Gastric emptying of radionuclide-labeled solid meal in beagle dogs using a scintigraphic method.
A scintigraphic method for in vivo radiotracers measurement is described to investigate the gastrointestinal emptying rate in beagle dogs. The proposed method has been applied to study the effect of a pharmacological compound in enhancing stomach emptying and involves the following steps: 1) 131I-labeled anion exchange resin grains preparation, as solid food markers to be ingested by the dogs; 2) continuous recording of gastric emptying evolution over a period of 5 h with fast data-gathering instrumentation employing small size and light-weight CsI scintillation detectors coupled to a photodiode, equipped with a lead shield and collimator; 3) comparison of results obtained with drugs to previous standard emptying curves. The purpose of this study was to evaluate the prokinetic activity of a recent synthesized compound (BIMU 1).
Terrani
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Department of Nuclear Engineering, Istituto CESNEF, Politecnico di Milano, Italy.
Pacenti
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Schiavone
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D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology*; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D020117:Cisapride; D004285:Dogs; D004435:Eating; D005746:Gastric Emptying / Q000187:drug effects; D007457:Iodine Radioisotopes; D010880:Piperidines / Q000494:pharmacology; D011985:Receptors, Serotonin / Q000502:physiology; D012588:Scintillation Counting / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
D000897:Anti-Ulcer Agents; D001562:Benzimidazoles; D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D007457:Iodine Radioisotopes; D010880:Piperidines; D011985:Receptors, Serotonin; C070187:(endo-N-8-methyl-8-azabicyclo(3.2.1)oct-3-yl)-2,3-dihydro-3-ethyl-2-oxo-1H-benzimidazol-1-carboxamide; D020117:Cisapride