Platelet-derived growth factor (PDGF)-AB-mediated phosphorylation of PDGF beta receptors.
Platelet-derived growth factor (PDGF) stimulates the proliferation of Balb/c-3T3 fibroblasts through binding and subsequent activation of PDGF receptors. Activation of the PDGF receptors has been proposed to involve receptor dimerization. PDGF-AB has been shown to bind PDGF alpha and beta receptor subunits to form PDGF alpha beta and alpha alpha receptor dimers. In this paper we demonstrate that, following the down-regulation of PDGF alpha receptors, the binding of PDGF-AB to beta receptors occurred at 37 degrees C but not at 4 degrees C. PDGF-AB stimulated the phosphorylation of PDGF beta receptor monomers in cells depleted of PDGF alpha receptors by prior exposure to PDGF-AA.
Coats
S R
SR
Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37232.
Love
H D
HD
Pledger
W J
WJ
D016475:3T3 Cells; D000818:Animals; D015536:Down-Regulation; D046911:Macromolecular Substances; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011485:Protein Binding; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor / Q000145:classification / Q000378:metabolism*; D013696:Temperature
D046911:Macromolecular Substances; D010982:Platelet-Derived Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor