Transcription of ColE1Ap mbeC induced by conjugative plasmids from twelve different incompatibility groups.
Although nonconjugative mobilizable plasmids require helping functions of conjugative plasmids in order to be mobilized into recipients, at least some genes from the nonconjugative plasmids may be induced to assist in the DNA transfer process. Conjugative plasmids from 12 different incompatibility groups mobilized the nonconjugative plasmid ColE1Ap between Escherichia coli strains. Introduction of any of the conjugative plasmids into the ColE1Ap-containing strain resulted in an induction of mbeC, the product of which is a component of the mobilization relaxation complex. Each of the conjugative plasmids caused protein to bind specifically to mbe promoter DNA, suggesting a direct regulatory interaction.
Selvaratnam
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Department of Bioscience and Biotechnology, Drexel University, Philadelphia, Pennsylvania 19104.
Gealt
M A
MA
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D003087:Colicins / Q000037:antagonists & inhibitors; D003227:Conjugation, Genetic; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003087:Colicins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; C007104:colicin immunity proteins