The regional hydrolysis of ethyl acrylate to acrylic acid in the rat nasal cavity.
Cytotoxicity is primarily limited to the olfactory epithelium of the dorsal meatus region of the nasal cavity of rodents following inhalation exposure to acrylic monomers. To investigate the biochemical basis for this effect, three regions of the Fischer F344N rat nasal cavity were evaluated for carboxylesterase activity for the representative acrylic ester, ethyl acrylate. Prior studies have indicated that the rodent olfactory epithelium is sensitive to the cytotoxic effects of short chain organic acids. In this study, no regional difference in carboxylesterase activity was observed between sensitive and non-sensitive regions of olfactory epithelium. Respiratory epithelium (resistant to cytotoxicity) was found to be have a much lower rate of carboxylesterase activity than olfactory epithelium. These results suggest that the regional distribution of cytotoxicity observed in the rat nasal cavity at high concentrations of inhaled acrylic monomers may be due in part to the amount of released organic acid following deposition. However, the observation of the same esterase activity in sensitive and nonsensitive olfactory regions suggests that nasal air flow patterns and regional deposition may also be critical factors.
Frederick
C B
CB
Rohm and Haas Co., Spring House, PA 19477-0904.
Udinsky
J R
JR
Finch
L
L
D000179:Acrylates / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity*; D000280:Administration, Inhalation; D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008297:Male; D009296:Nasal Cavity / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009831:Olfactory Mucosa / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344
D000179:Acrylates; C040833:ethyl acrylate; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C036658:acrylic acid