title: Genetic analysis of the regulatory putP region (coding for proline permease) in Klebsiella pneumoniae M5a1: evidence for regulation by the nac system.
abstract: During a search for nitrogen-controlled promoters on the Klebsiella pneumoniae M5a1 chromosome, the regulatory region of putP (coding for proline permease) was cloned, sequenced and analyzed. The region contained a weak sigma 70-dependent promoter and putative binding sites for the cAMP-CAP complex and the Nac regulatory protein, the latter probably providing a link with the nitrogen regulation (Ntr) system. Using a lacZ gene fusion, evidence for control of putP transcription by both Nac and Ntr was obtained.
authors.affiliation: Lehrstuhl für Mikrobiologie der Universität, Bayreuth, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Suhr
authors.forename: D
authors.initials: D
authors.lastname: Kleiner
mesh_terms: D000595:Amino Acid Sequence; D026921:Amino Acid Transport Systems, Neutral; D001483:Base Sequence; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D026901:Membrane Transport Proteins; C033082:proline transporter