An LC method for measuring complex formation equilibria by competitive chelation.
A method, entailing the use of a competitor ligand, is described for measuring complex formation constants by HPLC. The log of the formation constant of Gd(III) 2,6-bis(aminomethyl)pyridinetetraacetate was determined to be 18.6, a previously unreported value.
Chellquist
E M
EM
Pharmaceutical Sciences, Sterling Winthrop Pharmaceutical Research Division, Collegeville, PA 19426-0900.
Searle
R
R
D000085:Acetates / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003287:Contrast Media / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D008962:Models, Theoretical; D011725:Pyridines / Q000737:chemistry*
D000085:Acetates; D002614:Chelating Agents; D003287:Contrast Media; D011725:Pyridines; C085750:2,6-bis(aminomethyl)pyridinetetraacetate; D005682:Gadolinium