title: High-performance liquid chromatographic analysis of monoamines in the cestode Diphyllobothrium dendriticum.
abstract: The presence of biogene monoamines in adult and larval Diphyllobothrium dendriticum (Cestoda) was investigated by high-performance liquid chromatography with electrochemical detection (HPLC-ED). The biogene amines serotonin (5-HT), dopamine (DA), noradrenaline (NA), and adrenaline (A) as well as many of their precursors and metabolites, comprising a total of 15 different substances, were analyzed. 5-HT, DOPA, DA, NA, and A were detected in the worm, with 5-HT, DOPA, and DA being the dominating amines. The DA metabolites DOPAC and 3-MT or the 5-HT precursor 5-hydroxytryptophan could not be detected, but two unidentified substances, believed to be catecholic, were present in the worm. A high concentration of DOPA was measured in the proglottids and especially in the eggs. This is the first report of A in a flatworm.
authors.affiliation: Department of Biology, Abo Akademi University, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Eriksson
authors.forename: M
authors.initials: M
authors.lastname: Gustafsson
authors.forename: G
authors.initials: G
authors.lastname: Akerlind
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004170:Diphyllobothrium / Q000737:chemistry*; D004298:Dopamine / Q000032:analysis; D007814:Larva / Q000737:chemistry; D012701:Serotonin / Q000032:analysis
chemical_list: D015306:Biogenic Monoamines; D012701:Serotonin; D004295:Dihydroxyphenylalanine; D004298:Dopamine