Reduction of HTLV-I-infected cells in blood by leukocyte filtration.
Leukocyte filtration was performed with HTLV-I-infected blood and with blood supplemented with cultured HTLV-I-transformed cells. Reduction of infectivity upon leukocyte filtration was determined by the polymerase chain reaction (PCR) using primers indicative for the HTLV-I-pol and tax genes. Two different commercially available filters were used: a column-shaped cellulose acetate and a flat-bed polyester filter. Both filters yielded reduction of at least 3 10logs for cultured HTLV-I-infected cells. When blood from HTLV-I-infected individuals was used for filtration, the number of infected cells was reduced by 1-3 10logs. Although filtered blood as yet cannot be regarded as safe, it is concluded that leukocyte filtration of HTLV-I-infected blood potentially contributes to reducing the spread of HTLV-I by blood transfusion.
Al
E J
EJ
Department of Development Research, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Visser
S C
SC
Broersen
S M
SM
Stienstra
S
S
Huisman
J G
JG
D001769:Blood / Q000382:microbiology*; D001782:Blood Donors; D001800:Blood Specimen Collection / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D017931:DNA Primers; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration / Q000379:methods; D016355:Genes, pX; D015751:Genes, pol; D015481:HTLV-I Antibodies / Q000097:blood; D015490:HTLV-I Infections / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004279:DNA, Viral; D015481:HTLV-I Antibodies