Lactoferrin co-purifies with myeloperoxidase and is recognised by anti-neutrophil cytoplasm antibodies.
Thirty-five sera with binding greater than 20% in a myeloperoxidase (MPO, Calbiochem) ELISA were tested in Western blot analyses. 5/35 blotted MPO, but 5/35 blotted lactoferrin (LF) contaminating the commercial MPO preparation. All the anti-LF positive sera, but none of the anti-MPO positive sera, also exhibited anti-nuclear binding on Hep2 cells. Three of the patients with anti-LF antibodies had vasculitis affecting areas additional to the pulmonary-renal involvement which characterised the patients with anti-MPO antibodies.
Esnault
V L
VL
Service de Néphrologie-Immunologie Clinique, CHRU Nantes, France.
Short
A K
AK
Jones
S J
SJ
Skehel
M
M
Walker
J
J
Lockwood
C M
CM
D019268:Antibodies, Antineutrophil Cytoplasmic; D000974:Antibodies, Antinuclear / Q000276:immunology; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western; D003488:Cyanogen Bromide; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007781:Lactoferrin / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic; D009195:Peroxidase / Q000276:immunology*; D017421:Sequence Analysis; D017385:Sequence Homology; D014657:Vasculitis / Q000276:immunology*
D019268:Antibodies, Antineutrophil Cytoplasmic; D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D009195:Peroxidase; D007781:Lactoferrin; D003488:Cyanogen Bromide