title: Phosphorylation of synthetic acidic peptides by casein kinase II: evidence for competition with phosphorylation of proteins involved in transcription.
abstract: Phosphorylation of several synthetic acidic peptides by biochemically isolated casein kinase II (CKII) and by cellular and nuclear extracts containing CKII-like activity has been investigated. Especially the synthetic peptide pyroGlu-Asp-Asp-Ser-Asp-Glu-Glu-Asn comprising the carboxy-terminal acidic hepta-peptide of the largest subunit of RNA polymerase II was found to serve as an excellent substrate for purified CKII. Moreover, this peptide reduces the rate of 'in vitro' ATP-dependent stimulation of DNA transcription induced by the proteins in the extracts. Since the peptide itself is also significantly phosphorylated in such assays, it is supposed that it serves as a competitive substrate for the phosphorylation of proteins in the extracts whose phosphorylation seems to be a prerequisite for their activity in the transcription process. This points to the involvement of CKII and substrate(s) of CKII in the process of transcription.
authors.affiliation: Institute of Cell Biology, University of Perugia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Angiolillo
authors.forename: M
authors.initials: M
authors.lastname: Bramucci
authors.forename: V
authors.initials: V
authors.lastname: Marsili
authors.forename: F
authors.initials: F
authors.lastname: Panara
authors.forename: A
authors.initials: A
authors.lastname: Miano
authors.forename: D
authors.initials: D
authors.lastname: Amici
authors.forename: G L
authors.initials: GL
authors.lastname: Gianfranceschi
mesh_terms: D000143:Acids / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive / Q000502:physiology; D047390:Casein Kinase II; D002417:Cattle; D002457:Cell Extracts; D002843:Chromatin / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000143:Acids; D002457:Cell Extracts; D002843:Chromatin; D009687:Nuclear Proteins; D010455:Peptides; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases