A model for cleavage of O-glycosidic bonds in glycoproteins.
The present work investigated the possibility of cleavage of alpha-linkages between mannose or galactose and serine/threonine residues by alpha-mannosidase and alpha-galactosidase. The study was carried out initially with model synthetic compounds imitating the O-glycosidic bond in glycoproteins, and further with glucoamylase. It was shown that alpha-mannosidase and alpha-galactosidase can hydrolyse these linkages after proteolytic digestion of glucoamylase.
Ibatullin
F M
FM
Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Gatchina, Russia.
Golubev
A M
AM
Firsov
L M
LM
Neustroev
K N
KN
D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D006031:Glycosylation; D006868:Hydrolysis; D007700:Kinetics; D008361:Mannosidases / Q000378:metabolism; D000519:alpha-Galactosidase / Q000378:metabolism; D043323:alpha-Mannosidase
D006023:Glycoproteins; D006027:Glycosides; D008361:Mannosidases; D000519:alpha-Galactosidase; D043323:alpha-Mannosidase; D005087:Glucan 1,4-alpha-Glucosidase