Delay in expression of a mammary tumor provirus is responsible for defective clonal deletion during postnatal period.
A gene-encoding ligand for deletion of T cells bearing TcRV beta 6 and V beta 8.1 cosegregates a new mammary tumor provirus locus, Mtv-50 in NC mice. The sequence of the open reading frame (ORF) in the 3' long terminal repeat (LTR) of Mtv-50 was strikingly similar to those of Mtv-7, Mtv-43 and exogenous mouse mammary tumor virus (SW) with properties of minor lymphocyte stimulating antigen 1a. Consistent with previous reports, clonal deletion of mature thymocytes bearing TcRV beta 6 was defective during the early postnatal period of mice carrying Mtv-50. Appreciable levels of mRNA corresponding to common Mtv ORF and Mtv-6 ORF were expressed in the neonatal thymus, while little, if any, mRNA corresponding to Mtv-50 ORF was detected in the thymus at the early postnatal stage. Delay in expression of Mtv-50 ORF during the postnatal period may be responsible for the failure of clonal deletion of V beta 6-T cells in the early postnatal life of mice carrying Mtv-50.
Niimi
N
N
Department of Oral Surgery, Nagoya University School of Medicine, Japan.
Wajjwalku
W
W
Ando
Y
Y
Tomida
S
S
Takeuchi
M
M
Ueda
M
M
Kaneda
T
T
Yoshikai
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology / Q000382:microbiology; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D015967:Gene Expression Regulation, Viral; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D016747:Minor Lymphocyte Stimulatory Antigens / Q000235:genetics*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D018089:Superantigens / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology
D017931:DNA Primers; D016747:Minor Lymphocyte Stimulatory Antigens; D012367:RNA, Viral; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens