Preservation of peripheral nerve grafts with Schwann cell culture medium.
Preservation of peripheral nerves may, in the near future, play an important role in reconstructive surgery, especially if recent advances in immunosuppressive therapy are taken into account. Therefore, it has to be investigated whether peripheral nerves can be stored for some time after harvesting without diminishing their regenerative potential. Previous experiments of our group could demonstrate only little benefit of organ storage solution (HTK) or normal saline (NaCl 0.9%) to peripheral nerves when kept at cold ischaemia of 4 degrees C for 32 and 72 hours. In this presentation, we are reporting the results of peripheral nerve storage in Dulbecco's Modified Eagle Medium which has been used for Schwann cell culture. In 30 adult Sprague-Dawley rats, a 2.5 cm segment of the right sciatic nerve was harvested and kept at 4 degrees C for 14, 32, 72, and 120 hours. It was then reimplanted into the donor animal; regeneration quality was assessed clinically, histologically and morphometrically after 6 weeks. Best regeneration results were obtained in the 32 and 72 hour groups; regeneration here was comparable to the normal controls. These results are explained with the positive effect of nerve predegeneration.
Lassner
F
F
Clinic for Plastic, Hand and Reconstructive Surgery, Medical School Hannover, Germany.
Becker
M
M
Fansa
H
H
Schaller
E
E
Berger
A
A
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D003470:Culture Media; D009416:Nerve Regeneration; D010525:Peripheral Nerves / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000502:physiology*; D012584:Sciatic Nerve / Q000637:transplantation; D014021:Tissue Preservation / Q000379:methods*
D003470:Culture Media