Inhibition of hematopoietic development from embryonic stem cells by antisense vav RNA.
The vav proto-oncogene is universally and specifically expressed in hematopoietic cells. vav contains a unique array of motifs allowing the protein to function as a signal transducer and possibly as a transcription factor. Under certain in vitro culture conditions murine embryonic stem cells develop into colonies containing multiple hematopoietic lineages. In embryonic stem cell lines, constitutively expressing high levels of antisense vav transcripts through a stably integrated transgene, differentiation into hematopoietic cells is disrupted. This observation presents the first evidence that vav has a critical role in the development of hematopoietic cells from totipotent cells.
Wulf
G M
GM
Department of Medicine, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215.
Adra
C N
CN
Lim
B
B
D000818:Animals; D001483:Base Sequence; D015415:Biomarkers; D018797:Cell Cycle Proteins; D002454:Cell Differentiation; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051567:Proto-Oncogene Proteins c-vav; D011519:Proto-Oncogenes; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000235:genetics
D015415:Biomarkers; D018797:Cell Cycle Proteins; D017931:DNA Primers; D011518:Proto-Oncogene Proteins; D051567:Proto-Oncogene Proteins c-vav; D016372:RNA, Antisense; D012333:RNA, Messenger; C496619:Vav1 protein, mouse