title: Cloning and structural analysis of a human thymidylate synthase pseudogene splitted by several Alu sequences.
abstract: An unidentified genomic DNA fragment of 2.4kb that is weakly hybridizable with thymidylate synthase (TS) cDNA was cloned from a human genomic DNA library. Sequencing of the cloned DNA fragment and comparison of the sequence with that of the known human TS cDNA revealed that the DNA fragment contained a human TS processed pseudogene with unusual features. Based on the rate of nucleotide substitutions for neutral mutations in the 3'-untranslated regions between the gene and the pseudogene, it was estimated that the human TS pseudogene was formed about 16 million years ago.
authors.affiliation: Laboratory of Genetic Engineering, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takeishi
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Isono
authors.forename: N
authors.initials: N
authors.lastname: Horie
mesh_terms: D005075:Biological Evolution; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D011544:Pseudogenes; D012091:Repetitive Sequences, Nucleic Acid; D013940:Thymidylate Synthase / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D013940:Thymidylate Synthase