Crystallization and preliminary X-ray investigation of a recombinant outer membrane protein from Neisseria meningitidis.
A protein constituent of the outer membrane from Neisseria meningitidis (hereafter called P64K) has been crystallized using the hanging drop technique. Crystals are tetragonal with unit cell dimensions a = b = 136.84 A and c = 78.44 A, compatible with a single monomer of 64 kDa in the asymmetric unit. When exposed to high intensity synchrotron radiation, these crystals diffract X-rays to at least 2.9 A resolution, indicating that a high resolution structure analysis is feasible.
Li de la Sierra
I
I
CIGB, Cubanacan La Habana, Cuba.
Prangé
T
T
Fourme
R
R
Padrón
G
G
Fuentes
P
P
Musacchio
A
A
Madrazo
J
J
D000942:Antigens, Bacterial / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000648:ultrastructure*; D018360:Crystallography, X-Ray; D009345:Neisseria meningitidis / Q000737:chemistry*; D011994:Recombinant Proteins
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins