title: Oligonucleotide circularization by template-directed chemical ligation.
abstract: An efficient method for producing the covalent closure of oligonucleotides on complementary templates by the action of BrCN was developed. A rational design of linear precursor oligonucleotides was studied, and the effect of factors such as oligonucleotide concentration and oligomer-template length ratio was evaluated. The efficiency of circularization was shown to correlate well with the secondary structure of the precursor oligomer (as predicted by a simple computer analysis), hairpin-like structures bearing free termini clearly favouring the circularization reaction. A novel idea, consisting of the incorporation of non-nucleotide insertions in the precursor oligomer (namely, 1,2-dideoxy-D-ribofuranose residues), may render this method universal and highly effective. An original set of assays was developed to confirm the circular structure of the covalently closed oligonucleotides.
authors.affiliation: GENSET, Paris, France.
authors.forename: N G
authors.initials: NG
authors.lastname: Dolinnaya
authors.forename: M
authors.initials: M
authors.lastname: Blumenfeld
authors.forename: I N
authors.initials: IN
authors.lastname: Merenkova
authors.forename: T S
authors.initials: TS
authors.lastname: Oretskaya
authors.forename: N F
authors.initials: NF
authors.lastname: Krynetskaya
authors.forename: M G
authors.initials: MG
authors.lastname: Ivanovskaya
authors.forename: M
authors.initials: M
authors.lastname: Vasseur
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
mesh_terms: D001483:Base Sequence; D003488:Cyanogen Bromide / Q000737:chemistry; D004270:DNA, Circular / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D013698:Templates, Genetic
chemical_list: D004270:DNA, Circular; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D003488:Cyanogen Bromide