cAMP-induced changes of intracellular free Mg2+ levels in human erythrocytes.
To examine the role of cAMP in the regulation of intracellular free magnesium concentration ([Mg2+]i), we measured [Mg2+]i in human erythrocytes by 31P-NMR spectroscopy. (-)-Isoproterenol, forskolin, Bt2cAMP and 8-bromo-cAMP decreased [Mg2+]i in human erythrocytes. Bt2cAMP did not increase the efflux rate of Mg2+ from erythrocytes. HA1004, a potent inhibitor of cAMP-dependent kinases, markedly increased the [Mg2+]i in a Mg(2+)-free buffer solution. Addition of 8-bromo-cGMP or 12-O-tetradecanoylphorbol 13-acetate (TPA) did not affect the [Mg2+]i. These results suggest that beta-adrenergic stimulation and cAMP play an important role in the regulation of [Mg2+]i in human erythrocytes.
Matsuura
T
T
First Department of Internal Medicine, Osaka City University Medical School, Japan.
Kanayama
Y
Y
Inoue
T
T
Takeda
T
T
Morishima
I
I
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004163:Diphosphoglyceric Acids; D007546:Isoquinolines; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D019794:2,3-Diphosphoglycerate; D005576:Colforsin; D000255:Adenosine Triphosphate; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D000242:Cyclic AMP; D008274:Magnesium; D007545:Isoproterenol; D013755:Tetradecanoylphorbol Acetate