Cloning of cDNA and analysis of the gene for mouse angiotensin II type 2 receptor.
A cDNA clone encoding the mouse angiotensin receptor subtype 2 (AT2 receptor) was isolated from a mouse fetal cDNA library. Mouse AT2 receptor full-length cDNA contains a 1089-base-pair open reading frame which encodes a protein of 363 amino acid residues. Hydropathy analysis reveals seven putative transmembrane domains. Specific binding of [125I]CGP42112A was demonstrated in membranes of COS-7 cells transfected with the cDNA. Scatchard analysis and ligand displacement profile were typical of the AT2 receptor. Northern blot and reverse transcription-polymerase chain reaction analyses demonstrated that AT2 receptor mRNA was expressed abundantly in fetus and in a lesser amount in adult brain. Analysis of the mouse AT2 receptor gene reveals that the mouse AT2 receptor locus exists as a single copy in the genome, and that the gene does not contain introns in the coding region.
Nakajima
M
M
Falk Cardiovascular Research Center, Stanford University School of Medicine, California 94305-5246.
Mukoyama
M
M
Pratt
R E
RE
Horiuchi
M
M
Dzau
V J
VJ
D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism*; D005333:Fetus; D015723:Gene Library; D007438:Introns; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D011945:Receptors, Angiotensin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II