title: Stable assembly of PsaE into cyanobacterial photosynthetic membranes is dependent on the presence of other accessory subunits of photosystem I.
abstract: We studied assembly of the PsaE subunit of photosystem I into photosynthetic membranes of cyanobacterial mutant strains that lack specific photosystem I subunits. Radiolabeled PsaE was incubated with photosynthetic membranes, and their binding and assembly were assayed by resistance to removal by chaotropic agents and proteolytic digestion. PsaE incorporated into the wild-type membranes was resistant to these treatments. In the absence of PsaD, it was resistant to proteolytic digestion, but was removed by NaBr. When the membranes were isolated from a mutant strain in which the psaF and psaJ genes have been inactivated, PsaE assembled in vitro could not be removed. PsaE could associate with the membranes of the strain DF in which the psaD, psaJ and psaF genes have been mutated. However, the radiolabeled PsaE associated with these membranes was removed both by the proteolytic as well as by the chaotropic agents. Characterization of PsaE present in vivo revealed similar results. These observations suggest that PsaD and PsaF/J may interact with PsaE and stabilize it in the photosystem I complex.
authors.affiliation: Department of Botany, Hebrew University of Jerusalem, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Cohen
authors.forename: V P
authors.initials: VP
authors.lastname: Chitnis
authors.forename: R
authors.initials: R
authors.lastname: Nechushtai
authors.forename: P R
authors.initials: PR
authors.lastname: Chitnis
mesh_terms: D015153:Blotting, Western; D000458:Cyanobacteria / Q000201:enzymology*; D004579:Electron Transport; D046911:Macromolecular Substances; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism* / Q000648:ultrastructure; D045331:Photosystem I Protein Complex
chemical_list: D046911:Macromolecular Substances; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; C079741:photosystem I, psaE subunit