Quantitative two-site enzyme-linked immunosorbent assays for inhibin A, activin A and activin B.
We have developed three specific enzyme-linked immunosorbent assay (ELISA) formats which quantitate inhibin A in conditioned media and serum. The assays are sensitive in a range 0.078-5.0 ng/ml and have been characterized in terms of cross-reactivity to inhibin related proteins. The CK:CK assay format recognizes inhibin A, inhibin B and inhibin-related molecules, while the 9A9:CK assay format recognizes inhibin A and inhibin A precursors, but not free alpha-subunit. The 11B5:CK assay appears to recognize only mature 32 kDa inhibin A. Additionally, we have developed separate, specific ELISA formats which quantitate activin A and activin B. The assays have a range of 0.2-50 ng/ml and 0.4-50 ng/ml for activin A and recombinant activin B, respectively. These assays are presently being used to examine the concentration of inhibin A, activin A and activin B in clinical serum samples.
Woodruff
T
T
Department of Cell Culture and Fermentation, Research and Development, Genentech, Inc., South San Francisco, CA 94080.
Krummen
L
L
Baly
D
D
Garg
S
S
Allison
D
D
Sadick
M
M
Wong
W
W
Mather
J
J
Soules
M
M
D028341:Activins; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D038681:Follistatin; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007265:Inhibins / Q000032:analysis* / Q000097:blood; D009842:Oligopeptides; D010455:Peptides / Q000032:analysis* / Q000097:blood
D017077:Culture Media, Conditioned; D038681:Follistatin; D006023:Glycoproteins; D009842:Oligopeptides; D010455:Peptides; C044147:activin B; D028341:Activins; C048617:myelopeptides; D007265:Inhibins