Autoradiographic localization of strychnine-sensitive glycine receptor in bovine adrenal medulla.
The presence of an uptake system and a functional glycine receptor in adrenal medulla chromaffin cells was investigated using an autoradiographic technique in adrenal gland slices. Specific 3[H]glycine binding was observed in both adrenal cortex and medulla slices, while only specific binding of [3H]strychnine was seen only in chromaffin cells and was not associated with cortical cells. [3H]Glycine binding sites in the cortex are apparently different from those of [3H]strychnine binding sites in the medulla since excess strychnine does not displace [3H]glycine from adrenal cortex but does so from medulla. This difference supports biochemical evidence for glycine transport into medulla cells and glycine receptor sites on the chromaffin cell membrane.
Yadid
G
G
Department of Pharmacology, Technion-Bruce Rappaport Faculty of Medicine, Haifa, Israel.
Maor
G
G
Youdim
M B
MB
Silberman
M
M
Zinder
O
O
D000302:Adrenal Cortex / Q000737:chemistry* / Q000378:metabolism; D000313:Adrenal Medulla / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002838:Chromaffin System / Q000737:chemistry / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D018009:Receptors, Glycine / Q000032:analysis* / Q000378:metabolism; D013331:Strychnine / Q000378:metabolism / Q000494:pharmacology*; D014018:Tissue Distribution; D014316:Tritium
D018009:Receptors, Glycine; D014316:Tritium; D013331:Strychnine; D005998:Glycine