Calcium pump expression in human bone and soft tissue tumours.
Ninety-one cases of human bone and soft tissue tumours were studied for calcium pump expression by strepto-avidin-biotin immunohistochemical staining with a monoclonal antibody against sarcoplasmic reticulum calcium-ATPase (mAb6F5). Two out of 5 cases of embryonal rhabdomyosarcoma, 1 out of 5 cases of biphasic synovial sarcoma, 4 of 4 cases of chordoma and all of 3 chondrosarcoma cases were positive for mAb6F5. Although this novel monoclonal antibody can be used as a marker of myogenic tumours, the present positive result for endoplasmic reticulum calcium-ATPase (calcium pump) in other tumours including chordoma, chondrosarcoma and synovial sarcoma indicates a wider immunoreactivity. The findings further suggest that intracellular calcium may play an important role in cell proliferation and/or differentiation.
Kuroda
M
M
Department of Pathology, Faculty of Medicine, University of Tokyo, Japan.
Horiuchi
H
H
Oka
T
T
Ishida
T
T
Ono
A
A
Kawakita
M
M
Machinami
R
R
D000328:Adult; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002817:Chordoma / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012509:Sarcoma / Q000378:metabolism / Q000473:pathology; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D000252:Calcium-Transporting ATPases