title: Fluorescent in situ hybridization (FISH): a new application in the delineation of true vs. pseudomosaicism in prenatal diagnosis.
abstract: Metaphase chromosomes and interphase nuclei from nine amniotic fluid cultures were studied with fluorescence in situ hybridization (FISH). The samples were initially analyzed with routine G-banding and were diagnosed as having true mosaicism (five patients) or pseudomosaicism (four patients). In our study, FISH analysis could provide additional information to distinguish pseudo- from true mosaicism by allowing interphase studies and analysis of an increased number of metaphase spreads. These results suggest a multilinear origin of 'in situ' colonies of cells.
authors.affiliation: Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore.
authors.forename: S
authors.initials: S
authors.lastname: Schwartz
authors.forename: J
authors.initials: J
authors.lastname: Leana-Cox
mesh_terms: D000653:Amniotic Fluid / Q000502:physiology; D001399:Azure Stains; D002871:Chromosome Banding; D015342:DNA Probes; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase / Q000235:genetics; D009030:Mosaicism; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*
chemical_list: D001399:Azure Stains; D015342:DNA Probes