16 kDa envelope proteins in non-Serpulina hyodysenteriae spirochaetes isolated from pigs.
Spirochaetes isolated from field samples of diarrhoea, 'colitis' and mucoid diarrhoea from pigs were examined by a series of cultural, biochemical and serological tests. In addition sodium dodecyl sulphate polyacrylamide gel electrophoresis was used to determine whether the organisms possessed a 16 kDa protein thought to distinguish Serpulina hyodysenteriae from S innocens. Spirochaetal isolates which differed culturally and biochemically from S hyodysenteriae were found to possess a 16 kDa protein. One of these isolates was examined by electron microscopy and found to have an ultrastructure differing from that of S hyodysenteriae. Antiserum to the 16 kDa antigen of S hyodysenteriae reacted with isolate S80/5, the homologous strain, and with B78, the type species, but not with the 16 kDa antigens of the field isolates considered to be S hyodysenteriae or with the non-S hyodysenteriae spirochaetes. It was concluded that there may be a family of 16 kDa proteins located on the envelope of various spirochaetes responsible for diarrhoea in pigs.
Thom
F
F
Department of Pathology, University of Glasgow Veterinary School, Bearsden.
Taylor
D J
DJ
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D017400:Brachyspira / Q000737:chemistry* / Q000648:ultrastructure; D017401:Brachyspira hyodysenteriae / Q000737:chemistry / Q000648:ultrastructure; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D006801:Humans; D007841:Latex Fixation Tests / Q000662:veterinary; D008854:Microscopy, Electron / Q000662:veterinary; D013142:Spirochaeta / Q000254:growth & development / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D001425:Bacterial Outer Membrane Proteins