title: Glycation of human lens crystallins: effect of age and aspirin treatment.
abstract: Human lenses of three different ages were used to study the effect of age and aspirin treatment on glycation of alpha-, beta- and gamma-crystallins. Soluble lens proteins were subjected to in vitro glycation with 5 mM [14C]glucose in the presence and absence of 10 mM aspirin. With crystallins from a 27-year-old lens alpha-crystallin was the most readily glycated protein. Glycation of all crystallins decreased substantially (37-77%) in 46- and 67-year-old lenses indicating an age-dependent decline in glycation sites. On the basis of a sensitive chemical assay for protein-bound glycogroups in lenses of 2-82 years of age this decline is apparently due to a 60% increase in in vivo glycation. Aspirin did not show any selectivity with regard to its ability to inhibit glycation of various crystallins. Irrespective of the age glycation of all crystallins was inhibited to a varying extent.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
authors.forename: M
authors.initials: M
authors.lastname: Cherian
authors.forename: E C
authors.initials: EC
authors.lastname: Abraham
mesh_terms: D000107:Acetylation; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D001241:Aspirin / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003459:Crystallins / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged
chemical_list: D003459:Crystallins; D005947:Glucose; D001241:Aspirin