title: Epstein-Barr virus subtype distribution in angioimmunoblastic lymphadenopathy.
abstract: The tissues of 16 patients bearing a T-cell lymphoma of angioimmunoblastic lymphadenopathy type (AILD-TCL) were investigated for the distribution of Epstein-Barr virus (EBV) subtypes 1 and 2. EBV-association had been proven in these cases by polymerase chain reaction (PCR) for EBV-DNA, in situ hybridization (ISH) for EBV-encoded small nuclear RNAs (EBER) and immunohistology for EBV-encoded latent membrane protein (LMP). PCR and EBER-ISH produced mostly identical results, but some cases were positive with only one of the 2 methods employed. LMP was detected in a few large cells of 8/13 cases. Twelve cases were investigated for the distribution of EBV subtypes. One case contained EBV genome of subtype 2, 3 cases contained subtype 1 and 4 cases contained both subtypes. Four cases could not be typed. These findings suggest that in AILD, as in AIDS-associated lymphomas and lymphomas of the lethal midline granuloma type, subtype 2 of EBV may occur, perhaps in relation to an immunodysfunction developing progressively in these patients.
authors.affiliation: Pathologisches Institut, Universität Bern.
authors.forename: B
authors.initials: B
authors.lastname: Borisch
authors.forename: M
authors.initials: M
authors.lastname: Caioni
authors.forename: N
authors.initials: N
authors.lastname: Hurwitz
authors.forename: C
authors.initials: C
authors.lastname: Dommann-Scherrer
authors.forename: B
authors.initials: B
authors.lastname: Odermatt
authors.forename: E
authors.initials: E
authors.lastname: Waelti
authors.forename: R H
authors.initials: RH
authors.lastname: Laeng
authors.forename: R
authors.initials: R
authors.lastname: Kraft
authors.forename: J
authors.initials: J
authors.lastname: Laissue
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000382:microbiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012342:RNA, Small Nuclear / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014763:Viral Matrix Proteins / Q000032:analysis
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D012342:RNA, Small Nuclear; D012367:RNA, Viral; D014763:Viral Matrix Proteins