title: A novel transcript from a pseudogene for human glucocerebrosidase in non-Gaucher disease cells.
abstract: Human glucocerebrosidase (GC)-encoding cDNA clones were isolated from a promyelocytic HL-60 cDNA library and analyzed. A novel cDNA clone was found to originate from a gene referred to as a GC pseudogene. Using the polymerase chain reaction (PCR) with primers specific for the GC pseudogene, we found that all the human cell lines examined, HL-60, K-562, WI-38, HepG2 and HeLa, expressed a pseudogene transcript. In vitro translation of RNA synthesized by transcription of the pseudogene cDNA produced a polypeptide of approximately 30 kDa.
authors.affiliation: Department of Biochemistry, Shimane Medical University, Izumo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Imai
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Asano
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
authors.forename: E I
authors.initials: EI
authors.lastname: Ginns
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005776:Gaucher Disease / Q000235:genetics; D005962:Glucosylceramidase / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011544:Pseudogenes
chemical_list: D018076:DNA, Complementary; D005962:Glucosylceramidase