A Trichomonas vaginalis cDNA with partial protein sequence homology with a Plasmodium falciparum excreted protein ABRA.
We have previously isolated two extracellular cysteine proteases (60 kDa, 30 kDa) from the cell filtrate of an isolate of Trichomonas vaginalis. All isolates tested produced a 60 kDa protease as demonstrated by immunoblot with cross-reacting rabbit serum. A T. vaginalis cDNA library was constructed using lambda gt11. A 760 bp T. vaginalis specific cDNA was sequenced and demonstrates partial protein sequence homology with an extracellular cysteine protease of Plasmodium falciparum. These results are consistent with the hypothesis that this protease may be important in the pathogenesis of T. vaginalis infection.
Garber
G E
GE
Department of Medicine, University of Ottawa, Ontario, Canada.
Lemchuk-Favel
L T
LT
Meysick
K C
KC
Dimock
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000201:enzymology / Q000235:genetics*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014246:Trichomonas vaginalis / Q000201:enzymology / Q000235:genetics*
D000953:Antigens, Protozoan; D017931:DNA Primers; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C079596:p101-ABRA protein, Plasmodium falciparum; D003546:Cysteine Endopeptidases