Association constants of anti-hapten monoclonal IgG1 with mouse Fc gamma RII in the presence and absence of hapten.
The binding of five different mouse monoclonal anti-dinitrophenyl (DNP) IgGl monomers, in the presence and absence of DNP-glycine, to Fc gamma RII on the mouse macrophage-like cell line J774 has been investigated. Membrane association constants were estimated using a competitive radioimmunoassay in which the ability of the IgG1 monomers to block the binding of 125I-labelled Fabs of the anti-Fc gamma RII monoclonal antibody 2.4G2 was monitored. The IgG1-Fc gamma RII association constants ranged from (8 +/- 1) x 10(4) M-1 to (6 +/- 2) x 10(5) M-1. No large differences in the IgG1-Fc gamma RII association constants were measured for different IgG1 antibodies or for any single IgG1 antibody in the presence and absence of DNP-glycine.
Sumner
M T
MT
Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001666:Binding Sites, Antibody; D002460:Cell Line; D006241:Haptens / Q000276:immunology*; D006825:Hybridomas; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D051381:Rats; D017452:Receptors, IgG / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006241:Haptens; D007074:Immunoglobulin G; D017452:Receptors, IgG