Identification of radiolabeled metabolites of nicotine in rat bile. Synthesis of S-(-)-nicotine N-glucuronide and direct separation of nicotine-derived conjugates using high-performance liquid chromatography.
Four metabolites of nicotine, including two glucuronides, have been separated by high-performance liquid chromatography. This separation was applied to identification of biliary metabolites of radiolabeled nicotine by radiometric detection. S-(-)-Nicotine N-glucuronide was synthesized and used as a standard in method development.
Seaton
M J
MJ
Department of Pharmacology, Pennsylvania State University, College of Medicine, Hershey 17033.
Vesell
E S
ES
Luo
H
H
Hawes
E M
EM
D000818:Animals; D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005965:Glucuronates / Q000138:chemical synthesis*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D009538:Nicotine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000138:chemical synthesis / Q000378:metabolism; D011874:Radiometry; D051381:Rats; D017207:Rats, Sprague-Dawley
D005965:Glucuronates; C085795:nicotine N-glucuronide; D009538:Nicotine