Carbohydrate enriched diets and bacterial glycosidases in rat faeces.
The expression of some faecal hydrolytic enzymes in rats fed for 4 months on sucrose or starch enriched diets was compared with a standard diet. The assay reliability was confirmed for animal and experimental variability. The beta-D-glucuronidase and beta-D-glucosidase activities were always higher in rats fed on starch than on other diets. N-acetyl-beta-D-glucosaminidase and N-acetyl-beta-D-galactosaminidase showed decreased activity when passing from a standard to a sucrose, and from a sucrose to a starch diet. There was little modification in the levels of faecal alpha-D-glucosidase, sulphatase and protease with the various experimental diets.
Cenci
G
G
Department of Hygiene, University of Perugia, Italy.
Caldini
G
G
Mastrandrea
V
V
Votoni
M R
MR
D000818:Animals; D001419:Bacteria / Q000201:enzymology*; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000378:metabolism; D005243:Feces / Q000201:enzymology*; D005526:Food, Formulated; D006026:Glycoside Hydrolases / Q000032:analysis*; D007422:Intestines / Q000382:microbiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004040:Dietary Carbohydrates; D006026:Glycoside Hydrolases