title: Plasmid encoded multi-drug resistance in Salmonella typhi from Pakistan.
abstract: Twenty-five isolates of Salmonella typhi made from cases of typhoid fever during 1990-1991 in Rawalpindi and Islamabad, were examined. All isolates were resistant to chloramphenicol, ampicillin, tetracycline, streptomycin, sulphamethoxazole and trimethoprim, but were sensitive to nalidixic acid, ofloxacin, ciprofloxacin, cefuroxime and cefotaxime. A single large 98 MDa plasmid was identified in all the isolates. The plasmid was self-transferable and encoded resistance to chloramphenicol, ampicillin, tetracycline, streptomycin, sulphamethoxazole and trimethoprim. Restriction endonuclease analysis patterns after EcoRI and HindIII digestion of the 98 MDa multi-drug resistance plasmids from each of the S. typhi isolates and transconjugants were the same.
authors.affiliation: Armed Forces Institute of Pathology, Rawalpindi, Pakistan.
authors.forename: S H
authors.initials: SH
authors.lastname: Mirza
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
mesh_terms: D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D010154:Pakistan; D010957:Plasmids / Q000235:genetics; D012485:Salmonella typhi / Q000187:drug effects* / Q000235:genetics; D014169:Transformation, Bacterial / Q000235:genetics*