title: Analysis of N-acyl aminonaphthalene sulphonic acid derivatives with potential anti-human immunodeficiency virus activity by thin-layer chromatography and flame ionization detection.
abstract: A method for checking the purity of N-acyl aminonaphthalene disulphonic acid derivatives was required for a systematic study of the anti-human immunodeficiency virus activity of these agents. We describe the use of thin-layer chromatography and flame ionization detection for the separation of these compounds, which are difficult to analyse by conventional methods. All the samples were prepared in methanol solutions (1 microliter) containing 5 micrograms of aminonaphthalene derivative. These samples were applied to each type SIII Chromarod by a single injection and developed with pure methanol or a methanol-chloroform-ammonium hydroxide (35:55:10, v/v/v) solvent system.
authors.affiliation: Laboratoire des IMRCP, UA CNRS 470, Université Paul Sabatier, Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Madelaine-Dupuich
authors.forename: J
authors.initials: J
authors.lastname: Azema
authors.forename: B
authors.initials: B
authors.lastname: Escoula
authors.forename: I
authors.initials: I
authors.lastname: Rico
authors.forename: A
authors.initials: A
authors.lastname: Lattes
mesh_terms: D000998:Antiviral Agents / Q000032:analysis* / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D005410:Flame Ionization; D006678:HIV / Q000187:drug effects*; D009281:Naphthalenes / Q000032:analysis* / Q000494:pharmacology; D013451:Sulfonic Acids / Q000032:analysis* / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D009281:Naphthalenes; D013451:Sulfonic Acids