[Immunohistochemical study on the expression of perforin in tumor infiltrating lymphocytes of renal cell carcinoma].
Perforin is considered to be one of the important cytolytic factors found in the granules of killer cells. In this paper we attempted to demonstrate perforin in TIL (tumor infiltrating lymphocytes) of 55 human renal cell cancer cases and the perforin was proved by staining immunohistochemically with an anti-PFP monoclonal antibody. The result indicated that perforin was observed in 98% of the cases, while few positive cell was found in normal renal tissues. TILs were further characterized into their subsets including CD4+ T cells, CD8+ T cells, NK cells and gamma/delta T cells using antibodies specific to their surface markers. The antibodies used are anti-Lew3a, anti-Lew2a, anti-Lew19 and anti-delta chain antibody. The former two subsets were predominantly found to be infiltrating in the tumor tissue, while the latter two subsets were shown to be lesser than the former. For examination of the correlation between the presence of perforin and the characteristics of infiltrated lymphocyte subsets, double-staining of the cells was performed using anti-perforin and one of anti-surface marker antibodies, respectively. The perforin was found to be expressed on the four subsets at different extent. A stronger reactivity of the perforin was found in the CD4+ T cells and CD8+ T cells than that in the NK cells and the gamma/delta T cells. These findings suggest that perforin may play a role of effector functions in the immunological defense mechanism against cancer. To define the significance of the expression of the perforin not only on the CD8+ T cells but also on the CD4+ T cells is required for further study.
Wakumoto
Y
Y
Department of Urology, Juntendo University School of Medicine.
Sakamoto
Y
Y
D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000276:immunology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins
D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin