Early and quantitative detection of cisplatin-induced nephrotoxicity by measurement of plasma PSP (phenolsulfonphthalein) half-life in rats.
Renal function following cisplatin injection in rats was assessed by measuring the half-life of plasma PSP. Changes in BUN, creatinine, plasma PSP half-life and urinary N-acetyl-beta-D-glucosaminidase (NAG) levels were determined in rats treated with 6.5 mg/kg i.v. of cisplatin. The highest values of BUN and creatinine were both observed on day 5. Half-life of plasma PSP was significantly prolonged on days 2 through 5 and reached a peak value on day 3. A significant increase in urinary NAG levels appeared on day 2 and the peak value was demonstrated on day 3. BUN, creatinine and plasma PSP half-life were also determined on days 3 and 5 following several doses of i.v. cisplatin. A dose-related increase in plasma PSP half-life was observed on day 3, while dose-related increases in BUN and creatinine levels were found on day 5. The evaluation of cisplatin-induced nephrotoxicity by the plasma PSP test was clearly superior for early detection compared with conventional renal function tests using BUN and creatinine.
Uozumi
J
J
Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ueda
T
T
Koikawa
Y
Y
Yasumasu
T
T
Kumazawa
J
J
D000818:Animals; D001806:Blood Urea Nitrogen; D002945:Cisplatin / Q000009:adverse effects*; D003404:Creatinine / Q000097:blood; D006207:Half-Life; D007668:Kidney / Q000187:drug effects*; D007677:Kidney Function Tests; D008297:Male; D010637:Phenolsulfonphthalein / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D003404:Creatinine; D010637:Phenolsulfonphthalein; D002945:Cisplatin