Membrane damage induced in cultured human skin fibroblasts by UVA irradiation.
Irradiation of cultured human skin fibroblasts with ultraviolet light from 320 to 400 nm (UVA) leads to a decrease in the membrane fluidity exemplified by an enhanced fluorescence anisotropy of the lipophilic fluorescent probe 1-[4-trimethylamino)-phenyl]-6-phenylhexa-1,3,5-triene. This UVA-induced decrease in fluidity is associated with lactate dehydrogenase leakage in the supernatant. Vitamin E, an inhibitor of lipid peroxidation, exerts a protective effect on both phenomena. Therefore, this UVA-induced damage in membrane properties may be related to lipid peroxidation processes. Moreover, exponentially growing cells are more sensitive to these UVA-induced alterations than confluent cells.
Gaboriau
F
F
Laboratoire de Physique et Chimie Biomoléculaires, CNRS U.A. 198, Institut Curie, Paris, France.
Morlière
P
P
Marquis
I
I
Moysan
A
A
Gèze
M
M
Dubertret
L
L
D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000473:pathology / Q000528:radiation effects; D006801:Humans; D008560:Membrane Fluidity / Q000528:radiation effects*; D012867:Skin / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays