title: Determination of immunoglobulin classes and subclasses of sperm immobilizing antibodies in infertile women and the inhibitory effect of IgG4 subclass on the sperm immobilizing antibody activity.
abstract: Immunoglobulin classes and subclasses of sperm immobilizing antibodies (SI-Abs) in the sera of sterile women were determined by the absorption of patients' sera with Staphylococcus aureus and immunoadsorbents bound with class or subclass-specific anti-human immunoglobulin antibodies. Among 18 patients' sera tested, 16 had the IgG-dominant SI-Abs and the remaining 2 sera contained IgM-dominant SI-Abs. From the former patients' sera, 9 were further studied to determine the IgG subclasses; 6 of them had IgG1-dominant SI-Abs, one IgG2-dominant, and in the other two IgG1 and IgG2 were equally dominant. Interestingly, SI-Ab activities in 6 of the 9 patients' sera increased after absorption of IgG4 subclass and the addition of IgG4 purified from an SI-positive patient to the same patient's serum diminished the SI-Ab activities.
authors.affiliation: Department of Obstetrics and Gynecology, Hyogo Medical College, Nishinomiya, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuji
authors.forename: T
authors.initials: T
authors.lastname: Tatsumi
authors.forename: K
authors.initials: K
authors.lastname: Kameda
authors.forename: K
authors.initials: K
authors.lastname: Koyama
authors.forename: S
authors.initials: S
authors.lastname: Isojima
mesh_terms: D003167:Complement Activation; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification / Q000502:physiology*; D007132:Immunoglobulin Isotypes / Q000097:blood*; D007247:Infertility, Female / Q000276:immunology*; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes