cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+.
Human N-acetylglucosaminyltransferase V (GnT-V, EC 2.4.1.155) cDNA was isolated from a human fetal liver cDNA library. Oligonucleotide primers for polymerase chain reaction were designed according to the amino acid sequence of human GnT-V. Screening for the cDNA was carried out by plaque hybridization using PCR products of about 500 bp. Human GnT-V has 741 amino acids and six putative N-glycosylation sites. The homology to rat GnT-V is 88% at the nucleotide level and is 97% at the amino acid level, and there is one amino acid insertion. Using the cDNA clones as probe, five overlapping genomic clones have been isolated from a human phagemid DNA library. The GnT-V gene has been mapped to chromosome 2q21 using fluorescence in situ hybridization.
Saito
H
H
Department of Biochemistry, Osaka University Medical School, Japan.
Nishikawa
A
A
Gu
J
J
Ihara
Y
Y
Soejima
H
H
Wada
Y
Y
Sekiya
C
C
Niikawa
N
N
Taniguchi
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018288:Carcinoma, Small Cell; D002460:Cell Line; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism*; D005333:Fetus; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000201:enzymology*; D008175:Lung Neoplasms; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase