Randomly amplified polymorphic DNAs assess recombination following an induced parasexual cycle in Penicillium roqueforti.
Random amplified polymorphic DNAs (RAPDs) were used as a genetic marker system to characterize recombinant strains following the parasexual cycle of Penicillium roqueforti. After protoplast fusion and haploidization of diploid hybrids, segregants characterized by a reassortment of the parental genetic markers displayed specific RAPD fingerprints. The appearance or the loss of RAPD fragments demonstrate that these markers provide an efficient method to analyze recombination and to characterize somatic hybrids.
Durand
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Laboratoire de Biologie Cellulaire Fongique, UMR CNRS 106, Université Lyon I (Bât. 405), Villeurbanne, France.
Reymond
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Fèvre
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D001483:Base Sequence; D004271:DNA, Fungal; D005819:Genetic Markers; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010407:Penicillium / Q000235:genetics* / Q000254:growth & development; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic
D004271:DNA, Fungal; D005819:Genetic Markers