Solid-phase extraction and gas chromatographic-mass spectrometric determination after hydrolysis of 2-aminofluorene haemoglobin adducts in blood of rats.
2-Nitrofluorene is an environmental pollutant that binds covalently to haemoglobin after nitroreduction and successive N-hydroxylation. these haemoglobin adducts can be cleaved in vitro by mild base-catalysed hydrolysis. For the enrichment of arylamines from the aqueous hydrolysate, an extraction procedure with an organic solvent is widely used. Because of the formation of a thick emulsion layer between the aqueous and organic solvent layers, the extraction is laborious and inefficient. The use of Amberlite XAD2 provides a simple extraction procedure yielding a recovery of ca. 70%. Calibration curves in haemoglobin solution were prepared with a correlation coefficient of 0.998 (n = 12). The inter-day coefficient of variation amounted to 14%.
Scheepers
P T
PT
Department of Toxicology, University of Nijmegen, Netherlands.
Velders
D D
DD
Straetemans
M M
MM
Ouwerkerk
J C
JC
van Vliet
L A
LA
Bos
R P
RP
D000818:Animals; D003545:Cysteine / Q000032:analysis; D005260:Female; D005449:Fluorenes / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000032:analysis*; D006868:Hydrolysis; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents
D005449:Fluorenes; D006454:Hemoglobins; D007202:Indicators and Reagents; D012997:Solvents; C012177:2-aminofluorene; D003545:Cysteine