Hydroxyl radical generation and membrane fluidity of erythrocytes treated with lipopolysaccharide.
The effect of lipopolysaccharide (LPS) and/or bile acids on rat erythrocyte membranes was studied in vitro. Addition of LPS isolated from E. coli (J5 mutant) into the erythrocyte resulted in the decrease of membrane fluidity as determined by spin labelling using electron paramagnetic resonance (EPR). This was accompanied by membrane fragility. It was found that hydroxyl radicals were generated from erythrocytes treated with LPS by using DMPO spin trapping. However, pretreatment of erythrocytes with taurine-conjugated bile acids was found to modify the membrane response induced by LPS. Taurocholic acid (TCA) and tauroursodeoxycholic acid (TUDCA) prevented the decrease of membrane fluidity induced by LPS, and, as a result, the membrane integrity was maintained although no significant changes were observed in the amount of hydroxyl radicals produced by LPS addition. However, taurochenodeoxycholic acid (TCDCA) exhibited little beneficial effect on the dynamic properties and the function of the erythrocyte membranes, although the hydroxyl radical declined markedly in the erythrocytes. Therefore, it is suggested that TCA and TUDCA have a protective effect against LPS-induced membrane fragility by modulating membrane fluidity.
Hino
Y
Y
Second Department of Medicine, Kurume University School of Medicine, Japan.
Kumashiro
R
R
Sata
M
M
Nishi
J
J
Ogura
R
R
Tanikawa
K
K
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects*; D005609:Free Radicals; D006878:Hydroxides / Q000097:blood* / Q000737:chemistry; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008560:Membrane Fluidity; D051381:Rats; D017208:Rats, Wistar
D001647:Bile Acids and Salts; D005609:Free Radicals; D006878:Hydroxides; D008070:Lipopolysaccharides