Studies on pharmacological activation of human immunoglobulin G by chemical modification and active subfragments. X. Effect of carboxamidemethylated Fc fragment (CM-Fc) from human immunoglobulin G on delayed type hypersensitivity.
The anti-allergic activity of the carboxyamidemethylated Fc fragment (CM-Fc) from human serum immunoglobulin G (IgG) was studied using sheep red blood cell-induced delayed type hypersensitivity in mice (SRBC-DTH). CM-Fc suppressed the DTH response when administered 30 min before, or 4 h after the SRBC challenge, but not when administered 8 h or more after the challenge. The Fc fragment showed no activity. CM-Fc administration 30 min before the challenge was unable to suppress the DTH response in the cyclophosphamide (CY)-treated mice. However, adoptive transfer of splenocytes from mice treated with CM-Fc to CY-pretreated mice caused suppression of the SRBC-DTH response. These results suggest that CM-Fc suppressed the DTH response by mediating the function of CY-susceptible cells.
Mimura
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Itoh
M
M
Sugiyama
T
T
Suzuki
N
N
Nakanishi
T
T
Tsujikawa
K
K
Kohama
Y
Y
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000188:drug therapy*; D007141:Immunoglobulin Fc Fragments / Q000494:pharmacology* / Q000627:therapeutic use; D016219:Immunotherapy, Adoptive; D051379:Mice; D012756:Sheep
D007141:Immunoglobulin Fc Fragments; D003520:Cyclophosphamide