Rapid capillary gel electrophoresis of proteins.
The rapid separation of sodium dodecyl sulphate-protein complexes according to their molecular masses (M(r)) by capillary gel electrophoresis is described. Using commercial equipment, standard proteins with M(r) in the range 29,000-97,400 were resolved to the baseline in less than 2 min by utilizing a separation distance of 7 cm. A linear relationship between migration time and log M(r) was found and rapid determination of the molecular mass of light and heavy chains of human immunoglobulin G is reported. The results are compared with applications using longer separation distances, showing that rapid and efficient analysis and adequate resolution can be obtained by using short separation distances.
Lausch
R
R
Westfälische Wilhelms-Universität Münster, Institut für Biochemie, Germany.
Scheper
T
T
Reif
O W
OW
Schlösser
J
J
Fleischer
J
J
Freitag
R
R
D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D008970:Molecular Weight; D010047:Ovalbumin / Q000302:isolation & purification; D010763:Phosphorylase b / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
D007074:Immunoglobulin G; D011506:Proteins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D010763:Phosphorylase b; D002256:Carbonic Anhydrases