Neutrophil-specific antigen NB1 inhibits neutrophil-endothelial cell interactions.
Neutrophil-specific antigen NB1 is located on a 58 to 64 kd glycosyl phosphatidylinositol-linked plasma membrane glycoprotein. NB1 antigen can be detected on neutrophils from 97% of healthy volunteers, and NB1 antigen is expressed on subpopulations of neutrophils. Neutrophil subpopulations with varying functions have been described, and we hypothesize that NB1 antigen may play an important role in neutrophil function. We compared the function of NB1-positive and NB1-negative neutrophils obtained from several persons. There were no differences in the adhesion of NB1-positive and NB1-negative neutrophils incubated in C5a, N-formyl-Met-Leu-Phe (FMLP), phorbol myristate acetate (PMA), or buffer to type IV collagen, fibronectin, laminin, or polystyrene. However, the adherence to human umbilical vein endothelial cells (HUVEC) monolayers of unstimulated NB1-positive neutrophils was less than to NB1-negative neutrophils (20.0% +/- 4.2% vs 31.7% +/- 5.8%; p < 0.01). When neutrophils were stimulated with C5a, PMA, or FMLP, no differences were found in the adhesion of NB1-positive and NB1-negative cells to the same surfaces. When NB1-positive neutrophils were incubated with rabbit polyclonal anti-NB1 Fab fragments, their adherence to HUVEC was increased (32.9% +/- 10.1% vs 18.3% +/- 5.0%; p < 0.05). Fab fragments prepared from normal rabbit serum had no effect on neutrophil adherence to HUVEC. The chemotaxis of NB1-positive neutrophils to FMLP through nitrocellulose was significantly greater than that of NB1-negative neutrophils (p = 0.03), but there was no difference in chemotaxis to FMLP through polycarbonate membranes.(ABSTRACT TRUNCATED AT 250 WORDS)
Stroncek
D F
DF
American Red Cross St. Paul Regional Blood Services, MN.
Herr
G P
GP
Plachta
L B
LB
D000906:Antibodies / Q000494:pharmacology; D002448:Cell Adhesion; D002634:Chemotaxis, Leukocyte; D015936:Complement C5a / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D058851:GPI-Linked Proteins; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology / Q000502:physiology*; D007700:Kinetics; D008163:Luminescent Measurements; D008562:Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D009895:Opsonin Proteins; D011956:Receptors, Cell Surface; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D000906:Antibodies; C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007140:Immunoglobulin Fab Fragments; D007519:Isoantigens; D008562:Membrane Glycoproteins; D009895:Opsonin Proteins; D011956:Receptors, Cell Surface; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015936:Complement C5a; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate