A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X-Y homologous gene amelogenin.
A rapid, simple and reliable sex test that entails PCR amplification of a segment of the X-Y homologous gene amelogenin has been developed. We used a single pair of primers spanning part of the first intron which generated 106-bp and 112-bp PCR products from the X and Y homologues, respectively, that can be analyzed simply by agarose gel electrophoresis. Less than 1 ng of template DNA is required for gender assignment, and the test has been automated by the fluorescent tagging of the PCR products that are then quantitated during electrophoresis by automated fluorescence-detection technology. Quantitation enables sex chromosome aneuploidy to be determined, and the amelogenin intron sequence can also be co-amplified with several highly polymorphic microsatellite loci, thereby providing a combined gender/identity DNA test.
Sullivan
K M
KM
Central Research and Support Establishment, Forensic Science Service, Berks, UK.
Mannucci
A
A
Kimpton
C P
CP
Gill
P
P
D053523:Amelogenin; D000782:Aneuploidy; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004276:DNA, Satellite; D003746:Dental Enamel Proteins / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis
D053523:Amelogenin; D004276:DNA, Satellite; D003746:Dental Enamel Proteins; D005456:Fluorescent Dyes; D004247:DNA