title: Primary central nervous system lymphomas. Immunophenotypic, virologic, and cytogenetic findings of three patients without immune defects.
abstract: BACKGROUND
  Primary central nervous system (PCNS) lymphoma is a relatively rare disease, but an increasing incidence is reported. The Epstein-Barr virus (EBV), which is often found in lymphomas of immunocompromised patients, has been implicated in the development of lymphomas. Many cytogenetic analyses of nodal B cell lymphomas have been performed, but few studies on PCNS lymphomas have been reported.
  
  
  METHODS
  The detection of EBV genome using the polymerase chain reaction (PCR) method and cytogenetic studies were performed, in addition to histopathologic and immunophenotypic approaches in biopsied tissue from three patients with PCNS lymphoma. Immunosuppressive states and exposure to mutagens were not clear in all patients.
  
  
  RESULTS
  Histopathologic examination disclosed a diffuse type of malignant lymphoma in all patients. Immunophenotypic studies revealed B cell phenotype in all patients, two of whom showed positive reaction for CD5. The PCR method revealed no involvement of EBV genome in tumors in any patients. The cytogenetic study showed clonal chromosome abnormalities in all patients, and abnormalities of chromosome 1 (1q21), 6 (-6, 6q15 and 6q21), 7 (-7 and 7p15), and 14 (14q24 and 14q32) were prominent. The t(6;14)(q15;q32) observed in Patient 1 is the first case to be reported in human de novo lymphoma.
  
  
  CONCLUSIONS
  These findings indicate that the causative role of EBV in PCNS lymphoma without immune defects is not clear. The cytogenetic findings were similar to those observed in nodal B-cell lymphoma, suggesting that the origin of PCNS lymphoma cells does not differ from nodal B cell lymphoma cells cytogenetically.
authors.affiliation: Department of Hematology, Nagasaki University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Itoyama
authors.forename: N
authors.initials: N
authors.lastname: Sadamori
authors.forename: K
authors.initials: K
authors.lastname: Tsutsumi
authors.forename: Y
authors.initials: Y
authors.lastname: Tokunaga
authors.forename: H
authors.initials: H
authors.lastname: Soda
authors.forename: M
authors.initials: M
authors.lastname: Tomonaga
authors.forename: S
authors.initials: S
authors.lastname: Yamamori
authors.forename: Y
authors.initials: Y
authors.lastname: Masuda
authors.forename: K
authors.initials: K
authors.lastname: Oshima
authors.forename: M
authors.initials: M
authors.lastname: Kikuchi
mesh_terms: D000368:Aged; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics / Q000382:microbiology*; D002869:Chromosome Aberrations; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000382:microbiology*; D016400:Lymphoma, Large-Cell, Immunoblastic / Q000235:genetics / Q000382:microbiology*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction