title: Heat resistance of Listeria monocytogenes in vacuum packaged pasteurized fish fillets.
abstract: The heat resistance of two strains of Listeria monocytogenes in sous-vide cooked fillets of cod and salmon was investigated. Fish sticks of 5 g were inoculated, vacuum-packed and heated at different combinations of time and temperature (58-80 degrees C). Time-temperature combinations allowing survival and time-temperature combinations at which the bacteria were destroyed, were used to determine D- and z-values. D-values were in the range of what has been published for other food products. D60-values were between 1.95 and 4.48 min depending on the strain and the fish. Both strains were one-four-times more heat resistant in salmon than in cod, showing the importance of the heating menstruum. This difference may be due to the higher fat content in salmon as compared to cod. Z-values were calculated to be 5.65 and 6.4 degrees C, respectively, for the two strains. The suitability of methods for heat resistance experiments and the survival of L. monocytogenes in sous-vide cooked fish fillets are discussed.
authors.affiliation: Technological Laboratory, Danish Ministry of Fisheries, Technical University, Lyngby.
authors.forename: P K
authors.initials: PK
authors.lastname: Ben Embarek
authors.forename: H H
authors.initials: HH
authors.lastname: Huss
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D005519:Food Preservation; D006358:Hot Temperature; D008089:Listeria monocytogenes / Q000254:growth & development*; D012474:Salmon / Q000382:microbiology; D013997:Time Factors