title: Crystallization and preliminary crystallographic studies of Saccharomyces cerevisiae alcohol dehydrogenase I.
abstract: The cytoplasmic yeast alcohol dehydrogenase I crystallized at 5 degrees C as hexagonal plates or short columns in the presence of NAD+ and 2,2,2-trifluoroethanol, in sodium N-tris(hydroxymethyl)methyl-3-aminopropanesulfonate buffer at pH 8.2 to 8.6, using polyethylene glycol 4000 as precipitant. X-ray diffraction data to 3.2 A resolution show that the crystals are hexagonal in space group P622 with unit cell dimensions a = b = 147.9 A, c = 69.1 A. There is one subunit of the tetrameric enzyme per asymmetric unit, giving a packing density of 2.9 A3/Da.
authors.affiliation: Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: S
authors.initials: S
authors.lastname: Ramaswamy
authors.forename: D A
authors.initials: DA
authors.lastname: Kratzer
authors.forename: A D
authors.initials: AD
authors.lastname: Hershey
authors.forename: P H
authors.initials: PH
authors.lastname: Rogers
authors.forename: A
authors.initials: A
authors.lastname: Arnone
authors.forename: H
authors.initials: H
authors.lastname: Eklund
authors.forename: B V
authors.initials: BV
authors.lastname: Plapp
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D000426:Alcohol Dehydrogenase