Purification of a capillary permeability-increasing enzyme-2 from the venom of Agkistrodon caliginosus (Kankoku-Mamushi).
A capillary permeability-increasing enzyme-2 was purified from the venom of A. caliginosus by ion-exchange chromatography and gel filtration on Sephadex G-100. By this procedure, 3.1 mg of purified enzyme was obtained from 4 g of the venom. The mol. wt of the purified enzyme was estimated to be approximately 44,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme hydrolyzed N-alpha-tosyl-L-arginine methylester with a specific activity of 56.5 units/mg of protein, and did not show any caseinolytic, clotting or bradykinin-releasing activity. When 13.9 micrograms of the enzyme was injected into the depilated skin on the back of a rabbit, capillary permeability was increased.
Shimokawa
K
K
Division of Chemistry of Hygiene, Meiji College of Pharmacy, Tokyo, Japan.
Takahashi
H
H
D017836:Agkistrodon; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D002417:Cattle; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000201:enzymology*; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D007705:Kinins / Q000378:metabolism; D051381:Rats; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000494:pharmacology
C031442:Agkistrodon venoms; D003435:Crotalid Venoms; D007705:Kinins; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D012697:Serine Endopeptidases; C085657:capillary permeability-increasing enzyme 2