Biochemical characterisation of Trichinella spiralis and T. pseudospiralis stichocyte antigens.
One and two dimensional Western blots of Trichinella spiralis and Trichinella pseudospiralis extracts were probed with a monoclonal antibody (7C2C5) which recognizes molecules found in the stichocytes. Three major antigens were revealed in excreted:secreted (E/S) preparations at approx Mr 45, 53 & 60 X 10(3). The Mr 45 X 10(3) molecule was the most abundant secreted antigen, on two dimensional analysis it resolved into many isoforms with a pI range 4.5-6.4 in T. spiralis and into four isoforms pI range 5.1-5.7 in T. pseudospiralis.
Zhang
Y W
YW
Department of Pure and Applied Biology, University of Leeds, United Kingdom.
Lee
D L
DL
Smith
J E
JE
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D007519:Isoantigens / Q000032:analysis; D007814:Larva / Q000276:immunology; D051379:Mice; D014234:Trichinella / Q000276:immunology*; D017160:Trichinella spiralis / Q000276:immunology*
D000947:Antigens, Helminth; D007519:Isoantigens