The pharmacologic evaluation of locomotor activity versus inflammatory parameters in rat adjuvant arthritis.
Adjuvant-induced arthritis (AA) is an experimental model of inflammatory joint disease in the rat which mimics rheumatoid arthritis. Although paw inflammation (e.g., swelling) is commonly used to monitor the efficacy of antiarthritic drugs, a reduction in locomotor function may provide a more sensitive evaluation of "functional disability" in AA rats. The purpose of the present study was to investigate the effect of dietary therapy with prednisolone or ibuprofen on locomotor activity as well as arthritic symptoms in established AA (days 20-42). AA rats demonstrated an increase in arthritis scores, spleen weights, fibrinogen, and WBC along with a reduction in locomotor function. Prednisolone (2 mg/kg/day) exhibited a positive therapeutic effect on all these parameters. Ibuprofen (50 mg/kg/day) consistently lowered arthritis scores and fibrinogen; however, locomotor function only improved on day 35. In conclusion, the measurement of locomotor activity in concert with other experimental parameters may provide a more meaningful evaluation of disease severity or improvement in AA.
Selph
J L
JL
Division of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, NC 27709.
Boncek
V M
VM
Soroko
F E
FE
Harris
T M
TM
Cochran
F R
FR
D000818:Animals; D001169:Arthritis, Experimental / Q000178:diet therapy / Q000188:drug therapy* / Q000503:physiopathology; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D007052:Ibuprofen / Q000627:therapeutic use*; D007596:Joints / Q000503:physiopathology*; D007958:Leukocyte Count / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects*; D009929:Organ Size / Q000187:drug effects; D011239:Prednisolone / Q000627:therapeutic use*; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000187:drug effects / Q000473:pathology
D005340:Fibrinogen; D011239:Prednisolone; D007052:Ibuprofen