title: Repeatability of energy expenditure measurements in clinically normal dogs by use of indirect calorimetry.
abstract: Energy expenditure (EE) was determined, using an open-flow indirect calorimetry system in a group of 20 clinically normal, apparently resting, client-owned dogs. Five evaluations were performed over an 8-hour period to determine reliability of the method. The intraclass correlation coefficient was calculated as the ratio of within- and between-subject variances, using repeated-measures ANOVA. When only the middle 3 evaluations were included, the intraclass correlation coefficient was 0.87, indicating good reliability. The first evaluation was higher than the subsequent 4 evaluations for rate of O2 consumption (Vo2/kg and Vo2/kg0.75; (P < or = 0.01), and EE/kg and EE/kg0.75 (P < or = 0.005). The respiratory quotients at the first (P = 0.004) and second (P = 0.013) evaluations were different from the respiratory quotient at the fourth evaluation. Therefore, the first evaluation may not be representative of the actual EE. The mean value of at least 3 subsequent evaluations after an adequate adaptation period (5 to 10 minutes) to the equipment will be useful for predicting energy requirements of apparently resting, clinically normal dogs.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
authors.forename: L M
authors.initials: LM
authors.lastname: Walters
authors.forename: G K
authors.initials: GK
authors.lastname: Ogilvie
authors.forename: M D
authors.initials: MD
authors.lastname: Salman
authors.forename: L
authors.initials: L
authors.lastname: Joy
authors.forename: M J
authors.initials: MJ
authors.lastname: Fettman
authors.forename: M S
authors.initials: MS
authors.lastname: Hand
authors.forename: S L
authors.initials: SL
authors.lastname: Wheeler
mesh_terms: D000818:Animals; D002153:Calorimetry, Indirect / Q000706:statistics & numerical data / Q000662:veterinary*; D004285:Dogs / Q000378:metabolism*; D004734:Energy Metabolism; D005069:Evaluation Studies as Topic; D005260:Female; D008297:Male; D010101:Oxygen Consumption; D015203:Reproducibility of Results