title: Antigen sensitivity evaluated by tear-specific and serum-specific IgE, skin tests, and conjunctival and nasal provocation tests in patients with ocular allergic disease.
abstract: The potential for ocular allergic patients to have a site-specific antigen sensitisation was investigated using various diagnostic tests of allergen sensitivity in subjects with allergic conjunctivitis (AC: n = 135), vernal keratoconjunctivitis (VK: n = 20), rhinoconjunctivitis (n = 20) or rhinitis (N = 10). In the AC and VK patients, skin tests and conjunctival provocation tests (CPT) were performed, and the levels of specific IgE in serum and in tears were identified. A subgroup of 36 patients was also challenged with a nasal-specific provocation test (NPT). Results showed a poor correlation between skin test results and tear-specific IgE, and also between serum-specific IgE and tear-specific IgE in both AC and VK patients (K < 0.3). CPT and tear IgE were significantly correlated (K = 0.5) in the ocular allergic population. In patients with rhinoconjunctivitis or rhinitis, and in 10 normal subjects, results of CPT and NPT were in 100% agreement. Conversely, in patients with only conjunctivitis, little correlation was found between the results of CPT and NPT (K = 0.3). Tear-specific IgE was the only positive diagnostic sign of antigen sensitivity in 35% of VK patients and 30% of AC patients. These results suggest that the conjunctiva can be a uniquely sensitised target organ in allergic patients.
authors.affiliation: Department of Ophthalmology, University of Padua, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Leonardi
authors.forename: M C
authors.initials: MC
authors.lastname: Battista
authors.forename: M
authors.initials: M
authors.lastname: Gismondi
authors.forename: I A
authors.initials: IA
authors.lastname: Fregona
authors.forename: A G
authors.initials: AG
authors.lastname: Secchi
mesh_terms: D003228:Conjunctiva / Q000276:immunology*; D003233:Conjunctivitis, Allergic / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D009299:Nasal Provocation Tests; D012220:Rhinitis / Q000276:immunology; D012882:Skin Tests; D013666:Tears / Q000276:immunology*
chemical_list: D007073:Immunoglobulin E