title: Co-culture with granulosa cells does not increase the fertilization rate in couples with previous fertilization failures.
abstract: Ten couples included in our in-vitro fertilization programme, selected because of one of more previous total fertilization failures or a low fertilization rate (< 20%), were entered in an experimental protocol of egg insemination on autologous granulosa cells. Half the oocytes from each patient were randomly assigned to either a control or a co-culture group. We observed no difference in the fertilization rate between the control (16.2%) and co-culture groups (12.1%). Only two couples benefited from this technique since fertilization was obtained only in co-culture. The poor efficiency of this protocol led us to propose the use of sperm micro-injection if all classical attempts to improve fertilization were unsuccessful.
authors.affiliation: U173 INSERM, Hôpital Necker, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Plachot
authors.forename: J
authors.initials: J
authors.lastname: Mandelbaum
authors.forename: A M
authors.initials: AM
authors.lastname: Junca
authors.forename: J M
authors.initials: JM
authors.lastname: Antoine
authors.forename: J
authors.initials: J
authors.lastname: Salat-Baroux
authors.forename: J
authors.initials: J
authors.lastname: Cohen
mesh_terms: D002478:Cells, Cultured; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000166:cytology*; D006801:Humans; D009865:Oocytes / Q000166:cytology*; D017211:Treatment Failure