RNA editing of a conserved reading frame in plant mitochondria increases its similarity to two overlapping reading frames in Escherichia coli.
An open reading frame (orfx) in mitochondria of the higher plants Oenothera berteriana and Arabidopsis thaliana is homologous to orf244 in the mitochondrial genome of Marchantia polymorpha. Homologous sequences are also present in carrot, potato and sugar beet. Profile analysis revealed similarity to two overlapping reading frames in the Escherichia coli genome. Potential translation initiation at conserved ATA (isoleucine) and TTG (leucine) codons is discussed. Transcripts of the open reading frame are altered by RNA editing in Arabidopsis and Oenothera downstream of these codons, suggesting this to be the functionally important region.
Sünkel
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Institut für Genbiologische Forschung, Berlin, Germany.
Brennicke
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Knoop
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D000595:Amino Acid Sequence; D017360:Arabidopsis; D001483:Base Sequence; D017124:Conserved Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D010442:Peptide Chain Initiation, Translational; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants; D017393:RNA Editing; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004272:DNA, Mitochondrial; D015345:Oligonucleotide Probes; D010940:Plant Proteins