Evaluation of monoclonal antibodies with specificity for the human platelet HPA-1 allotypes.
Two mouse monoclonal antibodies have been characterized. Both antibodies specifically recognized the amino acid sequences which are responsible for the presence of the HPA-1a and HPA-1b alloantigens at the surface of human platelet. The monoclonality allied with an exquisite specificity of these antibodies allow a perfect typing of platelet HPA-1 status. A fast and reliable Elisa assay which fulfills all the needs and requirements of hematology laboratories has been set up and is described here. The Elisa assay has been tested on a population of 689 blood donors. The results agreed with the known phenotypic frequencies of the HPA-1 alloantigens.
Diaz
M
M
Laboratoire d'Hématologie INSERM U217, Département de Biologie, Moléculaire et Structurale, Grenoble, France.
Ryckewaert
J J
JJ
Masson
D
D
Bensa
J C
JC
Marguerie
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D016824:Antigens, Human Platelet / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006801:Humans; D039661:Integrin beta3; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D010641:Phenotype
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D010446:Peptide Fragments; C083451:human platelet antigen 1b