An assay for the detection of bacterial DNA gyrase inhibitors.
In summary, we have developed a sensitive detection system for inhibitors of bacterial DNA gyrase. The use of B. subtilis as the host organism confers the advantage that it is sensitive to both gyrase subunit A and B inhibitors, whereas E. coli is relatively insensitive to B subunit inhibitors in vivo. Using this assay, we identified a new DNA gyrase inhibitor with a novel structure.
Osburne
M S
MS
American Cyanamid Company, Lederle Laboratories, Department of Microbial Genetics and Biochemistry, Pearl River, NY 10965.
Maiese
W M
WM
Greenstein
M
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects*; D004269:DNA, Bacterial / Q000187:drug effects*; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D059005:Topoisomerase II Inhibitors; D001616:beta-Galactosidase / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D059005:Topoisomerase II Inhibitors; D001616:beta-Galactosidase