Identification, characterization, and biological activity of somatostatin receptors in human neuroblastoma cell lines.
To investigate the presence of biologically active somatostatin (SS) receptors in neural crest-derived tumors, radioligand binding studies, cyclic AMP accumulation, intracellular calcium, and growth assays were performed in eight human neuroblastoma (NB) cell lines. Mathematical modeling of binding experiments strongly indicates the presence of heterogeneity of sites. The first site (SSR1) is present in 40% of the NB cell lines and binds with low capacity (0.5 pmol/mg protein) and high affinity (0.1-1 nM) SS14, SS28, and analogues. The second site (SSR2) is a high capacity site (200 pmol/mg protein), widely distributed in all of the cell lines investigated, that shows relative selectivity yet low affinity (100 nM) for SS14, SS28, and [D-Trp8]SS14 without any apparent biological activity. SSR1 is coupled to a pertussis toxin-sensitive G protein, inhibits forskolin- or VIP-stimulated adenylate cyclase activity, decreases intracellular free calcium, and mediates inhibition (30%) of both DNA synthesis and cell growth. Analysis of cell cycle distribution in aphidicolin-synchronized SSR1-positive NB cells indicated that this inhibitory effect is partially mediated by a transient accumulation in G0-G1. Our data indicate high affinity binding sites for SS14, and analogues are present and biologically active in a subset of NB cells.
Maggi
M
M
Sezione di Endocrinologia, Università di Firenze, Florence, Italy.
Baldi
E
E
Finetti
G
G
Franceschelli
F
F
Brocchi
A
A
Lanzillotti
R
R
Serio
M
M
Camboni
M G
MG
Thiele
C J
CJ
D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology; D003198:Computer Simulation; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D009447:Neuroblastoma / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D015282:Octreotide / Q000494:pharmacology; D017481:Receptors, Somatostatin / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D017481:Receptors, Somatostatin; D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D015282:Octreotide; D002118:Calcium