Correlation between electroconvulsive seizure and HSC70 mRNA induction in mice brain.
The effects of electroconvulsive seizure and anti-convulsant drugs on induction of mRNA of heat shock protein were studied in mouse brain. Electrical shock induced mRNA of heat shock cognate protein (HSC70), but not heat shock protein (HSP70) mRNA. The induction was maximum 1 h after the ECS and continued for several hours, followed by long-lasting depression. Diazepam slightly prevented the ECS, but strongly attenuated the induction of HSC70 mRNA. Whereas phenytoin, which blocked the seizure, did not decrease but delayed the induction of HSC70 mRNA. The present results suggest that HSC70 mRNA level is increased with the ECS and that the induction level did not necessarily correlate the severity of the seizure.
Kaneko
M
M
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Abe
K
K
Kogure
K
K
Saito
H
H
Matsuki
N
N
D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D003975:Diazepam / Q000494:pharmacology / Q000627:therapeutic use; D004597:Electroshock / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005849:Gerbillinae; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D010672:Phenytoin / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D012640:Seizures / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control
D002352:Carrier Proteins; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C494372:Hspa8 protein, mouse; D012333:RNA, Messenger; D010672:Phenytoin; D003975:Diazepam