The effect of systemic hyperinsulinemia with concomitant amino acid infusion on skeletal muscle protein turnover in the human forearm.
In vitro, insulin has been shown to increase skeletal muscle (SM) protein synthesis and decrease SM protein breakdown. Whether these same effects are found in vivo in man is less clear. The study of the effect of hyperinsulinemia (INS) on SM protein turnover (SMPT) is complicated by hypoaminoacidemia, which can obviate the true effect of insulin on SMPT. To prevent this, we studied the effect of INS on SMPT in the human forearm with amino acid (AA) infusion to ensure adequate substrate for full evaluation of insulin's effect. Twelve healthy volunteers (aged 53 +/- 3 years) were studied. Steady-state AA kinetics were measured across the forearm after a systemic 2-hour primed continuous infusion of 3H-phenylalanine (3H-Phe) and 14C-leucine (14C-Leu) in the postabsorptive (PA) state and in response to systemic INS (71 +/- 5 microU/mL). AAs were infused during INS as 10% Travasol (Travenol Laboratories, Deerfield, IL) at .011 mL/kg/min to maintain PA branched-chain AA (BCAA) levels, known regulators of SMPT, and to mildly elevate total AA levels. The negative PA net balance of both Phe and total Leu carbons (LeuC) became positive with INS + AA infusion (Phe from -16 +/- 2 to 12 +/- 3 nmol/min/100 g [P < .01]; LeuC from -26 +/- 6 to 24 +/- 7 nmol/min/100 g [P < .01]).(ABSTRACT TRUNCATED AT 250 WORDS)
Newman
E
E
Surgical Metabolism Laboratory, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Heslin
M J
MJ
Wolf
R F
RF
Pisters
P W
PW
Brennan
M F
MF
D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D000597:Amino Acids, Branched-Chain / Q000032:analysis / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005542:Forearm; D005934:Glucagon / Q000097:blood; D006801:Humans; D006946:Hyperinsulinism / Q000097:blood* / Q000378:metabolism* / Q000503:physiopathology; D007328:Insulin / Q000097:blood / Q000494:pharmacology; D007930:Leucine / Q000032:analysis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism*; D009132:Muscles / Q000737:chemistry / Q000378:metabolism; D010649:Phenylalanine / Q000032:analysis / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D001786:Blood Glucose; D002250:Carbon Radioisotopes; D007328:Insulin; D009124:Muscle Proteins; D014316:Tritium; D010649:Phenylalanine; D005934:Glucagon; D007930:Leucine