title: Differential effect of chronic ethanol treatment on barbiturate and steroid modulation of muscimol-binding to rat brain cortex.
abstract: We report the differential alterations produced by chronic ethanol treatment on the modulation, by the barbiturate thiopental and the steroid 5 beta-pregnan-3 alpha-ol-20-one, of the binding of [3H]muscimol to membrane preparations from rat brain cortex. We found a clear barbiturate- and steroid-promoted enhancement of muscimol-binding to membranes in both control and ethanol-treated animals. However, the enhancements were higher in control animals, using the barbiturate, and in ethanol-treated rats, using the steroid, Bmax and Kd values were also differentially affected in control and ethanol-treated animals by the presence of the barbiturate or the steroid.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Negro
authors.forename: E
authors.initials: E
authors.lastname: Casanova
authors.forename: M A
authors.initials: MA
authors.lastname: Chinchetru
authors.forename: A
authors.initials: A
authors.lastname: Fernández-López
authors.forename: P
authors.initials: P
authors.lastname: Calvo
mesh_terms: D000818:Animals; D001463:Barbiturates / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009118:Muscimol / Q000378:metabolism*; D011280:Pregnanolone / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology*; D013874:Thiopental / Q000494:pharmacology
chemical_list: D001463:Barbiturates; D011963:Receptors, GABA-A; D013256:Steroids; D009118:Muscimol; D000431:Ethanol; D011280:Pregnanolone; D013874:Thiopental