Ribosome-messenger recognition in the absence of the Shine-Dalgarno interactions.
In an attempt to understand how Escherichia coli ribosomes recognize the initiator codon on mRNAs lacking the Shine-Dalgarno (SD) sequence, we have studied 30S initiation complex formation in extension inhibition (toeprinting) experiments using (-SD)mRNAs which are known to be reliably translated in E. coli: the plant viral messenger A1MV RNA 4 and two chimaeric mRNAs coding for beta-glucuronidase (GUS) and bearing the 5'-untranslated sequence of TMV RNA (omega) or the omega-derived sequence (CAA)n as 5'-leaders. Ribosomal protein S1 and IF3 have been found to be indispensable for translational initiation. Protein S1 appears to be a key recognition element. S1 binds to sequences within the leaders of (-SD)mRNAs thus providing their affinity to E. coli ribosomes.
Tzareva
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M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Makhno
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Boni
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D017794:Alfalfa mosaic virus / Q000235:genetics; D001483:Base Sequence; D003062:Codon / Q000378:metabolism; D017931:DNA Primers; D004926:Escherichia coli / Q000378:metabolism*; D005966:Glucuronidase / Q000096:biosynthesis; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D014027:Tobacco Mosaic Virus / Q000235:genetics
D003062:Codon; D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral; D012269:Ribosomal Proteins; D005966:Glucuronidase