[Fast identification of Salmonella by chemiluminescent photographic detection].
Salmonella anatis was identified by the chemiluminescent photographic detection technique established in this lab. The horseradish peroxidase labelled antibody was incubated with the bacteria immobilized on nitrocellulose film; after washing, the film was contacted with a filter paper with absorbed chemiluminescence substrate solution. Twenty minutes of exposure of the chemiluminescence produced to X-ray film gave the positive results. As few as one hundred bacteria could be detected in about 2h. The tests showed that the specificity of the method is good.
Yang
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Wu
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Zhang
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Zhang
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Y
D008163:Luminescent Measurements; D010781:Photography / Q000379:methods; D012475:Salmonella / Q000145:classification / Q000302:isolation & purification*; D012680:Sensitivity and Specificity