Carcinogens stimulate phosphorylation of ethanolamine derived from increased hydrolysis of phosphatidylethanolamine in C3H/101/2 fibroblasts.
Many human tumors contain high concentrations of ethanolamine phosphate (EtnP). An important question is whether increased formation of EtnP is merely the consequence of cell transformation, or is it associated with the process of carcinogenesis. Here we show that in C3H/10T1/2 embryonic fibroblasts, an established cellular model for the study of carcinogenesis, the environmental carcinogens, 7,12-dimethylbenz[a]anthracene (DMBA) and benzo[a]pyrene (B[a]P) (0.1-1 microgram/ml concentration; 24 h treatment), stimulate phosphorylation of ethanolamine derived from increased hydrolysis of phosphatidylethanolamine. The results suggest that increased formation of EtnP is associated with the early stages of carcinogenesis. This observation may have prognostic value.
Kiss
Z
Z
Hormel Institute, University of Minnesota, Austin, MN 55912.
Crilly
K S
KS
Anderson
W H
WH
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D004983:Ethanolamines / Q000378:metabolism*; D006868:Hydrolysis; D051379:Mice; D008809:Mice, Inbred C3H; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D010767:Phosphorylcholine / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism
D002273:Carcinogens; D004983:Ethanolamines; D010714:Phosphatidylethanolamines; D010767:Phosphorylcholine; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; C005448:phosphorylethanolamine; D017853:Phosphotransferases (Alcohol Group Acceptor); C020125:ethanolamine kinase