DNA binding properties of c-Myc-related bHLH/LZ oncoproteins.
Structural features and DNA binding properties of bacterially expressed c-Myc related oncoproteins were examined. The circular dichroism spectra demonstrated highly alpha-helical nature of these proteins. Dimerization and the DNA binding of these proteins were examined by electrophoretic mobility shift assay.
Kurihara
Y
Y
Department of Bioengineering, Faculty of Engineering, Yokohama National University, Japan.
Horiuchi
M
M
Ukita
S
S
Katahira
M
M
Uesugi
S
S
D002942:Circular Dichroism; D018076:DNA, Complementary / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D016350:Leucine Zippers; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins