Toxocara canis: failure to find IgE receptors (Fc epsilon R) on eosinophils from infected mice suggests that murine eosinophils do not kill helminth larvae by an IgE-dependent mechanism.
Eosinophils obtained by bronchoalveolar lavage (BAL) from the lungs of mice infected with Toxocara canis were characterized by flow cytometry with respect to cytophilic antibodies and surface Fc receptors. Freshly harvested BAL eosinophils were negative for sIgM, sIgA, sIgE, and Fc epsilon RII. These eosinophils were positive for sIgG1 and Fc gamma RII, although not all Fc gamma RIIs contained bound ligand. Culturing eosinophils for 24 or 48 hr with exogenous IgE and/or IL-4 did not induce IgE binding capacity or Fc epsilon RII expression. IL-4 did not decrease Fc gamma RII expression but did decrease ligand binding capacity by Fc gamma RII. These findings are in marked contrast to the results of studies characterizing the surface of both human and rat eosinophils and may indicate different functional activities for mouse BAL eosinophils in helminth infections.
Jones
R E
RE
Department of Structural and Cellular Biology, College of Medicine, University of South Alabama, Mobile 36688.
Finkelman
F D
FD
Hester
R B
RB
Kayes
S G
SG
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002478:Cells, Cultured; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007814:Larva / Q000276:immunology; D008168:Lung / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D017455:Receptors, IgE / Q000032:analysis*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D017166:Toxocara canis / Q000276:immunology*; D014120:Toxocariasis / Q000276:immunology*
D007074:Immunoglobulin G; D017455:Receptors, IgE; D007073:Immunoglobulin E