Glutathione and glutathione linked enzymes in human small cell lung cancer cell lines.
Glutathione levels and several glutathione-linked enzyme activities have been variably correlated with cisplatin chemosensitivity in cultured neoplastic cells. In order to determine the relative contribution of the glutathione-linked enzymes towards mediating inherent cisplatin resistance in cancer cells, we have measured the chemosensitivity to cisplatin, glutathione levels and activities of glutathione S-transferase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase in 8 cultured human small cell lung cancer (SCLC) cell lines with widely differing cisplatin sensitivities. Of these parameters, only glutathione S-transferase activity correlated with degree of cisplatin resistance in a linear fashion.
Sharma
R
R
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555-1067.
Singhal
S S
SS
Srivastava
S K
SK
Bajpai
K K
KK
Frenkel
E P
EP
Awasthi
S
S
D018288:Carcinoma, Small Cell / Q000201:enzymology*; D002945:Cisplatin / Q000378:metabolism / Q000494:pharmacology*; D004351:Drug Resistance / Q000502:physiology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D008658:Inactivation, Metabolic; D016014:Linear Models; D008175:Lung Neoplasms / Q000201:enzymology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D002945:Cisplatin