Detection of anti-helminth antibody by microenzyme-linked immunosorbent assay using recombinant antigen and anti-beta-galactosidase monoclonal antibody.
An enzyme-linked immunosorbent assay (ELISA) using antigenic beta-galactosidase-helminth derived recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human helminthiasis. Helminth-infected human sera reacted strongly with FP that was immobilized with anti-beta-galactosidase monoclonal antibody on microplates. However, FP did not react with sera from patients with other helminthiases. In detection of anti-helminth IgG antibody, the present ELISA system using FP and anti-beta-galactosidase monoclonal antibody was highly specific compared to that using biochemically extracted soluble antigens.
Sugane
K
K
Department of Parasitology, Shinshu University School of Medicine, Nagano prefecture, Japan.
Sun
S H
SH
D000909:Antibodies, Helminth / Q000097:blood*; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006373:Helminthiasis / Q000175:diagnosis*; D006801:Humans; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D013045:Species Specificity; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D011994:Recombinant Proteins; D001616:beta-Galactosidase