[The construction of human genome YAC library and screening by hybridization of membrane high density spotted in array].
The human genomic YAC (Yeast Artificial Chromosome) library was constructed with human white blood cell and the cell line GM1 414 containing 4 X chromosome DNA using pYAC 4 as vector. Twenty thousand YAC clones were obtained with insert size of 400-1000kb. A set of YACs containing entire DMD geneDNA sequence were selected.
Chai
J
J
Institute of Genetics, Fudan University, Shanghai.
Gu
Y
Y
D002460:Cell Line; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization; D014960:X Chromosome