Promotion of fetal liver cell engraftment in irradiated mice by activated T lymphocytes.
The promoting effect of T lymphocytes on hematopoietic cell engraftment was studied in lethally irradiated CBA and Balb/c mice by transplantation of either liver cells from Balb/c fetuses (FLBalb) or FLBalb plus Balb/c T lymphocytes (TBalb). Additional groups of lethally irradiated CBA and Balb/c mice received T lymphocytes only, either from CBA (TCBA) or from Balb/c donors. Injection of TBalb lymphocytes had no effect on the splenic indices (SI) nor on the numbers of colony-forming units in the spleens (CFU-S) of FLBalb-transplanted Balb/c mice on day 7. In contrast, it increased these parameters in the CBA recipients of FLBalb in a T cell-dose dependent fashion and it stimulated their hematologic recovery on day 20 following irradiation. TCBA and TBalb lymphocytes transplanted alone did not induce CFU-S in syngeneic recipients but promoted the development of CFU-S of endogenous origin in the histoincompatible mice. The development of CFU-S was enhanced in the FLBalb-transplanted Balb/c mice by injection of TBalb lymphocytes previously stimulated in vitro with irradiated CBA splenocytes, phorbol myristate acetate (PMA), phytohemagglutinin (PHA) or concanavalin A (conA). This effect was associated with an increased survival rate of the recipient mice. Daily injections for 6 days of a culture supernatant from a mixture of TBalb cells and irradiated CBA splenocytes, but not from irradiated CBA splenocytes alone, promoted the development of CFU-S in FLBalb-transplanted Balb/c recipients. However, the supernatant of stimulated TBalb cells was less efficient than the stimulated T cells themselves.(ABSTRACT TRUNCATED AT 250 WORDS)
Plotnicky
H
H
Unité de recherches en Néphro-Urologie de Transplantation et Immunologie Clinique, Hôpital Edouard Herriot, Lyon, France.
Touraine
J L
JL
D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D016332:Fetal Tissue Transplantation; D006082:Graft Enhancement, Immunologic; D006085:Graft Survival / Q000187:drug effects; D006086:Graft vs Host Disease / Q000517:prevention & control; D006648:Histocompatibility; D008099:Liver / Q000166:cytology* / Q000196:embryology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011828:Radiation Chimera; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*
D017077:Culture Media, Conditioned; D008222:Lymphokines