An algorithm accounting for plating efficiency in estimating spontaneous mutation rates.
An algorithm is described for calculating the probability distribution of the number of mutant colonies arising in a Luria-Delbrück fluctuation experiment subject to a plating efficiency of less than 100%. A table is constructed to facilitate the estimation of spontaneous mutation rates taking into account the plating efficiency.
Jones
M E
ME
School of Medicine, Flinders University, Adelaide, Australia.
D000465:Algorithms; D002478:Cells, Cultured; D002999:Clone Cells; D008957:Models, Genetic; D009154:Mutation / Q000502:physiology*; D011336:Probability; D012641:Selection, Genetic