Uptake and distribution of intravenously or intravesically administered photosensitizers in the rat.
Photodynamic therapy using i.v. injected porphyrin photosensitizers have been used to treat selected cases of superficial bladder cancer. Since cutaneous photosensitivity, lasting 6-8 weeks, is a well known undesirable side effect of this therapy, we instilled the photosensitizers intravesically in rats and compared the uptake of photosensitizers in different tissues by this route of administration with the uptake after intravenous injection. The intravesical mode of delivery enhanced photosensitizer uptake in the bladder wall, while giving low concentrations in extravesical organs. Intravesical instillation of the photosensitizers may therefore increase their efficacy and reduce phototoxicity as compared with intravenous injection. Comparing the results obtained by two assays, one based on porphyrin fluorescence and the other based on the application of radioactively labelled photosensitizers, it was concluded that the i.v. administration route may result in tissue uptake of significant amounts of aggregated non-fluorescent, supposedly inactive drug, while the intravesical administration led to less uptake of aggregates relative to active drug monomers.
Windahl
T
T
Department of Urology, Orebro Medical Center Hospital, Sweden.
Peng
Q
Q
Moan
J
J
Hellsten
S
S
Axelsson
B
B
Löfgren
L
L
D000818:Animals; D017323:Dihematoporphyrin Ether / Q000378:metabolism; D005260:Female; D006415:Hematoporphyrins / Q000378:metabolism*; D007275:Injections, Intravenous; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D001743:Urinary Bladder / Q000378:metabolism
D006415:Hematoporphyrins; D017323:Dihematoporphyrin Ether