Concanavalin A-bound selenoprotein in human serum analyzed by graphite furnace atomic absorption spectrometry.
We developed an assay for the direct determination of selenium in serum with a Perkin-Elmer Model 4100ZL Zeeman atomic absorption spectrometer and Ag-Cu-Mg modifier. We used this assay to analyze concanavalin A-bound selenoprotein (CABSP) in human serum after concanavalin A (ConA) affinity chromatography. The CABSP was identified as a single-chain glycoprotein of 57.3 kDa. Carbohydrate units were N- and O-linked to the protein. The selenium moiety was selenocysteine. Total selenium, glutathione peroxidase (GPX; EC 1.11.1.9), ConA-bound selenium (CABS), and alpha 1-acid glycoprotein (AAG) were determined in normal subjects and patients with various pathological conditions. CABS accounted for 44.1% +/- 6.3% of total selenium in sera from normal subjects and 46.5% +/- 3.9% to 55.1% +/- 8.1% in sera from patients with a variety of diseases. Total selenium in serum was well correlated with serum CABS (r = 0.860), but not with serum GPX activity (r = 0.117), for all patients studied. Serum CABS increased in normal subjects after selenium supplementation. Serum CABSP did not behave as an acute-phase reactant, compared with AAG.
Daher
R
R
Department of Chemistry, Cleveland State University, OH 44115.
Van Lente
F
F
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003208:Concanavalin A / Q000378:metabolism*; D003920:Diabetes Mellitus / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006031:Glycosylation; D006801:Humans; D007674:Kidney Diseases / Q000097:blood; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood; D009961:Orosomucoid / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012016:Reference Values; D012643:Selenium / Q000008:administration & dosage / Q000097:blood*; D051140:Selenoproteins; D013054:Spectrophotometry, Atomic
D009961:Orosomucoid; D011506:Proteins; D051140:Selenoproteins; D003208:Concanavalin A; D005979:Glutathione Peroxidase; D012643:Selenium