title: Reduction of immunoreactivity of bovine serum albumin conjugated with comb-shaped polyethylene glycol derivatives.
abstract: Bovine serum albumin (BSA) was chemically modified with two types of comb-shaped copolymers of polyethylene glycol derivative and maleic anhydride, one with the molecular weight of 13,000 (activated PM13) and the other with 100,000 (activated PM100), to form PM13- and PM100-BSA. The immunoreactivity of BSA was markedly reduced by coupling with each modifier and was completely lost when 30% or 20% of amino groups in BSA were modified with activated PM13 or PM100, respectively. The esterase activity of PM13- and PM100-BSA without immunoreactivity were retained 63% and 93% of non-modified one, respectively. These results were discussed with those of modified-asparaginases(1).
authors.affiliation: Department of Materials Science and Technology, Toin University of Yokohama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtake
authors.forename: A
authors.initials: A
authors.lastname: Matsushima
authors.forename: M
authors.initials: M
authors.lastname: Hiroto
authors.forename: Y
authors.initials: Y
authors.lastname: Kodera
authors.forename: Y
authors.initials: Y
authors.lastname: Inada
mesh_terms: D001215:Asparaginase / Q000737:chemistry / Q000276:immunology; D008299:Maleic Anhydrides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011233:Precipitin Tests; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology*
chemical_list: D008299:Maleic Anhydrides; C054144:polyoxyethylene allylmethyl diether-maleic anhydride copolymer; C084496:polyoxyethylene (2-methyl-2-propenyl) methyl diether-maleic anhydride copolymer; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D001215:Asparaginase