Uptake of fluorescein isothiocyanate (FITC)-fractionated heparin by rat parenchymal hepatocytes in primary culture.
The distribution of fractionated heparin in a primary culture of rat parenchymal hepatocytes was investigated optically using the fluorescence labelled drug and confocal imaging system with an inverted fluorescence microscope. The cell-associated fluorescein isothiocyanate (FITC)-fractionated heparin was observed to increase in conjunction with incubation time and also to localize, suggesting an internalization to cell organella with the exception of the nuclei.
Watanabe
J
J
Department of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Muranishi
H
H
Haba
M
M
Yuasa
H
H
D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D005591:Chemical Fractionation; D016650:Fluorescein-5-isothiocyanate; D006493:Heparin / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D006493:Heparin; D016650:Fluorescein-5-isothiocyanate