[Synergism in the stimulating effect of transforming growth factor beta and insulin on the substrate-independent proliferation of the CHO-719 cell line].
Cells of line CHO-719 from the Chinese hamster ovary have no epidermal growth factor receptors. It was detected that these cells were significantly stimulated to proliferate in a semisolid culture medium containing 0.33% agar by transforming growth factor beta (TGF-beta) in combination with insulin. This effect was even more pronounced when the cells of line CHO-719, adapted to the growth in the presence of low concentration (3%) of fetal bovine serum, were used. TGF-beta and insulin utilized separately exerted no influence on the cellular growth.
Sushel'nitskiĭ
S I
SI
Iakimovich
I A
IA
Stoĭka
R S
RS
Kusen'
S I
SI
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D010053:Ovary / Q000166:cytology / Q000187:drug effects*; D013268:Stimulation, Chemical; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D007328:Insulin; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor
Sinergizm stimuliruiushchego vliianiia transformiruiushchego faktora rosta beta i insulina na substratnezavisimuiu proliferatsiiu kletok linii CHO-719.