Bicuculline infused into the hamster ventral tegmentum inhibits, while sodium valproate facilitates, sexual receptivity.
Progesterone's (P) actions on both the ventral medial nucleus of the hypothalamus (VMH) and the ventral tegmental area (VTA) are essential for sexual receptivity in female hamsters. Evidence suggests that progesterone's actions in the hamster VMH may be genomic while those in the VTA may be mediated nongenomically, via GABAA. Ovariectomized female hamsters were bilaterally implanted with cannulae aimed toward the VTA. One week after surgery, animals were SC injected with 10 micrograms estradiol benzoate (EB) and 40 h later with 200 or 500 micrograms P. At hour 43.5, 50 ng bicuculline, a GABAA antagonist, was infused into each available cannula. Control animals received 0.5 microliter sterile saline vehicle, or no infusion. At hour 44, animals were tested for sexual receptivity in an observation arena with a sexually experienced male. Histology revealed that only animals with bicuculline infused into the VTA had reduced lordosis durations compared to controls. Other animals, primed with EB and 200 micrograms progesterone, showed a facilitation of sexual receptivity after infusion into the VTA of 50 micrograms sodium valproate, a GABAA transaminase inhibitor. These results suggest that GABAA plays a necessary role in the mechanism of progesterone's actions on sexual receptivity in hamster VTA.
Frye
C A
CA
Psychology Department, Tufts University, Medford, MA 02155.
Mermelstein
P G
PG
DeBold
J F
JF
D000818:Animals; D001640:Bicuculline / Q000008:administration & dosage / Q000494:pharmacology*; D006224:Cricetinae; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D058787:GABA-A Receptor Antagonists; D008647:Mesocricetus; D010052:Ovariectomy; D011187:Posture / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology; D018080:Receptors, GABA-B / Q000187:drug effects; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D013681:Tegmentum Mesencephali / Q000033:anatomy & histology; D014635:Valproic Acid / Q000494:pharmacology*
D058787:GABA-A Receptor Antagonists; D018080:Receptors, GABA-B; D011374:Progesterone; D004958:Estradiol; D014635:Valproic Acid; D001640:Bicuculline