A flow-through microsampling device applied to an ion-exchange chromatography system.
A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0-9 microliters/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.
Nilsson
J
J
Department of Electrical Measurements, Lund Institute of Technology, Sweden.
Szecsi
P
P
Schafer-Nielsen
C
C
D001798:Blood Proteins / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D005782:Gels; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D046911:Macromolecular Substances; D008566:Membranes
D001798:Blood Proteins; D005782:Gels; D046911:Macromolecular Substances