[A simultaneous determination of daidzin and puerarin and determination of daidzein in Oriental pharmaceutical decoctions containing puerariae radix by ion-pair high-performance liquid chromatography].
A simple and precise method was established for the simultaneous determination of daidzin and puerarin and the determination of daidzein in oriental pharmaceutical decoctions containing Puerariae Radix using high-performance liquid chromatography with tetra-n-heptylammonium bromide (THA) as an ion-pair reagent. Daidzin and puerarin were eluted within 45 min without interference with co-existing components using an ODS column and a mixture of 10 mM phosphate buffer (pH 6.5)-methanol (68:32) containing 5 mM THA as a mobile phase. Daidzein was eluted within 35 min without interference with co-existing components using an ODS column and a mixture of 10 mM phosphate buffer (pH 6.5)-acetonitrile (72:28 or 68:32) containing 5 mM THA as a mobile phase.
Yasuda
T
T
Tohoku College of Pharmacy, Miyagi, Japan.
Momma
N
N
Ohsawa
K
K
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C013908:daidzin; C033607:puerarin