title: Interleukin 4 inhibits the proliferation and promotes the maturation of human leukemic early B cells.
abstract: The effects of interleukin 4 (IL-4) on human leukemic precursor B-cell lines were investigated. Recombinant IL-4 (rIL-4) was added to three acute lymphoblastic leukemia-derived pre B-cell lines: Reh, Km3 and Nalm-6. Our results show that rIL-4 significantly decreases continuous proliferation of Reh and Km3 cells while Nalm-6 cells have a limited response in this respect. This rIL-4 effect is dose-dependent and can be neutralized by anti-IL-4 monoclonal antibody (mAb). Furthermore, rIL-4 down-regulated IL-3-induced proliferation of Reh cells. Phenotypic analysis of rIL-4-treated cells points to significant induction of surface marker maturation of leukemic cells by this cytokine. Together, these in vitro data suggest that IL-4: 1) inhibits the proliferation and 2) promotes the differentiation of certain human leukemic B-cell precursors.
authors.affiliation: Laboratoire d'Immunologie, CNRS URA625, Unité Claude Bernard C20, Hôpital Pitié-Salpêtrière, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Ouaaz
authors.forename: F
authors.initials: F
authors.lastname: Mentz
authors.forename: M D
authors.initials: MD
authors.lastname: Mossalayi
authors.forename: C
authors.initials: C
authors.lastname: Schmitt
authors.forename: A
authors.initials: A
authors.lastname: Michel
authors.forename: P
authors.initials: P
authors.lastname: Debre
authors.forename: J J
authors.initials: JJ
authors.lastname: Guillosson
authors.forename: H
authors.initials: H
authors.lastname: Merle-Beral
authors.forename: M
authors.initials: M
authors.lastname: Arock
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology*; D002455:Cell Division; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D007376:Interleukin-2; D007377:Interleukin-3; D011994:Recombinant Proteins; D015847:Interleukin-4