Phospholipid impregnation of abdominal rubber drains: resistance to bacterial adherence but no effect on drain-induced bacterial translocation.
In order to evaluate the effect of surface modification of biomaterials on bacterial adherence and bacterial translocation after intraperitoneal biomaterial implantation, phosphatidylcholine- or phosphatidylinositol-impregnated rubber drain pieces, which had been intraperitoneally implanted in the rat for 2 and 7 days, or unimplanted, were incubated in vitro with 3H-labelled Escherichia coli and Enterobacter cloacae. As compared with unimpregnated pieces, the adherence of bacteria significantly decreased to phosphatidylcholine- and phosphatidylinositol-impregnated rubber drain pieces that were either unimplanted or implanted for 2 days, but not for 7 days. The supplementation of albumin in the medium reduced the adherence of bacteria to the unimplanted, unimpregnated drain pieces, but did not further decrease adherence of bacteria to the unimplanted, phospholipid-impregnated brain pieces. Bacterial growth was inhibited after incubation in nutrient broth supplemented with phospholipids. The incidence of enteric bacterial translocation induced by intraperitoneal drain implantation did not differ between phospholipid-impregnated and unimpregnated drain pieces. Scanning electron microscopy revealed a large amount of biofilm and fibrous deposition on the surface of the implanted, phospholipid-impregnated rubber drain pieces. Thus, phospholipid impregnation of rubber drains reduces bacterial adherence and inhibits bacterial growth, without influencing the incidence of bacterial translocation.
Guo
W
W
Department of Surgery, Lund University, Sweden.
Andersson
R
R
Odselius
R
R
Ljungh
A
A
Wadström
T
T
Bengmark
S
S
D000005:Abdomen / Q000382:microbiology*; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002465:Cell Movement; D004322:Drainage / Q000295:instrumentation*; D016972:Enterobacter cloacae / Q000502:physiology* / Q000648:ultrastructure; D004866:Equipment Contamination; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000494:pharmacology*; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D012408:Rubber
D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D012408:Rubber