Hippocampal serotonin levels influence the expression of S100 beta detected by immunocytochemistry.
The response of astrocytes to pharmacological alterations of the serotonin (5-HT) content of the dorsal area dentata was studied by immunocytochemistry for S100 beta. Stereological analysis revealed changes in the density of astrocytic elements in conditions of 5-HT depletion and elevated 5-HT levels. There was a direct relationship between the expression of S100 beta and the level of 5-HT in the hippocampal formation. Thus when 5-HT was reduced by PCPA treatment, S100 beta immunoreactivity was also reduced. The converse of these observations was obtained in cases where the 5-HT levels were increased by fluoxetine administration. These immunocytochemical data are consistent with previous reports of the response of astrocytes to 5-HT in vitro.
Haring
J H
JH
Department of Anatomy and Neurobiology, St. Louis University Medical Center, MO 63104.
Hagan
A
A
Olson
J
J
Rodgers
B
B
D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000648:ultrastructure; D010134:Fenclonine / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009414:Nerve Growth Factors; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D010133:p-Chloroamphetamine / Q000494:pharmacology
D005904:Glial Fibrillary Acidic Protein; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D012701:Serotonin; D010133:p-Chloroamphetamine; D010134:Fenclonine