Immunogenicity of tetanus toxoid conjugates of anti-idiotypes that mimic Pseudomonas aeruginosa surface polysaccharides.
Pseudomonas aeruginosa continues to be a serious pathogen in humans, and there is no commercially available vaccine. We have previously developed monoclonal anti-idiotypic antibodies that mimic two surface polysaccharide epitopes of pseudomonas--the high-molecular-weight polysaccharide of the O side chain of immunotype 1 lipopolysaccharide and mucoid exopolysaccharide. We now show that conjugation of the anti-idiotypes to the carrier tetanus toxoid is not necessary to induce antipolysaccharide antibodies of equal or greater titer than the native polysaccharide antigen in mice.
Schreiber
J R
JR
Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Dahlhauser
P
P
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D000922:Immunotoxins / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D007130:Immunoglobulin Idiotypes; D000922:Immunotoxins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D013745:Tetanus Toxoid