Baroreceptor reflex-linked changes in catechol metabolism in the rat rostral ventrolateral medulla.
1. Using in vivo voltammetry, this study relates catecholamine metabolism within the rat rostral ventrolateral medulla to the level of mean arterial pressure (MAP) under halothane anaesthesia. 2. A vasopressor region was circumscribed with electrical stimulations in an area located 1000-1700 microns rostral to the obex. A catechol signal was then ascertained within this area. The recording site was surrounded with phenyl-N-methyl-ethanolamine transferase immuno-positive cell bodies. 3. Three levels of decrease of arterial pressure were induced with nitroprusside infusion: -15, -35 and -55 mmHg (n = 5 in each group) from baseline for 30 min. This led to increases in the catechol signal which were inversely related to the degree of hypotension (P < 10(-4) vs. saline for the 35 and 55 mmHg groups, P < 0.05 for the 35 mmHg group as compared to the 15 and 55 mmHg groups following recovery from hypotension). 4. Following sino-aortic deafferentation, nitroprusside-induced hypotension (-35 mmHg) did not lead to any change in the catechol signal in the rostral ventrolateral medulla (n = 5). Furthermore, controlled hypotension induced in intact rats did not evoke any change in the catechol signal recorded in a dopaminergic area of the midbrain, the ventral tegmental area (A10 area; n = 5). 5. An infusion of phenylephrine increased MAP by 35 mmHg from a baseline pressure of 105 mmHg for 30 min and evoked a non-significant decrease in the catechol signal (n = 5). In another group of rats a lower baseline pressure (80 mmHg) was stabilized (n = 5) with a higher concentration of halothane. An identical increment in pressure was then produced by a phenylephrine infusion and led to a significant reduction in the catechol signal (P < 0.05 vs. saline under similar conditions; n = 5). 6. The new findings of this study are that the level of activity of the metabolism of catecholamine in the rostral ventrolateral medulla (i) is continuously related to the level of arterial pressure, (ii) functions close to its resting level under baseline conditions and is primarily engaged during hypotension and (iii) is baroreflex linked. 7. Given the lack of direct evidence for a link between unit activity and catechol metabolism, these changes in catechol activity, recorded continuously in vivo next to adrenergic cell bodies, may represent the biochemical-specific counterpart of changes in the level of electrical unitary activity of presumed adrenergic cardiovascular medullospinal sympathoexcitatory neurons. Therefore, it provides evidence that adrenaline-synthesizing neurons in the rostral ventrolateral medulla respond to baroreceptor inputs.
Rentero
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Laboratoire de Physiologie de l'Environnement, Faculté de Médecine Grange-Blanche, Lyon, France.
Kitahama
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K
Quintin
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L
D000818:Animals; D017704:Baroreflex / Q000187:drug effects / Q000502:physiology*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002396:Catechols / Q000378:metabolism*; D003714:Denervation; D004558:Electric Stimulation; D004837:Epinephrine / Q000494:pharmacology; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology; D008297:Male; D008526:Medulla Oblongata / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D009474:Neurons / Q000187:drug effects; D009599:Nitroprusside / Q000494:pharmacology; D010084:Oxidation-Reduction; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012849:Sinoatrial Node / Q000033:anatomy & histology / Q000502:physiology
D002396:Catechols; D009599:Nitroprusside; D010656:Phenylephrine; D004837:Epinephrine