Effective long range mapping in Schizosaccharomyces pombe with the help of swi5.
The switching gene swi5 has a function in mating-type switching. In addition, the swi5 mutation causes an increased radiation sensitivity and reduces meiotic recombination about ten-fold. Based on the latter property, an experimental protocol was developed for using swi5 in long-range mapping in S. pombe. It is suitable for a speedy mapping of any new gene which has not yet been cloned. The procedure was used to clarify the map positions of some genes.
Schmidt
H
H
Institut für Genetik, Technische Universität Braunschweig, Germany.
D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005812:Genes, Switch; D006720:Homozygote; D012568:Schizosaccharomyces / Q000235:genetics*