title: Cloning and characterization of the human t(3;6)(p14;p11) translocation breakpoint associated with hematologic malignancies.
abstract: The t(3;6)(p14;p11) chromosome translocation was identified in a family in which three members developed hematologic malignancies. To help characterize the region on chromosome 3 surrounding this translocation breakpoint, two flanking lambda clones, MS156 and MJ1525, were linked by pulsed-field gel electrophoresis to the same 510-kb NotI fragment on chromosome 3. MS156 was localized to a region proximal to the breakpoint of a der(3) chromosome somatic cell hybrid (derived from the t(3;6) cell line), and MJ1525 localized distal to the breakpoint. MJ1525 was used to screen the CEPH yeast artificial chromosome (YAC) library, which revealed a YAC, 195F3, that spanned the breakpoint. Subcloning into Lambda DASH II and production of a contiguous array of overlapping lambda clones revealed a clone, L17, that spanned the breakpoint. A rare restriction endonuclease map for the YAC 195F3 was constructed, and multiple clusters of rare restriction sites within the YAC were identified, possibly indicating the disruption of a gene by the t(3;6) translocation breakpoint.
authors.affiliation: Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI 48201.
authors.forename: S E
authors.initials: SE
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Joseph
authors.forename: S
authors.initials: S
authors.lastname: Nadeau
authors.forename: V
authors.initials: V
authors.lastname: Shridhar
authors.forename: R
authors.initials: R
authors.lastname: Gemmill
authors.forename: H
authors.initials: H
authors.lastname: Drabkin
authors.forename: S
authors.initials: S
authors.lastname: Knuutila
authors.forename: D I
authors.initials: DI
authors.lastname: Smith
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D018244:Chromosomes, Artificial, Yeast; D002893:Chromosomes, Human, Pair 3; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D006822:Hybrid Cells; D007938:Leukemia / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic