Transforming growth factor-beta up-regulates the gene expression of the human platelet-activating factor receptor in monocytic and B cell lines.
Platelet-activating factor is a potent phospholipid mediator which produces a wide range of biological responses by binding to a specific, high-affinity receptor on the target cell surface. Platelet-activating factor has been shown to have a very important pro-inflammatory role while the cytokine transforming growth factor-beta, also known for its diverse and potent effects, can act as both a pro- and anti-inflammatory agent. To ascertain whether transforming growth factor-beta could modulate the expression of the human platelet-activating factor receptor, different cell types were stimulated with this cytokine and the effect determined by Northern blot analysis and Ca2+ influx studies. The results demonstrate that transforming growth factor-beta induces the expression of the platelet-activating factor receptor in monocytic and B cell lines and indicate that transforming growth factor-beta could play a role in the modulation of the inflammatory response by up-regulating the expression of the platelet-activating factor receptor.
Parent
J L
JL
Department of Pediatrics, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
Stankova
J
J
D001381:Azepines / Q000494:pharmacology; D001402:B-Lymphocytes; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D009000:Monocytes; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
D001381:Azepines; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D016212:Transforming Growth Factor beta; D014230:Triazoles; C039351:platelet activating factor receptor; D003609:Dactinomycin; D015759:Ionomycin; D003513:Cycloheximide; C063964:bepafant; D002118:Calcium