Up-regulation of lipocortin-1 and its mRNA in reactive astrocytes in kainate-lesioned rat cerebellum.
We have used a combined molecular and immunocytochemical approach to examine the expression of lipocortin-1 (LC-1) in kainate-lesioned rat cerebellum. Using immunocytochemistry, Western and Northern blotting, we have shown upregulation of LC-1 mRNA and expression of LC-1 localised specifically to reactive astrocytes. These studies suggest that reactive astrocytes are a major synthetic compartment for the expression of LC-1. The well-reported immuno-suppressive effects of lipocortin(s), suggests that reactive astrocytes could serve to negatively modulate inflammatory reactions in the central nervous system.
Mullens
L
L
Department of Medicine, Charing Cross & Westminster Medical School, London, UK.
Marriott
D R
DR
Young
K A
KA
Tannahill
L
L
Lightman
S L
SL
Wilkin
G P
GP
D000818:Animals; D017305:Annexin A1 / Q000032:analysis* / Q000235:genetics; D001253:Astrocytes / Q000737:chemistry*; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D002531:Cerebellum / Q000737:chemistry*; D007150:Immunohistochemistry; D007608:Kainic Acid; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
D017305:Annexin A1; D012333:RNA, Messenger; D007608:Kainic Acid