The ubiquitous subunit of erythroid transcription factor NF-E2 is a small basic-leucine zipper protein related to the v-maf oncogene.
Erythroid transcription factor NF-E2 is a tissue-restricted heterodimeric protein which recognizes an extended AP-1 motif [(T/C)TGCTGA(C/G)TCA(T/C)] found in the upstream locus control regions of the alpha- and beta-globin gene clusters. A cDNA clone encoding a cell-type-specific subunit of NF-E2, designated p45 NF-E2, has previously been characterized and shown to encode a basic-leucine zipper DNA-binding protein. Here we describe protein purification and cloning of cDNA that encodes the second basic-leucine zipper subunit of the native NF-E2 heterodimer. This polypeptide, designated p18, is widely expressed. It displays extensive homology to the v-maf oncogene product and a human retinal-specific protein, NRL. Unusual features in the basic region shared by v-Maf, NRL, and p18 place them in a distinct subfamily of AP-1-like proteins.
Andrews
N C
NC
Division of Hematoloy/Oncology, Children's Hospital, Dana-Farber Cancer Institute, Boston, MA.
Kotkow
K J
KJ
Ney
P A
PA
Erdjument-Bromage
H
H
Tempst
P
P
Orkin
S H
SH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry; D050981:Erythroid-Specific DNA-Binding Factors; D005136:Eye Proteins; D005914:Globins / Q000235:genetics; D006801:Humans; D016350:Leucine Zippers / Q000235:genetics; D004915:Leukemia, Erythroblastic, Acute; D046911:Macromolecular Substances; D050985:MafK Transcription Factor; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; D009838:Oligodeoxyribonucleotides; D051256:Oncogene Protein v-maf; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000737:chemistry; D009857:Oncogenes; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry; D014162:Transfection; D014407:Tumor Cells, Cultured; D014764:Viral Proteins; D016335:Zinc Fingers / Q000235:genetics
D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D005136:Eye Proteins; C494695:MAFK protein, human; D046911:Macromolecular Substances; D050985:MafK Transcription Factor; C494696:Mafk protein, mouse; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; C494692:NFE2 protein, human; C490037:NRL protein, human; C494693:Nfe2 protein, mouse; C084884:Nrl protein, mouse; D009838:Oligodeoxyribonucleotides; D051256:Oncogene Protein v-maf; D009856:Oncogene Proteins, Viral; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D014764:Viral Proteins; C495606:v-Maf protein, Avian retrovirus AS42; D005914:Globins