title: Structure of the glycosyl-phosphatidylinositol membrane anchor of acetylcholinesterase from the electric organ of the electric-fish, Torpedo californica.
abstract: The structure of the glycan moiety of the glycosyl-phosphatidylinositol (GPI) membrane anchor from Torpedo californica (electric fish) electric-organ acetylcholinesterase was solved using n.m.r., methylation analysis and chemical and enzymic micro-sequencing. Two structures were found to be present: Glc alpha 1-2Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6myo-inositol and Glc alpha 1-2Man alpha 1-2Man alpha 1-6(GalNAc beta 1-4)Man alpha 1-4GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.
authors.affiliation: Department of Biochemistry, University of Dundee, Scotland, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Mehlert
authors.forename: L
authors.initials: L
authors.lastname: Varon
authors.forename: I
authors.initials: I
authors.lastname: Silman
authors.forename: S W
authors.initials: SW
authors.lastname: Homans
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000201:enzymology; D004557:Electric Organ / Q000201:enzymology*; D006026:Glycoside Hydrolases; D017261:Glycosylphosphatidylinositols / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D014101:Torpedo
chemical_list: D017261:Glycosylphosphatidylinositols; D009844:Oligosaccharides; D000110:Acetylcholinesterase; D010727:Phosphoric Diester Hydrolases; D006026:Glycoside Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase