Analysis of CA(2+)-binding S100 proteins in human heart by HPLC-electrospray mass spectrometry.
Calcium dependent processes are often impaired in myocardial dysfunctions and calcium-binding proteins might be involved as mediators of Ca2+ signals. Here we present a method to assess a footprint of the calcium-binding proteins of the S100 family in human heart. The S100 proteins are purified through calcium dependent affinity chromatography and reverse phase high-performance liquid chromatography and are analyzed by electrospray-ionization mass spectrometry (ESI-MS) and liquid secondary ionization/tandem quadrupole mass spectrometry (LS-MS/MS). In human heart we identified S100 alpha, CACY, and CAPL as the most abundant S100 proteins and showed that they occur as monomers and homodimers.
Pedrocchi
M
M
Department of Pediatrics, University of Zürich, Switzerland.
Hauer
C R
CR
Schäfer
B W
BW
Erne
P
P
Heizmann
C W
CW
D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000737:chemistry; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D046911:Macromolecular Substances; D013058:Mass Spectrometry; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D046911:Macromolecular Substances