Isolation and characterization of a gene for a ryanodine receptor/calcium release channel in Drosophila melanogaster.
The nucleotide sequence of a 25.7 kilobase Drosophila melanogaster genomic DNA segment containing a gene for a ryanodine receptor/calcium release channel homologue has been determined. Computer analysis and partial cDNA cloning revealed 26 exons comprising the protein-coding sequence in this gene. The predicted protein is homologous in amino acid sequence and shares characteristic structural features with the mammalian ryanodine receptors. In blot hybridization analysis, a approximately 16 kilobase RNA species was identified abundantly in a 6-12 h embryo as the transcript from this gene. In situ hybridization to polytene chromosomes indicated that this gene locates at band position 44F on the second chromosome.
Takeshima
H
H
International Institute for Advanced Studies, Kyoto, Japan.
Nishi
M
M
Iwabe
N
N
Miyata
T
T
Hosoya
T
T
Masai
I
I
Hotta
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000737:chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000737:chemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D019837:Ryanodine Receptor Calcium Release Channel
D015220:Calcium Channels; D018076:DNA, Complementary; D009124:Muscle Proteins; D019837:Ryanodine Receptor Calcium Release Channel; D004247:DNA