title: Polyamines modulate streptomycin-induced mistranslation in Escherichia coli.
abstract: The effects of intracellular levels of polyamines on both the in vivo inhibition of protein synthesis and the decrease of translation accuracy induced by streptomycin have been studied in polyamine-auxotrophic strains of Escherichia coli infected with the MS2 bacteriophage. The amount of viral coat protein formed was strongly reduced upon addition of increasing concentrations of streptomycin to polyamine-supplemented bacteria. In contrast, the antibiotic almost did not inhibit coat protein synthesis in polyamine-starved cells. The increase of mistranslation frequency elicited by streptomycin was only observed in bacteria grown with putrescine. In these cells several coat protein-satellites were detected after two-dimensional gel electrophoresis. These proteins, more basic than the normal MS2 coat protein, contain multiple substitutions of lysine for asparagine.
authors.affiliation: Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, Argentina.
authors.forename: H G
authors.initials: HG
authors.lastname: Nastri
authors.forename: I G
authors.initials: IG
authors.lastname: Fastame
authors.forename: I D
authors.initials: ID
authors.lastname: Algranati
mesh_terms: D001345:Autoradiography; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002213:Capsid / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017909:Levivirus / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D011073:Polyamines / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D013307:Streptomycin / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D011073:Polyamines; D008715:Methionine; D013307:Streptomycin