[Mapping of cloned fragments of the macrophage-stimulating factor receptor gene (FMS) from a non-amplified library of human leukocyte genes].
For investigation of FMS gene polymorphism and mutations that reveal functionally meaning in leukemia and myelodysplastic disorders the overlapping recombinants lambda-clones inserted by FMS gene fragments have been obtained from human leukocyte genomic library in the EMBL 3A phage by using oligonucleotide prode (27 nucleotides) based on 12 exon of the FMS gene. 15 DNA probes were prepared by subcloning the lambda-clones obtained in the pBSKS+ plasmid. The probes obtained allow to analyse extracellular, transmembrane and tyrosine kinase regions of the FMS gene independently.
Aleksandrova
N M
NM
Kutina
O A
OA
Pantin
V I
VI
Grineva
N I
NI
D003001:Cloning, Molecular; D005091:Exons; D005784:Gene Amplification; D015723:Gene Library; D015321:Gene Rearrangement; D005822:Genetic Vectors; D006801:Humans; D007938:Leukemia / Q000235:genetics; D007962:Leukocytes / Q000502:physiology*; D009154:Mutation; D015345:Oligonucleotide Probes; D011110:Polymorphism, Genetic; D011289:Preleukemia / Q000235:genetics; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics*; D015183:Restriction Mapping
D015345:Oligonucleotide Probes; D016186:Receptor, Macrophage Colony-Stimulating Factor
Kartirovanie klonirovannykh fragmentov gena retseptora koloniestimuliruiushchego faktora makrofagov (FMS) iz neamplifitsirovannoĭ biblioteki genov leĭkotsitov cheloveka.