title: The influence of indomethacin on the hatching of mouse blastocysts.
abstract: In order to investigate the effect of indomethacin for inhibition of the cyclooxygenase pathway on the hatching process of mouse blastocysts, 508 mouse blastocysts were cultured in modified HAM F10 medium containing 0, 8, 79 or 788 microM indomethacin, added after 24 h of incubation. Hatching was scored after 72 h of incubation. In another series of experiments, indomethacin was added only after blastulation had occurred. Indomethacin in doses of 8 microM and 79 microM, added after 24 h of incubation or after blastulation had occurred, did not influence successful hatching. A dose of 788 microM indomethacin interfered with hatching, but this dose appeared to be toxic for the embryo.
authors.affiliation: Department of Obstetrics, Gynecology and Reproduction, Leiden University Hospital, The Netherlands.
authors.forename: R M
authors.initials: RM
authors.lastname: van der Weiden
authors.forename: R M
authors.initials: RM
authors.lastname: Verdijk
authors.forename: R E
authors.initials: RE
authors.lastname: Poelmann
authors.forename: F M
authors.initials: FM
authors.lastname: Helmerhorst
authors.forename: M J
authors.initials: MJ
authors.lastname: Keirse
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D046508:Culture Techniques; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D010064:Embryo Implantation / Q000187:drug effects; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D051379:Mice
chemical_list: D016861:Cyclooxygenase Inhibitors; D007213:Indomethacin