Crystallization and preliminary X-ray studies of L-aspartase from Escherichia coli.
Single crystals of L-aspartate ammonia-lyase (L-aspartase) from Escherichia coli have been obtained by microdialysis at room temperature using polyethylene glycol 3350 and sodium acetate as co-precipitants. The crystals exhibit the symmetry of space group P2(1)2(1)2 with a = 156.5 A, b = 147.6 A, c = 102.5 A and diffract at least to 2.8 A.
Shi
W
W
Department of Chemistry, Pennsylvania State University, University Park 16802.
Kidd
R
R
Giorgianni
F
F
Schindler
J F
JF
Viola
R E
RE
Farber
G K
GK
D001220:Aspartate Ammonia-Lyase / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000648:ultrastructure; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology
D001426:Bacterial Proteins; D001220:Aspartate Ammonia-Lyase