Multicolour fluorescence in situ hybridisation to order small, single-copy probes on metaphase chromosomes.
In constructing complete human chromosome maps, the relative order of markers and their precise chromosomal location will be combined. Multicolour in situ hybridisation, in which two probes are simultaneously hybridised to chromosomes and subsequently distinguished, potentially will provide both types of information. Using this technique, we have produced an ordered map of eight human chromosome 3 DNA markers, using small, single-copy probes that can detect target sequences ranging in size from 4 kb to as little as 500 bp.
Heppell-Parton
A C
AC
MRC Clinical Oncology and Radiotherapeutics Unit, Cambridge, UK.
Albertson
D G
DG
Fishpool
R
R
Rabbitts
P H
PH
D002478:Cells, Cultured; D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human; D002893:Chromosomes, Human, Pair 3; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007211:Indoles; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D008677:Metaphase
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI