title: Determination of cyclodextrins in serum by reversed-phase chromatography with pulsed amperometric detection and a membrane reactor.
abstract: A high-performance liquid chromatographic method has been developed for the determination of cyclodextrins (CDs) in serum. The method involves solid-phase extraction of CDs, separation on a C18 reversed-phase column using a mixture of water, tetrahydrofuran and methanol as an eluent, eluent pH modification with a cation-exchange membrane reactor surrounded by 1.5 M sodium hydroxide solutions, and pulsed amperometric detection (PAD) with a gold working electrode. The solid-phase extraction on a C18 bonded-silica column was effective for removing the PAD sensitive components in serum. The calibration graphs constructed by internal standard method were linear over the range 6.25-200 pmol of CDs in serum. The detection limits for CDs were about 5 pmol at a signal-to-noise ratio of 3.
authors.affiliation: Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Haginaka
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimura
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003505:Cyclodextrins / Q000097:blood*; D004563:Electrochemistry; D006801:Humans
chemical_list: D003505:Cyclodextrins