title: Evaluation of the skeletal effects of combined mild dietary calcium restriction and ovariectomy in Sinclair S-1 minipigs: a pilot study.
abstract: A pilot study was conducted to investigate the combined effects of ovariectomy (OVX) with preceding and concomitant mild dietary calcium restriction on the minipig skeleton. Minipigs 4 months old were fed diets containing 0.9, 0.75, or 0.5% calcium (Ca). At 10 months, the 0.75 and 0.5% pigs were OVX and the 0.9% were either sham operated or OVX. All pigs were maintained on their respective diets for an additional 6 months. Excised lumbar vertebrae and long bones were evaluated by densitometry and histomorphometry, and vertebral cancellous bone samples were tested biomechanically. In pigs fed the 0.9% Ca diet, OVX alone effected decreases of 6% in vertebral bone mineral density (BMD), 15% in trabecular bone volume (BV/TV), and 13% in trabecular number (Tb.N), an increase of 15% in trabecular separation (Tb.Sp), and a nonsignificant increase (p < 0.056) in vertebral cancellous final erosion depth (F.E.De) compared with the 0.9% Ca sham-operated group. Decreasing dietary Ca to 0.5% in combination with OVX effected an 8% reduction in vertebral BMD that was not associated with any significant alterations in parameters of vertebral cancellous bone microstructure or remodeling compared with the 0.9% Ca sham-operated pigs. Increases in serum PTH noted in the 0.5% Ca OVX group were generally paralleled by increases in calcitriol. In OVX pigs fed a diet containing 0.75% Ca, a 10% reduction in vertebral BMD was observed. This was associated with significant increases in F.E.De and vertebral marrow star volume (Ma.St.V) compared with the 0.9% Ca sham-operated pigs and the other OVX groups. In addition, Tb.Sp was increased and Tb.N decreased compared with the 0.9% Ca sham-operated pigs. Increases in serum PTH in this group were not accompanied by increases in calcitriol. Midradial and midfemoral BMD values were reduced in the 0.75 and 0.5% Ca OVX groups compared with the 0.9% Ca sham-operated pigs. Histomorphometric analyses of cortical bone suggested the reduction in cortical bone mass in the 0.75% Ca OVX group may have been largely due to net loss on the endocortical surface versus possible failure to accrue bone in the 0.5% Ca OVX group. Ash density and biomechanical parameters for vertebral cancellous bone decreased progressively in the 0.9% sham-operated, 0.9% Ca OVX, and 0.75% Ca OVX groups and then increased in the 0.5% Ca OVX group. After normalization for bone mass (ash), mechanical changes were still apparent, particularly for the 0.75% Ca OVX group compared with other OVX groups, reflecting that structural changes had taken place in the trabecular network.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Connective Tissue Biology, University of Aarhus, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Mosekilde
authors.forename: S E
authors.initials: SE
authors.lastname: Weisbrode
authors.forename: J A
authors.initials: JA
authors.lastname: Safron
authors.forename: H F
authors.initials: HF
authors.lastname: Stills
authors.forename: M L
authors.initials: ML
authors.lastname: Jankowsky
authors.forename: D C
authors.initials: DC
authors.lastname: Ebert
authors.forename: C C
authors.initials: CC
authors.lastname: Danielsen
authors.forename: C H
authors.initials: CH
authors.lastname: Søgaard
authors.forename: A F
authors.initials: AF
authors.lastname: Franks
authors.forename: M L
authors.initials: ML
authors.lastname: Stevens
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D000818:Animals; D015519:Bone Density; D016723:Bone Remodeling / Q000502:physiology*; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000502:physiology; D002117:Calcitriol / Q000097:blood; D002136:Calcium, Dietary / Q000008:administration & dosage*; D004958:Estradiol / Q000097:blood; D005260:Female; D006728:Hormones / Q000097:blood*; D008954:Models, Biological; D010052:Ovariectomy; D010281:Parathyroid Hormone / Q000097:blood; D010865:Pilot Projects; D013552:Swine; D013556:Swine, Miniature; D016474:Weight-Bearing / Q000502:physiology*
chemical_list: D002136:Calcium, Dietary; D006728:Hormones; D010281:Parathyroid Hormone; D004958:Estradiol; D000135:Acid Phosphatase; D002117:Calcitriol