[The effect of pro-oxidants on insulin secretion by the isolated rat pancreas].
The effect of pro-oxidants (tert-butyl peroxide and FeSO4) on rats perfusing isolated pancreas insulin secretion was studied. Pro-oxidants (10(-4) M) preperfusion during 20 minutes did not change significantly the basal insulin secretion and decreased glucose-stimulated secretion of the one. The content of pancreas TBA-active products increased twice after pro-oxidants effect and ratio of reduced and oxidized glutathiones liberation rates decreased from 2 to 0.5. The disturbance of hormone secretion as a result of decrease of Langerhans islets B-cells reduced glutathione pool is suppose to be one of the mechanisms of hypoinsulinemia development at host's states characterizing with lipids peroxidation activation.
Ivanov
V V
VV
Lusta
I V
IV
Sitozhevskiĭ
A V
AV
Trofimov
A V
AV
Kozhanova
A A
AA
D000818:Animals; D005296:Ferrous Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D016877:Oxidants / Q000494:pharmacology; D010477:Perfusion / Q000379:methods; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D013997:Time Factors; D020122:tert-Butylhydroperoxide
D005296:Ferrous Compounds; D007328:Insulin; D016877:Oxidants; D010545:Peroxides; D017382:Reactive Oxygen Species; C020748:ferrous sulfate; D020122:tert-Butylhydroperoxide
Vliianie prooksidantov na sekretsiiu insulina izolirovannoĭ podzheludochnoĭ zhelezoĭ krys.