Non-isotopic in situ detection of mRNA for interleukin-4 in archival human tissue.
Non-isotopic in situ hybridisation (NISH) is a rapid and sensitive method currently used in molecular pathology to identify DNA in fresh and archival human biopsies. In this study we have extended NISH technology for mRNA detection to include the use of digoxigenin-labelled riboprobes to localise low abundance mRNA for interleukin-4 in paraffin embedded material (from active inflammatory bowel disease). As this methodological approach makes the retrospective study of cytokine gene expression in archival material possible for the first time, it could act as a prototype for studies of cytokine involvement in various human diseases.
Bromley
L
L
Nuffield Department of Pathology and Bacteriology, University of Oxford, John Radcliffe Hospital, Headington, UK.
McCarthy
S P
SP
Stickland
J E
JE
Lewis
C E
CE
McGee
J O
JO
D003093:Colitis, Ulcerative / Q000276:immunology; D004076:Digoxigenin; D016591:Histocytological Preparation Techniques; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D015847:Interleukin-4 / Q000235:genetics*; D007422:Intestines / Q000737:chemistry; D015347:RNA Probes; D012333:RNA, Messenger / Q000302:isolation & purification*
D015347:RNA Probes; D012333:RNA, Messenger; D015847:Interleukin-4; D004076:Digoxigenin