Effects of pyrethroid insecticides on gap junctional intercellular communications in Balb/c3T3 cells by dye-transfer assay.
The effects of fenvalerate, esfenvalerate, permethrin, cypermethrin, deltamethrin, p-chlorophenylisovaleric acid (CPIA, major metabolite of fenvalerate) and DDT, a liver tumor promoter, on gap junctional intercellular communication (GJIC) were examined in Balb/c3T3 cells by dye-transfer assay. Separate groups of Balb/c3T3 cells were exposed to the chemicals for 1 day. On the following day, GJIC was measured by counting the number of dye-transferring cells per injection of Lucifer Yellow under a fluorescent microscope. Fenvalerate, esfenvalerate, permethrin, cypermethrin, deltamethrin and DDT inhibited GJIC at noncytotoxic concentrations, while CPIA did not inhibit GJIC even at a cytotoxic concentration. It is concluded that the examined pyrethyroid insecticides, but not a metabolite, have inhibitory effects on GJIC in Balb/c3T3 cells.
Tateno
C
C
Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd, Osaka, Japan.
Ito
S
S
Tanaka
M
M
Yoshitake
A
A
D016475:3T3 Cells / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002450:Cell Communication / Q000187:drug effects* / Q000502:physiology; D003634:DDT / Q000633:toxicity; D005456:Fluorescent Dyes; D017629:Gap Junctions / Q000187:drug effects* / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D007546:Isoquinolines; D051379:Mice; D008807:Mice, Inbred BALB C; D009570:Nitriles; D026023:Permethrin; D011722:Pyrethrins / Q000378:metabolism / Q000633:toxicity*
D005456:Fluorescent Dyes; D007306:Insecticides; D007546:Isoquinolines; D009570:Nitriles; D011722:Pyrethrins; C017160:cypermethrin; C017180:decamethrin; D026023:Permethrin; C017475:lucifer yellow; D003634:DDT; C017690:fenvalerate