8-methoxypsoralen plus UVA induces the 72 kDa heat shock protein in organ-cultured normal human skin.
The proteins induced by heat and other stressors, called heat shock proteins (HSP) or stress proteins, are considered to play a general role in protection from cellular injury. Exposure to UVA (320-400 nm) following application of 8-methoxypsoralen (8-MOP), termed PUVA is commonly used in the field of dermatology. In order to understand the induction of HSP in PUVA-treated human skin, indirect immunofluorescence using a monoclonal antibody specific for the 72 kDa HSP (HSP 72) was carried out in organ-cultured normal human skin that was treated with PUVA. When the organ-cultured skin was treated at 37 degrees for 1 h with 8-MOP at a final concentration of 10 or 100 micrograms/mL and exposed to UVA (51.3 kJ/m2), nuclear immunofluorescence of HSP 72 was detected in the epidermal cells 12 h after UVA irradiation. In contrast, the induction of HSP 72 was not detected either by UVA irradiation or 8-MOP treatment. These results suggest that PUVA treatment is one of the stressors for human skin, and DNA damage caused by PUVA induces HSP 72.
Muramatsu
T
T
Department of Dermatology, Nara Medical University, Japan.
Yamashina
Y
Y
Tada
H
H
Kobayashi
N
N
Yamaji
M
M
Ohno
H
H
Shirai
T
T
Takahashi
A
A
Ohnishi
T
T
D000368:Aged; D005260:Female; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008730:Methoxsalen / Q000494:pharmacology*; D009924:Organ Culture Techniques; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D006360:Heat-Shock Proteins; D008730:Methoxsalen