A lectin from the liverwort Marchantia polymorpha L.
Bryophytes have been screened for lectins. From the liverwort Marchantia polymorpha (Marchiantiales) a lectin could be purified to homogeneity using a combination of ultrafiltration, size exclusion chromatography and ion exchange chromatography. SDS polyacrylamide gel electrophoresis, size exclusion chromatography and electrospray mass spectroscopy showed that the lectin is a monomeric protein with a M(r) of 16,134.64 +/- 2.93. Marchantia polymorpha lectin agglutinates erythrocytes of different mammalia and exhibits carbohydrate specificity against complex carbohydrate structures. This is the first report of a lectin isolated from liverworts.
Adam
K P
KP
Fachrichtung 12.3 der Universität des Saarlandes, Pharmakognosie und Analytische Phytochemie, D-66041 Saarbrücken, Germany.
Becker
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D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006384:Hemagglutination; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000302:isolation & purification*; D008970:Molecular Weight; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D014462:Ultrafiltration
D037102:Lectins; D037121:Plant Lectins