title: A novel gene delivery system using EGF receptor-mediated endocytosis.
abstract: A monoclonal antibody to the human epidermal growth factor (EGF) receptor was conjugated with polylysine and the resulting conjugate was affinity-linked with DNA (gene). This novel gene delivery system utilizes receptor-mediated endocytosis and would be especially suitable for gene therapy for EGF receptor-overproducing squamous cell carcinomas.
authors.affiliation: Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Gamou
authors.forename: A
authors.initials: A
authors.lastname: Takayanagi
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
mesh_terms: D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004247:DNA / Q000008:administration & dosage / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004705:Endocytosis; D066246:ErbB Receptors / Q000502:physiology*; D018014:Gene Transfer Techniques; D006801:Humans; D011107:Polylysine; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D011107:Polylysine; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D066246:ErbB Receptors