Kinesin-mediated vesicular transport in a biochemically defined assay.
Here we have described simple and reproducible methods to observe kinesin-mediated vesicle and microtubule movements under defined conditions using video microscopy. We are optimistic that this assay will provide a useful tool to study kinesin function, regulation, and dynamic physical interactions with membranous organelles and microtubules.
Urrutia
R
R
Laboratory of Cellular Biology, National Institute for Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892.
Murphy
D B
DB
Kachar
B
B
McNiven
M A
MA
D000818:Animals; D002837:Chromaffin Granules / Q000502:physiology; D016547:Kinesins / Q000502:physiology*; D008870:Microtubules / Q000502:physiology; D009068:Movement; D015388:Organelles / Q000502:physiology*
D016547:Kinesins