Identification of a new serine kinase that activates NF kappa B by direct phosphorylation.
A novel serine kinase, named nuclear factor kappa B (NF kappa B) kinase, has been shown to be associated with the NF kappa B.I kappa B complex in the cytosol of human primary T lymphocytes. It activates the DNA binding activity of NF kappa B by directly phosphorylating its p65 and p50 subunits. There is no evidence that it phosphorylates I kappa B. Experiments with inhibitors and antisera showed that it is distinct from other known serine kinases such as mitogen-activated protein kinase or Mos. It has an apparent molecular size of 43 kDa determined by the substrate binding assay. This kinase might have a central role in mediating various signals to NF kappa B.
Hayashi
T
T
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
Sekine
T
T
Okamoto
T
T
D000255:Adenosine Triphosphate / Q000378:metabolism; D001483:Base Sequence; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D013379:Substrate Specificity; D013601:T-Lymphocytes / Q000201:enzymology / Q000378:metabolism; D051841:Transcription Factor RelB; D014157:Transcription Factors; D000097782:NF-kappaB-Inducing Kinase
D000255:Adenosine Triphosphate; D004247:DNA; D004268:DNA-Binding Proteins; D016328:NF-kappa B; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D051841:Transcription Factor RelB; D014157:Transcription Factors; D000097782:NF-kappaB-Inducing Kinase; C497593:RELB protein, human