The murine homologue of the human interleukin-8 receptor type B maps near the Ity-Lsh-Bcg disease resistance locus.
Interleukin-8 (IL-8), a member of the chemokine alpha subfamily, is a chemoattractant for neutrophils. Cell surface receptors for IL-8 have been cloned from rabbits and humans. Two related but different IL-8 receptors (IL-8R) have been characterized from humans. IL-8RA and IL-8RB bind IL-8 at high affinity but IL-8RB also binds GRO/MGSA and NAP-2 at high affinity. Using the human IL-8RB cDNA as a probe, we have determined that the homologous murine gene maps near the Ity-Lsh-Bcg disease resistance locus. A murine homologue of the human IL-8RB was isolated from a genomic library. This gene would encode a protein of 359 amino acids and would have a 68 and 71% amino acid identity with human IL-8RA and IL-8RB, respectively. Additional mapping data using the murine gene revealed the following genetic distances (in cM +/- 1 standard error) from the centromere: Mylf--7.9 +/- 2.7--Lsh-Ity-Bcg--1.9 +/- 1.4--Il8rb--1.9 +/- 1.4--Vil-- 5.9 +/- 2.3--Acrg--2.9 +/- 1.7--Bcl-2.
Cerretti
D P
DP
Immunex Corporation, Seattle, Washington 98101.
Nelson
N
N
Kozlosky
C J
CJ
Morrissey
P J
PJ
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Dosik
J K
JK
Mock
B A
BA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D018123:Receptors, Interleukin / Q000235:genetics* / Q000302:isolation & purification*; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B
D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D004247:DNA