title: Inhibition of Mycoplasma gallisepticum growth and attachment to chick tracheal rings by antibodies to a 64-kilodalton membrane protein of M. gallisepticum.
abstract: A Mycoplasma gallisepticum (MG) strain R protein of 64 kilodaltons (p64) was partially digested from the surface of the bacterium by trypsin. Monospecific polyclonal anti-p64 IgG inhibited attachment of MG to chick tracheal rings by as much as 69%. However, trypsin treatment of viable MG cells did not reduce attachment to tracheal rings or hemagglutination titer. Anti-p64 IgG inhibited growth of MG strain R in broth and on solid media, inhibited the uptake of radiolabeled thymidine, but did not inhibit hemagglutination. Anti-p64 IgG inhibited growth of eight MG strains on solid medium. The degree of growth inhibition varied widely depending on the strain and correlated positively with the reported virulence of the MG strains with one exception (A5969). An IgG monoclonal antibody to p64 (MyG 001) inhibited growth of MG strain R on solid and in broth media. The strong attachment-inhibition activity of anti-p64 IgG may result from its growth-inhibiting activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of MG strains suggested that p64 is expressed in higher amounts in vitro in virulent strains (R, S6) than in strains of low virulence (F, M876, K503, K703, K730). P64 should be used to immunize chickens to determine if it can stimulate a growth and attachment-inhibiting response in the respiratory tract.
authors.affiliation: Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
authors.forename: A P
authors.initials: AP
authors.lastname: Avakian
authors.forename: D H
authors.initials: DH
authors.lastname: Ley
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D001428:Bacterial Vaccines / Q000302:isolation & purification; D002645:Chickens; D006386:Hemagglutination Tests; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology; D008970:Molecular Weight; D009174:Mycoplasma / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control; D014132:Trachea / Q000382:microbiology; D014357:Trypsin; D015164:Vaccines, Inactivated / Q000302:isolation & purification; D014774:Virulence / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D008565:Membrane Proteins; D015164:Vaccines, Inactivated; D014357:Trypsin