Construction of a genetic map telomeric to HLA-A by microsatellite analysis.
Allele and genotype frequencies of five microsatellites on the short arm of chromosome 6 have been determined in 158 subjects from 14 large Italian families, using polymerase chain reaction amplification and a non-radioactive method of detection. The markers studied were: D6S89, D6S109, D6S105, pLR12 and HLA-F; their heterozygosity ranged from 0.65 to 0.78. Two point and three point linkage analysis among these markers and HLA-A serological types--available for all the studied samples--allowed construction of a partial genetic map telomeric to HLA-A. Our results may be useful for linkage analysis of genetic disorders mapping on this region of 6p.
Camaschella
C
C
Dipartimento Scienze Biomediche e Oncologia Umana, Università di Torino, Italy.
Roetto
A
A
De Sandre
G
G
Piperno
A
A
Totaro
A
A
Dianzani
I
I
Gasparini
P
P
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D007558:Italy; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016615:Telomere
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers; D015234:HLA-A Antigens