title: Zinc, a structural component of adenylate kinases from gram-positive bacteria.
abstract: The recent finding that Bacillus stearothermophilus adenylate kinase contains a zinc atom coordinated to four cysteines prompted us to investigate the metal-binding properties of the enzyme from various bacteria. We conclude that zinc was present only in adenylate kinase from gram-positive species and that this property is correlated with the presence of three or four Cys residues in the sequence Cys-X2-Cys-X16-Cys-X2-Cys/Asp, in which X stands for different amino acid residues.
authors.affiliation: Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Gilles
authors.forename: P
authors.initials: P
authors.lastname: Glaser
authors.forename: V
authors.initials: V
authors.lastname: Perrier
authors.forename: A
authors.initials: A
authors.lastname: Meier
authors.forename: R
authors.initials: R
authors.lastname: Longin
authors.forename: M
authors.initials: M
authors.lastname: Sebald
authors.forename: L
authors.initials: L
authors.lastname: Maignan
authors.forename: E
authors.initials: E
authors.lastname: Pistotnik
authors.forename: O
authors.initials: O
authors.lastname: Bârzu
mesh_terms: D000263:Adenylate Kinase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000032:analysis* / Q000378:metabolism
chemical_list: D000263:Adenylate Kinase; D015032:Zinc; D003545:Cysteine