Relationship between blood pressure and smooth muscle tone in aortae of hypertensive rats: roles of [Ca2+].
Spontaneously developed tension (active tone) and intracellular Ca2+ level of aortae from spontaneously hypertensive rats (SHR), stroke-prone SHR (SHRSP), malignant SHRSP (M-SHRSP) and control Wistar Kyoto rats (WKY) were compared. Systolic blood pressure of WKY, SHR, SHRSP and M-SHRSP was 130 mmHg, 200 mmHg, 250 mmHg and 260 mmHg, respectively. Preparations from all strains of spontaneously hypertensive rats exhibited active tone which was abolished by the removal of extracellular Ca2+ or by the application of verapamil. The active tone was greater in the order of aortae from SHR < SHRSP < M-SHRSP. Intracellular Ca2+ level measured by Fura-2 method decreased by the removal of extracellular Ca2+. The degree of the decrease was greater as the blood pressure of the rats increased, indicating the greater elevation of intracellular Ca2+ level in the pressure of extracellular Ca2+. A correlation was obtained between the active tone, intracellular Ca2+ level and blood pressure. Thus, it was demonstrated that the development of the active tone is brought about by the changes in Ca2+ influx of smooth muscle cell membrane and the degree of the change is positively related to the degree of hypertension.
Sasaki
F
F
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Japan.
Osugi
S
S
Shimamura
K
K
Sunano
S
S
D000818:Animals; D001011:Aorta / Q000503:physiopathology*; D001794:Blood Pressure; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D006973:Hypertension / Q000503:physiopathology*; D066298:In Vitro Techniques; D009119:Muscle Contraction; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D014700:Verapamil / Q000494:pharmacology
D014700:Verapamil; D002118:Calcium