Migration patterns of lymphocytes following syngeneic heterotopic small bowel transplantation in rodents.
Adult rats received syngeneic accessory small bowel grafts with venous drainage to either the portal vein (hetero-portal) or the inferior vena cava (hetero-systemic). Lymphoid cell recovery in different lymphoid organs (spleen, pooled peripheral lymph nodes, mesenteric nodes, Peyer's Patches) was evaluated at varying times (days 0-300) post-grafting. While minimal changes were observed for cell recovery in other organ tissues, lymphocyte recovery in Peyer's Patches of both host and graft small intestine of hetero-systemic animals was decreased from 10- to 100-fold with respect to hetero-portal recipients or non-operated controls. These changes were seen throughout the time course of the study. In additional experiments, lymphoid cells from different organs/donors were labelled in vitro with 111In and injected intravenously into normal/transplanted recipients. Recovery of 111In in various organs was assessed at 1 and 6 h postinjection. The major change seen was in the decreased ability of mononuclear cells derived from Peyer's Patches to migrate to small intestinal tissue (host and graft) in hetero-systemic recipients. In addition, Peyer's Patch cells from these animals 'homed' poorly to small intestine in non-operated animals by comparison with cells from normal rats (or hetero-portal donors).
Koh
I
I
Department of Surgery, University of Toronto, Ont., Canada.
Cohen
Z
Z
Levy
G
G
Plapler
H
H
Wojcik
D
D
Gorczynski
R M
RM
D000818:Animals; D002452:Cell Count; D002465:Cell Movement; D005260:Female; D006085:Graft Survival / Q000276:immunology; D007421:Intestine, Small / Q000098:blood supply / Q000276:immunology / Q000637:transplantation*; D008214:Lymphocytes / Q000166:cytology*; D008221:Lymphoid Tissue / Q000166:cytology; D010581:Peyer's Patches / Q000166:cytology; D011169:Portal Vein; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016042:Transplantation, Heterotopic / Q000276:immunology*; D014185:Transplantation, Isogeneic; D014682:Vena Cava, Inferior