mRNA-ribosome interactions.
Synthetic mRNA analogues were constructed with sequences related to the Cro-protein mRNA from lambda-phage and prepared by T7 transcription. Each mRNA contained several thiouridine (thio-U) residues. The regions upstream from the AUG initiator codon of the mRNA were the same in all the messages, whereas in the downstream part the thio-U residues were placed in selected positions. These positions covered the region from +4 to +16 (A in the initiator AUG codon being defined as +1). After binding to the ribosome in the presence of initiator tRNA the thio-U residues were activated by u.v. irradiation and the resulting sites of cross-linking to 16 S rRNA and ribosomal proteins were analysed. Cross-links to several ribosomal proteins were identified in different types of complex. Changes in the conformation of the small ribosomal subunit in different initiation and elongation complexes are discussed.
Dokudovskaya
S S
SS
Chemistry Faculty, Moscow State University, Russia.
Dontsova
O A
OA
Bogdanova
S L
SL
Bogdanov
A A
AA
Brimacombe
R
R
D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000138:chemical synthesis / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins
D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S; D012097:Repressor Proteins; D012269:Ribosomal Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins