Characterization of the cell wall of the sheathed methanogen Methanospirillum hungatei GP1 as an S layer.
The cell wall of Methanospirillum hungatei GP1 is a labile structure that has been difficult to isolate and characterize because the cells which it encases are contained within a sheath. Cell-sized fragments, 560 nm wide by several micrometers long, of cell wall were extracted by a novel method involving the gradual drying of the filaments in 2% (wt/vol) sodium dodecyl sulfate and 10% (wt/vol) sucrose in 50 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) buffer containing 10 mM EDTA. The surface was a hexagonal array (a = b = 15.1 nm) possessing a helical superstructure with a ca. 2.5 degrees pitch angle. In shadowed relief, the smooth outer face was punctuated with deep pits, whereas the inner face was relatively featureless. Computer-based two-dimensional reconstructed views of the negatively stained layer demonstrated 4.0- and 2.0-nm-wide electron-dense regions on opposite sides of the layer likely corresponding to the openings of funnel-shaped channels. The face featuring the larger openings best corresponds to the outer face of the layer. The smaller opening was encircled by a stalk-like mass from which 2.2-nm-wide protrusions were resolved. The cell wall in situ was degraded at pH 9.6 at 56 degrees C but was unaffected at pH 7.4 at the same temperature. The cell wall was composed of two nonglycosylated polypeptides (114 and 110 kDa). The cell wall resembled an archaeal S layer and may function in regulating the passage of small (< 10-kDa) sheath precursor proteins.
Firtel
M
M
Department of Microbiology, College of Biological Sciences, University of Guelph, Ontario, Canada.
Southam
G
G
Harauz
G
G
Beveridge
T J
TJ
D001426:Bacterial Proteins / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D017018:Methanomicrobiales / Q000737:chemistry / Q000648:ultrastructure*; D008854:Microscopy, Electron; D017348:Microscopy, Electron, Scanning Transmission; D008954:Models, Biological; D016624:Negative Staining; D013499:Surface Properties
D001426:Bacterial Proteins