Macrophage progenitors from mouse bone marrow and spleen differ in their expression of the Ly-6C differentiation antigen.
Using mAb and magnetic and fluorescence-activated cell sorting, together with culturing in semisolid culture medium, we show that essentially all splenic macrophage progenitors are Ly-6ChiMac-1-. By contrast, bone marrow macrophage progenitors were more heterogeneous in Ly-6C expression, the majority being restricted to the Ly-6CdimMac-1- and the Ly-6C-Mac-1- subsets. These findings provide immunophenotype evidence that macrophages in the spleen and bone marrow arise from different progenitors.
McCormack
J M
JM
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 37105.
Leenen
P J
PJ
Walker
W S
WS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000950:Antigens, Ly / Q000032:analysis*; D001483:Base Sequence; D001854:Bone Marrow Cells; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D016177:Macrophage-1 Antigen / Q000032:analysis; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D015704:CD4 Antigens; D016827:CD8 Antigens; D016177:Macrophage-1 Antigen