Reduced binding capacity of concanavalin A-sepharose after treatment with chaotropic agents.
Affinity chromatography on Concanavalin A-Sepharose is widely used for purification of glycoproteins carrying mannose and glucose oligosaccharide moieties. High concentrations of chaotropes elute Concanavalin A from the gel matrix and significantly reduce the binding capacity of the column.
Fountoulakis
M
M
F. Hoffmann-La Roche Ltd., Pharmaceutical Research-New Technologies, Department of Biology, Basel, Switzerland.
Juranville
J F
JF
D000818:Animals; D002021:Buffers; D002846:Chromatography, Affinity / Q000379:methods; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D017830:Octoxynol / Q000494:pharmacology; D011489:Protein Denaturation; D012685:Sepharose / Q000031:analogs & derivatives* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D002021:Buffers; D006146:Guanidines; C028326:concanavalin A-sepharose; D012967:Sodium Dodecyl Sulfate; D007371:Interferon-gamma; D014508:Urea; D017830:Octoxynol; D012685:Sepharose; D019791:Guanidine