Epidermis as a secretory tissue: an in vitro tissue model to study keratinocyte secretion.
In addition to protective functions, keratinocytes secrete a variety of effector molecules that may have local or distant effects. To explore the secretory activity of keratinocytes we have developed a two-chamber culture model in which a fully differentiated stratified epithelium is present in the upper chamber and secreted protein is collected in a lower chamber. A collagen matrix is not used and during collection of secreted protein, 3T3 feeder cells are absent. Keratinocytes secrete protein at the rate of 0.67 microgram/h/10(6) cells, encompassing a molecular weight range from several kD to over 180 kD. Secretion is an unexplored area of keratinocyte biology and this model will allow investigation of this activity.
Katz
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Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook 11794-8702.
Taichman
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D000328:Adult; D015153:Blotting, Western; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D004702:Endocrine Glands / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008954:Models, Biological; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis / Q000378:metabolism
D003470:Culture Media; D011506:Proteins