Sensitive and simple bioassay for human tumour necrosis factor-alpha.
A simple, sensitive, and specific in vitro bioassay is described for human tumour necrosis factor alpha (TNF-alpha). The system employs the U937 cell line incubated with 10 ng/ml actinomycin D for maximum sensitivity. This method allows the detection of less than 0.6 pg/ml of TNF-alpha and the log dose-response curve is linear at concentrations 0.6-250 pg/ml TNF-alpha. Highly sensitive subclones of U937 were obtained which were capable of detecting as little as 0.1 pg/ml TNF-alpha. Finally, this bioassay demonstrates specificity by discriminating between TNF-alpha and TNF-beta.
Blagosklonny
M V
MV
Clinical Pharmacology Branch, National Cancer Institute, National Institute of Health, Bethesda, MD 20892.
Neckers
L M
LM
D001681:Biological Assay; D016923:Cell Death / Q000187:drug effects; D002999:Clone Cells / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007378:Interleukins / Q000494:pharmacology; D015473:Leukemia, Promyelocytic, Acute; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D008233:Lymphotoxin-alpha / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D007378:Interleukins; D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003609:Dactinomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor