Bovine and porcine large intestine as model epithelia in a student lab course.
A short-circuit current experiment on epithelial ion transport is described that is suitable for student classes in human and animal physiology. Segments of late distal colon from either pig or cow are obtained from the slaughterhouse depending on the animals' daily schedule. Initial tissue preparation already in the slaughterhouse, cold storage, and proper choice of bath solutions are essential prerequisites for success. Students monitor spontaneous transepithelial voltage and short-circuit current (Isc) by use of manually operated voltage clamp units. Two main transport mechanisms are studied, electrogenic Na+ absorption and Cl- secretion. Electrogenic Na+ absorption is studied by measuring the Isc drop after amiloride. Then Cl- secretion is stimulated by theophylline and subsequently inhibited by furosemide. In some experiments K+ secretion can be detected by the blocking effect of mucosal Ba2+. Response of tissues from pig and cow is qualitatively similar but quantitatively different. The equipment is sturdy and inexpensive, can be provided by most departmental workshops, and has been tested for 3 yr in regular lab courses. Observations made during these experiments are closely related to clinical states, such as secretory diarrhea, cystic fibrosis, and hyperaldosteronism, as well as to the mechanisms of clinically used diuretics.
Hegel
U
U
Institut für Klinische Physiologie, Klinikum Steglitz der Freien Universität Berlin, Germany.
Fromm
M
M
Kreusel
K M
KM
Wiederholt
M
M
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D002712:Chlorides / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D007420:Intestine, Large / Q000187:drug effects / Q000502:physiology*; D010827:Physiology / Q000193:education* / Q000295:instrumentation; D012964:Sodium / Q000378:metabolism; D013552:Swine; D013663:Teaching / Q000379:methods; D014021:Tissue Preservation
D002712:Chlorides; D012964:Sodium