Crystallization and preliminary X-ray analysis of (1,3)- and (1,3;1,4)-beta-D-glucanases from germinating barley.
(1,3)-beta-D-Glucanase isoenzyme GII and (1,3;1,4)-beta-D-glucanase isoenzyme EII from barley have been crystallized by the hanging drop method in the presence of ammonium sulphate. The crystals of the (1,3)-beta-D-glucanase, which diffract to about 1.8 A resolution, belong to the trigonal space group P3(1)2(1)2 (or P3(2)2(1)2) with cell constants a = b = 86.9 A, c = 156.0 A and contain two molecules in the asymmetric unit. The crystals of the (1,3;1,4)-beta-D-glucanase which diffract to better than 1.8 A resolution, belong to the tetragonal space group P4(3)2(1)2 (or P4(1)2(1)2) with cell constants a = b = 87.4 A, c = 109.5 A and contain one molecule in the asymmetric unit.
Chen
L
L
Department of Biochemistry, La Trobe University, Bundoora, Victoria, Australia.
Garrett
T J
TJ
Varghese
J N
JN
Fincher
G B
GB
Høj
P B
PB
D003460:Crystallization; D018360:Crystallography, X-Ray; D043326:Glucan 1,3-beta-Glucosidase; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D001467:Hordeum / Q000201:enzymology*; D007527:Isoenzymes / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D001617:beta-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification
D007527:Isoenzymes; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase; C028020:licheninase