Quantitative measurement of hemosiderin deposition in tissue sections of the liver by image analysis.
Hemosiderin has been quantitated in tissue sections by image analysis employing Perls' Berlin blue reaction. A linear correlation was observed between the mean optical density of the tissue section measured with a commercial image analyzer and the iron concentration measured with an atomic absorption spectrophotometer. The correlation coefficient of .945 (P < .001) indicated that a linear correlation existed between these measurements. Thus, the hemosiderin volume in tissue sections can be measured quantitatively by image analysis.
Masuda
T
T
Department of Pathology, School of Medicine, Iwate Medical University, Morioka, Japan.
Kasai
T
T
Satodate
R
R
D006485:Hemosiderin / Q000032:analysis*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D013054:Spectrophotometry, Atomic
D006485:Hemosiderin