Dual role of calsequestrin as substrate and inhibitor of casein kinase-1 and casein kinase-2.
Calsequestrin from different muscle tissues and species has been phosphorylated by casein kinase-1 and casein kinase-2, in the conditions previously reported by Cala and Jones (J. Biol. Chem. 266, 391-398, 1991). Results indicates that rabbit cardiac and skeletal calsequestrin and frog skeletal calsequestrin are phosphorylated by both casein kinase-1 and casein kinase-2, at variance with chicken skeletal calsequestrin which is a poor substrate for both enzymes. We also observed that chicken calsequestrin is able to inhibit phosphorylation of cardiac calsequestrin, as well as other specific substrates, when added together to the assay medium.
Salvatori
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S
CNR Centro di Studio per la Biologia e la Fisiopatologia Muscolare, Biomediche Sperimentali, Università di Padova, Italy.
Furlan
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Meggio
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F
D000595:Amino Acid Sequence; D000818:Animals; D002155:Calsequestrin / Q000378:metabolism* / Q000494:pharmacology*; D047388:Casein Kinases; D002645:Chickens; D004285:Dogs; D007700:Kinetics; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology; D009928:Organ Specificity; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D011898:Ranidae; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013379:Substrate Specificity
D002155:Calsequestrin; D009842:Oligopeptides; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; D047388:Casein Kinases