Restriction map of a virulence-associated plasmid of Rhodococcus equi.
The restriction cleavage map of a virulence-associated plasmid pREAT701 of Rhodococcus equi was constructed with EcoRI and HindIII by means of cloning the restriction fragments, cross Southern hybridization with each fragment, and hybridization with probes generated by modified inverse PCR. The genetic region responsible for expression of virulence-associated 15- to 17-kilodalton antigens was determined.
Kanno
T
T
National Institute of Animal Health, Ibaraki, Japan.
Asawa
T
T
Ito
H
H
Takai
S
S
Tsubaki
S
S
Sekizaki
T
T
D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016929:Rhodococcus equi / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D017931:DNA Primers