A procedure for the purification of thioredoxin-m from leaves of the C4 plant Zea mays.
A simple four-step procedure for the purification of thioredoxin-m from Zea mays leaves is described. The procedure provides pure protein with recoveries of 20-25%. This thioredoxin mediates in the regulation of NADP-malate dehydrogenase involved in photosynthesis in C4 plants.
Agostino
A
A
Division of Plant Industry, CSIRO, Canberra, Australia.
Hatch
M D
MD
D011232:Chemical Precipitation; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D010940:Plant Proteins / Q000302:isolation & purification*; D013879:Thioredoxins / Q000302:isolation & purification*; D003313:Zea mays / Q000737:chemistry*
D010940:Plant Proteins; D013879:Thioredoxins