Importance of specific purine-pyrimidine amino and hydroxyl groups for efficient cleavage by a hammerhead ribozyme.
Subsequent of the discovery that RNA can have site specific cleavage activity, there has been a great deal of interest in the design and testing of trans-acting catalytic RNAs as therapeutic agents. We have chemically synthesized and experimentally analyzed chimeric DNA-RNA ribozymes in which the catalytic center remains as RNA.
Tanaka
H
H
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Hosaka
H
H
Takahashi
R
R
Imamura
Y
Y
Takai
K
K
Yokoyama
S
S
Takaku
H
H
D001483:Base Sequence; D002384:Catalysis; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011687:Purines / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*
D011687:Purines; D011743:Pyrimidines; D016337:RNA, Catalytic; D017665:Hydroxyl Radical