Increased levels of tumor necrosis factor and interleukin 1 in bronchoalveolar lavage fluids from pigs infected with Mycoplasma hyopneumoniae.
We examined the levels of tumor necrosis factor (TNF)-alpha and interleukin-1 (IL-1) in bronchoalveolar lavage fluids (BALF) from pigs experimentally infected with Mycoplasma hyopneumoniae using biological assays with WEHI-164 cells and D10.G4.1 cells, respectively. Increased TNF-alpha and IL-1 in BALF were found in infected pigs with gross and/or microscopic lesions. A time-course study suggested TNF-alpha and IL-1 to be persistently elevated in infected pigs. Their presence in BALF would thus appear to be associated with the development of pneumonic lesions in M. hyopneumoniae infected pigs.
Asai
T
T
Zen-noh Institute of Animal Health, Chiba, Japan.
Okada
M
M
Ono
M
M
Irisawa
T
T
Mori
Y
Y
Yokomizo
Y
Y
Sato
S
S
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology* / Q000382:microbiology; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary; D005260:Female; D007375:Interleukin-1 / Q000032:analysis*; D009177:Mycoplasma pneumoniae / Q000276:immunology; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha