[Establishment of a model of human gastric cancer with liver metastasis in nude mice].
An animal model of liver metastasis was established by injection of suspending tumor cells into subcapsule of spleen in nude mice. With additional mechanic disaggregation, single-tumor cell suspension was prepared by enzymatic disaggregation, including trypsin-collagenase disaggregate medium treatment, from the subcutaneous MGC 80-3 xeno-transplanted tumor and filtered through filter holders with different pore size. To investigate the effect of liver damage by carbon tetrachloride (CCL4) on the metastatic potential of MGC 80-3 cells injected intrasplenically, the nude mice were divided into 2 groups: Group 1 received intrasplenic injection of 1.25 x 10(6) MGC 80-3 cells without CCL4 treatment; Group 2 received intrasplenic injection of equal cells with CCL4 treatment. The incidence of liver metastasis was 60% in Group 1, but 100% in Group 2. The mean numbers of metastatic nodules per liver were 1.80 in Group 1, but 4.85 in Group 2. It is obvious that the incidence and nodules of liver metastasis were increased in the mice treated with CCL4. This is a useful model to study antimetastatic agents and metastatic behaviors.
Yang
S M
SM
Anticancer Research Centre, Xia Men University.
D002288:Adenocarcinoma, Mucinous / Q000556:secondary*; D000818:Animals; D002251:Carbon Tetrachloride; D004195:Disease Models, Animal; D005260:Female; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000473:pathology*
D002251:Carbon Tetrachloride