Intestinal lactase: maturational excess expression of mRNA over enzyme protein.
The mechanism of decline of intestinal lactase during mammalian development remains uncertain. Despite a major loss of catalytic activity, lactase mRNA appears to persist at detectable concentrations in adult rats. We quantified lactase activity, total lactase protein, and lactase mRNA in rats aged 7, 11, 15, 18, 22, 30, and 60 days using the 7S ribosomal RNA as the developmental control. The active lactase fraction was 0.81 of total lactase for all age groups except 60-day-old animals, in which it declined to 0.60 (P = 0.004), indicating that conversion of active lactase to inactive species contributed to the lower activity in the adult. Northern blots revealed a single discrete 6.8-kb message at all ages. Although lactase activity and immunoprotein decreased coordinately to a minimum by day 30 (20% of the 7-day value), lactase mRNA doubled to a maximum at day 22 and was maintained at 7-day concentrations even in 60-day adults. The lactase mRNA-to-protein ratio was low at 7 days (0.19) but more than doubled (0.50) by 22 days, achieved a fivefold increase (1.0) by 30 days, and persisted at 0.77 in adults. The relative excess of lactase message during maturation suggests that translational or post-translational events may be paramount in the developmental regulation of lactase gene expression.
Nudell
D M
DM
Department of Medicine (Gastroenterology), Stanford University School of Medicine, California 94305.
Santiago
N A
NA
Zhu
J S
JS
Cohen
M L
ML
Majuk
Z
Z
Gray
G M
GM
D000375:Aging / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D007583:Jejunum / Q000201:enzymology* / Q000254:growth & development; D007668:Kidney / Q000201:enzymology; D043322:Lactase; D008099:Liver / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D001616:beta-Galactosidase / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism
D017931:DNA Primers; D012333:RNA, Messenger; D043322:Lactase; D001616:beta-Galactosidase