title: Absorption of radiolabelled soluble insulin in type 1 (insulin-dependent) diabetes: influence of subcutaneous blood flow and anthropometry.
abstract: The rate of absorption of soluble insulin, and its relationships with subcutaneous blood flow (SCBF) and patients' anthropometry, were investigated in 12 male Type 1 (insulin-dependent) diabetic patients of less than 10 yr disease duration (age range: 23-29 yr; body mass index (BMI): 22.2-30.8 kg m-2). Simultaneous assessment of the absorption of 125I-labelled soluble insulin and SCBF (99mTechnetium clearance) were performed, on separate study days, for the anterior abdominal wall, anterior mid-thigh, and the upper arm injection sites. Absorption of 125I soluble insulin, including during the lag-phase, was determined by the two- and three-parameters biexponential models description of residual radioactivity levels at the injection site. Anthropometric measurements utilized were: BMJ, ultrasonic measurement of the subcutaneous adipose tissue layer, and caliper skinfold thickness at anterior abdominal wall, biceps, triceps, anterior mid-thigh and subscapular sites. Rate-constants for absorption of soluble insulin were positively correlated with SCBF (Spearman rank coefficient (rs) = 0.33-0.55; p < 0.01-0.001). An inverse relationship was observed between the duration of the lag-phase and SCBF (rs = -0.28 - -0.54; p < 0.05-0.001). Patients' degree of adiposity was inversely correlated with the rate of soluble insulin absorption (rs = -0.24 - -0.28; p < 0.05) and SCBF (rs = -0.28 - -0.43; p < 0.05-0.001). Consequently, faster absorption of soluble insulin, with shorter lag-phase duration, was noted for the abdominal and thigh sites for non-obese (BMI > 25 kg m-2) than obese patients (BMI < 27.5 kg m-2) (p < 0.05-0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, University of Wales College of Medicine, Health Park, Cardiff, UK.
authors.forename: J P
authors.initials: JP
authors.lastname: Vora
authors.forename: A
authors.initials: A
authors.lastname: Burch
authors.forename: J R
authors.initials: JR
authors.lastname: Peters
authors.forename: D R
authors.initials: DR
authors.lastname: Owens
mesh_terms: D000327:Adsorption; D000328:Adult; D000886:Anthropometry; D015992:Body Mass Index; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism* / Q000503:physiopathology*; D006801:Humans; D007279:Injections, Subcutaneous; D007328:Insulin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D061389:Insulin, Regular, Pork; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply*; D013667:Technetium
chemical_list: D007328:Insulin; D061389:Insulin, Regular, Pork; D007457:Iodine Radioisotopes; D013667:Technetium; C000006:insulin, neutral