Growth suppression of normal mammary epithelial cells by wild-type p53.
We examined the status of the p53 gene in the HC11 normal mammary epithelial cells. Two mutations were identified: a Cys to Trp change at codon 138 and a microdeletion of codon 123 to 130 resulting from mutation of the splice acceptor site. These two mutations were independent, and no wild-type p53 allele was found. Introduction of wt-p53 strongly inhibited growth in monolayer. Thus, the absence of wt-p53 can be sufficient for the immortalization of mammary cells.
Merlo
G R
GR
Friedrich Meischer Institute, Basel, Switzerland.
Venesio
T
T
Taverna
D
D
Callahan
R
R
Hynes
N E
NE
D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D019187:Cadmium Compounds; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D003062:Codon; D004847:Epithelial Cells; D005091:Exons; D016158:Genes, p53; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000166:cytology*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012377:Rodentia; D017384:Sequence Deletion; D013431:Sulfates; D014162:Transfection
D019187:Cadmium Compounds; D003062:Codon; D013431:Sulfates; D002104:Cadmium; C037123:cadmium sulfate