Characterization of BcgI, a new kind of restriction-modification system.
The BcgI restriction enzyme from Bacillus coagulans is unusual in that it cleaves on both sides of its recognition site, CGAN6TGC, releasing a fragment that includes the site and several bases on each side. We report the organization and nucleotide sequences of the genes for the BcgI restriction-modification system and the properties of the proteins that they encode. The system comprises two adjacent, similarly oriented genes. The proximal gene, bcgIA, codes for a 637-amino acid protein (molecular mass = 71.6 kDa) that resembles certain m6A-specific DNA-methyltransferases, particularly those that constitute the modification subunits of type I restriction-modification systems. The distal gene, bcgIB, codes for a 341-amino acid protein (molecular mass = 39.2 kDa) that resembles none of the sequences in the sequence data bases. The two genes overlap by several nucleotides. Alone, neither protein restricts or modifies DNA, but, together, they form a complex in the proportion A2B that does both. DNA binding assays showed that the DNA-protein complex can be formed only in the presence of both subunits, suggesting that the association of inactive subunits generates the active BcgI enzyme that can bind DNA and then either cleaves or methylates at target site.
Kong
H
H
New England Biolabs, Inc., Beverly, Massachusetts 01915.
Roemer
S E
SE
Waite-Rees
P A
PA
Benner
J S
JS
Wilson
G G
GG
Nwankwo
D O
DO
D000225:Adenine / Q000031:analogs & derivatives / Q000096:biosynthesis; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D008745:Methylation; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011485:Protein Binding; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D000225:Adenine; D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; D009838:Oligodeoxyribonucleotides; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C085188:BcgI methyltransferase; C073736:endodeoxyribonuclease BcgI; C000730567:6-methyladenine