The specific binding of beta-adrenergic ligands and morphofunctional properties of erythrocytes in bronchial asthma.
Peripheral blood erythrocytes from patients with bronchial asthma (BA) and preasthma (PA) were investigated using the following methods: 1) the radiochemical method for determining the specific binding of beta-adrenergic ligands; 2) direct erythrocytometry for erythrocyte diameter; and 3) the cytochemical method [paraldehyde fuchsin (PAF) reaction] for membrane glycoproteins. The specific binding of beta-adrenergic ligands in erythrocytes was decreased in BA and PA patients. The number of erythrocytes of small diameter (ESD) showed a tendency to increase in patients with atopic BA and extrapulmonary allergies, as well as in healthy subjects with a family history of atopy. The percent of PAF-negative erythrocytes was found to be considerably increased in atopic BA. It is concluded that general disorders of the membrane-receptor system are a fundamental cause of reactivity changes in allergy.
Zhikharev
S
S
Pertseva
M N
MN
Kuznetsova
L A
LA
Mineev
V N
VN
Yablonskaya
V N
VN
Bogdanova YuM
D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000097:blood*; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D020120:Iodocyanopindolol; D008297:Male; D008875:Middle Aged; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D006023:Glycoproteins; D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D010869:Pindolol