[Basic and clinical evaluation of EIA (pin immuno assay, PIA) kit for antithyroglobulin antibody (TGAb) and antimicrosomal (antiperoxidase) antibody (TMAb)].
Newly developed enzyme immunoassay kit (Pin Immuno Assay, PIA) for quantitative determination of serum antithyroglobulin antibody (TGAb) and antimicrosomal (-peroxidase) antibody (TMAb) was evaluated. The method utilizes the Sandwich ELISA principle with a unique micropin as solid phase coated with antigen. Reproducibilities assessed by intra- and interassay variation were less than 6.9% (CV) and 7.2% for TGAb or 4.2% and 6.0% for TMAb respectively. Changes in the first or second incubation time did not affect both TGAb and TMAb values. Upper normal limits obtained from 47 healthy subjects were 150 IU/ml for TGAb and 25 IU/ml for TMAb. Positive results in TGAb were obtained 60.0% in patients with Graves' disease and 80.0% in chronic thyroiditis and in TMAb 77.8% in Graves' disease and 66.7% in chronic thyroiditis. Patients with other autoimmune diseases such as SLE, RA were also found high incidence of positive results, 48.3% for TGAb and 65.0% for TMAb respectively. These results indicate that the nonradioisotopic assay technique for thyroid autoantibodies are useful in diagnosis of autoimmune diseases especially thyroid diseases.
Uchimura
H
H
Central Clinical Laboratory, Tokyo University School of Medicine.
Aono
Y
Y
Suzuki
K
K
Takada
Y
Y
Yamaguti
H
H
Yanai
C
C
Yoshinoya
S
S
Okubo
A
A
Mitsuhashi
T
T
Kubota
K
K
D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000175:diagnosis*; D002908:Chronic Disease; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007453:Iodide Peroxidase / Q000276:immunology*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D013959:Thyroid Diseases / Q000175:diagnosis*
D001323:Autoantibodies; D011933:Reagent Kits, Diagnostic; C006606:anti-thyroglobulin; D007453:Iodide Peroxidase