[Comparative studies of the detection of antibodies against BHV-5 using the enzyme-linked immunosorbent assay, serum neutralization test, cell assay and immunofluorescence test].
A comparison of the analyses of 490 serum samples from various flocks of sheep for the detection of BHV-5 antibodies using ELISA, serum neutralisation test, cell assay and immunofluorescence assay showed nearly identical results regarding the distinction between positive and negative serum samples. The percentage of serum samples with antibodies against BHV-5 antigen differed from test to test: ELISA 268 (54.7%), cell assay 323 (65.8%), immunofluorescence assay 222 (46.6%) and serum neutralisation test 221 (45.1%). With ELISA, cell assay and immunofluorescence assay the experiment can be carried out more simply and rapidly than with serum neutralisation test.
Lindner
A
A
Institut für Mikrobiologie und Tierseuchenlehre, Veterinärmedizinischen Fakultät, Universität Leipzig.
Ambrosius
H
H
Liebermann
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary; D009500:Neutralization Tests; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis
D000914:Antibodies, Viral
Vergleichende Untersuchungen zum Nachweis von Antikörpern gegen BHV-5 mit dem Enzym-linked Immunosorbent Assay, Serumneutralisationstest, Zellassay und Immunfluoreszenztest.