title: Dietary cholesterol supplementation improves growth and behavioral response of pigs selected for genetically high and low serum cholesterol.
abstract: We hypothesized that, in pigs selected for low (L) or high (H) serum cholesterol for four generations, neonatal endogenous cholesterol synthesis would be sufficient to meet requirements for brain and body growth. In Experiment 1, eight 16-wk-old L pigs received a diet with or without 200 mg cholesterol/100 g diet for 35 d. Supplemented pigs grew approximately 25% faster and had a significantly greater concentration of free cholesterol in the cerebrum. In Experiment 2, 16 H and 16 L newborn pigs were fed a milk replacer with or without 200 mg cholesterol/100 g diet for 28 d. Pigs fed cholesterol had greater average daily gain (P < or = 0.09), significantly reduced liver 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity, and significantly increased cerebral cholesterol content than pigs not fed cholesterol. One of three indices of exploratory behavior was significantly greater in the L pigs that received cholesterol compared with L pigs that did not receive cholesterol. These data suggest that these neonatal pigs are unable to produce sufficient cholesterol to meet requirements for normal growth and brain development and are dependent on dietary cholesterol in milk.
authors.affiliation: USDA-ARS Children's Nutrition Research Center, Baylor College of Medicine, Houston, TX 77030.
authors.forename: P A
authors.initials: PA
authors.lastname: Schoknecht
authors.forename: S
authors.initials: S
authors.lastname: Ebner
authors.forename: W G
authors.initials: WG
authors.lastname: Pond
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: V
authors.initials: V
authors.lastname: McWhinney
authors.forename: W W
authors.initials: WW
authors.lastname: Wong
authors.forename: P D
authors.initials: PD
authors.lastname: Klein
authors.forename: M
authors.initials: M
authors.lastname: Dudley
authors.forename: J
authors.initials: J
authors.lastname: Goddard-Finegold
authors.forename: H J
authors.initials: HJ
authors.lastname: Mersmann
mesh_terms: D000821:Animal Feed; D000818:Animals; D000833:Animals, Suckling; D001522:Behavior, Animal; D002784:Cholesterol / Q000096:biosynthesis / Q000097:blood*; D002791:Cholesterol, Dietary / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005527:Food, Fortified; D006903:Hydroxymethylglutaryl CoA Reductases / Q000032:analysis; D006937:Hypercholesterolemia / Q000235:genetics*; D007583:Jejunum / Q000201:enzymology; D008099:Liver / Q000201:enzymology / Q000254:growth & development; D008297:Male; D009929:Organ Size; D012641:Selection, Genetic; D002785:Sterol O-Acyltransferase / Q000032:analysis; D013552:Swine; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain
chemical_list: D002791:Cholesterol, Dietary; D014280:Triglycerides; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases; D002785:Sterol O-Acyltransferase