Recombinant secreted haemagglutinin protects mice against a lethal challenge of influenza virus.
Balb/c mice were immunized with 2 x 2 micrograms of purified recombinant secreted haemagglutinin, derived from the A/Victoria/3/75 (H3N2) virus. In the first immunization, Ribi adjuvant was used, while for the booster injection a monophosphoryl lipid A/muramyl dipeptide combination was chosen. Mice immunized in this way were 90-100% protected against a challenge with 20 LD50 of mouse-adapted, homologous virus (strain X47). Bromelain-solubilized haemagglutinin gave only 70% protection under comparable conditions.
Vanlandschoot
P
P
Laboratory of Molecular Biology, Gent University, Belgium.
Maertens
G
G
Jou
W M
WM
Fiers
W
W
D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000502:physiology / Q000627:therapeutic use*; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000627:therapeutic use*
D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic