The sugar beet mitochondrial genome contains an ORF sharing sequence homology with the gene for the 30 kDa subunit of bovine mitochondrial complex I.
From a sugar beet mitochondrial DNA library, we have isolated an open reading frame (ORF192) showing extensive homology to the gene for the 30 kDa subunit of the bovine mitochondrial complex I (NADH: ubiquinone reductase). The ORF192 was found to be actively transcribed to give an RNA of approximately 1.0 kb. We have designated this gene nad9. Transcripts from the nad9 locus are edited by five C to U transitions, increasing similarity with the amino acid sequence of the corresponding bovine and Neurospora crassa polypeptides. Southern blot hybridization also indicates that nad9 is present in the mitochondrial genomes of a variety of higher plant species.
Kubo
T
T
Plant Breeding Institute, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Mikami
T
T
Kinoshita
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004272:DNA, Mitochondrial / Q000235:genetics*; D042967:Electron Transport Complex I; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014675:Vegetables
D004272:DNA, Mitochondrial; D010940:Plant Proteins; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I