How profilin promotes actin filament assembly in the presence of thymosin beta 4.
The role of profilin in the regulation of actin assembly has been reexamined. The affinity of profilin for ATP-actin appears 10-fold higher than previously thought. In the presence of ATP, the participation of the profilin-actin complex to filament elongation at the barbed end is linked to a decrease in the steady-state concentration of globular actin. This surprising effect is made possible by the involvement of the irreversible ATP hydrolysis accompanying actin polymerization. As a consequence, in the presence of thymosin beta 4 (T beta 4), low amounts of profilin promote extensive actin assembly off of the pool of actin-T beta 4 complex. When barbed ends are capped, profilin simply sequesters globular actin. A model is proposed for the function of profilin in actin-based motility.
Pantaloni
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D
Laboratoire d'Enzymologie Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Carlier
M F
MF
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003285:Contractile Proteins; D018260:Gelsolin / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008840:Microfilament Proteins / Q000378:metabolism / Q000502:physiology*; D051304:Profilins; D012756:Sheep; D013947:Thymosin / Q000494:pharmacology; D000099201:Thymosin beta(4)
D000199:Actins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003285:Contractile Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; D051304:Profilins; D013947:Thymosin; D000099201:Thymosin beta(4); C495738:PFN1 protein, human