title: Sequence and crystallization of Escherichia coli dethiobiotin synthetase, the penultimate enzyme of biotin biosynthesis.
abstract: The enzyme dethiobiotin synthetase (EC 6.3.3.3) has been cloned and over-expressed in Escherichia coli in such a way that milligram quantities are available. The purified enzyme has been subjected to a number of physical and chemical studies, sequenced and most notably it has been crystallized in a form that is suitable for X-ray structure determination. The cell dimensions are a = 72.8 A, b = 49.2 A, c = 61.4 A, beta = 106.2 degrees. The systematic absences are consistent with the monoclinic space group C2 with one polypeptide chain in the asymmetric unit.
authors.affiliation: Department of Biochemistry, University of Edinburgh, Scotland.
authors.forename: D
authors.initials: D
authors.lastname: Alexeev
authors.forename: S M
authors.initials: SM
authors.lastname: Bury
authors.forename: C W
authors.initials: CW
authors.lastname: Boys
authors.forename: M A
authors.initials: MA
authors.lastname: Turner
authors.forename: L
authors.initials: L
authors.lastname: Sawyer
authors.forename: A J
authors.initials: AJ
authors.lastname: Ramsey
authors.forename: H C
authors.initials: HC
authors.lastname: Baxter
authors.forename: R L
authors.initials: RL
authors.lastname: Baxter
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001710:Biotin / Q000096:biosynthesis; D019731:Carbon-Nitrogen Ligases; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008025:Ligases / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data
chemical_list: D001710:Biotin; D008025:Ligases; D019731:Carbon-Nitrogen Ligases; C020024:dethiobiotin synthetase