Induction of plasmacytomas that secrete monoclonal anti-peptide antibodies by retroviral transformation.
ABL-MYC, a retrovirus that coexpresses v-abl and c-myc, was used to infect six BALB/c mice that had been immunized twice with a KLH-conjugated peptide that consisted of the 18 carboxyterminal amino acids of protein kinase C-eta (PKC-eta). All mice developed transplantable, monoclonal plasmacytomas, and five out of six plasmacytomas secreted antigen-specific antibodies, even after transplantation. All these antibodies recognized PKC-eta on Western blots of crude cell lysates and did not cross react with other isoforms of the PKC family.
Weissinger
E M
EM
Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Henderson
D W
DW
Mischak
H
H
Goodnight
J
J
Mushinski
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000276:immunology; D002472:Cell Transformation, Viral / Q000235:genetics / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007527:Isoenzymes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000276:immunology; D016374:Oncogene Proteins v-abl / Q000235:genetics; D010954:Plasmacytoma / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D011493:Protein Kinase C / Q000276:immunology*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012190:Retroviridae / Q000235:genetics
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D016374:Oncogene Proteins v-abl; D016271:Proto-Oncogene Proteins c-myc; D006433:Hemocyanins; D011493:Protein Kinase C; C032808:keyhole-limpet hemocyanin