title: Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field samples.
abstract: Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.
authors.affiliation: Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, England, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Kyes
authors.forename: A G
authors.initials: AG
authors.lastname: Craig
authors.forename: K
authors.initials: K
authors.lastname: Marsh
authors.forename: C I
authors.initials: CI
authors.lastname: Newbold
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D016054:DNA, Protozoan; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; C047712:S-antigen, Plasmodium falciparum