Location of cAMP-dependent protein kinase type I with the TCR-CD3 complex.
Selective activation of cyclic adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase type I (cAKI), but not type II, is sufficient to mediate inhibition of T cell replication induced through the antigen-specific T cell receptor-CD3 (TCR-CD3) complex. Immunocytochemistry and immunoprecipitation studies of the molecular mechanism by which cAKI inhibits TCR-CD3-dependent T cell replication demonstrated that regulatory subunit I alpha, along with its associated kinase activity, translocated to and interacted with the TCR-CD3 complex during T cell activation and capping. Regulatory subunit II alpha did not. When stimulated by cAMP, the cAKI localized to the TCR-CD3 complex may release kinase activity that, through phosphorylation, might uncouple the TCR-CD3 complex from intracellular signaling systems.
Skålhegg
B S
BS
Institute of Medical Biochemistry, University of Oslo, Blindern, Norway.
Taskén
K
K
Hansson
V
V
Huitfeldt
H S
HS
Jahnsen
T
T
Lea
T
T
D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000032:analysis / Q000378:metabolism*; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D006801:Humans; D007152:Immunologic Capping; D047908:Intracellular Signaling Peptides and Proteins; D008213:Lymphocyte Activation; D010766:Phosphorylation; D011233:Precipitin Tests; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000032:analysis / Q000378:metabolism*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D017260:Receptor-CD3 Complex, Antigen, T-Cell; C015482:protein kinase modulator; D000242:Cyclic AMP; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases