[Screening of L-glutamate oxidase forming strains and conditions for enzyme production].
A strain P-26 producing extracellular L-glutamate oxidase was screened out from 125 Actinomyces. The formation of enzyme was induced by L-glutamate, it reached maximum when the microorganism was grown in wheat bran medium containing 0.2% L-glutamate, 0.5% Na2CO3 and 100% H2O at 28 degrees C for 6 days. The enzyme activity could be moderately enhanced by extra supplement of inorganic salts such as MgSO4 x 7H2O and CaCl2.
Xu
S
S
Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai.
Li
Y
Y
D000190:Actinomyces / Q000201:enzymology / Q000302:isolation & purification*; D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D012988:Soil Microbiology
D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase