title: Isolation and characterization of novel genes which are expressed during the very early stage of zygote formation in Chlamydomonas reinhardtii.
abstract: We have obtained five cDNA clones which are complementary to transcripts that accumulate specifically during the early stage of zygote formation in Chlamydomonas reinhardtii. Based on the time-course of transcript accumulation of their corresponding genes, these clones were classified into two groups: an early expression group (three clones; pZS102-1, pZS102-69, pZS1802) and a late expression group (two clones; pZS1860 and pZS1886). The levels of transcripts in the former group reached a maximum 30 min after the onset of mating, whereas those in the latter group peaked at 3 h. The addition of 10 micrograms/ml of cycloheximide reduced the decline in the levels of transcripts, except in the transcript corresponding to pZS102-69. Two clones, pZS102-1 and pZS102-69, contained highly-homologous predicted open-reading frames, and were judged to be cDNAs for two members of a novel gene family. These genes were referred to as zys1A and zys1B, respectively.
authors.affiliation: Department of Botany, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Uchida
authors.forename: S
authors.initials: S
authors.lastname: Kawano
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Kuroiwa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000254:growth & development; D004247:DNA; D008969:Molecular Sequence Data; D005810:Multigene Family; D014158:Transcription, Genetic; D015053:Zygote / Q000378:metabolism*
chemical_list: D004247:DNA