Isolation and structure of an acetolactate synthase gene from Schizosaccharomyces pombe and complementation of the ilv2 mutation in Saccharomyces cerevisiae.
A gene encoding a functional acetolactate synthase (ALS) subunit has been isolated from the fission yeast Schizosaccharomyces pombe, and has been structurally and genetically characterized. The approximate 5-kbp cloned DNA segment was found to contain a 2007-bp open reading frame capable of encoding a 669 aminoacid polypeptide which exhibited 57.1% similarity to the corresponding ALS subunit from Saccharomyces cerevisiae. The putative ilv1 isolated from S. pombe was shown to encode a functional subunit of acetolactate synthase by complementation of an S. cerevisiae strain deleted for the ILV2 locus.
Bekkaoui
F
F
Plant Biotechnology Institute, National Research Council of Canada, Saskatoon.
Nadin-Davis
S A
SA
Crosby
W L
WL
D000095:Acetolactate Synthase / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000302:isolation & purification / Q000378:metabolism; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics*
D017931:DNA Primers; D004271:DNA, Fungal; D046911:Macromolecular Substances; D000095:Acetolactate Synthase