Purification of soluble murine interleukin 5 (IL-5) receptor alpha expressed in Chinese hamster ovary cells and its action as an IL-5 antagonist.
Recombinant soluble murine interleukin 5 receptor alpha (sIL-5R alpha) was expressed in Chinese hamster ovary cells and its biological activity was characterized. By using murine IL-5-coupled Sepharose, the single step purification method of the protein was established. The purified protein inhibited the murine IL-5-induced differentiation of BCL1 cells into immunoglobulin M-secreting cells in a dose-dependent manner. Moreover, it also inhibited murine IL-5-induced but not human IL-5-induced proliferation of TF-1 cells. These results indicated that sIL-5R alpha acted as an IL-5 antagonist and apparent species specificity of IL-5 action on murine cells was mediated, at least in part, by the alpha-chain of the IL-5 receptor.
Tsuruoka
N
N
Suntory Institute for Biomedical Research, Osaka, Japan.
Yamashiro
K
K
Tsujimoto
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015848:Interleukin-5 / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D007942:Leukemia, Experimental; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D015848:Interleukin-5; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D011994:Recombinant Proteins