title: Search for Mycobacterium paratuberculosis DNA in tissue from patients with sarcoidosis by enzymatic gene amplification.
abstract: The etiology of sarcoidosis has not yet been established, but several mycobacterial species have been implicated in the pathogenesis of this disease. We have used a nested primer polymerase chain reaction (PCR) specific for the IS900 Mycobacterium paratuberculosis insertion element to analyse paraffin-embedded mediastinal lymphoid tissue from 18 patients with pulmonary sarcoidosis verified by mediastinoscopy. Only the positive control contained M. paratuberculosis-specific DNA. The present study does not support a role for M. paratuberculosis in the pathogenesis of sarcoidosis. However, further studies are needed in order to clarify the possible role of other mycobacteria in this disease.
authors.affiliation: Department of Clinical Microbiology, Herlev Hospital, Denmark.
authors.forename: G
authors.initials: G
authors.lastname: Lisby
authors.forename: N
authors.initials: N
authors.lastname: Milman
authors.forename: G K
authors.initials: GK
authors.lastname: Jacobsen
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016927:Mycobacterium avium subsp. paratuberculosis; D016133:Polymerase Chain Reaction; D012507:Sarcoidosis / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial