title: Interfacial reactions of osteoblasts to dental and implant materials.
abstract: Scanning electron microscopy was used to study the response of osteoblasts to various surfaces including ceramics and glasses as well as steel and titanium. Hydroxylapatite, tricalcium phosphate, bioglass, steel, and titanium supported cell adhesion. However, the toxic effects of the In-Ceram (Vita, Bad Säckingen, Germany), and feldspar ceramic and glaze were severe enough to cause verrucous necrosis that was identifiable after 2 days of culture. After 10 days in culture only the peripheral portions of these specimens were still occupied by cells; the cells in the central portion of the circular specimens had succumbed to necrosis. It was concluded that scanning electron microscopy is useful in identifying the response of cells to materials. Pathologic changes are not recognizable if they are limited to the internal structure of the cell, but readily discernible when they impinge on the morphologic integrity of the cell surface.
authors.affiliation: University Hospital Freiburg, Department of Oral and Maxillofacial Surgery, Germany.
authors.forename: F B
authors.initials: FB
authors.lastname: Bagambisa
authors.forename: H F
authors.initials: HF
authors.lastname: Kappert
authors.forename: W
authors.initials: W
authors.lastname: Schilli
mesh_terms: D000537:Aluminum Oxide / Q000633:toxicity*; D000538:Aluminum Silicates / Q000633:toxicity*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death; D002460:Cell Line / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D003764:Dental Materials / Q000633:toxicity; D003776:Dental Porcelain / Q000633:toxicity*; D005898:Glass; D007811:Lanthanum / Q000633:toxicity; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009336:Necrosis; D010006:Osteoblasts / Q000187:drug effects* / Q000648:ultrastructure; D017680:Potassium Compounds / Q000633:toxicity*; D019736:Prostheses and Implants / Q000009:adverse effects*; D012822:Silicon Dioxide / Q000633:toxicity; D013499:Surface Properties / Q000187:drug effects
chemical_list: D000538:Aluminum Silicates; D003764:Dental Materials; D017680:Potassium Compounds; D003776:Dental Porcelain; C016447:feldspar; C070870:In-Ceram; C058236:Vitadur N; D007811:Lanthanum; D012822:Silicon Dioxide; D000537:Aluminum Oxide