title: Serotonin-2 receptors in the CNS of alcohol-preferring and -nonpreferring rats.
abstract: The densities of serotonin-2 (5-HT2) receptors, labelled with [3H]ketanserin (Ket), were examined in the CNS of alcohol-naive, adult, male alcohol-preferring (P) and -nonpreferring (NP) rats using quantitative autoradiography. The densities of binding sites labelled with 2.0 nM [3H]Ket were 15-25% lower (p < 0.05) in layer IV of the medial prefrontal, frontal, cingulate, parietal, and temporal cortices of the P line compared with the NP line. [3H]Ket binding in the P rats was also 40-50% lower (p < 0.05) in the medial and lateral nucleus accumbens, olfactory tubercle, and caudate-putamen, and 20% lower (p < 0.05) in the claustrum, compared with the NP line. No differences in 2.0 nM [3H]Ket binding were observed between the lines in the piriform cortex, hypothalamus, amygdala, hippocampus, ventral tegmental area, substantia nigra, central gray, and interpenduncular nucleus. Scatchard analysis of [3H]Ket binding (0.5-0.6 nM, determined in layer IV of the frontal and parietal cortex and in the lateral nucleus accumbens, indicated lower Bmax values without a change in Kd for the P compared to the NP rats. The results of this study indicate that there are fewer 5-HT2 receptors in certain CNS regions of the P relative to the NP rats.
authors.affiliation: Department of Psychiatry, Indiana University School of Medicine, Indianapolis.
authors.forename: W J
authors.initials: WJ
authors.lastname: McBride
authors.forename: E
authors.initials: E
authors.lastname: Chernet
authors.forename: J A
authors.initials: JA
authors.lastname: Rabold
authors.forename: L
authors.initials: L
authors.lastname: Lumeng
authors.forename: T K
authors.initials: TK
authors.lastname: Li
mesh_terms: D000428:Alcohol Drinking / Q000523:psychology*; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D007650:Ketanserin / Q000378:metabolism / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D012702:Serotonin Antagonists
chemical_list: D012702:Serotonin Antagonists; D007650:Ketanserin