Methotrexate-insensitive mutants of human dihydrofolate reductase (hDHFR) constructed by site-directed mutagenesis at phenylalanine 34.
Nakano
T
T
Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, TN 38101.
Appleman
J R
JR
Blakley
R L
RL
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004351:Drug Resistance; D004926:Escherichia coli; D005492:Folic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D005493:Folic Acid Antagonists; D006801:Humans; D007700:Kinetics; D008727:Methotrexate / Q000494:pharmacology*; D016297:Mutagenesis, Site-Directed; D010649:Phenylalanine; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis
D005493:Folic Acid Antagonists; D011994:Recombinant Proteins; C010920:dihydrofolate; D010649:Phenylalanine; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate