T cell activation by conagenin in mice.
Conagenin (CNG), a low molecular immunomodulator, enhanced incorporation of [3H]thymidine into T cells activated by concanavalin A but did not to non-activated T cells. The culture supernatants of activated T cells treated with CNG enhanced incorporation of [3H]thymidine into cytokine dependent cell lines, CTLL-2 and IC-2 cells. This indicates that CNG exclusively acts on activated T cells and stimulates them to promote DNA synthesis and to produce lymphokines, which may include T cell growth factors and hematopoietic growth factors. These activities were also observed with T cells taken from mice given CNG.
Kawatsu
M
M
Institute for Chemotherapy, M.C.R.F., Shizuoka, Japan.
Yamashita
T
T
Osono
M
M
Ishizuka
M
M
Takeuchi
T
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis; D051379:Mice; D012694:Serine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D008222:Lymphokines; C069645:conagenin; D012694:Serine; D013936:Thymidine