title: Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles.
abstract: The cycle of nucleotide exchange and hydrolysis by a small GTP-binding protein, ADP-ribosylation factor (ARF), helps to provide vectoriality to vesicle transport. Coat assembly is triggered when ARF binds GTP, initiating transport vesicle budding, and coat disassembly is triggered when ARF hydrolyzes GTP, allowing the uncoated vesicle to fuse.
authors.affiliation: Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.
authors.forename: G
authors.initials: G
authors.lastname: Tanigawa
authors.forename: L
authors.initials: L
authors.lastname: Orci
authors.forename: M
authors.initials: M
authors.lastname: Amherdt
authors.forename: M
authors.initials: M
authors.lastname: Ravazzola
authors.forename: J B
authors.initials: JB
authors.lastname: Helms
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
mesh_terms: D020727:ADP-Ribosylation Factors; D000818:Animals; D016466:CHO Cells; D002352:Carrier Proteins / Q000378:metabolism*; D002474:Cell-Free System; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008561:Membrane Fusion; D008854:Microscopy, Electron; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors