[Expression of the vif gene of human immunodeficiency virus type 1 in Escherichia coli and study of the immunoreactivity of the vif protein].
Full-sized gen vif of human immunodeficiency virus has been synthesized and cloned into plasmid pGEX-2T. Vif-gene expression was found in Escherichia coli cells resulting in production of a hybrid GST-protein. The recombinant protein studied by the immunoblotting technique reacted with 8 of 22 probes of human HIV-positive sera. The recombinant protein is specifically cut by thrombin in two proteins corresponding to GST and VIF-proteins in molecular mass.
Karaseva
N G
NG
Gibadulin
R A
RA
Kiselev
S I
SI
Karamov
E V
EV
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D016346:Gene Products, vif / Q000235:genetics* / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D054320:vif Gene Products, Human Immunodeficiency Virus
D017931:DNA Primers; D016346:Gene Products, vif; D011994:Recombinant Proteins; D054320:vif Gene Products, Human Immunodeficiency Virus
Ekspressiia gena vif virusa immunodefitsita cheloveka tipa 1 v kletkakhEscherichia coli i issledovanie immunoreaktivnosti vif-belka.