Nucleotide sequence relationships of double-stranded RNAs in flax rust, Melampsora lini.
Flax rust, Melampsora lini strain SP6, contains 11 double-stranded (ds) RNA molecules with a total length of about 25 kbp. The dsRNAs are inherited in three genetic units: the L unit comprising a single 5.2 kbp dsRNA and contained within a 40-nm virus-like particle, and the A and B units each consisting of five dsRNAs (A1-A5, and B1-B5) ranging in size from 1.2 to 2.7 kbp. This paper reports the isolation of a cDNA library representing 10 of the 11 dsRNAs. By nucleic-acid hybridization techniques it has been shown that all ten sequences are unique showing no detectable cross-hybridization with any other dsRNA present in the rust. A near full-length sequence of 1932 bp of the B3 dsRNA is reported and contains several open reading frames, the largest of which comprises most of the molecule.
Dickinson
M J
MJ
CSIRO, Division of Plant Industry, Canberra, ACT, Australia.
Zhang
R
R
Pryor
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012330:RNA, Double-Stranded / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D012330:RNA, Double-Stranded; D012331:RNA, Fungal