Two new rat alpha-globin sequences as identified by the conserved region PCR.
Based on the notion that regions of structural genes which encode critical domains of the corresponding proteins are highly conserved among closely related species, oligonucleotide primers were designed and used to amplify the alpha-globin sequence(s) of the Sprague-Dawley (SD) rat. Data of these amplified sequence constructs showed that two new rat alpha-globin specific sequences have been identified. Southern hybridization confirmed the presence of these sequences in the rat genome.
Lam
V M
VM
Department of Biochemistry, University of Hong Kong.
Gu
Y L
YL
Au
D M
DM
Wong
W M
WM
Ma
C W
CW
Cheng
L Y
LY
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D005796:Genes; D005914:Globins / Q000235:genetics*; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats / Q000097:blood / Q000235:genetics*; D017208:Rats, Wistar / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D005914:Globins