title: Specific repression of the yeast silent mating locus HMR by an adjacent telomere.
abstract: The yeast silent mating loci HML and HMR are located at opposite ends of chromosome III adjacent to the telomeres. Mutations in the N terminus of histone H4 have been previously found to derepress the yeast silent mating locus HML to a much greater extent than HMR. Although differences in the a and alpha mating-type regulatory genes and in the cis-acting silencer elements do not appear to strongly influence the level of derepression at HMR, we have found that the differential between the two silent cassettes is largely due to the position of the HMR cassette relative to the telomere on chromosome III. While HML is derepressed to roughly the same extent by mutations in histone H4 regardless of its chromosomal location, HMR is affected to different extends depending upon its chromosomal positioning. We have found that HMR is more severely derepressed by histone H4 mutations when positioned far from the telomere (cdc14 locus on chromosome VI) but is only minimally affected by the same mutations when integrated immediately adjacent to another telomere (ADH4 locus on chromosome VII). These data indicate that the degree of silencing at HMR is regulated in part by its neighboring telomere over a distance of at least 23 kb and that this form of regulation is unique for HMR and not present at HML. These data also indicate that histone H4 plays an important role in regulating the silenced state at both HML and HMR.
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles 90024.
authors.forename: J S
authors.initials: JS
authors.lastname: Thompson
authors.forename: L M
authors.initials: LM
authors.lastname: Johnson
authors.forename: M
authors.initials: M
authors.lastname: Grunstein
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000648:ultrastructure; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005809:Genes, Regulator; D006657:Histones / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000648:ultrastructure; D016615:Telomere / Q000648:ultrastructure; D014170:Transformation, Genetic
chemical_list: D006657:Histones