Subcloning of HIV-2 nef genes in E. coli and immunological reactivity of expressed fusion proteins.
The nef gene located in the 3' region of the HIV-2 genome encodes an N-terminally myristylated protein of 27-35 kD, likely to be involved in the regulation of viral transcription. The nef genes of HIV-2 isolates GH-1, ROD, ST, BEN, and D194.17 were inserted into E. coli pEX vectors and expression of Nef beta-galactosidase fusion proteins was detected in stained gels. All fusion proteins specifically reacted with a rabbit serum raised against bacterially expressed Nef from HIV-2D194.17. Sera from monkeys inoculated with HIV-2BEN or SIVMAC251 recognized the Nef proteins of only certain HIV-2 isolates. No cross-reactivity of these sera with HIV-1 Nef and of a rabbit anti-HIV-1-Nef serum with the described HIV-2 Nef fusion proteins was observed.
Ulrich
R
R
Institut für Medizinische Virologie der Medizinischen Fakultät, Charité, Humboldt-Universität, Berlin, Germany.
Siakkou
H
H
Mayer
J
J
Kienzle
N
N
Müller-Lantzsch
N
N
Krüger
D H
DH
D000818:Animals; D000914:Antibodies, Viral; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015693:Gene Products, nef / Q000235:genetics* / Q000276:immunology*; D015754:Genes, nef; D015498:HIV-2 / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007120:Immunochemistry; D008969:Molecular Sequence Data; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D054311:nef Gene Products, Human Immunodeficiency Virus
D000914:Antibodies, Viral; D004279:DNA, Viral; D015693:Gene Products, nef; D011993:Recombinant Fusion Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus