Characterization of an IgM Fc-binding receptor on human T cells.
The existence of an IgM receptor on human T cells has been suggested by T cell rosetting with IgM-coated erythrocytes. In this study we used immunofluorocytometry to demonstrate that 1) after short-term culture, a majority of the T cells can bind IgM at easily detectable levels, 2) human and mouse IgM preparations bind to human T cells but other Ig isotypes do not, 3) the IgM binding is saturable and inhibitable only by Ig of IgM isotype and 4) the Fc portion of IgM is involved in the binding to a protease-sensitive cell surface protein. Biochemical analysis of the T cell receptor for IgM reveals a cell surface protein of 60 kDa in comparison with the 58 kDa Fc mu receptors (Fc mu R) on B-lineage cells. Although Fc mu R expression is up-regulated after B cell activation, the reverse is true after T cell activation. In addition, the T cell Fc mu R is relatively resistant to phospholipase C treatment. These results indicate that T- and B-lineage cells express Fc mu R with different biologic characteristics.
Nakamura
T
T
Department of Medicine, University of Alabama at Birmingham.
Kubagawa
H
H
Ohno
T
T
Cooper
M D
MD
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism*; D007148:Immunoglobulin mu-Chains / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008970:Molecular Weight; D011961:Receptors, Fc / Q000737:chemistry / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007141:Immunoglobulin Fc Fragments; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D011961:Receptors, Fc; C035311:immunoglobulin M receptor