title: Nitric oxide production from a macrophage cell line: interaction with autologous and allogeneic lymphocytes.
abstract: The indirect stimulation of macrophages to produce nitrite was examined by using the macrophage cell line J774.J774 spontaneously produced nitrite, when cultured at high concentration. J774 cultured in low concentration (< 10(4) cells in 100 microliters) barely produced nitrite. J774 cultured in low concentration produced a large amount of nitrite by the co-culture of nonadherent spleen cells or nonadherent peritoneal exudate cells, which were stimulated with con A, anti-CD3, or staphylococcal enterotoxin A. J774 (BALB/c derived: H-2d) cultured with either syngeneic (BALB/c) or allogeneic (B6; H-2b B10BR; H-2k) nonadherent lymphocytes, which were stimulated with conA or anti-CD3, produced nitric oxide. However, J774 produced nitric oxide by stimulation with SEA only when co-cultured with SEA-reactive T lymphocytes. Peritoneal exudate cells from mice, which did not proliferate by the stimulation of conA or anti-CD3, proliferated well by the addition of L-arginine homologue, NG-monomethyl-L-arginine. The proliferation of nonadherent peritoneal exudate cells stimulated with conA or anti-CD3 was suppressed by the addition of peritoneal macrophages. This suppression was abolished by the addition of NG-monomethyl-L-arginine.
authors.affiliation: Department of Immunology, Nagoya University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Isobe
authors.forename: I
authors.initials: I
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001202:Ascitic Fluid / Q000166:cytology; D017252:CD3 Complex / Q000276:immunology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000502:physiology*; D019323:omega-N-Methylarginine
chemical_list: D000906:Antibodies; D017252:CD3 Complex; D004768:Enterotoxins; D003208:Concanavalin A; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; C026066:enterotoxin A, Staphylococcal; D001120:Arginine