title: Nonenzymatic versus enzymatic hepatocyte isolation from pig livers for larger scale investigations of liver cell perfusion systems.
abstract: A comparison of nonenzymatic and enzymatic hepatocyte isolation was performed on pig livers. The collagenase perfusion showed superior results: mean viability 72 +/- 10% versus a maximum viability of 21% using EDTA-perfusion. A five-step collagenase perfusion technique, developed for pig livers enables larger scale investigations, in order to develop methods for hepatocyte cultures in therapeutical liver cell perfusion systems.
authors.affiliation: Chirurgische Klinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.
authors.forename: J C
authors.initials: JC
authors.lastname: Gerlach
authors.forename: J
authors.initials: J
authors.lastname: Brombacher
authors.forename: J M
authors.initials: JM
authors.lastname: Courtney
authors.forename: P
authors.initials: P
authors.lastname: Neuhaus
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D004492:Edetic Acid; D008099:Liver / Q000166:cytology* / Q000502:physiology; D016031:Liver Transplantation; D008297:Male; D010477:Perfusion; D013552:Swine
chemical_list: D004492:Edetic Acid