Assignment of the human PAX4 gene to chromosome band 7q32 by fluorescence in situ hybridization.
Of the nine known members of a human paired box-containing gene family (Pax), only PAX4 has not been precisely localized. We screened a cosmid library of human genomic DNA using polymerase chain reaction products for PAX4 as a probe and isolated three positive cosmid clones. Sequence analysis revealed that at least two of them had exon-like sequences and showed extensive homology to Pax-4 in the mouse. These two cosmid clones were mapped to human chromosome band 7q32 by fluorescence in situ hybridization.
Tamura
T
T
Department of Human Genetics, Nagasaki University School of Medicine, Japan.
Izumikawa
Y
Y
Kishino
T
T
Soejima
H
H
Jinno
Y
Y
Niikawa
N
N
D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004268:DNA-Binding Proteins; D004247:DNA