Screening retroviral packaging cells for highly efficient virus production by using a combined selection procedure.
To facilitate the screening for clones of transfected packaging cells producing a high yield of recombinant retrovirus, we present a fast and simple method for the isolation of overexpressing cells. By this method the efficiency of virus production can generally be enhanced 10- to 100-fold by application of high selection pressure. Cell lines which exhibit titers of up to 10(8) CFU/ml were obtained.
Wirth
M
M
GBF-Gesellschaft für Biotechnologische Forschung mbH, Genetics of Eukaryotes, Braunschweig, Germany.
Grannemann
R
R
Klehr
D
D
Hauser
H
H
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers; D009052:Leukemia Virus, Murine / Q000235:genetics; D051379:Mice; D009355:Neomycin / Q000494:pharmacology; D011533:Proviruses / Q000235:genetics; D011691:Puromycin / Q000494:pharmacology; D012190:Retroviridae / Q000235:genetics* / Q000254:growth & development*; D012641:Selection, Genetic; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014776:Virus Cultivation; D014779:Virus Replication
D005819:Genetic Markers; D011691:Puromycin; D013762:Tetrahydrofolate Dehydrogenase; D009355:Neomycin