[Effects of Ganoderma polysaccharides on the activity of DNA polymerase alpha of splenocytes and immune function in aged mice].
The activity of DNA polymerase alpha in splenocytes of 24-month-old mice was about 35.6% lower than that of 3-month-old mice. Aged mice were intraperitoneally administered Ganoderma polysaccharides (GL-B) once a day for 4 days and then the activity of the enzyme was assessed. The results showed that GL-B at doses of 25 and 50 mg/kg-1 enhanced the activity of the enzyme in aged mouse splenocytes by 44.0 and 58.8% respectively. In addition, the mixed lymphocyte response to alloantigen, automatic proliferation and IL-2 production of splenocytes in aged mice declined as compared with that in young adult mice. GL-B (50, 100, 200 micrograms.ml-1) was found to restore those parameters to the levels of that of young mice in vitro.
Lei
L S
LS
Department of Pharmacology, Beijing Medical University, China.
Lin
Z B
ZB
D000375:Aging / Q000187:drug effects; D000818:Animals; D004257:DNA Polymerase II / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011124:Polyporaceae / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology*; D013154:Spleen / Q000201:enzymology / Q000276:immunology*
D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D011134:Polysaccharides; D004257:DNA Polymerase II