title: [Subtractive hybridization--a technique for extracting DNA sequences, discriminating between two closely-related genomes].
abstract: This review deals with an analysis of the current status of genomic DNA subtraction. The theoretical calculations of kinetics of different ways of hybridization are implemented. The main conclusion is that the most preferable strategy for such a subtraction is hybridization of single stranded tracer with single-stranded driver DNA.
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D016678:Genome; D007700:Kinetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA
vernacular_title: Vychitaiushchaia gibridizatsiia--tekhnika ékstraktsii posledovatel'nosteĭ DNK, otlichaiushchikh dva blizkorodstvennykh genoma.