title: Purification and characterisation of the restriction endonuclease ItaI from Ilyobacter tartaricus recognizing 5'-GC decreases NGC-3'.
abstract: ItaI, an isoschizomer of the subclass IIW [Kessler and Manta, Gene 92 (1990) 1-248] restriction endonuclease (ENase), Fnu4HI [Leung et al., Nucleic Acids Res. 6 (1979) 17-25], has been isolated from Ilyobacter tartaricus. The ENase has the five-base palindromic recognition sequence, 5'GC decreases NGC-3'. It cleaves behind the second nucleotide and produces a one-nt 5' overhang.
authors.affiliation: Department of Microbiology, Ruhr-Universität Bochum, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Städtler
authors.forename: R P
authors.initials: RP
authors.lastname: von Strandmann
authors.forename: T
authors.initials: T
authors.lastname: Walter
authors.forename: B
authors.initials: B
authors.lastname: Frey
authors.forename: H
authors.initials: H
authors.lastname: Auer
authors.forename: W
authors.initials: W
authors.lastname: Hengstenberg
authors.forename: G
authors.initials: G
authors.lastname: Schmitz
mesh_terms: D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology*; D012091:Repetitive Sequences, Nucleic Acid; D013379:Substrate Specificity
chemical_list: C069758:endodeoxyribonuclease Fnu4HI; D015252:Deoxyribonucleases, Type II Site-Specific