Suppression of development of anti-nuclear antibody and glomerulonephritis in NZB x NZWF1 mice by persistent infection with lactic dehydrogenase virus: possible involvement of superoxide anion as a progressive effector.
The development of anti-nuclear antibody (ANA) and glomerulonephritis (GN) in autoimmune NZB x NZWF1 mice was suppressed by persistent lactic dehydrogenase virus (LDV) infection. This observation was used to study a possible pathogenetic role for the toxic oxygen radical, superoxide anion (O2-), in the progression of ANA and GN. Compared to macrophages from NZB x NZWF1 mice with LDV infection, macrophages from uninfected NZB x NZWF1 mice exhibited an age-related and drastic increase in O2- production in association with the development of the ANA and GN (representing the late stage of disease). NZB x NZWF1 mice with or without LDV infection were then given the O2- scavenger superoxide dismutase (SOD) during the late stage of the disease. Treatment of uninfected NZB x NZWF1 mice with SOD (10,000 units/mouse/day for 3 weeks) protected animals from the development of ANA and GN. SOD treatment also suppressed the development of the lesions in NZB x NZWF1 mice with LDV infection. Our findings suggest that O2- may, at least in part, contribute to the development of ANA and GN in the late stage of disease, and that decreased O2- production in NZB x NZWF1 mice with LDV infection may be responsible for the suppression of the development of ANA and GN in the late stage of the disease.
Hayashi
T
T
Laboratory of Veterinary Pathology, University of Yamaguchi, Japan.
Noguchi
Y
Y
Kameyama
Y
Y
D000375:Aging / Q000276:immunology; D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D018174:Arterivirus Infections / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology / Q000517:prevention & control; D005260:Female; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology* / Q000517:prevention & control; D007074:Immunoglobulin G / Q000032:analysis; D007772:Lactate dehydrogenase-elevating virus; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008814:Mice, Inbred NZB; D013482:Superoxide Dismutase / Q000627:therapeutic use; D013481:Superoxides / Q000276:immunology* / Q000378:metabolism
D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; D013481:Superoxides; D013482:Superoxide Dismutase