In situ hybridization: a simple and sensitive method for detection of trisomy 12 in chronic lymphocytic leukemia.
Chromosome aberrations are detected in only 50% of patients with chronic lymphocytic leukemia (CLL), owing usually to the low mitotic rate exhibited by the neoplastic lymphocytes. Fluorescence in situ hybridization (FISH) is a simple method for identifying numerical abnormalities of the target chromosome in interphase nuclei. Therefore, we used the FISH procedure with chromosome 12-specific a-satellite probe to evaluate 19 patients with CLL. Trisomy 12 was detected in interphase cells of 12 patients (63%). Cytogenetic analysis, performed in nine patients, yielded trisomy 12 in four (44%). FISH detected three patients with trisomy 12 in whom conventional cytogenetic method yielded a normal karyotype. FISH is a simple, reliable, and sensitive method for detection of trisomy 12 in patients with CLL.
Lalkin
A
A
Department of Medicine, Meir Hospital, Kfar Saba, Israel.
Lishner
M
M
Gaber
E
E
Manor
Y
Y
Fejgin
M
M
Ravid
M
M
Amiel
A
A
D000368:Aged; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014314:Trisomy / Q000175:diagnosis*