Analysis of protective and nonprotective monoclonal antibodies specific for Bordetella pertussis lipooligosaccharide.
In this study, it has been determined that immunoglobulin G1 (IgG1) and IgG3 monoclonal antibodies directed to the lipooligosaccharide A of Bordetella pertussis were able to protect mice from fatal aerosol infection. No correlation was found between the bactericidal activity in vitro in the presence of complement and the protection in mice, since a bactericidal IgG3 did not elicit protection. In addition, no significant difference in protective capacity was observed with bactericidal and nonbactericidal IgG1 antibodies, indicating that bactericidal activity is not a requirement for protection mediated by certain anti-lipooligosaccharide A antibodies. A reduction in protection in C5-deficient mice was observed, suggesting a significant role for complement in certain host defense mechanisms against B. pertussis infection.
Shahin
R D
RD
Laboratory of Pertussis, Food and Drug Administration, Bethesda, Maryland 20892.
Hamel
J
J
Leef
M F
MF
Brodeur
B R
BR
D000818:Animals; D000907:Antibodies, Bacterial / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D001886:Bordetella pertussis / Q000276:immunology* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology*; D008168:Lung / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D014132:Trachea / Q000382:microbiology; D014917:Whooping Cough / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides