title: Detection of trisomy 12 on ovarian sex cord stromal tumors by fluorescence in situ hybridization.
abstract: Trisomy of chromosome 12 has been frequently described in various neoplasms, particularly in tumors of the female genitourinary tract. Fluorescence in situ hybridization with a centromeric repetitive DNA probe, specific for chromosome 12, was done to detect such cytogenic changes on frozen-tissue sections from 10 cases of ovarian sex cord stromal tumors. The case series was composed by granulosa cell tumors (four cases), fibromas (four cases), thecoma (one case), and Sertoli-Leydig cell tumor (one case). In granulosa cell tumors, the range of trisomy was 12 to 32% and in fibromas 8 to 22%, whereas in the single case of thecoma trisomy was present in 8% and in the Sertoli-Leydig cell tumor in 4% of the nuclei examined. These results represent an additional series of cases of trisomy 12 in ovarian neoplasms, namely, in ovarian sex cord stromal tumors.
authors.affiliation: Department of Genetics, Yale University, New Haven, Connecticut.
authors.forename: D
authors.initials: D
authors.lastname: Taruscio
authors.forename: M L
authors.initials: ML
authors.lastname: Carcangiu
authors.forename: D C
authors.initials: DC
authors.lastname: Ward
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002881:Chromosomes, Human, Pair 12; D005260:Female; D005350:Fibroma / Q000235:genetics / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D018309:Neoplasms, Gonadal Tissue / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D018312:Sex Cord-Gonadal Stromal Tumors / Q000235:genetics*; D014314:Trisomy / Q000235:genetics*