title: Dendritic excitability in CNS neurons: insights from dynamic calcium and sodium imaging in single cells.
abstract: We measured changes in [Ca2+]i and [Na+]i in the dendrites of cerebellar Purkinje cells and hippocampal pyramidal cells using high speed imaging of the fluorescence changes of intracellularly injected fura-2 and SBFI. These transients were detected in synchrony with intracellular recordings of membrane potential. In this way rapid calcium or sodium transients could be associated with specific electrical events. Using this technique we could determine the spatial distribution and source of transients evoked either intrasomatically or synaptically and could relate them to electrically recorded sodium and calcium spikes.
authors.affiliation: Department of Physiology, New York Medical College, Valhalla 10595.
authors.forename: W N
authors.initials: WN
authors.lastname: Ross
authors.forename: H
authors.initials: H
authors.lastname: Miyakawa
authors.forename: V
authors.initials: V
authors.lastname: Lev-Ram
authors.forename: N
authors.initials: N
authors.lastname: Lasser-Ross
authors.forename: J
authors.initials: J
authors.lastname: Lisman
authors.forename: D
authors.initials: D
authors.lastname: Jaffe
authors.forename: D
authors.initials: D
authors.lastname: Johnston
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002490:Central Nervous System / Q000502:physiology* / Q000648:ultrastructure; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D004594:Electrophysiology; D016257:Fura-2; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011689:Purkinje Cells / Q000502:physiology / Q000648:ultrastructure; D017966:Pyramidal Cells / Q000502:physiology / Q000648:ultrastructure; D012964:Sodium / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism
chemical_list: D012964:Sodium; D002118:Calcium; D016257:Fura-2