title: Loss of heterozygosity on chromosome 13 is common only in the biologically more aggressive subtypes of ovarian epithelial tumors and is associated with normal retinoblastoma gene expression.
abstract: We examined the frequencies of loss of heterozygosity on chromosome 13 in 77 primary ovarian epithelial tumors subdivided into cystadenomas, tumors of low malignant potential, low grade carcinomas, and high grade carcinomas. Such losses were found in approximately 50% of high grade carcinomas but were not detected in any of the other tumor subtypes (P < 0.0001), suggesting a strong association between these abnormalities and the high grade carcinoma phenotype. The tumors were also examined for abnormalities in expression of the retinoblastoma susceptibility gene (RB). This was assessed by immunohistochemical staining of archival tumor sections with a polyclonal antibody directed against both the phosphorylated and the underphosphorylated forms of the RB protein. Most of the tumors, including those with allelic deletions on chromosome 13, showed normal RB nuclear protein staining patterns. We conclude that loss of RB expression is not a frequent event in primary ovarian carcinomas and that this gene is probably not the target of the frequent allelic deletions on chromosome 13 found in high grade ovarian carcinomas.
authors.affiliation: Department of Pathology, Kenneth Norris Jr. Comprehensive Cancer Center, USC School of Medicine, Los Angeles 90033.
authors.forename: T M
authors.initials: TM
authors.lastname: Kim
authors.forename: W F
authors.initials: WF
authors.lastname: Benedict
authors.forename: H J
authors.initials: HJ
authors.lastname: Xu
authors.forename: S X
authors.initials: SX
authors.lastname: Hu
authors.forename: J
authors.initials: J
authors.lastname: Gosewehr
authors.forename: M
authors.initials: M
authors.lastname: Velicescu
authors.forename: E
authors.initials: E
authors.lastname: Yin
authors.forename: J
authors.initials: J
authors.lastname: Zheng
authors.forename: G
authors.initials: G
authors.lastname: D'Ablaing
authors.forename: L
authors.initials: L
authors.lastname: Dubeau
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics; D002882:Chromosomes, Human, Pair 13 / Q000502:physiology*; D003537:Cystadenoma / Q000235:genetics; D004848:Epithelium / Q000473:pathology; D005260:Female; D017353:Gene Deletion; D015870:Gene Expression / Q000235:genetics*; D016161:Genes, Retinoblastoma / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology*; D016160:Retinoblastoma Protein / Q000032:analysis / Q000235:genetics
chemical_list: D016160:Retinoblastoma Protein