Electron microscopic observation of cotton cellulose degradation by exo- and endo-type cellulases from Irpex lacteus.
The interaction of two highly purified cellulases, exo- and endo-type cellulases from Irpex lacteus, with pure cotton and amorphous cellulose was investigated by electron microscopy. The morphological observations indicated that exo- and endo-type cellulases are both strongly adsorbed on the internal microfibril of cotton fiber before enzymatic hydrolysis, and then initiate their action toward the internal cellulose microfibrils with retention of the original shape. The two cellulases, however, caused considerably different morphological changes in cotton cellulose, and each cellulase seems to degrade native cellulose with a distinct mode of action. In the hydrolysis of cotton with exo-type cellulase, deep transverse cracks were produced and they extended from the fiber surface to the lumen structure located inside the fibers. In contrast, it was found that there were no deep cracks on fibers treated with endo-type cellulase, but severe internal erosion and cavitation occurred along fibril or microfibril layers inside the fibers. Thus, the degradation of cotton by exo- and endo-type cellulases yielded quite different morphological patterns, while little difference was found for regenerated celluloses. The mode of enzymatic hydrolysis of cellulose shown by cellulases with different degrees of randomness (exo and endo types) appears to be markedly affected by the fine structure of cellulose fibers.
Hoshino
E
E
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, Nagano.
Sasaki
Y
Y
Mori
K
K
Okazaki
M
M
Nisizawa
K
K
Kanda
T
T
D000918:Antibody Specificity; D002480:Cellulase / Q000276:immunology / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D003368:Gossypium; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011124:Polyporaceae / Q000201:enzymology*
D002482:Cellulose; D002480:Cellulase