title: Two putative African swine fever virus helicases similar to yeast 'DEAH' pre-mRNA processing proteins and vaccinia virus ATPases D11L and D6R.
abstract: Two open reading frames (ORFs) of African swine fever virus (ASFV) encoding putative helicases have been sequenced. The two genes, termed D1133L and B962L, are located in the central region of the viral genome, but are separated by about 40 kb of DNA. Both genes are expressed late during ASFV infection of Vero cells, after replication of viral DNA has begun. Contiguous to D1133L, three other ORFs (D129L, D79L and D339L), encoding putative proteins of unknown function, have been sequenced. Proteins D1133L and B962L contain the amino acid motifs that characterize helicases of superfamily II. D1133L is most similar to a group of putative helicases which includes two proteins of vaccinia virus (D11L and D6R) involved in transcription of the viral genome, their homologues in other poxviruses, the protein encoded by ORF 4 of the yeast plasmids, pGKL2 and pSKL, and the previously identified ASFV protein, Q706L. B962L resembles a group of RNA-helicase-like proteins which includes three proteins of Saccharomyces cerevisiae involved in pre-mRNA splicing (PRP2, PRP16 and PRP22), Drosophila melanogaster KURZ and MLE, and vaccinia virus 18R.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
authors.forename: R J
authors.initials: RJ
authors.lastname: Yáñez
authors.forename: J M
authors.initials: JM
authors.lastname: Rodríguez
authors.forename: M
authors.initials: M
authors.lastname: Boursnell
authors.forename: J F
authors.initials: JF
authors.lastname: Rodríguez
authors.forename: E
authors.initials: E
authors.lastname: Viñuela
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000358:African Swine Fever Virus / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004265:DNA Helicases / Q000235:genetics*; D004279:DNA, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020365:RNA Helicases; D012323:RNA Processing, Post-Transcriptional; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014616:Vaccinia virus / Q000201:enzymology*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D000251:Adenosine Triphosphatases; D004265:DNA Helicases; C084939:B962L protein, African swine fever virus; C084940:D1133L protein, African swine fever virus; D020365:RNA Helicases