Non-oscillatory free Ca2+ response of single B cells and WEHI-231 cells after cross-linking of antigen receptors with anti-immunoglobulin.
Cross-linking of surface-immunoglobulin (sIg) has been associated with IP3 production and a rise in cytoplasmic-free calcium ([Ca2+]i) in studies of populations of normal and transformed B cells. We have examined the kinetics of the induced cytoplasmic calcium rises in single, Fura-2-loaded cells, during stimulation with a variety of agonists. Our data indicate that the responses of B cells to some stimuli, such as elevated cyclic AMP, consist of repetitive calcium transients, but these Ca2+ oscillations do not occur after sIg ligation. Rather, sIg cross-linking leads to a rapid rise in cytoplasmic calcium which is followed by a sustained elevation. Most of this response seems to result from an inflow of extracellular calcium rather than from internal stores.
Greenwood
M R
MR
Department of Human Anatomy and Cell Biology, University of Liverpool, U.K.
Tasker
L
L
Cobbold
P H
PH
Marshall-Clarke
S
S
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003432:Cross-Linking Reagents; D000242:Cyclic AMP / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D011946:Receptors, Antigen / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D007136:Immunoglobulins; D011946:Receptors, Antigen; D000242:Cyclic AMP; D002118:Calcium