[Interrelationship of microsomal and mitochondrial systems for hydroxylating steroids from cattle adrenal cortex: effect of highly-purified components of the mitochondrial monoxygenase system on hydroxylation of steroids by microsomes].
The possibility of functioning of microsomal steroid hydroxylases in the adrenal cortex in the presence of the electron-transporting proteins in mitochondrial monooxygenase systems has been studied. It was found that adrenodoxin reductase stimulates both the 21- and 17 alpha-steroid hydroxylation activities in microsomes of the adrenal cortex with the same efficiency as does NADPH-dependent cytochrome P-450 reductase. Using cytochrome b5 and its specific antibody, an attempt has been made to investigate possible mechanisms of the stimulating effect of adrenodoxin reductase.
Guzov
V M
VM
Zel'ko
I N
IN
Chernogolov
A A
AA
Usanov
S A
SA
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D015786:Cytochromes b5 / Q000378:metabolism; D004579:Electron Transport; D006900:Hydroxylation; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis / Q000378:metabolism*
D013256:Steroids; D015786:Cytochromes b5; D006899:Mixed Function Oxygenases; D013250:Steroid Hydroxylases; D009251:NADPH-Ferrihemoprotein Reductase
Bzaimootnosheniia mikrosomal'nykh i mitokhondrial'nykh sistem gidroxsilirovaniia steroidov kory nadpochechnikov byka: vliianie vysokoochishchennykh komponentov mitokhondrial'noĭ monooksigenaznoĭ sistemy na gidroksilirovanie steroidov mikrosomami.