Interactions between active-site serine beta-lactamases and so-called beta-lactamase-stable antibiotics. Kinetic and molecular modelling studies.
The interactions between imipenem and four monobactams and three class A beta-lactamases have been studied in detail. Despite their reputation as being beta-lactamase-stable, some of these compounds were significantly hydrolysed by the enzymes. The results obtained with the Streptomyces albus G beta-lactamase have been analysed in the light of molecular modelling studies. The discussion is extended to include other so-called beta-lactamase-stable antibiotics to demonstrate that this appellation can often be misleading.
Matagne
A
A
Laboratoire d'Enzymologie, Université de Liège, Belgium.
Lamotte-Brasseur
J
J
Frère
J M
JM
D000192:Actinomycetales / Q000201:enzymology; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001398:Aztreonam / Q000031:analogs & derivatives / Q000378:metabolism; D001665:Binding Sites; D007700:Kinetics; D008958:Models, Molecular; D012694:Serine; D013302:Streptomyces / Q000201:enzymology; D001618:beta-Lactamases / Q000737:chemistry / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D012694:Serine; C044145:carumonam; D001618:beta-Lactamases; D001398:Aztreonam