Analysis of an insulin gene transcription control element. Positive and negative regulation appears to be mediated by different element sequences.
Pancreatic beta-cell-type-specific transcription of the insulin gene is controlled by cis-acting sequence elements lying within its enhancer region. An essential element required for expression is the insulin control element (ICE). The activity of this element is regulated by both positive- and negative-acting transcription factors. In this study, we have identified the nucleotide sequences within the ICE that are required for repression in noninsulin producing cells. Our results indicate that the cis-acting sequences involved in negative control are distinct from those required in activating expression in beta cells.
Stein
R
R
Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232.
Henderson
E
E
Cordle
S R
SR
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D006224:Cricetinae; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007328:Insulin / Q000235:genetics*; D007668:Kidney; D008969:Molecular Sequence Data; D017354:Point Mutation; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins; D014158:Transcription, Genetic
D007328:Insulin; D012097:Repressor Proteins