Isolation of cDNAs encoding thymidylate synthase from soybean seedlings and expression of the protein in E. coli.
Wang
M
M
Department of Biochemistry and Molecular Biology, Medical College of Ohio, Toledo 43699-0008.
Ratnam
S
S
Freisheim
J H
JH
D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology; D005492:Folic Acid / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D007780:Lacticaseibacillus casei / Q000201:enzymology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics; D013938:Thymidine Monophosphate / Q000378:metabolism; D013940:Thymidylate Synthase / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification
D018076:DNA, Complementary; D011994:Recombinant Proteins; D013938:Thymidine Monophosphate; D005492:Folic Acid; D013940:Thymidylate Synthase