Perturbation of glutathione status and generation of oxidative stress in mouse skin following application of contact allergenic sesquiterpene lactones and isothiocyanates.
1. The sensitizing or non-sensitizing status of selected sesquiterpene lactones and isothiocyanates was confirmed in mouse by open epicutaneous application. 2. Glutathione status of mouse skin was determined 12 h after lactone/isothiocyanate application; glutathione S-transferase activity also was determined 12 h after lactone application. 3. NAD(P)H utilization by rat liver microsomal preparations exposed to the sesquiterpene lactones and isothiocyanates was measured. 4. A correlation was observed between sensitizing status and the ability to perturb glutathione status, to induce glutathione S-transferase activity, and to stimulate NAD(P)H utilization. 5. It was concluded that sensitizing sesquiterpene lactones and isothiocyanates could induce oxidative stress in mouse skin, possibly as a result of their reductive metabolism.
Schmidt
R J
RJ
Welsh School of Pharmacy, UWCC, Cardiff, UK.
Chung
L Y
LY
D000279:Administration, Cutaneous; D000485:Allergens; D000818:Animals; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D007114:Immunization; D017879:Isothiocyanates / Q000008:administration & dosage*; D007783:Lactones / Q000008:administration & dosage*; D051379:Mice; D015394:Molecular Structure; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000737:chemistry*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism
D000485:Allergens; D017879:Isothiocyanates; D007783:Lactones; D012717:Sesquiterpenes; D009249:NADP; D005982:Glutathione Transferase; D005978:Glutathione