Differential effects of interleukin-1 alpha and beta on the arachidonic acid cascade in human synovial cells and chondrocytes in culture.
The effects of interleukin-1 alpha and beta were tested on the [3H]-arachidonic acid release and the prostaglandin synthesis by human cultured synovial cells and chondrocytes. Both forms of interleukin-1 stimulated the arachidonic acid release but interleukin-1 beta was more potent than IL-1 alpha. Human synovial cells and chondrocytes synthesized three types of prostaglandins upon stimulation with interleukin-1 alpha or beta: prostaglandin E2, F2 alpha and 6-keto-prostaglandin F1 alpha. Regarding the synthesis of these prostaglandins, IL-1 beta was again more potent than IL-1 alpha. A comparison between interleukin-1-stimulated synovial cells and chondrocytes revealed neither significant quantitative nor qualitative differences in both the arachidonic acid release and the prostaglandin synthesis.
Knott
I
I
Unité de Biochimie Cellulaire, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Dieu
M
M
Burton
M
M
Lecomte
V
V
Remacle
J
J
Raes
M
M
D016718:Arachidonic Acid / Q000378:metabolism*; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D010741:Phospholipases A / Q000378:metabolism; D011453:Prostaglandins / Q000096:biosynthesis; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*
D007375:Interleukin-1; D011453:Prostaglandins; D016718:Arachidonic Acid; D010741:Phospholipases A