title: Adhesion of parasitized red blood cells to cultured endothelial cells: a flow-based study of isolates from Gambian children with falciparum malaria.
abstract: Adhesion of parasitized red blood cells to vascular endothelium is thought to play an important role in the development of the ischaemic complications associated with severe falciparum malaria. Using a novel, flow-based assay, we have investigated the adhesion of parasitized red blood cells to formalin-fixed human umbilical vein endothelial cells (HUVEC), for isolates obtained from 32 Gambian subjects with mild or severe falciparum malaria. Red cells infected with wild strains of Plasmodium falciparum were able to adhere to HUVEC under physiologically relevant flow conditions, but the level of adhesion was highly variable, ranging from 1 to 688 adherent cells per mm2 of HUVEC. Within isolates, some adherent parasitized cells remained stationary, whilst other formed less stable interactions and rolled slowly over the cell surface. There was no significant difference in adhesion of parasitized cells between isolates obtained from mild or severe cases of malaria, although a subset of isolates did show very high levels of adhesion. The results suggest that there is not a simple relationship between the adhesion of parasitized cells to cultured endothelial cells (presumably via the receptor ICAM-1) and the clinical severity of the disease, although variation in microvascular adhesion in vivo may still be a determinant of ischaemic complications.
authors.affiliation: Department of Haematology, Medical School, University of Birmingham, UK.
authors.forename: B M
authors.initials: BM
authors.lastname: Cooke
authors.forename: S
authors.initials: S
authors.lastname: Morris-Jones
authors.forename: B M
authors.initials: BM
authors.lastname: Greenwood
authors.forename: G B
authors.initials: GB
authors.lastname: Nash
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D004912:Erythrocytes / Q000469:parasitology* / Q000502:physiology; D005260:Female; D017022:Flow Injection Analysis; D005714:Gambia / Q000453:epidemiology; D006801:Humans; D007223:Infant; D007511:Ischemia / Q000209:etiology; D016778:Malaria, Falciparum / Q000097:blood* / Q000453:epidemiology; D008297:Male; D014471:Umbilical Veins / Q000166:cytology