How does the T-DNA of Agrobacterium tumefaciens find its way into the plant cell nucleus?
Agrobacterium tumefaciens causes the crown gall disease in plants by transferring a piece of DNA, the T-DNA, into the genome of the plant cell. The virulence protein VirD2, tightly linked to the T-DNA, is thought to direct it to the plant cell nucleus and to assist it in integration. The VirD2 protein contains two nuclear localization signals (NLS) which are functional both in yeast and in plant cells. One signal is located in the N-terminal part of the protein and resembles a single-cluster type NLS. The second signal is near the C-terminus and is a bipartite type NLS. The involvement of the C-terminal NLS in the entry of the T-DNA into the plant cell nucleus was directly tested in vivo.
Koukolíková-Nicola
Z
Z
Friedrich Miescher-Institut, Basel, Switzerland.
Hohn
B
B
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000472:pathogenicity; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C036483:T-DNA; D037521:Virulence Factors