[Effect of MHS-G, an amino acids granule, on hepatic encephalopathy in portacaval anastomosis rats].
Using portacaval anastomosis (PCA) rats as a model with or without injection of ammonium acetate, we investigated the effects of MHS-G on the abnormalities of electroencephalogram (EEG) and brain amines metabolism in comparison with those of SF-1008C, a commercial nutritional preparation for hepatic failure. MHS-G (0.68 g/kg, p.o.) clearly improved both the abnormalities of EEG (such as reduction of amplitude, increasing delta wave distribution and decreasing beta wave distribution) and brain amines metabolism (such as increasing of Trp and DOPAC content) after injection of ammonium acetate. Moreover, MHS-G significantly increased branched chain amino acid concentrations and decreased aromatic amino acid concentrations in plasma and brain in comparison with water, and it significantly decreased the ammonia level in plasma in comparison with water and SF-1008C. These results suggest that MHS-G has a positive effect on abnormalities of EEG and amino acids metabolism in the plasma and brain of PCA rats.
Inui
K
K
Research Laboratories, Roussel Morishita Co., Ltd., Shiga, Japan.
Matsuda
A
A
Chaki
H
H
Makita
I
I
Kokuba
Y
Y
Kataoka
M
M
Sato
M
M
D000085:Acetates / Q000008:administration & dosage / Q000493:pharmacokinetics; D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001921:Brain / Q000378:metabolism; D004569:Electroencephalography / Q000187:drug effects; D006501:Hepatic Encephalopathy / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D011167:Portacaval Shunt, Surgical; D051381:Rats
D000085:Acetates; D000596:Amino Acids; C049075:SF 1008C; C018824:ammonium acetate