Evaluation of the viability of the canine cadaver lung for transplantation.
We evaluated the viability of the cadaver lung and the effect of lung inflation with 100% oxygen using a canine allotransplantation model. Donor animals were killed by potassium chloride (KCl) injection and were kept at room temperature until lung extraction. The animals were divided into the following 3 groups: group 1 (n = 6) in which the donor lungs were retrieved 2h after sacrifice, group 2 (n = 6) in which the donor lungs were retrieved 3h after sacrifice, and group 3 (n = 6) in which the donor lungs were retrieved 3h after sacrifice as in group 2 except that they were kept inflated for 3h with 100% oxygen using a double lumen endotracheal tube. Heparin was not given and lungs were not flushed with preservation solution. After left lung transplantation, the transplanted lung function including gas exchange and pulmonary hemodynamics was assessed for 6h by ligating the right pulmonary artery of the recipient animals. All 6 animals in groups 1 and 3 survived for 6 h with excellent lung function. Only 2 of 6 animals in group 2 survived for 6h with poor lung function. These results led us to conclude the following: a) the cadaver lung kept at room temperature for 2h might be available for lung transplantation, and b) when the cadaver lung is inflated with 100% oxygen, the length of safe ischemic time could be prolonged up to 3h.
Kayano
K
K
Second Department of Surgery, Okayama University Medical School, Japan.
Date
H
H
Uno
K
K
Shimizu
N
N
Teramoto
S
S
D000818:Animals; D002102:Cadaver; D004285:Dogs; D006439:Hemodynamics; D008168:Lung / Q000473:pathology / Q000502:physiology; D016040:Lung Transplantation; D011652:Pulmonary Circulation; D011659:Pulmonary Gas Exchange; D013696:Temperature; D013997:Time Factors; D014022:Tissue Survival