A centrifugal separation procedure detects moderate affinity cGMP binding sites in membrane-associated proteins and permeabilized cells.
Forget
R S
RS
Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham 03824-3544.
Martin
J E
JE
Cote
R H
RH
D000818:Animals; D001001:Anura; D001665:Binding Sites; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002463:Cell Membrane Permeability; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation; D006152:Cyclic GMP / Q000302:isolation & purification / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D007700:Kinetics; D008565:Membrane Proteins / Q000302:isolation & purification*; D012243:Rhodopsin / Q000302:isolation & purification; D012374:Rod Cell Outer Segment / Q000737:chemistry
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C022994:cyclic GMP-binding protein; D012243:Rhodopsin; D006152:Cyclic GMP