Identification of the heat shock protein of Neurospora crassa corresponding to the stress-inducible peroxidase.
Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crassa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a approximately 10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.
Machwe
A
A
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Kapoor
M
M
D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis / Q000378:metabolism; D015870:Gene Expression; D005800:Genes, Fungal; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature; D008715:Methionine / Q000378:metabolism; D009492:Neurospora crassa / Q000378:metabolism*; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000032:analysis / Q000096:biosynthesis
D004271:DNA, Fungal; D006360:Heat-Shock Proteins; D012331:RNA, Fungal; D008715:Methionine; D010544:Peroxidases