Significance of terminal rinse for rat liver preservation.
A terminal rinse (TR) is standard practice in liver preservation with University of Wisconsin solution (UW) to avoid a potassium load. The fact that sodium lactobionate sucrose solution (SLS) is an effective organ preservation solution with a low potassium provided an opportunity to evaluate rat liver preservation without the TR step. Its importance was investigated in 122 rat liver preservation experiments. In study 1, UW and a hydroxyethyl starch-free, modified UW (UWm) were used for 20-hr liver preservation followed by either no TR or Ringer's lactate TR. The 1-week survival was: UW-TR, 2/14; UW-no TR, 1/6; UWm-TR, 0/6; UWm-no TR, 5/5 (P < 0.01). In study 2, livers were stored for 30 hr in SLS, UW, UWm, and UWm + chlorpromazine 5 mg/L, all without a TR. Nine of 11 rats survived 7 days after SLS, but there were no survivors in the other groups (P < 0.05). Study 3 compared no TR with TR with SLS, Ringer's lactate (RL), or a modified Carolina rinse (CRm) after 30-hr SLS preservation. Survival, serum aspartate aminotransferase and alanine aminotransferase, and histology were assessed. One-week survival of 9/11 rats in no TR was significantly better than in the other groups (3/14 in TR-SLS, 0/8 in TR-RL, and 0/14 in TR-CRm, P < 0.01). The values of aspartate aminotransferase (mean +/- SE) 3 hr after transplantation were 1862 +/- 439 U/L, 3334 +/- 817 U/L, 6591 +/- 1944 U/L, and 7028 +/- 1704 U/L, respectively, in no TR, TR-SLS, TR-RL, and TR-CRm. There were significant differences both in aspartate aminotransferase and alanine aminotransferase between no-TR and each of TR-RL and TR-CRm (P < 0.05). Liver specimens from rats killed 3 hr after OLT showed only mild injury in the no TR group and severe injury in the remaining groups. We conclude that a terminal rinse is harmful in rat liver preservation.
Egawa
H
H
California Pacific Medical Center, and Medical Research Institute, San Francisco.
Esquivel
C O
CO
Wicomb
W N
WN
Kennedy
R G
RG
Collins
G M
GM
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002746:Chlorpromazine; D004187:Disaccharides; D005069:Evaluation Studies as Topic; D005978:Glutathione; D006085:Graft Survival; D007328:Insulin; D008099:Liver / Q000293:injuries / Q000473:pathology / Q000502:physiology; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology; D008297:Male; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D013395:Sucrose / Q000031:analogs & derivatives
D004187:Disaccharides; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; C067074:sodium lactobionate sucrose solution; D013395:Sucrose; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D002746:Chlorpromazine