Comparison of properties of mistletoe lectin I A-chain and ricin B-chain conjugate with native toxins.
Chimeric toxin protein was prepared from the mistletoe lectin I A-chain and ricin B-chain by using the disulfide exchange reaction. Ricin and chimeric protein were indistinguishable in binding to immobilized asialofetuin in ELISA. Chimeric protein was more toxic to Jurkat cells than native mistletoe lectin I, but not so effective as native ricin. In the presence of NH4Cl, which enhances the toxicity of some toxins and immunotoxins, but does not influence ricin toxicity, both ricin and chimeric toxin had equal cytotoxic activity. The possibility is discussed that the ricin B-chain protects the ricin A-chain (RTA) from degradation during delivering RTA from the cell surface to the place where RTA is translocated into the cytosol.
Tonevitsky
A G
AG
Institute for Genetics of Microorganisms, Moscow, Russian Federation.
Toptygin AYu
Shamshiev
A T
AT
Agapov
I I
II
Ershova
E V
EV
Pfuller
U
U
Pfuller
K
K
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000633:toxicity*; D014784:Mistletoe / Q000737:chemistry*; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D010946:Plants, Medicinal; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000633:toxicity; D054790:Ribosome Inactivating Proteins, Type 2; D012276:Ricin / Q000737:chemistry / Q000633:toxicity*; D014118:Toxins, Biological / Q000737:chemistry / Q000633:toxicity*
D037102:Lectins; D037121:Plant Lectins; D028321:Plant Preparations; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum; D012276:Ricin