Isolation and characterization of nonhistone proteins associated with DNase-II hypersensitive sites of Sarcoma-180 chromatin.
Limited digestion (2 min) of Sarcoma-180 nuclei by DNase-II released two nonhistone proteins from the hypersensitive sites of chromatin. The apparent molecular weights of these two proteins were 34 and 21 kDa. These proteins showed a moderate but specific inhibition in in vitro cell free transcription assay with native chromatin as template as opposed to no effect on native DNA transcription.
Tripathi
P K
PK
Department of Biochemistry, Chittaranjan National Cancer Institute, Calcutta, India.
Panda
C K
CK
Pal
S P
SP
Roychoudhury
J
J
Choudhury
K
K
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000502:physiology*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D051381:Rats; D012510:Sarcoma 180 / Q000235:genetics*; D014158:Transcription, Genetic / Q000502:physiology
D002843:Chromatin; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II