Actions of interleukin-4 on prostaglandin biosynthesis at the chorion-decidual interface.
OBJECTIVE
We determined the effects of interleukin-4 on chorion and decidual prostaglandin production.


METHODS
Chorion and decidual cells from term placentas were grown to confluence. Cells were then incubated with interleukin-4 either alone or with other known stimulants of prostaglandin production: interleukin-1 beta, epidermal growth factor, ionomycin, or phorbol 12-myristate 13-acetate. Prostaglandin E2 production was determined with a specific radioimmunoassay.


RESULTS
Interleukin-4 alone stimulated prostaglandin E2 production in chorion and decidual cells. Interleukin-4 significantly enhanced the stimulatory actions of phorbol 12-myristate 13-acetate, ionomycin, and epidermal growth factor but not interleukin-1 beta on prostaglandin E2 production.


CONCLUSIONS
Interleukin-4 stimulates prostaglandin E2 production by chorion and decidual cells. These data suggest that interleukin-4 production by immune effector cells in gestational tissues may contribute to the pathophysiologic features of preterm labor.
Adamson
S
S
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
Edwin
S S
SS
LaMarche
S
S
Mitchell
M D
MD
D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D003656:Decidua / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000502:physiology*; D015759:Ionomycin / Q000494:pharmacology; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015847:Interleukin-4; D015759:Ionomycin; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate