[The detection of Pasteurella multocida toxin using a commercially available ELISA].
126 Pasteurella (P.) multocida strains isolated from nasal swabs of pigs were investigated for producing toxin, comparing tissue culture (EBL cells) and DAKO ELISA-PMT-Kit. With help of ELISA 13 toxigenic P. multocida strains were identified, with help of tissue culture 12 strains producing toxin. In 14 of 219 examined nasal swab bacteria cultures of pigs toxin of P. multocida was detected by the commercially available ELISA. Because of an abundant mixed bacteria culture in 7 of these 14 swabs suspected colonies of P. multocida could not be isolated.
Weber
A
A
Landesuntersuchungsamt für das Gesundheitswesen Nordbayern, Nürnberg.
Wachowitz
R
R
Singer
H
H
Heil-Franke
G
G
D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000032:analysis* / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D009297:Nasal Mucosa / Q000382:microbiology; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000302:isolation & purification* / Q000378:metabolism; D012222:Rhinitis, Atrophic / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C062258:Pasteurella multocida toxin
Zum Nachweis von Pasteurella multocida-Toxin mit Hilfe eines im Handel erhältlichen ELISA.