In vitro evaluation of ablation parameters of normal and fibrous aorta using smooth excimer laser coronary angioplasty.
A modified excimer laser energy delivery system was used to irradiate 100 segments of normal and fibrous aorta in vitro. The laser beam was scanned into 8 fiber bundles consisting of 50 fibers each resulting in a reduction of the applied pulse energy. The total repetition rate was increased to 150 Hz in order to keep the repetition rate per fiber bundle close to 20 Hz and to minimize thermal injury. The results demonstrate that effective ablation (etch rate per 8 pulses > 2.0 microns) occurred at an energy fluency of 50 mJ/mm2 in both normal and fibrous aorta. Tissue damage (carbonization, tissue separation, fissures, cracks, and vacuolization) was in a range of 100 +/- 28 to 152 +/- 30 microns for normal aorta and in a range of 57 +/- 35 to 110 +/- 39 microns for fibrous aorta. We conclude that effective ablation of normal and fibrous human aorta can be achieved by the application of smooth excimer laser coronary angioplasty. This improvement of excimer laser technology may result in a reduction of shock wave- and cavitation-induced damage leading to a reduction of tissue injury. However, this awaits further in vitro and in vivo confirmation.
Xie
D Y
DY
Department of Cardiology, University of Tübingen, Germany.
Hassenstein
S
S
Oberhoff
M
M
Hanke
H
H
Baumbach
A
A
Hohla
K
K
Haase
K K
KK
Karsch
K R
KR
D016342:Angioplasty, Laser / Q000379:methods*; D001011:Aorta / Q000473:pathology / Q000601:surgery*; D001018:Aortic Diseases / Q000473:pathology / Q000601:surgery; D001161:Arteriosclerosis / Q000473:pathology / Q000601:surgery*; D005069:Evaluation Studies as Topic; D006801:Humans; D066298:In Vitro Techniques; D011829:Radiation Dosage