Promoter analysis of the bli-7/eas gene.
Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between -1498 bp and -1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between -595 bp and -429 bp, as well as a stretch of DNA from -429 bp to -380 bp which may exert a positive influence after light induction.
Kaldenhoff
R
R
Universität Hannover, Institut für Botanik, Germany.
Russo
V E
VE
D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D008027:Light; D009492:Neurospora crassa / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D014170:Transformation, Genetic
D005656:Fungal Proteins; D005947:Glucose; D009584:Nitrogen