[Basic study of CA125 measurement using a newly developed "SD-8729" IRMA kit].
"SD-8729" is a one-step IRMA kit employing OC125 antibody as 125I-labeled tracer and M-11 antibody as an immunoadsorbent. Higher bound-radioactivity to beads was observed with shorter incubation time than that of a currently used CA125 IRMA kit which employed OC125 antibody both as 125I-labeled ligand and immunoadsorbent attached to beads. Almost identical CA125 values were obtained by using two kits. The antigenic nature recognized by the M-11 antibody seems substantially different from those of 130-22 or 145-9 antibodies recognizing CA130 antigen.
Yamaguchi
A
A
Department of Nuclear Medicine, School of Medicine, Gunma University.
Kobayashi
M
M
Imai
T
T
Otake
H
H
Matsubara
K
K
Hatori
N
N
Tomiyoshi
K
K
Inoue
T
T
Endo
K
K
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods*; D010051:Ovarian Neoplasms / Q000175:diagnosis; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D011933:Reagent Kits, Diagnostic