Analysis of biogenic amines in plasma of hypertensive patients and a control group.
Procedures were developed for the determination of 17 circulating amines using gas chromatography-negative ion chemical ionisation mass spectrometry. The amines were quantified against their appropriate deuterated isotopomers. The mean concentrations and ranges of catecholamines and trace amines were high compared with previous studies. In comparison with nonhypertensives, plasma from hypertensives had higher concentrations of the following amines: noradrenaline (t = 4.0%); normetanephrine (t = 6.1%) and metanephrine (t = 1.9%). There were no significant differences between 5HT levels in plasma from hypertensives and controls. The following trace amines could be detected in variable amounts in plasma: p-tyramine, m-tyramine, p-octopamine, m-octopamine, p-synephrine, m-synephrine, and salsolinol. The trace amines melatonin, N-acetyl 5HT, tryptamine, 6-hydroxymelatonin and 5-methoxytryptamine could not be detected in plasma with limits of detection lying in the range 20-100 pg ml-1.
Andrew
R
R
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, U.K.
Best
S A
SA
Watson
D G
DG
Midgley
J M
JM
Reid
J L
JL
Squire
I B
IB
D001679:Biogenic Amines / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006973:Hypertension / Q000097:blood*; D007546:Isoquinolines / Q000097:blood; D008676:Metanephrine / Q000097:blood; D009638:Norepinephrine / Q000097:blood; D009647:Normetanephrine / Q000097:blood; D009655:Octopamine / Q000097:blood; D013578:Synephrine / Q000097:blood; D014439:Tyramine / Q000097:blood
D001679:Biogenic Amines; D007546:Isoquinolines; D009647:Normetanephrine; D009655:Octopamine; D008676:Metanephrine; C036617:salsolinol; D013578:Synephrine; D009638:Norepinephrine; D014439:Tyramine