title: Percoll semen preparation enhances human oocyte fertilization in male-factor infertility as shown by a randomized cross-over study.
abstract: The aim of this study was to compare two methods of semen preparation: multiple tube swim-up and Percoll separation, using a randomized cross-over clinical study, in which sperm parameters, oocyte fertilization rates, embryo quality and cell stage were analysed. Overall, there was no difference between the two preparation methods in the normozoospermic cycles. In the male-factor cycles, Percoll extracted a higher total number of spermatozoa (P = 0.02), increased the concentration of motile spermatozoa (P = 0.02), increased the total number of motile spermatozoa per sample (P = 0.02), and enhanced the recovery rate of motile spermatozoa (P = 0.04) compared to swim-up. There was a significant improvement in fertilization rates (P = 0.0006), in the percentage of embryos over 2-cell stage on day of transfer (P = 0.004), and in the number of replaced embryos per transfer (P = 0.01) in the Percoll as compared to swim-up cycles. There was no significant difference in embryo quality. We conclude, therefore, that in advanced reproductive procedures where sperm dysfunction exists, semen preparation with Percoll should replace the swim-up technique.
authors.affiliation: Department of Obstetrics and Gynaecology, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
authors.forename: K A
authors.initials: KA
authors.lastname: Jaroudi
authors.forename: J A
authors.initials: JA
authors.lastname: Carver-Ward
authors.forename: C J
authors.initials: CJ
authors.lastname: Hamilton
authors.forename: U V
authors.initials: UV
authors.lastname: Sieck
authors.forename: K V
authors.initials: KV
authors.lastname: Sheth
mesh_terms: D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D008297:Male; D009865:Oocytes; D011205:Povidone; D012661:Semen / Q000166:cytology*; D012822:Silicon Dioxide; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D016896:Treatment Outcome
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone