Comparison of the functional activities of two different preparations of human recombinant tumor necrosis factor alpha.
The role of tumor necrosis factor (TNF-alpha) in physiological and pathological reactions has resulted in a progressive increase of expensive TNF-alpha consumption for laboratory and clinical purposes. Following this trend, the first chemical synthesis of the gene for rHuTNF-alpha gene in Poland and its subsequent successful expression in E. coli was recently reported. In the present paper, we verify the in vitro biological activities of this TNF-alpha preparation (CMMS/TNF-alpha) in comparison with a commercially available preparation of TNF-alpha. We demonstrate that our TNF-alpha possesses strong cytotoxic activity against WEHI 164 (clone 13) cells, binds the p55 and p75 TNF receptors on cell lines, induces intercellular adhesion molecule-1 (ICAM-1) expression, and interleukin-6 (IL-6) and granulocyte-macrophage colony-stimulating factor (GM-CSF) release from human umbilical vein endothelial cells (HUVEC). We demonstrate its usefulness for further investigations as an effective reagent for in vitro assays.
Zeman
K
K
Department of Pathophysiology and Immunology, Military Medical Academy, Lódź, Poland.
Paleolog
E M
EM
Tchórzewski
H
H
Szymańska
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002051:Burkitt Lymphoma / Q000188:drug therapy; D002626:Chemistry, Pharmaceutical; D004730:Endothelium, Vascular / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D018124:Receptors, Tumor Necrosis Factor / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D014471:Umbilical Veins / Q000187:drug effects
D000911:Antibodies, Monoclonal; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha