An alternative protocol for the serial passage and maintenance of a homogonic strain of Strongyloides ratti in female Sprague-Dawley rats.
A method which does not involve the tedious use of watch glass coprocultures for obtaining filariform infective (L3) larvae of Strongyloides ratti from faecal pellets of infected Sprague-Dawley rats is described. The alternative method utilises Baermannization (18 h) of faecal pellets to yield rhabditiform (L1) larvae of S. ratti and their subsequent culture for 72 h at 19 degrees C in tissue-culture-flasks containing only dechlorinated tap water to yield infective filariform (L3) larvae. The yields and infectivity of the L3 larvae obtained from the two methods were essentially similar.
Mendis
A H
AH
School of Biomedical Sciences, Curtin University of Technology, Perth, Western Australia.
Grubb
W B
WB
Armson
A
A
D000818:Animals; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000469:parasitology*; D012692:Serial Passage / Q000379:methods; D017170:Strongyloides ratti / Q000254:growth & development*