title: Albumin-binding proteins on the surface of the Streptococcus milleri group and characterization of the albumin receptor of Streptococcus intermedius C5.
abstract: Members of the Streptococcus milleri group (SMG) that react with Lancefield group C antisera were shown to bind large amounts of albumin although there was no direct relation between these two properties as polyclonal antisera to Lancefield group C antigen did not prevent the binding of albumin. There was a specificity for albumin binding, with albumin from man, monkeys, cat, dog and mouse being bound to a greater degree than albumin from cow, horse, goat or rabbit. Gold-labelled albumin was shown to be located close to the surface of strains by transmission electron microscopy. A cell-surface protein of M(r) 24,000, which was liberated by lysozyme treatment of cells, was shown to be the cell-surface receptor on Streptococcus intermedius C5. The receptor was physically dissimilar from protein G, an albumin- and IgG-binding protein of 'large-colony' Lancefield group C and G streptococci.
authors.affiliation: Institute of Dental Research, United Dental Hospital, Sydney, NSW, Australia.
authors.forename: M D
authors.initials: MD
authors.lastname: Willcox
authors.forename: M
authors.initials: M
authors.lastname: Patrikakis
authors.forename: C Y
authors.initials: CY
authors.lastname: Loo
authors.forename: K W
authors.initials: KW
authors.lastname: Knox
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D001483:Base Sequence; D002415:Cats; D002645:Chickens; D006224:Cricetinae; D004285:Dogs; D006041:Goats; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D006868:Hydrolysis; D008253:Macaca mulatta; D051379:Mice; D008969:Molecular Sequence Data; D010215:Papio; D010527:Perissodactyla; D011817:Rabbits; D051381:Rats; D017457:Receptors, Albumin / Q000235:genetics / Q000302:isolation & purification*; D012709:Serum Albumin / Q000737:chemistry; D013045:Species Specificity; D013291:Streptococcus / Q000737:chemistry* / Q000276:immunology; D013552:Swine
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D017457:Receptors, Albumin; D012709:Serum Albumin