32P-postlabelling in studies of hormonal carcinogenesis.
The nature of the covalent modification of DNA by estrogens has been investigated by 32P-postlabelling and other techniques. Three different classes of covalent DNA alteration are induced by estrogens in vivo and are evaluated in this review for their potential to play a role in hormonal carcinogenesis: (a) estrogen-DNA adducts formed by reactive estrogen quinone metabolites; (b) enhancement of endogenous DNA modification by estrogen specifically in the target organ for cancer; (c) 8-hydroxyguanine bases formed by free radicals generated by redox cycling of estrogen. Additional studies are required to identify the class or classes of DNA adducts that are crucial for the induction of hormonal cancer.
Liehr
J G
JG
Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77555-1031.
Han
X
X
Bhat
H K
HK
D000818:Animals; D001665:Binding Sites; D006224:Cricetinae; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D005609:Free Radicals; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced* / Q000378:metabolism; D010761:Phosphorus Radioisotopes
D004273:DNA, Neoplasm; D004967:Estrogens; D005609:Free Radicals; D010761:Phosphorus Radioisotopes