In vitro degradation of very low density lipoprotein from diabetic patients by lipoprotein lipase.
Fatty acid release by incubation with lipoprotein lipase (LPL) in vitro from very low density lipoproteins (VLDL) obtained from diabetic patients was low compared with that from healthy subjects, though the compositions were similar in both VLDL. Percentages of the large size VLDL decreased and those of the small size VLDL increased after the incubation with LPL. At the same time, on polyacrylamide gel disk electrophoresis, the smaller catabolic products from these VLDL appeared at a similar position to that of low density lipoproteins (LDL) and at the running front where high density lipoproteins (HDL) had migrated. The amount of the small size VLDL and the LDL-like lipoproteins produced from diabetic VLDL were less than those from normal VLDL and inversely correlated with the percent decrease of the large original size VLDL. This fact suggests that VLDL from diabetic patients are poor substrates for LPL compared with normal VLDL.
Saheki
S
S
Department of Laboratory and Clinical Medicine, Ehime University Medical School, Osaka, Japan.
Hitsumoto
Y
Y
Murase
M
M
Takeuchi
N
N
Uchida
K
K
D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D014461:Ultracentrifugation
D008079:Lipoproteins, VLDL; D008071:Lipoprotein Lipase