The adenylate cyclase catalytic domain of Streptomyces coelicolor is carboxy-terminal.
A DNA fragment of Streptomyces coelicolor encoding the carboxy-terminal catalytic domain of adenylate cyclase was cloned, sequenced and expressed in an Escherichia coli cya-defective strain where it produced nanomole levels of cAMP. The amino acid sequence of the enzyme displays similarities with the Brevibacterium liquefaciens pyruvate regulated adenylate cyclase.
Danchin
A
A
Unité de Régulation de l'Expression Génétique, Institut Pasteur, Paris, France.
Pidoux
J
J
Krin
E
E
Thompson
C J
CJ
Ullmann
A
A
D000262:Adenylyl Cyclases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D000242:Cyclic AMP / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D000242:Cyclic AMP; D000262:Adenylyl Cyclases