title: Angiotensin-converting enzyme activity in serum and bronchoalveolar lavage fluid after damage to the alveolo-capillary barrier in the human lung.
abstract: OBJECTIVE
  Angiotensin-converting enzyme (ACE) is considered as a possible marker for endothelial cell damage in serum or bronchoalveolar lavage fluid. This hypothesis was tested during cardiac surgery and during the adult respiratory distress syndrome.
  
  
  METHODS
  We used patients with an expected different degree of endothelial cell damage. ACE levels in serum and bronchoalveolar lavage fluid were compared with indirect markers of alveolo-capillary barrier integrity.
  
  
  METHODS
  Interdisciplinary team in a university hospital.
  
  
  METHODS
  13 Cardiac surgery patients received no glucocorticoids and 13 others received 2 g methylprednisolone before extracorporeal circulation. Thirteen patients were used as controls and 15 patients had nonseptic adult respiratory distress syndrome. All underwent bronchoalveolar lavage for ACE determination.
  
  
  RESULTS
  At different times during surgery serum angiotensin-converting enzyme levels were not significantly different between the two groups. In post-operative bronchoalveolar lavage fluid, angiotensin-converting enzyme levels were significantly higher in patients who received corticoids (27.8 +/- 1.7 U/l, mean +/- SEM), compared to patients without corticoids (19.8 +/- 1.4 U/l), control patients (18.2 +/- 1.3 U/l) or patients with full blown non-septic adult respiratory distress syndrome (18.8 +/- 1.1 U/l). There were no correlations between lavage angiotensin-converting enzyme and other parameters for alveolo-capillary membrane integrity in the lavage fluid such as the number of neutrophil cells, albumin or protein concentration, and between lavage angiotensin-converting enzyme and PaO2/FIO2 ratio during lavage.
  
  
  CONCLUSIONS
  Angiotensin-converting enzyme activity in serum or bronchoalveolar lavage fluid does not reflect damage of endothelial cells or damage of alveolocapillary integrity in acute pulmonary disease.
authors.affiliation: Department of Anesthesiology, Sint Jansziekenhuis, Genk, Belgium.
authors.forename: R F
authors.initials: RF
authors.lastname: De Jongh
authors.forename: W A
authors.initials: WA
authors.lastname: De Backer
authors.forename: R
authors.initials: R
authors.lastname: Mohan
authors.forename: P G
authors.initials: PG
authors.lastname: Jorens
authors.forename: F J
authors.initials: FJ
authors.lastname: van Overveld
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D001992:Bronchoalveolar Lavage Fluid; D001999:Bronchoscopy; D006348:Cardiac Surgical Procedures; D005938:Glucocorticoids / Q000627:therapeutic use; D006801:Humans; D007432:Intraoperative Period; D008775:Methylprednisolone / Q000627:therapeutic use; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D012128:Respiratory Distress Syndrome / Q000201:enzymology*
chemical_list: D015415:Biomarkers; D005938:Glucocorticoids; D007703:Peptidyl-Dipeptidase A; D008775:Methylprednisolone