Avidin-biotin based electrochemical immunoassay for thyrotropin.
A two site electrochemical enzyme immunoassay for thyrotropin (TSH) has been developed. This assay is based on the use of an immobilized capture antibody and a biotinylated second antibody. Detection is achieved via avidin labelled with alkaline phosphatase. The substrate 1-naphthyl phosphate was used and the product 1-naphthol was detected at disposable screen-printed carbon 8-electrode combs using specially designed instrumentation.
Athey
D
D
Department of Clinical Biochemistry, Medical School, University of Newcastle Upon Tyne, UK.
Ball
M
M
McNeil
C J
CJ
D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D004563:Electrochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013972:Thyrotropin / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D013972:Thyrotropin; D000469:Alkaline Phosphatase