[A spin-probe study of the structural change in human blood lipoproteins under the action of sodium hypochlorite].
The spin-probe technique was used to study structural changes in the surface proteolipid layer of human blood low-density lipoproteins (LDL) peroxidized to different degree by sodium hypochlorite (NaOCl). The paramagnetic analogs of stearic acid were employed as spin labels that contained nitroxide groups localized at different distances from the LDL surface. In addition, a positively charged spin label was used which was distributed at the lipid-water interface, in the area of polar phospholipid groups. NaOCl was shown to induce the lipid peroxidation of LDL. It diminished the mobility of polar "heads" as well as of acyl phospholipid chains up to 1.7 nm from the LDL particle surface and increased the polarity of lipid phase in this region. In the deeper layers (2.0-2.2 nm) the structural alterations in LDL were registered only at NaOCl concentration more than 1 mM.
Panasenko
O M
OM
Evgina
S A
SA
Sergienko
V I
VI
D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood* / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013113:Spin Labels; D013499:Surface Properties; D014461:Ultracentrifugation
D008077:Lipoproteins, LDL; D013113:Spin Labels; D012973:Sodium Hypochlorite
Issledovanie metodom spinovykh zondov izmeneniia struktury lipoproteinov krovi cheloveka pod deĭstviem gipokhlorita natriia.