[Expression of the gag-precursor of the human T-cell leukemia virus type I in Escherichia coli: study of the stability and antigenic properties of virus-specific hybrid proteins].
A set of recombinant plasmids containing sequences of HTLV-I viral gag-gene has been constructed on the basis of pUR290-pUR292 vector plasmids. The resulting hybrid proteins containing different fragments of GAG-precursor in the C-end of beta-galactosidase differed to a large extent in stability in Escherichia coli cells. The presence of an N-end fragment of GAG-precursor in the recombinants decreases drastically their resistance to bacterial proteases. Elimination of the fragment resulted in obtaining the recombinant plasmid pGdN coding for high rate synthesis (up to 30% of total cellular protein) of gag-specific hybrid polypeptide in Escherichia coli HB101 cells. This 145 kDa protein efficiently interacts with HTLV-I positive sera. It can be used in diagnostic test-systems for indicating HTLV-I infected persons.
Sankov
M N
MN
Bobkov
A F
AF
Garaev
M M
MM
D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015683:Gene Products, gag / Q000235:genetics* / Q000276:immunology; D015750:Genes, gag; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics* / Q000276:immunology; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; D054301:gag Gene Products, Human Immunodeficiency Virus; C060955:p19 protein, Human T-lymphotropic virus 1
Ekspressiia gag-predshestvennika virusa T-kletochnogo leĭkoza cheloveka tipa I v kletkakh Escherichia coli: izuchenie stabil'nosti i antigennykh svoĭstv virusspetsificheskikh gibridnykh belkov.