title: Primary structure of the murine monoclonal IgG2a antibody mAb735 against alpha (2-8) polysialic acid. 2. Amino acid sequence of the heavy (H-) chain Fd' region.
abstract: The complete amino acid sequence of the Fd' region including the VH part, the CH1 domain, and the hinge segment of the biologically relevant monoclonal mouse anti-alpha (2-8) polysialic acid antibody mAb735 is presented. The reduced and carboxymethylated H-chain was digested with trypsin and cyanogen bromide. For subfragmentation selected peptides were cleaved with thermolysin and endoproteinase Asp-N. The generated peptides were isolated by RP-HPLC and characterized by sequence analysis, plasma desorption mass spectrometry (PDMS), and amino acid analysis. The N-terminal sequence was determined after enzymatic deprotection with pyroglutamate aminopeptidase. According to Kabat et al. the variable region of the H-chain belongs to the subgroup II. Sequence data from the constant region indicate that mAb735 represents the gamma 2a isotype.
authors.affiliation: Max-Planck-Institut für experimentelle Medizin, Abteilung Immunchemie, Göttingen.
authors.forename: S
authors.initials: S
authors.lastname: Klebert
authors.forename: H D
authors.initials: HD
authors.lastname: Kratzin
authors.forename: B
authors.initials: B
authors.lastname: Zimmermann
authors.forename: M
authors.initials: M
authors.lastname: Vaesen
authors.forename: M
authors.initials: M
authors.lastname: Frosch
authors.forename: C
authors.initials: C
authors.lastname: Weisgerber
authors.forename: D
authors.initials: D
authors.lastname: Bitter-Suermann
authors.forename: N
authors.initials: N
authors.lastname: Hilschmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D006825:Hybridomas; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry*; D013058:Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012794:Sialic Acids / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D012794:Sialic Acids; C071290:alpha(2-8)polysialic acid; C100277:immunoglobulins, Fd