Distinction between Aujeszky's disease virus-infected and vaccinated pigs by hemagglutination-inhibition test.
A hemagglutination-inhibition (HI) test was applied to distinguish virulent Aujeszky's disease virus infected pigs from those immunized with a glycoprotein gIII deletion vaccine. The vaccine strain, dlg92/dltk, did not have hemagglutination activity with mouse erythrocytes and the pigs vaccinated five times with the dlg92/dltk strain failed to develop HI antibody, although they developed neutralizing antibody with 128 to 512 titers to Aujeszky's disease virus. On the other hand, these pigs produced HI antibody 1 to 2 weeks after virulent virus inoculation. Thus the animals infected with virulent strain were easily differentiated from the animals immunized with the gIII deletion vaccine.
Shibata
I
I
Zen-noh Institute of Animal Health, Chiba, Japan.
Uruno
K
K
Samegai
Y
Y
Inaba
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007115:Immunization Schedule; D011557:Pseudorabies / Q000097:blood / Q000276:immunology* / Q000517:prevention & control; D013552:Swine; D014765:Viral Vaccines
D000914:Antibodies, Viral; D014765:Viral Vaccines