Human monoclonal lambda light chain protein exhibits specific binding to the variable region of monoclonal anti-IgE antibody.
A monoclonal heterohybridoma cell line (B11-12) was developed by fusing EBV-transformed lymphocytes from a patient with hyper-IgE syndrome and SHM-D33 heteromyeloma cells. The resultant heterohybridoma secreted only human lambda L chains exhibiting specific binding to mouse monoclonal Ab to human IgE (mAb-algE). B11-12 bound to four separate mAb-algE, but not to mAb of the same isotypes directed to other antigens. The mAb-algE differed in isotype (IgG1; IgG2b) demonstrating that B11-12 was recognizing an idiotypic, but not an isotypic determinant on the mAb-algE. Human IgE did not inhibit B11-12 binding to mAb-algE, suggesting the interaction between B11-12 and mAb-algE involved an idiotope outside the combining site of the mAb-algE. Human sera, including serum from the donor of the lymphocytes used to produce heterohybridoma B11-12, demonstrated the capacity to inhibit B11-12 binding to mAb-algE. This, along with the inability of these same sera to directly bind B11-12, suggests that the serum contained reactivity similar to B11-12. We propose that B11-12 demonstrates specificity resembling an anti-Id to anti-human IgE Ab.
Ludwig
L B
LB
Department of Medicine, State University of New York at Buffalo.
Schwartz
S A
SA
Insel
R A
RA
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region / Q000378:metabolism*; D007146:Immunoglobulin lambda-Chains / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D011485:Protein Binding; D011487:Protein Conformation
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D007073:Immunoglobulin E