Cloning and sequence analysis of a full length cDNA encoding human mitochondrial 3-oxoacyl-CoA thiolase.
The cDNA sequence of human mitochondrial 3-oxoacyl-CoA thiolase was determined. The nucleotide sequence contains an open reading frame of 1191 base pairs and encodes an amino acid sequence of 397 residues which exhibits 86.6% homology with that of the rat enzyme. Northern blot analysis gave a single mRNA species of 1.6 kb in the human liver, fibroblasts and intercostal muscle.
Abe
H
H
Department of Pediatrics, Chiba University School of Medicine, Japan.
Ohtake
A
A
Yamamoto
S
S
Satoh
Y
Y
Takayanagi
M
M
Amaya
Y
Y
Takiguchi
M
M
Sakuraba
H
H
Suzuki
Y
Y
Mori
M
M
D000102:Acetyl-CoA C-Acyltransferase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D012333:RNA, Messenger; D000102:Acetyl-CoA C-Acyltransferase