Effect of glucose and deoxyglucose on cytoplasmic [Ca2+] in Ehrlich ascites tumor cells.
Concentration of free cytoplasmic Ca2+ ([Ca2+]i) in Ehrlich ascites tumor cells, measured using fura-2, amounted to 170-300 nM and was increased by 50-160 nM after addition of 10 mM D-glucose or D-2-deoxyglucose but not 3-O-methylglucose at pH 7.4. In the range of external pH between 6.8 and 7.8 the increase was higher at higher pH. This increase occurred within 30-60 s after addition of hexose and lasted for at least 10 min. This [Ca2+]i rise was observed both in presence and virtual absence of Ca2+ in the external medium. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose. The mechanism of [Ca2+] in the external medium. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose. The mechanism of [Ca2+]i rise evoked by glucose and deoxyglucose and its importance in switching cell metabolism from oxidative to glycolytic are discussed.
Teplova
V V
VV
Nencki Institute of Experimental Biology, Warsaw, Poland.
Bogucka
K
K
Czyz
A
A
Evtodienko
Y V
YV
Duszyński
J
J
Wojtczak
L
L
D019325:3-O-Methylglucose; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008757:Methylglucosides / Q000494:pharmacology; D051379:Mice; D013050:Spectrometry, Fluorescence; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
D008757:Methylglucosides; D013729:Terpenes; D019325:3-O-Methylglucose; D019284:Thapsigargin; D003847:Deoxyglucose; D000252:Calcium-Transporting ATPases; D005947:Glucose; D002118:Calcium