[The effect of oxygen supply in continuous cold perfusion of the rat liver using perfluorochemical emulsion--parenchymal and nonparenchymal cell injuries evaluated with trypan blue perfusion/fixation techniques].
We investigated the effect of hypothermic aerobic perfusion of the rat liver using perfluoro-N-methyl-decahydroisoquinoline (FMIQ) on the cell viabilities of hepatic parenchyma and nonparenchyma in comparison with University of Wisconsin (UW) solution. Rat liver were perfused at 4 degrees C up to 24hr at a flow rate of 0.2 ml/g liver tissue/min with either UW or a perfusate (m-FMIQ) containing 20% FMIQ with or without oxygen saturation. After storage, livers were perfused with Krebs-Henseleit buffer containing trypan blue (200 microM). Nuclear dye uptake was measured as an indicator of cell death. Dye uptake of parenchymal cells remained less than 2% for up to 24hr, regardless of perfusate or O2-saturation. In UW perfused livers, nonparenchymal cells showed time related increases of dye positive nuclei, which were not affected by O2-saturation (39.9 +/- 4.7% and 36.5 +/- 4.2% at 24hr in O2-saturated and nonsaturated groups, respectively). In contrast, we found excellent protective effects of oxygen supply with m-EMIQ on the nonparenchymal cell viability, the dye uptake values being 16.9 +/- 5.7% and 39.4 +/- 9.1% at 24hr in O2-saturated and nonsaturated groups, respectively (p < 0.001). In conclusion, oxygen supply during hypothermic perfusion may be useful in preventing nonparenchymal cell injury of the liver, thereby leading to dramatic improvement of the hepatic microcirculation.
Shiki
H
H
First Department of Surgery, Kobe University School of Medicine, Japan.
Ku
Y
Y
Kuroda
Y
Y
Saito
Y
Y
D000241:Adenosine / Q000008:administration & dosage; D000493:Allopurinol / Q000008:administration & dosage; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003080:Cold Temperature; D005466:Fluorocarbons / Q000008:administration & dosage* / Q000494:pharmacology; D005978:Glutathione / Q000008:administration & dosage; D066298:In Vitro Techniques; D007328:Insulin / Q000008:administration & dosage; D007546:Isoquinolines / Q000008:administration & dosage* / Q000494:pharmacology; D008099:Liver / Q000166:cytology*; D008297:Male; D019188:Organ Preservation Solutions; D010100:Oxygen / Q000378:metabolism*; D010477:Perfusion; D011887:Raffinose / Q000008:administration & dosage; D051381:Rats; D011919:Rats, Inbred Strains; D016707:Tissue Fixation / Q000379:methods*; D014343:Trypan Blue
D005466:Fluorocarbons; D007328:Insulin; D007546:Isoquinolines; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; C065081:perfluoro-N-methyldecahydroisoquinoline; D005978:Glutathione; D014343:Trypan Blue; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen