Detection of point mutations in DNA using capillary electrophoresis in a polymer network.
The use of capillary electrophoresis (CE) in a polymer network for single-strand conformation polymorphism (SSCP) is investigated. SSCP is a method to detect DNA point mutations, essential in the diagnosis of several diseases. The PCR (polymerase chain reaction) amplified p53 gene, a tumour suppressor gene known to be frequently mutated in malignant cells, was subjected to CE analysis. Two single-strand DNA fragments of 372 bp in length differing in only one nucleotide could be separated. We conclude that SSCP using CE in a polymer network is a powerful method for the detection of point mutations in DNA sequences.
Kuypers
A W
AW
Department of Internal Medicine, University Hospital Nijmegen, Netherlands.
Willems
P M
PM
van der Schans
M J
MJ
Linssen
P C
PC
Wessels
H M
HM
de Bruijn
C H
CH
Everaerts
F M
FM
Mensink
E J
EJ
D001483:Base Sequence; D002197:Capillary Action; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods*; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011108:Polymers; D011110:Polymorphism, Genetic
D011108:Polymers; D004247:DNA