Ferricenium salts instead of dioxygen in glucose oxidase catalysis. A direct interaction and analytical implications.
The ferricenium salt FcH+BF4- behaves as a specific substrate of glucose oxidase acting instead of dioxygen with the effective parameters of the Michaelis-Menten equation kcat = 2.60 x 10(2) s-1 and KM = 2.7 x 10(-4) M at 25 degrees C, pH 6.8 and [D-glucose] = 0.001 M. The activity of glucose oxidase can easily be monitored by conventional UV-vis spectrophotometry at 617 nm following the bleaching of the ferricenium dye.
Tegoulia
V
V
Division of Chemistry, G. V. Plekhanov Russian Economic Academy, Moscow.
Gnedenko
B B
BB
Ryabov
A D
AD
D002384:Catalysis; D005296:Ferrous Compounds / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000032:analysis / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D013379:Substrate Specificity
D005296:Ferrous Compounds; C064804:ferrocenium; D005949:Glucose Oxidase; D005947:Glucose; D010100:Oxygen