Magnetic resonance imaging of neuronal grafts in the primate.
This preliminary study demonstrates the vital role that magnetic resonance imaging plays in the study of neuronal grafts in a nonhuman primate model of Huntington's disease. MRI scans were used to accurately locate and stereotactically lesion the anterior putamen of an adult rhesus monkey, thereby mimicking the anatomical and biochemical changes in Huntington's disease. Follow-up scans were utilized to confirm correct placement of the lesion and to stereotactically implant primate fetal neuronal tissue precisely into the lesion. Additionally, MRI appeared capable of imaging the fetal striatal grafts in rhesus monkeys. While the precise cellular composition of growing grafts was not identifiable on MRI scans, differentiation between normal, lesioned, and engrafted tissue was possible. Correlation of the magnetic resonance images of the grafted regions with actual graft tissue in perfused sections of the brain was shown. The use of magnetic resonance imaging, as indicated in these neuronal implantation studies, allows for a wide variety of future research possibilities where noninvasive and precise localization of the graft is mandated.
Simmons
N E
NE
Department of Neurological Surgery, University of Virginia Health Sciences Center, Charlottesville 22908.
Helm
G A
GA
Cail
W S
WS
Bennett
J P
JP
Jane
J A
JA
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D005260:Female; D016332:Fetal Tissue Transplantation; D007051:Ibotenic Acid / Q000494:pharmacology; D008253:Macaca mulatta; D008279:Magnetic Resonance Imaging; D008854:Microscopy, Electron; D009474:Neurons / Q000637:transplantation*; D013997:Time Factors
D007051:Ibotenic Acid