Discovery of biologically active peptides in random libraries: solution-phase testing after staged orthogonal release from resin beads.
To speed drug discovery, we developed an approach for identification of individual peptides with a desired biological activity from a library containing millions of peptides. The approach uses sequential orthogonal release of chemically synthesized peptides from insoluble beads, followed by testing in solution. In this system, each bead within a library of beads has one peptide sequence, but peptide molecules are attached to the bead with three types of chemical linkers, including two linkers cleavable at different pH optima. An uncleavable linker keeps some peptide attached to the bead for sequencing positives from the solution assay. Applicability of this discovery technique was documented by identifying ligands for a monoclonal antibody and for the human platelet fibrinogen receptor, glycoprotein IIb/IIIa.
Salmon
S E
SE
Arizona Cancer Center, University of Arizona College of Medicine, Tucson 85724.
Lam
K S
KS
Lebl
M
M
Kandola
A
A
Khattri
P S
PS
Wade
S
S
Pátek
M
M
Kocis
P
P
Krchnák
V
V
Thorpe
D
D
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011487:Protein Conformation; D001615:beta-Endorphin / Q000276:immunology
D000911:Antibodies, Monoclonal; D008024:Ligands; D009842:Oligopeptides; D010455:Peptides; D010980:Platelet Membrane Glycoproteins; D001615:beta-Endorphin