Differential localization of A2a adenosine receptor mRNA with D1 and D2 dopamine receptor mRNA in striatal output pathways following a selective lesion of striatonigral neurons.
We have used the suicide transport agent volkensin to produce selective lesions of striatonigral neurons. By in situ hybridization histochemistry unilateral volkensin injections in the substantia nigra decreased the number of D1 receptor mRNA-expressing neurons in the ipsilateral striatum but did not change the number of D2 receptor and A2a adenosine receptor mRNA-expressing neurons. These findings confirm that striatonigral neurons express D1 receptors and suggest that D2-A2a receptor expressing neurons are predominantly localized to other neuronal populations within the striatum.
Pollack
A E
AE
Molecular Neurobiology Laboratory, Massachusetts General Hospital, Boston.
Harrison
M B
MB
Wooten
G F
GF
Fink
J S
JS
D000818:Animals; D001345:Autoradiography; D003342:Corpus Striatum / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D004525:Efferent Pathways / Q000378:metabolism; D006023:Glycoproteins; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008297:Male; D009699:N-Glycosyl Hydrolases; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D037121:Plant Lectins; D010940:Plant Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000235:genetics*; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D018047:Receptors, Purinergic P1 / Q000235:genetics*; D054790:Ribosome Inactivating Proteins, Type 2; D013378:Substantia Nigra / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D014018:Tissue Distribution; D014118:Toxins, Biological / Q000494:pharmacology
D006023:Glycoproteins; D037121:Plant Lectins; D010940:Plant Proteins; D012333:RNA, Messenger; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D018047:Receptors, Purinergic P1; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C049134:volkensin; D009699:N-Glycosyl Hydrolases