Molecular cloning of a novel, putative G protein-coupled receptor from sea anemones structurally related to members of the FSH, TSH, LH/CG receptor family from mammals.
Using oligonucleotide probes derived from consensus sequences of known vertebrate and invertebrate G protein-coupled receptors, we have cloned the cDNA for a presumed G protein-coupled receptor from sea anemones. This receptor shows a striking structural homology with members of the glycoprotein hormone (FSH, TSH, LH/CG) receptor family from mammals, including a very large, extracellular N terminus (18-25% sequence identity) and a 7 transmembrane region (44-48% sequence identity). As with the mammalian glycoprotein hormone receptor genes, the sea anemone receptor gene yields transcripts which can be alternatively spliced, thereby yielding a shortened receptor variant only containing the large extracellular (soluble) N terminus. All this is strong evidence that the putative glycoprotein hormone receptor from sea anemones is evolutionarily related to those from mammals. This is the first report showing that a putative glycoprotein hormone receptor occurs in invertebrates.
Nothacker
H P
HP
Centre for Molecular Neurobiology (ZMNH), University of Hamburg, Germany.
Grimmelikhuijzen
C J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005091:Exons; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry; D015723:Gene Library; D008322:Mammals; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry*; D011962:Receptors, FSH / Q000737:chemistry*; D011974:Receptors, LH / Q000737:chemistry*; D011989:Receptors, Thyrotropin / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D012615:Sea Anemones / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D015345:Oligonucleotide Probes; D011956:Receptors, Cell Surface; D011962:Receptors, FSH; D011974:Receptors, LH; D011989:Receptors, Thyrotropin; D019204:GTP-Binding Proteins