title: Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid).
abstract: We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 mumol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 mumol/day.
authors.affiliation: Institute for Medical Biochemistry, University Clinical Center, Belgrade, Serbia.
authors.forename: D
authors.initials: D
authors.lastname: Agbaba
authors.forename: M
authors.initials: M
authors.lastname: Stojanov
authors.forename: S
authors.initials: S
authors.lastname: Rajacic
authors.forename: D
authors.initials: D
authors.lastname: Zivanov-Stakic
authors.forename: N
authors.initials: N
authors.lastname: Majkic-Singh
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000175:diagnosis / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000706:statistics & numerical data; D003720:Densitometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D010673:Pheochromocytoma / Q000175:diagnosis / Q000652:urine; D011786:Quality Control; D012016:Reference Values; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D014642:Vanilmandelic Acid / Q000652:urine*
chemical_list: D014642:Vanilmandelic Acid