[DNA typing of the three HLA-class II loci from saliva stains].
The DNA typing of the HLA-class II loci, HLA-DPB1, HLA-DQA1 and HLA-DRB, from saliva stains is described. In addition to the experimentally prepared saliva stains, a stain on a towel in a burglar case was subjected to the analysis. Using 10 ng of the extracted DNA, we could type all the three loci with all of the specimens tested. The incidence of the saliva stain in the practical case was estimated to be 3.18 x 10(-5) in Japanese population. We found that the aging of saliva stains at least six months did not significantly influence the DNA typing.
Kojima
T
T
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
Uchihi
R
R
Yamamoto
T
T
Tamaki
K
K
Katsumata
Y
Y
D004247:DNA / Q000235:genetics*; D005554:Forensic Medicine; D005802:Genes, MHC Class II; D006682:HLA-DP Antigens / Q000235:genetics; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry*
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D006684:HLA-DR Antigens; D004247:DNA