Crystallization and X-ray diffraction study of recombinant platelet-derived endothelial cell growth factor.
Crystals of recombinant platelet-derived endothelial cell growth factor (PD-ECGF) were obtained by the hanging drop vapour diffusion technique. The crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions a = 63.7 A, b = 70.4 A, c = 219.6, alpha = beta = gamma = 90 degrees, and probably contain a single dimer in the asymmetric unit. Diffraction to a minimum Bragg spacing of 3.5 A has been obtained using a synchrotron X-ray source.
Spraggon
G
G
University of Oxford, Department of Biochemistry, U.K.
Stuart
D
D
Ponting
C
C
Finnis
C
C
Sleep
D
D
Jones
Y
Y
D001792:Blood Platelets / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D016228:Endothelial Growth Factors / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
D016228:Endothelial Growth Factors; D011994:Recombinant Proteins