title: Lipopolysaccharide-induced change of ADP-ribosylation of a cytosolic protein in bone-marrow-derived macrophages.
abstract: Treatment of bone-marrow-derived macrophages with nanogram quantities of bacterial lipopolysaccharide (LPS) or with the synthetic bacterial lipopeptide analogue N-palmitoyl-(S)-[2,3-bis(palmitoyloxy)-(2RS)-propyl] (Pam3)Cys-Ala-Gly results in a change of ADP-ribosylation of a cytosolic 33 kDa protein. The immunostimulant-induced change is both dose- and time-dependent. It is not observed in macrophages from an LPS-unresponsive C3H/HeJ mouse strain upon treatment with LPS. Non-endotoxic LPS from Rhodopseudomonas pallustris, the inactive lipopeptide analogue Pam3CysOH, and LPS in the presence of polymyxin B fail to induce the change of ADP-ribosylation of the protein. These observations indicate that reversible protein modification by ADP-ribosylation might play a role in macrophage activation.
authors.affiliation: Institut für Immunbiologie der Universität, Freiburg, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hauschildt
authors.forename: P
authors.initials: P
authors.lastname: Scheipers
authors.forename: W G
authors.initials: WG
authors.lastname: Bessler
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008074:Lipoproteins / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009569:Nitric Oxide / Q000378:metabolism; D011112:Polymyxin B / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D008074:Lipoproteins; D011506:Proteins; C061110:N-palmitoyl-5,6-dipalmitoylcysteinyl-alanyl-glycine; D000246:Adenosine Diphosphate Ribose; D009569:Nitric Oxide; D011112:Polymyxin B