Participation of ouabainlike compound in reduced renal mass-saline hypertension.
We examined the role of ouabainlike compound in reduced renal mass-saline hypertension using a population of rats immunized with ouabain. To develop ouabain-immunized rats, ouabain-bovine serum albumin conjugates were injected subcutaneously three times at 4-week intervals. Titer determinations were made 2 weeks after the third immunization, and rats with high titers were used in the study. Immunoglobulin G fractions from ouabain-immunized rats effectively inhibited the contractile response of guinea pig aorta to exogenous ouabain (150 nmol). Fourteen ouabain-immunized and seven nonimmunized control rats underwent subtotal nephrectomy. An additional eight ouabain-immunized and six nonimmunized rats served as sham-operated rats. Four groups of rats drank 1% NaCl solution for 3 weeks, and systolic blood pressure was measured weekly by the tail-cuff method. Two groups of sham-operated rats remained normotensive. In contrast, two groups of subtotally nephrectomized rats developed hypertension. However, among these rats, systolic blood pressure was significantly lower in ouabain-immunized rats than in nonimmunized rats (161 +/- 5 versus 180 +/- 3 [+/- SEM) mm Hg, P < .01). The decrease in blood pressure was accompanied by a significant inhibition of aortic hypertrophy (P < .05). These results indicate that chronic blockade of circulating ouabainlike compound partly ameliorates reduced renal mass-saline hypertension and suggest that circulating ouabainlike compound may be involved in the pathophysiology in this model of hypertension.
Yamada
K
K
Sanraku Hospital, Tokyo, Japan.
Goto
A
A
Nagoshi
H
H
Hui
C
C
Yagi
N
N
Sasabe
M
M
Omata
M
M
D000311:Adrenal Glands / Q000033:anatomy & histology; D000704:Analysis of Variance; D000818:Animals; D001011:Aorta / Q000033:anatomy & histology; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology; D015551:Autoimmunity; D001794:Blood Pressure; D001835:Body Weight; D003404:Creatinine / Q000097:blood; D006168:Guinea Pigs; D006321:Heart / Q000033:anatomy & histology; D006400:Hematocrit; D006977:Hypertension, Renal / Q000097:blood / Q000503:physiopathology*; D007074:Immunoglobulin G / Q000494:pharmacology; D066298:In Vitro Techniques; D007668:Kidney / Q000033:anatomy & histology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D009392:Nephrectomy; D009929:Organ Size; D010042:Ouabain / Q000276:immunology* / Q000378:metabolism*; D011188:Potassium / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000097:blood; D012964:Sodium / Q000097:blood
D007074:Immunoglobulin G; D010042:Ouabain; D012964:Sodium; D003404:Creatinine; D012083:Renin; D011188:Potassium