Removal of Rab GTP-binding proteins from Golgi membranes by GDP dissociation inhibitor inhibits inter-cisternal transport in the Golgi stacks.
Rab proteins are a family of Ras-like GTPases involved in intracellular membrane traffic. Rab GDI, a cytosolic protein which inhibits the dissociation of GDP from various Rab proteins, is required to maintain a pool of Rab proteins in the cytosol. We describe the purification of a cytosolic factor from bovine liver that inhibits intra-cisternal transport between the Golgi stacks. We identify this factor as a Rab GDI. Half-maximal inhibition of transport was observed in the presence of the same concentration of GDI that is required for removal of Rab proteins from the Golgi.
Elazar
Z
Z
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Mayer
T
T
Rothman
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002417:Cattle; D002474:Cell-Free System; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D020730:Guanine Nucleotide Dissociation Inhibitors; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020692:rab3 GTP-Binding Proteins; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
D020730:Guanine Nucleotide Dissociation Inhibitors; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D019204:GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins