title: Hepatic protoporphyrin accumulation in a broiler chicken.
abstract: Hepatocellular accumulation and biliary excretion of protoporphyrin in a 6-week-old chicken are described. The affected liver was grossly dark gray to black. Histologically, hepatocytes, bile canaliculi, and bile-duct lumina contained dark-brown granules. These protoporphyrin pigment accumulations were brightly birefringent under polarized light. The ultrastructural appearance of granules revealed hair-like crystals in a radial arrangement. Extracted levels of protoporphyrin in the affected liver were determined to be 630 micrograms per gram of wet (frozen) liver. Spectrofluorometric scans of liver extracts and sedimented crystals were characteristic of protoporphyrin.
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, Russell Research Center, Athens, Georgia 30604.
authors.forename: S
authors.initials: S
authors.lastname: Hafner
authors.forename: D
authors.initials: D
authors.lastname: Bounous
authors.forename: L C
authors.initials: LC
authors.lastname: Kelley
authors.forename: R
authors.initials: R
authors.lastname: Campagnoli
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D017094:Porphyrias, Hepatic / Q000378:metabolism / Q000473:pathology / Q000662:veterinary; D011201:Poultry Diseases / Q000378:metabolism / Q000473:pathology; D011524:Protoporphyrins / Q000032:analysis / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D011524:Protoporphyrins