Effect of prostaglandin E2 on cellular, lysosomal and mitochondrial fragility in caerulein-induced pancreatitis in rats.
The effects of prostaglandin E2 on the fragility of cellular and subcellular organelles in caerulein-induced acute pancreatitis were investigated in rats. PGE2 at doses of 50 and 100 micrograms/kg/hr infused for 2 hours before and during caerulein (5 micrograms/kg/hr for 3.5 hours) infusion significantly prevented the increased discharge of both amylase and lactate dehydrogenase from dispersed acini, and the leakage of cathepsin B from lysosomes and of malate dehydrogenase from mitochondria in the subcellular fraction in vitro. These results suggest that PGE2 has a cytoprotective effect against caerulein-induced pancreatitis by stabilizing cell and lysosomal and mitochondrial membranes.
Manabe
T
T
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Hirano
T
T
Ando
K
K
Yotsumoto
F
F
Tobe
T
T
D000208:Acute Disease; D000818:Animals; D002401:Cathepsin B / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects*; D002108:Ceruletide; D015232:Dinoprostone / Q000494:pharmacology*; D004195:Disease Models, Animal; D066298:In Vitro Techniques; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000187:drug effects*; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar
D002108:Ceruletide; D008291:Malate Dehydrogenase; D002401:Cathepsin B; D015232:Dinoprostone