title: Cloning of VH and VL genes for antibodies specific for pyrimidine photo dimers.
abstract: We have prepared two types of cDNAs encoding mouse immunoglobulin heavy (VH) and light (VL) chain variable (V) domains against UV-induced DNA damage by the polymerase chain reaction (PCR). The amplified cDNA was cloned for sequencing and expression, and the nucleotide sequences of the V domains were determined. The deduced amino acid sequences of the complementarity-determining regions (CDRs) indicated that CDR3 of the VH domain and CDR2 of the VL domain might be concerned with the recognition of photolesions.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Morioka
authors.forename: H
authors.initials: H
authors.lastname: Miura
authors.forename: H
authors.initials: H
authors.lastname: Kamiya
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: O
authors.initials: O
authors.lastname: Nikaido
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
mesh_terms: D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011740:Pyrimidine Dimers / Q000276:immunology*; D014466:Ultraviolet Rays
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D011740:Pyrimidine Dimers