[Autoradiographic and biochemical studies of protein synthesis in nucleated erythrocytes during the formation of their segregation apparatus].
It has been shown that during novocaine (4.6 x 10(-3) M) induced formation of the segregation apparatus (SA) the total protein synthesis decreases, the synthesis of certain proteins being characterized by a high rate turnover. In the intact erythrocytes, 1 x 10(-2) cycloheximide (CHM) inhibits the novocaine induced formation of segregation apparatus (SA) and protein synthesis by 90%. The combined action of novocaine and CHM on erythrocytes is accompanied by a decrease in CHM inhibiting effect on protein synthesis. This effect retains for 1.5 h during the action of both compounds. Some vacuoles of SA disposed cytochemically exposed acid phosphatase (AP), which enabled us to consider these as lysosomes. According to AP distribution in lysosomes, they can be classified into 3 groups. In group 1 AP is distributed along the membrane of vacuoles, in group 2 it is associated with the material inside the lysosome, and in group 3 the enzyme is both distributed along the membrane and associated with the material enclosed within the segregation vacuole. Such a mode of AP distribution reflects presumably the functional heterogeneity of lysosomes.
Veselkina
M N
MN
Nilova
V K
VK
Bulychev
A G
AG
D000135:Acid Phosphatase / Q000097:blood / Q000187:drug effects; D000818:Animals; D001345:Autoradiography; D001798:Blood Proteins / Q000096:biosynthesis* / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D003864:Depression, Chemical; D004347:Drug Interactions; D004900:Erythroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008540:Meiosis / Q000187:drug effects; D011343:Procaine / Q000494:pharmacology; D011896:Rana temporaria; D014316:Tritium
D001798:Blood Proteins; D014316:Tritium; D011343:Procaine; D003513:Cycloheximide; D000135:Acid Phosphatase; D008239:Lysine
Avtoradiograficheskoe i biokhimicheskoe issledovanie sinteza belka v iadernykh éritrotsitakh v period formirovaniia v nikh segregatsionnogo apparata.