Limited diffusibility of gene products directed by a single nucleus in the cytoplasm of multinucleated myofibres.
Two types of beta-galactosidase genes, whose products are distributed in the nucleus (N beta-gal) or cytoplasm (C beta-gal), were injected with fructose intramuscularly into the quadriceps of adult mice. Regionally restricted and overlapped distributions of both gene products were observed in the myofibres. These findings indicate that N beta-gal is incorporated into the nucleus responsible for its synthesis and that C beta-gal becomes located in the vicinity of the nucleus after its synthesis. This restricted location of C beta-gal in myofibres remained unchanged during the development of infant mouse muscle. Thus, the gene products directed by the nucleus of myofibres seem to show limited diffusibility, suggesting a universal localization of subcellular domains in myofibres.
Ono
T
T
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Ono
K
K
Mizukawa
K
K
Ohta
T
T
Tsuchiya
T
T
Tsuda
M
M
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D003593:Cytoplasm / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004058:Diffusion; D016189:Dystrophin / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D007700:Kinetics; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure*; D009136:Muscular Dystrophies / Q000628:therapy; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D016189:Dystrophin; D004247:DNA; D008156:Luciferases; D001616:beta-Galactosidase