title: Internucleosomal DNA cleavage involved in ischemia-induced neuronal death.
abstract: Pyramidal neurons of the hippocampal CA1 are known to be particularly vulnerable to transient ischemia resulting in "delayed neuronal death". Recent studies using aurintricarboxylic acid suggested that ischemia- or excitotoxin-induced neuronal death should share intracellular mechanisms in common with apoptosis. It is, however, unclear about involvement of endonucleases. Here using a transient (5 min) forebrain ischemia model in gerbils, we found that internucleosomal DNA fragmentation developed between 48 and 54 hr recirculations, accompanied with simultaneous or slightly preceding destruction of microtubule-associated protein 2. These results suggest that endonucleases, maybe activated by elevated intracellular Ca2+, play an important role in delayed neuronal death as well as in apoptosis.
authors.affiliation: First Department of Internal Medicine, Osaka University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Ohtsuki
authors.forename: A
authors.initials: A
authors.lastname: Taguchi
authors.forename: K
authors.initials: K
authors.lastname: Mikoshiba
authors.forename: T
authors.initials: T
authors.lastname: Yanagihara
authors.forename: T
authors.initials: T
authors.lastname: Kamada
mesh_terms: D000818:Animals; D016923:Cell Death; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D009707:Nucleosomes / Q000378:metabolism*; D011712:Pyramidal Tracts / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D008869:Microtubule-Associated Proteins; D009707:Nucleosomes; D004247:DNA