Induction of glandular stomach cancers in C3H mice treated with N-methyl-N-nitrosourea in the drinking water.
Establishment of an animal model of stomach carcinogenesis in mice was attempted using N-methyl-N-nitrosourea (MNU) in the drinking water. One hundred and forty-eight male 6-week-old C3H mice were given MNU in their drinking water at a concentration of 120 ppm (group 1), 60 ppm (group 2), 30 ppm (group 3) or 0 ppm (group 4) for 30 weeks. At the end of this time, dose-related induction of adenomatous hyperplasias was found. From weeks 31 to 54 adenocarcinomas developed in a dose-dependent manner in groups 1, 2 and 3. In total, 6 well differentiated and 5 poorly differentiated adenocarcinomas as well as 6 signet ring cell carcinomas arose in 15 stomach cancer-bearing animals in group 1, 4 well differentiated and 2 poorly differentiated adenocarcinomas with one signet ring cell carcinoma in 5 mice of group 2 and one well differentiated adenocarcinoma in group 3. In the forestomach, only one squamous cell carcinoma was found at week 54 in group 1 along with a single well differentiated adenocarcinoma in the duodenum. Thus, MNU in the drinking water selectively induced neoplastic lesions in the glandular stomach epithelium of mice.
Tatematsu
M
M
Laboratory of Pathology, Aichi Cancer Center Research Institute, Nagoya.
Yamamoto
M
M
Iwata
H
H
Fukami
H
H
Yuasa
H
H
Tezuka
N
N
Masui
T
T
Nakanishi
H
H
D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology; D000818:Animals; D018279:Carcinoma, Signet Ring Cell / Q000139:chemically induced / Q000473:pathology; D004032:Diet; D004195:Disease Models, Animal; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D008297:Male; D008770:Methylnitrosourea / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D008809:Mice, Inbred C3H; D013274:Stomach Neoplasms / Q000139:chemically induced* / Q000473:pathology
D008770:Methylnitrosourea