Dietary nucleotides: effects on the gastrointestinal system in swine.
Nucleotides in the intestinal lumen may decrease the inflammatory response to ischemia-reperfusion. In a newborn-swine model, we showed that perfusion of the intestinal lumen with nucleotides in concentrations similar to those in human milk induced hyperemia. The levels of hypoxanthine (and xanthine) were not increased in the presence of nucleotides during ischemia-reperfusion, and the number of leukocytes accumulated in the intestine was reduced in the presence of nucleotides. Furthermore, nucleotides may have decreased protein leak and the production of nitric oxide during ischemia. These effects are not changed significantly in the presence of an adenosine antagonist. We interpreted our results to indicate that the protective effects of nucleotides in the intestinal lumen are not due to adenosine alone.
Bustamante
S A
SA
Department of Pediatrics, Louisiana State University School of Medicine, New Orleans 70112.
Sanches
N
N
Crosier
J
J
Miranda
D
D
Colombo
G
G
Miller
M J
MJ
D000241:Adenosine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D004247:DNA / Q000096:biosynthesis; D004032:Diet; D004064:Digestive System / Q000187:drug effects*; D006098:Granulocytes; D006940:Hyperemia / Q000139:chemically induced; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007413:Intestinal Mucosa / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007511:Ischemia / Q000378:metabolism / Q000473:pathology; D007958:Leukocyte Count; D008871:Microvilli / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009711:Nucleotides / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D015427:Reperfusion Injury / Q000517:prevention & control; D013552:Swine
D007042:Hypoxanthines; D009573:Nitrites; D009711:Nucleotides; D011506:Proteins; D019271:Hypoxanthine; D004247:DNA; D000241:Adenosine