An improved sedimentation technique for the cytologic preparation of cerebrospinal fluid cells.
The conventional CSF cell preparation methods suffer from a poor cell recovery rate (mean rates of 10-50% with the sedimentation techniques, which makes it unlikely, that the cell sampling on the slide is representative) or from suboptimal cell visualization (filter techniques). An improved sedimentation technique is described with a mean recovery rate of 80% and excellent cell visualization.
Seyfert
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Neurological Clinic, Klinikum Steglitz, Freie Universität Berlin, Germany.
D002448:Cell Adhesion / Q000502:physiology; D002469:Cell Separation; D002478:Cells, Cultured; D002498:Centrifugation / Q000379:methods; D002555:Cerebrospinal Fluid / Q000166:cytology*; D005260:Female; D006801:Humans; D008297:Male