Effect of 5-lipoxygenase inhibitors on the anti-IgE-induced contraction of passively sensitized human bronchus in vitro.
We assessed the effect of 5-lipoxygenase inhibitors on the contraction of human bronchial ring segments passively sensitized with IgE in vitro. Contraction was induced by increasing doses of anti-IgE (0.01-0.5 micrograms/ml) in the presence of the antihistamine mepyramine. Zileuton and MK-886 (10 microM) significantly inhibited the dose-related contraction to anti-IgE as compared with solvent (DMSO). At the highest dose of anti-IgE used, contraction reached 10.3 and 9.6% of the maximal carbachol contraction, respectively, as compared with 30.3% for solvent. Maximal carbachol contraction was unaffected. These results suggest a potential beneficial effect of 5-lipoxygenase inhibitors in anaphylactic reactions in man.
Poaty
V
V
Laboratoire de physiologie respiratoire, UFR Cochin Port-Royal, Paris, France.
Riquet
M
M
LeGall
G
G
Frossard
N
N
D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D016084:Bronchoconstriction / Q000187:drug effects*; D006801:Humans; D006918:Hydroxyurea / Q000031:analogs & derivatives / Q000494:pharmacology; D007073:Immunoglobulin E / Q000276:immunology*; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D015289:Leukotrienes / Q000502:physiology; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D011738:Pyrilamine / Q000494:pharmacology; D002722:Chlorobenzenes
D000888:Antibodies, Anti-Idiotypic; D006918:Hydroxyurea; D007073:Immunoglobulin E; D007211:Indoles; D015289:Leukotrienes; D016859:Lipoxygenase Inhibitors; D011738:Pyrilamine; C026408:anti-IgE antibodies; C060893:MK-886; D002722:Chlorobenzenes; C063449:zileuton