[A lysoamidase bacteriolytic complex. 1. Determination of the nature of interaction of enzymes and the polysaccharide, included in the complex].
The polysaccharide component of the bacteriolytic enzymatic complex "lysoamidase" (M(r) 1300 kDa) has been found to contain negatively charged ionic groups and can electrostatically interact with some enzymes within the complex. At pH 8.0 optimal for bacterial lysis, such interaction promotes the stabilization of bacteriolytic enzymes, increasing their half-inactivation temperature by 8 degrees and their stability upon storage by 40%. The polysaccharide decreases the thermal stability of phosphatase but has no stabilizing effect on the metalloprotease.
Stepnaia
O A
OA
Ledova
L A
LA
Kulaev
I S
IS
D000890:Anti-Infective Agents / Q000494:pharmacology*; D004560:Electricity; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D006358:Hot Temperature; D010447:Peptide Hydrolases / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*
D000890:Anti-Infective Agents; D004791:Enzyme Inhibitors; D011134:Polysaccharides; C060332:lysoamidase; D010744:Phosphoric Monoester Hydrolases; D010447:Peptide Hydrolases
Bakterioliticheskiĭ kompleks lizoamidaza. 1. Opredelenie prirody vzaimodeĭstviia fermentov i polisakharida, vkhodiashchikh v sostav kompleksa.