title: Immunocytochemical localization of glucocorticoid receptors in the spinal cord: effects of adrenalectomy, glucocorticoid treatment, and spinal cord transection.
abstract: 1. Studies were performed to determine the changes in immunoreactive (IR) type II glucocorticoid receptors of the ventral horn of the spinal cord produced by adrenalectomy (ADX), dexamethasone (DEX) treatment, and spinal cord transection in rats. 2. These treatments did not significantly affect the number of IR neurons of the ventral horn; however, staining intensity was enhanced after ADX and decreased following 4 days of DEX. A similar response pattern was observed for glial-type cells. 3. In control rats, about half of the ventral horn motoneurons were surrounded by immunoreactive glial perineuronal cells. These perineuronal cells increased after ADX (77% of counted neurons) and decreased following DEX treatment (32%; P < 0.05). 4. Two days after transection, staining was intensified in ventral horn motoneurons and glial cells located in the spinal cord below the lesion. Immunoreactive perineuronal cells increased to 85% of counted neurons, from a value of 66% in sham-operated rats (P < 0.05). 5. These findings suggest considerable plasticity of the spinal cord GCR in response to changes in hormonal levels and experimental lesions. It is possible that factors involved in cell to cell communication with transfer of hypothetical regulatory molecules may play roles in GCR regulation and the increased immunoreaction of glia associated with neurons following transection and ADX.
authors.affiliation: Laboratory of Neuroendocrine Biochemistry, Instituto de Biologia y Medicina Experimental, Buenos Aires, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Ferrini
authors.forename: S
authors.initials: S
authors.lastname: González
authors.forename: T
authors.initials: T
authors.lastname: Antakly
authors.forename: A F
authors.initials: AF
authors.lastname: De Nicola
mesh_terms: D000315:Adrenalectomy; D000704:Analysis of Variance; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values; D013116:Spinal Cord / Q000378:metabolism* / Q000502:physiology
chemical_list: D011965:Receptors, Glucocorticoid; D003907:Dexamethasone