title: Tissue carriers for processing fragile bio-materials in immuno-electron microscopy.
abstract: The preparation and use of tissue carriers for processing fragile biological materials from postfixation up to embedding in electron microscopy is described. Trimmed small pieces of vibratome sections can be very practical and safely transported in these carriers throughout osmification, dehydration for long times, and infiltration up to embedding in epoxy resins. This small device is especially suited for post-embedding immunogold electron microscopy.
authors.affiliation: Department of Histology and Embryology, Tongji Medical University, Wuhan, P.R. China.
authors.forename: Q
authors.initials: Q
authors.lastname: Peng
authors.forename: H P
authors.initials: HP
authors.lastname: Richter
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D008473:Median Eminence / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron / Q000295:instrumentation*; D008867:Microtomy; D011138:Polytetrafluoroethylene; D051381:Rats; D016610:Tissue Embedding / Q000295:instrumentation*
chemical_list: D011138:Polytetrafluoroethylene