Platelet-activating-factor-induced augmentation of production of eosinophil-lineage cells in hematopoietic precursor cells obtained from human umbilical cord blood.
Platelet-activating factor (PAF)-induced augmentation of spontaneous differentiation into eosinophil-lineage cells from hematopoietic precursor cells was found among human umbilical cord blood mononuclear cells (CBMCs). This ability of PAF to augment eosinophil production was significantly (p < 0.01) inhibited by the addition of the anti-PAF agent WEB 2086. This enhancing effect of PAF was significantly (p < 0.01) diminished by the presence of antibodies to interleukin (IL)-1 alpha, -beta and IL-3, whereas antibodies to IL-5 or granulocyte-macrophage colony-stimulating factor (GM-CSF) did not diminish it. IL-1 beta, IL-3 or IL-5 gene expression was detected in cellular RNA isolated from both medium- and PAF-stimulated CBMCs using the reverse transcription polymerase chain reaction (RT-PCR) method; however, no expression of GM-CSF was detected. Moreover, 5 times as much IL-3 was detected in the PAF-stimulated CBMC culture supernatant than in that of cells treated with the medium. In the case of IL-1 beta, there was no difference between the 2 cell preparations. On the other hand, no IL-5 or GM-CSF was detected in the supernatant after stimulation with medium or PAF. Depletion of CD2+, CD16+ or CD19+ cells, but not CD14+ cells, from CBMCs resulted in a marked decrease in PAF-induced augmentation of eosinophil production. These results suggest that PAF induces the augmentation of IL-3 production in CBMCs which in turn enhances differentiation into eosinophil-lineage cells.
Saito
H
H
Clinical Research Center for Rheumato-Allergology, National Hospital of Sagamihara, Kanagawa, Japan.
Koshio
T
T
Yanagihara
Y
Y
Akiyama
K
K
Shida
T
T
D001381:Azepines / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000166:cytology*; D005312:Fetal Blood; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D007377:Interleukin-3 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014230:Triazoles / Q000494:pharmacology
D001381:Azepines; D016207:Cytokines; D007377:Interleukin-3; D010972:Platelet Activating Factor; D012333:RNA, Messenger; D014230:Triazoles; C052518:WEB 2086