title: Expression of 11 beta-hydroxysteroid dehydrogenase mRNA in rat vascular smooth muscle cells.
abstract: The enzyme 11 beta-hydroxysteroid dehydrogenase (11-HSD) converts corticosterone to the inactive 11-dehydrocorticosterone in the rat. The bioactivity of 11-HSD, expressed as the percentage conversion of 3H-corticosterone to 3H-11-dehydrocorticosterone, was 13.7 +/- 0.4% in rat vascular smooth muscle cells (rVSMC). Cells treated with 100 nM dexamethasone (Dex) showed a 1.4-fold (p < 0.05) increase in 11-HSD activity. Insulin (100 microM) decreased 11-HSD activity by 0.8-fold (p < 0.05). Expression of 11-HSD mRNA was also confirmed in rVSMC by Northern blot analysis. Dexamethasone increased and insulin decreased the levels of 11-HSD mRNA in parallel with the increase in bioactivity. Vascular smooth muscle cells express 11-HSD activity; the access of corticosterone to vascular smooth muscle receptors may be modulated by the enzyme.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takeda
authors.forename: I
authors.initials: I
authors.lastname: Miyamori
authors.forename: T
authors.initials: T
authors.lastname: Yoneda
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
authors.forename: R
authors.initials: R
authors.lastname: Takeda
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases; D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000031:analogs & derivatives / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics*; D006913:Hydroxysteroid Dehydrogenases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D013268:Stimulation, Chemical; D014316:Tritium
chemical_list: D007328:Insulin; D012333:RNA, Messenger; D014316:Tritium; D003907:Dexamethasone; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; C003552:11-dehydrocorticosterone; D003345:Corticosterone