title: Secretagogue-induced calcium wave shows higher and prolonged transients of nuclear calcium concentration in mast cells.
abstract: To clarify the mechanism of secretagogue (compound 48/80)-induced calcium signaling in rat peritoneal mast cells, we analyzed serial confocal calcium images with high spatial and temporal resolution using different Ca(2+)-probes. The Ca(2+)-wave began at the periphery of the cytoplasm, and then spread to the center of the nucleus. Nuclear [Ca2+]i was clearly higher than cytoplasmic [Ca2+]i. The heterogeneity of [Ca2+]i continued until about 2 min after degranulation. These results suggest the existence of an intranuclear Ca(2+)-store which possesses a Ca(2+)-releasing mechanism similar to that in the cytoplasm.
authors.affiliation: Department of Pathology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Katagiri
authors.forename: T
authors.initials: T
authors.lastname: Takamatsu
authors.forename: T
authors.initials: T
authors.lastname: Minamikawa
authors.forename: S
authors.initials: S
authors.lastname: Fujita
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D016257:Fura-2; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D008853:Microscopy / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
chemical_list: D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium; D016257:Fura-2