Inhibition by heparin of thrombin-catalyzed activation of the factor VIII-von Willebrand factor complex.
The activation of factor VIII (fVIII) by thrombin is associated with heavy chain cleavages at Arg372 and Arg740 and light chain cleavage at Arg1689. In a defined, plasma-free assay of fVIII activation and at physiological ionic strength and pH, heparin inhibited the rate of activation of either human or porcine fVIII by thrombin in either the presence or absence of von Willebrand factor (vWf). The inhibitory effect of heparin was associated with inhibition of all three thrombin-catalyzed bond cleavages. At plasma concentrations of fVIII (approximately 1 nM) and vWf (approximately 35 nM), the rate of fVIII activation was inhibited by 50% at approximately 0.1 unit/ml heparin, which is below the normal range of heparin concentrations in plasma during therapeutic anticoagulation (0.2-0.7 unit/ml). We propose that, in addition to catalyzing the inhibition of thrombin and other intrinsic pathway coagulation proteases by antithrombin, heparin functions as an anticoagulant by direct inhibition of the activation of the fVIII-vWf complex by thrombin.
Barrow
R T
RT
Department of Medicine, Emory University, Atlanta, Georgia 30322.
Healey
J F
JF
Lollar
P
P
D000818:Animals; D002384:Catalysis; D005169:Factor VIII / Q000378:metabolism*; D005340:Fibrinogen / Q000494:pharmacology; D006493:Heparin / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D012685:Sepharose / Q000378:metabolism; D013552:Swine; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014841:von Willebrand Factor / Q000378:metabolism*
D014841:von Willebrand Factor; D005169:Factor VIII; D005340:Fibrinogen; D006493:Heparin; D012685:Sepharose; D013917:Thrombin