Atrial natriuretic peptides in the heart and hemolymph of the oyster, Crassostrea virginica: a comparison with vertebrates.
1. The content of atrial natriuretic peptides (ANPs) in the auricles of oysters, Crassostrea virginica, was significantly (P < 0.01) greater than in their ventricles. 2. High-performance gel permeation chromatography (HP-GPC) followed by ANF radioimmunoassay revealed two peaks in both oyster and vertebrate (rat) hearts--a major peak where the 12.6-14 kDa ANF prohormone elutes and a smaller peak where the pure human form of ANF elutes. 3. HP-GPC evaluation followed by proANF 31-67 radioimmunoassay revealed only an ANF-like prohormone while HP-GPC followed by proANF 1-30 radioimmunoassay revealed the ANF prohormone and a proANF 1-30-like peptide in oyster and rat hearts. 4. ANPs concentrations in hemolymph were 940 +/- 129, 225 +/- 25, and 100 +/- 10 pg/ml by the proANF 1-30, proANF 31-67, and ANF radioimmunoassays, respectively. 5. Atrial natriuretic-like peptides are present in the oyster heart in molecular species similar to vertebrate species and these peptides are also present in hemolymph.
Vesely
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Department of Biology, University of South Florida, Tampa.
Gower
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WR
Giordano
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AT
Friedl
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D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D002850:Chromatography, Gel; D000431:Ethanol; D006458:Hemolymph / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D009206:Myocardium / Q000737:chemistry*; D009318:Natriuresis; D010125:Ostreidae / Q000737:chemistry*; D011498:Protein Precursors; D011863:Radioimmunoassay; D051381:Rats; D012680:Sensitivity and Specificity; D014714:Vertebrates / Q000378:metabolism*
D011498:Protein Precursors; D000431:Ethanol; D009320:Atrial Natriuretic Factor