Processing of pestivirus polyprotein: cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus.
The polyprotein of classical swine fever virus starts with the nonstructural protein p23, which is followed by the nucleocapsid protein p14. Proteolytic cleavage between p23 and p14 was demonstrated in a cell-free transcription-translation system. Successive truncation of the cDNA used for the transcription indicated that the proteolytic activity responsible for the cleavage between p23 and p14 resides within p23. In order to determine the cleavage site between these two proteins, the respective genomic regions were expressed in two different expression systems. N-terminal sequencing of the resulting p14-related proteins revealed that cleavage occurs between Cys-168 and Ser-169. Comparison of the sequence around the cleavage site with sequences of other pestiviruses suggests a conserved processing site between similar proteins.
Stark
R
R
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Meyers
G
G
Rümenapf
T
T
Thiel
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000302:isolation & purification; D036022:Capsid Proteins; D002474:Cell-Free System; D006692:Classical Swine Fever Virus / Q000378:metabolism*; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D010576:Pestivirus / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis*
D036022:Capsid Proteins; D018076:DNA, Complementary; D011498:Protein Precursors; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C084303:p23 protein, classical swine fever virus; C084302:protein p14, classical swine fever virus