ATP and UTP increase secretion of bronchial inhibitor by human tracheal gland cells in culture.
The effects of ATP and UTP on intracellular Ca2+ levels and on the secretion of the bronchial inhibitor and high-molecular-weight glycoproteins were studied in cultures of human bronchotracheal gland cells. ATP, adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S), and UTP increased intracellular Ca2+ levels in a manner that was partially dependent on the presence of extracellular Ca2+. Other nucleotides (ADP, alpha,beta-methylene ATP, beta,gamma-methylene ATP, and 2-methylthio ATP) and adenosine were ineffective, thus suggesting the presence of a "nucleotide" receptor specific for ATP and UTP. At concentrations similar to those that raised intracellular Ca2+ concentration, ATP, UTP, and ATP gamma S stimulate the secretion of the bronchial inhibitor. ATP and UTP also increase the production of sulfated high-molecular-weight glycoproteins. These results indicate the presence in human tracheal gland cells of a nucleotide receptor that mediates intracellular Ca2+ mobilization and controls the secretion of macromolecules.
Merten
M D
MD
Groupe de Recherche sur les Glandes Exocrines 27, Marseille, France.
Breittmayer
J P
JP
Figarella
C
C
Frelin
C
C
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000328:Adult; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D009092:Mucous Membrane / Q000187:drug effects / Q000378:metabolism; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D013997:Time Factors; D014132:Trachea / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000494:pharmacology*
D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D002217:Carbachol; D002118:Calcium; D014544:Uridine Triphosphate