On the tertiary structure of the Citrus ichangensis satellite DNA.
A hexamer of the repeating unit of Citrus ichangensis satellite DNA was cloned. Polyacrylamide gel electrophoresis demonstrated that the shape of cloned hexamer is other than linear. As the computing of tertiary coordinates made by Eckdahl and Anderson's BEN program proved, the hexamer is a solenoid consisting of two turns termed coiled double helix (CDH)-form. An electron microscopic analysis revealed small diameter circles in the hexamer under investigation. Unlike the hexamer control molecules are s-shaped. It is concluded that the CDH-form is a characteristic of the satellite DNA of Citrus ichangensis.
Beridze
T
T
Institute of Plant Biochemistry, Georgian Academy of Sciences, Tbilisi.
Tsirekidze
N
N
Turishcheva
M S
MS
D002957:Citrus / Q000235:genetics; D003001:Cloning, Molecular; D003198:Computer Simulation; D004276:DNA, Satellite / Q000737:chemistry* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012984:Software
D004276:DNA, Satellite; D046911:Macromolecular Substances