The production of polyclonal antibodies against the mycotoxin derivative patulin hemiglutarate.
The mycotoxin patulin is a toxic, carcinogenic, unsaturated lactone produced by a number of molds. Polyclonal antibodies against patulin hemiglutarate were produced. Specific antibodies against patulin alone, however, were not clearly demonstrated. Because of its low molecular weight, patulin required conjugation to bovine serum albumin (BSA) to increase its immunogenicity. Anti-patulin-hemiglutarate-BSA antibody titer and specificity were determined using indirect and indirect competitive ELISA, respectively. Immunoassays would facilitate detection and quantitation of patulin.
McElroy
L J
LJ
Department of Microbiology, Pennsylvania State University, Monaca 15061.
Weiss
C M
CM
D000818:Animals; D000917:Antibody Formation; D002273:Carcinogens; D004797:Enzyme-Linked Immunosorbent Assay; D009153:Mutagens; D010365:Patulin / Q000031:analogs & derivatives* / Q000276:immunology; D011817:Rabbits
D002273:Carcinogens; D009153:Mutagens; C084421:patulin hemiglutarate; D010365:Patulin