title: Structural study of rat thyroid transcription factor 1 homeodomain (TTF-1 HD) by nuclear magnetic resonance.
abstract: The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously described for other homeodomains and expected also for TTF-1 HD from homology modeling predictions. The three-dimensional arrangement of the three helix fragments of TTF-1 HD preserves the helix-turn-helix motif commonly occurring in many classes of DNA-binding proteins.
authors.affiliation: Dipartimento di Scienze e Technologie Biomediche, Università di Udine, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Viglino
authors.forename: F
authors.initials: F
authors.lastname: Fogolari
authors.forename: S
authors.initials: S
authors.lastname: Formisano
authors.forename: N
authors.initials: N
authors.lastname: Bortolotti
authors.forename: G
authors.initials: G
authors.lastname: Damante
authors.forename: R
authors.initials: R
authors.lastname: Di Lauro
authors.forename: G
authors.initials: G
authors.lastname: Esposito
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000737:chemistry*; D017433:Protein Structure, Secondary; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry*
chemical_list: D004268:DNA-Binding Proteins; C000614416:Nkx2-1 protein, rat; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors