[Enzymes of ethanol metabolism in the liver of rats with varying alcohol motivation in acute alcoholization].
Alcohol dehydrogenase (ADH) activity does not change in ethanol-preferring rats (EP receiving ethanol injection in a dose of 2.5 g/kg; an increase of the dose to 4.5 g/kg leads to a descent of activity 60 min later. Enhancement of the reverse reaction activity 120 min after injection of ethanol both doses probably induce faster metabolism of acetylanhydride and thus absence of the adverse ethanol action in these animals. An increase of activity of NAD(+)- and NADP(+)-dependent isoforms of ALDH-II 60 min after their injection in a dose of 2.5 and 4.5 g/kg also contributes to this effect. Synchronous changes both in direct and reverse ADH reactions in water-preferring (WP) rats with 2.5 g/kg ethanol injection were observed: the activity increase 30 min later followed by normalization at the 60th min and a repeated increase 120 min later; a dose of 4.5 g/kg induced an increase of only direct ADH reaction activity at the 30th and 60th min. The liver mitochondria ALDH-I of WP were practically unsusceptible to ethanol in a dose of 2.5 and 4.5 g/kg. Activity of NAD(+)-dependent ALDH-II mitochondria decreased with 2.5 g/kg at the 30th and 60th min, respectively. Yet an increase in activity of isoforms in microsomes was observed. ADH activity in short-sleeping (SS) rats does not change both at 2.5 and at 4.5 g/kg doses. More intensive ethanol metabolism in these rats is probably connected with increased activity of MEOS and catalase.(ABSTRACT TRUNCATED AT 250 WORDS)
Konoplitskaia
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Mnyshenko
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Zakashun
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D000426:Alcohol Dehydrogenase / Q000187:drug effects; D000444:Aldehyde Dehydrogenase / Q000187:drug effects; D000818:Animals; D004361:Drug Tolerance; D000431:Ethanol / Q000008:administration & dosage / Q000378:metabolism*; D007527:Isoenzymes / Q000187:drug effects; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology; D009042:Motivation; D051381:Rats; D013997:Time Factors
D007527:Isoenzymes; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase
Fermenty obmena étanola pecheni krys s razlichnoĭ alkogol'noĭ motivatsieĭ pri ostroĭ alkogolizatsii.