title: Cis-hydroxyproline inhibits adhesion, migration and proliferation of cultured rabbit keratocytes.
abstract: Recruitment of keratocytes into injured corneal stroma, and secretion of proteins including collagen in the cells are essential for wound healing of the corneal stroma. We examined the effect of a proline analog, cis-hydroxyproline, on the adhesion, migration and growth of rabbit keratocytes in vitro. This agent decreased the plating efficiency, migration and growth of the keratocytes in a dose-dependent manner. Reduction in these cellular activities may reflect altered functions of pericellular proteins such as collagen. Further studies are needed to determine which specific protein is involved.
authors.affiliation: Department of Ophthalmology, Wakayama Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saika
authors.forename: O
authors.initials: O
authors.lastname: Yamanaka
authors.forename: N
authors.initials: N
authors.lastname: Hashizume
authors.forename: S
authors.initials: S
authors.lastname: Kobata
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: K
authors.initials: K
authors.lastname: Uenoyama
authors.forename: A
authors.initials: A
authors.lastname: Ooshima
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D003319:Corneal Stroma / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006909:Hydroxyproline / Q000494:pharmacology*; D011817:Rabbits; D014945:Wound Healing
chemical_list: D003094:Collagen; D004247:DNA; D006909:Hydroxyproline