[Detection of HIV-1 proviral DNA in peripheral leukocytes by AMPLICOR HIV-1 test kit].
AMPLICOR HIV-1 test kits, which had been developed as an HIV-1 provirus detection test by PCR method, have been evaluated for its clinical diagnostic application. Sixty-six of HIV-1 antibody positive and 67 of HIV-1 antibody negative blood samples derived from hemophiliacs, who had received blood products, have been tested by AMPLICOR HIV-1. All of the results from AMPLICOR HIV-1 were consistent with those from antibody test and clinical aspects. Thirty-nine of HIV-1 antibody positive samples have been tested by AMPLICOR HIV-1 and virus isolation (culture method). Twelve of 39 (30.8%) were positive by virus isolation, and 39 of 39 (100%) were positive by AMPLICOR HIV-1. Two of new born infants from HIV-1 sero-positive mothers were tested by AMPLICOR HIV-1, and the result suggested that the kit would be useful for diagnosis of infants from sero-positive mothers. Based on these studies, AMPLICOR HIV-1 is considered as useful clinical diagnostic for HIV-1 proviral DNA detection.
Yoshihara
N
N
AIDS Research Center, National Institute of Health, Shinjuku-ku, Tokyo.
Fukushima
K
K
Suzuki
H
H
Honda
M
M
Yamazaki
S
S
Fukutake
K
K
Meguro
T
T
Yamada
K
K
D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005260:Female; D015658:HIV Infections / Q000175:diagnosis; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007231:Infant, Newborn; D007962:Leukocytes / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic