title: Vibriophage D10 contains non-permuted DNA with cohesive ends.
abstract: Phage D10, a Vibrio cholerae O-1 El Tor group X phage, is one of the five newly isolated phages used in the phage typing scheme developed for V. cholerae O-1 biotype El Tor and belongs to the Myoviridae family. From electron microscopic studies it is shown that phage D10 has a DNA genome of 32 +/- 0.2 kb. This is the first report where it has been shown by the construction of a partial denaturation map that this vibriophage genome is nonpermuted and has cohesive ends. The location of the ends of the DNA in the phage head has also been inferred.
authors.affiliation: Department of Biochemistry, University College of Science, Calcutta, India.
authors.forename: B K
authors.initials: BK
authors.lastname: Chakrabarti
authors.forename: D J
authors.initials: DJ
authors.lastname: Chattopadhyay
authors.forename: A N
authors.initials: AN
authors.lastname: Ghosh
mesh_terms: D004279:DNA, Viral / Q000235:genetics / Q000648:ultrastructure*; D016679:Genome, Viral; D017900:Myoviridae / Q000235:genetics / Q000648:ultrastructure*; D009691:Nucleic Acid Denaturation; D014734:Vibrio cholerae
chemical_list: D004279:DNA, Viral