title: A novel lectin-based enzyme-linked immunosorbent assay for the measurement of IgA1 in serum and secretory IgA1 in secretions.
abstract: Jackfruit lectin (Jacalin) specifically binds IgA1 and secretory IgA1 (sIgA1). Jacalin was successfully purified, and used as the immobilized phase in novel noncompetitive enzyme-linked immunosorbent assays (ELISAs) for the measurement of IgA1 in serum and sIgA1 in saliva, using jacalin affinity purified material as standards. A non-competitive ELISA for the measurement of total sIgA was also developed using commercially available reagents.
authors.affiliation: Immunology Department, University College Hospital, Galway, Ireland.
authors.forename: M J
authors.initials: MJ
authors.lastname: Cullina
authors.forename: J F
authors.initials: JF
authors.lastname: Greally
mesh_terms: D002846:Chromatography, Affinity; D003126:Colostrum / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007202:Indicators and Reagents; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification; D037121:Plant Lectins; D011506:Proteins / Q000032:analysis; D012463:Saliva / Q000737:chemistry
chemical_list: D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007202:Indicators and Reagents; D037102:Lectins; D037121:Plant Lectins; D011506:Proteins; C043696:jacalin