Zinc, a structural component of adenylate kinases from gram-positive bacteria.
The recent finding that Bacillus stearothermophilus adenylate kinase contains a zinc atom coordinated to four cysteines prompted us to investigate the metal-binding properties of the enzyme from various bacteria. We conclude that zinc was present only in adenylate kinase from gram-positive species and that this property is correlated with the presence of three or four Cys residues in the sequence Cys-X2-Cys-X16-Cys-X2-Cys/Asp, in which X stands for different amino acid residues.
Gilles
A M
AM
Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France.
Glaser
P
P
Perrier
V
V
Meier
A
A
Longin
R
R
Sebald
M
M
Maignan
L
L
Pistotnik
E
E
Bârzu
O
O
D000263:Adenylate Kinase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000032:analysis* / Q000378:metabolism
D000263:Adenylate Kinase; D015032:Zinc; D003545:Cysteine