5,6-Dihydrouridine: a marker ribonucleoside for determining whole body degradation rates of transfer RNA in man and rats.
It has previously been demonstrated that N6-threoninocarbonyladenosine is virtually quantitatively excreted in urine. From the similarity of the average molar ratio of 5,6-dihydrouridine to N6-threoninocarbonyladenosine in the urine of human adults (12.6), newborns (12.6) and rats (13.6) with the respective ratio in cytoplasmic tRNA (11.8) we conclude that 5,6-dihydrouridine is also virtually quantitatively excreted in urine. Therefore, excreted 5,6-dihydrouridine is suitable as a marker to assess the whole body degradation rate of tRNA. Relative degradation rates of tRNA determined via excreted 5,6-dihydrouridine in urine are 4.7 times higher in rats (2.2 +/- 0.33 mumol/kg per day) than in human adults (0.48 +/- 0.05 mumol/kg per day) which is similar to the respective difference in the resting metabolic rate per weight unit.
Topp
H
H
Research Institute of Child Nutrition, Dortmund, Germany.
Duden
R
R
Schöch
G
G
D000241:Adenosine / Q000031:analogs & derivatives / Q000652:urine; D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000652:urine; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005609:Free Radicals; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D012343:RNA, Transfer / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013045:Species Specificity; D014529:Uridine / Q000031:analogs & derivatives* / Q000652:urine
D015415:Biomarkers; D005609:Free Radicals; D017382:Reactive Oxygen Species; C003268:N(6)-(N-threonylcarbonyl)adenosine; C010922:5,6-dihydrouridine; D012343:RNA, Transfer; D000241:Adenosine; D014529:Uridine