title: Isolation and genetic mapping of four microsatellite repeats from chromosome 3p21 using 40 CEPH pedigrees.
abstract: We have isolated and characterized the microsatellite markers D3S1447, D3S1448, D3S1449, and D3S1450, with PIC values of 0.771, 0.644, 0.633, and 0.552, respectively, from a hamster/human chromosome 3 library. By typing 40 CEPH pedigrees with each of these novel markers, we have sublocalized each microsatellite to 3p21 and with multipoint analyses have shown that the likelihood of the order centromere-D3S13-24.24 cM-D3S1450-9.41 cM-D3S1447-7.05 cM-D3F15S2-3 cM-[D3S1448-2.2 cM-D3S1449]-6.13 cM-D3S1100- telomere significantly exceeds any other order (8.0 x 10(3):1). These finely mapped markers will be extremely useful in aiding in the physical characterization of 3p21, which has been implicated in a range of tumor tissues.
authors.affiliation: Department of Genetics, Trinity College, University of Dublin, Ireland.
authors.forename: R
authors.initials: R
authors.lastname: Kumar-Singh
authors.forename: P
authors.initials: P
authors.lastname: Humphries
mesh_terms: D000818:Animals; D001483:Base Sequence; D002503:Centromere; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D006224:Cricetinae; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016615:Telomere
chemical_list: D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers