An in-house enzyme-linked immunoabsorbant assay for human growth hormone.
A simple, non-isotopic in-house enzyme-linked immunoabsorbant assay (ELISA) for human growth hormone (GH) was developed. The assay involved using in-house polyclonal anti-GH adsorbed onto 96-well microtitre plates, commercially prepared mouse monoclonal anti-GH, and goat anti-mouse IgG horseradish peroxidase detection system. Results of recovery and parallelism studies ranged from 95%-106% and 98%-101% respectively, of the expected values. The detection limit of the assay was 0.008 mIU/well or the equivalent to 0.4 mIU/L of undiluted serum. Intra- and interassay coefficients of variations were 4.8%-7.9% and 6.5%-8.7% respectively. Serum GH levels measured in this assay correlated well with those measured in established in-house radioimmunoassays (r = 0.985, p < 0.001) and immunoradiometric assay from NETRIA (r = 0.984, p < 0.001).
Nazaimoon
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Department of Biochemistry, Faculty of Medicine, National University of Malaysia, Kuala Lumpur.
Ng
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ML
Bak
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D000818:Animals; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D013006:Growth Hormone / Q000032:analysis*; D006801:Humans; D015592:Immunoradiometric Assay; D051379:Mice; D011817:Rabbits; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D013006:Growth Hormone