Effect of ADP-ribosylation reaction on bull testis chromatin.
Polynucleosomes prepared from bull testis nuclei were characterized: DNA length, by agarose gel electrophoresis, and distribution of poly(ADP-ribose)polymerase activity, by incubation with 0.64mM NAD were determined. Maximal activity was found in nucleosome fractions of 3-5 units. Chromatin fragments (5-2 kbp polynucleosomes) were analysed by circular dichroism in both native and ADP-ribosylated forms. The spectrum of the endogenously ADP-ribosylated polynucleosomes, compared to the native fraction, exhibited a higher ellipticity value and a shift of the cross-over point towards the lowest wavelengths, behaving like an H1-depleted chromatin.
Faraone-Mennella
M R
MR
Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Italy.
De Lucia
F
F
Palomba
L
L
Quesada
P
P
Farina
B
B
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D008297:Male; D009707:Nucleosomes / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011487:Protein Conformation; D013737:Testis / Q000648:ultrastructure*
D002843:Chromatin; D009707:Nucleosomes; D000246:Adenosine Diphosphate Ribose; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases