Differentiation of HL60 promyelocytic cells is promoted by a 'differentiation enhancing factor' produced by erythroleukemia cells.
A differentiation enhancing factor isolated from murine erythroleukemia cells is also a potent enhancer of the differentiation of HL60 human promyelocytic leukemia cells, induced by retinoic acid and by phorbol ester. This stimulating effect is the result of a large increase in the sensitivity of HL60 cells for retinoic acid and for phorbol 12-myristate 13-acetate (20-fold and 40-fold, respectively). Accelerated differentiation induced by the protein factor, and monitored by the appearance of marker enzymes, is accompanied by a large increase in the fluctuation of the levels of protein kinase C (PKC) isozymes in HL60 cells. These results provide further support for the role of this new protein factor in cell differentiation and indicate that other cell types are susceptible to its biological effect.
Sparatore
B
B
Institute of Biological Chemistry, University of Genoa, Italy.
Passalacqua
M
M
Patrone
M
M
Pessino
A
A
Melloni
E
E
Pontremoli
S
S
D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D024243:HMGB1 Protein; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D015473:Leukemia, Promyelocytic, Acute; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D063086:Organothiophosphates / Q000378:metabolism / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D024243:HMGB1 Protein; D007527:Isoenzymes; D009363:Neoplasm Proteins; D063086:Organothiophosphates; C006863:butyl phosphorotrithioate; D014212:Tretinoin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate