The carrot secreted glycoprotein gene EP1 is expressed in the epidermis and has sequence homology to Brassica S-locus glycoproteins.
Non-embryogenic carrot suspension cells secrete the EP1 glycoprotein. A cDNA clone encoding EP1 was isolated and sequenced. The EP1 sequence revealed a region of homology with Brassica S-locus glycoprotein genes, an Arabidopsis S-like gene and putative S-like receptor protein kinases from maize and Arabidopsis. EP1 gene expression, analysed by in situ mRNA localization, was detected in cells located at the surface of the seedling: in the epidermis of the root, the hypocotyl and the cotyledons, in the root cap, and in a crescent of cells in the apical dome of the shoot. In developing seeds, expression was most pronounced in both the inner and outer integument epidermis.
van Engelen
F A
FA
Department of Molecular Biology, Agricultural University Wageningen, The Netherlands.
Hartog
M V
MV
Thomas
T L
TL
Taylor
B
B
Sturm
A
A
van Kammen
A
A
de Vries
S C
SC
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014675:Vegetables / Q000235:genetics*
D018076:DNA, Complementary; C085181:EP1 protein, Daucus carota; D006023:Glycoproteins; D010940:Plant Proteins; D012333:RNA, Messenger; C064156:self-incompatibility locus specific glycoprotein, Brassica