Orientation of small specimens for cryosectioning.
A simple technique is introduced to achieve symmetrically oriented frozen sections of small specimens such as young fish or frog larvae. Small samples are especially difficult to orient if they are already frozen to the chuck in a freezing microtome. Orientation of the sample in a mold filled with embedding medium prior to freezing permits sectioning as well as easy labeling and storage of the specimens. The use of a stereo microscope during orientation is optional.
Anken
R H
RH
Institute of Zoology, University of Stuttgart-Hohenheim, Germany.
Kappel
T
T
D000818:Animals; D001001:Anura; D016616:Cryoultramicrotomy / Q000379:methods*; D005399:Fishes; D016610:Tissue Embedding / Q000379:methods