Modulation of mammalian cell proliferation by a modified tRNA base of bacterial origin.
Addition of the q-base to q-deficient non-transformed mammalian cells stimulated their proliferation. The q-base also improved proliferation of some cancer-derived cell lines, but inhibited proliferation of others. The proliferation of HeLa-S3 carcinoma cells was stimulated by q under aerobic conditions, but was inhibited when the cells had shifted their energy metabolism towards glycolysis as the result of oxygen limitation. Q-deficient cells could not adapt their proliferation to the respective oxygen tension. The q-base stimulated the proliferation of non-transformed fibroblasts but inhibited proliferation of the same cell line, when aerobic glycolysis was increased after transformation with the ras gene. The results suggest that the q-base permits mammalian cells to adapt their proliferation to their specific metabolic state.
Langgut
W
W
Institut für Biochemie der Medizinischen Fakultät, Universität Erlangen-Nürnberg, Germany.
Reisser
T
T
Nishimura
S
S
Kersten
H
H
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D015687:Cell Hypoxia; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D004734:Energy Metabolism; D006019:Glycolysis; D006147:Guanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009857:Oncogenes; D012343:RNA, Transfer / Q000187:drug effects / Q000502:physiology*; D051381:Rats
D006147:Guanine; C019549:queuine; D012343:RNA, Transfer