Food restriction during organogenesis in rabbits: effects on reproduction and the offspring.
To assess the effects of markedly restricted food intake versus ad libitum feeding or a slightly restricted feeding regimen during the period of organogenesis we fed groups of 16-18 pregnant rabbits Purina Certified High Fiber Chow ad libitum, 150 g/day, 75 g/day, or 15 g/day on Gestation Days 6 to 18 inclusive. Prior to and after organogenesis the animals were provided food ad libitum (ad lib). Clinical observations, body weights, and food and water consumption were recorded daily. On Gestation Day 30 each doe was euthanatized and necropsied, and maternal and fetal data were collected. Each fetus was examined for external, visceral, and skeletal variations and malformations. Ossification parameters were also evaluated. Statistical analyses were conducted in two ways, first comparing the restricted groups to the ad lib group and second comparing the 15 and 75 g/day groups to the 150 g/day group. During Days 6-18, the 15 and 75 g/day groups had significantly decreased weight gain (actual weight loss), compared to the groups fed 150 g/day or ad lib. Water consumption was also significantly decreased in the 15 g/day group during this period, compared to the ad lib group. When food was provided ad lib on Days 19-30 to the restricted groups, weight gain was significantly higher in the 15 and 75 g/day groups than the group previously given 150 g/day and the ad lib group. There were no differences in water consumption during that period. Abortion occurred in three 15 g/day animals.(ABSTRACT TRUNCATED AT 250 WORDS)
Petrere
J A
JA
Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, Michigan 48105.
Rohn
W R
WR
Grantham
L E
LE
Anderson
J A
JA
D000818:Animals; D000013:Congenital Abnormalities / Q000196:embryology; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011817:Rabbits; D012098:Reproduction / Q000502:physiology*