Activity gel and activity blotting methods for detecting DNA-modifying (repair) enzymes.
Zymographical methods (activity gel, overlay gel, activity blot and activity blotting) for detecting DNA-modifying (repair) enzymes are reviewed. Emphasis is put on the novel activity blotting method in which DNA repair enzymes electrophoresed on a gel are blotted and detected on a damaged DNA-fixed nylon membrane. Its practical procedures, including a non-radioactive detection procedure, and representative results are also described.
Seki
S
S
Department of Molecular Biology, Okayama University Medical School, Japan.
Akiyama
K
K
Watanabe
S
S
Tsutsui
K
K
D000818:Animals; D011088:DNA Ligases / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D015151:Immunoblotting / Q000379:methods
D011088:DNA Ligases