Optical resolution by high-performance capillary electrophoresis. Micellar electrokinetic chromatography with sodium N-dodecanoyl-L-glutamate and digitonin.
Optical resolution by micellar electrokinetic chromatography with sodium N-dodecanoyl-L-glutamate (SDGlu) and with digitonin-sodium taurodeoxycholate (STDC) mixed micelles was investigated. Addition of sodium dodecyl sulphate, urea and methanol to SDGlu micellar solutions could give improved peak shapes and resolution. With SDGlu, phenylthiohydantoin (PTH) derivatives of five DL-amino acids (alpha-aminobutyric acid, methionine, norvaline, tryptophan and norleucine) were separated from each other and each pair of enantiomers was optically resolved. On the other hand, three PTH-DL-amino acids (norvaline, valine and alpha-aminobutyric acid) were also successfully resolved with a digitonin-STDC-urea solution (pH 2.5).
Otsuka
K
K
Department of Industrial Chemistry, Osaka Prefectural College of Technology, Japan.
Kashihara
M
M
Kawaguchi
Y
Y
Koike
R
R
Hisamitsu
T
T
Terabe
S
S
D000596:Amino Acids / Q000302:isolation & purification*; D004072:Digitonin; D004560:Electricity; D004586:Electrophoresis / Q000379:methods*; D005971:Glutamates; D008823:Micelles; D010669:Phenylthiohydantoin; D013237:Stereoisomerism
D000596:Amino Acids; D005971:Glutamates; D008823:Micelles; D010669:Phenylthiohydantoin; D004072:Digitonin; C085318:N-dodecanoylglutamic acid