title: Crystallization and a preliminary X-ray diffraction study of macrophage migration inhibitory factor from human lymphocytes.
abstract: Two crystal forms of the macrophage migration inhibitory factor (MIF) from human lymphocytes have been obtained by the hanging drop method of vapor diffusion from ammonium sulfate solution. A trigonal crystal form belongs to the space group P3(1)21 (P3(2)21), with unit cell dimensions of a = b = 96.4 A and c = 105.5 A. Assuming that the asymmetric unit contains two or three dimers, the Vm value is calculated as 2.9 or 1.9, respectively. A second crystal form is orthorhombic, space group P2(1)2(1)2(1) with unit cell dimensions a = 68.4 A, b = 68.8 A and c = 86.8 A. The Vm value is 2.1 for two dimers in the asymmetric unit. Both crystals diffract to at least 1.9 A and are suitable for X-ray crystallographic structure determination at a high resolution.
authors.affiliation: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: E
authors.initials: E
authors.lastname: Murata
authors.forename: I
authors.initials: I
authors.lastname: Tanaka
authors.forename: J
authors.initials: J
authors.lastname: Nishihira
authors.forename: M
authors.initials: M
authors.lastname: Sakai
mesh_terms: D001483:Base Sequence; D018360:Crystallography, X-Ray; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D008263:Macrophage Migration-Inhibitory Factors / Q000737:chemistry*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins
chemical_list: D017931:DNA Primers; D008263:Macrophage Migration-Inhibitory Factors; D011994:Recombinant Proteins