title: Smooth muscle myosin heavy chain locus (MYH11) maps to 16p13.13-p13.12 and establishes a new region of conserved synteny between human 16p and mouse 16.
abstract: The human smooth muscle myosin heavy chain locus (MYH11) was mapped by fluorescence in situ hybridization to the middle of the p arm of chromosome 16 using a genomic cosmid clone containing coding sequences of the gene as probe. Probe from coding sequence, when applied to Southern blots of a panel of hybrids containing different portions of human chromosome 16, localized the gene to 16p13.13-13.12. Coding sequence PCR primers, when used on the DNA from a CHO-mouse hybrid clone mapping panel informative for mouse chromosomes, showed that the gene was located on mouse chromosome 16. These results correct a recent assignment of MYH11 from 16q12.2 to the region of the 16p-arm inversion breakpoint seen in acute myelomonocytic leukemia (AMML) M4Eo and demonstrate that the conflicting data do not result from the presence of additional MYH genes on the q arm of the chromosome. Also, a new region of conserved synteny between human 16p and mouse 16 is established.
authors.affiliation: Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
authors.forename: Z
authors.initials: Z
authors.lastname: Deng
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Marlton
authors.forename: D F
authors.initials: DF
authors.lastname: Claxton
authors.forename: S
authors.initials: S
authors.lastname: Lane
authors.forename: D F
authors.initials: DF
authors.lastname: Callen
authors.forename: F S
authors.initials: FS
authors.lastname: Collins
authors.forename: M J
authors.initials: MJ
authors.lastname: Siciliano
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D017931:DNA Primers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000378:metabolism*; D009218:Myosins / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D009218:Myosins