[Preparative isolation of the glycosylated protein and the major core protein of bovine leukemia virus].
A technique for purifying intact bovine leukemia virus (BLV) and a procedure for preparing gag protein (p24) and envelope glycoprotein (gp 51) from intact BLV are described. The preparation method employs solubilization of BLV by n-octyl-beta-D-Glycopyranoside and sodium desoxycholate with following ultracentrifugation in a linear 10-60% sucrose gradient. The isolated components preserve their antigenicity.
Dikinene
N P
NP
Nivinskas
G G
GG
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D015683:Gene Products, gag / Q000276:immunology / Q000302:isolation & purification*; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D001909:Leukemia Virus, Bovine / Q000302:isolation & purification*; D012756:Sheep; D012995:Solubility; D014461:Ultracentrifugation; D014758:Viral Core Proteins / Q000276:immunology / Q000302:isolation & purification*; D014759:Viral Envelope Proteins / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral; D015683:Gene Products, gag; D006023:Glycoproteins; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins
Preparativnoe vydelenie glikozilirovannogo belka i osnovnogo vnutrennego belka virusa leĭkoza krupnogo rogatogo skota.