Lymphokine gene transcription in CD4+CD8- T cells of a type III bare lymphocyte syndrome patient.
In this study we analyzed the impact of a MHC class-II-deficient environment on the differentiation of CD4+CD8- T lymphocytes into functional defined subsets of lymphokine-producing T-helper cells. To this end a CD4+CD8- T-cell line and CD4+CD8- T-cell clones, isolated from PBMCs of a type III BLS patient, were stimulated in vitro with anti-CD3 and PMA and assessed for lymphokine transcription patterns. The results of these analyses show that CD4+CD8- T cells that have matured in a MHC class-II-deficient environment display lymphokine transcription patterns that resemble those of MHC class-II-expressing family control-derived CD4+CD8- T cells.
Lambert
M
M
Department of Immunohematology and Blood Bank, Leiden University Hospital, The Netherlands.
van Eggermond
M
M
van den Elsen
P
P
D015704:CD4 Antigens / Q000032:analysis*; D016827:CD8 Antigens / Q000032:analysis*; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000502:physiology; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000378:metabolism*; D008222:Lymphokines / Q000235:genetics*; D016176:T-Lymphocyte Subsets / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D014158:Transcription, Genetic
D015704:CD4 Antigens; D016827:CD8 Antigens; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines