Heterogeneity in the 5' untranslated region of the rat glucocorticoid receptor mRNA.
We have cloned several novel sequences upstream from the first coding exon of the rat glucocorticoid receptor (GR) mRNA using PCR. Analysis of these sequences in RNase protection assays showed that one of the cloned sequences represents the major GR 5' non-coding exon which is expressed in all tissues studied, both at different stages of development and under different hormonal conditions. This major exon is homologous to the human GR 5' untranslated region (UTR). Three other sequences were cloned, but could not be detected in the RNase protection assay, suggesting that they are only minor transcripts, at least under the varying conditions of GR expression studied. One of these sequences is identical to a previously described rat GR cDNA sequence, while another was shown to be contiguous with the rat genomic DNA sequence.
Gearing
K L
KL
Karolinska Institute, Department of Medical Nutrition, Huddinge University Hospital, F60 NOVUM, Sweden.
Cairns
W
W
Okret
S
S
Gustafsson
J A
JA
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid