PDGF-induces the glutathione-dependent enzyme PGH2/PGE2 isomerase in NIH3T3 and pEJ transformed fibroblasts.
Exposure of NIH3T3 and pEJ serum-starved cells to platelet derived growth factor results in a 16 fold increase in the glutathione-dependent enzyme prostaglandin H2/prostaglandin E2 isomerase activity (EC 5.3.99.3). The response is rapid as a detectable increase in NIH3T3 cells occurs after only 7 minutes of exposure to the growth factor. Only a mild increase in another microsomal glutathione-dependent enzyme, microsomal glutathione transferase (EC 2.5.1.18), was detected after a 2 hour exposure to the growth factor.
Kelner
M J
MJ
University of California, San Diego, Department of Pathology 92103-8320.
Uglik
S F
SF
D016475:3T3 Cells; D000818:Animals; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D015232:Dinoprostone / Q000032:analysis / Q000378:metabolism; D004790:Enzyme Induction; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D011905:Genes, ras; D005978:Glutathione / Q000494:pharmacology*; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000096:biosynthesis* / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008861:Microsomes / Q000201:enzymology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D000071876:Prostaglandin-E Synthases; D011863:Radioimmunoassay
D010982:Platelet-Derived Growth Factor; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D005978:Glutathione; D015232:Dinoprostone