[Immunohistological grade of renal cell carcinoma employing anti Leu-M1 antibody and anti EMA antibody].
Attempting to establish more objective grading system of renal cell carcinoma, I studied 104 renal cell carcinoma tissue samples from 52 cases by immunohistochemical technique employing the anti-Leu M1 antibody and the anti-EMA antibody. These 52 cases could be classified into 4 groups according to the reactivity for Leu-M1 (L) and EMA (E), i.e., L+E-, L+E+, L-E+, and L-E-. Considering the expression pattern of Leu-M1 (L) and EMA (E) in normal adult/fetal renal tissue, I speculated that normal cellular characters of the proximal tubules were preserved most well in the L+E- carcinomas, and gradually lost in the L+E+ carcinomas, L-E+ carcinomas, and L-E- carcinomas in this order. The reactions L+E-, L+E+, L-E+, and L-E- were designated immunohistochemical grade 1, 2, 3, and 4, respectively. In most cases, the immunohistochemical grade corresponded with the conventional histologic grade. However, dissociation of more than 2 steps of the grade was observed in some cases. I analyzed the cases with such dissociation and discussed usefulness of the immunohistochemical grading system (as a supplemental role for histologic grading) for renal cell carcinoma.
Senga
Y
Y
Department of Urology, Yokohama City University School of Medicine.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000401:mortality / Q000473:pathology*; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000401:mortality / Q000473:pathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D008875:Middle Aged; D018396:Mucin-1; D009367:Neoplasm Staging; D011379:Prognosis
D000906:Antibodies; D015214:Antigens, Differentiation, Myelomonocytic; D008562:Membrane Glycoproteins; D018396:Mucin-1