title: Analysis of the ovine respiratory syncytial virus (RSV) G glycoprotein gene defines a subgroup of ungulate RSV.
abstract: Respiratory syncytial virus (RSV) has been isolated from sheep suffering from respiratory tract disease. Since the greatest differences between bovine RSV and human RSV are found on the attachment G protein, we have determined the nucleotide and deduced amino acid sequences of the G gene of ovine RSV. The latter contained 838 nucleotides and had a major open reading frame encoding a protein of 263 residues, and shared 73% nucleotide sequence identity with that of bovine RSV. The deduced amino acid sequence of the ovine RSV G protein showed only 60% amino acid identity with the G protein of bovine RSV. Despite the low level of identity, there were similarities in the predicted hydropathy profiles of the G proteins of ovine and bovine RSV. The intergenic sequences for the SH-G and G-F gene junctions of ovine RSV showed 64 and 57% identity respectively with the corresponding regions of the bovine RSV. Our results indicate that ovine and bovine RSV might be classified as two subgroups of an ungulate RSV.
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park 20742.
authors.forename: S K
authors.initials: SK
authors.lastname: Mallipeddi
authors.forename: S K
authors.initials: SK
authors.lastname: Samal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005814:Genes, Viral / Q000235:genetics*; D015707:HN Protein; D008969:Molecular Sequence Data; D012136:Respiratory Syncytial Viruses / Q000145:classification* / Q000235:genetics*; D012418:Ruminants / Q000382:microbiology*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D015707:HN Protein; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C116204:attachment protein G