title: The occurrence of mitochondrial variations including large mitochondria in osteoclasts following calcitonin treatment.
abstract: Calcitonin-treated osteoclasts with mitochondrial variations, including large mitochondria, and osteoclasts not treated with calcitonin (control osteoclasts) were examined using cytochrome c oxidase activity and morphological analysis of mitochondria. The calcitonin-treated osteoclasts were divided into two types. One was osteoclasts with only a clear zone, which contained many type 1 mitochondria with the reaction products of cytochrome c oxidase in both the inner membrane of the cristae and the outer compartment in the vascular side and a comparatively small number of type 1 mitochondria in the resorptive side. The other type was osteoclasts with a mature ruffled border, in which many type 1 mitochondria were observed in both sides. Most of the large mitochondria were type 1, and these were observed only in the vascular side of the osteoclasts with only a clear zone, and in both sides of the osteoclasts with a mature ruffled border. In statistical analysis, the cross-sectional area of mitochondria in the vascular side of calcitonin-treated osteoclasts was larger than that of those in the resorptive side, and the numerical percentage of type 1 mitochondria in the vascular side was greater than that in the resorptive side. In contrast, the cross-sectional area of mitochondria in the vascular side of control osteoclasts was smaller than that in the resorptive side, and there was no significant difference between the two groups in the numerical percentage of type 1 mitochondria.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Orthodontics, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Noda
authors.forename: Y
authors.initials: Y
authors.lastname: Kuwahara
mesh_terms: D000818:Animals; D001862:Bone Resorption; D002116:Calcitonin / Q000494:pharmacology*; D003576:Electron Transport Complex IV / Q000096:biosynthesis; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008963:Molar / Q000166:cytology; D010010:Osteoclasts / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002116:Calcitonin; D003576:Electron Transport Complex IV