[Analysis of fatty acid composition of spotted fever group rickettsiae isolated in China by gas chromatography].
In present paper, fatty acid composition of seven Chinese isolates of SFG rickettsiae and six prototype strains of SFG rickettsiae were analyzed by GC-MS. Tested prototype strains of SFG rickettsiae were R. sibirica (strains 232 and 246), R. conorii (Simko), R. rickettsi (R), R. akari (Kaplan), R. australis (W58); Chinese isolates were An-84, Se-85, W-88 (human strain), MT-84, FT-84 (D. nuttalli strain), TO-85 (ova of nuttalli) and Chinese reference strain -JH-74 (D. nuttalli). They were propagated in yolk sacs of embryonated hen eggs and purified by centrifugation in a 30%-36%-42% discontinuous renografin density gradient. The fatty acid composition of selected strains of SFG rickettsiae was analyzed by gas chromatography, and then comparison being carried out by single linkage on mini-computer. Identification of the strains was performed based on the results obtained from GC-MS. Results showed that the fatty acid profiles of all the isolates from China were quantitatively similar to that of R. sibirica and quite different from other prototype strains of SFG rickettsiae.
Zhou
L
L
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Fan
M
M
Chen
J
J
Cai
H
H
Zhou
F
F
Zhu
H
H
D002681:China; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D012281:Rickettsia / Q000737:chemistry* / Q000145:classification
D005227:Fatty Acids