Characterization of the Neisseria Iga beta-core. The essential unit for outer membrane targeting and extracellular protein secretion.
Extracellular transport of Neisseria IgA proteases across the bacterial outer membrane is accomplished by the translocation function contained within the C-terminal Iga beta domain of IgA protease precursor proteins. Recently, we reported that Iga beta from N. gonorrhoeae MS11 (Val1097 to Phe1505), fused to a periplasmic passenger protein, facilitated its transport across the outer membrane, leading to surface exposure of the passenger. In the present work we show, by systematic N-terminal truncation of Iga beta, that the functional and structural unit, termed Iga beta-core, corresponds to the C-terminal approximately 274 amino acid residues (Ser1231 to Phe1505). This minimal region retains all the essential features necessary for the translocation of an N-terminally attached passenger across the outer membrane of Escherichia coli, and for its own correct integration into the outer membrane, even in the absence of a passenger protein. The membrane-integrated Iga beta-core constitutes a conserved entity found in the C-terminal regions of Iga beta domains of different N. gonorrhoeae, N. meningitidis and Haemophilus influenzae strains. In contrast, the surface-exposed N termini of the Iga beta domains vary in size and sequence. Based on secondary structure predictions, the key structural feature of the core is a beta-barrel (amphipathic, antiparallel transmembrane beta-strands, interspersed by hairpin turns and loops) which is common to many integral outer membrane proteins of Gram-negative bacteria. We propose that the core has been conserved in evolution, to provide a selective outer membrane export channel for covalently attached polypeptides.
Klauser
T
T
Max-Planck-Institut für Biologie Abteilung Infektionsbiologie, Tübingen, Germany.
Krämer
J
J
Otzelberger
K
K
Pohlner
J
J
Meyer
T F
TF
D000595:Amino Acid Sequence; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism; D004926:Escherichia coli; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000201:enzymology* / Q000235:genetics; D009345:Neisseria meningitidis / Q000201:enzymology* / Q000235:genetics; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases
D017931:DNA Primers; D004269:DNA, Bacterial; D011498:Protein Precursors; D011994:Recombinant Proteins; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase