title: Modulation of tensin and vimentin expression in chick embryo developing cartilage and cultured differentiating chondrocytes.
abstract: It has been proposed that tensin, in association with several other proteins, mediates the micro-filament-integrin link. Here we describe the isolation of clones spanning about 5 kb from the 3' end of tensin mRNA from cultured chick embryo chondrocyte and embryonic heart cDNA libraries. Tensin expression was investigated in cultured chick embryo cells. It was observed that tensin expression is dependent upon substrate adhesion and it is turned off after 7 days of suspension culture. This process is reversible. Tensin expression is also regulated during cartilage cell differentiation in vivo; at Hamburger and Hamilton stage 39-40, non-hypertrophic tibial chondrocytes express both RNA and protein while hypertrophic chondrocytes do not. In the culture system the expression of vimentin, a major component of intermediate filaments, showed an opposite behaviour since the suspension culture enhances the accumulation of both vimentin and its mRNAs. Therefore in chick embryo cultured chondrocytes and in vivo, during cartilage development, cell shape changes and/or integrin-extracellular matrix protein interactions may be involved in the regulation of these two genes coding for cytoskeletal proteins.
authors.affiliation: Laboratorio di Differenziamento Cellulare, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
authors.forename: R
authors.initials: R
authors.lastname: van de Werken
authors.forename: M
authors.initials: M
authors.lastname: Gennari
authors.forename: S
authors.initials: S
authors.lastname: Tavella
authors.forename: P
authors.initials: P
authors.lastname: Bet
authors.forename: F
authors.initials: F
authors.lastname: Molina
authors.forename: S
authors.initials: S
authors.lastname: Lin
authors.forename: R
authors.initials: R
authors.lastname: Cancedda
authors.forename: P
authors.initials: P
authors.lastname: Castagnola
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002356:Cartilage / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D015536:Down-Regulation; D017403:In Situ Hybridization; D008840:Microfilament Proteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry; D000070876:Tensins; D014746:Vimentin / Q000096:biosynthesis*
chemical_list: D018076:DNA, Complementary; D008840:Microfilament Proteins; D012333:RNA, Messenger; D000070876:Tensins; D014746:Vimentin