title: Human liver 6-pyruvoyl-tetrahydropterin synthase: expression of the cDNA, purification and preliminary characterization of the recombinant protein.
authors.affiliation: Division of Clinical Chemistry, University Children's Hospital, Zürich, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Thöny
authors.forename: W
authors.initials: W
authors.lastname: Leimbacher
authors.forename: N
authors.initials: N
authors.lastname: Blau
authors.forename: C W
authors.initials: CW
authors.lastname: Heizmann
authors.forename: D
authors.initials: D
authors.lastname: Bürgisser
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D001483:Base Sequence; D002850:Chromatography, Gel; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006801:Humans; D007525:Isoelectric Focusing; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D019761:Phosphorus-Oxygen Lyases; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; D019761:Phosphorus-Oxygen Lyases; C043497:6-pyruvoyltetrahydropterin synthase