Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene.
In mouse mammary tumors, the Wnt-3 gene can be activated by proviral insertion. Here we report on the isolation of a human homolog, WNT3. A genomic clone was isolated by use of mouse Wnt-3 sequences as a probe, after which cDNA containing most of the protein-encoding domain of the human gene was obtained by PCR. Comparison between the deduced mouse and human WNT-3 protein sequences showed four changes in 333 amino acids. WNT3 is located on chromosome 17q21. The gene was not found to be amplified or rearranged in a collection of human breast tumors.
Roelink
H
H
Howard Hughes Medical Institute, Department of Developmental Biology, Beckman Center, Stanford University, California 94305.
Wang
J
J
Black
D M
DM
Solomon
E
E
Nusse
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics; D011519:Proto-Oncogenes; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D051153:Wnt Proteins; D060508:Wnt3 Protein
D018076:DNA, Complementary; D011506:Proteins; C555507:WNT3 protein, human; D051153:Wnt Proteins; D060508:Wnt3 Protein; C555508:Wnt3 protein, mouse