Adenoviral vector as a gene delivery system into cultured rat neuronal and glial cells.
Previous studies have demonstrated that a defective recombinant adenovirus can infect a wide range of postmitotic and slowly proliferating cell types such as hepatocytes, myotubes, pneumocytes and intestinal cells (Stratford-Perricaudet et al., Hum. Gene Ther., 1, 241-256, 1990; Quantin et al., Proc. Natl. Acad. Sci. USA, 89, 2581-2584, 1992; Jaffe et al., Nature Genetics, 1, 372-378, 1992). We have used a defective recombinant adenovirus, Ad.RSV beta gal, containing the Escherichia coli beta-galactosidase gene targeted to the nucleus under the transcriptional control of the Rous sarcoma virus long terminal repeat promoter (Stratford-Perricaudet et al., J. Clin. Invest., 90, 626-630, 1992) to infect non-dividing neural cells in primary culture. We show that 80-100% of neuronal and astroglial cells infected with a viral titre lower than 10(9) p.f.u./ml express beta-galactosidase for at least 1 month without cell damage. These results demonstrate the potential usefulness of recombinant adenovirus infection for the analysis of brain-specific gene regulation and for the transfer of genes into neural cells before their transplantation into the brain.
Caillaud
C
C
U129 INSERM, Institut Cochin de Génétique Moléculaire (ICGM), Paris, France.
Akli
S
S
Vigne
E
E
Koulakoff
A
A
Perricaudet
M
M
Poenaru
L
L
Kahn
A
A
Berwald-Netter
Y
Y
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001921:Brain / Q000502:physiology*; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D051379:Mice; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D011401:Promoter Regions, Genetic; D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection; D001616:beta-Galactosidase / Q000032:analysis / Q000096:biosynthesis*
D011994:Recombinant Proteins; D001616:beta-Galactosidase