title: Co-expression of four muscarinic receptor genes by the intrinsic neurons of the rat and guinea-pig heart.
abstract: Expression of the messenger RNAs encoding the five different muscarinic acetylcholine receptor subtypes was examined in intracardiac neurons from the rat and guinea-pig heart by in situ hybridization techniques. Newborn guinea-pig intracardiac neurons were studied in dissociated cell culture preparations employing both 35S- and digoxigenin-labelled oligonucleotide probes specific for the m1, m2, m3, m4 or m5 muscarinic receptor messenger RNAs. When 35S-tailed oligonucleotides were used, all intracardiac neurons in culture were found to express m1, m2, m3 and m4, but not m5 messenger RNAs. However after hybridization with digoxigenin-tailed probes, only m1 and m2 transcripts were detected. This may reflect differences in the sensitivity of the two techniques. Further to these experiments, intracardiac ganglia in sections of adult rat heart were studied employing m1-, m2-, m3- or m4-specific, 35S-labelled oligonucleotides, and again, all intracardiac neurons expressed messenger RNA for each of these four muscarinic receptor subtypes. Atrial myocytes in culture were only labelled by [35S]- and digoxigenin-tailed m2 oligonucleotides. No other heart cell type seen expressed messenger RNA for any of the muscarinic receptors. The expression of four different muscarinic receptor transcripts by intrinsic neurons of the heart provides the molecular basis for the diverse muscarinic actions observed in these and other autonomic ganglia.
authors.affiliation: Department of Anatomy and Developmental Biology, University College London, U.K.
authors.forename: C J
authors.initials: CJ
authors.lastname: Hassall
authors.forename: S C
authors.initials: SC
authors.lastname: Stanford
authors.forename: G
authors.initials: G
authors.lastname: Burnstock
authors.forename: N J
authors.initials: NJ
authors.lastname: Buckley
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D005786:Gene Expression Regulation; D005796:Genes; D006168:Guinea Pigs; D006325:Heart Atria / Q000294:innervation; D006346:Heart Septum / Q000294:innervation; D008969:Molecular Sequence Data; D005810:Multigene Family; D009206:Myocardium / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011976:Receptors, Muscarinic / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011976:Receptors, Muscarinic