The expression of human plasma cholesteryl-ester-transfer protein in HepG2 cells is induced by sodium butyrate. Quantification of low mRNA levels by polymerase chain reaction.
Although human plasma cholesteryl-ester-transfer protein (CETP) is primarily synthesized in the liver, its expression in a number of transformed liver cell lines is very low. However the use of the human hepatoma cell line HepG2 as a model system for the regulation of CETP on mRNA level is facilitated by a quantitative reverse-transcribed polymerase chain reaction. We demonstrate a time-dependent and concentration-dependent 3-4-fold induction of CETP mRNA by sodium butyrate. CETP mass in the medium is also augmented; however, the effect on protein level is less pronounced.
Sperker
B
B
Zentrum für Molekulare Biologie, Universität Heidelberg, Germany.
Mark
M
M
Budzinski
R M
RM
D001483:Base Sequence; D015152:Blotting, Northern; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters / Q000378:metabolism*; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D002087:Butyrates; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters; D006023:Glycoproteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D020148:Butyric Acid