Immediate metabolic availability of dietary fat in combination with carbohydrate.
We tested the hypothesis that when fat is ingested in combination with carbohydrate, direct release of fatty acids into the plasma may occur. Eight normal subjects ingested two meals: high-fat (80 g fat, 80 g carbohydrate, and 18 g protein) and low-fat (< 1 g fat, otherwise closely matched). Over the subsequent 6 h, net fat oxidation was greater after the high-fat meal (20.7 vs 10.6 g, P < 0.01). Plasma nonesterified fatty acid (NEFA) concentrations were markedly suppressed after the low-fat meal, but relatively maintained after the high-fat meal (P < 0.01). The profile of plasma NEFAs changed after the high-fat meal, consistent with entry of meal-derived fatty acids into the NEFA pool. We suggest that after ingestion of combinations of carbohydrate and fat, the action of lipoprotein lipase on chylomicron-triacylglycerol leads to direct release of fatty acids into the plasma and increased fat oxidation.
Griffiths
A J
AJ
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, University of Oxford, UK.
Humphreys
S M
SM
Clark
M L
ML
Fielding
B A
BA
Frayn
K N
KN
D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D002153:Calorimetry, Indirect; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004041:Dietary Fats / Q000097:blood / Q000378:metabolism*; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D010101:Oxygen Consumption; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014280:Triglycerides