Comparison of the kinetic properties of the pyruvate dehydrogenase complex from pig kidney cortex and medulla.
The activity of the pyruvate dehydrogenase complex (PDC) purified from pig kidney medulla was affected by K+, Na+, Cl-, HCO3-, HPO4(2-) and changes in ionic strength. Increased ionic strength influenced the activity of PDC from medulla by decreasing the Vmax and S0.5 for pyruvate and increasing the Hill coefficient. The magnitude of these changes was smaller than the corresponding changes for PDC purified from the cortex. In the presence of K+ (80 mM), Na+ (20 mM), Cl- (20 mM), HCO3- (20 mM), HPO4(2-) (10 mM) and at ionic strength of 0.15 M the S0.5 for pyruvate of PDC from medulla was 117 microM and the enzyme complex was saturated by 1.1 mM pyruvate. Under these conditions the S0.5 for pyruvate of PDC derived from cortex was 159 microM and the enzyme was saturated at 4.5 mM pyruvate. Based on the results presented in this report it is suggested that PDC in kidney medulla may be regulated not only by a phosphorylation/dephosphorylation system and end-product inhibition but also via changes in ionic strength.
Pawełczyk
T
T
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284-7760.
Olson
M S
MS
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007477:Ions; D007672:Kidney Cortex / Q000201:enzymology*; D007679:Kidney Medulla / Q000201:enzymology*; D007700:Kinetics; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D010757:Phosphorous Acids / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism* / Q000502:physiology; D012680:Sensitivity and Specificity; D012964:Sodium / Q000494:pharmacology; D013552:Swine
D001639:Bicarbonates; D002712:Chlorides; D007477:Ions; D046911:Macromolecular Substances; D010757:Phosphorous Acids; D011768:Pyruvate Dehydrogenase Complex; D012964:Sodium; D011188:Potassium