Effect of acute ethanol dosage on nucleotide levels in the rat jejunum: relationship to protein synthesis.
The effects of an acute dose of ethanol (75 mmol/kg body weight; i.p.) on nucleotide levels in the rat jejunum were investigated. After 2.5 hr, ethanol exposure significantly reduced ATP and GTP contents and increased ADP and GDP contents. There were no statistically significant effects on pyrimidine nucleotides or IMP. Fractional rates of jejunal protein synthesis were also significantly reduced by ethanol. It was concluded that purine nucleotides are sensitive to acute ethanol administration in the rat jejunum. This may explain why rates of protein synthesis are decreased in the jejunum, as these nucleotides play a key role in polypeptide formation.
Marway
J S
JS
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London, U.K.
Rodrigues
L M
LM
Griffiths
J R
JR
Preedy
V R
VR
D000435:Alcoholic Intoxication / Q000503:physiopathology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D007583:Jejunum / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D009711:Nucleotides / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010649:Phenylalanine / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D009711:Nucleotides; D011506:Proteins; D010649:Phenylalanine