title: Nucleotide sequence of the gene coding for Clostridium botulinum (Clostridium argentinense) type G neurotoxin: genealogical comparison with other clostridial neurotoxins.
abstract: The neurotoxin gene from Clostridium botulinum type G was cloned as a series of overlapping DNA fragments generated using polymerase chain reaction (PCR) technology and primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 5'-end of the gene was obtained using a primer based on a conserved region of the nontoxic-nonhaemagglutinin gene lying upstream of the toxin gene. Translation of the nucleotide sequence derived from the cloned PCR fragments demonstrated that the gene encodes a protein of 1297 amino acid residues (rmm 149, 147). Comparative alignment of the determined BoNT/G sequence with those of other clostridial neurotoxins revealed highest sequence relatedness (approx. 58% amino acid identity) with BoNT/B of proteolytic and non-proteolytic C. botulinum. Tetanus toxin (TeTx) and other BoNT types revealed lower levels of relatedness with BoNT/G (approximate range 35-42% amino acid identity).
authors.affiliation: Department of Microbiology, Institute of Food Research, Reading Laboratory, Earley Gate, UK.
authors.forename: K
authors.initials: K
authors.lastname: Campbell
authors.forename: M D
authors.initials: MD
authors.lastname: Collins
authors.forename: A K
authors.initials: AK
authors.lastname: East
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009498:Neurotoxins; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014121:Toxoids / Q000235:genetics*
chemical_list: C035415:Clostridium botulinum toxoid; D017931:DNA Primers; D004269:DNA, Bacterial; D009498:Neurotoxins; D014121:Toxoids; D001905:Botulinum Toxins