title: Fasciola hepatica: a secreted cathepsin L-like proteinase cleaves host immunoglobulin.
abstract: Adult Fasciola hepatica secrete a cysteine proteinase capable of cleaving host IgG close to the papain cleaving site. The proteinase was separated by size permeation chromatography. Gelatin-substrate polyacrylamide gel electrophoresis analysis revealed that the proteinase migrates as 6 proteolytic bands in the apparent molecular size range 60-90 kDa. Based on pH profiles of activity, inhibition studies using diethylpyrocarbonate and the diazomethylketone Z-phe-ala-CHN2, and characterising the substrate specificity of the enzymes using fluorogenic peptide substrates we have shown that the 60-90-kDa proteinases are cathepsin L-like proteinases.
authors.affiliation: School of Biological Sciences, Dublin City University, Republic of Ireland.
authors.forename: A M
authors.initials: AM
authors.lastname: Smith
authors.forename: A J
authors.initials: AJ
authors.lastname: Dowd
authors.forename: M
authors.initials: M
authors.lastname: Heffernan
authors.forename: C D
authors.initials: CD
authors.lastname: Robertson
authors.forename: J P
authors.initials: JP
authors.lastname: Dalton
mesh_terms: D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism*; D002417:Cattle; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D010450:Endopeptidases; D005210:Fasciola hepatica / Q000201:enzymology*; D007074:Immunoglobulin G / Q000378:metabolism*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D007074:Immunoglobulin G; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D056668:Cathepsin L