title: Chronic exposure to lidocaine does not alter flux through sodium channels in cultured neuronal cells.
abstract: OBJECTIVE
  Although tachyphylaxis to local anesthetics has been reported in the clinical literature for more than two decades, the molecular mechanism(s) remain unknown. The authors described an attempt to create an in vitro model for tachyphylaxis to local anesthetics using cultured neuronal cells.
  
  
  METHODS
  Murine neuroblastoma cells (N1E115) and rat pheochromocytoma cells (PC-12) were grown in the presence or absence of lidocaine or tetrodotoxin for between 1 and 14 days. Thereafter, the authors tested flux through sodium channels by measuring total and tetrodotoxin-sensitive flux of 14C-labeled guanidinium (a ligand for the sodium channel) into the cells using the technique of Jacques et al.
  
  
  RESULTS
  Chronic lidocaine or tetrodotoxin treatment caused no change relative to control cells in total or tetrodotoxin-sensitive guanidinium flux, or in the subsequent ability of lidocaine in the flux assay mixture to inhibit guanidinium flux.
  
  
  CONCLUSIONS
  The authors concluded that chronic lidocaine or tetrodotoxin application did not produce changes in stimulated sodium channel activity or subsequent lidocaine susceptibility in this model. To the extent that this model simulated the clinical situation, mechanisms other than up-regulation of sodium channel number or maximal stimulated flux per channel may have been responsible for producing tachyphylaxis.
authors.affiliation: Children's Hospital, Boston 02115.
authors.forename: R T
authors.initials: RT
authors.lastname: Wilder
authors.forename: C B
authors.initials: CB
authors.lastname: Berde
authors.forename: C T
authors.initials: CT
authors.lastname: Griggs
authors.forename: R
authors.initials: R
authors.lastname: Chickalingaiah
authors.forename: G
authors.initials: G
authors.lastname: Strichartz
mesh_terms: D000818:Animals; D002452:Cell Count / Q000187:drug effects; D019791:Guanidine; D006146:Guanidines / Q000378:metabolism; D008012:Lidocaine / Q000494:pharmacology*; D051379:Mice; D016501:Neurites / Q000187:drug effects; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D015222:Sodium Channels / Q000187:drug effects* / Q000378:metabolism; D013618:Tachyphylaxis / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014701:Veratridine / Q000494:pharmacology
chemical_list: D006146:Guanidines; D015222:Sodium Channels; D014701:Veratridine; D008012:Lidocaine; D019791:Guanidine