An in situ hybridization technique for the study of B19 human parvovirus replication in bone marrow cell cultures.
An in situ hybridization technique using digoxigenin labelling was developed to study B19 infection. By using appropriate DNA probes, transcription of structural and non-structural genes was detected in bone marrow cell cultures. Such a simple system is useful to the study of B19-cell interactions in non-permissive cell lines.
Vassias
I
I
Laboratory of Microbiology, Saint-Louis Hospital, Paris, France.
Perol
S
S
Coulombel
L
L
Thebault
M C
MC
Lagrange
P H
PH
Morinet
F
F
D001483:Base Sequence; D001853:Bone Marrow / Q000382:microbiology*; D002478:Cells, Cultured; D015342:DNA Probes; D004076:Digoxigenin; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007963:Leukocytes, Mononuclear; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics
D015342:DNA Probes; D004076:Digoxigenin