Transcription in vitro promoted by the Agrobacterium VirG protein.
Expression of the virulence genes (vir) on the hairy-root-inducing plasmid pRiA4 is induced by plant signals in Agrobacterium cells through a two-component regulatory system, the VirA-VirG system. We constructed an in vitro transcription system that consisted of the purified VirG protein and the Agrobacterium RNA polymerase holoenzyme. Both versions of VirG, the non-phosphorylated form and the VirA-phosphorylated form, were active but showed different patterns of the pH-dependency for transcriptional activation.
Endoh
H
H
Laboratory of Molecular Genetics, Institute for Chemical Research, Kyoto University, Japan.
Aoyama
T
T
Oka
A
A
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C058483:virG protein, Shigella flexneri; D012321:DNA-Directed RNA Polymerases