title: Cloning and expression of the EP2 subtype of human receptors for prostaglandin E2.
abstract: Prostaglandin E2 (PGE2) is a potent mediator in many human tissues, that is recognized by three distinct subtypes of receptors, designated EP1, EP2 and EP3. A cDNA from a human lung library encodes a 53 kDa protein of 88% homology with the mouse EP2 receptor. Human EP2 receptors in COS-7 cell transfectants bound [3H]-PGE2 with a mean Kd of 2.2 nM and native specificity, and transduced increases in the intra-cellular concentration of cyclic AMP, but not of Ca++. That most EP2 receptor mRNA is in lung, kidney, intestinal, glandular and immune tissues, is consistent with functional responses.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143-0724.
authors.forename: S
authors.initials: S
authors.lastname: An
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Xia
authors.forename: E J
authors.initials: EJ
authors.lastname: Goetzl
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D010716:Phosphatidylinositols / Q000378:metabolism; D018078:Receptors, Prostaglandin E / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D010716:Phosphatidylinositols; D018078:Receptors, Prostaglandin E; D011994:Recombinant Proteins; D000242:Cyclic AMP; D015232:Dinoprostone; D002118:Calcium