Expression of interleukin 1 family mRNAs by a highly branched (1-->3)-beta-D-glucan, OL-2.
OL-2, a highly branched (1-->3)-beta-D-glucan, is an antitumor glucan showing strong hematopoietic activity with weaker adjuvant activity than schizophyllan (SPG), also an antitumor glucan and one which is clinically used. This paper deals with the gene expression of the interleukin 1 (IL-1) family in mice by OL-2 and SPG in order to characterize the immunopharmacological activity. Gene expression was examined by reverse transcriptase-polymerase chain reaction method. Intraperitoneal administration of OL-2 (250 micrograms/mouse) expressed all three genes of IL-1 alpha, beta, and IL-1 receptor antagonist (IL-1ra) in the peritoneal exudate cells, while SPG induced a strength of IL-1 alpha mRNA comparable to that by OL-2 but a weaker level of IL-1 beta mRNA. SPG did not induce IL-1ra. Similar patterns were seen in spleen and liver by OL-2 or SPG administration. These findings suggest that the immunopharmacological characteristics of (1-->3)-beta-D-glucan are regulated under the gene expression of the IL-1 family.
Nemoto
J
J
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
Ohno
N
N
Saito
K
K
Adachi
Y
Y
Yadomae
T
T
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D015870:Gene Expression / Q000187:drug effects*; D005936:Glucans / Q000494:pharmacology*; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012795:Sialoglycoproteins / Q000235:genetics*
D000970:Antineoplastic Agents; D005936:Glucans; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C074376:OL 2