Impact of organosulfur compounds in garlic on canine mammary tumor cells in culture.
Six organosulfur compounds found in garlic were examined for their ability to alter the growth of canine mammary tumor cells (CMT-13) in culture. Water-soluble organosulfur compounds (S-allyl-cysteine, S-ethyl-cysteine and S-propyl-cysteine) did not significantly alter the growth of CMT-13 cells when added to cultures at 1.0 mM or less. However, oil-soluble organosulfur compounds (diallyl sulfide, diallyl disulfide and diallyl trisulfide) markedly inhibited growth. Increasing addition of diallyl disulfide (DADS) resulted in a progressive decrease in CMT-13 cell growth. Addition of glutathione before DADS markedly decreased the severity of the growth inhibition. Treatment with DL-buthionine-SR-sulfoxamine, a specific inhibitor of glutathione synthesis, accentuated the growth inhibition caused by DADS. These studies show that some organosulfur compounds found in garlic are effective inhibitors of the growth of the neoplastic CMT-13 cell. The inhibitory effects of these compounds are modified by intracellular glutathione.
Sundaram
S G
SG
Department of Nutrition, Pennsylvania State University, University Park 16802.
Milner
J A
JA
D000498:Allyl Compounds / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004220:Disulfides / Q000494:pharmacology; D004285:Dogs; D005260:Female; D005737:Garlic; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology*; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy / Q000503:physiopathology*; D010938:Plant Oils / Q000494:pharmacology; D010946:Plants, Medicinal; D013440:Sulfides / Q000494:pharmacology; D013455:Sulfur / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D004220:Disulfides; D006131:Growth Inhibitors; D010938:Plant Oils; D013440:Sulfides; C042577:diallyl trisulfide; C028009:diallyl disulfide; C038491:allyl sulfide; C028742:S-propylcysteine; D013455:Sulfur; C065299:S-allylcysteine; C026826:S-ethylcysteine; D005978:Glutathione; D003545:Cysteine