The role of O-antigen in susceptibility of Serratia marcescens to non-immune serum.
Selection of phage-resistant strains to three different phages, kappa, FSB3 and FSB55, whose primary receptor was lipopolysaccharide, enabled several spontaneous O-defective mutants to be isolated and characterized. Strains ATCC 274 (0:14), 2170 (0:14) and NIMA (0:6) showed serum resistance, whereas their O-side chain defective mutants exhibited high serum susceptibility. Both alternate and classical activation pathways were involved in serum killing activity in Serratia marcescens.
Palomar
J
J
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Barcelona, Spain.
Montilla
R
R
Fusté
M C
MC
Viñas
M
M
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D003170:Complement Pathway, Alternative / Q000502:physiology*; D003171:Complement Pathway, Classical / Q000502:physiology*; D008070:Lipopolysaccharides / Q000276:immunology*; D009154:Mutation; D011817:Rabbits; D012706:Serratia marcescens / Q000254:growth & development* / Q000276:immunology*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides