title: New strategies for oligonucleotide synthesis by use of 2-trimethylsilylethyl and 2-trimethylsilylethoxymethyl as the phosphate and 2'-hydroxyl protecting groups, respectively.
abstract: The 2-Trimethylsilylethyl (TSE) group was found to be useful as a protecting group for the internucleotidic phosphate in DNA synthesis. Oligodeoxyribonucleotides were synthesized in good yields by using phosphoramidite building blocks having the TSE group. In RNA synthesis, 2-trimethylsilylethoxymethyl (SEM) group was introduced as a new protecting group for the 2'-hydroxyl. Selective protection of this function with SEM-Cl was achieved in good yield by use of 3',5'-O-bis(tert-butyl)silanediyl ribonucleoside derivatives.
authors.affiliation: Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Wada
authors.forename: M
authors.initials: M
authors.lastname: Tobe
authors.forename: T
authors.initials: T
authors.lastname: Nagayama
authors.forename: K
authors.initials: K
authors.lastname: Furusawa
authors.forename: M
authors.initials: M
authors.lastname: Sekine
mesh_terms: D006900:Hydroxylation; D009841:Oligonucleotides / Q000138:chemical synthesis*; D014297:Trimethylsilyl Compounds / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D014297:Trimethylsilyl Compounds