title: Free radical scavenger depletion in post-ischemic reperfusion brain damage.
abstract: In the present study the influence of pretreatment with various GSH depletors such as buthionine sulfoximine (BSO) and diethylmaleate (DEM) was investigated in rats following cerebral post-ischemic reperfusion. Moreover, the effect of diethyldithiocarbamic acid (DDC), inhibitor of endogenous Cu,Zn-SOD, was evaluated. A significant depletion (40% of control value) of GSH levels was observed 24 h after DEM administration; after 48 h the value reached control levels. BSO showed maximal GSH depletion (59%) 24 h after administration and it was constant for almost 48 h. DDC administration caused a marked decrease (60%) of Cu,Zn-SOD activity 4 h after the injection and induced a marked decrease in percentage of survival with respect to control (untreated, ischemic) rats, when administered 4 h before ischemia. BSO and DEM prolonged the survival time of animals when administered 24 h before ischemia. This last paradoxical effect is unclear at present, but it might be due to an influence on glutamate cascade.
authors.affiliation: Institute of Biochemistry, Faculty of Pharmacy, University of Catania, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Vanella
authors.forename: C
authors.initials: C
authors.lastname: Di Giacomo
authors.forename: V
authors.initials: V
authors.lastname: Sorrenti
authors.forename: A
authors.initials: A
authors.lastname: Russo
authors.forename: C
authors.initials: C
authors.lastname: Castorina
authors.forename: A
authors.initials: A
authors.lastname: Campisi
authors.forename: M
authors.initials: M
authors.lastname: Renis
authors.forename: J R
authors.initials: JR
authors.lastname: Perez-Polo
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D019328:Buthionine Sulfoximine; D004050:Ditiocarb / Q000494:pharmacology; D016166:Free Radical Scavengers; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008298:Maleates / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D008298:Maleates; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D004050:Ditiocarb; D013482:Superoxide Dismutase; C014476:diethyl maleate; D005978:Glutathione