Blood transfusion as a means for transmission of retrovirus-induced lymphoproliferative disease in mice.
Lymphoproliferative disease was elicited in C57BL/6KH and (BALB/c x C57BL/6)F1 hybrids by a single intraperitoneal injection of 10(5) FFU of LP-BM5 virus preparation. The disease could reproducibly be transferred by a single intravenous transfusion of 0.2 ml of whole blood as well as 0.1 ml of blood cells, plasma or serum from the infected animals. F1 hybrids displayed a delayed development of the disease when an acellular virus preparation was administered, but they were fully susceptible to the disease when syngeneic blood from infected F1 donors was transfused. Blood from donors in the prodromal stage was as effective in transmission of the disease as blood from donors with fully developed disease. This indicated that in murine lymphoproliferative disease viremia develops very early in the course of the disease. It seems that using the blood transfusion one could develop a reliable semiquantitative assay for the infectiveness of the animals suffering from LP-MB5-induced lymphoproliferative disease.
Cunningham
R K
RK
Department of Microbiology, State University of New York, Buffalo 14214.
Thacore
H R
HR
Zhou
P
P
Nakeeb
S
S
Zaleski
M B
MB
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D008232:Lymphoproliferative Disorders / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012192:Retroviridae Infections / Q000635:transmission*; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D065227:Transfusion Reaction; D014776:Virus Cultivation / Q000379:methods
D015704:CD4 Antigens; D016827:CD8 Antigens