Inhibition of bovine mononuclear cell proliferation, interleukin-2 synthesis, protein-tyrosine kinase and leukotriene B4 production by a protein-tyrosine kinase inhibitor, genistein.
Genistein, an isoflavanoid compound, a selective inhibitor of protein-tyrosine kinase (PTK), inhibited PHA-stimulated bovine peripheral blood mononuclear cell proliferation (PBMC), interleukin-2 (IL-2) production, phosphorylation of PTK p56lck. Further, genistein also inhibited leukotriene B4 production from A-23187 stimulated cultures. Our data suggest that the PTK plays an important role in the signal transduction of bovine PBMC proliferation.
Atluru
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Department of Medicine/Nephrology, Hennepin County Medical Center, Minneapolis, MN 55404.
Gudapaty
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D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019833:Genistein; D007376:Interleukin-2 / Q000037:antagonists & inhibitors*; D007529:Isoflavones / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D010835:Phytohemagglutinins; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D011863:Radioimmunoassay / Q000662:veterinary; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology
D007376:Interleukin-2; D007529:Isoflavones; D010835:Phytohemagglutinins; D007975:Leukotriene B4; D000001:Calcimycin; D019833:Genistein; D011505:Protein-Tyrosine Kinases