Interferon-gamma severely inhibits DNA synthesis of vaccinia virus in a macrophage cell line.
Interferon-gamma inhibits the growth of several viruses; however, the exact mechanism of this inhibition has not been clearly defined. In this report, we show that in a monocyte/macrophage cell line J774.G8 IFN-gamma inhibits the growth of vaccinia virus (VV) in a dose-dependent manner. This inhibition is due to a block in VV DNA synthesis and correlates with an increased generation of reactive nitrogen intermediates.
Mĕlková
Z
Z
Department of Biochemistry, State University of New York, Brooklyn 11203.
Esteban
M
M
D000818:Animals; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D014616:Vaccinia virus / Q000254:growth & development*
D004279:DNA, Viral; D009569:Nitric Oxide; D007371:Interferon-gamma