Genes encoding antigenic surface glycoproteins in Pneumocystis from humans.
Pneumocystis is a eukaryotic microbe that causes pneumocystosis, an AIDS-associated pneumonia. Pneumocystosis also occurs in many other mammalian species, and animal-derived organisms have been extensively utilized in Pneumocystis research. Pneumocystis from diverse hosts contain a large glycoprotein (gpA/MSG) on the surface. Antibodies elicited against gpA/MSG of Pneumocystis from humans sometimes cross-react with epitopes on proteins of similar size from Pneumocystis from other host species. Here we report the isolation and partial sequence of two presumptive gpA/MSG genes from human-derived Pneumocystis. The cloned human-derived Pneumocystis gpA/MSG genes and predicted peptides were different from those previously isolated from Pneumocystis from rats and ferrets. The genome of human-derived Pneumocystis contained multiple copies of sequences related to the two cloned gpA/MSG genes.
Stringer
S L
SL
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, College of Medicine, Ohio 45267.
Garbe
T
T
Sunkin
S M
SM
Stringer
J R
JR
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000595:Amino Acid Sequence; D000818:Animals; D000946:Antigens, Fungal / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis; D005800:Genes, Fungal; D006801:Humans; D008168:Lung / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016720:Pneumocystis Infections / Q000209:etiology / Q000382:microbiology; D051381:Rats; D011914:Rats, Inbred BN; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D000946:Antigens, Fungal; D004271:DNA, Fungal; D008562:Membrane Glycoproteins