title: Precipitation with KCl improves the chemical cross-linking of GST-fusion proteins to agarose after solubilization with SDS.
authors.affiliation: College of Pharmacy, University of Arizona, Tucson.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: P
authors.initials: P
authors.lastname: Vanscheeuwijck
authors.forename: J W
authors.initials: JW
authors.lastname: Regan
mesh_terms: D011232:Chemical Precipitation; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005941:Glucokinase / Q000737:chemistry / Q000235:genetics; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011189:Potassium Chloride; D011942:Receptors, Adrenergic, alpha / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D012685:Sepharose / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
chemical_list: D003432:Cross-Linking Reagents; D011942:Receptors, Adrenergic, alpha; D011993:Recombinant Fusion Proteins; D012967:Sodium Dodecyl Sulfate; D011189:Potassium Chloride; D012685:Sepharose; D005982:Glutathione Transferase; D005941:Glucokinase