title: Augmentation of portal blood flow improves function of human cirrhotic liver.
abstract: In cirrhotic livers, the intrahepatic resistance is increased and drug elimination and portal transhepatic flow are decreased. The aim of our work was to study the effect of a twofold increase in portal blood flow during 2 hr on the hemodynamic parameters, drug elimination and hepatic viability in eight isolated perfused human cirrhotic livers. Using an oxygenated recirculating system with independent arterial and portal flows, we perfused livers with Kreb's buffer bicarbonate solution, bovine serum albumin (20 gm.L-1) and human red blood cells (hematocrit 20%). The flow was maintained at a basal level of 0.713 +/- 0.19 L/min for 1 hr and then increased and maintained for 2 hr at twice the basal flow. Portal pressure-portal flow curve slopes were linear (27.04 +/- 21.06 mm Hg.L-1 x min; range = 6.43 to 60.8) and correlated with intrahepatic resistance during the basal-flow period (r = 0.87, p < 0.01). Parameters registered during the basal- and high-flow periods were compared by use of Student's t test: portal pressure increased from 23.5 +/- 7 to 37.3 +/- 16.7 mm Hg (p < 0.05); arterial pressure increased from 80.3 +/- 19 to 103.5 +/- 26 mm Hg (p < 0.005); hepatic artery flow resistance increased 31.9% (from 690.1 +/- 218 to 899.4 +/- 269 mm Hg.L-1 x min; p < 0.005); indocyanine green clearance increased by 28.2% (from 86.0 +/- 58.3 to 109.2 +/- 74.8 ml.min-1 x kg liver-1; p < 0.04). No significant differences were observed in enzyme release, biliary flow (n = 5) and oxygen consumption. Histological examinations demonstrated sinusoidal dilatations in six of eight cases.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratoire de Recherche Chirurgicale, Faculté de Médecine Cochin Port-Royal, Université Paris V, France.
authors.forename: J E
authors.initials: JE
authors.lastname: Cardoso
authors.forename: C
authors.initials: C
authors.lastname: Gautreau
authors.forename: P R
authors.initials: PR
authors.lastname: Jeyaraj
authors.forename: D
authors.initials: D
authors.lastname: Patrzalek
authors.forename: B
authors.initials: B
authors.lastname: Cherruau
authors.forename: M
authors.initials: M
authors.lastname: Vaubourdolle
authors.forename: C
authors.initials: C
authors.lastname: Legendre
authors.forename: T
authors.initials: T
authors.lastname: Wroblewski
authors.forename: D
authors.initials: D
authors.lastname: Houssin
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000096:biosynthesis; D001219:Aspartate Aminotransferases / Q000096:biosynthesis; D001794:Blood Pressure; D002648:Child; D002675:Child, Preschool; D005260:Female; D006439:Hemodynamics; D006801:Humans; D008099:Liver / Q000098:blood supply / Q000201:enzymology / Q000503:physiopathology*; D008103:Liver Cirrhosis / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D010101:Oxygen Consumption; D011168:Portal System / Q000502:physiology*; D012039:Regional Blood Flow
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase