title: Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection.
abstract: A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). limits of detection were estimated to be 1200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (microgram/ml) were approximately the same as in sciatic nerve (microgram/g).
authors.affiliation: Research Triangle Institute, Research Triangle Park, NC 27709.
authors.forename: J H
authors.initials: JH
authors.lastname: Raymer
authors.forename: C M
authors.initials: CM
authors.lastname: Sparacino
authors.forename: G R
authors.initials: GR
authors.lastname: Velez
authors.forename: S
authors.initials: S
authors.lastname: Padilla
authors.forename: R C
authors.initials: RC
authors.lastname: MacPhail
authors.forename: K M
authors.initials: KM
authors.lastname: Crofton
mesh_terms: D020106:Acrylamide; D000178:Acrylamides / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D012584:Sciatic Nerve / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D020106:Acrylamide