title: Influence of growth factors on in vitro invasiveness and type IV collagenolysis of human renal cell carcinoma cells.
abstract: We investigated the influence of epidermal growth factor (EGF) and transforming growth factor (TGF)-beta 1 on the in vitro invasion and type IV collagenolytic activity of two new cell lines of renal cell carcinoma (SRCC-1P and SRCC-1M). When cells were treated with EGF or with TGF-beta 1, EGF increased the number of cells penetrating through the reconstituted basement membrane in SRCC-1P. In contrast, TGF-beta 1 suppressed the number of cells penetrating through the membrane in SRCC-1M. In accordance with the invasiveness, EGF enhanced the activity of type IV collagenolysis in SRCC-1P, and TGF-beta 1 suppressed it in SRCC-1M. The growth factors did not affect DNA synthesis of the cells as evaluated by 3H-thymidine incorporation. These results suggest that EGF and TGF-beta 1 can influence the in vitro invasive process of renal cell carcinoma cells through their actions on proteolysis such as type IV collagenolysis.
authors.affiliation: Department of Urology, School of Medicine, Sapporo Medical University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Otani
authors.forename: T
authors.initials: T
authors.lastname: Tsukamoto
authors.forename: N
authors.initials: N
authors.lastname: Masumori
authors.forename: I
authors.initials: I
authors.lastname: Saiki
authors.forename: J
authors.initials: J
authors.lastname: Yoneda
authors.forename: Y
authors.initials: Y
authors.lastname: Kumamoto
mesh_terms: D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology*; D003094:Collagen / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000502:physiology*; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D016212:Transforming Growth Factor beta / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D003094:Collagen