Flow injection determination of glucose, bile acid and ATP using immobilized enzyme reactor and chemiluminescent assay of NAD(P)H.
We have developed a chemiluminescent flow injection method for analysis of bile acid, glucose and ATP using the chemiluminescent assay of NADH using 1-methoxy-5-methylphenazinium methyl sulphate (1-MPMS)/isoluminol(IL)/microperoxidase (m-POD) system and immobilized enzyme reactors such as 3 alpha-hydroxysteroid dehydrogenase, glucose-dehydrogenase, hexokinase and glucose-6-phosphate dehydrogenase. The standard curves were obtained in the range of 5-100 pmol for bile acid, 0.5-5.0 nmol for glucose and 10(-7)-10(-5) mol/L for ATP. The coefficient of variation for each assay was not more than 4.1% for bile acid, 2.3% for glucose and 5.3% for ATP, respectively.
Maeda
M
M
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Tsuji
A
A
Ohshima
N
N
Hukuoka
M
M
D015096:3-Hydroxysteroid Dehydrogenases; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); D000255:Adenosine Triphosphate / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis*; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D009243:NAD / Q000032:analysis*; D009249:NADP / Q000032:analysis*; D010084:Oxidation-Reduction
D001647:Bile Acids and Salts; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D015096:3-Hydroxysteroid Dehydrogenases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); D006593:Hexokinase; D005947:Glucose