Effects of prostaglandin E1 on the production of IgM and IgG class anti-dsDNA antibodies in NZB/W F1 mice.
OBJECTIVE
To investigate the effects of prostaglandin E1 (PGE1) on IgM and IgG class anti-dsDNA antibody production by young and aged female New Zealand black/white (NZB/W) F1 mouse spleen cells in vitro.


METHODS
Whole cells or B cells from NZB/W F1 mouse spleen cells were cultured with lipopolysaccharide (LPS) in the absence or presence of graded concentrations of PGE1 for 1 to 5 days. After cultures, the supernatants were collected and assayed for released IgM and IgG class anti-dsDNA antibodies by enzyme-linked immunosorbent assay.


RESULTS
Young (3-month-old) mouse spleen cells produced similar levels of IgM class anti-dsDNA antibodies, while these cells produced considerably low levels of IgG class anti-dsDNA antibodies compared to aged (6-month-old) mouse spleen cells when stimulated with LPS. PGE1 suppressed the production of IgM class anti-dsDNA antibodies by about 50% at a concentration of 10(-6) M in both young and aged mouse spleen cell cultures. On the other hand, the production of IgG class anti-dsDNA antibodies was resistant to the inhibitory effects of PGE1.


CONCLUSIONS
Our data suggest that PGE1 is effective in inhibiting the antibody synthesis by B cells precommitted to IgM class anti-dsDNA antibody production, but the production of IgG class anti-dsDNA antibody by memory B cells present in young and aged mice is resistant to the inhibitory effects of PGE1.
Yoshikawa
T
T
Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
Suzuki
H
H
Sugiyama
E
E
Tunru
I S
IS
Ikemoto
M
M
Hori
T
T
Matsui
S
S
Yamashita
N
N
Kobayashi
M
M
D000375:Aging / Q000276:immunology; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation / Q000187:drug effects; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D004247:DNA / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008814:Mice, Inbred NZB / Q000276:immunology*; D008817:Mice, Mutant Strains; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D004247:DNA; D000527:Alprostadil