title: Comparison of methods for specific depletion of ATP in Salmonella typhimurium.
abstract: Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or alpha-methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP-dependent processes from processes dependent on other nucleotides.
authors.affiliation: Department of Microbiology, Loma Linda University School of Medicine, California 92350.
authors.forename: M S
authors.initials: MS
authors.lastname: Johnson
authors.forename: B L
authors.initials: BL
authors.lastname: Taylor
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001149:Arsenates / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D001431:Bacteriological Techniques; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008757:Methylglucosides / Q000494:pharmacology; D009154:Mutation; D009711:Nucleotides / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D019810:Sodium Azide
chemical_list: D001149:Arsenates; D001386:Azides; D008757:Methylglucosides; D009711:Nucleotides; D006639:Histidine; C027020:methylglucoside; D000255:Adenosine Triphosphate; D019810:Sodium Azide; C025657:arsenic acid