title: Effect of constant administration of a gonadotropin-releasing hormone agonist on reproductive activity in mares: induction of ovulation during seasonal anestrus.
abstract: The potential of a gonadotropin-releasing hormone (GnRH) agonist (goserelin acetate), delivered constantly for 28 days via a subcutaneous depot, to induce ovulation in seasonally anestrous mares, was investigated. Two experiments were conducted, in which a range of doses (30 to 240 micrograms/mare/d) was examined. Mares were selected on the basis of lack of substantial follicular development (follicle diameter < 20 mm determined ultrasonically) and low serum concentrations of luteinizing hormone (LH) and progesterone. Constant administration of the GnRH agonist-induced ovulation in anestrous mares, but a dose-response relation was not observed. Furthermore, with identical doses tested in consecutive or alternate years, considerable variation was observed in the ovulatory response. In general, ovulation in all treated mares was accompanied by increased circulating concentrations of LH and a decrease in follicle-stimulating hormone values. Ovulation was preceded by an increase in estradiol and LH concentrations. In mares in which ovulation did not occur, concentration of LH increased during agonist treatment, whereas that of follicle-stimulating hormone either increased or did not change. It was concluded that constant administration of GnRH agonists may induce ovulation in mares during seasonal anestrus; however, percentage of mares ovulating and the lack of reproducibility of effect indicate that this approach is inappropriate for use as a reliable method to manipulate breeding activity in commercial broodmares.
authors.affiliation: Department of Veterinary Science, Maxwell Gluck Equine Research Center, University of Kentucky, Lexington 40546-0099.
authors.forename: B P
authors.initials: BP
authors.lastname: Fitzgerald
authors.forename: S L
authors.initials: SL
authors.lastname: Meyer
authors.forename: K J
authors.initials: KJ
authors.lastname: Affleck
authors.forename: P J
authors.initials: PJ
authors.lastname: Silvia
mesh_terms: D000780:Anestrus / Q000187:drug effects*; D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D006065:Gonadotropins, Pituitary / Q000097:blood; D017273:Goserelin / Q000494:pharmacology*; D006736:Horses / Q000097:blood / Q000502:physiology*; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D011374:Progesterone / Q000097:blood
chemical_list: D006065:Gonadotropins, Pituitary; D017273:Goserelin; D011374:Progesterone; D004958:Estradiol