Comparison of the restriction patterns of equine herpesvirus (EHV-1) strains isolated for eight years in France.
The equine herpes viruses strains (EHV) isolated from organs of aborted foetuses or from nasal swabs have been analysed by comparison of their restriction endonucleases patterns using two enzymes, Bam HI and Pst I. The majority of the clinical samples came from the west part of France ("Normandie") after abortions or respiratory disorders. All the viruses isolated were EHV-1 strains whose patterns show considerable homogeneity although some differences can be described. The genomic DNAs of the same twenty strains have been digested by the Pst I enzyme, which induced a great number of restriction fragments. It allows a more precise epidemiological study between strains isolated in the same studs with different Bam HI patterns or between strains with identical Bam HI profiles but with distinct respiratory or abortigenic pathogenicity. No strain isolated from aborted foetuses or nasal swabs presented the vaccinal pattern.
Zientara
S
S
CNEVA-Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort Cedex, France.
Plateau
E
E
Sailleau
C
C
Crucière
C
C
D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005602:France; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000145:classification* / Q000235:genetics; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D009297:Nasal Mucosa / Q000382:microbiology; D011247:Pregnancy; D015183:Restriction Mapping
D004279:DNA, Viral