Effects of platelet activating factor on human sperm function in vitro.
The direct effects of platelet activating factor (PAF) and the specific PAF receptor antagonist, CV-3988, on the fertilizing ability of human spermatozoa were investigated. PAF (10(-7)-10(-11) M) increased the human sperm penetration rates in a sperm penetration assay at all doses > 10(-11) M. In contrast, treatment of the spermatozoa with 10(-5) CV-3988 caused a significant decrease in human sperm penetration of zona-free hamster oocytes and adversely affected sperm motility after 24 h of incubation. This suppression was reversed by the addition of PAF. The acrosome reaction was also enhanced by PAF treatment of spermatozoa but this effect was not observed in calcium-free medium. While 10(-5) M CV-3988 decreased the acrosome reaction, the inhibition was also reversed by the addition of PAF. These results suggest that PAF may have a direct role in the fertilizing capacity of human spermatozoa. These findings also suggest that PAF may have a clinical application in an in-vitro fertilization programme.
Sengoku
K
K
Department of Obstetrics and Gynaecology, Asahikawa Medical College, Japan.
Tamate
K
K
Takaoka
Y
Y
Ishikawa
M
M
D000177:Acrosome / Q000187:drug effects; D000818:Animals; D006224:Cricetinae; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes; D010742:Phospholipid Ethers / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*
C029271:O-deacetyl platelet activating factor; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; C037913:CV 3988