Purpurogallin: in vivo evidence of a novel and effective cardioprotector.
We observed that purpurogallin (PPG) which is a flavonoid markedly protects the rabbit against myocardial ischemia-reperfusion injury. In rabbits undergoing 1-h ligation of the anterior ventricular coronary artery, a bolus infusion of PPG was introduced into the post-ischemic heart immediately before 3-h reperfusion. Against negligible necrosis in 6 sham-operated controls, and 41.7 (SD 11.3)% necrosis in the area at risk for the placebo control group (n = 14 animals), the PPG-treated groups (n = 6, 6, 14) had a necrosis of 26.8 (6.4)%, 10.8 (3.5)%, and 11.7 (5.2)% at doses of 2.5, 5, and 10 mumol/kg, respectively (each with p < 0.01 vs control value). By comparison, infusion of Trolox (a vitamin E analogue) at 5 mumol/kg produced a higher necrosis of 17.7 +/- 7.2% (n = 6, p < 0.05 vs value obtained from 5 mumol/kg PPG-treated group) in the same model. Note that myocardial necrosis was estimated by tetrazolium-based histochemistry and confirmed by light and transmission electron microscopies. These data support our contention that PPG is an effective cardioprotector, whose mechanism of action will be reported separately.
Wu
T W
TW
Department of Clinical Biochemistry, University of Toronto, Canada.
Wu
J
J
Zeng
L H
LH
Au
J X
JX
Carey
D
D
Fung
K P
KP
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001567:Benzocycloheptenes / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology; D009336:Necrosis; D011817:Rabbits
D000975:Antioxidants; D001567:Benzocycloheptenes; C026133:purpurogallin