Comparison of a cold enrichment method and the IDF method for isolation of Listeria monocytogenes from animal autopsy material.
The method most often used in Sweden for isolation of Listeria monocytogenes from animal autopsy material is a cold enrichment method. This method is very slow. The International Dairy Federation (IDF) has recently presented a method for detection of L. monocytogenes in milk and milk products that is complete in one week. During a two year period 69 specimens from dead animals with suspected listeriosis were examined for L. monocytogenes in parallel analyses with both the cold enrichment method and the IDF method. Samples derived from different autopsy material representing a variety of animals. L. monocytogenes was isolated in 27.5% of the samples with the IDF method but only in 4.3% with the cold enrichment method. It is concluded that the IDF method was more sensitive than the cold enrichment method.
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Bacteriological Laboratory, National Veterinary Institute, Uppsala, Sweden.
Danielsson-Tham
M L
ML
Gunnarsson
A
A
Tham
W
W
D000818:Animals; D003080:Cold Temperature; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008088:Listeriosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*