Absorption of N4-D-glucopyranosylsulphamethazine by rat everted intestinal sacs.
Absorption of the N4-D-glucose conjugate of sulphamethazine (glucose-SMZ, 0.5 mM) by isolated everted sacs of the rat small intestine was studied at 37 degrees and pH 6.6. Phlorizin (0.5-2.0 mM) significantly reduced (P < 0.05) both mucosal and serosal transfer of glucose-SMZ and inhibition of mucosal transfer appeared to be concentration-dependent. Phloretin (0.5 mM) and removal of Na+ from the incubation medium also diminished absorption of glucose-SMZ. Furthermore, D-glucose (0.5 and 5.0 mM) inhibited mucosal and serosal transfer of the glycoside. The results suggest the D-glucose/Na+ cotransporter mediates absorption of glucose-SMZ from the small intestine of the rat. Thus, glucose-SMZ might be bioavailable from ingested tissues in which it is present.
Wang
Y
Y
Department of Pharmacology, University of Leeds, U.K.
Grigg
R
R
McCormack
A
A
Symonds
H
H
Bowmer
C
C
D000818:Animals; D001682:Biological Availability; D002352:Carrier Proteins / Q000378:metabolism*; D005504:Food Analysis; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D010693:Phloretin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D013418:Sulfamethazine / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis / Q000378:metabolism
D002352:Carrier Proteins; D013418:Sulfamethazine; C042106:N(4)-glucopyranosylsulfamethazine; D012964:Sodium; D005947:Glucose; D010693:Phloretin