title: Glutathione conjugation with the pyrrolizidine alkaloid, jacobine.
abstract: The reaction of glutathione with the epoxide containing pyrrolizidine alkaloid, jacobine, was catalyzed by guinea pig hepatic glutathione-S-transferase enzymes in in vitro experiments; the rate of the reaction in the presence of rat hepatic glutathione-S-transferases did not differ from the non-enzymatic rate. Using ion-pairing liquid chromatography we were able to isolate the conjugate and obtain a daughter ion spectrum using Fast Atom Bombardment Mass Spectrometry together with Collisionally Activated Dissociation/Mass Analyzed Kinetic Energy experiments.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, University of California, Davis 95616.
authors.forename: S R
authors.initials: SR
authors.lastname: Dueker
authors.forename: M W
authors.initials: MW
authors.lastname: Lamé
authors.forename: A D
authors.initials: AD
authors.lastname: Jones
authors.forename: D
authors.initials: D
authors.lastname: Morin
authors.forename: H J
authors.initials: HJ
authors.lastname: Segall
mesh_terms: D000818:Animals; D003600:Cytosol / Q000201:enzymology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D006168:Guinea Pigs; D008658:Inactivation, Metabolic; D008099:Liver / Q000201:enzymology*; D008297:Male; D011763:Pyrrolizidine Alkaloids / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D011763:Pyrrolizidine Alkaloids; C085554:glutathione-jacobine conjugate; C013581:jacobine; D005982:Glutathione Transferase; D005978:Glutathione