PEC-60, a novel regulatory peptide reduces cyclic AMP formation in rat caudate nucleus.
PEC-60, a sixty-residue novel regulatory peptide with N-terminal glutamic acid and C-terminal cysteine is abundant in intestinal tissue. PEC-60-like immunoreactivity is found in catecholamine neurons and intracerebroventricular injections of PEC-60 reduce dopamine utilization within the caudate nucleus indicating a possible role of this peptide in the central nervous system. We have investigated the effect of PEC-60 on cAMP formation in membrane preparations from rat caudate nucleus. We have found that PEC-60 significantly and dose-dependently reduces basal and forskolin stimulated cAMP production. The results demonstrate for the first time the interaction of PEC-60 with specific binding sites that regulate adenylate cyclase in inhibitory fashion in rat caudate nucleus.
Laasik
J
J
Institute of Biochemistry, Tartu University, Estonia.
Sillard
R
R
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002421:Caudate Nucleus / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D007422:Intestines; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013552:Swine
C069343:PEC-60 polypeptide; D010455:Peptides; D005576:Colforsin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004298:Dopamine