title: A simplified method for quantification of myocardial blood flow using nitrogen-13-ammonia and dynamic PET.
abstract: The utility of Patlak graphical analysis was investigated for quantification of regional myocardial blood flow (MBF) and for generating parametric images of MBF with 13N-ammonia and dynamic PET imaging in dogs and humans. MBF was estimated by a two-compartment model fit of the initial 2 min of the kinetic data and by Patlak graphical analysis of the initial 2, 3, or 4 min of data. In 11 dog studies, MBF by compartmental model fitting linearly correlated with MBF by microspheres (correlation coefficient (r) = 0.99, slope = 0.92) and by Patlak graphical analysis (r = 0.99, slope = 0.90). In 10 normal human studies, MBF obtained by the Patlak graphical analysis agreed well with MBF obtained by the compartmental model fitting (r = 0.96, slope = 1.04). Good agreement of the MBF estimates was also observed in 10 coronary artery disease patient studies (r = 0.96). Patlak graphical analysis permitted generation of parametric images of MBF. The parametric images of MBF, in units of ml/min/g, are of good image quality and have relatively low noise levels. We conclude that regional MBF can be noninvasively and conveniently measured with dynamic 13N-ammonia PET using either a two-compartment model or Patlak graphical analysis. MBF parametric images generated with the Patlak graphical analysis both map the distribution and quantitate the magnitude of myocardial perfusion abnormalities.
authors.affiliation: Department of Radiological Sciences, UCLA School of Medicine 90024-1721.
authors.forename: Y
authors.initials: Y
authors.lastname: Choi
authors.forename: S C
authors.initials: SC
authors.lastname: Huang
authors.forename: R A
authors.initials: RA
authors.lastname: Hawkins
authors.forename: W G
authors.initials: WG
authors.lastname: Kuhle
authors.forename: M
authors.initials: M
authors.lastname: Dahlbom
authors.forename: C K
authors.initials: CK
authors.lastname: Hoh
authors.forename: J
authors.initials: J
authors.lastname: Czernin
authors.forename: M E
authors.initials: ME
authors.lastname: Phelps
authors.forename: H R
authors.initials: HR
authors.lastname: Schelbert
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000641:Ammonia; D000818:Animals; D003326:Coronary Circulation; D004285:Dogs; D005260:Female; D006321:Heart / Q000000981:diagnostic imaging; D006801:Humans; D008297:Male; D008875:Middle Aged; D009590:Nitrogen Radioisotopes; D014055:Tomography, Emission-Computed
chemical_list: D009590:Nitrogen Radioisotopes; D000641:Ammonia