Identification of bovine B cell reactive and B cell specific monoclonal antibodies.
All monoclonal antibodies (mAbs) submitted to the workshop panel were screened for reactivity with bovine surface immunoglobulin (sIg)+ cells (gated small dense lymphocytes from peripheral blood) by fluorescence activated cell sorter (FACS) analysis. Eighteen temporary clusters--TCs 1-12, 15, 16, 18, 19, 25 and 26--contained mAbs reactive with sIg+ cells. mAb BAS21A (unclustered) and CC92 (TC25) were also reactive with sIg+ cells. Further FACS analysis with B cells from peripheral blood lymphocytes and mesenteric lymph nodes, and B and T lymphoma cell lines, indicated that the majority of mAbs within TCs 2, 4, 15, 18 and 26 reacted specifically with bovine B cells. Bovine B cell specific mAbs within these clusters were TH14B, IL-A55, CACT101A, MUC76A from TC4, VPM30, GC65A, CACT65A from TC15, IL-A58, CC56, CC70, IL-A65 from TC18, and CC57 and 26A9 from TC26. Three mAbs--IL-A65, CC70, and BAQ15A--within TC18 defined WC3; mAbs TD9 and CC56 may also be related to WC3.
Mukwedeya
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Institute for Animal Health, Pirbright Laboratory, Woking, UK.
Takamatsu
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Parkhouse
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008198:Lymph Nodes / Q000276:immunology; D011233:Precipitin Tests / Q000662:veterinary; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D011947:Receptors, Antigen, B-Cell