Immunoelectron microscopic localization of lactase-phlorizin hydrolase in rat small intestine.
To provide insight into the intracellular translocation of lactase-phlorizin hydrolase, an immunoelectron microscopy was performed on rapidly embedded Lowicryl K4M sections of rat jejunum. Lactase-phlorizin hydrolase immunoreactivity was detected not only in the microvillous membranes and in the smooth apical vesicles, but also in the lateral membranes, suggesting an alternative route for intracellular transport of lactase-phlorizin hydrolase via the lateral membranes to the microvilli.
Noda
S
S
Department of Morphology, School of Medicine, Tokai University, Isehara, Japan.
Goda
T
T
D000818:Animals; D000833:Animals, Suckling; D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology* / Q000648:ultrastructure; D007583:Jejunum / Q000201:enzymology / Q000648:ultrastructure; D043322:Lactase; D010694:Lactase-Phlorizin Hydrolase / Q000032:analysis*; D016253:Microscopy, Immunoelectron; D008871:Microvilli / Q000201:enzymology; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley; D014886:Weaning; D001616:beta-Galactosidase / Q000032:analysis*
D011498:Protein Precursors; D043322:Lactase; D001616:beta-Galactosidase; D010694:Lactase-Phlorizin Hydrolase