Metabolism and degradation of betamethasone 17-valerate in homogenized living skin equivalent.
The metabolism of betamethasone 17-valerate was estimated using an artificial living skin equivalent (LSE). Betamethasone 17-valerate, betamethasone 21-valerate and betamethasone were measured by a normal-phase high-performance liquid chromatographic (HPLC) method. Betamethasone 17-valerate was added to the culture medium with or without LSE homogenate. Degradation profiles (%) of betamethasone 17-valerate remaining in the culture medium with skin homogenate did not differ from those without homogenate. However, the conversion of betamethasone 21-valerate to betamethasone was accelerated by skin homogenate, indicating that LSE has a sufficient level of esterase.
Kubota
K
K
Department of Dermatology, University of California San Francisco, School of Medicine 94143-0989.
Ademola
J
J
Maibach
H I
HI
D001623:Betamethasone / Q000031:analogs & derivatives / Q000378:metabolism; D001624:Betamethasone Valerate / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012867:Skin / Q000378:metabolism*
C085708:betamethasone 21-valerate; D001623:Betamethasone; D001624:Betamethasone Valerate