title: Permeation of human chorioamniotic membranes by Escherichia coli in vitro.
abstract: OBJECTIVE
  Our goal was to study the permeation of Escherichia coli through human chorioamniotic membranes in vitro.
  
  
  METHODS
  Medium was placed in two compartments separated by chorioamniotic membranes obtained from six cesarean sections at term. The compartment faced by the chorion was inoculated with E. coli. Both compartments were sampled over 12 hours for observation of bacterial growth. Controls were performed without membranes.
  
  
  RESULTS
  In the compartment that was inoculated, concentration of E. coli increased from 10(6) to 10(10) colony-forming units per milliliter. In the compartment faced by amnion, bacterial growth was observed after 6 hours and reached 10(3) colony-forming units per milliliter. Permeation of E. coli was confirmed histopathologically. The change of glucose and lactate was linear. In the controls the concentration of E. coli increased to 10(7) (p < 0.001).
  
  
  CONCLUSIONS
  E. coli organisms permeate viable chorioamniotic membranes. The membranes constitute a weak barrier against ascending infection and do not inhibit bacterial growth.
authors.affiliation: Department of Obstetrics and Gynecology, University of Bern, Switzerland.
authors.forename: T N
authors.initials: TN
authors.lastname: Gyr
authors.forename: A
authors.initials: A
authors.lastname: Malek
authors.forename: F
authors.initials: F
authors.lastname: Mathez-Loic
authors.forename: H J
authors.initials: HJ
authors.lastname: Altermatt
authors.forename: T
authors.initials: T
authors.lastname: Bodmer
authors.forename: K
authors.initials: K
authors.lastname: Nicolaides
authors.forename: H
authors.initials: H
authors.lastname: Schneider
mesh_terms: D000650:Amnion / Q000378:metabolism / Q000382:microbiology*; D002021:Buffers; D002245:Carbon Dioxide / Q000493:pharmacokinetics; D002823:Chorion / Q000378:metabolism / Q000382:microbiology*; D046508:Culture Techniques; D005260:Female; D005947:Glucose / Q000493:pharmacokinetics; D006801:Humans; D007773:Lactates / Q000493:pharmacokinetics; D019344:Lactic Acid; D008431:Maternal-Fetal Exchange; D010100:Oxygen / Q000493:pharmacokinetics; D010539:Permeability; D011188:Potassium / Q000493:pharmacokinetics; D011247:Pregnancy; D012044:Regression Analysis; D012964:Sodium / Q000493:pharmacokinetics; D013997:Time Factors
chemical_list: D002021:Buffers; D007773:Lactates; D002245:Carbon Dioxide; D019344:Lactic Acid; D012964:Sodium; D005947:Glucose; D011188:Potassium; D010100:Oxygen