title: Detection of Mycobacterium leprae nasal carriers in populations for which leprosy is endemic.
abstract: In order to better understand the role of Mycobacterium leprae nasal carriage in the maintenance of infection reservoirs and transmission of leprosy, we applied a polymerase chain reaction (PCR) that detected a 531-bp fragment of the pra gene of M. leprae on nasal swab specimens collected through a total population survey from individuals living in an area in which leprosy is endemic. Among the total tested population of 1,228 people, 7.8% were found to be PCR positive. PCR positivity was shown to be randomly distributed among the population for which leprosy is endemic. No association was observed between PCR positivity, age, or sex. The observed distribution of PCR positivity among households of different sizes confirmed the expected values, with the exception of two households, each with three people with PCR-positive nasal swab specimens. Although nasal carriage does not necessarily imply infection or excretion of bacilli, the finding of nasal carriage supports the theory of a disseminated occurrence of M. leprae in populations for which leprosy is endemic.
authors.affiliation: Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
authors.forename: P R
authors.initials: PR
authors.lastname: Klatser
authors.forename: S
authors.initials: S
authors.lastname: van Beers
authors.forename: B
authors.initials: B
authors.lastname: Madjid
authors.forename: R
authors.initials: R
authors.lastname: Day
authors.forename: M Y
authors.initials: MY
authors.lastname: de Wit
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002353:Carrier State / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D007918:Leprosy / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D009166:Mycobacterium leprae / Q000235:genetics / Q000302:isolation & purification; D009297:Nasal Mucosa / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial