title: The hepatitis B virus X gene product transactivates the HIV-LTR in vivo.
abstract: It has previously been shown that the hepatitis B virus (HBV) X gene product, HBx, transactivates homologous and heterologous transcriptional regulatory sequences of viruses, including the human immunodeficiency virus type 1 (HIV1) long terminal repeat (LTR), and various cellular genes in vitro. To evaluate the transactivating function of HBx in vivo, we generated transgenic mice carrying the X open reading frame under the control of the human antithrombin III (ATIII) gene regulatory sequences. These mice express the 16 Kd HBx protein in the liver, as demonstrated by immunoprecipitation studies. Crossbreeding of HBx mice with transgenics carrying either the chloramphenicol acetyl transferase (CAT) bacterial or the lacZ reporter gene driven by the HIV1-LTR allowed us to demonstrate, for the first time, the in vivo transactivating function of HBx protein.
authors.affiliation: Laboratoire de Genetique et Patologie Experimentale, INSERM Institut Cochin de Genetique Moleculaire, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Balsano
authors.forename: O
authors.initials: O
authors.lastname: Billet
authors.forename: M
authors.initials: M
authors.lastname: Bennoun
authors.forename: C
authors.initials: C
authors.lastname: Cavard
authors.forename: A
authors.initials: A
authors.lastname: Zider
authors.forename: G
authors.initials: G
authors.lastname: Grimber
authors.forename: G
authors.initials: G
authors.lastname: Natoli
authors.forename: P
authors.initials: P
authors.lastname: Briand
authors.forename: M
authors.initials: M
authors.lastname: Levrero
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D015497:HIV-1 / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D015533:Transcriptional Activation; D054334:Viral Regulatory and Accessory Proteins; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D000990:Antithrombin III; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase