[Hydrolysis of DNA-duplexes, containing 5-fluorodeoxycytidine by restriction endonucleases].
Cleavage by restriction endonucleases MvaI kappa EcoRII of DNA duplexes, in which the internal deoxycytidine in one of the strands of the recognition site is substituted for 5-fluorodeoxycytidine, has been studied. It has been found that the modified strands of these duplexes are scissed by endonuclease MvaI with a greater efficiency than the intact ones. Endonuclease EcoRII does not practically cleave such substrate analogs. The UV absorbance spectra of 5-fluorodeoxycytidine and the 14-member oligodeoxyribonucleotide with modification in the middle of the strand have been measured. A hypothesis has been put forward about the structural features of 5-fluorodeoxycytidine-containing DNA duplexes.
Sheflian
G Ia
GIa
Kubareva
E A
EA
Gromova
E S
ES
Shabarova
Z A
ZA
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008969:Molecular Sequence Data; D013056:Spectrophotometry, Ultraviolet
D003841:Deoxycytidine; D004247:DNA; C029528:DNA modification methylase EcoRII; D015257:DNA-Cytosine Methylases; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C007746:5-fluoro-2'-deoxycytidine
Issledovanie gidroliza DNK-dupleksov, soderzhashchikh 5-ftordezoksitsitidin, éndonukleazami restriktsii.