title: Analysis of the influence of chromosomal condensation on the activity of restriction endonucleases.
abstract: The different responses of metaphase chromosomes to restriction endonuclease activity depend on the stage of chromosomal condensation. Results obtained established that this factor plays a remarkable role in obtaining high resolution banding patterns which facilitated visualization of a large number of sub-bands. It is evident that various restriction endonucleases, which are able to induce a coincident G-like banding pattern on Don cell line chromosomes, act in very different ways.
authors.affiliation: Departamento de Biología Celular y Molecular, Area de Genética, Universidad de la Coruña, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: González-Tizón
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Lage
authors.forename: J
authors.initials: J
authors.lastname: Méndez
mesh_terms: D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002875:Chromosomes / Q000378:metabolism* / Q000648:ultrastructure; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D015247:Deoxyribonuclease HindIII / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008677:Metaphase
chemical_list: D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases