The DNA polymerase-encoding gene of African swine fever virus: sequence and transcriptional analysis.
The putative DNA polymerase-encoding gene of African swine fever virus has been sequenced. The gene, designated G1207R, is located in the central region of the viral genome, and encodes a protein of 1207 amino acids (aa) with a predicted M(r) of 139,835. The gene is transcribed at both early and late stages of infection into a 4.1-kb RNA. Transcription is initiated at tsp, 8 nucleotides (nt) upstream from the start codon. Open reading frame (ORF) G1207R contains four direct repeats in tandem close to the 3'-end. Each repeat consists of 12 nt, coding for the reiterated sequence, K/NPAG. The deduced aa sequence of G1207R shows significant similarity with DNA polymerases from cellular and viral origin, belonging to the alpha-like family of DNA polymerases. In particular, the G1207R protein presents a colinear arrangement of all the 3'-->5' exonuclease and polymerization highly conserved aa regions characteristic of this group of DNA-dependent DNA polymerases.
Rodríguez
J M
JM
Centro de Biologia Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Yáñez
R J
RJ
Rodríguez
J F
JF
Viñuela
E
E
Salas
M L
ML
D000358:African Swine Fever Virus / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase