Temporal sequence of changes in tear film composition during sleep.
Overnight eye closure induces a shift in the nature and composition of the tear film, from a dynamic reflex tear-rich to a stagnant secretory IgA-rich layer. This is accompanied by the induction of a state of sub-clinical inflammation, as evidenced by increases in albumin levels, plasminogen activation, conversion of complement C3 to C3c, and the recruitment of polymorphonuclear (PMN) cells into the tear film. To determine the time course and functional relationship between these potentially interdependent processes, tear samples were collected from ten non-contact lens wearers after 1, 2, 3 and 5 hours of sleep. A subgroup of 6 subjects also self-collected tear samples after 8 hours of sleep. Tear samples were analysed for albumin by quantitative immunofixation assay, secretory IgA (sIgA) by radial immunodiffusion assay, plasmin-like activity using a chromogenic substrate, and complement C3 to C3c conversion by immunoblot assay. Epithelial and PMN cells in the precorneal tear film were recovered from corneal washings from the same subjects after 1, 3, 5 and 8 hours of sleep, and quantified. Results revealed that, unlike epithelial cells which exhibited a slow progressive accumulation as a function of the period of sleep, PMN cell concentration exhibited a lag phase, with recruitment occurring after between 3 and 5 hours of eye closure. This was preceded by plasminogen activation, increases in albumin and sIgA levels, and complement C3 to C3c conversion, all of which occurred within 1 to 3 hours after eye closure. Plasmin-like activity appeared to plateau after 3 hours and then decreased.(ABSTRACT TRUNCATED AT 250 WORDS)
Tan
K O
KO
Cooperative Research Centre for Eye Research and Technology, School of Optometry, University of New South Wales, Sydney, Australia.
Sack
R A
RA
Holden
B A
BA
Swarbrick
H A
HA
D000328:Adult; D000418:Albumins / Q000378:metabolism; D003176:Complement C3 / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D012890:Sleep / Q000502:physiology*; D013666:Tears / Q000378:metabolism*
D000418:Albumins; D003176:Complement C3; D005136:Eye Proteins; D007071:Immunoglobulin A, Secretory; C042434:tear proteins; D005341:Fibrinolysin