[Study of the relationship of parthenogenetic forms of lizards of the species Lacerta and proposed parental bisexual species in comparison of DNA segments, considered by the polymerase chain reaction with a single primer].
Due to some suggestion [8] the parthenogenesis in vertebrates is the result of interspecies hybridization, and some species seem to be the main candidates participating in this process. DNA parts, which may be revealed using polymerase chain reaction in AP-RAPD modification, were compared in parthenogenetic lizards forms and in supposed parental species for them. As it was shown by other authors and confirmed by us, arbitrary primed randomly amplified DNA products are species specific and may be used as a molecular taxonomic criterion. It was shown that each parthenogenetic species studied has the patterns containing only the bands of one or another supposed parents and no more. This is in favour of the hypothesis mentioned above. Investigation of 103 10-nucleotide primers in PCR was done.
Grechko
V V
VV
Kataev
M V
MV
Mel'nikova
M N
MN
Darevskiĭ
I S
IS
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D008116:Lizards / Q000235:genetics* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D010312:Parthenogenesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D004247:DNA
Issledovanie rodstvennykh sviazeĭ partenogeneticheskikh form iashcherits roda Lacerta i predpolagaemykh roditel'skikh dvupolykh vidov pri sravnenii chasti DNK, schityvaemoĭ v tsepnoĭ polimeraznoĭ reaktsii s edinichnym praĭmerom.