Comparison of fluorogenic and conventional membrane filter media for enumerating coliform bacteria.
4-Methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide were added to MacConkey broth and their diagnostic powers for total coliforms (TC) and Escherichia coli, respectively, were tested by membrane filtration at primary isolation. Examining water samples from different sources proved the usefulness of fluorogenic rather than reference media both as regards recovery efficiency and rapidity (possible within 12 h) of analyses. The recoveries obtained by fluorogenic and conventional tests for both TC and E. coli were correlated. Values were comparable in surface water samples, while a higher sensitivity of fluorogenic media was observed in samples of shallow contaminated ground water. Results seem to indicate that the use of fluorogenic membrane filtration analysis for colimetric indicators could be favourably considered especially for sanitary surveying of drinking water.
Cenci
G
G
Department of Hygiene, University of Perugia, Italy.
De Bartolomeo
A
A
Caldini
G
G
D015169:Colony Count, Microbial / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D004755:Enterobacteriaceae / Q000254:growth & development / Q000302:isolation & purification*; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification; D005374:Filtration; D005697:Galactosides; D006923:Hymecromone / Q000031:analogs & derivatives*; D014871:Water Microbiology; D014881:Water Supply
D003470:Culture Media; D005697:Galactosides; D006923:Hymecromone; C026845:4-methylumbelliferyl-galactopyranoside; C034888:4-methylumbelliferyl glucuronide