title: Investigation of the mechanisms underlying the increased contraction of hypertrophied ventricular myocytes isolated from the spontaneously hypertensive rat.
abstract: OBJECTIVE
  The aim was to investigate the cellular mechanisms responsible for the increased contraction of left ventricular myocytes isolated from the spontaneously hypertensive rat (SHR).
  
  
  METHODS
  Single myocytes were isolated enzymatically from the left ventricles of SHR, Wistar-Kyoto (WKY), and Wistar rats. WKY and Wistar myocytes were used as normotensive controls. Cytoplasmic calcium was measured with Fura-2 and contraction was measured optically. Membrane potential was measured with microelectrodes and cells were voltage clamped to measure the amplitude of L-type calcium current (iCa).
  
  
  RESULTS
  Under action potential conditions, SHR myocytes had a larger calcium transient and an increased sarcoplasmic reticular calcium content compared to normotensive myocytes. There was no detectable difference in the resting cytoplasmic calcium concentration between SHR and control myocytes. SHR myocytes also had a prolonged action potential compared to normotensive cells. However, when cells were voltage clamped and short pulses of 120 ms duration were applied (a similar duration of depolarisation to the action potential), the difference in the calcium transient or contraction between SHR and normotensive myocytes was abolished. SHR myocytes had an unchanged amplitude of ICa in comparison to control myocytes, and there was no detectable difference in the myofilament response to calcium between SHR and control myocytes.
  
  
  CONCLUSIONS
  (1) Hypertrophied SHR myocytes stimulated with action potentials had an increased calcium transient compared to normotensive cells. The greater calcium transient in the SHR cells is likely to be a major factor responsible for their increased contraction. (2) SHR myocytes had a prolonged action potential in comparison to normotensive cells. (3) The amplitude of ICa and myofilament response to calcium were unchanged in SHR myocytes, suggesting that these factors do not play a role in the increased contraction of these cells. (4) Since the difference between SHR and control cells was abolished by voltage clamping the cells to prevent the difference of action potential, it is unlikely that an alteration of intrinsic mechanisms in SHR myocytes is responsible for their increased contraction. Rather, it suggests that the prolonged action potential of SHR myocytes plays a important role in causing their increased calcium transient and contraction. Our results indicate that the prolonged action potential in SHR cells results in an increased calcium content of the sarcoplasmic reticulum, which leads to a greater sarcoplasmic reticular calcium release upon stimulation and an increased contraction.
authors.affiliation: Department of Physiology, School of Medical Sciences, University of Bristol, University Walk, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Brooksby
authors.forename: A J
authors.initials: AJ
authors.lastname: Levi
authors.forename: J V
authors.initials: JV
authors.lastname: Jones
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004558:Electric Stimulation; D006973:Hypertension / Q000378:metabolism / Q000473:pathology; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017208:Rats, Wistar
chemical_list: D000252:Calcium-Transporting ATPases; D002118:Calcium