Histone acetylation and gene induction in human cells.
An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet derived growth factor B chain (PDGF-B), a gene which is induced to strong expression upon differentiation. A high level of acetylation was observed before gene induction and no change seen following induction. This implies that core histone acetylation is an essential precondition for transcription.
Clayton
A L
AL
Biophysics Laboratories, University of Portsmouth, UK.
Hebbes
T R
TR
Thorne
A W
AW
Crane-Robinson
C
C
D000107:Acetylation; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002843:Chromatin; D006657:Histones; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D004247:DNA; D011505:Protein-Tyrosine Kinases; D013755:Tetradecanoylphorbol Acetate