Rapid miniprep isolation of mitochondrial DNA from metacestodes, and free-living and parasitic nematodes.
A method, based on one to isolate supercoiled plasmid DNA from bacterial cells, has been developed to purify mitochondrial DNA (mtDNA) from cestode and nematode tissue easily and efficiently. Starting with as little as 100 mg of helminth tissue, sufficient mtDNA for electrophoretic analysis was extracted. This DNA was essentially free of nuclear DNA and readily digested by restriction endonucleases. Approximately 20% of the mtDNA in helminth tissue was recovered, which is a significant improvement over previously available techniques.
Peloquin
J J
JJ
Department of Nematology, University of California, Riverside 92521.
Bird
D M
DM
Platzer
E G
EG
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D002498:Centrifugation; D002589:Cestoda / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D003720:Densitometry; D005260:Female; D008632:Mermithoidea / Q000235:genetics; D051379:Mice; D009348:Nematoda / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D013621:Taenia / Q000235:genetics
D004272:DNA, Mitochondrial