Effect of troponin T-treatment on the composition of rabbit skeletal myofibrils.
The effect of several conditions of troponin T-treatment was examined on the SDS-gel electrophoretic patterns of rabbit skeletal myofibrils. More troponin C and I were removed from the myofibrils after troponin T-treatment at acidic pH (5.6-6.2) than after treatment at neutral pH (6.8-7.4). Incubation of the myofibrils (0.4 mg/ml) in the presence of 0.043 mg/ml troponin T at a pH 6.2 for 60 min at 25 degrees C resulted in the removal of most troponin C and I from the myofibrils. Troponin T1 (N-terminal 158 residue fragment of troponin T of 259 residues) showed much lower troponin C.I removing activity than troponin T.
Shiraishi
F
F
Department of Pharmacology, Faculty of Medicine, Kyusyu University, Fukuoka.
Ohtsuki
I
I
Yamamoto
K
K
D000818:Animals; D003470:Culture Media; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009210:Myofibrils / Q000737:chemistry*; D011817:Rabbits; D014336:Troponin / Q000032:analysis / Q000494:pharmacology*; D019209:Troponin C; D019210:Troponin I; D020107:Troponin T
D003470:Culture Media; D014336:Troponin; D019209:Troponin C; D019210:Troponin I; D020107:Troponin T