title: Solid-phase extraction and gas chromatographic-mass spectrometric determination after hydrolysis of 2-aminofluorene haemoglobin adducts in blood of rats.
abstract: 2-Nitrofluorene is an environmental pollutant that binds covalently to haemoglobin after nitroreduction and successive N-hydroxylation. these haemoglobin adducts can be cleaved in vitro by mild base-catalysed hydrolysis. For the enrichment of arylamines from the aqueous hydrolysate, an extraction procedure with an organic solvent is widely used. Because of the formation of a thick emulsion layer between the aqueous and organic solvent layers, the extraction is laborious and inefficient. The use of Amberlite XAD2 provides a simple extraction procedure yielding a recovery of ca. 70%. Calibration curves in haemoglobin solution were prepared with a correlation coefficient of 0.998 (n = 12). The inter-day coefficient of variation amounted to 14%.
authors.affiliation: Department of Toxicology, University of Nijmegen, Netherlands.
authors.forename: P T
authors.initials: PT
authors.lastname: Scheepers
authors.forename: D D
authors.initials: DD
authors.lastname: Velders
authors.forename: M M
authors.initials: MM
authors.lastname: Straetemans
authors.forename: J C
authors.initials: JC
authors.lastname: Ouwerkerk
authors.forename: L A
authors.initials: LA
authors.lastname: van Vliet
authors.forename: R P
authors.initials: RP
authors.lastname: Bos
mesh_terms: D000818:Animals; D003545:Cysteine / Q000032:analysis; D005260:Female; D005449:Fluorenes / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000032:analysis*; D006868:Hydrolysis; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents
chemical_list: D005449:Fluorenes; D006454:Hemoglobins; D007202:Indicators and Reagents; D012997:Solvents; C012177:2-aminofluorene; D003545:Cysteine