Cholesterol peroxidation potential as influenced by dietary fat type.
Oxidation of lipoproteins is believed to play a key role in atherogenesis. In this study, influence of two fat vehicles, corn oil (unsaturated) or beef tallow (saturated), on cholesterol induced effects in terms of a) plasma lipoprotein peroxidation and b) aortic accumulation of cholesterol, cholesterol oxide and malonaldehyde was investigated in weanling rabbits. Although the increase in plasma cholesterol induced by cholesterol feeding was similar in both the groups Cu2+ ion induced peroxidation of lipoprotein cholesterol to oxysterols was significantly higher in corn oil group. This difference persisted even at 5 weeks after switching to chow diet. Aortic content of cholesterol and cholesterol oxides was also significantly higher in corn oil group at the end of chow diet period. Aortic accumulation of malonaldehyde (detected by immunocytochemistry) appeared to be greater in corn oil group at the end of the study. These results suggest that fatty acid composition of a given diet during exposure to high dietary cholesterol can influence cholesterol peroxidation to oxysterols and its accumulation in aorta.
Bhadra
S
S
Department of Internal Medicine, University of Cincinnati Medical Center, OH 45267.
Banavali
S D
SD
Agrawal
M
M
Subbiah
M T
MT
D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000166:cytology; D001835:Body Weight; D002784:Cholesterol / Q000097:blood* / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D003314:Corn Oil / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D005223:Fats / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D011817:Rabbits
D004041:Dietary Fats; D005223:Fats; D008315:Malondialdehyde; D003300:Copper; D003314:Corn Oil; D002784:Cholesterol; C013698:tallow