title: Common structural and expressional properties of vertebrate caldesmon genes.
abstract: We have determined the genomic structure of chicken caldesmon (CaD) gene. The gene, 100-150 kilobases long, is composed of 17 exons. Exons 1a-1, 1a-2, and 1a-3 encode the 5'-terminal sequence specific to the mRNAs for CaDs expressed in gizzard. Exon 1b encodes the 5'-terminal sequence of the brain l-CaD and locates downstream of exons 1a-1, 1a-2, and 1a-3. The genomic construction of the chicken CaD resembles with that of the human CaD. Exon 3 of chicken CaD gene possesses the unique structure similar to that of human CaD gene; the common domain in both h- and l-CaDs (amino acid residues 74-199 for h-CaD and residues 66-191 for l-CaD) and the central repeating domain specific to h-CaD (amino acid residues 200-419) are encoded in exons 3a and 3b, respectively. Of particular interest is that the two consensus 5'-splice sites are found in the borders between exons 3a and 3b, and exon 3b and intron. Therefore, the expressional regulation between h- and l-CaDs can be explained by selection of these 5'-splice sites. Alternative 3'-splice sites also exist at intron/exon junction of exon 14 and the difference in selection of the sites would induce the specific Ala-508 insertion in the brain l-CaD.
authors.affiliation: Department of Neurochemistry and Neuropharmacology, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Haruna
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Yano
authors.forename: O
authors.initials: O
authors.lastname: Takeuchi
authors.forename: K
authors.initials: K
authors.lastname: Sobue
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005091:Exons / Q000235:genetics; D015698:Genomic Library; D008957:Models, Genetic; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002148:Calmodulin-Binding Proteins