Identification and chromosomal mapping of a receptor tyrosine kinase with a putative phospholipid binding sequence in its ectodomain.
We have cloned a novel receptor tyrosine kinase that has an unusual ectodomain. The extracellular sequence consists of 416 amino acids and has none of the structural motifs that have been found in other receptor tyrosine kinases. The 150 amino acids in the amino terminus of the receptor is homologous to a putative phospholipid-binding sequence that is found also in other cell adhesion molecules such as the neuronal A5 antigen and coagulation factors V and VIII. The kinase domain has a short cytoplasmic tail and contains a short insert between subdomains I and II. The structure of this receptor kinase suggests that it belongs to a new family of receptors involved in cell-cell interactions. The cell adhesion kinase (Cak) is expressed at low levels in most adult tissues and expression is highest in the brain and lung. Using fluorescence in situ hybridization and interspecific backcross mapping, the Cak gene was localized to human chromosome 6 and mouse chromosome 17.
Perez
J L
JL
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854.
Shen
X
X
Finkernagel
S
S
Sciorra
L
L
Jenkins
N A
NA
Gilbert
D J
DJ
Copeland
N G
NG
Wong
T W
TW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002450:Cell Communication; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000032:analysis; D000070819:Discoidin Domain Receptor 1; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis* / Q000235:genetics; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics / Q000502:physiology
D018076:DNA, Complementary; D010743:Phospholipids; D011956:Receptors, Cell Surface; C092030:DDR1 protein, human; D000070819:Discoidin Domain Receptor 1; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases