Secondary thrombin-binding site, glycosaminoglycan binding domain and reactive center region of leuserpin-2 are strongly conserved in mammalian species.
Using a combination of conventional and inverse polymerase chain reaction the cDNA structure coding for the thrombin inhibitor leuserpin-2 from rat has been determined. The rat genome specifies leuserpin-2 mRNAs differing in their 5'-untranslated regions probably due to alternative splicing of the primary transcript. Comparisons of partial sequences from five different mammalian species show that the secondary thrombin binding site, the glycosaminoglycan binding domain and the reactive center region of the inhibitor are strongly conserved.
Westrup
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D
Hoechst AG, Frankfurt, Germany.
Ragg
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H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018076:DNA, Complementary / Q000737:chemistry; D004285:Dogs; D006025:Glycosaminoglycans / Q000378:metabolism*; D015844:Heparin Cofactor II / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013917:Thrombin / Q000378:metabolism*
D018076:DNA, Complementary; D006025:Glycosaminoglycans; C047932:SERPIND1 protein, human; D015844:Heparin Cofactor II; D013917:Thrombin