The mechanism of interaction of ceruloplasmin with superoxide radicals.
1. The mechanism of interaction of CP with O2- radicals in chemical and enzymatic systems of superoxide radical generation as well as in the pulse radiolysis technique was studied. 2. It is found that CP does not exert any kinetic influence on the decomposition of superoxide radical and, unlike SOD, cannot catalyze the reaction of disproportionation of these radicals in systems with chemical and enzymatic generation of O2-. 3. The data obtained confirm the suggestion that CP interacts with precursors of O2- radicals. 4. The irradiation of CP does not change its inhibiting activity in the reaction of the formation of superoxide radicals in systems with enzymatic O2- generation, but decreases its oxidase activity. 5. The results obtained demonstrated that the increase in the radiation dose resulted in the decrease of the inhibiting activity of SOD, whereas the activity of CP did not change.
Sergeev
A G
AG
A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Pavlov
A R
AR
Revina
A A
AA
Yaropolov
A I
AI
D002570:Ceruloplasmin / Q000378:metabolism* / Q000528:radiation effects; D005609:Free Radicals; D006801:Humans; D011675:Pulse Radiolysis; D013481:Superoxides / Q000378:metabolism*
D005609:Free Radicals; D013481:Superoxides; D002570:Ceruloplasmin