Molecular cloning of a putative homolog of proline/arginine-rich antibacterial peptides from porcine bone marrow.
Screening of a porcine bone marrow cDNA library with a PCR-derived probe from rabbit LPS-binding protein CAP18 led to the discovery of two closely related clones. The longer, full-length cDNA clone encodes a 228 amino acid residue protein similar to the family of antibacterial/LPS-binding cationic peptides. In contrast to other hitherto discovered precursors of Pro/Arg-rich peptides from this family, they have a novel, unique structure of the C-terminal region of 100 amino acid residues with a repeating sequence of ten residues (FPPPNXPGPR, where X = V or F). These precursors could represent a part of the antibacterial peptide repertoire of porcine bone marrow.
Pungercar
J
J
Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Ljubljana, Slovenia.
Strukelj
B
B
Kopitar
G
G
Renko
M
M
Lenarcic
B
B
Gubensek
F
F
Turk
V
V
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001120:Arginine / Q000032:analysis*; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D011392:Proline / Q000032:analysis*; D055232:Proline-Rich Protein Domains; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013552:Swine; D054804:Cathelicidins
D000890:Anti-Infective Agents; D001120:Arginine; D018076:DNA, Complementary; D010455:Peptides; D011392:Proline; D011498:Protein Precursors; C084994:NPG4 protein, Sus scrofa; D054804:Cathelicidins