Dicistronic selection for nuclear proteins in living animal cells.
We present an expression/selection system designed for the purification of cell lines inducibly expressing genes coding for unselectable proteins by using dicistronic selection for the cell surface marker CD4. This system enabled us to establish and purify c-myb expressing variants of the v-myb transformed chicken monoblast cell line BM2 with high efficiency.
Smarda
J
J
Department of Microbiology, SUNY at Stony Brook 11794-5222.
Lipsick
J S
JS
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis*; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular / Q000379:methods*; D004574:Electromagnetic Fields; D009857:Oncogenes; D010957:Plasmids; D011994:Recombinant Proteins / Q000032:analysis*
D015704:CD4 Antigens; D011994:Recombinant Proteins