title: Cloning of a gene required for the alkaliphily of alkaliphilic Bacillus sp. strain C-125.
abstract: An alkali-sensitive mutant was obtained from alkaliphilic Bacillus sp. strain C-125. A DNA fragment required for the alkaliphily was cloned from the chromosomal DNA of strain C-125 by using the alkali-sensitive mutant as a host. The nucleotide sequence of the cloned DNA fragment indicated the occurrence of several ORFs. Comparison of these ORFs with sequences in the available databases revealed no closely related sequences.
authors.affiliation: Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Aono
authors.forename: A
authors.initials: A
authors.lastname: Hayakawa
authors.forename: M
authors.initials: M
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Kaneko
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016366:Open Reading Frames
chemical_list: D004269:DNA, Bacterial