title: Developmental expression of the alpha 6 GABAA receptor subunit mRNA occurs only after cerebellar granule cell migration.
abstract: Using a competitive polymerase chain reaction (PCR) and appropriate internal standards, we have analyzed absolute amounts of the alpha 6 GABAA receptor subunit mRNA in the postnatally developing cerebellum and neocortex. The PCR data have shown that absolute amounts of the alpha 6 receptor subunit mRNA in the cerebellum increase dramatically (nearly 100-fold) during the second postnatal week, reaching maximal levels by postnatal day 21 (1 fmol/microgram total RNA). The absolute amount of the alpha 6 GABAA receptor subunit mRNA in the cortex at postnatal day 1 was 2 amol/microgram total RNA and increased to 7 amol/micrograms total RNA by postnatal day 14. No further increase in alpha 6 mRNA expression in the adult cortex was observed. Microscopic analysis of emulsion coated and counterstained sections indicated that alpha 6 GABAA receptor subunit mRNA labeling was only detected in the internal granule cell layer and not in either the external granule cell layer or in migrating granule cells. The alpha 1 GABAA receptor subunit mRNA increased in the cerebellar cortex with a similar temporal profile, although its distribution extended to additional cell types (Purkinje cells, stellate/basket cells and possibly cerebellar astrocytes). The temporal expression of these two GABAA receptor subunit mRNAs is coincident with the formation of synaptic contacts in the granule cell dendrites suggesting that afferent pathways innervating these neurons following cell migration may play a critical role in increasing the expression of mRNAs encoding the alpha 1 and alpha 6 GABAA receptor subunits.
authors.affiliation: Fidia-Georgetown Institute for the Neurosciences, Georgetown University, Washington, DC 20007.
authors.forename: T
authors.initials: T
authors.lastname: Zheng
authors.forename: M R
authors.initials: MR
authors.lastname: Santi
authors.forename: P
authors.initials: P
authors.lastname: Bovolin
authors.forename: L N
authors.initials: LN
authors.lastname: Marlier
authors.forename: D R
authors.initials: DR
authors.lastname: Grayson
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D046911:Macromolecular Substances; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000096:biosynthesis*
chemical_list: D046911:Macromolecular Substances; D015347:RNA Probes; D012333:RNA, Messenger; D011963:Receptors, GABA-A