Elicitation of a predominantly lambda light chain-bearing antibody response in BALB/c mice to a novel bifunctional aminocarboxylate chelating agent.
A panel of murine monoclonal antibodies has been raised against the aminocarboxylate bifunctional chelating agent PA-DOTA complexed with Samarium. Ninety percent of the antibodies (43 of 48) used a lambda light chain. The binding specificity of 11 of the antibodies was examined by competition assays using several structurally related aminocarboxylate chelating agents containing different metals. Most of the antibodies are directed against the macrocycle ring of PA-DOTA and require the presence of a metal within the structure for binding.
Anderson
W H
WH
Bioproducts Laboratory, Dow Chemical Company, Midland, MI 48674.
Reed
J A
JA
Pollock
D K
DK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D002614:Chelating Agents; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007142:Immunoglobulin gamma-Chains / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D007142:Immunoglobulin gamma-Chains; D007146:Immunoglobulin lambda-Chains