The secondary structure of milk proteins and their biological function.
A brief overview is given of the methods of determining and predicting secondary structure in proteins. The secondary structures of the milk serum proteins, lactoferrin, alpha-lactalbumin, lysozyme, and beta-lactoglobulin, as determined by x-ray crystallography, are compared with the results of a joint prediction method. This comparison evaluates critically the degree of success achieved and helps define what can reasonably be expected from a prediction in the absence of a known structure. The value of supplementary information from spectroscopic methods and the use of templates and sequence information from related proteins in improving the confidence of predictions are illustrated. One point that emerges is the general overprediction of helix content by the joint prediction method such that, for an all-beta protein such as beta-lactoglobulin, the method of Garnier, Osguthorpe, and Robson, applied with the correctly selected decision constants, provides a somewhat better approach. Secondary structure of the caseins can be predicted with less confidence than for globular proteins, and the results should be interpreted as evidence of a propensity to form transient secondary structures of the indicated type in view of the generally open and flexible conformation of caseins in solution.
Sawyer
L
L
Department of Biochemistry, University of Edinburgh, Scotland.
Holt
C
C
D000595:Amino Acid Sequence; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D007768:Lactalbumin / Q000737:chemistry / Q000378:metabolism; D007781:Lactoferrin / Q000737:chemistry / Q000502:physiology; D007782:Lactoglobulins / Q000737:chemistry / Q000378:metabolism; D008894:Milk Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry / Q000502:physiology; D017433:Protein Structure, Secondary
D002364:Caseins; D007782:Lactoglobulins; D008894:Milk Proteins; D007768:Lactalbumin; D009113:Muramidase; D007781:Lactoferrin