title: Use of lambda gt11 and monoclonal antibodies to map the gene for the 60,000 dalton glycoprotein of infectious laryngotracheitis virus.
abstract: To localize the gene encoding the 60 kD glycoprotein (gp60) of infectious laryngotracheitis virus (ILTV), a library of the ILTV genome was constructed in the lambda gt11 expression vector. Twelve recombinant bacteriophages expressing gp60 epitopes as fusion products with beta-galactosidase were detected by immunoscreening with monoclonal antibodies specific for gp60. The ILTV DNA sequence contained in one of these recombinants lambda 24-4 was used as a hybridization probe for mapping the insert sequence on the viral genome. The gene for the gp60 was located at map unit 0.72-0.77 in the unique long region (UL) of the ILTV genome. The DNA sequence of the 1.2 kb insert of lambda 24-4 containing the gp60 epitope was determined. The majority of deduced gp60 amino acid sequence has no homology with any of the known alphaherpesvirus glycoproteins.
authors.affiliation: CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Kongsuwan
authors.forename: M A
authors.initials: MA
authors.lastname: Johnson
authors.forename: C T
authors.initials: CT
authors.lastname: Prideaux
authors.forename: M
authors.initials: M
authors.lastname: Sheppard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002645:Chickens; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary; D001352:Herpesvirus 1, Gallid / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000517:prevention & control; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006023:Glycoproteins; D014764:Viral Proteins