title: Carbon disulphide induced sensitivity changes of rat anococcygeus muscle to noradrenaline (NA).
abstract: The sensitivity of rat anococcygeus muscle to noradrenaline (NA) was reduced following carbon disulphide (CS2) pretreatment. Preparations incubated in excess Ca++ (4.2 mmol.L-1) or Zero Ca++ (0.0 mmol.L-1) media showed decrease in sensitivity to NA. Reserpinization failed to induce supersensitivity to NA in any Mg++ concentration in the media. Reserpinized tissue in Mg++ free media with EDTA showed increased sensitivity while decreased sensitivity and maximum response to NA in Mg++ (1.2 mmol.L-1) and excess Mg++ (3.6m mol.L-1) concentration of the medium. In the presence of EDTA, the inhibition observed with CS2 in excess Mg++ (3.6m mol.L-1) medium was comparatively much less in both non-reserpinized and reserpinized preparations. In normal Ca++ and its deficient medium decrease in sensitivity to CS2 and the relevant maximum response to NA were observed. In excess Ca++, the CS2 effect depressed corresponding to its maximal response to NA. Thus CS2 may be inhibiting responses to NA by interfering with Ca++ utilization.
authors.affiliation: Department of Neurobehavioural Toxicology, National Institute of Occupational Health (ICMR), Meghani Nagar, Ahmedabad, India.
authors.forename: D N
authors.initials: DN
authors.lastname: Gandhi
authors.forename: H
authors.initials: H
authors.lastname: Venkatakrishna-Bhatt
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002246:Carbon Disulfide / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008784:Methysergide / Q000494:pharmacology; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D009638:Norepinephrine / Q000037:antagonists & inhibitors*; D010646:Phentolamine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012110:Reserpine / Q000494:pharmacology; D013035:Spasm / Q000139:chemically induced
chemical_list: D003470:Culture Media; D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; D012110:Reserpine; D004492:Edetic Acid; D008274:Magnesium; D002246:Carbon Disulfide; D002118:Calcium; D009638:Norepinephrine; D008784:Methysergide; D010646:Phentolamine