title: Detection of heat shock protein in patients with insulin-dependent diabetes mellitus.
abstract: We have investigated whether antibodies to heat shock protein (hsp) 65 are present in sera from patients with insulin-dependent diabetes mellitus by using Mycobacterium leprae hsp65. Fifty-two sera from patients with IDDM, 36 from patients with unclassified insulin-treated diabetes mellitus and 41 from normal healthy controls were examined by ELISA assay. Seventeen (32.7%) out of 52 IDDM sera and 10 (27.8%) out of unclassified insulin-treated diabetic sera were positive for anti-Mycobacterium (anti-M. leprae) hsp65 antibodies while none of the healthy control sera were positive. Based on western blot analysis, 12 of the 17 IDDM sera and 1 of 2 sera from the unclassified insulin-treated diabetics were positive for anti-M.leprae hsp65 antibodies while all normal control sera were negative. These results support the idea that hsp65 may play a role in the pathogenesis of IDDM. Future studies are necessary to elucidate the role of hsp65 in the pathogenesis of IDDM.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Takei
authors.forename: T
authors.initials: T
authors.lastname: Kasatani
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: A
authors.initials: A
authors.lastname: Kasuga
authors.forename: A
authors.initials: A
authors.lastname: Shimada
authors.forename: T
authors.initials: T
authors.lastname: Maruyama
authors.forename: T
authors.initials: T
authors.lastname: Saruta
authors.forename: H
authors.initials: H
authors.lastname: Nomaguchi
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D015153:Blotting, Western; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D009166:Mycobacterium leprae / Q000276:immunology / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D006360:Heat-Shock Proteins