title: Phosphorylation enhances inactivation of N-type calcium channel current in bullfrog sympathetic neurons.
abstract: We have investigated the effects of phosphatase and protein kinase inhibitors on calcium channel currents of bullfrog sympathetic neurons using the whole cell configuration of the patch clamp technique. Intracellular dialysis with the phosphatase inhibitors okadaic acid and calyculin A markedly enhanced the decline of inward current during a depolarizing voltage step. Tail current analysis demonstrated that this was genuine inactivation of calcium channel current, not activation of an outward current. The rapidly inactivating current is N-type calcium current (blocked by omega-conotoxin and resistant to nifedipine). Staurosporine, a nonselective protein kinase inhibitor, prevented the action of okadaic acid, suggesting that protein phosphorylation is involved. Under control conditions, the time course of inactivation could be described by the sum of two exponentials (tau = 150 ms and 1200 ms), plus a constant (apparently noninactivating) component, during depolarizations lasting 2 s. Okadaic acid induced a rapid inactivation process (tau = 15 ms) that was absent or negligible under control conditions, without obvious effect on the two slower time constants. As in control cells, inactivation in okadaic-acid-treated cells was strongest near -20 mV, with less inactivation at more positive voltages. However, inactivation did not depend on calcium influx. Modulation of calcium channel activity by phosphorylation may underly the spontaneous shift between inactivating and noninactivating modes recently observed for N-type calcium channels. Differences in basal phosphorylation levels could also explain why N-type calcium channels, originally described as rapidly and completely inactivating, inactivate slowly and incompletely in many neurons.
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106.
authors.forename: M A
authors.initials: MA
authors.lastname: Werz
authors.forename: K S
authors.initials: KS
authors.lastname: Elmslie
authors.forename: S W
authors.initials: SW
authors.lastname: Jones
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers; D015220:Calcium Channels / Q000378:metabolism*; D004988:Ethers, Cyclic / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism*; D019319:Okadaic Acid; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D011892:Rana catesbeiana; D013564:Sympathetic Nervous System / Q000166:cytology / Q000378:metabolism*
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D004988:Ethers, Cyclic; D047428:Protein Kinase Inhibitors; D019319:Okadaic Acid; D011494:Protein Kinases; D010744:Phosphoric Monoester Hydrolases