Serum-free conditioned medium of human diploid fibroblast cells contains an activity that stimulates prostacyclin production by cultured bovine aortic endothelial cells.
Serum-free culture medium conditioned by human diploid fibroblast cells stimulated prostacyclin production by cultured bovine aortic endothelial cells. Gel filtration chromatography using Sephacryl S-100HR revealed at least three peaks of prostacyclin-stimulating activity. This factor was relatively heat-stable, acid-labile, trypsin-sensitive and bound to heparin Sepharose CL-6B. This factor was completely inhibited by indomethacin.
Yamauchi
T
T
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Umeda
F
F
Masakado
M
M
Ono
Y
Y
Nawata
H
H
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D014357:Trypsin / Q000378:metabolism
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D014357:Trypsin