title: Novel oligosaccharides with the sialyl-Le(a) structure in human milk.
abstract: Two novel oligosaccharides with the sialyl-Le(a) structure were isolated from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography, and structurally characterized by a combination of 600-MHz 1H NMR spectroscopy and fast atom bombardment mass spectrometry. The structural studies indicated the structures of these oligosaccharides to be: [formula: see text]
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: H
authors.initials: H
authors.lastname: Nakada
authors.forename: S
authors.initials: S
authors.lastname: Fukui
authors.forename: I
authors.initials: I
authors.lastname: Funakoshi
authors.forename: T
authors.initials: T
authors.lastname: Kawasaki
authors.forename: I
authors.initials: I
authors.lastname: Yamashina
authors.forename: S
authors.initials: S
authors.lastname: Tate
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
mesh_terms: D000911:Antibodies, Monoclonal; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D000911:Antibodies, Monoclonal; D009844:Oligosaccharides; C058981:sialyl-Le(a) oligosaccharide