title: Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis.
abstract: We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12,700 bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidine-rich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.
authors.affiliation: Dipartimento di Biologia, Università di Roma Tor Vergata, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Mariottini
authors.forename: C
authors.initials: C
authors.lastname: Bagni
authors.forename: A
authors.initials: A
authors.lastname: Francesconi
authors.forename: F
authors.initials: F
authors.lastname: Cecconi
authors.forename: M J
authors.initials: MJ
authors.lastname: Serra
authors.forename: Q M
authors.initials: QM
authors.lastname: Chen
authors.forename: F
authors.initials: F
authors.lastname: Loreni
authors.forename: F
authors.initials: F
authors.lastname: Annesi
authors.forename: F
authors.initials: F
authors.lastname: Amaldi
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D005091:Exons; D007438:Introns; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D014982:Xenopus laevis / Q000235:genetics*
chemical_list: D012269:Ribosomal Proteins; C030254:ribosomal protein S8; D004247:DNA