title: Isolation and characterization of 14 CA-repeat microsatellites from human chromosome 21.
abstract: Fourteen CA-repeat microsatellite polymorphisms from human chromosome 21 (HC21) (D21S364, D21S365, D21S366, D21S367, D21S368, D21S369, D21S406, D21S407, D21S408, D21S409, D21S410, D21S411, D21S415, and D21S1217) have been cloned from a flow-sorted phage library (LA21NS01). These microsatellite loci have been mapped using a somatic cell hybrid panel of HC21, and heterozygosities and allele frequencies have been determined. The markers spread along the length of the long arm of the HC21. Twelve of these microsatellites have heterozygosities between 0.47 and 0.90. These new CA-repeat markers will improve the genetic map of HC21.
authors.affiliation: Molecular Genetics Department, Hospital Duran i Reynals, Barcelona, Catalunya, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Bosch
authors.forename: V
authors.initials: V
authors.lastname: Nunes
authors.forename: D
authors.initials: D
authors.lastname: Patterson
authors.forename: X
authors.initials: X
authors.lastname: Estivill
mesh_terms: D000818:Animals; D001483:Base Sequence; D002891:Chromosomes, Human, Pair 21; D006224:Cricetinae; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D017931:DNA Primers; D004276:DNA, Satellite