The influence of thalamic GABA transmission on the susceptibility of adult rats to flurothyl induced seizures.
There is considerable evidence that thalamic nuclei are involved in the propagation and regulation of seizures. In the present study, we investigated the possible role in seizure mechanisms of GABAergic transmission in two thalamic nuclei, the posterior nucleus (PO) and the ventromedial nucleus (VM). Several GABAergic drugs were bilaterally microinfused into PO or VM of adult rats via chronically implanted cannulae, before testing the rats' susceptibility to seizures induced by flurothyl. In PO, infusions of the GABA elevating agent gamma-vinyl-GABA (20 micrograms) or of the GABAA receptor agonist muscimol (100 ng) suppressed both clonic and tonic seizures. Infusions into PO of the GABAA receptor antagonist bicuculline (100 ng) facilitated both these seizure types. Administration of the GABAB receptor agonist baclofen (200 ng) also suppressed clonic seizure susceptibility. Drug infusions into VM, however, did not significantly modify the susceptibility to seizures. These findings lead us to conclude that GABAergic transmission in the vicinity of the PO, but perhaps not in VM, affects flurothyl seizure susceptibility. We hypothesize that GABA synapses in PO may be part of a seizure propagation or control circuit including striatum, substantia nigra, and superior colliculus.
Garant
D S
DS
Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.
Xu
S G
SG
Sperber
E F
EF
Moshé
S L
SL
D000614:Aminocaproates / Q000494:pharmacology; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001418:Baclofen / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D001640:Bicuculline / Q000494:pharmacology; D005481:Flurothyl; D008297:Male; D008845:Microinjections; D009118:Muscimol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology* / Q000523:psychology; D009435:Synaptic Transmission / Q000502:physiology*; D013787:Thalamic Nuclei / Q000473:pathology / Q000502:physiology*; D020888:Vigabatrin; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D000614:Aminocaproates; D000927:Anticonvulsants; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D005481:Flurothyl; D020888:Vigabatrin; D001418:Baclofen; D001640:Bicuculline