Chronic opioid treatment may uncouple opioid receptors and G-proteins: evidence from radiation inactivation analysis.
Radiation inactivation (target size analysis) was used in this study to determine whether uncoupling of opioid receptor and G-protein is a contributing mechanism to opioid tolerance. Male Sprague-Dawley rats (160-260 g) were rendered tolerant to morphine or [D-Ala2,D-Leu5]enkephalin (DADLE) by multiple i.p. or i.c.v. injections twice a day for 6 or 5 days. Control rats were injected with saline instead of opioids. The animals were killed, the midbrains excised and pooled together for each group. The washed P2 membranes were suspended in buffer and irradiated with 1-10 Mrad doses of 60Co irradiation, following which mu- or delta-opioid receptor binding activity of each sample was assayed. The molecular weight of the receptor was calculated from a standard irradiation curve constructed using several enzyme markers of known molecular weight. We found that the functional molecular size of mu-opioid receptor significantly decreased from 349 kDa to 228 kDa after 6 days of chronic morphine treatment, while, the molecular size of delta-opioid receptor decreased from 303 kDa to 223 kDa after 5 days of chronic DADLE treatment. These results are consistent with the uncoupling of opioid receptor from G-protein during chronic opioid treatment.
Tao
P L
PL
Department of Pharmacology, National Defense Medical Center, Taipei, Taiwan, ROC.
Chang
L R
LR
Chou
Y P
YP
Law
P Y
PY
Loh
H H
HH
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004361:Drug Tolerance; D016308:Enkephalin, Leucine-2-Alanine / Q000008:administration & dosage*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D008297:Male; D009020:Morphine / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D013997:Time Factors
D011957:Receptors, Opioid; D006165:Guanylyl Imidodiphosphate; D016308:Enkephalin, Leucine-2-Alanine; D009020:Morphine; D012964:Sodium; D019204:GTP-Binding Proteins