The sarco(endo)plasmic reticulum Ca(2+)-ATPase mRNA isoform, SERCA 3, is expressed in endothelial and epithelial cells in various organs.
The sarco(endo)plasmic reticulum Ca(2+)-ATPase mRNA isoform, SERCA 3, was previously shown to be expressed in a great variety of muscle and non-muscle tissues [(1989) J. Biol. Chem. 264, 18568] but its cellular localization within these organs was unknown. We have used in situ hybridization and RNase protection techniques to demonstrate that SERCA 3 mRNA is expressed in specific cell types, namely the endothelial and epithelial cells.
Anger
M
M
INSERM U 275-LOA-ENSTA, Ecole Polytechnique, Centre de l'Yvette, Palaiseau, France.
Samuel
J L
JL
Marotte
F
F
Wuytack
F
F
Rappaport
L
L
Lompré
A M
AM
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D017403:In Situ Hybridization; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D012260:Ribonucleases; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
D012333:RNA, Messenger; D012260:Ribonucleases; D000252:Calcium-Transporting ATPases