Expression of viral T-antigen in pathological tissues from transgenic mice carrying JC-SV40 chimeric DNAs.
Immunostaining methods were used to detect viral T-antigen and the cellular protein p53 in pathological tissues obtained from transgenic mice carrying JC-SV40 hybrid viral DNAs. A transgenic mouse carrying the SV40 regulatory region and JC virus (JCV) T-antigen-coding sequences exhibited an SV40-characteristic choroid plexus papilloma that expressed JCV T-antigen and p53. JCV-associated pathology was observed in two other mice in which the JCV regulatory signals directed SV40 T-antigen-induced adrenal neuroblastomas and brain neoplastic cells. However, these mice also exhibited an SV40-characteristic osteosarcoma and abdominal lymphoma that contained SV40 T-antigen and p53-positive cells. Contrasting thymic pathology was observed in the two types of mice where the SV40 regulatory region directed a JCV T-antigen-induced thymoma in one mouse, and the JCV regulatory region directed SV40 T-antigen-induced thymic hypoplasia in two other mice.
Ressetar
H G
HG
Laboratory of Experimental Neuropathology, NINDS, National Institutes of Health, Bethesda, MD 20892.
Prakash
O
O
Frisque
R J
RJ
Webster
H D
HD
Re
R N
RN
Stoner
G L
GL
D000310:Adrenal Gland Neoplasms / Q000473:pathology; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000276:immunology; D001932:Brain Neoplasms / Q000473:pathology; D002678:Chimera; D016545:Choroid Plexus Neoplasms / Q000473:pathology; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D016679:Genome, Viral; D007150:Immunohistochemistry; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D009447:Neuroblastoma / Q000473:pathology; D012516:Osteosarcoma / Q000473:pathology; D010212:Papilloma / Q000473:pathology; D011247:Pregnancy; D012888:Skull Neoplasms / Q000473:pathology; D013945:Thymoma / Q000473:pathology; D013953:Thymus Neoplasms / Q000473:pathology
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral