Atomic force microscopy of mammalian sperm chromatin.
We have used the atomic force microscope (AFM) to image the surfaces of intact bull, mouse and rat sperm chromatin and partially decondensed mouse sperm chromatin attached to coverglass. High resolution AFM imaging was performed in air and saline using uncoated, unfixed and unstained chromatin. Images of the surfaces of intact chromatin from all three species and of an AFM-dissected bull sperm nucleus have revealed that the DNA is organized into large nodular subunits, which vary in diameter between 50 and 100 nm. Other images of partially decondensed mouse sperm chromatin show that the nodules are arranged along thick fibers that loop out away from the nucleus upon decondensation. These fibers appear to stretch or unravel, generating narrow smooth fibers with thicknesses equivalent to a single DNA-protamine complex. High resolution AFM images of the nodular subunits suggest that they are discrete, ellipsoid-shaped DNA packaging units possibly only one level of packaging above the protamine-DNA complex.
Allen
M J
MJ
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94550.
Lee
C
C
Lee
J D
JD
Pogany
G C
GC
Balooch
M
M
Siekhaus
W J
WJ
Balhorn
R
R
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000648:ultrastructure; D008297:Male; D051379:Mice; D016252:Microscopy, Scanning Tunneling / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D013045:Species Specificity; D013094:Spermatozoa / Q000648:ultrastructure*
D002843:Chromatin; D004247:DNA