title: In vitro conversion of recombinant human proapolipoprotein A-I to apolipoprotein A-I.
abstract: We recently investigated the in vivo conversion of recombinant human proapolipoprotein A-I (rh-Met-proapo A-I) from E. coli to apolipoprotein (apo) A-I in rabbits. In vitro incubation of rh-Met-proapo A-I with rabbit serum produced mature apo A-I3 isoproteins, as determined by the immunoblotting method. However, at the time we were unable to chemically confirm a newly produced protein band which appeared at the position of human apo A-I3. Since then, we have confirmed the amino acid sequence of the protein using a membrane protein sequence technique, and have concluded that it corresponds to human apo A-I3.
authors.affiliation: Department of Internal Medicine, Fukuoka University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saku
authors.forename: H
authors.initials: H
authors.lastname: Bai
authors.forename: K
authors.initials: K
authors.lastname: Hirata
authors.forename: R
authors.initials: R
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Ohkubo
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Arakawa
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry*; D001054:Apolipoproteins A / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry*; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D000596:Amino Acids; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D011498:Protein Precursors; D011994:Recombinant Proteins