Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid).
We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 mumol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 mumol/day.
Agbaba
D
D
Institute for Medical Biochemistry, University Clinical Center, Belgrade, Serbia.
Stojanov
M
M
Rajacic
S
S
Zivanov-Stakic
D
D
Majkic-Singh
N
N
D000310:Adrenal Gland Neoplasms / Q000175:diagnosis / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000706:statistics & numerical data; D003720:Densitometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D010673:Pheochromocytoma / Q000175:diagnosis / Q000652:urine; D011786:Quality Control; D012016:Reference Values; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D014642:Vanilmandelic Acid / Q000652:urine*
D014642:Vanilmandelic Acid