K+ ionophores inhibit nerve growth factor-induced neuronal differentiation in rat adrenal pheochromocytoma PC12 cells.
Incubation with a K+/H+ ionophore nigericin attenuated the nerve growth factor (NGF)-induced neurite outgrowth in rat pheochromocytoma PC12 cells. However, a Na+/H+ ionophore monensin and a Ca2+ ionophore A23187 did not inhibited the neurite outgrowth. Nigericin also inhibited the NGF-caused induction of acetylcholinesterase and suppression of cell proliferation. These changes were dependent on the amount of the ionophore added to the culture. In addition, a distinct K+ ionophore, valinomycin, similarly inhibited the NGF-induced neuronal differentiation. These results suggest the presence of the K+ ionophore-sensitive mechanism in the NGF-induced differentiation system in PC12 cells.
Harada
H
H
Department of Environmental Biochemistry and Toxicology, University of Shizuoka, School of Pharmaceutical Science, Japan.
Morita
M
M
Suketa
Y
Y
D000110:Acetylcholinesterase / Q000378:metabolism; D000310:Adrenal Gland Neoplasms; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008985:Monensin / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D009550:Nigericin / Q000494:pharmacology*; D016716:PC12 Cells; D010673:Pheochromocytoma; D011188:Potassium / Q000378:metabolism; D051381:Rats; D014634:Valinomycin / Q000494:pharmacology*
D009414:Nerve Growth Factors; D014634:Valinomycin; D000001:Calcimycin; D008985:Monensin; D000110:Acetylcholinesterase; D009550:Nigericin; D011188:Potassium