title: Active increase in cardiolipin synthesis in the stationary growth phase and its physiological significance in Escherichia coli.
abstract: Activity of the Escherichia coli cardiolipin synthase, encoded by cls, increased about 10-fold in the stationary growth phase, while other committed-step enzymes in phospholipid biosynthesis rather decreased. A null cls mutant lost viability to 10(-4) of the wild-type cells during the prolonged incubation for 5 days. Cardiolipin was most stable among membrane phospholipids during the incubation. Accordingly, cardiolipin should play a role in survival of the cell and E. coli employs a sophisticated way to form cardiolipin according to need even under non-growing conditions.
authors.affiliation: Department of Biochemistry, Saitama University, Urawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hiraoka
authors.forename: H
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: I
authors.initials: I
authors.lastname: Shibuya
mesh_terms: D002308:Cardiolipins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008565:Membrane Proteins; D009154:Mutation; D010743:Phospholipids / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism
chemical_list: D002308:Cardiolipins; D008565:Membrane Proteins; D010743:Phospholipids; D017855:Transferases (Other Substituted Phosphate Groups); C100091:cardiolipin synthetase