title: Production of monoclonal anti-idiotype antibodies which mimic an M-like protein of Streptococcus equi.
abstract: Rat monoclonal anti-idiotype antibodies (mAb2) were raised against two mouse monoclonal antibodies (mAb1), 1D10 and 2A6, with specificity for the M-like protein of Streptococcus equi. The capacity of the mAb2 to inhibit the binding between the corresponding mouse mAb1 against which the mAb2 were raised and the M-like protein was investigated in an inhibition EIA. One of the ten mAb2 examined, namely 5D1 (anti-mAb1 1D10), was able to inhibit this binding. The mAb2 5D1 bound to the mAb1 1D10 in such a way as to completely inhibit the subsequent binding of the M-like protein antigen to the paratope of the mAb1 1D10. The mAb2 5D1 is likely to represent a true image of the M-like protein antigen and may thus be described as an Ab2 beta anti-idiotype antibody.
authors.affiliation: CSL Limited, Research and Development Division, Parkville, Victoria, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: Jean-Francois
authors.forename: D C
authors.initials: DC
authors.lastname: Poskitt
authors.forename: L M
authors.initials: LM
authors.lastname: MacDonald
authors.forename: S J
authors.initials: SJ
authors.lastname: Turnbull
authors.forename: D
authors.initials: D
authors.lastname: Yasmeen
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000276:immunology*; D002352:Carrier Proteins; D051379:Mice; D051381:Rats; D013291:Streptococcus / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C014689:streptococcal M protein