A screen for inhibitors of DNA recombination: identification of two new spirostanol glycosides from Chamaedorea linearis.
Two new glycosides have been isolated from the MeOH extract of the stem wood and stem bark of an Ecuadorian plant Chamaedorea linearis, and their structures have been determined by spectroscopic means and X-ray analysis of the aglycone to be 1-O-[beta-L-fucopyranosyl-(4'-sulfate)]-25R,5 alpha-spirostane-1 beta, 3 beta-diol [1]) and 1-O-[beta-L-fucopyranosyl-(4'-sulfate)]-25R,5 alpha-spirostane-1 alpha, 3 beta-diol [2]. These compounds were identified in a screen for inhibitors of recombinational DNA repair. Cytotoxic activity was also demonstrated.
Patil
A D
AD
Department of Biomolecular Discovery, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406-0939.
Baures
P W
PW
Eggleston
D S
DS
Faucette
L
L
Hemling
M E
ME
Westley
J W
JW
Johnson
R K
RK
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000096:biosynthesis*; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004484:Ecuador; D006027:Glycosides / Q000302:isolation & purification* / Q000494:pharmacology; D007939:Leukemia L1210 / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry*; D011995:Recombination, Genetic / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013150:Spirostans / Q000302:isolation & purification* / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D013150:Spirostans; C084695:1-O-(fucopyranosyl-(4'-sulfate))-5-spirostane-1,3-diol; C084696:1-O-(2',3'-diacetylfucopyranosyl-(4'-sulfate))-5-spirostan-1-ol 3-acetate; D004247:DNA