Non-proteoglycan forms of biglycan increase with age in human articular cartilage.
Polyclonal anti-peptide antibodies were raised to the C-terminal regions of human biglycan and decorin. These antibodies were used in immunoblotting to study structural variations with age in the proteoglycan core proteins present in extracts of human articular cartilage and intervertebral disc. Three forms of the biglycan core protein were identified. The largest form was detected only after chondroitinase treatment and represents the proteoglycan form of the molecule from which the glycosaminoglycan chains have been removed. However, chondroitinase treatment did not alter the electrophoretic mobility of the two smaller proteins, which appear to represent non-proteoglycan forms of the molecule, resulting either from a failure to substitute the intact proteoglycan core protein with glycosaminoglycan chains during its synthesis or from proteolytic processing of the intact proteoglycan causing removal of the N-terminal region bearing the glycosaminoglycan chains. The non-proteoglycan forms constituted a minor proportion of biglycan in the newborn, but were the major components in the adult. A similar trend was seen in both articular cartilage and intervertebral disc. In comparison, decorin appears to exist predominantly as a proteoglycan at all ages, with two core protein sizes being present after chondroitinase treatment. Non-proteoglycan forms were detected in the adult, but they were always a minor constituent.
Roughley
P J
PJ
Genetics Unit, Shriners Hospital for Crippled Children, McGill University, Montreal, Canada.
White
R J
RJ
Magny
M C
MC
Liu
J
J
Pearce
R H
RH
Mort
J S
JS
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D058578:Biglycan; D002358:Cartilage, Articular / Q000196:embryology / Q000378:metabolism*; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005333:Fetus; D006801:Humans; D007223:Infant; D008875:Middle Aged; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011509:Proteoglycans / Q000276:immunology / Q000378:metabolism*
C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D010455:Peptides; D011509:Proteoglycans