An approach to the determination of endotoxin in anesthetics and antibiotics: use of an ultrafiltration system and enzymatic LAL reaction.
An ultrafiltration system is used to obtain an endotoxin-free buffer for the LAL test. The procedure is combined with a kinetic LAL reaction of high sensitivity. This approach allows for the easy determination of endotoxin levels in parenterals with LAL-interfering substances, reducing the maximum valid dilution necessary for use in product development.
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D000777:Anesthetics / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis*; D008033:Limulus Test; D010288:Parenteral Nutrition; D014462:Ultrafiltration
D000777:Anesthetics; D000900:Anti-Bacterial Agents; D004731:Endotoxins