title: Enterocytic differentiation of the human Caco-2 cell line is correlated with down-regulation of fibronectin and laminin.
abstract: Human intestinal Caco-2 cells were used to examine the expression of fibronectin (FN) and laminin (LN) during enterocytic differentiation. Combination of immunoprecipitation, Western and Northern blotting revealed that Caco-2 cells expressed a classical FN and a variant form of LN: besides B1 and B2 chains, LN contained a 350-kDa heavy chain instead of the 400-kDa A chain. Throughout Caco-2 cell differentiation, FN and LN synthesis decreased at both mRNA and protein levels. These data indicate that enterocytic differentiation involves both transcriptional and/or post-transcriptional down-regulation of FN and LN gene expression.
authors.affiliation: Laboratoire de Biochimie-Biologie cellulaire, INSERM-U 181, Faculté Médecine Saint Antoine, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Levy
authors.forename: O
authors.initials: O
authors.lastname: Loreal
authors.forename: A
authors.initials: A
authors.lastname: Munier
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: J
authors.initials: J
authors.lastname: Picard
authors.forename: G
authors.initials: G
authors.lastname: Cherqui
authors.forename: B
authors.initials: B
authors.lastname: Clement
authors.forename: J
authors.initials: J
authors.lastname: Capeau
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D003470:Culture Media / Q000378:metabolism; D015536:Down-Regulation; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D007797:Laminin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D010452:Peptide Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D003470:Culture Media; D005353:Fibronectins; D007797:Laminin; D012333:RNA, Messenger