[In vitro granulopoietic cultures. Analysis using monoclonal antibodies].
Four monoclonal antibodies were applied, using an immunoenzymatic technique, the alkaline phosphatase anti-alkaline phosphatase, in granulopoietic cultures in vitro. In this manner, granulopoietic growth at tenth day of culture has been defined as positive for myeloid markers (CD15, CD11b) and negative for macrophagic (CD68) and proliferating cells (Ki 67) markers.
Puente
F
F
Servicio de Hematología, Hospital Clínico Universitario, Zaragoza.
Gómez Casal
F
F
Vázquez
C
C
Fuertes
R
R
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D008223:Lymphoma / Q000473:pathology; D008264:Macrophages; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015415:Biomarkers; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins
Cultivos granulopoyéticos in vitro. Análisis con anticuerpos monoclonales.