[The effect of 1-isoproterenol on monovalent cation transport in activated human lymphocytes].
Potassium (rubidium) influx, sodium and potassium contents, as well as protein contents and 3H-thymidine incorporation have been investigated in quiescent lymphocytes, PHA-stimulated lymphocytes and T-lymphocytes, activated by human recombinant interleukin 2 (IL-2) within 0.5, 24 and 48 h. In quiescent lymphocytes ouabain-sensitive Rb+ influx was diminished by I-isoproterenol and forskolin. Both the cAMP elevating agents decreased the enhancement of ouabain-sensitive and ouabain-resistant Rb+ influxes in PHA-stimulated lymphocytes in 24 and 48 h. The IL-2 activation of T-cells during 24 and 48 h was accompanied by the elevation of Rb+ influxes and Ki content. L-isoproterenol inhibited ouabain-sensitive Rb+ influx in activated T-cells. This inhibition was found to correlate with 3H-thymidine incorporation in cells. Thus, the effects of l-isoproterenol and forskolin on ionic transport in human activated lymphocytes correlated with a well-known cAMP inhibition of lymphocyte proliferation.
Krasnikova
T L
TL
Marakhova
I I
II
Vinogradova
T A
TA
D001693:Biological Transport, Active / Q000187:drug effects; D002414:Cations, Monovalent / Q000097:blood; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000097:blood; D012413:Rubidium / Q000097:blood; D012964:Sodium / Q000097:blood; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D002414:Cations, Monovalent; D005576:Colforsin; D010042:Ouabain; D012964:Sodium; D007545:Isoproterenol; D012413:Rubidium; D011188:Potassium
Vliianie 1-izoproterenola na transport monovalentnykh kationov v aktivirovannykh limfotsitakh cheloveka.