Structural characterization of feruloyl oligosaccharides from spinach-leaf cell walls.
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose, and O-alpha-L-arabinofuranosyl-(1-->3)-O-(2-O-trans-feruloyl-alpha-L-a rabino- furanosyl)-(1-->5)-L-arabinofuranose.
Ishii
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Forestry and Forest Products Research Institute, Ibaraki, Japan.
Tobita
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D001089:Arabinose / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry*; D005656:Fungal Proteins; D005690:Galactose; D006026:Glycoside Hydrolases; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014675:Vegetables / Q000737:chemistry*
D003373:Coumaric Acids; D005656:Fungal Proteins; D009844:Oligosaccharides; C004999:ferulic acid; D001089:Arabinose; D006026:Glycoside Hydrolases; C035212:driselase; D005690:Galactose