title: Crystallization and preliminary X-ray studies on the core proteins of cellobiohydrolase I and endoglucanase I from Trichoderma reesei.
abstract: The catalytic core domains of cellobiohydrolase I (CBHI) and endoglucanase I (EGI) from Trichoderma reesei have been crystallized using the hanging drop vapour diffusion method. In the case of CBHI, use of polyethylene glycol 20,000, and calcium chloride at low pH produced good quality single crystals suitable for X-ray studies. The crystals belong to a primitive orthorhombic space group with unit cell dimensions a = 84.0 A, b = 86.2 A, c = 111.8 A, and diffract beyond 2.0 A resolution. Bipyramidal crystals of EGI core were grown from ammonium sulphate at pH 7.5. The crystals are tetragonal, either P4(1)22 or the enantiomorph P4(3)22, with cell dimensions a = b = 101.8 A and c = 198.0 A, and at best diffract to a resolution of 2.5 A.
authors.affiliation: Department of Molecular Biology, Uppsala University Biomedical Centre, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Divne
authors.forename: I
authors.initials: I
authors.lastname: Sinning
authors.forename: J
authors.initials: J
authors.lastname: Ståhlberg
authors.forename: G
authors.initials: G
authors.lastname: Pettersson
authors.forename: M
authors.initials: M
authors.lastname: Bailey
authors.forename: M
authors.initials: M
authors.lastname: Siika-aho
authors.forename: E
authors.initials: E
authors.lastname: Margolles-Clark
authors.forename: T
authors.initials: T
authors.lastname: Teeri
authors.forename: T A
authors.initials: TA
authors.lastname: Jones
mesh_terms: D000645:Ammonium Sulfate; D002122:Calcium Chloride; D043366:Cellulose 1,4-beta-Cellobiosidase; D003460:Crystallization; D018360:Crystallography, X-Ray; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D007202:Indicators and Reagents; D011092:Polyethylene Glycols; D011487:Protein Conformation; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D007202:Indicators and Reagents; D011092:Polyethylene Glycols; D006026:Glycoside Hydrolases; D043366:Cellulose 1,4-beta-Cellobiosidase; D002122:Calcium Chloride; D000645:Ammonium Sulfate