Rapid detection of numerical aberrations of chromosomes 13, 18 and 21 in chorionic mesenchymal cells.
We have devised and evaluated a rapid screening method for the detection of numerical aberrations of chromosomes 13, 18 and 21 in chorionic villus cells. We used non-radioactive in situ hybridization (ISH) with three chromosome-specific probes on overnight-attached mesenchymal cells from chorionic villi. A blind study was performed of 47 karyotypically normal samples, one triploid sample, two samples trisomic for chromosome 21, and two samples from a fetus with putative mosaicism (46/47, +21). All samples were hybridized with the chromosome 18- and 21-specific probes. Thirty samples were additionally hybridized with the chromosome 13-specific probe. The test could be completed within 3-4 days of sampling. In samples disomic with respect to the probed chromosomes, an average of 2 per cent (range 0-9 per cent) had three hybridization signals. By contrast, in the samples trisomic for the probed chromosome(s), 57 per cent (chromosome 13), 51 per cent (chromosome 18), and an average of 74 per cent (55-86 per cent) (chromosome 21) of the nuclei exhibited three signals. In the putative mosaic samples, the number of nuclei with three chromosome 21-specific signals ranged from 41 to 69 per cent. We conclude that this technique rapidly and clearly distinguishes between normal and trisomic/triploid samples, and consequently may be of use in future prenatal diagnosis.
Bryndorf
T
T
Department of Obstetrics and Gynaecology, University Hospital/Rigshospitalet, Copenhagen, Denmark.
Christensen
B
B
Xiang
Y
Y
Lind
A M
AM
Philip
J
J
D002824:Chorionic Villi / Q000648:ultrastructure; D015193:Chorionic Villi Sampling; D002869:Chromosome Aberrations; D018244:Chromosomes, Artificial, Yeast; D002882:Chromosomes, Human, Pair 13; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D015342:DNA Probes; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007621:Karyotyping; D008648:Mesoderm / Q000648:ultrastructure; D009030:Mosaicism; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011296:Prenatal Diagnosis / Q000379:methods*
D015342:DNA Probes