The determination of liposome captured volume.
Manipulating the process by which lipids assemble to form bilayer membranes has produced a myriad of protocol-dependent liposome types. For each of these systems the arrangement of bilayers is characteristic and can be described by parameters such as aqueous entrapment per mole lipid or captured volume, vesicle size distribution, the average number of lamellae per vesicle and shape. For specific applications as model systems or drug delivery systems specific characteristics are desired. Consequently over the years many techniques have evolved to better quantitate these parameters. Here we focus on and detail several methods to quantitate liposome captured volume. We also briefly describe the available methods to measure the other aforementioned physical properties and discuss their interdependency with captured volume.
Perkins
W R
WR
Liposome Company, Inc., Princeton, New Jersey 08540.
Minchey
S R
SR
Ahl
P L
PL
Janoff
A S
AS
D005614:Freeze Fracturing / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008433:Mathematics; D008854:Microscopy, Electron / Q000379:methods; D008962:Models, Theoretical; D008968:Molecular Conformation; D009994:Osmolar Concentration; D010713:Phosphatidylcholines / Q000737:chemistry*
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines