title: Deoxyribonucleic acid histogram of testes in primary transsexualism.
abstract: The influence of oestrogen on spermatogenesis was evaluated by comparing its effect on the testes of primary male transsexuals and those who had undergone hormone therapy for prostatic carcinoma. Eight primary transsexuals were studied. They had been diagnosed on clinical and psychiatric evidence and had been on oestrogen therapy for several years since puberty. Histological sections of testicular tissue obtained at reassignment surgery from 8 phenotypic male transsexuals (aged 24-32 years) with an XY chromosome complement were studied by light microscopy. The formalin-embedded specimens were analysed by flow cytometry for deoxyribonucleic acid (DNA) histograms. Both the histology and DNA histograms revealed a pattern of maturation arrest in 12 of 16 testes in which the diploid cell compartment occupied most of the spermatogenetic element, followed by tetraploid and monoploid cells. Two testes showed impaired spermatogenesis and 2 were normal. The DNA histograms and pathology were also evaluated in 20 testes after 3 to 8 years of hormone therapy in patients with advanced prostatic carcinoma (aged 60-78 years). No maturation arrest was found in these patients. Sixteen of them had a pattern of fibrosis and atrophy and 4 had impaired spermatogenesis. It was concluded that oestrogen influenced spermatogenesis and affected the maturation of spermatogonia mostly during puberty.
authors.affiliation: Division of Urology, National Yang-Ming Medical College, Veterans General Hospital-Taipei, Taiwan, Republic of China.
authors.forename: A W
authors.initials: AW
authors.lastname: Chiu
authors.forename: M T
authors.initials: MT
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Chiang
authors.forename: L H
authors.initials: LH
authors.lastname: Wu
authors.forename: R H
authors.initials: RH
authors.lastname: Fang
authors.forename: L S
authors.initials: LS
authors.lastname: Chang
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D004247:DNA / Q000032:analysis*; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000473:pathology; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D014189:Transsexualism / Q000235:genetics / Q000473:pathology*
chemical_list: D004967:Estrogens; D004247:DNA