Identification of a cryptic gene associated with an insertion sequence not previously identified in Bacillus thuringiensis.
After screening several Bt strains with a cryII toxin probe, clones from two strains were found to contain a cryptic cryIIB gene associated with an insertion sequence element belonging to the IS2/IS3 family. The lack of expression of this gene appears to result from mutation of the upstream orf2 gene which has been shown to be necessary for cryII expression.
Hodgman
T C
TC
Department of Biochemistry, University of Cambridge, UK.
Ziniu
Y
Y
Shen
J
J
Ellar
D J
DJ
D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis