Intrathecal catheterization alone reduces autotomy after sciatic cryoneurolysis in the rat.
There is substantial evidence that sciatic cryoneurolysis (SCN, freeze lesion of the sciatic nerve) is a neuropathic pain model in the rat. During characterization of this model, SCN was performed 4 days after either a sham operation or the insertion of an indwelling intrathecal catheter preparatory to selective spinal drug administration. Body weight and autotomy scores were recorded for the next 22 days until sacrifice. The catheter group experienced significant weight loss (7.5%) by 4 days but rapidly regained to parity with the sham group. Autotomy scores and the frequency of severe autotomy (score > 3) were less at day 22 in the catheter group as compared with the sham-control group (p < 0.005, p < 0.03, respectively). Intrathecal catheterization itself effects the degree of behavioral response to neurogenic pain and thus, should be controlled for in studies using nociceptive animal models.
Serpell
M G
MG
Department of Anesthesiology, Dartmouth-Hitchcock Medical Center, Lebanon, N.H. 03756.
DeLeo
J A
JA
Coombs
D W
DW
Colburn
R W
RW
Twitchell
B B
BB
Willenbring
S
S
Fromm
C
C
D000818:Animals; D016477:Artifacts; D002404:Catheterization / Q000379:methods*; D002408:Catheters, Indwelling; D004195:Disease Models, Animal; D005615:Freezing; D007263:Infusions, Parenteral / Q000379:methods; D008297:Male; D010146:Pain / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012107:Research Design; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology*