A novel transcript from a pseudogene for human glucocerebrosidase in non-Gaucher disease cells.
Human glucocerebrosidase (GC)-encoding cDNA clones were isolated from a promyelocytic HL-60 cDNA library and analyzed. A novel cDNA clone was found to originate from a gene referred to as a GC pseudogene. Using the polymerase chain reaction (PCR) with primers specific for the GC pseudogene, we found that all the human cell lines examined, HL-60, K-562, WI-38, HepG2 and HeLa, expressed a pseudogene transcript. In vitro translation of RNA synthesized by transcription of the pseudogene cDNA produced a polypeptide of approximately 30 kDa.
Imai
K
K
Department of Biochemistry, Shimane Medical University, Izumo, Japan.
Nakamura
M
M
Yamada
M
M
Asano
A
A
Yokoyama
S
S
Tsuji
S
S
Ginns
E I
EI
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005776:Gaucher Disease / Q000235:genetics; D005962:Glucosylceramidase / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011544:Pseudogenes
D018076:DNA, Complementary; D005962:Glucosylceramidase