title: Direct detection of PCR products for HLA class II typing.
abstract: Direct detection of the PCR, or DD-PCR is proposed as an efficient method for performing PCR assays. Following the PCR reaction, ethidium homodimer dye is added to the reaction mixture and read by fluorescence. The dye step circumvents the necessity of running reactions on agarose gel electrophoresis, which is the current standard. This simple modification should find wide application for assays utilizing the PCR reaction. Here we show the ready detection of HLA class II polymorphism.
authors.affiliation: Department of Surgery, UCLA School of Medicine.
authors.forename: D
authors.initials: D
authors.lastname: Chia
authors.forename: P
authors.initials: P
authors.lastname: Terasaki
authors.forename: H
authors.initials: H
authors.lastname: Chan
authors.forename: R
authors.initials: R
authors.lastname: Tonai
authors.forename: P A
authors.initials: PA
authors.lastname: Siauw
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005456:Fluorescent Dyes; D005470:Fluorometry; D005802:Genes, MHC Class II; D006681:HLA-D Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D006681:HLA-D Antigens; D004996:Ethidium