Influence of dietary aluminum on cytokine production by mitogen-stimulated spleen cells from Swiss Webster mice.
Swiss Webster mice were exposed to excess dietary aluminum (Al) (1000 micrograms Al/g diet, Al as Al lactate) from conception to 6 months of age. Splenic lymphocytes (10(6) per culture) were incubated for 24 hrs with concanavalin A (5 micrograms/ml). Concentrations of interleukin-2, interferon-gamma and tumor necrosis factor-alpha, as measured in supernatants via ELISA with monoclonal antibodies, were depressed in spleen cells from aluminum treated mice relative to controls. Experiments using the fluorescence activated cell sorter demonstrated a shift in T-cell populations from treated mice with a deficiency of CD4+ cells. These findings suggest a deficit in immune effector cell function after long term in vivo aluminum exposure.
Golub
M S
MS
Department of Internal Medicine, School of Medicine, University of California, Davis 95616.
Takeuchi
P T
PT
Gershwin
M E
ME
Yoshida
S H
SH
D000535:Aluminum / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D004032:Diet / Q000009:adverse effects; D005260:Female; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000633:toxicity; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008431:Maternal-Fetal Exchange; D051379:Mice; D011247:Pregnancy; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D000535:Aluminum