Evaluation of the capability of different chromatographic systems for the monitoring of thimerosal and its degradation products by high-performance liquid chromatography with amperometric detection.
Several liquid chromatographic systems using electrochemical detection on carbon electrodes were compared for the analysis of thimerosal and its degradation products, thiosalicylic acid and dithiodibenzoic acid. The studied separation systems included reversed-phase ion-suppressed chromatography, reversed-phase ion-pair chromatography and ion chromatography. Amperometry and coulometry were evaluated as electrochemical detection techniques. The best method for thimerosal determination in ophthalmic solutions in terms of selectivity and sensitivity was ion-pair chromatography.
del Pilar da Silva
M
M
Department of analytical Chemistry and Instrumental Analysis, Science Faculty, Autonoma University of Madrid, Spain.
Procopio
J R
JR
Hernández
L
L
D001565:Benzoates / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D004563:Electrochemistry; D009883:Ophthalmic Solutions / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds; D013849:Thimerosal / Q000032:analysis* / Q000737:chemistry
D001565:Benzoates; D009883:Ophthalmic Solutions; D013438:Sulfhydryl Compounds; C026196:2-thiosalicylic acid; D013849:Thimerosal