150-kDa proteins in dog serum bind 1.5-kDa growth-promoting factors for androgen-independent canine prostatic epithelial cells.
Fractions obtained by gel filtration or ultrafiltration of dog serum were tested for their mitogenic activity on canine prostatic epithelial cells: two prostatic growth factor (PGF) entities were found, a major one of 150 kDa (PGF-I) and a minor one of 1.5-2.0 kDa (PGF-II). Treatment and/or extraction with acetic acid, hydrochloric acid, or acidified-ethanol or preparations enriched in PGF-I obtained either by ion-exchange chromatography, acetone precipitation, or retention by ultrafiltration membrane (cut-off 30 kDa) resulted, upon gel filtration, in the detection of a mitogenic activity eluting mainly at the position of PGF-II. Acid hydrolysis and proteolysis of PGF-II led to a loss of activity. It is proposed that, in dog serum, mitogenic peptides for prostatic epithelial cells of 1.5 kDa (PGF-II) are found in their free form and/or in association with proteins of 150 kDa (PGF-I).
Chevalier
S
S
Department of Medicine, Maisonneuve-Rosemont Research Center, Montreal, Quebec, Canada.
McKercher
G
G
Chapdelaine
A
A
D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division; D004285:Dogs; D004847:Epithelial Cells; D006133:Growth Substances / Q000097:blood* / Q000737:chemistry / Q000502:physiology; D006868:Hydrolysis; D008297:Male; D011467:Prostate / Q000166:cytology*
D001798:Blood Proteins; D006133:Growth Substances