Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field samples.
Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.
Kyes
S
S
Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, England, U.K.
Craig
A G
AG
Marsh
K
K
Newbold
C I
CI
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D016054:DNA, Protozoan; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; C047712:S-antigen, Plasmodium falciparum