title: Cytogenetic studies of epithelial ovarian carcinoma.
abstract: We performed cytogenetic studies of 36 human epithelial ovarian carcinomas using in situ culture and robotic harvest. We obtained analyzable metaphases of all 36 tumors (100%). One or more chromosomally abnormal clones were observed in 80% of tumors. Common clonal chromosome gains (each occurring in six or more cases) included +1, +2, +3, +6, +7, +9, and +12. Common clonal chromosome losses (occurring in 12 or more cases) included -X, -4, -8, -11, -13, -15, -17, and -22. Common clonal structural abnormalities (occurring in four or more cases) involved regions 1p36, 1q32, 1q42, 3p13-->p26, 3q26-->q29, 7p22, 9q34, 11p13-p15, 17q21-->q23, 19p13.3, and 19q13.3. Trisomy 12 was noted as the sole anomaly in three of five borderline and grade 1 tumors. Two grade 2 tumors contained i(1q), -14, -15 and -22. The results suggest that the pathogenesis of borderline and low-grade tumors may differ from that of higher grade tumors. Two high-grade tumors had an apparent translocation between 17q21 and 19p13.3, two chromosome regions believed to be critical to ovarian carcinogenesis.
authors.affiliation: Division of Laboratory Genetics, Mayo Clinic, Rochester, MN 55905.
authors.forename: R B
authors.initials: RB
authors.lastname: Jenkins
authors.forename: D
authors.initials: D
authors.lastname: Bartelt
authors.forename: P
authors.initials: P
authors.lastname: Stalboerger
authors.forename: D
authors.initials: D
authors.lastname: Persons
authors.forename: R J
authors.initials: RJ
authors.lastname: Dahl
authors.forename: K
authors.initials: K
authors.lastname: Podratz
authors.forename: G
authors.initials: G
authors.lastname: Keeney
authors.forename: L
authors.initials: L
authors.lastname: Hartmann
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D018262:Adenocarcinoma, Clear Cell / Q000235:genetics; D002288:Adenocarcinoma, Mucinous / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018269:Carcinoma, Endometrioid / Q000235:genetics; D002869:Chromosome Aberrations; D018284:Cystadenocarcinoma, Serous / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics*