title: Reversed-phase and ion-pair separations of nucleotides, nucleosides and nucleobases: analysis of biological samples in health and disease.
abstract: Methods for the assay of nucleotides, nucleosides and nucleobases in biological samples in health and disease are reviewed, with emphasis on reversed-phase and ion-pair reversed-phase techniques for their determination. Modes of extraction from biological samples are discussed with respect of the determination of in vivo concentrations. Advantages and limitations of ion-pair reversed-phase chromatography are discussed with examples from biochemistry and clinical chemistry. The capacity of the high-performance capillary electrophoresis is compared with that of ion-pair reversed-phase chromatography.
authors.affiliation: Sandoz Pharma Ltd., Basle, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Werner
mesh_terms: D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*
chemical_list: D009705:Nucleosides; D009711:Nucleotides