A new method for cryopreservation of mouse spermatozoa.
A new method for the cryopreservation of mouse spermatozoa was developed using a modified egg-yolk TES-Tris diluent containing 0.1% sodium lauryl sulfate and 1.25% (v/v) glycerol (mouse sperm cryoprotectant, MSC). Epididymal spermatozoa collected from 10-week-old CBA males were frozen at a rate of 5 degrees C min-1 to 4 degrees C and 50 degrees C min-1 to -70 degrees C using a programmable cell freezer. A percentage of the spermatozoa (25%) regained motility after thawing. In vitro fertilization with frozen-thawed spermatozoa resulted in 50% of oocytes developing to the two-cell stage. These two-cell embryos were placed in the oviducts of pseudopregnant recipients (C57BL/CBA) and 16% developed to be viable fetuses, or in the oviducts of pregnant recipients (MF1) and 17% developed to live offspring.
Penfold
L M
LM
Institute of Zoology, Zoological Society of London, Regent's Park, UK.
Moore
H D
HD
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004624:Embryo Transfer / Q000401:mortality; D005298:Fertility; D005307:Fertilization in Vitro / Q000379:methods; D005328:Fetal Viability; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D013081:Sperm Motility; D013094:Spermatozoa
D003451:Cryoprotective Agents