Recovery of fibrinogen in cryoprecipitate pasteurized in the presence of sucrose and glycine.
The effect of sucrose (60% w/w) and 1 M glycine as thermal stabilizers for fibrinogen in cryoprecipitate was studied. Sucrose (9.2 g) and glycine (0.9 g) were dissolved in 6 g of cryoprecipitate and the solution was pasteurized at 60 degrees C for 10 h. The preparation was then dialyzed for 20 h in phosphate buffered saline (PBS), lyophilized, stored for one week at -40 degrees C and resuspended in distilled water. The recovery of total proteins and fibrinogen in the final product averaged 66.4 +/- 4.1% and 43.8 +/- 6.4% of the initial contents, respectively (mean +/- SEM, N = 9). The pasteurization of cryoprecipitate in the presence of PBS (control experiments) produced extensive precipitation, which is characteristic of protein denaturation. Thus, this method partially protected fibrinogen and other proteins in cryoprecipitate from inactivation by prolonged exposure to heat during pasteurization.
Reis
F M
FM
Red Cross Blood Bank Groningen-Drenthe, Groningen, The Netherlands.
de-Koning
B
B
Das
P C
PC
Smit-Sibinga
C T
CT
D011232:Chemical Precipitation; D015925:Cryopreservation / Q000379:methods*; D003956:Dialysis; D005340:Fibrinogen / Q000302:isolation & purification*; D005998:Glycine / Q000494:pharmacology*; D006801:Humans; D013395:Sucrose / Q000494:pharmacology*
D013395:Sucrose; D005340:Fibrinogen; D005998:Glycine