Quantitative 1H-NMR analysis of amniotic fluid.
Ten amniotic fluid samples (36-38 weeks gestation) are analysed by NMR spectroscopy. Of the species identified in the spectra, valine (mean 198 microM: SEM 57 microM), lactate (9.73 mM; 2.05 mM), alanine (689 microM: 115 microM), acetate (6.87 mM: 1.54 mM), citrate (363 microM: 59 microM), glucose (4.54 mM: 1.28 mM) indoxyl-sulphate (n = 4,270 microM), histidine (n = 6, 125 microM: 31 microM) and formate (n = 4, 92 microM) are quantified using standard addition. The factors governing the detection limits and lowest quantifiable amounts are discussed as are the extension of the work into in vivo magnetic resonance spectroscopy (MRS) in the clinic.
McGowan
P E
PE
Department of Pure and Applied Chemistry, Strathclyde University, Glasgow, UK.
Reglinski
J
J
Wilson
R
R
Walker
J J
JJ
Wisdoms
S
S
McKillop
J H
JH
D000596:Amino Acids / Q000032:analysis*; D000653:Amniotic Fluid / Q000737:chemistry*; D002264:Carboxylic Acids / Q000032:analysis*; D002585:Cesarean Section; D005260:Female; D005947:Glucose / Q000032:analysis*; D006801:Humans; D007200:Indican / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D011247:Pregnancy
D000596:Amino Acids; D002264:Carboxylic Acids; D005947:Glucose; D007200:Indican