[General principles of the production and use of retroviral vectors].
This paper describes the various strategies for somatic gene therapy. There are two main scenarios: the ex vivo approach and the in vivo approach. As recombinant retroviruses were used in the first clinical trials of gene therapy, their main characteristics (structure, efficient production of helper-free viral stocks by using packaging cells, transduction procedures) are discussed as well as the related safety issues. Finally, retroviral vectors can also be used as a new tool to investigate various clinical situations as they allow to mark cells by stably integrating in the cellular genome.
Lehn
P
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Laboratoire de Biochimie Génétique, Université René Descartes, CHU Paris-Ouest, Hôpital Robert Debré, France.
D018014:Gene Transfer Techniques; D030342:Genetic Diseases, Inborn / Q000235:genetics / Q000628:therapy*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000628:therapy*; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics*
Principes généraux de la production et de l'utilisation des vecteurs rétroviraux.