title: [Inhibitory effect of portal pooling, bacterial translocation, and Kupffer cell activation on hepatic regeneration after partial hepatectomy by repeated portal triad cross clamping in rats].
abstract: Effect of hepatic ischemia and reperfusion on hepatic regeneration after 70% partial hepatectomy was evaluated in rats. Total hepatic ischemia by portal triad cross clamping (15 minutes) and reperfusion (15 minutes) was repeated two times during partial hepatectomy in the PS, non-PS, and G groups. In the C group, partial hepatectomy was made as a control without ischemia and reperfusion. In order to evaluate the effect of portal pooling, portal systemic shunt (PS shunt) was made by splenic transposition to subcutaneous space in the PS group, and compared with the non-PS group. Gadolinium chloride (GdCl3), the selective blocker of Kupffer (K) cell, was intravenously administered to the rat in the G group. Hepatic regeneration rates, labelling index of liver cells, rates of bacterial infection of mesenteric lymph nodes (MLN), blood levels of endotoxin (Ex) and tumor necrosis factor-alpha (TNF) were compared. Hepatic regeneration at 28 days was suppressed by total hepatic ischemia in the non-PS group. Increased positive rates of MLN culture and blood levels of Ex showed bacterial translocation induced by the portal pooling during portal triad clamping. PS shunt reduced both bacterial translocation and the suppression of hepatic regeneration occurred in the PS group. Hepatic regeneration was not suppressed and blood TNF level did not increased in the G group by the inhibition of K cell function. In conclusion, repeated total hepatic ischemia and reperfusion induced portal pooling, bacterial translocation, and activated K cell, then inhibited hepatic regeneration after partial hepatectomy.
authors.affiliation: First Department of Surgery, Kobe University School of Medicine.
authors.forename: K
authors.initials: K
authors.lastname: Furuchi
authors.forename: M
authors.initials: M
authors.lastname: Usami
authors.forename: H
authors.initials: H
authors.lastname: Ohyanagi
authors.forename: Y
authors.initials: Y
authors.lastname: Saitoh
mesh_terms: D000818:Animals; D018407:Bacterial Physiological Phenomena; D003250:Constriction; D006498:Hepatectomy / Q000379:methods; D007511:Ischemia / Q000503:physiopathology; D007728:Kupffer Cells / Q000276:immunology*; D008099:Liver / Q000098:blood supply / Q000382:microbiology*; D008115:Liver Regeneration; D008262:Macrophage Activation; D008297:Male; D011169:Portal Vein / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion