Plausible involvement of a diabetic serum factor in neutrophil membrane pathology.
With a view to determining the role of diabetic serum factor (DSF) in the progression of membrane pathology, time-dependent preincubation effects of DSF on certain membrane-bound enzymes of normal polymorphonuclear leucocytes (PMNL) have been studied. DSF is found to cause significant decrements in the activities of Na+/K+ ATPase, PLC and AcE of PMNL on in vitro incubation for 60 min, while the effect on Ca2+/Mg2+ ATPase appears only on prolonged incubation with an initial hike in activity at 5 min. The implications of these results have been discussed.
Nath
N
N
Department of Biochemistry, Nagpur University.
Biswas
S
S
Bhelwa
A
A
Ittoop
A
A
Biswal
S
S
D000110:Acetylcholinesterase / Q000097:blood; D000328:Adult; D001685:Biological Factors / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood; D010738:Type C Phospholipases / Q000097:blood
D001685:Biological Factors; D000110:Acetylcholinesterase; D010738:Type C Phospholipases; D000254:Sodium-Potassium-Exchanging ATPase