title: Recombinant cytokines as immunological adjuvants.
abstract: This paper describes the bacterial expression and purification of bioactive recombinant ovine interleukin-2 (rovIL-2), interleukin-1 alpha (rovIL-1 alpha) and tumour necrosis factor alpha. These purified proteins had specific activities in appropriate bioassays of 1 x 10(7) 1 x 10(7) and 1 x 10(5) U/mg, respectively. Recombinant ovIL-1 alpha was assessed as an immunological adjuvant for the sheep response to the model protein avidin. When delivered either intradermally or intramuscularly in conjunction with avidin in aluminium hydroxide the rovIL-1 alpha significantly enhanced the secondary humoral response. Doses of 1, 10 or 100 micrograms per sheep enhanced the humoral response to a similar extent. Recombinant ovIL-1 beta had similar adjuvant activity in that it was demonstrated to significantly enhance the sheep humoral response to an experimental H. contortus antigen. This increase in specific antibody, however, did not correlate with enhanced protection against infection with third stage H. contortus larvae. In addition incorporation of rovIL-1 beta into the formulation was shown not to alter the isotype profile of H. contortus antigen specific antibody.
authors.affiliation: Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: A D
authors.initials: AD
authors.lastname: Nash
authors.forename: S A
authors.initials: SA
authors.lastname: Lofthouse
authors.forename: G J
authors.initials: GJ
authors.lastname: Barcham
authors.forename: H J
authors.initials: HJ
authors.lastname: Jacobs
authors.forename: K
authors.initials: K
authors.lastname: Ashman
authors.forename: E N
authors.initials: EN
authors.lastname: Meeusen
authors.forename: M R
authors.initials: MR
authors.lastname: Brandon
authors.forename: A E
authors.initials: AE
authors.lastname: Andrews
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D016207:Cytokines / Q000276:immunology* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D006188:Haemonchiasis / Q000276:immunology / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology; D007375:Interleukin-1 / Q000096:biosynthesis; D010641:Phenotype; D016052:Protozoan Vaccines / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D007375:Interleukin-1; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha