Intracellular pH regulation in cecal epithelial cells from the chick.
Intracellular pH (pHi) regulation has been investigated in cells isolated from the proximal ceca of the chicken. pHi was measured with the pH-sensitive dye, 2',7'-bis(carboxyethyl)-5 (6)-carboxyfluorescein in nominally HCO(3-)-free solutions. Under resting conditions the pHi was 7.08. Removal of extracellular Na+ decreased pHi by approx. 0.24 pH units and the subsequent addition of Na+ increased pHi towards the control value. This Na(+)-dependent pHi recovery was inhibited by 5-(N-ethyl-N-isopropyl)amiloride (EIPA). Following an intracellular acidification, by abrupt withdrawal of NH4Cl, pHi alkalinized in the nominally absence of Na+. Rotenone, N-ethylmaleimide, N,N'-dicyclohexylcarbodiimide, 4-chloro-7-nitrobenz-2-oxa-1,3-diazole, iodoacetic acid and SCH 28080 inhibited the Na(+)-independent pHi recovery rate by 82, 82, 67, 74, 77 and 50% respectively. Bafilomycin A1 was without effect. Na(+)-independent cell alkalization was stimulated by external K+. In the presence of N-ethylmaleimide addition of Na+ induced a rapid pHi recovery. The initial rate of this recovery exhibited first-order dependence on Na+ concentration and it was inhibited by EIPA. The initial rate of Na(+)-dependent cell alkalization increased with a Hill coefficient greater than one when pHi was reduced from 7.2 to 6.2. The 'set point' for the exchanger is approx. 7.5. These studies demonstrate that in cecal epithelial cells exist at least two mechanisms for proton secretion: a Na(+)-H+ exchanger and a Na(+)-independent proton transport system.
Calonge
M L
ML
Departamento de Fisiología y Biología Animal, Facultad de Farmacia, Universidad de Sevilla, Spain.
Peral
M J
MJ
Ilundáin
A
A
D009327:4-Chloro-7-nitrobenzofurazan / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D002432:Cecum / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004072:Digitonin / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D007700:Kinetics; D009550:Nigericin / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014634:Valinomycin / Q000494:pharmacology
D000897:Anti-Ulcer Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007093:Imidazoles; D007461:Iodoacetates; C035235:Sch 28080; D012402:Rotenone; D014634:Valinomycin; D004024:Dicyclohexylcarbodiimide; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D012964:Sodium; D009327:4-Chloro-7-nitrobenzofurazan; D004072:Digitonin; D005033:Ethylmaleimide; D009550:Nigericin; D019807:Iodoacetic Acid