title: Comparison of the cross-linking characteristics of porcine heart valves fixed with glutaraldehyde or epoxy compounds.
abstract: The concerns about currently available bioprosthetic heart valves are calcification, long-term durability, and functional and hemodynamic performance. These concerns are all more or less related to the cross-linking reagents, glutaraldehyde or formaldehyde, used in fixing bioprostheses. To address these concerns, the authors undertook the development of a porcine heart valve cross-linked with an epoxy compound. This study compared the cross-linking characteristics, shrink temperature, and moisture content of porcine heart valves fixed with epoxy compounds or glutaraldehyde. Two types of epoxy compounds, Denacol EX-313 and EX-810, or a 0.625% glutaraldehyde were used to fix the porcine aortic valves procured from a slaughter house. Samples of each group were removed at various elapsed fixation times. The shrink temperature and moisture content of the valvular leaflet and distinct layers of aortic wall of each sample were measured. Fresh porcine aortic valve was used as a control. It was found that the shrink temperature of the glutaraldehyde leaflet was the highest, whereas the moisture content of the EX-313 leaflet was the greatest among the three test groups. No significant difference in shrink temperature was observed among the epoxy compound fixed inner, middle, outer, and entire aortic walls. This implied that the cross-linking density of the epoxy compound valve was uniform throughout the entire aortic wall. The same also was observed for the glutaraldehyde fixed aortic wall.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Baxter Edwards Cardiovascular Surgery Division, Irvine, CA 92714-5686.
authors.forename: H W
authors.initials: HW
authors.lastname: Sung
authors.forename: S H
authors.initials: SH
authors.lastname: Shen
authors.forename: R
authors.initials: R
authors.lastname: Tu
authors.forename: D
authors.initials: D
authors.lastname: Lin
authors.forename: C
authors.initials: C
authors.lastname: Hata
authors.forename: Y
authors.initials: Y
authors.lastname: Noishiki
authors.forename: Y
authors.initials: Y
authors.lastname: Tomizawa
authors.forename: R C
authors.initials: RC
authors.lastname: Quijano
mesh_terms: D000818:Animals; D001021:Aortic Valve; D001705:Bioprosthesis; D003432:Cross-Linking Reagents; D004852:Epoxy Compounds; D005976:Glutaral; D006350:Heart Valve Prosthesis; D011474:Prosthesis Design; D013552:Swine; D016707:Tissue Fixation
chemical_list: D003432:Cross-Linking Reagents; D004852:Epoxy Compounds; D005976:Glutaral