Simultaneous subtyping of group specific component and esterase D by isoelectric focusing on agarose gels.
A quick, sensitive and economical technique has been developed to subtype GC and ESD simultaneously on the same agarose IEF gel. This method could be a useful tool for forensic application.
Miller
S A
SA
Analytical Genetic Testing Center, Inc., Denver, CO.
Stern
C M
CM
Schanfield
M S
MS
D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D005554:Forensic Medicine; D007525:Isoelectric Focusing / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D012685:Sepharose; D013997:Time Factors; D014809:Vitamin D-Binding Protein / Q000032:analysis*
D014809:Vitamin D-Binding Protein; D012685:Sepharose; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human