[The detection of human DNA in cell hybrids by the polymerase chain reaction with universal primers: the species specificity of the amplified DNA].
The human DNA detection method is developed on the basis of DNA cross-hydridization of the amplification products obtained by the universally primed polymerase chain reaction (UP-PCR) technique. These PCR products are characterized by species-specificity in hybridization assay. Two somatic cell hybrids "human x Chinese hamster" supposed to contain the human DNA, according to selection procedure, were analysed by this method. As a result, the presence of human DNA, unable to be tested by cytological techniques, have been proven. The amplified human DNA can be mapped by this method.
Bulat
S A
SA
Filatov
M V
MV
Pantina
R A
RA
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D005784:Gene Amplification; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
D017931:DNA Primers; D004247:DNA
Vyiavlenie DNK cheloveka v kletochnykh gibridakh metodom polimeraznoĭ tsepnoĭ reaktsii s universal'nymi praĭmerami: vidospetsifichnost' amplifitsirovannoĭ DNK.