title: Up-regulation of lipocortin-1 and its mRNA in reactive astrocytes in kainate-lesioned rat cerebellum.
abstract: We have used a combined molecular and immunocytochemical approach to examine the expression of lipocortin-1 (LC-1) in kainate-lesioned rat cerebellum. Using immunocytochemistry, Western and Northern blotting, we have shown upregulation of LC-1 mRNA and expression of LC-1 localised specifically to reactive astrocytes. These studies suggest that reactive astrocytes are a major synthetic compartment for the expression of LC-1. The well-reported immuno-suppressive effects of lipocortin(s), suggests that reactive astrocytes could serve to negatively modulate inflammatory reactions in the central nervous system.
authors.affiliation: Department of Medicine, Charing Cross & Westminster Medical School, London, UK.
authors.forename: L
authors.initials: L
authors.lastname: Mullens
authors.forename: D R
authors.initials: DR
authors.lastname: Marriott
authors.forename: K A
authors.initials: KA
authors.lastname: Young
authors.forename: L
authors.initials: L
authors.lastname: Tannahill
authors.forename: S L
authors.initials: SL
authors.lastname: Lightman
authors.forename: G P
authors.initials: GP
authors.lastname: Wilkin
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000032:analysis* / Q000235:genetics; D001253:Astrocytes / Q000737:chemistry*; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D002531:Cerebellum / Q000737:chemistry*; D007150:Immunohistochemistry; D007608:Kainic Acid; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
chemical_list: D017305:Annexin A1; D012333:RNA, Messenger; D007608:Kainic Acid