[Study on human gamma-crystallins: IV. Photooxidation of fetal gamma 2-crystallin by ultra-violet irradiation].
Human fetal gamma 2-crystallin solution was irradiated by ultra-violet (UV) (lambda 365 nm) at varying time, and analysed by light scattering, SDS-PAGE with DACM, which could specifically combine to free sulphydryl, as well as tryptophan fluorescence and its quenching with acrylamide. The results showed that light scattering of gamma 2-crystallin solution increased after 24 hour irradiation; SDS-PAGE reveled formation of dimer which did not have free sulphydryl and low molecular weight peptide of gamma 2-crystallin. The tryptophan fluorescence of gamma 2-crystallin decreased, and acrylamide fluorescence quenching effect did not show significant change. It suggested that human fetal gamma 2-crystallin photooxidized by UV could aggreate by disulfide and degrated to low molecular weight peptide, and its tryptophan were also oxidized in some degree.
Wu
K
K
National Ophthalmological Laboratories, Ministry of Public Health, Guangzhou, China.
Pan
S
S
Liang
S
S
Li
S
S
D003459:Crystallins / Q000528:radiation effects*; D005333:Fetus; D006801:Humans; D010084:Oxidation-Reduction; D012542:Scattering, Radiation; D014466:Ultraviolet Rays
D003459:Crystallins