title: Expression of cytokine receptors in cultured neuronal and glial cells.
abstract: We investigated mRNA expression of cytokine receptors in three different types of glial cells and two neuronal line cells by the RT-PCR method. Microglia expressed mRNA for receptors of IL-3, -4, -6, -7, GM-CSF, and M-CSF. Astrocytes were positive for receptors of IL-6, -7, GM-CSF, and M-CSF. Oligodendrocytes were positive for receptors of IL-3, -4, -7, GM-CSF, and M-CSF. Neuronal cells expressed receptors of IL-6 and GM-CSF with very low levels. This is the first demonstration of cytokine receptor mRNA expression in isolated glial and neuronal cells.
authors.affiliation: Division of Cell Biology, Fujita Health University, Aichi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sawada
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: A
authors.initials: A
authors.lastname: Suzumura
authors.forename: T
authors.initials: T
authors.lastname: Marunouchi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism; D002478:Cells, Cultured; D051379:Mice; D008809:Mice, Inbred C3H; D017628:Microglia / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D018123:Receptors, Interleukin / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D018123:Receptors, Interleukin; D016186:Receptor, Macrophage Colony-Stimulating Factor