title: Multiple mismatch annealing: basis for random amplified polymorphic DNA fingerprinting.
abstract: In order to investigate the possible mechanism underlying the random amplified polymorphic DNA (RAPD) fingerprinting, we examined the origin and the nucleotide sequences of RAPD bands. Our data suggest that a number of sites in the genome are flanked by perfect or imperfect invert repeats, which permit multiple mismatch-annealing to occur between the single primer and the template DNA and eventually lead to the exponential amplification of the encompassing DNA segments.
authors.affiliation: Department of Immunology, University of Manitoba, Winnipeg, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Venugopal
authors.forename: S
authors.initials: S
authors.lastname: Mohapatra
authors.forename: D
authors.initials: D
authors.lastname: Salo
authors.forename: S
authors.initials: S
authors.lastname: Mohapatra
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D017931:DNA Primers; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D004247:DNA