Toluene diisocyanate-stimulated release of arachidonic acid metabolites in the organ bath from guinea-pig airways.
This study was designed to evaluate whether metabolites of arachidonic acid play a role in the contractile response to toluene diisocyanate in isolated guinea pig airways. In control experiments we collected the supernatant from an organ bath over a time period of 2 h, after the addition of toluene diisocyanate (100 and 300 microM), and after the addition of toluene diisocyanate (300 microM) in the presence of indomethacin (5 microM). We measured prostaglandin E2, 6-keto-prostaglandin F1 alpha, prostaglandin F2 alpha, thromboxane B2, leukotriene B4, leukotriene C4/D4/E4/F4 by radioimmunoassays. Levels of prostaglandin F2 alpha and 6-keto-prostaglandin F1 alpha increased significantly after addition of toluene diisocyanate in the absence of indomethacin. These results suggest that prostaglandins are involved in toluene diisocyanate-induced contractions in guinea-pig airways.
Mapp
C E
CE
Institute of Occupational Medicine, University of Padua, Italy.
Boniotti
A
A
Masiero
M
M
Plebani
M
M
Burlina
A
A
Papi
A
A
Maestrelli
P
P
Saetta
M
M
Ciaccia
A
A
Fabbri
L
L
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D011453:Prostaglandins / Q000378:metabolism; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology*
D001095:Arachidonic Acids; D011453:Prostaglandins; D014051:Toluene 2,4-Diisocyanate; D007213:Indomethacin