Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection.
Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.
Tamburrini
E
E
Department of Infectious Diseases, Università Cattolica, Rome, Italy.
Mencarini
P
P
De Luca
A
A
Antinori
A
A
Visconti
E
E
Ammassari
A
A
Ortona
L
L
Ortona
E
E
Siracusano
A
A
Vicari
G
G
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis*; D006801:Humans; D011010:Pneumocystis / Q000235:genetics*; D016720:Pneumocystis Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*
D004271:DNA, Fungal