title: Coated macroporous carriers with oxirane groups and their reactivity.
abstract: Macroporous carriers were prepared by coating controlled pore silicas with copolymers of hydroxyalkyl methacrylate and alkaline dimethacrylate activated by epichlorhydrin (140-1000 mumol/g epoxide group content). The relationship between the preparation conditions (the monomer ratio, the nature of the solvent, the method of activation by epichlorhydrin and the reaction temperature) was studied, as well as the surface characteristics responsible for the carrier's ability to bond (the value of the surface area before and after coating with copolymers and the degree of cross-linking required for the accessibility of the reactive groups). The coupling of various amino compounds, ammonia, aminopropanol, 1,2-diaminoethane, 1,6-diaminohexane and, as a model enzyme, alpha chymotrypsin on to derivatized carriers was investigated as a function of pH, reaction time and the concentration of the compound bonded.
authors.affiliation: Scientific Research Center for Speciality Polymers, Sofia, Bulgaria.
authors.forename: E I
authors.initials: EI
authors.lastname: Zacharieva
authors.forename: C I
authors.initials: CI
authors.lastname: Konstantinov
mesh_terms: D000588:Amines / Q000737:chemistry; D000641:Ammonia / Q000737:chemistry; D001412:Bacillus subtilis / Q000201:enzymology; D002918:Chymotrypsin / Q000737:chemistry; D004146:Dioxanes / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D010450:Endopeptidases / Q000737:chemistry; D004852:Epoxy Compounds / Q000737:chemistry; D005027:Ethylene Oxide / Q000737:chemistry*; D005898:Glass; D007700:Kinetics; D008689:Methacrylates; D012822:Silicon Dioxide; D013499:Surface Properties
chemical_list: D000588:Amines; D004146:Dioxanes; D004337:Drug Carriers; D004852:Epoxy Compounds; D008689:Methacrylates; D012822:Silicon Dioxide; D000641:Ammonia; D010450:Endopeptidases; D002918:Chymotrypsin; C025223:1,4-dioxane; D005027:Ethylene Oxide