title: 31P NMR spectra of intact red blood cells: quantitation of UDPGlucose and UDPGalactose.
abstract: The combined levels of uridine diphosphogalactose (UDPGal) and uridine diphosphoglucose (UDPGlu) were measured directly by 31P NMR spectroscopy in intact fully oxygenated erythrocytes. Quantitative analysis was obtained using a sealed capillary of methylene diphosphonate (MDP) calibrated with standard solutions of UDPGlu and UDPGal of known concentration prepared in KRB/BSA. The combined peaks of UDPGlu and UDPGal were integrated after subtraction of the underlying broad component originating from membrane phospholipids. The average concentration of 27.16 +/- 5.18 nmole/ml or 8.08 +/- 1.36 mumole/100 g hemoglobin obtained for these metabolites correlated well with their total determined by HPLC of trichloracetic acid (TCA) extracts of the same samples.
authors.affiliation: Department of Pediatrics, University of Pennsylvania School of Medicine.
authors.forename: S L
authors.initials: SL
authors.lastname: Wehrli
authors.forename: M J
authors.initials: MJ
authors.lastname: Palmieri
authors.forename: R A
authors.initials: RA
authors.lastname: Reynolds
authors.forename: S
authors.initials: S
authors.lastname: Segal
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D014531:Uridine Diphosphate Galactose / Q000378:metabolism*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism*
chemical_list: D014531:Uridine Diphosphate Galactose; D014532:Uridine Diphosphate Glucose