title: Phase-selective AC adsorptive stripping voltammetric assay for aminopterin and 10-Edam in human serum.
abstract: Aminopterin was studied as a model compound for its analogues which maintain the pteridine ring in their structure. Its adsorptive behaviour on mercury was studied and the DC adsorptive stripping and phase-selective AC adsorptive stripping conditions were optimized. 10-Edam, an aminopterin analogue, was studied and shown to behave similarly to aminopterin. Phase-selective AC voltammetry provided the best signal and gave a detection limit of 4 x 10(-12) M aminopterin in aqueous solution employing an accumulation time of 10 min. The optimized method was applied to the analysis of both aminopterin and 10-Edam respectively in human serum. After extraction with a C18 reversed-phase cartridge the detection limit of the method was 1 x 10(-8) M aminopterin and the overall assay percentage recovery was 73.5% (n = 5) at a concentration of 5 x 10(-7) M aminopterin in serum. The analysis of 10-Edam at the same concentration in serum yielded the higher percentage recovery of 94.46% (n = 5) following the same procedure.
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Asturias, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Malone
authors.forename: A
authors.initials: A
authors.lastname: Costa Garcia
authors.forename: P
authors.initials: P
authors.lastname: Tunon Blanco
authors.forename: M R
authors.initials: MR
authors.lastname: Smyth
mesh_terms: D000327:Adsorption; D000630:Aminopterin / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006801:Humans; D008628:Mercury
chemical_list: D008628:Mercury; C040210:edatrexate; D000630:Aminopterin