title: Bisected N-acetylglucosamine residue of biantennary sugar chains and high-molecular-weight oligosaccharides of neuroblastoma cell membranes.
abstract: The carbohydrate moieties of glycoproteins in plasma membrane are known to correlate with the induction of cell differentiation in some kinds of cells. We investigated the asparagine-linked sugar chains of neuroblastoma and ganglioneuroma cell membranes. The acidic oligosaccharides were bi-, tri-, and tetraantennary complex-type sugar chains with Man alpha-->(Man alpha)Man beta-->GlcNAc beta-->(+/- Fuc alpha-->)GlcNAc as their cores. A comparative study of the oligosaccharides of these cells showed that the biantennary complex-type sugar chain with bisecting N-acetylglucosamine residues increased and high-molecular-weight oligosaccharides decreased in ganglioneuroma cells. It is suspected that asparagine-linked sugar chains correlate with the differentiation stage of neuroblastoma.
authors.affiliation: Department of Pediatrics, Gifu University School of Medicine, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Motoyoshi
authors.forename: N
authors.initials: N
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Orii
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000737:chemistry; D002675:Child, Preschool; D005260:Female; D005729:Ganglioneuroma / Q000737:chemistry* / Q000601:surgery; D006801:Humans; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000737:chemistry* / Q000601:surgery; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010234:Paraganglia, Nonchromaffin
chemical_list: D009844:Oligosaccharides; D000117:Acetylglucosamine