Bone morphogenetic protein-induced muscle- and synovium-derived cartilage differentiation in vitro.
Muscle- and synovium-derived mesenchymal-type cells differentiated into cartilage in response to a coating of partially purified rabbit bone morphogenetic protein (BMP) on a substratum of plastic. Observed by means of phase contrast microscopy, light microscope histochemistry, and electron microscopy, the sequence of events on a BMP-coated plastic substratum is the same as that observed on a substratum of bone matrix. The plastic eliminates the possibility of any endogenous allogeneic matrix-derived BMP contaminating the system. The differentiation of cartilage from synoviocytes and subsynovial cells resembles the pathologic process occurring in human chondromatosis.
Iwata
H
H
Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.
Ono
S
S
Sato
K
K
Sato
T
T
Kawamura
M
M
D000818:Animals; D001846:Bone Development; D019485:Bone Morphogenetic Proteins; D002356:Cartilage / Q000502:physiology* / Q000648:ultrastructure; D002454:Cell Differentiation; D002478:Cells, Cultured; D006133:Growth Substances / Q000494:pharmacology*; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009132:Muscles / Q000166:cytology* / Q000187:drug effects; D011506:Proteins / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects
D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins