title: Primordial germ cells in the primitive streak stages chick embryo as studied by scanning electron microscopy.
abstract: Chick embryo primordial germ cells (PGCs) at stages 4-8 were localised in the late hypoblast layer. PAS staining confirmed their distribution in a large arc at the anterior border of the area pellucida/area opaca. By scanning electron microscopy the PGCs were seen as spherical cells within the late hypoblast layer. Individual cells separated from this layer and collected in groups of 2-10 in shallow pockets in the hypoblast layer prior to their migration. The pockets containing PGCs were clearly visible by light and scanning electron microscopy and demarcated the germinal ridge at stages 4-8.
authors.affiliation: Department of Anatomy, University of Leicester, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: England
authors.forename: G
authors.initials: G
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D002469:Cell Separation; D002642:Chick Embryo / Q000648:ultrastructure*; D004707:Endoderm / Q000648:ultrastructure; D005775:Gastrula / Q000648:ultrastructure; D005854:Germ Cells / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D010503:Periodic Acid-Schiff Reaction