title: Incurvation of early embryonic neural retina by acetylcholine through muscarinic receptors.
abstract: Acetylcholine could induce the incurvation of neural retina to form a cup-structure by muscarinic receptors in vitro. Neural retinae isolated from chick embryos were cup-shaped, but, it soon became flat in a normal bath solution. Then addition of acetylcholine caused an incurvation of the retina remaking the initial cup-structure. Ca2+ was involved in this movement since a calcium ionophore A23187 could also induce the folding of neural retina. Acetylcholine caused a rise in cytoplasmic Ca2+ concentration even in a Ca(2+)-free bath solution as measured with Fura-2. Atropine completely blocked both of the Ca2+ rise and the incurvation of neural retina by acetylcholine. It was suggested that the Ca2+ mobilization from intracellular reservoirs triggered the contractile movement to fold the embryonic neural retina.
authors.affiliation: Department of Physiology, Osaka University Medical School, Suita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamashita
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002642:Chick Embryo; D016257:Fura-2; D008856:Microscopy, Fluorescence; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D013997:Time Factors
chemical_list: D011976:Receptors, Muscarinic; D001285:Atropine; D000109:Acetylcholine; D002118:Calcium; D016257:Fura-2