title: The effects of ionizing radiation on avian erythrocytes.
abstract: The effects of ionizing radiation were examined in terminally differentiated cells using nucleated chicken erythrocytes (RBCs) as the model. We used a hemolytic assay to score radiation damage to RBCs. Chicken RBCs received 0 to 100 Gy of radiation at dose rate of 10 Gy/min. Radiation-induced hemolysis occurred in a dose-dependent manner but not immediately after irradiation. Hemolysis became apparent at 24 h after treatment. A threshold for radiation dose response was observed. At doses below 30 Gy, hemolysis in irradiated samples was indistinguishable from that in nonirradiated controls. A total dose of 100 Gy was used for the split-dose experiments. The results showed that chicken RBCs were able to repair radiation damage and that the half-time for maximum recovery was approximately 30 min at 36 degrees C. Recovery from gamma radiation was also affected by the interfraction temperature.
authors.affiliation: Department of Physiology and Biophysics, University of Illinois, Urbana 61801.
authors.forename: S W
authors.initials: SW
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Ducoff
mesh_terms: D000818:Animals; D002588:Cesium Radioisotopes; D002645:Chickens; D004307:Dose-Response Relationship, Radiation; D004912:Erythrocytes / Q000502:physiology / Q000528:radiation effects*; D006461:Hemolysis / Q000528:radiation effects*; D066298:In Vitro Techniques; D007700:Kinetics; D011839:Radiation, Ionizing; D013696:Temperature; D013997:Time Factors
chemical_list: D002588:Cesium Radioisotopes