title: High-performance liquid chromatographic microassay for L-glutamate:glyoxylate aminotransferase activity in human liver. Application in primary hyperoxaluria type 1.
abstract: A rapid and sensitive liquid chromatographic technique to determine L-glutamate:glyoxylate and aminotransferase (EC 2.6.1.4) activity in human liver is described. Homogenised tissue was incubated for 60 min in the presence of substrates and the 2-oxoglutarate generated was converted into the corresponding phenylhydrazone which was determined using reversed-phase high-performance liquid chromatography. The procedure allowed the detection of the enzyme activity expressed by 7.5 micrograms of liver protein, it was more sensitive and less time-consuming than the spectrophotometric procedure previously used. No significant differences were found between normal controls and patients with primary hyperoxaluria. In an 8-month-infant with primary hyperoxaluria type 1, the enzyme activity was reduced to 16% of the average control values.
authors.affiliation: Laboratorio Calcolosi Renale, Ospedale Mauriziano Umberto I, Turin, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Petrarulo
authors.forename: S
authors.initials: S
authors.lastname: Pellegrino
authors.forename: M
authors.initials: M
authors.lastname: Marangella
authors.forename: D
authors.initials: D
authors.lastname: Cosseddu
authors.forename: F
authors.initials: F
authors.lastname: Linari
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006960:Hyperoxaluria, Primary / Q000201:enzymology*; D007223:Infant; D008099:Liver / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D010659:Phenylhydrazines; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D000637:Transaminases / Q000378:metabolism*
chemical_list: D010659:Phenylhydrazines; C030299:phenylhydrazine; D000637:Transaminases; C020649:glutamate-glyoxylate aminotransferase