title: Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease.
abstract: We report the cloning and characterization of two outer surface proteins (Osps), designated OspE and OspF, from strain N40 of Borrelia burgdorferi, the spirochetal agent of Lyme disease. The ospE and ospF genes are structurally arranged in tandem as one transcriptional unit under the control of a common promoter. The ospE gene, located at the 5' end of the operon, is 513 nucleotides in length and encodes a 171-amino-acid protein with a calculated molecular mass of 19.2 kDa. The ospF gene, located 27 bp downstream of the stop codon of the ospE gene, consists of 690 nucleotides and encodes a protein of 230 amino acids with a calculated molecular mass of 26.1 kDa. Pulsed-field gel electrophoresis showed that the ospE and ospF genes are located on a 45-kb plasmid. Comparison of the leader sequences of OspE and OspF with those of the four known B. burgdorferi Osps (OspA, OspB, OspC, and OspD) reveals a hydrophobic domain and a consensus cleavage sequence (L-X-Y-C) recognized by signal peptidase II, and [3H]palmitate labeling shows that OspE and OspF are lipoproteins. Immunofluorescence studies demonstrated that both the OspE and OspF proteins are surface exposed. These features are consistent with the finding that OspE and OspF are B. burgdorferi surface lipoproteins.
authors.affiliation: Section of Immunobiology, Howard Hughes Medical Institute, New Haven, Connecticut.
authors.forename: T T
authors.initials: TT
authors.lastname: Lam
authors.forename: T P
authors.initials: TP
authors.lastname: Nguyen
authors.forename: R R
authors.initials: RR
authors.lastname: Montgomery
authors.forename: F S
authors.initials: FS
authors.lastname: Kantor
authors.forename: E
authors.initials: E
authors.lastname: Fikrig
authors.forename: R A
authors.initials: RA
authors.lastname: Flavell
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins; D001483:Base Sequence; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D003062:Codon; D005455:Fluorescent Antibody Technique; D005798:Genes, Bacterial; D006801:Humans; D008074:Lipoproteins; D008193:Lyme Disease / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009876:Operon; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012995:Solubility
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003062:Codon; D008074:Lipoproteins; C084998:OspE protein, Borrelia burgdorferi; C084999:OspF protein, Borrelia burgdorferi