The use of dietary loading of 133Cs as a potassium substitute in NMR studies of tissues.
133Cs NMR chemical shifts and relaxation times have been measured for tissue samples in vitro and in vivo from rats which have been fed on a high cesium, low potassium diet, which leads to a predominantly intracellular distribution of this ion, similar to that of K+. The high sensitivity, large chemical shift range, and narrow linewidths of 133Cs, compared with 39K, allow chemical shift differences to be observed between tissues, and in subcellular organelles such as mitochondria. For example, in vitro tissue chemical shifts, relative to 150 mM CsCl, are 1.06 +/- 0.11 ppm for liver, 0.02 +/- 0.05 ppm for brain, 1.76 +/- 0.20 ppm for erythrocytes, and -0.13 +/- 0.02 ppm for plasma. T1 and spin-echo T2 values range from 1.26 +/- 0.05 s (T1), and 0.028 +/- 0.006 s (T2) for liver, to 6.49 +/- 0.19 s and 1.12 +/- 0.03 s for plasma. 133Cs relaxation times show the same relative trends between tissues as are observed in 39K tissue studies.
Shehan
B P
BP
Victorian College of Pharmacy, Monash University, Parkville, Australia.
Wellard
R M
RM
Adam
W R
WR
Craik
D J
DJ
D000818:Animals; D002586:Cesium / Q000493:pharmacokinetics; D002587:Cesium Isotopes; D004032:Diet; D004912:Erythrocytes / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D017419:Potassium, Dietary / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D002587:Cesium Isotopes; D017419:Potassium, Dietary; D002586:Cesium