Determination of L-methionine-dl-sulphoxide in tissue extracts.
The amino acid fraction from rat liver, heart and skeletal muscle was prepared by the separation of sulphosalicylic acid extract on Dowex 50 H+ form. The presence of L-methionine-dl-sulphoxide in these extracts was identified and compared by three independent chromatographic methods: ion-exchange, Pico-Tag and reversed-phase high-performance liquid chromatography after precolumn derivatisation with diethylethoxymethylenemalonate. Quantitative data indicate that L-methionine-dl-sulphoxide is present in the intracellular pool at the levels of free methionine.
Scislowski
P W
PW
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK.
Harris
I
I
Pickard
K
K
Brown
D S
DS
Buchan
V
V
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D007202:Indicators and Reagents; D008110:Liver Extracts / Q000032:analysis; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives* / Q000032:analysis; D009132:Muscles / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014020:Tissue Extracts / Q000032:analysis*
D007202:Indicators and Reagents; D008110:Liver Extracts; D014020:Tissue Extracts; D008715:Methionine; C013111:methionine sulfoxide