title: Adherence of haemagglutinating Helicobacter pylori to five cell lines.
abstract: Helicobacter pylori causes gastritis and is an important factor for the development of peptic ulcer disease in man. We used two different methods to examine the adhesion of nine H. pylori strains, with different haemagglutinating properties, to five cell lines, HeLa S3, HFI, Vero, SW1222 and WEHI cells. The adhesion studies were performed a) as bacterial adhesion to a monolayer of tissue culture cells, visualizing bacteria with fluorescein-isothiocyanate-labelled antibodies, b) as cell agglutination with bacteria and eucaryotic cells mixed in a suspension. The H. pylori strains were divided into three groups according to their cell adhesion properties. In general, H. pylori strains which showed the best adhesion to the five cell lines were strains which showed the best capability of agglutinating erythrocytes of several animal species. It is likely that the same adhesins are involved in cell adhesion and in haemagglutination. The two methods gave similar results.
authors.affiliation: Department of Medical Microbiology, University of Lund, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Ringnér
authors.forename: P
authors.initials: P
authors.lastname: Aleljung
authors.forename: T
authors.initials: T
authors.lastname: Wadström
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D002522:Chlorocebus aethiops; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006386:Hemagglutination Tests; D006801:Humans; D051379:Mice; D014709:Vero Cells