Immunoreactive fucosylceramide as a B-cell differentiation marker.
The reactivity of PC47H, a monoclonal antibody (mAb) directed against fucosylceramide, with cells of lymphoid lineage was examined. Immunoreactive fucosylceramide (FC) was recognized only in pokeweed mitogen (PWM)-stimulated B blasts, plasma cells and germinal center cells. mAb PC47H did not react with T cells at different stages or with peripheral blood B cells. Furthermore, FC was expressed abundantly in blastic cells of B-cell lymphoma, multiple lymphoma and myeloma cell lines KMS-12-BM and KMS-12-PE. In other words, FC was expressed more strongly in mature than in immature B cells. Immunoelectron microscopy showed that FC was located in the plasma membrane and rough endoplasmic reticulum. mAb PC47H can therefore be used as a unique B-cell differentiation marker for study of B-cell activation and differentiation and clonal analysis of lymphoid malignancies.
Nakamura
H
H
Department of Pathology, Saitama Medical Center, Saitama Medical School, Kawagoe, Japan.
Yamada
H
H
Kitagawa
H
H
Masunaga
A
A
Itoyama
S
S
Sugawara
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D015415:Biomarkers; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002478:Cells, Cultured; D002554:Cerebrosides / Q000276:immunology*; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D015415:Biomarkers; D002554:Cerebrosides; C070271:fucosylceramide