Different rates of interleukin 2 receptor expression by ovine gamma/delta and alpha/beta T cells.
We have used the monoclonal antibodies IL-A111, CACT116A, to investigate the expression of interleukin 2 receptors (IL-2R) by ovine peripheral blood T cells. Monoclonal antibodies (MAbs) IL-A111 and CACT116A reacted with concanavalin A (Con A) activated ovine lymphocytes and both mAbs did so with similar FACS (fluorescence activated cell sorting) profiles and fluorescence intensity. Following activation by Con A all three major ovine T cell subsets, namely CD4+, CD8+ and gamma/delta T cells, express the IL-2R. All gamma/delta T cells express IL-2R within 12 h following activation by Con A in vitro whereas alpha/beta T cells do not express the IL-2R until 24 h after the start of culture with the mitogen. In freshly isolated peripheral blood a small percentage of lymphocytes, which are almost all CD4+, express the IL-2R.
Bujdoso
R
R
Department of Veterinary Pathology, University of Edinburgh, UK.
Lund
B T
BT
Evans
C W
CW
McConnell
I
I
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D003208:Concanavalin A; D005434:Flow Cytometry / Q000662:veterinary; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2; D003208:Concanavalin A