title: Bis(dithiocarbamato) nitrido technetium-99m radiopharmaceuticals: a class of neutral myocardial imaging agents.
abstract: The synthesis and biodistribution in various animal models (rat, dog, pig and monkey) of 99mTc radiopharmaceuticals containing the Tc = N multiple bond are reported.
  
  
  METHODS
  The complexes are represented by the general formula 99mTcN(L)2, where L is the monoanionic form of a dithiocarbamate ligand of the type [R1(R2)-N-C(=S)S]-, and R1 and R2 are variable, lateral groups. The preparations were carried out, both as a liquid and freeze-dried formulation, through a simple procedure involving the initial reaction of [99mTcO4]- with S-methyl N-methyl dithiocarbazate [H2NN(CH3)C(=S)SCH3], in the presence of tertiary phosphines or Sn2+ ion as reductants, followed by the addition of the sodium salt of the ligand (NaL) to afford the final product. The chemical identity of the resulting complexes was determined by comparing their chromatographic properties with those of the corresponding 99Tc analogs characterized by spectroscopic and x-ray crystallographic methods. The complexes are neutral and possess a distorted, square pyramidal geometry.
  
  
  RESULTS
  No decomposition of the complexes, in physiological solution, was observed over a period of 6 hr. Imaging and biodistribution studies demonstrated that these radiopharmaceuticals localize selectively in the myocardium of rats, dogs and primates, but that they failed to visualize the pig heart. The kinetics of heart uptake and clearance were studied in rats and dogs, and found to be strongly influenced by variation of the lateral R1 and R2 groups.
  
  
  CONCLUSIONS
  The high quality of myocardial images obtained in dogs and monkeys demonstrates that the derivative 99mTcN[E-t(EtO)NCS2]2 [99mTcN(NOEt)] exhibits the most favorable distribution properties for further studies in humans.
authors.affiliation: CIS Bio International, Gif-sur-Yvette, France.
authors.forename: R
authors.initials: R
authors.lastname: Pasqualini
authors.forename: A
authors.initials: A
authors.lastname: Duatti
authors.forename: E
authors.initials: E
authors.lastname: Bellande
authors.forename: V
authors.initials: V
authors.lastname: Comazzi
authors.forename: V
authors.initials: V
authors.lastname: Brucato
authors.forename: D
authors.initials: D
authors.lastname: Hoffschir
authors.forename: D
authors.initials: D
authors.lastname: Fagret
authors.forename: M
authors.initials: M
authors.lastname: Comet
mesh_terms: D000818:Animals; D004285:Dogs; D006321:Heart / Q000000981:diagnostic imaging*; D008252:Macaca fascicularis; D008297:Male; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D013552:Swine; D017556:Technetium Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D017556:Technetium Compounds