Influence of bromocriptine administration to mothers on the development of pup thymocyte and splenocyte subsets and on mitogen-induced proliferation in the mouse.
The influence of prolactin (PRL) on the development of the immune system in the mouse was studied by injecting mothers with bromocriptine (CB-154) to produce hypoprolactinemic milk. Alterations in pup thymocyte and splenocyte cell subsets were observed to graded doses of CB-154 administered to mothers. There was an increase in the relative percentages of neonate thymic CD4 and CD8 cells at 5 days of age when mothers were injected with 100 micrograms of CB-154 2 x daily from day 1 to 5 of lactation, however, there was no alteration in absolute thymic subset cell numbers. The relative percentage of pup spleen CD4, CD8 and B cells were increased when mothers were administered 50 or 100 micrograms of CB-154 and the 50 micrograms dose resulted in a significant increase in the absolute number of CD4 cells while the 100 micrograms dose induced a significant decrease in the three splenic cell subsets examined. Graded doses of CB-154 administered to mothers resulted in decreases in the PRL concentration of stomach milk as measured by the Nb2 cell proliferation assay. The serum PRL level of the pups, however, was not altered by any dose of CB-154 injected to the mothers. The administration of PRL to pups nursing mothers given the 100 micrograms dose of CB-154 did not alter the pup thymocyte and splenocyte subset population from that of litter-mate controls. The administration of mouse PRL and mouse growth hormone antisera to pups nursing saline-injected mothers did not alter thymocyte and splenocyte subsets from that of saline-injected litter mate controls. The proliferation of neonatal thymocytes by Con-A stimulation was not altered by CB-154 injection to mothers and PRL administration to pups. However, since the percentage of thymic CD4 and CD8 cells in the thymus was increased 2 to 3 fold, the apparent lack of effect was in fact a decrease in the responsiveness of the thymocytes. Con-A stimulation of neonatal splenocytes resulted in a significant increase in proliferation for mothers administered CB-154 in keeping with the increase relative percentage of CD4 and CD8 cells observed. Prolactin administration to the pups did not alter the response. LPS stimulation of neonatal splenocytes increased the proliferation of B cells taken from pup nursing mothers administered CB-154 and PRL administration appeared to partially block this proliferation.(ABSTRACT TRUNCATED AT 400 WORDS)
Gala
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RR
Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201.
Shevach
E M
EM
D000818:Animals; D000833:Animals, Suckling / Q000254:growth & development* / Q000276:immunology; D001971:Bromocriptine / Q000494:pharmacology*; D016516:CD4-CD8 Ratio / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D013006:Growth Hormone / Q000276:immunology; D007774:Lactation / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D016131:Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology*; D011247:Pregnancy; D011388:Prolactin / Q000097:blood / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000254:growth & development* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000254:growth & development* / Q000276:immunology
D008934:Mitogens; D001971:Bromocriptine; D011388:Prolactin; D013006:Growth Hormone