title: Microtiter enzyme-linked immunosorbent assay using recombinant derived antigens versus western blot in the confirmation of presence of antibodies against the human immunodeficiency virus type 1.
abstract: A microtiter enzyme-linked immunosorbent assay using recombinant derived antigens was compared with the Western blot (Dupont) in the confirmation of the presence of antibodies against the human immunodeficiency virus type 1 (HIV-1). Of 104 sera (104 individuals) that were negative by a screening ELISA, 91 were also negative by both confirmation assays. In three sera only the microtiter assay was found to be indeterminate, and in nine other sera only Western blot. The only microtiter assay positive serum was from a male patient at risk for infection with HIV. 279 sera from 83 patients were found positive by screening. Of these, 223 sera were positive in both confirmation assays, and no serum was negative. Only one serum was indeterminate by the microtiter ELISA in contrast to 55 sera, including follow-up samples from 25 patients, most of whom had AIDS, by Western blot (Dupont criteria). However, the number of Western blot indeterminate sera decreased substantially applying less stringent criteria for interpretation. In conclusion, the microtiter ELISA performed well as a confirmation test for the presence of antibodies against HIV-1. In addition, the results demonstrate that in the microtiter assay the envelope peptide kp41 is highly discriminative in detecting anti-HIV-1 negative and anti-HIV-1 positive sera.
authors.affiliation: Department of Clinical Microbiology, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Roosendaal
authors.forename: G J
authors.initials: GJ
authors.lastname: van Kamp
authors.forename: C
authors.initials: C
authors.lastname: Mulder
authors.forename: J
authors.initials: J
authors.lastname: Calliauw
authors.forename: J
authors.initials: J
authors.lastname: Kempers
authors.forename: M
authors.initials: M
authors.lastname: Dirks
authors.forename: E C
authors.initials: EC
authors.lastname: Wolters
authors.forename: R W
authors.initials: RW
authors.lastname: ten Kate
mesh_terms: D015492:AIDS Serodiagnosis / Q000379:methods*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens; D015658:HIV Infections / Q000175:diagnosis*; D018023:HIV Seronegativity; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D011994:Recombinant Proteins
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D011994:Recombinant Proteins