[The production of clones of "man x Chinese hamster" hybrid cells containing different parts of the human genome].
Some approach has been described to create hybrid cell lines (human x Chinese hamster) which contain different parts of human genome, and then efficiently to reveal and isolate the human DNA from these. This method involves the introduction of a selective marker in different sites of the human cell genome, by transfecting them with plasmid SV2neo, and the use of flow cytometry and DNA polymerase chain reaction with primers specific only for human DNA.
Filatov
M V
MV
Bulat
S A
SA
Drobchenko
E A
EA
Kotlovanova
L V
LV
Pantina
R A
RA
Semenova
E V
EV
Stepanov
S I
SI
Tret'iakov
A N
AN
Shcherbakova
O G
OG
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology; D005434:Flow Cytometry; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology*; D008168:Lung; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D014162:Transfection
D004247:DNA
Poluchenie klonov gibridnykh kletok "chelovek x kitaĭskiĭ khomiachok", soderzhashchikh raznye chasti genoma cheloveka.