[Intensification of genomic dactyloscopy by means of hybridization with modified oligonucleotides, forming duplexes with increased stability].
The modified oligodeoxyribonucleotide (m5C-n2A-m5C)5 containing 5-methylcytosine and 2-aminoadenine instead of cytosine and adenine residues, respectively has been used as a hybridisation probe in DNA fingerprinting. The oligonucleotide, due to the substitutions forms more stable duplexes with complementary sequence in DNA than the corresponding nonmodified pentadecanucleotide. The comparison with its natural counterpart displays considerably increased intensity of bands in patterns obtained with modified analog. The use of such analogues can increase sensitivity and shorten time of DNA fingerprinting.
Prosniak
M I
MI
Veselovskaia
S I
SI
Miasnikov
V A
VA
Efremova
E Iu
EIu
Potapov
V K
VK
Limborskaia
S A
SA
Sverdlov
E D
ED
D015075:2-Aminopurine / Q000031:analogs & derivatives / Q000032:analysis; D044503:5-Methylcytosine; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D016172:DNA Fingerprinting; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*
D009841:Oligonucleotides; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D044503:5-Methylcytosine; D003596:Cytosine
Intensifikatsiia genomnoĭ daktiloskopii putem gibridizatsii s modifitsirovannymi oligonukleotidami, obrazuiushchimi dupleksy s povyshennoĭ stabil'nostiu.