Transposon mutagenesis of Clostridium acetobutylicum P262: isolation and characterization of solvent deficient and metronidazole resistant mutants.
An efficient transposon mutagenesis system using conjugative transposons Tn916 and Tn925::Tn917 was established for Clostridium acetobutylicum P262, an industrial strain which has proved difficult to manipulate genetically. Transposon insertions occurred at several different locations to produce a variety of mutants. An oligosporogenous mutant deficient in acetone and butanol production, and two sporulation-deficient and metronidazole resistant mutants were characterized with respect to differentiation and solvent production. Tn925::Tn917 inserted near a string of adenosine residues and transposon insertion was often multiple.
Babb
B L
BL
Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
Collett
H J
HJ
Reid
S J
SJ
Woods
D R
DR
D001483:Base Sequence; D003013:Clostridium / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D004251:DNA Transposable Elements / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D008795:Metronidazole / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012997:Solvents / Q000378:metabolism*; D013171:Spores, Bacterial
D004251:DNA Transposable Elements; D012997:Solvents; D008795:Metronidazole