Primary structure of cryX**, the novel delta-endotoxin-related gene from Bacillus thuringiensis spp. galleriae.
A cry-related sequence, designated cryX (EMBL X75019), was localized upstream of cryIG, the delta-endotoxin gene cloned from spp. galleriae of Bacillus thuringiensis and sequenced earlier [(1991) FEBS Lett. 293, 25-28]. Analysis of the cryX complete nucleotide sequence enabled us to explain its virtual crypticity and to reveal the chimeric structure of the genes, cryX and cryIG. The amino acid sequence of 1,151 residues encoded by the continuous reading frame of cryX is similar to the other delta-endotoxins but differs essentially from them.
Shevelev
A B
AB
Institute of Microbial Genetics (VNIIGenetika), Laboratory of Protein Chemistry, Moscow, Russian Federation.
Svarinsky
M A
MA
Karasin
A I
AI
Kogan YaN
Chestukhina
G G
GG
Stepanov
V M
VM
D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D002678:Chimera; D004274:DNA, Recombinant; D004731:Endotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004274:DNA, Recombinant; D004731:Endotoxins; D006460:Hemolysin Proteins; C084957:cryIX protein, Bacillus thuringiensis; C030956:insecticidal crystal protein, Bacillus Thuringiensis