Glycerophosphorylcholine phosphocholine phosphodiesterase activity during the differentiation of glial progenitor cells.
O-2A progenitor cells were grown in medium containing either 1% or 10% fetal calf serum (FCS) for 4 weeks. The cells in 1% FCS were 75% oligodendrocytes by 3 weeks in culture. The cell population was so overgrown with astrocytes in the 10% medium that an accurate estimate of cell number could not be made. The activities of glycerophosphorylcholine phosphocholine phosphodiesterase (GPC-PC-PdE), p-nitrophenylphosphorylcholine phosphodiesterase (pNPPC-PC-PdE), and ceramide UDP galactose galactosyl transferase (CGalT) were barely detectable in the cells grown in 10% FCS. The activities of these 3 enzymes were low in the cells grown in 1% FCS for the first 2 weeks and then all 3 increased manyfold. These observations reinforce the evidence previously accrued showing that these two phosphodiesterase activities (GPC-PC-PdE and pNPPC-PC-PdE) are markers of oligodendroglial cells as well as myelin. In contrast, glycerophosphorylcholine choline phosphodiesterase (GPC-C-PdE) activities were present in cells grown in both 1% and 10% FCS.
Monge
M
M
INSERM U 134, Université P., et M. Curie, Hôpital de la Salpêtrière, Paris, France.
Yuan
J
J
Cabon
F
F
Zalc
B
B
Kanfer
J N
JN
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000201:enzymology; D001921:Brain / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D017628:Microglia / Q000166:cytology / Q000201:enzymology; D009457:Neuroglia / Q000166:cytology* / Q000201:enzymology; D009836:Oligodendroglia / Q000166:cytology / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology
D005904:Glial Fibrillary Acidic Protein; D010727:Phosphoric Diester Hydrolases; C061931:glycerophosphocholine cholinephosphodiesterase