Primordial germ cells in the primitive streak stages chick embryo as studied by scanning electron microscopy.
Chick embryo primordial germ cells (PGCs) at stages 4-8 were localised in the late hypoblast layer. PAS staining confirmed their distribution in a large arc at the anterior border of the area pellucida/area opaca. By scanning electron microscopy the PGCs were seen as spherical cells within the late hypoblast layer. Individual cells separated from this layer and collected in groups of 2-10 in shallow pockets in the hypoblast layer prior to their migration. The pockets containing PGCs were clearly visible by light and scanning electron microscopy and demarcated the germinal ridge at stages 4-8.
England
M A
MA
Department of Anatomy, University of Leicester, UK.
Matsumura
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G
D000818:Animals; D002469:Cell Separation; D002642:Chick Embryo / Q000648:ultrastructure*; D004707:Endoderm / Q000648:ultrastructure; D005775:Gastrula / Q000648:ultrastructure; D005854:Germ Cells / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D010503:Periodic Acid-Schiff Reaction