title: Oligonucleotide-poly-L-ornithine conjugates: binding to complementary DNA and RNA.
abstract: On the basis of the reported enhanced antisense activity of polylysine-oligonucleotide conjugates, a synthetic 12-mer oligodeoxyribonucleotide has been coupled at its 5' terminus to a series of positively charged (delta-ornithine)n cysteine peptides. Binding between the nucleic acid-peptide conjugate and its complementary DNA target sequence was detected by the impact of complexation on the melting temperature (Tm). It was found that the Tm for the nucleic acid-peptide gradually increased with increasing net charge on the conjugated peptide. Site-directed cleavage with RNase H demonstrates that the peptide-modified oligomer also hybridizes with its RNA target sequence. Increased affinity for target mRNA with net charge was shown by a cell-free translation arrest assay.
authors.affiliation: Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854.
authors.forename: T
authors.initials: T
authors.lastname: Zhu
authors.forename: Z
authors.initials: Z
authors.lastname: Wei
authors.forename: C H
authors.initials: CH
authors.lastname: Tung
authors.forename: W A
authors.initials: WA
authors.lastname: Dickerhof
authors.forename: K J
authors.initials: KJ
authors.lastname: Breslauer
authors.forename: D E
authors.initials: DE
authors.lastname: Georgopoulos
authors.forename: M J
authors.initials: MJ
authors.lastname: Leibowitz
authors.forename: S
authors.initials: S
authors.lastname: Stein
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011107:Polylysine / Q000378:metabolism; D014176:Protein Biosynthesis; D018075:RNA, Complementary / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D010455:Peptides; D018075:RNA, Complementary; C008973:polyornithine; D011107:Polylysine; D016914:Ribonuclease H