Calcium mobilization in isolated cochlear spiral ganglion cells of the guinea pig.
Single cochlear spiral ganglion cells (SGCs) were isolated using enzymatic and mechanical techniques. Intracellular free calcium ion concentrations ([Ca2+]i) in the SGCs were measured using a digital imaging microscope and the Ca(2+)-sensitive fluorescence dye fura-2. In the presence of the Ca2+ ionophore ionomycin (1 microM), there was an irreversible increase in [Ca2+]i. Depolarization by high K+ (150 mM) led to an increase in [Ca2+]i in SGCs, and this effect was reversible. The SGCs apparently possess voltage-gated calcium channels.
Han
D Y
DY
Department of Otolaryngology, Kansai Medical University Osaka, Japan.
Yamashita
T
T
Harada
N
N
Kumazawa
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D002478:Cells, Cultured; D016257:Fura-2; D006168:Guinea Pigs; D015759:Ionomycin / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D013136:Spiral Ganglion / Q000166:cytology / Q000378:metabolism*
D015220:Calcium Channels; D015759:Ionomycin; D011188:Potassium; D002118:Calcium; D016257:Fura-2