[Effect of human hepatic stimulator substance on intracellular calcium and potassium homeostasis in CCl4-intoxication dissociated hepatocytes].
The human hepatic stimulator substance (hHSS) was extracted from abortive fetal liver according to the method of LaBrecque. The intracellular free calciumion was measured by fluorescent probes Fura-2/AM. Rat hepatocytes were isolated from liver by the method of Seglen and intoxicated by CCl4 vapour, then the changes of [Ca2+]i, the leakage of intracellular K+, ALT and viability of hepatocytes were observed. The results were as follows: The hHSS does exist in the human fetal liver, hHSS could increase the viability of intoxicated hepatocytes, maintain the intracellular calcium homeostasis, and decrease the leakage of intracellular potassium and ALT into the culture medium. These results indicate that hHSS could protect hepatocytes against CCl4 through maintaining calcium homeostasis, preventing potassium leakage and sustaining stability of hepatocyte polasmalemma.
Shao
Q
Q
Department of Physiology, Dalian Medical College.
Mei
M H
MH
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002252:Carbon Tetrachloride Poisoning; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology*; D005110:Extracellular Space / Q000378:metabolism; D005333:Fetus; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D017136:Ion Transport; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D008297:Male; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C073456:hepatic stimulator substance; D000410:Alanine Transaminase; D011188:Potassium; D002118:Calcium