Flow cytometric analysis of hematuria using fluorescent antihemoglobin antibody.
To identify the morphological changes of urinary erythrocytes in hematuria objectively, flow cytometrical analysis of fluorescence-labeled erythrocytes was performed. Fifty-one fresh urine samples from 33 hematuric patients (16 with glomerulonephritis and 17 with urological disease) were obtained. Urine erythrocytes were stained with FITC-labeled antihemoglobin antibody, and distinguished from other particles with similar size. Forward scattered light intensity (FW-SC) was used as an indicator of red cell size and right orthogonally scattered light intensity (RT-SC) divided by forward scattering (RT-SC/FW-SC) was used as a marker of cell surface irregularity. The size of erythrocytes expressed by FW-SC was significantly smaller in glomerulonephritic hematuria (101.6 +/- 41.8) than that in urological hematuria (123.5 +/- 44.7). RT-SC/FW-SC was smaller in urological hematuria (1.22 +/- 0.18) in comparison with glomerulonephritic hematuria (1.33 +/- 0.12). These results suggest that erythrocytes in glomerulonephritic hematuria had smaller size and more complex surface structure in comparison with urological hematuria. When cutoff was set at 110 of FW-SC for the criteria of glomerulonephritic erythrocytes, a correct diagnosis was made in 73.3% (22/30) of glomerulonephritic hematuria and in 76.2% (16/21) of nonglomerular hematuria. We clarified more complex morphological changes of glomerulonephritic urinary erythrocytes objectively.
Tanaka
M
M
Third Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Kitamoto
Y
Y
Sato
T
T
Ishii
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies; D048429:Cell Size; D004912:Erythrocytes / Q000276:immunology / Q000473:pathology; D005069:Evaluation Studies as Topic; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005921:Glomerulonephritis / Q000150:complications / Q000652:urine; D006417:Hematuria / Q000150:complications / Q000652:urine*; D006454:Hemoglobins / Q000276:immunology*; D006801:Humans; D008027:Light; D008297:Male; D008875:Middle Aged; D012542:Scattering, Radiation; D014570:Urologic Diseases / Q000150:complications / Q000652:urine
D000906:Antibodies; D006454:Hemoglobins; D016650:Fluorescein-5-isothiocyanate