title: Localization of stress protein-70 in ovine corpora lutea during prostaglandin-induced luteolysis.
abstract: Accumulation of heat shock (stress) protein-70 (HSP-70) was assessed in corpora lutea of sheep obtained after in vivo administration of a luteolytic dose of prostaglandin (PG) F2 alpha. A quantitative immunofluorescence technique was used to localize inducible HSP-70 production to specific cell-types. The number and intensity of immunostained large luteal cells increased within 2 h of injection of PGF2 alpha. A drop in luteal progesterone concentrations (functional regression) was not manifested until 4 h post-treatment. A dramatic increase in intensely-stained mononuclear leukocytes was observed in luteal tissues at 16 h, when glandular weights had begun to diminish (structural regression). Stress proteins could mediate intracellular protein processing and cell-surface autoimmune mechanisms underlying luteal regression.
authors.affiliation: Department of Animal Science, University of Wyoming, Laramie 82071.
authors.forename: L A
authors.initials: LA
authors.lastname: McPherson
authors.forename: E A
authors.initials: EA
authors.lastname: Van Kirk
authors.forename: W J
authors.initials: WJ
authors.lastname: Murdoch
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000378:metabolism*; D003341:Luteolysis / Q000187:drug effects / Q000502:physiology*; D009929:Organ Size; D011374:Progesterone / Q000378:metabolism; D012756:Sheep
chemical_list: D006360:Heat-Shock Proteins; D011374:Progesterone; D015237:Dinoprost