Identification of a short viral transcript in Leishmania RNA virus-infected cells.
Certain strains of Leishmania guyanensis carry persistently infecting double-stranded RNA viruses. The viral polymerase has been shown to have both transcriptase and replicase activity. To date, only full-length RNA transcription of minus and plus strands have been reported. This report describes the synthesis of a 320 nt transcript which is complementary to the 3' end of the minus strand RNA. This plus-stranded short transcript was first detected in an in vitro polymerase assay. It was also detected in virus-infected Leishmania cells by a reverse transcription-polymerase chain reaction assay and by Northern analysis of infected Leishmania cell RNA.
Chung
I K
IK
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Armstrong
T C
TC
Patterson
J L
JL
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D012321:DNA-Directed RNA Polymerases; D018334:Leishmania guyanensis / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012328:RNA Viruses / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012321:DNA-Directed RNA Polymerases