Compositional compartmentalization of the nuclear genomes of Trypanosoma brucei and Trypanosoma equiperdum.
High molecular weight DNA preparations from Trypanosoma brucei and Trypanosoma equiperdum were fractionated by preparative centrifugation in a Cs2SO4 density gradient in the presence of BAMD, bis(acetatomercurimethyl)dioxane, a sequence-specific DNA ligand. Analytical centrifugation in CsCl of the DNA fractions so obtained showed that both DNAs had a bimodal distribution with two major peaks banding at 1.702-1.703 and 1.708 g/cm3 and representing 1/3 and 2/3 of total DNA, respectively. Several minor components were also detected. These results indicate that a compositional compartmentalization is not only found in the genome of vertebrates and plants, as already described, but also in those of protozoa such as Trypanosomes.
Isacchi
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A
Laboratoire de Génétique Moléculaire, Institut Jacques Monod, Paris, France.
Bernardi
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Bernardi
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D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry*; D002499:Centrifugation, Density Gradient; D016054:DNA, Protozoan / Q000302:isolation & purification; D016678:Genome; D008767:Methylmercury Compounds; D014345:Trypanosoma / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics*
D016054:DNA, Protozoan; D008767:Methylmercury Compounds; C006759:3,6-bis(acetatomercurimethyl)dioxane