title: Exogenous myristic acid can be partially degraded prior to activation to form acyl-acyl carrier protein intermediates and lipid A in Vibrio harveyi.
abstract: To study the involvement of acyl carrier protein (ACP) in the metabolism of exogenous fatty acids in Vibrio harveyi, cultures were incubated in minimal medium with [9,10-3H]myristic acid, and labeled proteins were analyzed by gel electrophoresis. Labeled acyl-ACP was positively identified by immunoprecipitation with anti-V. harveyi ACP serum and comigration with acyl-ACP standards and [3H]beta-alanine-labeled bands on both sodium dodecyl sulfate- and urea-polyacrylamide gels. Surprisingly, most of the acyl-ACP label corresponded to fatty acid chain lengths of less than 14 carbons: C14, C12, C10, and C8 represented 33, 40, 14, and 8% of total [3H]14:0-derived acyl-ACPs, respectively, in a dark mutant (M17) of V. harveyi which lacks myristoyl-ACP esterase activity; however, labeled 14:0-ACP was absent in the wild-type strain. 14:0- and 12:0-ACP were also the predominant species labeled in complex medium. In contrast, short-chain acyl-ACPs (< or = C6) were the major labeled derivatives when V. harveyi was incubated with [3H]acetate, indicating that acyl-ACP labeling with [3H]14:0 in vivo is not due to the total degradation of [3H]14:0 to [3H]acetyl coenzyme A followed by resynthesis. Cerulenin increased the mass of medium- to long-chain acyl-ACPs (> or = C8) labeled with [3H]beta-alanine fivefold, while total incorporation of [3H]14:0 was not affected, although a shift to shorter chain lengths was noted. Additional bands which comigrated with acyl-ACP on sodium dodecyl sulfate gels were identified as lipopolysaccharide by acid hydrolysis and thin-layer chromatography. The levels of incorporation of [3H] 14:0 into acyl-ACP and lipopolysaccharide were 2 and 15%, respectively, of that into phospholipid by 10 min. Our results indicate that in contrast to the situation in Escherichia coli, exogenous fatty acids can be activated to acyl-ACP intermediates after partial degradation in V. harveyi and can effectively label products (i.e., lipid A) that require ACP as an acyl donor.
authors.affiliation: Atlantic Research Centre, Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Z
authors.initials: Z
authors.lastname: Shen
authors.forename: D M
authors.initials: DM
authors.lastname: Byers
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D000213:Acyl Carrier Protein / Q000031:analogs & derivatives* / Q000378:metabolism*; D000215:Acylation; D002569:Cerulenin / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D008050:Lipid A / Q000378:metabolism; D008954:Models, Biological; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D014733:Vibrio / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000213:Acyl Carrier Protein; D005227:Fatty Acids; D008050:Lipid A; D009227:Myristic Acids; D019814:Myristic Acid; D002569:Cerulenin; D000105:Acetyl Coenzyme A