Glomerular expression and cell origin of transforming growth factor-beta 1 in anti-glomerular basement membrane disease.
The glomerular expression (mRNA levels) of transforming growth factor-beta 1 (TGF-beta 1) was assessed in two forms of rat anti-glomerular basement membrane (GBM) disease, a macrophage-independent and a macrophage-dependent variant. After a single intravenous injection of rabbit anti-rat GBM immune serum, significant proteinuria and histopathologic changes developed in both variants. Increased TGF-beta 1 mRNA levels were found in isolated glomeruli of the macrophage-dependent variant only in which glomerular infiltration by macrophages also occurred. Macrophages isolated from glomeruli of animals with this variant demonstrated TGF-beta 1 mRNA levels comparable to those found in glomeruli isolated at the same time point after injection of the anti-GBM serum. The observations indicate that in anti-GBM disease, enhanced glomerular TGF-beta 1 expression occurs in the macrophage-dependent variant and suggest that infiltrating macrophages account for this event.
Lianos
E A
EA
Medical College of Wisconsin, Froedtert Memorial Lutheran Hospital, Nephrology Division, Milwaukee.
Orphanos
V
V
Cattell
V
V
Cook
T
T
Anagnou
N
N
D000818:Animals; D001485:Basement Membrane / Q000276:immunology; D015433:Glomerulonephritis, Membranous / Q000378:metabolism* / Q000473:pathology; D007678:Kidney Glomerulus / Q000737:chemistry* / Q000276:immunology*; D008264:Macrophages / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta