title: Functional analysis of mutated cDNA clones by direct use of PCR products in in vitro transcription/translation reactions.
authors.affiliation: Laboratorium für Molekulare Biologie, Genzentrum, Martinsried, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Henkel
authors.forename: P A
authors.initials: PA
authors.lastname: Baeuerle
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000378:metabolism; D004926:Escherichia coli; D006801:Humans; D020672:I-kappa B Proteins; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000096:biosynthesis* / Q000378:metabolism; D016376:Oligonucleotides, Antisense; D010766:Phosphorylation; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; D046911:Macromolecular Substances; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D016376:Oligonucleotides, Antisense; D000072000:NF-KappaB Inhibitor alpha