Cell-associated hemagglutinin of classical vibrio cholerae O1 with reference to intestinal adhesion.
Vibrio cholerae O1 86B3 biovar cholerae has at least two types of cell-associated hemagglutinin. One is cell wall-associated and L-fucose sensitive, whereas the other is pili-associated and D-mannose sensitive. A pilus rich variant of 86B3 and a poorly piliated parent strain adhered equally to the rabbit intestine. This adhesion was inhibited by L-fucose but not by D-mannose. A Fab fraction prepared from anti-pilus antibody did not inhibit the adhesion. These results suggest that not the pili but a colonization factor located in the outer membrane of the organisms plays a role in intestinal adhesion.
Nakasone
N
N
Department of Bacteriology, University of the Ryukyus School of Medicine, Okinawa, Japan.
Iwanaga
M
M
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D006388:Hemagglutinins / Q000502:physiology*; D007422:Intestines / Q000382:microbiology*; D011817:Rabbits; D011971:Receptors, Immunologic / Q000502:physiology*; D014734:Vibrio cholerae / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure
D006388:Hemagglutinins; D011971:Receptors, Immunologic; C037872:pili, bacterial receptor