Characterization of different electrophoretic forms of cauliflower mosaic virus virions (strain Cabb-S).
The electrophoretic forms of purified cauliflower mosaic virus (CaMV), strain Cabb-S, were examined by electrophoresis on agarose gels. Three populations of viral particles were identified: a faster migrating component (the form F) and two slower migrating components (the forms S and S'). When the different forms of virions, after excision from gels, were subjected to analysis in SDS-polyacrylamide gel, the fast component consisted of the 37 and 42 kDa coat proteins whereas the slow components contained mainly the 39 kDa coat protein. However, there was no difference among the nucleic acids associated within the three forms. The biological significance of the different components is discussed.
Mesnard
J M
JM
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Mougeot
J L
JL
Geldreich
A
A
Lebeurier
G
G
D015139:Blotting, Southern; D002213:Capsid / Q000302:isolation & purification; D017796:Caulimovirus / Q000737:chemistry* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D014771:Virion / Q000737:chemistry* / Q000302:isolation & purification
D004279:DNA, Viral