title: Effect of coffee drinking on cell proliferation in rat urinary bladder epithelium.
abstract: A possible effect of freshly brewed drip coffee on urinary bladder carcinogenesis was investigated in male Wistar rats using cell proliferation in urinary bladder epithelium as the indicator of tumour promotion. Male rats were given either undiluted coffee brew (100% coffee), coffee diluted 10 times (10% coffee) or tap water (controls), as their only source of drinking fluid for 2 or 6 wk. Uracil, known to induce cell proliferation in urinary bladder epithelium, was included in the study as a positive control. In rats receiving 100% coffee, body weights, liquid intake and urinary volume were decreased. Neither histopathological examination of urinary bladder tissue nor the bromodeoxyuridine labelling index revealed biologically significant differences between rats receiving coffee and the tap water controls. Uracil increased the labelling index and induced hyperplasia of the urinary bladder epithelium, as expected. It was concluded that these results produced no evidence that drinking coffee predisposes to tumour development in the urinary bladder.
authors.affiliation: Department of Biological Toxicology, TNO-Toxicology and Nutrition Institute, Zeist, The Netherlands.
authors.forename: B A
authors.initials: BA
authors.lastname: Lina
authors.forename: A A
authors.initials: AA
authors.lastname: Rutten
authors.forename: R A
authors.initials: RA
authors.lastname: Woutersen
mesh_terms: D000818:Animals; D001835:Body Weight; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D003069:Coffee / Q000633:toxicity*; D004326:Drinking; D004573:Electrolytes / Q000652:urine; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D006863:Hydrogen-Ion Concentration; D006965:Hyperplasia; D008297:Male; D011897:Random Allocation; D051381:Rats; D014498:Uracil / Q000633:toxicity; D001743:Urinary Bladder / Q000187:drug effects / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000209:etiology*; D014556:Urine / Q000737:chemistry
chemical_list: D003069:Coffee; D004573:Electrolytes; D014498:Uracil