title: The influence of the maternal thyroid hormone environment during pregnancy on the ontogenesis of brain and placental ornithine decarboxylase activity in the rat.
abstract: The influence of maternal hypothyroxinaemia on early brain and placental development was examined in a partially thyroidectomized (parathyroid-spared; TX) rat dam model. Ornithine decarboxylase (ODC) specific activity, along with more general indices of cell growth, were determined in prenatal whole brain (at 15, 19 and 22 days of gestation), postnatal brain regions (at 5, 10 and 14 days) and placenta. Maternal hypothyroxinaemia resulted in reductions in fetal body weight, brain weight, brain DNA content and brain total protein content at 15 days of gestation; the latter effect persisting until 19 days of gestation. Further changes in brain cell growth were observed near term, when an increase in the DNA concentration was accompanied by a decrease in the total protein:DNA ratio. Growth of the postnatal brain regions appeared normal, with the exception of an isolated increase in the protein content of the cerebellum at postnatal day 5. Determination of the specific activity of brain ODC revealed a complex pattern of change in the progeny of TX dams, superimposed upon the normal ontogenetic decline. In the fetal brain, activity was initially deficient at 15 days of gestation but was increased at 22 days of gestation relative to controls. The compromise extended into the postnatal period; ODC specific activity being transiently reduced in the brainstem, the subcortex and the cerebral cortex. Placental development was less consistently affected; wet weight, gross indices of cell growth (DNA content, DNA concentration, total protein:DNA ratio) and ODC specific activity were all normal in the TX dam. However, cytosolic and total protein concentrations were reduced at 15 and 19 days of gestation respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Molecular Endocrinology, University College London Medical School, U.K.
authors.forename: M R
authors.initials: MR
authors.lastname: Pickard
authors.forename: A K
authors.initials: AK
authors.lastname: Sinha
authors.forename: L
authors.initials: L
authors.lastname: Ogilvie
authors.forename: R P
authors.initials: RP
authors.lastname: Ekins
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology / Q000196:embryology* / Q000201:enzymology; D001923:Brain Chemistry; D004247:DNA / Q000032:analysis; D005260:Female; D007037:Hypothyroidism / Q000378:metabolism*; D009929:Organ Size / Q000502:physiology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013965:Thyroidectomy
chemical_list: D011506:Proteins; D004247:DNA; D009955:Ornithine Decarboxylase