Effects of danazol on endometrial DNA synthesis in rats.
The suppressive effects of Danazol, an isoxazol derivative of the synthetic steroid 17 alpha-ethinyltestosterone, on endometrial DNA synthesis were investigated in rats by immunohistochemistry with bromodeoxyuridine (BrdU) and DNA-synthesizing enzyme assays. Rats treated with Danazol for 14 days at 17-19 weeks of age showed a decrease of plasma gonadotropins associated with ovarian hypofunction, persistent diestrus, and a smaller number of corpora lutea in ovary, resulting in no BrdU-immunoreactive (S-phase) cells in endometrial epithelium and lower activity of thymidine kinase in the uterus compared with control.
Suzuki
S
S
Medical Research Institute, Tokyo Medical and Dental University, Japan.
Sakamoto
S
S
Kudo
H
H
Sassa
S
S
Sugiura
Y
Y
Kuwa
K
K
Kasahara
N
N
Mori
T
T
Nagasawa
H
H
D000311:Adrenal Glands / Q000033:anatomy & histology; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D003613:Danazol / Q000494:pharmacology*; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004971:Estrus / Q000502:physiology; D005260:Female; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000097:blood; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000033:anatomy & histology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013937:Thymidine Kinase / Q000378:metabolism; D013940:Thymidylate Synthase / Q000378:metabolism; D014599:Uterus / Q000033:anatomy & histology
D007986:Luteinizing Hormone; D004247:DNA; D013940:Thymidylate Synthase; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine; D003613:Danazol