Fluorescent in situ hybridization (FISH): a new application in the delineation of true vs. pseudomosaicism in prenatal diagnosis.
Metaphase chromosomes and interphase nuclei from nine amniotic fluid cultures were studied with fluorescence in situ hybridization (FISH). The samples were initially analyzed with routine G-banding and were diagnosed as having true mosaicism (five patients) or pseudomosaicism (four patients). In our study, FISH analysis could provide additional information to distinguish pseudo- from true mosaicism by allowing interphase studies and analysis of an increased number of metaphase spreads. These results suggest a multilinear origin of 'in situ' colonies of cells.
Schwartz
S
S
Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore.
Leana-Cox
J
J
D000653:Amniotic Fluid / Q000502:physiology; D001399:Azure Stains; D002871:Chromosome Banding; D015342:DNA Probes; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase / Q000235:genetics; D009030:Mosaicism; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*
D001399:Azure Stains; D015342:DNA Probes