title: Expression of a synthetic DNA region containing a consensus promoter and two lac operators in Brevibacterium sp.
abstract: The cat reporter gene was used to assess expression of two promoters, previously strongly expressed in Escherichia coli, in Brevibacterium sp. R312 strain. The tac promoter (de Boer et al., 1983, Proc. Natl. Acad. Sci. USA 80, 21-25) was poorly expressed in Brevibacterium sp. In contrast, the AatII-SalI fragment of plasmid pYEJ001 (Pharmacia LKB Biotechnology, Sweden) containing two lac operators, a consensus sequence promoter and the cat structural gene clearly revealed chloramphenicol acetyltransferase activity and the presence of a 25,600-kDa protein, corresponding to the monomeric CAT protein, in cell extracts.
authors.affiliation: Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, ENSA, Montpellier, France.
authors.forename: C K
authors.initials: CK
authors.lastname: Chan Kwo Chion
authors.forename: A
authors.initials: A
authors.lastname: Arnaud
authors.forename: P
authors.initials: P
authors.lastname: Galzy
mesh_terms: D001483:Base Sequence; D001951:Brevibacterium / Q000235:genetics* / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000302:isolation & purification; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D007763:Lac Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase