title: Human immunodeficiency virus type 1 infection of human macrophages modulates the cytokine response to Pneumocystis carinii.
abstract: The present studies examined production of the cytokines tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and IL-6 by human monocyte-derived macrophages exposed to Pneumocystis carinii in vitro and the impact of concurrent macrophage infection with human immunodeficiency virus type 1 (HIV-1) on these cytokine responses. Macrophages were infected with the HIV-1 BaL monocytotropic strain for 10 to 14 days and then exposed to P. carinii. At various times following P. carinii treatment, culture supernatants were harvested to assess the cytokine profile. Addition of P. carinii to HIV-uninfected macrophages resulted in augmented production of IL-6, TNF-alpha, and IL-1 beta protein. By contrast, in HIV-infected macrophages exposed to P. carinii, only the release of IL-6 was increased compared with that for HIV-uninfected macrophages, while the levels of TNF-alpha and IL-1 beta decreased. This altered response was confirmed at the molecular level for TNF-alpha mRNA. Preventing physical contact between P. carinii and macrophages by a membrane filter inhibited all cytokine release. Substituting P. carinii with a preparation of P. carinii 95- to 115-kDa major membrane glycoprotein A yielded a response similar to that obtained by addition of intact P. carinii. These results suggest that HIV-1 infection of human macrophages modulates cytokine responses to P. carinii.
authors.affiliation: Division of Pulmonary and Critical Medicine, New England Deaconess Hospital, Boston, Massachusetts.
authors.forename: O
authors.initials: O
authors.lastname: Kandil
authors.forename: J A
authors.initials: JA
authors.lastname: Fishman
authors.forename: H
authors.initials: H
authors.lastname: Koziel
authors.forename: P
authors.initials: P
authors.lastname: Pinkston
authors.forename: R M
authors.initials: RM
authors.lastname: Rose
authors.forename: H G
authors.initials: HG
authors.lastname: Remold
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000209:etiology; D002448:Cell Adhesion / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000276:immunology; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000276:immunology; D011010:Pneumocystis / Q000276:immunology*; D011020:Pneumonia, Pneumocystis / Q000209:etiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D016207:Cytokines; D005656:Fungal Proteins; D007375:Interleukin-1; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha