title: [Human thyroid gland DNA methylase in nodular and diffuse goiter].
abstract: The heterogeneity and some properties of DNA-methylases isolated from nonmalignant human thyroid formations--nodular and diffuse goiters--have been studied. Isoelectrofocusing of methylase preparations produced 6-7 distinct activity peaks distinguished by pI, activity towards Ca2+ and Mg2+, sensitivity towards dithiothreitol and capacity to methylase cytosine into mono-, di- and tripyrimidine blocks in vitro. The degree of DNA methylation in vivo depended on the origin of of the goiter. Analysis of several methylase fractions by two-dimensional electrophoresis performed according to O'Farrell revealed the presence of major polypeptides having similar molecular masses (approximately 36 kDa). Besides the major component, all the preparations under study contained 6-10 characteristic minor components. The data obtained are suggestive of a structural and functional heterogeneity of human methylases.
authors.forename: I I
authors.initials: II
authors.lastname: Nikol'skaia
authors.forename: E V
authors.initials: EV
authors.lastname: Sharkova
authors.forename: E N
authors.initials: EN
authors.lastname: Zenina
authors.forename: N G
authors.initials: NG
authors.lastname: Lopatina
authors.forename: B A
authors.initials: BA
authors.lastname: Atakhanova
authors.forename: A T
authors.initials: AT
authors.lastname: Adylova
authors.forename: A E
authors.initials: AE
authors.lastname: Koneeva
authors.forename: L I
authors.initials: LI
authors.lastname: Kovalev
authors.forename: S S
authors.initials: SS
authors.lastname: Shishkin
mesh_terms: D015254:DNA Modification Methylases / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006044:Goiter, Nodular / Q000201:enzymology*; D006111:Graves Disease / Q000201:enzymology*; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D013961:Thyroid Gland / Q000201:enzymology*
chemical_list: D015254:DNA Modification Methylases
vernacular_title: DNK-metilazy shchitovidnoĭ zhelezy cheloveka pri uzlovom i diffuznom zobe.