Simultaneous determination of ethylene glycol, propylene glycol, 1,3-butylene glycol and 2,3-butylene glycol in human serum and urine by wide-bore column gas chromatography.
A method has been developed for the separation and measurement of ethylene glycol and three other glycols (propylene glycol, 1,3-butylene glycol and 2,3-butylene glycol) in biological samples by wide-bore column gas chromatography with a flame ionization detector. The method used 1,3-propylene glycol (1,3-propanediol) as an internal standard. The method was linear at least from 2 to 1000 micrograms/ml, with a detection limit of 1 microgram/ml. Analytical recoveries were 89-98% for the different concentrations. Precision studies showed coefficients of variation of 1.5-7.7% for the different concentrations. The assay was applied to the analysis of biological samples from two patients who had ingested ethylene glycol and/or other glycols in a suicide attempt.
Houzé
P
P
Département de Biochimie-Pharmacologie-Toxicologie, C.H.R. de Versailles, Le Chesnay, France.
Chaussard
J
J
Harry
P
P
Pays
M
M
D000328:Adult; D001897:Boronic Acids; D002849:Chromatography, Gas; D006018:Glycols / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D008297:Male; D011489:Protein Denaturation; D013406:Suicide, Attempted
D001897:Boronic Acids; D006018:Glycols; C010686:benzeneboronic acid