title: Generation and partial characterization of an eosinophil chemotactic cytokine produced by sensitized equine mononuclear cells stimulated with Strongylus vulgaris antigen.
abstract: Supernatants generated by stimulation of peripheral blood mononuclear cells (PBMC) from Strongylus vulgaris sensitized or immunized ponies were assayed in vitro for eosinophil chemotactic activity (ECA) using the filter system in blind well chambers. The supernatants from these cultures were chemotactic for eosinophils, but not for neutrophils. Supernates from cultures of unsensitized PBMC stimulated with S. vulgaris antigen were not chemotactic for eosinophils. ECA was first detected in culture supernatants after 1.5 h of incubation and was dependent on both antigen and PBMC concentrations, but independent of serum concentrations. Both female and male S. vulgaris worm antigens stimulated ECA production from sensitized PBMC. ECA was not induced by in vitro stimulation of sensitized S. vulgaris PBMC by female Strongylus edentatus worm antigen. Partial characterization of the eosinophil chemotactic cytokine showed it to be nondialyzable, greater than 8000 molecular weight (MW), and sensitive to heating (56 and 95 degrees C), trypsin, and sodium metaperiodate treatments, suggesting that the cytokine is a protein containing some essential carbohydrate moieties. The cytokine described in this paper could partially contribute to the in vivo blood and tissue eosinophilia in experimental S. vulgaris infection.
authors.affiliation: Department of Veterinary Science, Louisiana State University Agricultural Center, Baton Rouge 70803.
authors.forename: V A
authors.initials: VA
authors.lastname: Dennis
authors.forename: T R
authors.initials: TR
authors.lastname: Klei
authors.forename: M R
authors.initials: MR
authors.lastname: Chapman
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D002478:Cells, Cultured; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis* / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006736:Horses / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000276:immunology; D013323:Strongylus / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D002631:Chemotactic Factors, Eosinophil; D016207:Cytokines