High resolution microfluorometry of Ca2+ signalling in dendrites and spines of central neurons.
Using an upright microscope and an interface type slice chamber for epifluorometric imaging of fura-2 injected neurons in brain slice is particularly convenient to obtain good spatial resolution. Using this approach we found that muscarinic activation uncouples K-channel activation from intradendritic Ca and that postsynaptic spines represent independent compartments for Ca-activated processes when activated synaptically.
Müller
W
W
Roche Institute of Molecular Biology, Department of Neurosciences, Roche Research Center, Nutley, NJ 07110.
Connor
J A
JA
D000818:Animals; D002118:Calcium / Q000502:physiology*; D003712:Dendrites / Q000502:physiology*; D005470:Fluorometry; D016257:Fura-2; D006168:Guinea Pigs; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D066298:In Vitro Techniques; D009474:Neurons / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology; D017966:Pyramidal Cells / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013569:Synapses / Q000378:metabolism / Q000502:physiology
D015221:Potassium Channels; D011976:Receptors, Muscarinic; D002118:Calcium; D016257:Fura-2