title: Herpesvirus ICP18.5 and DNA-binding protein genes are conserved in equine herpesvirus-1.
abstract: The genome of equine herpesvirus-1 (EHV-1) contained three open reading frames (ORFs) in a 3.9 kbp BamHI-SmaI fragment at 0.38-0.41 map units in the long unique region. The most 5' ORF encoded the carboxy terminus of a protein with 45-55 percent amino acid homology to the DNA-binding proteins (ICP8-DBP) of four other alpha-herpesviruses. The middle ORF translated to a polypeptide of 775 residues with 43-55% homology to the ICP18.5 proteins. The most 3' ORF encoded the EHV-1 glycoprotein B (gB) gene. Three mRNAs of 4.3, 4.4-4.8, and 3.5-3.9 kb (corresponding to the three sequenced ORFs) were all transcribed from the same strand. The gene order of this group was conserved in all herpesviruses examined.
authors.affiliation: School of Biological Sciences, Macquarie University, Sydney, N.S.W., Australia.
authors.forename: C W
authors.initials: CW
authors.lastname: Bell
authors.forename: J M
authors.initials: JM
authors.lastname: Whalley
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D005814:Genes, Viral; D016679:Genome, Viral; D006564:Herpesviridae / Q000235:genetics; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C040495:glycoprotein B, Simplexvirus; C069185:DNA cleavage and packaging proteins, Herpesvirus