title: The time course of intracellular calcium movements in single human umbilical vein smooth muscle cells.
abstract: Intracellular Ca2+ ([Ca2+]i) was measured in single isolated human umbilical vein smooth muscle cells. Stimulation with histamine, in the absence of external Ca2+, mobilised Ca2+ from intracellular stores. When repeated brief applications of agonist were used, the time to onset, amplitude and rate of rise of the Ca2+ transients were found to change. Two components could often be discerned in the rising phase of the transients, an initial slow "pacemaker" and a second faster and larger component. Following the first histamine-activated transient the basal level of [Ca2+]i was invariably lower than that prior to stimulation. This lower value was maintained whilst the cell remained in Ca(2+)-free solution, but could be returned to a higher level if the cell was exposed to external Ca2+. When the mobilisation of the intracellular store was reduced to undetectable levels, re-exposure to Ca(2+)-containing medium reactivated responses. In the absence of external Ca2+, continuous application of histamine activated a series of transient increases in intracellular Ca2+, which decreased progressively in amplitude and rate of rise. The interval between transients also increased. These findings are discussed in terms of the activation of inositol trisphosphate-sensitive intracellular Ca2+ stores and their sensitivity to cytoplasmic Ca2+ and intrasarcoplasmic reticulum Ca2+.
authors.affiliation: Department of Physiological Sciences, Medical School, University, Newcastle upon Tyne, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Nicholls
authors.forename: J I
authors.initials: JI
authors.lastname: Gillespie
authors.forename: J R
authors.initials: JR
authors.lastname: Greenwell
mesh_terms: D002118:Calcium / Q000378:metabolism*; D016257:Fura-2; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism
chemical_list: D006632:Histamine; D002118:Calcium; D016257:Fura-2