A novel cDNA corresponding to transcripts expressed in retina post-mitotic neurons.
The long term objective of this study is to isolate genes specifically expressed at the onset of neuronal cell cycle withdrawal. As an experimental paradigm we have used a quail neuroretinal cell clone (clone K2) immortalized by a thermosensitive mutant of Rous Sarcoma Virus. K2 cells proliferate at 36 degrees C but stop synthesizing DNA after a shift to 41.5 degrees C. We have constructed a cDNA library from K2 cells transferred to 41.5 degrees C and autosubtracted with RNAs from K2 cells maintained at 36 degrees C. This strategy has led to the isolation of cDNAs which recognize mRNAs expressed in quail neuroretina (NR) during development. We report here one of these cDNAs, cDNA QN1, that hybridizes with transcripts expressed in retina neurons, in parallel with their withdrawal from the cell cycle. QN1 ORF codes for a 138 kDa polypeptide corresponding to the protein observed in Western blot analysis. A role of QN1 product(s) on neuronal quiescence is suggested by the positive effect of an antisense oligonucleotide on DNA synthesis of K2 cells.
Bidou
L
L
Centre de Biologie Cellulaire, Ivry, France.
Crisanti
P
P
Blancher
C
C
Pessac
B
B
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D002478:Cells, Cultured; D002999:Clone Cells; D003370:Coturnix; D018076:DNA, Complementary / Q000235:genetics*; D017403:In Situ Hybridization; D007700:Kinetics; D008938:Mitosis; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012160:Retina / Q000166:cytology / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger