[Human thyroid gland DNA methylase in nodular and diffuse goiter].
The heterogeneity and some properties of DNA-methylases isolated from nonmalignant human thyroid formations--nodular and diffuse goiters--have been studied. Isoelectrofocusing of methylase preparations produced 6-7 distinct activity peaks distinguished by pI, activity towards Ca2+ and Mg2+, sensitivity towards dithiothreitol and capacity to methylase cytosine into mono-, di- and tripyrimidine blocks in vitro. The degree of DNA methylation in vivo depended on the origin of of the goiter. Analysis of several methylase fractions by two-dimensional electrophoresis performed according to O'Farrell revealed the presence of major polypeptides having similar molecular masses (approximately 36 kDa). Besides the major component, all the preparations under study contained 6-10 characteristic minor components. The data obtained are suggestive of a structural and functional heterogeneity of human methylases.
Nikol'skaia
I I
II
Sharkova
E V
EV
Zenina
E N
EN
Lopatina
N G
NG
Atakhanova
B A
BA
Adylova
A T
AT
Koneeva
A E
AE
Kovalev
L I
LI
Shishkin
S S
SS
D015254:DNA Modification Methylases / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006044:Goiter, Nodular / Q000201:enzymology*; D006111:Graves Disease / Q000201:enzymology*; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D013961:Thyroid Gland / Q000201:enzymology*
D015254:DNA Modification Methylases
DNK-metilazy shchitovidnoĭ zhelezy cheloveka pri uzlovom i diffuznom zobe.