Detection of Duchenne muscular dystrophy gene products in amniotic fluid and chorionic villus sampling cells.
We have examined the expression of several Duchenne muscular dystrophy (DMD) gene products in amniotic fluid (AF) and chorionic villus sampling (CVS) cells. Variable amounts of dystrophin could be detected in most CVS and AF samples by immunoprecipitation followed by Western blot analysis. PCR analysis demonstrated the presence of the muscle type dystrophin mRNA in all AF cell cultures. The brain type dystrophin mRNA was also detected in some of these cultures. These DMD gene transcripts are of fetal origin and are produced by most or all clonable AF cells. The results may facilitate the development of a method for prenatal diagnosis of DMD, based on the expression of the gene in AF and CVS cells.
Prigojin
H
H
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Brusel
M
M
Fuchs
O
O
Shomrat
R
R
Legum
C
C
Nudel
U
U
Yaffe
D
D
D000653:Amniotic Fluid / Q000166:cytology*; D015193:Chorionic Villi Sampling; D016189:Dystrophin / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D009136:Muscular Dystrophies / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012333:RNA, Messenger / Q000032:analysis
D016189:Dystrophin; D008565:Membrane Proteins; D012333:RNA, Messenger