Arabinan degrading enzymes from Aspergillus nidulans: induction and purification.
The presence in Aspergillus nidulans of two enzymes related to the Aspergillus niger endo-arabinase and alpha-L-arabinofuranosidase B has been established using antibodies against the purified A. niger enzymes. Moreover, the absence of an equivalent in A. nidulans to the alpha-L-arabinofuranosidase A of A. niger has been confirmed both at the protein and at the DNA level. Both A. nidulans arabinases have been purified and physico-chemically and kinetically characterized. They have a much higher temperature optimum than the corresponding A. niger enzymes. The pattern of induction has been studied on media containing different carbon sources showing an important role of L-arabitol in the induction of these enzymes.
Ramón
D
D
Unidad de Bioingeniería, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Valencia, Spain.
vd Veen
P
P
Visser
J
J
D001233:Aspergillus nidulans / Q000201:enzymology*; D001234:Aspergillus niger / Q000201:enzymology; D004790:Enzyme Induction; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
C074451:Endo-arabinase; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase