Interleukin-6 and transforming growth factor-beta 1 mRNAs are induced in rat facial nucleus following motoneuron axotomy.
Transection of the rat facial nerve leads to a rapid activation of both astrocytes and microglia around axotomized motoneurons. The factors involved in glial activation in vivo are poorly defined but cytokines have been implicated as major regulators of glial activity in vitro. In the present study we have investigated the expression of cytokine mRNAs in the axotomized facial nucleus that might be involved in glial activation. Eight hours after axotomy unilateral transection of the facial nerve had already induced a rapid accumulation of interleukin (IL)-6-mRNA, with a peak at 24 hours. No IL-6 mRNA was detected on the unoperated control side. Transforming growth factor (TGF)-beta 1 mRNA was detected at low levels in the normal facial nucleus, increasing to three times the normal level 2 days after axotomy. After day 7 TGF-beta 1 mRNA levels gradually declined, with a second minor peak 21 days after axotomy. In situ hybridization experiments, 4 and 21 days after axotomy, localized TGF-beta 1 mRNA to activated microglial cells around regenerating motoneurons, as well as probably some astrocytes. Motoneurons did not express TGF-beta 1 mRNA. TGF-beta 3 was found to be normally expressed in the facial nucleus but was not regulated by axotomy. No mRNA for IL-1, tumour necrosis factor-alpha or interferon-gamma was found in the regenerating facial nucleus at any point in time.(ABSTRACT TRUNCATED AT 250 WORDS)
Kiefer
R
R
Department of Neuromorphology, Max-Planck-Institute for Psychiatry, Martinsried, Germany.
Lindholm
D
D
Kreutzberg
G W
GW
D000818:Animals; D001369:Axons / Q000502:physiology; D001933:Brain Stem / Q000378:metabolism*; D003714:Denervation; D005154:Facial Nerve / Q000378:metabolism*; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D007371:Interferon-gamma / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D016212:Transforming Growth Factor beta / Q000235:genetics*
D015850:Interleukin-6; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D007371:Interferon-gamma