title: [Determination of the optimal condition of hemoprotein immobilization on the polystyrene surface].
abstract: The effect of pH, buffer composition, salts concentration and protein content on the immobilization efficiency of peroxidase, hemoglobin and their conjugates with bovine serum albumin (BSA) have been studied. All the substances were immobilized on the surface of polystyrene matrix. When using the 0.01 M carbonate-bicarbonate buffer (pH 9.6) immobilization of soluble substances is optimal. It was shown that the sorption efficiency of hemoproteins and their conjugates with BSA is considerably enhanced by drying solution containing ammonium bicarbonate. The increase of ammonium bicarbonate concentration from 0.13 M to 0.5 M leads to the process intensification.
authors.forename: N F
authors.initials: NF
authors.lastname: Starodub
authors.forename: L M
authors.initials: LM
authors.lastname: Samokhina
authors.forename: A E
authors.initials: AE
authors.lastname: Rachkov
mesh_terms: D000327:Adsorption; D001639:Bicarbonates; D004800:Enzymes, Immobilized; D006454:Hemoglobins / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011137:Polystyrenes / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D012995:Solubility
chemical_list: D001639:Bicarbonates; D004800:Enzymes, Immobilized; D006454:Hemoglobins; D011137:Polystyrenes; D012709:Serum Albumin; C027043:ammonium bicarbonate; D006735:Horseradish Peroxidase
vernacular_title: Opredelenie optimal'nykh usloviĭ immobilizatsii gemoproteidov na poverkhnosti polistirolovykh plashek.