title: Effects of oestrogen on progesterone synthesis and arachidonic acid metabolism in human luteal cells.
abstract: OBJECTIVE
  Locally produced oestrogens and prostaglandins (PGs) are implicated in the regulation of luteal lifespan in the human ovary. This study (1) assesses direct effects of these factors on progesterone synthesis in isolated luteal cells, and (2) explores interactions between luteal age and treatment with gonadotrophin or oestrogen on the metabolism of arachidonic acid (prostaglandin precursor) by steroidogenic luteal cells in vitro.
  
  
  METHODS
  Primary monolayer cultures of human luteal cells obtained at different stages of the luteal phase were used to investigate the effect of oestradiol, catechol oestrogens (2- and 4-hydroxyoestradiol), diethylstilboestrol, PGE2 and PGF2 alpha on basal and human chorionic gonadotrophin (hCG) stimulated progesterone production in vitro. The role of PGs as modulators of luteal cell function was further investigated by studying the metabolic fate of radioactively labelled arachidonic acid in hormone treated (oestradiol and hCG) and control cultures, assessed by high performance liquid chromatography.
  
  
  METHODS
  Corpora lutea were enucleated from nine women with regular ovulatory cycles undergoing microsurgical reversal of tubal sterilization. Granulosa cell aspirates were obtained from three patients undergoing in-vitro fertilization treatment.
  
  
  RESULTS
  PGE2 and PGF2 alpha at various concentrations did not have a consistent effect, whereas oestradiol, diethylstilboestrol (and 2-hydroxyoestradiol in early luteal cell cultures) significantly inhibited basal and hCG stimulated progesterone biosynthesis. Evidence for direct inhibition of 3 beta-hydroxysteroid dehydrogenase enzymic activity by oestradiol was obtained. Both major metabolic pathways of arachidonic acid (lipoxygenase and cyclo-oxygenase) were operative in steroidogenic luteal cells recovered throughout the luteal phase. The ratio of PGE2 to PGF2 alpha synthesis in vitro by human luteal cells from endogenously incorporated arachidonic acid did not change significantly with corpus luteum age, with PGE2 tending to predominate. Oestradiol treatment shifted arachidonic acid metabolism from the lipoxygenase towards the cyclooxygenase pathway in cells isolated from ageing corpora lutea.
  
  
  CONCLUSIONS
  Oestradiol, at relatively high concentrations, is a potent inhibitor of basal and hCG induced luteal cell steroidogenesis in vitro. No support is provided for the concept that luteolysis is mediated by local production of PGF2 alpha. The putative luteolytic effect of oestradiol may entail reduced metabolism of arachidonic acid to lipoxygenase derived products by luteal cells rather than direct stimulation of prostaglandin production by itself.
authors.affiliation: Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
authors.forename: B
authors.initials: B
authors.lastname: Fisch
authors.forename: M P
authors.initials: MP
authors.lastname: Rose
authors.forename: M G
authors.initials: MG
authors.lastname: Elder
authors.forename: R M
authors.initials: RM
authors.lastname: Winston
authors.forename: R A
authors.initials: RA
authors.lastname: Margara
authors.forename: S G
authors.initials: SG
authors.lastname: Hillier
mesh_terms: D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003864:Depression, Chemical; D004054:Diethylstilbestrol / Q000494:pharmacology; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008183:Luteal Phase / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*
chemical_list: D001095:Arachidonic Acids; D006063:Chorionic Gonadotropin; D004967:Estrogens; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol; C001390:2-hydroxyestradiol; D015237:Dinoprost; D015232:Dinoprostone