title: Myocardial metabolism in chronic reperfusion after nontransmural infarction in pig hearts.
abstract: The purpose of these studies was to evaluate metabolic behavior in a 4-day reperfusion model in pigs after induction of subendocardial infarction. Two groups of swine [sham and intervention (Int) groups, n = 7) and 10 hearts per group, respectively] were prepared comparably with two surgical procedures separated over 4 days. In the Int group at the time of the first surgery, coronary flow in the left anterior descending (LAD) circulation was partially restricted (by 60%) for 60 min and was then reperfused. LAD myocardium at the time of the second surgery in both groups was extracorporeally perfused aerobically (5.9 +/- 0.2 ml.min-1.g dry wt-1) for 60 min and infused by equilibrium labeling with [U-14C]-palmitate and [5-3H]glucose to estimate fatty acid oxidation and exogenous glucose utilization. During extracorporeal perfusion, regional myocardial shortening and oxygen consumption were comparable between groups despite a marginal impairment in ATP resynthesis by mitochondria (26% decrease, P < 0.071) in Int hearts and a significant decline in mitochondrial respiration (45% decrease in respiratory control rate, P < 0.008; and 41% decrease in state 3 respiration, P < 0.032) as compared with sham hearts. Fatty acid oxidation described by 14CO2 production was 34.00 +/- 4.72 mumol.h-1.g dry wt-1 (averaged from 30-60 min of perfusion) in sham hearts but was decreased (by 48%, P < 0.004) in Int hearts. This reduction in fatty acid utilization may in part be explained by declines in the observed activity of the mitochondrial membrane transporter enzyme, carnitine palmitoyltransferase.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Cardiology Section, University of Wisconsin Hospital and Clinics, Madison 53792-3248.
authors.forename: A J
authors.initials: AJ
authors.lastname: Liedtke
authors.forename: B
authors.initials: B
authors.lastname: Renstrom
authors.forename: S H
authors.initials: SH
authors.lastname: Nellis
authors.forename: R
authors.initials: R
authors.lastname: Subramanian
authors.forename: G
authors.initials: G
authors.lastname: Woldegiorgis
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D003331:Coronary Vessels / Q000502:physiology / Q000503:physiopathology; D005947:Glucose / Q000378:metabolism*; D006321:Heart / Q000502:physiology / Q000503:physiopathology; D006339:Heart Rate; D006439:Hemodynamics; D008929:Mitochondria, Heart / Q000378:metabolism*; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D012016:Reference Values; D013552:Swine; D013599:Systole; D014316:Tritium; D016277:Ventricular Function, Left
chemical_list: D002250:Carbon Radioisotopes; D010169:Palmitic Acids; D014316:Tritium; D019308:Palmitic Acid; D005947:Glucose