Plasmids and outer membrane proteins of Yersinia enterocolitica and related species of swine origin.
Yersinia enterocolitica and other pathogenic yersiniae harbor a plasmid termed pYV which is required for the full expression of virulence. The pYV codes for the release of a set of proteins called Yops and two outer membrane proteins Yad A and Ylp A. In the present study, 80 strains of Y. enterocolitica and related species were examined for the possession of the pYV and the ability to express Yops and Yad A. Only Y. enterocolitica belonging to serogroups O:1,2,3, O:3, O:5,27, O:8, and O:9 harbored the virulence plasmid and were positive for the presence of the ancillary proteins. The restriction fragment patterns of the pathogenic bioserotypes belonging to the same serogroup affiliation were similar irrespective of whether they were of swine origin, reference strains, or human clinical isolates. The same was also true of the electrophoretic patterns of the Yops. Our findings are in agreement with previous studies and support the view that pigs may be an important reservoir of pathogenic Y. enterocolitica.
Kwaga
J
J
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Iversen
J O
JO
D018829:Adhesins, Bacterial; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D015964:Gene Expression Regulation, Bacterial; D010957:Plasmids; D015183:Restriction Mapping; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence / Q000235:genetics; D015009:Yersinia Infections / Q000382:microbiology / Q000662:veterinary*; D015008:Yersinia enterocolitica / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; C064832:YadA protein, Yersinia