title: Four 9-kDa proteins excreted by somatic embryos of grapevine are isoforms of lipid-transfer proteins.
abstract: Four 9-kDa small extracellular proteins produced by embryogenic cultures in the absence of auxin have been purified from the extracellular medium of grapevine somatic embryo cultures through cation-exchange chromatography and hydrophobic-interaction chromatography. The partial amino-acid sequences reflect high similarities between the four proteins as well as with the sequences established for carrot, spinach, millet and maize nonspecific lipid-transfer proteins. All these sequences show conservation of three cysteines at positions 4, 14 and 30-32, as well as glycine, valine, tyrosine and lysine residues at positions 5, 7, 17 and 37, respectively. In-vitro lipid-transfer assays reveal that the four proteins catalyze the transfer of phosphatidylcholine from liposomes towards mitochondria with an efficiency similar or higher than that of a purified maize lipid-transfer protein.
authors.affiliation: Institut des Sciences Végétales, CNRS, Gif sur Yvette, France.
authors.forename: P
authors.initials: P
authors.lastname: Coutos-Thevenot
authors.forename: T
authors.initials: T
authors.lastname: Jouenne
authors.forename: O
authors.initials: O
authors.lastname: Maes
authors.forename: F
authors.initials: F
authors.lastname: Guerbette
authors.forename: M
authors.initials: M
authors.lastname: Grosbois
authors.forename: J P
authors.initials: JP
authors.lastname: Le Caer
authors.forename: M
authors.initials: M
authors.lastname: Boulay
authors.forename: A
authors.initials: A
authors.lastname: Deloire
authors.forename: J C
authors.initials: JC
authors.lastname: Kader
authors.forename: J
authors.initials: J
authors.lastname: Guern
mesh_terms: D000595:Amino Acid Sequence; D052179:Antigens, Plant; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005638:Fruit / Q000737:chemistry*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; C511920:lipid transfer proteins, plant