Sulfonate-sulfur assimilation by yeasts resembles that of bacteria.
Three sulfonates were tested for their ability to serve as nutrients for Hansenula wingei, Rhodotorula glutinis, Trigonopsis variabilis and Saccharomyces cerevisiae. Cysteate, taurine and isethionate, under aerobic conditions, could be utilized as sources of sulfur, although in some instances final cell yields were less than those obtained with an equimolar amount of sulfate-sulfur. Sulfonate assimilation by S. cerevisiae resembled that of bacteria (reported earlier by us) in several aspects: first, sulfate-S was used in preference to that of sulfonate, when both were present; second, mutants unable to use sulfate as a source of sulfur because of deficiencies in ATP sulfurylase, adenylylsulfate kinase (APS kinase) or PAPS reductase were able to utilize sulfonates; and third, mutants deficient in sulfite reductase were unable to utilize sulfonates.
Uria-Nickelsen
M R
MR
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-2131.
Leadbetter
E R
ER
Godchaux
W
W
D000332:Aerobiosis; D003470:Culture Media; D003544:Cysteic Acid / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D007513:Isethionic Acid / Q000378:metabolism; D009154:Mutation; D013455:Sulfur / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism; D015003:Yeasts / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D013654:Taurine; D013455:Sulfur; D007513:Isethionic Acid; D003544:Cysteic Acid; D003545:Cysteine