Production and in vivo use of recombinant ovine IL-1 beta as an immunological adjuvant.
To determine the potential of ovine interleukin 1 (IL-1) as a vaccine adjuvant in sheep, we have expressed and purified recombinant ovine IL-1 beta (rovIL-1 beta) from bacterial cultures using a modified form of the ovine IL-1 beta cDNA. Adjuvant trials using the model protein avidin demonstrated that rovIL-1 beta when administered in association with a compound providing a slow-release mechanism, resulted in significant enhancement of specific serum antibody levels in both mice and sheep. In a dose-response experiment in sheep, intradermal immunization with avidin plus either 10 or 100 micrograms of rovIL-1 beta in aluminium hydroxide resulted in antibody levels four- to eightfold higher than immunizations without rovIL-1 beta. The addition of rovIL-1 beta also resulted in a more severe DTH response to avidin indicating that rovIL-1 beta is able to enhance both humoral and cell-mediated responses to avidin. The highest antibody titres were observed when sheep received rovIL-1 beta in both the primary and secondary immunizations although the addition of rovIL-1 beta in only one of the immunizations still resulted in a significant increase in antibody levels. Additional experiments showed that rovIL-1 beta and avidin must be administered in a site drained by the same lymph node for the adjuvant effect of rovIL-1 beta to be observed.
Andrews
A E
AE
Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Lofthouse
S A
SA
Bowles
V M
VM
Brandon
M R
MR
Nash
A D
AD
D000276:Adjuvants, Immunologic / Q000096:biosynthesis* / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001360:Avidin / Q000276:immunology*; D001483:Base Sequence; D002478:Cells, Cultured; D005260:Female; D006968:Hypersensitivity, Delayed; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology* / Q000494:pharmacology; D012756:Sheep
D000276:Adjuvants, Immunologic; D007375:Interleukin-1; D011994:Recombinant Proteins; D001360:Avidin