LH responses to single doses of exogenous GnRH by freshly captured Damaraland mole-rats, Cryptomys damarensis.
Pituitary function in reproductive and nonreproductive colony members of Damaraland mole-rats, Cryptomys damarensis, was investigated by measuring the LH responses to single doses of 2 micrograms exogenous GnRH and physiological saline in 29 females and 37 males (31 of these animals from two entire colonies). In females, basal LH concentrations were significantly greater in reproductive (n = 9) than in nonreproductive animals (n = 11): 7.6 +/- 1.0 versus 4.3 +/- 0.6 miu ml-1, respectively (P < 0.001). Reproductive females had a significantly greater LH response to 2.0 micrograms GnRH (7.6 +/- 1.0 to 37.7 +/- 6.2 miu ml-1; n = 9) than did nonreproductive females (4.3 +/- 0.6 to 11.8 +/- 1.0 miu ml-1; n = 11, P < 0.001). In contrast, there was no significant difference in basal LH concentrations between reproductive (n = 8) and nonreproductive males (n = 20): 5.3 +/- 4.3 versus 3.2 +/- 1.2 miu ml-1, respectively. There was also no difference in LH response to the administration of 2.0 micrograms GnRH between reproductive and nonreproductive males (5.3 +/- 4.3 to 21.8 +/- 8.6 miu ml-1; n = 8; versus 3.2 +/- 1.2 to 21.1 +/- 8.5 miu ml-1; n = 21; P = 0.5). When the results from the two entire colonies were analysed separately, LH responses to GnRH in the 11 nonreproductive females were less than in the two reproductive females. In contrast, the response of two reproductive males in the colonies did not differ from that of 16 nonreproductive males, although these latter comparisons could not be validated statistically.(ABSTRACT TRUNCATED AT 250 WORDS)
Bennett
N C
NC
Department of Zoology, University of Cape Town, Rondebosch, Republic of South Africa.
Jarvis
J U
JU
Faulkes
C G
CG
Millar
R P
RP
D000818:Animals; D001681:Biological Assay; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D008297:Male; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology*; D012377:Rodentia / Q000502:physiology*; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000096:biosynthesis
D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D007986:Luteinizing Hormone