Intracellular growth of Legionella pneumophila serogroup 1 monoclonal antibody type 2 positive and negative bacteria.
Epidemiological evidence suggests that monoclonal antibody type 2 positive (MAB 2+) Legionella pneumophila serogroup 1 (LP1) more often causes disease than do MAB 2- isolates, and there is evidence that MAB 2- LP1 grow less well in cells than do MAB 2+ bacteria. We tested the intracellular growth rates of ten randomly selected MAB 2- LP1 isolates, by using guinea-pig alveolar macrophages, and human monocyte-derived macrophages. Save a low virulence control, all ten MAB 2- isolates grew as well in cells as a virulent MAB 2+ isolate. Heterogeneity of MAB 2- LP1 growth in cells exists, making poor intracellular growth an unlikely explanation for why MAB 2+ LP1 appear to cause disease more often.
Edelstein
P H
PH
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-4283.
Edelstein
M A
MA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D006168:Guinea Pigs; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification / Q000254:growth & development* / Q000276:immunology; D016676:Macrophages, Alveolar / Q000382:microbiology*; D009000:Monocytes / Q000382:microbiology*
D000911:Antibodies, Monoclonal