Excitation-induced Ca2+ dynamics in sympathetic neurons measured with conventional epifluorescence and confocal UV laser-scanning microscopes.
A depolarization-induced rise in intracellular Ca2+ in cultured bullfrog sympathetic ganglion cells depended non-linearly on Ca2+ influx and membrane depolarization, spread inwardly faster than that without Ca(2+)-induced Ca2+ release (CICR), and was blocked by dantrolene and ryanodine. Ca2+ entry and a graded activation of CICR thus induce the "Ca2+ transient."
Hua
S Y
SY
Department of Physiology, Saga Medical School, Japan.
Kuba
K
K
Nohmi
M
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004594:Electrophysiology; D016257:Fura-2; D005728:Ganglia, Sympathetic / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D007834:Lasers; D008856:Microscopy, Fluorescence; D008860:Microscopy, Ultraviolet; D009474:Neurons / Q000378:metabolism*; D011892:Rana catesbeiana
D002118:Calcium; D016257:Fura-2