Comparison of effects of 0.5 and 3.0 Gy X-irradiation on lipid peroxidation and antioxidant enzyme function in rat testis and liver.
The prooxidant effect of X-irradiation on rat testis and liver tissue was studied with doses of 0.5 and 3.0 Gy; the latter dose kills the proliferating spermatogonia and causes a maturation-depletion process in the germ cells. The level of lipid peroxidation, measured by the formation of diene conjugates and thiobarbituric acid-reactive substances (TBARS) and the activities of the antioxidant enzymes were determined 0.5 hours, 1 day, 7 days, and 31 days after the exposure. In the liver, increased levels of diene conjugation (+36%, P < 0.05) in the group of 3.0 Gy at 0.5 hours indicated increased lipid peroxidation. At the same time, TBARS were increased (+25%, P < 0.05) in the group of 0.5 Gy, but not in the 3.0-Gy group. In the testis, diene conjugation was not determined at 0.5 hours postirradiation, and at day 1 it was at the control level. The level of TBARS in the testis was below control (-11%, P < 0.01) in the 3.0-Gy group at day 1. At day 31 after 3.0 Gy in the testis, an increase in the amount of conjugated dienes (+24%, P < 0.01) was observed in parallel with a decreased level of TBARS (-15%, P < 0.01). The activity of superoxide dismutase (SOD) was decreased in the testis at 0.5 hours postirradiation (-28%, P < 0.05, and -29%, P < 0.05, in the groups of 0.5 and 3.0 Gy), whereafter it returned to normal by day 7. In the liver, such inactivation of SOD was not observed.(ABSTRACT TRUNCATED AT 250 WORDS)
Peltola
V
V
Department of Physiology, University of Turku, Finland.
Parvinen
M
M
Huhtaniemi
I
I
Kulmala
J
J
Ahotupa
M
M
D000818:Animals; D005979:Glutathione Peroxidase / Q000032:analysis / Q000502:physiology*; D005982:Glutathione Transferase / Q000032:analysis / Q000502:physiology*; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D009929:Organ Size / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000032:analysis / Q000502:physiology*; D013737:Testis / Q000201:enzymology* / Q000378:metabolism* / Q000528:radiation effects; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase