title: A very low intake of fat is required to decrease fecal bile acid concentrations in rats.
abstract: The purpose of this study was to determine the effect of different amounts of dietary fat on colonic cell proliferation and fecal bile acid concentrations. Thirty-nine male Sprague-Dawley rats were randomly assigned to three diets (13 rats per diet) containing 5, 10 and 20 g butter/100 g diet. Diets were fed for 3 wk. As fat intake increased, total fecal fat excretion remained constant. When dietary fat was decreased from 20 to 10 g/100 g diet, total fecal bile acid concentrations tended to increase 14.5%. However, a further reduction to 5 g butter/100 g diet significantly decreased fecal total bile acid concentration by 48% from the concentration in feces of rats fed 10 g butter/100 g diet. The concentration of deoxycholate (considered a highly promotive bile acid) was not reduced unless the amount of fat in the diet was reduced to 5 g/100 g. Labeling index was used as an intermediate marker for colon carcinogenesis. For deoxycholate, a decrease of fat intake to 5 g/100 g diet decreased the cecal labeling index relative to those of rats fed 10 or 20 g butter/100 g diet. These data indicate that decreasing the dietary fat from 20 to 10 g/100 g does not decrease fecal bile acid concentration or colonic cell proliferation, but some effects are seen in rats fed 5 g butter/100 g diet.
authors.affiliation: Faculty of Nutrition, Texas A&M University, College Station 77843.
authors.forename: W C
authors.initials: WC
authors.lastname: Chang
authors.forename: J R
authors.initials: JR
authors.lastname: Lupton
authors.forename: W
authors.initials: W
authors.lastname: Frølich
authors.forename: G L
authors.initials: GL
authors.lastname: Schoeffler
authors.forename: M L
authors.initials: ML
authors.lastname: Peterson
authors.forename: X Q
authors.initials: XQ
authors.lastname: Chen
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis*; D002079:Butter; D002455:Cell Division; D003106:Colon / Q000166:cytology*; D003840:Deoxycholic Acid / Q000032:analysis; D004041:Dietary Fats / Q000008:administration & dosage*; D004435:Eating; D002149:Energy Intake; D005243:Feces / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007420:Intestine, Large / Q000033:anatomy & histology / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain
chemical_list: D001647:Bile Acids and Salts; D004041:Dietary Fats; D008055:Lipids; D003840:Deoxycholic Acid; D002079:Butter