Characteristics of Vero cytotoxin producing Escherichia coli associated with intestinal colonization and diarrhea in calves.
Isolates of Escherichia coli which produce Vero cytotoxin (VTEC) were obtained during 1983-1989 from calves raised in 5 north-central states of the USA. All of the calves experienced intestinal epithelial colonization by VTEC, diarrhea or both; twelve of the calves had bloody diarrhea. Twenty one isolates were serogroup O111 and the others were O103, O69, O45, 026, O5, or non-typable (4 isolates). All but one of the isolates hybridized with the CVD419 probe which identifies most VTEC strains. Thirty two isolates hybridized with the VT1 probe, 3 with both the VT1 and VT2 probes, and one with neither probe. The culture filtrate of the VT probe negative isolate was partially neutralized by SLT I monoclonal antibody. For the other isolates, the results of toxin neutralization by anti-SLT I and anti-SLT II monoclonal antibodies corresponded exactly with the VT1 and VT2 probe hybridization results. Three of the strains adhered in a localized manner to HEp-2 cells and Intestine 407 cells.
Dorn
C R
CR
Department of Veterinary Preventive Medicine, Ohio State University, Columbus 43210.
Francis
D H
DH
Angrick
E J
EJ
Willgohs
J A
JA
Wilson
R A
RA
Collins
J E
JE
Jenke
B H
BH
Shawd
S J
SJ
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003603:Cytotoxins / Q000096:biosynthesis; D015342:DNA Probes; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000145:classification / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D007422:Intestines / Q000382:microbiology; D009693:Nucleic Acid Hybridization / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D003603:Cytotoxins; D015342:DNA Probes; D022622:Shiga Toxin 1