Immunocytochemical detection of bromodeoxyuridine in tissues of the toad Bufo arenarum Hensel.
We present a modified protocol for the immunocytochemical identification of 5-bromo-2'-deoxyuridine (BrdU) as an indicator of cell replication in different tissues of the toad, Bufo arenarum Hensel. Animals were sacrificed 60 min after BrdU (5 mg/100 g body weight) was injected into the dorsal lymph sac. The tissues were fixed in Carnoy's fluid and stained by the immunoperoxidase method using an anti-BrdU monoclonal antibody. This protocol can be used safely for the study of cell replication in toads and other Anura amphibia.
Gagliardino
J J
JJ
Center of Experimental and Applied Endocrinology, School of Medical Sciences, National University of La Plata, Argentina.
Gómez Dumm
C L
CL
Bianchi
C E
CE
Luna
G C
GC
D000818:Animals; D001973:Bromodeoxyuridine / Q000032:analysis* / Q000493:pharmacokinetics; D002022:Bufo arenarum / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000737:chemistry / Q000378:metabolism; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D008297:Male; D016196:S Phase / Q000502:physiology; D014018:Tissue Distribution
D001973:Bromodeoxyuridine