title: Regional localization of the lactase-phlorizin hydrolase gene, LCT, to chromosome 2q21.
abstract: The gene LCT which codes for the intestinal disaccharidase lactase-phlorizin hydrolase has previously been mapped, using somatic cell hybrids and linkage analysis, using the CEPH families, to chromosome 2. We describe here the regional localization of LCT to chromosome 2q21 by polymerase chain reaction (PCR) analysis of somatic cell hybrids and in situ hybridization. LCT is closely linked to D2S44, with a lod score of 30.6 at theta = 0.10.
authors.affiliation: MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London.
authors.forename: C B
authors.initials: CB
authors.lastname: Harvey
authors.forename: M F
authors.initials: MF
authors.lastname: Fox
authors.forename: P A
authors.initials: PA
authors.lastname: Jeggo
authors.forename: N
authors.initials: N
authors.lastname: Mantei
authors.forename: S
authors.initials: S
authors.lastname: Povey
authors.forename: D M
authors.initials: DM
authors.lastname: Swallow
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D017931:DNA Primers; D005091:Exons; D005260:Female; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D010694:Lactase-Phlorizin Hydrolase / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012377:Rodentia
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D010694:Lactase-Phlorizin Hydrolase