Characterization of a human chromosome 4 flow-sorted cosmid library.
A cosmid library has been constructed from a hamster x human hybrid cell line and gridded into 270 microtiter plates containing a total of 25,920 single colonies. Approximately 84% of the recombinants contain human material, with an average length of 29 kb. This library represents a nearly three-fold coverage of human chromosome 4. We investigated this library for presumptive genes, using a set of oligonucleotides detecting SpI and splice-site consensus sequences. The presence of simple repeat motifs was investigated in the cosmids using the oligonucleotides (GGATTT)3, (GGAT)4, (CAC)5, (GCC)5, (AGC)5, (GATA)4, (GACA)4, and (CA)8 as hybridization probes.
Riess
O
O
Department of Molecular Human Genetics, Ruhr-University, Bochum FRG.
Siedlaczck
I
I
Kredtke
S
S
Melmer
G
G
Epplen
J T
JT
Deaven
L L
LL
D000818:Animals; D001483:Base Sequence; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003360:Cosmids; D006224:Cricetinae; D005434:Flow Cytometry; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012091:Repetitive Sequences, Nucleic Acid