Expression of DcPRP1 is linked to carrot storage root formation and is induced by wounding and auxin treatment.
A carrot (Daucus carota, L.) genomic clone (DcPRP1) was isolated on the basis of its homology to previously described cDNAs encoding a wound-inducible, proline-rich cell wall protein. DNA sequence analysis showed that DcPRP1 contains a single open-reading frame encoding a 235-amino acid protein that is colinear with that predicted from the cDNA sequence with the exception of four amino acids at the N terminus and a 60-nucleotide insertion present within the genomic clone. Genomic Southern hybridization analysis showed that the cloned sequence hybridized with a single restriction enzyme fragment using several restriction enzymes. Primer extension and northern hybridization analysis indicated that the expression of DcPRP1 is developmentally regulated and linked to the formation of storage roots, where this gene is expressed at high levels after wounding. The level of DcPRP1 mRNA was greatest in tissue immediately adjacent to the wound site. Treatment of unwounded carrot storage roots with 10 microM 2,4-dichlorophenoxy-acetic acid, indoleacetic acid, or naphthalene-1-acetic acid also resulted in the accumulation of DcPRP1 transcripts to a level equal to that seen in wounded tissue.
Ebener
W
W
Agronomy Department, Ohio Agricultural Research and Development Center, Ohio State University, Columbus 43210.
Fowler
T J
TJ
Suzuki
H
H
Shaver
J
J
Tierney
M L
ML
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D015698:Genomic Library; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D055232:Proline-Rich Protein Domains; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution; D014675:Vegetables / Q000187:drug effects / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D007210:Indoleacetic Acids; D010455:Peptides; D010940:Plant Proteins; D012333:RNA, Messenger