title: [Proteins, interacting with the promotor of the immunoglobulin kappa gene].
abstract: Two octanucleotide binding protein factors (mOct-1 and mOct-2A) are detected in nuclear extracts of lymphoid NS/0 cell line. These proteins, corresponding to human Oct-1 and Oct-2A nuclear factors, were purified by ion exchange and heparin-agarose chromatography. With retardation assay technique it was shown that mOct-2A factor at all steps of purification contains tightly bound protein, dissociating in the presence of a nonspecific native DNA. This octa-protein-associated factor (OAF) has low affinity to DNA and is not able to bind specifically the promoter of kappa-gene. However it interacts with DNA together with mOct-2A in highly specific manner but does not form complexes with mOct-1 and mOct-2B factors. Since OAF can be attached specifically only to mOct-2A (which play an essential role in initiation of kappa-gene transcription) this attachment may be considered as necessary for its recognition as a specific transcription factor. The ability of OAF like alpha TIF factor (specifically interacting with Oct-1) to discriminate between the Oct proteins, recognizing the same cis-acting oct-elements, shows how the non-DNA-binding transcription factors can influence differentially the transcription by two proteins binding to the same DNA sequences.
authors.forename: D V
authors.initials: DV
authors.lastname: Kaznadzeĭ
authors.forename: N N
authors.initials: NN
authors.lastname: Luchina
authors.forename: A G
authors.initials: AG
authors.lastname: Stepchenko
authors.forename: O L
authors.initials: OL
authors.lastname: Polianovskiĭ
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004586:Electrophoresis; D051863:Host Cell Factor C1; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; C497693:Hcfc1 protein, mouse; D051863:Host Cell Factor C1; D007145:Immunoglobulin kappa-Chains; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human; C494166:Pou2f1 protein, mouse; C489206:Pou2f2 protein, mouse; D014157:Transcription Factors; D004247:DNA
vernacular_title: Belki, vzaimodeĭstvuiushchie s promotorom kappa-gena immunoglobulina.