title: In situ hybridization study of interleukin-1 beta mRNA induced by kainic acid in the rat brain.
abstract: The distribution patterns of interleukin-1 beta (IL-1 beta) mRNA in various brain regions of saline- and kainic acid-treated rats were examined using in situ hybridization technique. In normal rat brain, the signals of IL-1 beta mRNA were observed in the cerebellar Purkinje cells and in dispersed cells in the hypothalamus. In the case of the kainic acid treatment, IL-1 beta mRNA was intensely induced in the olfactory bulb, lateral septum, thalamus, hypothalamus, polymorphic layers of hippocampus, piriform cortex, amygdala, entorhinal cortex and cerebral cortex at 2 h after the injection of kainic acid. In the hypothalamic region, we observed the induction of IL-1 beta mRNA around the paraventricular hypothalamic nucleus, anterior hypothalamic area, dorsomedial and ventromedial hypothalamic nucleus, mammillary regions and arcuate nucleus. The signal of IL-1 beta mRNA was still expressed 4 h after treatment with kainic acid, less intensely than at 2 h, but above the control level. In these regions, IL-1 beta mRNA was expressed mainly in the glial cells, which were densely stained by Cresyl violet and did not contain glial fibrillary acidic protein. These results suggest that IL-1 beta is produced by a certain type of glial cells, maybe microglia, and might have regulatory functions in the central nervous system.
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yabuuchi
authors.forename: M
authors.initials: M
authors.lastname: Minami
authors.forename: S
authors.initials: S
authors.lastname: Katsumata
authors.forename: M
authors.initials: M
authors.lastname: Satoh
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004027:Diencephalon / Q000187:drug effects; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007267:Injections; D007375:Interleukin-1 / Q000235:genetics*; D007608:Kainic Acid; D008297:Male; D008636:Mesencephalon / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology; D013687:Telencephalon / Q000187:drug effects
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D012965:Sodium Chloride; D007608:Kainic Acid