title: Rapid enzyme immunoassay of anti-streptokinase antibodies in human plasma.
abstract: A simple enzyme immunoassay for determination of anti-streptokinase antibodies (aSKa) in plasma is described. Commercially available reagents have been used for the assay, which is calibrated with a reference preparation of aSKa containing 100 AU/ml. The assay is specific and reproducible with a variation coefficient of 4.8%. In healthy individuals a broad range of values between 4 and 291 AU/ml was observed with a large difference between the mean and median value (55 AU/ml and 27 AU/ml, respectively). Data from a study on 21 patients with myocardial infarction treated with the streptokinase derivative antistreplase suggest that a high titre of aSKa before treatment is associated with failure of thrombolytic therapy. The assay procedure can be shortened to 0.5 h to screen patients for a high aSKa level. This assay allows a more routine assessment of aSKa in the clinic.
authors.affiliation: Department of Haemostasis, Thrombosis & Rheology, University Hospital Groningen, The Netherlands.
authors.forename: V J
authors.initials: VJ
authors.lastname: Bom
authors.forename: J
authors.initials: J
authors.lastname: Brügemann
authors.forename: W
authors.initials: W
authors.lastname: van der Schaaf
authors.forename: R T
authors.initials: RT
authors.lastname: van Wijk
authors.forename: J
authors.initials: J
authors.lastname: van der Meer
mesh_terms: D016255:Anistreplase / Q000627:therapeutic use; D000906:Antibodies / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D009203:Myocardial Infarction / Q000097:blood / Q000188:drug therapy / Q000276:immunology; D013300:Streptokinase / Q000276:immunology*
chemical_list: D000906:Antibodies; D016255:Anistreplase; D013300:Streptokinase