[The role of Ca(2+)-ATPase and oxygen radical in reperfusion injury of rat liver].
The effects of ischemia and reperfusion with and without oxygen radical scavengers and xanthine oxidase inhibitors on Ca(2+)-ATPase activity were examined in the rat liver of 5 min ischemia followed by 5 and 10 min reperfusion. Ischemia was produced by the ligation of right hepatic artery and right portal vein. Superoxide dismutase, catalase and allopurinol were administered by subcutaneous injection of 60,000U/kg, 90,000U/kg and 200mg/kg, respectively before ligation. Reaction products of Ca(2+)-ATPase were morphometrically analyzed by RUZEX IIIU. Histochemically, Ca(2+)-ATPase activities were demonstrated on plasma membrane of liver cells, bile canaliculi and Kupffer cells involving mitochondria in liver cells of control rats. Ca(2+)-ATPase activities were depressed in the central lobes of liver after 5 min ischemia followed by 5 and 10min reperfusion. However, the activities of Ca(2+)-ATPase were not depressed by addition of oxygen radical scavengers and xanthine oxidase inhibitor before ischemia. These results suggest that oxygen free radicals may influence Ca(2+)-ATPase activity and contribute to liver cell damage due to ischemia-reperfusion.
Watanabe
H
H
First Department of Surgery, Nippon Medical School, Tokyo, Japan.
Onda
M
M
Genga
A
A
Asano
G
G
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism / Q000502:physiology*; D002374:Catalase / Q000494:pharmacology; D005609:Free Radicals; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply*; D008297:Male; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000502:physiology
D005609:Free Radicals; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D000252:Calcium-Transporting ATPases; D010100:Oxygen