Preparation of a human monoclonal antibody derived from cervical lymph nodes of a patient with anaplastic carcinoma of the thyroid.
An anaplastic carcinoma cell line, KOA-2, was established from a 59-year-old patient with anaplastic carcinoma of the thyroid who died only one month after diagnosis in association with rapid progress of her disease. A human monoclonal antibody, 3C5 (IgM), was generated by a fusion of human B lymphoblastoid cell line, HO-323, with lymphocytes from cervical lymph nodes of the patient, who had been treated with local immunotherapy. Immunocytostaining demonstrated that 3C5 reacted with KOA-2, and membrane immunofluorescence demonstrated that 3C5 reacted with cell membranes. Immunohistochemical staining studies demonstrated selective reaction of 3C5 with three malignant tumors, anaplastic carcinoma of the thyroid (2/4 sampled tested), papillary carcinoma of the thyroid (8/12), and breast cancer (2/6), but no reaction with specimens of benign thyroid disease or normal thyroid gland was demonstrated. The monoclonal antibody and the cell line established from one patient have potential use in the analysis of carcinogenesis and applications to therapy of anaplastic carcinoma of the thyroid.
Baba
M
M
Department of Surgery II, Osaka University Medical School, Suita, Japan.
Kobayashi
T
T
Oka
Y
Y
Yagyu
T
T
Tamaki
Y
Y
Takeda
T
T
Monden
T
T
Shimano
T
T
Tsuji
Y
Y
Murakami
H
H
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002277:Carcinoma / Q000276:immunology*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology; D008875:Middle Aged; D009333:Neck; D013964:Thyroid Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M