Characterization of the transforming domain of a feline sarcoma virus encoding a fgr-related tyrosine kinase.
We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes a fgr-related tyrosine kinase expressed as a gag-fgr fusion protein. The gag portion was removed and replication-competent expression vectors (RCAS) with inserted v-fgr sequences were established. Chicken embryo fibroblasts (CEF) were transfected and monitored for replication, integration and transcription of the proviral constructs. We demonstrated that transfected cells display morphological changes and are able to form colonies in soft-agar. This suggests that the gag portion of the fusion protein from TP1-FeSV is not necessary for the transformation of fibroblasts.
Weyrauch
G
G
Department of Experimental Tumorbiology, University of Muenster, Federal Republic of Germany.
Barnekow
A
A
D000818:Animals; D001665:Binding Sites; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts; D005814:Genes, Viral; D015750:Genes, gag; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D012367:RNA, Viral / Q000032:analysis; D015682:Retroviridae Proteins, Oncogenic / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D005253:Sarcoma Viruses, Feline / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D014162:Transfection
D004279:DNA, Viral; D012367:RNA, Viral; D015682:Retroviridae Proteins, Oncogenic; C052601:v-fgr protein, Feline sarcoma virus; D011505:Protein-Tyrosine Kinases