Optimization of peptide synthesis on polyethylene rods.
Multipin solid-phase peptide synthesis is widely used for epitope mapping of monoclonal and polyclonal antibodies. However, neither the chemical yield nor the homogeneity of products currently match those of solid-phase synthesis of peptides on resins. In order to improve synthesis parameters, we have repeated the standard procedure and introduced modifications during synthesis of model heptapeptides and peptides from the sequence of rhodopsin and other proteins. Good incorporation of amino acids using the multipin peptide synthesis system can now be obtained in less synthesis time and with less costly reagents.
Arendt
A
A
Department of Ophthalmology, University of Florida, Gainesville 32610.
McDowell
J H
JH
Hargrave
P A
PA
D000081:Acetamides; D000595:Amino Acid Sequence; D000596:Amino Acids; D004126:Dimethylformamide; D005449:Fluorenes; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis; D010455:Peptides / Q000138:chemical synthesis*; D011095:Polyethylenes; D012243:Rhodopsin / Q000737:chemistry; D012997:Solvents
D000081:Acetamides; D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D009842:Oligopeptides; D010455:Peptides; D011095:Polyethylenes; D012997:Solvents; D004126:Dimethylformamide; D012243:Rhodopsin; C013959:dimethylacetamide