title: CD23 expression on B-lymphocytes and its modulation by cytokines in allergic patients.
abstract: The aim of this study was to assess the expression of CD23 on peripheral blood B-cells, and its in vitro modulation by recombinant human interferon-gamma (IFN-gamma) in phytohaemagglutinin-(PHA) or recombinant human interleukin-4 (IL-4)-stimulated cultures in atopic patients with Dermatophagoides pteronyssinus hypersensitivity and in healthy non-atopic subjects. Atopic patients with asthma not receiving allergen-specific immunotherapy (n = 21) were studied and further compared with a group of atopic subjects with asthma under allergen-specific immunotherapy (n = 21). They were age-(+/- 5 yr) and sex-matched. The results were also compared with those obtained in the non-atopic group (n = 11). CD23 expression on B-lymphocytes and its modulation were analyzed by flow cytometry using conjugated monoclonal antibodies with a double immunofluorescence method. Atopic patients had an increase in the percentage of B-cells expressing CD23 in peripheral blood. Phytohaemagglutinin and IL-4 induced a rise in the percentage of CD23-positive B-cells in both atopic groups and non-atopic subjects. Phytohaemagglutinin provoked an increase in the intensity of CD23 expression on B-cells from stimulated cultures in all groups, while IL-4 only produced a significant increase in atopic patients. The presence of IFN-gamma decreased the CD23 expression on B-cells in PHA-stimulated culture of atopic patients, whereas it caused an increase in CD23 expression in the non-atopic group. Furthermore, the presence of IFN-gamma in IL-4-stimulated cultures induced a decrease in CD23 expression on B-cells in all cases.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Immunology Service, Hospital Duran i Reynals, Ciutat Sanitària de Bellvitge, L'Hospitalet de Llobregat Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Corominas
authors.forename: M
authors.initials: M
authors.lastname: Mestre
authors.forename: J
authors.initials: J
authors.lastname: Bas
authors.forename: J
authors.initials: J
authors.lastname: Verdaguer
authors.forename: A
authors.initials: A
authors.lastname: Valls
authors.forename: A
authors.initials: A
authors.lastname: Romeu
authors.forename: E
authors.initials: E
authors.lastname: Buendia
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000276:immunology* / Q000628:therapy; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000276:immunology; D003888:Desensitization, Immunologic; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology; D017455:Receptors, IgE / Q000096:biosynthesis*
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D016207:Cytokines; D005938:Glucocorticoids; D006023:Glycoproteins; D017455:Receptors, IgE