Age and training alter collagen characteristics in fast- and slow-twitch rat limb muscle.
This study evaluated the single and interactive effects of age and training status on selected collagen parameters in two rodent locomotor skeletal muscles contrasting in fiber type composition. Gastrocnemius (GAST) and soleus (SOL) muscles from both trained (10 wk of daily treadmill running) and sedentary young adult (5-mo-old), middle-aged (15-mo-old), and old (23-mo-old) female Fischer 344 rats were evaluated for concentrations of collagen (measured by hydroxyproline concentration ([OH-Pro])) and of the predominant nonreducible lysine aldehyde-derived collagen cross-link hydroxylysylpyridinoline ([HP]). Maximal aerobic capacity was significantly elevated in all three trained groups compared with sedentary age-matched control groups. Slow-twitch SOL had a significantly higher [OH-Pro] than fast-twitch GAST (P < 0.05). Although aging had no effect on [OH-Pro] in GAST, in SOL a significant increase with age was seen (P < 0.02). In sedentary rats both GAST and SOL [HP] increased with age, with this increase being more pronounced for SOL. Additionally, although training had no effect on the aging-associated increase in GAST [HP], it prevented the rise seen in SOL. The observed training-induced reduction in SOL [HP] presumably reflects exercise recruitment and subsequent stimulation of collagen synthesis and degradation rates in this muscle. We conclude that both aging and training affect the extracellular matrix in rodent limb skeletal muscle.
Zimmerman
S D
SD
Human Energy Research Laboratory, University of Wyoming, Laramie 82071.
McCormick
R J
RJ
Vadlamudi
R K
RK
Thomas
D P
DP
D000375:Aging / Q000502:physiology*; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology; D005260:Female; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology; D010805:Physical Conditioning, Animal; D051381:Rats; D011916:Rats, Inbred F344; D013147:Spirometry
D003094:Collagen; D006909:Hydroxyproline