title: Application of enzyme-immunohistochemistry for the diagnosis of aspergillosis, candidiasis, and zygomycosis in three lovebirds.
abstract: Aspergillosis, candidiasis, and zygomycosis were diagnosed in tissues from three lovebirds (Agapornis roseicollis) using indirect enzyme-immunohistochemical techniques. In these techniques, the first antibody was raised against fungal antigen. A second antibody, which was raised in another animal species, was added to link the first antibody to enzyme-immunocomplexes. The reactivity of specific monoclonal and polyclonal antibodies was visualized by immunoreactivity of alkaline phosphatase anti-alkaline phosphatase and peroxidase anti-peroxidase immunocomplexes. All three birds examined had dermal candidiasis. In addition, one of the birds was diagnosed with concomitant acute ocular aspergillosis, and another bird was diagnosed with chronic zygomycotic myocarditis.
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Science, University of Cordoba, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Carrasco
authors.forename: M J
authors.initials: MJ
authors.lastname: Bautista
authors.forename: J M
authors.initials: JM
authors.lastname: de las Mulas
authors.forename: H E
authors.initials: HE
authors.lastname: Jensen
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000378:metabolism; D001228:Aspergillosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D001230:Aspergillus / Q000276:immunology; D001715:Bird Diseases / Q000175:diagnosis*; D001717:Birds; D002175:Candida / Q000276:immunology; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D005260:Female; D007124:Immunoenzyme Techniques; D008297:Male; D009090:Mucorales / Q000276:immunology; D009091:Mucormycosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000946:Antigens, Fungal