title: Evaluation of HLA in detection of non-parentage among known false trios.
abstract: This paternity study was performed with trios in which the putative father was not the biological father (NBF), in order to evaluate the effectiveness of HLA-A and -B phenotyping for routine testing. All 372 generated trios had ABO, Rh, MNS, Kell, Duffy, Kidd and HLA (A and B) systems tested. HLA-A and -B phenotyping directly excluded 81.73% of NBF. Red blood cell markers excluded 8.82% of NBF missed by HLA; only 0.81% of NBF were not identified by the markers used. Each laboratory engaged in disputed paternity testing should evaluate its actual performance in detecting non-biological fathers and its exclusion rates in real trios. These data together can be used as a guide of mother's reliability.
authors.affiliation: Department of Legal Medicine, Faculty of Medicine, University of São Paulo, Brazil.
authors.forename: N N
authors.initials: NN
authors.lastname: Salaru
mesh_terms: D000328:Adult; D002648:Child; D005260:Female; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D008297:Male; D010334:Paternity; D010641:Phenotype; D012680:Sensitivity and Specificity
chemical_list: D006680:HLA Antigens