title: How frequent are correlated changes in families of protein sequences?
abstract: A loss-of-function point mutation in a protein is often rescued by an additional mutation that compensates for the original physical change. According to one hypothesis, such compensation would be most effective in maintaining a structural motif if the two mutated residues were spatial neighbors. If this hypothesis were correct, one would expect that many such compensatory mutations have occurred during evolution and that present-day protein families show some degree of correlation in the occurrence of amino acid residues at positions whose side chains are in contact. Here, a statistical theory is presented which allows evaluation of correlations in a family of aligned protein sequences by assigning a scalar metric (such as charge or side-chain volume) to each type of amino acid and calculating correlation coefficients of these quantities at different positions. For the family of myoglobins it is found that there is a high correlation between fluctuations in neighboring charges. The correlation is close to what would be expected for total conservation of local charge. For the metric side-chain volume, on the other hand, no correlation could be found.
authors.affiliation: Max-Planck-Institut für Biophysikalische Chemie, Abteilung Membranbiophysik, Göttingen, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Neher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009154:Mutation; D009211:Myoglobin / Q000737:chemistry*; D011487:Protein Conformation; D016415:Sequence Alignment; D017421:Sequence Analysis / Q000379:methods; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
chemical_list: D009211:Myoglobin