Simultaneous measurement of intracellular calcium and electrical activity from patterned neural networks in culture.
Multisite extracellular electrical activity and intracellular calcium were recorded simultaneously. Electrical signals were measured using microelectrode array substrates. A novel cell positioning technique was combined with a method for controlling neurite outgrowth, which allowed cell-electrode contacts to be established easily, thus facilitating the electrical recording. Intracellular calcium was measured optically using the indicator fluo 3. Under low-magnesium conditions, cultured rat cortical neurons showed periodic transients of fluo-3 fluorescence, which were synchronized with the periodic bursting observed electrically. The intervals between bursts could be determined by electrical stimulation through the substrate electrodes. The results suggest that functional synaptic connections are formed in the culture system.
Jimbo
Y
Y
NTT Basic Research Laboratories, Tokyo, Japan.
Robinson
H P
HP
Kawana
A
A
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured / Q000187:drug effects / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology; D003584:Cytological Techniques / Q000295:instrumentation; D004558:Electric Stimulation; D004594:Electrophysiology; D004622:Embryo, Mammalian; D004867:Equipment Design; D008274:Magnesium / Q000494:pharmacology; D008839:Microelectrodes; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D051381:Rats; D012666:Semiconductors
D008274:Magnesium; D002118:Calcium