title: Gamma delta T-cell lines isolated from intestinal epithelium respond to a B-cell lymphoma.
abstract: gamma delta T-cell hybrid clones were obtained from intestinal intraepithelial lymphocytes (i-IEL) by fusion with the BW5147 thymoma line. Four clones which expressed different V gamma/V delta genes were selected for further study. All of the gamma delta clones secreted interleukin-2 (IL-2) in the presence of the BALB/c-derived B-lymphoma line, A20. No alpha beta T-cell hybrid clones derived from spleen or i-IEL responded to A20. We obtained several pieces of evidence which strongly suggest that these responses are mediated by the gamma delta T-cell receptor (TcR). Class II major histocompatibility complex (MHC), FcR and surface Ig expressed on A20 are not involved in the response. Native i-IEL derived from BALB/c selectively survive in culture in the presence of A20 cells. The ligand may be a superantigen-like molecule because all our gamma delta T-cell clones responded to A20 in spite of their different combinations of V gamma/V delta gene segments.
authors.affiliation: Howard Hughes Medical Institute, Yale University School of Medicine, Connecticut, New Haven 06510.
authors.forename: Y
authors.initials: Y
authors.lastname: Sano
authors.forename: E
authors.initials: E
authors.lastname: Dudley
authors.forename: S
authors.initials: S
authors.lastname: Carding
authors.forename: R H
authors.initials: RH
authors.lastname: Lin
authors.forename: A C
authors.initials: AC
authors.lastname: Hayday
authors.forename: C A
authors.initials: CA
authors.lastname: Janeway
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D007413:Intestinal Mucosa / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D016393:Lymphoma, B-Cell / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis* / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D014373:Tuberculin; D004247:DNA