Diagnosis and molecular epidemiology of the African horse sickness virus by the polymerase chain reaction and restriction patterns.
African horse sickness is a viral disease caused by an orbivirus belonging to the Reoviridae family. This paper describes a polymerase chain reaction (PCR) for amplifying segments 7, which encode for VP 7, a protein common to the 9 known serotypes of this virus. A reverse transcription step is necessary before amplification. No amplified product could be observed in cell cultures infected with other equine viruses. The amplified DNAs were digested to completion by 8 different restriction enzymes. The restriction fragment length polymorphisms allowed the differentiation of the group of serotypes AHSV-1, 3, 6, 8 and the viruses AHSV-2, AHSV-4, AHSV-5, AHSV-7 and AHSV-9. Differences could also be described between vaccinal strains of the same serotype produced in cell cultures or in brains of suckling mice.
Zientara
S
S
CNEVA/Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort, France.
Sailleau
C
C
Moulay
S
S
Plateau
E
E
Crucière
C
C
D000356:African Horse Sickness Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D006736:Horses; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral