Splicing of an anti-sense Alu sequence generates a coding sequence variant for the alpha-3 subunit of a neuronal acetylcholine receptor.
In this report we demonstrate that an alpha-3 acetylcholine receptor subunit transcriptional variant originates through alternative splicing of a complementary sequence of the right arm of an Alu element. This element is located within the 5.1 Kb intron found between exons 5 and 6 of the alpha-3 acetylcholine receptor subunit gene. The transcriptional variant originates from the normal splicing process and carries an in-frame stop codon. If translated, it should encode for a peptide lacking the 4th transmembrane domain of the normal subunit.
Mihovilovic
M
M
Department of Medicine, Duke University DUMC, Durham, NC 27710.
Mai
Y
Y
Herbstreith
M
M
Rubboli
F
F
Tarroni
P
P
Clementi
F
F
Roses
A D
AD
D017398:Alternative Splicing; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D014644:Genetic Variation; D015894:Genome, Human; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000235:genetics*; D011950:Receptors, Cholinergic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D016372:RNA, Antisense; D011950:Receptors, Cholinergic