The exon-intron structure and chromosomal localization of the mouse macrophage mannose receptor gene Mrc1: identification of a Ricin-like domain at the N-terminus of the receptor.
The macrophage mannose receptor is a transmembrane protein that is expressed on the surface of mature macrophages. The ectodomain of the receptor contains multiple domains, eight of which belong to the calcium-dependent C-type lectin family. The mannose receptor binds to carbohydrate polymers that have a high content of mannose. This property allows this protein to function as a phagocytic receptor that participates in first-line host defense against invading microorganisms. In this paper we describe the intron-exon structure of the mouse macrophage mannose receptor gene which was found to span at least 70 kilobases. We also report the localization of this gene, termed Mrc1, to mouse Chromosome 2. Like its human counterpart, Mrc1 contains 30 exons and 29 introns. A protein module that resembles a subdomain of the B chain of the plant lectin Ricin has been found within the N-terminal cysteine-rich domain of the mannose receptor.
Harris
N
N
Division of Hematology/Oncology, Children's Hospital, Boston, MA.
Peters
L L
LL
Eicher
E M
EM
Rits
M
M
Raspberry
D
D
Eichbaum
Q G
QG
Super
M
M
Ezekowitz
R A
RA
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D005091:Exons / Q000235:genetics*; D005260:Female; D007438:Introns / Q000235:genetics*; D037181:Lectins, C-Type; D008264:Macrophages / Q000737:chemistry*; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009115:Muridae; D011956:Receptors, Cell Surface / Q000235:genetics*; D012276:Ricin / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D012276:Ricin