A negatively light-regulated gene from Arabidopsis thaliana encodes a protein showing high similarity to blue copper-binding proteins.
A negatively photo-regulated gene (bcb) has been isolated by means of differential hybridization of a genomic library of Arabidopsis thaliana. In mature plants, a 20-fold increase in the amount of steady-state bcb mRNA can be detected upon 48 h of dark adaption. The expression level of the gene is also dependent upon the developmental stage of the plant. The 21.5-kDa gene product (BCB) shows extensive similarity with blue Cu(2+)-binding proteins such as plastocyanin and stellacyanin. The protein abundance increases only twofold upon dark adaption, which implies the presence of post-transcriptional control. The isolation of a novel negatively photoregulated gene allows us to investigate the complex expression profile of genes responding to the absence of light.
Van Gysel
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Laboratorium voor Genetica, Universiteit Gent, Belgium.
Van Montagu
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Inzé
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D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001400:Azurin / Q000031:analogs & derivatives* / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003300:Copper; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000528:radiation effects; D008027:Light; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; C085507:BCB protein, Arabidopsis; D002352:Carrier Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; C023548:copper-binding protein; C050107:cupredoxin; D001400:Azurin; D003300:Copper