Histocompatibility matching for bone marrow transplantation. Donor-recipient pairs in the 4AOHW cell panel.
While the results of unrelated bone marrow transplantation are continually improving, a number of important issues remain: what are the histocompatibility requirements, what genes are involved, what mismatches are acceptable, and what are the best methods for determining donor-recipient match? In this study of material provided through the 4AOHW and the US NMDP, the match between 53 donor-recipient pairs was determined using several different markers within the MHC. The data showed that many apparently well-matched pairs have many mismatches, including mismatches for non-HLA genes (i.e., non-class-I or non-class-II) within the MHC. New methods matching for blocks of DNA around HLA-B and around HLA-DR/DQ are available that are sensitive and identify additional mismatches that are not apparent using conventional typing methods. The 4AOHW cells provide a valuable resource for the comparison and assessment of new matching techniques.
Christiansen
F T
FT
Department of Clinical Immunology, Royal Perth Hospital, Western Australia.
Tay
G
G
Smith
L K
LK
Witt
C S
CS
Petersdorf
E W
EW
Bradley
B
B
Dawkins
R L
RL
D001315:Australia; D016026:Bone Marrow Transplantation / Q000276:immunology*; D016172:DNA Fingerprinting; D006086:Graft vs Host Disease / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology; D015235:HLA-B Antigens / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009693:Nucleic Acid Hybridization; D014019:Tissue Donors
D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens