title: Vascular permeability enhancing activity of Porphyromonas gingivalis protease in guinea pigs.
abstract: Porphyromonas gingivalis protease, which had been isolated from a culture supernatant, caused vascular permeability enhancement in a dose-dependent manner when injected into guinea pig skin. The permeability-enhancing reaction caused by the protease was not affected by treatment with antihistamine, but was greatly augmented by simultaneous injection of a kinin potentiator, carboxypeptidase N inhibitor. However, the reaction was inhibited by soybean trypsin inhibitor or alpha 2-antiplasmin, although both of these inhibitors could not inhibit P. gingivalis protease at all by themselves. A bradykinin-degrading enzyme, carboxypeptidase B, weakened this vascular reaction. Results described indicate that the permeability-enhancing reaction induced by the protease is caused by activation, of the kallikrein-kinin cascade in the tissue.
authors.affiliation: Department of Oral Microbiology, Fukuoka Dental College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kaminishi
authors.forename: T
authors.initials: T
authors.lastname: Cho
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: A
authors.initials: A
authors.lastname: Iwata
authors.forename: K
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Hagihara
authors.forename: H
authors.initials: H
authors.lastname: Maeda
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D002847:Chromatography, Agarose; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D016234:Kallikrein-Kinin System / Q000187:drug effects; D007610:Kallikreins / Q000378:metabolism; D008297:Male; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology; D012867:Skin / Q000098:blood supply; D013379:Substrate Specificity
chemical_list: D011480:Protease Inhibitors; D010447:Peptide Hydrolases; D007610:Kallikreins