Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid.
A polymerase chain reaction (PCR) procedure coupled to an enzyme-linked oligonucleotide sorbent assay (ELOSA; a PCR-ELOSA) identified all 24 serotypic variants of bluetongue virus (BTV) without identifying any of six viruses belonging to the related epizootic hemorrhagic disease virus serogroup. The PCR-ELOSA detected 0.01 50% cell culture infectious doses of each serotype of BTV. The sensitivity and serogroup-wide specificity of the PCR-ELOSA may enable it to replace the more expensive, time-consuming, and biohazardous methods used in the identification of BTV.
Katz
J B
JB
Diagnostic Virology Laboratory, U.S. Department of Agriculture, Ames, Iowa 50010.
Alstad
A D
AD
Gustafson
G A
GA
Moser
K M
KM
D001483:Base Sequence; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003124:Colorimetry; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes