Volume-regulatory taurine release from a human lung cancer cell line. Evidence for amino acid transport via a volume-activated chloride channel.
Exposure of a human lung epithelial cancer cell-line to hypo-osmotic media led to a marked increase in the rate of efflux from the cells of taurine, a non-essential sulfonic amino acid. The osmotically-activated taurine efflux was inhibited by a range of known Cl- channel blockers, the most potent of which were NPPB and 1,9-dideoxyforskolin. These reagents were similarly effective at inhibiting the osmotically-activated efflux of I-, a known substrate of volume-activated Cl- channels. The results are consistent with the hypothesis that volume-regulatory taurine release from these cells is mediated by a volume-activated Cl- channel.
Kirk
K
K
University Laboratory of Physiology, UK.
Kirk
J
J
D000596:Amino Acids / Q000378:metabolism*; D001692:Biological Transport; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005576:Colforsin / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007454:Iodides / Q000378:metabolism; D008175:Lung Neoplasms; D009579:Nitrobenzoates / Q000494:pharmacology; D009994:Osmolar Concentration; D012964:Sodium / Q000378:metabolism; D013654:Taurine / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D018118:Chloride Channels; D007454:Iodides; D009579:Nitrobenzoates; D013654:Taurine; D005576:Colforsin; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D012964:Sodium; C047983:1,9-dideoxyforskolin