Detection of specific serum IgE in clonorchiasis cases and analysis of Clonorchis sinensis allergens.
Specific serum IgE levels of Clonorchis sinensis in infected humans were measured by avidin-biotin ELISA, and allergens from C. sinensis were identified by immunoblot and autoradiography. Then, allergens fractionated by Sephadex G-200 gel filtration were analyzed, and cross-reactive allergenic components of C. sinensis reacted with paragonimiasis sera were revealed. Fourteen out of 15 C. sinensis egg-positives were found to be serum IgE positive (absorbance > 0.27). Of 14 IgE-reacting allergen bands visualized, major allergens of 66, 61.5, 45, 37, 28.5, 23.5 and 15.5 KD were recognized by more than 50% of the sera of infected humans. The considerable individual variations of IgE immune responses to C. sinensis allergenic components were also noticed. C. sinensis extract was separated into 5 fractions by Sephadex G-200 gel filtration. Seventy-four KD allergen was recognized in the first fraction, 50, 45, 37, 29.5 and 28.5 KD in the third, and 15.5 KD in the fourth. Cross-reactive allergens with sera of paragonimiasis cases were identified as 66, 45, 28.5, 13 and 7.5 KD.
Yong
T S
TS
Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea.
Kim
D S
DS
Lee
S Y
SY
Im
K I
KI
Lee
K Y
KY
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D003003:Clonorchiasis / Q000276:immunology*; D003004:Clonorchis sinensis / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*
D000485:Allergens; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007073:Immunoglobulin E