title: Expression cloning of an insect diuretic hormone receptor. A member of the calcitonin/secretin receptor family.
abstract: Insect diuretic hormones and their receptors regulate fluid and ion secretion and thus are attractive targets for the design of novel insect control agents. A complementary DNA clone encoding a corticotropin-releasing factor-related diuretic hormone receptor from the tobacco hornworm Manduca sexta was isolated by expression cloning in COS-7 cells. The receptor consists of 395 amino acids and contains seven putative transmembrane domains. The expressed receptor binds M. sexta diuretic hormone, as well as several related insect diuretic peptides with high affinity. Furthermore, each of these peptides stimulate adenylate cyclase in COS-7 cells transfected with the receptor. The M. sexta diuretic hormone receptor is homologous to the receptors for calcitonin, secretin, vasoactive intestinal peptide, parathyroid hormone, glucagon-like peptide 1, growth hormone-releasing hormone, pituitary adenylate cyclase-activating polypeptide, and glucagon. The M. sexta diuretic hormone receptor is the first nonmammalian member of this family to be identified.
authors.affiliation: Sandoz Agro, Inc., Research Division, Palo Alto, California 94304.
authors.forename: J D
authors.initials: JD
authors.lastname: Reagan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D007301:Insect Hormones / Q000378:metabolism*; D019476:Insect Proteins; D008969:Molecular Sequence Data; D009036:Moths; D018003:Receptors, Calcitonin / Q000235:genetics*; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D011964:Receptors, Gastrointestinal Hormone / Q000235:genetics* / Q000378:metabolism; D018000:Receptors, Peptide / Q000235:genetics*; D012633:Secretin / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D007301:Insect Hormones; D019476:Insect Proteins; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D011964:Receptors, Gastrointestinal Hormone; D018000:Receptors, Peptide; C029937:secretin receptor; D012633:Secretin