Photodynamic elimination of clonogenic Ph+ chronic myeloid leukemia cells.
Benzoporphyrin derivative (BPD) and light is a potent photosensitizer. We investigated this modality as a means to selectively eliminate clonogenic Ph(+) chronic myeloid leukemia (CML) cells. BPD at 10 ng/ml and 10.8 J/cm2 broad spectrum light eliminates from 5 to 6 logs of Ph(+) EM-2 cells. Long-term marrow culture studies of treated mixtures of normal and CML cells indicate that multipotent progenitor cell viability is retained while cells transcribing BCR-ABL are not detected. We conclude that BPD and light may offer a means of providing CML autografts potentially free of Ph(+) clonogenic cells.
Keating
A
A
Toronto Hospital, General Division, Ontario, Canada.
Jamieson
C
C
Hornby
A
A
Wang
X H
XH
Levy
J
J
D000328:Adult; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D002999:Clone Cells / Q000187:drug effects / Q000528:radiation effects; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000473:pathology*; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D010778:Photochemotherapy; D016133:Polymerase Chain Reaction; D011166:Porphyrins / Q000494:pharmacology*; D011836:Radiation Tolerance; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D014410:Tumor Stem Cell Assay
D014408:Biomarkers, Tumor; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C504224:benzoporphyrin D; D016044:Fusion Proteins, bcr-abl