Precipitation with KCl improves the chemical cross-linking of GST-fusion proteins to agarose after solubilization with SDS.
Huang
Y
Y
College of Pharmacy, University of Arizona, Tucson.
Vanscheeuwijck
P
P
Regan
J W
JW
D011232:Chemical Precipitation; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005941:Glucokinase / Q000737:chemistry / Q000235:genetics; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011189:Potassium Chloride; D011942:Receptors, Adrenergic, alpha / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D012685:Sepharose / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
D003432:Cross-Linking Reagents; D011942:Receptors, Adrenergic, alpha; D011993:Recombinant Fusion Proteins; D012967:Sodium Dodecyl Sulfate; D011189:Potassium Chloride; D012685:Sepharose; D005982:Glutathione Transferase; D005941:Glucokinase