title: Rapid capillary gel electrophoresis of proteins.
abstract: The rapid separation of sodium dodecyl sulphate-protein complexes according to their molecular masses (M(r)) by capillary gel electrophoresis is described. Using commercial equipment, standard proteins with M(r) in the range 29,000-97,400 were resolved to the baseline in less than 2 min by utilizing a separation distance of 7 cm. A linear relationship between migration time and log M(r) was found and rapid determination of the molecular mass of light and heavy chains of human immunoglobulin G is reported. The results are compared with applications using longer separation distances, showing that rapid and efficient analysis and adequate resolution can be obtained by using short separation distances.
authors.affiliation: Westfälische Wilhelms-Universität Münster, Institut für Biochemie, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Lausch
authors.forename: T
authors.initials: T
authors.lastname: Scheper
authors.forename: O W
authors.initials: OW
authors.lastname: Reif
authors.forename: J
authors.initials: J
authors.lastname: Schlösser
authors.forename: J
authors.initials: J
authors.lastname: Fleischer
authors.forename: R
authors.initials: R
authors.lastname: Freitag
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D008970:Molecular Weight; D010047:Ovalbumin / Q000302:isolation & purification; D010763:Phosphorylase b / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
chemical_list: D007074:Immunoglobulin G; D011506:Proteins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D010763:Phosphorylase b; D002256:Carbonic Anhydrases