[Some effects of pH, diuretics and organic acids on the uptake of oxalate in a renal tubular cell line (LLC-PK1)].
The effects of changes in extracellular pH and treatments with diuretics or some organic acids on the uptake of oxalate were studied in an epithelial cell line of renal origin (LLC-PK1 cells). The oxalate uptake into the cells exhibited a marked sensitivity to extracellular, pH, in the present results with acidic pH stimulating and alkaline pH inhibiting the DIDS sensitive uptakes. The uptake of oxalate was clearly inhibited by DIDS, proportionally to the concentrations from 10 microM to 1 mM. Furosemide, chlorothiazide, probenecid and acetazolamide inhibited the oxalate uptake significantly. Probenecid has the most potency on the inhibition of the uptake in high concentrations, and acetazolamide needed a high concentration for the small effect. However, allopurinol has no effect on the uptake as well as dicarboxylates (malate, succinate and phenyl succinate) and organic acids (urate and para-amino hippurate). We discussed the mechanisms of the inhibition against the oxalate uptake concerning with each drug. It was suggested that probenecid may inhibit the oxalate carriers directly, while furosemide, chlorothiazide and acetazolamide may inhibit then by the direct actions and some secondary effects which are provided by those drugs.
Ebisuno
S
S
Department of Urology, Minami Wakayama National Hospital.
Ohkawa
T
T
Schied
C
C
Menon
M
M
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D002264:Carboxylic Acids / Q000494:pharmacology*; D002478:Cells, Cultured; D004232:Diuretics / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007684:Kidney Tubules / Q000187:drug effects* / Q000378:metabolism; D010070:Oxalates / Q000493:pharmacokinetics*; D013552:Swine
D002264:Carboxylic Acids; D004232:Diuretics; D010070:Oxalates; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid