Isolation of the porcine complement activation fragments C3c and C3d and their use in the preparation of monospecific antibodies.
The hemolytic test to date has been the sole analytic technique applied to study the complement reaction in the swine. To improve the analytical possibilities for this species we have developed polyclonal antibody reagents with specificities for the C3c and C3d activation fragments of swine C3. Access to these reagents, by which activation products can be analysed in tissues and biological fluids, will offer new possibilities for a more precise analysis of the complement reaction.
Storm
K E
KE
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden.
Nilsson
U R
UR
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002850:Chromatography, Gel; D003167:Complement Activation; D015932:Complement C3c / Q000276:immunology / Q000302:isolation & purification*; D015933:Complement C3d / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007124:Immunoenzyme Techniques; D011817:Rabbits; D013552:Swine / Q000276:immunology*
D000906:Antibodies; D015932:Complement C3c; D015933:Complement C3d