A monoclonal antibody that recognizes HLA-B27 in the context of peptides.
The T2 mutant cell line is unable to load peptides into the MHC class I Ags inside the cells. These "empty" MHC class I Ags are not expressed on the cell surface unless the cells are cultured at low temperatures. Expression will occur at 37 degrees C only in the presence of peptides that bind to and stabilize the class I Ags. T2 cells transfected with the B*2705 gene were tested with a panel of anti-HLA-B27 mAb. Two of the antibodies, ME1 and KS3, reacted with the "empty" HLA-B27 expressed at low culture temperatures. Three antibodies, B27.M1, B27.M2, and Ye-2, were unreactive with these "empty" HLA-B27. The cells were then incubated with a panel of HLA-B27-binding peptides. One of the antibodies, Ye-2, became reactive when the cells were incubated with a peptide derived from HIV gp120 and to a less degree with a peptide derived from histone H3.3. Mouse L cells transfected with the B*2705 and the human beta 2m genes also reacted very poorly with B27.M1, B27.M2, and Ye-2. Those two peptides were also able to induce high increase in Ye-2 reactivity. Alternately, increase in Ye-2 reactivity was also observed when the L cells were incubated with IFN-gamma or TNF-alpha. These experiments indicate that the Ye-2 anti-HLA-B27 mAb recognizes HLA-B27 in the context of certain residing peptides either added exogenously or expressed endogenously. The B27.M1 and B27.M2 antibodies might share similar characteristics.
Wang
J
J
Department of Medicine, University of California Los Angeles 90024.
Yu
D T
DT
Fukazawa
T
T
Kellner
H
H
Wen
J
J
Cheng
X K
XK
Roth
G
G
Williams
K M
KM
Raybourne
R B
RB
D000595:Amino Acid Sequence; D000938:Antigen-Presenting Cells / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D020126:Brefeldin A; D003517:Cyclopentanes / Q000494:pharmacology; D015796:HLA-B27 Antigen / Q000276:immunology*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000276:immunology; D006657:Histones / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins; D013696:Temperature; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000956:Antigens, Viral; D003517:Cyclopentanes; D015796:HLA-B27 Antigen; D006360:Heat-Shock Proteins; D006657:Histones; D010455:Peptides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D020126:Brefeldin A; D007371:Interferon-gamma