The inhibitory actions of protease inhibitors on the production of polymorphonuclear leukocyte elastase and interleukin 8.
The inhibitory actions of Ulinastatin, which is a protease inhibitor, on the production of polymorphonuclear leukocyte elastase (PMN-elastase) and interleukin 8 (IL-8) in vascular endothelial cells were evaluated. Our findings suggest that IL-8 plays a role in the production of PMN-elastase. Ulinastatin inhibited the lipopolysaccharide (LPS)-stimulated activity of polymorphonuclear leukocytes (PMN) and the production of IL-8 in vascular endothelial cells. Ulinastatin also inhibited the LPS-stimulated production of PMN-elastase in PMN.
Endo
S
S
Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Inada
K
K
Yamashita
H
H
Takakuwa
T
T
Nakae
H
H
Yamada
Y
Y
Kasai
T
T
Taki
K
K
Yoshida
M
M
D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D010196:Pancreatic Elastase / Q000096:biosynthesis*; D014361:Trypsin Inhibitors / Q000494:pharmacology*; D014471:Umbilical Veins
D006023:Glycoproteins; D016209:Interleukin-8; D008070:Lipopolysaccharides; D014361:Trypsin Inhibitors; D010196:Pancreatic Elastase; C028665:urinastatin