[Morphological changes in the supporting cells of the utricular macula due to streptomycin intoxication].
Morphological changes in cultured utricular supporting cells following streptomycin sulfate (SM) intoxication were investigated using an organ culture system. Utricles of guinea pig were exposed to 30 and 3mg/ml of SM for 1-3 days in culture. The number of lysosomes in the supporting cells increased daily, and mitochondria, myeloid bodies, granules and vesicles were observed within the lysosomes. As these components accumulated in the lysosomes, the number of granules and vesicles in the cytoplasma decreased. Acid phosphatase (AcPase) activity also decreased. After 1-3 days culture with SM, the culture medium was changed to a medium without SM. After removal of SM, the Golgi apparati appeared more developed and AcPase activity was higher. At the same time, lysosomes were markedly decreased in number and the endoplasmic reticulum showed a gradual reproduction. These findings suggest close relationships among the Golgi apparatus, lysosomes, secretory granules and the endplasmic reticulum.
Oda
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Y
Department of Otolaryngology, Hiroshima University School of Medicine.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000187:drug effects; D006056:Golgi Apparatus / Q000187:drug effects; D006168:Guinea Pigs; D007760:Labyrinth Supporting Cells / Q000166:cytology / Q000187:drug effects*; D008247:Lysosomes / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D012444:Saccule and Utricle / Q000166:cytology / Q000187:drug effects*; D013307:Streptomycin / Q000506:poisoning*
D000135:Acid Phosphatase; D013307:Streptomycin