Cloning and sequencing of the gene encoding spinach ferredoxin-dependent glutamate synthase.
The nucleotide sequences of two cDNA clones, totalling 4948 bp in length, encoding 98% of the 1483 amino acids of the mature form of the ferredoxin-dependent glutamate synthase of spinach chloroplasts have been determined. The amino-terminal sequence of the enzyme has been determined by direct sequencing of the protein. The deduced amino-acid sequence of the spinach enzyme is 83% identical to that of the ferredoxin-dependent maize enzyme and shows significant sequence homology to two prokaryotic NAD(P)H-dependent glutamate synthases. Analysis of spinach genomic DNA indicates the presence of a single-copy gene for the spinach enzyme. Northern analysis reveals the presence of a single 5.5 kb transcript, which is present in higher levels in young spinach leaves than in older leaves.
Nalbantoglu
B
B
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409-1061.
Hirasawa
M
M
Moomaw
C
C
Nguyen
H
H
Knaff
D B
DB
Allen
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D005288:Ferredoxins / Q000494:pharmacology; D005970:Glutamate Synthase / Q000235:genetics*; D008969:Molecular Sequence Data; D014675:Vegetables / Q000201:enzymology
D018076:DNA, Complementary; D005288:Ferredoxins; D005970:Glutamate Synthase