title: Anoxia and corporal smooth muscle dysfunction: a model for ischemic priapism.
abstract: The hemodynamics of penile flaccidity, erection and detumescence requires corporal smooth muscle to function across a wide variation in pO2. The present study describes the effect of anoxia on corporal smooth muscle response to field stimulation and pharmacologic agonists and antagonists of erection. The response of isolated strips of rabbit corpus cavernosal tissue to field stimulation, phenylephrine, bethanechol, ATP and KCL was determined under oxygenated and anoxic conditions. The results can be summarized as follows: 1) Anoxia eliminated spontaneous contractile activity and reduced basal tissue tension to a minimum. 2) Neither field stimulation nor pharmacological agents (ATP, bethanechol, isoproterenol) could relax basal tension below that induced by anoxia alone. 3) Under anoxic conditions alpha-adrenergic agonists produced poorly sustained phasic contractile responses; anoxia eliminated tonic contractile responses to phenylephrine. 4) In normoxic conditions field stimulation of smooth muscle precontracted with phenylephrine produced frequency-dependent graded relaxations; under anoxic conditions field stimulation yielded contractile responses at all frequencies. Our data suggest that corporal smooth muscle tone, spontaneous contractile activity, the contractile response to alpha-agonists and field stimulated relaxation depend on the state of corporal oxygenation. The inability of alpha-stimulation to induce a tonic contraction of corporal smooth muscle under anoxia in vitro parallels the failure of penile injection of alpha-adrenergic agonists to relax ischemic priapism.
authors.affiliation: Division of Urology, Hospital of the University of Pennsylvania, Philadelphia 19104.
authors.forename: G A
authors.initials: GA
authors.lastname: Broderick
authors.forename: D
authors.initials: D
authors.lastname: Gordon
authors.forename: J
authors.initials: J
authors.lastname: Hypolite
authors.forename: R M
authors.initials: RM
authors.lastname: Levin
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology; D004558:Electric Stimulation; D007511:Ischemia / Q000150:complications; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000503:physiopathology*; D010100:Oxygen / Q000502:physiology; D010413:Penis / Q000098:blood supply / Q000187:drug effects; D010656:Phenylephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011317:Priapism / Q000209:etiology / Q000503:physiopathology*; D011817:Rabbits
chemical_list: D010656:Phenylephrine; D011189:Potassium Chloride; D010100:Oxygen