Effect of sorbent-based dialytic therapy with the BioLogic-DT on an experimental model of hepatic failure.
An experimental model of hepatic failure in the dog has been developed in which the liver is devascularized in two stages. Under general anesthesia, a portacaval shunt is created, ligatures placed around the hepatic and gastroduodenal arteries, and the dog recovered. Two days later under general anesthesia, the ligatures are pulled, converting hepatic insufficiency to hepatic failure. Five control animals developed hypotension, severe lactic acidosis, hypoglycemia, and increasing liver enzyme levels during 6 hrs of follow-up. The BioLogic-DT system includes a cellulosic plate dialyzer with a suspension of powdered charcoal and cation exchangers as dialysate. Five animals were treated with the BioLogic-DT for 6 hrs after creation of hepatic failure. These animals were more stable physiologically, developed less lactic acidosis and less enzyme elevation, and maintained high normal blood glucose levels. The results help explain the clinical improvement demonstrated in patients with hepatic failure treated by the BioLogic-DT, and confirm that many of the toxins of hepatic failure are dialyzable and bound by simple sorbents such as charcoal and cation exchangers.
Ash
S R
SR
Hemocleanse, Inc., Purdue Research Park, W. Lafayette, Indiana 47906.
Carr
D J
DJ
Blake
D E
DE
Rainier
J B
JB
Demetriou
A A
AA
Rozga
J
J
D000641:Ammonia / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001639:Bicarbonates / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004285:Dogs; D006501:Hepatic Encephalopathy / Q000201:enzymology / Q000628:therapy*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D007958:Leukocyte Count; D010976:Platelet Count; D011188:Potassium / Q000097:blood; D006435:Renal Dialysis / Q000295:instrumentation*; D016060:Sorption Detoxification / Q000295:instrumentation*
D001639:Bicarbonates; D001786:Blood Glucose; D007773:Lactates; D019344:Lactic Acid; D000641:Ammonia; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D011188:Potassium