A single protein immunologically identified as CD38 displays NAD+ glycohydrolase, ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activities at the outer surface of human erythrocytes.
The three ectoenzyme activities, NAD+ glycohydrolase, ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase were purified to homogeneity from solubilized human erythrocyte membranes. The purification procedure involved three sequential chromatography steps on hydroxylapatite, immobilized Cu++ and immobilized anti-CD38 monoclonal antibody resins. The final step yielded a single 46 kDa protein displaying all three enzymatic activities. Since the protein bound specifically to the anti-CD38 resin, it was immunologically identified as CD38, a 46 kDa surface antigen involved in activation and proliferation of lymphocyte populations.
Zocchi
E
E
Institute of Biochemistry, University of Genoa, Italy.
Franco
L
L
Guida
L
L
Benatti
U
U
Bargellesi
A
A
Malavasi
F
F
Lee
H C
HC
De Flora
A
A
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000097:blood* / Q000302:isolation & purification; D000943:Antigens, Differentiation / Q000097:blood* / Q000302:isolation & purification; D002712:Chlorides / Q000494:pharmacology; D002845:Chromatography; D003300:Copper / Q000494:pharmacology; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008562:Membrane Glycoproteins; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000097:blood* / Q000302:isolation & purification; D009244:NAD+ Nucleosidase / Q000097:blood* / Q000302:isolation & purification; D017967:Zinc Compounds / Q000494:pharmacology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D002712:Chlorides; D008562:Membrane Glycoproteins; D017967:Zinc Compounds; D003300:Copper; C016837:zinc chloride; D017886:Durapatite; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1; C029892:cupric chloride