A novel cell culture technique for electron microscopy.
A simplified technique for the monolayer growth of cultured cells and their in situ embedment on the inner surface of the pyramidal portion of the Beem capsule for electron microscopy has been developed. The results demonstrated that the cell monolayers grew well on the surface of the Beem capsule and could be embedded in situ. Electron micrographs showed cells in their natural state of contact with one another. The plasma membrane and intracellular organelles were well preserved. This method minimizes many difficult steps and eliminates the disruption of cells by scraping, pelleting, or enzymatic reaction to remove them.
Wang
F
F
Mastology Research Institute, Baton Rouge, Louisiana 70816.
Ledford
L B
LB
Head
J F
JF
Elliott
R L
RL
D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*; D016610:Tissue Embedding; D014407:Tumor Cells, Cultured