Subcloning and restriction enzyme mapping of bovine adenovirus type 2.
Bovine adenoviruses (BAVs), causing both respiratory and/or enteral diseases in cattle, have been isolated in many countries all over the world. In this study we report on the molecular cloning of the internal EcoRI fragments spanning 20.6-90.5%, and the internal SalI fragments spanning 3.1-65.2% of the BAV type 2 (BAV2; strain No. 19) genome into the plasmid vector pUC19. Moreover, the subcloning of the BAV2 genome facilitated the construction of a detailed physical restriction endonuclease map for BamHI, ClaI, EcoRI, HindIII, KpnI, NotI, NspV, PstI, PvuI, SalI, XbaI and XhoI.
Salmon
K
K
Department of Biological Sciences, Brock University, St. Catharines, Ont., Canada.
Esford
L
L
Haj-Ahmad
Y
Y
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D017921:Mastadenovirus / Q000235:genetics*; D015183:Restriction Mapping
D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases