Effect of catalytically active recruited crystallins on lens metabolism.
The impact on duck lens metabolism of recruited epsilon-crystallin/LDH-B4 and tau-crystallin/enolase was investigated by NMR spectroscopy. A comparison of the duck lens metabolite profile with that of the calf in which recruited crystallins are absent revealed significant increases in ATP, alpha-glycerophosphate (alpha-GP) and pyridine dinucleotide concentrations. The alterations in the concentrations of ATP and alpha-GP appear to be related to both the high concentration of NAD and the elevated reduced to oxidized NADH/NAD+ ratio.
Reddy
M C
MC
Department of Physiology, New Jersey Medical School, Newark 07103.
Groth-Vasselli
B
B
Sharon
A
A
Farnsworth
P N
PN
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002417:Cattle; D003459:Crystallins / Q000378:metabolism*; D004372:Ducks; D005994:Glycerophosphates / Q000378:metabolism; D066298:In Vitro Techniques; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009243:NAD / Q000378:metabolism
D003459:Crystallins; D005994:Glycerophosphates; D007527:Isoenzymes; D009243:NAD; D000255:Adenosine Triphosphate; C029620:alpha-glycerophosphoric acid; D007770:L-Lactate Dehydrogenase