Lyophilization can generate artifacts in chromatographic profiles of inositol phosphates.
Lyophilized extracts of [3H]inositol-labelled rat heart or renal tubule preparations were found to contain unidentified 3H-labelled compounds in addition to the inositol phosphates. The appearance of these labelled substances was caused by the presence in the extracts of compounds which bound [3H]inositol when lyophilized together with it. These studies demonstrate a previously undescribed source of [3H]inositol-labelled compounds which can complicate chromatographic profiles of inositol phosphates. These problems can be overcome either by not lyophilizing the samples or by lyophilizing in the presence of 0.3 M urea, which prevents the association with [3H]inositol and does not interfere with the chromatography.
Woodcock
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EA
Cellular Biochemistry Laboratory, Baker Medical Research Institute, Prahran, Australia.
Anderson
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KE
Land
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SL
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005612:Freeze Drying; D007295:Inositol Phosphates / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D007679:Kidney Medulla / Q000737:chemistry; D008297:Male; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014508:Urea
D007295:Inositol Phosphates; D014508:Urea