Differences in plaque size and VP4 sequence found in SA11 virus clones having simian authentic VP4.
We isolated five stable SA11 clones (TN-S1, TN-S2, TN-L1, TN-L2, and BN-S4) with different plaque sizes from two SA11 stocks. In polyacrylamide gel electrophoresis, the mobilities of the fourth, fifth, and seventh RNA segments of SA11 clones with large plaque size (TN-L1 and TN-L2) were faster than those of clones with small plaque size (TN-S1, TN-S2, and BN-S4). Nucleotide sequence determination of the fourth RNA segment identified a five-amino-acid difference in VP4s between the clones with large and small plaque sizes. The VP4 sequences of two clones with small plaque size (TN-S1 and BN-S4) were the same as the sequence of SA11-SEM reported by K. Nishikawa et al. (1988, J. Virol. 62, 4022-4026), while the VP4 sequences of the clones with large plaque size (TN-L1 and TN-L2) were similar, but not identical, to that reported by D. B. Mitchell and G. W. Both (1989, Nucleic Acids Res., 17, 2122). A single-gene reassortant, K8-L2.4, in which RNA segment 4 was derived from clone TN-L2 of SA11 virus and the other RNA segments were from strain K8, produced large plaques like TN-L2, suggesting that the five-amino-acid difference in VP4 between the clones with large and small plaque sizes might be associated with the difference in plaque size.
Taniguchi
K
K
Department of Hygiene, School of Medicine, Sapporo Medical University, Japan.
Nishikawa
K
K
Kobayashi
N
N
Urasawa
T
T
Wu
H
H
Gorziglia
M
M
Urasawa
S
S
D000818:Animals; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D014644:Genetic Variation; D000882:Haplorhini; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D012401:Rotavirus / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D010948:Viral Plaque Assay
D036022:Capsid Proteins; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus