title: Chloride-depletion of photosynthetic water oxidase. No proton release during the second oxidation step, S2*==>S3*, and a transmembrane radical pair recombination from the third on.
abstract: Chloride depletion blocks the normal four-step progress of photosynthetic water oxidation. We studied proton release in chloride-depleted thylakoids which were dark-adapted and excited by flashing light. Proton release was blocked from the second flash on, possibly leaving an uncompensated positive charge in the catalytic centre. The reduction of P+680 by Tyrz was still very rapid (<< 10 microseconds). From the third flash on, P+680 was reduced more slowly (70 microseconds/200 microseconds), and by an electrogenic back-reaction. The uncompensated positive charge may be the reason why the rapid reduction of P+680 by Tyrz is prevented and the transmembrane charge-pair recombination is facilitated.
authors.affiliation: Biophysik, Universitt Osnabrück, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Lübbers
authors.forename: W
authors.initials: W
authors.lastname: Drevenstedt
authors.forename: W
authors.initials: W
authors.lastname: Junge
mesh_terms: D002712:Chlorides / Q000737:chemistry*; D003623:Dark Adaptation; D004563:Electrochemistry; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000737:chemistry / Q000502:physiology; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry*; D010788:Photosynthesis; D014867:Water / Q000737:chemistry*
chemical_list: D002712:Chlorides; D005609:Free Radicals; D014867:Water; D010088:Oxidoreductases