Modifying effect of 26-hydroxycholesterol on low-magnesium-induced atherosclerotic changes in the cultured human arterial smooth muscle cell.
This study investigated the modifying effect of 26-hydroxycholesterol (26-OHC) on low-Mg(2+)-induced atherosclerotic changes in human arterial smooth muscle cells. Cells were exposed to a medium containing a concentration of 142, 332, 665 and 949 microM Mg2+ (as control) for the designed periods. Half the cells were simultaneously exposed to 10 micrograms/ml of 26-OHC. The results showed that the cytotoxicity of low Mg2+ and the stimulating effect of low Mg2+ on cytosolic free Ca2+ level and 45Ca2+ uptake were accentuated by 26 microns OHC. The significant increase of [3H]thymidine incorporation by Mg2+ level at and lower than 332 was, however, counteracted by 26-OHC. While an increased [3H]leucine incorporation occurred only at a level of 665 microM Mg2+, an additional increase of [3H]leucine incorporation also occurred at a level of 142 microM Mg2+ in the presence of 26-OHC. We suggest that 26-OHC could modify the atherogenic effect of low Mg2+.
Zhou
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Burnsides Research Laboratory, University of Illinois, Urbana.
Kummerow
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D001161:Arteriosclerosis / Q000473:pathology*; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008275:Magnesium Deficiency / Q000473:pathology*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology*; D011247:Pregnancy; D013936:Thymidine / Q000378:metabolism
D002132:Calcium Radioisotopes; D003470:Culture Media; D006888:Hydroxycholesterols; C007042:cholest-5-ene-3 beta,26-diol; D007930:Leucine; D002118:Calcium; D013936:Thymidine