Construction and characterization of a YAC library with a low frequency of chimeric clones from flow-sorted human chromosome 9.
Human chromosome 9 DNA, flow-sorted from somatic cell hybrid PK-87-9, has been used to construct two complete digest YAC libraries. The combined representation of chromosome 9 in these libraries, estimated by hybridization of chromosome 9-specific sequences to YAC colony grids, is approximately 95%. The frequency of chimeric clones, analyzed by fluorescence in situ hybridization of chromosome 9 YACs to human metaphase chromosomes, was estimated to be approximately 4%. These libraries provide a resource for physical mapping and for moving from genetic markers to disease loci on chromosome 9.
McCormick
M K
MK
Center for Human Genome Studies and Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Buckler
A
A
Bruno
W
W
Campbell
E
E
Shera
K
K
Torney
D
D
Deaven
L
L
Moyzis
R
R
D002678:Chimera; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005091:Exons; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D015183:Restriction Mapping
D004247:DNA