Histatin as a synergistic stimulator with epidermal growth factor of rabbit chondrocyte proliferation.
Histatin 5 dose-dependently increased DNA synthesis in rabbit costal chondrocytes in culture. The level of DNA synthesis was stimulated by histatin 5 to about 4 times that of the control. The combination of histatin 5 and epidermal growth factor (EGF) increased the DNA synthesis to about 40 times that of the control while EGF alone stimulated it 15-fold, indicating synergistic stimulation by both factors. Enzyme-linked immunosorbent assay using anti-histatin 5 demonstrated that human serum contained histatin-like substances. These findings suggest that histatins play an important role in chondrocyte proliferation, presumably as a physiological modulator of the action of EGF.
Murakami
Y
Y
Department of Preventive Dentistry, Osaka University Faculty of Dentistry, Japan.
Nagata
H
H
Shizukuishi
S
S
Nakashima
K
K
Okawa
T
T
Takigawa
M
M
Tsunemitsu
A
A
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis*; D004357:Drug Synergism; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D054808:Histatins; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology; D010306:Parotid Gland / Q000502:physiology; D011817:Rabbits; D012272:Ribs; D012463:Saliva / Q000502:physiology; D012471:Salivary Proteins and Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
C068075:HTN3 protein, human; D054808:Histatins; D009842:Oligopeptides; D012471:Salivary Proteins and Peptides; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D004247:DNA; D013936:Thymidine