Whole intact tissue electrophoresis of nerve proteins.
We describe a rapid and simple method for analyzing proteins along the length of a nerve tissue using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A long length of nerve tissue is placed on a slab gel, layered with SDS-buffer, and electrophoresed. In this whole-intact-tissue procedure, the in situ differences in location and/or concentration of protein along the length of a nerve tissue are not disturbed by homogenization and dilution prior to electrophoresis.
Sheller
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RA
Department of Zoology, University of Texas at Austin 78712.
Bittner
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GD
D000818:Animals; D003400:Astacoidea; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005069:Evaluation Studies as Topic; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012394:Rosaniline Dyes
D009419:Nerve Tissue Proteins; D012394:Rosaniline Dyes; C048139:Coomassie blue