title: Regulation of the expression of the H(+)-ATPase genes PMA1 and PMA2 during growth and effects of octanoic acid in Saccharomyces cerevisiae.
abstract: A peak of plasma membrane H(+)-ATPase activity during exponential growth is correlated with the expression of the PMA1 gene as monitored by measurements of the beta-galactosidase activity from a PMA1-lacZ fusion. This peak of activity is also correlated to the content of the H(+)-ATPase protein in yeast plasma membrane as shown by quantitative immunodetection. The PMA2-lacZ fusion assay indicates that the expression of the PMA2 gene is activated somewhat later during exponential phase but under all circumstances its activity remains at least 500-fold lower than that of the PMA1-lacZ fusion. A slight but significant stimulation of ATPase activity by low concentrations of octanoic acid coincides with a decrease in the PMA1 gene expression. It is concluded that octanoic acid stimulates de PMA1 ATPase activity by posttranslational mechanisms.
authors.affiliation: Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisboa, Portugal.
authors.forename: C A
authors.initials: CA
authors.lastname: Viegas
authors.forename: P
authors.initials: P
authors.lastname: Supply
authors.forename: E
authors.initials: E
authors.lastname: Capieaux
authors.forename: L
authors.initials: L
authors.lastname: Van Dyck
authors.forename: A
authors.initials: A
authors.lastname: Goffeau
authors.forename: I
authors.initials: I
authors.lastname: Sá-Correia
mesh_terms: D002210:Caprylates / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005800:Genes, Fungal; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
chemical_list: D002210:Caprylates; D006180:Proton-Translocating ATPases; C031492:octanoic acid