title: Genomic PCR-SSCP analysis of the metastasis associated NM23-H1 (NME1) gene: a study on colorectal cancer.
abstract: To facilitate further mutational analysis of NM13-H1, a human metastasis suppressor gene, we have established its genomic organization. NM23-H1 is composed of five exons, spanning a genomic DNA fragment of 10 kb. Using oligonucleotide primers flanking each exon, PCR-SSCP analysis was performed on genomic DNAs of healthy individuals. A common polymorphism, a C to T transition, was detected 30 nucleotides upstream from the 5' splice site flanking exon 1. As NM23-H1 allele loss and altered expression have been reported in colorectal cancer, genomic DNAs of 20 colorectal tumors were analyzed for the presence of gene-specific mutations by PCR-SSCP: no abnormal sequences were detected within the coding and splice site regions of the NM23-H1 gene. This finding suggests that NM23-H1 mutations are rare events in human colorectal cancer.
authors.affiliation: Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bafico
authors.forename: L
authors.initials: L
authors.lastname: Varesco
authors.forename: L
authors.initials: L
authors.lastname: De Benedetti
authors.forename: M A
authors.initials: MA
authors.lastname: Caligo
authors.forename: V
authors.initials: V
authors.lastname: Gismondi
authors.forename: S
authors.initials: S
authors.lastname: Sciallero
authors.forename: H
authors.initials: H
authors.lastname: Aste
authors.forename: G B
authors.initials: GB
authors.lastname: Ferrara
authors.forename: G
authors.initials: G
authors.lastname: Bevilacqua
mesh_terms: D001483:Base Sequence; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D003360:Cosmids; D004252:DNA Mutational Analysis; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D005091:Exons; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009362:Neoplasm Metastasis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012326:RNA Splicing; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary