S-D-lactoylglutathione accumulation in activated human platelets.
1. S-D-Lactoylglutathione accumulates in human platelets activated by agonists. Among the tested inducers thrombin is the most active. 2. The effect is dose and time-dependent. S-D-Lactoylglutathione, corresponding to depleted pool of reduced glutathione, can also be detected in platelets incubated with exogenous methylglyoxal. 3. A further significant increase was observed in platelets stimulated with thrombin in the presence of methylglyoxal. 4. No change in glyoxalase activities upon platelet stimulation with thrombin was shown.
Leoncini
G
G
Istituto Policattedra di Chimica Biologica, Università degli Studi di Genova, Italy.
Buzzi
E
E
Aprile
B
B
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007791:Lactoylglutathione Lyase / Q000378:metabolism; D010972:Platelet Activating Factor / Q000494:pharmacology; D015539:Platelet Activation; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013869:Thiolester Hydrolases / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D000099301:Hydroxyacylglutathione Hydrolase
D000001:Calcimycin; D005978:Glutathione; D007791:Lactoylglutathione Lyase; D010972:Platelet Activating Factor; D013755:Tetradecanoylphorbol Acetate; D013869:Thiolester Hydrolases; D013917:Thrombin; D000099301:Hydroxyacylglutathione Hydrolase; C013585:S-lactoylglutathione