Cell vacuolization induced by Helicobacter pylori: inhibition by bafilomycins A1, B1, C1 and D.
All available bafilomycins (A1, B1, C1 and D) inhibit and revert macroscopic vacuolization induced by Helicobacter pylori cell-free extracts. Bafilomycin A1 displays the highest activity, followed by bafilomycin B1, C1 and D. The different potency of bafilomycins correlates with their ability to inhibit the vacuolar-type ATPase (V-ATPase) and to dissipate the membrane pH gradient of intracellular acidic organelles. These results suggest that bafilomycins should be considered as possible therapeutic agents in the treatment of gastritis.
Papini
E
E
Dipartimento di Scienze Biomediche, Università di Padova, Italy.
de Bernard
M
M
Bugnoli
M
M
Milia
E
E
Rappuoli
R
R
Montecucco
C
C
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000187:drug effects* / Q000472:pathogenicity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000201:enzymology; D018942:Macrolides; D015394:Molecular Structure; D051381:Rats; D014617:Vacuoles / Q000201:enzymology
D000900:Anti-Bacterial Agents; D018942:Macrolides; D000251:Adenosine Triphosphatases