Involvement of oxygen free radicals in the action of BN 52021 (PAF antagonist) to limit myocardial infarct size.
The ability of a PAF antagonist, BN 52021, to limit myocardial infarct size in rat was assessed. Anesthetized rats were subjected to coronary artery ligation for 72 h and infarct size was measured macroscopically using TTC staining. Systolic blood pressure and ECG were monitored. BN 52021 (2.5 and 5 mg/kg i.v.) markedly reduced the infarct size, prevented the loss of R wave, an electrophysiological parameter of ischemic injury, reduced elevated serum MDA levels and demonstrated an inhibition of luminal-enhanced chemiluminescence. There was no marked change in glutathione peroxidase activity with BN 52021 treatment measured at various time intervals after coronary artery ligation. The beneficial effect of BN 52021 on ischemic injury may be due to specific inhibition of PAF-induced activation of neutrophils and consequent decreased amount of cytotoxic reactive oxygen species.
Chopra
K
K
Department of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
Singh
M
M
Gupta
S
S
Ganguly
N K
NK
D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004224:Diterpenes; D004562:Electrocardiography / Q000187:drug effects; D005260:Female; D005609:Free Radicals; D046934:Ginkgolides; D005979:Glutathione Peroxidase / Q000378:metabolism; D006352:Heart Ventricles; D007783:Lactones / Q000494:pharmacology*; D008163:Luminescent Measurements; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000201:enzymology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains
D004224:Diterpenes; D005609:Free Radicals; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D008315:Malondialdehyde; C045856:ginkgolide B; D005979:Glutathione Peroxidase