title: Combined enzyme and antimicrobial susceptibility profiles of caprine Pasteurella haemolytica serovar 2 respiratory tract isolates.
abstract: The present investigation describes a novel method of demonstrating strain diversity among Pasteurella haemolytica biovar A, serovar 2 (PhA2) nasal turbinate isolates from a flock of 32 experimental goats during a naturally occurring outbreak of pasteurellosis. After a 21 day conditioning period in a feedyard, 51 PhA2 isolates from 27 culture-positive goats were identified including 1 on day 22, 14 on day 25, 21 on day 39, and 15 on day 66. Each PhA2 isolate was evaluated for its enzyme activity against 19 substrates with a commercial semiquantitative enzyme system and for its antimicrobial susceptibility with 12 drugs, resulting in 7 different enzyme profiles and 8 different antimicrobial susceptibility profiles. A total of 14 combined enzyme and antimicrobial susceptibility profiles were produced. The same PhA2 strain was isolated from only 4 of the 12 goats with 2 PhA2 isolations, while the same PhA2 strain was isolated from only 1 of the 6 goats with 3 PhA2 isolations. The data from this investigation demonstrated that the PhA2 upper respiratory tract flora from goats is highly heterologous.
authors.affiliation: Texas Veterinary Medical Center, Department of Veterinary Pathobiology, Texas A&M University, College Station 77843-4475.
authors.forename: C M
authors.initials: CM
authors.lastname: Scanlan
authors.forename: C W
authors.initials: CW
authors.lastname: Purdy
authors.forename: G S
authors.initials: GS
authors.lastname: Foster
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004196:Disease Outbreaks / Q000662:veterinary; D004352:Drug Resistance, Microbial; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology*; D006041:Goats; D008297:Male; D016978:Mannheimia haemolytica / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D010326:Pasteurella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D012703:Serotyping; D013781:Texas / Q000453:epidemiology; D014886:Weaning
chemical_list: D000900:Anti-Bacterial Agents