Genetic and enzymatic basis for the differential expression of GM2 and GD2 gangliosides in human cancer cell lines.
Using beta 1,4-N-acetylgalactosaminyltransferase (EC 2.4.1.92) complementary DNA, the correlation of gene expression, enzyme activity, and expression of ganglioside antigens was analyzed in 20 human tumor cell lines. In many lines, GM2 and/or GD2 were the most complex structures examined. Northern blot analysis revealed 5.2- and 3.0-kilobase mRNAs in almost all cell lines expressing GD2 and/or GM2. Some melanoma lines, however, showed no bands although they expressed fairly high levels of GD2. These cell lines expressed very high levels of alpha 2,8-sialyltransferase and the resulting product, GD3. Semiquantitative RT-PCR demonstrated that even cell lines with no bands in Northern blot contained 0.4-2.5% of mRNA level in the highest expressing cell line. These results indicate that GD2 expression on individual cell lines is regulated not only by the expression level of the N-acetylgalactosaminyl transferase but also by the amount of its precursor structure (GD3) and alpha 2,8-sialyltransferase present in the cells. beta 1,4-N-acetylgalactosaminyltransferase activities and mRNA levels generally correlated quite closely. A few lines, however, showed lower enzyme activities than expected from their mRNA levels, indicating the possibility that the enzyme is being regulated by translational or posttranslational modification such as phosphorylation and glycosylation as well as by transcriptional regulation. Depending on their patterns of ganglioside synthesis and expression, the lines examined were classified into 6 groups which were characteristic of different tumor cell types.
Yamashiro
S
S
Department of Oncology, Nagasaki University School of Medicine, Japan.
Ruan
S
S
Furukawa
K
K
Tai
T
T
Lloyd
K O
KO
Shiku
H
H
Furukawa
K
K
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015139:Blotting, Southern; D005678:G(M2) Ganglioside / Q000032:analysis* / Q000378:metabolism; D005679:G(M3) Ganglioside / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism; D005732:Gangliosides / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D005679:G(M3) Ganglioside; D005732:Gangliosides; D012333:RNA, Messenger; D005678:G(M2) Ganglioside; C019403:ganglioside, GD2; D005700:Galactosyltransferases; D017350:N-Acetylgalactosaminyltransferases