Characterization of novel fucosyl- and tyvelosyl-containing glycoconjugates from Trichinella spiralis muscle stage larvae.
The monosaccharide composition of an affinity-purified family of antigenically-related Trichinella spiralis larval glycoproteins was determined by gas chromatography/mass spectrometry. This group of 6 major glycoproteins, designated TSL-1, originates in the muscle stage (L1) larval stichosome. They are present on the L1 surface and in excretory/secretory products of L1 larvae, are stage-specific, and are highly immunodominant. The glycosyl composition of the TSL-1 antigens was remarkable in 2 respects: (1) fucose accounted for 36 molar percent of the glycosyl residues; and (2) a 3,6-dideoxyhexose was identified, which accounted for at least 24 molar percent of the glycosyl residues. Previously, 3,6-dideoxyhexoses have been found only in certain Gram-negative bacterial lipopolysaccharides and in ascaroside alcohols (ascarylose) of Ascaris eggs. The 3,6-dideoxyhexose found in the TSL-1 antigens also was found in ES. This Trichinella sugar has been chemically identified as a 3,6-dideoxyarabinohexose, the same as found in Ascaris eggs. However, the absolute configuration of the TSL-1 sugar is D-(tyvelose), not L-(ascarylose) as is found in Ascaris eggs. Methylation analysis indicated that the TSL-1 3,6-dideoxy-D-arabinohexose was present entirely as non-reducing terminal residues. Approximately 83% of the fucose was also present as non-reducing terminal residues, with the remaining fucose found as 3,4-linked branched residues.
Wisnewski
N
N
Department of Pathology, Colorado State University, Fort Collins.
McNeil
M
M
Grieve
R B
RB
Wassom
D L
DL
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006001:Glycoconjugates / Q000032:analysis*; D006601:Hexoses / Q000737:chemistry; D008745:Methylation; D017160:Trichinella spiralis / Q000737:chemistry* / Q000254:growth & development
D000947:Antigens, Helminth; D002241:Carbohydrates; D006001:Glycoconjugates; D006601:Hexoses