Identification of a monoclonal antibody reactive with the bovine orthologue of CD3 (BoCD3).
A monoclonal antibody (mAb), MM1A, that identifies a molecule expressed on a large percentage of bovine lymphocytes (60-80%) was examined to determine its specificity. Two and three colour immunofluorescence analysis using flow cytometry revealed the molecule is highly expressed on all CD4+ and CD8+ lymphocytes and lymphocytes that express WC1 and the gamma/delta TCR. In contrast to mAbs reactive with BoCD5, MM1A did not react with B lymphocytes. Biochemical analysis revealed that the mAb immunoprecipitates a molecular complex comprising a set of peptides with M(r) approximately 12, 16, 22, 32, 36, and 44 kDa under reducing conditions and an additional 96 kDa peptide complex under non-reducing conditions. The data indicate that MM1A recognizes the bovine orthologue of CD3 (BoCD3).
Davis
W C
WC
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
MacHugh
N D
ND
Park
Y H
YH
Hamilton
M J
MJ
Wyatt
C R
CR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D003429:Cross Reactions; D005434:Flow Cytometry / Q000662:veterinary; D006041:Goats / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D012756:Sheep / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD