title: Transferrin-binding proteins isolated from Neisseria meningitidis elicit protective and bactericidal antibodies in laboratory animals.
abstract: Transferrin-binding proteins (Tbps) were affinity-isolated from group B Neisseria meningitidis strain B16B6 and used to raise specific antisera. Administration of the antisera to mice loaded with human transferrin before bacterial challenge significantly protected the animals from death. In active immunization studies, mice received three 25 micrograms injections of purified Tbps over a period of 28 days, 7 days after which they were challenged with N. meningitidis. The survival rate in immunized mice was much higher than in control groups. In both active and passive immunization experiments mice were protected against at least 100 LD50. A specific Tbp antiserum was highly bactericidal against the parent strain and against approximately half of the strains tested.
authors.affiliation: Pasteur Mérieux Sérums et Vaccins, Marcy l'Etoile, France.
authors.forename: B
authors.initials: B
authors.lastname: Danve
authors.forename: L
authors.initials: L
authors.lastname: Lissolo
authors.forename: M
authors.initials: M
authors.lastname: Mignon
authors.forename: P
authors.initials: P
authors.lastname: Dumas
authors.forename: S
authors.initials: S
authors.lastname: Colombani
authors.forename: A B
authors.initials: AB
authors.lastname: Schryvers
authors.forename: M J
authors.initials: MJ
authors.lastname: Quentin-Millet
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology / Q000494:pharmacology; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology*; D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D005260:Female; D007106:Immune Sera; D007116:Immunization, Passive; D033862:Iron-Binding Proteins; D008297:Male; D008589:Meningococcal Infections / Q000517:prevention & control; D051379:Mice; D008815:Mice, Inbred Strains; D009345:Neisseria meningitidis / Q000276:immunology*; D011817:Rabbits; D033863:Transferrin-Binding Proteins; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D007106:Immune Sera; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins