[Anoxia-reperfusion injury to endothelial cells: mechanism and protection].
Endothelial cells in the aortic walls of inbred SD rats were cultured in MEM culture medium containing 10% calf serum, and an anoxia-reperfusion injury model was established. The effect of anoxia-reperfusion injury on cultured endothelial cells was studied by measurements of membrane fluidity, intracellular Ca content, the release rate of 51Cr and the uptake rate of trypan-blue. The effect of fructose 1-6 diphosphate (FDP) and captopril (Cap) on cultured endothelial cells was also investigated. The findings indicated that the two drugs might protect cultured endothelial cells from anoxia-reperfusion injury. The mechanism of anoxia-reperfusion injury and the protective effect of the two drugs on cultured endothelial cells were briefly discussed.
Yu
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Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xian.
D000818:Animals; D001011:Aorta / Q000166:cytology; D002118:Calcium / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology*; D015687:Cell Hypoxia / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005635:Fructosediphosphates / Q000494:pharmacology*; D008560:Membrane Fluidity; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
D005635:Fructosediphosphates; D002216:Captopril; C029063:fructose-1,6-diphosphate; D002118:Calcium