Murine endothelial cell line cells, F-2: interaction with leukocytes and cytokines production.
Flowcytometry demonstrated that murine endothelial cell line F-2 expresses MHC class I antigen, FcR II, Mac-1 and vascular cell adhesion molecule-1 (VCAM-1), but not intercellular adhesion molecule-1 (ICAM-1) and class II antigen. However, co-culturing with TNF-alpha for 24 hr resulted in the increased expression of ICAM-1, and the decreased expression of VCAM-1. IL-1 alpha and IFN-gamma exerted this regulatory effect on VCAM-1 but not on ICAM-1. T (Con A blast) and B (LPS blast) cells adhered to F-2 cells at almost equal levels, and the adhesion was enhanced 20 to 50% when the cells were precultured with TNF-alpha for 24 hr. The inhibition assay using either (anti-ICAM-1 + anti-LFA-1, lymphocyte function-associated antigen-1) or (anti-VCAM-1 + anti-VLA-4, very late antigen-4) mAbs demonstrated that the ICAM-1 system was utilized more preferentially by T than B blasts when F-2 cells were stimulated with TNF-alpha, and the VCAM-1 system was vice versa under the unstimulated and stimulated conditions. Granulocytes also adhered to F-2 cells, but no mAbs could inhibit the adhesion. Although F-2 cells produced a considerable amount of IL-6, GM-CSF and neutrophil chemotactic activity, a 24 hr incubation with TNF-alpha resulted in an increase of 12 fold in IL-6 and 3 fold in neutrophil chemotactic activity production.
Maruyama
H
H
Department of Immunobiology, Faculty of Medicine, Kyoto University, Japan.
Toda
K
K
Uno
K
K
Miyake
K
K
Matsushima
K
K
Yamamoto
K
K
Mori
J K
JK
Masuda
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis* / Q000502:physiology; D004727:Endothelium / Q000166:cytology* / Q000502:physiology; D005260:Female; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008819:Mice, Nude
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D016207:Cytokines