title: Mobility of human neutrophils in response to Cryptococcus neoformans cells, culture filtrate antigen, and individual components of the antigen.
abstract: The mobility of human neutrophils (PMN) in response to encapsulated or nonencapsulated Cryptococcus neoformans cells or cryptococcal culture filtrate (CneF) and its components was studied by using a 48-well modified Boyden chamber. Encapsulated C. neoformans (isolate 184A) cells and CneF-184A stimulated directed migration of human PMN in the absence of serum (direct chemotactic activity) and activated a heat-labile component(s) in fresh human serum to become a chemoattractant(s) for human PMN (indirect chemotactic activity). At a 1:8 dilution (0.25 mg of carbohydrate per ml), CneF-184A displayed chemokinetic activity when assessed with a checkerboard assay. Nonencapsulated C. neoformans isolate 602 cells did not have direct chemotactic activity but did have indirect chemotactic activity. The capsule of C. neoformans is composed predominantly of glucuronoxylomannan (GXM). Purified GXM displayed both direct and indirect chemotactic activity. CneF-184A contains, in addition to GXM, a concanavalin A-binding mannoprotein (MP), whereas CneF-602 contains no GXM but does contain MP. CneF-184A showed direct chemotactic activity and CneF-602 did not. Both CneF-184A and CneF-602 displayed indirect chemotactic activity for human PMN. In addition, purified MP from CneF-184A, like CneF-602, showed only indirect chemotactic activity. These results indicate that GXM contributes to the direct chemotactic activity of PMN observed with the whole encapsulated yeast cells and the unfractionated CneF derived from the encapsulated cells. Both MP and GXM from encapsulated C. neoformans cells mediate indirect chemotactic activity on human PMN.
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Science Center, Oklahoma City 73190.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Dong
authors.forename: J W
authors.initials: JW
authors.lastname: Murphy
mesh_terms: D000946:Antigens, Fungal / Q000302:isolation & purification; D002465:Cell Movement / Q000276:immunology / Q000502:physiology; D002630:Chemotactic Factors / Q000096:biosynthesis; D002634:Chemotaxis, Leukocyte / Q000276:immunology* / Q000502:physiology; D003455:Cryptococcus neoformans / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000502:physiology; D011134:Polysaccharides / Q000276:immunology; D012995:Solubility
chemical_list: D000946:Antigens, Fungal; D002630:Chemotactic Factors; D008562:Membrane Glycoproteins; D011134:Polysaccharides; C025363:mannoproteins; C027478:glucuronoxylomannan