title: Uptake of fluorescein isothiocyanate (FITC)-fractionated heparin by rat parenchymal hepatocytes in primary culture.
abstract: The distribution of fractionated heparin in a primary culture of rat parenchymal hepatocytes was investigated optically using the fluorescence labelled drug and confocal imaging system with an inverted fluorescence microscope. The cell-associated fluorescein isothiocyanate (FITC)-fractionated heparin was observed to increase in conjunction with incubation time and also to localize, suggesting an internalization to cell organella with the exception of the nuclei.
authors.affiliation: Department of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Muranishi
authors.forename: M
authors.initials: M
authors.lastname: Haba
authors.forename: H
authors.initials: H
authors.lastname: Yuasa
mesh_terms: D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D005591:Chemical Fractionation; D016650:Fluorescein-5-isothiocyanate; D006493:Heparin / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006493:Heparin; D016650:Fluorescein-5-isothiocyanate