Phosphorylated nutrient uptake by Treponema denticola and other potential periodontopathogens.
Nutrient uptake may contribute to the survival of certain potential periodontopathogens in the competitive environment of the gingival sulcus. This study was conducted to assess the uptake of commercially available, key radiolabelled phosphorylated compounds by Treponema denticola. There was a linear relationship in the uptake of ATP and G-1-P with cell concentrations of 0.1-2.5 mg of cell dry weight. This uptake reached a steady state within 15 min, and at temperatures of 25-37 degrees C the uptake ranged between 13-21%. At 50 degrees C, or above 50 degrees C the assay cells took up 0-1% of the assay nutrients. The maximum uptake of ATP was 47.5, 38 and 47.6 pmoles for serovars a, b and c. G-1-P yielded maximum uptake values of 306, 304, and 306 pmoles respectively. Significant uptake of ATP, or G-1-P occurred at pH 4.8 for serovars a, or c, while serovar b showed a broad pH range for the uptake of G-1-P. Stannous fluoride at a concentration of 0.17 mM enhanced the uptake of ATP while chlorhexidine digluconate inhibited the uptake of ATP. At concentrations usually employed (0.05-0.5 mM) the metabolic poisons 2,4-dinitrophenol, sodium azide, sodium arsenite and the ATPase inhibitor N,N1-dicyclohexylcarbodiimide did not alter significantly the uptake of ATP. The uptake of ATP, by Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum and T. denticola at pH 4.8 was 13.4, 1.5, 6.9, and 18-20.4%, respectively. At pH 6.5 the uptake for the microorganisms indicated above was 22, 1.3, 6.6 and 6.2%, respectively. G-1-P showed the same trend in its uptake by A. actinomycetemcomitans, P. gingivalis, F. nucleatum and T. denticola. This study provides for the first time specific examples of phosphorylated nutrient uptake by potential periodontopathogens, and that the uptake of phosphorylated nutrients can be suppressed by oral hygienic compounds.
Yotis
W W
WW
Department of Microbiology, Loyola University Medical Center, Maywood, Illinois 60153.
Gopalsami
C
C
Corrigan
K
K
Hoerman
K
K
Keene
J J
JJ
D000255:Adenosine Triphosphate / Q000493:pharmacokinetics*; D016976:Aggregatibacter actinomycetemcomitans / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D016967:Fusobacterium nucleatum / Q000378:metabolism; D005958:Glucosephosphates / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D010766:Phosphorylation; D016966:Porphyromonas gingivalis / Q000378:metabolism; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors; D014002:Tin Fluorides / Q000494:pharmacology; D014208:Treponema / Q000187:drug effects / Q000378:metabolism*
D002250:Carbon Radioisotopes; D005958:Glucosephosphates; D014002:Tin Fluorides; D000255:Adenosine Triphosphate; C031590:glucose-1-phosphate; C010882:chlorhexidine gluconate; D002710:Chlorhexidine