Quantitative assessment of oestrogen and progesterone receptors on fine-needle aspirates and cryostat sections of breast carcinomas: how does this compare with biochemical assays?
In this study monoclonal antibodies to oestrogen receptors in the ER-ICA kit (Abbott) and to progesterone receptors (Biogenex) were used to determine the presence of those receptors in fine needle aspirates and cryostat sections. The degree of positivity is semi-quantitated and the results compared to biochemical assays (Dextran-Coated Charcoal Method) performed on the same specimens. Out of 14 cases that were positive on the biochemical assay, 11 (77%) were positive on ER-ICA performed on the FNA material, and there was a 100% agreement on the cryostat sections (11/11). PR-ICA on the cytospin smears and cryostat sections agreed in 63% and 90% respectively.
Muchiri
L W
LW
Department of Pathology, College of Health Sciences, University of Nairobi, Kenya.
D000911:Antibodies, Monoclonal; D001707:Biopsy, Needle / Q000379:methods*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000453:epidemiology / Q000473:pathology*; D015925:Cryopreservation; D005069:Evaluation Studies as Topic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D011960:Receptors, Estrogen / Q000032:analysis* / Q000276:immunology; D011980:Receptors, Progesterone / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone