title: Localization of osteogenic protein-1 (bone morphogenetic protein-7) during human embryonic development: high affinity binding to basement membranes.
abstract: Osteogenic protein-1 (OP-1) is a member of the bone morphogenetic protein subfamily of the transforming growth factor-beta (TGF-beta) superfamily. Since members of the TGF-beta superfamily have a role in tissue development the distribution of OP-1 in developing human embryos (gestational age 5-14 weeks) was examined by immunohistochemical methods. Positive staining for OP-1 was observed in: sclerotome, hypertrophied chondrocytes, osteoblasts, periosteum, epithelial cells of the adrenal "provisional cortex" and the convoluted tubules of developing kidneys. In the developing lungs, pancreas and skin, OP-1 was localized in basement membranes underlying the epithelium. In vitro binding studies of 125I-OP-1 to various extracellular matrix components revealed high affinity of OP-1 for type IV collagen and less for heparin, collagen types I and VI. Present findings suggest that, in addition to bone formation, OP-1 could have other important regulatory roles in human embryogenesis with high binding affinity to a basement membrane component.
authors.affiliation: Department of Anatomy, School of Medicine, Zagreb, Croatia.
authors.forename: S
authors.initials: S
authors.lastname: Vukicevic
authors.forename: V
authors.initials: V
authors.lastname: Latin
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Batorsky
authors.forename: A H
authors.initials: AH
authors.lastname: Reddi
authors.forename: T K
authors.initials: TK
authors.lastname: Sampath
mesh_terms: D000311:Adrenal Glands / Q000033:anatomy & histology / Q000737:chemistry; D001485:Basement Membrane / Q000737:chemistry*; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000033:anatomy & histology / Q000737:chemistry; D002356:Cartilage / Q000033:anatomy & histology / Q000737:chemistry; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000033:anatomy & histology / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D016212:Transforming Growth Factor beta
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011506:Proteins; D016212:Transforming Growth Factor beta