Structural characterization of gangliosides from resting and endotoxin-stimulated murine B lymphocytes.
B lymphocytes from CBA/J mice were stimulated in splenocyte cultures for 72 h with various endotoxins. Bisphosphoryl lipid A from Escherichia coli had the highest stimulatory effect followed by LPS of Citrobacter freundii and Salmonella minnesota as measured by [3H]thymidine uptake. Gangliosides of stimulated B cells (metabolically labeled with D-[1-14C]galactose and D-[1-14C]glucosamine) and unlabeled gangliosides from resting B cells (prepared from spleens without stimulus) were analyzed by high-performance TLC, DEAE anion-exchange HPLC, and immunostaining procedures. Contents of ganglioside-derived sialic acids, quantified by HPLC as their fluorescent derivatives, decreased from stimulated to resident B lymphocytes in the following order: LPS S. minnesota > LPS C. freundii > bisphosphoryl lipid A E. coli > resting B cells. Gangliosides of resting B cells contained more N-glycolyl- than N-acetylneuraminic acid, whereas inverse ratios were found in activated cells, indicating a shift from N-glycolyl- to N-acetylneuraminic acid due to stimulation. Furthermore, a higher disialoganglioside content was characteristic for activated B cells. Fast atom bombardment mass spectrometry was performed with permethylated mono- and disialoganglioside fractions of LPS S. minnesota and LPS C. freundii stimulated B cells. Major gangliosides were GM1a and GD1a beside minute amounts of GD1b. The structural heterogeneity in the gangliosides was caused by (a) N-substitution of the sialic acids with either acetyl or glycolyl groups, (b) variation in the long-chain base (sphingosine, sphinganine), and (c) substitution of the ceramide moiety by fatty acids of different chain length and degree of unsaturation (C16:0, C24:0,24:1).2+ p6
Pörtner
A
A
AG Zellkulturtechnik, University of Bielefeld, Germany.
Peter-Katalinić
J
J
Brade
H
H
Unland
F
F
Büntemeyer
H
H
Müthing
J
J
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives; D005732:Gangliosides / Q000737:chemistry* / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D012794:Sialic Acids / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013154:Spleen / Q000166:cytology
D004731:Endotoxins; D005732:Gangliosides; D012794:Sialic Acids; C025447:sialogangliosides; C021298:ganglioside, GD1a; D005677:G(M1) Ganglioside