Regulation of c-MYC protein expression in the developing rat cerebellum by phosphoinositide turnover.
Using developing rat cerebellum, we examined the correlation between the turnover of phosphoinositide (PI) and c-myc expression. The 32P incorporation into polyphosphoinositides changed remarkably with advancing age. It reached a maximum value on PND 7, and then decreased gradually until PND 14. Immunostaining by anti-PIP2 antibody changed in parallel. The expression of c-myc mRNA was also changed developmentally, showing a peak on PND 7; whereas c-MYC protein expression showed a peak on PND 10. Together, these results suggest that c-myc expression is regulated by PI turnover during the early developing stage of rat cerebellum.
Takahashi
M
M
Department of Psychiatry, School of Medicine, Yokohama City University, Japan.
Toyoshima
S
S
Miyazawa
A
A
Horikoshi
T
T
Yoshioka
T
T
D000375:Aging; D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000033:anatomy & histology / Q000378:metabolism*; D005786:Gene Expression Regulation; D016762:Genes, fos; D016758:Genes, jun; D016259:Genes, myc; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010716:Phosphatidylinositols / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D010716:Phosphatidylinositols; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger