title: Cloning and sequencing of genomic DNA extracted from archival human temporal bone sections.
abstract: Cloning techniques allow the engineering and production of highly purified DNA. Further advances in molecular biology have provided the means to identify DNA sequences in a rapid fashion. Sequencing methods can identify mutations, deletions, polymorphisms, or confirm a known genetic sequence. The use of these techniques in clinical medicine has made it possible to accurately diagnose infectious diseases and determine the molecular etiology of many genetic disorders and malignancies. In this study, DNA extracted from archival, celloidin-embedded temporal bone sections has been cloned and sequenced using these techniques. We amplified, cloned, and sequenced varicella-zoster viral DNA extracted from archival temporal bone sections from patients who had herpes zoster oticus. The application of cloning and sequencing techniques to DNA extracted from archival temporal bones provides the methodology to study temporal bone pathology at the molecular level.
authors.affiliation: Laboratory of Molecular Biology, UCLA School of Medicine 90024-1794.
authors.forename: M M
authors.initials: MM
authors.lastname: Kerner
authors.forename: P A
authors.initials: PA
authors.lastname: Wackym
authors.forename: P
authors.initials: P
authors.lastname: Popper
authors.forename: D E
authors.initials: DE
authors.lastname: Tabor
authors.forename: W W
authors.initials: WW
authors.lastname: Grody
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D016697:Herpes Zoster Oticus / Q000175:diagnosis*; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013701:Temporal Bone / Q000473:pathology*
chemical_list: D004279:DNA, Viral