Developmental regulation of aortic elastin gene expression involves disruption of an IGF-I sensitive repressor complex.
Nuclear proteins were isolated from different aged chick embryonic aorta and examined by gel mobility shift and footprint analyses using the -195 to +2 bp region of the human elastin gene. A major developmental change in DNA/protein complexes involved the loss of binding complexes present initially in the 8- to 11-day nuclear extracts. Both chemical and enzymatic footprinting demonstrated that the deprotected binding complexes corresponded to positions -167 to -137 bp within the elastin promoter fragment. Gel shift competition assays confirmed the identity of the specific DNA sequences affected. These results demonstrate that increased elastogenesis during aortic embryogenesis involves the derepression of a negative element functionality previously shown to be affected by IGF-I in vitro.
Rich
C B
CB
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118.
Goud
H D
HD
Bashir
M
M
Rosenbloom
J
J
Foster
J A
JA
D000818:Animals; D001011:Aorta / Q000196:embryology / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D002642:Chick Embryo; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004549:Elastin / Q000096:biosynthesis* / Q000235:genetics*; D005314:Embryonic and Fetal Development; D005786:Gene Expression Regulation / Q000187:drug effects*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000196:embryology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*
D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D012097:Repressor Proteins; D007335:Insulin-Like Growth Factor II; D004247:DNA; D004549:Elastin; D003850:Deoxyribonuclease I