A single chicken anemia virus protein induces apoptosis.
Chicken anemia virus (CAV) causes cytopathogenic effects in chicken thymocytes and cultured transformed mononuclear cells via apoptosis. Early after infection of chicken mononuclear cells, the CAV-encoded protein VP3 exhibits a finely granular distribution within the nucleus. At a later stage after infection, VP3 forms aggregates. At this point, the cell becomes apoptotic and the cellular DNA is fragmented and condensed. By immunogold electron microscopy VP3 was shown to be associated with apoptotic structures. In vitro, expression of VP3 induced apoptosis in chicken lymphoblastoid T cells and myeloid cells, which are susceptible to CAV infection, but not in chicken embryo fibroblasts, which are not susceptible to CAV. Expression of a C-terminally truncated VP3 induced much less pronounced apoptosis in the chicken lymphoblastoid T cells.
Noteborn
M H
MH
Laboratory for Molecular Carcinogenesis, Sylvius Laboratory, Leiden University, The Netherlands.
Todd
D
D
Verschueren
C A
CA
de Gauw
H W
HW
Curran
W L
WL
Veldkamp
S
S
Douglas
A J
AJ
McNulty
M S
MS
van der EB
A J
AJ
Koch
G
G
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D036022:Capsid Proteins; D002467:Cell Nucleus / Q000648:ultrastructure; D017727:Chicken anemia virus; D002645:Chickens; D003588:Cytopathogenic Effect, Viral; D004249:DNA Damage; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D036022:Capsid Proteins