Rapid identification of some Leptospira isolates from cattle by random amplified polymorphic DNA fingerprinting.
We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Australian cattle. Each serovar and genotype had a unique RAPD profile. Of 26 field isolates of Leptospira, 23 were identified as hardjo genotype bovis subtype A, 2 were identified as zanoni, and 1 was identified as pomona by RAPD fingerprinting, and their types were confirmed by cross-absorption agglutination and restriction enzyme analysis.
Corney
B G
BG
Animal Research Institute, Queensland Department of Primary Industries, Moorooka, Australia.
Colley
J
J
Djordjevic
S P
SP
Whittington
R
R
Graham
G C
GC
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D005784:Gene Amplification; D007919:Leptospira / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial