Comparing steady counterflow separation with differential chromatography.
Separation of closely related solutes by steady solid-fluid counterflow is compared with differential separation in a fixed chromatographic bed. Analogous expressions for exit concentration and mean residence time in the two systems are presented. A counterpart to chromatographic resolution is derived for binary steady counterflow separations. Estimated counterflow savings in product-concentration dilution, solvent volume requirement and solid-phase volume requirement obtained with these expressions relative to comparable chromatographic operations are compared with experimental results from adsorptive, simulated moving beds. Analysis of a size-exclusion protein separation suggests counterflow substantially decreases solvent and resin usage relative to conventional, batch operation.
Roper
D K
DK
Department of Chemical Engineering, University of Wisconsin-Madison 53706.
Lightfoot
E N
EN
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification; D012997:Solvents
D011506:Proteins; D012997:Solvents