Functional analysis of mononuclear cells infiltrating into tumors: establishment of T cell hybridomas exhibiting distinct interacting abilities with endothelial cells and extracellular matrix components.
We have established eleven T cell hybridoma cell lines to investigate mechanisms controlling interaction of T lymphocytes with endothelial cells as well as extracellular matrix (ECM) proteins at the clonal level. T cell hybridomas were characterized and subdivided into four groups on the basis of their interaction behavior with high endothelial venules (HEV). Group 1 (G1) exhibited strong adhesiveness. The binding was temperature- and divalent cation-dependent. Group 2 exhibited both adhesiveness and transendothelial migration (TEM, i.e., transmigration beneath the cytoplasm of endothelial cells). Group 3 exhibited strong TEM. G2 and G3 hybridomas exhibited temperature-independent and divalent cation-independent binding to HEV. Group 4 exhibited nonspecific adhesiveness to the surface of a slide glass. BW 5147, a parent of T cell hybridomas, was classified as G4. TEM was dependent on both the nature of T cell hybridomas and endothelial cells. TEM was completely temperature-dependent. TEM of G3 hybridomas was not divalent cation-dependent. Each group of T cell hybridomas interacted with various ECM components.
Uno
E
E
Department of Internal Medicine (Section 3), Sapporo Medical University School of Medicine.
Kikuchi
K
K
Saiki
I
I
Uede
T
T
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000166:cytology / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology*; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D013601:T-Lymphocytes / Q000502:physiology*
D016326:Extracellular Matrix Proteins