fliU and fliV: two flagellar genes essential for biosynthesis of Salmonella and Escherichia coli flagella.
The possible functions of two recently described flagellar genes, fliU and fliV, have been examined. Introduction of gene fliC, encoding the bacterial flagellin protein, into a number of flagellin-deficient Salmonella and Escherichia coli strains failed to complement the mutations in these strains, and the FliC flagellin was accumulated in the bacterial cytoplasm. Complementation with fliU and fliV, which map downstream of fliC, restored motility to some of the mutants which became flagellated. After inactivation of either fliU or fliV, such complementation no longer occurred and the flagellin protein accumulated in the cytoplasm, which suggested that both genes are required for the secretion of flagellin and expression of motility. Expression of these genes from high copy number plasmids resulted in the synthesis of exceptionally long flagella and in detection of the FliV protein on polyacrylamide gels.
Doll
L
L
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Frankel
G
G
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002465:Cell Movement / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005407:Flagella / Q000378:metabolism* / Q000502:physiology; D005408:Flagellin / Q000096:biosynthesis / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D013045:Species Specificity; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; C080790:FliU protein, Salmonella muenchen; C080791:FliV protein, Salmonella muenchen; D005408:Flagellin