title: Development to the blastocyst stage of pig embryos cultured in the amniotic fluid of developing chick embryos.
abstract: Early-stage pig embryos were collected from superovulated donor gilts (60-72 hr post hCG injection) and co-cultured in modified Brinster medium for ovum culture (mBMOC-2) + porcine oviduct epithelial cells (POEC) or in the amniotic fluid of a developing chick embryo (CEAm). The development rate to the compacted morulae or blastocyst stage was significantly higher (P < 0.01) in embryos co-cultured in CEAm (60.0%) than those co-cultured in mBMOC-2 + POEC (18.0%). The results indicate that CEAm could be used for production of compacted morulae or blastocysts from early-stage pig embryos. This culture system could also be valuable in situations where a CO2 controlled incubator is not available.
authors.affiliation: Department of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: M B
authors.initials: MB
authors.lastname: Ocampo
authors.forename: L C
authors.initials: LC
authors.lastname: Ocampo
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: J
authors.initials: J
authors.lastname: Ueda
authors.forename: H
authors.initials: H
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Kanagawa
mesh_terms: D000653:Amniotic Fluid; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002455:Cell Division; D002642:Chick Embryo / Q000166:cytology / Q000502:physiology*; D005260:Female; D007315:Insemination, Artificial; D011247:Pregnancy; D013552:Swine