12-Lipoxygenase product as an inhibitor of the action of chemoattractant peptide fMet-Leu-Phe in rat neutrophils.
1. 12-Hydroxyeicosatetraenoic acid (12-HETE) has been evaluated for its capacities to modulate neutrophil migration and cytosolic Ca2+ ([Ca2+]i) using compounds prepared by chemical synthesis and tissue extract from dog gingiva. 2. 12-HETE inhibited N-formyl-Met-Leu-Phe (fMLP)-stimulated neutrophil migration in a concentration-dependent fashion. 3. The tissue extract from dog gingiva mimicked the actions of 12-HETE. 4. Although 12-HETE failed to increase [Ca2+]i, preincubation of neutrophils with 12-HETE led to a suppression of [Ca2+]i when the cells were subsequently stimulated by fMLP. 5. Again tissue extract from dog gingiva mimicked the action of 12-HETE on [Ca2+]i. 6. These results suggest the possible correlation of the inhibitory activities of 12-HETE on the regulation of neutrophil migration and Ca2+ mobilization, and this may be important for the role of 12-HETE in pathogenesis in periodontal tissues.
Yoshino
H
H
Department of Periodontics, Hiroshima University School of Dentistry, Japan.
Kitayama
S
S
Morita
K
K
Okamoto
H
H
Tsujimoto
A
A
Dohi
T
T
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D005881:Gingiva / Q000502:physiology; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014020:Tissue Extracts / Q000494:pharmacology
D006893:Hydroxyeicosatetraenoic Acids; D014020:Tissue Extracts; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D019204:GTP-Binding Proteins; D002118:Calcium