Production of threonine by Brevibacterium flavum containing threonine biosynthesis genes from Escherichia coli.
Genes of the threonine operon of Escherichia coli were used for the construction of a Brevibacterium flavum strain excreting threonine. Using the shuttle vector pCEM300 and a newly constructed shuttle vector pEC71 (7.1 kb, Kmr/Nmr), various plasmids carrying E. coli thr genes were prepared. Mutants resistant to the threonine analog 2-amino-3-hydroxyvaleric acid (AHV) were isolated after the ethyl methanesulfonate treatment of B. flavum carrying these recombinant plasmids. A mutant of B. flavum CCM 351 carrying the cloned genes thrA and thrB accumulated 12 g/L of threonine after 48 h of cultivation.
Pátek
M
M
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
Hochmannová
J
J
Nesvera
J
J
D001951:Brevibacterium / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors; D006715:Homoserine Dehydrogenase / Q000378:metabolism; D008239:Lysine / Q000096:biosynthesis; D009876:Operon; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D010957:Plasmids; D013912:Threonine / Q000096:biosynthesis / Q000235:genetics*
D013912:Threonine; D006715:Homoserine Dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor); C021370:homoserine kinase; D008239:Lysine