Dendritic arborization patterns of cortical interneurons labeled with the lectin, Vicia villosa, and injected intracellularly with Lucifer yellow in aldehyde-fixed rat slices.
Neurons whose cell bodies had been stained by a lectin, Vicia villosa, which recognizes terminal N-acetylgalactosamine residue, were intracellularly injected with Lucifer yellow (LY), in aldehyde-fixed slices of the parietal cortex of rats. LY was subsequently visualized immunocytochemically. All injected neurons had smooth or only sparsely spiny dendrites and resembled the various forms of gamma-aminobutyric acid-ergic neurons previously described in rat neocortex. In layers II/III, IV and V, most injected neurons were multipolar. In layer VI, most had vertically elongated dendritic fields. Some injected neurons in layer IV had an oval or vertically elongated soma and a bitufted dendritic arborization pattern. There was a gradual increase in the overall dendritic extent in deeper layers of the cortex.
Ojima
H
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Neural Systems Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
D000818:Animals; D002540:Cerebral Cortex / Q000648:ultrastructure*; D003712:Dendrites / Q000648:ultrastructure*; D005404:Fixatives; D005456:Fluorescent Dyes; D005557:Formaldehyde; D066298:In Vitro Techniques; D007395:Interneurons / Q000648:ultrastructure*; D007546:Isoquinolines; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008845:Microinjections; D037121:Plant Lectins; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar
D005404:Fixatives; D005456:Fluorescent Dyes; D007546:Isoquinolines; D037102:Lectins; D037121:Plant Lectins; D011108:Polymers; C042242:Vicia lectins; D005557:Formaldehyde; C017475:lucifer yellow; C003043:paraform