Detection of H. pylori in saliva using a monoclonal antibody.
The presence of Helicobacter pylori in saliva specimens collected from infected children examined before and after specific therapy and from a non-infected pediatric population was detected by indirect immunofluorescence assay (IIF) using a specific monoclonal antibody. Among the 25 children with H. pylori-negative antral biopsies, 4 had in their salivas bacterial cells similar to H. pylori, but a retrospectively performed serologic test showed a positive response. No false-positive reaction was observed among non-infected and seronegative children. The observation of bacterial cells with H. pylori morphology in saliva by IIF was consistent with the presence of this bacterium in antral biopsies. A person-to-person transmission of H. pylori by saliva thus appeared to be possible.
Husson
M O
MO
Laboratoire de Bactériologie A, Lille, France.
Gottrand
F
F
Turck
D
D
Leclerc
H
H
D000293:Adolescent; D000328:Adult; D000658:Amoxicillin / Q000627:therapeutic use; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001729:Bismuth / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D005455:Fluorescent Antibody Technique; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000188:drug therapy; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D009061:Mouth Mucosa / Q000382:microbiology; D012463:Saliva / Q000382:microbiology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000658:Amoxicillin; D001729:Bismuth