title: Internal ribosome entry site of encephalomyocarditis virus RNA is unable to direct translation in Saccharomyces cerevisiae.
abstract: To evaluate the potential of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) to promote efficient expression of foreign genes in the yeast, S. cerevisiae, we have constructed E. coli-yeast shuttle vectors in which the EMCV 5' non-coding region was fused to the reporter gene, human prothymosin alpha. Efficiency of translation of corresponding RNA transcripts in mammalian cell-free systems was highly dependent on the sequence context and/or position of the initiation codon. No translation of these IRES-dependent mRNAs occurred in S. cerevisiae.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
authors.forename: A G
authors.initials: AG
authors.lastname: Evstafieva
authors.forename: A V
authors.initials: AV
authors.lastname: Beletsky
authors.forename: A V
authors.initials: AV
authors.lastname: Borovjagin
authors.forename: A A
authors.initials: AA
authors.lastname: Bogdanov
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004686:Encephalomyocarditis virus / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012270:Ribosomes / Q000382:microbiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013947:Thymosin / Q000031:analogs & derivatives / Q000235:genetics; D016662:Virus Integration / Q000235:genetics*
chemical_list: D017931:DNA Primers; D011498:Protein Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; C040782:prothymosin alpha; D013947:Thymosin