title: Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line.
abstract: Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 10(8.5), 10(3.5), and 10(2.5) tissue culture infective dose 50 (TCID50)/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome virus isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.
authors.affiliation: U.S. Meat Animal Research Center, USDA, Clay Center, Nebraska.
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: J
authors.initials: J
authors.lastname: Kwang
authors.forename: I J
authors.initials: IJ
authors.lastname: Yoon
authors.forename: H S
authors.initials: HS
authors.lastname: Joo
authors.forename: M L
authors.initials: ML
authors.lastname: Frey
mesh_terms: D000818:Animals; D018018:Arterivirus / Q000502:physiology*; D002460:Cell Line; D002999:Clone Cells; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D012328:RNA Viruses / Q000502:physiology*; D013552:Swine; D014779:Virus Replication