1 alpha, 25-Dihydroxyvitamin D3 and 19-nor-1 alpha, 25-dihydroxyvitamin D2 suppress immunoglobulin production and thymic lymphocyte proliferation in vivo.
BALB/CANNHSD female mice were intraperitoneally (i.p.) injected with 1,25-dihydroxyvitamin D-3 (1,25-(OH)2D3) (20 ng/day) or 19-nor-1,25-(OH)2D2 (100 ng/day) for 7 days and then given the same dose every other day thereafter until day 17 for measuring cell-mediated immunity and on day 33 for observing immunoglobulin production. To observe Ig production, the mice were immunized with an injection of hemocyanin (150 microgram/0.1 ml, i.p.) on day 8. On the day 7, 15 and 20 after immunization, the IgG1, and on day 17 and 20, the IgG3 levels in 1,25-(OH)2D3 and 19-nor-1,25-(OH)2D2 groups were significantly lower than in the control group. To measure cell-mediated immunity, mice were sensitized with dinitrofluorobenzene (DNFB) on the shaved abdominal skin. The number of thymic lymphocytes and their stimulation index were significantly reduced by 1,25-(OH)2D3 and 19-nor-1,25-(OH)2D2. These results demonstrate that 1,25-(OH)2D3 and 19-nor-1,25-(OH)2D2 can suppress immunoglobulin production and thymic lymphocyte proliferation in vivo. 19-Nor-1,25-(OH)2D2 at doses of 100 ng/day was less effective than 1,25-(OH)2D3 at doses of 20 ng/day.
Yang
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S
Department of Biochemistry, University of Wisconsin-Madison 53706.
Smith
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C
DeLuca
H F
HF
D015652:25-Hydroxyvitamin D 2 / Q000097:blood; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002117:Calcitriol / Q000031:analogs & derivatives / Q000494:pharmacology*; D002118:Calcium / Q000097:blood; D002469:Cell Separation; D053493:Cholestanetriol 26-Monooxygenase; D004139:Dinitrofluorobenzene; D004872:Ergocalciferols / Q000494:pharmacology*; D005260:Female; D006433:Hemocyanins; D007136:Immunoglobulins / Q000096:biosynthesis*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D013250:Steroid Hydroxylases / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects
D004872:Ergocalciferols; D007136:Immunoglobulins; D007166:Immunosuppressive Agents; D015652:25-Hydroxyvitamin D 2; C084656:paricalcitol; D006433:Hemocyanins; D004139:Dinitrofluorobenzene; D013250:Steroid Hydroxylases; D053493:Cholestanetriol 26-Monooxygenase; C506832:Cyp27a1 protein, mouse; D002117:Calcitriol; D002118:Calcium