Association of the APC gene product with beta-catenin.
Mutations in the human APC gene are linked to familial adenomatous polyposis and to the progression of sporadic colorectal and gastric tumors. To gain insight into APC function, APC-associated proteins were identified by immunoprecipitation experiments. Antibodies to APC precipitated a 95-kilodalton protein that was purified and identified by sequencing as beta-catenin, a protein that binds to the cell adhesion molecule E-cadherin. An antibody specific to beta-catenin also recognized the 95-kilodalton protein in the immunoprecipitates. These results suggest that APC is involved in cell adhesion.
Rubinfeld
B
B
Onyx Pharmaceuticals, Richmond, CA 94806.
Souza
B
B
Albert
I
I
Müller
O
O
Chamberlain
S H
SH
Masiarz
F R
FR
Munemitsu
S
S
Polakis
P
P
D025601:Adenomatous Polyposis Coli Protein; D000595:Amino Acid Sequence; D000906:Antibodies; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D017491:Genes, APC; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011233:Precipitin Tests; D015534:Trans-Activators; D014407:Tumor Cells, Cultured; D051176:beta Catenin
D025601:Adenomatous Polyposis Coli Protein; D000906:Antibodies; C495270:CTNNB1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D009363:Neoplasm Proteins; D015534:Trans-Activators; D051176:beta Catenin