Comparison of three buffers used in the formulation of buffered charcoal yeast extract medium.
Growth of Legionella spp. on buffered charcoal yeast extract medium supplemented with alpha-ketoglutarate and formulated with 3-(n-morpholino)propanesulfonic acid (MOPS), 3-(n-morpholino)-2-hydroxypropanesulfonic acid (MOPSO), or n-(2-acetamido)-2-aminoethanesulfonic acid (ACES) buffer was similar. With three exceptions, growth was no different in buffered yeast extract broth supplemented with alpha-ketoglutarate and formulated with MOPS or ACES buffer.
Edelstein
P H
PH
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-4283.
Edelstein
M A
MA
D000818:Animals; D001431:Bacteriological Techniques; D002021:Buffers; D002606:Charcoal; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007875:Legionella / Q000254:growth & development*; D016952:Legionella pneumophila / Q000254:growth & development; D016676:Macrophages, Alveolar / Q000382:microbiology; D009025:Morpholines; D013654:Taurine / Q000031:analogs & derivatives
D002021:Buffers; D003470:Culture Media; D009025:Morpholines; D002606:Charcoal; D013654:Taurine; C008550:morpholinopropane sulfonic acid; C024789:N-(2-acetamido)-2-aminoethanesulfonic acid