Aromatic DNA adducts in brain tumors by 32P-postlabeling analysis.
DNA from human brain tumor samples was analysed by the 32P-postlabeling technique for the presence of aromatic DNA adducts. Thirteen out of 16 samples showed low levels of adducts at 0.14-3.53 adducts per 10(9) nucleotides. Inter-individual variations in the patterns of these aromatic adducts were observed. On the other hand, none of 5 brain samples from epilepsy patients revealed any evidence of such adducts. The data demonstrated the presence of low level, large molecule aromatic DNA adducts in malignant brain tissues and these adducts may either result from environmental exposure to an undetermined genotoxic agent or from the aging process.
Lee
L S
LS
Neurological Institute, Veterans General Hospital-Taipei, Taiwan, Republic of China.
Chou
M D
MD
Chi
C W
CW
Liu
H C
HC
Shih
Y H
YH
Liu
T Y
TY
D000236:Adenoma / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002855:Chromatography, Thin Layer; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000378:metabolism*; D004827:Epilepsy / Q000378:metabolism; D005260:Female; D005910:Glioma / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008579:Meningioma / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009442:Neurilemmoma / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010911:Pituitary Neoplasms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011083:Polycyclic Compounds / Q000378:metabolism* / Q000633:toxicity
D002273:Carcinogens; D004273:DNA, Neoplasm; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds; D003577:Cytochrome P-450 Enzyme System