title: Differential effect of transporter Tap 2 gene introduction into RMA-S cells on viral antigen processing.
abstract: The protein products of the Tap (Transporter associated with antigen processing) 1 and 2 genes are presumed to deliver peptides across the endoplasmic reticulum (ER) for assembly with major histocompatibility complex (MHC) class I molecules. The antigen processing-defective cell line RMA-S (H-2b) has a premature stop in the Tap 2 gene and probably therefore fails to deliver peptides into the ER, which leads to a low level of cell surface MHC class I molecules. Transfection of a Tap 2 gene restores to RMA-S both MHC class I molecule expression and the ability to present influenza viral antigens. We investigated the ability of RMA-S cells transfected with a Tap 2 gene to process and present alloantigens, Sendai and Rauscher viral antigens to allogeneic and virus-specific cytotoxic T lymphocytes. We found that allogeneic peptides as well as Rauscher and Sendai viral peptides can be processed and presented by RMA-S but at reduced levels. Transfection of a Tap 2 gene of mouse (BALB/c, H-2d) or rat origin into RMA-S increased the presentation of Sendai viral antigens and partially restored the presentation of allogeneic antigens. The already low level of Rauscher viral peptides presented by RMA-S is not elevated by transfection of either Tap 2 gene into RMA-S. This indicates a differential effect of transfection of a Tap 2 gene of rat or allogeneic mouse origin into RMA-S on viral antigen processing.
authors.affiliation: Division of Immunohaematology, University Hospital, Leiden, The Netherlands.
authors.forename: M A
authors.initials: MA
authors.lastname: Ossevoort
authors.forename: A J
authors.initials: AJ
authors.lastname: Sijts
authors.forename: K J
authors.initials: KJ
authors.lastname: van Veen
authors.forename: F
authors.initials: F
authors.lastname: Momburg
authors.forename: G J
authors.initials: GJ
authors.lastname: Hämmerling
authors.forename: A
authors.initials: A
authors.lastname: Seelig
authors.forename: G W
authors.initials: GW
authors.lastname: Butcher
authors.forename: J C
authors.initials: JC
authors.lastname: Howard
authors.forename: W M
authors.initials: WM
authors.lastname: Kast
authors.forename: C J
authors.initials: CJ
authors.lastname: Melief
mesh_terms: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D051381:Rats; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism; D014162:Transfection
chemical_list: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D000956:Antigens, Viral; D002352:Carrier Proteins; D000949:Histocompatibility Antigens Class II; C489516:Tap2 protein, mouse; C489514:Tap2 protein, rat; C071692:TAP2 protein, human