Induction of chicken avidin and related mRNAs after bacterial infection.
Inflammation-mediated avidin gene expression in the chicken was studied using hybridization analysis, polymerase chain reaction (RT-PCR) and sequencing. The results indicate the presence of avidin mRNA in the oviduct and intestine after Escherichia coli infection. The mRNA for the inflammation-induced avidin was mainly encoded by the avidin gene, but the avidin-related genes, avr2 and avr3, were also transcribed at a low level in the oviduct and intestine, respectively. Because avidin is also induced in the chicken oviduct by progesterone, our results indicate a multifactorial regulation of avidin gene expression.
Kunnas
T A
TA
University of Tampere, Department of Biomedical Sciences, Finland.
Wallén
M J
MJ
Kulomaa
M S
MS
D000818:Animals; D001360:Avidin / Q000096:biosynthesis*; D001483:Base Sequence; D002645:Chickens; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D004927:Escherichia coli Infections / Q000378:metabolism*; D015870:Gene Expression; D007249:Inflammation; D007413:Intestinal Mucosa / Q000378:metabolism; D008969:Molecular Sequence Data; D010057:Oviducts / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis*; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D001360:Avidin