title: Polymorphism in BoLA-DRB3 exon 2 correlates with resistance to persistent lymphocytosis caused by bovine leukemia virus.
abstract: The relationship between polymorphism of the bovine lymphocyte Ag (BoLA)-DRB3 gene and resistance and susceptibility to persistent lymphocytosis (PL) caused by bovine leukemia virus (BLV) was investigated. Exon 2 of the BoLA-DRB3 gene was cloned from animals with BoLA haplotypes previously found to be associated with resistance and susceptibility to PL. Sequence analysis revealed the presence of the amino acids Glu-Arg (ER) at putative Ag binding residues 70 and 71 only in BoLA haplotypes associated with resistance to PL. This correlation was confirmed in a case control study (n = 26) using an allele-specific polymerase chain reaction for the detection of ER at residues 70-71. These results provide a molecular basis for Ir gene control of resistance and susceptibility to PL and suggest that the cellular immune response is important in preventing the in vivo spread of BLV infection.
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign 61801.
authors.forename: A
authors.initials: A
authors.lastname: Xu
authors.forename: M J
authors.initials: MJ
authors.lastname: van Eijk
authors.forename: C
authors.initials: C
authors.lastname: Park
authors.forename: H A
authors.initials: HA
authors.lastname: Lewin
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D016583:Enzootic Bovine Leukosis / Q000209:etiology* / Q000235:genetics / Q000276:immunology; D005091:Exons; D006239:Haplotypes; D001909:Leukemia Virus, Bovine / Q000472:pathogenicity*; D008285:Major Histocompatibility Complex; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017386:Sequence Homology, Amino Acid
chemical_list: D004247:DNA