Antigen-independent adhesion of CD4 CD45RA T cells from cord blood.
Antigen-independent adhesion of resting adult CD4+ CD45RO+ T cells to B lymphocytes has been shown to be transient and can be down-regulated by CD4 major histocompatibility complex (MHC) class II molecule interactions. Conversely, adhesion of adult CD4+ CD45RA+ subpopulation to B cells is not regulated by ligands of CD4. We have investigated the regulation of adhesion of cord blood CD45RA+ CD4+ T lymphocytes. In contrast to adult CD45RA+ CD4+ T cells, cord blood CD45RA+ CD4+ T cells were strongly sensitive to the down-regulation of adhesion mediated by the CD4-HLA class II interaction, since adhesion to MHC class II(+) B cells was transient and inhibited by an anti-CD4 antibody. In addition, human immunodeficiency virus gp160, synthetic gp106-derived peptides encompassing a CD4 binding site inhibited conjugate formation between cord blood CD45RA+ CD4+ T cells and B cells. Following activation of the cord blood CD4 T cells by an anti-CD3 antibody, a conversion from a transient to a stable adhesion pattern of cord blood CD4 T cells to B cells occurred in 2 days. The reversal to a transient adhesion occurred at day 8 following anti-CD3 activation in correlation with a complete shift to a CD45RO phenotype of the cord blood CD4 T cells. These data suggest that CD4 T cell adhesion can be developmentally regulated.
Buzyn-Veil
A
A
INSERM U 132, Hôpital Necker-Enfants Malades, Paris, France.
Hivroz
C
C
Lecomte
O
O
Barbat
C
C
Mazerolles
F
F
Fischer
A
A
D000328:Adult; D000595:Amino Acid Sequence; D000941:Antigens / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology; D015704:CD4 Antigens / Q000032:analysis* / Q000502:physiology; D002448:Cell Adhesion; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D015686:Gene Products, env / Q000494:pharmacology; D019302:HIV Envelope Protein gp160; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis*; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*
D000941:Antigens; D015704:CD4 Antigens; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D000949:Histocompatibility Antigens Class II; D011498:Protein Precursors; D017493:Leukocyte Common Antigens