Immunofluorescent diagnosis of Chlamydia trachomatis infection of the respiratory tract and the eye.
Direct and indirect immunofluorescent antibodies as well as IgM antichlamydia antibodies were studied for diagnosis of 60 patients with Chlamydia trachomatis infection as well as 20 normal controls (group C). 30 Patients were suffering from C. trachomatis pneumonia (group A) and the other 30 patients were suffering from trachoma eye infection (group B). Indirect immunofluorescent technique (IIF) was positive 9.9% of group A. IgM antichlamydia antibodies by ELISA was positive in 23.3% and 16.6% in group "A" and "B" respectively. While highly positive results (60%) were obtained in conjunctival smears of group "B" when using direct immunofluorescent staining.
Abdel Rahman
M M
MM
Department of Tropical Medicine, Faculty of Medicine, Al-Azhar University, Cairo.
Abdel Dayem
S I
SI
Eid
S A
SA
Badie
O A
OA
Kotb
N A
NA
D000907:Antibodies, Bacterial / Q000097:blood; D002648:Child; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D011014:Pneumonia / Q000175:diagnosis*; D014141:Trachoma / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M