title: Coactosin, a 17 kDa F-actin binding protein from Dictyostelium discoideum.
abstract: A 17 kDa protein, designated as coactosin, has been purified from an actin-myosin complex reconstituted in vitro from a soluble fraction of Dictyostelium discoideum cells. The protein binds to F-actin in vitro without significantly altering its viscosity. Immunoblots labeled with monoclonal antibodies indicate that part of the protein is associated with the detergent-insoluble cytoskeleton. cDNA clones comprising the entire coding region of coactosin have been isolated from an expression library. The cDNA-derived amino-acid sequence reveals similarities of coactosin to the drebrins identified in neurons and to actin-binding proteins from other organisms, including yeast ABP1p, and yeast and vertebrate cofilins.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: E L
authors.initials: EL
authors.lastname: de Hostos
authors.forename: B
authors.initials: B
authors.lastname: Bradtke
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: G
authors.initials: G
authors.lastname: Gerisch
mesh_terms: D000205:Actomyosin / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016477:Artifacts; D001483:Base Sequence; D003593:Cytoplasm / Q000737:chemistry; D018076:DNA, Complementary / Q000276:immunology; D004023:Dictyostelium / Q000737:chemistry* / Q000235:genetics; D005404:Fixatives / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D015800:Protozoan Proteins
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D005404:Fixatives; D005656:Fungal Proteins; D008840:Microfilament Proteins; D015800:Protozoan Proteins; C085568:coactosin protein, Dictyostelium discoideum; D000205:Actomyosin