Type II iodothyronine 5'-deiodinase in mouse brown adipose tissue is stimulated by a single injection of isoproterenol in an oil/water emulsion.
A single subcutaneous morning injection of the beta-adrenergic agonist isoproterenol (ISO) (10 mg/kg body weight) in an oil/water emulsion (70/30; v/v) caused a marked increase in the activity of the enzyme type II iodothyronine 5'-deiodinase (5'-D II) in the interscapular brown adipose tissue of BDF-1 mice. After a delay of 4 hours, the 5'-D II activity began to rise in an almost linear fashion and was increased 3-fold after 8 hours, when compared to the control values. The results indicate that this method of ISO administration may be a valid tool for artificial stimulation of brown adipose tissue in animals by beta-adrenergic agonists.
Lerchl
A
A
Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio 78284-7762.
Stokkan
K A
KA
Nonaka
K O
KO
Vaughan
M K
MK
Reiter
R J
RJ
D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D004655:Emulsions; D007453:Iodide Peroxidase / Q000378:metabolism*; D007545:Isoproterenol / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013268:Stimulation, Chemical
D004655:Emulsions; D007453:Iodide Peroxidase; D007545:Isoproterenol