Liquid chromatography and radioimmunoassay method for the determination of prostaglandins E1 and E2 in rat embryo incubates.
This paper describes the application of a combined high-performance liquid chromatography and radioimmunological assay method for the measurement of prostaglandins E1(PGE1) and E2(PGE2). Samples were acidified to pH 3.15, extracted twice with ethyl acetate and further processed through C18 solid-phase extraction cartridges. After HPLC purification, PGE1 and PGE2 were measured by radioimmunological techniques. The limit of detection for PGE1 was 3.9 pg/ml and the intra-assay relative standard deviation was 7.8% for n = 5. The accuracy of the assay procedure was also verified. The method has been applied to the determination of PGE1 and PGE2 in embryo incubates from 10-day pregnant rats.
Hotter
G
G
Molecular Pathology Unit, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
Roselló-Catafau
J
J
Closa
D
D
Bioque
G
G
Gelpí
E
E
Javerbaum
A
A
González
E
E
Gimeno
M A
MA
D000527:Alprostadil / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone / Q000032:analysis*; D004622:Embryo, Mammalian / Q000737:chemistry*; D011863:Radioimmunoassay; D051381:Rats
D000527:Alprostadil; D015232:Dinoprostone