False DNA aneuploidy in canine and human neoplasms.
In most cases, the appearance of aneuploid peaks in DNA histograms may be an artefact of tissue preparation or it may reflect non-stoichiometric dye binding of a cellular subpopulation rather than true DNA aneuploidy. This report reviews how false DNA aneuploidy can be recognized and eliminated from sample submitted for DNA flow cytometric analysis.
Deitch
A D
AD
Department of Urology, University of California, Davis 95817.
de Vere White
R W
RW
Madewell
B R
BR
D000782:Aneuploidy; D000818:Animals; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004283:Dog Diseases; D004285:Dogs; D005189:False Positive Reactions; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D009369:Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000662:veterinary*
D004273:DNA, Neoplasm