Direct determination of opium alkaloid-bovine serum albumin conjugate by matrix-assisted laser desorption/ionization mass spectrometry.
Opium alkaloids (thebaine, codeine and morphine) have been conjugated with bovine serum albumin (BSA) to give individual antigen conjugates which are analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It became clear that 9 molecules of thebaine were contained in a thebaine-BSA conjugate. Codeine and morphine contents in individual conjugates were determined to be 12 and 6 molecules, respectively.
Shoyama
Y
Y
Faculty of Pharmaceutical Science, Kyushu University, Fukuoka, Japan.
Fukada
T
T
Tanaka
T
T
Kusai
A
A
Nojima
K
K
D003061:Codeine / Q000032:analysis*; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods; D009020:Morphine / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013797:Thebaine / Q000032:analysis*
D012710:Serum Albumin, Bovine; D013797:Thebaine; D009020:Morphine; D003061:Codeine