Oxidative susceptibility of low density lipoprotein subfractions is related to their ubiquinol-10 and alpha-tocopherol content.
The conjugated polyene fatty acid parinaric acid (PnA) undergoes a stoichiometric loss in fluorescence upon oxidation and can be used to directly monitor peroxidative stress within lipid environments. We evaluated the course of potentially atherogenic oxidative changes in low density lipoproteins (LDL) by monitoring the oxidation of PnA following its incorporation into buoyant (p = 1.026-1.032 g/ml) and dense (p = 1.040-1.054 g/ml) LDL subfractions. Copper-induced oxidation of LDL-associated PnA exhibited an initial lag phase followed by an increased rate of loss until depletion. Increased PnA oxidation occurred immediately after the antioxidants ubiquinol-10 and alpha-tocopherol were consumed but before there were marked elevations in conjugated dienes. Despite differences in sensitivity to early oxidation events, PnA oxidation and conjugated diene lag times were correlated (r = 0.582; P = 0.03), and both indicated a greater susceptibility of dense than buoyant LDL in accordance with previous reports. The greater susceptibility of PnA in dense LDL was attributed to reduced levels of ubiquinol-10 and alpha-tocopherol, which were approximately 50% lower than in buoyant LDL (mol of antioxidant/mol of LDL) and together accounted for 80% of the variation in PnA oxidation lag times. These results suggest that PnA is a useful probe of LDL oxidative susceptibility and may be superior to conjugated dienes for monitoring the initial stages of LDL lipid peroxidation. Differences in oxidative susceptibility among LDL density subfractions are detected by the PnA assay and are due in large part to differences in their antioxidant content.
Tribble
D L
DL
Department of Molecular and Nuclear Medicine, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
van den Berg
J J
JJ
Motchnik
P A
PA
Ames
B N
BN
Lewis
D M
DM
Chait
A
A
Krauss
R M
RM
D000328:Adult; D000975:Antioxidants / Q000032:analysis; D001161:Arteriosclerosis / Q000209:etiology; D005231:Fatty Acids, Unsaturated; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D008297:Male; D010084:Oxidation-Reduction; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis; D014810:Vitamin E / Q000032:analysis*
D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D008077:Lipoproteins, LDL; D014451:Ubiquinone; D014810:Vitamin E; C026663:ubiquinol-10; C013641:parinaric acid