Native and baculovirus-expressed forms of the immuno-protective protein BM86 from Boophilus microplus are anchored to the cell membrane by a glycosyl-phosphatidyl inositol linkage.
A glycoprotein (BM86) from the gut cells of the cattle tick Boophilus microplus, when used to vaccinate cattle, has been shown to protect cattle from tick infestation. A recombinant BM86 protein is the principal component of a novel tick vaccine currently under development. The nature of the anchorage of BM86 to tick gut epithelial cells has been investigated using BM86 from B. microplus and recombinant BM86 proteins expressed in insect cells using the baculovirus expression system. BM86 from B. microplus and a full length recombinant BM86 are shown to be anchored to the extracellular surface of tick gut epithelial cells and baculovirus-infected insect cells, respectively by a glycosyl-phosphatidyl inositol membrane anchor. A recombinant BM86 truncated by the removal of a hydrophobic region coding for thirty amino acids at the carboxy-terminal end was secreted from baculovirus-infected Sf9 cells. This secreted form of recombinant BM86 showed strong protective activity against ticks in cattle vaccinated with this protein.
Richardson
M A
MA
Biotech Australia Pty Ltd, Roseville, New South Wales, Australia.
Smith
D R
DR
Kemp
D H
DH
Tellam
R L
RL
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry*; D002478:Cells, Cultured; D003429:Cross Reactions; D004252:DNA Mutational Analysis; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000302:isolation & purification* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D009036:Moths / Q000166:cytology; D010270:Parasite Egg Count; D011994:Recombinant Proteins / Q000096:biosynthesis; D017384:Sequence Deletion; D013379:Substrate Specificity; D013984:Tick Infestations / Q000517:prevention & control*; D013987:Ticks / Q000737:chemistry*; D010738:Type C Phospholipases / Q000378:metabolism; D014612:Vaccines; D014614:Vaccines, Synthetic / Q000627:therapeutic use*
C085065:BM86 protein, Boophilus; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic; D010738:Type C Phospholipases