Elongation of growth cone filopodia observed with video-enhanced differential interference contrast microscopy.
Dynamics of filopodia configuration from growth cones of neurons isolated from the rat central nervous system were analyzed by using video-enhanced differential interference contrast microscopy. Rapid elongation of filopodia from growth cones and dendrites was observed when high K+ Krebs solution was applied to the growth cones. The maximum speed of the elongation was about 1 micron/s. The filopodia suspended in control Krebs solution moved at random. Retraction of the filopodia was observed within a few minutes after high K+ stimulation.
Tatsumi
H
H
Department of Autonomic Physiology, Tokyo Medical and Dental University, Japan.
Sasaki
H
H
Katayama
Y
Y
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D003712:Dendrites / Q000187:drug effects / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical
D011188:Potassium