Amelioration of antioxidant enzyme suppression and proteinuria in cyclosporin-treated puromycin nephrosis.
The effect of cyclosporin (CS) on intrinsic glomerular level of antioxidants in puromycin aminonucleoside (PAN) nephrosis was examined. A single intravenous dose of PAN (50 mg/kg body weight) given to Sprague-Dawley rats resulted in marked proteinuria. Ten days after PAN injection, the rats were treated with daily intraperitoneal injection of CS (10 mg/kg body weight/day) for 10 days. PAN-treated rats without CS treatment (PAN rats) had significantly lower activities of glomerular superoxide dismutase (SOD) and catalase (CAT) than normal rats (p < 0.05, respectively). When compared with PAN rats, CS-treated PAN rats had significantly less proteinuria and higher activities of glomerular SOD and CAT (p < 0.01 and p < 0.05, respectively). Significant elevation of glomerular malondialdehyde (MDA) level characteristic of PAN rats was absent in CS-treated PAN rats. Moreover, segmental sclerosis with capsular adhesion, hyalinosis, epithelial cell foot process fusion and microvillous transformation seen in PAN rats were apparently attenuated in CS-treated PAN rats. When compared with normal rats, rats receiving CS only had a significantly higher CAT activity and MDA level (p < 0.01 and p < 0.05, respectively). Assessment of glomerular reduced glutathione revealed no significant differences among PAN rats, CS-treated PAN rats, normal rats, and rats receiving only CS. These data indicate that glomerular antioxidant enzyme activities are modulated by CS.
Wang
J S
JS
Department of Pathology, Veterans General Hospital-Taipei VACRS, Taiwan, ROC.
Yang
A H
AH
Chen
S M
SM
Young
T K
TK
Chiang
H
H
Liu
H C
HC
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D016572:Cyclosporine / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009401:Nephrosis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D011507:Proteinuria / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D011692:Puromycin Aminonucleoside / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism*
D008315:Malondialdehyde; D011692:Puromycin Aminonucleoside; D016572:Cyclosporine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione