Dialkylformamidine protected deoxyadenosine phosphoramidites in oligonucleotide synthesis.
A series of dialkylformamidine protected deoxyadenosine phosphoramidites were prepared for automated, solid-support DNA synthesis. The set of Abz, Gdmf, Cbz, T phosphoramidites gave high purity and high yield oligonucleotides, with rapid and complete deprotection at 65 degrees C in one hour. Different times and temperatures of exposure to concentrated ammonium hydroxide were examined to establish the optimum conditions for deprotection of oligonucleotides.
Andrus
A
A
Division of Perkin-Elmer Co., Foster City, CA 94404.
Theisen
P D
PD
McCollum
C
C
D000578:Amidines / Q000737:chemistry*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003839:Deoxyadenosines / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D016133:Polymerase Chain Reaction
D000578:Amidines; D003839:Deoxyadenosines; D009841:Oligonucleotides; D009943:Organophosphorus Compounds