title: Antibody response to a synthetic peptide derived from the human papillomavirus type 6/11 L2 protein in recurrent respiratory papillomatosis: correlation between Southern blot hybridization, polymerase chain reaction, and serology.
abstract: Recurrent respiratory papillomatosis (RRP) is the most common benign tumour of the larynx, affecting both children and adults. We present a series of 25 patients, including 10 cases of juvenile multiple, 8 cases of adult solitary, and 7 cases of adult multiple RRP. Biopsy tissue from each patient was screened by Southern blot hybridization and polymerase chain reaction for the presence of human papillomavirus (HPV) DNA. Sera from patients and age- and sex-matched controls were tested for the presence of HPV-specific antibodies using a synthetic peptide derived from the minor capsid protein (L2) of HPV 6/11. By Southern blot hybridization and/or polymerase chain reaction, biopsies from all patients were positive for HPV 6/11 DNA. There was no difference in antibody response between cases and controls. Female cases and controls had significantly higher antibody titers than male subjects. A correlation was observed between the HPV-specific antibody level and the number of surgery-necessitating recurrences.
authors.affiliation: Department of Experimental Virology, First Medical Faculty, Charles University, Prague, Czechoslovakia.
authors.forename: R
authors.initials: R
authors.lastname: Tachezy
authors.forename: E
authors.initials: E
authors.lastname: Hamsikova
authors.forename: J
authors.initials: J
authors.lastname: Valvoda
authors.forename: M
authors.initials: M
authors.lastname: Van Ranst
authors.forename: J
authors.initials: J
authors.lastname: Betka
authors.forename: R D
authors.initials: RD
authors.lastname: Burk
authors.forename: V
authors.initials: V
authors.lastname: Vonka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000032:analysis*; D015139:Blotting, Southern; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009364:Neoplasm Recurrence, Local; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D010212:Papilloma / Q000276:immunology / Q000821:virology*; D027383:Papillomaviridae / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012142:Respiratory Tract Neoplasms / Q000276:immunology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; C082746:L2 oncogene viral capsid protein, Human papillomavirus type 6; D009856:Oncogene Proteins, Viral; D010446:Peptide Fragments