Molecular characterization of an aberrant mercury resistance transposable element from an environmental Acinetobacter strain.
We present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pKLH2 from an environmental bacterium, Acinetobacter calcoaceticus, isolated from a mercury mine. The pKLH2 mer operon has essentially the same gene organization as that of Tn21 and Tn501 from clinical bacteria. The pKLH2 mer operon nucleotide sequence shows 85.5% identity with the Tn501 and 80.9% identity with the Tn21 sequences. Vestigial sequences have been found at the ends of the pKLH2 mer operon, indicating that the pKLH2 mer operon was once a part of a Tn21-like transposon, which had committed suicide by an aberrant resolution event.
Kholodii
G Y
GY
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
Gorlenko
Z
Z
Lomovskaya
O L
OL
Mindlin
S Z
SZ
Yurieva
O V
OV
Nikiforov
V G
VG
D000150:Acinetobacter / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005144:F Factor / Q000235:genetics*; D005798:Genes, Bacterial; D008628:Mercury / Q000633:toxicity*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000378:metabolism; D009876:Operon; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D019895:Transposases
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C051124:MerR protein, Bacteria; D009713:Nucleotidyltransferases; D019895:Transposases; D008628:Mercury