Reverse cholesterol transport in the rat: influence of short-term D-myo-inositol-1,2,6 trisphosphate (PP56) infusion.
D-myo-inositol-1,2,6 trisphosphate (PP56) was infused in rats for 3 hr at a rate of 30.28 microM/hr/kg body weight. The cholesterol content in plasma, erythrocytes, the aorta, and the liver was monitored before and at the end of the infusion and at 1 and 21 hr after the infusion. We found that the aortic total and free cholesterol content was reduced after the infusion and continuously until the end of the experiment (by 27% each after 21 hr P < 0.05, P < 0.01, respectively). Erythrocyte cholesterol was increased by 6.6% (P < 0.05) at the end of the infusion and 9.5% (P < 0.01) at the end of the experiment, while plasma and high-density lipoprotein cholesterol did not change significantly. Free cholesterol in the liver after infusion was decreased by 27% (P < 0.001) and cholesterol ester increased by 97% (P < 0.05) and did not return to the preinfusion level. Short-term PP56 infusion in the rat apparently influences cholesterol transport in a way which would suggest a promoted reverse cholesterol transport. Since the lipid values did not return to the preinfusion level, a long-term study is needed to elucidate in more detail the influence of PP56 on cholesterol metabolism in vivo.
Wang
W Q
WQ
Department of Internal Medicine, University Hospital, Lund, Sweden.
Wang
X D
XD
Gustafson
A
A
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001692:Biological Transport; D002784:Cholesterol / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D007262:Infusions, Intravenous; D007295:Inositol Phosphates / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D007295:Inositol Phosphates; D002784:Cholesterol; C059368:atrinositol