Direct determination of adamantanamine in plasma and urine with automated solid phase derivatization.
A simple, highly sensitive and selective method is described for adamantanamine determination in plasma and urine by high-performance liquid chromatography with fluorescence detection. The method involved a simultaneous extraction and derivatization of biological fluids with a 9-fluoreneacetate (9-FA) solid-phase derivatization reagent. This approach eliminated tedious sample preparation steps and provided automatic derivatization with selective and efficient sample clean-up for direct injection of biological fluids. Derivatized adamantanamine was separated under conventional reversed-phase conditions and determined by fluorescence detection. The optimization and validation of the derivatization method with the 9-FA solid-phase reagent is described.
Zhou
F X
FX
Department of Chemistry, Northeastern University, Boston, MA 02115.
Krull
I S
IS
Feibush
B
B
D000085:Acetates; D000547:Amantadine / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence
D000085:Acetates; D005449:Fluorenes; D007202:Indicators and Reagents; C078694:9-fluoreneacetic acid; D000547:Amantadine