title: Heart muscle cells acutely infected with Trypanosoma cruzi: characterization of electrophysiology and neurotransmitter responses.
abstract: Primary cell cultures of mouse ventricular myocardium were infected with Trypanosoma cruzi, to study the consequences of T. cruzi-muscle cell interaction on the rate of spontaneous contractions, on the responses to norepinephrine, and on action potential parameters. Single cells or small cell groups of infected cultures were subjected to pharmacological and electrophysiological experiments. In concentrations ranging from 1 nM to 100 microM, norepinephrine exerted positive or negative chronotropic effects mediated by alpha-adrenergic receptors. A significant number of infected cells (25%) did not respond to the agonist. Two days after infection the cultures exhibited a higher frequency of spontaneous contractions (20%), paralleled by an increase in firing rate and a decrease in the action potential duration without significant changes in maximum diastolic potential and action potential amplitude. A decrease in alpha-adrenergic receptor-mediated positive chronotropic response to norepinephrine was also observed in 2-day infected cells. Cells made to phagocyte ferritin particles showed an increase in the rate of spontaneous contractions, but no changes in the positive chronotropic responses to norepinephrine. In conclusion, these observations show that during acute infection with T. cruzi, there are alterations in automaticity and in the chronotropic responses to norepinephrine, whose mechanisms are related to the process of parasite endocytosis by the cardiac cells.
authors.affiliation: Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Faneiro, Brasil.
authors.forename: O
authors.initials: O
authors.lastname: Aprigliano
authors.forename: M O
authors.initials: MO
authors.lastname: Masuda
authors.forename: M N
authors.initials: MN
authors.lastname: Meirelles
authors.forename: M C
authors.initials: MC
authors.lastname: Pereira
authors.forename: H S
authors.initials: HS
authors.lastname: Barbosa
authors.forename: J C
authors.initials: JC
authors.lastname: Barbosa
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002598:Chagas Cardiomyopathy / Q000503:physiopathology*; D004594:Electrophysiology; D006321:Heart / Q000503:physiopathology*; D006339:Heart Rate / Q000187:drug effects; D051379:Mice; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology; D009638:Norepinephrine / Q000494:pharmacology*; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology
chemical_list: D011433:Propranolol; D009638:Norepinephrine; D010646:Phentolamine