Laser-induced fluorescence detection of 9-fluorenylmethyl chloroformate derivatized amino acids in capillary electrophoresis.
Laser-induced fluorescence (LIF) was applied to the detection of 9-fluorenylmethyl chloroformate (FMOC-Cl) derivatized amino acids separated by capillary electrophoresis. Fluorescence excitation was provided by a pulsed, KrF laser operating at 248 nm. A limit of detection of 5 x 10(-10) M was obtained for FMOC-alanine (S/N = 2). Separation of FMOC-derivatized proline, hydroxyproline, and sarcosine was achieved with a 20 mM borate buffer (pH 9.2), and the separation of FMOC-derivatized amino acid standard mixture was obtained using a 20 mM borate buffer (pH 9.2) containing 25 mM sodium dodecyl sulfate.
Chan
K C
KC
Program Resources, Inc./DynCorp, NCI-Frederick Cancer Research and Development Center, MD 21702.
Janini
G M
GM
Muschik
G M
GM
Issaq
H J
HJ
D000596:Amino Acids / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D005449:Fluorenes / Q000032:analysis*; D007202:Indicators and Reagents; D007834:Lasers; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000596:Amino Acids; D005449:Fluorenes; D007202:Indicators and Reagents; C054007:1-(9-fluorenyl)methyl chloroformate