Amelogenin post-translational modifications: carboxy-terminal processing and the phosphorylation of bovine and porcine "TRAP" and "LRAP" amelogenins.
TRAP and LRAP amelogenin components were isolated by size-exclusion and reversed-phase HPLC from developing dental enamel. Porcine developing enamel contains TRAP and LRAP components analogous to those of bovine. Amino acid composition and mass spectrographic analyses established that, in both species, the carboxy-terminal sequences of the LRAP components are two residues longer than previously reported for bovine LRAP, and that both the TRAPs and LRAPs contained a single phosphorylated residue. These amelogenin polypeptides were the principal components of the enamel protein lower molecular weight fraction. The LRAP sequence data for both species suggests that the mechanism of amelogenin carboxy-terminal processing may differ significantly from that previously suggested.
Fincham
A G
AG
University of Southern California, Center for Craniofacial Molecular Biology, Los Angeles 90033.
Moradian-Oldak
J
J
D053523:Amelogenin; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003743:Dental Enamel / Q000378:metabolism; D003746:Dental Enamel Proteins / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010766:Phosphorylation; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013552:Swine; D014083:Tooth Germ / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D053523:Amelogenin; D000596:Amino Acids; D003746:Dental Enamel Proteins; D011498:Protein Precursors; C067432:leucine-rich amelogenin peptide; C084495:tyrosine-rich amelogenin polypeptide; D014443:Tyrosine; D007930:Leucine