title: Theoretical considerations for the application of competitive polymerase chain reaction to the quantitation of a low abundance mRNA: estrogen receptor.
abstract: The necessary theoretical considerations for the development of a correct quantitative analysis of a low abundance messenger RNA (mRNA), estrogen receptor mRNA, by competitive polymerase chain reaction (PCR) are presented together with a series of experimental data. When compared to other methodologies currently utilized for RNA quantitation, this PCR application proved to be a very reliable, rapid and sensitive method. Furthermore, the PCR-based quantitative method described is of particular interest since it does not require the use of radiolabeled compounds.
authors.affiliation: Milano Molecular Pharmacology Laboratory, University of Milan, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Santagati
authors.forename: E
authors.initials: E
authors.lastname: Bettini
authors.forename: M
authors.initials: M
authors.lastname: Asdente
authors.forename: M
authors.initials: M
authors.lastname: Muramatsu
authors.forename: A
authors.initials: A
authors.lastname: Maggi
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D018076:DNA, Complementary / Q000096:biosynthesis; D005260:Female; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011960:Receptors, Estrogen / Q000235:genetics*; D014599:Uterus / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011960:Receptors, Estrogen