Lack of copper binding sites in ceruloplasmin of LEC rats with abnormal copper metabolism.
Recently it was found that the clinical features of the LEC rat closely resemble those of human Wilson's disease. One of the characteristics of the animal is low levels of serum ceruloplasmin. Therefore, by using LEC rats, we attempted to define molecular basis of the deficiency in active site of ceruloplasmin in Wilson's disease patients. We made 3 monoclonal antibodies, ID2 against active site of ceruloplasmin, ID1 against inactive site of ceruloplasmin, and the remaining one against metallothionein. Using these monoclonal antibodies, we examined immunohistochemical stainings of LEC rat liver tissues, and compared them with those of LEA rats, as a control. ID1 stained the hepatocytes of both LEA and LEC rats, whereas ID2 stained LEA rat hepatocytes only. The results indicated that the ceruloplasmin secreted by LEC rat hepatocytes is mostly in inactive form. The antibody against metallothionein stained LEA rat hepatocytes only. This finding may also indicate that LEC rat hepatocytes express less amount of metallothionein than those of LEA rats.
Hiyamuta
S
S
Central Research Laboratories, Idemitsu Kosan Co., Ltd., Chiba, Japan.
Takeichi
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002570:Ceruloplasmin / Q000737:chemistry / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D005260:Female; D006527:Hepatolenticular Degeneration / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008664:Metal Metabolism, Inborn Errors / Q000378:metabolism* / Q000473:pathology; D008668:Metallothionein / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains
D000911:Antibodies, Monoclonal; D003300:Copper; D008668:Metallothionein; D002570:Ceruloplasmin