Cloning of eggplant hypocotyl cDNAs encoding cytochromes P450 belonging to a novel family (CYP77).
From eggplant hypocotyl tissues we have cloned two closely related cDNAs encoding cytochromes P450 (P450s) by PCR amplification using a primer designed based on the highly conserved sequence among the known eggplant P450s. One cDNA lacks the NH2-terminal short sequence that is present in the other, full-length cDNA. The two predicted protein sequences are 71% identical with each other and show less than 30% identity with any other known P450s. It is concluded that these P450s, which are termed CYP77A1 and -A2, belong to a hitherto unknown P450 family.
Toguri
T
T
Central Laboratory for Key Technology, Kirin Brewery Co. Ltd, Yokohama, Japan.
Tokugawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012639:Seeds; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000201:enzymology* / Q000235:genetics
D018076:DNA, Complementary; D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; C085703:CYP77A1 protein, Solanum melongena; C085704:cytochrome P-450 CYP77A2, Solanum melongena