Modulation of the immunosuppressive activity of CKS-17, a synthetic retroviral envelope peptide, by muramyl dipeptide.
CKS-17, a heptadecapeptide corresponding to a region highly conserved in retroviral transmembrane proteins is known to be immunosuppressive both in vitro and in vivo when conjugated to a carrier protein. Here we examined the effect of the synthetic adjuvant muramyl dipeptide (MDP) on the immunosuppressive properties of CKS-17-BSA in vitro. MDP was found to abrogate CKS-17-BSA-induced inhibition of both IgM plaque-forming cell responses and antitetanus toxin IgG secretion by BALB/c mouse spleen cells immunized in vivo and in vitro by sheep red blood cells and tetanus toxoid, respectively. In contrast, the CKS-17-BSA suppression of concanavalin A-induced splenocyte proliferation was not abrogated by MDP. The data suggest that muramyl peptides could be useful as immunoadjuvants for vaccines against retrovirus-associated immunosuppressive diseases.
Gauduin
M C
MC
H. Lee Moffitt Cancer and Research Institute, University of South Florida College of Medicine, Tampa.
Vogel
F R
FR
Simon
J P
JP
Audibert
F A
FA
Chedid
L A
LA
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D015941:Complement Hemolytic Activity Assay; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007165:Immunosuppression Therapy; D036341:Intercellular Signaling Peptides and Proteins; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C053692:CKS 17