[Antioxidative action of soyasaponin monomers I, A1, A2, and their inhibitory effects on action potential and beating of myocardiocytes in culture].
Ventricular myocardiocytes from newborn Wistar rats were cultured. Soyasaponin monomers I, A1, A2 2 micrograms.ml-1 added to the culture medium, like nimodipine 1.25 microgram.ml-1, decreased the number of spontaneously beating clusters and the action potential parameters of myocardiocytes, which restored after washing out and were reversed by Ca2+ 80 micrograms.ml-1 or epinephrine 10 micrograms.ml-1, indicating the Ca2+ channel blockade action of the saponin monomers. Adding xanthine 0.42 mmol.L-1 and xanthine oxidase 5.4 nmol.L-1 into culture medium led to the increase of free radical content of the myocardiocytes and the decrease of action potential parameters, which were reversed by I 2 micrograms.ml-1, indicating the antioxidative action of saponin monomer I.
Jiang
Y
Y
Central Laboratory of Physiology, Normal Bethune University of Medical Sciences, Changchun, China.
Zhong
G G
GG
Qi
H
H
Ma
X Y
XY
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D005609:Free Radicals; D009206:Myocardium / Q000166:cytology*; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors; D014970:Xanthines / Q000037:antagonists & inhibitors
D000975:Antioxidants; D002121:Calcium Channel Blockers; D005609:Free Radicals; D012503:Saponins; D014970:Xanthines; C056684:soyasaponin I; D009828:Oleanolic Acid; D014969:Xanthine Oxidase