Distribution of protein kinase C in the villi of duodenum of developing chick embryos and chicks: immunohistochemical studies at the light and electron microscopic levels.
We examined the immunohistochemical localization of protein kinase C (PKC) in chick embryonic and chick duodenal villi contained in cultured duodena using monoclonal antibodies that recognize types 1, 2 and 3 at the light and electron microscopic levels. In the light microscopic studies, staining with the type 2 and 3 PKC antibodies revealed immunoreactivities in the absorptive epithelial cells of the villi that approximated the time course after hatching, but was weak and partial just before the time of hatching. The epithelial cells of crypts seemed to lose their stain at all times. In the transmission electron microscopic studies, positive immunoreaction was found predominantly in the endoplasmic reticulum and nucleus, and also in the terminal web in the absorptive epithelial cells of villi. In the cultured duodenal fragments, a change in the distribution of PKC was observed on the sheet-like duodenal fragments and its immunoreaction was mainly seen on the basal side of absorptive cells. In contrast, no change in the staining of PKC was revealed on the ring-like duodenal fragments. These results suggested that PKC in the chick duodenal villi may be functionally related with the differentiation of absorptive epithelial cells and sensitive to changes in the cellular interactions of absorptive epithelial cells.
Noda
S
S
Department of Morphology, School of Medicine, Tokai University, Isehara, Japan.
Inoue
T
T
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004386:Duodenum / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D004848:Epithelium / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D011493:Protein Kinase C / Q000032:analysis*
D011493:Protein Kinase C