Mapping of ben genes of Pseudomonas aeruginosa.
Four ben genes responsible for the conversion of benzoate to catechol in Pseudomonas aeruginosa PAO have been mapped to a 4.6 kb KpnI fragment, ben-1 and ben-4 were known to be separate genes but now ben-1508 has been found to be different from ben-2. The two genes were distinguished by Tn5 mutagenesis of a cosmid clone and deletion mapping. It is likely that the four genes mapped (ben-4, ben-2, ben-1508 and ben-1) correspond to the previously characterized benR (regulatory gene) and benABC (benzoate dioxygenase) respectively.
Zhang
C
C
Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.
Huang
M
M
Holloway
B W
BW
D001565:Benzoates / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D002874:Chromosome Mapping; D005798:Genes, Bacterial; D016254:Mutagenesis, Insertional; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015183:Restriction Mapping; D017384:Sequence Deletion
D001565:Benzoates; D002396:Catechols; C034221:catechol