Vasopressin mRNA in the cerebellum and circumventricular organs: a quantitative in situ hybridization study.
To answer the question as to whether vasopressin is synthesized in brain structures other than the supraoptic and paraventricular nuclei of the hypothalamus, vasopressin mRNA was detected by in situ hybridization in the pituitary, cerebellum, dentate gyrus, habenula and circumventricular organs. The highest levels (0.3-0.2 pmol/g), measured by quantitative autoradiography, were observed in the pituitary intermediate lobe and the granular layers of the cerebellum and dentate gyrus. Lower levels (0.15-0.08 pmol/g) were found in the medial habenula, adenohypophysis, area postrema, pineal, subfornical and subcommissural organs.
Lepetit
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INSERM CJF 90-10, CNRS URA 1195, Laboratoire d'Anatomie Pathologique, Faculté de Médecine Alexis Carrel, Lyon, France.
Fevre-Montange
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M
Gay
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N
Belin
M F
MF
Bobillier
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P
D000818:Animals; D001345:Autoradiography; D002531:Cerebellum / Q000378:metabolism*; D002552:Cerebral Ventricles / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D015345:Oligonucleotide Probes; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014667:Vasopressins / Q000096:biosynthesis*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014667:Vasopressins