Construction of a transcription map of a 300 kb region around the human G6PD locus by direct cDNA selection.
A transcription map covering a 300 kb region around the G6PD gene in the human Xq28 region was constructed by the direct cDNA selection method and the analysis of the resulting region-specific enriched cDNA sublibrary. Seven new genes and two loci of endogenous retrovirus HERV-K were identified. The distribution of the genes across the region is strongly non-uniform and follows the non-uniform distribution of GpG islands in the area. While one of the novel genes was found to be highly homologous to bovine smg p25A GDP-dissociation inhibitor, the remaining genes did not detect any homology to known genes. The analysis of region-specific cDNA sublibraries represents a simple, rapid and efficient tool for the generation of a regional transcription map.
Sedlacek
Z
Z
Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Korn
B
B
Konecki
D S
DS
Siebenhaar
R
R
Coy
J F
JF
Kioschis
P
P
Poustka
A
A
D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000032:analysis*; D015226:Dinucleoside Phosphates / Q000032:analysis; D019204:GTP-Binding Proteins / Q000235:genetics; D015723:Gene Library; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014960:X Chromosome; D020692:rab3 GTP-Binding Proteins
D018076:DNA, Complementary; D015226:Dinucleoside Phosphates; D019204:GTP-Binding Proteins; D005954:Glucosephosphate Dehydrogenase; D020692:rab3 GTP-Binding Proteins; C015772:CpG dinucleotide