title: The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron.
abstract: The gene encoding toxin gamma from the scorpion, Tityus serrulatus, was amplified by PCR from genomic DNA employing synthetic oligonucleotides designed from the reported cDNA sequence. The nucleotide sequence of this gene reveals the presence of an intron of 475 base pairs (bp) which interrupts the region that encodes the signal peptide of the precursor toxin. A comparison of the intron boundary sequences of the gamma toxin gene with ones from other arachnid genes is also presented.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca.
authors.forename: B
authors.initials: B
authors.lastname: Becerril
authors.forename: M
authors.initials: M
authors.lastname: Corona
authors.forename: M C
authors.initials: MC
authors.lastname: Mejía
authors.forename: B M
authors.initials: BM
authors.lastname: Martin
authors.forename: S
authors.initials: S
authors.lastname: Lucas
authors.forename: F
authors.initials: F
authors.lastname: Bolivar
authors.forename: L D
authors.initials: LD
authors.lastname: Possani
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000737:chemistry; D007438:Introns; D016133:Polymerase Chain Reaction; D012604:Scorpion Venoms / Q000737:chemistry / Q000235:genetics*; D012605:Scorpions / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012604:Scorpion Venoms; D004247:DNA