title: Molecular cloning, expression, and pharmacological characterization of humEAA1, a human kainate receptor subunit.
abstract: Kainate is a potent neuroexcitatory agent; its neurotoxicity is thought to be mediated by an ionotropic receptor with a nanomolar affinity for kainate. In this report, we describe the cloning of a cDNA encoding a human glutamate ionotropic receptor subunit protein from a human hippocampal library. This cDNA, termed humEAA1, is most closely related to rat and human cDNAs for kainate receptor proteins and, when expressed in COS or Chinese hamster ovary cells, is associated with high-affinity kainate receptor binding. We have successfully established cell lines stably expressing humEAA1. This is the first report of establishment of stable cell lines expressing a glutamate receptor subunit. The relative potency of compounds for displacing [3H]kainate binding of humEAA1 receptors expressed in these stable cell lines was kainate > quisqualate > domoate > L-glutamate >> (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid > dihydrokainate > 6,7-dinitroquinoxaline-2,3-dione > 6-cyano-7-nitroquinoxaline-2,3-dione. Homooligomeric expression of humEAA1 does not appear to elicit ligand-gated ion channel activity. Nevertheless, the molecular structure and pharmacological characterization of high-affinity kainate binding of the humEAA1 expressed in the stable cell line (ppEAA1-16) suggest that the humEAA1 is a subunit protein of a human kainate receptor complex.
authors.affiliation: Allelix Biopharmaceuticals, Inc., Mississauga, Ontario, Canada.
authors.forename: R K
authors.initials: RK
authors.lastname: Kamboj
authors.forename: D D
authors.initials: DD
authors.lastname: Schoepp
authors.forename: S
authors.initials: S
authors.lastname: Nutt
authors.forename: L
authors.initials: L
authors.lastname: Shekter
authors.forename: B
authors.initials: B
authors.lastname: Korczak
authors.forename: R A
authors.initials: RA
authors.lastname: True
authors.forename: V
authors.initials: V
authors.lastname: Rampersad
authors.forename: D M
authors.initials: DM
authors.lastname: Zimmerman
authors.forename: M A
authors.initials: MA
authors.lastname: Wosnick
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002645:Chickens; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D007608:Kainic Acid / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D018092:Kainic Acid Receptors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007608:Kainic Acid; D046911:Macromolecular Substances; D018092:Kainic Acid Receptors; C514872:GRIK4 protein, human