title: Relationship between intracellular calcium and oxygen consumption: effects of perfusion pressure, extracellular calcium, dobutamine, and nifedipine.
abstract: All of the mechanisms that connect the cardiac mechanical work load with energy production have not been clearly defined. The purpose of this study was to evaluate the relationship between intracellular calcium and oxygen consumption in intact hearts, to further understand this relationship. Intracellular calcium was measured in isolated nonworking perfused rat hearts loaded with Indo-1 by means of a surface fluorometry technique. Glucose was used as a substrate. Myocardial contraction and oxygen consumption were modulated by perfusion pressure (80, 110, and 140 cm of water), extracellular calcium (1, 2, 3, and 4 mmol/L), dobutamine (10(-6) mol/L), and nifedipine (10(-6) mol/L). With all of these interventions there was a close correlation between intracellular calcium (systolic, diastolic, and amplitude) and oxygen consumption or left ventricular developed pressure. Observations in this study support the hypothesis that intracellular calcium plays a regulatory role in the link between cardiac mechanics and energy production.
authors.affiliation: Department of Medicine, University of California, San Francisco 94143-0124.
authors.forename: S
authors.initials: S
authors.lastname: Kojima
authors.forename: S T
authors.initials: ST
authors.lastname: Wu
authors.forename: W W
authors.initials: WW
authors.lastname: Parmley
authors.forename: J
authors.initials: J
authors.lastname: Wikman-Coffelt
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology*; D003326:Coronary Circulation / Q000502:physiology*; D003971:Diastole; D004280:Dobutamine / Q000494:pharmacology*; D005456:Fluorescent Dyes; D006321:Heart / Q000187:drug effects; D007211:Indoles; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism*; D009543:Nifedipine / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013599:Systole; D017725:Ventricular Pressure / Q000187:drug effects / Q000502:physiology
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D004280:Dobutamine; D009543:Nifedipine; C048960:indo-1; D002118:Calcium