title: Factors affecting circulating growth hormone binding protein in chickens.
abstract: Growth hormone binding protein (GHBP) may be an important factor in the regulation of growth and might provide an indirect, relatively noninvasive means of predicting the status of hepatic growth hormone receptor (GHR) activity. Several factors have been reported to influence growth hormone (GH), GHR, or GHBP. Therefore, these studies were conducted to test how age, sex, nutritional status, and glucocorticoids (cortisone acetate, CA) influence serum concentrations of chicken GHBP. Serum GHBP activity was highest (mean percentage specific binding (%SB) = 12.43 +/- .80) at hatch and decreased linearly (P < .0001) to 5 wk of age (%SB = 1.99 +/- 1.13). There were no sex-related differences in serum GHBP activity from hatch to 5 wk of age (P > .08). Short-term nutrient deprivation (24-h) of 4-wk-old broilers also had a significant effect on serum GHBP activity (P < .0001). Measurement of serum GHBP activity with refeeding (after a 24-h period without feed) restored %SB to normal values. Feeding broilers a low-protein diet (12% CP) did not significantly affect serum GHBP activity when compared with that of broilers fed a commercial broiler diet (23% CP; P > .30). Administration of cortisone (1, 5, and 10 mg/day), every 24 h for 7 days, had no effect, at any dose, on serum GHBP activity at 48 h and 1 wk after the last injection. These results indicate that serum GHBP activity is influenced by factors such as age and feed deprivation. It remains to be determined whether these changes in GHBP are associated with changes in GHR as reported for mammalian species.
authors.affiliation: Department of Animal Sciences, Oregon State University, Corvallis 97331.
authors.forename: E T
authors.initials: ET
authors.lastname: Tobar-Dupres
authors.forename: D P
authors.initials: DP
authors.lastname: Froman
authors.forename: S L
authors.initials: SL
authors.lastname: Davis
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000097:blood* / Q000187:drug effects; D002645:Chickens / Q000097:blood* / Q000254:growth & development; D003348:Cortisone / Q000008:administration & dosage*; D005260:Female; D005508:Food Deprivation; D008297:Male; D012727:Sex Characteristics; D015430:Weight Gain / Q000187:drug effects
chemical_list: D002352:Carrier Proteins; D003348:Cortisone; C051815:somatotropin-binding protein