Mutants of Epstein-Barr virus with a selective marker disrupting the TP gene transform B cells and replicate normally in culture.
We have isolated mutants of Epstein-Barr virus (EBV) which carry a dominant selectable marker inserted into the third exon of the gene encoding two membrane proteins, TP1 and TP2 (or LMP2A and LMP2B), which are expressed in latently infected, growth-transformed B cells. One of the mutants also acquired a 260-bp deletion beginning in the first intron a few base pairs from the terminal repeats and removing most of the second TP exon, including the initial coding sequences of TP2. These EBV mutants transform human B cells in culture, and the transformed B-cell clones carrying them release EBV at approximately normal frequencies.
Kim
O J
OJ
Department of Human Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263-0001.
Yates
J L
JL
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D001402:B-Lymphocytes / Q000382:microbiology*; D001483:Base Sequence; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics*; D002999:Clone Cells; D005091:Exons / Q000235:genetics; D005819:Genetic Markers; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000254:growth & development; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012641:Selection, Genetic; D014763:Viral Matrix Proteins / Q000235:genetics*; D014776:Virus Cultivation; D014779:Virus Replication
D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D005819:Genetic Markers; D014763:Viral Matrix Proteins