Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes.
Cyclin dependent kinases (CDK's) are kinases that interact with cyclins and regulate cell division. Genomic clones encoding human CDK2, CDK4, and CDK5 were obtained and mapped to their respective chromosomal loci using fluorescence in situ hybridization on human lymphocyte metaphase spreads. Interestingly, CDK2 and CDK4 were located at the same position, 12q13, and CDK5 was mapped to 7q36. 12q13 has been shown to be associated with chromosome alterations such as amplifications and translocations in solid tumors. 7q36 does not appear to be a major site of chromosome alterations in tumors. As CDK2 and CDK4 appear to be important in regulating the human cell cycle, it is possible that the alterations of the 12q13 locus in tumors may involve changes in the regulation of CDK2 and CDK4 genes.
Demetrick
D J
DJ
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724.
Zhang
H
H
Beach
D H
DH
D042846:CDC2-CDC28 Kinases; D002453:Cell Cycle / Q000235:genetics*; D002455:Cell Division; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002897:Chromosomes, Human, Pair 7; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008677:Metaphase; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins
D016213:Cyclins; D011518:Proto-Oncogene Proteins; D011494:Protein Kinases; D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; C495900:CDK4 protein, human; C495905:CDK5 protein, human; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases