Kinetics of luminol-enhanced chemiluminescence induced in murine splenocytes and bone marrow cells by various stimulating agents.
The luminol-amplified chemiluminescence (CL) activity of murine splenocytes and bone marrow cells was investigated and compared. Phagocytosis was activated by adding starch grains, opsonized zymosan particles (OZP), or phorbol myristate acetate (PMA) to cell suspensions. CL was studied within a concentration range of 3.13 x 10(6)-1.25 x 10(8) splenocytes/ml, and 1.56 x 10(6)-6.25 x 10(7) bone marrow cells/ml. The CL reaction intensity was increasing with rising cell concentration throughout the whole range studied. Starch grains and OZP elicited a unimodal kinetics of the CL response with a single peak after 10 min for splenocytes and 13-16 min for bone marrow cells. PMA activation induced a bimodal reaction with the first peak appearing after 3 min for either of the cell populations, and the second maximal peak being recorded after 6-7 min for splenocytes and 9-10 min for bone marrow cells.
Cíz
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M
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Lojek
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D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D005260:Female; D008163:Luminescent Measurements; D008165:Luminol; D051379:Mice; D008807:Mice, Inbred BALB C; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D017382:Reactive Oxygen Species; D013154:Spleen / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate; D015054:Zymosan / Q000276:immunology*
D009895:Opsonin Proteins; D017382:Reactive Oxygen Species; D008165:Luminol; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate