title: Photo induced surface heparin immobilization.
abstract: This paper describes a novel method providing durable layering of heparin immobilized hydrogels on fabricated devices. The preparation method is based on photochemistry of a dithiocarbamate group that is dissociated into a highly reactive radical pair upon ultraviolet (UV) irradiation. By taking advantage of characteristics of the photo generated radicals, hydrogel formation and its fixation onto a substrate surface were attained. The immobilization of heparin onto poly(ethylene terephtalate) was demonstrated. First, a mixed aqueous solution containing a photoreactive water soluble poly(N,N-dimethylacrylamide-covinylbenzyl N,N-diethyldithiocarbamate) and heparin was coated on the substrate. Subsequent UV irradiation resulted in the simultaneous formation of a heparin immobilized hydrogel and its chemical fixation onto the substrate. No delamination was found after vigorous washing with water. Significant inhibition of platelet adhesion and markedly prolonged blood coagulation times were observed, which are apparently derived from the surface hydrogel, and from released and chemically fixed surface heparin. Thus, it is expected that the photochemical method developed here provides potent antithrombogenicity to artificial organs.
authors.affiliation: Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects; D006493:Heparin / Q000493:pharmacokinetics; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D066298:In Vitro Techniques; D008422:Materials Testing; D010777:Photochemistry; D010973:Platelet Adhesiveness / Q000187:drug effects; D011092:Polyethylene Glycols; D011108:Polymers; D019736:Prostheses and Implants; D013499:Surface Properties
chemical_list: D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D006493:Heparin