[A simple and rapid method capable of detecting methicillin-resistant Staphylococcus aureus (MRSA) by single culture on an agar plate].
A novel simple agar plate capable of detecting methicillin-resistant Staphylococcus aureus (MRSA) by single culture was developed. The agar plate included 1) mannitol and a pH indicator, and 4-methylumbelliferyl phosphoric acid, both for identification of Staphylococcus aureus by its ability to digest mannitol and produce phosphatase; detection of the former by change in color of pH indicator and the latter by fluorescence of 4-methylumbelliferone, 2) oxacillin at a minimum concentration just to eliminate methicillin-sensitive Staphylococcus aureus (MSSA), and 3) phenylethyl alcohol to inhibit growth of most gram negative rods. The method was simple, sensitive, time-saving, and quite effective in the identification of MRSA directly from clinical specimens. Neither false positive nor false negative results were observed by the present method.
Yoshida
S
S
Department of Laboratory Medicine, Hokkaido University School of Medicine, Sapporo.
Shibuya
H
H
Sato
K
K
Shimizu
Y
Y
Chiba
H
H
Matsuno
K
K
Kobayashi
K
K
Saitou
A
A
D000362:Agar; D001431:Bacteriological Techniques; D003470:Culture Media; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000031:analogs & derivatives; D008353:Mannitol; D016106:Methicillin Resistance; D010068:Oxacillin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*
D003470:Culture Media; C005359:4-methylumbelliferyl phosphate; D008353:Mannitol; D006923:Hymecromone; D000362:Agar; D010068:Oxacillin