title: Generation of reactive oxygen species by blood monocytes during acute Plasmodium knowlesi infection in rhesus monkeys.
abstract: The status and kinetics of monocyte activation during acute P. knowlesi infection was investigated by latex-induced, luminol-dependent chemiluminescence (CL) response. The contribution of various reactive oxygen species (ROS) to CL response was estimated before infection and at peak parasitaemia (day 7 post infection) by using scavengers of ROS (benzoate, catalase and superoxide dismutase). The chemiluminescence index (CLI) was not found to be significantly different from controls on day 2 postinfection, but was significantly higher on days 5 and 7 postinfection. Hydroxyl radical (OH.) production was considerably elevated, whereas superoxide anion (O2-.) and hydrogen peroxide (H2O2) production dropped following infection. These changes in generation of ROS are discussed in relation to the progression of parasitaemia to high levels, immunopathology and immunosuppression during acute P. knowlesi infection.
authors.affiliation: Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Jayshree
authors.forename: N K
authors.initials: NK
authors.lastname: Ganguli
authors.forename: M L
authors.initials: ML
authors.lastname: Dubey
authors.forename: K
authors.initials: K
authors.lastname: Mohan
authors.forename: R C
authors.initials: RC
authors.lastname: Mahajan
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D019817:Benzoic Acid; D002374:Catalase / Q000494:pharmacology; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical / Q000097:blood*; D008163:Luminescent Measurements; D008253:Macaca mulatta; D008288:Malaria / Q000097:blood*; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D016790:Plasmodium knowlesi; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000097:blood*; D013997:Time Factors
chemical_list: D001565:Benzoates; D016166:Free Radical Scavengers; D013481:Superoxides; D017665:Hydroxyl Radical; D019817:Benzoic Acid; D002374:Catalase; D013482:Superoxide Dismutase