[Effect of quercetin on chemiluminescence of human platelets induced by arachidonic acid].
Arachidonic acid (AA)-induced platelet chemiluminescence (CL) was measured with a lumiphotometer. Quercetin remarkably inhibited the CL, the IC50 of quercetin was 3 mumol.L-1. When quercetin plus aspirin, which inhibits only cyclooxygenase, was added, the inhibitory rate of platelet-CL obviously increased (P < 0.01). On the other hand, the quercetin had a scavenging effect on superoxide anion radical using alkaline sodium dithionite solution generation. The IC50 was 20.9 mumol.L-1. In addition, superoxide dismutase of 0.1 mg.ml-1 inhibited the platelet-CL by 97.8%, while mannitol, a hydroxyl radical scavenger, only by 43.3% at a concentration of 80 mg.ml-1. These results suggest that the mechanism of the inhibiting AA-induced platelet-CL by quercetin was associated with scavenging the superoxide anion radical directly and with inhibiting the cyclooxygenase.
Gu
Z L
ZL
Department of Pharmacology, Suzhou Medical College, China.
Xie
M L
ML
Qian
Z N
ZN
D016718:Arachidonic Acid / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects*; D004357:Drug Synergism; D016166:Free Radical Scavengers; D006801:Humans; D008163:Luminescent Measurements; D008353:Mannitol / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology
D016166:Free Radical Scavengers; D016718:Arachidonic Acid; D008353:Mannitol; D011794:Quercetin; D013482:Superoxide Dismutase; D001241:Aspirin