Cloning of the cDNA and the genomic DNA of the mouse angiotensin II type 2 receptor.
Of the two major isoforms of the angiotensin II receptors, type 1 (AT1) and type 2 (AT2), little is known about the structure and features of AT2. We cloned a mouse AT2 cDNA from a mouse fetus cDNA library and an AT2 genomic DNA from a 129SV mouse genomic DNA library. The amino acid sequence of the mouse AT2 (363 residues) deduced from a mouse cDNA clone showed seven membrane-spanning domains. Amino acid identity of the mouse AT2 with mouse AT1 is 37%, and 98% with rat AT2. The genomic DNA (4.4 kb) contained three exons and two introns and the entire coding region was contained in the third exon.
Ichiki
T
T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Herold
C L
CL
Kambayashi
Y
Y
Bardhan
S
S
Inagami
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis*; D005091:Exons; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D051381:Rats; D011945:Receptors, Angiotensin / Q000235:genetics*
D018076:DNA, Complementary; D011945:Receptors, Angiotensin