12-0-Tetradecanoylphorbol 13-acetate (TPA)-induced enzymatic change of human non-T, non-B lymphoid leukemia cell lines.
Culture of two lymphoid leukemia cell lines with 5 x 10(-9) to 10(-7) M 12-0-tetradecanoyl-phorbol 13-acetate (TPA) induced the significant increase in sialyltransferase, acid phosphatase and butyrate esterase activities, the decrease in poly(A) polymerase and terminal deoxynucleotidyl transferase (TdT) activities. B4-, J5-, TdT- or PNA-positive cells were decreased in TPA-treated cells, while B1-positive cells were increased. These results suggest enzymatic changes as an aspect of TPA-induced differentiation in lymphoid leukemia cell lines. These enzymatic activities may be useful markers for the differentiation of lymphoid leukemia cells.
Sasaki
R
R
First Department of Internal Medicine, Jichi Medical School, Tochigi, Japan.
Kubonishi
I
I
Minowada
J
J
Bollum
F J
FJ
Miura
Y
Y
Takaku
F
F
D002454:Cell Differentiation / Q000187:drug effects; D004253:DNA Nucleotidylexotransferase / Q000096:biosynthesis; D004257:DNA Polymerase II / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000201:enzymology; D011062:Polynucleotide Adenylyltransferase / Q000096:biosynthesis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D012799:Sialyltransferases / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D012799:Sialyltransferases; D011062:Polynucleotide Adenylyltransferase; D004253:DNA Nucleotidylexotransferase; D004257:DNA Polymerase II; D013755:Tetradecanoylphorbol Acetate