Host-related DNA sequences are localized in the body of schistosome adults.
The localization of host (mouse)-related DNA sequences in the adults and cercariae of Schistosoma japonicum and S. mansoni was examined by in situ hybridization using 32P-labelled probes. The hybridization signals to the sequences of the mouse type C and type A retroviruses were clearly observed in the subtegumental layer and inner tissues of S. japonicum adults. In contrast, it was hard to find any signals to these sequences in S. mansoni adults. Distinct signals to the env-specific region of the mouse ecotropic type C retrovirus were observed in the subtegumental layer and inner tissues of S. mansoni adults. No signal to the sequence of the mouse type B retrovirus was found on the sections of either schistosome adult. The signals to the sequence of the genomic clone SmM51 derived from male S. mansoni were found in the tissues of both the schistosome adults. The signals to the sequences of the mouse type A and env-specific region of ecotropic type C retroviruses were not seen in the cercariae of each species, whereas the signals to the sequence of SmM51 were detected in S. mansoni cercariae. These observations suggest that host-related DNA sequences might be incorporated unequally into the schistosome genome during development in the final host.
Irie
Y
Y
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki-ken, Japan.
Iwamura
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Y
D000818:Animals; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015752:Genes, env; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008813:Mice, Inbred ICR; D009928:Organ Specificity; D012190:Retroviridae / Q000302:isolation & purification*; D012549:Schistosoma japonicum / Q000166:cytology / Q000235:genetics*; D012550:Schistosoma mansoni / Q000166:cytology / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral; D004247:DNA