The 1629-bp open reading frame of the Autographa californica multinucleocapsid nuclear polyhedrosis virus encodes a virion structural protein.
A 1629-bp open reading frame (ORF) of Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) is shown to encode a 78-kDa virion structural protein. To determine this, polyclonal antibody was made to a fusion protein synthesized in Escherichia coli from a chimeric gene that contained 1415 bp of the 1629-bp gene. In Western blot analyses, this antibody cross-reacted with a protein of about 78 kDa in both extracellular virions (ECV) and virions isolated from polyhedra (PDV), and with a 78-kDa protein in PDV envelope preparations, but not with PDV nucleocapsids. This suggests that the protein encoded by the 1629-bp ORF is a virion envelope protein or a protein that occurs in the virion intermediate layer between the envelope and nucleocapsid.
Pham
D Q
DQ
Department of Entomology, University of California, Riverside 92521.
Hice
R H
RH
Sivasubramanian
N
N
Federici
B A
BA
D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D003001:Cloning, Molecular; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames; D015678:Viral Structural Proteins / Q000235:genetics* / Q000276:immunology; D014771:Virion / Q000235:genetics
D000914:Antibodies, Viral; D015678:Viral Structural Proteins