Comparison of methods for specific depletion of ATP in Salmonella typhimurium.
Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or alpha-methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP-dependent processes from processes dependent on other nucleotides.
Johnson
M S
MS
Department of Microbiology, Loma Linda University School of Medicine, California 92350.
Taylor
B L
BL
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001149:Arsenates / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D001431:Bacteriological Techniques; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008757:Methylglucosides / Q000494:pharmacology; D009154:Mutation; D009711:Nucleotides / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D019810:Sodium Azide
D001149:Arsenates; D001386:Azides; D008757:Methylglucosides; D009711:Nucleotides; D006639:Histidine; C027020:methylglucoside; D000255:Adenosine Triphosphate; D019810:Sodium Azide; C025657:arsenic acid