Reversed-phase high-performance liquid chromatography determination of quinine in plasma, whole blood, urine, and samples dried on filter paper.
The analysis of quinine in whole blood, plasma, urine, and samples dried on filter paper is described. Extraction was made with toluene followed by back-extraction into phosphate buffer. A reversed-phase liquid chromatography system with fluorescence detection was used. The within-day coefficient of variation of the method was 4-10% at the lower limit of determination (2 nM in plasma and 50 nM in whole blood, dried samples, and urine) and 2-4% at 10 microM. The quinine concentration was found to be lower in whole blood than in plasma (mean ratio, plasma-whole blood, 1.17). The concentration in capillary blood was lower than that in venous blood (mean ratio, capillary blood-venous blood, 0.93).
Ericsson
O
O
Department of Clinical Pharmacology, Huddinge University Hospital, Sweden.
Fridén
M
M
Hellgren
U
U
Gustafsson
L L
LL
D002196:Capillaries / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005374:Filtration; D006801:Humans; D010209:Paper; D011803:Quinine / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014680:Veins / Q000378:metabolism
D011803:Quinine