Luminescence of Ca(2+)-activated photoprotein obelin initiated by NaOCl and MnCl2.
The luminescence of obelin is initiated by NaOCl in a reaction mixture containing no calcium. The addition of Mn2+ enhances the light emission > 300-fold. Sodium azide and histidine, as singlet oxygen quenchers, inhibit NaOCl-activated obelin luminescence in the presence or absence of Mn2+. This suggests that the addition of NaOCl to the mixture causes singlet oxygen formation (stimulated by Mn2+ ions), and singlet oxygen initiates the light-emitting reaction.
Vysotski
E S
ES
Laboratory of Photobiology, Russian Academy of Sciences, Krasnoyarsk.
Trofimov
K P
KP
Bondar'
V S
VS
Gitelson
J I
JI
D002118:Calcium / Q000378:metabolism*; D002712:Chlorides / Q000494:pharmacology*; D007700:Kinetics; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D017895:Manganese Compounds / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology*
D002712:Chlorides; D008164:Luminescent Proteins; D017895:Manganese Compounds; C010497:obelin; D012973:Sodium Hypochlorite; C025340:manganese chloride; D002118:Calcium