Funnel-well SDS-PAGE: a rapid technique for obtaining sufficient quantities of low-abundance proteins for internal sequence analysis.
We report a modified sodium dodecyl sulfate polyacrylamide gel electrophoresis method that permits up to a 60-fold concentration factor, without significant loss of protein. This method leads to very efficient concentration of low-abundance proteins from partially purified fractions or very dilute protein solution. Furthermore, it permits in situ enzymatic digestion and consequently increases the probability of obtaining a suitable internal sequence.
Lombard-Platet
G
G
Laboratoire de Biologie Moléculaire et Cellulaire, Ecole Normale Supérieure de Lyon.
Jalinot
P
P
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D017421:Sequence Analysis
D011506:Proteins