Differential expression of tomato (Lycopersicon esculentum L.) genes encoding shikimate pathway isoenzymes. II. Chorismate synthase.
In tomato (Lycopersicon esculentum L. cv. UC82b) two distinct genes (designated LeCS1 and LeCS2) code for chorismate synthase. The corresponding cDNAs have been isolated and characterized. The deduced amino acid sequences are 88% identical. Both genes encode chorismate synthases with putative plastid-specific N-terminal transit peptides. The two genes are predominantly expressed in flowers and roots and, to a lesser extent, in stems, leaves, and cotyledons, but the steady-state levels of LeCS1-specific transcripts are consistently higher than those of the LeCS2-specific transcripts.
Görlach
J
J
Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich.
Schmid
J
J
Amrhein
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D017343:Genes, Plant; D007527:Isoenzymes / Q000235:genetics; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D019761:Phosphorus-Oxygen Lyases; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D018087:Plastids / Q000201:enzymology; D011061:Poly A / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012765:Shikimic Acid / Q000378:metabolism
D015342:DNA Probes; D018076:DNA, Complementary; D007527:Isoenzymes; D010940:Plant Proteins; D012333:RNA, Messenger; D011061:Poly A; D012765:Shikimic Acid; D008190:Lyases; C019895:chorismate synthase; D019761:Phosphorus-Oxygen Lyases