Regulation of the phosphorylation of histones and glycogen synthase.
The phosphorylation of histones and glycogen synthase by protein kinases was analysed by SDS-polyacrylamide gel electrophoresis and autoradiography. The phosphorylation of histone III-S by the catalytic subunit of cyclic AMP-dependent protein kinase (A-PK) or cGMP-dependent protein kinase (G-PK) was inhibited by archidonic acid, sphingosine and staurosporine. Using the catalytic subunit of A-PK, the phosphorylation of histone VIII-S was inhibited by Ca2+, arachidonic acid and staurosporine; the phosphorylation of histone II-S was inhibited by phosphatidyl ethanolamine, phosphatidyl inositol, arachidonic acid and staurosporine; and the phosphorylation of glycogen synthase was inhibited by arachidonic acid and staurosporine. After being phosphorylated by the catalytic subunit of A-PK, calpain II with 4 microM Ca2+ was less effective in degrading histone III-S, which had been prephosphorylated by PK-C.
Kuo
W N
WN
Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32115.
Ganesan
U
U
Davis
D L
DL
Walbey
D L
DL
Bell
M A
MA
Allen
K
K
Siddeeq
S
S
McCall
L K
LK
Carwell
N
N
D055372:AMP-Activated Protein Kinases; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001345:Autoradiography; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006006:Glycogen Synthase / Q000378:metabolism* / Q000502:physiology; D051528:Guanylate Kinases; D006657:Histones / Q000378:metabolism* / Q000502:physiology; D009097:Multienzyme Complexes / Q000494:pharmacology; D009703:Nucleoside-Phosphate Kinase / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000494:pharmacology; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases; D011817:Rabbits; D013110:Sphingosine / Q000494:pharmacology; D019311:Staurosporine; D013552:Swine
D000470:Alkaloids; D006657:Histones; D009097:Multienzyme Complexes; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D047428:Protein Kinase Inhibitors; D016718:Arachidonic Acid; D006006:Glycogen Synthase; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; D009703:Nucleoside-Phosphate Kinase; D051528:Guanylate Kinases; D019311:Staurosporine; D013110:Sphingosine