Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation.
A cDNA clone (Bc329) encoding calmodulin was isolated from a Bryonia cDNA library by screening with cloned Arabidopsis calmodulin cDNA. The cDNA Bc329 was 899 bp full-length clone. The predicted amino acid sequence consists of 149 residues and reveals a high homology with other known plant calmodulins (91 to 99% identity). Genomic southern blot suggests that Bryonia calmodulin is encoded by a single-copy gene. The Bc329 clone was used as a probe to study the expression of calmodulin mRNA after a mechanical stimulus applied on young Bryonia internodes. The steady-state of calmodulin mRNA reached a maximum 30 min after the treatment before it progressively decreased. The role of calcium and calmodulin as second messengers is discussed with regard to environmental changes.
Galaud
J P
JP
U.A. Physiologie Intégree de l'Arbre Fruitier (INRA-Université), Clermont-Ferrand, France.
Lareyre
J J
JJ
Boyer
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D002147:Calmodulin / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013314:Stress, Mechanical; D014675:Vegetables / Q000235:genetics*
D002147:Calmodulin; D012333:RNA, Messenger