title: [Analysis of peptide fragment insertions in the basic proteins of the bacteriophage M13, f1, and fd envelopes. Interconnection of structural characteristics of the envelope protein and viability of mutant phages].
abstract: An analysis of 12 peptide fragment insertions into the major coat protein (protein pVIII) of bacteriophages M13, f1 and fd has been done. To elucidate the relations between protein structural characteristics and viability of mutant phages, we used the program Pro-Anal. Correlations were found between phage viability and different physicochemical and structural characteristics of protein N-termini. Thus peptide insertions in nonviable phages have high indexes of alpha-helicity, volumes, and polarity as well as high moments (alpha-helical and beta-structural) of isoelectric point. On the other hand, high beta-turn indexes are correlated with viability. The most important factor which determines phage viability is the lack of positively charged amino acid residues on the C-terminal ends of peptide insertions. The correlations found hold for the pVIII proteins of four related phages-M13, IKe, If1 and I2-2. Based on these results, the rule of obtaining viable mutant phages with insertions in the major coat protein is suggested. A new site is described for peptide insertions--upstream of the first amino acid residue of the mature protein sequence.
authors.forename: A M
authors.initials: AM
authors.lastname: Eroshkina
authors.forename: O O
authors.initials: OO
authors.lastname: Minenkova
authors.forename: V I
authors.initials: VI
authors.lastname: Fomin
authors.forename: V A
authors.initials: VA
authors.lastname: Ivanisenko
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
mesh_terms: D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000737:chemistry* / Q000235:genetics; D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics; D002213:Capsid / Q000737:chemistry*; D008969:Molecular Sequence Data; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D010446:Peptide Fragments
vernacular_title: Analiz vstroek peptidnykh fragmentov v osnovnoĭ belok obolochki bakteriofagov M13, f1, i fd. Vzaimosviaz' strukturnykh kharakteristik belka obolochki i zhiznesposobnosti mutantnykh fagov.