title: Purification and characterization of the E1 component of the Clostridium magnum acetoin dehydrogenase enzyme system.
abstract: In Clostridium magnum strain Wo Bd P1 the formation of the enzyme components of the acetoin dehydrogenase enzyme system E1 (acetoin:2,6-dichlorophenolindophenol oxidoreductase Ao:DCPIP OR), E2 (dihydrolipoamide acetyltransferase DHLTA) and E3 (dihydrolipoamide dehydrogenase DHLDH) were induced during growth on acetoin. Ao:DCPIP OR was purified from acetoin-grown cells in two steps by chromatography on DEAE-Sephacel and on Mono Q HR. Native Ao:DCPIP OR exhibited a M(r) of 138,000; it consisted of two different subunits of M(r) alpha 38,500 and M(r) beta 34,000, and it occurred most probably in a tetrameric alpha 2 beta 2 structure. The N-terminal amino acid sequences of the alpha- and beta-subunits revealed homologies to the N-termini of the corresponding subunits of Ao:DCPIP OR from Pelobacter carbinolicus and from Alcaligenes eutrophus; furthermore, the N-terminus of the beta-subunit exhibited homologies to the N-termini of beta-subunits from different 2-oxo acid dehydrogenases.
authors.affiliation: Institut für Mikrobiologie, Georg-August-Universität zu Göttingen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Lorenzl
authors.forename: F B
authors.initials: FB
authors.lastname: Oppermann
authors.forename: B
authors.initials: B
authors.lastname: Schmidt
authors.forename: A
authors.initials: A
authors.lastname: Steinbüchel
mesh_terms: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000093:Acetoin / Q000378:metabolism; D000094:Acetoin Dehydrogenase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000217:Acyltransferases / Q000302:isolation & purification; D000421:Alcaligenes / Q000201:enzymology; D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D003013:Clostridium / Q000201:enzymology*; D003470:Culture Media; D008058:Dihydrolipoamide Dehydrogenase / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology; D007658:Ketone Oxidoreductases; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry; D016415:Sequence Alignment
chemical_list: D003470:Culture Media; D009097:Multienzyme Complexes; D000093:Acetoin; D010088:Oxidoreductases; D000094:Acetoin Dehydrogenase; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); C066825:acetoin-2,6-dichlorophenolindophenol oxidoreductase; D008058:Dihydrolipoamide Dehydrogenase; D000217:Acyltransferases; C033588:dihydrolipoamide acyltransferase; D005947:Glucose