Cloning and structural analysis of a human thymidylate synthase pseudogene splitted by several Alu sequences.
An unidentified genomic DNA fragment of 2.4kb that is weakly hybridizable with thymidylate synthase (TS) cDNA was cloned from a human genomic DNA library. Sequencing of the cloned DNA fragment and comparison of the sequence with that of the known human TS cDNA revealed that the DNA fragment contained a human TS processed pseudogene with unusual features. Based on the rate of nucleotide substitutions for neutral mutations in the 3'-untranslated regions between the gene and the pseudogene, it was estimated that the human TS pseudogene was formed about 16 million years ago.
Takeishi
K
K
Laboratory of Genetic Engineering, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Watanabe
Y
Y
Isono
K
K
Horie
N
N
D005075:Biological Evolution; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D011544:Pseudogenes; D012091:Repetitive Sequences, Nucleic Acid; D013940:Thymidylate Synthase / Q000235:genetics*
D018076:DNA, Complementary; D013940:Thymidylate Synthase