The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins.
Monospecific antibodies against an oligopeptide, conserved among the Mcm class of yeast replication proteins, were used to screen a human cDNA library. Eight of the isolated cDNA clones have the potential to code for sections of proteins with high sequence similarities to the yeast proteins Mcm3 and Cdc46 from Saccharomyces cerevisiae and Cdc21 from S. pombe. Our results establish a novel and highly conserved family of nuclear proteins in mammalian cells.
Hu
B
B
Division of Biology, Universitèt Konstanz, Germany.
Burkhart
R
R
Schulte
D
D
Musahl
C
C
Knippers
R
R
D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004261:DNA Replication; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D064126:Minichromosome Maintenance Complex Component 3; D064148:Minichromosome Maintenance Complex Component 4; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics*; D009842:Oligopeptides / Q000737:chemistry; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C066470:MCM3 protein, human; C489423:MCM5 protein, human; D009687:Nuclear Proteins; D009842:Oligopeptides; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D064126:Minichromosome Maintenance Complex Component 3; D064148:Minichromosome Maintenance Complex Component 4; C078490:mcm4 protein, S pombe