Capillary-zone electrophoretic analyses of the proteins and amino acid components in cerebrospinal fluid of central nervous system diseases.
Analysis systems for protein and amino acid components in cerebrospinal fluid (CSF) from patients with a variety of central nervous system (CNS) diseases were established employing capillary-zone electrophoresis (CZE). Under the conditions employed, the major proteins in CSF and the concurrent sera were separated into several respective fractions, and in the obtained electropherograms of serum proteins, the % values of areas of each major protein fraction (from gamma-globulin to albumin) were in close accord with those in the densitograms of stained protein bands after electrophoresis on cellulose-acetate strips which had been done as a routine laboratory test in the hospital. Some unusual migration patterns of major proteins were observed in various CNS diseases, and an increase of glutamine in hepatic encephalitis was also detected on the electropherograms. A peak responsible for a minor protein component with the molecular weight of 10000--30000, which was tentatively identified as beta-trace protein, was found to be contained in all the CSF samples examined, and its concentration levels were higher in some patients with cerebral infarction and multiple sclerosis. These results suggest that CZE can become a powerful aid in analyses of the protein and amino acid components in CSF for biochemical diagnosis of CNS diseases.
Hiraoka
A
A
Kyorin University School of Health Sciences, Tokyo, Japan.
Miura
I
I
Hattori
M
M
Tominaga
I
I
Machida
S
S
D000596:Amino Acids / Q000134:cerebrospinal fluid*; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D004586:Electrophoresis; D006801:Humans; D008970:Molecular Weight
D000596:Amino Acids; D002556:Cerebrospinal Fluid Proteins