Developmentally regulated expression of CGRP in the mouse olfactory pathway.
The pattern of expression of the neuropeptide CGRP and its encoding mRNA has been determined by immunohistochemistry and in situ hybridization in the mouse olfactory pathway during development. Specific CGRP transcripts are first detected at E13 followed by the appearance of the peptide at E15. Both peptide and transcript are present until birth; their expression then appears to be down-regulated since postnatally the peptide is only observed in some olfactory receptor neurons. A monoclonal antibody that specifically recognizes the neurofilament subunit NF-M has been used in order to identify olfactory and trigeminal axons. Our results demonstrate that CGRP is expressed in olfactory neurons and their axons during development, thus supporting further its role as a differentiation factor during olfactory bulb ontogenesis.
Denis-Donini
S
S
CNR Center of Cytopharmacology, Milano, Italy.
Chini
B
B
Vitadello
M
M
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008815:Mice, Inbred Strains; D009833:Olfactory Pathways / Q000196:embryology* / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism
D015347:RNA Probes; D012333:RNA, Messenger; D015740:Calcitonin Gene-Related Peptide