title: Caldecrin proform requires trypsin activation for the acquisition of serum calcium-decreasing activity.
abstract: Proform serum calcium-decreasing factor (procaldecrin) was purified from porcine pancreas acetone powder. Procaldecrin showed chymotrypsin activity after trypsin treatment in a time- and dose-dependent manner. Procaldecrin did not possess serum calcium-decreasing activity but acquired serum calcium-decreasing activity as well as protease activity after trypsin treatment. However, PMSF treatment after activation of procaldecrin by trypsin did not affect the serum calcium-decreasing activity, even though protease activity was nullified by treatment with PMSF. These findings suggest that the serum calcium-decreasing activity acquired by procaldecrin requires conformational change caused by trypsin treatment.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tomomura
authors.forename: T
authors.initials: T
authors.lastname: Fukushige
authors.forename: M
authors.initials: M
authors.lastname: Tomomura
authors.forename: T
authors.initials: T
authors.lastname: Noikura
authors.forename: Y
authors.initials: Y
authors.lastname: Nishii
authors.forename: T
authors.initials: T
authors.lastname: Saheki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000097:blood*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000378:metabolism*; D013552:Swine; D014357:Trypsin / Q000378:metabolism*
chemical_list: D004792:Enzyme Precursors; D012697:Serine Endopeptidases; C074385:caldecrin; D014357:Trypsin; D002118:Calcium