Involvement of cytochrome P-450 in conferring chloroquine resistance to the malarial parasite, Plasmodium falciparum.
The higher levels of cytochrome P-450 dependent enzyme activities reported earlier are traced to higher levels of cytochrome P-450 (CYPIIB1/B2 like) messenger RNA in the chloroquine resistant than the sensitive strains. The messenger RNA is also induced by phenobarbitone in the sensitive strain. Pretreatment with phenobarbitone affords partial protection to chloroquine toxicity in the sensitive strain and this is not due to a differential accumulation of the drug.
Surolia
N
N
Department of Biochemistry, Indian Institute of Science, Bangalore.
Karthikeyan
G
G
Padmanaban
G
G
D000818:Animals; D002738:Chloroquine / Q000378:metabolism / Q000633:toxicity*; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004351:Drug Resistance / Q000235:genetics*; D005091:Exons; D008715:Methionine / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D016053:RNA, Protozoan / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats
D012333:RNA, Messenger; D016053:RNA, Protozoan; D002738:Chloroquine; D003577:Cytochrome P-450 Enzyme System; D008715:Methionine; D010634:Phenobarbital