Single-copy T-DNA insertions in Arabidopsis are the predominant form of integration in root-derived transgenics, whereas multiple insertions are found in leaf discs.
Different patterns of T-DNA integration in Arabidopsis were obtained that depended on whether a root or a leaf-disc transformation method was used. An examination of 82 individual transgenic Arabidopsis plants, derived from 15 independent Agrobacterium-mediated transformations in which different cointegrate and binary constructs were used, indicated that the transformation method had a significant influence on the type and copy number of T-DNA integration events. Southern hybridizations showed that most of the transgenic plants produced by a leaf-disc method contained multiple T-DNA insertions (89%), the majority of which were organized as right-border inverted repeat structures (58%). In contrast, a root transformation method mostly resulted in single T-DNA insertions (64%), with fewer right-border inverted repeats (38%). The transformation vectors, including cointegrate and binary types, and the plant selectable markers, hygromycin phosphotransferase and dihydrofolate reductase, did not appear to influence the T-DNA integration patterns.
Grevelding
C
C
Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Fantes
V
V
Kemper
E
E
Schell
J
J
Masterson
R
R
D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D017343:Genes, Plant; D005822:Genetic Vectors; D030821:Plants, Genetically Modified / Q000235:genetics*; D011995:Recombination, Genetic; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA