Participation of macrophages in chloramphenicol-potentiated carrageenin-induced peritonitis in rats.
1. We investigated the possible potentiating effect of chloramphenicol succinate (30 mg/kg, every 12 h for 4 days, ip) on the response of polymorphonuclear neutrophils to carrageenin (150 micrograms, ip) or dextran (100 micrograms, ip) in the peritoneal cavity of male Wistar rats (180-230 g; N = 12 in each group). 2. Chloramphenicol potentiated the cell migration induced by carrageenin (35%) but not that induced by dextran. Previous macrophage depletion in the peritoneal cavity by washing with sterile saline abolished the cell response, whereas a previous thioglycollate-induced increase in macrophage numbers enhanced the potentiating effect (60%). 3. These results suggest that the potentiating effect on polymorphonuclear neutrophil migration induced by chloramphenicol may be related to chemotactic factors released by macrophages.
Moraes
J R
JR
Departamento de Patologia Veterinária, Faculdade de Ciências Agárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.
Moraes
F R
FR
Bechara
G H
GH
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology*; D002701:Chloramphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology; D004357:Drug Synergism; D007958:Leukocyte Count / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010538:Peritonitis / Q000139:chemically induced*; D051381:Rats; D017208:Rats, Wistar
D002701:Chloramphenicol; D002351:Carrageenan; C026016:chloramphenicol succinate