Presence of gene expression of vitamin D receptor and 24-hydroxylase in OK cells.
We investigated the gene expression of 1,25-dihydroxy-vitamin D3 (1,25(OH)2D3) receptors (VDR) and 25-hydroxy-vitamin D3-24-hydroxylase (24-hydroxylase) in opossum kidney (OK) cells. Reverse transcription-polymerase chain reaction demonstrated the presence of VDR gene expression in OK cells. Northern hybridization, using rat VDR cDNA, also demonstrated a 4.4 kbp VDR mRNA expression which was affected by serum but not by 1,25(OH)2D3 and/or parathyroid hormone stimulation. Stimulation with 1,25(OH)2D3 induced 24-hydroxylase mRNA expression which peaked at 6 h. This study clearly demonstrates for the first time the presence of VDR and 24-hydroxylase gene expression in OK cells, suggesting that OK cells are a useful model for investigating the genetic action mechanisms of 1,25(OH)2D3.
Ishimura
E
E
Second Department of Internal Medicine, Osaka City University Medical School, Japan.
Shoji
S
S
Koyama
H
H
Inaba
M
M
Nishizawa
Y
Y
Morii
H
H
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002117:Calcitriol / Q000494:pharmacology; D002460:Cell Line; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D007668:Kidney; D007700:Kinetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009893:Opossums; D010281:Parathyroid Hormone / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics*; D013250:Steroid Hydroxylases / Q000235:genetics*; D065668:Vitamin D3 24-Hydroxylase
D010281:Parathyroid Hormone; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D065668:Vitamin D3 24-Hydroxylase; D002117:Calcitriol