A multiple primer pairs polymerase chain reaction for the detection of human genital papillomavirus types.
We describe a polymerase chain reaction (PCR) based on the simultaneous detection of multiple strains of papillomavirus in a single reaction tube. This PCR method was specific and sensitive. We have validated this multiplex procedure on a collection of typed cervical biopsies specimens, and applied it to the detection of viruses in some clinical samples.
Lucotte
G
G
Laboratoire d'Anthropologie Moléculaire, CEABH, U.E.R. de Cochin Port Royal, Paris, France.
François
M H
MH
Petit
M C
MC
Berriche
S
S
Réveilleau
S
S
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002584:Cervix Uteri / Q000382:microbiology; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis; D002577:Uterine Cervical Diseases / Q000175:diagnosis*; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis
D017931:DNA Primers; D004279:DNA, Viral