Circadian rhythm of neuropeptide Y-like immunoreactivity in the iris-ciliary body of the rat.
Neuropeptide Y (NPY)-like immunoreactivity (LI) in the iris-ciliary body of rats kept under constant darkness (DD) and in 12 h light-12 h dark cycle (LD) was determined by enzyme immunoassay. NPY-LI contents in the iris-ciliary body were found to oscillate in circadian fashion under DD and LD conditions, with a peak at about circadian time 12 (CT 12) and a trough at around CT 0. Unilateral superior cervical ganglionectomy caused a significant decrease in NPY-LI levels in the sympathectomized eye compare to the contralateral intact eye, independent of lighting phase. These results suggest the presence of an endogenous circadian rhythm in NPY-LI content in the rat iris-ciliary body, and the possible involvement of a sympathetic input from the superior cervical ganglion.
Otori
Y
Y
Laboratory of Integrative Brain Function, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Cagampang
F A
FA
Yamazaki
S
S
Inouye
S T
ST
Mano
T
T
Tano
Y
Y
D000818:Animals; D002924:Ciliary Body / Q000294:innervation / Q000378:metabolism*; D002940:Circadian Rhythm; D003623:Dark Adaptation; D005728:Ganglia, Sympathetic / Q000601:surgery; D015171:Ganglionectomy; D007124:Immunoenzyme Techniques; D007498:Iris / Q000294:innervation / Q000378:metabolism*; D008027:Light; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009478:Neuropeptide Y