title: A murine CDC25/ras-GRF-related protein implicated in Ras regulation.
abstract: A partial cDNA encoding a novel putative p2, ras guanine nucleotide release-inducing factor (GRF), GRF2, was amplified from murine embryonic stem cells. The presumptive catalytic region of GRF2 is related to the yeast Ras GRF encoded by CDC25. GRF2 is 80% identical to murine CDC25Mm/ras-GRF, but is more similar to yeast CDC25 than to other ras GRFs related to the Drosophila son of sevenless gene product. A 9-kb GRF2 messenger RNA was highly expressed in brain, but GRF2-specific antibodies recognized apparent GRF2 proteins in various mouse tissues in addition to brain. Thus GRF2 represents a novel widely-expressed protein that is highly related to CDC25Mm/ras-GRF, at least in its catalytic domain. Both GRF2 and CDC25Mm/ras-GRF are expressed in murine embryonic stem cells, suggesting that different Ras activators may regulate ras-dependent proliferation and differentiation in early mouse development.
authors.affiliation: Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: L J
authors.initials: LJ
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Greer
authors.forename: P S
authors.initials: PS
authors.lastname: Tung
authors.forename: M F
authors.initials: MF
authors.lastname: Moran
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D017124:Conserved Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D020662:Guanine Nucleotide Exchange Factors; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000378:metabolism; D020687:cdc25 Phosphatases; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D012333:RNA, Messenger; D020663:ras Guanine Nucleotide Exchange Factors; D020666:ras-GRF1; D020687:cdc25 Phosphatases