title: Laser-induced fluorescence detection of 9-fluorenylmethyl chloroformate derivatized amino acids in capillary electrophoresis.
abstract: Laser-induced fluorescence (LIF) was applied to the detection of 9-fluorenylmethyl chloroformate (FMOC-Cl) derivatized amino acids separated by capillary electrophoresis. Fluorescence excitation was provided by a pulsed, KrF laser operating at 248 nm. A limit of detection of 5 x 10(-10) M was obtained for FMOC-alanine (S/N = 2). Separation of FMOC-derivatized proline, hydroxyproline, and sarcosine was achieved with a 20 mM borate buffer (pH 9.2), and the separation of FMOC-derivatized amino acid standard mixture was obtained using a 20 mM borate buffer (pH 9.2) containing 25 mM sodium dodecyl sulfate.
authors.affiliation: Program Resources, Inc./DynCorp, NCI-Frederick Cancer Research and Development Center, MD 21702.
authors.forename: K C
authors.initials: KC
authors.lastname: Chan
authors.forename: G M
authors.initials: GM
authors.lastname: Janini
authors.forename: G M
authors.initials: GM
authors.lastname: Muschik
authors.forename: H J
authors.initials: HJ
authors.lastname: Issaq
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D005449:Fluorenes / Q000032:analysis*; D007202:Indicators and Reagents; D007834:Lasers; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000596:Amino Acids; D005449:Fluorenes; D007202:Indicators and Reagents; C054007:1-(9-fluorenyl)methyl chloroformate