Ferric reductases in Escherichia coli: the contribution of the haemoglobin-like protein.
The haemoglobin-like protein (HMP) of E. coli previously isolated as a dihydropteridine reductase was shown to be also a ferric citrate reductase. We demonstrate that, in fact, HMP is a flavin reductase and that its ferric reductase activity is a result of its ability to reduce free flavins. However, when compared to the two main ferric/flavin reductases of E. coli, i.e., the NAD(P)H: flavin oxidoreductase and the sulfite reductase, one can conclude that the contribution of HMP to iron reduction is negligible.
Eschenbrenner
M
M
Laboratoire d'Etudes Dynamiques et Structurales de la Sélectivité, Université Joseph Fourier, Grenoble, France.
Coves
J
J
Fontecave
M
M
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004093:Dihydropteridine Reductase; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D038181:FMN Reductase; D005486:Flavin Mononucleotide / Q000378:metabolism; D005415:Flavins / Q000378:metabolism; D006420:Hemeproteins / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005415:Flavins; D006420:Hemeproteins; D005486:Flavin Mononucleotide; D004093:Dihydropteridine Reductase; C076538:hmp protein, E coli; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; C023903:ferric citrate iron reductase