title: Synthesis and distribution of rat Sertoli cell proteoglycans are modulated by linoleate and vitamin E.
abstract: In rat Sertoli cells, linoleate addition modified cell membrane fatty acid composition and changes depended on linoleate concentrations. In presence of the lowest 18:2 n-6 concentrations (2.5 and 7.5 microM), decrease in proteoglycan synthesis paralleled increase in n-6 linoleate-derived metabolites. At high concentration (21 microM), linoleate accumulated in membranes and level of n-6 linoleate-derived metabolites returned to basal value, without change in proteoglycan synthesis. Linoleate modified proteoglycan distribution in Sertoli cells by an increase in peripheral proteoglycans and a concomitant decrease in medium proteoglycans. Vitamin E (100 microM) did not alter fatty acid composition in control and linoleate-treated cells, but enhanced proteoglycan production. Furthermore, this agent counteracted linoleate-induced modifications in proteoglycan cell distribution.
authors.affiliation: Departement de Chimie, Faculté des Sciences de Meknès, Maroc.
authors.forename: H
authors.initials: H
authors.lastname: Oulhaj
authors.forename: S
authors.initials: S
authors.lastname: Huynh
authors.forename: M
authors.initials: M
authors.lastname: Langris
authors.forename: P
authors.initials: P
authors.lastname: Barbey
authors.forename: J
authors.initials: J
authors.lastname: Bocquet
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D008297:Male; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D005227:Fatty Acids; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D011509:Proteoglycans; D014810:Vitamin E; D019787:Linoleic Acid