A micellar liquid chromatographic procedure for the determination of amiloride, bendroflumethiazide, chlorthalidone, spironolactone and triamterene in pharmaceuticals.
A procedure for the determination of amiloride, bendroflumethiazide, chlorthalidone, spironolactone and triamterene in pharmaceutical preparations (tablets) by micellar liquid chromatography, using a 0.07 M SDS-0.5% pentanol mobile phase, is proposed. Recoveries were found in the 100-108% range, with relative standard deviations of 0.4-3.1%. Elution of the most retained diuretics occurred in less than 18 min (at a 1 ml min-1 flow rate). The change in the values of the solute-micelle binding constants and the partition coefficients of the diuretics between the stationary phase and water, upon addition of pentanol, was also studied.
Bonet-Domingo
E
E
Departamento de Química Analítica, Facultad de Química, Universitat de València, Spain.
Medina-Hernandez
M J
MJ
Garcia-Alvarez-Coque
M C
MC
D000584:Amiloride / Q000032:analysis; D001539:Bendroflumethiazide / Q000032:analysis; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002752:Chlorthalidone / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004232:Diuretics / Q000032:analysis*; D008823:Micelles; D013148:Spironolactone / Q000032:analysis; D013607:Tablets; D014223:Triamterene / Q000032:analysis
D004232:Diuretics; D008823:Micelles; D013607:Tablets; D013148:Spironolactone; D001539:Bendroflumethiazide; D000584:Amiloride; D002752:Chlorthalidone; D014223:Triamterene