Establishment and characterisation of murine cells constitutively expressing the fusion, nucleoprotein and matrix proteins of measles virus.
To advance our understanding of the immunobiology of measles virus (MV) infections, we have investigated the possibility of establishing cell lines constitutively expressing the individual MV antigens. In contrast to previously published studies, we show that it is possible to establish cell lines expressing high levels of fusion (F), nucleoprotein (NP) and matrix (M) MV proteins. Once cloned, the cell lines were stable with high levels of expression for more than six months. The size and cell distribution of the NP and F proteins were similar to those observed in MV- or vaccinia-MV recombinant-infected cells. In contrast, the distribution of the M protein, although being similar to that of MV-infected cells, differed from that of Vaccinia-M recombinant virus-infected cells. Preliminary results suggest that these cell lines will be useful tools for studying the contribution of individual MV antigens to the cell-mediated immune response to this virus.
Beauverger
P
P
Unité d'Immunologie et Stratégie Vaccinale, Institut Pasteur de Lyon, France.
Buckland
R
R
Wild
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors; D006239:Haplotypes; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics; D007739:L Cells / Q000382:microbiology*; D008459:Measles virus / Q000235:genetics* / Q000276:immunology; D051379:Mice; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D015531:Radioimmunoprecipitation Assay; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics; D014760:Viral Fusion Proteins / Q000235:genetics* / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D018076:DNA, Complementary; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus; D006903:Hydroxymethylglutaryl CoA Reductases