New method for detection of Candida albicans in human blood by polymerase chain reaction.
We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.
Miyakawa
Y
Y
Department of Microbiology, Yamanashi Medical College, Japan.
Mabuchi
T
T
Fukazawa
Y
Y
D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005069:Evaluation Studies as Topic; D016469:Fungemia / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004271:DNA, Fungal