Catabolite repression of beta-glucanase synthesis in Bacillus subtilis.
beta-Glucanase synthesis in Bacillus subtilis was repressed by glucose and other substrates of glycolysis. Experiments with different pts mutants showed that the phosphoenolpyruvate: sugar phosphotransferase system is not involved in carbon catabolite repression of beta-glucanase synthesis. Carbon catabolite repression of beta-glucanase synthesis was completely abolished in a ccpA mutant. An operator structure similar to those upstream of amyE and the xyl operon was found and was shown by site-directed mutagenesis to be the target for carbon catabolite repression of beta-glucanase synthesis. The presence of this operator on a multi-copy plasmid resulted in a reduced repression of both beta-glucanase and alpha-amylase synthesis. It seems likely that the gene encoding these enzymes are part of one regulon with respect to catabolite repression.
Krüger
S
S
Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, Germany.
Stülke
J
J
Hecker
M
M
D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002241:Carbohydrates / Q000494:pharmacology; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005947:Glucose / Q000494:pharmacology; D006019:Glycolysis; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D018085:Regulon
D002241:Carbohydrates; D004269:DNA, Bacterial; D006026:Glycoside Hydrolases; C028020:licheninase; D005947:Glucose