Radioimmunoassay of glutathione peroxidase in human serum.
Glutathione peroxidase (GSHPx) was purified from human serum and used for immunization of rabbits. Antiserum bound up to 75% of added 125I-GSHPx after precipitation with a second antibody. Human serum, but not sera from eight animal species, inhibited the binding of labelled GSHPx, indicating that the antiserum did not react with GSHPx from these species. GSHPx could be measured in less than 10 microliters of human serum by radioimmunoassay. In sera with widely varying selenium concentrations (0.1-2.9 mumol/l) the amount of GSHPx protein (0.3-6.3 mg/l) was strongly correlated with GSHPx activity (r = 0.94) and it was also correlated with serum selenium concentrations (r = 0.64). This indicates that GSHPx protein may be a valuable biological marker of selenium status. In samples with serum selenium concentrations of 0.8-1.2 mumol/l, the concentration of GSHPx was 3.3 (0.4) mg/l (mean (S.D.)), or 0.04 (0.005) mumol/l. This corresponded to 0.16 (0.02) mumol/l of GSHPx selenium and 16% (2.8)% of total serum selenium. The data suggest that the method can be used to measure the proportion of serum selenium that is located in GSHPx. Following storage of serum at room temperature, both serum GSHPx protein and activity declined, but addition of glutathione protected both GSHPx protein and activity.
Huang
W
W
Department of Applied Nutrition and Food Chemistry, Chemical Center, University of Lund, Sweden.
Akesson
B
B
D000818:Animals; D002417:Cattle; D005979:Glutathione Peroxidase / Q000097:blood* / Q000276:immunology; D006041:Goats; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D007700:Kinetics; D051379:Mice; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D012643:Selenium / Q000097:blood; D013045:Species Specificity; D013552:Swine
D005979:Glutathione Peroxidase; D012643:Selenium