Induced-fit association between DNA and esperamicin. Importance of structural flexibility in host DNA duplex.
In this study, a series of synthetic oligonucleotide duplexes are tested as a substrate for esperamicin. The duplexes contain a typical binding sequence of esperamicin, 5'-GGA/TCC, but have different flexibilities in helix structure from each other. When cleavage activities of these oligonucleotides by esperamicin were estimated by using DNA sequencing method, a substantial increase of the cleavage at 3'-NAGG was observed with increasing the helix flexibility. This observation indicates that structural flexibility of host DNA duplex is important in an induced-fit association between esperamicin and DNA.
Uesugi
M
M
Institute for Chemical Research, Kyoto University, Japan.
Kusakabe
T
T
Sugiura
Y
Y
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D053281:Enediynes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D053281:Enediynes; D004247:DNA; C047543:esperamicin A1