Direct enantiomeric separation of cis-(+/-)diltiazem in plasma by high-performance liquid chromatography with ovomucoid column.
A method is proposed for the direct enantiomeric separation of cis-(+/-)diltiazem in plasma. The method is based on a commercial Ultron ES-OVM column with a mobile phase of different mixtures of ethanol-phosphate buffer. The degree of extraction of the enantiomers was more than 86%. The detection limit of the method used was 5 ng of racemic mixture on column (coefficient of variation = 13%), corresponding to 3.2 ng for cis-(+/-)diltiazem and 1.8 ng for cis-(+/-)diltiazem.
Rosell
G
G
Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Barcelona, Spain.
Camacho
A
A
Parra
P
P
D002851:Chromatography, High Pressure Liquid; D004110:Diltiazem / Q000097:blood* / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010059:Ovomucin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007202:Indicators and Reagents; D010059:Ovomucin; D000431:Ethanol; D004110:Diltiazem