title: [Cloning of beta-amylase gene from B. substitute and its expression in E. coli].
abstract: The gene coding for beta-amylase with raw starch-digestion ability was isolated from B. substitute R2 which was selected in this lab. The procedure used in this screening was developed in this work by the method named nutrition-restriction. The DNA fragment containing the beta-amylase gene was 5.25 kb in length. There was no difference in enzymological properties between the beta-amylase produced by the gene-donor strain and the expression product of E. coli. The yield of the cloned beta-amylase was over 500 IU/ml in our laboratory culture condition, the RDA value was 57%, and all of this enzyme were found to be secreted into medium.
authors.affiliation: Institute of Biophysics, Academic Sinica, Beijing.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Shen
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D001614:beta-Amylase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001614:beta-Amylase