title: A microtitre plate method for measuring biopterin with cryopreserved Crithidia fasciculata.
abstract: The assay of biopterin derivatives in dried blood spots is used by us in initial screening for inherited defects in tetrahydrobiopterin synthesis. The previously described method (1) required aseptic technique and microbiological facilities. The modification detailed here has the advantages of antibiotic cover, which overcomes these needs and microtitre plate technology allowing the incubation time to be halved with precision and accuracy retained. Data reduction facilities may be applied.
authors.affiliation: Department of Clinical Chemistry, Childrens Hospital, Ladywood Middleway, Birmingham, United Kingdom.
authors.forename: R J
authors.initials: RJ
authors.lastname: Leeming
authors.forename: S K
authors.initials: SK
authors.lastname: Hall
authors.forename: H
authors.initials: H
authors.lastname: Friday
authors.forename: P
authors.initials: P
authors.lastname: Hurley
authors.forename: A
authors.initials: A
authors.lastname: Green
mesh_terms: D000328:Adult; D000592:Amino Acid Metabolism, Inborn Errors / Q000097:blood / Q000175:diagnosis*; D000818:Animals; D001708:Biopterins / Q000097:blood*; D016831:Crithidia fasciculata / Q000254:growth & development*; D015925:Cryopreservation; D006801:Humans; D007202:Indicators and Reagents; D010651:Phenylalanine Hydroxylase / Q000172:deficiency*; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods
chemical_list: D007202:Indicators and Reagents; D001708:Biopterins; D010651:Phenylalanine Hydroxylase