Replication of adenovirus DNA in vitro is ATP-independent.
Using a reconstituted system for adenovirus DNA replication we tested the requirements for ATP and divalent cations. At the standard Mg2+ concentration ATP stimulated initiation 5 to 10-fold. However, this effect was caused by complexing Mg2+. At the optimal Mg2+ concentration ATP was not required for initiation or elongation. Besides Mg2+ also Mn2+, Ca2+ and Ba2+ were shown to support initiation whereas for elongation only Mg2+ was accepted. Since Mn2+ could efficiently be used for DNA chain elongation on synthetic templates we hypothesize that Mg2+ is essential for the transition of initiation to elongation.
Pronk
R
R
Laboratory for Physiological Chemistry, Utrecht University, The Netherlands.
Van Driel
W
W
Van der Vliet
P C
PC
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D014779:Virus Replication
D002413:Cations, Divalent; D004279:DNA, Viral; D001464:Barium; D008345:Manganese; D000255:Adenosine Triphosphate; D004259:DNA-Directed DNA Polymerase; D008274:Magnesium; D002118:Calcium