title: Preparation of chromosome-specific paints and complete assignment of chromosomes in the pig flow karyotype.
abstract: Sorted chromosomes from each of the 20 clusters of the male porcine bivariate flow karyotype were amplified and biotinylated using DOP-PCR. The chromosomes comprising each cluster were identified by fluorescence in situ hybridization (FISH) of the 20 probes to R-banded male pig metaphase spreads. A standard flow karyotype for the pig is presented.
authors.affiliation: AFRC Institute of Animal Physiology & Genetics Research, Babraham Research Station, Babraham, UK.
authors.forename: C F
authors.initials: CF
authors.lastname: Langford
authors.forename: H
authors.initials: H
authors.lastname: Telenius
authors.forename: N G
authors.initials: NG
authors.lastname: Miller
authors.forename: P D
authors.initials: PD
authors.lastname: Thomsen
authors.forename: E M
authors.initials: EM
authors.lastname: Tucker
mesh_terms: D000818:Animals; D001483:Base Sequence; D001710:Biotin; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure; D017931:DNA Primers; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005456:Fluorescent Dyes; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary*; D007621:Karyotyping / Q000662:veterinary*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D001710:Biotin