Acceleration of natural killer (NK) cell recovery by a glucan, sizofiran, in anti-asialoGM1 antibody-treated mice.
The proportion of asialoGM1 positive cells and NK activity of murine splenic cells was reduced to almost zero one day after intravenous injection of rabbit anti-asialoGM1 antibody. The cells and the activity started to increase at the latest 3 days after the injection, although the proportion was far below that of the normal control. The proportion of asialoGM1 positive cells and NK activity increased more remarkably when 1,3-beta glucan, sizofiran, was administered intramuscularly one day after the antibody injection. The increases were dose related (50-1000 micrograms/mouse). The fact that sizofiran hastened the recoveries of splenic NK activity and asialoGM1 positive cells suggests sizofiran may have the activity to accelerate the differentiation of asialoGM1 positive NK cells.
Tsuchiya
Y
Y
Central Research Laboratory, Kaken Pharmaceutical Co., Ltd., Kyoto, Japan.
Yang
Z B
ZB
Nakahashi
S
S
Arika
T
T
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D001854:Bone Marrow Cells; D003601:Cytotoxicity Tests, Immunologic; D005472:Fluorouracil / Q000494:pharmacology; D005677:G(M1) Ganglioside / Q000276:immunology*; D005936:Glucans / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D011817:Rabbits; D012566:Sizofiran / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D005936:Glucans; D007377:Interleukin-3; D015850:Interleukin-6; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D012566:Sizofiran; D005472:Fluorouracil