A cell line that expresses a reporter gene in response to infection by Sindbis virus: a prototype for detection of positive strand RNA viruses.
We describe a stably transformed cell line (BHKSINLuc2) that contains a defective Sindbis virus genome under the control of a Rous sarcoma virus promoter and the luciferase gene downstream of the viral subgenomic RNA promoter. This cell line expresses high levels of luciferase activity following infection with Sindbis virus and provides a sensitive assay for titering variants of Sindbis virus that lack the structural protein genes, in particular, Sindbis virus replicons that express heterologous proteins. Cell lines such as this may be of value for detection of positive-strand RNA viruses.
Olivo
P D
PD
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110-1093.
Frolov
I
I
Schlesinger
S
S
D000524:Alphavirus / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D002461:Cell Line, Transformed; D006224:Cricetinae; D017930:Genes, Reporter; D012367:RNA, Viral / Q000032:analysis; D012093:Replicon; D012845:Sindbis Virus / Q000235:genetics / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods
D012367:RNA, Viral