Acquired resistance and persistence of Candida albicans following oral candidiasis in the mouse: a model of the carrier state in humans.
In our experimental model of oral candidiasis in the CD1 mouse, the primary infection showed reproducible Candida overgrowth kinetics with a peak level on day 5 of the infection. After day 7, the population stabilized at about 300 colony-forming units per excised mucosal tissue. The primary infection triggered an inflammatory response that resolved in under 8 days. At this point, the histological pattern of the mucosa reached a new equilibrium between recruited and resident mononuclear cells. The primary infection also rapidly stimulated cellular immunity, as measured from day 4 by a delayed-type hypersensitivity footpad reaction. Following a second topical challenge with Candida 30 days after the primary infection, the infection was barely detectable and a typical local delayed-type hypersensitivity reaction occurred between 24-72 h. It is proposed that acquired resistance, in conjunction with low-level persistence of Candida in our model, mimics the carrier state in sensitized humans.
Lacasse
M
M
Groupe de Recherche en Ecologie Buccale (GREB), Faculté de médecine dentaire, Université Laval, Québec, Canada.
Fortier
C
C
Chakir
J
J
Côté
L
L
Deslauriers
N
N
D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D002180:Candidiasis, Oral / Q000276:immunology* / Q000382:microbiology; D002353:Carrier State; D004195:Disease Models, Animal; D006968:Hypersensitivity, Delayed; D007110:Immunity, Active; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009061:Mouth Mucosa / Q000382:microbiology