DNA segments mapped by reciprocal use of microsatellite primers between mouse and rat.
Rat microsatellite primers were used for detection of homologous DNA segments in the mouse species (Mus laboratorius, Mus musculus musculus, and Mus spretus). Twenty five (16.3%) of 153 rat primer pairs amplified specific DNA segments, when genomic DNA of mice was used as a template in the polymerase chain reaction (PCR). Size variation among inbred strains of mice was found for 13 DNA segments (8.5%). Eight out of the 13 polymorphic DNA segments were mapped to a particular chromosome with two sets of recombinant inbred strains, AKXL or BXD. Similarly, mouse microsatellite primers were used for detection of homologous DNA segments in rats (Rattus norvegicus). Twenty (12.0%) of 166 primer pairs amplified specific DNA segments from rat genome. Size variation among inbred strains of rats was found for seven DNA segments (4.2%). Eleven of these 20 DNA segments were mapped with a rat x mouse somatic cell hybrid clone panel and/or linkage analysis by use of backcross progeny. Our results suggest that the mapped DNA segments are really homologs between mouse and rat. These polymorphic DNA segments are useful genetic markers.
Kondo
Y
Y
Institute of Laboratory Animals, Faculty of Medicine, Kyoto University, Japan.
Mori
M
M
Kuramoto
T
T
Yamada
J
J
Beckmann
J S
JS
Simon-Chazottes
D
D
Montagutelli
X
X
Guénet
J L
JL
Serikawa
T
T
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D017931:DNA Primers; D004276:DNA, Satellite; D005260:Female; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers