Preservation time dependent morphological changes in cold stored human donor pancreas.
Pancreatic transplantation is being used to treat insulin-dependent diabetes. An intact structure of the graft is a prerequisite for preserved function and we therefore monitored the light microscopic and ultrastructural changes in 30 human donor pancreases stored in the cold in University of Wisconsin preservation solution. Twenty-three pancreases were stored for less than 24 h and 7 for more than 30 h. All glands stored longer than 30 h displayed cytoplasmic vacuolisation in a variable proportion of acinar cells. In addition, the glands stored over 40 h showed focal acinar necrosis. Endocrine tissue was only slightly affected, while duct cells showed no changes. It is concluded that cold preserved pancreases stored for less than 24 h are best for transplantation purposes and that acinar cells are more sensitive to ischaemic damage than endocrine and duct cells.
In't Veld
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Department of Pathology, Free University of Brussels VUB, Belgium.
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Klöppel
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D000241:Adenosine; D000293:Adolescent; D000328:Adult; D000493:Allopurinol; D002648:Child; D015925:Cryopreservation; D005260:Female; D005978:Glutathione; D006801:Humans; D007328:Insulin; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D019188:Organ Preservation Solutions; D010179:Pancreas / Q000033:anatomy & histology* / Q000648:ultrastructure; D011887:Raffinose; D013997:Time Factors; D014617:Vacuoles / Q000648:ultrastructure
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose