Inter- and intra-strain genomic variation in equine herpesvirus type 1 isolates.
Restriction enzyme digests of DNA from 22 unselected isolates of EHV-1 were analysed by hybridization with cloned DNA fragments covering the genome. In addition to a small amount of inter-strain variation, heterogeneity within strains was observed, caused by loss of specific restriction endonuclease sites in the DNA of a proportion of the virus particles of any one stock. Fifteen strains demonstrated the same intra-strain variation involving loss of the BamHI L-M site which was shown to lie within coding sequence for the large subunit of ribonucleotide reductase. This particular mutation may therefore be selected for by passage in RK13 cells.
Bonass
W A
WA
Department of Microbiology, University of Leeds, U.K.
Hudson
W A
WA
Elton
D M
DM
Killington
R A
RA
Halliburton
I W
IW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D006736:Horses; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D015342:DNA Probes; D004279:DNA, Viral