Cloning of the human dopamine D5 receptor gene and identification of a highly polymorphic microsatellite for the DRD5 locus that shows tight linkage to the chromosome 4p reference marker RAF1P1.
We identified a cosmid clone with exact sequence homology to part of the human dopamine D5 receptor gene (DRD5) after screening a cosmid library with the human DRD1 gene. The dopamine D5 receptor was mapped to chromosome 4p15.1-p15.3 by in situ hybridization and using a somatic cell hybrid panel. We report here the further localization of the DRD5 gene following identification of a highly polymorphic dinucleotide repeat sequence in the cosmid clone. The microsatellite (D5(CT/GT/GA)n) had 12 alleles with a polymorphic information content value of 0.77. Linkage analysis in 39 CEPH pedigrees demonstrated tight linkage to the chromosome 4p reference marker RAF1P1 (Zmaxf 20.66 at theta f 0.05 and ZmaxM 16.57 at theta m 0.07).
Sherrington
R
R
Academic Department of Psychiatry, University College London Medical School, United Kingdom.
Mankoo
B
B
Attwood
J
J
Kalsi
G
G
Curtis
D
D
Buetow
K
K
Povey
S
S
Gurling
H
H
D001483:Base Sequence; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D004247:DNA; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011954:Receptors, Dopamine / Q000235:genetics*; D017447:Receptors, Dopamine D1; D050641:Receptors, Dopamine D5
D004276:DNA, Satellite; C493605:DRD5 protein, human; D005819:Genetic Markers; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D050641:Receptors, Dopamine D5; D004247:DNA