A human gene that shows identity with the gene encoding the angiotensin receptor is located on chromosome 11.
We report the cloning of a gene, intronless in its coding region, which we have named APJ. This gene was cloned using the polymerase chain reaction (PCR), with a set of primers designed on the basis of the conservation that members of G protein-coupled receptors (GPCR) have in their transmembrane (TM) regions. The putative receptor protein, APJ, shares closest identity to the angiotensin receptor (AT1) ranging from 40 to 50% in the hydrophobic TM regions of these receptors. The transcripts for this gene were detected in many regions of the brain. PCR analysis of somatic cell lines found APJ-related sequences to be only present on chromosome 11, and high-resolution mapping by fluorescence in situ hybridization (FISH) sublocalized APJ on band q12.
O'Dowd
B F
BF
Addiction Research Foundation, Toronto, Ontario, Canada.
Heiber
M
M
Chan
A
A
Heng
H H
HH
Tsui
L C
LC
Kennedy
J L
JL
Shi
X
X
Petronis
A
A
George
S R
SR
Nguyen
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D011945:Receptors, Angiotensin / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D011945:Receptors, Angiotensin; D004247:DNA