HPA-1 typing by PCR amplification with sequence-specific primers (PCR-SSP): a rapid and simple technique.
A DNA-based method was developed to genotype donors for the human platelet antigens HPA-1a and -1b. Sequence-specific primers (SSP) were used in the polymerase chain reaction (PCR) which allowed the HPA-1a/1a, -1b/1b and -1a/1b genotypes to be determined by PCR alone, no second analytical stage was required. 10 donors were tested by PCR-SSP and the results were concordant with serological phenotyping and independent DNA analysis.
Metcalfe
P
P
Department of Haematology, St Bartholomew's Hospital and Medical College, London.
Waters
A H
AH
D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D016824:Antigens, Human Platelet; D004247:DNA