P53 protein expression in Hodgkin's disease.
P53 is an oncosuppressor gene which is located on chromosome 17. Mutations of the p53 gene are closely associated with malignant transformation under in vitro conditions and are the most common genetic alteration in human malignancy. Unlike normal p53 protein which is unstable and usually cannot be detected by immunohistology, mutated p53 shows a decreased cell turnover rate and overexpression as compared with the wild-type protein. In this study a panel of four anti-p53 antibodies (PAb240, PAb421, PAb1801 and DO7) was applied to 52 cases of Hodgkin's disease: three cases of nodular lymphocytic predominance (LP), 33 cases of nodular sclerosis (NS), and 16 cases of mixed cellularity (MC). The results show that 53 protein is present in the Hodgkin's- and Reed-Sternberg cells in 82% of NS and 94% of MC, but not in nodular LP. It is suggested that mutations of the p53 gene and loss of normal p53 function are frequent in Hodgkin's disease and may be implicated in the pathogenesis of this disease.
Lauritzen
A F
AF
Department of Pathology, Herlev Hospital, Denmark.
Hou-Jensen
K
K
Ralfkiaer
E
E
D000911:Antibodies, Monoclonal; D016158:Genes, p53; D006689:Hodgkin Disease / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D016159:Tumor Suppressor Protein p53