Elevated content of phospholipase C-gamma 1 in colorectal cancer tissues.
BACKGROUND
Phospholipase C isozymes (PLC) play a role in ligand-mediated signal transduction for cellular activity, such as proliferation and differentiation. However, the biologic significance of their molecules in carcinogenesis or tumor progression is yet to be determined.


METHODS
Using PLC isozyme-specific antibodies, the relative content of PLC in human colorectal carcinomas and in normal colonic mucosa was examined.


RESULTS
Immunoreactive analysis revealed considerably higher levels of PLC-gamma 1 protein in 15 of 17 colorectal carcinomas and little difference in PLC-beta 1 or PLC-gamma 1 content compared with normal colorectal tissues. By radioimmunoassay and Western blotting, PLC-gamma 1 showed three-fold to fourfold more expression in carcinomas than that in normal tissues.


CONCLUSIONS
Although factors that might influence the level of PLC-gamma 1 expression in colorectal carcinomas still remain obscure, the fact that most colorectal carcinomas display elevated levels of PLC-gamma 1 expression implies that PLC-gamma 1 may play an important role in proliferation of colorectal carcinoma cells.
Noh
D Y
DY
Laboratory of Cell Biology, Seoul National University College of Medicine, Korea.
Lee
Y H
YH
Kim
S S
SS
Kim
Y I
YI
Ryu
S H
SH
Suh
P G
PG
Park
J G
JG
D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D003106:Colon / Q000737:chemistry / Q000473:pathology; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry / Q000473:pathology; D007527:Isoenzymes / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D054799:Phospholipase C beta; D054803:Phospholipase C delta; D051966:Phospholipase C gamma; D012004:Rectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D015398:Signal Transduction / Q000502:physiology; D010738:Type C Phospholipases / Q000032:analysis* / Q000235:genetics
D007527:Isoenzymes; D010738:Type C Phospholipases; D054799:Phospholipase C beta; D054803:Phospholipase C delta; D051966:Phospholipase C gamma