title: Chromosomal localization of the mouse titin gene and its relation to "muscular dystrophy with myositis" and nebulin genes on chromosome 2.
abstract: In the mouse, the genes for the structural components of the myofibril titin and nebulin, Ttn and Neb, map to proximal Chr 2, as does the gene for a muscle disease, "muscular dystrophy with myositis," mdm. To facilitate the evaluation of Ttn and Neb as possible candidates for mdm, we have determined their relative map positions, using a Mus spretus/Mus musculus interspecific backcross. The gene order (distances in cM) cenVim-16.9 +/- 4.7-Neb-7.6 +/- 3.0-Ttn, Acra-18.0 +/- 4.9-Pax-6-17.7 +/- 4.9-a ... has been determined. Considering the standard deviations, Neb, Ttn, and Acra could colocalize with mdm. Using Ttn and Neb probes, DNAs from mdm/mdm and mdm/+ mice were tested for restriction fragment variants in comparison to the M. musculus wildtype. No variants have been found with 11 restriction nucleases. Our data corroborate a conserved synteny comprising genes NEB, TTN, CHRNA1 on human Chr 2q.
authors.affiliation: Developmental Biology Unit, University of Bielefeld, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Müller-Seitz
authors.forename: K
authors.initials: K
authors.lastname: Kaupmann
authors.forename: S
authors.initials: S
authors.lastname: Labeit
authors.forename: H
authors.initials: H
authors.lastname: Jockusch
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D064211:Connectin; D017124:Conserved Sequence; D017931:DNA Primers; D006239:Haplotypes; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics; D009124:Muscle Proteins / Q000235:genetics*; D009137:Muscular Dystrophy, Animal / Q000150:complications / Q000235:genetics*; D009220:Myositis / Q000150:complications / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011494:Protein Kinases; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D064211:Connectin; D017931:DNA Primers; D008565:Membrane Proteins; D009124:Muscle Proteins; C577285:TTN protein, human; C035620:nebulin; D011494:Protein Kinases