title: Time-resolved in-vivo fluorescence of photosensitizing porphyrins.
abstract: Various components of photosensitizing porphyrins (e.g. monomers, aggregates, ionic species) have been recently localized in single cells by time-resolved fluorescence microscopy. Novel time-resolving techniques, based on picosecond laser diodes, a frequency-doubled Nd:YAG laser and time-gated microscopic equipment, were used for in-vivo measurements of the chick chorioallantoic membrane (CAM) exhibiting a pronounced vasculature. Changes of the fluorescence decay kinetics after light exposure were correlated with the formation of a photoproduct (Photosan, aminolaevulinic acid) or changes of the intracellular binding sites (tetraphenyl-porphyrins). Fluorescent components with different decay times were shown to be distributed differently within the tissue.
authors.affiliation: Fachhochschule Aalen, Fachbereich Optoelektronik, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schneckenburger
authors.forename: K
authors.initials: K
authors.lastname: König
authors.forename: K
authors.initials: K
authors.lastname: Kunzi-Rapp
authors.forename: C
authors.initials: C
authors.lastname: Westphal-Frösch
authors.forename: A
authors.initials: A
authors.lastname: Rück
mesh_terms: D000482:Allantois / Q000166:cytology; D000622:Aminolevulinic Acid / Q000032:analysis; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000166:cytology; D005321:Extraembryonic Membranes / Q000166:cytology*; D008027:Light; D017319:Photosensitizing Agents / Q000032:analysis* / Q000528:radiation effects; D011166:Porphyrins / Q000032:analysis* / Q000528:radiation effects; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; C016136:tetraphenylporphine sulfonate; D000622:Aminolevulinic Acid