Identification of a small RNA that interacts with the 5' splice site of the Trypanosoma brucei spliced leader RNA in vivo.
In vivo psoralen cross-linking of the trypanosome spliced leader (SL) RNA has led to the discovery of a small RNA that we provisionally call the spliced leader-associated (SLA) RNA. The 72 nt SLA RNA is unlike any known small RNA except for a small region that resembles U5 snRNA. The SL/SLA RNA cross-links map to two regions, the predominant interactions occurring between the 5' splice site region of the SL RNA and a CUUUUA sequence in the SLA RNA. The resemblance between these cross-links and interactions of U5 snRNA with cis-spliced pre-mRNAs suggests that the SLA RNA may be the trans-splicing analog of U5 snRNA in trypanosomes.
Watkins
K P
KP
University of California, San Francisco.
Dungan
J M
JM
Agabian
N
N
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D012322:RNA Precursors / Q000378:metabolism; D015347:RNA Probes; D012326:RNA Splicing; D016053:RNA, Protozoan / Q000302:isolation & purification / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000302:isolation & purification / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000378:metabolism*
D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D012322:RNA Precursors; D015347:RNA Probes; D016053:RNA, Protozoan; D012342:RNA, Small Nuclear