title: Rapid glutamate decarboxylase assay for detection of Escherichia coli.
abstract: A rapid test procedure for the enzyme glutamate decarboxylase was developed for detection of Escherichia coli. The assay procedure was able to confirm the presence of E. coli in enteric broth cultures with 95% specificity for both pure cultures and environmental samples. The procedure was capable of detecting survivors among chlorine-exposed cells.
authors.affiliation: Drinking Water Research Division, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
authors.forename: E W
authors.initials: EW
authors.lastname: Rice
authors.forename: C H
authors.initials: CH
authors.lastname: Johnson
authors.forename: M E
authors.initials: ME
authors.lastname: Dunnigan
authors.forename: D J
authors.initials: DJ
authors.lastname: Reasoner
mesh_terms: D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification*; D005968:Glutamate Decarboxylase / Q000032:analysis*; D012680:Sensitivity and Specificity; D014871:Water Microbiology
chemical_list: D005968:Glutamate Decarboxylase