title: Metabolism of xenobiotics by Beauveria bassiana.
abstract: 1. Diazepam, warfarin and testosterone were metabolized by whole resting cells of the fungus Beauveria bassiana IMI 12939 via oxidative reactions such as hydroxylation and N-demethylation. 2. Metabolism of each substrate was inhibited by the cytochrome P450 inhibitors SKF-525A and metyrapone, consistent with the involvement of this enzyme system in the metabolism of these drugs by B. bassiana. 3. Substrate concentration-dependent inhibition was observed during diazepam metabolism by this organism, as has been observed in some mammalian systems. 4. Unlike most mammalian P450 systems, the warfarin-metabolizing activity of B. bassiana could not be induced by growing the organism in the presence of phenobarbitone, beta-naphthoflavone, 3-methylcholanthrene, 1-benzylimidazole or warfarin. 5. Overall findings indicate that B. bassiana possesses an oxidative metabolizing system capable of producing metabolites found in mammalian systems.
authors.affiliation: Cranfield Biotechnology Centre, Cranfield Institute of Technology, Bedfordshire, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Griffiths
authors.forename: D E
authors.initials: DE
authors.lastname: Brown
authors.forename: S G
authors.initials: SG
authors.lastname: Jezequel
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003975:Diazepam / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D003904:Mitosporic Fungi / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*; D014859:Warfarin / Q000378:metabolism*
chemical_list: D013739:Testosterone; D014859:Warfarin; D003577:Cytochrome P-450 Enzyme System; D003975:Diazepam