Evaluation of serological pseudorabies tests for the detection of antibodies during early infection.
Six enzyme-linked immunosorbent assays, a latex agglutination test, and the standard microtitration serum virus neutralization test were compared for their ability to detect antibodies against pseudorabies virus (PRV) during the early stages of infection. Thirty-five pigs were infected intranasally with 10(5)-10(7) TCID50 of either the Iowa 4892 pneumotropic or the Becker strain of PRV. Blood samples were drawn from experimentally inoculated animals on days 4-10, 14, and 21 postchallenge. Test sensitivity estimates and comparisons among tests were made for each sampling day over the 21-day monitoring period. Results of this study demonstrated differences among tests in 1) the time from inoculation to initial antibody detection, 2) the time to detect > or = 95% of the infected pigs, and 3) the time from initial antibody detection to determination of > or = 95% as positive. By day 10 postchallenge, no statistically significant difference in diagnostic sensitivity was observed among the 8 tests compared in the study.
Oren
S L
SL
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011.
Swenson
S L
SL
Kinker
D R
DR
Hill
H T
HT
Hu
H L
HL
Zimmerman
J
J
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D007841:Latex Fixation Tests / Q000379:methods / Q000662:veterinary; D009500:Neutralization Tests / Q000379:methods / Q000662:veterinary; D011557:Pseudorabies / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D013552:Swine; D013553:Swine Diseases; D013997:Time Factors
D000914:Antibodies, Viral