title: [The connection between insulin-dependent biosynthesis of cell membrane signal ATP with amiloride-sensitive Na+/H+-metabolism in human erythrocytes].
abstract: Plasma membrane signal ATP (psATP) was synthesized in response to the effect insulin (0.4 microgram/ml) in erythrocyte membranes, washed in 0.25 M sucrose and maintained in the mixture containing all the components required for aerobic phosphorylation. Biosynthesis of psATP was completely inhibited by amiloride at a concentration of 2 x 10(-3) M. Ouabain (0.5 x 10(-6) M) inhibited the triphosphate synthesis by 95%. Monensin (0.3 x 10(-6) M) activated ATP formation 2-fold approximately in erythrocyte membranes both stimulated by insulin and free of the hormone effect. Monensin, added into the incubation mixture simultaneously with ouabain, abolished its inhibitory effect on psATP synthesis. In absence of Na+ the psATP biosynthesis was not detected. Protonophore FCCP stimulated the biosynthesis at various concentrations from 10(-7) to 10(-3) M. These data indicated that biosynthesis of psATP is related to Na+/H+ metabolism in plasma membranes of human erythrocytes.
authors.forename: A A
authors.initials: AA
authors.lastname: Karelin
authors.forename: V S
authors.initials: VS
authors.lastname: Demidova
authors.forename: A I
authors.initials: AI
authors.lastname: Marchuk
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000584:Amiloride / Q000494:pharmacology*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*
chemical_list: D007328:Insulin; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000584:Amiloride; D006859:Hydrogen; D000255:Adenosine Triphosphate; D012964:Sodium
vernacular_title: Sviaz' insulinstimuliruemogo biosinteza plazmomembrannogo signal'nogo ATF s amiloridchuvstvitel'nym Na+/H+-obmenom v éritrotsitakh cheloveka.