Cell lines derived from ultraviolet radiation-induced benign melanocytic nevi in Monodelphis domestica exhibit cytogenetic aneuploidy.
The gray short-tailed opossum, Monodelphis domestica, develops dermal melanocytic nevi (MN) after long-term chronic exposure to UVB (midwavelength ultraviolet radiation) alone. We developed cell lines from six UVB-induced dermal benign melanocytic lesion biopsies. One of the MN was determined histologically to be a benign melanoma (BM), whereas the remainder were benign melanocytic hyperplasias (MH). The cell lines were not tumorigenic when injected subcutaneously into athymic nude mice. Protein extracts prepared from these cell lines were analyzed electrophoretically on polyacrylamide gels and protease zymograms in preliminary attempts to identify protein and protease markers for pathogenesis. Cytogenetic analyses showed that half (three of six) of the MN cell lines exhibited aneuploidy involving extra copies of chromosomes 3, 5, 7, and/or 8. This result suggests that nonrandom aneuploidy can be an early event in chronic UVB induction of benign dermal melanocytic lesions. Karyotyping also showed a centromeric variant of chromosome 7 in some animals, which was confirmed to be constitutional. These Monodelphis cell lines will be valuable reagents for future studies of UVB-induced damage to mammalian skin.
Dooley
T P
TP
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228.
Mattern
V L
VL
Moore
C M
CM
Porter
P A
PA
Robinson
E S
ES
VandeBerg
J L
JL
D000782:Aneuploidy; D000818:Animals; D004195:Disease Models, Animal; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000032:analysis; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D009508:Nevus, Pigmented / Q000209:etiology / Q000235:genetics*; D009893:Opossums; D012878:Skin Neoplasms / Q000209:etiology / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays / Q000009:adverse effects*
D009363:Neoplasm Proteins