Evaluation of the stereoselective metabolism of the chiral analgesic drug etodolac by high-performance liquid chromatography.
The enantiomers of the racemic analgesic drug etodolac have been resolved by fractional crystallization of the diastereomeric salts with optically active 1-phenylethylamine. A high-performance liquid chromatographic method to determine racemic etodolac (assay I) and its major metabolites (assay II) in urine using a conventional reverse-phase column is described. The determination of the enantiomeric ratios of etodolac and the two metabolites 7-hydroxyetodolac and 8-(1'-hydroxyethyl)etodolac was achieved using different protein-bonded chiral stationary phases. The urinary data for five volunteers are presented and show a marked stereoselectivity of the metabolism of etodolac in humans.
Becker-Scharfenkamp
U
U
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Blaschke
G
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003460:Crystallization; D017308:Etodolac / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D013237:Stereoisomerism
D017308:Etodolac