Regulation of renal cytochrome P450s by thyroid hormone in diabetic rats.
Effects of thyroid hormone treatment on renal P450 expression in the diabetic rats were investigated. Diabetes produced by streptozotocin induced CYP4A2 and P450 K-2 (similar form with CYP2C23) but not P450 K-4 (similar form with CYP4A8) and induced lauric acid hydroxylation activity. The serum thyroid hormone level was decreased with diabetes. Treatment of diabetic rats with thyroid hormone (T3) as well as with insulin reversed the increase in the levels of CYP4A2 and P450 K-2. Thyroidectomy also induced CYP4A2 and P450 K-2 in the rat kidney. The increase was reversed by treatment of thyroidectomized rats with T3. These findings suggest that expression of CYP4A2 and P450 K-2 in rat kidney is suppressively regulated by thyroid hormone and the decrease in thyroid hormone level in the diabetic state affects the levels of CYP4A2 and P450 K-2.
Imaoka
S
S
Laboratory of Chemistry, Osaka City University Medical School, Japan.
Nakamura
M
M
Ishizaki
T
T
Shimojo
N
N
Ohishi
N
N
Fujii
S
S
Funae
Y
Y
D000818:Animals; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004790:Enzyme Induction; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D013965:Thyroidectomy; D014284:Triiodothyronine / Q000494:pharmacology*
C000717351:Cyp2c23 protein, rat; D014284:Triiodothyronine; D013311:Streptozocin; D003577:Cytochrome P-450 Enzyme System; D000090762:Cytochrome P-450 CYP2J2