title: Importance of specific purine-pyrimidine amino and hydroxyl groups for efficient cleavage by a hammerhead ribozyme.
abstract: Subsequent of the discovery that RNA can have site specific cleavage activity, there has been a great deal of interest in the design and testing of trans-acting catalytic RNAs as therapeutic agents. We have chemically synthesized and experimentally analyzed chimeric DNA-RNA ribozymes in which the catalytic center remains as RNA.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Hosaka
authors.forename: R
authors.initials: R
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Imamura
authors.forename: K
authors.initials: K
authors.lastname: Takai
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: H
authors.initials: H
authors.lastname: Takaku
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011687:Purines / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011687:Purines; D011743:Pyrimidines; D016337:RNA, Catalytic; D017665:Hydroxyl Radical