title: Attenuation by heat stress of a submaximal calcium paradox in the rabbit heart.
abstract: Heat stress limits the injury associated with myocardial ischaemia and reperfusion, an effect previously attributed to enhanced endogenous anti-oxidant activity. We examined the influence of heat stress on the calcium paradox, an injury in which oxidant stress is not thought to play a major role. Twenty-four hours following sham or true heat stress, rabbits were re-anaesthetized and hearts either removed for stress protein analysis (n = 8), or Langendorff-perfused (n = 20) and subjected to a calcium paradox. Ten minutes following calcium repletion (Ca2+ = 1.3 mM), left ventricular developed pressure was better preserved in heat stress vs control hearts (38.3 +/- 5.0 vs 18.8 +/- 4.1 mmHg, respectively, P = 0.003) whilst contracture, measured by left ventricular end-diastolic pressure, was diminished (21.6 +/- 4.7 vs 39.9 +/- 5.2 mmHg, respectively, P = 0.02). Creatine phosphokinase release at 1 min was less in heat stress vs control hearts (10.6 +/- 8.6 vs 86.4 +/- 33.7 U/min/g, respectively, P = 0.01). The myocardial content of the 72 kDa stress protein was elevated eight-fold in heat stress vs control hearts (2.8 +/- 0.2 vs 0.4 +/- 0.1 U, respectively, P = 0.01). This study suggests that some portion of the stress protein response represents a form of cardiac adaptation capable of limiting myocyte injury independent of antioxidant mechanisms.
authors.affiliation: Hatter Institute For Cardiovascular Studies, University College London Medical School, UK.
authors.forename: M S
authors.initials: MS
authors.lastname: Marber
authors.forename: J M
authors.initials: JM
authors.lastname: Walker
authors.forename: D S
authors.initials: DS
authors.lastname: Latchman
authors.forename: D M
authors.initials: DM
authors.lastname: Yellon
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism*; D011817:Rabbits
chemical_list: D006360:Heat-Shock Proteins; D002118:Calcium