Radiosensitivity of CD45RO+ memory and CD45RO- naive T cells in culture.
Radiosensitivities of various human T-cell subsets were investigated by a proliferation assay and by a single-cell gel electrophoresis assay. Each T-cell subset was purified using a cell sorter and was induced to proliferate by ionomycin and interleukin 2. Unsorted T cells showed biphasic dose-survival curves, indicating the heterogeneity of T cells in terms of radiosensitivity. Purified CD4+ helper and CD8+ killer T cells showed similar biphasic dose-survival curves. Hence both T-cell subsets were composed of cells of different radiosensitivity. The T-cell subsets belonging to different activation stages such as CD45RO+ memory and CD45RO- naive T cells had different dose-survival curves. The former was more radiosensitive than the latter. The high radiosensitivity of CD45RO+ cells was also demonstrated by single-cell gel electrophoresis after irradiation. This is the first demonstration that a particular cell surface marker on T cells is correlated with greater radiosensitivity.
Uzawa
A
A
Division of Radiation Health, National Institute of Radiological Sciences, Chiba, Japan.
Suzuki
G
G
Nakata
Y
Y
Akashi
M
M
Ohyama
H
H
Akanuma
A
A
D015703:Antigens, CD / Q000032:analysis*; D002470:Cell Survival / Q000276:immunology / Q000528:radiation effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000528:radiation effects*; D013601:T-Lymphocytes / Q000276:immunology / Q000528:radiation effects*; D013936:Thymidine / Q000378:metabolism
D015703:Antigens, CD; D004247:DNA; D017493:Leukocyte Common Antigens; D013936:Thymidine