Neisseria meningitidis: serotyping and subtyping by whole cell ELISA.
Twenty strains of Neisseria meningitidis isolated from clinically diagnosed cases of meningococcal disease were subjected to serogrouping, employing slide agglutination followed by serotyping and serosubtyping by whole cell ELISA using monoclonal typing antisera. All isolates were from sporadic cases of meningitis during a period of two years from various hospitals in Delhi. All 20 isolates were grouped as serogroup A and typed as serotype 4, except one strain which was untypable. On serosubtyping the isolates were found to belong to P1.9 (7 strains) followed by P1.1 (5), P1.9 (2), P1.16,1 (2), P1.6,10 (2), P1.10,7,1 (1) and non-subtypable (1).
Prakash
K
K
Department of Microbiology, Lady Hardinge Medical College, New Delhi, India.
Lakshmy
A
A
Malhotra
V L
VL
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009345:Neisseria meningitidis / Q000145:classification*; D012703:Serotyping / Q000379:methods*