title: Pharmacological characteristics of tachykinin receptors mediating acetylcholine release from neonatal rat spinal cord.
abstract: The pharmacological profiles of tachykinin receptors mediating the release of acetylcholine were examined in the isolated spinal cord of the neonatal rat. The acetylcholine release evoked by neurokinin A or acetyl-[Arg6,Pro9]substance P-(6-11) was depressed by the tachykinin antagonists, spantide and GR71251 at 10 microM, whereas the release evoked by substance P (0.3 microM) or neurokinin B (0.3 microM) was not affected by these tachykinin antagonists. Polymerase chain reaction amplification of rat spinal cord cDNA and sequence analysis revealed the presence of a substantial amount of fragments having sequences identical to that of the NK1 or NK3 receptor, but only a few having a sequence identical to that of the NK2 receptor. These results suggest that in the neonatal rat spinal cord a novel subtype of tachykinin receptor similar but not identical to the classical NK1 receptor is involved in tachykinin-evoked acetylcholine release.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Yoshioka
authors.forename: T
authors.initials: T
authors.lastname: Maehara
authors.forename: R M
authors.initials: RM
authors.lastname: Hagan
authors.forename: S
authors.initials: S
authors.lastname: Nakanishi
authors.forename: M
authors.initials: M
authors.lastname: Otsuka
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000187:drug effects; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000187:drug effects; D018039:Receptors, Tachykinin / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D015320:Tachykinins / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D009467:Neuromuscular Depolarizing Agents; D018040:Receptors, Neurokinin-1; D018039:Receptors, Tachykinin; D015320:Tachykinins; D000109:Acetylcholine