Measurements and analyses of electrophoretic mobilities of RAW117 lymphosarcoma cells and their variant cells.
The electrophoretic mobilities of the cells of malignant lymphosarcoma cell line RAW117-P and its variant H10 with a highly metastatic property to the liver have been measured at various ionic strengths. The cells of parental cell line (RAW117-P) show higher mobility values in magnitude than those of its variant line (RAW117-H10) in the whole range of electrolyte concentration measured. We have also measured the sialic acid amount carried by cells of both lines. The content of sialic acids in RAW117-H10 cells is observed to be about 27% less than that in RAW117-P cells. The mobility data obtained have been analyzed by a novel mobility formula for colloidal particles with ion-penetrable surface charge layers. The observed mobility difference between RAW117-P cells and RAW117-H10 cells is found to be due to the difference in friction exerted by the cell surface layers on the liquid flow around the cells between these two types of cells and to the difference in fixed-charge density in their surface layers, which is caused by the 27% decrease in sialic acid content. A possible explanation for this mobility difference between these two types of cells is given.
Makino
K
K
Faculty of Pharmaceutical Science, Science University of Tokyo, Japan.
Taki
T
T
Ogura
M
M
Handa
S
S
Nakajima
M
M
Kondo
T
T
Ohshima
H
H
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry*; D051379:Mice; D019158:N-Acetylneuraminic Acid; D009994:Osmolar Concentration; D012794:Sialic Acids / Q000032:analysis; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid