Thin-layer chromatographic determination of brain catecholamines and 5-hydroxytryptamine.
A simple and sensitive (up to nanogram level) method to determine norepinephrine, serotonin and dopamine in rat brain is described. The amines are acetylated and the derivatives are resolved by thin layer chromatography (TLC) on silica high performance thin layer chromatography (HPLC) plates. Quantification is achieved by fluorescence densitometry at 415 nm excitation wavelength.
Alemany
G
G
Department de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Nicolau
M C
MC
Gamundí
A
A
Rial
R
R
D000818:Animals; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004298:Dopamine / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D012701:Serotonin / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D002395:Catecholamines; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine