[Effect of chorionic gonadotropin on formation of secondary immune response].
Experiments were carried out in vivo and in vitro. Secondary immune response to sheep red cells was assessed on day 5 after repeated immunization by IgM and IgG antibody-producing cells. Chorionic gonadotropin was injected after reimmunization to intact and ovariectomized animals in doses compatible to those in normal pregnancy. In vitro chorionic gonadotropin was added for one h into sensitized splenocyte cultures and then its effects were assessed in syngeneic transfer system by the same parameters. Experiments have demonstrated that in dose 40 MU chorionic gonadotropin cannot influence secondary immune response formation. All hormonal effects are related to 200 MU dose extrapolated from the maximal level of this hormone during pregnancy. In this dose gonadotropin reduced splenocyte counts and inhibited the formation of IgG-antibody-producing cells. Ovarian sex steroids do not mediate chorionic gonadotropin immunomodulating effects. An hour's contact of sensitized splenocytes with the hormone in a high dose resulted in a statistically significant inhibition of the formation of IgM-antibody-producing cells. Hence, if chorionic gonadotropin interacted with immunocompetent cells before their repeated contact with the antigen, IgM-antibody-producing cells suffer, and if this exposure occurs after this contact, IgG-antibody-producing cells decrease in number. The role of chorionic gonadotropin in development of normal pregnancy and that complicated by immunopathologic processes is discussed.
Shirshev
S V
SV
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005260:Female; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D010053:Ovary / Q000502:physiology; D011247:Pregnancy; D013256:Steroids / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D006063:Chorionic Gonadotropin; D012739:Gonadal Steroid Hormones; D013256:Steroids
Vliianie khorionicheskogo gonadotropina na formirovanie vtorichnogo immunnogo otveta.