Treatment of systemic candidiasis in a neutropenic murine model using immunoglobulin G bearing liposomal amphotericin B.
Efficacy of immunoglobulin G (IgG) bearing liposomal amphotericin B (LAMB-IgG), liposomal amphotericin B without IgG (LAMB) or free amphotericin B (fAMB/Fungizone) was investigated in the treatment of systemic candidiasis in a neutropenic mouse model. Treatment with a single dose (0.6 or 0.9 mg amphotericin B per kg body weight) of LAMB-IgG resulted in a significant increase in the survival rate of neutropenic mice infected with 3 x 10(5) cfu of Candida albicans compared to untreated controls, mice injected with IgG, or liposome alone. Survival was also better in neutropenic mice treated with LAMB-IgG than in neutropenic mice treated with the same dose of LAMB or fAMB. Moreover, 65% of all mice survived the infection after treatment with a single dose of 0.6 mg AMB of the LAMB-IgG formulation. Quantitative culture counts of organs showed that both fAMB and LAMB-IgG formulations even at a dose of 0.3 mg AMB/kg, cleared C. albicans from the spleens, livers, and lungs but not from the kidneys. However, a decreased number of C. albicans cells was recovered from the kidneys of mice that survived the infection. Results of the study suggest that LAMB-IgG is more effective than LAMB or fAMB in the therapy of disseminated candidiasis in neutropenic mice.
Belay
T
T
Department of Botany and Plant Pathology, Michigan State University, East Lansing.
Hospenthal
D R
DR
Rogers
A L
AL
Patterson
M J
MJ
D000666:Amphotericin B / Q000008:administration & dosage*; D000818:Animals; D002177:Candidiasis / Q000188:drug therapy* / Q000209:etiology / Q000628:therapy*; D003131:Combined Modality Therapy; D004353:Drug Evaluation, Preclinical; D005069:Evaluation Studies as Topic; D005260:Female; D007074:Immunoglobulin G / Q000008:administration & dosage*; D008081:Liposomes; D051379:Mice; D009503:Neutropenia / Q000150:complications; D009894:Opportunistic Infections / Q000188:drug therapy* / Q000209:etiology / Q000628:therapy*
D007074:Immunoglobulin G; D008081:Liposomes; D000666:Amphotericin B