title: Measurements of interleukin-6 in gingival crevicular fluid from adults with destructive periodontal disease.
abstract: This paper describes a study of whether or not the amounts of interleukin-6 (IL-6) in gingival crevicular fluid (GCF) are correlated with periodontal clinical measures. A sensitive ELISA was developed to measure IL-6 in GCF. Two male and 3 female adult subjects with periodontal disease were examined at their first appointments, after 3 months, and after 6 months. Data were obtained on plaque index (PI), bleeding index (BI), probing depth (PD), and on the IL-6 content of GCF samples from 16 sites per subject for a total of 240 measurements. Significant correlations were found between BI and IL-6 (P < 0.005) and between PD and IL-6 (P < 0.05), but not between PI and IL-6. Only 6 out of the 80 sites (in 3 of the 5 subjects) showed PD increases of at least 2 mm. However, for each of these 3 subjects, the amounts of IL-6 in the GCF samples from these sites were markedly higher than the mean amounts of IL-6 in the GCF samples from the remaining sites. These findings suggest that IL-6 may be a useful indicator of periodontal disease, although more extensive longitudinal studies are needed to determine the real clinical value of this GCF component.
authors.affiliation: Northwestern University Dental School, Division of Periodontics, Chicago, IL.
authors.forename: M
authors.initials: M
authors.lastname: Geivelis
authors.forename: D W
authors.initials: DW
authors.lastname: Turner
authors.forename: E D
authors.initials: ED
authors.lastname: Pederson
authors.forename: B L
authors.initials: BL
authors.lastname: Lamberts
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D015415:Biomarkers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000276:immunology*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology* / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D015850:Interleukin-6