Analysis of virus-specific RNA species and proteins in Freon-113 preparations of the Borna disease virus.
Treatment of homogenates from Borna disease virus (BDV)-infected brain tissue or cell cultures with Freon-113 yielded infectious particles with a buoyant density of 1.16-1.22 g/ml. Positive- and negative-stranded BDV-specific RNA species as well as three virus-specific proteins, known to be present in BDV-infected cell extracts, were demonstrated in these Freon-treated fractions. When the Freon-purified virus preparations were treated with RNase A prior to RNA extraction, only negative-stranded, genomic RNA was detected in Northern blot hybridizations using sense and antisense RNA probes. These data substantiate that BDV is a negative-stranded RNA virus.
Richt
J A
JA
Institut für Virologie, Justus-Liebig-Universität Giessen, Germany.
Clements
J E
JE
Herzog
S
S
Pyper
J
J
Wahn
K
K
Becht
H
H
D000818:Animals; D001891:Borna disease virus / Q000235:genetics*; D002460:Cell Line; D064227:Chlorofluorocarbons, Ethane; D005617:Chlorofluorocarbons, Methane; D004285:Dogs; D008854:Microscopy, Electron; D012367:RNA, Viral / Q000032:analysis*; D051381:Rats; D011917:Rats, Inbred Lew; D014764:Viral Proteins / Q000032:analysis*
D064227:Chlorofluorocarbons, Ethane; D005617:Chlorofluorocarbons, Methane; D012367:RNA, Viral; D014764:Viral Proteins; C007786:1,1,2-trichloro-1,2,2-trifluoroethane