Metabolism of benzene and trans,trans-muconaldehyde in the isolated perfused rat liver.
Perfusate from rat livers perfused with benzene (approximately 0.7-7 x 10(-4) M) or trans,trans-muconaldehyde (MUC) (10(-4) M) was extracted and analyzed by reverse-phase HPLC. Based on retention time and co-elution experiments, benzene was found to be metabolized to trans,trans-muconic acid, a urinary ring-opened metabolite of benzene and a major in vivo and in vitro metabolite of MUC. These data demonstrate that benzene ring-opening occurs in the liver. Following perfusion with MUC (a microsomal hematotoxic metabolite of benzene), trans,trans-muconic acid and three other MUC metabolites were detected in the perfusate extract, suggesting that these metabolites would be present in the circulation following metabolism of MUC.
Grotz
V L
VL
Joint Graduate Program in Toxicology, Rutgers University/UMDNJ-Robert Wood Johnson Medical School, Piscataway.
Ji
S
S
Kline
S A
SA
Goldstein
B D
BD
Witz
G
G
D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D001554:Benzene / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000378:metabolism*; D008297:Male; D009924:Organ Culture Techniques; D010477:Perfusion; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism
D000447:Aldehydes; C034476:muconaldehyde; C005939:muconic acid; D001554:Benzene; D013011:Sorbic Acid