Phosphatidylserine specific binding protein in rat brain: purification and characterization.
A calcium-independent phosphatidylserine specific binding protein detected on liposome blotting analysis was purified from rat brain and revealed to be identical to myristoylated, alanine-rich C kinase substrate (MARCKS). MARCKS specifically binds to phosphatidylserine but not phosphatidylcholine. The binding of MARCKS to phosphatidylserine was abolished on protein kinase C-dependent phosphorylation. Since bacterially expressed MARCKS also specifically binds to phosphatidylserine, myristoylation of the N-terminal glycine seems not to be essential for the binding of MARCKS to phosphatidylserine. These data suggest that phosphatidylserine is a membranous target molecule of MARCKS.
Nakaoka
T
T
Glyco Molecular Biology, Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Saitama.
Kojima
N
N
Hamamoto
T
T
Kurosawa
N
N
Lee
Y C
YC
Kawasaki
H
H
Suzuki
K
K
Tsuji
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002793:Cholic Acids; D047908:Intracellular Signaling Peptides and Proteins; D008081:Liposomes; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D010718:Phosphatidylserines / Q000378:metabolism*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
D002793:Cholic Acids; D047908:Intracellular Signaling Peptides and Proteins; D008081:Liposomes; C000619712:Marcks protein, rat; D008565:Membrane Proteins; D010718:Phosphatidylserines; D011506:Proteins; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate