[Comparison between the effects of (-)- and (+)-gossypol on protein kinase C and protein kinase A].
Protein kinase C(PKC) and protein kinase A(PKA) purified from rat liver were incubated with (-)- and (+)-gossypol. The activity of PKC was significantly inhibited in a concentration-dependent manner by both (-)-gossypol and (+)-gossypol. (-)-gossypol was found to inhibit type I PKA, whereas the inhibitory action of (+)-gossypol for type I PKA was less potent. Both (-)-gossypol and (+)-gossypol showed inhibition for type II PKA only at high concentration. These results suggest that administration of gossypol might impair cellular signal transduction.
Xiao
D M
DM
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
Zhou
W H
WH
Zhang
Y H
YH
Wang
X L
XL
Zhu
B
B
Zhang
J B
JB
Yang
G D
GD
Chen
H C
HC
D000818:Animals; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006072:Gossypol / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry; D008297:Male; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D006072:Gossypol