Deuterium NMR spectroscopy of biofluids for the identification of drug metabolites: application to N,N-dimethylformamide.
The metabolism of N,N-dimethylformamide in the Sprague-Dawley rat has been reinvestigated using NMR spectroscopy of urine. In particular, through the use of N,N-dimethylformamide-d7 (DMF-d7) and 2H-NMR spectroscopy, the principal metabolites of this compound have been confirmed in a direct manner. The use of inverse-detected two-dimensional 2H-13C correlation with 13C decoupling aided metabolite identification through the provision of 13C chemical shifts.
Farrant
R D
RD
Department of Physical Sciences, Wellcome Research Laboratories, Beckenham, Kent, UK.
Salman
S R
SR
Lindon
J C
JC
Cupid
B C
BC
Nicholson
J K
JK
D000818:Animals; D003903:Deuterium; D004126:Dimethylformamide / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014556:Urine / Q000737:chemistry*
D004126:Dimethylformamide; D003903:Deuterium