title: Significance of pH on differentiation of human erythroid cell lines.
abstract: As physiological factors and compounds, BSA and/or higher pH of the culture medium could induce the erythroid differentiation of cells. Optimum pH values of the culture medium for higher spontaneous differentiation of KU-812 and K562 cells after 7 days cultivation were 7.5 and 7.6, respectively. The synergistic effects on the differentiation were observed by exposure to hemin under the higher pH condition. In the presence of BSA, 25% of KU-812 and 40% of K562 cells became benzidine positive. Synergistic effects of BSA and the higher pH of the medium were also observed.
authors.affiliation: Department of School-Nurse Training, Kyushu Women's Junior College, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: Y
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: H
authors.initials: H
authors.lastname: Okana
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumaki
mesh_terms: D002454:Cell Differentiation; D003470:Culture Media; D004912:Erythrocytes / Q000737:chemistry / Q000473:pathology*; D006427:Hemin / Q000494:pharmacology; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology
chemical_list: D003470:Culture Media; D006454:Hemoglobins; D012710:Serum Albumin, Bovine; D006427:Hemin