[The subcellular distribution of the glutathione system enzymes in the brain tissue of the rat].
Using the percoll density gradient method for rapid isolation of organelles the distribution of glutathione system enzymes in the rat brain was characterized including glutathione S-transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR). In the fraction of mitochondria, the shares of GPx, GR, and GST were found to be as much as 92, 61, and 15% from their respective general activities. No enzymatic activity was found in lysosomes. Fractions of organelles with low density (microsomes, Golgi complex, cytoplasmic membranes) were cross-contaminated. Nevertheless, on the base of the established dynamics of distribution of the marker enzyme activity among various cell organelles it is suggested that, besides mitochondria, the glutathione system enzymes may be localized primarily in the endoplasmic reticulum, rather than in other organelles.
Kozhemiakin
L A
LA
Bulavin
D V
DV
Udintsev
A V
AV
Smirnov
V V
VV
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D003102:Colloids; D005979:Glutathione Peroxidase / Q000302:isolation & purification / Q000378:metabolism*; D005980:Glutathione Reductase / Q000302:isolation & purification / Q000378:metabolism*; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism*; D011205:Povidone; D051381:Rats; D012822:Silicon Dioxide; D013347:Subcellular Fractions / Q000201:enzymology
D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D011205:Povidone
Subkletochnyoe raspredelenie fermentov sistemy glutationa v tkani golovnogo mozga krysy.