title: Segregation analysis of the mouse Rb(6.16) translocation in zygotes produced by heterozygous female carriers.
abstract: The segregation products in zygotes of females heterozygous for the mouse Rb(6.16) translocation were studied. Of 191 first-cleavage metaphase zygotes recovered from 16 females mated to chromosomally normal males, 132 were cytogenetically analyzed after sequential G- and C-banding. There were no products of adjacent segregation, since the six chromosomal imbalances were unrelated to the translocation. Alternate segregation was seen in 126 (95.5%) of the zygotes, with 85 being chromosomally normal and 41 carrying the translocation. These results indicate preferential alternate segregation and a significant deviation from the Mendelian 1:1 ratio (P < 0.005) for reciprocal alternate segregants, as seen earlier for males with this translocation. However, the approximately 2:1 ratio for normal-to-balanced segregants in females is lower than the ratios consistently seen for males. This supports the notion that there are different underlying causes of the distortion in the sexes.
authors.affiliation: School of Life and Health Sciences, University of Delaware, Newark 19716.
authors.forename: I P
authors.initials: IP
authors.lastname: Aranha
authors.forename: P A
authors.initials: PA
authors.lastname: Martin-DeLeon
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D005260:Female; D005306:Fertilization; D006580:Genetic Carrier Screening; D008297:Male; D051379:Mice / Q000235:genetics*; D008813:Mice, Inbred ICR; D010063:Ovum / Q000166:cytology; D014178:Translocation, Genetic; D015053:Zygote / Q000166:cytology / Q000502:physiology*