Enhanced proliferation of peripheral blood mononuclear cells and tumor-infiltrating lymphocytes in a plasma-derived cell culture fluid.
The proliferation and activation of murine and human T-lymphocytes in a high-protein lymphocyte culture fluid (LCF) is compared to that of cells cultured in 10% fetal bovine serum (FBS). Tumor-infiltrating lymphocytes (TIL) proliferate exponentially in the LCF for up to 46 days and generate cell numbers that are nearly 100,000-fold greater than cells cultured in FBS. This rapid growth of T cells in LCF could have an important impact in adoptive immunotherapy and gene therapy since cell growth is a limiting factor in these technologies.
Torres
A R
AR
HyClone Laboratories, Logan, UT.
Burger
R
R
Rich
A
A
Spendlove
R
R
Warren
R P
RP
D000818:Animals; D001769:Blood; D002417:Cattle; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005312:Fetal Blood; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008213:Lymphocyte Activation; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology*; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D010982:Platelet-Derived Growth Factor; D011043:Pokeweed Mitogens; D016212:Transforming Growth Factor beta; D003208:Concanavalin A