Theoretical considerations for the application of competitive polymerase chain reaction to the quantitation of a low abundance mRNA: estrogen receptor.
The necessary theoretical considerations for the development of a correct quantitative analysis of a low abundance messenger RNA (mRNA), estrogen receptor mRNA, by competitive polymerase chain reaction (PCR) are presented together with a series of experimental data. When compared to other methodologies currently utilized for RNA quantitation, this PCR application proved to be a very reliable, rapid and sensitive method. Furthermore, the PCR-based quantitative method described is of particular interest since it does not require the use of radiolabeled compounds.
Santagati
S
S
Milano Molecular Pharmacology Laboratory, University of Milan, Italy.
Bettini
E
E
Asdente
M
M
Muramatsu
M
M
Maggi
A
A
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D018076:DNA, Complementary / Q000096:biosynthesis; D005260:Female; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011960:Receptors, Estrogen / Q000235:genetics*; D014599:Uterus / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger; D011960:Receptors, Estrogen