The same 15 kDa proteolipid subunit is a constituent of two different proteins in Torpedo, the acetylcholine releasing protein mediatophore and the vacuolar H+ ATPase.
Using the monoclonal antibody 15K1, we have studied, at the cellular and subcellular levels, the distribution of a 15 kDa proteolipid, identified as the subunit of mediatophore, a presynaptic membrane protein able to release acetylcholine when activated by calcium. Aside from the electric lobe, the antigen distribution in the brain of Torpedo paralleled that of the synaptic vesicle antigen SV2 and did not appear to be related to that of acetylcholine and choline acetyltransferase. The 15 kDa proteolipid antigen was therefore present in all nerve endings and not restricted to cholinergic ones. At the ultrastructural level, on cholinergic nerve endings, the antigen was detected associated to synaptic vesicles and, to a lesser extent, to the presynaptic plasma membrane. Indeed, considering the high sequence homology between the mediatophore subunit (Birman et al., 1990) and the proteolipid subunit of the vacuolar type H+ ATPase, a major enzyme constituent of synaptic vesicles, this distribution was not surprising. To determine whether antibody 15K1 recognizes the vacuolar type H+ ATPase, we chose a non neuronal cell type which possesses a high content of this enzyme, the kidney proton secreting epithelial cells. Indeed, antibody 15K1 intensely labelled the apical plasma membrane of mitochondria rich epithelial cells in kidney tubules. A high density of the antigen was also found associated to intracellular membrane structures such as lysosomal multivesicular bodies, both in kidney epithelial cells and in electromotoneurons. The 15 kDa proteolipid antigen was associated with other vacuolar H+ ATPase subunits in kidney membranes which was not the case in presynaptic plasma membranes. This illustrates that the 15 kDa proteolipid antigen is a constituent of two different protein complexes, which exhibit very different functional properties.
Brochier
G
G
Department Neurochimie, Laboratoire Neurobiologie Cellulaire et Moleculaire C.N.R.S., Gif sur Yvette, France.
Morel
N
N
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004557:Electric Organ / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007668:Kidney / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D011510:Proteolipids / Q000032:analysis* / Q000737:chemistry; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000378:metabolism; D014018:Tissue Distribution; D014101:Torpedo / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology
D000911:Antibodies, Monoclonal; D009419:Nerve Tissue Proteins; D011510:Proteolipids; C050299:mediatophore; D002795:Choline O-Acetyltransferase; D006180:Proton-Translocating ATPases; D000109:Acetylcholine