Association of bluetongue virus gene segment 5 with neuroinvasiveness.
Two strains (UC-2 and UC-8) of bluetongue virus were used to determine genetic factors influencing neuroinvasiveness. Reassortants were produced in vitro, and the parental origins of their genes were determined by polyacrylamide gel electrophoresis profiles and restriction endonuclease digestion. Gene segment 5 of UC-8 correlated with neuroinvasiveness of reassortants when inoculated subcutaneously into newborn mice.
Carr
M A
MA
Department of Veterinary Pathology, University of California, Davis 95616.
de Mattos
C C
CC
de Mattos
C A
CA
Osburn
B I
BI
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D001819:Bluetongue / Q000382:microbiology*; D001820:Bluetongue virus / Q000235:genetics* / Q000472:pathogenicity*; D001921:Brain / Q000382:microbiology*; D002417:Cattle; D005814:Genes, Viral / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014709:Vero Cells