Preliminary crystallographic study of a complex between an Fab of a monoclonal feline peritonitis virus neutralizing antibody and its anti-idiotypic Fab.
A complex between an Fab fragment of an E2 specific feline infectious peritonitis virus neutralizing antibody 730.1.4 and Fab fragment from anti-idiotypic monoclonal antibody 409.5.3, was crystallized from ammonium sulfate using vapor diffusion methods. The complex crystals diffract to about 2.9 A resolution and are of orthorhombic space group P2(1)22(1) with a = 75.2 A, b = 80.6 A and c = 187.6 A. There are two Fab molecules, or one idiotype-anti-idiotype complex, comprising the asymmetric unit. The long 187.6 A c axis suggests that the long axis of the complex might lie along this direction. Although small and radiation-sensitive, crystals are suitable for three-dimensional structural analysis.
Ban
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Department of Biochemistry, University of California at Riverside 92521.
Escobar
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Day
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Greenwood
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McPherson
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000378:metabolism*; D000914:Antibodies, Viral / Q000737:chemistry / Q000378:metabolism*; D002415:Cats; D016765:Coronavirus, Feline / Q000276:immunology*; D018360:Crystallography, X-Ray; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D014961:X-Ray Diffraction
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments