Comparative studies of asparagine-linked sugar chains of immunoglobulin G from eleven mammalian species.
1. Asparagine-linked sugar chains released by hydrazinolysis from IgGs of porcine, equine, bovine, goat, ovine, canine, rabbit, guinea-pig and rat were comparatively analyzed by microsequencing and lectin affinity chromatography. 2. Sugar chains of all IgGs basically consisted of biantennary complex-type oligosaccharides containing 0-2 sialic acid residue(s). More than 70% of the oligosaccharides were neutral, except for guinea-pig IgG, and fucosylated trimannosyl core structures were dominant except for rabbit IgG. Bisecting N-acetylglucosamine residue was absent in porcine and equine IgGs. 3. A large quantity of galactose-less oligosaccharides were present in IgGs of porcine, equine, canine and rat.
Hamako
J
J
Division of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Fujita Health University, Aichi, Japan.
Matsui
T
T
Ozeki
Y
Y
Mizuochi
T
T
Titani
K
K
D000818:Animals; D001216:Asparagine / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D005690:Galactose / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D037102:Lectins; D008322:Mammals / Q000276:immunology*; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D012794:Sialic Acids / Q000032:analysis
D007074:Immunoglobulin G; D037102:Lectins; D009844:Oligosaccharides; D012794:Sialic Acids; D001216:Asparagine; D019158:N-Acetylneuraminic Acid; D005690:Galactose