title: Comparison of monoclonal antibodies AUA1 and BER EP4 with anti-CEA for detecting carcinoma cells in serous effusions and distinguishing them from mesothelial cells.
abstract: Commercially available monoclonal antibodies AUA1, BER EP4 and carcinoembryonic antigen (CEA) were applied to cell blocks from 95 serous effusions. AUA1 and BER EP4 were reactive with 89% of effusions known to contain carcinoma cells, and anti-CEA with 71%. They also reacted with cells in two effusions from patients with malignant disease which were regarded as negative on conventional cytological examination of Papanicolaou-stained smears. They were negative in all but one of the benign effusions. Using all three antibodies, 95% of effusions containing carcinoma cells were detected. Use of these antibodies could improve the cytological diagnosis of serous effusions.
authors.affiliation: Department of Histopathology, Beaumont Hospital, Dublin, Ireland.
authors.forename: R J
authors.initials: RJ
authors.lastname: Robinson
authors.forename: D
authors.initials: D
authors.lastname: Royston
mesh_terms: D000911:Antibodies, Monoclonal; D001202:Ascitic Fluid / Q000737:chemistry / Q000473:pathology*; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002277:Carcinoma / Q000737:chemistry / Q000473:pathology*; D004847:Epithelial Cells; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen