Novel oligosaccharides with the sialyl-Le(a) structure in human milk.
Two novel oligosaccharides with the sialyl-Le(a) structure were isolated from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography, and structurally characterized by a combination of 600-MHz 1H NMR spectroscopy and fast atom bombardment mass spectrometry. The structural studies indicated the structures of these oligosaccharides to be: [formula: see text]
Kitagawa
H
H
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.
Nakada
H
H
Fukui
S
S
Funakoshi
I
I
Kawasaki
T
T
Yamashina
I
I
Tate
S
S
Inagaki
F
F
D000911:Antibodies, Monoclonal; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000911:Antibodies, Monoclonal; D009844:Oligosaccharides; C058981:sialyl-Le(a) oligosaccharide