title: A simplified method for phenotyping alpha-2-HS-glycoprotein.
abstract: Alpha-2-HS-glycoprotein is considered a reliable genetic marker for paternity testing and forensic investigation. We describe a simple, semiautomated method for phenotyping this protein in serum samples, bloodstains and dental pulp using isoelectric focusing methods followed by immunofixation techniques. This method produces clear results even with very old biological evidence.
authors.affiliation: Department of Legal Medicine, University Complutense, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: López-Abadía
authors.forename: J M
authors.initials: JM
authors.lastname: Ruiz de la Cuesta
mesh_terms: D001798:Blood Proteins / Q000032:analysis / Q000235:genetics*; D001801:Blood Stains; D003782:Dental Pulp / Q000737:chemistry; D005819:Genetic Markers; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D010334:Paternity; D010641:Phenotype; D060749:alpha-2-HS-Glycoprotein
chemical_list: C556354:AHSG protein, human; D001798:Blood Proteins; D005819:Genetic Markers; D060749:alpha-2-HS-Glycoprotein