Effects of trehalose in canine lung preservation.
BACKGROUND
The effect of trehalose on the preservation of canine lungs was studied with the use of Euro-Collins solution (ECS).


METHODS
In group 1, five lungs were perfused and preserved with a modified ECS in which trehalose (35.0 gm/L) was used instead of glucose. In group 2, six lungs were perfused and preserved with a modified ECS in which glucose was replaced by 70.0 gm/L trehalose. In group 3, six lungs were perfused and preserved with ECS. After preservation for 12 hours, left lung transplantation was performed.


RESULTS
The PaO2 values in groups 1 and 2 at 130 minutes after reperfusion were 264.9 and 257.5 mm Hg, respectively. These PaO2 values were significantly higher than the corresponding PaO2 value in group 3 (114.8 mm Hg). All the transplanted lungs in groups 1 and 2 had normal structures on histologic examination, whereas five of the group 3 lungs showed severe pulmonary edema.


CONCLUSIONS
These findings show that trehalose is effective in the preservation of lungs for 12 hours.
Hirata
T
T
Department of Thoracic Surgery, Kyoto University, Japan.
Fukuse
T
T
Liu
C J
CJ
Muro
K
K
Yokomise
H
H
Yagi
K
K
Inui
K
K
Hitomi
S
S
Wada
H
H
D000818:Animals; D004285:Dogs; D006439:Hemodynamics / Q000187:drug effects; D006982:Hypertonic Solutions; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D009926:Organ Preservation; D010100:Oxygen / Q000097:blood; D014199:Trehalose / Q000494:pharmacology*
C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D014199:Trehalose; D010100:Oxygen