title: 11 beta-Hydroxylase gene expression in the rat adrenal cortex.
abstract: It is now known that in the rat there are two distinct species of cytochrome P450(11 beta/18), namely aldosterone synthase and 11 beta-hydroxylase. Whereas aldosterone synthase is located exclusively in the zona glomerulosa, the zonal distribution and site of production of 11 beta-hydroxylase is not entirely clear. In the present study we examined the zonal expression of 11 beta-hydroxylase mRNA in adrenals from control rats and animals subjected to ACTH treatment and dietary sodium restriction using a non-isotopic in-situ hybridization technique. The results were compared with those obtained using an inner zone specific antigenic (IZAg) marker to give unequivocal identification of the adrenocortical cell types. 11 beta-Hydroxylase mRNA was clearly shown to be expressed in the inner zones of the control rat adrenal cortex, and none was detected in the zona glomerulosa and medulla. The message was more abundant in the outer zona fasciculata. A similar pattern of distribution of 11 beta-hydroxylase mRNA was observed in adrenals from rats subjected to dietary sodium restriction, although the width of the negatively staining layer of zona glomerulosa was significantly increased. In rats treated with 100 micrograms ACTH for 1 day, the number of cells expressing 11 beta-hydroxylase mRNA was increased, especially in the zona reticularis. With continued ACTH treatment, 11 beta-hydroxylase mRNA was found in the region of the zona glomerulosa, and after 3 and 5 days of ACTH treatment cells expressing 11 beta-hydroxylase mRNA extended to the connective tissue capsule. At this time there was a significant reduction in the total expression of the message compared with the controls.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biochemistry, Queen Mary and Westfield College, London, U.K.
authors.forename: M M
authors.initials: MM
authors.lastname: Ho
authors.forename: G P
authors.initials: GP
authors.lastname: Vinson
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects / Q000201:enzymology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D005260:Female; D015870:Gene Expression / Q000502:physiology; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D012982:Sodium, Dietary / Q000008:administration & dosage; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics*; D015384:Zona Glomerulosa / Q000201:enzymology; D015385:Zona Reticularis / Q000201:enzymology
chemical_list: D012333:RNA, Messenger; D012982:Sodium, Dietary; D000324:Adrenocorticotropic Hormone; D013252:Steroid 11-beta-Hydroxylase