Antibodies to casein kinase II in sera of patients with mixed connective tissue disease: evaluation with recombinant proteins.
In this study we determined the prevalence of autoantibodies against casein kinase II (CKII) in patients positive for anti-70K marker antibodies, which is indicative of mixed connective tissue disease. An anti-CKII ELISA was established with the use of bacterially expressed recombinant CKII proteins. Eight out of 52 anti-70K-positive sera (15%) were positive for anti-CKII antibodies, which recognized preferentially the CKII alpha subunit. All control sera (n = 52) were anti-CKII negative. Thus, the occurrence of anti-CKII antibodies may be of value for differential diagnosis.
Wiemann
C
C
Biochemical Cell Physiology, German Cancer Research Center, Heidelberg.
Bodenbach
L
L
Pyerin
W
W
D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood*; D047390:Casein Kinase II; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008947:Mixed Connective Tissue Disease / Q000276:immunology*; D017346:Protein Serine-Threonine Kinases / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D001323:Autoantibodies; D011994:Recombinant Proteins; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases