title: Molecular rearrangement of the ALL-1 gene in acute myeloid leukemia without cytogenetic evidence of 11q23 chromosomal translocations.
abstract: Translocations which involve chromosome band 11q23 are frequently found in infants and adults with acute myeloid leukemia (AML) or acute lymphoblastic leukemia. We previously cloned a gene called ALL-1 which spans the 11q23 breakpoint and is rearranged in most cases of leukemia with 11q23 abnormalities. In the present report, we have investigated the occurrence of ALL-1 rearrangement in cases of AML without cytogenetic evidence of 11q23 abnormalities. We detected molecular rearrangements of the ALL-1 gene in 3 of 4 patients with de novo AML and trisomy 11 as a sole chromosomal abnormality. Furthermore, we found DNA rearrangements of ALL-1 in 2 of 19 patients with de novo AML and normal cytogenetics. We conclude that molecular rearrangement of ALL-1 often can be detected in de novo AML, despite the absence of cytogenetic abnormalities involving 11q23.
authors.affiliation: Division of Hematologic Oncology and Bone Marrow Transplantation, Roswell Park Cancer Institute, Buffalo, New York 14263.
authors.forename: M A
authors.initials: MA
authors.lastname: Caligiuri
authors.forename: S A
authors.initials: SA
authors.lastname: Schichman
authors.forename: M P
authors.initials: MP
authors.lastname: Strout
authors.forename: K
authors.initials: K
authors.lastname: Mrózek
authors.forename: M R
authors.initials: MR
authors.lastname: Baer
authors.forename: S R
authors.initials: SR
authors.lastname: Frankel
authors.forename: M
authors.initials: M
authors.lastname: Barcos
authors.forename: G P
authors.initials: GP
authors.lastname: Herzig
authors.forename: C M
authors.initials: CM
authors.lastname: Croce
authors.forename: C D
authors.initials: CD
authors.lastname: Bloomfield
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D002880:Chromosomes, Human, Pair 11; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014178:Translocation, Genetic / Q000235:genetics*