HPLC analysis of optical isomers of leucovorin and methotrexate using achiral-chiral system.
Chiral high performance liquid chromatography was used for the enantiometric separation of leucovorin. The optimal separation conditions recommended after optimizing the mobile phase composition, flow rate, and temperature are described. Achiral reversed-phase chromatographic method was applied for the simultaneous separation of methotrexate and leucovorin in clinical samples. Column-switching system including achiral short pre-column and chiral analytical column based on immobilized bovine serum albumin were used for simultaneous determination of leucovorin and methotrexate patients treated at the National Cancer Institute.
Brandsteterová
E
E
Department of Analytical Chemistry, Slovak Technical University, Bratislava.
Marcincinová
K
K
Lehotay
J
J
Zbojová
A
A
Halko
J
J
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D002955:Leucovorin / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D008727:Methotrexate / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D013237:Stereoisomerism
D007202:Indicators and Reagents; D002955:Leucovorin; D008727:Methotrexate