Effect of serum and cell density on transmembrane distribution of cAMP and cGMP in transformed (C4-I) and non-transformed (WI-38) human cells.
The ratio between cGMP and cAMP in plasma/urine is elevated in several types of malignancies. The present in vitro study showed that the ratio between extracellular cGMP and cAMP increased during the proliferation of C4-I cells (derived from a carcinoma of the uterine cervix), whereas this ratio decreased in WI-38 cells (normal lung fibroblasts). These results can be explained by differences between the transformed and non-transformed cells in the cell-density-dependent transmembrane distribution and intracellular levels of cyclic nucleotides. In the serum-deprived cultures, no profound effects were seen on the cell-density-dependent biokinetics of cAMP and cGMP. In the absence of serum, growth of C4-I cells was markedly retarded, whereas WI-38 cells were unable to expand at all.
Orbo
A
A
Department of Gynecologic Oncology, University of Tromsø, Norway.
Jaeger
R
R
Sager
G
G
D002452:Cell Count; D002455:Cell Division; D002461:Cell Line, Transformed; D003470:Culture Media; D016895:Culture Media, Serum-Free; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D005260:Female; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
D003470:Culture Media; D016895:Culture Media, Serum-Free; D000242:Cyclic AMP; D006152:Cyclic GMP