Maintenance of viability and transport function after preservation of isolated rat hepatocytes in various simplified University of Wisconsin solutions.
Rat hepatocytes were preserved for 24 hr with high recovery and good maintenance of viability and transport function both in University of Wisconsin (UW) solution and in various simplified UW solutions. Cell quality is somewhat affected after 48 hr of preservation in both the original UW solution and the simplified solutions. ATP content and uptake rate of taurocholic acid are more sensitive markers of cell viability than Trypan blue exclusion or the MTT test. A much less expensive solution than UW, containing only K(+)-lactobionate, KH2PO4, MgSO4 and raffinose, can be used successfully for preservation of rat hepatocytes for 24 hr for drug transport studies.
Sandker
G W
GW
Department of Pharmacology and Therapeutics, University of Groningen, The Netherlands.
Weert
B
B
Merema
M T
MT
Kuipers
W
W
Slooff
M J
MJ
Meijer
D K
DK
Groothuis
G M
GM
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001692:Biological Transport; D002470:Cell Survival; D008099:Liver / Q000166:cytology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions; D013656:Taurocholic Acid / Q000378:metabolism; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014343:Trypan Blue
D012996:Solutions; D013656:Taurocholic Acid; D000255:Adenosine Triphosphate; D014343:Trypan Blue