HPLC-fluorescence determination of bile acids in pharmaceuticals and bile after derivatization with 2-bromoacetyl-6-methoxynaphthalene.
2-Bromoacetyl-6-methoxynaphthalene was used as a pre-chromatographic fluorescent labelling reagent for the high-performance liquid chromatographic (HPLC) analysis of bile acids. The derivatization reaction was performed in an aqueous medium in the presence of tetrahexylammonium bromide by ultrasonication at 40 degrees C to give fluorescent esters which were separated by reversed-phase HPLC and detected fluorimetrically (lambda ex = 300 nm, lambda em = 460 nm). Applications to the determination of ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) in their pharmaceutical formulations are described. The method was also applied to the determination of free and conjugated bile acids in human bile samples.
Cavrini
V
V
Department of Pharmaceutical Sciences, University of Bologna, Italy.
Gatti
R
R
Roda
A
A
Cerrè
C
C
Roveri
P
P
D001646:Bile / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000032:analysis*; D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009281:Naphthalenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014580:Ursodeoxycholic Acid / Q000031:analogs & derivatives / Q000032:analysis*
D001647:Bile Acids and Salts; D009281:Naphthalenes; D002635:Chenodeoxycholic Acid; C084840:2-bromoacetyl-6-methoxynaphthalene; D014580:Ursodeoxycholic Acid