Metabolic profiling of amniotic fluid by proton nuclear magnetic resonance spectroscopy: correlation with fetal maturation and other clinical variables.
Proton NMR spectroscopy at 600 MHz was performed on 70 specimens of human amniotic fluid, representing pregnancies at different stages of maturation and with different fetomaternal complications. Spectra with good signal-to-noise characteristics were obtained in 3.5 min of acquisition time, directly on untreated fluid, with presaturation to suppress the intense water resonance. Metabolites that generally gave rise to well-resolved resonances included lactate, glucose, creatinine, choline derivatives, citrate, acetate, alanine, glycine, glutamate, succinate, and others. For three of these metabolites--creatinine, glucose, and lactate--the peak intensities correlated well with results of conventional chemical analysis. The NMR spectra of third-trimester specimens were readily distinguishable from those of second-trimester specimens, and several peak intensities correlated with fetal maturation during the third trimester. Significant correlations with maternal preeclampsia and fetal open spina bifida were also observed.
Bock
J L
JL
Department of Pathology, State University of New York at Stony Brook 11794-7300.
D000596:Amino Acids / Q000032:analysis; D000653:Amniotic Fluid / Q000737:chemistry*; D002794:Choline / Q000032:analysis; D003404:Creatinine / Q000032:analysis; D004314:Down Syndrome / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005260:Female; D005325:Fetal Organ Maturity; D005998:Glycine / Q000032:analysis; D006801:Humans; D007773:Lactates / Q000032:analysis; D019344:Lactic Acid; D008168:Lung / Q000196:embryology; D009682:Magnetic Resonance Spectroscopy; D011225:Pre-Eclampsia / Q000378:metabolism; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000378:metabolism; D016135:Spinal Dysraphism / Q000378:metabolism
D000596:Amino Acids; D007773:Lactates; D019344:Lactic Acid; D003404:Creatinine; D002794:Choline; D005998:Glycine