Effect of sepsis-related cytokines on lipid synthesis by isolated human hepatocytes.
OBJECTIVE
To investigate the effect of tumour necrosis factor-alpha (TNF-alpha) and other sepsis related cytokines on the synthesis of several lipid fractions by human hepatocytes.


METHODS
Laboratory study.


METHODS
University departments of surgery and biochemistry.


METHODS
Livers from eight donors of other organs.


METHODS
Effect of TNF-alpha, interleukin-1 (IL-1), and interleukin-6 (IL-6) on the synthesis of triacylglycerol and phospholipids by freshly isolated human hepatocytes.


RESULTS
All three cytokines, particularly TNF-alpha, inhibited the incorporation of D-glucose uniformly labelled with 14C (D-(U-14C) glucose) into phosphatidylcholine. The incorporation of D-(U-14C) glucose into triacylglycerol and free fatty acids was significantly stimulated by TNF-alpha and IL-1, but not by IL-6. When palmitate uniformly labelled with 14C was used as the radiolabelled precursor, TNF-alpha stimulated the synthesis of both triacylglycerol and phosphatidylcholine, neither of which was affected by IL-1 or IL-6.


CONCLUSIONS
Changes that are induced by cytokines in lipid synthesis by hepatocytes can contribute to the impairment of lipid metabolism seen in septic patients. TNF-alpha seemed to have more effect than the other two cytokines.
Arias-Díaz
J
J
Department of Surgery, U. Complutense de Madrid, Ciudad Universitaria, Spain.
Vara
E
E
Gómez
M
M
Moreno
A
A
Torres-Melero
J
J
Balibrea
J L
JL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology; D015850:Interleukin-6 / Q000502:physiology; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis* / Q000378:metabolism; D018805:Sepsis / Q000276:immunology; D014280:Triglycerides / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D010713:Phosphatidylcholines; D010743:Phospholipids; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha