Ligation of N-acetylgalactosamine-containing structures on rat bone marrow cells enhances myeloid differentiation and murine granulocyte-macrophage colony-stimulating factor-induced proliferation.
Previously we have reported the differentiation-dependent expression of a soybean agglutinin (SBA)-binding structure on rat bone marrow cells (BMCs) during their differentiation into macrophages (m phi s). In the present study we tried to analyze the functional role of the SBA-binding structure in BMC proliferation and differentiation. Addition of SBA to BMC cultures driven into m phi differentiation by recombinant murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF), resulted in a two- to threefold increased proliferation rate compared with rmGM-CSF alone. However, the number of colonies in methyl cellulose was not increased by SBA. The effect of SBA was dose dependent (from 4 to 83 pM SBA), with a maximum effect at 83 pM. Experiments to detect a possible synergistic effect of additional cytokines produced by BMC after SBA treatment were inconclusive. The enhancing effect of SBA was also seen when high-density cells, which did not proliferate in response to rmGM-CSF (mainly granulocytes), were removed. Therefore, SBA may increase the CSF reactivity of responsive m phi progenitor cells directly by binding to N-acetylgalactosamine residues on their surface.
Krugluger
W
W
Institute of General and Experimental Pathology, University of Vienna, Austria.
Köller
M
M
Allmaier
M
M
Boltz-Nitulescu
G
G
Förster
O
O
D000116:Acetylgalactosamine / Q000502:physiology*; D000818:Animals; D001854:Bone Marrow Cells; D002452:Cell Count; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology*; D006410:Hematopoiesis; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D037121:Plant Lectins; D051381:Rats; D011917:Rats, Inbred Lew; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D000116:Acetylgalactosamine; D004247:DNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007377:Interleukin-3; D015850:Interleukin-6; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins