Measurement of oxygen-derived free radical generation in the regionally-ischemic rat heart by the chemiluminescence method.
Generation of oxygen-derived free radicals (oxy-radicals) in the stored rat heart was measured by chemiluminescence. Hearts subjected (ischemia) or not subjected (non-ischemia) to a 4-min regional ischemia were frozen in liquid nitrogen and stored until assayed. The frozen myocardium was ground and oxygenated by mixing it with phosphate-buffered saline (Po2: 194 mmHg) containing lucigenin. The chemiluminescence intensity of ischemic myocardium was larger than that of non-ischemic myocardium. Recombinant human superoxide dismutase significantly decreased these intensities. These results indicate that O2- is one of the major oxy-radical species in the rat heart and that the generation of oxyradicals is enhanced by regional ischemia for 4 min.
Ohoi
I
I
Pharmacology Section, Nippon Kayaku Co., Ltd., Tokyo, Japan.
Tobari
H
H
Nakamura
K
K
D000166:Acridines / Q000494:pharmacology; D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology
D000166:Acridines; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; C033472:10,10'-dimethyl-9,9'-biacridinium; D013482:Superoxide Dismutase