[Detection of secretory acid proteinase enzyme from Candida strains on protein supplemented agar media].
In this study, we identified the species of 130 candida strains isolated from various clinical specimens, and we also investigated the secretory acid proteinase activity of these strains on hemoglobin, albumin and casein containing agar media. In 65 of 75 C. albicans, 8 of 15 C. tropicalis, 5 of 15 C. pseudotropicalis, in 3 C. stellatoidea and in 2 C. glabrata strains enzyme activity was shown. We couldn't detect proteinase activity in any of the 7 C. krusei, 7 C. parapsilosis and in 6 C. guilliermondii strains. Enzyme activity was shown to be inhibited when proteolytic strains were treated with specific inhibitor pepstatin-A.
Cerikcioğlu
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N
Mikrobiyoloji Uzmani, Hacettepe Universitesi, Tip Fakültesi Mikrobiyoloji Anabilim Dali.
Alaçam
R
R
D000418:Albumins / Q000378:metabolism*; D016282:Aspartic Acid Endopeptidases / Q000032:analysis*; D002175:Candida / Q000187:drug effects / Q000201:enzymology*; D002364:Caseins / Q000378:metabolism*; D003470:Culture Media; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D010436:Pepstatins / Q000494:pharmacology
D000418:Albumins; D002364:Caseins; D003470:Culture Media; D006454:Hemoglobins; D010436:Pepstatins; C014195:Streptomyces pepsin inhibitor; D016282:Aspartic Acid Endopeptidases; C031375:pepstatin
Kandida suşlarinda salgisal asit proteinaz enzim varliğinin proteinli agar besiyerlerinde gösterilmesi.