The effect of nifedipine, a calcium channel blocker, on human spermatozoal functions.
In vitro addition of 0.1-100 microns of a calcium channel blocker, Nifedipine, resulted in a significant non-competitive inhibition in the uptake of Ca++. The activity of Ca(++)-dependent ATPase enzyme was also decreased. Motility of the spermatozoa was significantly arrested following incubation with different doses of the drug at 37 degrees C for different durations. The pattern of motility changed within two hours from rapid and linear progression to slow or sluggish linear or non-linear movement and finally to non-progressive motility or even immotility. Scanning electron microscopic studies revealed disruptive changes in the head as well as tail region and coiling of spermatozoa after Nifedipine treatment. An increase in the formation and release of malonyldialdehyde was observed following Nifedipine addition in a dose-dependent fashion. The membrane cholesterol and phospholipid contents were considerably lowered in the treated samples. The potential of such calcium channel blocking agent in the designing of male contraceptive programme is discussed.
Kanwar
U
U
Department of Zoology, Panjab University, Chandigarh, India.
Anand
R J
RJ
Sanyal
S N
SN
D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D009543:Nifedipine / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure
D010743:Phospholipids; D008315:Malondialdehyde; D002784:Cholesterol; D000252:Calcium-Transporting ATPases; D009543:Nifedipine; D002118:Calcium