Transcriptional regulation of the c-erbB-3 gene in human breast carcinoma cell lines.
The c-erbB-3 gene is a member of the EGF receptor family of membrane tyrosine kinases. Previous work has shown that the expression of this gene is frequently unregulated in human breast carcinoma-derived cell lines and in tumour biopsy material. As there is no evidence of gene amplification, we decided to examine the control of c-erbB-3 transcription in overexpressing cells. In this paper we describe the cloning of the c-erbB-3 promoter and its functional analysis within mammary-derived cell lines. We show that the promoter is more active in overexpressing cells and this is largely due to the action of the OB2-1 transcription factor which we have recently identified as mediating overexpression of the c-erbB-2 promoter in similar cell lines.
Skinner
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Gene Transcription Laboratory, Hammersmith Hospital, London.
Hurst
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HC
D000595:Amino Acid Sequence; D001483:Base Sequence; D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000494:pharmacology; D066246:ErbB Receptors / Q000032:analysis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D020893:Receptor, ErbB-3; D014157:Transcription Factors / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C081684:OB2-1 protein, human; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D066246:ErbB Receptors; D020893:Receptor, ErbB-3