Immunoassay reagents for psychoactive drugs. Part 3. Removal of phenothiazine interferences in the quantification of tricyclic antidepressants.
Phenothiazines and their metabolites are known to interfere in the quantification of tricyclic antidepressants (TCAs). A method for selective chemical modification of phenothiazines by chloramine-T in the presence of TCAs is described. This method allows for accurate quantification of the TCA analyte in a serum sample without interference from the modified phenothiazine.
Adamczyk
M
M
Abbott Laboratories D-9NM, Abbott Park, IL 60064.
Fishpaugh
J R
JR
Harrington
C A
CA
Hartter
D E
DE
Hruska
R E
RE
Vanderbilt
A S
AS
D000929:Antidepressive Agents, Tricyclic / Q000032:analysis / Q000097:blood*; D002700:Chloramines; D002746:Chlorpromazine / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay; D008954:Models, Biological; D010084:Oxidation-Reduction; D010640:Phenothiazines / Q000032:analysis / Q000097:blood*; D011619:Psychotropic Drugs / Q000032:analysis / Q000097:blood*; D014105:Tosyl Compounds
D000929:Antidepressive Agents, Tricyclic; D002700:Chloramines; D010640:Phenothiazines; D011619:Psychotropic Drugs; D014105:Tosyl Compounds; C016300:chloramine-T; C031637:phenothiazine; D002746:Chlorpromazine