title: Effects of syngeneic anti-IgE antibodies on the development of IgE memory and on the secondary IgE response.
abstract: The prolonged inhibition of IgE synthesis in mice caused by perinatal inoculation of IgE is attributable, at least in part, to the formation of anti-IgE antibodies. The induction of unresponsiveness with respect to IgE synthesis requires that IgE be administered during a brief interval (2 to approximately 10) days after birth, that corresponds with the time period during which anti-IgE antibodies are induced. Passive administration of syngeneic anti-IgE also inhibits IgE synthesis. We have now investigated the effect of anti-IgE on the induction of memory for IgE production and on secondary IgE responses. Syngeneic anti-IgE antibodies were found to inhibit secondary IgE responses directly during immunization or after adoptive transfer of primed cells. Anti-IgE did not, however, prevent the induction of memory cells for IgE synthesis or cause the loss of memory for IgE synthesis. Inhibition of a secondary IgE response was found to require the presence of anti-IgE and was lost when anti-IgE antibodies were cleared from the mouse. After the transfer of primed cells and secondary challenge anti-IgE was inhibitory only when given during the first 3 to 5 days, after which the primed cells became resistant to inhibition. The failure of anti-IgE to prevent the induction of IgE memory cells is discussed in terms of class switches that occur during the transition from IgM to IgE production.
authors.affiliation: Rosenstiel Research Center, Department of Biology, Brandeis University, Waltham, MA 02254.
authors.forename: S
authors.initials: S
authors.lastname: Haba
authors.forename: A
authors.initials: A
authors.lastname: Nisonoff
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000097:blood / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology*; D016219:Immunotherapy, Adoptive; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013997:Time Factors
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000941:Antigens; D006241:Haptens; C054401:trinitrophenyl keyhole limpet hemocyanin; D007073:Immunoglobulin E; D006433:Hemocyanins