title: Granulocyte colony-stimulating factor receptor mRNA upregulation is an immediate early marker of myeloid differentiation and exhibits dysfunctional regulation in leukemic cells.
abstract: The identification of early markers of myeloid differentiation can facilitate an understanding of how differentiation is arrested in leukemogenesis. Using murine bone marrow and the granulocyte-precusor cell line 32Dc13, we show that message for the granulocyte colony-stimulating factor receptor (G-CSFR) is upregulated by G-CSF in an immediate early fashion that is specific to the differentiation pathway and is antagonized by interleukin-3. We further show that G-CSFR message is superinduced by cycloheximide and that these patterns of regulation are altered in leukemic cell lines. In particular, the v-abl oncogene product supresses both ligand-mediated upregulation and superinduction of the G-CSFR gene.
authors.affiliation: Department of Medicine, University of Pittsburgh School of Medicine, PA.
authors.forename: R A
authors.initials: RA
authors.lastname: Steinman
authors.forename: D J
authors.initials: DJ
authors.lastname: Tweardy
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D015973:Gene Expression Regulation, Leukemic; D016313:Genes, abl; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D007938:Leukemia / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D007377:Interleukin-3; D012333:RNA, Messenger; D016188:Receptors, Granulocyte Colony-Stimulating Factor