Two dominant neutralizing antigenic determinants of canine parvovirus are found on the threefold spike of the virus capsid.
The 25-nm diameter parvovirus capsid is assembled from 60 copies of a sequence common to the overlapping VP1 and VP2 proteins. Here we examine the epitope specificity's of 28 monoclonal antibodies (MAb) prepared against canine parvovirus (CPV), feline panleukopenia virus (FPV), and raccoon-dog parvovirus or blue (Arctic) fox parvovirus. Comparing the reactivity of those MAb with various MAb-selected escape mutants, or with natural variants of CPV or mink enteritis virus (MEV) which differ at known sequences, showed that the binding of 20 of those MAb was strongly affected by variations of two regions on the threefold spike of the CPV capsid. One region was adjacent to the tip of the threefold spike, and the second was around VP2 residue 300, on the shoulder of that structure. MAb recognizing both antigenic sites efficiently neutralized the virus infectivity and inhibited hemagglutination. Mutations leading to natural antigenic variation have also been observed in both those sites in naturally variant strains of CPV or MEV, suggesting that they are important antigenic structures on these parvoviruses. The bindings of several MAb were not affected by the mutations at those antigenic sites, indicating that they recognized other, and perhaps conserved, structures.
Strassheim
M L
ML
James A. Baker Institute, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Gruenberg
A
A
Veijalainen
P
P
Sgro
J Y
JY
Parrish
C R
CR
D000818:Animals; D000956:Antigens, Viral / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D002213:Capsid / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D002460:Cell Line; D004285:Dogs; D014644:Genetic Variation; D016056:Immunodominant Epitopes / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D017993:Parvovirus, Canine / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011489:Protein Denaturation; D017422:Sequence Analysis, DNA
D000956:Antigens, Viral; D036022:Capsid Proteins; D016056:Immunodominant Epitopes