Adenovirus E1A negatively and positively modulates transcription of AP-1 dependent genes by dimer-specific regulation of the DNA binding and transactivation activities of Jun.
Adenovirus E1A proteins inhibit expression of the collagenase gene but activate expression of the c-jun gene. Both effects are mediated by TPA-responsive elements (TREs), the binding sites for members of the AP-1 transcription factor family. By a process that is independent of the retinoblastoma gene product, E1A distinguishes between different AP-1 factors: in vivo binding of Jun/Jun homodimers and Jun/Fos heterodimers to the collagenase TRE is totally blocked by E1A while, in contrast, there is no inhibition of Jun/ATF-2 binding to the TRE sequences in the c-jun promoter. Altered phosphorylation of the DNA binding domain of cJun is not involved in the inhibition of cJun/cJun and cJun/cFos binding. E1A does, however, cause hyperphosphorylation of the transactivation domain of cJun, which is likely to be responsible for the enhanced c-jun transcription by E1A mediated through cJun/ATF-2 heterodimers.
Hagmeyer
B M
BM
Kernforschungszentrum Karlsruhe, Institut für Genetik, Germany.
König
H
H
Herr
I
I
Offringa
R
R
Zantema
A
A
van der Eb
A
A
Herrlich
P
P
Angel
P
P
D051696:Activating Transcription Factors; D017500:Adenovirus E1A Proteins / Q000096:biosynthesis / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D001798:Blood Proteins / Q000378:metabolism; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D017364:Collagenases / Q000096:biosynthesis / Q000235:genetics; D017931:DNA Primers; D004622:Embryo, Mammalian; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D016758:Genes, jun; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012160:Retina; D013539:Simian virus 40 / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection
D051696:Activating Transcription Factors; D017500:Adenovirus E1A Proteins; D001798:Blood Proteins; D017931:DNA Primers; D046911:Macromolecular Substances; D010446:Peptide Fragments; D016755:Proto-Oncogene Proteins c-jun; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D017364:Collagenases; D013755:Tetradecanoylphorbol Acetate