Concordance between karyotyping and in situ hybridization procedures in the detection of monosomy 9 in bladder cancer.
Twenty-three cases of transitional cell carcinoma (TCC) with a diploid model chromosome count as selected after karyotyping were analyzed by fluorescence in situ hybridization (FISH), using a probe for the heterochromatic region of chromosome 9. A monosomy for chromosome 9 was detected in 50% by karyotyping and after FISH in 52% of the cases. A full concordance between FISH and conventional karyotyping was found. We concluded that FISH can be reliably applied to interphase nuclei of TCC for the detection of numerical chromosome 9 aberrations.
Smeets
W
W
Hospital Pharmacy and Laboratory for Clinical Chemistry, Venray, The Netherlands.
Schapers
R
R
Hopman
A
A
Pauwels
R
R
Ramaekers
F
F
D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D002899:Chromosomes, Human, Pair 9; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009006:Monosomy; D001749:Urinary Bladder Neoplasms / Q000235:genetics*