Developmental and tissue-specific modulation of rat galactose-1-phosphate uridyltransferase steady state messenger RNA and specific activity levels.
To assess the role of genetic regulation as a modulating factor in the variability of rat tissue galactose-1-phosphate uridyltransferase (GALT) sp act, we have determined steady state GALT mRNA and sp act in rat liver during postnatal development. Steady state GALT mRNA levels increase from birth to d 5 and subsequently decrease toward adult levels. GALT sp act mirrors the mRNA pattern. A survey of steady state mRNA and GALT sp act of several adult rat tissues revealed marked tissue differences with a good correlation of the two parameters. Liver had the highest GALT mRNA and sp act; kidney, ovary, and heart had similar but lower mRNA and sp act; skeletal muscle and testes had the least GALT mRNA and enzyme sp act. These findings suggest that genetic regulation is important in the variable expression of GALT tissue sp act.
Heidenreich
R A
RA
Department of Pediatrics, University of Pennsylvania, Children's Hospital of Philadelphia 19104.
Mallee
J
J
Rogers
S
S
Segal
S
S
D000375:Aging / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology* / Q000254:growth & development; D008168:Lung / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D010053:Ovary / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000201:enzymology; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase / Q000096:biosynthesis / Q000378:metabolism*
D012333:RNA, Messenger; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase