title: Constitutive and inducible expression of PDGF in the human basophilic cell line, KU 812.
abstract: The human basophilic cell line KU 812, that also has some mast cell characteristics, was found to express the PDGF-A gene and secrete PDGF-A like activity. After treatment with IL-6+ TNF-alpha, the PDGF-A mRNA expression increased as did cytoplasmic immunostaining with anti-PDGF antibodies. Secretion of PDGF-A was visualized by immunoprecipitation. An augmentation of non-secreted PDGF-like activity after IL-6+ TNF-alpha treatment was not accompanied by induction of the long splice variant of the PDGF-A-chain mRNA. Treatment with TPA caused an increase in PDGF-A expression and in addition, an induction of PDGF-B transcripts were seen. Staining of cytospin preparations with anti-PDGF antibodies visualized a substantial increase in immunostaining of the TPA treated cells and both intracellular and secreted PDGF-AA-like activity was substantially increased as compared to untreated control cultures. There was a concomitant induction of exon 6 specific mRNA, corresponding to a cellular retention signal after TPA treatment. Our results show that PDGF can be produced by a cell line of the basophilic/mast cell lineage, i.e. cells involved in allergic disorders and inflammation.
authors.affiliation: Department of Pathology, University Hospital, Uppsala, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Forsberg
authors.forename: G
authors.initials: G
authors.lastname: Nilsson
authors.forename: Z P
authors.initials: ZP
authors.lastname: Ren
authors.forename: L
authors.initials: L
authors.lastname: Hellman
authors.forename: B
authors.initials: B
authors.lastname: Westermark
authors.forename: M
authors.initials: M
authors.lastname: Nistér
mesh_terms: D001491:Basophils / Q000166:cytology / Q000378:metabolism*; D015152:Blotting, Northern; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media; D005786:Gene Expression Regulation; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D011233:Precipitin Tests; D012326:RNA Splicing; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism
chemical_list: D003470:Culture Media; D010982:Platelet-Derived Growth Factor; D017479:Receptors, Platelet-Derived Growth Factor