title: Replication of human papillomavirus (HPV) DNAs supported by the HPV type 18 E1 and E2 proteins.
abstract: Transient replication of human papillomavirus (HPV) type 18 DNA was shown to require the viral E1 and E2 proteins. A 108-bp sequence within the long control region (nucleotides 12 to 119) was sufficient to function as the origin, but maximal replication required a region of 177 bp from positions 7800 to 7857 and 1 to 119 of HPV-18. The E1 and E2 proteins of HPV-18 also supported transient replication of plasmids containing the origins of HPV-1a and bovine papillomavirus type 1 to low levels. Interestingly, the level of replication observed with the HPV-6b origin was higher than that obtained with the homologous HPV-18 origin.
authors.affiliation: Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine 15261.
authors.forename: F
authors.initials: F
authors.lastname: Sverdrup
authors.forename: S A
authors.initials: SA
authors.lastname: Khan
mesh_terms: D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects* / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000494:pharmacology*; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics
chemical_list: D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins; C084762:oncogene protein E1, Human papillomavirus type 18; C069102:oncogene protein E2, Human papillomavirus type 18