title: Regulation of glycolytically fueled Ca2+ uptake in smooth muscle plasmalemmal vesicles by phosphorylation.
abstract: A highly purified plasma membrane vesicular preparation from porcine antrum had an endogenous protein kinase activity with substrates of molecular weights of 11, 15, 20.5, 25, 35, 44, 155, and 230 x 10(3). Phosphorylation of the plasma membranes by the endogenous protein kinase activity resulted in a stimulation of initial rates of Ca2+ uptake into inside-out vesicles, which was associated with an increase in the maximum velocity of the Ca2+ pump with no apparent changes in the half-maximal effective concentration for calcium. Because we have previously reported that a membrane-associated glycolytic system may preferentially provide ATP to fuel the Ca2+ pump (9), we examined the effects of phosphorylation on Ca2+ uptake when glycolysis was the sole source of ATP for the pump. We found that the stimulation of Ca2+ uptake by phosphorylation was more pronounced when Ca2+ uptake was supported by glycolysis rather than 2 mM ATP. When ATP was added at a level similar to that produced by endogenous glycolysis, the stimulation of Ca2+ uptake by phosphorylation was comparable to when glycolysis supported the Ca2+ pump. Our observations suggest that the dynamic range (up to threefold) for regulation of the plasmalemmal Ca2+ pump by phosphorylation is considerably larger than previously reported and thus likely to be of physiological significance.
authors.affiliation: Department of Physiology and Biophysics, University of Cincinnati College of Medicine, Ohio 45267-0576.
authors.forename: C D
authors.initials: CD
authors.lastname: Hardin
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: E G
authors.initials: EG
authors.lastname: Kranias
authors.forename: N A
authors.initials: NA
authors.lastname: Steenaart
authors.forename: L
authors.initials: L
authors.lastname: Raeymaekers
authors.forename: R J
authors.initials: RJ
authors.lastname: Paul
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D006019:Glycolysis; D009130:Muscle, Smooth / Q000378:metabolism*; D010766:Phosphorylation; D013552:Swine
chemical_list: D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases; D002118:Calcium