Isolation of suppressors of temperature-sensitive folding mutations.
Mutations in the tailspike gene (gene 9) of Salmonella typhimurium phage P22 have been used to identify amino acid interactions during the folding of a polypeptide chain. Since temperature-sensitive folding (tsf) mutations cause folding defects in the P22 tailspike polypeptide chain, it is likely that mutants derived from these and correcting the original tsf defects (second-site intragenic suppressors) identify interactions during the folding pathway. We report the isolation and identification of second-site revertants to tsf mutants.
Villafane
R
R
Department of Microbiology, University of Tennessee, Knoxville 37996.
Fleming
A
A
Haase-Pettingell
C
C
D017100:Bacteriophage P22 / Q000235:genetics*; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D016153:Genes, Suppressor / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009154:Mutation; D017510:Protein Folding; D012486:Salmonella typhimurium; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000235:genetics*; D017376:Viral Tail Proteins
D014764:Viral Proteins; D015678:Viral Structural Proteins; D017376:Viral Tail Proteins; D006026:Glycoside Hydrolases; C083268:tailspike protein, bacteriophage