Inhibition of lymphocyte adherence to rat lacrimal acinar epithelium by interleukin-4 and transforming growth factor-beta 1.
Mature circulating lymphocyte populations specifically bind to lacrimal gland acinar epithelium in vitro and this adherence is thought to contribute to the accumulation of lymphoid subsets within lacrimal tissue in vivo. The regulatory role of interleukin-4 (IL-4) and transforming growth factor-beta (TGF-beta) in this adherence process was examined using an in vitro binding assay. Pretreatment of thoracic duct lymphocytes (TDLs) with increasing concentrations of IL-4 or TGF-beta for 1 hr resulted in a dose-dependent inhibition of lymphocyte binding to lacrimal gland acinar epithelium. In contrast, the binding of TDLs to high endothelial venules of cervical lymph node was not inhibited by either cytokine. Further, IL-4 and TGF-beta pretreatment did not alter the expression of lymph node or Peyer's patch homing receptors as well as the LFA-1, VLA-4, or CD44 adhesion molecules on TDLs. These results suggest that the interaction of lymphocytes with lacrimal gland acinar epithelium may be regulated by a receptor-mediated mechanism that differs from those governing HEV recognition.
Elfaki
M G
MG
Department of Immunology and Microbiology, Wayne State University, School of Medicine, Detroit, Michigan 48201.
O'Sullivan
N L
NL
Skandera
C A
CA
Montgomery
P C
PC
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D015847:Interleukin-4 / Q000494:pharmacology*; D007765:Lacrimal Apparatus / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D013897:Thoracic Duct; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D015815:Cell Adhesion Molecules; D016201:Receptors, Lymphocyte Homing; D016212:Transforming Growth Factor beta; D015847:Interleukin-4