title: Analysis of albumin deposits on hydroxylated siloxane films. Implications for surface treatment of medical devices.
abstract: The authors have developed methods to enhance albumin binding to modified silicone rubber (SR) films. An intermediate bifunctional coupling agent, polyvinylmethyl siloxane-comethyl-1-ethanol siloxane (PVMS-CO-MES), is prepared from a cyclic tetramer, vinyl-methyl siloxane, by an oxymercuration-demercuration reaction, and cross-linked to silicone rubber under mild peroxide catalytic conditions. Free mercury on the surface was obtained under many reaction conditions and is shown to materially enhance 125I-labeled albumin binding. The mechanism most likely occurs via disulfide bond breakage, protein denaturation, and aggregation. The possible role of iodine-mercury bonds, an artefactual source, is ruled out with the aid of total internal reflectance-fluorescence measurements of the albumin adsorption rate constant. Although in situ albumin aggregation via disulfide bond breakage is a potentially attractive method for biocompatible protein gel formation, the toxicity of mercury makes the current method unfit for clinical practice.
authors.affiliation: Biomedical Engineering Program, University of Texas Southwestern Medical Center at Dallas 75235-9031.
authors.forename: K D
authors.initials: KD
authors.lastname: Nelson
authors.forename: C C
authors.initials: CC
authors.lastname: Tsai
authors.forename: E A
authors.initials: EA
authors.lastname: Kulik
authors.forename: V I
authors.initials: VI
authors.lastname: Sevastianov
authors.forename: A
authors.initials: A
authors.lastname: Constantinescu
authors.forename: R C
authors.initials: RC
authors.lastname: Eberhart
mesh_terms: D000327:Adsorption; D004864:Equipment and Supplies; D006801:Humans; D012709:Serum Albumin; D012711:Serum Albumin, Radio-Iodinated; D012833:Siloxanes; D013499:Surface Properties
chemical_list: D012709:Serum Albumin; D012711:Serum Albumin, Radio-Iodinated; D012833:Siloxanes