title: The regulatory effect of substances released from porcine bronchial epithelial cells on hypoxic pulmonary vasoconstriction.
abstract: In this study, porcine pulmonary artery segments (intact or denuded) and bronchial segments (with or without epithelium) were tested in organ bath. Hypoxia caused a bronchial epithelium dependent relaxation of intact or denuded pulmonary artery preconstricted with phenylephrine. Constriction in intact pulmonary artery coated with epithelium denuded bronchus was observed during hypoxia. The bronchial epithelium-dependent relaxation could be inhibited by indomethacin but not be blocked by the following agents: atropine, propranolol, gossypol, methylene blue and chlorpheniramine. The results suggested that HPV was endothelium-dependent. Hypoxia could cause the production of an epithelium-derived relaxing factor (EpDRF), which acted directly on smooth muscle. The effect of EpDRF was not mediated by pulmonary endothelium, but might be related to arachidonic acid cyclooxygenase pathway.
authors.affiliation: Department of Pathophysiology, Tongji Medical University, Wuhan.
authors.forename: W H
authors.initials: WH
authors.lastname: Wang
authors.forename: D X
authors.initials: DX
authors.lastname: Wang
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000378:metabolism / Q000502:physiology*; D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D004848:Epithelium / Q000378:metabolism; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D011651:Pulmonary Artery / Q000502:physiology*; D013552:Swine; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D001685:Biological Factors; C056250:epithelium-derived relaxant factor; D007213:Indomethacin