[Preparation of monoclonal antibodies to phenobarbital].
Monoclonal antibodies to phenobarbital have been developed. Spleen cells of BALB/c mouse immunized with conjugate keyhole limpet haemocyanin and phenobarbital were fused with P3-X63-Ag8.653 mouse myeloma cells. Three monoclonal antibodies, selected by indirect ELISA, were produced in mouse ascite fluids, purified and analyzed. Antibody 1A9 was selected for use in immunoassay, its association constant being 1.6 x 10(9) M-1.
Danilova
N P
NP
Dzgoev
A B
AB
Bekman
N I
NI
Vasilov
R G
RG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D010634:Phenobarbital / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D006241:Haptens; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D010634:Phenobarbital
Poluchenie monoklonal'nykh antitel k fenobarbitalu.