Fluorescent dye (DiI) reveals the sensory cells in the lingual epithelium: a confocal laser scanning microscopic study.
Fluorescent carbocyanine dye was applied to the glossopharyngeal nerve of axolotls fixed with paraformaldehyde. Three cell types in the lingual epithelium were transneuronally labeled: chemosensory taste receptor cells and presumably mechanosensory basal cells in the taste buds, and epithelial cells with unknown function in the non-taste lingual epithelium.
Nagai
T
T
Department of Physiology, Teikyo University School of Medicine, Tokyo, Japan.
D000558:Ambystoma mexicanum; D000818:Animals; D002232:Carbocyanines; D004847:Epithelial Cells; D005456:Fluorescent Dyes; D005930:Glossopharyngeal Nerve / Q000648:ultrastructure; D006651:Histocytochemistry; D007834:Lasers; D008856:Microscopy, Fluorescence; D009475:Neurons, Afferent / Q000648:ultrastructure*; D013650:Taste Buds / Q000502:physiology / Q000648:ultrastructure; D014059:Tongue / Q000166:cytology* / Q000294:innervation
D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine