Regional intraarterial infusion of methotrexate with leucovorin rescue in a rat tumor model.
Walker 2S6 carcinosarcoma was transplanted in the paw of 52 male Wistar rats. A week later, the animals were divided into 6 separate groups and treated in different ways, as follows. Group I (control): no treatment. Group II: femoral artery ligation (FAL). Group III: ligation-intraarterial chemotherapy (LIC) with methotrexate (MTX). Group IV: the same treatment followed by leucovorin rescue (LIC+RES). Group V: tourniquet intraarterial chemotherapy and rescue (TIC+RES). Group VI: the same treatment applied via the iliac artery (iliac TIC+RES). The best results (primary tumor eradication: 8/10 cases, propagation of the tumor to the inguinal region: 4/10 cases, necroses of the distal part of the paw: 0/10 cases, p > 0.05) were observed in group VI, where iliac LIC+RES was performed. Analysis of the treatment's mechanism of action revealed that there is an area of optimal treatment in the middle of the limb, and two peripheral zones of under-treatment. At the proximal zone (inguinal region) propagation of the tumor can occur, whereas at the distal zone necrosis of the paw can be manifested.
Pologeorgis
G
G
Department of Surgery, Hellenic Anticancer Institute, Saint Savvas Hospital, Athens, Greece.
Agnantis
N
N
Kottaridis
S
S
D000818:Animals; D002279:Carcinoma 256, Walker / Q000188:drug therapy* / Q000473:pathology; D007261:Infusions, Intra-Arterial; D002955:Leucovorin / Q000008:administration & dosage / Q000627:therapeutic use*; D008207:Lymphatic Metastasis / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage / Q000627:therapeutic use*; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar
D002955:Leucovorin; D008727:Methotrexate