Diethylene glycol distearate (DGD): a versatile embedding medium for retinal cytochemistry.
Embedment in diethylene glycol distearate (DGD) was shown to be highly desirable and versatile for retinal cytochemical studies, including in situ hybridization, immuno- and lectin cytochemistry. This method allows for preservation of fine tissue detail as well as good reaction sensitivity. It appears to be more suitable than most other methods currently used for light microscopic retinal cytochemistry.
Huang
J C
JC
Section of Medical Genetics School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
Mieziewska
K
K
Philp
N
N
van Veen
T
T
Aguirre
G D
GD
D000818:Animals; D016375:Antisense Elements (Genetics); D004285:Dogs; D005026:Ethylene Glycols; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006652:Histological Techniques; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D037102:Lectins; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012160:Retina / Q000166:cytology*; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology; D017948:Retinal Rod Photoreceptor Cells / Q000166:cytology; D017299:Rod Opsins / Q000096:biosynthesis; D014158:Transcription, Genetic
D016375:Antisense Elements (Genetics); D005026:Ethylene Glycols; D037102:Lectins; D015347:RNA Probes; D012333:RNA, Messenger; D017299:Rod Opsins; C007375:diethylene glycol distearate