[Eimeria tenella and Eimeria acervulina: an antigenic stimulation in vitro of cells from immune chickens induced by adoptive transfer of protection against avian coccidiosis].
The transfer of 5 x 10(7) or 10(8) spleen cells from E tenella-infected chickens to virgin animals after 12-20-h in vitro stimulation with whole sporozoite homogenates confers significant protection to recipients. The oocyst contents of ceca on d 7 post-infection with 20,000 E tenella oocysts were (1.33 +/- 1.10) x 10(6) in chickens which received 5 x 10(7) immune cells after 20-h in vitro stimulation and (4.64 +/- 2.85) x 10(6) in chickens receiving 5 x 10(7) stimulated cells from normal chickens (85% protection). Adoptive transfer by spleen cells revealed an asymmetric cross-protection between E tenella and E acervulina. Spleen cells from E tenella immune chickens protected only against a subsequent infection with the same parasite, while spleen cells from E acervulina immune chickens protected against infection with E acervulina (78%) but also against infection with E tenella (68% protection). The common antigen permits better stimulation, but common surface sporozoite antigens purified from E tenella sporozoites via anti-E acervulina biliary antibodies are capable of stimulating both types of cells without, however, changing their properties.
Rhalem
A
A
Institut agronomique et vétérinaire Hassan-II (IAV), Rabat-Instituts, Maroc.
Sahibi
H
H
Kazanji
M
M
Laurent
F
F
Berrag
B
B
Péry
P
P
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D003429:Cross Reactions; D004539:Eimeria / Q000276:immunology*; D016786:Eimeria tenella / Q000276:immunology; D016219:Immunotherapy, Adoptive / Q000662:veterinary*; D011201:Poultry Diseases / Q000517:prevention & control*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000953:Antigens, Protozoan
Eimeria tenella et Eimeria acervulina: une stimulation antigénique in vitro des cellules provenant de poulets immuns induit le transfert adoptif de la protection contre les coccidioses aviaires.