title: Influence of collagen network on left ventricular systolic and diastolic function in aortic valve disease.
abstract: OBJECTIVE
  The purpose of this study was to evaluate left ventricular structure-function interplay in aortic valve disease.
  
  
  BACKGROUND
  An increase in myocardial fibrosis has been demonstrated in aortic valve disease, but changes in the collagen network and their effect on ventricular function have not been defined.
  
  
  METHODS
  Left ventricular structure was assessed from left ventricular endomyocardial biopsy specimens obtained in 32 patients with aortic valve disease (aortic stenosis in 25, aortic regurgitation in 7). Total collagen volume fraction, orthogonal collagen fiber meshwork (cross-hatching), endocardial fibrosis, muscle fiber diameter and volume fraction of myofibrils were determined by morphologic-morphometric evaluation. Control biopsy data were obtained from six donor hearts before transplantation. Eleven other patients with normal left ventricular function served as hemodynamic status control subjects. Left ventricular biplane cineangiography and high fidelity pressure measurements were carried out in all patients. Systolic function was assessed from ejection fraction. Diastolic function was evaluated by the time constant of relaxation, early and late peak filling rates and the constant of passive myocardial stiffness. Patients were assigned to three groups according to increasing severity of nonmyocyte tissue alterations. Group 1 comprised 10 patients with elevated total collagen volume fraction. Group 2 comprised 6 patients with normal total collagen volume fraction and the presence of increased cross-hatching or endocardial fibrosis, or both. Group 3 comprised 16 patients with elevated total collagen volume fraction and the presence of cross-hatching or endocardial fibrosis, or both.
  
  
  RESULTS
  Muscle fiber diameter was increased in the three groups with aortic valve disease, whereas the volume fraction of myofibrils was comparable in all four study groups. Ejection fraction was depressed in groups 2 and 3 compared with the control group. The time constant of relaxation was prolonged in the three groups with aortic valve disease. No differences in early and late peak filling rate were observed in the four study groups, but the constant of myocardial stiffness increased in groups 2 and 3.
  
  
  CONCLUSIONS
  In aortic valve disease, changes in collagen architecture are associated with altered systolic function and passive diastolic properties. The sole increase in total collagen volume fraction without a change in architecture leaves systolic and passive diastolic function unaltered.
authors.affiliation: Department of Internal Medicine, University Hospital, Zurich, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Villari
authors.forename: S E
authors.initials: SE
authors.lastname: Campbell
authors.forename: O M
authors.initials: OM
authors.lastname: Hess
authors.forename: G
authors.initials: G
authors.lastname: Mall
authors.forename: G
authors.initials: G
authors.lastname: Vassalli
authors.forename: K T
authors.initials: KT
authors.lastname: Weber
authors.forename: H P
authors.initials: HP
authors.lastname: Krayenbuehl
mesh_terms: D000328:Adult; D000368:Aged; D001022:Aortic Valve Insufficiency / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D001024:Aortic Valve Stenosis / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D001706:Biopsy; D006328:Cardiac Catheterization; D002932:Cineangiography; D003094:Collagen / Q000032:analysis*; D004695:Endocardial Fibroelastosis / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D017379:Hypertrophy, Left Ventricular / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D008875:Middle Aged; D009200:Myocardial Contraction; D009210:Myofibrils / Q000737:chemistry*; D012720:Severity of Illness Index; D013314:Stress, Mechanical; D013318:Stroke Volume; D013997:Time Factors; D016277:Ventricular Function, Left / Q000502:physiology*
chemical_list: D003094:Collagen