Determination of acrylamide metabolite, mercapturic acid by high performance liquid chromatography.
A HPLC Assay was developed to identify and measure the metabolite of acrylamide, mercapturic acid, N-Acetyl-s-(propionamide)-cysteine (APC) in urine. O-phthalaldehyde (OPA) was utilized as a precolumn derivatizing agent in the assay. This derivative was isolated with a good selectivity by high performance liquid chromatography (HPLC) employing reversed phase ODS columns. The quantitation of the mercapturic acid derivative was reproducible and with a detection limit of 1 pmol. The average coefficient of variation for the runs carried out on the same day was approximately 4.6% at the range of 80-160 mumol.L-1 of APC, and the mean analytical recovery from urine samples was 94%. The metabolite of urine of workers exposed to acrylamide was identified as N-acetyl-s-(propionamide)-cysteine by HPLC chromatography and fluorescence scan and HPLC-Mass spectra. All results were identical with the authentic synthesized compound.
Wu
Y Q
YQ
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing.
Yu
A R
AR
Tang
X Y
XY
Zhang
J
J
Cui
T
T
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D020106:Acrylamide; D000178:Acrylamides / Q000652:urine*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D006801:Humans; D006868:Hydrolysis; D008297:Male; D016273:Occupational Exposure; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
D000178:Acrylamides; D020106:Acrylamide; C085597:N-acetyl-S-(propionamide)cysteine; D000111:Acetylcysteine