title: An in situ hybridization technique for the study of B19 human parvovirus replication in bone marrow cell cultures.
abstract: An in situ hybridization technique using digoxigenin labelling was developed to study B19 infection. By using appropriate DNA probes, transcription of structural and non-structural genes was detected in bone marrow cell cultures. Such a simple system is useful to the study of B19-cell interactions in non-permissive cell lines.
authors.affiliation: Laboratory of Microbiology, Saint-Louis Hospital, Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Vassias
authors.forename: S
authors.initials: S
authors.lastname: Perol
authors.forename: L
authors.initials: L
authors.lastname: Coulombel
authors.forename: M C
authors.initials: MC
authors.lastname: Thebault
authors.forename: P H
authors.initials: PH
authors.lastname: Lagrange
authors.forename: F
authors.initials: F
authors.lastname: Morinet
mesh_terms: D001483:Base Sequence; D001853:Bone Marrow / Q000382:microbiology*; D002478:Cells, Cultured; D015342:DNA Probes; D004076:Digoxigenin; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007963:Leukocytes, Mononuclear; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics
chemical_list: D015342:DNA Probes; D004076:Digoxigenin