[The use of the HET-CAM test for the determination of the irritating effects of humic acids].
Using the synthetic humic acid product HS 1500 and the native product Sodium-Humate, the suitability of the HET-CAM-test to evaluate irritating effects was tested. Neither sensitizing nor irritating properties were detectable in concentrations of up to 10% in either humic acid solution. This leads us to the conclusion that both products are very mildly acting substances on mucous membranes and skin, causing no irritative effects. In order to improve the certainty of these results, it is still currently recommended to carry out the Draize-test in addition to the HET-CAM procedure (SPIELMANN, 1992). We are convinced that the HET-CAM-test can be a very useful first step in distinguishing between irritative and non irritative substances and in protecting animals from pain, even in the event that the results of both tests do not clearly correspond to each other. Taking into account the results of our experiments, a modified HET-CAM-test also seems to be useful to predict the antiphlogistic effects of chemical substances.
Wiegleb
K
K
Institut für Pharmakologie, Pharmazie und Toxikologie, Universität Leipzig.
Lange
N
N
Kühnert
M
M
D000826:Animal Testing Alternatives; D000818:Animals; D002642:Chick Embryo / Q000187:drug effects*; D006812:Humic Substances / Q000633:toxicity*; D007509:Irritants / Q000633:toxicity*
D006812:Humic Substances; D007509:Irritants
Zur Anwendung des HET-CAM-Tests für die Ermittlung irritativer Effekte von Huminsäuren.