title: Nikkomycin-resistant mutants of Mucor rouxii: physiological and biochemical properties.
abstract: We isolated three nikkomycin-resistant mutants of the dimorphic fungus M. rouxii which were physiologically characterized regarding their response to yeast-phase inducing conditions and their sensitivity to bacilysin. Mutant strains G21 and G23, showed a qualitatively normal, though delayed, dimorphic transition and partial cross-resistance to bacilysin. Mutant strain G27 showed an altered dimorphism, producing a high proportion (50%) of hyphal cells, and a wild-type sensitivity to bacilysin. Cell-free extracts from this mutant exhibited an activity of both basal and protease-activated chitin synthetase which was overexpressed as compared with the parental strain and mutants G21 and G23. Results are discussed in terms of the different genetic background of the mutants.
authors.affiliation: Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, México.
authors.forename: N
authors.initials: N
authors.lastname: Ramirez-Ramirez
authors.forename: F
authors.initials: F
authors.lastname: Gutierrez-Corona
authors.forename: E
authors.initials: E
authors.lastname: Lopez-Romero
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002686:Chitin / Q000096:biosynthesis; D002687:Chitin Synthase / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D004352:Drug Resistance, Microbial; D015966:Gene Expression Regulation, Fungal; D007700:Kinetics; D009089:Mucor / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D016296:Mutagenesis; D010641:Phenotype; D013172:Spores, Fungal
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002686:Chitin; C011952:nikkomycin; D002687:Chitin Synthase