title: Purification, kinetic properties and primary structure of bovine erythrocyte acylphosphatase.
abstract: An erythrocyte isoenzyme of acylphosphatase was purified from bovine red cells. The protein was characterized as regards the kinetic parameters and amino acid sequence. A simple and rapid sequencing strategy, based on a few experiments, was used for reconstructing the primary structure of the enzyme, since the purification procedure gave a very low yield. The length of the polypeptide chain is 100 residues. Comparison with the analogous human isoenzyme indicates that the primary structure is about 90% conserved. The presence of two additional residues at the acetylated N-terminus confirms the hypervariability for this region found in other acylphosphatases.
authors.affiliation: Dipartimento di Scienze Biochimiche, Università di Firenze, Italia.
authors.forename: L
authors.initials: L
authors.lastname: Pazzagli
authors.forename: U K
authors.initials: UK
authors.lastname: Ikram
authors.forename: G
authors.initials: G
authors.lastname: Liguri
authors.forename: N
authors.initials: N
authors.lastname: Taddei
authors.forename: A
authors.initials: A
authors.lastname: Gentilini
authors.forename: C
authors.initials: C
authors.lastname: Cecchi
authors.forename: G
authors.initials: G
authors.lastname: Manao
authors.forename: G
authors.initials: G
authors.lastname: Cappugi
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000097:blood* / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D017124:Conserved Sequence; D003488:Cyanogen Bromide; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000737:chemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D000097024:Acylphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D003488:Cyanogen Bromide; D007527:Isoenzymes; D010446:Peptide Fragments; D000097024:Acylphosphatase