Histone-induced condensation of rat testis chromatin: testis-specific H1t versus somatic H1 variants.
Due the likely role of H1 histone variants in inducing the formation of folded DNA filaments with different stabilities, the condensing capacity of the testis-specific H1t versus the somatic variants was tested. Circular dichroism analyses of rat testis H1-depleted oligonucleosomes (5-2kbp) revealed that H1t, which appears in germ cells during the meiotic prophase of mammalian spermatogenesis, exerts the lowest condensing effect as compared to the other variants. The distribution of H1 subtypes among different chromatin fractions was also investigated and gave evidence that H1t is more abundant in chromatin regions which are more sensitive to DNAase I digestion.
De Lucia
F
F
Dipartimento di Chimica Organica e Biologica, Universit Federico II, Napoli, Italy.
Faraone-Mennella
M R
MR
D'Erme
M
M
Quesada
P
P
Caiafa
P
P
Farina
B
B
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D014644:Genetic Variation; D006657:Histones / Q000494:pharmacology*; D008297:Male; D051381:Rats; D013737:Testis / Q000648:ultrastructure*
D002843:Chromatin; D006657:Histones; D004247:DNA