[Effect of radix salviae miltiorrhizae on the mitotic activity of osteoblast-like cells isolated from chicken embryo calvariae cultured in vitro].
Chicken embryo calvariae cultured in vitro, could yield 3 types of osteoblast-like cells, which differed in morphology. They were the spindle-shaped cells, globular cells, and squamous cells. Of these, the squamous cell was the chief cellular component, while both the spindle-shaped cells and the globular cells gradually transformed into squamous cells. Administration of Radix Salviae Miltiorrhizae rendered more rapid growth of the osteoblast-like cells in the early stage of culture. After 17-day of culture, when both the control group cells and the treated, group cells became quite confluent, 3H-Thymidine labelling and radioautographic study revealed a very statistically significant increase in the number of cells, bearing silver grains over the nucleus area in the treated group, as compared with the control (P < 0.001). Since osteoblasts play a pivotal role in fracture healing, administration of Radix Salviae Miltiorrhizae can exert favourable influence on bone repair through increasing the number of osteoblasts.
Hu
M Z
MZ
Shanghai Institute of Traumatology and Orthopaedics.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo / Q000166:cytology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology*; D010936:Plant Extracts; D027561:Salvia miltiorrhiza; D012886:Skull / Q000196:embryology*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts