Identification of a seven transmembrane helix receptor for corticotropin-releasing factor and sauvagine in mammalian brain.
We have identified and characterized cDNAs encoding a novel receptor that is a member of a distinct class of seven transmembrane helix, Gs-coupled receptors. This receptor mediates ligand-dependent stimulation of intracellular cAMP levels in response to physiologic concentrations of corticotropin-releasing factor (CRF) and to the related frog skin peptide, sauvagine. The pattern of CRF receptor mRNA expression in the brain, pituitary gland, and other organs corresponds precisely to that predicted for the classic CRF receptor, suggesting that this receptor serves to mediate the known biological effects of CRF on behavior, stress, and homeostasis. Alternative splicing events generate a second, relatively abundant gene product expressed in a distinct ontogenic pattern. These findings serve to identify the receptor for an important neuropeptide.
Chang
C P
CP
Howard Hughes Medical Institute, Department and School of Medicine, University of California, San Diego, La Jolla 92093-0648.
Pearse
R V
RV
O'Connell
S
S
Rosenfeld
M G
MG
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D029845:Amphibian Proteins; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002531:Cerebellum / Q000378:metabolism*; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney; D008969:Molecular Sequence Data; D036361:Peptide Hormones; D010455:Peptides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D015347:RNA Probes; D051381:Rats; D018019:Receptors, Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014665:Vasodilator Agents / Q000378:metabolism*
D029845:Amphibian Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D036361:Peptide Hormones; D010455:Peptides; D015347:RNA Probes; D018019:Receptors, Corticotropin-Releasing Hormone; D011994:Recombinant Proteins; D014665:Vasodilator Agents; C025727:sauvagine; D000242:Cyclic AMP