Rapid and quantitative assay system for measuring anti-infectious bursal disease virus antibody using monoclonal antibody bound to polystyrene latex microspheres.
A monoclonal antibody (MAb) to infectious bursal disease virus (IBDV) that has virus-neutralizing activity was bound to polystyrene latex microspheres The microspheres agglutinated with extracts of bursae from chickens infected with IBDV. Agglutination was inhibited in a competitive manner by adding serum obtained from IBDV-infected chickens. The level of agglutination-inhibition depended on the serum antibody titer against IBDV. The reaction was visually accomplished within 5 minutes. The titer of this rapid assay showed a close relationship with that of the virus-neutralization test.
Nakamura
T
T
Nippon Institute for Biological Science, Tokyo, Japan.
Otaki
Y
Y
Lin
Z
Z
Kato
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D001667:Binding, Competitive; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary*; D002645:Chickens; D005069:Evaluation Studies as Topic; D007243:Infectious bursal disease virus / Q000276:immunology*; D007840:Latex; D007841:Latex Fixation Tests / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D009500:Neutralization Tests; D011137:Polystyrenes; D011201:Poultry Diseases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007840:Latex; D011137:Polystyrenes