title: Mucosal and systemic T helper cell function after intragastric colonization of adult mice with Candida albicans.
abstract: In 85% of adult DBA/2 mice inoculated intragastrically with Candida albicans, significant numbers of yeast cells were recovered from the gastrointestinal tract for up to 4 weeks, with the animals eventually clearing infection in the absence of systemic disease despite the occurrence of localized, self-limiting foci of mucosal involvement in their stomachs. Two major findings in colonized mice were defective production of IgA, interleukin (IL)-4, and IL-5 by Peyer's patches lymphocytes and increased numbers of interferon-gamma-producing T cells in mesenteric lymph nodes and spleens. Relatively low levels of circulating antibodies of T helper type 2 (Th2)-dependent isotypes were also found in colonized mice, which exhibited strong footpad responses and increased resistance to systemic reinfection. Unlike systemic challenge, gastrointestinal colonization of adult immunocompetent DBA/2 mice with C. albicans appears to be an effective stimulus for the systemic development of protective Th1 responses.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bistoni
authors.forename: E
authors.initials: E
authors.lastname: Cenci
authors.forename: A
authors.initials: A
authors.lastname: Mencacci
authors.forename: E
authors.initials: E
authors.lastname: Schiaffella
authors.forename: P
authors.initials: P
authors.lastname: Mosci
authors.forename: P
authors.initials: P
authors.lastname: Puccetti
authors.forename: L
authors.initials: L
authors.lastname: Romani
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D000908:Antibodies, Fungal; D016207:Cytokines