Electroblotting proteins onto carboxymethylcellulose membranes for sequencing.
Proteins and peptides separated by polyacrylamide gel electrophoresis either in acetic acid-urea or in sodium dodecyl sulfate (SDS)-containing gels were electroblotted onto carboxymethylcellulose membrane and eluted from the membrane by dilute acid. Polypeptides thus recovered with a high yield can be used to get structural or biological data.
Alimi
E
E
Laboratoire de Biologie Cellulaire, Université Paris-Val de Marne, Créteil, France.
Martinage
A
A
Sautière
P
P
Chevaillier
P
P
D000595:Amino Acid Sequence; D002266:Carboxymethylcellulose Sodium; D002467:Cell Nucleus / Q000737:chemistry; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D011479:Protamines / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000302:isolation & purification; D017421:Sequence Analysis; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification; D013094:Spermatozoa / Q000737:chemistry / Q000648:ultrastructure
D006657:Histones; D008567:Membranes, Artificial; D011479:Protamines; D011506:Proteins; C046533:protamine P1; C048529:protamine P2; D012710:Serum Albumin, Bovine; D012259:Ribonuclease, Pancreatic; D002266:Carboxymethylcellulose Sodium