Constitutive interferon expression from retroviral vector.
A genomic fragment with the human beta-interferon gene was cloned into a pL3-4, a defective Moloney murine leukemia virus (M-MuLV) vector. Here we show that clones selected after viral infection of mouse NIH 3T3 cells constitutively produced 128 IU/ml of human beta-interferon. Constitutive synthesis of retroviral RNA was confirmed by dot blot hybridization of RNA isolated from two of the selected clones. Poly(I) x poly(C) and cycloheximide induction resulted in an increased RNA level, but this was not reflected in an increased production of biologically active interferon.
Knezic
Z
Z
University of Southern California, School of Medicine, Norris Cancer Center, Los Angeles 90033.
Nikcevic
G
G
Marjanovic
J
J
Glisin
V
V
Popovic
Z
Z
D016475:3T3 Cells; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D005822:Genetic Vectors; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics; D011070:Poly I-C / Q000494:pharmacology; D011072:Poly U / Q000494:pharmacology; D012330:RNA, Double-Stranded / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis
D000998:Antiviral Agents; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D011072:Poly U; D016899:Interferon-beta; C047490:poly(I).poly(c12,U); D003513:Cycloheximide; D011070:Poly I-C