A carrageenan hydrogel stabilized colloidal gold multi-enzyme biosensor electrode utilizing immobilized horseradish peroxidase and cholesterol oxidase/cholesterol esterase to detect cholesterol in serum and whole blood.
The preparation of two immobilized enzyme electrodes is described. One electrode contains horseradish peroxidase absorbed to colloidal gold and deposited on a glassy carbon electrode along with cholesterol oxidase entrapped in a carrageenan hydrogel. The second electrode also includes cholesterol esterase entrapped in the carrageenan. The incorporation of ferrocene or ferrocenecarboxylic acid mediator is brought about by either evaporation on the glassy carbon electrode or, in the latter case, entrapment in the carrageenan hydrogel. Amperometric signal generation results from the HRP catalyzed turnover of H2O2, a secondary product of the cholesterol oxidase catalyzed oxidation of cholesterol. Use of these enzyme electrodes makes cholesterol detection possible in human serum, low density lipoprotein, and whole blood.
Crumbliss
A L
AL
Department of Chemistry, Duke University, Durham, NC 27706.
Stonehuerner
J G
JG
Henkens
R W
RW
Zhao
J
J
O'Daly
J P
JP
D015374:Biosensing Techniques; D002351:Carrageenan; D002784:Cholesterol / Q000097:blood*; D002789:Cholesterol Oxidase; D004566:Electrodes; D004800:Enzymes, Immobilized; D017979:Gold Colloid; D006735:Horseradish Peroxidase; D006801:Humans; D002787:Sterol Esterase
D004800:Enzymes, Immobilized; D017979:Gold Colloid; D002351:Carrageenan; D002784:Cholesterol; D002789:Cholesterol Oxidase; D006735:Horseradish Peroxidase; D002787:Sterol Esterase