Contraction of bovine isolated bronchial airways: effects of epithelium removal.
The influence of the epithelium on the contractile responses of bovine bronchial rings to acetylcholine, carbachol, 5-hydroxytryptamine and histamine was studied. Epithelium removal caused a significant leftward shift of the acetylcholine concentration-effect curve, pD2 values were 2.86 +/- 0.11 and 3.34 +/- 0.18 for intact and rubbed tissue, respectively (P < 0.05). However, there was no change in the acetylcholine maximal response. The sensitivity and responsiveness of the bovine bronchus to the other spasmogens were not significantly altered by removal of the epithelium. These results suggest that the epithelium of bovine bronchi modulates only acetylcholine-induced contraction.
Ortiz
J L
JL
Departamento de Farmacologia, Facultad de Medicina, Valencia, Spain.
Labat
C
C
Norel
X
X
Matera
M G
MG
Cortigo
J
J
Morcillo
E
E
Rossi
F
F
Brink
C
C
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000502:physiology; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D012701:Serotonin / Q000494:pharmacology
D012701:Serotonin; D006632:Histamine; D002217:Carbachol; D000109:Acetylcholine