title: [Detection of HIV-1 proviral DNA in peripheral leukocytes by AMPLICOR HIV-1 test kit].
abstract: AMPLICOR HIV-1 test kits, which had been developed as an HIV-1 provirus detection test by PCR method, have been evaluated for its clinical diagnostic application. Sixty-six of HIV-1 antibody positive and 67 of HIV-1 antibody negative blood samples derived from hemophiliacs, who had received blood products, have been tested by AMPLICOR HIV-1. All of the results from AMPLICOR HIV-1 were consistent with those from antibody test and clinical aspects. Thirty-nine of HIV-1 antibody positive samples have been tested by AMPLICOR HIV-1 and virus isolation (culture method). Twelve of 39 (30.8%) were positive by virus isolation, and 39 of 39 (100%) were positive by AMPLICOR HIV-1. Two of new born infants from HIV-1 sero-positive mothers were tested by AMPLICOR HIV-1, and the result suggested that the kit would be useful for diagnosis of infants from sero-positive mothers. Based on these studies, AMPLICOR HIV-1 is considered as useful clinical diagnostic for HIV-1 proviral DNA detection.
authors.affiliation: AIDS Research Center, National Institute of Health, Shinjuku-ku, Tokyo.
authors.forename: N
authors.initials: N
authors.lastname: Yoshihara
authors.forename: K
authors.initials: K
authors.lastname: Fukushima
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Honda
authors.forename: S
authors.initials: S
authors.lastname: Yamazaki
authors.forename: K
authors.initials: K
authors.lastname: Fukutake
authors.forename: T
authors.initials: T
authors.lastname: Meguro
authors.forename: K
authors.initials: K
authors.lastname: Yamada
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005260:Female; D015658:HIV Infections / Q000175:diagnosis; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007231:Infant, Newborn; D007962:Leukocytes / Q000382:microbiology*; D008297:Male; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic / Q000592:standards*
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic