A new method of long-term bile collection from unrestrained dogs.
A method of long-term bile collection from unrestrained dogs was developed. A steel needle was passed subcutaneously from the animal's right side into the abdomen. A catheter, threaded through this needle, was inserted from the side and brought out below the abdomen. The catheter was introduced into the common bile duct and a vest was then fitted on the dog. The external catheter tip was placed in a collection bottle in the pocket of the vest. This method allows the animal to move freely in its cage. The bile of an unrestrained dog weighing 10 kg was collected for 3 weeks at an output rate of 4.2-4.6ml per hour.
Takaichi
M
M
Tokai Research Laboratories, Daiichi Pure Chemicals Co. Ltd., Ibaraki, Japan.
Nanbo
T
T
D000818:Animals; D001646:Bile; D002404:Catheterization; D004285:Dogs; D009068:Movement; D012149:Restraint, Physical; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*