title: Direct peptide mapping of sickle-cell hemoglobin using electrospray mass spectrometry.
abstract: Direct peptide mapping of sickle-cell hemoglobin (Hb S) using electrospray mass spectrometry is demonstrated to be a rapid, sensitive and useful technique for providing information concerning the location of variation. By fully comparing electrospray mass spectra of digests of sickle-cell and normal hemoglobins, using L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin, the point mutation of B1 fragment of beta-chain in Hb S can be confirmed, taking into consideration that all the other fragments are completely identical in the two proteins.
authors.affiliation: Central Research Laboratory, Hitachi Ltd., Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakairi
mesh_terms: D000595:Amino Acid Sequence; D006451:Hemoglobin, Sickle / Q000235:genetics*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*
chemical_list: D006451:Hemoglobin, Sickle