Genes expressed during sexual differentiation of Chlamydomonas reinhardtii.
Four genes specifically expressed during gametogenesis of Chlamydomonas reinhardtii have been cloned and their expression patterns analyzed. mRNAs encoded by these gamete-specific genes (gas) were absent or present only at very low levels in vegetative cells and mature zygotes. In young zygotes 2 h after gamete fusion, the mRNAs of three gas genes still persisted. The gas mRNAs accumulated during gametic differentiation. The temporal patterns of accumulation of individual mRNAs differed; some started to increase early during gametogenesis, others accumulated in the late phase. The accumulation of one of the late mRNAs (gas28) was strictly light-dependent. To illustrate the utility of the genes cloned in the analysis of sexual differentiation in Chlamydomonas reinhardtii we show that in a gametogenesis-defective mutant, the expression of late genes is prevented while that of early genes is normal.
von Gromoff
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ED
Institut für Biologie III, Albert-Ludwigs-Universität, Freiburg, Germany.
Beck
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CF
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D005718:Gametogenesis / Q000235:genetics; D015870:Gene Expression; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger