Alpha (1,3/1,4)fucosyltransferase (FucT-III) gene is inactivated by a single amino acid substitution in Lewis histo-blood type negative individuals.
Human alpha (1,3/1,4)fucosyltransferase (FucT-III) genes were cloned from genomic DNAs of seven Lewis negative (Le(a- b-)) individuals. Restriction mapping analyses revealed that an additional Pvu II site was created in the FucT-III genes from Lewis negative individuals. Four of these individuals were shown to be heterozygous for the Pvu II site and three were homozygous for it. The Pvu II site was produced by a single base substitution at the 508 nucleotide of coding sequence, G to A, resulting in the amino acid substitution, Gly to Ser at position 170. Another single base substitution, resulting in the change of Asp to Ala at position 336, was observed in the Lewis negative FucT-III gene which did not have the Pvu II site. The FucT-III enzyme was inactivated by these two mutations resulting in a single amino acid substitution in the catalytic region. All FucT-III genes, regardless of the Pvu II site, from Lewis negative individuals had a single base substitution, the T at position 59 to G, resulting in the change of Leu to Arg at position 20. This mutation, however, was not responsible for FucT-III gene inactivation.
Nishihara
S
S
Division of Cell Biology, Soka University, Tokyo, Japan.
Yazawa
S
S
Iwasaki
H
H
Nakazato
M
M
Kudo
T
T
Ando
T
T
Narimatsu
H
H
D000595:Amino Acid Sequence; D001120:Arginine; D001483:Base Sequence; D001665:Binding Sites; D001788:Blood Grouping and Crossmatching; D015139:Blotting, Southern; D002240:Carbohydrate Sequence; D002678:Chimera; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D004247:DNA / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D005260:Female; D005647:Fucosyltransferases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D007700:Kinetics; D007930:Leucine; D007962:Leukocytes / Q000201:enzymology; D007983:Lewis Blood Group Antigens / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D014162:Transfection
D017931:DNA Primers; D007983:Lewis Blood Group Antigens; D004247:DNA; D001120:Arginine; D005647:Fucosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase; D007930:Leucine