Translocation junctions cluster at the distal short arm of chromosome 1 (1p36.1-2) in human neuroblastoma cells.
Human neuroblastoma cells show at high frequency structural changes of the distal short arm of chromosome 1 (1p). The commonly altered region has been identified in previous loss-of-heterozygosity (LOH) studies to involve deletion of 1p36.1-2. These bands are also the site of constitutional alterations in patients with neuroblastoma. In an approach to define the 1p36.1-2 alterations in more detail we here employ four neuroblastoma cell lines to map translocation breaks involving 1p36.1-2 by fluorescence in situ hybridization (FISH). A chromosomal interval flanked by loci DIS96 and DIS98 contained translocation junctions in each of four lines. This analysis identifies in 1p a restricted genomic region as involved in chromosomal rearrangement in different neuroblastomas. The specificity of neuroblastoma translocation junctions at the molecular level implicates this genomic region in tumor development.
Barker
P E
PE
Department of Cytogenetics, German Cancer Research Center, Heidelberg.
Savelyeva
L
L
Schwab
M
M
D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D015321:Gene Rearrangement; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D005810:Multigene Family / Q000235:genetics*; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured