title: Stimulation of phosphoinositide hydrolysis by glyceraldehyde-3-phosphate in electroporated HIT-T15 cells.
abstract: HIT-T15 cells labeled with myo-[3H] inositol were permeabilized by electroporation and subsequently stimulated with various glycolytic intermediates in the presence of 20 mM LiCl in a buffer mimicking cytosolic ionic composition. Of the various glycolytic intermediates tested, only D-glyceraldehyde-3-phosphate (G3-P) stimulated the formation of labeled inositol phosphates. The half-maximal response to G3-P occurred at a concentration of 0.75 mM. Formation of inositol phosphates in electroporated cells was also observed in response to GTP. G3-P further potentiated the formation of inositol phosphates in response to GTP, however, the interaction between G3-P and GTP was additive rather than synergistic, indicating that G3-P stimulates phosphoinositide hydrolysis in a manner different than the receptor mediated GTP-dependent activation of phospholipase C. The potentiation of the GTP response by G3-P did not appear to involve inhibition of the GTPase activity of a phosphoinositide-specific G protein, since G3-P also potentiated the formation of inositol phosphates in response to GTP-gamma-S or NaF in a nearly additive manner.
authors.affiliation: Department of Biological Sciences, St. John's University, Jamaica, New York 11439.
authors.forename: R
authors.initials: R
authors.lastname: Gonzalez
authors.forename: R S
authors.initials: RS
authors.lastname: Rana
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D006224:Cricetinae; D018274:Electroporation; D020558:GTP Phosphohydrolases / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000502:physiology; D005986:Glyceraldehyde 3-Phosphate / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D010716:Phosphatidylinositols / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D010716:Phosphatidylinositols; D005986:Glyceraldehyde 3-Phosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins