Production and properties of a factor X-cellulose-binding domain fusion protein.
A fusion protein, FX-CBDCex, which comprises factor X with a cellulose-binding domain (CBDCex) fused to its C-terminus, was produced in BHK cells. It was purified from the culture medium by affinity chromatography on cellulose. FX-CBDCex could be activated to FXa-CBDCex with Russell viper venom. FXa-CBDCex was as active as FXa against a chromogenic substrate and against proteins containing the Ile-Glu-Gly-Arg sequence hydrolysed by FXa. FXa-CBDCex retained its activity when adsorbed to cellulose.
Assouline
Z
Z
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Shen
H
H
Kilburn
D G
DG
Warren
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015153:Blotting, Western; D002460:Cell Line; D002482:Cellulose / Q000378:metabolism*; D002846:Chromatography, Affinity; D002863:Chromogenic Compounds / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D005170:Factor X / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007668:Kidney; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D014162:Transfection
D002863:Chromogenic Compounds; D011993:Recombinant Fusion Proteins; D005170:Factor X; D002482:Cellulose