zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly.
cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon-COP), zeta-COP exists in both coatomer bound and free pools.
Kuge
O
O
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.
Hara-Kuge
S
S
Orci
L
L
Ravazzola
M
M
Amherdt
M
M
Tanigawa
G
G
Wieland
F T
FT
Rothman
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D016466:CHO Cells; D002352:Carrier Proteins / Q000096:biosynthesis*; D002417:Cattle; D002850:Chromatography, Gel; D002966:Clathrin / Q000096:biosynthesis*; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism* / Q000648:ultrastructure; D008099:Liver / Q000378:metabolism*; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D020743:rhoB GTP-Binding Protein
D002352:Carrier Proteins; D002966:Clathrin; D017931:DNA Primers; D018076:DNA, Complementary; D046911:Macromolecular Substances; D008565:Membrane Proteins; D016376:Oligonucleotides, Antisense; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019204:GTP-Binding Proteins; D020743:rhoB GTP-Binding Protein