title: [Superoxide dismutase in the nuclei of cerebral cortex cells of rats: isolation, properties, effect of ionizing radiation].
abstract: Superoxide dismutase (SOD) from rat brain cell nuclei has been distinguished. After chromatography on hydroxylapatite Sephacryl S200 and Mono S SOD can be purified 1200 times. It is Mn-depending enzyme, which has molecular weight 10 kDa and firmly associated with transcriptionally active chromatin. SOD is most active at high ion strength (1.2-2 M NaCl). The more sensitive method for SOD activity determination is suggested (modification of adrenaline autoxidation at pH 10.2).
authors.forename: A F
authors.initials: AF
authors.lastname: Protas
authors.forename: P P
authors.initials: PP
authors.lastname: Chaialo
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology* / Q000648:ultrastructure; D005720:Gamma Rays; D008297:Male; D008970:Molecular Weight; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification* / Q000528:radiation effects
chemical_list: D013482:Superoxide Dismutase
vernacular_title: Superoksiddismutaza iader kletok kory golovnogo mozga krys: vydelenie, svoĭstva, vliianie ioniziruiushcheĭ radiatsii.