Effect of gangliosides in the autoimmune response induced by liposome-associated antigens.
A model of autoimmunity to rat male accessory glands (RAG) was recently developed by intraperitoneal administration of three doses of native RAG associated with liposomes. In this work we analysed the effects of gangliosides in the cellular response to RAG when they were intraperitoneally administrated prior to the second dose of liposome-associated RAG. Results show that the ganglioside treatment could modify an established DTH response. Also, gangliosides markedly reduced the number of Ia antigen-positive peritoneal exudated cells (PEC). However, they modified neither the processing of liposomes through PEC nor their viability. Moreover, we obtained cellular response by transferring PEC from immunized donors into naive receptors.
Correa
S G
SG
Departamento de Bioquímica Clínica, Universidad Nacional de Córdoba, Argentina.
Rivero
V E
VE
Yranzo-Volonté
N
N
Romero-Piffiguer
M
M
Ferro
M E
ME
Riera
C M
CM
D000818:Animals; D000954:Antigens, Surface / Q000187:drug effects; D015551:Autoimmunity / Q000187:drug effects*; D005260:Female; D005732:Gangliosides / Q000494:pharmacology*; D005837:Genitalia, Male / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000175:diagnosis; D008081:Liposomes / Q000276:immunology*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000637:transplantation; D008297:Male; D008856:Microscopy, Fluorescence; D010587:Phagocytosis / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar
D000954:Antigens, Surface; D005732:Gangliosides; D008081:Liposomes; D008263:Macrophage Migration-Inhibitory Factors