Establishment of lung cancer cell line producing parathyroid hormone-related protein.
Parathyroid hormone-related protein (PTHrP) producing cell line (KCC-C1) was established from malignant pleural effusion of a patient with squamous cell lung carcinoma. Hypercalcemia and granulocytosis were noted in the patient. The serum level of PTHrP, measured by N-terminal specific radioimmunoassay, was 110 pg/ml (normal < 20 pg/ml). The established KCC-C1 tumor cells proved to have PTHrP RNA transcripts and produce a large amount of PTHrP. Besides the production of PTHrP, the culture medium contained a significant level of interleukin 1 (IL-1). However, tumor necrosis factor or colony stimulating factor was not defected. Transplantation of KCC-C1 tumor cells into nude mice resulted in tumor formation with hypercalcemia. As IL-1 is also known to have bone-resorbing activity, KCC-C1 which may prove valuable in the study of the interaction between PTHrP and IL-1 for induction of hypercalcemia.
Ichinose
Y
Y
Department of Chest Surgery, National Kyushu Cancer Center, Fukuoka, Japan.
Iguchi
H
H
Ohta
M
M
Katakami
H
H
D000818:Animals; D015152:Blotting, Northern; D001862:Bone Resorption / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D002869:Chromosome Aberrations; D017809:Fatal Outcome; D006801:Humans; D006934:Hypercalcemia / Q000209:etiology* / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D007621:Karyotyping; D008175:Lung Neoplasms / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D009368:Neoplasm Transplantation; D044162:Parathyroid Hormone-Related Protein; D010996:Pleural Effusion / Q000473:pathology; D011506:Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D007375:Interleukin-1; D009363:Neoplasm Proteins; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins