title: Failure of sister chromatid separation leads to formation of diplochromosomes in colcemid treated PtK1 cells.
abstract: The origin of diplochromosomes has been traced in multinucleate rat kangaroo cells (PtK1) obtained after colcemid treatment. In these cells the diplochromosomes were shown to originate from restitution nuclei, indicating that they were formed due to the omission or failure of sister chromatid separation and not due to endoreduplication. In this context the mechanism of sister chromatid separation has been discussed. The independence of this mitotic event from other associated processes, such as chromosome condensation, nuclear envelope breakdown or spindle formation has been stressed.
authors.affiliation: Research Program IV, German Cancer Research Center, Heidelberg.
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.forename: N
authors.initials: N
authors.lastname: Paweletz
authors.forename: D
authors.initials: D
authors.lastname: Schroeter
mesh_terms: D000818:Animals; D002460:Cell Line; D002842:Chromatids / Q000187:drug effects / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects* / Q000648:ultrastructure; D003703:Demecolcine / Q000494:pharmacology*; D007614:Macropodidae; D008938:Mitosis; D009630:Nondisjunction, Genetic
chemical_list: D003703:Demecolcine