Development to the blastocyst stage of pig embryos cultured in the amniotic fluid of developing chick embryos.
Early-stage pig embryos were collected from superovulated donor gilts (60-72 hr post hCG injection) and co-cultured in modified Brinster medium for ovum culture (mBMOC-2) + porcine oviduct epithelial cells (POEC) or in the amniotic fluid of a developing chick embryo (CEAm). The development rate to the compacted morulae or blastocyst stage was significantly higher (P < 0.01) in embryos co-cultured in CEAm (60.0%) than those co-cultured in mBMOC-2 + POEC (18.0%). The results indicate that CEAm could be used for production of compacted morulae or blastocysts from early-stage pig embryos. This culture system could also be valuable in situations where a CO2 controlled incubator is not available.
Ocampo
M B
MB
Department of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Ocampo
L C
LC
Suzuki
K
K
Mori
T
T
Ueda
J
J
Shimizu
H
H
Kanagawa
H
H
D000653:Amniotic Fluid; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002455:Cell Division; D002642:Chick Embryo / Q000166:cytology / Q000502:physiology*; D005260:Female; D007315:Insemination, Artificial; D011247:Pregnancy; D013552:Swine