title: Automated process monitoring of monoclonal antibody production.
abstract: Antifibronectin, monoclonal antibody was monitored through 52 h of production. Samples were automatically drawn from a bioreactor into the injection valve of an HPLC system without prior sample preparation. The hybridoma cell line was nonadherent, so whole cells were injected directly onto the perfusable protein A affinity column. There was only a modest column back pressure (ca. 1700 psi at a linear flow rate of 1.5 cm/s) after over 75 injections over the 52-h experiment. These experiments demonstrate the utility of high-speed chromatography for rapid process monitoring.
authors.affiliation: Department of Chemistry, Purdue University, Lafayette, Indiana 47907.
authors.forename: S K
authors.initials: SK
authors.lastname: Paliwal
authors.forename: T K
authors.initials: TK
authors.lastname: Nadler
authors.forename: D I
authors.initials: DI
authors.lastname: Wang
authors.forename: F E
authors.initials: FE
authors.lastname: Regnier
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D005353:Fibronectins / Q000276:immunology*; D006825:Hybridomas; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000096:biosynthesis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007074:Immunoglobulin G