title: Immunohistochemical localization of estradiol and progesterone receptors in human uterus throughout pregnancy: expression in endometrial blood vessels.
abstract: Although progesterone and estrogens are essential to maintain human pregnancy after implantation, the localization of their specific receptors in different uterine cell types during pregnancy has not been investigated. We studied uteri (n = 40) obtained during the first 3 months of pregnancy (n = 21) and in late pregnancy (n = 9) as well as from women 5-14 weeks pregnant (n = 10) who had received the antiprogestagen RU 38486 (Roussel-UCLAF) to induce cervical dilation. Frozen tissues were processed for indirect immunocytochemical staining with specific monoclonal antibodies against estrogen receptors (ER; Abbott Laboratories) and progesterone receptors (PR; Li 417). Specific staining for steroid receptors was only detected in the nucleus. In the endometrium, PR staining remained fairly constant throughout pregnancy, whereas ER staining was initially weak and then undetectable. PR was widely expressed in stromal cells and in spiral arterial wall cells, whereas ER was expressed in scattered stromal cells and arterial cells. Both PR and ER were absent from glandular epithelium, contrasting with the secretory activity during the first trimester. Spiral arteries of the endometrium and myometrial smooth muscle cells showed intense PR and moderate ER staining in early pregnancy. The progesterone antagonist RU 38486 (mifepristone), given in early pregnancy at a dose of 200 mg, caused a marked increase in ER staining and a smaller increase in PR staining in stromal cells, whereas the glandular epithelium remained negative for both ER and PR (except for one and two specimens, respectively). We conclude the following. 1) Stromal cells retain PR despite the high progesterone levels during pregnancy, in keeping with the role of progesterone in stromal decidualization. The absence of PR from the secretory glandular epithelium suggests a paracrine link between decidualized stromal cells and epithelial cells. 2) Significant PR down-regulation by progesterone during pregnancy occurs only in epithelial cells of the endometrium. 3) In contrast, the absence or low level of ER staining in the various cell types of the endometrium during gestation concurs with the known effect (down-regulation) of steroid hormones on ER mRNA or protein levels. The increase in ER in human decidua after RU 38486 treatment indicates that the main cause of the low ER levels is progesterone secretion. 4) The intense PR staining in smooth muscle cells of spiral arteries during early pregnancy suggests that progesterone is essential for modulating blood flow during pregnancy.
authors.affiliation: Hormones et Reproduction, INSERM U135, Centre Hospitalo-Universitaire Kremlin-Bicétre, France.
authors.forename: M
authors.initials: M
authors.lastname: Perrot-Applanat
authors.forename: M
authors.initials: M
authors.lastname: Deng
authors.forename: H
authors.initials: H
authors.lastname: Fernandez
authors.forename: C
authors.initials: C
authors.lastname: Lelaidier
authors.forename: G
authors.initials: G
authors.lastname: Meduri
authors.forename: P
authors.initials: P
authors.lastname: Bouchard
mesh_terms: D000328:Adult; D001808:Blood Vessels / Q000378:metabolism; D004717:Endometrium / Q000098:blood supply*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015735:Mifepristone / Q000494:pharmacology; D011247:Pregnancy / Q000378:metabolism*; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D011959:Receptors, Estradiol / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D014018:Tissue Distribution; D014599:Uterus / Q000378:metabolism*
chemical_list: D011959:Receptors, Estradiol; D011980:Receptors, Progesterone; D015735:Mifepristone