Effects of fatty acids on invasion through reconstituted basement membrane ('Matrigel') by a human breast cancer cell line.
An in vitro invasion assay system was used to examine the effects of linoleic acid, an omega-6 fatty acid, and two omega-3 fatty acids, eicosapentaenoic acid and docosahexaenoic acid, on the invasive capacity of MDA-MB-435 human breast cancer cells. Linoleic acid stimulated, and the omega-3 fatty acids inhibited, tumor cell invasion at concentrations of 0.25 and 0.5 microgram/ml. Indomethacin, 20 micrograms/ml, completely suppressed the stimulatory activity of linoleic acid, suggesting that these fatty acid effects are mediated via eicosanoid biosynthesis.
Connolly
J M
JM
Division of Nutrition and Endocrinology, American Health Foundation, Valhalla, NY 10595.
Rose
D P
DP
D000704:Analysis of Variance; D001485:Basement Membrane / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D003094:Collagen; D004338:Drug Combinations; D015777:Eicosanoids / Q000096:biosynthesis; D004967:Estrogens; D015525:Fatty Acids, Omega-3 / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007797:Laminin; D008041:Linoleic Acids / Q000378:metabolism / Q000633:toxicity*; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D009376:Neoplasms, Hormone-Dependent / Q000378:metabolism / Q000473:pathology; D011509:Proteoglycans; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004338:Drug Combinations; D015777:Eicosanoids; D004967:Estrogens; D015525:Fatty Acids, Omega-3; D007797:Laminin; D008041:Linoleic Acids; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D007213:Indomethacin