Five palmitoylated polypeptides in the 50 KDa range are not recognized by an antibody against ribulose-biphosphate-carboxylase-oxygenase in Chlamydomonas reinhardtii.
After incubation of Chlamydomonas reinhardtii cells with radioactive palmitic acid several labelled bands appeared after gel electrophoresis of delipidated protein extract. Among them, two bands (a major and a minor one) were detected in the 50 KDa range, which is the region where the LSU of the Rubisco (large sub-unit of the ribulose-biphosphate-carboxylase-oxygenase) was also found. Careful analyses by two-dimensional gel electrophoresis have shown that the five palmitate-labelled polypeptides detected in this region do not match with polypeptides immunoreacting with antibody against Rubisco. In addition, polypeptides labelled by palmitate cannot be immunoprecipitated with the same antibody further demonstrating that, in C. reinhardtii, the large sub-unit of Rubisco is not palmitoylated but unindentified proteins.
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Equipe de Biochimie des Membranes Végétales, URA 1128, CNRS, Gif-sur-Yvette, France.
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D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000201:enzymology*; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D008074:Lipoproteins / Q000276:immunology / Q000302:isolation & purification; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012273:Ribulose-Bisphosphate Carboxylase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
D008074:Lipoproteins; D010169:Palmitic Acids; D019308:Palmitic Acid; D012273:Ribulose-Bisphosphate Carboxylase