title: Intracellular calcium changes associated with cholinergic nicotinic receptor activation in cultured myenteric plexus neurones.
abstract: Intracellular free calcium concentration ([Ca2+]i) was measured in cultured explants of myenteric plexus neurones by using the fluorescent calcium indicator Indol in combination with patch-clamp techniques. The basal [Ca2+]i was 94 nM and spontaneous oscillations in the internal free calcium concentration were recorded. These oscillations were associated with bursts of action potentials triggered by spontaneous nicotinic excitatory synaptic potentials. Under voltage clamp conditions, application of the selective nicotinic agonist m-hydroxyphenylpropyl-trimethylammonium iodide (10 microM) induced an inward current and increased the intracellular free calcium concentration. We conclude that cholinergic synaptic excitatory activity provide a regular calcium entry in myenteric neurone and suggest that the nicotinic channel might be significantly permeable to calcium.
authors.affiliation: Department of Pharmacology, University College of London, U.K.
authors.forename: J
authors.initials: J
authors.lastname: Trouslard
authors.forename: R
authors.initials: R
authors.lastname: Mirsky
authors.forename: K R
authors.initials: KR
authors.lastname: Jessen
authors.forename: G
authors.initials: G
authors.lastname: Burnstock
authors.forename: D A
authors.initials: DA
authors.lastname: Brown
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004558:Electric Stimulation; D004594:Electrophysiology; D005071:Evoked Potentials; D007425:Intracellular Membranes / Q000378:metabolism*; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D009994:Osmolar Concentration; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013268:Stimulation, Chemical
chemical_list: D011978:Receptors, Nicotinic; D002118:Calcium