Quantitative enzyme cytochemistry during human macrophage development.
Integrating microdensitometry was used to study changes in the intracellular activity of 4 enzymes during macrophage development. Suspension cultures of blood monocytes from 19 healthy human subjects were examined at 0, 2, 4 and 6 d. Mononuclear phagocytes were harvested by glass adherence and standard methods were used for cytochemical staining for NADH dehydrogenase, succinate dehydrogenase, acid phosphatase and alpha-naphthyl butyrate esterase. All specimens from all subjects were stained at the same time and staining intensities in individual cells were measured at appropriate wavelengths. A highly significant increase in enzyme activity with culture time was found for all 4 enzymes. These increases in mitochondrial, lysosomal and ectoenzyme activities during development indicate the increasing functional capabilities of the macrophages.
Sokol
R J
RJ
Department of Medicine, University of Sheffield, UK.
Hudson
G
G
Wales
J M
JM
Goldstein
D J
DJ
James
N T
NT
D000135:Acid Phosphatase / Q000097:blood; D000328:Adult; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology; D009245:NADH Dehydrogenase / Q000097:blood; D013385:Succinate Dehydrogenase / Q000097:blood; D013997:Time Factors
D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase; D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase; D000135:Acid Phosphatase