Isolation of CD3-, CD4-, CD8-, IL-2R+ thymocyte precursors by panning.
We present a panning method for isolation of thymocytes that are CD3-, CD4-, CD8- and IL-2R+. These cells have been isolated by positive selection on dishes coated with 7D4 antibody followed by treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.
Small
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Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.
Majumdar
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Lieberman
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Weissman
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D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology*
D015703:Antigens, CD; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D015375:Receptors, Interleukin-2