Moesin: a cell membrane protein linked with susceptibility to measles virus infection.
Measles virus is a highly contagious virus causing acute and persistent diseases in man, the receptor of which is still not well characterized. We have isolated a monoclonal antibody (mAb), designated mAb 119, which specifically inhibits measles virus infection of susceptible cell lines in a dose-dependent manner. This antibody precipitates a protein with an apparent molecular mass of 75 kDa from 125I surface-labeled cells and its epitope is present on human peripheral blood mononuclear cells, human cell lines, and the African green monkey cell line Vero. Affinity chromatography of detergent-solubilized cell membrane proteins over a Sepharose column with covalently bound mAb 119 led to the partial purification of the 75-kDa protein. Preincubation of measles virus with this affinity-purified protein inhibited measles virus infection dose dependently. Amino acid microsequencing of this protein revealed its identity with the human membrane-organizing extension spike protein moesin, a protein intra- and extracellularly associated with the plasma membrane of cells. Subsequently, an antibody raised against purified moesin (mAb 38/87) was also found to specifically inhibit measles virus infection of susceptible cells and confirmed our data obtained with mAb 119. Our data suggest that moesin is acting as a receptor for measles virus.
Dunster
L M
LM
Institut für Virologie und Immunbiologie, Würzburg, Germany.
Schneider-Schaulies
J
J
Löffler
S
S
Lankes
W
W
Schwartz-Albiez
R
R
Lottspeich
F
F
ter Meulen
V
V
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000494:pharmacology; D000914:Antibodies, Viral / Q000302:isolation & purification / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D002459:Cell Fusion / Q000187:drug effects; D015536:Down-Regulation; D006801:Humans; D007113:Immunity, Innate; D008459:Measles virus / Q000737:chemistry*; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015703:Antigens, CD; D000998:Antiviral Agents; C497857:CD46 protein, human; C497859:Mcp protein, mouse; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D011506:Proteins; C071058:moesin