title: Increased vulnerability to neuronal damage after umbilical cord occlusion in fetal sheep with advancing gestation.
abstract: OBJECTIVE
  The purpose of this study was to compare systemic responses and neurologic consequences of umbilical cord occlusion in fetal sheep with advancing gestation.
  
  
  METHODS
  The umbilical cord was occluded for 10 minutes in nine midgestation (90 to 92 days) and 10 late-gestation (135 to 136 days) chronically instrumented fetuses. Systemic and cortical electrophysiologic effects were compared by analysis of variance. The extent of neuronal loss was determined 3 days later.
  
  
  RESULTS
  During occlusion, hypotension (23 +/- 2 mm Hg) and cortical cytotoxic edema were more marked in older fetuses (p < 0.001). On reperfusion, the edema, rebound tachycardia, and hypertension resolved within 20 minutes. Recovery of electroencephalographic activity (3.1 +/- 0.8 hours) and lactate levels (> 2 hours) was slower in late-gestation fetuses (p < 0.05). Neuronal loss, which was observed only in the older group, was predominantly in the hippocampus and was associated with the severity of hypotension during umbilical occlusion but not with systemic lactate levels.
  
  
  CONCLUSIONS
  Late-gestation fetal sheep are neurologically more susceptible to umbilical cord occlusion than are midgestation fetal sheep. Possibly the lesser ability of the older fetuses to maintain blood pressure and cerebral plasma membrane function during asphyxia contributes to the greater vulnerability in the gray matter.
authors.affiliation: Research Centre for Developmental Medicine and Biology, Auckland, New Zealand.
authors.forename: E C
authors.initials: EC
authors.lastname: Mallard
authors.forename: C E
authors.initials: CE
authors.lastname: Williams
authors.forename: B M
authors.initials: BM
authors.lastname: Johnston
authors.forename: P D
authors.initials: PD
authors.lastname: Gluckman
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D001921:Brain / Q000098:blood supply; D001929:Brain Edema / Q000175:diagnosis / Q000209:etiology; D002245:Carbon Dioxide / Q000097:blood; D003250:Constriction; D004195:Disease Models, Animal; D004569:Electroencephalography; D004594:Electrophysiology; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D005311:Fetal Hypoxia / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D005865:Gestational Age; D006339:Heart Rate; D007022:Hypotension / Q000209:etiology; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D009420:Nervous System / Q000473:pathology*; D010100:Oxygen / Q000097:blood; D011247:Pregnancy; D012756:Sheep; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007773:Lactates; D002245:Carbon Dioxide; D019344:Lactic Acid; D010100:Oxygen