title: Initiation at closely spaced replication origins in a yeast chromosome.
abstract: Replication of eukaryotic chromosomes involves initiation at origins spaced an average of 50 to 100 kilobase pairs. In yeast, potential origins can be recognized as autonomous replication sequences (ARSs) that allow maintenance of plasmids. However, there are more ARS elements than active chromosomal origins. The possibility was examined that close spacing of ARSs can lead to inactive origins. Two ARSs located 6.5 kilobase pairs apart can indeed interfere with each other. Replication is initiated from one or the other ARS with equal probability, but rarely (< 5%) from both ARSs on the same DNA molecule.
authors.affiliation: Department of Genetics SK-50, University of Washington, Seattle 98195.
authors.forename: B J
authors.initials: BJ
authors.lastname: Brewer
authors.forename: W L
authors.initials: WL
authors.lastname: Fangman
mesh_terms: D015825:Chromosomes, Fungal / Q000378:metabolism*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000096:biosynthesis*; D004587:Electrophoresis, Agar Gel; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D004262:DNA Restriction Enzymes