Detection of c-Ha-ras oncogene expression in pleural and peritoneal smear effusions by in situ hybridization.
This study was undertaken to determine the presence of the c-Ha-ras protooncogene in pleural and peritoneal effusions. Cells from 40 pleural and peritoneal fluids from patients with malignant and nonmalignant diseases were studied by in situ DNA hybridization utilizing a biotinylated probe. The results were correlated with those obtained by conventional cytological methods. A cytological diagnosis of metastatic carcinoma was established in 67% of the patients with malignancy and effusion, and in 6% of the patients with benign disease accompanied by effusion. On the other hand, a relatively high proportion of ras expression was observed in 38% of the cases representing benign and reactive effusions and as high as 88% in the cases with malignant tumor and effusion. These results suggest that the ras oncogene may have an important role in differentially diagnosing benign and reactive mesothelial cells from malignant cells.
Athanassiadou
P P
PP
Cytology and Cytogenetic Department, Laiko Hospital, Athens, Greece.
Veneti
S Z
SZ
Kyrkou
K A
KA
Athanassiades
P H
PH
D001202:Ascitic Fluid / Q000378:metabolism*; D015870:Gene Expression; D011905:Genes, ras; D006801:Humans; D017403:In Situ Hybridization; D010996:Pleural Effusion / Q000378:metabolism*