title: Development of techniques for the isolation of iron from biological material for measurement of isotope ratios by fast atom bombardment mass spectrometry.
abstract: Stable isotopes have gained prominence in nutrition and trace element research. Fast atom bombardment mass spectrometry (FAB-MS) measurement of iron isotope ratios has an accuracy of > or equal to 99.7% with the stable isotopes (54)Fe, (56)Fe, and (58)Fe with relative standard deviations (RSDs) of < or equal to 0.9%. The isolation of iron from biological matrices can be accomplished in 4 h with almost total removal of isobaric interferences caused by (39)KOH, (39)K.H2O, (40)CaOH, and/or (40)Ca.H2O. FAB-MS isotope enrichment measurements from this method compare favorably to predicted absorption/enrichment levels.
authors.affiliation: Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: D R
authors.initials: DR
authors.lastname: Flory
authors.forename: L V
authors.initials: LV
authors.lastname: Miller
authors.forename: P V
authors.initials: PV
authors.lastname: Fennessey
mesh_terms: D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D007501:Iron / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification; D007503:Iron Isotopes; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
chemical_list: D007503:Iron Isotopes; D007501:Iron