[Structure of ADP-ribosylating enzyme and DNA repair].
ADP-ribosylation is a post-translational modification of protein using a respiratory coenzyme, NAD+, as a substrate. Poly(ADP-ribosyl)ation reaction in the nuclei has been thought to be involved in DNA repair. Cloning of poly(ADP-ribose)polymerase cDNA and gene has opened the way for analyzing the structure and functions of poly(ADP-ribose)polymerase at the molecular level. Poly(ADP-ribose)polymerase is a zinc-finger protein and binds to nicks of DNA. This enzyme is activated by binding to DNA and poly(ADP-ribosyl)ates nuclear proteins and poly(ADP-ribose)polymerase itself. Structural analysis of poly(ADP-ribose)polymerase from different classes has shown conserved motifs in the domain structures. In this review, recent advances in this field, including mono-ADP-ribosylation and cyclic ADP-ribose, are described.
Uchida
K
K
Institute of Basic Medical Sciences, University of Tsukuba.
D036002:ADP Ribose Transferases; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004260:DNA Repair; D006801:Humans; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding
D009243:NAD; D004247:DNA; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases