Synthesis and characterization of a penicillin-poly(L-lysine) which recognizes human IgE anti-penicillin antibodies.
Conjugates of poly(L-lysine) containing a penicillin or a penicilloyl residue were prepared and characterized by 1H NMR and by size-exclusion HPLC. These conjugates and conjugates with human serum albumin were used in radioallergosorbent tests (RAST) for the determination of allergy toward penicillins.
Caneva
E
E
Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy.
Di Gennaro
P
P
Farina
F
F
Orlandi
M
M
Rindone
B
B
Falagiani
P
P
D000906:Antibodies / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000276:immunology*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D010400:Penicillin G / Q000276:immunology; D010406:Penicillins / Q000138:chemical synthesis* / Q000276:immunology; D011107:Polylysine / Q000138:chemical synthesis* / Q000276:immunology; D011852:Radioallergosorbent Test; D012709:Serum Albumin / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D007202:Indicators and Reagents; D010406:Penicillins; D012709:Serum Albumin; D011107:Polylysine; D007073:Immunoglobulin E; D010400:Penicillin G