Vanadium induced hemolysis of vitamin E deficient erythrocytes in Hepes buffer.
Several vanadium compounds were tested for their ability to induce in vitro hemolysis of vitamin E-deficient hamster erythrocytes. Free vanadyl caused hemolysis in Hepes buffer but not in Tris or phosphate buffer, while hemolysis was inhibited by catalase, chelators such as deferoxamine mesylate and EDTA, and hydroxyl radical scavengers such as ethanol and D-mannitol. Although metavanadate itself could not induce hemolysis, metavanadate with NAD(P)H caused hemolysis in Hepes buffer only, and superoxide dismutase prevented it. Hydrogen peroxide, hydroxyl radical and Hepes radical were involved in vanadyl-induced hemolysis, superoxide anion was further involved in metavanadate plus NAD(P)H-induced hemolysis. Vitamin E prevented hemolysis under both conditions.
Hamada
T
T
Laboratory of Trace Elements Nutrition, National Institute of Animal Industry, Ibaraki, Japan.
D000704:Analysis of Variance; D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology; D006224:Cricetinae; D003676:Deferoxamine / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D016166:Free Radical Scavengers; D005609:Free Radicals / Q000378:metabolism; D006531:HEPES; D006461:Hemolysis; D017665:Hydroxyl Radical / Q000633:toxicity*; D008297:Male; D008647:Mesocricetus; D009249:NADP / Q000378:metabolism; D010710:Phosphates; D014325:Tromethamine; D014638:Vanadates / Q000633:toxicity; D014639:Vanadium / Q000633:toxicity*; D014811:Vitamin E Deficiency / Q000097:blood* / Q000175:diagnosis
D002614:Chelating Agents; D016166:Free Radical Scavengers; D005609:Free Radicals; D010710:Phosphates; D014639:Vanadium; D014325:Tromethamine; D017665:Hydroxyl Radical; D014638:Vanadates; D009249:NADP; D003676:Deferoxamine; D006531:HEPES