A mechanism of vitamin E-enhanced active oxygen formation in concanavalin A-stimulated alveolar macrophage.
Vitamin E had an enhancing effect on active oxygen generation in concanavalin A (Con A)-stimulated alveolar macrophages (AMs) of rats. An inhibitor against protein kinase C (PKC) 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) suppressed a large part of this vitamin E-related effect, but a calmodulin antagonist N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) did not inhibit the increase of active oxygen formation by vitamin E treatment. These results suggest that the activation of PKC presumably relates to the enhancing effect of vitamin E on active oxygen formation in Con A-stimulated AMs.
Yano
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T
Division of Applied Food Research, National Institute of Health & Nutrition, Tokyo, Japan.
Ichikawa
T
T
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000037:antagonists & inhibitors; D003208:Concanavalin A / Q000494:pharmacology*; D004075:Diglycerides / Q000378:metabolism; D004789:Enzyme Activation; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013449:Sulfonamides / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism
D002147:Calmodulin; D004075:Diglycerides; D007042:Hypoxanthines; D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D003208:Concanavalin A; D013481:Superoxides; D014810:Vitamin E; D019271:Hypoxanthine; C017967:W 7; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D014969:Xanthine Oxidase; D011493:Protein Kinase C; D010100:Oxygen; D002118:Calcium