Determination of acyclovir by ultrafiltration and high-performance liquid chromatography.
A simple, sensitive and rapid high-performance liquid chromatographic (HPLC) procedure to determine total serum acyclovir concentrations is described. The assay involves a heat inactivation step at 56 degrees C to prevent risk of infection, ultrafiltration as a pretreatment step prior to ion-pair reversed-phase liquid chromatography using guanosine as internal standard, and ultraviolet detection at 254 nm. This method has excellent recovery (97-100%), linearity (0.5-100 mg/l) and precision (1.2-8.0% coefficient of variation). The detection limit is 50 micrograms/l. The assay proved to be suitable for therapeutic drug monitoring of acyclovir.
Nebinger
P
P
Medizinisches Labor Dr. Enzenauer & Dr. Wilhelm, Osnabrück, Germany.
Koel
M
M
D000212:Acyclovir / Q000097:blood*; D001800:Blood Specimen Collection; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D014462:Ultrafiltration
D000212:Acyclovir