title: Biosensor for direct determination of glucose and lactate in undiluted biological fluids.
abstract: This paper describes the implementation of a bienzyme sensor for the direct determination of glucose and lactate in undiluted biological samples. The biosensor exploits the competitive action versus the substrate itself by two different enzymes immobilized into a sandwich of two different membranes. In this way the quantity of substrate reaching the indicating electrode is reduced and this determines an extension of the linearity range.
authors.affiliation: Dipartimento di Chimica, Universitá La Sapienza, Roma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Campanella
authors.forename: M
authors.initials: M
authors.lastname: Tomassetti
authors.forename: F
authors.initials: F
authors.lastname: Mazzei
mesh_terms: D015374:Biosensing Techniques; D001826:Body Fluids / Q000737:chemistry*; D002138:Calibration; D005947:Glucose / Q000032:analysis*; D006801:Humans; D007773:Lactates / Q000032:analysis*; D019344:Lactic Acid
chemical_list: D007773:Lactates; D019344:Lactic Acid; D005947:Glucose