title: Characterization of the adenosine receptors of the rat superior cervical ganglion.
abstract: 1. Adenosine analogues caused hyperpolarization and inhibition of the depolarizing response to muscarine of the rat isolated superior cervical ganglion (SCG) measured by a 'grease gap' recording technique. The receptors mediating these responses have been characterized by use of a range of selective adenosine analogues and adenosine receptor antagonists. 2. In decreasing order of potency N6-cyclopentyladenosine (CPA), 2-chloroadenosine (2CA), adenosine, 2-phenylaminoadenosine (PAA), caused concentration-dependent hyperpolarizations whilst N6-(9-fluorenylmethyl)adenosine (PD 117,413) was inactive at up to 100 microM. 3. The order of potency of adenosine analogues in depressing depolarization caused by a submaximal concentration of muscarine (100 nM) was: CPA > R-PIA = 2CA > NECA > S-PIA > BZA > adenosine > PAA, where R- and S-PIA = R(-)- and S(+)-N6-(2-phenylisopropyl)adenosine, NECA = 5'N-ethylcarboxamidoadenosine and BZA = N6-benzyladenosine. PD 117,413 was inactive at concentrations up to 100 microM. The maximum inhibitions of the muscarine-induced depolarization by CPA, 2CA, NECA and BZA were similar. R-PIA, S-PIA and PAA produced similar maximal inhibitions which were significantly smaller than those produced by CPA. 4. Hyperpolarizations caused by adenosine were antagonized by the P1-purinoceptor selective antagonist 1,3-dimethyl-8-phenylxanthine (8PT) and by the selective A1-adenosine receptor antagonist, 1,3-dipropyl-8-(4-((2-aminoethyl)amino)carbonylmethyloxyphenyl++ +)xanthine (XAC). Hyperpolarizations caused by CPA, adenosine and PAA were antagonized by the A1-selective antagonist, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) but not by the A2-selective antagonist, 3,7-dimethyl-1-propargylxanthine (DMPX). 5. Inhibition of the muscarinic-induced depolarization by CPA was antagonized by 8PT and DPCPXbut not by DMPX.6. It is concluded that the neurones of the rat SCG possess P1-purinoceptors of the Al-adenosine receptor subtype which mediate hyperpolarization and inhibition of depolarization caused by muscarine.
authors.affiliation: Department of Pharmacology, Glasgow University.
authors.forename: G P
authors.initials: GP
authors.lastname: Connolly
authors.forename: T W
authors.initials: TW
authors.lastname: Stone
authors.forename: F
authors.initials: F
authors.lastname: Brown
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009116:Muscarine / Q000494:pharmacology; D010277:Parasympathomimetics / Q000037:antagonists & inhibitors / Q000494:pharmacology; D058915:Purinergic P1 Receptor Antagonists; D051381:Rats; D017208:Rats, Wistar; D018047:Receptors, Purinergic P1 / Q000187:drug effects*; D017783:Superior Cervical Ganglion / Q000187:drug effects*
chemical_list: D010277:Parasympathomimetics; D058915:Purinergic P1 Receptor Antagonists; D018047:Receptors, Purinergic P1; D009116:Muscarine; D000241:Adenosine