Cryopreservation of Angiostrongylus cantonensis third-stage larvae.
An optimum protocol for the cryopreservation of third-stage larvae (L3) of Angiostrongylus cantonensis was determined by: (i) use of different dimethyl sulphoxide (DMSO) concentrations; (ii) use of different FBS concentrations in RPMI-1640 medium; and (iii) variation in the thawing temperature. Studies showed that with concentrations of DMSO at 4.8% and FBS at 40% and a thawing temperature of 43 degrees C, L3 survival assessed by motility was highest at 56%. The infectivity of the cryopreserved L3 of A. cantonensis was 20% compared to controls obtained from slugs.
Mahajan
R K
RK
Department of Zoonosis, Haffkine Institute, Parel, Bombay, India.
Renapurkar
D M
DM
D000801:Angiostrongylus / Q000302:isolation & purification / Q000472:pathogenicity; D000818:Animals; D001769:Blood; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D004121:Dimethyl Sulfoxide; D007814:Larva / Q000472:pathogenicity; D008974:Mollusca; D051381:Rats; D013696:Temperature
D003470:Culture Media; D004121:Dimethyl Sulfoxide