title: [Comparison between the effects of (-)- and (+)-gossypol on protein kinase C and protein kinase A].
abstract: Protein kinase C(PKC) and protein kinase A(PKA) purified from rat liver were incubated with (-)- and (+)-gossypol. The activity of PKC was significantly inhibited in a concentration-dependent manner by both (-)-gossypol and (+)-gossypol. (-)-gossypol was found to inhibit type I PKA, whereas the inhibitory action of (+)-gossypol for type I PKA was less potent. Both (-)-gossypol and (+)-gossypol showed inhibition for type II PKA only at high concentration. These results suggest that administration of gossypol might impair cellular signal transduction.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
authors.forename: D M
authors.initials: DM
authors.lastname: Xiao
authors.forename: W H
authors.initials: WH
authors.lastname: Zhou
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Zhu
authors.forename: J B
authors.initials: JB
authors.lastname: Zhang
authors.forename: G D
authors.initials: GD
authors.lastname: Yang
authors.forename: H C
authors.initials: HC
authors.lastname: Chen
mesh_terms: D000818:Animals; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006072:Gossypol / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry; D008297:Male; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
chemical_list: D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D006072:Gossypol