title: Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II.
abstract: The structure of the gene encoding the 14.5 kDa subunit of the human RNA polymerase II (or B) has been elucidated. The gene consists of six exons, ranging from 52 to over 101 bp, interspaced with five introns ranging from 84 to 246 bp. It is transcribed into three major RNA species, present at low abundance in exponentially growing HeLa cells. The corresponding messenger RNAs contain the same open reading frame encoding a 125 amino acid residue protein, with a calculated molecular weight of 14,523 Da. This protein (named hRPB14.5) shares strong homologies with the homologous polymerase subunits encoded by the Drosophila (RpII15) and yeast (RPB9) genes. Cysteines characteristic of two zinc fingers are conserved in all three corresponding sequences and, like the yeast protein, the hRPB14.5 subunit exhibits zinc-binding activity.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes (CNRS), Unité 184 de Biologie Moléculaire et de Génie Génétique (INSERM), Institut de Chimie Biologique, Strasbourg, France.
authors.forename: J
authors.initials: J
authors.lastname: Acker
authors.forename: M
authors.initials: M
authors.lastname: Wintzerith
authors.forename: M
authors.initials: M
authors.lastname: Vigneron
authors.forename: C
authors.initials: C
authors.lastname: Kedinger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D005796:Genes; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C079911:Rbp9 protein, Drosophila; D012319:RNA Polymerase II