c-fos antisense generates apomorphine and amphetamine-induced rotation.
Sodium pentobarbital anaesthetized rats were injected ipsilaterally with an antisense oligonucleotide to c-fos and contralaterally with a sense of oligonucleotide to c-fos in the striatum. Ten hours later they were injected with amphetamine or apomorphine and their behaviour was observed for a further 2 h at which time they were overdosed with anaesthetic and their brains were removed, frozen and processed for Fos and Jun B immunohistochemistry. Rats showed amphetamine- and apomorphine-induced rotation towards the antisense injected striatum. The antisense oligonucleotide also strongly inhibited the amphetamine-induced expression of c-Fos and Jun B in striatal neurones. These results suggest that antisense to c-fos produces a biochemical change in the injected striatum that then, 10 h later, blocks amphetamine- and apomorphine-induced behavioural and biochemical effects.
Dragunow
M
M
Department of Pharmacology, School of Medicine, University of Auckland, New Zealand.
Lawlor
P
P
Chiasson
B
B
Robertson
H
H
D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D001483:Base Sequence; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos / Q000235:genetics*; D007267:Injections; D008297:Male; D008969:Molecular Sequence Data; D017072:Neostriatum; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012399:Rotation; D013239:Stereotyped Behavior / Q000187:drug effects*
D016376:Oligonucleotides, Antisense; D000661:Amphetamine; D001058:Apomorphine