[Kinetic parameters of GABA receptor binding by rat adenohypophyseal membranes under conditions of modelling various corticosteroid and ACTH levels in the body].
Kinetic parameters of 14C-GABA specific binding by rat adenohypophyseal plasma membranes were studied in experiments on modelling various corticosteroid and ACTH levels in animal body. A single hydrocortisone injection did not change K alpha for high- and low-affinity GABA receptors, the number of the former (Bmax) increasing in this case. Repeated hydrocortisone injections were associated with K alpha reduction for high-affinity GABA receptors and a noticeable increase of K alpha for low-affinity receptors, with their number reducing. ACTH injection did not change the kinetic parameters of GABA binding with receptors. The number of high-affinity GABA receptors and their affinity reduced after removal of adrenals whereas the number of low-affinity receptors in this case was increasing. A single hydrocortisone injection to adrenalectomized rats had a normalizing effect on adenohypophyseal GABA receptors. Analysis of the results and changes in blood hormonal levels indicated that affinity changes in high-affinity receptors and changed number of low-affinity adenohypophyseal GABA receptors correlated with changes in ACTH and hydrocortisone changes.
Mishunina
T M
TM
Kononenko
V Ia
VIa
D000311:Adrenal Glands / Q000502:physiology; D000324:Adrenocorticotropic Hormone / Q000097:blood*; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D003345:Corticosterone / Q000097:blood*; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018079:Receptors, GABA / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D018079:Receptors, GABA; D005680:gamma-Aminobutyric Acid; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone
Kineticheskie parametry retseptornogo sviazyvaniia GAMK membranami adenogipofiza krys v usloviiakh modelirovaniia razlichnykh urovneĭ kortikosteroidov i AKTG v organizme.