title: The invariant chain is required for intracellular transport and function of major histocompatibility complex class II molecules.
abstract: The major histocompatibility complex (MHC) class II-associated invariant chain (Ii) is thought to act as a chaperone that assists class II during folding, assembly, and transport. To define more precisely the role of Ii chain in regulating class II function, we have investigated in detail the biosynthesis, transport, and intracellular distribution of class II molecules in splenocytes from mice bearing a deletion of the Ii gene. As observed previously, the absence of Ii chain caused significant reduction in both class II-restricted antigen presentation and expression of class II molecules at the cell surface because of the intracellular accumulation of alpha and beta chains. Whereas much of the newly synthesized MHC molecules enter a high molecular weight aggregate characteristic of misfolded proteins, most of the alpha and beta chains form dimers and acquire epitopes characteristic of properly folded complexes. Although the complexes do not bind endogenously processed peptides, class II molecules that reach the surface are competent to bind peptides added to the medium, further demonstrating that at least some of the complexes fold properly. Similar to misfolded proteins, however, the alpha and beta chains are poorly terminally glycosylated, suggesting that they fail to reach the Golgi complex. As demonstrated by double label confocal and electron microscope immunocytochemistry, class II molecules were found in a subcompartment of the endoplasmic reticulum and in a population of small nonlysosomal vesicles possibly corresponding to the intermediate compartment or cis-Golgi network. Thus, although alpha and beta chains can fold and form dimers on their own, the absence of Ii chain causes them to be recognized as "misfolded" and retained in the same compartments as bona fide misfolded proteins.
authors.affiliation: Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: E A
authors.initials: EA
authors.lastname: Elliott
authors.forename: J R
authors.initials: JR
authors.lastname: Drake
authors.forename: S
authors.initials: S
authors.lastname: Amigorena
authors.forename: J
authors.initials: J
authors.lastname: Elsemore
authors.forename: P
authors.initials: P
authors.lastname: Webster
authors.forename: I
authors.initials: I
authors.lastname: Mellman
authors.forename: R A
authors.initials: RA
authors.lastname: Flavell
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte; D001692:Biological Transport; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005434:Flow Cytometry; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D011233:Precipitin Tests; D017510:Protein Folding; D015183:Restriction Mapping; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013234:Stem Cells
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; C045084:invariant chain