title: Escherichia coli: derived murine interleukin-1 beta with N-terminus partially N alpha-acetylated.
abstract: Two forms of murine recombinant interleukin-1 beta (mrIL-1 beta) from Escherichia coli were purified by ion exchange column chromatography; each exhibited equivalent biological activity in the murine thymocyte proliferation assay. It was determined by mass spectrometry of tryptic peptides that both retained the initiating methionine but one form was N alpha-acetylated at the N-terminus.
authors.affiliation: Du Pont Merck Pharmaceutical Company, Glenolden Laboratory, Pennsylvania 19036.
authors.forename: M A
authors.initials: MA
authors.lastname: Lischwe
authors.forename: R C
authors.initials: RC
authors.lastname: Newton
authors.forename: J J
authors.initials: JJ
authors.lastname: Huang
authors.forename: R A
authors.initials: RA
authors.lastname: Yates
authors.forename: L A
authors.initials: LA
authors.lastname: Breth
authors.forename: B S
authors.initials: BS
authors.lastname: Larsen
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli; D007375:Interleukin-1 / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D011993:Recombinant Fusion Proteins