Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanase II (pI 9) gene xln2.
The Trichoderma reesei xln2 gene coding for the pI9.0 endoxylanase was isolated from the wild-type strain QM6a. The gene contains one intron of 108 nucleotides and codes for a protein of 223 amino acids in which two putative N-glycosylation target sites were found. Three different T. reesei strains were transformed by targeting a construct composed of the xln2 gene, including its promoter, to the endogenous cbh1 locus. Highest overall production levels of xylanase were obtained using T. reesei ALKO2721, a genetically engineered strain, as a host. Integration into the cbh1 locus was not required for enhanced expression under control of the xln2 promoter.
Saarelainen
R
R
Research Laboratories, Alko Ltd., Helsinki, Finland.
Paloheimo
M
M
Fagerström
R
R
Suominen
P L
PL
Nevalainen
K M
KM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D043364:Endo-1,4-beta Xylanases; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics*; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014170:Transformation, Genetic; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D004271:DNA, Fungal; D012333:RNA, Messenger; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases