Electron microscopic analysis of the normal and the activated pleural macrophage.
Despite an apparent role in pleural pathophysiology, little information is known about pleural macrophage morphology. Intrapleural tetracycline (TCN) results in pleural macrophage influx and pleural fibrosis; intrapleural carrageenan (CAR) induces macrophage influx without ensuing fibrosis. Pleural macrophages collected from normal (NL) and TCN- or CAR-exposed rabbit pleural spaces were examined with electron microscopy. Cellular size; number of microvilli; pseudopods; coated pits (CP) and coated vesicles (CV); and prevalence of golgi, rough endoplasmic reticulum (RER), and intermediate filaments (IF) were determined. The means of each variable in each group were assessed by one-way analysis of variance, with post hoc testing performed by Scheffe F test; p < or = .05 was considered significant. TCN-stimulated pleural macrophages were characterized by their small perimeters. CAR-induced pleural macrophages were marked by their large size and abundant intracellular amorphous material. They had larger perimeters, areas, and diameters than the TCN-induced or normal macrophages and thus smaller numbers of CV + CP per area. The normal pleural macrophages were characterized by more IF, microvilli, and microvilli per perimeter than either the CAR- or TCN-induced pleural macrophages. No differences between groups were found in nuclear cytoplasmic ratios, number of pseudopods, and content of golgi or of RER. The results suggest that normal pleural macrophages and TCN- and CAR-induced pleural macrophages differ morphologically and that these morphologic differences reflect functional differences.
Baumann
M H
MH
Division of Pulmonary and Critical Care Medicine, Medical University of South Carolina, Charleston 29425.
Heinrich
K
K
Sahn
S A
SA
Green
C
C
Harley
R
R
Strange
C
C
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D007267:Injections; D007382:Intermediate Filaments / Q000648:ultrastructure; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010994:Pleura / Q000166:cytology*; D011817:Rabbits; D012016:Reference Values; D013752:Tetracycline / Q000494:pharmacology
D002351:Carrageenan; D013752:Tetracycline