title: Spontaneous host endothelial growth on bioprosthetic valves and its relation to calcification.
abstract: We studied host endothelial growth and calcification of bovine pericardial valve prostheses treated with: (A) 0.625% glutaraldehyde + 4% formaldehyde, (B) 99.5% glycerol or (C) 99.5% glycerol + 4% formaldehyde. Twenty-three stentless chordally supported bileaflet pericardial mitral valves with treatments A (n = 6), B (n = 6) or C (n = 11) were implanted in juvenile sheep for 125-273 days. After sacrifice, the anterior cusp from the annulus to papillary muscle of each valve was examined by scanning electron microscopy for the presence of endothelial cells, and the intrinsic calcification of each valve was determined by measuring calcium (micrograms/mg dry weight) from another 1 cm2 piece of grossly normal cusp. Sixty pieces of 1 cm2 pericardium with treatment A, B or C (n = 20 in each group) were implanted in 30 rats for 70 days. Calcium analysis and histology study of the implants were performed. In sheep, within a similar range of implantation periods, the endothelial growth rate of the valves was the highest in group B, 100% (6/6); group C was 45.5% (5/11) and A 16.7% (1/6). There were no significant differences in calcium among groups A, B and C. In rat implants, the calcium of group B was much lower than that of A or C (B = 6.92 +/- 4.46 vs A = 144.52 +/- 27.66 or C = 240.54 +/- 13.47, P < 0.05) although its histology showed more severe degeneration and inflammatory changes. Pericardial mitral valves treated with glycerol show satisfactory biocompatibility with regard to host endothelial growth and prevention of calcification; however, these tissues show evidence of rapid degeneration.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cardiothoracic Surgery, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: K
authors.initials: K
authors.lastname: Liao
authors.forename: G
authors.initials: G
authors.lastname: Gong
authors.forename: D
authors.initials: D
authors.lastname: Hoffman
authors.forename: E
authors.initials: E
authors.lastname: Seifter
authors.forename: S M
authors.initials: SM
authors.lastname: Factor
authors.forename: F
authors.initials: F
authors.lastname: Macaluso
authors.forename: R W
authors.initials: RW
authors.lastname: Frater
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D001705:Bioprosthesis / Q000009:adverse effects*; D002114:Calcinosis / Q000209:etiology*; D002417:Cattle; D004195:Disease Models, Animal; D004727:Endothelium / Q000473:pathology; D005976:Glutaral; D005990:Glycerol; D006350:Heart Valve Prosthesis / Q000009:adverse effects*; D008943:Mitral Valve; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep
chemical_list: D001672:Biocompatible Materials; D005990:Glycerol; D005976:Glutaral