Cytotoxicity potential of surfactant mixtures evaluated by primary cultures of rabbit corneal epithelial cells.
The use of in vitro cytotoxicity assays as potential alternatives in assessing ocular irritation of surfactant mixtures was evaluated in a primary culture system of rabbit corneal epithelial cells. Two groups of surfactant mixtures, each with the same surfactant components in varying proportions, were studied. Cytotoxicity was determined by lactate dehydrogenase (LDH) enzyme leakage and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye reduction in the cell culture system. There was a good correlation between the cytotoxicity in vitro and the reported Draize eye irritation data within each group of the surfactant mixtures studied.
Yang
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Division of Pharmacology and Toxicology, College of Pharmacy, University of Texas at Austin 78712.
Acosta
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D
D000826:Animal Testing Alternatives; D000818:Animals; D002478:Cells, Cultured; D004396:Coloring Agents / Q000378:metabolism; D003315:Cornea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D010084:Oxidation-Reduction; D011817:Rabbits; D013501:Surface-Active Agents / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D004396:Coloring Agents; D013501:Surface-Active Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue