title: [DNA-dependent RNA-polymerase II from human placental nuclei].
abstract: The procedure for isolation and purification of RNA polymerase II from human placenta nuclei is described. The sensitivity of the enzyme to alpha-amanitin, bivalent cations, ionic strength and glycerol concentration in vitro has been studied. Eleven subunits of the RNA polymerase II molecule have been identified by gel electrophoresis. An optimal RNA polymerase II assay mixture has been developed.
authors.forename: V M
authors.initials: VM
authors.lastname: Sedova
authors.forename: V I
authors.initials: VI
authors.lastname: Vorob'ev
mesh_terms: D002467:Cell Nucleus / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D012319:RNA Polymerase II / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D012319:RNA Polymerase II
vernacular_title: DNK-zavisimaia RNK-polimeraza II iz iader platsenty cheloveka.