Influence of age on IgE responsiveness to Dermatophagoides farinae: an immunoblot study.
IgE responsiveness to Dermatophagoides farinae body extract was compared by immunoblotting among four groups of mite-sensitive asthmatic children: group I aged 0-3, group II aged 4-7, group III aged 8-11 and group IV aged 12-15. In the group I subjects, the 15-kD component was bound by 88% of sera and strongly by 56% of sera. The 25-kD component was bound by 40% of sera, but most of the binding reactions were faint. IgE binding to other components was generally low. In the group II subjects, 15- and 25-kD components were bound by 96% and 56% of sera, 30-kD and 110-kD components by 76% and 60% of sera, respectively. In addition, 18-, 40-, 59- and 80-kD components were bound by more than 30% of sera. In group III and IV subjects, the frequencies of IgE binding to these components were not significantly changed as compared with those of group II subjects. The rabbit antisera against Der f I and Der f II specifically reacted with 25- and 15-kD components, respectively. These results suggest that the 15-kD Der f II allergen is the most important antigenic constituent associated with the early IgE response to house dust-mites in mite-reactive asthmatic children.
Shibasaki
M
M
Department of Pediatrics, University of Tsukuba, Japan.
Isoyama
S
S
Takita
H
H
D000293:Adolescent; D000375:Aging / Q000502:physiology*; D000485:Allergens / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology / Q000502:physiology*; D007223:Infant; D007231:Infant, Newborn; D008925:Mites / Q000276:immunology*
D000485:Allergens; D000941:Antigens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007073:Immunoglobulin E