Oligonucleotide probe determination of tetracycline-resistant bacteria isolated from catfish ponds.
Oligonucleotide probes for class A, B and C tetracycline resistance determinants were synthesized and tested individually and in combination for hybridization with Gram-negative isolates previously characterized by restriction fragment DNA probes. Similar hybridization patterns were observed with either probe type. Gram-negative catfish pond bacteria were characterized by these oligonucleotide probes. The most common bacteria were Plesiomonas shigelloides (47.7%), Aeromonas hydrophila (28.8%) and Citrobacter freundii (13.3%). The class A (46.4%) tetracycline resistance determinant was more prevalent than B (19.8%) and C (0.3%). Some tetracycline-resistant isolates of each species, including most A. hydrophila (62.1%) isolates, failed to hybridize with any of the probes.
DePaola
A
A
Division of Seafood Research, Food and Drug Administration, Dauphin Island, Alabama 36528.
Hill
W E
WE
Harrell
F M
FM
D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002397:Catfishes; D004269:DNA, Bacterial / Q000235:genetics; D005618:Fresh Water; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D013753:Tetracycline Resistance / Q000235:genetics*; D014871:Water Microbiology
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes