title: Sex and regional differences in intracellular localization of estrogen receptor immunoreactivity in adult ferret forebrain.
abstract: Estrogen receptors were visualized in adult ferret brains using the H222 estrogen receptor antibody and immunocytochemical techniques. H222 immunoreactive (H222ir) cell nuclei were present in many forebrain regions in gonadectomized ferrets of both sexes. In many instances, H222ir cells also had immunoreaction product in their processes. All cells with H222ir processes also contained H222ir nuclei. More H222ir processes were observed in females in the medial and lateral preoptic area/anterior hypothalamus, and at the level of the descending fornix and caudal anterior commissure. Quantitative image analysis confirmed that females had significantly more (approximately 50%) extranuclear H222 immunoreaction product than males in cells in the magnocellular or preoptic subnuclei of the bed nucleus of the stria terminalis. Cells in the principal subnucleus of the bed nucleus of the stria terminalis and ventrolateral septum were notable for the relative paucity of H222ir processes. Sex differences in the intracellular extranuclear distribution of estrogen receptor protein in particular brain regions might contribute to the differential regulation of estrogen-dependent functions in the two sexes.
authors.affiliation: Department of Biochemistry and Developmental Neurobiology, Shriver Center, Waltham, Mass 02254.
authors.forename: S A
authors.initials: SA
authors.lastname: Tobet
authors.forename: T W
authors.initials: TW
authors.lastname: Chickering
authors.forename: T O
authors.initials: TO
authors.lastname: Fox
authors.forename: M J
authors.initials: MJ
authors.lastname: Baum
mesh_terms: D000818:Animals; D005260:Female; D005289:Ferrets; D007032:Hypothalamus, Anterior / Q000737:chemistry; D007150:Immunohistochemistry; D008297:Male; D009919:Orchiectomy; D010052:Ovariectomy; D011301:Preoptic Area / Q000737:chemistry; D016548:Prosencephalon / Q000737:chemistry*; D011960:Receptors, Estrogen / Q000032:analysis*; D012727:Sex Characteristics
chemical_list: D011960:Receptors, Estrogen