Soft agar cloning with antibody overlay to identify Chinese hamster ovary clones secreting recombinant products.
Gibson
M G
MG
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, WA.
Ashey
L
L
Newell
A H
AH
Bradley
C
C
D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D007136:Immunoglobulins / Q000032:analysis / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism*; D012685:Sepharose; D014162:Transfection
D007136:Immunoglobulins; D011994:Recombinant Proteins; D012685:Sepharose