Optimization of culture conditions for feline x murine heterohybridomas.
Feline splenocytes were fused to the murine myeloma lines NSO or Ag8. Autologous serum and taurine were used as media supplements for the cat x mouse heterohybridomas. The best results were obtained by the use of NSO as fusion line with taurine-supported media.
Leidinger
E
E
Institute of Medical Chemistry (Peptide and Protein Group), University of Veterinary Sciences, Vienna, Austria.
Kramberger-Kaplan
E
E
Gemeiner
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002415:Cats; D002455:Cell Division; D002459:Cell Fusion; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D005615:Freezing; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007621:Karyotyping; D008677:Metaphase; D051379:Mice; D013154:Spleen / Q000166:cytology; D013654:Taurine; D013997:Time Factors
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013654:Taurine