High-resolution fluorescence mapping of 46 DNA markers to the short arm of human chromosome 1.
We describe a high-resolution cytogenetic map for 46 DNA markers previously assigned to the short arm of human chromosome 1. Using fluorescence in situ hybridization on simultaneously R-banded prometaphase chromosomes, a refined map position was found for 45 probes. For 6 of these probes, additional hybridization sites were observed and for another 7 probes, conflicting results were found with regard to previous localizations. For some probes with overlapping map positions, probe order could be determined by dual-color hybridization on elongated chromosomes. The present high-resolution map can be used to refine the previously published composite map and also provides additional landmarks for the construction of a contig map of the short arm of chromosomes 1.
Van Roy
N
N
Department of Medical Genetics, University Hospital, Gent, Belgium.
Laureys
G
G
Versteeg
R
R
Opdenakker
G
G
Speleman
F
F
D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D004247:DNA; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence
D005819:Genetic Markers; D004247:DNA