Characterization of defective phorbol ester responses in a low secreting rat basophilic leukemia (RBL-2H3) cell variant.
The role of protein kinase C (PKC) in the signaling mechanism that stimulates the release of mediators from rat mast cells, for which the RBL-2H3 cell line is a model, is at present unresolved. Current evidence suggests that PKC activation alone is an insufficient stimulus, although it can modulate mast cell exocytosis induced by other agents. In this article we characterize a variant of the RBL-2H3 cell line that has a reduced capacity for mediator secretion in response to an IgE-mediated antigen-induced stimulation. The outcome of our study suggests that at least two PKC isotypes are active in RBL-2H3 cells, and affect the positive and negative modulation of the secretory response.
Monk
P N
PN
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Bingham
B R
BR
Ahmad
T B
TB
Helm
B A
BA
D000199:Actins / Q000187:drug effects / Q000378:metabolism; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D000941:Antigens / Q000187:drug effects; D002460:Cell Line; D003432:Cross-Linking Reagents; D004705:Endocytosis / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D011493:Protein Kinase C / Q000502:physiology; D051381:Rats; D017455:Receptors, IgE / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000199:Actins; D000941:Antigens; D003432:Cross-Linking Reagents; D017455:Receptors, IgE; D019830:Adenosine-5'-(N-ethylcarboxamide); D000431:Ethanol; D011493:Protein Kinase C; D000241:Adenosine; D013755:Tetradecanoylphorbol Acetate