title: Stimulation of renal interstitial bradykinin by sodium depletion.
abstract: The ability to measure and detect change in renal bradykinin in situ would allow study of relations between local kinin production and renal function in hypertensive or diabetic disorders. A new renal interstitial microdialysis technique allowed collections of renal subcapsular interstitial fluid 2 weeks after microdialysis probe placement in conscious dogs (n = 5) on a normal sodium diet (50 mEq/day) and for 5 subsequent days on low sodium intake (10 mEq/day). Although interstitial bradykinin measured by radioimmunoassay (RIA) was undetectable (< 0.08 pg/min) during normal sodium intake, it was detectable (0.34 +/- 0.02 pg/min) after 1 day of low sodium. The kinin level at the end of the 5 subsequent days on low sodium was 1.94 +/- 0.09 pg/min (P < .01). The data show that renal interstitial kinin can be measured in situ. Further, a low sodium diet can rapidly increase interstitial kinin in the conscious dog.
authors.affiliation: Department of Internal Medicine, University of Virginia, Charlottesville.
authors.forename: H M
authors.initials: HM
authors.lastname: Siragy
authors.forename: A A
authors.initials: AA
authors.lastname: Jaffa
authors.forename: H S
authors.initials: HS
authors.lastname: Margolius
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000378:metabolism*; D004285:Dogs; D005260:Female; D007668:Kidney / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D012982:Sodium, Dietary / Q000008:administration & dosage*
chemical_list: D012982:Sodium, Dietary; D012964:Sodium; D001920:Bradykinin