Cloning of a mouse cDNA encoding DNA polymerase delta: refinement of the homology boxes.
A mouse DNA polymerase delta (Pol delta)-encoding cDNA (pol delta) was isolated by PCR amplification and cDNA library screening. The sequenced cDNA has a length of 3386 bp and the open reading frame (ORF) encodes a protein of 1105 amino acids (aa) with an M(r) of 123,743. The aa identity to the proteins encoded by the corresponding cDNA from Bos taurus (93%) and Homo sapiens (92%) is very high. The identity to the Pol delta from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Plasmodium falciparum is around 50%. An aa comparison between all available Pol delta sequences reveals several common structural motifs. Polyclonal antibodies raised against a 31-aa synthetic peptide deduced from the ORF specifically recognize Pol delta polymerases from human cells and calf thymus in an immunoblot.
Cullmann
G
G
Institut für Veterinärbiochemie, Universität Zürich-Irchel, Switzerland.
Hindges
R
R
Berchtold
M W
MW
Hübscher
U
U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D004258:DNA Polymerase III; D018076:DNA, Complementary; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase