title: Differential distribution of tau proteins in developing cat cerebellum.
abstract: The differential distribution and phosphorylation of tau proteins in cat cerebellum was studied with two well characterized antibodies, TAU-1 and TAU-2. TAU-1 detects tau proteins in axons, and the epitope in perikarya and dendrites is masked by phosphorylation. TAU-2 detects a phosphorylation-independent epitope on tau proteins. The molecular composition of tau proteins in the range of 45 kD to 64 kD at birth changed after the first postnatal month to a set of several adult variants of higher molecular weights in the range of 59 kD to 95 kD. The appearance of tau proteins in subsets of axons corresponds to the axonal maturation of cerebellar local-circuit neurons in granular and molecular layers and confirms previous studies. Tau proteins were also identified in synapses by immunofluorescent double-staining with synapsin I, located in the pinceau around the Purkinje cells, and in glomeruli. Dephosphorylation of juvenile cerebellar tissue by alkaline phosphatase indicated indirectly the presence of differentially phosphorylated tau forms mainly in juvenile ages. Additional TAU-1 immunoreactivity was unmasked in numerous perikarya and dendrites of stellate cells, and in cell bodies of granule cells. Purkinje cell bodies were stained transiently at juvenile ages. During postnatal development, the intensity of the phosphate-dependent staining decreased, suggesting that phosphorylation of tau proteins in perikarya and dendrites may be essential for early steps in neuronal morphogenesis during cat cerebellum development.
authors.affiliation: Institut d'Anatomie, Université de Lausanne, Switzerland.
authors.forename: B M
authors.initials: BM
authors.lastname: Riederer
authors.forename: L I
authors.initials: LI
authors.lastname: Binder
mesh_terms: D000818:Animals; D002415:Cats; D002531:Cerebellum / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000493:pharmacokinetics; D010766:Phosphorylation; D016875:tau Proteins / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D008869:Microtubule-Associated Proteins; D016875:tau Proteins