Isoflurane inhibits insulin secretion from isolated rat pancreatic islets of Langerhans.
We have investigated the effects of isoflurane on insulin secretion in vitro from rat isolated islets of Langerhans and found a significant, dose-related and reversible inhibition of insulin secretion. Isoflurane 2% decreased insulin secretion stimulated by glucose 20 mmol litre-1 to basal, nonstimulated values. In other studies to identify the stage in the stimulus secretion pathway for insulin at which the anaesthetic may exert an inhibitory action, we have stimulated insulin release using glyceraldehyde and a phorbol ester. Insulin secretion induced by these secretagogues was also blocked by isoflurane. This suggests that the inhibitory effect of the anaesthetic agent may be at a site distal to stimulation of insulin secretion by glyceraldehyde and phorbol esters.
Desborough
J P
JP
Department of Anaesthesia, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Jones
P M
PM
Persaud
S J
SJ
Landon
M J
MJ
Howell
S L
SL
D000769:Anesthesia, Inhalation; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005985:Glyceraldehyde / Q000037:antagonists & inhibitors; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007530:Isoflurane / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013755:Tetradecanoylphorbol Acetate
D007328:Insulin; D005985:Glyceraldehyde; D007530:Isoflurane; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate