title: Lectin binding studies with FITC-marked WGA and UEA I and flowcytometric measurements on isolated rat pancreatic acinar cells.
abstract: Lectin binding to the glycocalyx of isolated rat pancreatic acinar cells was studied by flowcytometric measurements. The pancreatic exocrine cells were prepared after collagenase digestion and cleaned in a density gradient. The fluorescence of cells was measured in a FACScan after binding of FITC marked WGA or UEA I. The binding of lectins was inhibited by preabsorption of WGA-FITC with N-acetyl-glucosamine, sialic acid or chitinous and by preabsorption of UEA-FITC with alpha-L-fucose, respectively. Furthermore, we were able to measure a decreased WGA-FITC and UEA-FITC binding after a short preincubation of isolated cells with the peptide hormone cholecystokinin and its agonists (caerulein, pentagastrin).
authors.affiliation: Medical Faculty, University of Rostock, FRG.
authors.forename: L
authors.initials: L
authors.lastname: Jonas
authors.forename: G
authors.initials: G
authors.lastname: Fulda
authors.forename: H
authors.initials: H
authors.lastname: Walzel
authors.forename: U
authors.initials: U
authors.lastname: Schulz
mesh_terms: D000818:Animals; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D010179:Pancreas / Q000166:cytology*; D051381:Rats; D014909:Wheat Germ Agglutinins
chemical_list: D014909:Wheat Germ Agglutinins; D016650:Fluorescein-5-isothiocyanate