title: Growth and development of bone mass in untreated alloxan diabetic rats. Effects of collagen glycosylation and parathyroid activity on bone turnover.
abstract: Body and skeletal growth and development were studied in alloxan-treated and age-matched control rats, between 3 and 23 weeks of age. For both groups the growth of the skeletal and body weights were in phase, with a maximum at 7 weeks of age. The growth data was assessed according to Parks' theory of feeding and growth. Alloxan-treated rats showed an important reduction in body and bone mass, with a greater impact on soft tissues. As expected, the asymptotic body and skeletal weights were reduced respect to controls. The time needed to attain 63% of mature food intake (Brody's 'time constant') was also reduced, indicating that maturation occurred at an earlier age than controls. The diabetic state is characterized by a reduced food conversion efficiency. Despite hyperfagia, alloxan-treated rats showed circa one-half the body and skeletal weights of age-matched controls. The following adverse effects of alloxan diabetes on bone tissue were observed: (a) a decrease in trabecular bone volume (femoral metaphyses) and cortical width (femoral diaphyses), (b) increased bone collagen glycosylation as a function of extracellular glucose concentration, (c) increased resistance of bone collagen to collagenase hydrolysis, (d) decreased rate of bone resorption except under strongly stimulated parathyroid function, (d) significantly lower ashes/bone matrix ratio in diabetic rats with more than 10 weeks of diabetes, and (e) no histological evidence of osteomalacia.
authors.affiliation: Laboratorio de Biologia Osea, Facultad de Ciencias Medicas, Rosario, Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Locatto
authors.forename: H
authors.initials: H
authors.lastname: Abranzon
authors.forename: D
authors.initials: D
authors.lastname: Caferra
authors.forename: M C
authors.initials: MC
authors.lastname: Fernandez
authors.forename: R
authors.initials: R
authors.lastname: Alloatti
authors.forename: R C
authors.initials: RC
authors.lastname: Puche
mesh_terms: D000496:Alloxan; D000818:Animals; D001835:Body Weight; D015519:Bone Density; D001846:Bone Development / Q000502:physiology*; D001862:Bone Resorption; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology; D003094:Collagen / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D006031:Glycosylation; D008297:Male; D010280:Parathyroid Glands / Q000502:physiology*; D051381:Rats
chemical_list: D000496:Alloxan; D003094:Collagen