Influence of glucose on the deoxyglucose metabolism in S cerevisiae: detection and identification of deoxyglucose and trehalose derivatives by 1H- and 13C-NMR spectroscopy.
The metabolism of 2-deoxy-D-glucose (DG) in glucose grown repressed Saccharomyces cerevisiae cells was studied in the absence and presence of glucose (Glc) at 30 degrees C in a standard pyrophosphate medium containing 4.5 x 10(7) cells/ml. 1H- and 13C-NMR spectroscopy were successfully used to distinguish and identify several derivatives of DG and trehalose. Using [1-13C]DG, alpha- and beta-DG, alpha- and beta-DG6P, dideoxy-trehalose (DG-DG) and deoxy-trehalose (DG-Glc) can be simultaneously observed in the intracellular medium. The [DG6P]/[DG] ratio is about 5-6. The results seem to indicate the existence of an equilibrium between DG and DG6P, which limits the production of DG6P in cells. Glucose was found to exert a great influence on the metabolism of DG. It favours the formation of DG-DG and DG-Glc.
Wietzerbin
J
J
Département de Biologie Cellulaire et Moléculaire, CEN Saclay, Gif-sur-Yvette, France.
Hervé
M
M
Un
S
S
Neumann
J M
JM
Namane
A
A
Tran-Dinh
S
S
D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003847:Deoxyglucose / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014199:Trehalose / Q000378:metabolism*
D002247:Carbon Isotopes; D006859:Hydrogen; D003847:Deoxyglucose; D014199:Trehalose; D005947:Glucose