Isolation of Trichoderma reesei genes highly expressed on glucose-containing media: characterization of the tef1 gene encoding translation elongation factor 1 alpha.
Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1 alpha (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85%. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly15 codon.
Nakari
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VTT Biotechnical Laboratory, Espoo, Finland.
Alatalo
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Penttilä
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003470:Culture Media; D018076:DNA, Complementary; D005091:Exons; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism*; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D010735:Phosphoglycerate Kinase / Q000235:genetics; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D018076:DNA, Complementary; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010735:Phosphoglycerate Kinase; D005947:Glucose