Protective immune response to Mycoplasma gallisepticum demonstrated in respiratory-tract washings from M. gallisepticum-infected chickens.
Chickens inoculated with Mycoplasma gallisepticum (MG) produced IgA, IgM, and IgG detectable in washings from the upper respiratory tract (URTW; nasal sinuses and turbinates) and lower respiratory tract (LRTW; trachea, lungs, and air sacs). URTW and LRTW from infected chickens had significant protective effects in a MG-inoculated tracheal-ring-organ-culture system. Protective effects in vitro correlated positively with total MG-specific immunoglobulin titer, but not IgA titer, as determined by enzyme-linked immunosorbent assay. URTW and LRTW from infected chickens inhibited attachment of MG to tracheal-ring-organ cultures in a dose-dependent manner. This suggests that chickens produce a protective immune response to MG that locates in the respiratory tract and that attachment inhibition may be responsible for this protective effect.
Avakian
A P
AP
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Ley
D H
DH
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001422:Bacterial Adhesion / Q000276:immunology; D002645:Chickens; D007111:Immunity, Cellular; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D009174:Mycoplasma / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D009924:Organ Culture Techniques; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology; D012137:Respiratory System / Q000276:immunology / Q000382:microbiology; D014132:Trachea / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M