title: Phosphatidylserine specific binding protein in rat brain: purification and characterization.
abstract: A calcium-independent phosphatidylserine specific binding protein detected on liposome blotting analysis was purified from rat brain and revealed to be identical to myristoylated, alanine-rich C kinase substrate (MARCKS). MARCKS specifically binds to phosphatidylserine but not phosphatidylcholine. The binding of MARCKS to phosphatidylserine was abolished on protein kinase C-dependent phosphorylation. Since bacterially expressed MARCKS also specifically binds to phosphatidylserine, myristoylation of the N-terminal glycine seems not to be essential for the binding of MARCKS to phosphatidylserine. These data suggest that phosphatidylserine is a membranous target molecule of MARCKS.
authors.affiliation: Glyco Molecular Biology, Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Saitama.
authors.forename: T
authors.initials: T
authors.lastname: Nakaoka
authors.forename: N
authors.initials: N
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Hamamoto
authors.forename: N
authors.initials: N
authors.lastname: Kurosawa
authors.forename: Y C
authors.initials: YC
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Kawasaki
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002793:Cholic Acids; D047908:Intracellular Signaling Peptides and Proteins; D008081:Liposomes; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D010718:Phosphatidylserines / Q000378:metabolism*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
chemical_list: D002793:Cholic Acids; D047908:Intracellular Signaling Peptides and Proteins; D008081:Liposomes; C000619712:Marcks protein, rat; D008565:Membrane Proteins; D010718:Phosphatidylserines; D011506:Proteins; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate