title: Amino acid losses in ileostomy fluid on a protein-free diet.
abstract: The contribution of losses via the gastrointestinal tract to maintenance amino acid requirements was assessed by collecting the ileostomy fluid of volunteers given a protein-free diet for 5 d. The subjects were eight adult men and women with terminal ileum ileostomies after ulcerative colitis. Four consecutive 24-h collections of both digesta and urine were made. On the last 2 d an antibiotic was given that suppressed microbial activity in the digesta and slightly reduced ileostomy outflow. Mean daily amino acid excretion in ileostomy fluid ranged from 32 mg/d for methionine to approximately 330 mg/d for aspartate and glutamate. These losses were compared with current international estimates of amino acid requirements. For most essential amino acids gastrointestinal losses accounted for 14-33% of daily maintenance requirements but for threonine the contribution was 61%.
authors.affiliation: Rowett Research Institute, Bucksburn, Aberdeen, UK.
authors.forename: M F
authors.initials: MF
authors.lastname: Fuller
authors.forename: A
authors.initials: A
authors.lastname: Milne
authors.forename: C I
authors.initials: CI
authors.lastname: Harris
authors.forename: T M
authors.initials: TM
authors.lastname: Reid
authors.forename: R
authors.initials: R
authors.lastname: Keenan
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001826:Body Fluids / Q000378:metabolism; D004044:Dietary Proteins / Q000008:administration & dosage*; D004064:Digestive System / Q000378:metabolism; D005260:Female; D006801:Humans; D007081:Ileostomy; D008297:Male; D008875:Middle Aged
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins