title: Separation of rare cell subpopulations with the aid of biotin-labelled ligands.
abstract: A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are first labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10(-4). The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).
authors.affiliation: Department of Pathology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Steinitz
authors.forename: A
authors.initials: A
authors.lastname: Livoff
authors.forename: S
authors.initials: S
authors.lastname: Tamir
authors.forename: T
authors.initials: T
authors.lastname: Brenner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D001710:Biotin; D006801:Humans; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D011956:Receptors, Cell Surface / Q000032:analysis*; D012397:Rosette Formation
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011956:Receptors, Cell Surface; D001710:Biotin