Autocrine regulation of the production of the gaseous messenger nitric oxide in a glioblastoma cell line.
We used a glioblastoma multiform (GBM) cell line to study the mechanism of cellular regulation of nitric oxide (NO) production. Our experiments indicate a confluent monolayer of GBM cells to release NO as measured through its oxidized NO2 form which gradually accumulates and reaches a peak by 7 to 10 days of culture. the addition of the L-arginine analogs L-NG-monomethyl-L-arginine and L-N omega-nitro-L-arginine and dexamethasone to the GBM cultures caused a substantial inhibition of NO production. The addition of monoclonal antibodies against IL-1 and TNF alpha to the cultures resulted in an inhibition of NO production, whereas the addition of anti-TGF beta monoclonal antibodies resulted in an increase in NO production. These findings suggest the presence of an autocrine regulatory mechanism for NO production in some tumor cell lines.
Haregewoin
A
A
Brain Tumor Center, Brigham and Women's Hospital, Boston, Massachusetts.
Alexander
E
E
Black
P M
PM
Loeffler
J S
JS
D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D016207:Cytokines / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology; D005909:Glioblastoma / Q000378:metabolism*; D066298:In Vitro Techniques; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D019323:omega-N-Methylarginine
D016207:Cytokines; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D003907:Dexamethasone; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester