Loss of heterozygosity detected in formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using a microsatellite located within the deleted in colorectal carcinoma gene.
We determined loss of heterozygosity from formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using microsatellite polymorphism. The polymorphism was assayed based on DNA amplification by the polymerase chain reaction (PCR). The PCR-analyzed microsatellite method was applied to assay degraded DNA extracted from paraffin-embedded blocks with adenocarcinoma of colon. The DNA from 26 tumors as well as their corresponding normal tissue samples were successfully amplified using a dinucleotide microsatellite located within an intron of the deleted in colorectal carcinoma gene. Allele losses on this marker were detected in 33% of informative colorectal carcinomas. This study demonstrates that microsatellites provide a powerful set of DNA markers for loss of heterozygosity on archival specimens.
Huang
T H
TH
Department of Pathology, Ellis Fischel Cancer Center, Columbia, Missouri.
Quesenberry
J T
JT
Martin
M B
MB
Loy
T S
TS
Diaz-Arias
A A
AA
D000230:Adenocarcinoma / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D005557:Formaldehyde; D017353:Gene Deletion; D016163:Genes, DCC; D006579:Heterozygote; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics
D004276:DNA, Satellite; D005557:Formaldehyde