Reconstitution of recombinant interleukin-2 (rIL-2): a comparative study of various rIL-2 muteins.
In a previous clinical study using a continuous infusion schedule of recombinant interleukin-2 (rIL-2) we noted a nearly complete loss of activity of EuroCetus rIL-2 when dissolved in 10 ml saline and infused at a very low rate through a plastic infusion device. In the present study, we demonstrated that the loss resulted from a concentration-dependent precipitation of rIL-2 in saline and adherence of the protein to the tubing material. These phenomena were not noted for four other rIL-2 muteins tested [Glaxo, Hoffmann-LaRoche, Amgen (2 muteins)]. EuroCetus rIL-2 was found to be completely soluble in water and 5% glucose.
Vlasveld
L T
LT
Department of Immunology, The Netherlands Cancer Institute, Amsterdam.
Beijnen
J H
JH
Sein
J J
JJ
Rankin
E M
EM
Melief
C J
CJ
Hekman
A
A
D000418:Albumins; D001682:Biological Availability; D005947:Glucose; D007260:Infusion Pumps; D007376:Interleukin-2 / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D011489:Protein Denaturation; D011994:Recombinant Proteins; D012965:Sodium Chloride; D012995:Solubility
D000418:Albumins; D007376:Interleukin-2; D011994:Recombinant Proteins; D012965:Sodium Chloride; D005947:Glucose