title: Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair.
abstract: Yeast RNA polymerase II initiation factor b, homolog of human TFIIH, is a protein kinase capable of phosphorylating the C-terminal repeat domain of the polymerase; it possesses a DNA-dependent ATPase activity as well. The 85 kd and 50 kd subunits of factor b are now identified as RAD3 and SSL1 proteins, respectively; both are known to be involved in DNA repair. Factor b interacts specifically with another DNA repair protein, SSL2. The ATPase activity of factor b may be due entirely to that associated with a helicase function of RAD3. Factor b transcriptional activity was unaffected, however, by amino acid substitution at a conserved residue in the RAD3 nucleotide-binding domain, suggesting that the ATPase/helicase function is not required for transcription. These results identify factor b as a core repairosome, which may be responsible for the preferential repair of actively transcribed genes in eukaryotes.
authors.affiliation: Department of Cell Biology, Stanford University School of Medicine, California 94305.
authors.forename: W J
authors.initials: WJ
authors.lastname: Feaver
authors.forename: J Q
authors.initials: JQ
authors.lastname: Svejstrup
authors.forename: L
authors.initials: L
authors.lastname: Bardwell
authors.forename: A J
authors.initials: AJ
authors.lastname: Bardwell
authors.forename: S
authors.initials: S
authors.lastname: Buratowski
authors.forename: K D
authors.initials: KD
authors.lastname: Gulyas
authors.forename: T F
authors.initials: TF
authors.lastname: Donahue
authors.forename: E C
authors.initials: EC
authors.lastname: Friedberg
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D000251:Adenosine Triphosphatases / Q000502:physiology*; D001483:Base Sequence; D004265:DNA Helicases / Q000502:physiology*; D017931:DNA Primers / Q000737:chemistry; D004260:DNA Repair; D005656:Fungal Proteins / Q000502:physiology*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000737:chemistry / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; C083908:SSL1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D051758:Transcription Factor TFIIH; D000251:Adenosine Triphosphatases; C061897:Rad3 protein, S cerevisiae; D004265:DNA Helicases