title: Effect of membrane phospholipid composition and fluidity on rat liver plasma membrane tyrosine kinase activity.
abstract: 1. The effect of membrane phospholipid composition and fluidity on tyrosine kinase activity was investigated in rat liver plasma membranes. 2. The phospholipid composition has been modified by in vitro enrichment of plasma membranes with different phospholipids in the presence of lipid transfer proteins and by partial delipidation with exogenous phospholipases A2, C and D and subsequent enrichment with phosphatidylglycerol. 3. Phosphatidylglycerol and dioleoylglycerophosphocholine caused dramatic elevation of this activity, while phosphatidylserine and phosphatidylethanolamine were less effective. Enrichment with dipalmitoylglycerophosphocholine and sphingomyeline reduced tyrosine kinase activity.
authors.affiliation: Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
authors.forename: N J
authors.initials: NJ
authors.lastname: Gavrilova
authors.forename: M S
authors.initials: MS
authors.lastname: Setchenska
authors.forename: T T
authors.initials: TT
authors.lastname: Markovska
authors.forename: A B
authors.initials: AB
authors.lastname: Momchilova-Pankova
authors.forename: K S
authors.initials: KS
authors.lastname: Koumanov
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology*; D008563:Membrane Lipids / Q000032:analysis*; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008563:Membrane Lipids; D010743:Phospholipids; D011505:Protein-Tyrosine Kinases; D010740:Phospholipases