Cryptosporidium parvum infection of intestinal epithelium: morphologic and functional studies in an in vitro model.
A monolayer of mature polarized colonic epithelial cells (T84) able to generate and maintain a barrier to macromolecular flow was used to study pathophysiologic events that occur on microvillus cell exposure to Cryptosporidium parvum. By 24-48 h, several life cycle forms were seen in parasitophorous vacuoles near the apical cell surface, along with a time- and oocyst dose-dependent reduction in epithelial barrier function. As few as 10(5) organisms constituted a successful infecting dose, and heat inactivation of organisms markedly reduced the monolayer barrier alteration. Horseradish peroxidase flux studies demonstrated a substantial increase in macromolecular permeability of the monolayer, and lactate dehydrogenase determinations indicated modest injury of the T84 epithelial cells on exposure to oocysts. Thus, disruption of the epithelial cell barrier, not just opening of transcellular channels for ion flow as reported previously, is responsible for the effects of C. parvum oocysts on intestinal epithelium.
Adams
R B
RB
Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908.
Guerrant
R L
RL
Zu
S
S
Fang
G
G
Roche
J K
JK
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002463:Cell Membrane Permeability; D016785:Cryptosporidium parvum / Q000254:growth & development* / Q000648:ultrastructure; D017097:Electric Impedance; D004594:Electrophysiology; D004848:Epithelium / Q000469:parasitology / Q000473:pathology / Q000503:physiopathology; D006735:Horseradish Peroxidase / Q000378:metabolism; D007422:Intestines / Q000469:parasitology* / Q000473:pathology / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008854:Microscopy, Electron; D013997:Time Factors
D007770:L-Lactate Dehydrogenase; D006735:Horseradish Peroxidase