title: Sequence conservation in field and experimental isolates of Borna disease virus.
abstract: Coding and noncoding sequences were analyzed from field and experimental isolates of Borna disease virus. For a 24-kDa protein, maximum divergence was 1.5% at the predicted amino acid level and 3.1% at the nucleotide level. For a 40-kDa protein, maximum divergence was 1.1% at the predicted amino acid level and 4.1% at the nucleotide level. The highest variability in sequence (10%) was found in a 40-nucleotide stretch of genomic RNA between coding sequences for the 40- and 24-kDa proteins. The degree of sequence conservation in these isolates, passaged in various host species in vivo and in vitro over a period of 64 years, is unusual for negative-strand RNA viruses.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Irvine 92717.
authors.forename: P A
authors.initials: PA
authors.lastname: Schneider
authors.forename: T
authors.initials: T
authors.lastname: Briese
authors.forename: W
authors.initials: W
authors.lastname: Zimmermann
authors.forename: H
authors.initials: H
authors.lastname: Ludwig
authors.forename: W I
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D000818:Animals; D001483:Base Sequence; D001890:Borna Disease / Q000382:microbiology; D001891:Borna disease virus / Q000235:genetics*; D001921:Brain / Q000382:microbiology; D003001:Cloning, Molecular; D017124:Conserved Sequence; D014644:Genetic Variation; D005858:Germany; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses / Q000382:microbiology; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012692:Serial Passage; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; C084757:p24 protein, Borna disease virus; C077347:p40 protein, Borna disease virus