Serum malondialdehyde and mitochondrial aspartate aminotransferase activity as markers of chronic alcohol intake and alcoholic liver disease.
Since lipid peroxidation is a well-know mechanism of alcohol-related liver damage, the aim of the present study was to assess the role of serum malondialdehyde (MDA), a secondary product of lipoperoxidation, in the detection of alcoholism and different stages of alcoholic liver disease and to correlate serum levels of malondialdehyde with other markers. Sixty-five patients with a mean alcohol intake of 151 gr/day, were divided into three groups: alcoholics with normal liver function (ANLF, 7 pts), non-cirrhotic alcoholic liver disease (NCALD, 26 pts) and alcoholic cirrhosis (ALC, 32 pts). The control group consisted of 15 healthy subjects. Serum MDA was measured by the thiobarbituric acid reaction test, and mitochondrial aspartate aminotransferase (mAST) with immunochemical assay. MDA had a higher sensitivity (70% vs 37.5%) and specificity (100% vs 93%) than mAST in detecting alcohol abuse, irrespective of the presence of liver disease. Serum MDA levels were significantly higher in all three groups than in controls (2.3 +/- 0.1 nmol/ml), the highest value being found in NCALD (4.6 +/- 0.4). Serum MDA levels were correlated with prothrombin time (p < 0.005) and blood alcohol levels (p < 0.05). mAST serum activity was also significantly higher in all three groups than in controls. A significant correlation was found between serum MDA and mAST only when the whole group was considered.(ABSTRACT TRUNCATED AT 250 WORDS)
Baldi
E
E
Cattedra Malattie Apparato Digerente, Università degli Studi di Padova, Italy.
Burra
P
P
Plebani
M
M
Salvagnini
M
M
D000328:Adult; D000368:Aged; D000437:Alcoholism / Q000175:diagnosis*; D001219:Aspartate Aminotransferases / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D004796:Clinical Enzyme Tests / Q000379:methods; D005260:Female; D006801:Humans; D008108:Liver Diseases, Alcoholic / Q000175:diagnosis*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000737:chemistry / Q000201:enzymology
D015415:Biomarkers; D008315:Malondialdehyde; D001219:Aspartate Aminotransferases