Cultured retinal pigment epithelial cells from RCS rats express an increased calcium conductance compared with cells from non-dystrophic rats.
The Royal College of Surgeon (RCS) rats suffer from a retinal dystrophy that is caused by a malfunction of the retinal pigment epithelium (RPE). We compared the membrane currents of cultured RPE cells from non-dystrophic and RCS rats by using the whole-cell configuration of the patch-clamp technique. Cultured RPE cells from RCS rats showed voltage-dependent, barium- and 4-aminopyridine-sensitive outward currents, which had characteristics of the delayed-rectifier and voltage-dependent, barium- and 4-aminopyridine-sensitive inward currents, which had characteristics of the inward rectifier. Differences between RPE cells from RCS rats and normal rats were as follows. (a) Cultured RCS rat RPE cells showed a resting potential and an activation threshold for the voltage-dependent outward current significantly more positive than that found in cells from non-dystrophic rats. (b) In the presence of 10 mM barium, the voltage-dependent outward current was reduced in both types of cells; in cells from RCS rats, an additional voltage-dependent inward current was observed. (c) This additional inward current had characteristics of L-type calcium channels and was reduced by verapamil (30 microM) and diltiazem (30 microM). In summary, we conclude that the membrane conductances of RPE cells from normal and RCS rats are dominated by potassium conductances. In contrast to cells from non-dystrophic rats, cells of RCS rats expressed an increased membrane conductance for calcium.
Strauss
O
O
Battelle-Institute e. V., Department of Neurobiology, Frankfurt/Main, Germany.
Wienrich
M
M
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D004553:Electric Conductivity; D010857:Pigment Epithelium of Eye / Q000502:physiology*; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D011922:Rats, Mutant Strains; D012162:Retinal Degeneration / Q000503:physiopathology*
D002121:Calcium Channel Blockers; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D001464:Barium; D015761:4-Aminopyridine; D002118:Calcium