title: Membrane-bound 5'-nucleotidase/nucleoside phosphotransferase from Bacillus cereus.
abstract: 1. A search for nucleoside phosphotransferase activity in Bacillus cereus led to the following results: (i) The phosphotransferase activity was associated with a membrane bound 5'-nucleotidase. (ii) The enzyme phosphorylates both purine and pyrimidine nucleosides as well as 2',3'-dideoxyinosine. (iii) The enzyme was inhibited by adenylic nucleotide di- and triphosphates, and its nucleotidase activity was increased in the presence of inosine as phosphate acceptor. 2. Bacterial and vertebrate 5'-nucleotidases with phosphotransferase activity differ for several characteristics, such as cellular location, substrate specificity, magnesium requirement and regulation.
authors.affiliation: Dipartimento di Fisiologia e Biochimica, Università di Pisa, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Baiocchi
authors.forename: R
authors.initials: R
authors.lastname: Pesi
authors.forename: M
authors.initials: M
authors.lastname: Turriani
authors.forename: M G
authors.initials: MG
authors.lastname: Tozzi
authors.forename: M
authors.initials: M
authors.lastname: Camici
authors.forename: P L
authors.initials: PL
authors.lastname: Ipata
mesh_terms: D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D001409:Bacillus cereus / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; C021892:nucleoside phosphotransferase; D015720:5'-Nucleotidase