Purification of yersiniabactin: a siderophore and possible virulence factor of Yersinia enterocolitica.
HPLC analysis revealed that Yersinia enterocolitica WA-C produced two substances under iron-limiting conditions one of which was identified as 2,3-dihydroxybenzoyl-L-serine. The other compound had iron-complexing activity and was called yersiniabactin. The fur mutant H1852 was shown to produce yersiniabactin constitutively in an iron-independent manner. Yersiniabactin was isolated by ethyl acetate extraction from the spent medium of H1852, size-fractionation chromatography and preparative HPLC. A catechol function was demonstrated with different chemical assays and by UV-visible spectroscopy. The molecular mass of yersiniabactin was determined to be 482 Da. Purified yersiniabactin stimulated growth of Y. enterocolitica and Escherichia coli phi under iron-limiting conditions and apparently served as an iron carrier. Transport of 55Fe-yersiniabactin was TonB-dependent, indicating a receptor-mediated uptake across the outer membrane. A pesticin-resistant mutant missing the receptor protein FyuA was unable to transport and use yersiniabactin as a siderophore.
Haag
H
H
Lehrstuhl für Mikrobiologie I, Universität Tübingen, Germany.
Hantke
K
K
Drechsel
H
H
Stojiljkovic
I
I
Jung
G
G
Zähner
H
H
D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D001430:Bacteriocins / Q000302:isolation & purification / Q000378:metabolism; D001693:Biological Transport, Active; D005285:Fermentation; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D017262:Siderophores / Q000302:isolation & purification* / Q000378:metabolism; D014774:Virulence; D015008:Yersinia enterocolitica / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity
D001425:Bacterial Outer Membrane Proteins; D001430:Bacteriocins; D007502:Iron Chelating Agents; D017262:Siderophores; D007501:Iron