Cloning and characterization of the human t(3;6)(p14;p11) translocation breakpoint associated with hematologic malignancies.
The t(3;6)(p14;p11) chromosome translocation was identified in a family in which three members developed hematologic malignancies. To help characterize the region on chromosome 3 surrounding this translocation breakpoint, two flanking lambda clones, MS156 and MJ1525, were linked by pulsed-field gel electrophoresis to the same 510-kb NotI fragment on chromosome 3. MS156 was localized to a region proximal to the breakpoint of a der(3) chromosome somatic cell hybrid (derived from the t(3;6) cell line), and MJ1525 localized distal to the breakpoint. MJ1525 was used to screen the CEPH yeast artificial chromosome (YAC) library, which revealed a YAC, 195F3, that spanned the breakpoint. Subcloning into Lambda DASH II and production of a contiguous array of overlapping lambda clones revealed a clone, L17, that spanned the breakpoint. A rare restriction endonuclease map for the YAC 195F3 was constructed, and multiple clusters of rare restriction sites within the YAC were identified, possibly indicating the disruption of a gene by the t(3;6) translocation breakpoint.
Smith
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Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI 48201.
Joseph
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Nadeau
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Shridhar
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Gemmill
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Drabkin
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Knuutila
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Smith
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D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D018244:Chromosomes, Artificial, Yeast; D002893:Chromosomes, Human, Pair 3; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D006822:Hybrid Cells; D007938:Leukemia / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic