5-Hydroxyeicosatetraenoic acid biosynthesis by gestational tissues: effects of inflammatory cytokines.
OBJECTIVE
Our purpose was to determine whether inflammatory cytokines can modulate the production of arachidonate lipoxygenase metabolites by gestational tissues.


METHODS
Primary cultures of amnion, chorion, and decidual cells were incubated in the presence and absence of interleukin-1 beta, tumor necrosis factor-alpha, and interleukin-6. Supernatants were assayed for leukotriene B4, leukotriene C4, 5-, 12- and 15-hydroxyeicosatetraenoic acid by means of specific radioimmunoassays. Cellular proteins were determined.


RESULTS
5-Hydroxyeicosatetraenoic acid production was significantly increased by interleukin-1 beta and interleukin-6 treatment in amnion, chorion, and decidual cells. Tumor necrosis factor-alpha elicited a modest increase, which was not statistically significant, in mean 5-hydroxyeicosatetraenoic acid production by these cells. Production rates of leukotrienes B4 and C4 and 12 and 15-hydroxyeicosatetraenoic acid were not affected by treatment with interleukin-1 beta.


CONCLUSIONS
Inflammatory cytokines generated in response to intrauterine infection may modulate uterine activity by stimulation of 5-hydroxyeicosatetraenoic acid biosynthesis.
Edwin
S S
SS
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
LaMarche
S L
SL
Thai
D
D
Branch
D W
DW
Mitchell
M D
MD
D000650:Amnion / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D001095:Arachidonic Acids / Q000096:biosynthesis; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D003656:Decidua / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005321:Extraembryonic Membranes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000502:physiology*; D015850:Interleukin-6 / Q000502:physiology; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid