Electrophoretically defined myosin heavy chain patterns of single human muscle spindles.
At least four myosin heavy chain (MHC) isoforms were separated by SDS-PAGE in extracts of intrafusal fibers isolated by microdissection from human lumbrical muscles. The fastest migrating MHC represents a slow isoform. The slowest migrating MHC was identified as the embryonic MHCemb. A faint band, moving slightly faster than MHCemb, most likely represents a neonatal/fetal MHC isoform. A prominent band, moving between the latter and the slow isoform is suggested to represent a hitherto unidentified, spindle-specific MHC isoform, MHCif.
Pedrosa-Domellöf
F
F
Department of Anatomy, University of Umeå, Sweden.
Gohlsch
B
B
Thornell
L E
LE
Pette
D
D
D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D009470:Muscle Spindles / Q000737:chemistry* / Q000196:embryology; D009218:Myosins / Q000032:analysis*
D009218:Myosins