title: Biologically active two-dimensional crystals of aquaporin CHIP.
abstract: Plasma membranes of several mammalian tissues are highly permeable to water due to the presence of CHIP, the 28-kDa channel-forming integral protein which is the archetypal member of the aquaporin family of water channel proteins. To define its native structure, purified red cell CHIP protein was reconstituted into lipid bilayers at a high protein-to-lipid ratio, and the resulting 3-microns diameter membrane vesicles were examined by high resolution electron microscopy. The reconstituted membranes contained highly ordered two-dimensional crystalline lattices of p422(1) symmetry in which each CHIP tetramer contained a central depression extending from the outer and inner surfaces of the membrane into the transbilayer domain of the molecule. The reconstituted membranes also exhibited extremely high osmotic water permeability, Pf = 0.472 cm/s, corresponding to the sum of activities of all incorporated CHIP molecules. These studies report the first two-dimensional crystallization of a biologically active water channel and provide direct evidence of the structure responsible for its pore-like behavior.
authors.affiliation: M. E. Müller Institute for High Resolution Electron Microscopy, University of Basel, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Walz
authors.forename: B L
authors.initials: BL
authors.lastname: Smith
authors.forename: M L
authors.initials: ML
authors.lastname: Zeidel
authors.forename: A
authors.initials: A
authors.lastname: Engel
authors.forename: P
authors.initials: P
authors.lastname: Agre
mesh_terms: D051398:Aquaporin 1; D020346:Aquaporins; D001789:Blood Group Antigens; D002151:Calorimetry; D002463:Cell Membrane Permeability; D003460:Crystallization; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000097:blood / Q000737:chemistry* / Q000648:ultrastructure; D008854:Microscopy, Electron; D017348:Microscopy, Electron, Scanning Transmission
chemical_list: C496020:AQP1 protein, human; D020346:Aquaporins; D001789:Blood Group Antigens; D008565:Membrane Proteins; D051398:Aquaporin 1