title: The 100-kDa protein, whose phosphorylation precedes the fusion of chick embryonic myoblasts, is the eukaryotic elongation factor-2.
abstract: We have previously shown that Ca2+/calmodulin-dependent phosphorylation of the 100-kDa protein dramatically increases during the early period of myoblast fusion and inhibition of the protein phosphorylation prevents the fusion. Here, we show that the protein phosphorylation occurs exclusively at Thr residue(s) and the purified 100-kDa protein can be ADP-ribosylated upon treatment with diphtheria toxin. Furthermore, the 13 N-terminal amino acid sequence of the 100-kDa protein, N-Val-Asn-Phe-Val-Asp-Gln-Ile-Arg-Ala-Ile-Met-Asp-Lys, exactly matches with that of elongation factor-2 from rat and hamster. These results indicate that the 100-kDa protein in chick embryonic myoblasts is identical to the eukaryotic elongation factor-2.
authors.affiliation: Department of Molecular Biology and SRC for Cell Differentiation, College of Natural Sciences, Seoul National University, Korea.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Jeon
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: M S
authors.initials: MS
authors.lastname: Kang
authors.forename: C H
authors.initials: CH
authors.lastname: Chung
authors.forename: D B
authors.initials: DB
authors.lastname: Ha
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D008561:Membrane Fusion; D008970:Molecular Weight; D009132:Muscles / Q000196:embryology / Q000378:metabolism / Q000502:physiology*; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000302:isolation & purification / Q000378:metabolism*; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D000596:Amino Acids; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D010750:Phosphoproteins; D000246:Adenosine Diphosphate Ribose; D000255:Adenosine Triphosphate