The sequence of the Haemophilus influenzae mutB gene indicates it encodes a DNA helicase II-like protein.
A 6.2-kb Haemophilus influenzae genomic DNA fragment which partially complemented both the mutator and ultraviolet light sensitive (UVs) phenotypes of the H. influenzae mutB1 mutant was isolated. This fragment was also able to complement the UVs phenotype of Escherichia coli uvrD mutant hosts. The uvrD+ gene complemented the mutator phenotype of mutB1 hosts. The nucleotide (nt) sequence of the 6.2-kb fragment revealed an open reading frame (ORF) of 2184 bp. This ORF shows similarity at both the nt and amino acid (aa) levels with the uvrD gene of E. coli. Comparison of the sequences revealed eight regions of aa conservation in addition to seven previously identified helicase superfamily domains. The nt sequence 5' to the mutB ORF contains several potential regulatory motifs, including a LexA-binding site. Based upon these observations, we are confident that the mutB gene of H. influenzae encodes an ATP-dependent DNA helicase-like activity.
Walter
R B
RB
Department of Biology, Southwest Texas State University, San Marcos 78666.
Morton
K A
KA
Stuy
J H
JH
D000251:Adenosine Triphosphatases / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004265:DNA Helicases / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006193:Haemophilus influenzae / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D012045:Regulatory Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D000251:Adenosine Triphosphatases; C085509:MutB protein, Haemophilus influenzae; C506746:UvrD protein, E coli; D004265:DNA Helicases