Natural autoantibodies cross-react with a peptide derived from the second conserved region of HIV-1 envelope glycoprotein gp120.
It was recently shown that peptide NTM (RSANFTDNAKTIIVQLNESV), corresponding to residues 280-299 in the second conserved domain of HIV-1 envelope glycoprotein gp120, has spectral and sequence similarity with human vasoactive intestinal peptide, VIP (Veljkovic et al., Biochem. Biophys. Res. Commun., 189, 705-710, 1992). We found that natural autoantibodies cross-reactive with this peptide can be detected in sera from HIV-negative asthma patients and healthy blood donors. The level of these antibodies is significantly higher in asthma patients than in healthy individuals, suggesting that these antibodies can in fact be at least partly identical to natural anti-VIP antibodies previously described (Paul et al., Biochem. Biophys. Res. Commun., 130, 479-483, 1985; Paul et al., Science, 244, 158-1162, 1989). Possible origin and role of these antibodies in AIDS pathogenesis and therapy are discussed.
Veliković
V
V
Laboratory for Multidisciplinary Research 180/2, Institute of Nuclear Sciences, VINCA, Beograd, Yugoslavia.
Metlas
R
R
Danilo
V
V
Cavor
L
L
Pejinović
N
N
Dujuć
A
A
Zakhariev
S
S
Guarnaccia
C
C
Pongor
S
S
D000328:Adult; D000595:Amino Acid Sequence; D001249:Asthma / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000378:metabolism*; D017124:Conserved Sequence; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D014660:Vasoactive Intestinal Peptide / Q000737:chemistry
D001323:Autoantibodies; D015699:HIV Envelope Protein gp120; D010446:Peptide Fragments; D010455:Peptides; D014660:Vasoactive Intestinal Peptide