title: Lupus vulgaris confirmed by polymerase-chain reaction.
abstract: We report a case of lupus vulgaris with typical clinical and histological findings. Mycobacterium tuberculosis was not only identified by a conventional culture technique, but also by a recently established system which has been designed to detect mycobacterial DNA in formalin-fixed, paraffin-embedded tissue by polymerase-chain reaction (PCR). Because results can be obtained within days, the PCR-based technique may markedly facilitate the diagnosis of skin tuberculosis.
authors.affiliation: Department of Dermatology, Ludwig-Maximilians University, Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Steidl
authors.forename: U
authors.initials: U
authors.lastname: Neubert
authors.forename: M
authors.initials: M
authors.lastname: Volkenandt
authors.forename: R
authors.initials: R
authors.lastname: Chatelain
authors.forename: K
authors.initials: K
authors.lastname: Degitz
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D008177:Lupus Vulgaris / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012867:Skin / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial