Volume regulation in human fibroblasts: role of Ca2+ and 5-lipoxygenase products in the activation of the Cl- efflux.
Trypsinized human skin fibroblasts in suspension perform regulatory volume decrease (RVD) after cell swelling in hypotonic medium. During RVD, 36Cl- efflux is dramatically increased and the cell membrane is depolarized, indicating the activation of Cl- channels. This activation of Cl- channels depends on extracellular as well as on intracellular Ca2+. The swelling-induced Cl- efflux and the RVD response are inhibited by the 5-lipoxygenase inhibitor ETH 615-139. Finally, following hypotonic treatment, cellular pH decreases. The pH decrease does not involve the Cl-/HCO3- exchange because it is independent of the external Cl- concentration.
Mastrocola
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Laboratory of Biological Chemistry A, August Krogh Institute, Copenhagen, Denmark.
Lambert
I H
IH
Kramhøft
B
B
Rugolo
M
M
Hoffmann
E K
EK
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D017920:Antiporters / Q000502:physiology; D001094:Arachidonate 5-Lipoxygenase / Q000502:physiology*; D002118:Calcium / Q000502:physiology*; D002452:Cell Count; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002478:Cells, Cultured; D018118:Chloride Channels / Q000187:drug effects / Q000502:physiology*; D027963:Chloride-Bicarbonate Antiporters; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007038:Hypotonic Solutions; D017998:Leukotriene D4 / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D012867:Skin / Q000166:cytology*
D017920:Antiporters; D018118:Chloride Channels; D027963:Chloride-Bicarbonate Antiporters; D007038:Hypotonic Solutions; D017998:Leukotriene D4; D001094:Arachidonate 5-Lipoxygenase; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D002118:Calcium