Transactivating function of integrated hepatitis B virus.
HBV DNAs are often found in integrated forms in human hepatocellular carcinoma (HCC). Discovery of a transactivation function coded by a limited region of the HBV genome has promoted us to survey our collection of HBV integrants with flanking cellular sequences, asking whether they might exhibit a transactivation function. In transient cotransfection assays using the HepG2 cell line, six out of the twelve integrants showed transactivation effects on the expression of cellular genes such as c-fos. These results strongly demonstrate that the transactivating effects of integrated HBV DNA are widely distributed, and some of these effects might be correlated to hepatocarcinogenesis.
Yamamoto
S
S
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Mita
E
E
Nakatake
H
H
Takimoto
M
M
Koshy
R
R
Matsubara
K
K
D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000382:microbiology; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000378:metabolism; D004273:DNA, Neoplasm / Q000737:chemistry; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000382:microbiology; D015183:Restriction Mapping; D015533:Transcriptional Activation; D014162:Transfection; D014407:Tumor Cells, Cultured; D016662:Virus Integration
D004273:DNA, Neoplasm; D004279:DNA, Viral; D015500:Chloramphenicol O-Acetyltransferase