[Multiplicity of molecular forms of soluble carboxypeptidase-B-like enzymes in the cat brain].
Four forms of enzymes possessing carboxypeptidase-B-like activity have been detected in the soluble fraction of the cat encephalon. The first of these forms has molecular weight 50 kDa pH optimum is 5.0-5.6, it is four times activated by Co2+ ions and intensively inhibited by EDTA and reagents to sulphydryl groups. The other form has molecular weight 100 kDa, manifests maximum activity at pH 5.0-5.5 and is inhibited by HgCl2. EDTA, Co2+ ions and 2-mercaptoethanol take no effect on its activity. Molecular weight of the third form is not less than 280 kDa, optimum pH 5.5, is activated by Co2 ions and 2-mercaptoethanol, intensively inhibited by EDTA and reagents to sulfhydryl groups. The fourth form has molecular weight about 280 kDa, wide pH optimum with maximum at 5.5, is not activated by Co2+ ions and inhibited by EDTA, HgCl2, N-ethyl maleimide and 2-mercaptoethanol.
Vernigora
A N
AN
Nikihin
N N
NN
Gengin
M T
MT
D000818:Animals; D001921:Brain / Q000201:enzymology*; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000187:drug effects; D002415:Cats; D003035:Cobalt / Q000378:metabolism; D004492:Edetic Acid; D004789:Enzyme Activation; D005033:Ethylmaleimide; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000187:drug effects; D008623:Mercaptoethanol; D008627:Mercuric Chloride; D008970:Molecular Weight; D012995:Solubility; D013439:Sulfhydryl Reagents
D007527:Isoenzymes; D013439:Sulfhydryl Reagents; D003035:Cobalt; D008627:Mercuric Chloride; D008623:Mercaptoethanol; D004492:Edetic Acid; D002268:Carboxypeptidases; D043424:Carboxypeptidase B; D005033:Ethylmaleimide
Mnozhestvennost' molekuliarnykh form rastvorimykh karboksipeptidazo-B-podobnykh fermentov golovnogo mozga koshki.