[The frequency of sister chromatid exchanges in preimplantation mouse embryos].
The frequency of sister-chromatid exchanges (SCE) was analysed in spontaneously and superovulated morulae and blastocyst of CBA, C57Bl, F1 CBA x C57Bl mice in culture with bromodeoxyuridine (BrdU). The background level for SCE was found to be 4-5 times higher in early embryos than in fetal or adult tissues of the mouse. The phenomenon of spontaneous sister-chromatid differentiation (SCD) of blastocyst chromosomes without any treatment with BrdU was observed. The SCE frequency was calculated on such chromosomes and was the same as with BrdU. A possible mechanism of this phenomenon is suggested.
Patkin
E L
EL
Kustova
M E
ME
Dyban
A P
AP
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000648:ultrastructure; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002871:Chromosome Banding; D003433:Crosses, Genetic; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects / Q000648:ultrastructure*; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009028:Morula / Q000187:drug effects / Q000648:ultrastructure; D011247:Pregnancy; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013480:Superovulation / Q000187:drug effects
D001973:Bromodeoxyuridine
Chastota sestrinskikh khromatidnykh obmenov v doimplantatsionnykh zarodyshakh mysheĭ.