Enzyme immunoassays for ovarian steroid metabolites in the urine of Macaca fascicularis.
In vivo studies using carbon 14 labeled estradiol (E2) and progesterone (Po) were performed to characterize the time course and metabolic fate of circulating E2 and Po. Co-chromatography of human, orangutan, and macaque luteal phase urine samples demonstrated the presence of a steroid conjugate peak in all three species that was identified as being androsterone and etiocholanolone glucuronides. An enzyme immunoassay for urinary metabolites of Po was developed subsequently for Macaca spp. using a monoclonal antibody that cross-reacted with both C-19 and C-21 metabolites.
Shideler
S E
SE
Institute of Toxicology and Environmental Health, University of California, Davis 95616.
Shackleton
C H
CH
Moràn
F M
FM
Stauffer
P
P
Lohstroh
P N
PN
Lasley
B L
BL
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004958:Estradiol / Q000378:metabolism* / Q000652:urine; D005243:Feces / Q000737:chemistry; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008252:Macaca fascicularis / Q000378:metabolism* / Q000652:urine; D008253:Macaca mulatta / Q000378:metabolism* / Q000652:urine; D008597:Menstrual Cycle / Q000378:metabolism; D011148:Pongo pygmaeus; D011374:Progesterone / Q000378:metabolism* / Q000652:urine
D011374:Progesterone; D004958:Estradiol