title: The OCT plasmid encodes D-lysine membrane transport and catabolic enzymes in Pseudomonas putida.
abstract: Pseudomonas putida (oleovorans) (Pp(OCT)) cured of its OCT plasmid (Pp) no longer grows on D-lysine. Conjugation of PpTrp- with three different methionine auxotrophs carrying the OCT plasmid resulted in PpTrp- (OCT) organisms that grew on D-lysine. Three early D-lysine catabolic enzymes encoded by the OCT plasmid are a lysine racemase, the proposed conversion of D-lysine to delta 1-piperidine-2-carboxylate (P2C), for which we provide evidence, and P2C reductase which converts P2C to pipecolate. In Pp the rate of active D-lysine transport is reduced by 30 to 50%. We consider this to reflect a loss of the gene for the D-lysine carrier while the remaining activity is due to a chromosomally coded L-lysine or D-ornithine carrier or both. The membrane carriers and catabolic enzymes for D- and L-lysine in P. putida P2 and for L-lysine in Pp(OCT) are chromosomally coded.
authors.affiliation: Department of Chemistry, Indiana University of Pennsylvania 15705.
authors.forename: X
authors.initials: X
authors.lastname: Cao
authors.forename: J
authors.initials: J
authors.lastname: Kolonay
authors.forename: K A
authors.initials: KA
authors.lastname: Saxton
authors.forename: R A
authors.initials: RA
authors.lastname: Hartline
mesh_terms: D000591:Amino Acid Isomerases / Q000235:genetics / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D002262:Carboxy-Lyases / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003227:Conjugation, Genetic; D008239:Lysine / Q000378:metabolism*; D016296:Mutagenesis; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism*; D012443:Saccharopine Dehydrogenases / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D000587:Oxidoreductases Acting on CH-NH Group Donors; D012443:Saccharopine Dehydrogenases; C032772:1,2-didehydropipecolate reductase; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D000591:Amino Acid Isomerases; C084211:lysine racemase; D008239:Lysine