Localization of neurotrophin receptors in olfactory epithelium and bulb.
We used in situ hybridization to localize trk, trkB and trkC mRNA, in rat and cat olfactory bulb. Expression of mRNA encoding truncated trkB receptors was seen in all layers, while only very modest full-length trkB expression could be detected. trkC hybridization was seen in all layers, most dense in the mitral cell layer. The localization of full-length tyrosine kinase trkB receptor in olfactory bulb and epithelium was examined with immunohistochemistry. trkB-like immunoreactivity was seen in the fila olfactoria, epithelium and in vitro, in olfactory sensory neurones. Since BDNF is expressed by olfactory sensory neurone target cells in the olfactory bulb, these data suggest that BDNF may act as a target derived neurotrophic factor in the primary olfactory system.
Deckner
M L
ML
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Frisén
J
J
Verge
V M
VM
Hökfelt
T
T
Risling
M
M
D000818:Animals; D002415:Cats; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009830:Olfactory Bulb / Q000033:anatomy & histology / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000033:anatomy & histology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*
D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor