High performance liquid chromatography of tamoxifen and metabolites in plasma and tissues.
An isocratic reversed-phase high performance liquid chromatographic method for the determination of tamoxifen and its metabolites in plasma and tissues is described. Plasma or tissue homogenate was extracted with methanol/dimethyl sulphoxide (4:1 v/v). The supernatant after centrifugation was separated on a BDS-Hypersil column with methanol/0.5 M ammonium acetate (75:25 v/v) as the mobile phase. The recoveries of tamoxifen added to plasma and liver tissue homogenate by the extraction procedure were 102 +/- 1.6 and 98 +/- 2.4% (mean +/- SD, n = 6), respectively. The solutes were detected at 280 nm with a detection limit of 0.25 micrograms/mL for tamoxifen.
Lim
C K
CK
MRC Toxicology Unit, University of Leicester, UK.
Chow
L C
LC
Yuan
Z X
ZX
Smith
L L
LL
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D013629:Tamoxifen / Q000032:analysis* / Q000097:blood
D007202:Indicators and Reagents; D013629:Tamoxifen