title: Nucleotide sequence and properties of the hrmA locus associated with the Pseudomonas syringae pv. syringae 61 hrp gene cluster.
abstract: The hrmA locus, isolated from Pseudomonas syringae pv. syringae 61, is essential for phenotypic expression of the P. s. pv. syringae 61 hrp cluster in Escherichia coli strains and enables bacteria carrying the hrp/hrm gene cluster to elicit the hypersensitive response (HR) associated with plant disease resistance. The phenotype of P. s. pv syringae 61 hrmA mutants (pathogenicity+, delayed HR) was distinct from that of hrp mutants. The locus was localized to a 3.6-kb BamH1-EcoR1 fragment whose nucleotide sequence was determined. A single open reading frame was identified that encodes for a 41,457-Da protein of unknown biochemical function. Production of the deduced protein product was confirmed by using T7 RNA polymerase-directed expression of the locus and N-terminal sequence analysis of the isolated HrmA. The deduced protein product did not exhibit homology with any of the characterized avr genes or the hrpN product of Erwinia amylovora. Transcription was shown to initiate 37 nucleotides upstream of the translational start from an apparent sigma 70 promoter. Two hrp genes were shown to act as positive transcriptional factors for hrmA expression. Expression of hrmA in P. syringae pv. glycinea race 4 did not exhibit the phenotypic properties of an avr gene or HrpN, but suggested that this locus may serve a regulatory function. A homolog to hrmA was present in strains of only three of the 23 P. syringae pathovars tested.
authors.affiliation: Department of Botany, University of Maryland, College Park 20742.
authors.forename: S
authors.initials: S
authors.lastname: Heu
authors.forename: S W
authors.initials: SW
authors.lastname: Hutcheson
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D014176:Protein Biosynthesis; D011549:Pseudomonas / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014764:Viral Proteins; C085100:hrmA protein, Pseudomonas syringae; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases