Presence of a regulatory element within the first intron of the human platelet-derived growth factor-A chain gene.
We detected a suppressive element in the first intron of the human platelet-derived growth factor A chain (PDGF-A) gene. Two or more proteins, at least 110-kd and 90-kd proteins, were bound over a wide region of this fragment, and the fragment suppressed the expression of the PDGF-A chain via these proteins in vivo. Since the fragment also had suppressor activity on the promoter of the PDGF-B chain, it may be involved in a suppressive mechanism of gene expression common to PDGF-A and -B chains. Four tandem repeats of CCCCAT(CCCC) and three direct repeats of GGGGAG were observed in this region. The expression of the PDGF-A chain is considered to be regulated by a mechanism involving not only the 5' upstream region but also introns.
Takimoto
Y
Y
Department of Internal Medicine, Hiroshima University.
Kuramoto
A
A
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005809:Genes, Regulator / Q000235:genetics*; D016153:Genes, Suppressor / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D012208:Rhabdomyosarcoma / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D010982:Platelet-Derived Growth Factor; D015500:Chloramphenicol O-Acetyltransferase