Investigations of oligodeoxyinosine for triple helix formation.
The ability of a 17-mer of deoxyinosine to form a triple helix with a (dT)17(dA)17 segment of a 29-mer Watson-Crick duplex was investigated. Under conditions amenable to triple helix formation, (dT)17 showed evidence of complex formation as evidenced by UV melting curves and gel mobility shift assays whereas (dI)17 did not.
Hogeland
J S
JS
Department of Chemistry, Oregon State University, Corvallis 97331.
Weller
D D
DD
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007288:Inosine / Q000031:analogs & derivatives* / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers; D013056:Spectrophotometry, Ultraviolet
D009838:Oligodeoxyribonucleotides; D011108:Polymers; C051802:triplex DNA; D007288:Inosine; D004247:DNA; C012271:deoxyinosine