Inhibitory effects of hyaluronan on neutrophil-mediated cartilage degradation.
The effects of hyaluronan on neutrophil-mediated cartilage degradation were studied in an in vitro model. Rat peritoneal neutrophils were incubated for up to 18 h with the neutralized cartilage of bovine nasal septa using N-formyl-L-methionyl-L-leucyl-L-phenylalanine combined with cytochalasin B, or opsonized zymosan, as a stimulation agent of neutrophils. Hyaluronan inhibited the neutrophil-mediated cartilage degradation by reducing the release of sulfated glycosaminoglycans from the cartilage. Inhibitory effects were dependent on concentration and molecular weight of hyaluronan. These results suggest that hyaluronan with a high molecular weight plays an important role in protecting the articular cartilage in inflamed joints from neutrophil injury.
Tobetto
K
K
Research Laboratories, Maruho Co., Ltd., Osaka, Japan.
Nakai
K
K
Akatsuka
M
M
Yasui
T
T
Ando
T
T
Hirano
S
S
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002417:Cattle; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006025:Glycosaminoglycans / Q000378:metabolism; D006820:Hyaluronic Acid / Q000032:analysis / Q000494:pharmacology* / Q000502:physiology; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013582:Synovial Fluid / Q000737:chemistry / Q000502:physiology; D015054:Zymosan / Q000494:pharmacology
D006025:Glycosaminoglycans; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006820:Hyaluronic Acid; D015054:Zymosan