Mononuclear phagocytic system in acute pancreatitis.
1. Functional alterations of the mononuclear phagocytic system (MPS) may be an important factor in the pathogenesis of infection in acute pancreatitis (AP). In the present study, MPS activity was investigated in rats and hepatic blood flow (HBF) was also determined. 2. A total of 122 male Wistar rats were divided into three groups: 1, AP group (N = 51); 2, sham-operated (SO) (N = 49); 3, intact group (IG) (N = 22). AP was induced by retrograde injection of 0.5 ml of 2.5% sodium taurocholate saline into the main biliopancreatic duct under ketamine chloride anesthesia. SO animals were submitted to the same surgical steps as AP animals except for AP induction. 3. Each experimental group was subdivided into two subgroups. The first subgroup was submitted to the study of MPS activity as follows: each group was injected with colloidal 198Au and liver clearance parameters were determined 2 h (N = 11), 12 h (N = 10) and 24 h (N = 10) later in the AP group, and 2 h (N = 9), 12 h (N = 10) and 24 h (N = 11) later in the SO group. In the second subgroup, HBF was assessed using 131I-bromosulphalein at 2 h (N = 10) and 24 h (N = 10) in the AP group and at 2 h (N = 10) and 24 h (N = 10) in the SO group. The IG was submitted to both radioactive tracer studies. Each animal was used for only one experiment.(ABSTRACT TRUNCATED AT 250 WORDS)
Abdo
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EE
Departamento de Gastroenterologia, Faculdade de Medicina, Universidade de São Paulo, Brasil.
Gonçalez
Y
Y
Machado
M C
MC
Aguirre-Costa
P L
PL
Gonçalez
F
F
Sampietri
S N
SN
Pinotti
H W
HW
D000208:Acute Disease; D000818:Animals; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008102:Liver Circulation; D008297:Male; D010195:Pancreatitis / Q000209:etiology / Q000503:physiopathology*; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar