Megakaryocyte colony stimulating activity in allogenic bone marrow recipients prepared with busulfan and cyclophosphamide.
Increased megakaryocyte colony stimulating activity (MK-CSA) has been reported after total body irradiation (TBI) for bone marrow transplant (BMT). We studied the effect of a busulfan (Bu) and cyclophosphamide (Cy) marrow transplant conditioning regimen, without radiation, on MK-CSA production. Initial screening of MK-CSA was done on previously collected and banked sera from 14 BMT patients. MK-CSA was expressed as the ability to stimulate growth of megakaryocyte progenitors (CFU-MK) in standard plasma clot cultures. In the initial samples, MK-CSA peaked at day 7. This preliminary data led to a prospective study of MK-CSA and clinical parameters in seven allogeneic recipients. MK-CSA activity increased from day -7 pre-transplant (2.9 +/- 1.7 CFU-MK/10(5) NATD, mean +/- SD) to day 0 (10.3 +/- 4.7 CFU-MK) and peaked by day 9 post-transplant (20.6 +/- 6.4 CFU-MK). MK-CSA activity decreased in all seven patients by day 21 at which time five of seven patients studied had recovery of platelet counts to greater than 100 x 10(9)/l. MK-CSA activity rose rapidly in both groups of sera after the initiation of this non-irradiation, BMT preparative regimen. High MK-CSA levels, early after transplant, may contribute to the rapid platelet recovery in some patients.
Olson
T A
TA
Department of Pediatrics, Columbus Children's Hospital, Ohio State University.
Kapoor
N
N
Wing
C
C
Tutschka
P
P
D000328:Adult; D016026:Bone Marrow Transplantation / Q000502:physiology*; D002066:Busulfan / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000187:drug effects*; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D058851:GPI-Linked Proteins; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000473:pathology; D008562:Membrane Glycoproteins; D000090204:Mesothelin; D010976:Platelet Count; D011184:Postoperative Period; D011446:Prospective Studies; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects*; D012189:Retrospective Studies
D003115:Colony-Stimulating Factors; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D011506:Proteins; D003520:Cyclophosphamide; D002066:Busulfan; D000090204:Mesothelin