SDS-PAGE profiles of somatic proteins from third-stage infective larvae of Ostertagia ostertagi and Cooperia oncophora.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of somatic proteins from third-stage infective larvae of Ostertagia ostertagi and Cooperia oncophora were evaluated using laser densitometry. Several protein bands were present from both parasite preparations. A few bands from each parasite appeared to be unique. Purification of these proteins for use in serologic monitoring of cattle naturally infected with O. ostertagi and C. oncophora should allow circumvention of cross-reactivity between the two genera, which has been reported by others.
Baker
D G
DG
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Gershwin
L J
LJ
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D003429:Cross Reactions; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000032:analysis* / Q000276:immunology; D007411:Intestinal Diseases, Parasitic / Q000469:parasitology / Q000662:veterinary; D007814:Larva / Q000737:chemistry; D007834:Lasers; D008297:Male; D010028:Ostertagia / Q000737:chemistry* / Q000276:immunology; D010029:Ostertagiasis / Q000469:parasitology / Q000662:veterinary; D013045:Species Specificity; D014251:Trichostrongyloidea / Q000737:chemistry* / Q000276:immunology; D014252:Trichostrongyloidiasis / Q000469:parasitology / Q000662:veterinary
D000947:Antigens, Helminth; D015801:Helminth Proteins