Expression of cytochrome b558 on B cell- and CD 30 positive-lymphomas.
Expression of cytochrome b558, an essential constituent of the superoxide generating system in phagocytes, was demonstrated in B-lymphocytes. To determine its expression in malignant lymphoma (ML), 103 non-Hodgkin's MLs and 18 Hodgkin's (HD) MLs, together with non-tumorous lymphoid tissues were immunohistochemically analyzed, using two antibodies specific for the cytochrome. In non-tumorous tonsils and lymph nodes, B-lymphocytes, especially in the mantle zones, and histiocytes stained heavily, while T-lymphocytes failed to stain. Among the 55 B-MLs, all follicular lymphomas (9/9) and 18/46 of the diffuse lymphomas were found to express cytochrome b558. Among 48 T-MLs, 46 were unstained by antibodies against the cytochrome. The two cytochrome b558-positive cases were CD 30-positive anaplastic large cell lymphomas. Sixteen of 18 HD patients had CD 30-positive Reed-Sternberg cells which also stained with antibody alpha LC (an anticytochrome b558 antibody). Thus, cytochrome b558, represents a new lymphocyte differentiation antigen.
Shiota
M
M
Department of Pathology, University of Tokyo, Japan.
Mori
S
S
Imajoh-Ohmi
S
S
Nakamura
M
M
Kanegasaki
S
S
Serizawa
H
H
Izumo
T
T
Uehara
T
T
D001402:B-Lymphocytes / Q000737:chemistry; D003573:Cytochrome b Group / Q000032:analysis*; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D017730:Ki-1 Antigen / Q000032:analysis*; D008221:Lymphoid Tissue / Q000737:chemistry; D016393:Lymphoma, B-Cell / Q000737:chemistry* / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000737:chemistry / Q000473:pathology; D019255:NADPH Oxidases; D016539:Reed-Sternberg Cells / Q000737:chemistry; D013601:T-Lymphocytes / Q000737:chemistry
D003573:Cytochrome b Group; D017730:Ki-1 Antigen; C036888:cytochrome b558; D019255:NADPH Oxidases