[Reproducible model of a bacterial conjunctivitis].
To test the efficiency of antimicrobial substances on bacterial conjunctivitis under standardized conditions a reproducible model is needed. As there are no data in the literature concerning this topic we have developed a model of bacterial conjunctivitis in the rabbit eye. Instillation of a suspension of Haemophilus influenzae, Staphylococcus epidermidis or Staphylococcus aureus into the cul-de-sac did not result in any effect. Thus, standardized techniques of conjunctival incision were used additionally. A follow-up was done for a period of 2 weeks (degree of conjunctival hyperemia, microbiology). Radial incision of the conjunctiva with injection of an S. aureus suspension (50 microliters of ATCC 29213; 1.35 x 10(8) cells) was done mimicking a branch injury. This resulted in a purulent conjunctivitis over a period of 1 week and disappeared within 14 days. The swabs were negative on day 7. This is the first model establishing a reproducible purulent conjunctivitis in the rabbit. Using this model antimicrobial substances may be tested under standardized conditions.
Behrens-Baumann
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Abteilung Augenheilkunde, Universität Göttingen, BRD.
Begall
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T
D000818:Animals; D003228:Conjunctiva / Q000382:microbiology / Q000473:pathology / Q000601:surgery; D003234:Conjunctivitis, Bacterial / Q000150:complications / Q000473:pathology; D004195:Disease Models, Animal; D006192:Haemophilus Infections; D006193:Haemophilus influenzae; D006940:Hyperemia / Q000209:etiology; D007267:Injections; D011817:Rabbits; D015203:Reproducibility of Results; D013203:Staphylococcal Infections; D013211:Staphylococcus aureus; D013212:Staphylococcus epidermidis; D013492:Suppuration
Reproduzierbares Modell einer bakteriellen Konjunktivitis.