title: The determination of hydroxydopamines and other trace amines in the urine of parkinsonian patients and normal controls.
abstract: 5- and 6-Hydroxydopamine, which we had earlier identified as naturally occurring amines in human urine, were quantified in Parkinson's patients treated with L-DOPA, Parkinson's patients whose treatment did not include L-DOPA and in age matched controls. Analysis was carried out by GC-MS of the ditrifluoromethylbenzoyl-trimethylsilyl (DTFMB-TMS) derivatives of the compounds. The concentrations of 5- and 6-hydroxydopamine in the urine of DOPA treated Parkinson's patients were significantly higher than the concentrations from patients not treated and from normal controls. Urinary dopamine levels were greatly elevated in DOPA treated Parkinson's patients while p-tyramine levels were suppressed. No marked differences were seen between the three groups in terms of the urinary concentrations of any of the other amines measured.
authors.affiliation: Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow.
authors.forename: R
authors.initials: R
authors.lastname: Andrew
authors.forename: D G
authors.initials: DG
authors.lastname: Watson
authors.forename: S A
authors.initials: SA
authors.lastname: Best
authors.forename: J M
authors.initials: JM
authors.lastname: Midgley
authors.forename: H
authors.initials: H
authors.lastname: Wenlong
authors.forename: R K
authors.initials: RK
authors.lastname: Petty
mesh_terms: D015306:Biogenic Monoamines / Q000652:urine*; D004298:Dopamine / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006892:Hydroxydopamines / Q000652:urine*; D007980:Levodopa / Q000627:therapeutic use; D009638:Norepinephrine / Q000652:urine; D016627:Oxidopamine / Q000652:urine*; D010300:Parkinson Disease / Q000188:drug therapy / Q000652:urine*; D014439:Tyramine / Q000652:urine
chemical_list: D015306:Biogenic Monoamines; D006892:Hydroxydopamines; C027802:5-hydroxydopamine; D007980:Levodopa; D016627:Oxidopamine; D004298:Dopamine; D009638:Norepinephrine; D014439:Tyramine