Immunomodulation due to coexposure to styrene and dioctyl phthalate in mice.
Pathomorphological and immunological alterations caused by a mixture of styrene and dioctyl phthalate were studied in albino mice following oral administration of 0.02, 0.03, 0.05 x LD50 of the mixture. The chemicals were mixed together proportionate to their respective LD50 values and fed in ground nut oil, 5 d/wk for 4 weeks. Histological examination of spleen revealed considerable depletion of cellular population of lymphoid follicles which corresponded to the dose dependent decrease in splenic mononuclear cell population count. The thymic lobules revealed slight atrophy but accompanied by a significant increase in thymocyte population. Correspondingly few significant histological changes were observed in mesenteric and peripheral lymph nodes. The treatment caused impairment of primary humoral immune response to SRBC (IgM) but there was a significant increase in response of splenocytes to B-cell mitogen LPS. There was a suppression of cutaneous delayed type hypersensitivity and increase in splenic lymphocyte response to T-cell mitogen PHA. Simultaneously, indirect immunity represented by decreased phagocytosis and enhanced metabolic function of reducing NBT by peritoneal exudate cells was observed. The in vitro exposure of vero cells to the mixture caused dose dependent protective effect. The results of present study indicate that subchronic exposure to low doses of mixture of styrene and dioctyl phthalate under certain conditions may modulate some of the immune functions as compared to exposure to either chemicals alone.
Dogra
R K
RK
Immunotoxicology Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
Khanna
S
S
Srivastava
S N
SN
Shukla
L J
LJ
Chandra
K
K
Saxena
G
G
Shanker
R
R
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D004357:Drug Synergism; D007107:Immune System / Q000187:drug effects*; D007111:Immunity, Cellular / Q000187:drug effects; D007928:Lethal Dose 50; D008221:Lymphoid Tissue / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D020058:Styrene; D013343:Styrenes / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D014709:Vero Cells
D000276:Adjuvants, Immunologic; D013343:Styrenes; D020058:Styrene; D004051:Diethylhexyl Phthalate