Transformation of Trichoderma reesei with the Hormoconis resinae glucoamylase P (gamP) gene: production of a heterologous glucoamylase by Trichoderma reesei.
A cDNA encoding for the glucoamylase P enzyme (GAMP) of the fungus Hormoconis resinae was introduced into the cellulolytic filamentous fungus Trichoderma reesei under the control of the promoter of the major cellulase gene (cbh1) of Trichoderma. The transforming vector plasmid used was found to be integrated into the genome of T. reesei at various locations and in multiple copies. The size of the GAMP secreted by Trichoderma varied because of different glycosylation patterns. The best transformant strains secreted about 700 mg/l of active GAMP, which is 20-fold more than obtained with H. resinae.
Joutsjoki
V V
VV
Research Laboratories, Alko Ltd., Helsinki, Finland.
Torkkeli
T K
TK
Nevalainen
K M
KM
D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000235:genetics*; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013728:Terminator Regions, Genetic; D014170:Transformation, Genetic; D014242:Trichoderma / Q000235:genetics*
D005087:Glucan 1,4-alpha-Glucosidase