title: Choline chloride activates time-dependent and time-independent K+ currents in dog atrial myocytes.
abstract: Using the whole cell configuration of the patch-clamp technique, we studied the effect of isotonic replacement of bath sodium chloride (NaCl) by choline chloride (ChCl) in dog atrial myocytes. Our results show that ChCl triggered 1) activation of a time-independent background current, characterized by a shift of the holding current in the outward direction at potentials positive to the K+ equilibrium potential (EK), and 2) activation of a time- and voltage-dependent outward current, following depolarizing voltage steps positive to EK. Because the choline-induced current obtained by depolarizing steps exhibited properties similar to the delayed rectifier K+ current (IK), we named it IKCh. The amplitude of IKCh was determined by extracellular ChCl concentration, and this current was generally undetectable in the absence of ChCl. IKCh was not activated by acetylcholine (0.001-1.0 mM) or carbachol (10 microM) and could not be recorded in the absence of ChCl or when external NaCl was replaced by sucrose or tetramethylammonium chloride. IKCh was inhibited by atropine (0.01-1.0 microM) but not by the M1 antagonist pirenzepine (up to 10 microM). This current was carried mainly by K+ and was inhibited by CsCl (120 mM, in the pipette) or barium (1 mM, in the bath). We conclude that in dog atrial myocytes, ChCl activates a background conductance comparable to ACh-dependent K+ current, together with a time-dependent K+ current showing properties similar to IK.
authors.affiliation: Montreal Heart Institute, Department of Medicine, University of Montreal, Quebec, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Fermini
authors.forename: S
authors.initials: S
authors.lastname: Nattel
mesh_terms: D000200:Action Potentials; D000818:Animals; D016275:Atrial Function / Q000187:drug effects*; D002794:Choline / Q000494:pharmacology*; D004285:Dogs; D004594:Electrophysiology; D005260:Female; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000166:cytology; D011188:Potassium / Q000502:physiology*; D013997:Time Factors
chemical_list: D002794:Choline; D011188:Potassium