title: Protective immunity following immunisation of pigs with aerosol of Actinobacillus pleuropneumoniae serotype 2.
abstract: To introduce antigen to the respiratory mucosa, killed Actinobacillus pleuropneumoniae with quil A as adjuvant was administered to pigs as an aerosol. Immunisation by this aerosol induced a marked IgA response in the bronchoalveolar and nasal fluids, and in the serum. Following challenge with live bacteria two weeks after the last exposure to the aerosol, the immunised pigs were protected from the severe pleuropneumonia which developed in non-immunised pigs. The immunised pigs had lower antibody titres in the mucosal fluids and serum after exposure to the challenge. The immune response after experimental infection of non-immunised animals was a weak IgA antibody response in the bronchoalveolar and nasal fluids, whereas the systemic immune response after challenge included both IgA and IgG antibodies.
authors.affiliation: Department of Clinical Studies, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
authors.forename: M K
authors.initials: MK
authors.lastname: Loftager
authors.forename: L
authors.initials: L
authors.lastname: Eriksen
authors.forename: B
authors.initials: B
authors.lastname: Aasted
authors.forename: R
authors.initials: R
authors.lastname: Nielsen
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000276:immunology*; D000336:Aerosols; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D007114:Immunization / Q000662:veterinary*; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D017817:Nasal Lavage Fluid / Q000276:immunology; D011001:Pleuropneumonia / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000517:prevention & control
chemical_list: D000336:Aerosols; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G