title: Reduced bacterial adhesion to heparin-surface-modified intraocular lenses.
abstract: Bacterial adherence to intraocular lenses (IOLs) could be the cause of endophthalmitis following cataract surgery and lens implantation. There are previous reports that heparin bound to the surface of poly(methyl methacrylate) (PMMA) IOLs reduces cell adhesion. In this study, the in vitro adherence of Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa to regular PMMA IOLs and to heparin-surface-modified (HSM) PMMA IOLs was investigated. The three bacterial strains attached in significantly lower numbers to HSM-PMMA IOLs than to PMMA IOLs (P < .01). Heparin in solution also inhibited attachment of Staphylococcus epidermidis to regular PMMA IOLs. Heparin may reduce adherence by placing a highly hydrated layer between the bacteria and the IOL surface. Therefore, the use of HSM-PMMA IOLs could diminish the incidence of endophthalmitis.
authors.affiliation: Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114.
authors.forename: M
authors.initials: M
authors.lastname: Portolés
authors.forename: M F
authors.initials: MF
authors.lastname: Refojo
authors.forename: F L
authors.initials: FL
authors.lastname: Leong
mesh_terms: D001422:Bacterial Adhesion; D015169:Colony Count, Microbial; D009877:Endophthalmitis / Q000517:prevention & control; D006493:Heparin; D007910:Lenses, Intraocular / Q000009:adverse effects; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D008855:Microscopy, Electron, Scanning; D011550:Pseudomonas aeruginosa / Q000502:physiology* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000502:physiology* / Q000648:ultrastructure; D013212:Staphylococcus epidermidis / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D008768:Methylmethacrylates; D020366:Methylmethacrylate; D006493:Heparin