Studies on antigenic relatedness of classic and variant strains of infectious bursal disease viruses.
Antigenic relatedness of six classic and variant strains of serotype 1 infectious bursal disease virus (IBDV) and one serotype 2 IBDV was investigated by Western blotting and enzyme-linked immunosorbent assay (ELISA) using polyclonal, monoclonal, and monospecific antibodies to single viral proteins (VP2 and VP3). All virus strains cross-reacted similarly, and the viruses were not distinguishable from each other by ELISA or Western blot analysis performed with polyclonal or non-neutralizing monoclonal and monospecific antibodies. Non-neutralizing antibodies against the VP2 (40 kilodaltons) reacted strongly with VP2 of classic and variant strains of serotype 1 and reacted weakly with VP2 of serotype 2 OH strain. This indicated that common antigens were recognized and that these epitopes were not strictly dependent on the native structure of the virus.
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Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Tsai
H J
HJ
Saif
Y M
YM
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000235:genetics; D001715:Bird Diseases / Q000276:immunology / Q000382:microbiology; D001717:Birds; D018175:Birnaviridae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D015153:Blotting, Western; D002213:Capsid / Q000276:immunology / Q000302:isolation & purification; D036022:Capsid Proteins; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007243:Infectious bursal disease virus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins