title: SruI restriction endonuclease from Selenomonas ruminantium.
abstract: SruI, specific restriction endonuclease, has been characterized from Selenomonas ruminantium isolated from the rumen of fallow deer. Results from the study demonstrate that S. ruminantium 18D possesses a type II restriction endonuclease, which recognizes the sequence 5'-TTT decreases AAA-3'. The recognition sequence of SruI was identified using digestions on pBR322, pBR328, pUC18, M13mp18RF, pACYC184 and lambda DNA. The cleavage patterns obtained were compared with computer-derived data. SruI recognises the palindromic hexanucleotide sequence and cleaves DNA after the third T in the sequence, producing blunt ends. The purification and characterization of restriction endonuclease SruI presented here is the first described for Selenomonas ruminantium spp. and demonstrates that this microorganism possesses a DNA-cleaving enzyme with the same specificity as DraI or AhaIII.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.
authors.forename: I
authors.initials: I
authors.lastname: Vanat
authors.forename: P
authors.initials: P
authors.lastname: Pristas
authors.forename: E
authors.initials: E
authors.lastname: Rybosovál
authors.forename: A
authors.initials: A
authors.lastname: Godány
authors.forename: P
authors.initials: P
authors.lastname: Javorský
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D004274:DNA, Recombinant / Q000378:metabolism; D003670:Deer / Q000382:microbiology; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology* / Q000302:isolation & purification
chemical_list: D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases