title: Regional localization of the gene coding for the GM2 activator protein (GM2A) to chromosome 5q32-33 and confirmation of the assignment of GM2AP to chromosome 3.
abstract: The gene coding for the GM2 activator protein (GM2A) was previously mapped by us to chromosome 5 by an ELISA-based technique. Here we confirm this assignment using a PCR analysis of somatic cell hybrids and describe a regional localization to chromosome 5q32-33 by in situ hybridization. We also confirm the assignment of a pseudogene GM2AP to chromosome 3.
authors.affiliation: MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London, UK.
authors.forename: D M
authors.initials: DM
authors.lastname: Swallow
authors.forename: I
authors.initials: I
authors.lastname: Islam
authors.forename: M F
authors.initials: MF
authors.lastname: Fox
authors.forename: S
authors.initials: S
authors.lastname: Povey
authors.forename: H
authors.initials: H
authors.lastname: Klima
authors.forename: U
authors.initials: U
authors.lastname: Schepers
authors.forename: K
authors.initials: K
authors.lastname: Sandhoff
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D049289:G(M2) Activator Protein; D005678:G(M2) Ganglioside / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells; D007438:Introns; D007621:Karyotyping; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000235:genetics*; D011544:Pseudogenes; D012377:Rodentia
chemical_list: D017931:DNA Primers; D049289:G(M2) Activator Protein; D016376:Oligonucleotides, Antisense; D011506:Proteins; D005678:G(M2) Ganglioside