Isolation and characterization of a maize gene encoding chalcone flavonone isomerase.
We report here the first cloning of a chalcone flavonone isomerase gene (CHI) from maize. Northern blot experiments indicate that the maize CHI gene (ZmCHI1) is regulated in the pericarp by the P gene, a myb homologue. The ZmCHI1 gene encodes a 24.3 kDa product 55% and 58% identical to CHI-A and CHI-B from Petunia, respectively. This maize CHI1 gene has four exons and an intron-exon structure identical to the CHI-B gene of Petunia hybrida. RFLP mapping data indicate that some inbred lines contain two additional CHI-homologous sequences, suggesting an organization more complex than that found in Petunia or bean. The possibility that the additional CHI-homologous sequences are responsible for the lack of CHI mutants in maize will be discussed.
Grotewold
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Cold Spring Harbor Laboratory, NY 11724-2212.
Peterson
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T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002599:Chalcone / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics; D007887:Fabaceae / Q000235:genetics; D005419:Flavonoids / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D005809:Genes, Regulator; D019753:Intramolecular Lyases; D007535:Isomerases / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010858:Pigmentation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics; D010946:Plants, Medicinal; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D017931:DNA Primers; D004268:DNA-Binding Proteins; D005419:Flavonoids; D010940:Plant Proteins; D002599:Chalcone; D007535:Isomerases; D019753:Intramolecular Lyases; C019865:chalcone isomerase