Purification of mouse antibodies and Fab fragments.
Although monoclonal antibodies can have different properties, it should still be possible to purify any antibody to homogeneity. Often, by utilizing these differences, the purification procedure can be improved. All of the methods discussed are fairly simple and fast. With 1 week of work, over 50 mg of purified antibody can be realized. These methods can also yield material of sufficient quality for structural studies.
Smith
T J
TJ
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003460:Crystallization; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice; D010206:Papain; D010434:Pepsin A; D013205:Staphylococcal Protein A
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D010206:Papain; D010434:Pepsin A