title: Thermal stability of protein secondary structure in Langmuir-Blodgett films.
abstract: The temperature dependence of the secondary structure of photosynthetic reaction centres from Rhodobacter sphaeroides in solution and in Langmuir-Blodgett film was studied by circular dichroism. It was shown that the secondary structure of the protein was not affected in Langmuir-Blodgett films by heating up to 200 degrees C, while in solution it was completely lost at 55 degrees C. Molecular order rather than decreased hydration degree was held responsible.
authors.affiliation: Institute of Biophysics, University of Genova, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Nicolini
authors.forename: V
authors.initials: V
authors.lastname: Erokhin
authors.forename: F
authors.initials: F
authors.lastname: Antolini
authors.forename: P
authors.initials: P
authors.lastname: Catasti
authors.forename: P
authors.initials: P
authors.lastname: Facci
mesh_terms: D002942:Circular Dichroism; D006358:Hot Temperature; D017433:Protein Structure, Secondary; D012242:Rhodobacter sphaeroides / Q000737:chemistry; D012996:Solutions; D013816:Thermodynamics
chemical_list: D012996:Solutions