title: Solubilisation partial characterisation of the alpha-MSH receptor on primary rat Schwann cells.
abstract: The ACTH/MSH melanocortin core peptide sequence possesses neurotrophic properties in peripheral nerve. During functional neuroanatomical recovery after damage to peripheral nerves, Schwann cells play a significant role in facilitating regeneration. Here we employ a modified super-potent alpha-MSH analogue to solubilise alpha-MSH receptor proteins from cultured primary rat Schwann cells. [125I-Tyr2,Nle4,D-Phe7,ATB-Lys11]-alpha-MSH photoaffinity labelled proteins from Schwann cells were analyzed by SDS-PAGE followed by autoradiography. The results indicate that the alpha-MSH receptor proteins labelled have a molecular weight of 42-45 kDa. These data are the first to demonstrate solubilisation and characterisation of alpha-MSH receptors from non-melanoma cells.
authors.affiliation: Department of Zoology, School of Biological Sciences, University of Bristol, UK.
authors.forename: J K
authors.initials: JK
authors.lastname: Dyer
authors.forename: A R
authors.initials: AR
authors.lastname: Ahmed
authors.forename: G W
authors.initials: GW
authors.lastname: Oliver
authors.forename: C W
authors.initials: CW
authors.lastname: Poulton
authors.forename: L W
authors.initials: LW
authors.lastname: Haynes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D011979:Receptors, Pituitary Hormone / Q000378:metabolism*; D012583:Schwann Cells / Q000378:metabolism*; D012995:Solubility; D000521:alpha-MSH / Q000378:metabolism
chemical_list: D011979:Receptors, Pituitary Hormone; D000521:alpha-MSH; C029446:MSH receptor