cis-acting DNA elements involved in oocyte-specific expression of mouse sperm receptor gene mZP3 are located close to the gene's transcription start site.
We report that cis-acting DNA elements involved in oocyte-specific expression of the mouse sperm receptor gene (mZP3) are located close to the gene's transcription start site. Mice bearing a transgene that consists of only 153 nt of mZP3 5'-flanking region fused to the firefly luciferase gene (153-ZP3/LUC) expressed the reporter gene in ovary not in a wide variety of tissues; although two of three lines carrying 153-ZP3/LUC also expressed the transgene in forebrain and hypothalamus. Within the ovaries of transgenic mice, luciferase activity was restricted to growing oocytes. However, levels of luciferase activity in these oocytes were lower than those in oocytes from mice bearing transgenes that contain a larger segment of mZP3 5'-flanking region (470-6,500 nt) fused to the firefly luciferase gene. Mice bearing a transgene that consists of 470 nt of mZP3 5'-flanking region and mZP3 intragenic sequences (ZDT) were also analyzed. The presence of mZP3 intragenic sequences did not result in significantly increased levels of firefly luciferase activity in oocytes of mice carrying the ZDT transgene. Overall, these results suggest that as little as 153 nt of mZP3 5'-flanking region is sufficient to target expression of the firefly luciferase gene to mouse oocytes and that the mZP3 intragenic sequences probably do not contain enhancer elements. Rather, enhancer elements are probably present between-153 and -470 nt of the mZP3 5'-flanking region.
Lira
S A
SA
Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Schickler
M
M
Wassarman
P M
PM
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D008156:Luciferases / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000235:genetics*; D013084:Sperm-Ovum Interactions / Q000235:genetics*; D014158:Transcription, Genetic
D011956:Receptors, Cell Surface; C035027:egg surface sperm receptor; D004247:DNA; D008156:Luciferases