Secretion of bacterial beta-lactamase into culture broth by a mutant strain of Saccharomyces cerevisiae.
The secretion of Escherichia coli beta-lactamase was studied in a Saccharomyces cerevisiae ts1 mutant strain. The signal sequence of pre-beta-lactamase was recognized by the yeast cell and the precursor protein was processed to an enzymatically active mature beta-lactamase. In contrast to conventional strains, the mutant strain was able to secrete bla-gene-encoded beta-lactamase into the culture broth. These results indicate the potential usefulness of ts1 mutant strains for biotechnological purposes.
Bröker
M
M
Research Laboratories of Behringwerke AG, Marburg, Germany.
D001709:Biotechnology; D004926:Escherichia coli / Q000201:enzymology*; D015964:Gene Expression Regulation, Bacterial; D015151:Immunoblotting; D066298:In Vitro Techniques; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D001618:beta-Lactamases / Q000096:biosynthesis*
D001618:beta-Lactamases