Long-term persistent infection of swine monocytes/macrophages with African swine fever virus.
Long-term persistent infection was established in 100% of pigs (n = 19) experimentally infected with African swine fever virus (ASFV). Viral DNA was detected in peripheral blood mononuclear leukocytes (PBML) at greater than 500 days postinfection by a PCR assay. Infectious virus was not, however, isolated from the same PBML samples. In cell fractionation studies of PBML, monocytes/macrophages were found to harbor viral DNA during the persistent phase of infection. This result indicates that monocytes/macrophages are persistently infected with ASFV and that ASFV-swine monocyte/macrophage interactions can result in either lytic or persistent infection.
Carrillo
C
C
Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, New York 11944.
Borca
M V
MV
Afonso
C L
CL
Onisk
D V
DV
Rock
D L
DL
D000357:African Swine Fever / Q000382:microbiology*; D000358:African Swine Fever Virus / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D004279:DNA, Viral / Q000097:blood*; D005814:Genes, Viral; D008264:Macrophages / Q000382:microbiology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013552:Swine; D013997:Time Factors
D036022:Capsid Proteins; D004279:DNA, Viral; C063745:capsid protein p72, African swine fever virus