A novel plant glutathione peroxidase-like protein provides tolerance to oxygen radicals generated by paraquat in Escherichia coli.
Citrus salt-stress associated protein (Cit-SAP) reveals significant sequence homology to mammalian glutathione peroxidase (GP). In an attempt to assign biological function to this protein, transformed E. coli cells expressing Cit-SAP were examined for their ability to tolerate free radicals formed by paraquat, an O2- radical forming agent. In the presence of paraquat, the survival rate of the transformed bacteria expressing Cit-SAP was much higher as compared to the wild-type bacteria. The results support the assumption that Cit-SAP is a plant GP-like protein which participate in the enzymatic system aimed at scavenging oxygen free-radicals in plants.
Holland
D
D
Department of Fruit Tree Breeding and Genetics, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Faltin
Z
Z
Perl
A
A
Ben-Hayyim
G
G
Eshdat
Y
Y
D004361:Drug Tolerance; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005609:Free Radicals; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000235:genetics / Q000502:physiology*; D010269:Paraquat / Q000494:pharmacology*; D010957:Plasmids; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014169:Transformation, Bacterial
D005609:Free Radicals; D013481:Superoxides; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D010269:Paraquat