title: Responses to nutrient starvation in Pseudomonas putida KT2442: analysis of general cross-protection, cell shape, and macromolecular content.
abstract: The physiology of Pseudomonas putida KT2442 with respect to growth and carbon starvation was studied. During the transition from growth to nongrowth, the cell shape changes from cylindrical to spheric, a change which is accompanied by reductions in cell size, DNA and ribosome content, and the rate of total protein synthesis. In addition, a pattern of general cross-protection develops, which enables the cells to survive environmental stresses such as high and low temperatures, elevated osmolarity, solvents, and oxidative agents. Cultures are almost fully viable during 1 month of carbon, nitrogen, and multiple-nutrient starvation and are considered to be in an active nondormant state. In contrast, strain KT2442 does not survive well under conditions of sulfate and phosphate starvation.
authors.affiliation: Department of Microbiology, Technical University of Denmark, Lyngby.
authors.forename: M
authors.initials: M
authors.lastname: Givskov
authors.forename: L
authors.initials: L
authors.lastname: Eberl
authors.forename: S
authors.initials: S
authors.lastname: Møller
authors.forename: L K
authors.initials: LK
authors.lastname: Poulsen
authors.forename: S
authors.initials: S
authors.lastname: Molin
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000096:biosynthesis; D002244:Carbon / Q000378:metabolism; D002455:Cell Division; D004269:DNA, Bacterial / Q000032:analysis; D004361:Drug Tolerance; D000431:Ethanol / Q000494:pharmacology; D005947:Glucose / Q000172:deficiency*; D009584:Nitrogen / Q000172:deficiency; D009997:Osmotic Pressure; D010084:Oxidation-Reduction; D010710:Phosphates / Q000172:deficiency; D016958:Pseudomonas putida / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012270:Ribosomes / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010710:Phosphates; D013431:Sulfates; D000431:Ethanol; D002244:Carbon; D005947:Glucose; D009584:Nitrogen