Rapid diagnosis of coccidioidomycosis with a DNA probe to ribosomal RNA.
BACKGROUND
Although nucleic acid probe hybridization assays were previously exclusively used as a tool in the research setting, such assays have recently become commercially available for the detection of a variety of infectious microorganisms.


METHODS
We used a commercially available DNA hybridization probe test that targets organism-specific ribosomal RNA sequences to rapidly diagnose a patient with disseminated coccidioidomycosis. The natural amplification inherent to such DNA:RNA probe systems obviates the need for electrophoretic separation and amplification steps, which are often required in more traditional DNA:DNA probe assays. With this probe, culture confirmation was obtained within 48 hours after the clinical specimens were received.


CONCLUSIONS
Rapid DNA hybridization probe techniques have wide application in infectious diseases, especially those characterized by slow culture growth of pathogens such as deep fungi and atypical mycobacteria.
Beard
J S
JS
Dermatology Service, Walter Reed Army Medical Center, Washington, DC.
Benson
P M
PM
Skillman
L
L
D000038:Abscess / Q000382:microbiology; D000328:Adult; D003045:Coccidioides / Q000235:genetics*; D003047:Coccidioidomycosis / Q000175:diagnosis* / Q000382:microbiology; D015342:DNA Probes; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008159:Lumbar Vertebrae / Q000382:microbiology; D008172:Lung Diseases, Fungal / Q000175:diagnosis; D008297:Male; D012331:RNA, Fungal / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*; D013122:Spinal Diseases / Q000382:microbiology
D015342:DNA Probes; D012331:RNA, Fungal; D012335:RNA, Ribosomal