Sodium extrusion mechanism in mammalian cochlear outer hair cell.
Intracellular sodium concentration ([Na+]i) in isolated outer hair cells (OHCs) of guinea pig cochlea was measured by microfluorimetric method using sodium binding benzofuran isophthalate. An application of ouabain increased [Na+]i. Our results suggest that OHCs have the definite activity of Na+/K+ ATPase.
Sunose
H
H
Department of Otolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Ikeda
K
K
Saito
Y
Y
Nishiyama
A
A
Takasaka
T
T
D000818:Animals; D003051:Cochlea / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D005456:Fluorescent Dyes; D005470:Fluorometry; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D005456:Fluorescent Dyes; D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium