title: Detection of established and novel alleles of the HLA-DPB1 locus by PCR-SSO.
abstract: The polymorphism at the HLA-DPB1 locus was investigated in 490 Austrian individuals. Exon 2 sequences of the HLA-DPB1 genes were amplified by polymerase chain reaction and probed with a panel of sequence-specific oligonucleotide probes. HLA-DPB1 genotypes were deduced from the hybridization pattern of the probes. HLA-DPB1 allele frequencies and Hardy-Weinberg equilibrium were calculated. Linkage disequilibria between HLA-DPB1 and HLA-DQA1 alleles were determined. Four novel hybridization patterns indicating new alleles were found.
authors.affiliation: Clinical Department for Blood Group Serology, University of Vienna, Austria.
authors.forename: W F
authors.initials: WF
authors.lastname: Pickl
authors.forename: I
authors.initials: I
authors.lastname: Faé
authors.forename: G F
authors.initials: GF
authors.lastname: Fischer
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D005787:Gene Frequency; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D006801:Humans; D015810:Linkage Disequilibrium; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction
chemical_list: D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D009841:Oligonucleotides