Electron microscopy of human erythrocyte catalase: new two-dimensional crystal forms.
Using the mica-spreading "negative staining-carbon film" procedure, human erythrocyte catalase has been shown to create a number of different periodic or crystalline two-dimensional (2-D) arrays which differ in the arrangement of molecules in the repeating units and the lattice type. Digital image processing has been performed with a 2-D array which contains regularly arranged "undulating" rows of molecules and also with a 2-D crystal form, exhibiting pgg (p22(1)2(1)) symmetry and lattice parameters of a = 12.7 nm, b = 44 nm, and gamma = 92 degrees. The data are compared with our previous analysis of a different human erythrocyte catalase 2-D crystal, and the effect of partial-depth negative staining occurring in all the various 2-D forms is discussed.
Harris
J R
JR
Institute of Zoology II, University of Mainz, Germany.
Engelhardt
H
H
Volker
S
S
Holzenburg
A
A
D002374:Catalase / Q000097:blood / Q000648:ultrastructure*; D003198:Computer Simulation; D004912:Erythrocytes / Q000201:enzymology*; D005583:Fourier Analysis; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D008958:Models, Molecular; D011487:Protein Conformation
D002374:Catalase