title: Direct determination of opium alkaloid-bovine serum albumin conjugate by matrix-assisted laser desorption/ionization mass spectrometry.
abstract: Opium alkaloids (thebaine, codeine and morphine) have been conjugated with bovine serum albumin (BSA) to give individual antigen conjugates which are analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It became clear that 9 molecules of thebaine were contained in a thebaine-BSA conjugate. Codeine and morphine contents in individual conjugates were determined to be 12 and 6 molecules, respectively.
authors.affiliation: Faculty of Pharmaceutical Science, Kyushu University, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shoyama
authors.forename: T
authors.initials: T
authors.lastname: Fukada
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Kusai
authors.forename: K
authors.initials: K
authors.lastname: Nojima
mesh_terms: D003061:Codeine / Q000032:analysis*; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods; D009020:Morphine / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013797:Thebaine / Q000032:analysis*
chemical_list: D012710:Serum Albumin, Bovine; D013797:Thebaine; D009020:Morphine; D003061:Codeine