Lack of effect of ammonium glycyrrhizinate on the morphology of ovine nasal mucosa in vitro.
Glycyrrhetinic acid derivatives are reported to be nasal absorption promoters (1). Effects of ammonium glycyrrhizinate (AMGZ) on the in vitro morphology of ovine nasal mucosa were therefore examined by light and electron microscopy. Nasal mucosa was stripped from the submucosa and mounted in Ussing chambers. Exposure of the apical surface to 2% ammonium glycyrrhizinate (24 mM) for 90 min caused no histopathological changes to the nasal epithelium. Epithelial integrity remained intact as evidenced by the continued presence of morphologically intact junctional complexes. No sloughing of the epithelial layer from the basement membrane was observed, and cilia and microvilli were not affected by treatment with AMGZ. The results indicate that short-term exposure in vitro to ammonium glycyrrhizinate caused no overt morphological damage to ovine nasal mucosa.
Reardon
P M
PM
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence.
Wall
D A
DA
Hart
T K
TK
Smith
P L
PL
Gochoco
C H
CH
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002923:Cilia / Q000187:drug effects / Q000648:ultrastructure; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D019695:Glycyrrhizic Acid; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D009297:Nasal Mucosa / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D010539:Permeability; D012756:Sheep
D000935:Antifungal Agents; D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid