Interaction of a novel Tn (GalNAc alpha 1-->Ser/Thr) glycoprotein with Gal, GalNAc and GlcNAc specific lectins.
A naturally occurring Tn glycoprotein (Native ASG-Tn) with GalNAc alpha 1-->Ser/Thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. In a quantitative precipitin assay, this glycoprotein completely precipitated Maclura pomifera (MPA), Vicia villosa B4 (VVL-B4) and Artocarpus integrifolia (Jacalin, AIL). It also reacted well with Helix pomatia (HPL) and Wistaria floribunda (WFL) and precipitated over 75% of the lectin nitrogen added, but poorly with Ricinus communis agglutinin (RCA1), ricin, peanut (Arachis hypogaea, PNA), Abrus precatorius agglutinin (APA) and Triticum vulgaris (WGA). This finding suggests that this novel Tn-glycoprotein may serve as a useful reagent for differentiating Tn and T specific monoclonal antibodies and lectins.
Wu
A M
AM
Glyco-Immunochemistry Research Lab., Chang-Gung Medical College, Kwei-san, Tao-yuan, Taiwan.
Wu
J H
JH
Shen
F
F
D000116:Acetylgalactosamine / Q000032:analysis*; D000117:Acetylglucosamine / Q000032:analysis*; D000818:Animals; D001135:Armadillos; D002240:Carbohydrate Sequence; D005690:Galactose / Q000032:analysis*; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D037102:Lectins; D008969:Molecular Sequence Data; D012694:Serine; D013363:Submandibular Gland / Q000737:chemistry; D013912:Threonine
D006023:Glycoproteins; D037102:Lectins; D013912:Threonine; D012694:Serine; D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D005690:Galactose