title: Ultrastructure of calcified muscle fibres at the implantation site of demineralized bone matrix gelatin.
abstract: To clarify the mechanism of calcification during new bone formation induced by demineralized bone matrix gelatin (BMG), BMG was implanted into the skeletal muscle of Sprague-Dawley (SD) rats and histological changes were examined 1,2,3,5,7 and 10 days later. From day 2 after implantation, calcification was observed in muscle fibres near the implanted BMG. Calcification of the BMG ('acellular mineral deposition') was first seen on day 3 and in both, calcification increased. The calcification of muscle fibre was first seen in the sarcoplasmic reticulum (SR) and then in mitochondria or lipid droplet-like structures of satellite cells. The muscle mineral deposition occurred without osteogenic cells and matrix vesicles in the same way as 'acellular mineral deposition'.
authors.affiliation: Department of Anatomy, School of Dentistry, University of Tokushima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Takagi
mesh_terms: D000818:Animals; D000880:Anthraquinones; D001857:Bone Matrix / Q000502:physiology / Q000637:transplantation; D002113:Calcification, Physiologic / Q000187:drug effects / Q000502:physiology*; D005780:Gelatin; D008297:Male; D008854:Microscopy, Electron; D009132:Muscles / Q000648:ultrastructure*; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000880:Anthraquinones; C010078:alizarin; D005780:Gelatin