Comparison of the intracellular pathways of immunoglobulin-G and low density lipoprotein in cultured human term trophoblast cells.
Trophoblast cells were cultured on microporous membrane filters. After incubation at different times with gold-conjugated ligands, the cells were processed for electron microscopy. Gold particles indicating the presence of both IgG and LDL appeared in a time-dependent manner in coated pits and coated vesicles. LDL-gold appeared primarily within lysosomes whereas approximately 50% of the internalized IgG-gold appeared within vesicles (diameters ranging from 35 to 80 nm) near the basal regions of the cell. These vesicles may be the protective mechanism which prevents IgG breakdown during transcytosis across trophoblast cells, thus allowing transport of the intact molecule to the fetus.
Sooranna
S R
SR
Academic Department of Obstetrics and Gynaecology, Chelsea and Westminster Hospital, London, UK.
Moss
J
J
Contractor
S F
SF
D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008077:Lipoproteins, LDL / Q000276:immunology / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000648:ultrastructure; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D014327:Trophoblasts / Q000378:metabolism* / Q000648:ultrastructure
D007074:Immunoglobulin G; D008077:Lipoproteins, LDL