title: A high-capacity manifold support for the detection of specific IgE antibodies in allergic individuals.
abstract: A high-capacity manifold support with immobilized antigen was developed for the analysis of IgE-mediated immune reactivity in allergic subjects. Using this 96-pronged support, specific antibodies were trapped and detected from large sets of serum samples. We describe the binding of large amounts of antigen onto the expanded surface of the manifold support, permitting efficient identification of allergic individuals.
authors.affiliation: Beijer Laboratory, Department of Medical Genetics, Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Kwiatkowski
authors.forename: J
authors.initials: J
authors.lastname: Parik
authors.forename: U
authors.initials: U
authors.lastname: Landegren
mesh_terms: D000485:Allergens / Q000276:immunology*; D000918:Antibody Specificity; D002614:Chelating Agents; D004797:Enzyme-Linked Immunosorbent Assay; D005063:Europium; D005453:Fluorescence; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*
chemical_list: D000485:Allergens; D002614:Chelating Agents; D007073:Immunoglobulin E; D005063:Europium