title: [Escherichia coli hemolysin: secretion kinetics and cytotoxic effect].
abstract: Escherichia coli hemolysin is one of the most important virulence determinants for uropathogenic strains. Hemolysin is cytotoxic to renal proximal tubular epithelial cells and leukocytes. It also causes the release of various mediators of inflammation and enzymes from leukocytes, thereby enhances the tissue damage to renal epithelium. The secretion kinetics of hemolysin was studied and found to be maximally secreted by an active process during logarithmic phase. The cytotoxic effect of the hemolysin was shown using Vero cells and by measuring Lactate Dehydrogenase activity in culture supernatants. The hemolytic strain showed significant cell lysis in comparison to non-hemolytic strain.
authors.affiliation: Dokuz Eylül Universitesi, Tip Fakültesi, Mikrobiyoloji Anabilim Dali.
authors.forename: H
authors.initials: H
authors.lastname: Abacioğlu
authors.forename: N
authors.initials: N
authors.lastname: Yuluğ
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000633:toxicity; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000633:toxicity; D003603:Cytotoxins / Q000096:biosynthesis* / Q000633:toxicity; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D014709:Vero Cells; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D007770:L-Lactate Dehydrogenase
vernacular_title: Escherichia coli hemolizini: salinim kinetiği ve sitotoksik etkisi.