Evaluation of a probe hybridisation serotyping method for group A rotavirus.
Serotype-specificity and sensitivity of oligonucleotide probes to serotype human rotaviruses was assessed. Probes could detect as little as 6.3 ng of homologous RNA and none reacted with as much as 100 ng of heterologous RNA. Northern-blot analysis revealed that probes reacted with one of genomic segments 7, 8 or 9 of corresponding serotypes.
Ali
A
A
International Centre for Diarrhoeal Disease Research, Bangladesh ICDDR,B.
Bingnan
F
F
Unicomb
L E
LE
Rahim
Z
Z
Hossain
A
A
Tzipori
S
S
D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D015345:Oligonucleotide Probes; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification*; D012680:Sensitivity and Specificity; D012703:Serotyping
D015345:Oligonucleotide Probes; D012367:RNA, Viral