TGF-beta 1 inhibits polyamine biosynthesis in K 562 leukemic cells.
The present study proved that TGF-beta 1 significantly inhibited the growth of K 562 cells. The drop in cell numbers after 24 h incubation with increasing concentrations of TGF-beta 1 (0.01, 0.1, 1.0, 10.0 ng/ml) was accompanied by significant suppression of the activity of two key enzymes of polyamine biosynthesis: ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC). In contrast to ODC and SAMDC activity, TGF-beta 1 did not significantly affect the absolute concentration of spermidine and spermine in K 562 cells. We suppose that the lack of an evident drop in concentration of spermidine and spermine in spite of a significant decrease in ODC and SAMDC activity in K 562 cells exposed to TGF-beta 1 resulted from the uptake of polyamines from the extracellular space.
Motyl
T
T
Department of Animal Physiology, Warsaw Agricultural University, Poland.
Kasterka
M
M
Grzelkowska
K
K
Blachowski
S
S
Sysa
P
P
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D000518:Eflornithine / Q000494:pharmacology; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008935:Mitoguazone / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology; D011073:Polyamines / Q000378:metabolism*; D047428:Protein Kinase Inhibitors; D011700:Putrescine / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D007546:Isoquinolines; D010879:Piperazines; D011073:Polyamines; D047428:Protein Kinase Inhibitors; D016212:Transforming Growth Factor beta; D013096:Spermine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; D008935:Mitoguazone; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine