title: Nucleotide sequence analysis of four genes, hupC, hupD, hupF and hupG, downstream of the hydrogenase structural genes in Bradyrhizobium japonicum.
abstract: The nucleotide sequence of a 2.2 kb region downstream of the hydrogenase structural genes in Bradyrhizobium japonicum was determined. Four genes encoding predicted polypeptides of 27.8 (HupC), 21.4 (HupD), 10.6 (HupF) and 15.8 (HupG) kDa were identified, of which the first three probably belong to the same operon as the hup structural genes, hupS and hupL. HupC is homologous to the hydrophobic polypeptides with four potential transmembrane regions that are encoded by open reading frames following the hydrogenase structural genes in Rhodobacter capsulatus, Escherichia coli, Azotobacter vinelandii, Wolinella succinogenes, Rhizobium leguminosarum and Alcaligenes eutrophus. Also HupD, HupF and HupG are homologous to genes involved in processing, maturation, functioning and regulation of hydrogenase activity in various hydrogen-oxidizing bacteria.
authors.affiliation: F. A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Van Soom
authors.forename: J
authors.initials: J
authors.lastname: Browaeys
authors.forename: C
authors.initials: C
authors.lastname: Verreth
authors.forename: J
authors.initials: J
authors.lastname: Vanderleyden
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012230:Rhizobiaceae / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D006864:Hydrogenase