Shear stress increases heparin-binding epidermal growth factor-like growth factor mRNA levels in human vascular endothelial cells.
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a recently identified potent vascular smooth muscle cell (SMC) mitogen. We investigated the effect of shear stress on human HB-EGF mRNA levels in cultured human umbilical vein endothelial cells (HUVEC). In response to shear stress (8 dyne/cm2), HB-EGF mRNA levels in HUVEC increased rapidly, peaked at 3 h, and returned to near base line at 7 h. The shear stress-induced HB-EGF gene expression in HUVEC is completely blocked by 12-O-tetra-decanoylphorbol-13-acetate pre-treatment, suggesting the induction of HB-EGF is mediated by protein kinase C.
Morita
T
T
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Yoshizumi
M
M
Kurihara
H
H
Maemura
K
K
Nagai
R
R
Yazaki
Y
Y
D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015342:DNA Probes; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000235:genetics*; D005786:Gene Expression Regulation; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D010812:Physical Stimulation; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015342:DNA Probes; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D004533:Egtazic Acid; D004815:Epidermal Growth Factor; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium