[Identification of latent HBV infection in primary hepatic carcinoma and liver cirrhosis with polymerase chain reaction].
In order to explore latent. HBV infection in primary hepatic carcinoma (PHC) and liver cirrhosis (LC), serum HBV X and S gene were tested with polymerase chain reaction in 40 cases of PHC and LC with both negative HBsAg and HBeAg. The positive rates for X and S gene were much the same. The S gene-positive cases were, however, slightly more than the X gene-positive ones. In the cases of PHC and LC with all the five serum immunologic markers negative HBV DNA was found in 56.3% (9/16) and 45.5% (5/11) of the cases respectively. Of those patients with positive anti-HBs or anti-HBe, HBV DNA was found in 4/8 and 1/4 of the cases respectively. It was shown that in the cases of PHC and LC with all the five markers-negative, about half of them seems to be still related with HBV infection. Some of the PHC and LC cases with positive anti-HBs or anti-HBe may also have low level of viral replication. Detection of HBV DNA with multiple genes is of help to identify more PHC and LC patients with latent HBV infection.
Yang
J M
JM
Department of Gastroenterology, South-west Hospital, Third Military Medical College.
Liu
W W
WW
D006528:Carcinoma, Hepatocellular / Q000382:microbiology*; D004279:DNA, Viral / Q000097:blood; D005814:Genes, Viral; D006509:Hepatitis B / Q000382:microbiology*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008103:Liver Cirrhosis / Q000382:microbiology*; D008113:Liver Neoplasms / Q000382:microbiology*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens