Novel structural organisation of a Mus musculus DBA/2 chromosome shows a fixed position for the centromere.
Chromosome 1 of the inbred mouse strain DBA/2 shows an unusual polymorphism associated with its centromeric satellite DNA sequences. The minor satellite array has undergone amplification and is present as two blocks separated by major satellite sequences. Both minor satellite blocks appear to carry the sequence motif necessary for CENP-B protein binding. Despite this apparent similarity the functional centromere, as defined by the location of CREST antigens, appears to form only within the more terminal block. The two blocks also vary in that sister chromatid association only occurs with this more terminal block.
Mitchell
A R
AR
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK.
Nicol
L
L
Malloy
P
P
Kipling
D
D
D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D002503:Centromere / Q000648:ultrastructure*; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D002875:Chromosomes / Q000648:ultrastructure*; D004276:DNA, Satellite / Q000032:analysis; D004268:DNA-Binding Proteins; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008811:Mice, Inbred DBA / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012854:Sister Chromatid Exchange
D001324:Autoantigens; C497927:Cenpb protein, mouse; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D004268:DNA-Binding Proteins