title: Advanced glycosylation endproduct-specific receptors on human and rat T-lymphocytes mediate synthesis of interferon gamma: role in tissue remodeling.
abstract: During normal aging and in chronic diabetes the excessive accumulation of reactive glucose-protein or glucose-lipid adducts known as advanced glycosylation endproducts (AGEs) has been shown to induce tissue dysfunction, in part through interaction with AGE-specific receptors on monocyte/macrophages and other cells. Recognizing that circulating lymphocytes trafficking through tissues interact with tissue AGEs, we searched for the expression of AGE-binding sites on peripheral blood T lymphocytes. Resting rat and human T cells bound 125I-AGE-albumin with an affinity of 7.8 x 10(7) M-1, whereas, after stimulation with phytohemagglutinin (PHA) for 48 h, binding affinity increased to 5.8 x 10(8) M-1. Flow cytometric analysis of resting rat T cells using polyclonal antibodies raised against rat liver AGE-binding proteins (p60 and p90) revealed the constitutive expression of both immunoreactivities. The number of resting CD4+ and CD8+ T cells positive for anti-p60 antibody binding (34.2 and 58.5%, respectively) increased to 92 and 90% of cells after 48-h stimulation with PHA. Exposure of PHA-activated T lymphocytes to AGE-albumin enhanced expression of interferon gamma (IFN-gamma) mRNA 10-fold and induced greater elaboration of the mature protein than did exposure to unmodified protein or PHA treatment alone. These data indicate that T cells contain an inducible system of surface receptors for AGE-modified proteins, and that receptor occupancy is linked to lymphokine production. This T cell AGE-receptor system might serve to target lymphocytes to AGE-rich tissues and involve them in the regulation of tissue homeostasis either by assisting in macrophage-dependent clearance of AGE-proteins, or by exerting direct antiproliferative action on mesenchymal cells. Under conditions of excessive AGE-protein and AGE lipid accumulation (e.g., aging and diabetes), enhanced production of AGE-induced IFN-gamma may accelerate immune responses that contribute to tissue injury.
authors.affiliation: Picower Institute for Medical Research, Manhasset, New York 11030.
authors.forename: F
authors.initials: F
authors.lastname: Imani
authors.forename: Y
authors.initials: Y
authors.lastname: Horii
authors.forename: M
authors.initials: M
authors.lastname: Suthanthiran
authors.forename: E Y
authors.initials: EY
authors.lastname: Skolnik
authors.forename: Z
authors.initials: Z
authors.lastname: Makita
authors.forename: V
authors.initials: V
authors.lastname: Sharma
authors.forename: P
authors.initials: P
authors.lastname: Sehajpal
authors.forename: H
authors.initials: H
authors.lastname: Vlassara
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005434:Flow Cytometry; D015870:Gene Expression; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D008024:Ligands; D008213:Lymphocyte Activation; D008264:Macrophages / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D009000:Monocytes / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; D017127:Glycation End Products, Advanced; D008024:Ligands; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D007371:Interferon-gamma