Construction of a series of congenic mice with recombinant chromosome 1 regions surrounding the genetic loci for resistance to intracellular parasites (Ity, Lsh, and Bcg), DNA repair responses (Rep-1), and the cytoskeletal protein villin (Vil).
The interval of mouse chromosome 1 extending from Idh-1 to Pep-3 harbors the natural resistance gene Ity/Lsh/Bcg; it controls the outcome of infection with Salmonella typhimurium, Leishmania donovani, and several Mycobacterium species. This region also contains a DNA repair gene, Rep-1, which determines the rapidity with which double-strand breaks in chromatin are repaired. BALB/cAnPt and DBA/2N mice differ in their phenotypic expression of these genes. To generate appropriate strains of mice for the study of these genes, a series of 10 C.D2 congenic strains recombinant across a 28-centimorgan interval of mouse chromosome 1 extending from Idh-1 to Pep-3 were derived from crosses of the C.D2-Idh-1 Pep-3 congenic strain back to BALB/cAn. Analyses of these recombinant strains will allow the correlation of biological-immunological phenotypes with defined genetic regions.
Mock
B A
BA
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
Holiday
D L
DL
Cerretti
D P
DP
Darnell
S C
SC
O'Brien
A D
AD
Potter
M
M
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D004260:DNA Repair; D005796:Genes; D008040:Genetic Linkage; D006239:Haplotypes; D007113:Immunity, Innate; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008840:Microfilament Proteins / Q000235:genetics; D012481:Salmonella Infections, Animal / Q000235:genetics
D002352:Carrier Proteins; D008840:Microfilament Proteins; C026615:villin