Antimetabolite-induced increases in the invasive capacity of murine leukaemia L1210 cells.
Pretreatment of murine leukaemia L1210 cells with non-lethal concentrations of various antimetabolites increased the in vitro invasive capacity of these cells into monolayers of rat embryo fibroblasts. The increase in invasive capacity was partly correlated with the induced cell cycle arrest. The concomitant increase in cell surface fucosylation and inhibition of invasion with sulphate indicate a role for glycoproteins in this process. Our results suggest that treatment with antimetabolites may lead to a more aggressive phenotype by altering cell surface properties.
De Graaf
T W
TW
Department of Medical Chemistry, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
Peters
G J
GJ
van Dijk
W
W
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D002453:Cell Cycle; D002455:Cell Division; D005347:Fibroblasts; D005434:Flow Cytometry; D006031:Glycosylation / Q000187:drug effects; D007939:Leukemia L1210 / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D051381:Rats; D014407:Tumor Cells, Cultured
D000963:Antimetabolites