Simple, inexpensive suction electrode system for the student physiology laboratory.
A versatile, inexpensive, easily constructed suction electrode system is described that can be used for routine exercises by the student on, or for demonstrations of, the electrophysiology of frog sciatic nerve and gastrocnemius and sartorius muscles. A disposable plastic syringe and a disposable pipette with Ag-AgCl wire comprise the suction electrode. Six readily manipulable electrodes are installed in the walls of the Plexiglas preparation bath, and an arbitrary number of additional "free" electrodes can be placed where desired. An optional small chamber, formed from a disposable plastic culture dish and Gootch rubber tubing, provides for "barrier" recording within the larger chamber. Interpretations are given of the electrical responses of nerve and muscle in terms of longitudinal currents along the preparation within the suction electrode tubes.
Easton
D M
DM
Department of Biological Science, Florida State University, Tallahassee 32306.
D000818:Animals; D004566:Electrodes; D004867:Equipment Design; D066298:In Vitro Techniques; D009469:Neuromuscular Junction / Q000502:physiology; D010827:Physiology / Q000193:education / Q000295:instrumentation*; D011898:Ranidae; D012965:Sodium Chloride; D012996:Solutions; D013396:Suction / Q000295:instrumentation; D009435:Synaptic Transmission / Q000502:physiology; D013664:Teaching Materials
D012996:Solutions; D012965:Sodium Chloride