title: Determination of catecholamines in urine and plasma by on-line sample pretreatment using an internal surface boronic acid gel.
abstract: An automated method of analysis of catecholamines using a new packing material, internal surface boronic acid gel, was developed. The new support is designed with a carboxymethylcellulose-bonded external surface in order to be non-adsorptive to proteins and with a phenylboronic acid-bonded internal surface to retain only catecholamines. This packing support displayed an affinity for basic or neutral catecholic compounds with no protein adsorption and enabled on-line sample pretreatment of catecholamines in urine and deproteinized plasma. The catecholamines were selectively adsorbed on the new material and separated on a reversed-phase or a cation-exchange column. These compounds were then detected electrochemically. The limits of quantitation were 1.5-3.0 ng/ml in urine and 10-15 pg/ml in plasma, at a signal-to-noise ratio of 5.
authors.affiliation: Yokogawa Analytical Systems, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Soga
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D001897:Boronic Acids; D002395:Catecholamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004837:Epinephrine / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D009638:Norepinephrine / Q000032:analysis / Q000097:blood / Q000652:urine
chemical_list: D001897:Boronic Acids; D002395:Catecholamines; D009638:Norepinephrine; D004837:Epinephrine