Effect of ketamine-anesthesia on N-methyl-D-aspartate-induced activation of type I nucleus accumbens neurons.
Extracellular single-unit recording and microiontophoretic techniques were used to determine the effects of ketamine-anesthesia on N-methyl-D-aspartate (NMDA)-induced excitation of Type I nucleus accumbens neurons. NMDA increased the firing rate of most neurons in this preparation. Thus, it may be concluded that ketamine-anesthesia does not result in blockade of central NMDA receptors. The excitation caused by NMDA was readily reversed in all cases by co-iontophoresis of MK 801, but was generally unaffected by coiontophoresis of ketamine. However, ketamine-anesthesia did significantly increase the current levels necessary for, and limited the magnitude of, NMDA-induced activation of these cells (as compared to urethane-anesthetized rats), suggesting that ketamine is not without effect on NMDA-receptors in vivo.
Kelland
M D
MD
Department of Psychiatry, Wayne State University School of Medicine, Detroit, MI 48201.
Chiodo
L A
LA
D000758:Anesthesia; D000818:Animals; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004594:Electrophysiology; D007478:Iontophoresis; D007649:Ketamine; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016202:N-Methylaspartate; D007649:Ketamine; D016291:Dizocilpine Maleate