title: [Metal resistance of gram-negative bacteria isolated from soil and waste waters of industrial regions].
abstract: Metals resistance of 112 strains of gram-negative bacteria isolated from soil and wastes around Nizniy Novgorod plants were investigated. MIC of metals varied in range between 1-10 mM Ni2+, Co2+, 1-6 mM Zn2+, 1-4 mM Cd2+, CrO4(2-), and 0.1-0.3 mM TeO3(2-). Bacteria with phenotypes NiCo and NiZnTe are revealed most frequent (25.6 and 6.4% accordingly). Plasmid DNAs with size between 2-70 kbp were found in 50% isolated strains. It was shown that resistance to cobalt of unidentified gram-negative bacterium is determined by plasmid genes. Recombinant plasmid contained fragment DNA controlling resistance to cadmium and zinc from Pseudomonas spp. strain was constructed by using helper plasmid pULB113.
authors.forename: L A
authors.initials: LA
authors.lastname: Anisimova
authors.forename: T V
authors.initials: TV
authors.lastname: Siunova
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D004352:Drug Resistance, Microbial / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D007220:Industrial Waste; D008670:Metals / Q000633:toxicity*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D012426:Russia; D012988:Soil Microbiology
chemical_list: D007220:Industrial Waste; D008670:Metals
vernacular_title: Ustoĭchivost' k metallam gramotritsatel'nykh bakteriĭ, izolirovannykh iz pochv i stochnykh vod promyshlennykh raĭonov.