title: Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid.
abstract: A polymerase chain reaction (PCR) procedure coupled to an enzyme-linked oligonucleotide sorbent assay (ELOSA; a PCR-ELOSA) identified all 24 serotypic variants of bluetongue virus (BTV) without identifying any of six viruses belonging to the related epizootic hemorrhagic disease virus serogroup. The PCR-ELOSA detected 0.01 50% cell culture infectious doses of each serotype of BTV. The sensitivity and serogroup-wide specificity of the PCR-ELOSA may enable it to replace the more expensive, time-consuming, and biohazardous methods used in the identification of BTV.
authors.affiliation: Diagnostic Virology Laboratory, U.S. Department of Agriculture, Ames, Iowa 50010.
authors.forename: J B
authors.initials: JB
authors.lastname: Katz
authors.forename: A D
authors.initials: AD
authors.lastname: Alstad
authors.forename: G A
authors.initials: GA
authors.lastname: Gustafson
authors.forename: K M
authors.initials: KM
authors.lastname: Moser
mesh_terms: D001483:Base Sequence; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003124:Colorimetry; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D015345:Oligonucleotide Probes