title: Culture medium composition affects the relative toxicities of chlorobenzenes in rat liver slices and the isolated perfused liver.
abstract: The effects of different media composition on the hepatotoxicity produced by monochlorobenzene (MCB), 1,2-dichlorobenzene (1,2-DCB), 1,3-dichlorobenzene (1,3-DCB) and 1,4-dichlorobenzene (1,4-DCB) were examined in two different in vitro systems. The toxicity of these chlorobenzenes was investigated in the perfused rat liver and liver slices using Krebs-Henseleit buffer. Significant differences between the chlorobenzenes were apparent in the perfused liver but not in the tissue slices. However, a dose and time related response of rat liver slices to the chlorobenzenes was observed. Partial amelioration of the chlorobenzene toxicity was observed when the Krebs-Henseleit buffer was supplemented with vitamins, amino acids, and/or bovine serum albumin. 1,2-DCB and 1,3-DCB toxicity was affected by amino acids and vitamins. The toxicity produced by 1,4-DCB was suppressed by amino acids, vitamins and 1% BSA. MCB hepatoxicity could only be suppressed by 1% BSA. This data suggests that tissue culture media composition plays a major role in the hepatotoxicity of the chlorobenzenes.
authors.affiliation: University of Arizona, Department of Pharmacology, Tucson.
authors.forename: R L
authors.initials: RL
authors.lastname: Fisher
authors.forename: A J
authors.initials: AJ
authors.lastname: Gandolfi
authors.forename: I G
authors.initials: IG
authors.lastname: Sipes
authors.forename: K
authors.initials: K
authors.lastname: Brendel
mesh_terms: D000818:Animals; D002722:Chlorobenzenes / Q000633:toxicity*; D003470:Culture Media; D046508:Culture Techniques; D008099:Liver / Q000187:drug effects*; D008297:Male; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002722:Chlorobenzenes; D003470:Culture Media; C004726:2-dichlorobenzene; C042146:1,3-dichlorobenzene; C018511:4-dichlorobenzene; C031294:chlorobenzene