[Isolation of site-specific endonucleases and methylases from Bacillus cereus 83].
The site-specific endonuclease R Bce83I and methylase M Bce83I were isolated from Bacillus cereus 83 by three consecutive chromatographies on blue agarose, hydroxyapatite and heparin-Sepharose. R Bce83I recognizes [formula: see text] sequences on the DNA and cleaves the DNA as indicated by the arrows. The endonuclease is stimulated by S-adenosyl-L-methionine and may consequently be referred to type IV restriction enzymes.
Matvienko
N N
NN
Kramarov
V M
VM
Zheleznaia
L A
LA
Matvienko
N I
NI
D001409:Bacillus cereus / Q000201:enzymology*; D002853:Chromatography, Liquid; D004247:DNA / Q000378:metabolism; D015254:DNA Modification Methylases / Q000302:isolation & purification* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D015183:Restriction Mapping
D004247:DNA; D015254:DNA Modification Methylases; D004262:DNA Restriction Enzymes
Vydelenie saĭt-spetsificheskikh éndonukleazy i metilazy iz Bacillus cereus 83.