title: Elements of the yeast pheromone response pathway required for filamentous growth of diploids.
abstract: Transmission of an external signal from receptors to downstream targets is often mediated by a conserved set of protein kinases that act in sequence (a kinase cascade). In haploid strains of Saccharomyces cerevisiae, a signal initiated by peptide pheromones is transmitted through this kinase cascade to a transcription factor STE12, which is required for the expression of many mating-specific genes. Here it was shown that in diploids some of the same kinases and STE12 are required for filamentous growth, but the pheromone receptors and guanosine triphosphate-binding protein are not required for filament formation. Thus, a similar kinase cascade is activated by different signals in haploids and diploids and mediates different developmental outcomes in the two cell types.
authors.affiliation: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: C A
authors.initials: CA
authors.lastname: Styles
authors.forename: G R
authors.initials: GR
authors.lastname: Fink
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D005800:Genes, Fungal; D000072235:Mating Factor; D009154:Mutation; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D010641:Phenotype; D011494:Protein Kinases / Q000378:metabolism*; D051964:Receptors, Mating Factor; D018000:Receptors, Peptide / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D010455:Peptides; D051964:Receptors, Mating Factor; D018000:Receptors, Peptide; C489241:STE12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000072235:Mating Factor; D011494:Protein Kinases; D019204:GTP-Binding Proteins