title: [Restriction endonuclease analysis of leptospiral DNA from different serogroup and serovar].
abstract: The DNAs of different serogroups and serovars of leptospires were analyzed with seven kinds of restriction endonuclease (Bam HI, Bgl II, Cfo I, Eco RI, Hind III, Kpn I and Pst I). The results showed that there are significant differences among the leptospiral DNAs of different serovars, especially in the high molecular-weight region, but the patterns of different strains belonging to the same serovar are similar. In addition, Cfo I and Eco RI are more effective for differentiating leptospires.
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.forename: B
authors.initials: B
authors.lastname: Dai
mesh_terms: D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI; D007919:Leptospira / Q000145:classification* / Q000302:isolation & purification; D007921:Leptospira interrogans / Q000145:classification / Q000302:isolation & purification; D015183:Restriction Mapping; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI