Identification and cloning of a cyclic beta-(1-->3), beta-(1-->6)-D-glucan synthesis locus from Bradyrhizobium japonicum.
A cosmid clone complementing a cyclic beta-(1-->2)-glucan biosynthesis (ndvB) mutant of Rhizobium meliloti was isolated from a Bradyrhizobium japonicum gene library. This clone specified synthesis of beta-(1-->3), beta-(1-->6)-linked glucans in R. meliloti. The complemented strain was osmotically tolerant and symbiotically competent on alfalfa. Thus, beta-(1-->3), beta-(1-->6)-glucans can substitute functionally for beta-(1-->2)-glucans in R. meliloti.
Bhagwat
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AA
Soybean and Alfalfa Research Laboratory, USDA, Agricultural Research Service, Beltsville, Maryland 20705.
Tully
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RE
Keister
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DL
D003001:Cloning, Molecular / Q000379:methods*; D015723:Gene Library; D005798:Genes, Bacterial; D005936:Glucans / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D009586:Nitrogen Fixation / Q000235:genetics; D009994:Osmolar Concentration; D011994:Recombinant Proteins / Q000096:biosynthesis; D012230:Rhizobiaceae / Q000235:genetics*; D016962:Sinorhizobium meliloti / Q000235:genetics*
D005936:Glucans; D011994:Recombinant Proteins; C033671:epiglucan