Continuous electrophoretic separations in narrow channels coupled to small-bore capillaries.
Continuous zone electrophoretic separations in channels have been demonstrated. This new technique has the potential to continuously sample and separate analytes from volume-limited microenvironments. A small-bore capillary is used to electrophoretically sample, but not separate, a mixture of dansylated amino acids. The capillary is coupled to a quartz channel structure in a manner which allows continuous injection of the sampled material into the channel. The channel functions to continuously separate the sampled material via electrophoresis. A laser-induced fluorescence detection scheme, which involves two fiber optic arrays situated at the channel exit, monitors eluting analytes. A continuous separation of dansylated amino acids on the time scale of a few minutes demonstrates the utility of the technique. Sampling has been performed continuously up to 400 s, and initial detection limits are approximately 30 microM.
Mesaros
J M
JM
Department of Chemistry, Pennsylvania State University, University Park 16802.
Luo
G
G
Roeraade
J
J
Ewing
A G
AG
D003619:Dansyl Compounds / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D007834:Lasers; D008239:Lysine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence
D003619:Dansyl Compounds; C036851:dansyllysine; D008239:Lysine