Detection of Mycoplasma gallisepticum infection in field samples using a species-specific DNA probe.
Species-specific DNA probes for Mycoplasma gallisepticum (MG) were compared with serologic and isolation procedures as a routine diagnostic tool on field specimens acquired from chicken flocks experiencing egg-production losses and suspected of MG infection. The MG DNA probe clearly identified MG directly from tracheal specimens within 2 days, unlike the 7 to 10 days required for culture procedures. Cross-reaction of MG with M. synoviae continues to be a stumbling block in the serum plate agglutination test.
Khan
M I
MI
Department of Pathobiology, College of Agriculture and Natural Resources, University of Connecticut, Storrs 06269-3089.
Kleven
S H
SH
D000372:Agglutination Tests; D000818:Animals; D002645:Chickens; D015342:DNA Probes; D005069:Evaluation Studies as Topic; D005260:Female; D006385:Hemagglutination Inhibition Tests; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000382:microbiology; D014132:Trachea / Q000382:microbiology
D015342:DNA Probes