Plasmin converts pro-form of group I phospholipase A2 into receptor binding, active forms.
Treatment of zymogen of pancreatic-type group I phospholipase A2 (PLA2-I) by plasmin, a fibrinolytic enzyme, increases PLA2 activity as well as receptor binding activity in a dose- and time-dependent manner. Separation of plasmin-treated pro-PLA2-I by HPLC and amino acid sequence analysis of the products revealed that, in addition to an authentic mature PLA2-I produced by trypsin, plasmin produced active products which had been modified in the C-terminal region. Thus, PLA2-I may be involved in physiologic processes which accompany the formation of plasmin.
Nakano
T
T
Shionogi Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan.
Fujita
H
H
Kikuchi
N
N
Arita
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004792:Enzyme Precursors / Q000378:metabolism*; D005341:Fibrinolysin / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments / Q000302:isolation & purification; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011498:Protein Precursors / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D054507:Receptors, Phospholipase A2; D013552:Swine; D014357:Trypsin / Q000378:metabolism
D004792:Enzyme Precursors; D007527:Isoenzymes; D010446:Peptide Fragments; D011498:Protein Precursors; D011956:Receptors, Cell Surface; D054507:Receptors, Phospholipase A2; D010741:Phospholipases A; D054467:Phospholipases A2; C085333:prophospholipase A2; D014357:Trypsin; D005341:Fibrinolysin