Spectra of the formaldehyde-induced ultraweak luminescence from yeast cells.
An increase in the intensity and distinct spectral changes of ultraweak luminescence from the yeast Saccharomyces cerevisiae were measured when the metabolism of cells was drastically altered. A small emission peak and a red emission band 680-850 nm appeared when air-dried cells were imbibed in water. Lethal concentrations of HCHO (0.01%-10%) elicited a 2500 fold increase of the emission intensity and distinct spectral alterations. A transient 500-580 nm emission appeared in the initial phase of interaction. Then a gradually increasing long-lasting red emission band centered around 620 nm predominated in the total spectral range covering 470-850 nm. These emissions were not correlated with minor changes in fluorescence emission and excitation spectra originating from tryptophan, flavins, and unidentified emitters.
Godlewski
M
M
Institute of Physics and Informatics, Pedagogical University, Krakow, Poland.
Rajfur
Z
Z
Sławiński
J
J
Kobayashi
M
M
Usa
M
M
Inaba
H
H
D005557:Formaldehyde / Q000494:pharmacology*; D007700:Kinetics; D008163:Luminescent Measurements; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D005557:Formaldehyde