Subchromosomal localization of a gene (XRCC5) involved in double strand break repair to the region 2q34-36.
We have previously shown that human chromosome 2 can complement both the radiation sensitivity and the defect in double strand break rejoining characteristic of ionizing radiation (IR) group 5 mutants. A number of human-hamster hybrids containing segments of human chromosome 2 were obtained by microcell transfer into two group 5 mutants. In most, but not all, of these hybrids, the repair defect was complemented by the human chromosomal DNA. Two complementing microcell hybrids were irradiated and fused to XR-V15B, an IR group 5 mutant, to generate further hybrids bearing smaller regions of chromosome 2. All hybrids were examined for complementation of the repair defect. The region of chromosome 2 present was determined using PCR with primers specific for various human genes located on chromosome 2. A complementing hybrid bearing only a small region of chromosome 2 was finally generated. From this analysis we deduced that the XRCC5 gene was tightly linked to the marker, TNP1, which is located in the region 2q35.
Hafezparast
M
M
MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, U.K.
Kaur
G P
GP
Zdzienicka
M
M
Athwal
R S
RS
Lehmann
A R
AR
Jeggo
P A
PA
D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D018014:Gene Transfer Techniques; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011836:Radiation Tolerance / Q000235:genetics*; D012641:Selection, Genetic
D005819:Genetic Markers