title: Clinical Mycoplasma gallisepticum infection in multiplier breeder and meat turkeys caused by F strain: identification by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, restriction endonuclease analysis, and the polymerase chain reaction.
abstract: In February 1991, a flock of North Carolina multiplier breeder turkeys experienced respiratory signs, sinusitis, airsacculitis, and increased mortality. Mycoplasma gallisepticum (MG) was isolated, and appropriate control measures were initiated. Ultimately, this outbreak involved several breeder flocks of an integrated turkey production company before the last infected flock was identified in May 1991. During this time, MG was also isolated from a flock of commercial layer-type chickens raised as pullets in close proximity to the index turkey flock. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and restriction endonuclease analysis indicated that these isolates were identical to each other and to examples of the vaccinal F strain. Additionally, MG isolates from the affected turkey breeder and layer flocks were identified as MG F strain by use of an F strain-specific DNA probe and polymerase chain reaction. A separate outbreak of MG disease in several meat-turkey flocks of a Midwest producer/processor yielded isolates identified as F strain by the polymerase chain reaction. These studies demonstrated: 1) the utility of newer technologies for disease outbreak investigations; and 2) the potential of MG F strain to cause disease in breeder and meat turkeys under field conditions.
authors.affiliation: Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
authors.forename: D H
authors.initials: DH
authors.lastname: Ley
authors.forename: A P
authors.initials: AP
authors.lastname: Avakian
authors.forename: J E
authors.initials: JE
authors.lastname: Berkhoff
mesh_terms: D000818:Animals; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008297:Male; D009174:Mycoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D009657:North Carolina / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology*; D014422:Turkeys
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes