title: Some relations between light transmission changes and biochemical parameters of stored platelet packs.
abstract: In the present work a computerised method for continuous monitoring of light transmission through platelet packs was evaluated. On series (Series 1) of 50 platelet concentrates was studied over a 5-day period, and thereafter the pH, extracellular lactate dehydrogenase (LDH) and platelet factor 4 (PF4) in the concentrates were determined. A second series (Series 2) of 52 concentrates was also monitored over 5 days, and thereafter the pH, extracellular LDH and intracellular concentrations of ATP, ADP and AMP were determined. 11 and 14 of the concentrates of Series 1 and 2, respectively, demonstrated long lasting transmission increases (> or = 3.0 days). In the total of 102 platelet concentrates 34 were observed to have late (< 1.0 day) or no transmission increase. It is concluded, that the present optical method identifies both a group of platelet concentrates having long lasting transmission increases and a group consisting of platelet concentrates having late or no transmission increase. Compared to the latter concentrates preparations with long lasting transmission changes demonstrated significant biochemical alterations. These observations could be applicable in the quality control of platelet concentrates.
authors.affiliation: Blood Transfusion Centre, Orebro Medical Center Hospital, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Järemo
authors.forename: B
authors.initials: B
authors.lastname: Edlund
authors.forename: O
authors.initials: O
authors.lastname: Berseus
authors.forename: J
authors.initials: J
authors.lastname: Kutti
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001792:Blood Platelets / Q000528:radiation effects*; D001793:Blood Preservation; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008027:Light; D010978:Platelet Factor 4 / Q000378:metabolism*
chemical_list: D010978:Platelet Factor 4; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase