Localization of a single-stranded RNA-binding domain in the movement protein of red clover necrotic mosaic dianthovirus.
Mutant movement proteins of red clover necrotic mosaic dianthovirus (RCNMV), consisting of in-frame deletions or fusions with a maltose-binding protein, were produced in Escherichia coli using expression vectors. The ability of the mutant proteins to bind to ssRNA was tested by photochemical cross-linking and gel retardation. The results showed that the region between amino acids 181 and 225 of the RCNMV movement protein contains an ssRNA-binding domain.
Osman
T A
TA
Department of Biology, Imperial College of Science, Technology and Medicine, London, U.K.
Thömmes
P
P
Buck
K W
KW
D000595:Amino Acid Sequence; D001483:Base Sequence; D017353:Gene Deletion; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics / Q000502:physiology*; D016296:Mutagenesis; D053830:Plant Viral Movement Proteins; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D053830:Plant Viral Movement Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins