Purification and characterization of a cell growth factor from a human leukemia cell line: immunological identity with ferritin.
We have succeeded in long-term cultivation of a human erythroleukemia cell line, K-562-T1 (T. Okabe, M. Fujisawa, and F. Takaku, Proc. Natl. Acad. Sci. USA, 81: 453-455, 1984). The cells grown in a protein-free chemically defined medium have been shown to produce cell growth factors (A. Mihara et al., In Vitro Cell. Dev. Biol., 23: 317-322, 1987). In this study, we have purified a cell growth factor from the conditioned medium that stimulates the proliferation of human leukemia cells, HL-60. In the purified factor, two major protein bands of 24 kDa and 22 kDa were identified on a sodium dodecyl sulfate-polyacrylamide gel. The 22 kDa protein was stained with a monoclonal antibody to the light chain of ferritin. The growth-promoting activity of the purified factor was coprecipitated with a monoclonal antibody to the light chain or heavy chain of human ferritin. These results suggest that K-562-T1 cells produce a cell growth factor that is related to ferritin.
Kikyo
N
N
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Suda
M
M
Kikyo
N
N
Hagiwara
K
K
Yasukawa
K
K
Fujisawa
M
M
Yazaki
Y
Y
Okabe
T
T
D017077:Culture Media, Conditioned; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000302:isolation & purification*; D006133:Growth Substances / Q000302:isolation & purification*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D006133:Growth Substances; D005293:Ferritins