Enzyme-linked immunosorbent assay for antibodies to ZEBRA, an Epstein-Barr trans-activator.
Patients with AIDS and AIDS-related complex often show symptoms of Epstein-Barr virus (EBV) reactivation. Several EBV-encoded trans-acting factors activate the EBV lytic cycle, and one, called ZEBRA (BamHI Z EBV replication activator), controls the switch of EBV from a latent to a productive cycle. We describe here a simple ELISA test using a bacterially expressed ZEBRA protein as antigen. Utilizing this technique, we evaluated the humoral response to ZEBRA antigen in 38 patients with nasopharyngeal carcinoma and 134 subjects with an asymptomatic HIV 1 infection. The control group consisted of 40 healthy adult blood donors.
Maréchal
V
V
Laboratoire de Microbiologie, Hôpital Rothschild, Paris, France.
Meyohas
M C
MC
Joab
I
I
Gaha
S
S
Giot
J F
JF
Sergeant
A
A
Nicolas
J C
JC
D000914:Antibodies, Viral / Q000097:blood*; D004268:DNA-Binding Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015658:HIV Infections / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology; D015534:Trans-Activators / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; C063676:BZLF1 protein, Herpesvirus 4, Human; D004268:DNA-Binding Proteins; D015534:Trans-Activators; D014764:Viral Proteins