Flow cytometric analysis of the neutralizing immune response against infectious bursal disease virus using reticuloendotheliosis virus-transformed lymphoblastoid cell lines.
Infectious bursal disease virus (IBDV) is a lymphotropic virus with cytocidal effect on B lymphocytes of the bursa of Fabricius. We investigated the susceptibility of clonal populations of reticuloendotheliosis virus-transformed chicken B lymphocytes of both spleen and bursal origin to IBDV infection. The infected cells were metabolically-labelled and the viral polypeptides were analyzed by immunoprecipitation using monoclonal antibodies (MAbs). Virus adsorption and the effects of neutralizing convalescent antisera and MAbs on virus attachment were studied using flow cytometry. The results of the study indicate firstly that the transformed B cells support virus replication and provide an efficient system for studying IBDV-lymphocyte interactions. Secondly, results obtained also showed that the most potent neutralizing antibodies may not be those involved in preventing the receptor-mediated viral attachment but rather those involved in the inhibition of downstream events such as virus penetration or uncoating.
Reddy
S K
SK
Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Quebec, Canada.
Ratcliffe
M J
MJ
Silim
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000382:microbiology*; D002060:Bursa of Fabricius; D002461:Cell Line, Transformed; D002469:Cell Separation; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005347:Fibroblasts; D005434:Flow Cytometry; D007106:Immune Sera; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000502:physiology; D009500:Neutralization Tests; D011991:Receptors, Virus / Q000378:metabolism; D001356:Reticuloendotheliosis virus; D013154:Spleen; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007106:Immune Sera; D011991:Receptors, Virus