An ultrasensitive chemiluminoenzyme immunoassay for the quantification of human tissue kininogens: application to synovial membrane and cartilage.
A sandwich enzyme immunoassay using a chemiluminescent detection has been developed for the quantification of total human (high and low molecular weight) kininogens in tissue extracts. This assay uses monospecific polyclonal IgG labelled with alkaline phosphatase and the commercially available dioxetane derivatives as substrates, for the detection of immune complexes. This method exhibits a sensitivity level of 1 fmol/ml and allows a precise quantification of total kininogens in synovium and cartilage extracts. When characterized by Western blot, the immunoreactive material reveals the presence of both high and low molecular weight kininogens.
Legris
F
F
Faculté de Pharmacie, Université de Montréal, Quebec, Canada.
Martel-Pelletier
J
J
Pelletier
J P
JP
Colman
R
R
Adam
A
A
D000368:Aged; D002358:Cartilage, Articular / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007704:Kininogens / Q000032:analysis* / Q000276:immunology; D008163:Luminescent Measurements; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013583:Synovial Membrane / Q000737:chemistry*
D007704:Kininogens