Melatonin receptors in purified cell nuclei of liver.
Specific binding of [125I]iodomelatonin to homogenates from purified rat liver nuclei was characterized. The binding is rapid, reversible, saturable and of high affinity. Specific binding seems to be found in the nuclear protein fraction, since after precipitation of the proteins with trichloroacetic acid, the specific binding disappeared. The Kd (180 +/- 20 pM) and Bmax (9.1 +/- 0.03 fmol/mg protein) values (mean +/- S.E.M.) agree with the melatonin concentration in nuclei and may imply a physiological locus for melatonin action.
Acuña-Castroviejo
D
D
Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio 78284.
Pablos
M I
MI
Menendez-Pelaez
A
A
Reiter
R J
RJ
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008550:Melatonin / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism*; D044094:Receptors, Melatonin
D011956:Receptors, Cell Surface; D044094:Receptors, Melatonin; D008550:Melatonin