Isolation and cloning of the gene from Escherichia coli encoding glutamate decarboxylase.
We have isolated and cloned a 4.5-kb fragment containing the structural gene of glutamate decarboxylase from an overproducing strain of Escherichia coli. Sequencing of this fragment is in progress. The time course of glutamate decarboxylase expression has been also investigated.
De Biase
D
D
Department of Biochemical Sciences A. Rossi Fanelli, University of Rome La Sapienza, Italy.
Simmaco
M
M
Sweeney
G
G
Barra
D
D
Bossa
F
F
John
R A
RA
D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology*; D005968:Glutamate Decarboxylase / Q000096:biosynthesis / Q000235:genetics*
D004269:DNA, Bacterial; D005968:Glutamate Decarboxylase