Affinity gel electrophoresis of nucleic acids. The interaction between water soluble polymers having malachite green and double-stranded DNAs.
Copolymers prepared by the free radical copolymerization of acrylamide and vinyl malachite green were immobilized in an agarose gel matrix to produce a novel affinity gel for double-stranded DNAs. Relative electrophoretic mobilities of calf thymus DNA and poly(dA-dT)2 strongly depended on the amount of vinyl malachite green to show the strong interaction between DNAs and the copolymers. But any affinity interaction was not observed between poly(dG-dC)2 and the copolymers.
Inami
Y
Y
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Nakamura
K
K
Ishiba
H
H
Kishida
A
A
Akashi
M
M
D020106:Acrylamide; D000178:Acrylamides; D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D011108:Polymers; D012394:Rosaniline Dyes / Q000737:chemistry*; D014867:Water
D000178:Acrylamides; D011108:Polymers; D012394:Rosaniline Dyes; D014867:Water; C005095:malachite green; D020106:Acrylamide; D004247:DNA