title: New telomeres in yeast are initiated with a highly selected subset of TG1-3 repeats.
abstract: The creation of new telomeres was studied by generating a site-specific double-strand break in diploid strains of Saccharomyces cerevisiae that are unable to carry out homologous recombination. New telomere formation occurred approximately 1% of the time but only when (T2G4)13 was present proximal to the break site. About half of the healing events occurred at a number of 1- to 9-bp G or G, T sequences located as far as 128 bp distal to the T2G4 repeats. Surprisingly, in 16 events at sites ending in GTGG, the first TG1-3 nucleotides added always included either an 11- or a 13-bp sequence (GTGTGGGTGTG or GTGTGTGGGTGTG), after which each new telomere diverged into a less ordered TG1-3 pattern. Moreover, at 75% of the remaining addition sites, these same 11- or 13-bp sequences were found overlapping the junction between the chromosomal primer and the newly added sequences. We propose that short G, T sequences near an organizing sequence such as (T2G4)13 can act as primers to pair with the template RNA of a telomerase and add new sequences that are complementary to that RNA.
authors.affiliation: Rosentiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254-9110.
authors.forename: K M
authors.initials: KM
authors.lastname: Kramer
authors.forename: J E
authors.initials: JE
authors.lastname: Haber
mesh_terms: D001483:Base Sequence; D015825:Chromosomes, Fungal / Q000378:metabolism*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D006147:Guanine / Q000378:metabolism*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016615:Telomere / Q000378:metabolism*; D013941:Thymine / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D006147:Guanine; D004253:DNA Nucleotidylexotransferase; D013941:Thymine