IC101, extracellular matrix antagonist produced by Streptomyces sp. MJ202-72F3. Production, isolation, structure determination and biological activity.
In our search for inhibitors of cell adhesion to components of extracellular matrix (ECM), fibronectin, laminin and collagen type IV, we succeeded in finding a novel cyclic hexadepsipeptide antibiotic, named IC101, which was isolated from cultured mycelium of Streptomyces albulus MJ202-72F3. It was purified by centrifugal partition chromatography, preparative reverse phase HPLC and Sephadex LH-20 and was obtained as a white powder. IC101 strongly inhibited cell adhesion to ECM components, suppressed immune responses in vitro and in vivo, and exhibited antimicrobial activity on Gram-positive bacteria.
Ueno
M
M
Institute for Chemotherapy, Microbial Chemistry Research Foundation, Shizuoka, Japan.
Amemiya
M
M
Someno
T
T
Masuda
T
T
Iinuma
H
H
Naganawa
H
H
Hamada
M
M
Ishizuka
M
M
Takeuchi
T
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000187:drug effects*; D005260:Female; D007941:Leukemia P388 / Q000188:drug therapy; D051379:Mice; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D010455:Peptides; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D013302:Streptomyces / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D016326:Extracellular Matrix Proteins; D010455:Peptides; D010456:Peptides, Cyclic; C085032:IC 101