title: [Methane fermentation of cattle manure at low temperature].
abstract: The acclimated microbial association fermenting cattle manure at 6 degrees C with methane production was obtained by a long-time (2.5 years) autoselection. It is shown that the basic barrier to carry out this process at low temperature is long-time period of adaptation of microbial community. It is removed by addition of the psychrophilic barm obtained. After cultivating of this psychrophilic methanogenic association under mesophilic (35 degrees) and thermophilic (55 degrees) conditions its activity decreased to 2 and 10 times, respectively. Based on the kinetic estimations it was proposed, that the essential changes of properties of the anaerobic microflora investigated was occurred during the acclimation to low temperature. The psychrotrophic microorganisms played the main role in the process of organic matter degradation at low temperature in the studied system. The pure cultures of saccharolitic and acetogenic psychrotrophic bacteria those are capable to grow in the wide range of temperature, from 0 degrees to 35 degrees C were isolated. Methanosarcina and methanothrix ix were detected in the enrichment cultures growing with acetate at 6 degrees C.
authors.forename: O R
authors.initials: OR
authors.lastname: Kotsiurbenko
authors.forename: A N
authors.initials: AN
authors.lastname: Nozhevnikova
authors.forename: S V
authors.initials: SV
authors.lastname: Klaiuzhnyĭ
authors.forename: G A
authors.initials: GA
authors.lastname: Zavarzin
mesh_terms: D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D019605:Euryarchaeota / Q000502:physiology*; D005285:Fermentation / Q000502:physiology*; D008372:Manure
chemical_list: D008372:Manure
vernacular_title: Metanovoe sbrazhivanie navoza krupnogo rogatogo skota v psikhrofil'nykh usloviiakh.