Restoration by bromocriptine of glucocorticoid receptors and glucocorticoid negative feedback on prolactin secretion in estrogen-induced pituitary tumors.
We previously reported a reduction of glucocorticoid receptors (GCR) in diethylstilbestrol-induced pituitary tumors (DES-T) in rats. Presently, we found that bromocriptine (BROM) treatment increased the levels of GCR in DES-T, demonstrated by steroid binding assays and immunocytochemistry using a monoclonal antibody against the type II GCR. We also found that the high content of nuclear estradiol receptors in the adenomata and the elevated levels of PRL in serum of DES-T were significantly reduced after BROM treatment. In parallel studies, PRL secretion was measured after administration of ether stress. In controls, serum PRL markedly increased after ether and this effect was blunted by prior dexamethasone (DEX) administration, due to the steroid negative feedback on PRL secretion. In animals with DES-T, ether stress had no effect on serum PRL, and the inhibition by DEX was lost unless they received BROM, which restored the negative feedback of DEX on serum PRL. Although increases of PRL titers in pituitary tumors may be due to estrogenic stimulation of lactotroph proliferation and function, coupled to absent dopaminergic inhibition on these cells, other mechanisms are possible. In this respect, inefficient steroid negative feedback on PRL synthesis due to down-regulation of GCR may contribute to hyperprolactinemia. This mechanism is supported from the restoration of GCR and steroid negative feedback on serum PRL by treatment of tumor-bearing rats with BROM.
Piroli
G
G
Laboratory of Neuroendocrine Biochemistry, Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.
Grillo
C
C
Ferrini
M
M
Diaz-Torga
G
G
Libertun
C
C
De Nicola
A F
AF
D000818:Animals; D000911:Antibodies, Monoclonal; D001971:Bromocriptine / Q000494:pharmacology*; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology; D004054:Diethylstilbestrol; D005246:Feedback; D005938:Glucocorticoids / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000139:chemically induced / Q000503:physiopathology*; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D001971:Bromocriptine; D004054:Diethylstilbestrol; D003907:Dexamethasone; D011388:Prolactin