title: Crystallization and preliminary X-ray analysis of human tissue factor extracellular domain.
abstract: The extracellular domain (residues 1 to 220) of human tissue factor has been cloned and expressed in Escherichia coli and purified to isoelectric homogeneity. Single crystals suitable for X-ray analysis have been obtained by vapour diffusion. They belong to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 with a = b = 45.2 A, c = 231.5 A, contain one molecule per asymmetric unit and diffract to 2.6 A resolution. Native and derivative data sets have been collected to 3.6 and 3.9 A, respectively.
authors.affiliation: Department of Biochemistry, Medical School, Edinburgh, Scotland.
authors.forename: C W
authors.initials: CW
authors.lastname: Boys
authors.forename: A
authors.initials: A
authors.lastname: Miller
authors.forename: K
authors.initials: K
authors.lastname: Harlos
authors.forename: D M
authors.initials: DM
authors.lastname: Martin
authors.forename: E G
authors.initials: EG
authors.lastname: Tuddenham
authors.forename: D P
authors.initials: DP
authors.lastname: O'Brien
mesh_terms: D018360:Crystallography, X-Ray; D005110:Extracellular Space / Q000737:chemistry; D006801:Humans; D011994:Recombinant Proteins; D013925:Thromboplastin / Q000648:ultrastructure*
chemical_list: D011994:Recombinant Proteins; D013925:Thromboplastin