title: Age-related changes in calcium homeostatic mechanisms in synaptosomes in relation with working memory deficiency.
abstract: Aging is associated with alterations in different systems that govern neuronal calcium homeostasis. This study was designed to determine whether any of these alterations may contribute to the decline in spatial working memory that is observed in old rats. Several parameters [initial (5 s) and steady state (15 min) 45Ca2+ uptake, FCCP-releaseable 45Ca2+, [Ca2+]i levels, depolarization-induced phosphoprotein (P97, PP65, P42) dephosphorylation and acetylcholine levels and release) involved in calcium homeostasis/signaling were determined in whole brain synaptosomes derived from adult (9-month-old) and old (24-month-old) rats that were evaluated for spatial memory performance in the eight-arm radial maze. The neurochemical analysis indicated that both the 9- and 24-month-old rats were impaired with respect to 3-month-old animals. When learners (animals reaching criterion; RC) were compared to memory impaired rats (MI), it was found that the FCCP-releaseable 45Ca2+ of synaptosomes, that reflects mitochondrial calcium, was lower in the MI than the RC rats and was correlated with the behavioral performance of the rats in their first testing sessions. The results suggest that the loss of calcium uptake capacity in synaptic mitochondria during aging may be associated with impaired working memory in old animals.
authors.affiliation: Department of Animal Learning Psychology, Faculty of Psychology, Complutense University of Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Huidobro
authors.forename: P
authors.initials: P
authors.lastname: Blanco
authors.forename: M
authors.initials: M
authors.lastname: Villalba
authors.forename: P
authors.initials: P
authors.lastname: Gómez-Puertas
authors.forename: A
authors.initials: A
authors.lastname: Villa
authors.forename: R
authors.initials: R
authors.lastname: Pereira
authors.forename: E
authors.initials: E
authors.lastname: Bogónez
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Serrano
authors.forename: J J
authors.initials: JJ
authors.lastname: Aparicio
authors.forename: J
authors.initials: J
authors.lastname: Satrústegui
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000375:Aging / Q000378:metabolism* / Q000523:psychology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D003600:Cytosol / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology*; D008297:Male; D008569:Memory Disorders / Q000378:metabolism*; D008570:Memory, Short-Term; D008928:Mitochondria / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D013028:Space Perception / Q000502:physiology; D013574:Synaptosomes / Q000378:metabolism*
chemical_list: D002132:Calcium Radioisotopes; D000109:Acetylcholine; D002118:Calcium