title: Haemagglutinating activities of oral strains of Streptococcus milleri group.
abstract: Of a total 148 strains of Streptococcus milleri group, 66 agglutinated sheep erythrocytes. The haemagglutinating strains were confined to serotypes g, h, i, j or were untypeable, and had no Lancefield group antigens (A to G and K), all of which have been shown to belong to Streptococcus intermedius. Cell surface hydrophobicity did not significantly differ even between the agglutinating and non-agglutinating S. intermedius strains. The haemagglutinating activity of strain 0813-1 (serotype i) was partially sensitive to heat (100 degrees C, 30 min) or trypsin (1 mg/ml, 30 min) treatment, but completely lost after the heat and subsequent trypsin treatments. Only L-arginine, L-lysine (100 mM), mucin, and fetuin (1 mg/ml) partially inhibited the haemagglutination with native bacterial cells but completely inhibited that with heated cells, whereas none was inhibitory in the reaction with trypsin-treated cells. The results suggest that at least two haemagglutinins are involved in the agglutination of the S. intermedius strain and that the heat-stable but trypsin-sensitive haemagglutinin recognizes the receptors on the erythrocyte surface which contain L-arginine and L-lysine at the reactive site.
authors.affiliation: Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Taketoshi
authors.forename: H
authors.initials: H
authors.lastname: Eifuku-Koreeda
authors.forename: T
authors.initials: T
authors.lastname: Yakushiji
authors.forename: M
authors.initials: M
authors.lastname: Inoue
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D004912:Erythrocytes / Q000502:physiology*; D006023:Glycoproteins / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D013045:Species Specificity; D013291:Streptococcus / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D006023:Glycoproteins; D014357:Trypsin