Growth factors and kidney development.
The formation of the metanephric kidney is dependent upon the timed and sequential expression of a number of polypeptide growth factors. To shed light on the participation of members of the insulin-like growth factor (IGF) and epidermal growth factor/transforming growth factor-alpha (EGF/TF-alpha) families, we measured the synthesis of IGF-I, IGF-II, EGF and TGF-alpha by developing rat metanephroi in organ culture and determined the effect of anti-growth factor antibodies on growth and development. IGF-I, IGF-II and TGF-alpha were produced by metanephroi and released into culture media. We could detect no EGF. Inclusion of anti-IGF-I, anti-IGF-II, anti-IGF-II receptor or anti-TGF-alpha antibodies in organ cultures inhibited growth and development of metanephroi. Our findings suggest that both members of the IGF family and TGF-alpha are produced within the developing metanephros and promote renal organogenesis.
Hammerman
M R
MR
George M. O'Brien Kidney and Urological Diseases Center, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Rogers
S A
SA
Ryan
G
G
D000818:Animals; D003470:Culture Media; D006133:Growth Substances / Q000096:biosynthesis / Q000502:physiology*; D007668:Kidney / Q000196:embryology* / Q000378:metabolism*; D009399:Nephrons / Q000196:embryology / Q000378:metabolism; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D003470:Culture Media; D006133:Growth Substances; D012333:RNA, Messenger