Self-diploidization in Saccharomyces cerevisiae kar2 heterokaryons.
Zygotes isolated by micromanipulation from crosses of Saccharomyces cerevisiae strains, one of which carries a kar mutation, give rise most frequently to cytoductant colonies showing the nuclear constitution of either one of the two haploid parental strains. In crosses of kar2-1 strains to wild-type, about 10% of the cytoductants of both mating types are homozygous autodiploids. There is evidence indicating that self-diploidization occurs by fusion between sibling nuclei in the heterokaryotic zygote. Here we describe this phenomenon and propose to take advantage of it for the construction of genotypically-defined diploids able to mate, and of polyploid strains, which are useful tools in genetic and cytological studies.
Polaina
J
J
Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Valencia, Spain.
Adam
A C
AC
del Castillo
L
L
D003433:Crosses, Genetic; D004171:Diploidy; D005800:Genes, Fungal; D005838:Genotype; D007621:Karyotyping; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015053:Zygote