A conserved 8 bp motif (GCYATCAY) in the 3'UTR of transition protein 2 as a putative target for a transcript stabilizing protein factor.
The 3'UTR of the transition protein 2 gene of several mammalian species was sequenced and the transcript level of this gene was determined by Northern blots. In all species tested so far, a Northern blot detectable transcript level was associated with the presence of a conserved 8 bp motif (GCYATCAY) 50 bp downstream of the stop codon. RNA-bandshift experiments indicate that this region is capable of binding a cytosolic protein factor from rat testis. These findings support our hypothesis that the low transcript level of the transition protein 2 gene in human is due to insufficient storage of the mRNA as ribonucleic/protein particle.
Schlüter
G
G
Institute for Human Genetics, University of Göttingen, Germany.
Schlicker
M
M
Engel
W
W
D000818:Animals; D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis / Q000235:genetics*; D003593:Cytoplasm / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D013728:Terminator Regions, Genetic / Q000235:genetics; D013737:Testis / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C053886:spermatid transition proteins