title: Release of prostaglandin E2 by human mononuclear cells exposed to heat-killed Salmonella typhi.
abstract: Human mononuclear cells pre-labeled with [3H]arachidonic acid were shown to release metabolites following in vitro addition of heat-killed Salmonella typhi (HKST). The amount of label released was significantly higher than that seen with live S. typhi (LST). Addition of increasing amounts of HKST resulted in an increased release of metabolites. Enzyme immunoassay of the culture supernatants revealed that the bulk of the metabolite released was prostaglandin E2 (PGE2). Leukotriene B4 (LTB4) and leukotriene C4 (LTC4) were not detectable in the culture supernatants. The significance and implications of these results are discussed.
authors.affiliation: Institute of Advanced Studies, University of Malaya, Kuala Lumpur.
authors.forename: W N
authors.initials: WN
authors.lastname: Yusof
authors.forename: M
authors.initials: M
authors.lastname: Nagaratnam
authors.forename: C L
authors.initials: CL
authors.lastname: Koh
authors.forename: S
authors.initials: S
authors.lastname: Puthucheary
authors.forename: T
authors.initials: T
authors.lastname: Pang
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008954:Models, Biological; D009000:Monocytes / Q000378:metabolism*; D012485:Salmonella typhi; D015054:Zymosan / Q000494:pharmacology
chemical_list: D016718:Arachidonic Acid; D015054:Zymosan; D015232:Dinoprostone