Inverse relationship between the induction of human sperm capacitation and spontaneous acrosome reaction by various biological fluids and the superoxide scavenging capacity of these fluids.
Capacitation of spermatozoa is essential for fertilization, and can be induced by various agents or biological fluids. Previous reports have shown that foetal cord serum (FCS) and the superoxide anion trigger human sperm hyperactivation and capacitation, and that superoxide dismutase (SOD) prevents these processes. We investigated: (1) the capacity of seminal plasma (SP) and follicular fluid (FF) (whole, or fractionated into high and low molecular weight components), in the presence or absence of SOD, to induce the spontaneous acrosome reaction (no stimulant needed, AR) and capacitation (as measured by the lysophosphatidyl-choline-induced AR, LPC-AR); (2) a possible relationship between the levels of AR and capacitation obtained with these biological fluids and the superoxide scavenging capacity of the same fluids. The highest levels of LPC-AR were obtained with FF ultrafiltrate (48 +/- 6%), followed by SP ultrafiltrate (31.9 +/- 0.8%), FF (30 +/- 5%), dialysed FF (27 +/- 4%), and finally, by FCS ultrafiltrate (23 +/- 1%), SP (21 +/- 1%) and dialysed SP (18.9 +/- 0.8%). A similar order of potency for the fluids existed when sperm AR was studied, the levels of AR observed ranging from 16 +/- 2% to 5.3 +/- 0.8% after incubation with FF ultrafiltrate and SP respectively. None of these treatments had detrimental effects on sperm motility. In the presence of SOD, there was always an important reduction (52-86%) of the AR and LPC-AR observed.(ABSTRACT TRUNCATED AT 250 WORDS)
de Lamirande
E
E
Urology Research Laboratory, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
Eiley
D
D
Gagnon
C
C
D000177:Acrosome / Q000502:physiology*; D001826:Body Fluids / Q000502:physiology*; D005260:Female; D015571:Follicular Fluid / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D008297:Male; D012661:Semen / Q000502:physiology*; D013075:Sperm Capacitation / Q000502:physiology*; D013482:Superoxide Dismutase / Q000502:physiology*
D008244:Lysophosphatidylcholines; D013482:Superoxide Dismutase