Fluorometric determination of total and bound sulfite in wine by N-(9-acridinyl)maleimide.
N-(9-Acridinyl)maleimide (NAM) reacts with sulfite in wine and gives strong fluorescent derivatives that lead to highly sensitive fluorometry of both total and bound sulfite in wine. Values of free and bound sulfite in wine determined by the NAM method and the modified Rankine method agreed. Sulfite was determined in < 200 microL wine within 2 h.
Akasaka
K
K
Tohoku University, Faculty of Agriculture, Sendai, Japan.
Ohrui
H
H
Meguro
H
H
Suzuki
T
T
Miwa
H
H
D005470:Fluorometry / Q000379:methods*; D008301:Maleimides; D013447:Sulfites / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D008301:Maleimides; D013447:Sulfites; C080490:N-(9-acridinyl)maleimide