Cloning and characterization of a surface antigen of Eimeria tenella merozoites and expression using a recombinant vaccinia virus.
A rabbit serum raised against Eimeria tenella merozoites was used to screen a lambda gt11 cDNA library made from merozoite mRNA of E. tenella. The insert of the phage clone lambda Mz 5-7 revealed an open reading frame consisting of 945 nucleotides, encoding a 33-kDa protein. This size is consistent with the size of a protein translated in vitro from merozoite mRNA and immunoprecipitated with monospecific anti-Mzp 5-7 antibodies. A smaller protein of 24 kDa, located on the surface of the parasite, also reacted with the monospecific antiserum and is the potential processed form of the Mzp 5-7. Furthermore, a recombinant vaccinia virus expressing the Mzp 5-7 antigen was constructed and used to immunize chickens.
Binger
M H
MH
Vitamin Research, VRD/F, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Hug
D
D
Weber
G
G
Schildknecht
E
E
Hümbelin
M
M
Pasamontes
L
L
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D016786:Eimeria tenella / Q000378:metabolism*; D004926:Escherichia coli; D015723:Gene Library; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D016053:RNA, Protozoan / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D012333:RNA, Messenger; D016053:RNA, Protozoan; D011994:Recombinant Proteins