Gas chromatographic-mass spectrometric determination of plasma and brain cocaine in mice.
A gas chromatographic-mass spectrometric method is described for the determination of cocaine in mouse plasma microsamples and brain. Cocaine and [2H3]cocaine were extracted with pentane-isopropyl alcohol (97:3, v/v), chromatographed on a (5% phenyl) methylpolysiloxane capillary column, and detected by selected-ion monitoring of electron impact generated m/z 182 and 185 fragment ions. The small sample size (50 microliters), simplicity of workup, and high response linearity (mean r = 0.9993) distinguish the method. Cocaine was found in mouse brain at approximately 5 times greater concentration than in plasma after 20 or 40 mg/kg subcutaneous doses.
Patrick
K S
KS
Department of Pharmaceutical Sciences, Medical University of South Carolina, Charleston 29425-2303.
Boggan
W O
WO
Miller
S R
SR
Middaugh
L D
LD
D000818:Animals; D001923:Brain Chemistry; D003042:Cocaine / Q000032:analysis* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results
D003042:Cocaine