Rapid and sensitive gas chromatographic determination of estazolam.
The triazolobenzodiazepine estazolam can be quantitated by gas chromatography with electron-capture detection. After addition of a suitable internal standard, plasma samples are extracted into toluene-isoamyl alcohol or benzene-isoamyl alcohol. The organic extract is separated, evaporated to dryness, reconstituted, and chromatographed using a 50:50 methyl-phenyl column (SP-2250). The sensitivity limit is approximately 1 ng of estazolam in a 1-ml sample. The method is suitable for clinical or experimental pharmacokinetic studies.
Kelly
J F
JF
Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Boston, MA 02111.
Greenblatt
D J
DJ
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D004949:Estazolam / Q000097:blood*; D008297:Male; D051379:Mice
D004949:Estazolam