title: Stimulated renal tubular epithelial cells induce anergy in CD4+ T cells.
abstract: Renal tubular epithelial cells (TEC) can express MHC class II molecules in vitro and in vivo. Their ability to also secrete cytokines and express adhesion molecules suggests a possible immune accessory role for TEC. We have previously documented that TEC process and present antigen to T cell hybridomas. However, engagement of the T cell receptor alone is sufficient to induce IL-2 secretion by T cell hybridomas. We now report that presentation of antigen by TEC to a CD4+ T cell clone results in functional inactivation of the T cells. Despite antigen-specific anergy, these T cells are viable and proliferate in response to IL-2. Furthermore, allogeneic antigen presenting cells were unable to restore the T cell proliferative response, suggesting that the mechanism(s) was not entirely costimulator-dependent.
authors.affiliation: Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: G G
authors.initials: GG
authors.lastname: Singer
authors.forename: H
authors.initials: H
authors.lastname: Yokoyama
authors.forename: R D
authors.initials: RD
authors.lastname: Bloom
authors.forename: A M
authors.initials: AM
authors.lastname: Jevnikar
authors.forename: N
authors.initials: N
authors.lastname: Nabavi
authors.forename: V R
authors.initials: VR
authors.lastname: Kelley
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000032:analysis*; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D006825:Hybridomas / Q000166:cytology; D007376:Interleukin-2 / Q000494:pharmacology; D007684:Kidney Tubules / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology*
chemical_list: D015704:CD4 Antigens; D007376:Interleukin-2