title: Rapid determination of tetracycline antibiotics in serum by reversed-phase high-performance liquid chromatography with fluorescence detection.
abstract: A rapid and accurate determination of tetracycline antibiotics in human serum by reversed-phase high-performance liquid chromatography with fluorescence detection has been developed, based on protein precipitation in serum. Various reagents for precipitation were investigated, and 24% trichloroacetic acid in methanolic solution gave the maximum recovery (at least 94.3%) and interference-free chromatograms of different three tetracyclines. At a concentration of 0.5 micrograms/ml, the precision (relative standard deviation) ranged from 1.12 to 1.94%. In the range 0.04-10.0 micrograms/ml for oxytetracycline and chlorotetracycline and 0.01-10.0 micrograms/ml for tetracycline, linear responses were observed. The detection limits of this method were 10-35 ng/ml for all three antibiotics. The proposed method was applied to the determination of serum concentrations in subjects receiving tetracycline antibiotics.
authors.affiliation: School of Pharmacy, Hokuriku University, Ishikawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Iwaki
authors.forename: N
authors.initials: N
authors.lastname: Okumura
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence; D013754:Tetracyclines / Q000097:blood*
chemical_list: D007202:Indicators and Reagents; D013754:Tetracyclines