IL-4 receptor expression by SAC-activated B-lymphocytes: its role in B-cell proliferation and the effect of cyclosporine (CsA), prednisolone and verapamil.
Expression of the IL-4 receptor was studied in a highly purified population of human B-lymphocytes stimulated by Staphylococcus aureus, cowan I (SAC). Flow cytometric analysis showed that incubation with SAC in the absence of detectable levels of IL-2, IL-4 and IL-6 resulted in a striking increase in cellular binding of IL-4. The SAC-stimulated B-cells responded to exogenous IL-4 by DNA synthesis. This response was unaffected by CsA or prednisolone, but was inhibited by the Ca2+ channel blocker verapamil.
Degiannis
D
D
Division of Clinical Immunology, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854.
Hornung
N
N
Luke-Gustites
D
D
Raskova
J
J
Raska
K
K
D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology*; D002455:Cell Division / Q000276:immunology; D016572:Cyclosporine / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D011239:Prednisolone / Q000494:pharmacology; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen / Q000502:physiology; D013211:Staphylococcus aureus / Q000276:immunology*; D014700:Verapamil / Q000494:pharmacology
D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen; D015847:Interleukin-4; D016572:Cyclosporine; D011239:Prednisolone; D014700:Verapamil