[The applicability of a new stable fluorescence reagent CGE (N) for TLC measurements and spectrofluorimetry].
A new stable fluorescent reagent, CGE (N), was designed and synthesized in our laboratory. This paper presents the results of using it to detect a compound having aminogroup. Glycine as an example was derived by using CGE (N), the best condition of derivatisation reaction was pH8, T 50 +/- 1 degree C, and time 3-5 h. Spread agent was CHCl3: CH3OH:CH3COOC2H5: iceHAc (1:3:5:1). Rf of glycine-CGE (N) was 0.45, of which fluorescent spectrum Em was 356nm (at Ex = 277nm). The correlation coefficient between the fluorescent scanning area and quantity was over 0.99, the linear relation retained for 24 h, the detection limit 0.24 microgram, and the linear range 0.24-19.2 micrograms. The data revealed that stability, sensitivity and reproducibility were good. These indicate that CGE(N) is a fine fluorescent reagent for compounds having primary amino-group and this method is reliable.
Zou
Z
Z
Wang
H
H
Chen
D
D
Tang
Y
Y
Qiao
X
X
D002855:Chromatography, Thin Layer; D004852:Epoxy Compounds; D005456:Fluorescent Dyes; D009284:Naphthols; D013050:Spectrometry, Fluorescence
D004852:Epoxy Compounds; D005456:Fluorescent Dyes; D009284:Naphthols; C084420:4-chloronaphthyl glycidyl ether