Growth in serum-free medium improves isolation of Chlamydia pneumoniae.
Infectivity titers were determined for eight Chlamydia pneumoniae strains simultaneously grown in serum-free and serum-supplemented cell culture media. Use of serum-free medium resulted in a 10- to 50-fold increase in the susceptibility of HL cells to chlamydial infection. Comparative primary isolation of a wild-type strain also produced higher inclusion counts in a serum-free environment. Serum-free cultivation is recommended to increase the efficiency of C. pneumoniae isolation from clinical material and to permit elementary body purification without interference caused by serum components.
Maass
M
M
Institute of Medical Microbiology, Medical University of Lübeck, Germany.
Essig
A
A
Marre
R
R
Henkel
W
W
D016993:Chlamydophila pneumoniae / Q000254:growth & development* / Q000302:isolation & purification; D016895:Culture Media, Serum-Free; D006801:Humans
D016895:Culture Media, Serum-Free