Differential in vitro methylation and synthesis of the 480-kilodalton corrinoid protein in Methanosarcina barkeri grown on different substrates.
The 480-kDa corrinoid protein was significantly methylated in extracts of acetate- but not methanol-grown cells incubated with 14CH3OH, in part because of its decreased synthesis in cells grown on substrates other than acetate. In addition, a 200-kDa corrinoid protein was methylated in extracts of methanol- but not acetate-grown cells.
Kremer
J
J
Department of Microbiology, Ohio State University, Columbus 43212.
Burchfield
S
S
Frazier
C
C
Krzycki
J
J
D000085:Acetates / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D045728:Corrinoids; D000432:Methanol / Q000378:metabolism; D017021:Methanosarcina barkeri / Q000254:growth & development / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D008970:Molecular Weight; D011166:Porphyrins / Q000378:metabolism*
D000085:Acetates; D001426:Bacterial Proteins; D045728:Corrinoids; D011166:Porphyrins; D008780:Methyltransferases; D000432:Methanol