Placental lactogen-like proteins in the rabbit placenta.
Rabbit placentae and embryos at days 11 and 12 were analyzed by two-dimensional gel electrophoresis and by light microscopic histology for the presence of placental lactogen-like proteins. Immunoblotting and immunohistochemistry were performed by using a goat anti-human placental lactogen serum as well as a monoclonal mouse anti-human prolactin immunoglobulin; the results were similar. In the gel electrophoresis of placental tissue, three protein spots at pH 5.6 and 43, 39, and 35 kDa were immunostained; they were absent in the embryo. Immunoresponse was restricted to the cytotrophoblast. Immunofluorescent cells were mainly found on the proximal parts of the placental trabeculae.
Gründker
C
C
Philipps-Universität, Marburg, Germany.
Hrabé de Angelis
M
M
Kirchner
C
C
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D008970:Molecular Weight; D010449:Peptide Mapping; D010920:Placenta / Q000378:metabolism*; D010928:Placental Lactogen / Q000302:isolation & purification / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011817:Rabbits
D011257:Pregnancy Proteins; D010928:Placental Lactogen