Chlamydia latex agglutination antigen and protocol improvement and psittacine bird anti-chlamydial immunoglobulin reactivity.
Latex agglutination detection of chlamydial antibody activity in psittacine bird sera was significantly more sensitive when an improved protocol was followed than was a test using the previously used protocol. Titers of antibody-positive sera were fourfold to 32-fold higher by the improved protocol than titers by the previously used protocol, whereas antibody-negative sera were negative by both protocols. Column chromatography was used to separate immunoglobulins in psittacine bird serum. Immunoglobulin M was reactive in latex agglutination (LA) but non-reactive in direct complement fixation (DCF). Immunoglobulin G was reactive in LA and DCF. The fifth supernatant fluid of serum multiply absorbed with latex beads was non-reactive in LA and DCF. The immunoglobulins reactive in LA had high avidity and affinity for latex beads. Prolonged storage at 4 C to 6 C preserved LA reactivity of absorbed immunoglobulins better than storage at 21 C to 23 C.
Grimes
J E
JE
Texas Veterinary Medical Diagnostic Laboratory, College Station 77841-3040.
Phalen
D N
DN
Arizmendi
F
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001715:Bird Diseases / Q000175:diagnosis / Q000276:immunology; D001717:Birds / Q000276:immunology*; D002689:Chlamydia / Q000276:immunology*; D002690:Chlamydia Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D005069:Evaluation Studies as Topic; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000097:blood / Q000302:isolation & purification; D007841:Latex Fixation Tests / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M