In vitro synthesized immunoglobulin A from nu/+ and reconstituted nu/nu mice against a dominant surface antigen of Giardia lamblia.
Nu/+ mice (ZU.ICR-strain) experimentally infected with Giardia lamblia (clone GS/M-83-H7) cleared the infection by day 45 postinfection (p.i.). Athymic nu/nu mice were reconstituted with immune Peyer's patch lymphocytes obtained from self-healed nu/+ littermates and thus acquired the potential to decrease their intestinal parasite mass. Intestinal B-cells from self-healed nu/+ mice as well as from immune-reconstituted athymic nude mice synthesized in vitro parasite-specific immunoglobulin A (IgA). This IgA was subsequently analyzed by immunoblotting, showing a predominant reaction with the major surface antigen (a 72,000-Da polypeptide) characterizing the Giardia clone in question. The hypothesis on the causative role of intestinal IgA and immune lymphocytes in the control of G. lamblia infection thus deserves further attention.
Gottstein
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B
Institute of Parasitology, University of Berne, Switzerland.
Deplazes
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P
Tanner
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I
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000276:immunology / Q000637:transplantation; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D016829:Giardia lamblia / Q000276:immunology*; D005873:Giardiasis / Q000276:immunology* / Q000628:therapy; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000276:immunology*; D007070:Immunoglobulin A / Q000096:biosynthesis*; D016219:Immunotherapy, Adoptive; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D010581:Peyer's Patches / Q000166:cytology
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016056:Immunodominant Epitopes; D007070:Immunoglobulin A