title: cDNA-derived identification of novel thionin precursors in Viscum album that contain highly divergent thionin domains but conserved signal and acidic polypeptide domains.
abstract: The existence of new thionin variants in Viscum album has been deduced from cDNA sequences. Unlike the viscotoxins and related thionins previously found in different members of the Viscaceae, these novel thionins contain eight rather than six cysteine residues. In this respect they resemble thionins described previously from various cereals and from Pyrularia pubera, which also contain eight cysteine residues at identical positions. All of the new thionins of V. album are encoded as higher-molecular-weight precursors consisting of a signal peptide, a thionin domain and an acidic polypeptide domain. While the deduced amino acid sequences of the thionin domains of different precursor molecules are highly divergent, the two other domains are conserved among all of the variants and are distinct from the corresponding domains of thionin precursors of other plant species.
authors.affiliation: Swiss Institute of Technology (ETH), Institut für Pflanzenwissenschaften, Zürich.
authors.forename: G
authors.initials: G
authors.lastname: Schrader-Fischer
authors.forename: K
authors.initials: K
authors.lastname: Apel
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary; D015870:Gene Expression; D017343:Genes, Plant; D007150:Immunohistochemistry; D014784:Mistletoe / Q000235:genetics*; D008969:Molecular Sequence Data; D028321:Plant Preparations; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010946:Plants, Medicinal; D010947:Plants, Toxic / Q000737:chemistry; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D054790:Ribosome Inactivating Proteins, Type 2; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014118:Toxins, Biological / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D028321:Plant Preparations; D010940:Plant Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum; C488038:viscotoxin