Uptake of L-lactate by cultured rat brain neurons.
The uptake of L-lactate was investigated in neuronal primary cultures derived from embryonic rat brain with a radioactive tracer method. After preincubation of the cells in glucose-free buffer for 30 min, uptake increased with time for at least 10 min. A saturable component of uptake was found with half-maximal uptake at 10 mM lactate. This saturable component was abolished in the presence of 10 mM alpha-cyano-4-hydroxcinnamic acid. In addition, a non-saturable component dominated the uptake at high concentrations of lactate. Uptake was accelerated with decreasing pH, and was inhibited considerably by pyruvate. It is concluded that neurons are endowed with a lactate transport system which resembles in its properties the monocarboxylate carrier of peripheral tissues.
Dringen
R
R
Physiologisch-chemisches Institut, Universität Tübingen, FRG.
Wiesinger
H
H
Hamprecht
B
B
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003373:Coumaric Acids / Q000494:pharmacology; D005260:Female; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011773:Pyruvates / Q000494:pharmacology; D019289:Pyruvic Acid; D051381:Rats
D003373:Coumaric Acids; D007773:Lactates; D011773:Pyruvates; C007175:alpha-cyano-4-hydroxycinnamate; D019289:Pyruvic Acid