Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus.
A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classified by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC 2.4.2.10) activity; the mutant strain belonging to the second group was deficient in orotidine-5'-monophosphate decarboxylase (OMPdecase; EC 4.1.1.23). These mutants are defective in the genes pyrF and pyrG respectively. The results from random spore analysis, tetrad analysis, and gene-centromere distances showed that these two markers are located in linkage group VI, with pyrG being a proximal marker and pyrF a distal one.
Campuzano
V
V
Departamento de Microbiología y Genética, Facultad de Biología, Universidad de Salamanca, Spain.
del Valle
P
P
de Vicente
J I
JI
Eslava
A P
AP
Alvarez
M I
MI
D002474:Cell-Free System; D004352:Drug Resistance, Microbial; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics*; D009963:Orotic Acid / Q000031:analogs & derivatives / Q000633:toxicity; D010800:Phycomyces / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism*; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013172:Spores, Fungal / Q000378:metabolism
D005819:Genetic Markers; D011743:Pyrimidines; D009963:Orotic Acid; C001242:5-fluoroorotic acid; D009962:Orotate Phosphoribosyltransferase