Sodium nitroprusside promotes NAD+ labelling of a 116 kDa protein in NG108-15 cell homogenates.
A 116 kDa protein in NG108-15 homogenates is labelled in the presence of [32P]NAD+. This protein was found to be poly(ADP-ribosyl)ated and appears to be a poly(ADP-ribosyl)transferase which has poly(ADP-ribosyl)ated itself. Sodium nitroprusside, an NO generating agent, also stimulates the labelling of this protein by [32P]NAD+, but this can only be seen in the presence of thymidine, which inhibits poly(ADP-ribosyl)transferase activity. Sodium nitroprusside also stimulates the labelling of this protein by [3H-nicotinamide]NAD+, indicating that NO facilitates the formation of an adduct between this protein and NAD+. The insensitivity of the linkage between the protein and NAD+ to mercuric ions indicates that the adduct does not involve thiol groups.
Boyd
R S
RS
Department of Clinical Pharmacology, Royal Postgraduate Medical School, London, UK.
Donnelly
L E
LE
Allport
J R
JR
MacDermot
J
J
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005910:Glioma; D006822:Hybrid Cells; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism*; D009447:Neuroblastoma; D009599:Nitroprusside / Q000494:pharmacology*; D010761:Phosphorus Radioisotopes; D011065:Poly(ADP-ribose) Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
D010761:Phosphorus Radioisotopes; D009243:NAD; D009599:Nitroprusside; D011065:Poly(ADP-ribose) Polymerases