Enhanced cardiac preservation with oxygenated University of Wisconsin solution.
The purpose of this study was to determine if oxygen delivery to rabbit cardiac allografts arrested and stored in University of Wisconsin solution (UWS) at 4 degrees C would affect preservation. Nineteen isolated rabbit hearts were rapidly excised and perfused at 80 mm Hg on an isovolumic modified Langendorff apparatus. A micromanometer was placed within a balloon and inserted into the left ventricle through the mitral valve orifice. Digitized pressure waveforms were collected at 11 known balloon volumes from 0.8 to 1.2 ml. Baseline data were obtained for all hearts while perfused with Krebs-Henseleit solution equilibrated with 95% O2:5% CO2 at 37 degrees C. All hearts were arrested with 30 ml of UWS (290 mOsm). The control group (N = 10) was stored in UWS at 4 degrees C for 8 hr, and the experimental group (N = 9) was perfused with oxygenated UWS (O2 content = 5.6 ml O2) at 4 degrees C for 8 hr at a pressure of 60 mm Hg (5-10 ml/min). Both groups were then reperfused with Krebs-Henseleit buffer at 80 mm Hg for 15 min at for postpreservation data acquisition. Left ventricular developed pressures over a physiologic range (pressure-volume area) and maximum positive and negative dP/dt were calculated. Recovery of left ventricular parameters as a percentage of the baseline values was determined. Mean pressure-volume area recovery in the nonperfused group was 40 +/- 7.9% versus the perfused group (71 +/- 7.0%, P = 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Peterseim
D S
DS
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
Meyers
C H
CH
Craig
D M
DM
Heinle
J S
JS
Jayawant
A M
AM
Devaney
J J
JJ
Klitzman
B M
BM
Van Trigt
P
P
D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D005978:Glutathione / Q000494:pharmacology; D006321:Heart / Q000187:drug effects*; D006324:Heart Arrest, Induced; D007328:Insulin / Q000494:pharmacology; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010100:Oxygen / Q000494:pharmacology*; D010477:Perfusion; D011312:Pressure; D011817:Rabbits; D011887:Raffinose / Q000494:pharmacology; D013599:Systole; D016277:Ventricular Function, Left / Q000187:drug effects
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen