Flow cytometric indirect immunofluorescence assay with high sensitivity and specificity for the detection of antibodies to HSV-1 and HSV-2.
Cells infected with HSV-1 or HSV-2 develop viral antigens which can be detected by immunofluorescence. We developed a flow cytometric indirect immunofluorescence assay to detect and quantitate antibodies to HSV-1 and HSV-2 in human sera. Results obtained by flow cytometry for detecting antibodies against HSV-1, when compared with results obtained by ELISA, showed an index of overall agreement of 100%. The correlation between the antibody titers obtained with each method was found to be highly significant. An index of overall agreement equal to 94.1% was observed between results obtained by flow cytometry and by immunofluorescence as concerns the discrimination of HSV-2 positive from negative samples. However, the correlation between antibody titers was found to be not statistically significant. The flow cytometric assay proved to be type-specific.
Caruso
A
A
Institute of Microbiology, University of Brescia, Italy.
Tinti
M
M
Peroni
L
L
Cabibbo
E
E
De Rango
C
C
Manca
N
N
Turano
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D018259:Herpesvirus 1, Human / Q000254:growth & development / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D012680:Sensitivity and Specificity; D014709:Vero Cells
D000914:Antibodies, Viral