A somatic cell hybrid map of human chromosome 13.
We have constructed a chromosome 13 somatic cell hybrid map using seven cell lines: PGMEA6, a hybrid containing the entire chromosome 13, and six hybrids containing various deletions of chromosome 13 (BARF7, PPF22, KBF11, KSF39, CF25, and CF27). We have mapped 80 markers that define 10 regions of chromosome 13 with respect to 10 breakpoints in the mapping panel; these regions range in size from 4 to 24 Mb, with an average size of 8 Mb. The 80 markers sublocalized on our mapping panel include 10 Alu-PCR clones, 6 of which were converted to sequence-tagged sites; 40 (CA)n repeat-containing clones, 27 of which are microsatellite PCR markers; 8 (AAAG)n repeat-containing PCR markers, 1 two-allele PCR marker, 4 genes or expressed sequences, and 17 anonymous DNA probes. This low-resolution physical map can be used as a backbone map for more refined physical mapping using radiation hybrids or yeast artificial chromosomes.
Washington
S S
SS
Department of Human Genetics, University of Pittsburgh, Pennsylvania 15261.
Bowcock
A M
AM
Gerken
S
S
Matsunami
N
N
Lesh
D
D
Osborne-Lawrence
S L
SL
Cowell
J
J
Ledbetter
D H
DH
White
R L
RL
Chakravarti
A
A
D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D006224:Cricetinae; D017931:DNA Primers; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D007621:Karyotyping; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
D017931:DNA Primers; D015342:DNA Probes; D005819:Genetic Markers