title: Sequence specific binding of the transcription factor c-Ets1 to the human immunodeficiency virus type I long terminal repeat.
abstract: Human immunodeficiency virus type I (HIV-1) long terminal repeat (LTR) driven transcription is regulated by a variety of cellular transcription factors. Most work has focused on the two nuclear factor kappa B (NF-kB) elements indispensable for HIV-1 LTR enhancer function. We demonstrate here the specific binding of the transcription factor c-Ets1 to an U3 region of the HIV-1 LTR (nt -141 to -149) using electrophoretic mobility shift analysis with T-cell nuclear extract and in vitro translated protein. This previously not identified Ets binding site is highly conserved among different HIV-1 isolates and maps to an U3 region recently shown to be necessary for viral growth in vitro. The c-Ets proto-oncogene family of transcription factors has yet been associated with HTLV-I and HIV-2 transcription. Our present analysis suggests an important role of c-Ets proteins in HIV-1 transcription.
authors.affiliation: Abteilung Biochemie, Robert Koch-Institut, Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Holzmeister
authors.forename: B
authors.initials: B
authors.lastname: Ludewig
authors.forename: G
authors.initials: G
authors.lastname: Pauli
authors.forename: D
authors.initials: D
authors.lastname: Simon
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma; D014176:Protein Biosynthesis; D000090063:Proto-Oncogene Mas; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: C494041:ETS1 protein, human; C000715470:MAS1 protein, human; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors