title: Pharmacological and immunohistochemical evidence for serotonergic modulation of cholinergic nucleus basalis neurons.
abstract: Identified electrophysiologically by low threshold bursts and transient outward rectification, cholinergic nucleus basalis neurons were recorded and labelled intracellularly in guinea-pig basal forebrain slices. By means of a triple labelling immunofluorescent technique, serotonin-immunoreactive fibres were visualized in close proximity to the soma and dendrites of the biocytin-labelled, choline acetyl transferase (ChAT)-immunoreactive cells. By bath application, 5-hydroxytryptamine (5-HT) produced a direct hyperpolarization of the identified cells which was mimicked by 5-HT1A receptor agonists, suggesting that it may inhibit the tonic firing but also modulate the low threshold bursting of the cholinergic nucleus basalis neurons.
authors.affiliation: Département de Physiologie, Centre Médical Universitaire, Geneva, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Khateb
authors.forename: P
authors.initials: P
authors.lastname: Fort
authors.forename: A
authors.initials: A
authors.lastname: Alonso
authors.forename: B E
authors.initials: BE
authors.lastname: Jones
authors.forename: M
authors.initials: M
authors.lastname: Mühlethaler
mesh_terms: D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004056:Differential Threshold / Q000187:drug effects; D004594:Electrophysiology; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008239:Lysine / Q000031:analogs & derivatives; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology / Q000502:physiology*; D013377:Substantia Innominata / Q000166:cytology / Q000201:enzymology / Q000502:physiology*
chemical_list: D012701:Serotonin; D002795:Choline O-Acetyltransferase; C013411:biocytin; D008239:Lysine