[Comparative observation on several indices of nuclear damage induced by MMC in rat peripheral lymphocytes at G0 phase].
In this study the rat peripheral lymphocytes were treated with various doses of MMC, lymphocytes blood smear was prepared after 18h and was observed. The results showed that the nuclear damage of lymphocytes at Go phase was induced by MMC, and dose-dependent relationship was revealed for FMN (frequency of micronucleus), FIN (frequency of irregular nucleus) and FKN (frequency of karyorrhetic nucleus). Compared with the traditional micronucleus test, rat peripheral lymphocytes nuclear anomaly test seemed to be more sensitive and reasonable. It could therefore be considered as a short-term mutagentic test for preliminary screening and it was worthy of more study.
Kong
Z
Z
Department of Environmental Sciences, Nanjing University.
Wang
H
H
Liu
B
B
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D016685:Mitomycin / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D016192:Resting Phase, Cell Cycle
D016685:Mitomycin