title: Effects of danazol on endometrial DNA synthesis in rats.
abstract: The suppressive effects of Danazol, an isoxazol derivative of the synthetic steroid 17 alpha-ethinyltestosterone, on endometrial DNA synthesis were investigated in rats by immunohistochemistry with bromodeoxyuridine (BrdU) and DNA-synthesizing enzyme assays. Rats treated with Danazol for 14 days at 17-19 weeks of age showed a decrease of plasma gonadotropins associated with ovarian hypofunction, persistent diestrus, and a smaller number of corpora lutea in ovary, resulting in no BrdU-immunoreactive (S-phase) cells in endometrial epithelium and lower activity of thymidine kinase in the uterus compared with control.
authors.affiliation: Medical Research Institute, Tokyo Medical and Dental University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Sakamoto
authors.forename: H
authors.initials: H
authors.lastname: Kudo
authors.forename: S
authors.initials: S
authors.lastname: Sassa
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiura
authors.forename: K
authors.initials: K
authors.lastname: Kuwa
authors.forename: N
authors.initials: N
authors.lastname: Kasahara
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000311:Adrenal Glands / Q000033:anatomy & histology; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D003613:Danazol / Q000494:pharmacology*; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004971:Estrus / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000097:blood; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000033:anatomy & histology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013937:Thymidine Kinase / Q000378:metabolism; D013940:Thymidylate Synthase / Q000378:metabolism; D014599:Uterus / Q000033:anatomy & histology
chemical_list: D007986:Luteinizing Hormone; D004247:DNA; D013940:Thymidylate Synthase; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine; D003613:Danazol