Phenotypic alteration of glioma cells during culture.
Tissue specimens and culture cells from three human gliomas (two astrocytomas and one glioblastoma) were immunohistochemically investigated, using GFAP, S-100P, vimentin, FN and TNF antibodies. Primary culture consisted of two cell types, flat cells and fibrous cells. Phenotypic alternation was observed during successive subculture. Differences between fibrous cells in astrocytoma and those in glioblastoma were remarkable, while flat cells in astrocytoma and glioblastoma examined in this study, were similar.
Imai
F
F
Department of Neurosurgery, School of Medicine, Fujita Health University.
Marunouchi
T
T
Mizoguchi
Y
Y
Ogasawara
M
M
Kanno
T
T
D000328:Adult; D001254:Astrocytoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002648:Child; D005353:Fibronectins / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010641:Phenotype; D009418:S100 Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D005353:Fibronectins; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; D014409:Tumor Necrosis Factor-alpha; D014746:Vimentin