title: Dense nonsymmetrical DNA methylation resulting from repeat-induced point mutation in Neurospora.
abstract: Cytosine methylation has been implicated in epigenetic control of gene expression in animals, plants, and fungi. It has been assumed that all methylation in eukaryotes is at symmetrical sequences such as CpG/GpC, because this can explain perpetuation of methylation states. Here the bisulfite genomic sequencing method was used to examine methylation in DNA from a Neurospora gene exposed to repeat-induced point mutation. 5-Methylcytosine was not limited to symmetrical sites and individual molecules showed different patterns and amounts of modification. The methylation extended beyond the mutated region and even beyond the edge of the duplicated segment.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403.
authors.forename: E U
authors.initials: EU
authors.lastname: Selker
authors.forename: D Y
authors.initials: DY
authors.lastname: Fritz
authors.forename: M J
authors.initials: MJ
authors.lastname: Singer
mesh_terms: D044503:5-Methylcytosine; D001483:Base Sequence; D015139:Blotting, Southern; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D008745:Methylation; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D017354:Point Mutation
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D044503:5-Methylcytosine; D003596:Cytosine; D005969:Glutamate Dehydrogenase; C061421:glutamate dehydrogenase (NAD(P)+); D004262:DNA Restriction Enzymes