Increase of fucosylated serum cholinesterase in relation to high risk groups for hepatocellular carcinomas.
Serum cholinesterase (ChE) (E.C. 3.1.1.8) is a glycoprotein which has 36 potential sites of asparagine-N-linked sugar chains. The structures of oligosaccharides released from ChE on hydrazinolysis were studied by serial lectin affinity column chromatography, exoglycosidase digestion, and methylation analysis. Seventy-three % of the sugar chains occurred as biantennary oligosaccharides and the remainder as C-2 and C-2,4/C-2,6 branched tri- and tetraantennary oligosaccharides. Several percentages of the Lewis X antigenic determinant and fucosylated mannose core were linked to them, and their sialic acid residues were linked to nonreducing terminal galactose residues at the C-3 and C-6 positions. Aleuria aurantia lectin-reactive ChE with the Lewis X antigenic determinant increased in hepatocellular carcinomas and liver cirrhosis compared with chronic hepatitis; on the other hand, Aleuria aurantia lectin-reactive ChE did not change significantly after transcatheter arterial embolization and was not related to the serum levels of alpha-fetoprotein and carcinoembryonic antigen in patients with hepatocellular carcinomas. Accordingly, the analysis of Aleuria aurantia lectin-reactive ChE is clinically useful for differentiating liver cirrhosis from chronic hepatitis and to identify high risk groups for hepatocellular carcinomas, i.e., cirrhotic patients in Child's A grade.
Ohkura
T
T
Department of Biochemistry, Sasaki Institute, Tokyo, Japan.
Hada
T
T
Higashino
K
K
Ohue
T
T
Kochibe
N
N
Koide
N
N
Yamashita
K
K
D015415:Biomarkers / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000201:enzymology*; D002802:Cholinesterases / Q000097:blood* / Q000737:chemistry; D003937:Diagnosis, Differential; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000201:enzymology*; D008113:Liver Neoplasms / Q000175:diagnosis / Q000201:enzymology*; D009363:Neoplasm Proteins / Q000097:blood* / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis; D011230:Precancerous Conditions / Q000175:diagnosis / Q000201:enzymology*; D012307:Risk Factors
D015415:Biomarkers; D037102:Lectins; D009363:Neoplasm Proteins; D009844:Oligosaccharides; D002802:Cholinesterases