title: Bone morphogenetic protein-induced muscle- and synovium-derived cartilage differentiation in vitro.
abstract: Muscle- and synovium-derived mesenchymal-type cells differentiated into cartilage in response to a coating of partially purified rabbit bone morphogenetic protein (BMP) on a substratum of plastic. Observed by means of phase contrast microscopy, light microscope histochemistry, and electron microscopy, the sequence of events on a BMP-coated plastic substratum is the same as that observed on a substratum of bone matrix. The plastic eliminates the possibility of any endogenous allogeneic matrix-derived BMP contaminating the system. The differentiation of cartilage from synoviocytes and subsynovial cells resembles the pathologic process occurring in human chondromatosis.
authors.affiliation: Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: S
authors.initials: S
authors.lastname: Ono
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D001846:Bone Development; D019485:Bone Morphogenetic Proteins; D002356:Cartilage / Q000502:physiology* / Q000648:ultrastructure; D002454:Cell Differentiation; D002478:Cells, Cultured; D006133:Growth Substances / Q000494:pharmacology*; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009132:Muscles / Q000166:cytology* / Q000187:drug effects; D011506:Proteins / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects
chemical_list: D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins