[Cross-allergenicity between rice and buckwheat antigens and immediate hypersensitive reactions induced by buckwheat ingestion].
Immediate hypersensitive reactions (IHR) induced by buckwheat ingestion are considered to be IgE-mediated. However we found 28 subjects without IHR to buckwheat ingestion out of 46 subjects who had positive RAST values for both buckwheat and rice antigens. The IHR-positive group showed significantly higher RAST values for buckwheat antigens (p < 0.01) but lower RAST values for rice antigens (p < 0.01) than did the IHR-negative group. RAST values for buckwheat and rice were significantly correlated with each other (p < 0.01) in the IHR-negative group, but not in the IHR-positive group. An effective dose-dependent inhibition was obtained in a RAST inhibition assay between homologous combinations of inhibitor and disc antigens such as rice and rice or buckwheat and buckwheat. The IHR-positive group showed no significant RAST inhibition between heterogeneous combinations of rice and buckwheat antigens. In contrast, the IHR-negative group showed a significant decrease in IgE binding even in the RAST inhibition assay between heterogenous combinations. These results led to the conclusion that there is cross-reactivity with IgE antibodies between buckwheat and rice and that IgE antibodies from IHR-negative subjects might recognize the epitopes on buckwheat antigens which cross react with rice antigens, whereas IgE antibodies from IHR-positive subjects might bind to buckwheat-specific epitopes.
Yamada
K
K
Department of Pediatrics, Fujita Health University School of Medicine.
Urisu
A
A
Kondou
Y
Y
Wada
E
E
Komada
H
H
Inagaki
Y
Y
Yamada
M
M
Torii
S
S
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D012275:Oryza / Q000276:immunology*; D011852:Radioallergosorbent Test; D014908:Triticum / Q000276:immunology*
D000485:Allergens; D007073:Immunoglobulin E