Demonstration of membrane-associated phospholipase A2 in cultivated heart muscle cells by immunogold-technique in surface replicas.
Recently we produced mAb's to phospholipase A2 (PIA2) from bee venom for different purposes (allergen-identification and -standardization). Obviously one of these monoclonal antibodies "5D5" binds to a conserved (cross reactive) epitope of membrane PIA2 of intact cells. In this study we demonstrate binding of 5D5 to membrane associated phospholipase A2 of rat heart muscle cells by means of two-step immunogold technique in combination with replica technique.
Kriegsmann
J
J
Institute of Pathology, Friedrich Schiller University, Jena, Germany.
Müller
W D
WD
Richter
W
W
Wunderlich
J
J
Wallukat
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D010741:Phospholipases A / Q000276:immunology / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D010741:Phospholipases A; D054467:Phospholipases A2