Separation of rare cell subpopulations with the aid of biotin-labelled ligands.
A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are first labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10(-4). The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).
Steinitz
M
M
Department of Pathology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Livoff
A
A
Tamir
S
S
Brenner
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D001710:Biotin; D006801:Humans; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D011956:Receptors, Cell Surface / Q000032:analysis*; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011956:Receptors, Cell Surface; D001710:Biotin