title: Mutational analysis of the bacteriophage P2 Ogr protein: truncation of the carboxy terminus.
abstract: The Ogr protein is a 72-residue, zinc-binding transcription factor essential for activation of late gene expression in bacteriophage P2. Analysis of C-terminal truncated proteins generated by stop codon mutagenesis shows that deletion of residues distal to position 51 had negligible effects on Ogr function. More-extensive deletion resulted in unstable products with severely reduced activity. These results, as well as the effects of other mutations in this region, support the idea that the 21 C-terminal residues are not required for transactivation.
authors.affiliation: Institute of Biology, University of Oslo, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Gebhardt
authors.forename: R A
authors.initials: RA
authors.lastname: King
authors.forename: G E
authors.initials: GE
authors.lastname: Christie
authors.forename: B H
authors.initials: BH
authors.lastname: Lindqvist
mesh_terms: D000595:Amino Acid Sequence; D017901:Bacteriophage P2 / Q000235:genetics*; D004252:DNA Mutational Analysis; D015967:Gene Expression Regulation, Viral; D016153:Genes, Suppressor; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C074897:ogr protein, Enterobacteria phage P2