Strings of contiguous modified pentanucleotides with increased DNA-binding affinity can be used for DNA sequencing by primer walking.
Modified oligonucleotides containing 5-methylcytidine and/or 2-aminoadenosine form tighter hybrids with DNA and are, therefore, more efficient primers for DNA sequencing as compared to their natural counterparts. Strings of contiguous modified pentanucleotides can be used for DNA sequencing by primer walking.
Azhikina
T
T
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Veselovskaya
S
S
Myasnikov
V
V
Potapov
V
V
Ermolayeva
O
O
Sverdlov
E
E
D000241:Adenosine / Q000031:analogs & derivatives; D000577:Amides; D001483:Base Sequence; D003562:Cytidine / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers / Q000138:chemical synthesis; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010756:Phosphoric Acids; D013329:Structure-Activity Relationship
D000577:Amides; D017931:DNA Primers; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010756:Phosphoric Acids; C033854:2-aminoadenosine; D003562:Cytidine; D004247:DNA; C011067:phosphoramidic acid; D000241:Adenosine; C016568:5-methylcytidine