Hepatic gene therapy: efficient gene delivery and expression in primary hepatocytes utilizing a conjugated adenovirus-DNA complex.
Receptor-mediated endocytosis is an effective method for gene delivery into target cells. We have previously shown that DNA molecules complexed with asialoglycoprotein can be efficiently endocytosed by primary hepatocytes and the internalized DNA can be released from endosomes by the use of a replication-defective adenovirus. Because the DNA and virus enter target cells independently, activity enhancement requires high concentrations of adenoviral particles. In this study, adenoviral particles were chemically conjugated to poly(L-lysine) and bound ionically to DNA molecules. Quantitative delivery to primary hepatocytes was achieved with significantly reduced viral titer when the asialoorosomucoid-poly(L-lysine) conjugate was included in the complex. The conjugated adenovirus was used to deliver a DNA vector containing canine factor IX to mouse hepatocytes, resulting in the expression of significant concentrations of canine factor IX in the culture medium. The results suggest that receptor-mediated endocytosis coupled with an efficient endosomal lysis vector should permit the application of targeted and efficient gene delivery into the liver for gene therapy of hepatic deficiencies.
Cristiano
R J
RJ
Department of Cell Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030.
Smith
L C
LC
Kay
M A
MA
Brinkley
B R
BR
Woo
S L
SL
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001212:Asialoglycoproteins / Q000378:metabolism; D001692:Biological Transport; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics*; D004247:DNA / Q000378:metabolism*; D004285:Dogs; D004705:Endocytosis; D004742:Enhancer Elements, Genetic; D005164:Factor IX / Q000096:biosynthesis* / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009961:Orosomucoid / Q000031:analogs & derivatives / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D001212:Asialoglycoproteins; D009961:Orosomucoid; C017030:asialoorosomucoid; D005164:Factor IX; D004247:DNA; D001616:beta-Galactosidase