title: Effects of hydrocortisone on the lymphoid organs of Rana temporaria.
abstract: In vivo and in vitro effects of hydrocortisone hemisuccinate (HC) on the lymphoid organs were studied in the European common frog Rana temporaria. Single HC injection at the dose of 0.5 mg/g body weight induced rapid but fully reversible changes in thymuses and jugular bodies while its effect on spleens was negligible. In vitro studies confirmed HC-sensitivity of thymuses and jugular bodies and relative HC-resistance of spleens of this species. In vitro exposition of thymic and jugular body cell suspensions to 0.25-1.00 mg/ml HC solution revealed a dose-dependent and time-dependent impairment of sample activities associated with massive cell death as judged by MTT reduction assay and Trypan blue uptake, respectively. Evolutionary implications of these results are discussed.
authors.affiliation: Laboratory of Evolutionary Immunology, Jagiellonian University, Kraków, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Płytycz
authors.forename: E
authors.initials: E
authors.lastname: Kusina
authors.forename: A
authors.initials: A
authors.lastname: Józkowicz
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006854:Hydrocortisone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D008221:Lymphoid Tissue / Q000187:drug effects*; D008297:Male; D010084:Oxidation-Reduction; D011896:Rana temporaria; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: C007133:hydrocortisone hemisuccinate; D006854:Hydrocortisone