Novel developmentally regulated exon identified in the rat fast skeletal muscle troponin T gene.
In theory, the rat fast skeletal muscle troponin T gene can generate 64 different isoforms. Here we report the identification of a novel alternative exon (exon y) that increases the potential isoform variation to 128. The inclusion of exon y in fast skeletal muscle troponin T mRNA occurs in perinatal, but not adult, skeletal muscle. Exon y is located between exons 8 and 9. This is the first time that a developmentally regulated exon located amongst a set of alternatively spliced exons has been described. Exon y is included in two mRNA isoforms. The proteins that these mRNAs would encode have molecular masses greater than that of the largest fast skeletal muscle troponin T isoform lacking exon y. These two proteins correlate well in both size and pattern of expression with the two fast skeletal muscle troponin T isoforms expressed in perinatal skeletal muscle. These results indicate that there is coordinated regulation of the splicing of exon y with other alternative exons.
Morgan
M J
MJ
Department of Basic Sciences, Royal Veterinary College, University of London, UK.
Earnshaw
J C
JC
Dhoot
G K
GK
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA; D005091:Exons; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014336:Troponin / Q000096:biosynthesis / Q000235:genetics*; D020107:Troponin T
D014336:Troponin; D020107:Troponin T; D004247:DNA