Comparison of serological tests for the diagnosis of Chlamydia psittaci infection of sheep.
An enzyme-linked immunosorbent assay (ELISA) was developed and compared with the complement fixation test (CFT) and the indirect immunofluorescence antibody test (IIFAT) for the detection of antibodies to C. psittaci in sheep. Field sera and experimental sera from aborting ewes, subclinically infected sheep and vaccinated sheep were used. The ELISA and IIFAT were much more sensitive than the CFT. Agreement between the CFT and ELISA varied between 50% and 98%, while agreement between the IIFAT and ELISA varied between 70.5% and 94.3%. The specificity of IIFAT and ELISA with regard to the identification of animals which may be a source of ovine abortion strains of C. psittaci was critically assessed, using antigen preparations derived from an abortion isolate and from a non-abortion isolate of C. psittaci. Indirect immunofluorescence was shown to be the more discerning test.
Markey
B K
BK
Faculty of Veterinary Medicine, Ballsbridge, Dublin, Ireland.
McNulty
M S
MS
Todd
D
D
D000034:Abortion, Veterinary / Q000175:diagnosis*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002691:Chlamydophila psittaci / Q000276:immunology*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D011247:Pregnancy; D009956:Psittacosis / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial