The gamma chain of the high-affinity receptor for IgE is a major functional subunit of the T-cell antigen receptor complex in gamma delta T lymphocytes.
T-cell activation is a consequence of the clonotypic T-cell antigen receptor (TCR) binding to an antigen followed by signal transduction via the invariant subunits of the TCR/CD3 complex. gamma delta TCR cells are a small subset of T cells that populate both the epithelial and lymphoid tissues and have unique antigen specificity and function. However, the composition of invariant chains within the gamma delta TCR/CD3 complex has not been well characterized. Here we report that, unlike the majority of alpha beta T cell, gamma delta T cells isolated from spleen and intestinal epithelial tissue express high levels of the gamma chain of the high-affinity receptor for IgE (Fc epsilon RI gamma) as one invariant subunit of their TCR/CD3 complex. Fc epsilon RI gamma exists as both a homodimer and a heterodimer associated with the TCR zeta chain. Moreover, stimulation of the gamma delta TCR results in rapid tyrosine phosphorylation of Fc epsilon RI gamma. Our results suggest that utilization of distinct receptor signaling components may enable the coupling of antigen stimulation to the activation of different signal transduction pathways in alpha beta and gamma delta T cells.
Qian
D
D
Department of Pathology, University of Chicago, IL 60637.
Sperling
A I
AI
Lancki
D W
DW
Tatsumi
Y
Y
Barrett
T A
TA
Bluestone
J A
JA
Fitch
F W
FW
D000818:Animals; D017252:CD3 Complex / Q000096:biosynthesis / Q000737:chemistry; D002478:Cells, Cultured; D004848:Epithelium / Q000276:immunology; D015870:Gene Expression; D015151:Immunoblotting; D007422:Intestines / Q000276:immunology; D008213:Lymphocyte Activation; D046911:Macromolecular Substances; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D017455:Receptors, IgE / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D013154:Spleen / Q000201:enzymology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D017252:CD3 Complex; D046911:Macromolecular Substances; D016692:Receptors, Antigen, T-Cell, gamma-delta; D017455:Receptors, IgE