title: Molecular cloning of a putative homolog of proline/arginine-rich antibacterial peptides from porcine bone marrow.
abstract: Screening of a porcine bone marrow cDNA library with a PCR-derived probe from rabbit LPS-binding protein CAP18 led to the discovery of two closely related clones. The longer, full-length cDNA clone encodes a 228 amino acid residue protein similar to the family of antibacterial/LPS-binding cationic peptides. In contrast to other hitherto discovered precursors of Pro/Arg-rich peptides from this family, they have a novel, unique structure of the C-terminal region of 100 amino acid residues with a repeating sequence of ten residues (FPPPNXPGPR, where X = V or F). These precursors could represent a part of the antibacterial peptide repertoire of porcine bone marrow.
authors.affiliation: Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Ljubljana, Slovenia.
authors.forename: J
authors.initials: J
authors.lastname: Pungercar
authors.forename: B
authors.initials: B
authors.lastname: Strukelj
authors.forename: G
authors.initials: G
authors.lastname: Kopitar
authors.forename: M
authors.initials: M
authors.lastname: Renko
authors.forename: B
authors.initials: B
authors.lastname: Lenarcic
authors.forename: F
authors.initials: F
authors.lastname: Gubensek
authors.forename: V
authors.initials: V
authors.lastname: Turk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001120:Arginine / Q000032:analysis*; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D011392:Proline / Q000032:analysis*; D055232:Proline-Rich Protein Domains; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013552:Swine; D054804:Cathelicidins
chemical_list: D000890:Anti-Infective Agents; D001120:Arginine; D018076:DNA, Complementary; D010455:Peptides; D011392:Proline; D011498:Protein Precursors; C084994:NPG4 protein, Sus scrofa; D054804:Cathelicidins