Protection of BALB/c mice against methicillin-resistant Staphylococcus aureus infection by intraperitoneal administration of nucleoside-nucleotide mixture.
The effect of intraperitoneally administered nucleic acid components (nucleoside-nucleotide mixture) on the recovery from methicillin-resistant Staphylococcus aureus (MRSA) strain 8985N infection was studied in mice. Two experiments were conducted in which BALB/c mice were fed a nucleotide-free 20% casein diet for 30 days. On the 10th day, the mice were inoculated intravenously with viable MRSA organisms. The mice were intraperitoneally administered nucleoside-nucleotide mixture or saline (control) daily from the onset of the experiment (experiment 1) or from the day of inoculation (experiment 2). The survival rate in the nucleoside-nucleotide group in experiment 1 (70%) was significantly higher than that in the saline group (20%) (p < 0.05). In experiment 2, the survival rate in the nucleoside-nucleotide group (55%) tended to be higher than that in the saline group (36%) without statistically significant difference; furthermore, in the surviving mice, the viable MRSA organisms recovered from the spleen and the kidney were fewer in the former group than in the latter group (p < 0.05). The studies showed that the intraperitoneal administration either before or after the MRSA challenge of the nucleoside-nucleotide mixture was effective for the recovery of the mice from the infection.
Adjei
A A
AA
School of Health Sciences, University of the Ryukyus, Okinawa, Japan.
Matsumoto
Y
Y
Kina
T
T
Takamine
F
F
Yonabaru
M
M
Asato
L
L
Yokoyama
H
H
Imamura
T
T
Yamamoto
S
S
D000818:Animals; D001835:Body Weight; D015169:Colony Count, Microbial; D005260:Female; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000382:microbiology; D016106:Methicillin Resistance; D051379:Mice; D008807:Mice, Inbred BALB C; D009705:Nucleosides / Q000008:administration & dosage / Q000627:therapeutic use*; D009711:Nucleotides / Q000008:administration & dosage / Q000627:therapeutic use*; D009924:Organ Culture Techniques; D009929:Organ Size; D013154:Spleen / Q000382:microbiology; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
D009705:Nucleosides; D009711:Nucleotides