title: Agarose gel immunodiffusion tests with the addition of PEG (polyethylene glycol 6000) for the diagnosis and serological follow up of paracoccidioidomycosis infection.
abstract: By using PEG (polyethylen glycol 6000) in the gel immunodiffusion tests (ID), the precipitin lines were increased in 25.5% of the 192 sera reactions and the titers were increased from one to four serial dilutions in 44.6% of the 139 serum samples. Owing to its sensitivity, easy interpretation of the results and low cost, the use of 2% PEG incorporated into the gel in ID tests is recommended for the diagnosis and serological follow-up of paracoccidioidomycosis infections.
authors.affiliation: Setor de Micologia Médica, Escola Paulista de Medicina, São Paulo, Brasil.
authors.forename: M H
authors.initials: MH
authors.lastname: Forjaz
authors.forename: O
authors.initials: O
authors.lastname: Fischman
authors.forename: L
authors.initials: L
authors.lastname: Zaror
authors.forename: N F
authors.initials: NF
authors.lastname: Yonashiro
mesh_terms: D005500:Follow-Up Studies; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*; D010229:Paracoccidioidomycosis / Q000175:diagnosis* / Q000276:immunology; D011092:Polyethylene Glycols; D012680:Sensitivity and Specificity; D012685:Sepharose
chemical_list: D011092:Polyethylene Glycols; D012685:Sepharose