title: Toluene diisocyanate-stimulated release of arachidonic acid metabolites in the organ bath from guinea-pig airways.
abstract: This study was designed to evaluate whether metabolites of arachidonic acid play a role in the contractile response to toluene diisocyanate in isolated guinea pig airways. In control experiments we collected the supernatant from an organ bath over a time period of 2 h, after the addition of toluene diisocyanate (100 and 300 microM), and after the addition of toluene diisocyanate (300 microM) in the presence of indomethacin (5 microM). We measured prostaglandin E2, 6-keto-prostaglandin F1 alpha, prostaglandin F2 alpha, thromboxane B2, leukotriene B4, leukotriene C4/D4/E4/F4 by radioimmunoassays. Levels of prostaglandin F2 alpha and 6-keto-prostaglandin F1 alpha increased significantly after addition of toluene diisocyanate in the absence of indomethacin. These results suggest that prostaglandins are involved in toluene diisocyanate-induced contractions in guinea-pig airways.
authors.affiliation: Institute of Occupational Medicine, University of Padua, Italy.
authors.forename: C E
authors.initials: CE
authors.lastname: Mapp
authors.forename: A
authors.initials: A
authors.lastname: Boniotti
authors.forename: M
authors.initials: M
authors.lastname: Masiero
authors.forename: M
authors.initials: M
authors.lastname: Plebani
authors.forename: A
authors.initials: A
authors.lastname: Burlina
authors.forename: A
authors.initials: A
authors.lastname: Papi
authors.forename: P
authors.initials: P
authors.lastname: Maestrelli
authors.forename: M
authors.initials: M
authors.lastname: Saetta
authors.forename: A
authors.initials: A
authors.lastname: Ciaccia
authors.forename: L
authors.initials: L
authors.lastname: Fabbri
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D011453:Prostaglandins / Q000378:metabolism; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology*
chemical_list: D001095:Arachidonic Acids; D011453:Prostaglandins; D014051:Toluene 2,4-Diisocyanate; D007213:Indomethacin