The effect of phorbol ester treatment on migration of C3H 10T1/2 and BT5C glioma cells: possible application to carcinogenesis.
Treatment with the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and the second-stage promoter 12-O-retinoylphorbol 13-acetate induced down-regulation of protein kinase C (PKC) and enhanced the migration of C3H 10T1/2 cells. In the case of BT5C glioma cells the same negative correlation was observed only after treatment with TPA. The negative control 4 alpha-phorbol affected neither PKC activity nor the migratory ability of both cell lines.
Janik
P
P
Department of Tumor Biology, Cancer Center, Warsaw, Poland.
Szaniawska
B
B
Kowalczyk
D
D
Miłoszewska
J
J
D000818:Animals; D002460:Cell Line / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D005910:Glioma / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D010703:Phorbol Esters / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D010703:Phorbol Esters; D011493:Protein Kinase C