title: Serum antibody responses against shigella lipopolysaccharides and invasion plasmid-coded antigens in shigella infected Swedish patients.
abstract: Serum antibody responses to shigella lipopolysaccharide (LPS) and invasion plasmid-coded antigens (Ipa) were studied in 74 Swedish patients with culture verified bacillary dysentery using class-specific enzyme immunoassay (EIA). Anti-LPS responses were found in 80% and 79% serum samples, respectively, from S. flexneri and S. sonnei infected patients and anti-Ipa responses in 60% and 43%, respectively. The mean anti-Ipa IgG antibody titres in S. flexneri infected patients remained high for 4-6 months after onset while the anti-LPS IgG antibody titres had dropped to normal levels. The specificity of EIA for shigella Ipa was 90% and for S. dysenteriae, S. flexneri and S. sonnei LPSs it varied between 84% and 90%. No close correlations between the anti-LPS and anti-Ipa antibody responses were observed indicating that they may be differently regulated. The dynamics of the serum antibody responses indicates that an anti-LPS response is a good indicator of a recent shigella infection and an anti-Ipa IgG response a good indicator of a previous infection.
authors.affiliation: Karolinska Institute, Department of Clinical Bacteriology, Huddinge Hospital, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Li
authors.forename: Z C
authors.initials: ZC
authors.lastname: Rong
authors.forename: E
authors.initials: E
authors.lastname: Ekwall
authors.forename: U
authors.initials: U
authors.lastname: Forsum
authors.forename: A A
authors.initials: AA
authors.lastname: Lindberg
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins; D004405:Dysentery, Bacillary / Q000097:blood / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology*; D012680:Sensitivity and Specificity; D012762:Shigella dysenteriae / Q000276:immunology*; D012763:Shigella flexneri / Q000276:immunology*; D012764:Shigella sonnei / Q000276:immunology*; D013548:Sweden
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C063664:IpaA protein, Shigella flexneri; D008070:Lipopolysaccharides