title: [Basic and clinical evaluation of EIA (pin immuno assay, PIA) kit for antithyroglobulin antibody (TGAb) and antimicrosomal (antiperoxidase) antibody (TMAb)].
abstract: Newly developed enzyme immunoassay kit (Pin Immuno Assay, PIA) for quantitative determination of serum antithyroglobulin antibody (TGAb) and antimicrosomal (-peroxidase) antibody (TMAb) was evaluated. The method utilizes the Sandwich ELISA principle with a unique micropin as solid phase coated with antigen. Reproducibilities assessed by intra- and interassay variation were less than 6.9% (CV) and 7.2% for TGAb or 4.2% and 6.0% for TMAb respectively. Changes in the first or second incubation time did not affect both TGAb and TMAb values. Upper normal limits obtained from 47 healthy subjects were 150 IU/ml for TGAb and 25 IU/ml for TMAb. Positive results in TGAb were obtained 60.0% in patients with Graves' disease and 80.0% in chronic thyroiditis and in TMAb 77.8% in Graves' disease and 66.7% in chronic thyroiditis. Patients with other autoimmune diseases such as SLE, RA were also found high incidence of positive results, 48.3% for TGAb and 65.0% for TMAb respectively. These results indicate that the nonradioisotopic assay technique for thyroid autoantibodies are useful in diagnosis of autoimmune diseases especially thyroid diseases.
authors.affiliation: Central Clinical Laboratory, Tokyo University School of Medicine.
authors.forename: H
authors.initials: H
authors.lastname: Uchimura
authors.forename: Y
authors.initials: Y
authors.lastname: Aono
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Takada
authors.forename: H
authors.initials: H
authors.lastname: Yamaguti
authors.forename: C
authors.initials: C
authors.lastname: Yanai
authors.forename: S
authors.initials: S
authors.lastname: Yoshinoya
authors.forename: A
authors.initials: A
authors.lastname: Okubo
authors.forename: T
authors.initials: T
authors.lastname: Mitsuhashi
authors.forename: K
authors.initials: K
authors.lastname: Kubota
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000175:diagnosis*; D002908:Chronic Disease; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007453:Iodide Peroxidase / Q000276:immunology*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D013959:Thyroid Diseases / Q000175:diagnosis*
chemical_list: D001323:Autoantibodies; D011933:Reagent Kits, Diagnostic; C006606:anti-thyroglobulin; D007453:Iodide Peroxidase