In vitro translation of RNA from the German cockroach Blattella germanica.
The translation of mRNA within total RNA of German (Blattella germanica) cockroaches was performed using a rabbit reticulocyte lysate system. Analysis of the translation products by SDS-PAGE and combined autoradiography revealed several synthesized proteins with apparent molecular weights ranging from 20 kD to 110 kD. SDS-PAGE/Western blotting of non-radiolabeled translation products and incubation with human serum with IgE to cockroach allergens showed the presence of a 36 kD and 50 kD allergen. The confirmation of the translation of the cockroach allergens from total RNA is an important first step in the cloning of cockroach allergens.
Helm
R
R
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72202.
Cockrell
G
G
Sharkey
P
P
Brenner
R
R
Burks
A W
AW
D000485:Allergens / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D000818:Animals; D015153:Blotting, Western; D002474:Cell-Free System; D003058:Cockroaches / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E; D066298:In Vitro Techniques; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes
D000485:Allergens; D012333:RNA, Messenger; D007073:Immunoglobulin E