Cloning of the cDNA (DSC1) coding for human type 1 desmocollin and its assignment to chromosome 18.
Desmosomes are adhesive epithelial junctions that contain two distinct classes of cadherin-related glycoproteins (desmogleins and desmocollins), both of which occur as several different isoforms whose expression is related to epithelial differentiation. We have now isolated cDNA clones encoding a human desmocollin that is expressed in the more differentiated layers of human epidermis. This isoform has 53% amino acid identity with the previously isolated human (type 3) desmocollin, which is expressed in the basal layers of the epidermis. However, the N- and C-termini of the mature proteins are more highly conserved. Using a panel of somatic cell hybrids, human type 1 desmocollin (gene DSC1) has been assigned to chromosome 18, the same location as the other desmocollin gene (DSC3) and the three desmoglein (DSG) genes already mapped.
King
I A
IA
Laboratory of Eukaryotic Molecular Genetics, National Institute for Medical Research, Mill Hill, London, United Kingdom.
Arnemann
J
J
Spurr
N K
NK
Buxton
R S
RS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002887:Chromosomes, Human, Pair 18; D003001:Cloning, Molecular; D004247:DNA; D051187:Desmocollins; D003896:Desmosomes / Q000378:metabolism*; D006801:Humans; D006822:Hybrid Cells; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
C081560:DSC1 protein, human; D051187:Desmocollins; D008562:Membrane Glycoproteins; D004247:DNA