title: Characterization of a human chromosome 4 flow-sorted cosmid library.
abstract: A cosmid library has been constructed from a hamster x human hybrid cell line and gridded into 270 microtiter plates containing a total of 25,920 single colonies. Approximately 84% of the recombinants contain human material, with an average length of 29 kb. This library represents a nearly three-fold coverage of human chromosome 4. We investigated this library for presumptive genes, using a set of oligonucleotides detecting SpI and splice-site consensus sequences. The presence of simple repeat motifs was investigated in the cosmids using the oligonucleotides (GGATTT)3, (GGAT)4, (CAC)5, (GCC)5, (AGC)5, (GATA)4, (GACA)4, and (CA)8 as hybridization probes.
authors.affiliation: Department of Molecular Human Genetics, Ruhr-University, Bochum FRG.
authors.forename: O
authors.initials: O
authors.lastname: Riess
authors.forename: I
authors.initials: I
authors.lastname: Siedlaczck
authors.forename: S
authors.initials: S
authors.lastname: Kredtke
authors.forename: G
authors.initials: G
authors.lastname: Melmer
authors.forename: J T
authors.initials: JT
authors.lastname: Epplen
authors.forename: L L
authors.initials: LL
authors.lastname: Deaven
mesh_terms: D000818:Animals; D001483:Base Sequence; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003360:Cosmids; D006224:Cricetinae; D005434:Flow Cytometry; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012091:Repetitive Sequences, Nucleic Acid