Discovery of an ovine alpha S2-casein variant.
A novel ovine alpha S2-casein variant has been detected using discontinuous polyacrylamide gel electrophoresis at alkaline pH, two dimensional electrophoresis and immunoblotting. It is characterized by a greater negative net charge and a lower isoelectric point compared with the most common ovine alpha S2-casein variant. The phenotypic frequency in the Manchega breed is 5.5%.
Chianese
L
L
Istituto di Industrie Agrarie, Facoltà di Agraria dell'Università degli Studi di Napoli, Federico II, Portici, Italia.
Garro
G
G
Addeo
F
F
Lopez-Galvez
G
G
Ramos
M
M
D000818:Animals; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification*; D004563:Electrochemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D014644:Genetic Variation; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007526:Isoelectric Point; D008892:Milk / Q000737:chemistry*; D012756:Sheep
D002364:Caseins