The micronucleus assay in mouse peripheral blood reticulocytes demonstrates the transmission of chromosomal instability induced by mitomycin C and benzo[a]pyrene.
The frequency of micronuclei (MN) in mouse peripheral blood reticulocytes supravitally stained with acridine orange was assessed at different time intervals after treatment in vivo with two dose levels of mitomycin C (MMC) and benzo[a]pyrene. Increased frequencies were observed for many days after treatment, indicating that MN may be produced as a consequence of chromosomal instability transmitted by proliferating erythroblasts. These results confirm previous evidence of the persistent cytogenetic effects of MMC and suggest that clastogenic agents acting by different primary lesions may have a similar ability to induce this effect.
Russo
A
A
Department of Biology, University of Padova, Italy.
Dorigo
E
E
Renzi
L
L
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D016009:Chi-Square Distribution; D004900:Erythroblasts / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests / Q000379:methods; D016685:Mitomycin / Q000633:toxicity*; D016296:Mutagenesis; D009153:Mutagens / Q000633:toxicity*; D012156:Reticulocytes / Q000187:drug effects*; D013997:Time Factors
D009153:Mutagens; D001564:Benzo(a)pyrene; D016685:Mitomycin