High-performance liquid chromatographic determination of histatins in human saliva.
A simple method for the assay of histatins in human saliva by high-performance liquid chromatography has been developed. The histatins in saliva were extracted with 0.1 M HCl-methanol, and histatins 1, 3, 5, and 6 were separated and concentrations were determined by reversed-phase chromatography. This simple method enabled the determination of levels of individual histatins from the saliva of normal subjects. The average concentration of histatins 1, 3, 5, and 6 in parotid saliva collected from 26 healthy volunteers aged from 20 to 30 years were 11.25 +/- 5.65, 8.15 +/- 3.08, 7.67 +/- 3.12, and 1.56 +/- 0.53 mumol/l (mean +/- S.D.), respectively.
Sugiyama
K
K
Department of Pharmacology, Okayama University Dental School, Japan.
Ogata
K
K
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000302:isolation & purification; D000099053:Histidine-Rich Glycoprotein
D011506:Proteins; D012471:Salivary Proteins and Peptides; D000099053:Histidine-Rich Glycoprotein