title: An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies.
abstract: An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies specific to Bordetella bronchiseptica in guineapigs and rabbits was developed. In conventional and SPF colonies of guineapigs and rabbits, the ELISA was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. The ELISA showed a sensitivity of 100% and a specificity of 90% in guineapigs. In rabbits the sensitivity and specificity of the ELISA were 97% and 91% respectively. In rabbit sera from infected colonies, ELISA activity showed a statistically significant correlation with titres obtained in the micro-agglutination test. Since serologically unrelated strains of the bacterium exist, the monitoring of animals for B. bronchiseptica infection by ELISA should be performed with various antigens.
authors.affiliation: Department of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Boot
authors.forename: R H
authors.initials: RH
authors.lastname: Bakker
authors.forename: H
authors.initials: H
authors.lastname: Thuis
authors.forename: J L
authors.initials: JL
authors.lastname: Veenema
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D001885:Bordetella Infections / Q000382:microbiology / Q000662:veterinary; D016950:Bordetella bronchiseptica / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D006168:Guinea Pigs; D011817:Rabbits; D051381:Rats; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial