title: Ion content and cell volume in isolated collecting duct cells: effect of hypotonicity.
abstract: On isolated inner medullary collecting duct (IMCD) cells of the rat kidney the capability of osmoregulatory adaptation was investigated in vitro. IMCD cells were isolated by differential centrifugation at 600 mOsm (268 mM NaCl) and subsequently exposed to hypotonic buffers (300 mOsm, 118 mM NaCl). The alterations of ion content and cell volume following this change in extracellular osmolarity were studied by electron probe microanalysis and determination of intracellular water. After swelling within 40 seconds to 152 +/- 15% of control (P < 0.001; N = 9) cell volume was restored after 15 minutes. This regulatory volume decrease (RVD) was observed irrespective whether extracellular osmolarity was changed by using NaCl or mannitol as the major osmolyte. During RVD the cells lost sodium (48 +/- 11%) and chloride (14 +/- 5%), and the potassium content remained nearly unchanged. Correspondingly, sodium and chloride concentrations were progressively lowered, whereas the potassium concentration changed only transiently. RVD was diminished by 10(-4) M NPPB, 10(-3) M SITS and in the absence of HCO3-. Twenty millimoles of ouabain or 5 mM barium also inhibited RVD with little additive effect. A total of 10(-3) M amiloride and 10(-4) M bumetanide showed no effect on the hypoosmotic volume response. The experiments show that in isolated IMCD cells exposed to hypotonic conditions, rapid reversible changes in cell volume and sustained alterations in cell inorganic ion content occur, and thereby transmembrane sodium and potassium gradients are maintained. Since the loss in inorganic electrolytes does not account for RVD, the major part of volume regulation seems to occur via changes in organic osmolytes.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Max-Planck-Institut für molekulare Physiologie, Dortmund, Germany.
authors.forename: J M
authors.initials: JM
authors.lastname: Grunewald
authors.forename: R W
authors.initials: RW
authors.lastname: Grunewald
authors.forename: R K
authors.initials: RK
authors.lastname: Kinne
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D048429:Cell Size / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism; D007038:Hypotonic Solutions; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D014882:Water-Electrolyte Balance
chemical_list: D002712:Chlorides; D007038:Hypotonic Solutions; D001464:Barium; D010042:Ouabain; D012964:Sodium; D011188:Potassium