Perfusion with picroliv reverses biochemical changes induced in livers of rats toxicated with galactosamine or thioacetamide.
Perfusion of liver of rats toxicated with galactosamine or thioacetamide with a 0.02% solution of picroliv (glycoside fraction of Picrorhiza kurroa) for 30 min (1 ml/min; 6 mg/rat), significantly reversed toxicant-induced changes in the activities of several enzymes. Galactosamine induced increases in the activities of alkaline phosphatase, gamma-glutamyl transpeptidase, acid ribonuclease, acid phosphatase, succinate dehydrogenase and decreases in the activities of Na(+)-K(+)-adenosine triphosphatase (ATPase) and glucose-6-phosphatase (reversed by 40-87%). Similarly, thioacetamide-induced inhibitions of the activities of Na(+)-K(+)-ATPase, Ca(++)-ATPase, Mg(++)-ATPase, succinate dehydrogenase and elevations in the activities of alkaline phosphatase, gamma-glutamyl transpeptidase, and acid ribonuclease were also significantly reversed. A significant reversal of the toxicants-induced decrease in [14C]-leucine incorporation was also observed. These results indicate that picroliv can also reverse D-galactosamine- or thioacetamide-induced hepatic damage in rats.
Dwivedi
Y
Y
ICMR Centre for Advanced Pharmacological Research on Traditional Remedies, Central Drug Research Institute, Lucknow, India.
Rastogi
R
R
Garg
N K
NK
Dhawan
B N
BN
D000818:Animals; D002934:Cinnamates / Q000494:pharmacology*; D005688:Galactosamine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D006027:Glycosides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects*; D008297:Male; D010477:Perfusion; D051381:Rats; D013853:Thioacetamide / Q000633:toxicity*; D014641:Vanillic Acid / Q000494:pharmacology*
D002934:Cinnamates; D006027:Glycosides; D013853:Thioacetamide; C057463:kutkin; D005688:Galactosamine; D014641:Vanillic Acid