Identification of DNA binding proteins in vaccinia virus by DNA-protein crosslinking.
DNA binding proteins of vaccinia virus (VV) virions, strain LIVP, have been studied by their covalent crosslinking to DNA, using two-dimensional gel retardation electrophoresis of crosslinked DNA and proteins as well as the 'protein image' hybridization assay. Five proteins with molecular masses of 16, 25, 27, 41 and 54 kDa, respectively, associated with all analysed DNA sequences, including early and late genes and their promoters, have been identified.
Chkheidze
A N
AN
W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Prikhod'ko
G G
GG
Karpov
V L
VL
Vassilenko
S K
SK
Mirzabekov
A D
AD
D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D014616:Vaccinia virus / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry / Q000302:isolation & purification*
D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D014764:Viral Proteins