Chiral high-performance liquid chromatographic determination of oxprenolol in plasma.
A sensitive, stereospecific high-performance liquid chromatographic assay for oxprenolol enantiomers in rat plasma was developed, using a chiral derivatization agent. Racemic oxprenolol and the internal standard (racemic propranolol) are extracted with dichloromethane after alkalinization of the plasma. Quantitation of R(+)- and S(-)-oxprenolol is based on derivatization with the chiral agent S(-)-1-(1-naphthyl)-ethyl isocyanate, followed by chromatographic separation on a C18 reversed-phase column, with fluorometric detection (excitation at 226 nm, emission at 333 nm). The assay is reproducible as judged by a coefficient of variation of less than 17.5% for both enantiomers at all concentrations used. Preliminary experiments in the rat demonstrate that the method is sufficiently sensitive for pharmacokinetic studies in that species.
Laethem
M E
ME
Heymans Institute of Pharmacology, University of Gent Medical School, Belgium.
Rosseel
M T
MT
Wijnant
P
P
Belpaire
F M
FM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D007202:Indicators and Reagents; D017953:Isocyanates; D008297:Male; D009281:Naphthalenes; D010096:Oxprenolol / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D011433:Propranolol / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D007202:Indicators and Reagents; D017953:Isocyanates; D009281:Naphthalenes; C054308:1-(1-naphthyl)ethyl isocyanate; D010096:Oxprenolol; D011433:Propranolol