title: Cell-cycle-stage specificity of the methotrexate block as resolved by X-ray-induced chromosome damage.
abstract: Radiation-induced chromosome aberrations were used as biomarkers to compare G0, mid-G1, and methotrexate (MTX)-arrested lymphocytes. The ratio of chromosome-type to chromatid-type aberrations in MTX-arrested cells was consistent with that predicted when postreplicative chromosomes are exposed to ionizing radiation and supports the premise that MTX arrests cells in late S/G2 of the cell cycle.
authors.affiliation: Department of Biology, Tuskegee University, AL 36088.
authors.forename: V B
authors.initials: VB
authors.lastname: Richardson
authors.forename: L G
authors.initials: LG
authors.lastname: Littlefield
authors.forename: A M
authors.initials: AM
authors.lastname: Sayer
authors.forename: P T
authors.initials: PT
authors.lastname: Peterson
mesh_terms: D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D008727:Methotrexate / Q000494:pharmacology*
chemical_list: D008727:Methotrexate