title: [Estimation of nitrogen-corrected metabolizable energy value of laboratory canine diets].
abstract: This study evaluates the reliability of the equation, metabolizable energy (ME) = digestible energy (DE) - 1.25 x digestible crude protein (DCP), as a means of estimating the energy value of non-purified diets for laboratory dogs. To do this, five digestion and nitrogen-balance (NB) trials and determination of the energy value of a diet were conducted using five diets and four mature, male Beagles for each experiment. The resulting digestibility of crude protein, acid ether extract, and nitrogen-free extract of these diets were 79.5%, 91.6%, and 84.8%, respectively. The NB in every diet was positive: 1.4-8.6 mg/g dry matter of ingested diet. Of the nitrogen (N) ingested, 21% was excreted into feces, 66% was excreted into urine, and 13% was retained. In terms of dietary gross energy (GE), 18% was lost in feces and 5% was excreted into urine leaving 77% as ME. Retained energy in the body was only 1%, and N-corrected metabolizable energy (MEn) was 76% of GE. The average urinary energy (UE) loss per gram of urinary N was 7.84kcal. The correlation between UE/DCP and NB values is statistically significant: UE/DCP = 1.24-0.03NB, r = 0.798, P < 0.001. Therefore, UE/DCP is 1.24kcal/g at N-equilibrium, almost the same as the factor in the above equation. The ME value calculated by the equation is identical to the MEn value and both are significantly lower (P < 0.05) than the empirically determined ME value.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Tsukuba Research Branch, Nihon Nosan Kogyo K.K., Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohshima
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuma
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Abe
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000830:Animals, Laboratory; D004063:Digestion; D004285:Dogs / Q000378:metabolism* / Q000502:physiology; D004734:Energy Metabolism; D008297:Male; D009584:Nitrogen / Q000378:metabolism*
chemical_list: D009584:Nitrogen