The synthesis of alternating alpha,beta-oligodeoxyribonucleotides with alternating (3'-->3')- and (5'-->5')-internucleotic linkages as potential therapeutic agents.
A simplified synthesis of alpha-2'-deoxyribonucleoside derivatives has been developed and the solid-phase preparation of antisense alpha,beta-oligodeoxyribonucleotides with alternating (3'-->3')- and (5'-->5')-internucleotidic phosphodiester linkages, targeted against HIV-1 tat mRNA, has been accomplished. Hybridization properties of these oligonucleotide analogues have been investigated.
Koga
M
M
Division of Hematologic Products, Food and Drug Administration, Bethesda, MD 20892.
Geyer
S J
SJ
Regan
J B
JB
Beaucage
S L
SL
D001483:Base Sequence; D015696:Gene Products, tat / Q000235:genetics; D015756:Genes, tat; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000494:pharmacology; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D016376:Oligonucleotides, Antisense; D054322:tat Gene Products, Human Immunodeficiency Virus