title: Profile of Escherichia coli O157:H7 pathogen responsible for hamburger-borne outbreak of hemorrhagic colitis and hemolytic uremic syndrome in Washington.
abstract: We analyzed Escherichia coli O157:H7 isolates from stool samples of five patients who had bloody diarrhea and were infected during a large food-borne outbreak of hemorrhagic colitis in Washington state. The isolates were assessed for Shiga-like toxin profile, adherence and plasmid traits, mouse virulence, capsule, and enterohemolysin production. The profiles of the five isolates were indistinguishable from each other and similar to that of E. coli O157:H7 strain EDL933, an organism responsible for a similar hamburger-associated food poisoning episode in 1982.
authors.affiliation: Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
authors.forename: A D
authors.initials: AD
authors.lastname: O'Brien
authors.forename: A R
authors.initials: AR
authors.lastname: Melton
authors.forename: C K
authors.initials: CK
authors.lastname: Schmitt
authors.forename: M L
authors.initials: ML
authors.lastname: McKee
authors.forename: M L
authors.initials: ML
authors.lastname: Batts
authors.forename: D E
authors.initials: DE
authors.lastname: Griffin
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis; D002648:Child; D004196:Disease Outbreaks; D004926:Escherichia coli / Q000472:pathogenicity*; D005260:Female; D005516:Food Microbiology; D006471:Gastrointestinal Hemorrhage / Q000453:epidemiology* / Q000209:etiology; D006463:Hemolytic-Uremic Syndrome / Q000453:epidemiology* / Q000209:etiology; D006801:Humans; D008297:Male; D008460:Meat / Q000506:poisoning*; D051379:Mice; D022641:Shiga Toxin 2; D014774:Virulence; D014861:Washington
chemical_list: D001427:Bacterial Toxins; D022641:Shiga Toxin 2