Efficient gene transfer with less cytotoxicity by means of cationic multilamellar liposomes.
A simple procedure for the preparation of cationic multilamellar vesicles (MLV) consisting of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine in a molar ratio of 1:2:2 was devised. When bacteriophage lambda DNA was encapsulated into these liposomes, entrapment efficiency was found to be nearly 100%, and digestibility of the DNA was less than 10%. Upon encapsulation of the plasmid pCH110 into cationic MLV, efficient expression was comparable to that obtained with cationic vesicles prepared by reverse-phase evaporation method (REV). Cytotoxicity of the present liposomes was less than that of cationic REV and far less than that of Lipofectin.
Yagi
K
K
Institute of Applied Biochemistry, Yagi Memorial Park, Mitake, Gifu, Japan.
Noda
H
H
Kurono
M
M
Ohishi
N
N
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D002460:Cell Line; D002470:Cell Survival; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000008:administration & dosage / Q000378:metabolism*; D004337:Drug Carriers; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D005971:Glutamates; D008081:Liposomes; D010430:Pentosyltransferases / Q000096:biosynthesis* / Q000235:genetics; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010957:Plasmids
D004279:DNA, Viral; D004337:Drug Carriers; D005971:Glutamates; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C084512:didodecyl N-(alpha-trimethylammonioacetyl)glutamate; C015153:1,2-dilauroylphosphatidylcholine; C020888:1,2-dielaidoylphosphatidylethanolamine; D010430:Pentosyltransferases; C024827:xanthine phosphoribosyltransferase