title: Metabolic response to standardised exercise test in standardbred trotters with red cell hypervolaemia.
abstract: Plasma concentrations of lactate, amino acids, ammonia and products of purine catabolism were studied before, during and after a standardised incremental exercise test in 29 Standardbred trotters admitted to the clinic for exercise tolerance testing. According to their red cell volume the horses were divided into red cell normovolaemic and red cell hypervolaemic (polycythaemic) groups. The exercise-response curve for taurine differed significantly in the two groups, whereas all the other amino acids behaved similarly. The [branched-chain amino acid]/[alanine] ratio, a proposed indicator for the use of amino acids in gluconeogenesis, was at rest significantly higher in the polycythaemic horses. Post-exercise concentrations of ammonia and allantoin, both end products of ATP breakdown, were lower in the polycythaemic horses. No differences were observed in the VLA4 and V200 markers for lactate and heart rate responses to incremental exercise, the oxidative capacity of the gluteus medius muscle, the enzyme activities or the post-exercise concentration of lactate, uric acid and hypoxanthine. It is concluded that horses with red cell hypervolaemia behave in a submaximal standardised exercise test on a treadmill in the same way as do red cell normovolaemic horses. The results suggest that the rate of amino acid utilisation in gluconeogenesis and the ability of amino acids to produce energy aerobically may be elevated in polycythaemic horses.
authors.affiliation: Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: A R
authors.initials: AR
authors.lastname: Pösö
authors.forename: B
authors.initials: B
authors.lastname: Essén-Gustavsson
authors.forename: S G
authors.initials: SG
authors.lastname: Persson
mesh_terms: D000481:Allantoin / Q000097:blood; D000596:Amino Acids / Q000097:blood; D000641:Ammonia / Q000097:blood; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D001806:Blood Urea Nitrogen; D004909:Erythrocyte Indices; D005080:Exercise Test / Q000662:veterinary; D005260:Female; D006339:Heart Rate; D006734:Horse Diseases / Q000209:etiology / Q000378:metabolism*; D006736:Horses; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000097:blood; D007773:Lactates / Q000097:blood; D008297:Male; D015999:Multivariate Analysis; D009132:Muscles / Q000201:enzymology / Q000473:pathology; D010805:Physical Conditioning, Animal / Q000009:adverse effects*; D005082:Physical Exertion / Q000502:physiology*; D011086:Polycythemia / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*; D014527:Uric Acid / Q000097:blood
chemical_list: D000596:Amino Acids; D001798:Blood Proteins; D007042:Hypoxanthines; D007773:Lactates; D014527:Uric Acid; D019271:Hypoxanthine; D000481:Allantoin; D000641:Ammonia