Determination of phenylmercapturic acid in urine of benzene-exposed BDF-1 mice.
This paper describes a procedure for the identification of phenylmercapturic acid in urine of benzene-exposed mice. Collected urine of benzene exposed mice was adjusted to pH 7 and applied to an anion exchanger. After extraction with diethyl ether and evaporation to dryness, the sample was dissolved in aqueous phosphoric acid and injected into the HPLC. HPLC conditions included an ODS column and an eluent consisting of tetrabutylammoniumhydrogensulfate-methanol (75:25, v/v), the absorbance wavelength was 255 nm. The detection limit of phenylmercapturic acid was 3 mg/l in mouse urine.
Schäfer
F
F
Institute of Occupational and Social Medicine, University of Ulm, Germany.
Schad
H
H
Weber
L
L
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D000818:Animals; D001554:Benzene / Q000378:metabolism* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D005260:Female; D051379:Mice; D013056:Spectrophotometry, Ultraviolet
C053003:S-phenyl-N-acetylcysteine; D001554:Benzene; D000111:Acetylcysteine