Hormonal modulation of the cutaneous initiation of lordosis in infant and adult rats.
The purpose of these experiments is to compare the regional specificity (Experiment 1) and the hormonal modulation (Experiment 2) of the cutaneous initiation of lordosis in 4- to 6-day-old male and female rats (infants) and in 60- to 90-day-old female rats (adults). In Experiment 1, subjects were primed with 100 micrograms estradiol benzoate (EB) and 0.5 mg progesterone (P) and were denervated on the Waist (dermatomes L1-L3), Midriff (dermatomes T10-L3), Flanks (dermatomes L4-L6), or Sides (dermatomes T10-L6). In infants, there were no significant differences between males and females. Denervation of the Waist, Midriff, or Sides but not of the Flanks significantly decreased the percentage of subjects displaying lordosis, lordosis quotient (LQ), and mean lordosis duration; no significant differences were obtained among Waist-, Midriff, or Sides-denervated infants. In contrast, denervation of the Sides but not of the Waist significantly decreased LQ and mean lordosis intensity among adults. In Experiment 2, Waist-denervated infants and their surgical Controls were treated either with 100 micrograms EB and 0.5 mg P or with the oil vehicle; Waist-denervated adults and their surgical Controls received either 100 or 10 micrograms EB (no P). Regardless of hormone treatment, denervation of the Waist significantly decreased LQ and lordosis duration in infants and decreased LQ and lordosis intensity in adults. In infants, the only effect of priming with EB and P was to increase the percentage of pups showing lordosis and lordosis duration among the surgical Controls. In contrast, priming with 100 micrograms EB significantly increased the percentage of rats displaying lordosis, LQ, and lordosis intensity among Waist-denervated adults. These data suggest that cutaneous input from the Waist is important for eliciting lordosis in both infant and adult rats, and that the importance of this input is modulated by hormone priming in adult but not infant rats.
Benedict
G S
GS
Columbia University, New York, New York 10027.
Williams
C L
CL
D000818:Animals; D003714:Denervation; D004958:Estradiol / Q000502:physiology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000502:physiology*; D008297:Male; D008465:Mechanoreceptors / Q000502:physiology*; D010525:Peripheral Nerves / Q000502:physiology; D011374:Progesterone / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012733:Sex Differentiation / Q000502:physiology; D012726:Sexual Behavior, Animal / Q000502:physiology*; D012741:Sexual Maturation / Q000502:physiology*; D012867:Skin / Q000294:innervation*
D012739:Gonadal Steroid Hormones; D011374:Progesterone; D004958:Estradiol