title: c-fos antisense generates apomorphine and amphetamine-induced rotation.
abstract: Sodium pentobarbital anaesthetized rats were injected ipsilaterally with an antisense oligonucleotide to c-fos and contralaterally with a sense of oligonucleotide to c-fos in the striatum. Ten hours later they were injected with amphetamine or apomorphine and their behaviour was observed for a further 2 h at which time they were overdosed with anaesthetic and their brains were removed, frozen and processed for Fos and Jun B immunohistochemistry. Rats showed amphetamine- and apomorphine-induced rotation towards the antisense injected striatum. The antisense oligonucleotide also strongly inhibited the amphetamine-induced expression of c-Fos and Jun B in striatal neurones. These results suggest that antisense to c-fos produces a biochemical change in the injected striatum that then, 10 h later, blocks amphetamine- and apomorphine-induced behavioural and biochemical effects.
authors.affiliation: Department of Pharmacology, School of Medicine, University of Auckland, New Zealand.
authors.forename: M
authors.initials: M
authors.lastname: Dragunow
authors.forename: P
authors.initials: P
authors.lastname: Lawlor
authors.forename: B
authors.initials: B
authors.lastname: Chiasson
authors.forename: H
authors.initials: H
authors.lastname: Robertson
mesh_terms: D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D001483:Base Sequence; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos / Q000235:genetics*; D007267:Injections; D008297:Male; D008969:Molecular Sequence Data; D017072:Neostriatum; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012399:Rotation; D013239:Stereotyped Behavior / Q000187:drug effects*
chemical_list: D016376:Oligonucleotides, Antisense; D000661:Amphetamine; D001058:Apomorphine