title: Isolation and complete sequence of CBR, a gene encoding a putative cytochrome b reductase in Saccharomyces cerevisiae.
abstract: We have isolated and characterised a novel yeast gene, CBR (cytochrome b reductase), encoding a 35-kDa yeast novobiocin-binding protein. The predicted protein sequence of CBR displays considerable similarity to both plant nitrate reductases and mammalian cytochrome b5 reductases indicating that it is a putative member of the flavoprotein pyridine-nucleotide-cytochrome-reductase family. Disruption of CBR is not lethal under various growth conditions, suggesting the presence of some functional overlap with other reductases, possibly with the cytochrome P-450 reductase.
authors.affiliation: Department of Genetics, University of Leicester, England.
authors.forename: M
authors.initials: M
authors.lastname: Csukai
authors.forename: M
authors.initials: M
authors.lastname: Murray
authors.forename: E
authors.initials: E
authors.lastname: Orr
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D002846:Chromatography, Affinity; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D003579:Cytochrome Reductases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D042966:Cytochrome-B(5) Reductase; D004271:DNA, Fungal / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli; D015723:Gene Library; D005800:Genes, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D009565:Nitrate Reductases / Q000235:genetics; D009675:Novobiocin / Q000378:metabolism; D016366:Open Reading Frames; D010944:Plants / Q000201:enzymology; D012331:RNA, Fungal / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D004271:DNA, Fungal; D012331:RNA, Fungal; D009675:Novobiocin; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase; D009565:Nitrate Reductases