Isolation and characterization of the human genomic locus coding for the putative metastasis control gene nm23-H1.
Nm23-H1 gene expression is inversely correlated with tumor metastatic potential in certain tumors, including melanomas, breast carcinomas, and hepatocellular carcinomas. Using nm23-H1 c-DNA primer and genomic polymerase chain reaction (PCR) amplification, we purified three PCR fragments (one of 4kb and two of 2 kb) covering the whole human genomic locus of the gene (8.460bp). We recombined the PCR products into pUC18 and produced a restriction map to perform subcloning. Complete sequencing of genomic PCR fragments, including the whole coding region of nm23-H1, revealed that the gene consists of five exons and four introns spanning 8.5kb. A sequence homology analysis between human nm23-H1 and the homolog gene of the rat (NDP-K beta) shows that exon-intron boundaries are well conserved between these two species.
Dooley
S
S
Institut für Humangenetik, Universität des Saarlandes, Homburg-Saar, Germany.
Seib
T
T
Engel
M
M
Theisinger
B
B
Janz
H
H
Piontek
K
K
Zang
K D
KD
Welter
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005091:Exons; D005260:Female; D015870:Gene Expression; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009362:Neoplasm Metastasis / Q000235:genetics*; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics / Q000302:isolation & purification*; D010920:Placenta / Q000737:chemistry; D016133:Polymerase Chain Reaction; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification*
D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins