title: General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer.
abstract: The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.
authors.affiliation: Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4960.
authors.forename: Q
authors.initials: Q
authors.lastname: Sun
authors.forename: A
authors.initials: A
authors.lastname: Mayeda
authors.forename: R K
authors.initials: RK
authors.lastname: Hampson
authors.forename: A R
authors.initials: AR
authors.lastname: Krainer
authors.forename: F M
authors.initials: FM
authors.lastname: Rottman
mesh_terms: D017398:Alternative Splicing / Q000502:physiology*; D000818:Animals; D002417:Cattle; D004742:Enhancer Elements, Genetic / Q000502:physiology*; D013006:Growth Hormone / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology*; D012332:RNA, Heterogeneous Nuclear / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D000068103:Serine-Arginine Splicing Factors
chemical_list: D009687:Nuclear Proteins; D012332:RNA, Heterogeneous Nuclear; D016601:RNA-Binding Proteins; D000068103:Serine-Arginine Splicing Factors; D013006:Growth Hormone