Efficacy of intravitreous interleukin-1 in the reabsorption of experimental vitreous hemorrhage in rabbits.
A low degree of phagocytosis in the vitreous is considered a major cause of the inadequate spontaneous removal of vitreous hemorrhage. We investigated the putative role of natural human interleukin-1 (IL-1) in the clearance of experimental vitreous hemorrhage and compared it with that of retinal cryotherapy. Five groups of 10 albino rabbits each were injected intravitreously with 0.1 ml autologous whole blood. Mean fundus revisualization times after blood clouding were as follows: group 1 controls (blood injection and no further manipulation), 6.1 weeks; group 2 (transconjunctival cryoapplication 7 days after blood injection), 4.3 weeks; group 3 (natural human IL-1 injected into the vitreous 7 days after blood injection), 3.8 weeks; group 4 (cryoapplication and IL-1 seven days after blood injection), 3.7 weeks; group 5 (intravitreous injection of phosphate-buffered saline), 4.1 weeks. Differences were statistically significant at week 4 of follow-up (p < 0.01). Two additional groups were used to estimate the possible toxicity of IL-1 (group 6) and PBS (group 7). Clinical and histologic examinations found no intraocular alterations imputable to IL-1. These results suggest that IL-1 may be used for nonsurgical treatment of human vitreous hemorrhage.
Pastor
J C
JC
Instituto de Oftalmobiologia Aplicada (IOBA), Valladolid Medical School, University of Valladolid, Spain.
Fernandez
C
C
D000042:Absorption; D000818:Animals; D002633:Chemotaxis; D003452:Cryosurgery; D004195:Disease Models, Animal; D007267:Injections; D007375:Interleukin-1 / Q000627:therapeutic use*; D011817:Rabbits; D014822:Vitreous Body; D014823:Vitreous Hemorrhage / Q000378:metabolism* / Q000601:surgery / Q000628:therapy
D007375:Interleukin-1