[Development of an enzyme-linked immunosorbent assay for the detection of antibodies against bovine herpesvirus type 5 (BHV-5) in sheep sera].
The indirect enzyme-linked immunosorbent assay (ELISA) was first developed for the detection of antibodies against BHV-5 in sheep sera. Investigations of various antigen (Ag) preparations revealed that BHV material which had been purified across a sucrose gradient using sonification followed by ultracentrifugation gave satisfactory results during application in ELISA. Optimal coating of the solid phase was achieved with Ag adsorption at 4 degrees C overnight with 5 micrograms of virus material/ml in a carbonate-bicarbonate-buffer, pH 9.7. The background reactions were significantly reduced by blocking the free bonding points with 1% gelafusal after Ag adsorption. As the extinction values of all the sera investigated which a dilution of 1:50 or more below 0.2, the difference threshold between positive and negative sera was set at 0.2. A total of 490 sera from various stocks of sheep were investigated with regard to the occurrence of antibodies against BHV-5 in ELISA. The growing rate of infection with herpesviruses among ruminants and the subsequent economic loss have resulted in the increased significance in the last few years of bovine herpes-virus (BHV) disease.
Lindner
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A
Institut für Mikrobiologie und Tierseuchenlehre, Veterinärmedizinischen Fakultät, Universität Leipzig.
Liebermann
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H
Ambrosius
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005858:Germany / Q000453:epidemiology; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology*
D000914:Antibodies, Viral
Entwicklung eines Enzyme-linked Immunosorbent Assay zum Nachweis von Antikörpern gegen das Bovine Herpesvirus vom Typ 5 (BHV-5) in Schafseren.