title: The invariant chain induces compact forms of class II molecules localized in late endosomal compartments.
abstract: The invariant chain (Ii) binds to newly synthesized major histocompatibility complex (MHC) class II molecules and is targeted to an acidic compartment where it is degraded. To evaluate its role on the conformation and the subcellular distribution of murine MHC class II molecules we have established stable L cell transfectants expressing class II IAk heterodimers alone or in conjunction with p31 and p41 Ii chains. In these cells, class II molecules were present under three forms: alpha beta heterodimers bearing high mannose carbohydrate moieties, and fully glycosylated alpha beta heterodimers that are sensitive or resistant to sodium dodecyl sulfate dissociation at 20 degrees C. The latter class II molecules called compact heterodimers, were here highly induced in Ii-positive cells. Using in situ iodination of endosomal compartments, class II heterodimers were detected in late endosomal compartments essentially as compact forms in Ii-positive cells, and as non-compact forms in Ii-negative cells. Using confocal microscopy, IAk molecules were located in compartments distinct from early endosomes labeled with transferrin, but partially coincident with vesicles containing fluid-phase markers, and highly coincident with compartments containing large amounts of cathepsins B, D, H, and L in Ii-positive and Ii-negative cells. At the ultrastructural level, class II molecules were mostly present in multivesicular bodies, even without Ii expression. But Ii chains were needed to induce an efficient presentation of the hen egg lysozyme antigen and were sufficient to promote a major conformational change of the late endosomal, and/or lysosomal resident, class II molecules. Ii molecules are presumably playing a chaperoning function favoring the association of peptides with class II molecules in endosomal compartments.
authors.affiliation: Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
authors.forename: M
authors.initials: M
authors.lastname: Humbert
authors.forename: G
authors.initials: G
authors.lastname: Raposo
authors.forename: P
authors.initials: P
authors.lastname: Cosson
authors.forename: H
authors.initials: H
authors.lastname: Reggio
authors.forename: J
authors.initials: J
authors.lastname: Davoust
authors.forename: J
authors.initials: J
authors.lastname: Salamero
mesh_terms: D017951:Antigen Presentation; D000944:Antigens, Differentiation, B-Lymphocyte; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000737:chemistry* / Q000502:physiology*; D006801:Humans; D011487:Protein Conformation
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; C045084:invariant chain