title: 31P NMR studies of rat liver cold preservation with histidine-buffered lactobionate solution.
abstract: The efficiency of a preservation medium, histidine-buffered lactobionate solution (HBLS), was determined by measuring post-ischemic recoveries of ATP and intracellular pH under Krebs-Henseleit buffer (KHB) perfusion. We used NMR spectroscopy to study the effect of 24-h cold ischemia, followed by 4 degrees C then 37 degrees C reperfusion on the isolated rat liver. Three media were compared: University of Wisconsin solution (UW-lactobionate); Bretschneider's solution (HTK); HBLS and HBLS supplemented with 2 mM Gly and 2 mM Cys (HBLSg2) or with 10 mM Gly and 2 mM Cys (HBLSg10). All values were compared to control values measured during pre-ischemic cold perfusion with KHB (ATP = 8.60 +/- 0.6 mumol/g of dry weigh and pH(in) = 7.41 +/- 0.05). The main result from 31P NMR data concerned ATP recovery during cold reperfusion, which was significantly higher in the HBLS group (112 +/- 10%) as compared to the UW and HTK groups (around 66%). The presence of glycine decreased ATP recovery (88 +/- 8% in HBLSg2, 79 +/- 15% in HBLSg10). Higher values of recovered pHin were observed in livers stored in histidine buffered solutions (around 7.30) as compared to UW (around 7.20); histidine was by 13C NMR proved to accumulate in the liver cells, thus ensuring a good buffering capacity. The thermal transition induced a decrease in both ATP level and pHin in all groups. This might be the result of a stimulation of the carbohydrate metabolism (as demonstrated by 13C NMR) especially when glycine was present in the storage solution.
authors.affiliation: Département de Résonance Magnétique, Institut de Biologie Cellulaire du CNRS, Bordeaux, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Delmas-Beauvieux
authors.forename: J L
authors.initials: JL
authors.lastname: Gallis
authors.forename: M
authors.initials: M
authors.lastname: Clerc
authors.forename: P
authors.initials: P
authors.lastname: Canioni
mesh_terms: D000241:Adenosine; D000255:Adenosine Triphosphate / Q000378:metabolism; D000493:Allopurinol; D000818:Animals; D002021:Buffers; D003080:Cold Temperature; D004187:Disaccharides; D005947:Glucose; D005978:Glutathione; D006639:Histidine / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007328:Insulin; D007424:Intracellular Fluid / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008353:Mannitol; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010477:Perfusion; D011189:Potassium Chloride; D011343:Procaine; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions; D014325:Tromethamine
chemical_list: C039984:Bretschneider cardioplegic solution; D002021:Buffers; D004187:Disaccharides; D007328:Insulin; C074097:Krebs-Henseleit solution; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D014325:Tromethamine; D008353:Mannitol; D006639:Histidine; D011343:Procaine; D000493:Allopurinol; C005608:lactobionic acid; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose