Messenger RNA levels of five genes located at chromosome 11q13 in B-cell tumors with chromosome translocation t(11;14)(q13;q32).
To identify genes activated by chromosome translocation t(11;14)(q13;q32), mRNA levels of five genes (cyclin D1, EXP1, MB38, HST1, and INT2) at chromosome 11q13 were investigated. The cyclin D1 mRNA increased in BCL-1-rearranged B-cell tumor cell lines SP-49, NOP-2, FLAM-76, KMS-12-PE, and KMS-12-BM cells, while it was not detected in cell lines without the translocation, Raji, U266, and HEL cells. A significant amount of the MB38 mRNA was detected irrelevantly to the translocation in all of these cell lines. The mRNAs of EXP1, HST1, and INT2 were undetectable in these cells. The results suggested that the translocation activates cyclin D1 alone, while the mRNA levels of the other four genes are regulated independently of the translocation.
Akiyama
N
N
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Tsuruta
H
H
Sasaki
H
H
Sakamoto
H
H
Hamaguchi
M
M
Ohmura
Y
Y
Seto
M
M
Ueda
R
R
Hirai
H
H
Yazaki
Y
Y
D015152:Blotting, Northern; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D019938:Cyclin D1; D016213:Cyclins / Q000235:genetics*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D009101:Multiple Myeloma / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D014178:Translocation, Genetic
D016213:Cyclins; D015513:Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D019938:Cyclin D1