Adhesive interactions of neutrophils and leukotriene synthesis.
Cell-substrate and cell-cell adhesion of neutrophils has been found to slow down the calcium ionophore A23187-induced synthesis of 5-lipoxygenase (5-LO) metabolites of arachidonic acid. Addition of the exogenous substrate, arachidonic acid (AA), together with A23187, resulted in the enhanced production of leukotriene B4 (LTB4) and 5-hydroxyeicosatetraenoic acid (5-HETE) by adherent neutrophils in comparison with those by the cells in suspension. We observed also the enhanced production of 5-LO metabolites in attached cells when we stimulated the cells by the combined action of phorbol 12-myristate 13-acetate (PMA) and A23187. Thus, the adhesion to solid substrate and to other cells, an important regulatory factor for the activity of many cells, is a powerful regulator of leukotriene production by neutrophils.
Sud'ina
G F
GF
A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
Galkina
S I
SI
Barsky
O A
OA
Margolis
L B
LB
D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D016718:Arachidonic Acid / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002448:Cell Adhesion; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000096:biosynthesis*; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007975:Leukotriene B4; D016718:Arachidonic Acid; D000001:Calcimycin; D001094:Arachidonate 5-Lipoxygenase; D013755:Tetradecanoylphorbol Acetate