A PCR method for the sequence analysis of the gyrA, polA and rnhA gene segments from mycobacteria.
Internal segments of the gyrA and polA genes involved in DNA replication of Mycobacterium tuberculosis and rnhA of M. smegmatis, have been amplified by the polymerase chain reaction (PCR) using degenerate oligodeoxyribonucleotide primers based on conserved sequences. The deduced amino acid sequences were 54-66% homologous to the corresponding segments of their Escherichia coli counterparts. This method provides a useful means of cloning genes encoding DNA replication enzymes of mycobacteria.
Mizrahi
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Molecular Biology Unit, South African Institute for Medical Research, Johannesburg.
Huberts
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Dawes
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Dudding
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D000595:Amino Acid Sequence; D001483:Base Sequence; D004256:DNA Polymerase I / Q000235:genetics*; D017931:DNA Primers; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016914:Ribonuclease H / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers; D004256:DNA Polymerase I; D016914:Ribonuclease H; C078856:ribonuclease HI; D004250:DNA Topoisomerases, Type II