The putative oncoprotein DEK, part of a chimera protein associated with acute myeloid leukaemia, is an autoantigen in juvenile rheumatoid arthritis.
The 45-kD autoantigen associated with juvenile rheumatoid arthritis (JRA) has been isolated from HeLa cell nuclei and purified about 2500-fold to near homogeneity in a five-step chromatographic procedure. Purification of the antigen was monitored by immunoblot assays using a nearly monospecific anti-45-kD serum from a child with JRA. Tryptic peptide mapping and partial amino acid sequencing of the purified 45-kD antigen demonstrated its identity with the DEK protein. DEK is a 43-kD protein of unknown function expressed by the putative oncogene dek located on chromosome 6. As a result of a (6;9) translocation offociated with a rare subtype of acute myeloid leukaemia a chimeric protein containing most of DEK amino acids at the N-terminus is found in leukaemic cells (von Linden et al., Mol Cell Biol. 1992; 12: 1687-97). The 43-kD DEK was detected by immunoblotting with serum from a patient with JRA in a variety of rat tissues, and was most abundant in the spleen and in bone marrow.
Sierakowska
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Department of Biochemistry, New York University School of Medicine 10016.
Williams
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Szer
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Szer
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D000595:Amino Acid Sequence; D000818:Animals; D001171:Arthritis, Juvenile / Q000276:immunology*; D001324:Autoantigens / Q000302:isolation & purification*; D002478:Cells, Cultured; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002868:Chromosomal Proteins, Non-Histone; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015513:Oncogene Proteins / Q000302:isolation & purification*; D010449:Peptide Mapping; D000075223:Poly-ADP-Ribose Binding Proteins; D051381:Rats
D001324:Autoantigens; D002868:Chromosomal Proteins, Non-Histone; C084738:DEK protein, human; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins