Microfibril-associated glycoprotein: characterization of the bovine gene and of the recombinantly expressed protein.
Microfibrils having a diameter of 10-12 nm, found either in association with elastin or independently, are an important component of the extracellular matrix of many tissues. Because isolation of native proteins composing the microfibrils has proven difficult, information on structure/function relationships is limited. In order to extend our understanding of the 31-kDa microfibril-associated glycoprotein (MAGP), the bovine gene has been cloned and characterized and the protein has been expressed in a eukaryotic system. The compact coding portion of the gene is contained in 4.5 kbp of genomic DNA and does not appear to share any domain motifs with other known proteins. The size, amino acid composition, and sequence of the amino terminus of the secreted recombinant protein (rMAGP) all agree with values predicted by the nucleotide sequence of the cDNA used in the expression vector. The rMAGP reacts with a monospecific antibody prepared against a defined amino acid sequence of the natural molecule and reacts specifically with recombinantly produced tropoelastin, suggesting that rMAGP will be a useful reagent with which to study its interaction with other extracellular matrix components.
Bashir
M M
MM
Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104.
Abrams
W R
WR
Rosenbloom
J
J
Kucich
U
U
Bacarra
M
M
Han
M D
MD
Brown-Augsberger
P
P
Mecham
R
R
Rosenbloom
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002417:Cattle; D003285:Contractile Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D004262:DNA Restriction Enzymes; D018076:DNA, Complementary / Q000737:chemistry; D004547:Elastic Tissue; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins; D015870:Gene Expression; D008969:Molecular Sequence Data; D010957:Plasmids; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014333:Tropoelastin / Q000378:metabolism
D003285:Contractile Proteins; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins; D014333:Tropoelastin; C016598:microfibrillar protein; D004247:DNA; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases