Creatinine assay by the fuller's earth procedure or by enzymatic determination is adequate for urine but not plasma of mice.
1. Mouse plasma creatinine measured by fuller's earth (15.1 +/- 0.5(41) microM, mean +/- SE) exceeded the same pools read by HPLC [7.5 +/- 0.2(41); P < 0.001]. 2. For mouse urine, the fuller's earth method (3.26 +/- 0.21 mM) slightly exceeded the HPLC method (2.65 +/- 0.18; P < 0.001) with good correlation (r = 0.915, P < 0.001). 3. An enzymatic method (creatinine PAP, Boehringer-Mannheim Diagnostics) for plasma creatinine (5.2 +/- 0.3 microM) was equivalent to HPLC (6.1 +/- 0.1, NS) but with poor correlation (r = 0.23, NS). 4. Creatinine in the urine agreed closely between HPLC (2.84 +/- 0.12 mM) and the enzymatic procedure (2.95 +/- 0.17, NS) with good correlation (r = 0.845, P < 0.001).
Meyer
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Baxter Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232-2861.
Smith
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Meyer
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D017607:Aluminum Compounds; D000581:Amidohydrolases; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000032:analysis* / Q000097:blood / Q000652:urine; D005260:Female; D017616:Magnesium Compounds; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008817:Mice, Mutant Strains; D010089:Oxidoreductases, N-Demethylating; D050879:Sarcosine Oxidase; D017640:Silicates; D014512:Ureohydrolases
D017607:Aluminum Compounds; D017616:Magnesium Compounds; D017640:Silicates; C001262:Fuller's Earth; D003404:Creatinine; D010089:Oxidoreductases, N-Demethylating; C494350:Pipox protein, mouse; D050879:Sarcosine Oxidase; D000581:Amidohydrolases; C019946:creatininase; D014512:Ureohydrolases; C019944:creatinase