Role of isoprenoid metabolism in chemotactic peptide receptor-mediated G protein activation.
The role of isoprenylation in formyl peptide receptor-mediated G protein activation was studied using plasma membranes isolated from normal HL-60 granulocytes and from cells in which isoprenylation was inhibited with mevastatin. Plasma membrane expression of formyl peptide receptors and G protein beta subunits, but not alpha i2 and alpha i3, was significantly reduced by inhibition of isoprenylation. This reduced expression resulted in impaired basal and fMet-Leu-Phe-stimulated G protein activation.
McLeish
K R
KR
Veterans Administration Medical Center, Louisville, KY.
Lederer
E D
ED
Klein
J B
JB
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000924:Anticholesteremic Agents / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002772:Cholera Toxin / Q000378:metabolism / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008148:Lovastatin / Q000031:analogs & derivatives / Q000494:pharmacology; D046911:Macromolecular Substances; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009243:NAD / Q000378:metabolism; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000187:drug effects / Q000378:metabolism*; D018000:Receptors, Peptide / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000378:metabolism / Q000494:pharmacology
D000924:Anticholesteremic Agents; D046911:Macromolecular Substances; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D010566:Virulence Factors, Bordetella; D009243:NAD; C012258:mevastatin; D000246:Adenosine Diphosphate Ribose; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D009240:N-Formylmethionine Leucyl-Phenylalanine; D006160:Guanosine Triphosphate; D002772:Cholera Toxin; D008148:Lovastatin; D019204:GTP-Binding Proteins