Radioimmunodetection of human small cell lung carcinoma xenografts in the nude rat using 111in-labelled monoclonal antibody MOC-31.
The applicability of mouse monoclonal antibody MOC-31 for in vivo radioimmunodetection of human small cell lung cancer (SCLC) was investigated in a nude rat xenograft model. MOC-31 is reactive with a 38 kD pancarcinoma membrane antigen. [111In]DTPA-MOC-31 showed good in vivo immunolocalisation to xenografted SCLC cells, whereas antigen-related uptake was low in normal rat tissues and in a control antigen-negative, human-derived tumour. Non-antigen-related uptake in the liver could be blocked by pretreatment with irrelevant antibody. It is concluded that MOC-31 can be used for radioimmunodetection of SCLC in vivo and may be suitable as a targeting device in patients.
de Jonge
M W
MW
Department of Clinical Immunology, University of Groningen, The Netherlands.
Kosterink
J G
JG
Bin
Y Y
YY
Bulte
J W
JW
Kengen
R A
RA
Piers
D A
DA
The
T H
TH
de Leij
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D001666:Binding Sites, Antibody; D018288:Carcinoma, Small Cell / Q000000981:diagnostic imaging*; D006801:Humans; D007205:Indium Radioisotopes; D008175:Lung Neoplasms / Q000000981:diagnostic imaging*; D008297:Male; D008875:Middle Aged; D016719:Radioimmunodetection; D051381:Rats; D011923:Rats, Nude; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007205:Indium Radioisotopes