title: Production of colony-stimulating factor 1 by T cells: possible involvement in their interaction with antigen-presenting cells.
abstract: Colony-stimulating factor 1 (CSF-1) is required for the growth and differentiation of mononuclear phagocytes, and is also involved in modulating various activities in mature cells. We report herein that T-cell lines produce 4.6 and 1.5 kb mRNA species of CSF-1, and express the CSF-1 protein on their outer membranes, as determined by immunofluorescence staining with anti-CSF-1 antibodies. The CSF-1 protein is biologically active. Interested by the possible immunoregulatory function of CSF-1, we assessed its effect in an assay of antigen presentation to the T cell lines. We found that anti-CSF-1 antibodies inhibited T-cell stimulation. Moreover, soluble CSF-1 could not overcome this inhibition, but exerted a significant inhibitory activity on the interaction between T cells and antigen-presenting cells leading to T-cell activation and proliferation in vitro. Based on these observations we propose that T-cell CSF-1 may be involved in the interaction of these cells with CSF-1 receptor bearing antigen-presenting cells.
authors.affiliation: Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Zisman
authors.forename: A
authors.initials: A
authors.lastname: Waisman
authors.forename: E
authors.initials: E
authors.lastname: Ben-Yair
authors.forename: B
authors.initials: B
authors.lastname: Tartakovsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D015152:Blotting, Northern; D002450:Cell Communication; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D007739:L Cells; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000941:Antigens; D010455:Peptides; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor