title: Identification of mycotoxins in keratomycosis-derived Fusarium isolates by gas chromatography-mass spectrometry.
abstract: The production of mycotoxins from Fusarium species has been demonstrated in isolates cultured from patients suffering from keratomycosis. The method employed a combination of thin-layer chromatography directly performed on gel plugs taken from the growth medium, cartridge column chromatography, silylation and gas chromatography on a non-polar stationary phase capillary column linked to mass spectrometry. The sensitivities of detection obtained for a signal-to-noise ratio of 33:1, were 200 pg for single stage GC-MS and 20 pg using tandem GC-MS-MS. Two mycotoxins, diacetoxyscirpenol and T-2 toxin were identified in three cultures.
authors.affiliation: St John's Institute of Dermatology, UMDS, University of London, St. Thomas' Hospital, UK.
authors.forename: S K
authors.initials: SK
authors.lastname: Raza
authors.forename: S A
authors.initials: SA
authors.lastname: Howell
authors.forename: A I
authors.initials: AI
authors.lastname: Mallet
mesh_terms: D002855:Chromatography, Thin Layer; D003316:Corneal Diseases / Q000382:microbiology*; D005670:Fusarium / Q000737:chemistry* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015394:Molecular Structure; D009181:Mycoses / Q000382:microbiology*; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry
chemical_list: D009183:Mycotoxins