[Histological evaluation of brush cytology of rabbit conjunctiva].
The number of the cells and the layers of the conjunctiva which can be obtained by brush cytology was studied on rabbit conjunctiva using cytological and histological methods. About 1,000, 3,500, and 10,000 conjunctival epithelial cells can be obtained by 7, 14, and 20 brush strokes histological observations showed that all varieties of the layers including basal cells were obtained without destroying the basement membrane. This study showed that the brush cytology can offer single cell suspension only from the conjunctival epithelium, and that brush cytology is useful to investigate the conjunctival epithelium by flow cytometry, polymerase chain reaction, and other methods.
Fukagawa
K
K
Department of Ophthalmology, School of Medicine, Keio University, Shinjuku-ku, Japan.
Shimmura
S
S
Shimazaki
J
J
Tsubota
K
K
Oguchi
Y
Y
D000818:Animals; D002452:Cell Count; D003228:Conjunctiva / Q000166:cytology* / Q000648:ultrastructure; D003584:Cytological Techniques; D004847:Epithelial Cells; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry; D011817:Rabbits