Chronic ethanol exposure potentiates muscarinic receptor and alpha 2-adrenoceptor-mediated inhibition of cAMP accumulation in PC 12 cells.
The effects of ethanol on receptor-mediated inhibition of cAMP production were investigated in PC 12 cells. The in vitro addition of ethanol enhanced N-ethylcarboxyadenosine (NECA)-stimulated cAMP production without altering the inhibitory action of carbachol or epinephrine. A 4 day exposure of PC 12 cells to ethanol decreased the stimulation of cAMP production by NECA, but increased the inhibition of NECA-stimulated cAMP production by carbachol and epinephrine.
Kim
W K
WK
Department of Pharmacology and Therapeutics, State University of New York at Buffalo 14214-3000.
Hawthorn
M H
MH
Rabin
R A
RA
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D019830:Adenosine-5'-(N-ethylcarboxamide); D000818:Animals; D001665:Binding Sites; D002217:Carbachol / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology; D000431:Ethanol / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects* / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000378:metabolism
D011942:Receptors, Adrenergic, alpha; D011976:Receptors, Muscarinic; D019830:Adenosine-5'-(N-ethylcarboxamide); D000431:Ethanol; D002217:Carbachol; D000242:Cyclic AMP; D000241:Adenosine; D004837:Epinephrine