title: Transneuronal viral labelling of rat heart left ventricle controlling pathways.
abstract: Retrograde transneuronal viral labelling and immunocytochemical methods were used for revealing neuronal networks controlling the left ventricle myocardium of the rat heart. After injections of 1 microliter pseudorabies virus solution (3 x 10(6) PFU ml-1) into the left ventricle, infected orthosympathetic preganglionic cells were found in the intermediolateral cell groups of the first 6 thoracic spinal segments. Preganglionic parasympathetic neurones were seen both in the nucleus ambiguus/retro-ambiguus area and the dorsal motor vagus nucleus. Large numbers of infected projecting interneurones were found in the rostral, caudal and medial parts of the ventral medulla oblongata, the Kölliker-Fuse nucleus and catecholaminergic cell group A5 and in the paraventricular hypothalamic nucleus.
authors.forename: G J
authors.initials: GJ
authors.lastname: Ter Horst
authors.forename: A
authors.initials: A
authors.lastname: Van den Brink
authors.forename: S A
authors.initials: SA
authors.lastname: Homminga
authors.forename: R W
authors.initials: RW
authors.lastname: Hautvast
authors.forename: G
authors.initials: G
authors.lastname: Rakhorst
authors.forename: T C
authors.initials: TC
authors.lastname: Mettenleiter
authors.forename: M J
authors.initials: MJ
authors.lastname: De Jongste
authors.forename: K I
authors.initials: KI
authors.lastname: Lie
authors.forename: J
authors.initials: J
authors.lastname: Korf
mesh_terms: D000818:Animals; D001339:Autonomic Fibers, Preganglionic / Q000648:ultrastructure; D009202:Cardiomyopathies / Q000382:microbiology / Q000473:pathology* / Q000503:physiopathology; D006321:Heart / Q000294:innervation*; D006352:Heart Ventricles / Q000294:innervation; D011558:Herpesvirus 1, Suid; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000473:pathology; D009415:Nerve Net / Q000033:anatomy & histology; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000502:physiology; D011557:Pseudorabies / Q000382:microbiology / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology; D016276:Ventricular Function