The effect of the mode of sampling on BHV-1 detection in infected cattle by dot-blot hybridization.
The BHV-1 genome in nasal swabs and washings was detected by dot-blot hybridization using the 32P-pUR-1 probe (1.8 kb EcoRI-HindIII random fragment of BHV-1 DNA ligated into the pUC-9 plasmid) as early as on day 1 after the experimental infection of cattle. In dependence on the sampling method, differences were observed in the maximum of hybridization signals. During nasal swab analyses maximum amounts of BHV-1 differed in the individual samples (day 1-3). Hybridization signals obtained at the analysis of BHV-1 DNA nasal washings did not vary but showed a continuous maximum on day 2 after infection. Nasal washings proved to be more advantageous for detection of the BHV-1 genome by the hybridization technique.
Vilcek
S
S
Laboratory of Genetic Manipulation, Institute of Experimental Veterinary Medicine, Kosice, Slovakia.
Deliová
I
I
Strojný
L
L
Forgác
O
O
Harvan
M
M
D000818:Animals; D002417:Cattle; D004279:DNA, Viral / Q000032:analysis*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000302:isolation & purification*; D007241:Infectious Bovine Rhinotracheitis / Q000382:microbiology*; D009297:Nasal Mucosa / Q000382:microbiology; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral