title: Involvement of the central nervous system in the salivary secretion induced by pilocarpine in rats.
abstract: The effect of rats of an anteroventral third ventricle (AV3V) electrolytic lesion on salivary secretion induced by intraperitoneal (i.p.) or intracerebroventricular (i.c.v.) injection of a cholinergic agonist (pilocarpine) was investigated. Sham- or AV3V-lesioned rats anesthetized with urethane and with a stainless steel cannula implanted into the lateral ventricle (LV) were used. The amount of salivary secretion was studied over a seven-minute period after i.c.v. or i.p. injection of pilocarpine. In sham-operated rats, i.p. injection of pilocarpine (1 mg/kg b.w.) (after 6 h, 2, 7, and 15 days) produced salivary secretion (486 +/- 21, 778 +/- 85, 630 +/- 50, and 560 +/- 55 mg/7 min, respectively). This effect was reduced 6 h, 2, and 7 days after an AV3V lesion (142 +/- 22, 113 +/- 32, and 290 +/- 62 mg/7 min, respectively), but not 15 days after an AV3V lesion (516 +/- 19 mg/7 min). I.c.v. injection of pilocarpine (120 micrograms in 1 microL), in sham-operated rats after 6 h, 2, 7, and 15 days also produced salivary secretion (443 +/- 20, 417 +/- 81, 496 +/- 14, and 427 +/- 47 mg/7 min, respectively). The effects of i.c.v. pilocarpine were also reduced 6 h, 2, and 7 days after an AV3V lesion (143 +/- 19, 273 +/- 14, and 322 +/- 17 mg/7 min, respectively), but not after 15 days (450 +/- 28 mg/7 min). The results demonstrate that the central nervous system, and particularly the AV3V region, is important for the effect of pilocarpine on salivary secretion in rats.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, School of Dentistry, Paulista State University, SP, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Renzi
authors.forename: E
authors.initials: E
authors.lastname: Colombari
authors.forename: T R
authors.initials: TR
authors.lastname: Mattos Filho
authors.forename: J E
authors.initials: JE
authors.lastname: Silveira
authors.forename: W A
authors.initials: WA
authors.lastname: Saad
authors.forename: L A
authors.initials: LA
authors.lastname: Camargo
authors.forename: L A
authors.initials: LA
authors.lastname: de Luca Júnior
authors.forename: J G
authors.initials: JG
authors.lastname: Deróbio
authors.forename: J V
authors.initials: JV
authors.lastname: Menani
mesh_terms: D000818:Animals; D001927:Brain Diseases / Q000473:pathology / Q000503:physiopathology; D002552:Cerebral Ventricles / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D007267:Injections; D007274:Injections, Intraperitoneal; D008297:Male; D009434:Neural Pathways / Q000187:drug effects / Q000502:physiology; D010862:Pilocarpine / Q000008:administration & dosage / Q000494:pharmacology*; D016548:Prosencephalon / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D051381:Rats; D012463:Saliva / Q000187:drug effects* / Q000378:metabolism*; D012469:Salivary Glands / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D012636:Secretory Rate / Q000187:drug effects; D013997:Time Factors
chemical_list: D010862:Pilocarpine