title: [In vitro granulopoietic cultures. Analysis using monoclonal antibodies].
abstract: Four monoclonal antibodies were applied, using an immunoenzymatic technique, the alkaline phosphatase anti-alkaline phosphatase, in granulopoietic cultures in vitro. In this manner, granulopoietic growth at tenth day of culture has been defined as positive for myeloid markers (CD15, CD11b) and negative for macrophagic (CD68) and proliferating cells (Ki 67) markers.
authors.affiliation: Servicio de Hematología, Hospital Clínico Universitario, Zaragoza.
authors.forename: F
authors.initials: F
authors.lastname: Puente
authors.forename: F
authors.initials: F
authors.lastname: Gómez Casal
authors.forename: C
authors.initials: C
authors.lastname: Vázquez
authors.forename: R
authors.initials: R
authors.lastname: Fuertes
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D008223:Lymphoma / Q000473:pathology; D008264:Macrophages; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015415:Biomarkers; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins
vernacular_title: Cultivos granulopoyéticos in vitro. Análisis con anticuerpos monoclonales.