Characterization of mesophilic Aeromonas from clinical specimens by computerized analysis of SDS-PAGE protein profiles and by enzymatic activity.
Mesophilic Aeromonas (Aeromonas hydrophila, Aeromonas sobria, Aeromonas caviae) have recently been considered important aetiological agents of human diseases, mainly gastrointestinal infections. Although several findings have pointed out the significance of this group of microorganisms as enteric pathogens and suggested the presence of virulence factors, epidemiological and clinical studies are limited by the difficulty of correctly identifying mesophilic Aeromonas at the species level. SDS-PAGE of radiolabelled total protein profiles and bacterial enzymatic activities were used to type 31 clinical isolates (6 A. hydrophila, 7 A. sobria and 18 A. caviae) from patients with gastroenteritis and from healthy controls. Analysis of SDS-PAGE protein patterns, reinforced by the UPGMA-grouping system (AMBIS software) provided a good characterization of A. caviae strains as a homogeneous group of microorganisms, possessing significant differences from the other two species of mesophilic Aeromonas, in good agreement with biochemical and enzymatic tests. Data obtained in analyzing A. sobria protein profiles clearly showed two groups, with a correlation coefficient (CC) = 0.70, which in our experience is a doubtful value for assigning two strains to the same species. Strains biochemically identified as A. hydrophila showed a CC = 0.64, which is equally not acceptable for species assignment. Inter-species comparison highlighted this heterogeneity, showing two mixed subgroups, both containing strains that were assigned to A. sobria and A. hydrophila species on the basis of biochemical features.(ABSTRACT TRUNCATED AT 250 WORDS)
Arzese
A
A
Institute of Microbiology, Medical School, University of Udine, Italy.
Pipan
C
C
Piersimoni
C
C
Scalise
G
G
Morbiducci
V
V
Botta
G A
GA
D000328:Adult; D000333:Aeromonas / Q000737:chemistry / Q000145:classification* / Q000201:enzymology; D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D002648:Child; D001330:Electronic Data Processing; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D008297:Male
D001426:Bacterial Proteins