Conversion of human fibroblasts to tissue macrophages by the Snyder-Theilen sarcoma virus (ST:FeSV): tumouricidal effects on K-562 and Daudi tumour cell lines in monolayers and in agar suspensions.
We have previously demonstrated the conversion of human fibroblasts (HF) to tissue macrophages by transduction with the Snyder-Theilen feline sarcoma virus (ST:FeSV) [1-3]. The ST:FeSV-induced TM have been characterized both phenotypically and functionally, including their tumouricidal potential against colon adenocarcinoma (LS 180) cells. The present results show that ST:FeSV-induced TM produced significant lysis of K-562 tumour cells, but essentially no lysis of the Daudi tumour cells. Lysis of K-562 tumour cells by the ST:FeSV-induced TM was considerably more effective in 1% than in 10% FCS. Addition of TNF-alpha caused only a slight increase in the extent of lysis of K-562 tumour cells by the ST:FeSV-induced TM. Coincubation of the ST:FeSV-induced TM with K-562 cells in agar medium resulted in the inhibition of tumour cell proliferation. The results indicate that ST:FeSV-induced TM are potent oncocytotoxic agents of K-562 tumour cells, but are considerably less effective against the Daudi tumour cells.
Kopelovich
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Laboratory of Cancer Genetics and Cancer Prevention, Department of Veterans Affairs, Medical Research, Bay Pines, FL 33504.
D000595:Amino Acid Sequence; D001790:Blood Physiological Phenomena; D002051:Burkitt Lymphoma / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002630:Chemotactic Factors / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004549:Elastin / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000382:microbiology; D015681:Fusion Proteins, gag-onc / Q000502:physiology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000382:microbiology / Q000502:physiology*; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D005253:Sarcoma Viruses, Feline / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
D002630:Chemotactic Factors; D003470:Culture Media; D015681:Fusion Proteins, gag-onc; D008070:Lipopolysaccharides; D010446:Peptide Fragments; D009240:N-Formylmethionine Leucyl-Phenylalanine; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma; D004549:Elastin; D013755:Tetradecanoylphorbol Acetate