Molecular cloning of the CD45-associated 30-kDa protein.
CD45, a leukocyte-specific transmembrane protein tyrosine phosphatase, mediates signal transduction pathways critical for immune responses. However, the mechanism of CD45-mediated signal transduction and the identity of CD45-associated proteins have remained unclear. A CD45-associated 30-kDA phosphorylated protein (CD45-AP) was purified by virtue of its specific association with CD45, and its mouse cDNA was cloned by using the internal amino acid sequence information. In vitro translated CD45-AP bound specifically to CD45. CD45-AP appears to be leukocyte-specific and shares no significant homology with presently known sequences. The predicted sequence contains no consensus tyrosine phosphorylation sites or conserved sequences of GTP-binding proteins. CD45-AP may act as an adapter molecule for CD45-mediated signal transduction.
Takeda
A
A
Department of Pathology, Roger Williams Medical Center, Brown University, Providence, Rhode Island 02908.
Maizel
A L
AL
Kitamura
K
K
Ohta
T
T
Kimura
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002846:Chromatography, Affinity; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000096:biosynthesis / Q000378:metabolism; D015723:Gene Library; D047908:Intracellular Signaling Peptides and Proteins; D007700:Kinetics; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D013601:T-Lymphocytes
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; C513303:Ptprcap protein, mouse; D012333:RNA, Messenger; D011994:Recombinant Proteins; D017493:Leukocyte Common Antigens