Characterization of insoluble macromolecular Sn(II) complex and its application to the 99mTc labeling of human serum albumin-bearing mercapto groups.
A chelating ion exchange resin containing aminophosphonic acid groups was used as a polymer matrix for the preparation of an insoluble macromolecular Sn(II)(R-Sn) complex. Sn(II), which strongly bound to the surface of the polymer matrix by chelation, retained the ability to reduce 99mTc in the R-Sn complex. Human serum albumin-bearing-mercapto groups (HMA) was labeled with 99mTc at pH 2-3 using the R-Sn complex or SnCl2 as reducing agent. The 99mTc-HMA labeled with the R-Sn complex resulted in a higher sustained level of radioactivity in the blood of mice than the 99mTc-HMA labeled with SnCl2. These results suggest that the use of the R-Sn complex minimized Sn(II) contamination of the 99mTc labeling solution and can be used effectively as a reducing agent for 99mTc labeling of proteins containing sulfhydryl groups.
Nakayama
M
M
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Terahara
T
T
Ikeda
R
R
Wada
M
M
Koiso
T
T
Kimura
Y
Y
Harada
K
K
Sugii
A
A
Tomiguchi
S
S
Kojima
A
A
D000818:Animals; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D013438:Sulfhydryl Compounds; D013668:Technetium Tc 99m Aggregated Albumin; D014001:Tin
D046911:Macromolecular Substances; D013438:Sulfhydryl Compounds; D013668:Technetium Tc 99m Aggregated Albumin; D014001:Tin