title: Low temperature dependent IgG3 subclass antibody with low avidity to C100-3 antigen derived from hepatitis C virus in voluntary blood donors.
abstract: The presence of an antibody which reacts strongly at lower temperatures to C100-3 antigen derived from the hepatitis C virus (HCV) was demonstrated in blood donors' sera. These sera reacted with the antigen most strongly at 4 C and most weakly at 37 C and are termed 'cold antibody group'. The cold antibody group reaction was specifically inhibited with soluble C100-3 antigen. Cold antibody bound to C100-3 antigen at 4 C was dissociated at 37 C and was isolated in this manner. Low temperature-dependent anti C100-3 antibody was shown to be predominantly IgG3 subclass by ELISA using anti-human IgG subclass antibody. This IgG3 antibody was dissociated from antigen with 6M urea, demonstrating its low avidity. HCV-RNA was demonstrated in two of five samples. Therefore, to achieve more effective prevention of post-transfusion hepatitis C, we propose the incorporation of programs to detect low temperature-dependent low-avidity antibody against HCV-related antigen.
authors.affiliation: Japanese Red Cross Central Blood Center, Tokyo.
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: C
authors.initials: C
authors.lastname: Matsumoto
authors.forename: J
authors.initials: J
authors.lastname: Watanabe
authors.forename: N
authors.initials: N
authors.lastname: Yahagi
authors.forename: K
authors.initials: K
authors.lastname: Nishioka
mesh_terms: D000915:Antibody Affinity; D000956:Antigens, Viral; D001782:Blood Donors; D015153:Blotting, Western; D003450:Cryoglobulins; D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis / Q000453:epidemiology; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification*; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral; D011994:Recombinant Proteins; D013696:Temperature; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; C064544:C100 protein, hepatitis C virus; D003450:Cryoglobulins; D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D012367:RNA, Viral; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins