Comparative study on the expression of stress-response protein (srp) 72, srp 27, alpha B-crystallin and ubiquitin in brain tumours. An immunohistochemical investigation.
This immunohistochemical study compares the expression of stress-response (heat-shock) protein (srp) 72, srp 27, alpha B-crystallin and ubiquitin in 86 primary human brain tumours and 21 carcinoma metastases to the central nervous system. Normal brain tissues were included for control purposes. Serial sections of formalin-fixed, paraffin-embedded tissues were used. Most meningiomas (17/23), glioblastomas (11/12) and breast carcinoma metastases (9/10) and some astrocytomas (7/13), pituitary tumours (4/9) and lung cancer metastases (5/11) had tumour cells that reacted with one or more of the antibodies used. Around 43% of the meningiomas and 25% of the glioblastomas expressed srp 72 only. Sole expression of srp 27, alpha B-crystallin or ubiquitin was seen in several tumours. Some meningiomas (3/23) and breast cancer metastases (4/10) co-expressed srp 72 and srp 27, and 1/3 of the glioblastomas co-expressed srp 27 and alpha B-crystallin. We conclude that primary and metastatic tumours of the brain produce stress-related proteins and that certain tumours concurrently express two or more srp's.
Kato
S
S
Division of Neuropathology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York.
Hirano
A
A
Kato
M
M
Herz
F
F
Ohama
E
E
D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D001943:Breast Neoplasms / Q000473:pathology; D003459:Crystallins / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000473:pathology; D008579:Meningioma / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D018271:Signal Recognition Particle / Q000096:biosynthesis* / Q000235:genetics; D014452:Ubiquitins / Q000096:biosynthesis* / Q000235:genetics
D003459:Crystallins; D006360:Heat-Shock Proteins; C048742:LOC377776 protein, human; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C503636:SRP72 protein, human; D018271:Signal Recognition Particle; D014452:Ubiquitins