Antihypertensive principles from the leaves of Melastoma candidum.
Three active principles were isolated from the leaf of Melastoma candidum using the screening of hypotensive effects on spontaneously hypertensive rats (SHR). Intravenous injection of castalagin, procyanidin B-2, or helichrysoside into SHR lowered the mean blood pressure in a dose-dependent manner, with helichrysoside being the most potent compound. Plasma noradrenaline (NA) levels, both basal in SHR and elevated in normal rats through cold-stress stimulation, were attenuated by these compounds in a way which was not influenced by adrenalectomy. Decrease of NA release from sympathetic nerves was assumed to be responsible. Moreover, the hypertensive effect of various vasoconstrictors in anesthetized rats was reduced by helichrysoside. The same results were also observed in castalagin or procyanidin B-2 treated animals. The results indicate that the three principles possess the ability to lower blood pressure through a decrease of sympathetic tone as well as due to direct vasodilatation in SHRs.
Cheng
J T
JT
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, Republic of China.
Hsu
F L
FL
Chen
H F
HF
D000818:Animals; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D044946:Biflavonoids; D001713:Biphenyl Compounds; D002392:Catechin / Q000302:isolation & purification / Q000494:pharmacology*; D002396:Catechols / Q000302:isolation & purification / Q000494:pharmacology*; D002867:Chromones / Q000302:isolation & purification / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D047348:Hydrolyzable Tannins; D015394:Molecular Structure; D009638:Norepinephrine / Q000097:blood; D044945:Proanthocyanidins; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D000959:Antihypertensive Agents; D044946:Biflavonoids; D001713:Biphenyl Compounds; D002396:Catechols; D002867:Chromones; D004365:Drugs, Chinese Herbal; D047348:Hydrolyzable Tannins; D044945:Proanthocyanidins; C479578:procyanidin B; C084709:vescalagin; C017674:procyanidin; C084710:helichrysoside; D002392:Catechin; D009638:Norepinephrine