Cell-type specific expression of three rice genes GOS2, GOS5 and GOS9.
The cell-type-specific expression of three rice genes, GOS2, GOS5 and GOS9, was studied by mRNA in situ hybridization. Previous northern blot analysis revealed that these genes were constitutive, green tissue-specific and root-specific, respectively. In this study, GOS2 transcripts were observed in all leaf cell types. In roots, a temporal and spatial expression pattern was noticed. Higher mRNA levels were observed in lateral roots, especially in parenchymal cells of the vascular cylinder. Expression of GOS5 was mainly found in chloroplast-containing cells. For GOS9, significant levels of signal were observed in root and leaf sections.
Rey
P
P
Institute of Molecular Plant Sciences, Leiden University, The Netherlands.
Diaz
C
C
Schilperoort
R A
RA
Hensgens
L A
LA
D005786:Gene Expression Regulation; D017343:Genes, Plant; D017403:In Situ Hybridization; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014018:Tissue Distribution; D014158:Transcription, Genetic
C081177:GOS2 protein, Oryza sativa; C084806:GOS9 protein, Oryza sativa; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C070731:photosystem I, psaH subunit