Transmembrane neural cell-adhesion molecule (NCAM), but not glycosyl-phosphatidylinositol-anchored NCAM, down-regulates secretion of matrix metalloproteinases.
During embryogenesis interactions between cells and extracellular matrix play a central role in the modulation of cell motility, growth, and differentiation. Modulation of matrix structure is therefore crucial during development; extracellular matrix ligands, their receptors, extracellular proteinases, and proteinase inhibitors all participate in the construction, maintenance, and remodeling of extracellular matrix by cells. The neural cell-adhesion molecule (NCAM)-negative rat glioma cell line BT4Cn secretes substantial amounts of metalloproteinases, as compared with its NCAM-positive mother cell line BT4C. We have transfected the BT4Cn cell line with cDNAs encoding the human NCAM-B and -C isoforms. We report here that the expression of transmembrane NCAM-B, but not of glycosyl-phosphatidylinositol-linked NCAM-C, induces a down-regulation of 92-kDa gelatinase (matrix metalloproteinase 9) and interstitial collagenase (matrix metalloproteinase 1), indicating that cellular expression of the recognition molecule NCAM regulates the metabolism of the surrounding matrix.
Edvardsen
K
K
Panum Institute, University of Copenhagen, Denmark.
Chen
W
W
Rucklidge
G
G
Walsh
F S
FS
Obrink
B
B
Bock
E
E
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis / Q000502:physiology*; D002460:Cell Line; D017364:Collagenases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D004790:Enzyme Induction; D005109:Extracellular Matrix / Q000201:enzymology; D018093:Gelatinases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D005910:Glioma; D017261:Glycosylphosphatidylinositols / Q000502:physiology*; D006801:Humans; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D015816:Cell Adhesion Molecules, Neuronal; D017261:Glycosylphosphatidylinositols; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases