Characterisation by 1H NMR spectroscopy of oligosaccharides derived from alkali-extractable wheat-flour arabinoxylan by digestion with endo-(1-->4)-beta-D-xylanase III from Aspergillus awamori.
Alkali-extractable wheat-flour arabinoxylan, treated with endo-(1-->4)-beta-D-xylanase III from Aspergillus awamori CMI 142717, was fractionated by Bio-Gel P-2 size exclusion chromatography at 60 degrees C. Column fractions, corresponding to oligosaccharides with degrees of polymerisation from 5 to 10, were collected, and subfractionated by high performance anion-exchange chromatography on CarboPac PA-1. The structures of the oligosaccharides thus obtained were elucidated by 1H NMR spectroscopy, showing chains of (1-->4)-linked beta-D-xylopyranosyl residues differently substituted at O-3 and/or O-2,3 with alpha-L-arabinofuranosyl groups. The structures were different from those obtained with endo-(1-->4)-beta-D-xylanase I of the same xylanolytic enzyme system.
Kormelink
F J
FJ
Department of Food Science, Agricultural University, Wageningen, Netherlands.
Hoffmann
R A
RA
Gruppen
H
H
Voragen
A G
AG
Kamerling
J P
JP
Vliegenthart
J F
JF
D000468:Alkalies; D001089:Arabinose / Q000032:analysis; D002240:Carbohydrate Sequence; D043364:Endo-1,4-beta Xylanases; D005433:Flour; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D017421:Sequence Analysis; D014908:Triticum / Q000737:chemistry*; D014990:Xylans / Q000737:chemistry* / Q000378:metabolism; D014994:Xylose / Q000032:analysis
D000468:Alkalies; D009844:Oligosaccharides; D010936:Plant Extracts; D014990:Xylans; C085118:arabinoxylan; D014994:Xylose; D001089:Arabinose; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases