Evidence that the F2-isoprostane, 8-epi-prostaglandin F2 alpha, is formed in vivo.
F2-isoprostanes are prostaglandin (PG)F2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F2-isoprostane whose formation should be favored is 8-epi-PGF2 alpha. 8-Epi-PGF2 alpha has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF2 alpha is, in fact, formed in vivo by demonstrating that an endogenous F2-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF2 alpha co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF2 alpha.
Morrow
J D
JD
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232.
Minton
T A
TA
Badr
K F
KF
Roberts
L J
LJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014020:Tissue Extracts / Q000737:chemistry
D014020:Tissue Extracts; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost