Osteoconductive properties of methylpyrrolidinone chitosan in an animal model.
Bone defects were surgically produced in the tibiae of rabbits and medicated with freeze-dried methylpyrrolidinone chitosan. Histological observations 60 d after surgery showed a considerable presence of neoformed bone tissue, as opposed to controls, originating from the pre-existing bone as well as from the periosteum. The cationic nature and the chelating ability of the methylpyrrolidinone chitosan apparently favoured mineralization. Endosteal-periosteal and bone marrow osteoblast-like precursors, stimulated by growth factors entrapped in the coagulum-polysaccharide mixture, gave rise to intramembranous bone formation. The ultrastructural examination evidenced that bone osteoid was followed by mineralization of the tissue.
Muzzarelli
R A
RA
Institute of Biochemistry, Faculty of Medicine, University of Ancona, Italy.
Zucchini
C
C
Ilari
P
P
Pugnaloni
A
A
Mattioli Belmonte
M
M
Biagini
G
G
Castaldini
C
C
D000818:Animals; D001844:Bone Conduction / Q000187:drug effects*; D001846:Bone Development / Q000187:drug effects; D001847:Bone Diseases / Q000188:drug therapy; D001861:Bone Regeneration / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D048271:Chitosan; D004195:Disease Models, Animal; D005260:Female; D008027:Light; D008853:Microscopy; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000187:drug effects*; D011760:Pyrrolidinones / Q000494:pharmacology*; D011817:Rabbits; D013977:Tibia / Q000601:surgery
D011760:Pyrrolidinones; C085060:methylpyrrolidinone chitosan; D002686:Chitin; D048271:Chitosan