title: Characterization of an Anaplasma ovis isolate from desert bighorn sheep in southern California.
abstract: To understand the role of bighorn sheep (Ovis canadensis) in the epidemiology of anaplasmosis, we recovered a field isolate from a suspected enzootic area in southern California (USA). Whole blood was collected from three desert bighorn sheep (Ovis canadensis nelsoni) and inoculated into a susceptible splenectomized domestic sheep, calf and a susceptible spleen-intact bighorn sheep. No infection occurred in the calf, but a detectable infection developed in both the domestic sheep and bighorn sheep 24 days after inoculation. The infection in both domestic and bighorn sheep resulted in severe clinical disease but was resolved with the use of tetracycline. Using monoclonal antibodies and DNA probes, we confirmed that the isolate was Anaplasma ovis.
authors.affiliation: Animal Disease Research Unit, United States Department of Agriculture, Pullman, Washington 99164.
authors.forename: W
authors.initials: W
authors.lastname: Goff
authors.forename: D
authors.initials: D
authors.lastname: Stiller
authors.forename: D
authors.initials: D
authors.lastname: Jessup
authors.forename: P
authors.initials: P
authors.lastname: Msolla
authors.forename: W
authors.initials: W
authors.lastname: Boyce
authors.forename: W
authors.initials: W
authors.lastname: Foreyt
mesh_terms: D000709:Anaplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000097:blood / Q000453:epidemiology / Q000382:microbiology*; D000818:Animals; D000829:Animals, Domestic; D000835:Animals, Wild; D000907:Antibodies, Bacterial / Q000097:blood; D002140:California / Q000453:epidemiology; D002417:Cattle; D015342:DNA Probes; D004269:DNA, Bacterial / Q000097:blood; D004912:Erythrocytes / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006400:Hematocrit / Q000662:veterinary; D009693:Nucleic Acid Hybridization; D015995:Prevalence; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D015342:DNA Probes; D004269:DNA, Bacterial