Influence of growth factors on in vitro invasiveness and type IV collagenolysis of human renal cell carcinoma cells.
We investigated the influence of epidermal growth factor (EGF) and transforming growth factor (TGF)-beta 1 on the in vitro invasion and type IV collagenolytic activity of two new cell lines of renal cell carcinoma (SRCC-1P and SRCC-1M). When cells were treated with EGF or with TGF-beta 1, EGF increased the number of cells penetrating through the reconstituted basement membrane in SRCC-1P. In contrast, TGF-beta 1 suppressed the number of cells penetrating through the membrane in SRCC-1M. In accordance with the invasiveness, EGF enhanced the activity of type IV collagenolysis in SRCC-1P, and TGF-beta 1 suppressed it in SRCC-1M. The growth factors did not affect DNA synthesis of the cells as evaluated by 3H-thymidine incorporation. These results suggest that EGF and TGF-beta 1 can influence the in vitro invasive process of renal cell carcinoma cells through their actions on proteolysis such as type IV collagenolysis.
Otani
N
N
Department of Urology, School of Medicine, Sapporo Medical University, Japan.
Tsukamoto
T
T
Masumori
N
N
Saiki
I
I
Yoneda
J
J
Kumamoto
Y
Y
D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology*; D003094:Collagen / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000502:physiology*; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D016212:Transforming Growth Factor beta / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D003094:Collagen