title: Endothelial seeding of vascular prostheses: a technique of in situ enzymatic retrieval of endothelial cells without vein sacrifice.
abstract: The success of endothelial seeding of vascular prosthetic grafts is largely dependent on endothelial cell retrieval. An in situ cell harvesting technique using enzymatic dissolution in a temporarily excluded segment of the internal jugular vein is described. With this technique the cellular yield is adequate and the internal jugular vein remains patent. This technique allows for high-density seeding in a single stage. In two-stage seeding the excellent yield of cells reduces the duration of in vitro endothelial cell culture and allows for secondary massive and early seeding.
authors.affiliation: Service de Chirurgie Vasculaire, CHU Jean Minjoz, Besançon, France.
authors.forename: J
authors.initials: J
authors.lastname: Combe
authors.forename: J M
authors.initials: JM
authors.lastname: Buniet
authors.forename: P
authors.initials: P
authors.lastname: Hervé
authors.forename: G
authors.initials: G
authors.lastname: Camelot
mesh_terms: D001807:Blood Vessel Prosthesis; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D017364:Collagenases; D004730:Endothelium, Vascular / Q000166:cytology* / Q000637:transplantation; D006801:Humans; D007601:Jugular Veins / Q000166:cytology*; D014654:Vascular Patency / Q000502:physiology
chemical_list: D017364:Collagenases