Differential immunocytochemical localization of GABAA receptor gamma 1 and gamma 2 subunits in the rat brain.
Subunit-specific polyclonal antisera against the GABAA receptor gamma 1 and gamma 2 subunits were raised in rabbits and used for immunoblotting and immunocytochemistry of the rat brain. Each subunit protein was differentially distributed even in the region like cerebellar cortex where mRNAs of both subunits were distributed in the same manner. This may indicate that GABAA receptor gamma 1 and gamma 2 subunit proteins are subject to a subunit-specific subcellular sorting mechanism.
Araki
T
T
Department of Anatomy and Neuroscience, Osaka University Medical School, Japan.
Kiyama
H
H
Maeno
H
H
Tohyama
M
M
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D009928:Organ Specificity; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D018079:Receptors, GABA / Q000032:analysis* / Q000276:immunology
D007106:Immune Sera; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D018079:Receptors, GABA