title: Effect of sunscreens on UV radiation-induced enhancement of melanoma growth in mice.
abstract: BACKGROUND
  Epidemiologic evidence suggests that exposure to UV radiation plays a significant role in the development of melanoma skin cancers. As early surgical removal of the melanoma is the only effective therapy, current strategies for reducing mortality from melanoma focus on prevention of the disease. Chemical sunscreens protect mice from development of skin cancers that resemble sunlight-induced human squamous cell cancers, but there appears to be a complex relationship between UV radiation exposure and development of melanoma.
  
  
  OBJECTIVE
  We asked whether common sunscreens would protect mice against UV radiation-induced enhancement of melanoma incidence.
  
  
  METHODS
  C3H mice were exposed to 4.8 kJ/m2 UVB from FS40 sunlamps twice a week for 3 weeks. Sunscreens containing 7.5% 2-ethylhexyl-p-methoxycinnamate, 8% octyl-N-dimethyl-p-aminobenzoate, 6% benzophenone-3, or the oil-in-water vehicle alone were applied to the ears and tails of the mice 20 minutes before irradiation. At various times during and after exposure, we determined UV radiation-induced inflammation by measuring ear swelling. We also examined the ears histologically for UV radiation-induced alterations. One day after the final irradiation, 2.5 x 10(4) syngeneic K1735 melanoma cells were injected into the external ears. Mice were examined weekly for tumor growth for 5-8 weeks after tumor cell injection. Control mice were treated in the identical way except for exposure to UV radiation.
  
  
  RESULTS
  The incidence of melanomas was significantly higher in the UV-irradiated mice. All three sunscreens protected against UV radiation-induced ear swelling and clearly diminished histopathologic alterations, including sunburn cell formation, epidermal hyperplasia, and mononuclear cell infiltrate in the dermis. However, the sunscreens failed to protect against UV radiation-induced increase in melanoma incidence. The sunscreens or vehicle alone did not significantly alter tumor growth.
  
  
  CONCLUSIONS
  Protection against sunburn does not necessarily imply protection against other possible UV radiation effects, such as enhanced melanoma growth.
  
  
  CONCLUSIONS
  Sunscreen protection against UV radiation-induced inflammation may encourage prolonged exposure to UV radiation and thus may actually increase the risk of melanoma development. These findings suggest that further research on the ability of sunscreens to prevent melanoma is urgently needed.
authors.affiliation: Department of Immunology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
authors.forename: P
authors.initials: P
authors.lastname: Wolf
authors.forename: C K
authors.initials: CK
authors.lastname: Donawho
authors.forename: M L
authors.initials: ML
authors.lastname: Kripke
mesh_terms: D010129:4-Aminobenzoic Acid / Q000494:pharmacology; D000818:Animals; D001577:Benzophenones / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002934:Cinnamates / Q000494:pharmacology; D005260:Female; D007249:Inflammation / Q000473:pathology; D008545:Melanoma / Q000473:pathology* / Q000517:prevention & control*; D051379:Mice; D008809:Mice, Inbred C3H; D009381:Neoplasms, Radiation-Induced / Q000473:pathology* / Q000517:prevention & control*; D012878:Skin Neoplasms / Q000473:pathology* / Q000517:prevention & control*; D013473:Sunscreening Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D001577:Benzophenones; D002934:Cinnamates; D013473:Sunscreening Agents; C118580:octylmethoxycinnamate; C005290:oxybenzone; D010129:4-Aminobenzoic Acid