title: Single-strand conformational polymorphism (SSCP) mapping of the mouse genome: integration of the SSCP, microsatellite, and gene maps of mouse chromosome 1.
abstract: Interspersed repetitive sequence (IRS) PCR and repetitive element-to-bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repetitive element-linked STSs to the mouse map: 22 to Chr 1, 10 to Chr. 15, 2 each to Chrs 12 and 14, and three to Chr 7. In addition, we have integrated the SSCP, single-strand length polymorphism, and restriction fragment length polymorphism maps of mouse Chr. 1, resulting in a high-density map of the chromosome, containing over 100 loci, all typed on a single interspecific backcross.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: K W
authors.initials: KW
authors.lastname: Hunter
authors.forename: M L
authors.initials: ML
authors.lastname: Watson
authors.forename: J
authors.initials: J
authors.lastname: Rochelle
authors.forename: S
authors.initials: S
authors.lastname: Ontiveros
authors.forename: D
authors.initials: D
authors.lastname: Munroe
authors.forename: M F
authors.initials: MF
authors.lastname: Seldin
authors.forename: D E
authors.initials: DE
authors.lastname: Housman
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D016678:Genome; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers