A simple method to assess in vivo repair of ultraviolet radiation-induced lesions of specific DNA sequences of restriction sites.
Based on the direct relationship between ultraviolet irradiation of DNA and its susceptibility to restriction endonucleases, we have devised a simple method to quantify in vivo damage and repair of selected restriction sites. The simplicity and power of the method were demonstrated with HindIII restriction endonuclease and pAc360-501-beta-gal plasmid in UV-irradiated Escherichia coli cells. The large number of available restriction endonucleases makes the method quite flexible. This method provides a simple and inexpensive means to screen mutagenic and antimutagenic drugs that interact with the DNA repair mechanism.
Kovács
A
A
Institute of Enzymology, Hungarian Academy of Sciences, Budapest.
Gál
P
P
Závodszky
P
P
D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000528:radiation effects; D010957:Plasmids; D014466:Ultraviolet Rays
D004247:DNA; D004262:DNA Restriction Enzymes