The human cytochrome b5 gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively.
Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b5 gene and two of its pseudogenes (psgb(5)1 and psgb(5)2) unambiguously to chromosomes 18, 14, and 20. These localizations were confirmed and extended to 18q23, 14q31-32.1, and 20p11.2 by using a combination of nonisotopic in situ hybridization of chromosomal spreads and the polymerase chain reaction analysis of DNA samples isolated from somatic cell hybrids retaining deletions or translocations of chromosome 18.
Giordano
S J
SJ
Department of Biochemistry, NEOUCOM, Rootstown, OH 44272.
Yoo
M
M
Ward
D C
DC
Bhatt
M
M
Overhauser
J
J
Steggles
A W
AW
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D002890:Chromosomes, Human, Pair 20; D006224:Cricetinae; D015786:Cytochromes b5 / Q000235:genetics*; D004247:DNA / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011544:Pseudogenes / Q000235:genetics*; D014178:Translocation, Genetic / Q000235:genetics
D004247:DNA; D015786:Cytochromes b5