title: Evaluation of reliability of pooling stool specimens from different patients and detection of Giardia lamblia antigen by microtiter enzyme-linked immunosorbent assay.
abstract: We have shown that stool samples from different patients can be pooled at a 1:2 dilution and reliably assayed for Giardia lamblia antigen by a commercial microtiter enzyme-linked immunosorbent assay (ELISA) system (LMD Laboratories, Inc., Carlsbad, Calif.). Laboratories can reduce reagent costs by pooling specimens submitted for the detection of Giardia antigen by ELISA.
authors.affiliation: Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
authors.forename: J C
authors.initials: JC
authors.lastname: McLaughlin
authors.forename: S K
authors.initials: SK
authors.lastname: Rasmussen
authors.forename: L J
authors.initials: LJ
authors.lastname: Nims
authors.forename: D A
authors.initials: DA
authors.lastname: Madar
authors.forename: C R
authors.initials: CR
authors.lastname: Yazzie
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D016829:Giardia lamblia / Q000276:immunology*; D006801:Humans
chemical_list: D000953:Antigens, Protozoan