title: Effects of pyrantel pamoate on adult and preadult Toxocara canis worms: an electron microscope and autoradiography study.
abstract: Adult as well as preadult Toxocara canis isolated from the intestine of a beagle were incubated for 2, 4, and 14 h in medium containing either different concentrations of pyrantel pamoate (23.6, 236, and 2360 micrograms/ml medium) or tritiated pyrantel pamoate (2.36 micrograms/ml medium). These incubations were performed to study the effects of pyrantel pamoate on the morphology of the parasitic nematodes and to obtain information concerning the mode of uptake, the distribution, and the total amount of pyrantel pamoate ingested by T. canis. The results of the ultrastructure studies indicate that the intestine, hypodermis, and muscle cells are the organs that are predominantly affected by the drug. Additionally, it turned out that the duration of the treatment, i.e., the incubation time, was more important in determining the efficacy of pyrantel pamoate against T. canis than was the concentration itself. Autoradiography studies revealed that the adult worms ingest the drug orally, whereas preadults absorb pyrantel pamoate mainly through the whole body surface. Finally, measurements of the total amount of pyrantel pamoate taken up by T. canis indicated that adult worms can limit or even reduce the ingestion of pyrantel for more than 4 h, but then ingest large amounts of the drug. Preadult worms, however, absorb the drug more or less continuously during the first 14 h through the cuticula, albeit in lower concentrations than the adults. The different experiments elucidate differences in the uptake of pyrantel pamoate as well as in the total amount of drug ingested or absorbed by adult or preadult worms, leading to the assumption that repeated treatment with lower concentrations will be more effective than high concentrations given only once.
authors.affiliation: Ruhr University Bochum, Department of Parasitology, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Mackenstedt
authors.forename: S
authors.initials: S
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Mehlhorn
authors.forename: M
authors.initials: M
authors.lastname: Stoye
authors.forename: W
authors.initials: W
authors.lastname: Traeder
mesh_terms: D000375:Aging; D000818:Animals; D004285:Dogs / Q000469:parasitology; D004305:Dose-Response Relationship, Drug; D005260:Female; D007422:Intestines / Q000187:drug effects / Q000469:parasitology / Q000648:ultrastructure; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D009132:Muscles / Q000187:drug effects / Q000648:ultrastructure; D010053:Ovary / Q000187:drug effects / Q000648:ultrastructure; D011716:Pyrantel Pamoate / Q000032:analysis / Q000494:pharmacology*; D013090:Spermatocytes / Q000187:drug effects / Q000648:ultrastructure; D013997:Time Factors; D017166:Toxocara canis / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D011716:Pyrantel Pamoate