title: Crystallization and preliminary X-ray analysis of leukemia inhibitory factor.
abstract: Leukemia inhibitory factor (LIF) is a polyfunctional molecule with significant and diverse biological activities. LIF is a glycoprotein secreted by a number of different cell types in vitro. It is induced in fibroblasts, lymphocytes, monocytes and astrocytes by various inducers such as serum, TNF, interleukin-IP and EGF. Due to extensive and variable glycosylation the molecular weight can range from 38 to 67 kDA. The biological functions of LIF are mediated through a receptor and a signal transducer, gp130, which is also used by factors like interleukin-6 (IL-6), cilliary neurotropic factor (CNTF), and oncostatin M (OSM). Here, we report the crystallization of the non-glycosylated human-like LIF expressed in E. coli. The present crystals diffract to 2.0 A using synchrotron radiation. They belong to the monoclinic space group C2, and the cell dimensions are a = 61.5 A, b = 45.3 A, c = 77.7 A and beta = 112.3 degrees.
authors.affiliation: EMBL, Hamburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Betzel
authors.forename: M
authors.initials: M
authors.lastname: Visanji
authors.forename: Z
authors.initials: Z
authors.lastname: Dauter
authors.forename: R
authors.initials: R
authors.lastname: Fourme
authors.forename: W
authors.initials: W
authors.lastname: Weber
authors.forename: U
authors.initials: U
authors.lastname: Marnitz
authors.forename: T
authors.initials: T
authors.lastname: Boone
authors.forename: J
authors.initials: J
authors.lastname: Pope
authors.forename: J
authors.initials: J
authors.lastname: Miller
authors.forename: N
authors.initials: N
authors.lastname: Hawkins
mesh_terms: D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006131:Growth Inhibitors / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D015850:Interleukin-6; D007525:Isoelectric Focusing; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D011994:Recombinant Proteins