title: Augmentation of intrarenal angiotensin II levels by chronic angiotensin II infusion.
abstract: The objective of this study was to investigate the singular role of elevated angiotensin II (ANG II) levels in the development of two-kidney, one-clip (2K1C) Goldblatt hypertension in the rat and specifically in the altered intrarenal ANG II levels that occur in the nonclipped kidney. As a substitute for the clipped kidney, chronic delivery of ANG II (40 ng/min) via an osmotic minipump implanted subcutaneously was used to mimic plasma ANG II levels observed in 2K1C rats during the developmental phase of hypertension. Arterial pressure increased gradually over a period of 14 days, and a pressure profile similar in magnitude and temporal pattern to that of the 2K1C rats was observed. Systemic ANG II was elevated to similar levels in the 2K1C (60 +/- 13 fmol/ml) and ANG II-infused rats (72 +/- 15 fmol/ml) compared with intact two-kidney control animals (31 +/- 6 fmol/ml; P < 0.05) or uninephrectomized rats (13 +/- 1 fmol/ml; P < 0.05). Although renin content was markedly suppressed (80%), intrarenal ANG II content of the contralateral kidneys of the 2K1C groups (86 +/- 12 fmol/g) and the ANG II-infused group (150 +/- 17 fmol/g) was greater than that of the two-kidney control (53 +/- 7 fmol/g; P < 0.05) and uninephrectomized control animals (42 +/- 5 fmol/g; P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112.
authors.forename: A M
authors.initials: AM
authors.lastname: Von Thun
authors.forename: R C
authors.initials: RC
authors.lastname: Vari
authors.forename: S S
authors.initials: SS
authors.lastname: el-Dahr
authors.forename: L G
authors.initials: LG
authors.lastname: Navar
mesh_terms: D000804:Angiotensin II / Q000378:metabolism* / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004231:Diuresis / Q000187:drug effects; D005919:Glomerular Filtration Rate; D006439:Hemodynamics / Q000187:drug effects; D006978:Hypertension, Renovascular / Q000378:metabolism / Q000503:physiopathology; D007260:Infusion Pumps; D007668:Kidney / Q000378:metabolism*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation; D012084:Renin-Angiotensin System; D013997:Time Factors
chemical_list: D000804:Angiotensin II; D007703:Peptidyl-Dipeptidase A