Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells.
Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS mRNA is associated with this translational regulation. In this study, an immunoprecipitation-reverse transcription-PCR technique was used to identify a TS ribonucleoprotein (RNP) complex in cultured human colon cancer cells. Using antibodies specific for TS protein, we show that TS is complexed in vivo with its own TS RNA. Furthermore, evidence demonstrating a direct interaction between the mRNA of the nuclear oncogene c-myc and TS protein is presented.
Chu
E
E
NCI-Navy Medical Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892.
Voeller
D M
DM
Jones
K L
KL
Takechi
T
T
Maley
G F
GF
Maley
F
F
Segal
S
S
Allegra
C J
CJ
D001483:Base Sequence; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*; D013940:Thymidylate Synthase / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D017931:DNA Primers; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012261:Ribonucleoproteins; D013940:Thymidylate Synthase