title: Infrared CD of deoxy oligonucleotides. Conformational studies of 5'd(GCGC)3', 5'd(CGCG)3', 5'd(CCGG)3', and 5'd(GGCC)3' in low and high salt aqueous solution.
abstract: Infrared (vibrational) circular dichroism (VCD) spectra have been obtained for the self-complementary tetranucleotides, 5'd(CGCG)3', 5'd(GCGC)3', 5'd(CCGG)3', and 5'd(GGCC)3'. In buffered aqueous solution at low salt concentration, these tetramers exhibit spectra associated with right-handed polymers, although the spectra differ significantly for the four species. In high salt solution, a B-->Z transition occurs in 5'd(CGCG)3', while the other tetranucleotides appear only slightly altered. Temperature dependent studies of these oligonucleotides reveal a greater amount of thermal stability for the tetramers in low salt than for the high salt solutions. VCD intensities computed via the exciton formalism are compared with observed results.
authors.affiliation: Department of Chemistry and Biochemistry, City University of New York, Hunter College, New York 10021.
authors.forename: S S
authors.initials: SS
authors.lastname: Birke
authors.forename: M
authors.initials: M
authors.lastname: Moses
authors.forename: B
authors.initials: B
authors.lastname: Kagalovsky
authors.forename: D
authors.initials: D
authors.lastname: Jano
authors.forename: M
authors.initials: M
authors.lastname: Gulotta
authors.forename: M
authors.initials: M
authors.lastname: Diem
mesh_terms: D001483:Base Sequence; D055592:Biophysical Phenomena; D001703:Biophysics; D002942:Circular Dichroism; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009994:Osmolar Concentration; D012492:Salts; D012996:Solutions; D013055:Spectrophotometry, Infrared; D013696:Temperature; D013816:Thermodynamics; D014867:Water
chemical_list: D009838:Oligodeoxyribonucleotides; D012492:Salts; D012996:Solutions; D014867:Water