Different resistance of mammalian red blood cells to hemolysis by bile salts.
To evaluate why hemolysis of red blood cells (RBC) by bile acids varies in different mammalian species, we determined the mean corpuscular volume (MCV), lipid content and the concentrations of the conjugates of deoxycholate and of NaCl inducing 50% hemolysis of RBC from healthy humans, pigs, horses, cows, sheep and jaundiced humans. A volume of 0.05 mL of washed RBC at 1% hematocrit, which has the same lipid content but different phospholipid composition and number of erythrocytes (owing to the variable MCV), was incubated in taurodeoxycholate (TDC) solution (0-5 mM) to determine the TDC concentration inducing 50% hemolysis (TDC50). The TDC50 was highest in RBC of sheep and decreased within the series sheep > pig > cow > horse > healthy human > jaundiced human, which have generally increasing MCV. The osmotic resistance followed an inverse order, with jaundiced human > healthy human > horse > cow > pig > sheep. Although we found no correlation between the TDC50 and phospholipid composition of the erythrocytes tested, the extent of bile salt-induced hemolysis seemed to depend on both the MCV and the number of erythrocytes in the incubation medium.
Salvioli
G
G
University of Modena, Ospedale Estense, Italy.
Gaetti
E
E
Panini
R
R
Lugli
R
R
Pradelli
J M
JM
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002779:Cholestasis; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects*; D004911:Erythrocyte Volume; D006002:Glycodeoxycholic Acid / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D006736:Horses / Q000502:physiology; D006801:Humans; D009996:Osmotic Fragility / Q000502:physiology; D010743:Phospholipids / Q000032:analysis; D012418:Ruminants / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013109:Sphingomyelins / Q000032:analysis; D013657:Taurodeoxycholic Acid / Q000494:pharmacology
D001647:Bile Acids and Salts; D010743:Phospholipids; D013109:Sphingomyelins; D006002:Glycodeoxycholic Acid; D012965:Sodium Chloride; D013657:Taurodeoxycholic Acid