title: Histone acetylation and gene induction in human cells.
abstract: An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet derived growth factor B chain (PDGF-B), a gene which is induced to strong expression upon differentiation. A high level of acetylation was observed before gene induction and no change seen following induction. This implies that core histone acetylation is an essential precondition for transcription.
authors.affiliation: Biophysics Laboratories, University of Portsmouth, UK.
authors.forename: A L
authors.initials: AL
authors.lastname: Clayton
authors.forename: T R
authors.initials: TR
authors.lastname: Hebbes
authors.forename: A W
authors.initials: AW
authors.lastname: Thorne
authors.forename: C
authors.initials: C
authors.lastname: Crane-Robinson
mesh_terms: D000107:Acetylation; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002843:Chromatin; D006657:Histones; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D004247:DNA; D011505:Protein-Tyrosine Kinases; D013755:Tetradecanoylphorbol Acetate