title: Assignment of disulfide bonds in corticotropin-releasing factor-binding protein.
abstract: We have previously isolated, cloned, and characterized a protein that specifically binds and inactivates the peptide corticotropin-releasing factor. The integrity of the disulfide bonds in the binding protein is essential for this activity as reduction abolishes the protein's ability to bind corticotropin-releasing factor. The disulfide arrangement of the 10 cysteines present in the mature protein was established by analysis of proteolytically cleaved protein and sequence analysis of cystine containing fragments. A pattern is observed where each cysteine is connected to the next one in a sequential manner. Inspection of the genomic DNA encoding for this protein reveals that four of the domains defined by disulfide linkage coincide with four different exons.
authors.affiliation: Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037.
authors.forename: W H
authors.initials: WH
authors.lastname: Fischer
authors.forename: D P
authors.initials: DP
authors.lastname: Behan
authors.forename: M
authors.initials: M
authors.lastname: Park
authors.forename: E
authors.initials: E
authors.lastname: Potter
authors.forename: P J
authors.initials: PJ
authors.lastname: Lowry
authors.forename: W
authors.initials: W
authors.lastname: Vale
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D011994:Recombinant Proteins; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D004220:Disulfides; D010446:Peptide Fragments; D011994:Recombinant Proteins; C067133:corticotropin releasing factor-binding protein