title: Genes encoding antigenic surface glycoproteins in Pneumocystis from humans.
abstract: Pneumocystis is a eukaryotic microbe that causes pneumocystosis, an AIDS-associated pneumonia. Pneumocystosis also occurs in many other mammalian species, and animal-derived organisms have been extensively utilized in Pneumocystis research. Pneumocystis from diverse hosts contain a large glycoprotein (gpA/MSG) on the surface. Antibodies elicited against gpA/MSG of Pneumocystis from humans sometimes cross-react with epitopes on proteins of similar size from Pneumocystis from other host species. Here we report the isolation and partial sequence of two presumptive gpA/MSG genes from human-derived Pneumocystis. The cloned human-derived Pneumocystis gpA/MSG genes and predicted peptides were different from those previously isolated from Pneumocystis from rats and ferrets. The genome of human-derived Pneumocystis contained multiple copies of sequences related to the two cloned gpA/MSG genes.
authors.affiliation: Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, College of Medicine, Ohio 45267.
authors.forename: S L
authors.initials: SL
authors.lastname: Stringer
authors.forename: T
authors.initials: T
authors.lastname: Garbe
authors.forename: S M
authors.initials: SM
authors.lastname: Sunkin
authors.forename: J R
authors.initials: JR
authors.lastname: Stringer
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000595:Amino Acid Sequence; D000818:Animals; D000946:Antigens, Fungal / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis; D005800:Genes, Fungal; D006801:Humans; D008168:Lung / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016720:Pneumocystis Infections / Q000209:etiology / Q000382:microbiology; D051381:Rats; D011914:Rats, Inbred BN; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000946:Antigens, Fungal; D004271:DNA, Fungal; D008562:Membrane Glycoproteins