[Determination of cardenolides in Digitalis lanata by a solid-phase immunoenzyme method].
A competitive solid-phase EIA technique using high-affinity digoxin-specific monoclonal antibodies has been developed to determine cardenolides in Digitalis lanata. The test-system can detect digoxin and closely related compounds in concentration of 0.1 nmol/ml. The content of cardenolides, tested by ELISA, correlated well with the total content of digoxin, deslanoside C and lanatoside C as analysed by HPLC. The test-system is useful in estimating the productivity of large series and samples of tissue clones of Digitalis lanata.
Eremenko
S N
SN
Sukhov
I E
IE
Miroshnikov
A I
AI
D000911:Antibodies, Monoclonal; D002298:Cardenolides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004070:Digitalis / Q000737:chemistry*; D004077:Digoxin / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D010946:Plants, Medicinal; D010947:Plants, Toxic
D000911:Antibodies, Monoclonal; D002298:Cardenolides; D004077:Digoxin
Opredelenie kardenolidov v Digitalis lanata tverdofaznym immunofermentnym metodom.