title: Anti-granulocyte perinuclear antibodies but not anti-neutrophil cytoplasmic antibodies (ANCA) in rheumatoid arthritis.
abstract: We studied 45 patients with rheumatoid arthritis for the presence of ANCA. These antibodies were determined by indirect immunofluorescence (IIF) and by enzyme-linked immunosorbent assays (ELISAs) using as a substrate purified myeloperoxidase and purified extract of azurophilic granules. By IIF, we found a characteristic perinuclear immunostaining pattern in 21 cases (47%). However, no patient had a positive result by the two ELISAs performed. Patients with a positive IIF result had significantly higher levels of anti-nuclear and anti-ds DNA antibodies than those with a negative IIF result. Therefore, these antibodies must correspond to the previously reported as granulocyte specific antinuclear antibodies (GS-ANA).
authors.affiliation: Service of Internal Medicine, Hospital Clínic i Provincial, Barcelona, Spain.
authors.forename: X
authors.initials: X
authors.lastname: Bosch
authors.forename: J
authors.initials: J
authors.lastname: Llena
authors.forename: J
authors.initials: J
authors.lastname: Font
authors.forename: A
authors.initials: A
authors.lastname: Collado
authors.forename: E
authors.initials: E
authors.lastname: Mirapeix
authors.forename: A
authors.initials: A
authors.lastname: Urbano-Márquez
authors.forename: M
authors.initials: M
authors.lastname: Ingelmo
mesh_terms: D000328:Adult; D000368:Aged; D019268:Antibodies, Antineutrophil Cytoplasmic; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D002467:Cell Nucleus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000648:ultrastructure*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D001323:Autoantibodies; D007074:Immunoglobulin G