title: Organization of mini-exon and 5S rRNA genes in the kinetoplastid Trypanoplasma borreli.
abstract: Mini-exon gene repeats from Trypanoplasma borreli, which belongs to the Cryptobiidae family of the Bodonina suborder of the Kinetoplastida, were isolated by PCR amplification and cloning. The presence of kinetoplastid-like mini-exon genes in T. borreli is consistent with the taxonomic status of this organism as a kinetoplastid protozoan. Two families of repeats were found: 597 nt (T1) and 794 nt (T2), each of which encodes an approximately 95-nt medRNA transcript. The T1 repeats also contain a complete 5S rRNA gene on the complementary strand. The T2 repeats contain a defective copy of a 5S gene, in which the 5' portion is absent. The intergenic regions between the 5'-ends of the mini-exon genes and the 5S rRNA genes in the T1 and T2 repeats are highly diverged. All or most mini-exon genes and 5S genes are located within either the T1 or the T2 repeats. The T1 repeats were localized to a megabase-size chromosome, while the T2 repeats were localized within at least 4 large chromosomes.
authors.affiliation: Department of Biology, University of California, Los Angeles 90024-1662.
authors.forename: D A
authors.initials: DA
authors.lastname: Maslov
authors.forename: M G
authors.initials: MG
authors.lastname: Elgort
authors.forename: S
authors.initials: S
authors.lastname: Wong
authors.forename: H
authors.initials: H
authors.lastname: Pecková
authors.forename: J
authors.initials: J
authors.lastname: Lom
authors.forename: L
authors.initials: L
authors.lastname: Simpson
authors.forename: D A
authors.initials: DA
authors.lastname: Campbell
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D015139:Blotting, Southern; D016054:DNA, Protozoan; D005091:Exons; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D005810:Multigene Family; D016053:RNA, Protozoan / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014345:Trypanosoma / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012341:RNA, Ribosomal, 5S