Evans blue extravasation in rat dental pulp and oral tissues induced by electrical stimulation of the inferior alveolar nerve.
Whether increased extravasation of plasma protein may occur in the rat incisor pulp as a result of antidromic stimulation of afferent nerves was investigated, and this preinflammatory reaction compared with that in adjacent soft tissues. In anaesthetized rats, the inferior alveolar nerve was exposed and stimulated electrically (10-15 V, 2 ms, 10 Hz for 30 s or 5 min). Blood flow changes in the lower lip and incisor pulp were recorded by laser Doppler flowmetry. Increased vascular permeability in the lip, gingiva and pulp was indirectly determined by means of the Evans blue dye method and spectrophotometric analysis. Stimulation of the inferior alveolar nerve for 30 s, in the presence of the alpha-adrenergic blocker phenoxybenzamine (3 mg/kg), increased blood flow in the lip by 172 +/- 16% and in the pulp by 38 +/- 5% as compared to basal blood flow. Intravenous (i.v.) administration of atropine (1 mg/kg), chlorisondamine (3 mg/kg), timolol (150 micrograms/kg), cimetidine plus mepyramine (3 mg/kg of each), methysergide (1 mg/kg) and diclofenac sodium (3 mg/kg) was without effect on this response. Acute pretreatment with capsaicin (1-3 mg/kg, i.v.), however, abolished the vasodilation in the pulp and reduced that in the lip by 58% (p < 0.05). In untreated animals, stimulation of the inferior alveolar nerve for 5 min increased the Evans blue content in the ipsilateral lip by 164% (p < 0.001), gingiva by 55% (p < 0.01) and pulp by 67% (p < 0.01). Pretreatment (i.v.) with a combination of cimetidine and mepyramine counteracted the dye extravasation only in the gingiva.(ABSTRACT TRUNCATED AT 250 WORDS)
Kerezoudis
N P
NP
Department of Pharmacology, Karolinska Institute, Stockholm, Sweden.
Olgart
L
L
Edwall
L
L
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology; D002714:Chlorisondamine / Q000494:pharmacology; D003782:Dental Pulp / Q000098:blood supply / Q000473:pathology*; D004008:Diclofenac / Q000494:pharmacology; D004558:Electric Stimulation; D005070:Evans Blue; D005119:Extravasation of Diagnostic and Therapeutic Materials / Q000473:pathology* / Q000503:physiopathology; D005881:Gingiva / Q000098:blood supply / Q000473:pathology*; D008046:Lip / Q000098:blood supply / Q000473:pathology*; D008297:Male; D008340:Mandibular Nerve / Q000502:physiology*; D008784:Methysergide / Q000494:pharmacology; D010643:Phenoxybenzamine / Q000494:pharmacology; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D013999:Timolol / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology
D001798:Blood Proteins; D010643:Phenoxybenzamine; D004008:Diclofenac; D005070:Evans Blue; D013999:Timolol; D011738:Pyrilamine; D002714:Chlorisondamine; D002211:Capsaicin; D008784:Methysergide