title: conformation and phasing of dystrophin structural repeats.
abstract: The presumptive rod domain of dystrophin contains a series of degenerate repeating sequences with homology to those of spectrin. To determine the relation of the implied structural repeating units to the sequence repeat (the phasing), recombinant fragments of the domain of dystrophin were prepared by expression in Escherichia coli. The phasing was established by identifying the minimum sequence element that would form a stable fold of high (approx. 75%) alpha-helicity: by contrast, incorrectly phased fragments had labile structure with an average alpha-helicity of about 40%. The isolated folded structural repeat showed high stability towards proteolysis and a urea-denaturation profile with a plateau at low denaturant concentration, indicative of a unique folded conformation. The phasing is consistent with a structure inferred from analysis of the amino acid sequence and also found in spectrin, in which each structural repeat comprises a three-stranded coiled-coil, made up of one short helix (approx. 30 residues) and the N and C-terminal halves of two separate long helices, such that each long helix participates in the formation of two contiguous structural units.
authors.affiliation: Medical Research Council Muscle and Cell Motility Unit, King's College, London.
authors.forename: E
authors.initials: E
authors.lastname: Kahana
authors.forename: P J
authors.initials: PJ
authors.lastname: Marsh
authors.forename: A J
authors.initials: AJ
authors.lastname: Henry
authors.forename: M
authors.initials: M
authors.lastname: Way
authors.forename: W B
authors.initials: WB
authors.lastname: Gratzer
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D016189:Dystrophin / Q000737:chemistry* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006801:Humans; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013049:Spectrin / Q000737:chemistry
chemical_list: D016189:Dystrophin; D011994:Recombinant Proteins; D013049:Spectrin