title: Cloning of the classical guinea pig pancreatic lipase and comparison with the lipase related protein 2.
abstract: Starting from total pancreatic mRNAs, the classical guinea pig pancreatic lipase was cloned using rapid amplification of 3' and 5' cDNA ends. Internal oligonucleotide primers were designed from a partial cDNA clone including the region coding for the lid domain. Using this strategy, we did not amplify the cDNA corresponding to the pancreatic lipase related protein 2 in which the lid domain is deleted. Amino acid sequences of the classical guinea pig pancreatic lipase and the related protein 2 were compared based on the primary and tertiary structures of the classical human pancreatic lipase. Their distinct physiological roles are discussed in the light of functional amino acid differences.
authors.affiliation: Novo Nordisk, Bagsvaerd, Denmark.
authors.forename: F
authors.initials: F
authors.lastname: Carrière
authors.forename: K
authors.initials: K
authors.lastname: Thirstrup
authors.forename: S
authors.initials: S
authors.lastname: Hjorth
authors.forename: E
authors.initials: E
authors.lastname: Boel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006168:Guinea Pigs; D006801:Humans; D008049:Lipase / Q000235:genetics*; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D008049:Lipase; C075988:pancreatic lipase related protein 2