Leukaemia arising in donor cells following allogeneic bone marrow transplantation for beta thalassaemia demonstrated by immunological, DNA and molecular cytogenetic analysis.
Combined DNA analysis, molecular cytogenetic and immunological techniques have been used to identify the donor origin of common acute lymphoblastic leukaemia (cALL) which arose in a male patient with beta thalassaemia major, 5 years after an allogeneic bone marrow transplant from his HLA-matched sister. The necessity of using multiple techniques in this and similar cases is emphasized and the possible mechanisms for the development of donor leukaemia and the leukaemic transformation of donor cells are discussed.
Katz
F
F
Department of Haematology and Oncology, Institute of Child Health, London.
Reeves
B R
BR
Alexander
S
S
Kearney
L
L
Chessells
J
J
D001853:Bone Marrow / Q000473:pathology; D016026:Bone Marrow Transplantation; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007223:Infant; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000209:etiology* / Q000235:genetics; D014184:Transplantation, Homologous; D017086:beta-Thalassemia / Q000601:surgery*
D004273:DNA, Neoplasm