Metabolism of 5-aminoquinoline with liver cytosol enzyme.
In vitro oxidation and acetylation of 5-aminoquinoline by rabbit liver enzyme preparation has been investigated. Incubation of 5-aminoquinoline with cytosol fraction of the enzyme preparation in the presence of acetyl coenzyme-A gave rise to three different products, viz. 5-amino-2-hydroxy quinoline, 5-acetyl-aminoquinoline and 5-acetylamino-2-hydroxy quinoline due to the combined action of aldehyde oxidase and N-acetyl transferase. The metabolites thus obtained by enzyme catalysed reactions were isolated and separated by TLC, HPLC and subsequently characterised by IR and mass spectral fragmentation techniques.
Banoo
R
R
Department of Pharmacy, University of Dhaka, Bangladesh.
Matheison
D W
DW
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000634:Aminoquinolines / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003600:Cytosol / Q000201:enzymology; D005260:Female; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D011817:Rabbits
D000634:Aminoquinolines; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase; D000123:Acetyltransferases; C085129:5-aminoquinoline