Estimation of the genetic variation in complement activity of common carp (Cyprinus carpio L.).
The complement status of hybrid, laboratory raised carp was determined by an in vitro approach of the alternate complement activity (ACH50) and total haemolytic activity (CH50), and by measurement of serum C3 levels. The lysis of target sheep red blood cells (RBC) in the haemolytic assay for CH50 activity depended, amongst others, on the haemolysin concentration in the assay. Rocket electrophoresis showed a mean serum C3 concentration of 0.95 mg ml-1. The variation for both ACH50 and CH50 haemolytic activity was approximately 30%. The degree of genetic determination of the parameters was investigated by estimation of their repeatabilities, which were relatively high for CH50 (0.71) and ACH50 activity (0.72), but lower for C3 levels (0.54). Correlations between ACH50 values and C3 levels were significant, but moderate (0.54-0.58).
Wiegertjes
G F
GF
Department of Experimental Animal Morphology and Cell Biology, Agricultural University, Wageningen, Netherlands.
Yano
T
T
van Muiswinkel
W B
WB
D000818:Animals; D002347:Carps / Q000276:immunology*; D015932:Complement C3c / Q000235:genetics*; D015941:Complement Hemolytic Activity Assay; D003170:Complement Pathway, Alternative / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D014644:Genetic Variation; D006460:Hemolysin Proteins / Q000276:immunology; D007122:Immunoelectrophoresis; D008297:Male
D006460:Hemolysin Proteins; D015932:Complement C3c