Facile identification by electrospray mass spectrometry of the insulin fragment A14-21-B17-30 produced by insulin proteinase.
We confirm the cleavage at position B16-17 of porcine insulin which occurs during in vitro digestion by insulin proteinase. The fragment A14-21-B17-30 was purified by reversed-phase high performance liquid chromatography and characterized by electrospray ionization mass spectrometry. Fast-atom bombardment mass spectrometry, on the other hand, failed to detect the presence of this fragment.
Vu
L
L
Département de Biochimie Médicale, Centre Médicale Universitaire, Geneva, Switzerland.
Stöcklin
R
R
Rose
K
K
Offord
R E
RE
D000818:Animals; D007328:Insulin / Q000737:chemistry*; D007339:Insulysin; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000737:chemistry*; D013552:Swine
D007328:Insulin; D010446:Peptide Fragments; C085322:insulin fragment A(14-21)-B(17-30); D007339:Insulysin