Receptors, Cytoplasmic and Nuclear; D002117:Calcitriol