title: Improved methods for the cultivation of strictly anaerobic, extremely thermophilic methanogens.
abstract: The strictly anaerobic, extremely thermophilic methanogens, Methanobacterium thermoautotrophicum Marburg and M. thermoautotrophicum delta H, have been cultivated in liquid culture and on solid medium in screw-top bottles, which permit continuous monitoring of the growth of the microorganisms. We have been able to routinely grow methanogens in medium containing bicarbonate, TRIS or 4-morpholinepropanesulfonic acid (MOPS) buffers and three different sulfur sources (sulfide, sulfite and thiosulfate) at temperatures up to 70 degrees C and at pressures up to 35 psi while monitoring cell density or colony formation.
authors.affiliation: Southern Illinois University at Carbondale.
authors.forename: M S
authors.initials: MS
authors.lastname: Foster
authors.forename: A
authors.initials: A
authors.lastname: Rodabough
authors.forename: T M
authors.initials: TM
authors.lastname: Dayton
authors.forename: E M
authors.initials: EM
authors.lastname: Melko
authors.forename: S S
authors.initials: SS
authors.lastname: Szegedi
authors.forename: E C
authors.initials: EC
authors.lastname: Niederhoffer
mesh_terms: D000693:Anaerobiosis; D001431:Bacteriological Techniques; D001639:Bicarbonates; D002021:Buffers; D002452:Cell Count; D003470:Culture Media; D017014:Methanobacterium / Q000254:growth & development*; D009025:Morpholines; D011312:Pressure; D013696:Temperature; D014325:Tromethamine
chemical_list: D001639:Bicarbonates; D002021:Buffers; D003470:Culture Media; D009025:Morpholines; D014325:Tromethamine; C008550:morpholinopropane sulfonic acid