Localization of the gene (RSN) coding for restin, a marker for Reed-Sternberg cells in Hodgkin's disease, to human chromosome band 12q24.3 and YAC cloning of the locus.
A novel 160-kDa intermediate filament associated protein, named restin (Reed-Sternberg intermediate filament associated protein), is specifically expressed in the malignant cells of Hodgkin's disease and anaplastic large cell lymphoma (Ki-1 lymphoma). The combination of chromosomal R-banding and fluorescence in situ hybridization (FISH) with the use of two fluorescent dyes, fluorescein isothiocyanate and propidium iodide, allowed simultaneous detection of the hybridized DNA sequence and chromosomal R-banding. By this technique, the gene coding for restin (RSN) was assigned to chromosome region 12q24.31-->q24.33, while localization of the alpha-2-macroglobulin receptor (A2MR) was refined to 12q13.1-->q13.3. To further analyze the restin gene, a 500-kb YAC clone containing the gene was isolated and analyzed. A restriction map of this area is presented.
Hilliker
C
C
Center for Human Genetics, Katholieke Universiteit van Leuven, Belgium.
Delabie
J
J
Speleman
F
F
Bilbe
G
G
Bruggen
J
J
Van Leuven
F
F
Van den Berghe
H
H
D002871:Chromosome Banding; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins / Q000235:genetics*; D016539:Reed-Sternberg Cells / Q000648:ultrastructure*; D015183:Restriction Mapping
D018076:DNA, Complementary; D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; C076744:cytoplasmic linker protein 170