title: Histomorphometric analyses of cancellous bone from COSMOS 2044 rats.
abstract: The influence of 14 days of spaceflight on cancellous bone of male Wistar rats was assessed by histomorphometric analysis. In proximal tibia, no difference was found between flight, synchronous, vivarium, and basal groups for epiphyseal bone volume or trabecular arrangement. In metaphysis, primary spongiosa width was reduced in flight rats, suggesting an alteration in bone longitudinal growth. In flight rats, secondary spongiosa evidenced a trend toward decreased bone mass, trabecular number and thickness, and osteoid surfaces, whereas there was a tendency toward increased osteoclast number compared with vivarium control but not with synchronous rats. In femoral fossa trochanteri, an area facing deep muscular insertions, no changes in bone volume or structure were noted among the different groups. However, a reduction of osteoid surfaces was seen in flight and synchronous groups compared with the other groups. Resorption activity was increased in flight rats compared with control rats. In thoracic vertebral body, osteoblastic surfaces decreased similarly in flight and synchronous rats. In lumbar vertebral body, decreased osteoblastic surfaces and increased osteoclastic parameters were observed in flight and synchronous rats. The more striking effects of spaceflight were the decrease in tibial primary spongiosa width and the increase in resorption activity of the femoral fossa trochanteri. In all other sites, cellular alterations appeared similar in flight and synchronous rats, suggesting a role for physiological stress. The time course of events would depend on initial growth and turnover rates of bone, its weight-bearing function on earth, and the presence of muscular insertions.
authors.affiliation: Laboratoire de Biologie du Tissu Osseux, University of Medicine, Saint-Etienne, France.
authors.forename: L
authors.initials: L
authors.lastname: Vico
authors.forename: S
authors.initials: S
authors.lastname: Bourrin
authors.forename: C
authors.initials: C
authors.lastname: Genty
authors.forename: S
authors.initials: S
authors.lastname: Palle
authors.forename: C
authors.initials: C
authors.lastname: Alexandre
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000502:physiology; D005269:Femur / Q000033:anatomy & histology / Q000502:physiology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013026:Space Flight; D013904:Thoracic Vertebrae / Q000033:anatomy & histology / Q000502:physiology; D013977:Tibia / Q000033:anatomy & histology / Q000502:physiology; D014893:Weightlessness / Q000009:adverse effects