title: Production and properties of recombinant C3-type phosphoenolpyruvate carboxylase from Sorghum vulgare: in vitro phosphorylation by leaf and root PyrPC protein serine kinases.
abstract: In this work, the C3-type form of Sorghum phosphoenolpyruvate carboxylase (PyrPC) was produced in PyrPC-deficient strains of Escherichia coli transformed by a plasmid bearing the corresponding full-length cDNA (CPR1). The full-sized protein was purified to homogeneity by immunoaffinity chromatography. Some functional and regulatory properties were described; notably, the immunopurified PyrPC could be phosphorylated in reconstituted assay by 1) both a mammalian PKA and the PyrPC protein serine kinase purified from Sorghum leaves and 2) a novel protein kinase affinity-purified from Sorghum roots. In all cases phosphorylation was accompanied by a marked reduction in its malate sensitivity.
authors.affiliation: Laboratoire de Physiologie Végétale Moléculaire, Unité de Recherche Associée CNRS D-1128, Université de Paris-Sud, Orsay, France.
authors.forename: V
authors.initials: V
authors.lastname: Pacquit
authors.forename: S
authors.initials: S
authors.lastname: Santi
authors.forename: C
authors.initials: C
authors.lastname: Cretin
authors.forename: V L
authors.initials: VL
authors.lastname: Bui
authors.forename: J
authors.initials: J
authors.lastname: Vidal
authors.forename: P
authors.initials: P
authors.lastname: Gadal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D007527:Isoenzymes / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008322:Mammals; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010730:Phosphoenolpyruvate Carboxylase / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D006109:Poaceae / Q000201:enzymology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D011994:Recombinant Proteins; D017346:Protein Serine-Threonine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D010730:Phosphoenolpyruvate Carboxylase