Marrow adipocytes regulate growth and differentiation of osteoblasts.
Conditioned media (CM) from various marrow subpopulations (MBA series) were examined for their effects on growth and maturation of marrow-derived osteoblastic cells, MBA-15. CM harvested from a stromal adipocytes, 14F1.1, stimulated cell growth, collagen but not non-collagenous proteins synthesized by MBA-15 cells. Alkaline phosphatase activity was inhibited under the same culture conditions. These results were associated with morphological changes, i.e., the large cuboidal MBA-15 cells acquired a fibroblast-like appearance. Exposure of MBA-15 cells to known growth factors: BMP-2, TGF beta, IGF-I and PDGF or combined with 14F1.1 CM resulted in a dominant effect of the latter. This may imply that marrow adipocytes produce factor/s that participate in the stromal regulation of osteoblastic functions.
Benayahu
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Department of Histology and Cell Biology, Sackler School of Medicine, Tel-Aviv University, Israel.
Zipori
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Wientroub
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D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D001853:Bone Marrow / Q000502:physiology*; D019485:Bone Morphogenetic Proteins; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis; D017077:Culture Media, Conditioned; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D014176:Protein Biosynthesis; D011506:Proteins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D019485:Bone Morphogenetic Proteins; D017077:Culture Media, Conditioned; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D011506:Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D000469:Alkaline Phosphatase