Regional mapping of the human NSP gene to chromosome region 14q21-->q22 by fluorescence in situ hybridization analysis.
Genetic sequences of the novel NSP gene, which encodes neuroendocrine-specific proteins, were isolated from cDNA libraries constructed with mRNA isolated from human lung carcinoma cells. Hybridization analysis of a panel of human x mouse cell hybrids with an 0.8-kb NSP cDNA probe indicated that the human NSP gene is probably located on chromosome 14. Fluorescence in situ hybridization analysis of metaphase chromosomes using overlapping genomic clones of NSP as a probe localized the NSP gene to chromosome region 14q21-->q22.
Kools
P F
PF
Center for Human Genetics, University of Leuven, Belgium.
Roebroek
A J
AJ
Van de Velde
H J
HJ
Marynen
P
P
Bullerdiek
J
J
Van de Ven
W J
WJ
D000818:Animals; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods; D002883:Chromosomes, Human, Pair 14; D015342:DNA Probes; D018076:DNA, Complementary; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000302:isolation & purification; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C081781:RTN1 protein, human; C489914:Rtn1 protein, mouse