title: Caffeine- and muscarinic receptor agonist-sensitive Ca2+ stores in chick ciliary ganglion cells.
abstract: To investigate the presence and the role of intracellular Ca2+ stores in chick ciliary ganglion cells, the concentration of cytosolic free Ca2+ ([Ca]in) was measured in acutely isolated neurons, using fura-2 microfluorometry. Caffeine caused a substantial increase in [Ca]in following or during high K+ depolarization; this response was inhibited by treatment of the cells with thapsigargin or with caffeine plus ryanodine. The peak value and the rate of the depolarization-induced [Ca]in increase were not much altered by either of these treatments, which deplete caffeine-sensitive Ca2+ stores. The muscarinic receptor agonists muscarine, oxotremorine M, and methacholine, caused substantial increases in [Ca]in, in a manner that was partially dependent on Ca2+. These agonists also caused a rise in [Ca]in during K+ depolarization, which rise was inhibited by treatment with thapsigargin or with caffeine plus ryanodine. The response to oxotremorine M during depolarization was strongly inhibited by 10 nM 4-DAMP, but was not inhibited by 1 microM pirenzepine or by 1 microM AF-DX 116. These results indicate that chick ciliary ganglion cells possess Ca2+ stores that are activated by both caffeine and a second messenger generated by the activation of the M3 muscarinic receptor subtype.
authors.affiliation: Department of Physiology, Kagoshima University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sorimachi
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D011976:Receptors, Muscarinic / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000187:drug effects; D012433:Ryanodine / Q000494:pharmacology; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
chemical_list: D011976:Receptors, Muscarinic; D011978:Receptors, Nicotinic; D013729:Terpenes; D012433:Ryanodine; D002110:Caffeine; D019284:Thapsigargin; D002118:Calcium