title: Expression and localization of intestinal 15 kDa protein in the rat.
abstract: Rat intestinal 15 kDa protein (I-15P) is highly homologous to porcine gastrotropin. We studied the occurrence, distribution and subcellular localization of I-15P in the entire rat body, using the immunocytochemistry to localize protein and in situ hybridization to localize mRNA. Both techniques demonstrated the expression of I-15P in the enterocytes of ileum, luteal cells of ovary and a subpopulation of steroid-endocrine cells of adrenal gland. Immuno-electron microscopy further demonstrated that I-15P is localized in both the cytoplasmic and nuclear matrix regions of these cells. The present results suggest roles of I-15P not only in the transport of bile salts but also in the metabolisms of certain steroid hormones.
authors.affiliation: Department of Anatomy, School of Medicine, Kanazawa University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Iseki
authors.forename: O
authors.initials: O
authors.lastname: Amano
authors.forename: T
authors.initials: T
authors.lastname: Kanda
authors.forename: H
authors.initials: H
authors.lastname: Fujii
authors.forename: T
authors.initials: T
authors.lastname: Ono
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis*; D015342:DNA Probes; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005260:Female; D005768:Gastrointestinal Hormones; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D002352:Carrier Proteins; D015342:DNA Probes; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005768:Gastrointestinal Hormones; C057275:fatty acid-binding protein 6