Uninterrupted translation through putative 12-nucleotide coding gap in sequence of carA: business as usual.
Previous work of others reported an untranslated stretch of 12 nucleotides in the 5' coding sequence of carA from Pseudomonas aeruginosa. However, N-terminal protein sequencing of carA-lacZ translational fusions shows that these 12 nucleotides are normally translated in a continuous triplet manner, both in P. aeruginosa and in Escherichia coli.
Tuohy
T M
TM
Department of Human Genetics, Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112-1107.
Kidd
T
T
Gesteland
R F
RF
Atkins
J F
JF
D000595:Amino Acid Sequence; D001483:Base Sequence; D008025:Ligases / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
D008025:Ligases