Low-dose heparin inhibits acute graft versus host disease in mice.
We have studied the effect of low-dose heparin on GVHD, marrow engraftment and graft-versus-leukemia (GVL) effects in an experimental murine model. Recipient (C57BL/6 x BALB/c) F1 mice were transplanted with C57BL/6 marrow and/or spleen cells and treated with daily sc injection of 5 micrograms heparin for 30 days. We have shown that heparin in low doses attenuates the severity of acute GVHD and reduces the mortality rate from 69 to 37.5% without abrogating the GVL effect induced by the allograft and without impairing marrow engraftment.
Naparstek
E
E
Department of Bone Marrow Transplantation, Hadassah University Hospital, Jerusalem, Israel.
Slavin
S
S
Weiss
L
L
Sidi
H
H
Ohana
M
M
Reich
S
S
Vlodavsky
I
I
Cohen
I R
IR
Naparstek
Y
Y
D000208:Acute Disease; D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects* / Q000276:immunology; D006085:Graft Survival / Q000187:drug effects; D006086:Graft vs Host Disease / Q000517:prevention & control*; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D007279:Injections, Subcutaneous; D007942:Leukemia, Experimental / Q000276:immunology* / Q000601:surgery; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D011828:Radiation Chimera; D013154:Spleen / Q000637:transplantation; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D006493:Heparin