Chromatin structure: mapping proteins associated with genomic DNA using crosslinking.
The structure and structural dynamics of chromatin and nucleosomes in transcription and replication were studied by DNA-protein crosslinking. A general strategy has been designed and a number of methods have been developed to map proteins bound to the genomic DNA, to sequence the arrangement of such proteins along DNA in multi-protein complexes, and to identify interacting nucleotides and amino acid residues. Perspectives for large-scale mapping of proteins associated with genomic DNA are discussed.
Mirzabekov
A D
AD
Engelhardt Institute of Molecular Biology, Moscow, Russia.
D000595:Amino Acid Sequence; D002843:Chromatin / Q000737:chemistry*; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data
D002843:Chromatin; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D004247:DNA