Effect of antrectomy and drug-induced achlorhydria on urinary excretion of N-terminal big gastrin immunoreactivity in rats.
Immunoreactivities of urinary N-terminal big gastrin and serum C-terminal gastrin were determined in intact and antrectomized rats by radioimmunoassay using two antisera specific for N- and C-termini of big gastrin, respectively. Gel filtration of urine extract from intact rat showed a single giant peak of N-terminal big gastrin immunoreactivity eluted in a later position than 1-17 gastrin-34, indicating that N-terminal peptides smaller than 1-17 gastrin-34 are excreted in urine. Serum C-terminal gastrin concentration in antrectomized rats was about one sixth that in intact rats. Urinary excretion of N-terminal big gastrin in antrectomized rats was about one sixth that in intact rats. 2 week treatment with E3810, a proton pump inhibitor, (40 mg/kg/day, s.c.) induced urinary excretion of N-terminal big gastrin in parallel with a marked increase in serum C-terminal gastrin concentration in intact rats. Antrectomy completely prevented both the increase in urinary excretion of N-terminal big gastrin and the elevation of serum C-terminal gastrin induced by administration of E3810. There was an excellent correlation between serum concentration of C-terminal gastrin and urinary excretion of N-terminal big gastrin. These results suggest that urinary N-terminal big gastrin, which mostly originates from the gastric antrum, is a useful indicator of gastrin secretion in the rat.
Miyazaki
Y
Y
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Shinomura
Y
Y
Yasunaga
Y
Y
Higashimoto
Y
Y
Kanayama
S
S
Aoki
T
T
Okuda
Y
Y
Narita
T
T
Miwa
K
K
Miyazaki
I
I
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000126:Achlorhydria / Q000652:urine*; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D005260:Female; D005743:Gastrectomy; D005755:Gastrins / Q000097:blood / Q000652:urine*; D009853:Omeprazole / Q000031:analogs & derivatives; D010446:Peptide Fragments / Q000097:blood / Q000652:urine; D011498:Protein Precursors / Q000652:urine*; D054328:Proton Pump Inhibitors; D011706:Pyloric Antrum / Q000502:physiology*; D064750:Rabeprazole; D051381:Rats; D017207:Rats, Sprague-Dawley
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D001562:Benzimidazoles; D005755:Gastrins; D010446:Peptide Fragments; D011498:Protein Precursors; D054328:Proton Pump Inhibitors; D064750:Rabeprazole; C013706:big gastrin; D009853:Omeprazole