Mutational analysis of the bacteriophage P2 Ogr protein: truncation of the carboxy terminus.
The Ogr protein is a 72-residue, zinc-binding transcription factor essential for activation of late gene expression in bacteriophage P2. Analysis of C-terminal truncated proteins generated by stop codon mutagenesis shows that deletion of residues distal to position 51 had negligible effects on Ogr function. More-extensive deletion resulted in unstable products with severely reduced activity. These results, as well as the effects of other mutations in this region, support the idea that the 21 C-terminal residues are not required for transactivation.
Gebhardt
K
K
Institute of Biology, University of Oslo, Norway.
King
R A
RA
Christie
G E
GE
Lindqvist
B H
BH
D000595:Amino Acid Sequence; D017901:Bacteriophage P2 / Q000235:genetics*; D004252:DNA Mutational Analysis; D015967:Gene Expression Regulation, Viral; D016153:Genes, Suppressor; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D011994:Recombinant Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C074897:ogr protein, Enterobacteria phage P2