title: Lectin histochemical study of bovine lingual glands.
abstract: Bovine lingual glands consist of mucous acini capped by demilunes. Information on the chemical structure of their secretory glycoconjugates was obtained by means of a battery of peroxidase-conjugated lectins with affinity for specific terminal sugars. Sialidase procedures followed by lectin staining were also used to visualize the sugar sequences. Stored secretions in mucous acinar cells contained fucose, N-acetylglucosamine, alpha and beta-N-acetylgalactosamine as terminal sugar residues and beta-galactose as penultimate sugar in a heterogeneous distribution. Demilunar cells failed to react with any of the lectins examined except that of Dolichos biflorus.
authors.affiliation: Istituto di Anatomia Normale degli Animali Domestici, Università degli Studi di Perugia, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Gargiulo
authors.forename: V
authors.initials: V
authors.lastname: Pedini
authors.forename: P
authors.initials: P
authors.lastname: Ceccarelli
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D010367:Arachis; D002417:Cattle; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D037102:Lectins; D008297:Male; D009092:Mucous Membrane / Q000166:cytology / Q000378:metabolism; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000032:analysis / Q000648:ultrastructure; D012470:Salivary Glands, Minor / Q000166:cytology* / Q000378:metabolism*; D030262:Soybean Proteins; D013025:Glycine max; D014059:Tongue / Q000166:cytology* / Q000378:metabolism*; D014909:Wheat Germ Agglutinins; D010835:Phytohemagglutinins
chemical_list: D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D005643:Fucose; D005690:Galactose; D006735:Horseradish Peroxidase; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011975:Receptors, Mitogen; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C036850:Ulex europaeus lectins; C036554:dolichos biflorus agglutinin; C028824:soybean lectin; D010835:Phytohemagglutinins