title: Chelate root filling cements: biological properties.
abstract: The purpose of this study was to test in vivo and in vitro the toxicity and the antibacterial activity of an experimental chelate cement (HN cement) using zinc oxide-eugenol cement as a reference. After subcutaneous injection of the spatulated HN cement paste in rats, it induced markedly less tissue injuries than did the zinc oxide-eugenol cement. In toxicity tests using cultures of human fibroblasts, the HN cement was found to be less toxic than the reference cement. Bacteriologically, blood agar plates inoculated with Streptococcus sanguis, Staphylococcus aureus, and the anaerobic Prevotella intermedia developed inhibition zones between 3 and 12 mm upon application of both cements. Both demonstrated marked bacteriostatic and bactericidal properties.
authors.affiliation: Department of Dental Material, Royal Dental College, Arhus, Denmark.
authors.forename: T H
authors.initials: TH
authors.lastname: Nielsen
authors.forename: D
authors.initials: D
authors.lastname: Arenholt-Bindslev
authors.forename: M
authors.initials: M
authors.lastname: Kilian
authors.forename: H P
authors.initials: HP
authors.lastname: Philipsen
mesh_terms: D000818:Animals; D001439:Bacteroides / Q000187:drug effects; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D003738:Dental Cements / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007659:Ketones / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D008297:Male; D008826:Microbial Sensitivity Tests; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D013211:Staphylococcus aureus / Q000187:drug effects; D013298:Streptococcus sanguis / Q000187:drug effects; D015032:Zinc / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D002614:Chelating Agents; D003738:Dental Cements; C080676:HN cement; D007659:Ketones; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; D015032:Zinc; D015034:Zinc Oxide