Detection of tumor suppressor gene and oncogene in So-Rb50 human retinoblastoma cell line.
Retinoblastoma (Rb) is the most common malignant cancer of eye. So-Rb50 is the first Rb cell line established in China in 1988. It has passed to the 387th passage now. We collected cells of the 327th passage of SO-Rb50, purified its genomic DNA and detected it with Rb and c-myc cDNA probes respectively (normal human white blood cells DNA was the control). We found the Rb gene was deleted while c-myc gene was amplified three times. This provides a basis for further study of the regulation of tumor development and tumor reversal with this cell line in vitro.
Wang
X
X
Department of Biochemistry, Beijing Medical University, China.
Zeng
F
F
Xu
Z
Z
Zheng
Y
Y
Wang
L
L
D005134:Eye Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005784:Gene Amplification; D016161:Genes, Retinoblastoma; D016147:Genes, Tumor Suppressor; D016259:Genes, myc; D006801:Humans; D007223:Infant; D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured