title: Molecular cloning of a murine N-type calcium channel alpha 1 subunit. Evidence for isoforms, brain distribution, and chromosomal localization.
abstract: A cDNA encoding a N-type Ca2+ channel has been cloned from the murine neuroblastoma cell line N1A103. The open reading frame encodes a protein of 2,289 amino acids (257 kDa). Analysis of different clones provided evidence for the existence of distinct isoforms of N-type channels. High levels of mRNA were found in the pyramidal cell layers CA1, CA2 and CA3 of the hippocampus, in the dentate gyrus, in the cortex layers 2 and 4, in the subiculum and the habenula. The N-type Ca2+ channel gene has been localized on the chromosome 2, band A.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
authors.forename: T
authors.initials: T
authors.lastname: Coppola
authors.forename: R
authors.initials: R
authors.lastname: Waldmann
authors.forename: M
authors.initials: M
authors.lastname: Borsotto
authors.forename: C
authors.initials: C
authors.lastname: Heurteaux
authors.forename: G
authors.initials: G
authors.lastname: Romey
authors.forename: M G
authors.initials: MG
authors.lastname: Mattéi
authors.forename: M
authors.initials: M
authors.lastname: Lazdunski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015698:Genomic Library; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D015220:Calcium Channels; D018076:DNA, Complementary; D012333:RNA, Messenger