title: Comparative potency of broad-band and narrow-band phototherapy sources to induce edema, sunburn cells and urocanic acid photoisomerization in hairless mouse skin.
abstract: The Philips TL01 narrow-band (311-313 nm) fluorescent lamp provides effective phototherapy for psoriasis and atopic eczema while emitting less erythemogenic radiation than conventional broad-band (e.g. Philips TL12; 270-350 nm) sources. We studied the potency of TL01 and TL12 radiation to induce edema and sunburn cells (SBC) and to photoisomerize naturally occurring trans-urocanic acid (UCA) to cis-UCA in hairless mouse skin. Cis-UCA has immunosuppressive properties and is a putative mediator of UV-induced suppression of immune responses. For each source, there was UV dose dependence for all three responses. Within the dose ranges used, the potency ratio of TL12:TL01 radiation to induce equivalent edema and SBC was about 6:1. However, the potency ratio to induce cis-UCA was less than 2.3:1. Therefore, at a given level of edema or SBC induction, TL01 was more efficient than TL12 at UCA photoisomerization. The TL01 induction of immunomodulating cis-UCA, while causing minimal skin injury, may relate to the therapeutic efficacy of this source in skin conditions with an immunological component.
authors.affiliation: Photobiology Unit, Ninewells Hospital, University of Dundee, UK.
authors.forename: N K
authors.initials: NK
authors.lastname: Gibbs
authors.forename: M
authors.initials: M
authors.lastname: Norval
authors.forename: N J
authors.initials: NJ
authors.lastname: Traynor
authors.forename: J C
authors.initials: JC
authors.lastname: Crosby
authors.forename: G
authors.initials: G
authors.lastname: Lowe
authors.forename: B E
authors.initials: BE
authors.lastname: Johnson
mesh_terms: D000417:Albinism; D000818:Animals; D001415:Back / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004423:Ear / Q000528:radiation effects; D004487:Edema; D005260:Female; D007536:Isomerism; D051379:Mice; D008812:Mice, Hairless; D010789:Phototherapy / Q000295:instrumentation*; D012867:Skin / Q000166:cytology / Q000528:radiation effects*; D013471:Sunburn; D014466:Ultraviolet Rays; D014560:Urocanic Acid / Q000737:chemistry*
chemical_list: D014560:Urocanic Acid