Prevalence of human papillomaviruses 16 and 18 in transitional cell carcinoma of bladder.
It has been speculated that human papillomaviruses (HPV) might be a causative agent of transitional cell carcinoma of the bladder. With the polymerase chain reaction technic, fresh specimens of 53 transitional cell carcinomas of the bladder were examined, and bladder epithelium from 12 patients with hyperplasia of prostate and peripheral blood lymphocytes of 8 normal individuals served as controls. The primers set for HPV-16 and 18 were selected from E6/E7 regions of open reading frames. HPV-16 was positive in 52.8% of the specimens examined, whereas HPV-18 positive in only 3.7%. No controls showed either HPV-16 or 18. Reports on the role of HPVs in carcinogenesis of bladder are scanty and yet there exist conspicuous discrepancies among them. There is a diversity of opinions on the contamination and variation of technical procedures. More work should be done before the relationship between HPV-16 and development of bladder carcinoma can be clarified.
Yu
S T
ST
Department of Urology, Tianjin Medical College Hospital.
Wu
M M
MM
Li
L M
LM
D001483:Base Sequence; D002295:Carcinoma, Transitional Cell / Q000382:microbiology* / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000382:microbiology* / Q000473:pathology
D004279:DNA, Viral