Natural occurrence of Nuc in the sera of autoimmune-prone MRL/lpr mice.
We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant(r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
Kanai
Y
Y
Department of Molecular Oncology, University of Tokyo, Japan.
Miura
K
K
Uehara
T
T
Amagai
M
M
Takeda
O
O
Tanuma
S
S
Kurosawa
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005803:Genes, Immunoglobulin / Q000187:drug effects; D006133:Growth Substances / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000145:classification; D007075:Immunoglobulin M / Q000096:biosynthesis; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000276:immunology
D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D006133:Growth Substances; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D011994:Recombinant Proteins