Tactics for the isolation of interleukin-2-induced immediate-early genes.
Interleukin-2 (IL-2), a 15 kDa protein secreted by antigen-activated T lymphocytes, plays a central role in the generation of an immune response. Initially characterized as a T cell growth factor, IL-2 has subsequently been found to act on all lymphoid cell types, inducing both their proliferation and functional differentiation. The effects of IL-2 are mediated by the activation of a specific cell-surface receptor (IL-2R), which is comprised of at least three distinct proteins. Early biochemical signaling events triggered upon IL-2R activation have recently begun to be elucidated, and a number of genes have been identified which are specifically induced by IL-2. The task now at hand is to link the biochemical signaling pathways activated by IL-2 with the regulation of gene expression, and to delineate the roles of these gene products in IL-2-mediated proliferation and differentiation.
Beadling
C
C
Department of Medicine, Dartmouth Medical School, Hanover, NH 03755.
Smith
K A
KA
D000818:Animals; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*
D007376:Interleukin-2