[A preparative method for isolating the synaptonemal complexes from mammalian spermatocytes].
A method of isolation of synaptonemal complexes (SC) from mouse, rat and Syrian hamster spermatocytes is described. A fraction of pachytene spermatocyte nuclei was obtained by centrifugation of the testis homogenate in stepwise sucrose gradient and then lysed. The resulting chromatine was hydrolysed with DNAse II, and a fraction of isolated SCs was obtained by ultracentrifugation of the hydrolysate. The method can be applied for obtaining the SC fraction from spermatocytes sufficient for cytological, biochemical and molecular biology studies.
Dadashev
S Ia
SIa
Bogdanov
Iu F
IuF
Gorach
G G
GG
Kolomiets
O L
OL
Karpova
O I
OI
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000648:ultrastructure; D006224:Cricetinae; D008297:Male; D008540:Meiosis; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D051381:Rats; D013090:Spermatocytes / Q000648:ultrastructure*; D013573:Synaptonemal Complex
Preparativnyĭ metod vydeleniia sinaptonemnykh kompleksov iz spermatotsitov mlekopitaiushchikh.