PCR primed with VNTR core sequences yields species specific patterns and hypervariable probes.
The use of genomic DNA-based techniques in ecological and evolutionary studies has been limited by the availability of suitable probes for species of interest due to the technical difficulty of isolating and applying such probes. We have developed a simple technique that directs polymerase chain reaction (PCR) amplification to regions rich in variable number of tandem repeats (VNTRs). By using published VNTR core sequences as primers in PCRs, fragments were amplified that showed little variation within a species, but did show differences between species. When the amplified fragments were used as probes with genomic DNA Southern blots they produced hypervariable single-locus or few-locus patterns in fish, birds, and humans. We have named this procedure as Directed Amplification of Minisatellite-region DNA (DAMD).
Heath
D D
DD
Fisheries and Oceans Canada, West Vancouver Laboratory, BC.
Iwama
G K
GK
Devlin
R H
RH
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D003370:Coturnix; D004247:DNA; D017931:DNA Primers; D015342:DNA Probes; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D012474:Salmon; D013045:Species Specificity
D017931:DNA Primers; D015342:DNA Probes; D004247:DNA