A microtitre plate method for measuring biopterin with cryopreserved Crithidia fasciculata.
The assay of biopterin derivatives in dried blood spots is used by us in initial screening for inherited defects in tetrahydrobiopterin synthesis. The previously described method (1) required aseptic technique and microbiological facilities. The modification detailed here has the advantages of antibiotic cover, which overcomes these needs and microtitre plate technology allowing the incubation time to be halved with precision and accuracy retained. Data reduction facilities may be applied.
Leeming
R J
RJ
Department of Clinical Chemistry, Childrens Hospital, Ladywood Middleway, Birmingham, United Kingdom.
Hall
S K
SK
Friday
H
H
Hurley
P
P
Green
A
A
D000328:Adult; D000592:Amino Acid Metabolism, Inborn Errors / Q000097:blood / Q000175:diagnosis*; D000818:Animals; D001708:Biopterins / Q000097:blood*; D016831:Crithidia fasciculata / Q000254:growth & development*; D015925:Cryopreservation; D006801:Humans; D007202:Indicators and Reagents; D010651:Phenylalanine Hydroxylase / Q000172:deficiency*; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods
D007202:Indicators and Reagents; D001708:Biopterins; D010651:Phenylalanine Hydroxylase