Modulation of functional activities of the neurotoxin from black widow spider venom.
We have studied the action of an alpha-latrotoxin (alpha-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC12 cells as well as on [14C]GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.
Grishin
E V
EV
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Himmelreich
N H
NH
Pluzhnikov
K A
KA
Pozdnyakova
N G
NG
Storchak
L G
LG
Volkova
T M
TM
Woll
P G
PG
D000595:Amino Acid Sequence; D000818:Animals; D001740:Black Widow Spider / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016716:PC12 Cells; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013111:Spider Venoms / Q000737:chemistry / Q000494:pharmacology*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D011506:Proteins; D013111:Spider Venoms; D005680:gamma-Aminobutyric Acid; C020763:alpha-latrotoxin; C038599:black widow spider venom