Studies on silica-bonded monoclonal antibody packing material for separation of recombinant interferon by high performance immunoaffinity chromatography.
A method for the preparation of a new packing material for high performance liquid chromatography by bonding an anti-interferon monoclonal antibody to the surface of silica gel is described. The high coupling efficiency and activity of the interferon-alpha A-monoclonal antibody (IFN-alpha A-McAb) column were obtained by activated diol-silica gel with an activating agent. After purification with this packing material, the specific activity of recombinant human interferon-alpha A rose by up to 1.03 x 10(7)IU/mg protein and the purification efficiency was increased by approximately 100 times.
Feng
W
W
Department of Chemistry, Northwest University, Xi'an, China.
Geng
X
X
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000302:isolation & purification*; D011994:Recombinant Proteins; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D012822:Silicon Dioxide