title: A cross-sectional study on osteocalcin levels in gingival crevicular fluid from periodontal patients.
abstract: The purpose of the present study was to determine the levels of osteocalcin, a bone specific matrix protein, in gingival crevicular fluid (GCF) from periodontal disease patients and to investigate the relationship between GCF osteocalcin levels and clinical parameters. Nineteen initial visit patients, 5 patients with gingivitis and 14 patients with adult periodontitis, participated in this study. The clinical parameters including probing depth, attachment level, gingival index, and tooth mobility were recorded following careful sampling of GCF with a filter paper strip harvested for 3 minutes. Osteocalcin adsorbed on a strip was extracted in a plastic tube containing 150 microliters of 10 mM sodium phosphate buffer (pH 6.5). GCF osteocalcin was determined by a newly-developed, high sensitive enzyme immunoassay which could recognize the N-terminal 20 residue peptide. In gingivitis patients, no significant amounts of osteocalcin were detected. In periodontitis patients, on the other hand, osteocalcin levels were detected, ranging between 0 and 540 pg/tube and positively correlated with these clinical parameters (P < 0.01). Moreover, in several sites in GI = 3 group, extremely higher levels of GCF osteocalcin were detected. These results strongly suggest that in addition to the presence of GCF osteocalcin the levels of osteocalcin may reflect the degree of the periodontal inflammation at the sampled sites.
authors.affiliation: Department of Periodontology, Nagasaki University School of Dentistry, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kunimatsu
authors.forename: S
authors.initials: S
authors.lastname: Mataki
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Mine
authors.forename: M
authors.initials: M
authors.lastname: Kiyoki
authors.forename: K
authors.initials: K
authors.lastname: Hosoda
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: I
authors.initials: I
authors.lastname: Kato
mesh_terms: D000293:Adolescent; D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005888:Gingival Pocket / Q000378:metabolism / Q000473:pathology; D005891:Gingivitis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D015675:Osteocalcin / Q000032:analysis*; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000378:metabolism / Q000473:pathology; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology; D014086:Tooth Mobility / Q000378:metabolism / Q000473:pathology
chemical_list: D015675:Osteocalcin