Immortalized GABAergic cell lines derived from rat striatum using a temperature-sensitive allele of the SV40 large T antigen.
Embryonic striatal cells were immortalized using the A58 temperature-sensitive allele of the SV40 large T antigen. Two cell lines, M213-2O and M26-1F, with gamma-aminobutyric acid (GABA)ergic properties were selected from 85 clones thus developed. M213-2O is a multipolar, polygonal cell line which expresses SV40 large T antigen and glutamate decarboxylase (GAD) at the permissive temperature (33 degrees C) and GAD and MAP-2 immunoreactivity at the nonpermissive temperature (39.3 degrees C). M26-1F has a fibroblast-like morphology and expresses SV40 large T antigen and GAD both at the permissive and nonpermissive temperatures and MAP-2 immunoreactivity at the nonpermissive temperature. Both lines contain GABA as measured by reversed-phase HPLC and M213-2O expresses nipecotic-sensitive [14C]GABA uptake.
Giordano
M
M
NIMH-Neuroscience Center, St. Elizabeths, Washington, DC 20032.
Takashima
H
H
Herranz
A
A
Poltorak
M
M
Geller
H M
HM
Marone
M
M
Freed
W J
WJ
D000483:Alleles; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis*; D001692:Biological Transport; D015415:Biomarkers; D002461:Cell Line, Transformed; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D005333:Fetus; D005968:Glutamate Decarboxylase / Q000096:biosynthesis*; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013539:Simian virus 40 / Q000235:genetics*; D013696:Temperature; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000952:Antigens, Polyomavirus Transforming; D015415:Biomarkers; D008869:Microtubule-Associated Proteins; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase