Human complement factor B: functional properties of a recombinant zymogen of the alternative activation pathway convertase.
The human complement factor B is a centrally important component of the alternative pathway activation of the complement system. Here we report the isolation, characterization and eukaryotic expression of the first full length cDNA transcript for human factor B. In a factor B dependent haemolysis assay, the recombinant human factor B generated by transient COS cell transfection was shown to reconstitute haemolytic activity of factor B depleted human serum. To study the biological activities assigned to factor B, the availability of recombinant polypeptides representing definite portions of the human factor B molecule is desirable.
Schwaeble
W
W
First Medical Clinic, University Hospital, Mainz, Germany.
Lüttig
B
B
Sokolowski
T
T
Estaller
C
C
Weiss
E H
EH
Meyer zum Büschenfelde
K H
KH
Whaley
K
K
Dippold
W
W
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D050577:Complement C3-C5 Convertases / Q000235:genetics* / Q000378:metabolism; D011415:Complement Factor B / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015941:Complement Hemolytic Activity Assay; D003170:Complement Pathway, Alternative; D004247:DNA / Q000302:isolation & purification; D004792:Enzyme Precursors / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D004792:Enzyme Precursors; D011994:Recombinant Proteins; D004247:DNA; D050577:Complement C3-C5 Convertases; D011415:Complement Factor B