title: Capsid assembly and involved function analysis of twelve core protein mutants of duck hepatitis B virus.
abstract: The roles of different regions of the duck hepatitis B virus (DHBV) core protein on viral capsid assembly and related functions were examined. Twelve deletion and insertion mutations which covered 80% of the DHBV C open reading frame were constructed and expressed in Escherichia coli. The N-terminal region (amino acids 3 to 66) of DHBV core protein was important for its tertiary structure and function in E. coli. The expressed core mutants without this region apparently inhibited E. coli growth. The results of transmission electron microscopy of E. coli thin sections, capsid agarose gel, and sucrose gradient sedimentation demonstrated that a few DHBV core mutants with insertion in the N terminus and deletion in the C terminus retained the ability to form core-like particles in E. coli. However, other mutations in most of N-terminal and central regions strongly inhibited the self-assembly ability of DHBV core protein in E. coli. In addition, the mutant with a C-terminal region deletion (amino acids 181 to 228) lost most of the nucleic acid-binding activity of the DHBV core protein.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Tiantan, Beijing.
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: Z
authors.initials: Z
authors.lastname: Ying
authors.forename: S
authors.initials: S
authors.lastname: Hua
authors.forename: W
authors.initials: W
authors.lastname: Dong
authors.forename: H
authors.initials: H
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000378:metabolism*; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000648:ultrastructure; D005814:Genes, Viral / Q000235:genetics; D015611:Hepatitis B Virus, Duck / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D014758:Viral Core Proteins / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D011994:Recombinant Proteins; D014758:Viral Core Proteins