T-cell receptor V beta 8 has no significant suppressive effect on collagen-induced arthritis.
To elucidate the in-vivo role of T lymphocytes in collagen-induced arthritis (CIA), treatment with anti-CD4 or anti-T-cell receptor (TCR) V beta 8 monoclonal antibody (mAb) was performed in CIA mice. As the result of injection with each mAb, CD4-positive cells were incompletely deleted in the peripheral blood and lymph nodes, whereas TCR-V beta 8-positive cells were completely deleted. In the anti-CD4-mAb-treated mice, the development of arthritis was markedly suppressed in association with a lower antibody response to type II collagen. However, the treatment with anti-TCR-V beta 8 mAb did not alter either the arthritis symptoms or the antibody response. These results suggest that the development of arthritis is regulated by T cells, most probably CD4-positive cells, and not by TCR-V beta 8-positive cells.
Matsuno
H
H
Department of Orthopaedic Surgery, Toyama Medical and Pharmaceutical University, Japan.
Kadowaki
K M
KM
Suzuki
H
H
Ochiai
H
H
Tsuji
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D001168:Arthritis / Q000139:chemically induced / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology*; D003094:Collagen / Q000276:immunology*; D005434:Flow Cytometry; D016131:Lymphocyte Subsets; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D003094:Collagen