Cloning and characterization of the tandemly arranged bovine lymphotoxin and tumour necrosis factor-alpha genes.
The screening of a bovine genomic library with a human tumour necrosis factor-alpha (TNF-alpha) cDNA probe resulted in the isolation of a 7.2 kb DNA fragment containing the entire bovine TNF-alpha gene. Analysis of this genomic clone showed that it also contains the bovine lymphotoxin (LT, TNF-beta) gene. Comparison to published sequences of human, murine, ovine and rabbit counterparts allowed us to delineate the coding sequences, the promoters and the enhancers of these two genes. Sequences involved in the regulation of translation and in the mRNA stability were found in the 3' untranslated regions.
Cludts
I
I
Department of Molecular Biology, University of Brussels (ULB), Belgium.
Cleuter
Y
Y
Kettmann
R
R
Burny
A
A
Droogmans
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D015698:Genomic Library; D015186:Hominidae / Q000235:genetics; D006801:Humans; D008233:Lymphotoxin-alpha / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics*
D015342:DNA Probes; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; D004247:DNA