title: Binding sites for the herpes simplex virus immediate-early protein ICP4 impose an increased dependence on viral DNA replication on simple model promoters located in the viral genome.
abstract: We examined the ability of binding sites for the herpes simplex virus immediate-early protein ICP4 to alter the regulation of closely linked promoters by placing strong ICP4 binding sites upstream or downstream of simple TATA promoters in the intact viral genome. We found that binding sites strongly reduced the levels of expression at early times postinfection and that this effect was partially overcome after the onset of viral DNA replication. These data confirm that DNA-bound ICP4 can inhibit the activity of a closely linked promoter and raise the possibility that ICP4 binding sites contribute to temporal regulation during infection.
authors.affiliation: Department of Biology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: K E
authors.initials: KE
authors.lastname: Koop
authors.forename: J
authors.initials: J
authors.lastname: Duncan
authors.forename: J R
authors.initials: JR
authors.lastname: Smiley
mesh_terms: D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D001483:Base Sequence; D004261:DNA Replication; D004274:DNA, Recombinant; D016679:Genome, Viral; D005982:Glutathione Transferase / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics*; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D016385:TATA Box / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014709:Vero Cells
chemical_list: D004274:DNA, Recombinant; D017874:Immediate-Early Proteins; D011993:Recombinant Fusion Proteins; C048925:herpes simplex virus, type 1 protein ICP4; D016590:Aphidicolin; D005982:Glutathione Transferase