Histamine potentiates N-methyl-D-aspartate responses in acutely isolated hippocampal neurons.
N-methyl-D-aspartate (NMDA)-evoked currents were recorded from acutely isolated rat hippocampal neurons, using the whole-cell patch-clamp technique and a rapid perfusion system. Histamine, at concentrations from 0.5 to 100 microM, reversibly enhanced NMDA currents by up to 50%. The effect cannot be ascribed to activation of the known histamine receptors (H1, H2, H3) but is occluded by spermine. These results suggest an interaction of histamine with the polyamine-binding site on the NMDA receptor complex. This modulatory action could allow the histaminergic system to determine time and loci of NMDA receptor-mediated events, such as memory formation according to behavioral state.
Vorobjev
V S
VS
Institute of Physiology II, Heinrich-Heine-University, Düsseldorf, Federal Republic of Germany.
Sharonova
I N
IN
Walsh
I B
IB
Haas
H L
HL
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology / Q000502:physiology; D002469:Cell Separation; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004594:Electrophysiology; D005998:Glycine / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology*; D016202:N-Methylaspartate / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009994:Osmolar Concentration; D011968:Receptors, Histamine / Q000502:physiology; D013997:Time Factors
D011968:Receptors, Histamine; D001224:Aspartic Acid; D016202:N-Methylaspartate; D006632:Histamine; D002118:Calcium; D005998:Glycine