Phage lambda beta protein, a component of general recombination, is associated with host ribosomal S1 protein.
We have purified phage lambda beta protein produced by a recombinant plasmid carrying bet gene and confirm that it forms a complex with a protein of relative molecular mass 70 kDa. Therefore, beta protein, a component of general genetic recombination, is associated with two functionally diverse complexes; one containing exonuclease and the other 70 kDa protein. Using a number of independent methods, we show that 70 kDa protein is the ribosomal S1 protein of E. coli. Further, the association of 70 kDa protein with beta protein is biologically significant, as the former inhibits joining of the terminal ends of lambda chromosome and renaturation of complementary single stranded DNA promoted by the latter. More importantly, these findings initiate an understanding of an important mode of host- virus interaction in general with specific implication(s) in homologous genetic recombination.
Muniyappa
K
K
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Mythili
E
E
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010582:Bacteriophage lambda / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D008970:Molecular Weight; D009695:Nucleic Acid Renaturation; D010957:Plasmids; D011995:Recombination, Genetic; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D012269:Ribosomal Proteins; D014764:Viral Proteins; C015494:ribosomal protein S1