Efficient photodynamic action of Victoria blue BO against the human leukemic cell lines K-562 and TF-1.
Photodynamic induced cytotoxicity by Victoria blue BO (VB-BO), merocyanine 540 (MC540), Nile blue A (NB) and 4-tetrasulfonatophenyl-porphyrin (4-TSPP) has been studied on two human leukemic cell lines: K-562 and TF-1. Cells were incubated with dyes and irradiated with different doses of white light. Cell survival was assessed by propidium iodide (PI) staining using flow cytometry analysis. Concentrations of 5 x 10(-8) M VB-BO were found to kill 75% of cells, and a concentration of 1 x 10(-7) M induced more than 99% of cell killing. To obtain the same cytotoxic level, the presence of 2.6 x 10(-5) M of MC540 during irradiation was needed. Under the conditions used, NB was ineffective as a photosensitizer, although uptake studies showed that this dye was taken by the cells in much greater amounts than any other studied dye. Cell cycle distribution of TF-1 cells, surviving MC540 or VB-BO photosensitization has been studied by flow cytometry analysis after staining with Hoechst 33342 and PI. It was found that cells in G1 phase were slightly more resistant toward MC540- and VB-BO-mediated photosensitization than cells in other phases of the cell cycle.
Fiedorowicz
M
M
Laboratoire d'Activation, Mécanime, Modélisation Moléculaire: AMP-URA CNRS 1411, Faculté des Sciences Saint Jérôme, Marseille, France.
Galindo
J R
JR
Julliard
M
M
Mannoni
P
P
Chanon
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007938:Leukemia; D010078:Oxazines / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology; D011166:Porphyrins / Q000494:pharmacology; D011744:Pyrimidinones / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D010078:Oxazines; D017319:Photosensitizing Agents; D011166:Porphyrins; D011744:Pyrimidinones; D000644:Quaternary Ammonium Compounds; C008619:Nile Blue; C084160:Victoria blue BO; C016136:tetraphenylporphine sulfonate; C003954:merocyanine dye