Studies on the modulating effects of retinoic acid and retinol acetate using dye transfer and metabolic cooperation assays.
The effects of retinoic acid and retinol acetate on gap junctional communication were examined in two in vitro tests. Rat liver epithelial cell line IAR 203 was used for dye transfer assays, and hamster lung fibroblast V79 cells were used for metabolic cooperation assays. A reversible dose-dependent inhibition of dye transfer was detected after a 1-hr treatment with retinoic acid or retinol acetate at concentrations ranging from 10 to 50 microM. On the other hand, enhancement of dye transfer was observed after a 24-hr treatment with retinoic acid at 0.1 microM. A dose-dependent inhibition of metabolic cooperation was obtained with retinoic acid at noncytotoxic concentrations ranging from 5 to 50 microM. Retinoids and TPA (1 ng/ml) acted synergistically in their inhibition of cell communication. Thus, the assays appear to be complementary: the dye transfer assay was useful in studying the time course and the reversibility of the inhibition or enhancement of dye transfer, whereas the metabolic cooperation assay was effective in quantifying the inhibitory effect of TPA or retinoids and interactions between them.
Mercier
T
T
Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Jouy-en-Josas, France.
Honikman-Leban
E
E
Chaumontet
C
C
Martel
P
P
Shahin
M M
MM
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects*; D002452:Cell Count / Q000187:drug effects; D002460:Cell Line; D004396:Coloring Agents / Q000493:pharmacokinetics*; D006224:Cricetinae; D004224:Diterpenes; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017629:Gap Junctions / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D000084562:Retinyl Esters; D013234:Stem Cells / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology*; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology
D004396:Coloring Agents; D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C009166:retinol acetate; D014212:Tretinoin