Esterase-30 (ES-30) of the house mouse: biochemical characterization and genetics of a new carboxylesterase isozyme linked to cluster-2 loci on chromosome 8.
A new carboxylesterase isozyme (EC 3.1.1.1), designated ES-30, is described in mouse liver. Two phenotypes were distinguished, ES-30A, a possible null type, was found in SPE/Pas and in other lines derived from Mus spretus, and ES-30B was found in BALB/cJ and other laboratory inbred strains. ES-30B is characterized by a distinct electrophoretic band when stained using 5-bromoindoxyl acetate as the substrate. After isolation and purification from other esterases by ion-exchange chromatography and molecular sieving, the molecular mass was estimated by two independent methods to be 62 and 64 kDa, respectively. The activity of ES-30B is higher in adult males than in females and can be stimulated in vivo by testosterone. The distribution of phenotypes on the progeny of a backcross series suggests a separate locus, Es-30, with the allele a for absence and b for presence of the isozyme. Locus Es-30 is shown to be closely linked to Es-2 and to Es-7 of cluster-2 on chromosome 8. The gene order Es-9--Got-2--(Es-2, Es-7, Es-30) is suggested.
Ronai
A
A
Abteilung für Chemische Pathologie, Universität Freiburg, Bundesrepublik Deutschland.
Berning
W
W
Gaa
A
A
von Deimling
O
O
D000375:Aging / Q000378:metabolism; D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008040:Genetic Linkage; D007123:Immunoelectrophoresis, Two-Dimensional; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010641:Phenotype; D013045:Species Specificity; D013739:Testosterone / Q000378:metabolism
D007527:Isoenzymes; D013739:Testosterone; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C469537:Es30 protein, mouse