Development of behavioral tolerance to delta 9-THC without alteration of cannabinoid receptor binding or mRNA levels in whole brain.
The effect of repetitive administration of delta-9-tetrahydrocannabinol (delta 9-THC) in mice on behavioral and biochemical tolerance was determined in this study. Mice were injected twice daily with 10 mg/kg delta 9-THC for 6.5 days. On day 8, spontaneous activity was assessed or whole-brain homogenates were prepared for the cannabinoid receptor binding and mRNA studies. Although a twenty-sevenfold tolerance to delta 9-THC was observed in the behavioral assay, there was no significant alteration in receptor binding or mRNA levels.
Abood
M E
ME
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Sauss
C
C
Fan
F
F
Tilton
C L
CL
Martin
B R
BR
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000187:drug effects*; D003511:Cyclohexanols / Q000494:pharmacology; D015342:DNA Probes; D013759:Dronabinol / Q000494:pharmacology*; D004361:Drug Tolerance; D004591:Electrophoresis, Polyacrylamide Gel; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000096:biosynthesis*; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug / Q000187:drug effects* / Q000378:metabolism
D003511:Cyclohexanols; D015342:DNA Probes; D012333:RNA, Messenger; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug; D013759:Dronabinol; C054649:3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanol