title: Brn-3.0: a POU-domain protein expressed in the sensory, immune, and endocrine systems that functions on elements distinct from known octamer motifs.
abstract: Characterization of Brn-3.0 and identification of a highly related member (Brn-3.1) of the class IV POU-domain family suggest potential roles of Brn-3.0 in the development of retinal ganglion cells and sensory neurons, as well as potential roles in the pituitary gland and the immune system. Brn-3.0 is expressed in the pituitary gland and in a corticotroph cell line. A functional DNA response element has been identified in the proopiomelanocortin promoter. In contrast to previously described mammalian POU-domain proteins, Brn-3.0 binds relatively ineffectively to known octamer DNA motifs, but instead binds with high affinity to a distinct set of DNA elements, functioning as a transcriptional activator. Brn-3.0, Brn-3.1, and the Drosophila tI-POU share an N-terminal region of homology, referred to as the "POU-IV box," which is similar to a conserved functional domain in the c-myc gene family.
authors.affiliation: Eukaryotic Regulatory Biology Program, Howard Hughes Medical Institute, University of California, San Diego.
authors.forename: M R
authors.initials: MR
authors.lastname: Gerrero
authors.forename: R J
authors.initials: RJ
authors.lastname: McEvilly
authors.forename: E
authors.initials: E
authors.lastname: Turner
authors.forename: C R
authors.initials: CR
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: O'Connell
authors.forename: K J
authors.initials: KJ
authors.lastname: Jenne
authors.forename: M V
authors.initials: MV
authors.lastname: Hobbs
authors.forename: M G
authors.initials: MG
authors.lastname: Rosenfeld
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004702:Endocrine Glands / Q000196:embryology / Q000378:metabolism; D015870:Gene Expression; D005796:Genes; D008221:Lymphoid Tissue / Q000196:embryology / Q000378:metabolism; D051379:Mice / Q000196:embryology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C494188:POU4F1 protein, human; C494189:Pou4f1 protein, mouse; D012333:RNA, Messenger; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors