The cellular U-particle, whose synthesis is induced by mengovirus infection, is homologous to apoferritin.
Mengo virus infection of mouse L-cells results in induction of the synthesis of a cellular protein-containing particle, 12 nm in diameter, which was designated U (Boege et al. (1987) Virology 159, 358-367). We have purified the U-particle from virus-infected cells by a series of chromatographic steps and found it to be composed of two polypeptide species (MW 23,000 and 25,000), present in a ratio of approximately 7:3. Neither of these polypeptides is measurably glycosylated or phosphorylated and the U-particle contains no detectable nucleic acid. Several amino acid sequences obtained from CNBr fragments of the U-polypeptides identified them as the H- and L-chains of mouse apoferritin. This finding was supported by immunoblotting and electron microscopy. In terms of function, the U-particle/apoferritin effectively inhibits the translation of mRNAs in reticulocyte lysates. These experiments indicate that apoferritin may perform important functions in eukaryotic cells in addition to iron storage. Finally, we propose mechanisms to explain how Mengo virus infection could specifically induce the synthesis of apoferritin and how increasing amounts of cytoplasmic apoferritin could facilitate virus replication.
Mulvey
M R
MR
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Fang
H
H
Holmes
C F
CF
Scraba
D G
DG
D000595:Amino Acid Sequence; D000818:Animals; D001052:Apoferritins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D007181:Inclusion Bodies, Viral / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D007739:L Cells; D008574:Mengovirus / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D001052:Apoferritins