Effects of serum and insulin-like growth factors on human neuroblastoma cell growth.
Insulin-like growth factors (IGF-I and IGF-II) are mitogenic polypeptides expressed in both developing and adult tissues. To examine the effects of IGFs on neuronal growth, we used SH-SY5Y neuroblastoma cells as an in vitro model of nervous system development. In the current study, we found that either IGF-I (0.1 to 10 nM), insulin (0.1 to 5 micrograms/ml) or calf serum (0.1 to 3%) increased SH-SY5Y proliferation over a 3 day period in a dose dependent manner. In each case, treatment with anti-IGF-I receptor antibodies blocked cell proliferation. IGF-II mRNA levels correlated with SH-SY5Y cell density; subconfluent cells expressed high levels of IGF-II mRNA while low levels of IGF-II mRNA were present in confluent cells. Similarly, serum deprivation increased IGF-I receptor mRNA by 4-fold. Collectively, these results support the concept that an IGF/IGF-I receptor system at least partially mediates SH-SY5Y cell proliferation and suggests the importance of IGFs in regulating neuronal growth.
Meghani
M A
MA
Department of Neurology, University of Michigan, Ann Arbor.
Martin
D M
DM
Singleton
J R
JR
Feldman
E L
EL
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001769:Blood; D015152:Blotting, Northern; D002417:Cattle; D002460:Cell Line; D016895:Culture Media, Serum-Free; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis / Q000494:pharmacology*; D007700:Kinetics; D009447:Neuroblastoma; D012334:RNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology; D017527:Receptor, IGF Type 2 / Q000096:biosynthesis; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects
D000906:Antibodies; D016895:Culture Media, Serum-Free; D018076:DNA, Complementary; D007328:Insulin; D012334:RNA, Neoplasm; D017527:Receptor, IGF Type 2; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D017526:Receptor, IGF Type 1