title: The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member.
abstract: Certain plant phenolic compounds and monosaccharides induce the transcription of virulence (vir) genes of Agrobacterium tumefaciens through the VirA-VirG two-component regulatory system. The product of the chromosomal virulence gene chvE is homologous to galactose-binding protein of Escherichia coli and is required for vir gene induction by sugars. Adjacent to, but divergent in transcription from, chvE is an open reading frame, now termed gbpR (galactose-binding protein regulator), that is homologous to the LysR family of transcriptional regulators. chvE::lacZ expression was induced by L-arabinose, D-galactose, and D-fucose when gbpR was present. In the absence of inducer, GbpR repressed chvE::lacZ expression. In addition, GbpR negatively regulated its own expression.
authors.affiliation: Department of Microbiology, University of Washington, Seattle 98195.
authors.forename: S L
authors.initials: SL
authors.lastname: Doty
authors.forename: M
authors.initials: M
authors.lastname: Chang
authors.forename: E W
authors.initials: EW
authors.lastname: Nester
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000472:pathogenicity*; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002241:Carbohydrates / Q000494:pharmacology; D002352:Carrier Proteins / Q000096:biosynthesis; D002876:Chromosomes, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation; D014774:Virulence / Q000235:genetics*; D037521:Virulence Factors; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D002241:Carbohydrates; D002352:Carrier Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D037521:Virulence Factors; C023550:galactose-binding protein; C058483:virG protein, Shigella flexneri; C073470:LysR protein, Bacteria; D001616:beta-Galactosidase