Sequence of a DNA probe specific for Anopheles quadrimaculatus species A (Diptera: Culicidae).
The nucleotide sequence was determined for a portion of a 12-kb genomic DNA clone specific for Anopheles quadrimaculatus species A. Four short, internally repeated sequences were identified. Synthetic oligonucleotide probes were prepared based on these four repeats. The oligonucleotides are highly specific and can be reliably used to separate individuals of An. quadrimaculatus species A from members of other species of the complex.
Johnson
D W
DW
Medical and Veterinary Entomology Research Laboratory, USDA-ARS, Gainesville, FL 32608.
Cockburn
A F
AF
Seawright
J A
JA
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015342:DNA Probes / Q000737:chemistry*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D015345:Oligonucleotide Probes