Newer approaches in treating chronic myelogenous leukemia.
We evaluated the recovery of human hematopoietic progenitors in long-term bone marrow culture (LTBMC) initiated in tissue culture (TC) flasks to that in "Lifecell" bags, which are gas-permeable plastic bags in which feeder-layer cells cannot adhere. Cells were incubated in presence of IL-1 and IL-3. Our experiments reveal sustained hematopoietic stem cell growth in the absence of a feeder layer in plastic gas-permeable bags. Evaluations of marrow from patients with chronic myelogenous leukemia suggests enrichment of normal hematopoietic precursors. Combining effective drugs to decrease Ph(+) clone prior to bone marrow harvest and use of cultured bone marrow may provide a useful method for treating patients with chronic myelogenous leukemia.
Gulati
S C
SC
Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021.
Lemoli
R
R
Van Poznak
C
C
D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D046508:Culture Techniques / Q000295:instrumentation*; D005240:Feasibility Studies; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000601:surgery*; D010969:Plastics; D011994:Recombinant Proteins / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D010969:Plastics; D011994:Recombinant Proteins