mRNA amplification system by viral replicase in transgenic plants.
We have constructed transgenic tobacco plants (M1x2-FCP2IFN plants) expressing viral RNA replication genes of brome mosaic virus (BMV) and BMV RNA3 derivative (FCP2IFN) carrying the human gamma interferon (IFN-gamma) gene. In M1x2-FCP2IFN plants the RNA3 derivative expressed from the integrated cDNA was replicated and subgenomic RNA (i.e. mRNA of IFN-gamma) was produced by BMV replicase. The accumulation level of the mRNA of IFN-gamma was approximately 5-fold higher than that by the cauliflower mosaic virus (CaMV) 35S RNA promoter. In addition IFN-gamma accumulated in M1x2-FCP2IFN plants.
Mori
M
M
Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Japan.
Kaido
M
M
Okuno
T
T
Furusawa
I
I
D018076:DNA, Complementary; D005784:Gene Amplification; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D009029:Mosaic Viruses / Q000201:enzymology*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana
D018076:DNA, Complementary; D012333:RNA, Messenger; D007371:Interferon-gamma; D012324:RNA-Dependent RNA Polymerase