Reduction of immunoreactivity of bovine serum albumin conjugated with comb-shaped polyethylene glycol derivatives.
Bovine serum albumin (BSA) was chemically modified with two types of comb-shaped copolymers of polyethylene glycol derivative and maleic anhydride, one with the molecular weight of 13,000 (activated PM13) and the other with 100,000 (activated PM100), to form PM13- and PM100-BSA. The immunoreactivity of BSA was markedly reduced by coupling with each modifier and was completely lost when 30% or 20% of amino groups in BSA were modified with activated PM13 or PM100, respectively. The esterase activity of PM13- and PM100-BSA without immunoreactivity were retained 63% and 93% of non-modified one, respectively. These results were discussed with those of modified-asparaginases(1).
Sasaki
H
H
Department of Materials Science and Technology, Toin University of Yokohama, Japan.
Ohtake
Y
Y
Matsushima
A
A
Hiroto
M
M
Kodera
Y
Y
Inada
Y
Y
D001215:Asparaginase / Q000737:chemistry / Q000276:immunology; D008299:Maleic Anhydrides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011233:Precipitin Tests; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology*
D008299:Maleic Anhydrides; C054144:polyoxyethylene allylmethyl diether-maleic anhydride copolymer; C084496:polyoxyethylene (2-methyl-2-propenyl) methyl diether-maleic anhydride copolymer; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D001215:Asparaginase