Changes in in vivo protein-DNA interactions occur at the c-myc P2 promoter during differentiation.
Down regulation of the c-myc gene is a prerequisite for the differentiation of a number of cell types. Studies have shown that two mechanisms of inactivation are involved in c-myc repression: a block of the elongation of RNA polymerase followed by transcriptional inactivation mediated through promoter sequences. In this study DMS in vivo footprinting was performed on the P2 promoter region of c-myc in differentiated and undifferentiated HL60 cells. A differentiation-specific footprint was observed at G residues immediately upstream of the TATA box. This observation occurred only in cells differentiated for 48 hours or more and hence is likely to be involved in the repression of c-myc by promoter inactivation.
Bryans
M
M
Department of Hematology/Oncology, Ohio State University Comprehensive Cancer Center, 1248 James Cancer Hospital and Research Institute, Columbus 43210.
Lang
J C
JC
Wilkie
N M
NM
D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D017931:DNA Primers; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012334:RNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis; D016385:TATA Box; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012334:RNA, Neoplasm; D004121:Dimethyl Sulfoxide