Isolation of the cDNA and chromosomal localization of the gene (TAX1) encoding the human axonal glycoprotein TAG-1.
The transient axonal glycoprotein (TAG-1) is a cell adhesion molecule that promotes neurite outgrowth and belongs to the immunoglobulin superfamily. We have isolated cDNAs encoding TAX1, the human homologue of TAG-1. Human TAX1 shows a high degree of homology to rat TAX1 and less to its chick counterpart, axonin-1, with 91 and 75% identity at the amino acid level, respectively. The numbers of immunoglobulin (IgC2) domains and fibronectin repeats present in TAG-1 are conserved among the three species. The highest degree of conservation occurs in the second IgC2 domain (98% with the rat and 82% with the chick). The human homologue also contains a putative N-terminal signal sequence and a C-terminal hydrophobic sequence, suggestive of linkage to the cell membrane via phosphatidylinositol. In addition, the two mammalian TAG-1 proteins share the RGD tripeptide, a motif known to mediate recognition of fibronectin by integrins. In situ hybridization to human metaphase chromosomes maps the TAX1 gene encoding human TAG-1 to a single location on chromosome 1q32.
Tsiotra
P C
PC
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Crete, Greece.
Karagogeos
D
D
Theodorakis
K
K
Michaelidis
T M
TM
Modi
W S
WS
Furley
A J
AJ
Jessell
T M
TM
Papamatheakis
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015816:Cell Adhesion Molecules, Neuronal; D002531:Cerebellum / Q000378:metabolism*; D002645:Chickens / Q000235:genetics; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D017124:Conserved Sequence; D058969:Contactin 2; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification*; D005353:Fibronectins / Q000235:genetics; D005803:Genes, Immunoglobulin; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats / Q000235:genetics; D017386:Sequence Homology, Amino Acid
C548674:CNTN2 protein, human; D015816:Cell Adhesion Molecules, Neuronal; C548676:Cntn2 protein, rat; D058969:Contactin 2; D017931:DNA Primers; D018076:DNA, Complementary; D005353:Fibronectins; D008562:Membrane Glycoproteins