Comparative study of staphylococcal co-agglutination and latex agglutination for detection of enterotoxigenic Escherichia coli.
Several biological and immunological methods have been used for detection of ETEC. In this work a comparative study was done between staph-co-agglutination and latex agglutination. From the 75 faecal samples collected from infants and children having acute diarrhea, the total number of E. Coli strains isolated were 64. Out of these 64 strains, 6 strains were found to be positive for Labile Toxin (LT) production by both latex agglutination and staphylococcal co-agglutination test. However, another 3 strains which were negative by latex agglutination were found to be positive by staph co-agglutination test. The latex agglutination test was found to be simple, easy and rapid. However this test was less sensitive in detection of ETEC. The staph-co-agglutination was also found to be simple, easy but it takes longer time (14 days). It was concluded that both tests are simple, inexpensive to perform and can be used for rapid detection of ETEC.
Ahmed
N
N
Microbiology and Immunology Department, Ain Shams University.
Kamal
A M
AM
el-Hamid
T A
TA
D000372:Agglutination Tests / Q000592:standards; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D003968:Diarrhea, Infantile / Q000382:microbiology; D004768:Enterotoxins; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D007841:Latex Fixation Tests / Q000592:standards; D012680:Sensitivity and Specificity
D004768:Enterotoxins