title: Patch length of localized repair events: role of DNA polymerase I in mutY-dependent mismatch repair.
abstract: In vivo experiments with heteroduplex lambda genomes show that the MutY mismatch repair system of Escherichia coli defines an average repair tract that is shorter than 27 nucleotides and longer than 9 nucleotides and extends 3' from the corrected adenine. The phenotype of a mutant defective in DNA polymerase I shows that this enzyme plays a significant, though not an essential, role in the in vivo repair of apurinic sites generated by this system. Evidence is presented that in the absence of polymerase I the repair tracts are modestly longer than in the polA+ extending in the 5' direction from the corrected adenine, suggesting a role for another DNA polymerase.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: J P
authors.initials: JP
authors.lastname: Radicella
authors.forename: E A
authors.initials: EA
authors.lastname: Clark
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: M S
authors.initials: MS
authors.lastname: Fox
mesh_terms: D000251:Adenosine Triphosphatases; D001426:Bacterial Proteins / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D015169:Colony Count, Microbial; D045647:DNA Glycosylases; D004256:DNA Polymerase I / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D000070956:MutL Proteins; D016254:Mutagenesis, Insertional; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D009692:Nucleic Acid Heteroduplexes
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C074770:MutL protein, E coli; D009692:Nucleic Acid Heteroduplexes; D004256:DNA Polymerase I; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C061738:mutY adenine glycosylase; D000251:Adenosine Triphosphatases; D000070956:MutL Proteins