Cleavages of the O- and C-glucosyl bonds of anthrone and 10,10'-bianthrone derivatives by human intestinal bacteria.
A strictly anaerobic bacterium, Bifidobacterium sp. SEN, capable of hydrolyzing the O-glucosyl of sennosides was isolated from human feces. The bacterium stepwisely hydrolyzed sennoside B to sennidin B through sennidin-8-monoglucoside in PYF medium but not in GAM broth. Addition of D-glucose to PYF medium resulted in loss of the hydrolyzing activity in culture but addition of D-fructose did not affect the activity. Coculture of this bacterium with Peptostreptococcus intermedius led to rapid accumulation of rhein anthrone in the medium. Similarly, a bacterium, Eubacterium sp. BAR, capable of cleaving the C-glucosyl of barbaloin was isolated from human feces. This bacterium grew in PYF medium containing barbaloin and produced enzyme(s) that cleave(s) the C-glucosyl. The induction of the enzymes was completely inhibited in the presence of D-glucose. Nojirimycin inhibited the enzyme activity induced by barbaloin but it did not inhibit the bacterial growth in the presence of D-glucose.
Hattori
M
M
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University.
Akao
T
T
Kobashi
K
K
Namba
T
T
D000504:Aloe; D000873:Anthracenes / Q000378:metabolism*; D000880:Anthraquinones / Q000378:metabolism*; D001644:Bifidobacterium / Q000378:metabolism*; D002400:Cathartics / Q000378:metabolism*; D005051:Eubacterium / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005959:Glucosidases / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D005960:Glucosides / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007422:Intestines / Q000382:microbiology*; D010462:Peptostreptococcus / Q000378:metabolism; D010946:Plants, Medicinal; D012676:Senna Extract; D000081226:Sennosides
D000873:Anthracenes; D000880:Anthraquinones; D002400:Cathartics; D005960:Glucosides; D000081226:Sennosides; C045601:barbaloin; D012676:Senna Extract; D005959:Glucosidases