title: [In vitro adherence of Pasteurella haemolytica to tracheal mucins and a tracheal epithelial cell preparation from cattle].
abstract: Outer membrane protein preparations (OMP) of 11 Pasteurella haemolytica field isolates of the serovars A1, A2, A11 and non-typable strains from cattle were extracted by N-lauryl-sarcosine sodium salt. Capsular extracts were prepared by heat treatment. Both preparations and whole cell suspensions bound to a preparation of an epithelial cell wall fraction of trachea and to tracheal mucus of cattle. Binding was demonstrated by enzyme linked immunosorbent assay (ELISA). Distinct high binding values were shown by the OMP and the capsular extracts of the serovar A1-strains.
authors.affiliation: Institut für Mikrobiologie und Tierhygiene, Fachbereich Veterinärmedizin, Freie Universität Berlin.
authors.forename: L
authors.initials: L
authors.lastname: Bötcher
authors.forename: I
authors.initials: I
authors.lastname: Stöter
authors.forename: E
authors.initials: E
authors.lastname: Hellmann
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D002417:Cattle; D002473:Cell Wall / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D006168:Guinea Pigs; D016978:Mannheimia haemolytica / Q000378:metabolism*; D009077:Mucins / Q000378:metabolism*; D014132:Trachea / Q000166:cytology / Q000382:microbiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D009077:Mucins
vernacular_title: In vitro Adhärenz von Pasteurella haemolytica an Trachealmucin und einer Trachealepithelzellpräparation vom Rind.