title: Induction of the c-fos proto-oncogene in the rat pineal gland during stress.
abstract: To investigate the effects of stressful stimuli on pineal gland activity, male Wistar albino rats (200-250 g, 2-4 per group) were submitted to 30 min of forced immobilization or to unilateral vibrissotomy 30 min before sacrifice. In situ hybridization was performed with a 35S-labelled 50-base oligonucleotide probe complementary to nucleotides 270-319 of rat c-fos on sections containing the pineal gland. Autoradiograms were quantified using a JAVA microdensitometer. Stressful stimuli induced a significant increase in the expression of c-fos mRNA in the pineal gland (restraint = 144.3 +/- 14.4 cpm/mm2; hemivibrissotomy = 206.7 +/- 29.5 cpm/mm2) as compared to no restraint animals (30.6 +/- 5.1 cpm/mm2), animals displaying tonic-clonic seizures after an ip (64 mg/kg) injection of pentylenetetrazole (34.0 +/- 4.7 cpm/mm2), or competition (70.6 +/- 11.4 cpm/mm2) and RNAase-treated (52.7 +/- 9.1 cpm/mm2) controls. These results raise the possibility that stressful stimuli may interfere with pineal gland function.
authors.affiliation: Departamentos de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
authors.forename: E A
authors.initials: EA
authors.lastname: Del-Bel
authors.forename: R
authors.initials: R
authors.lastname: Titze-de-Almeida
authors.forename: H
authors.initials: H
authors.lastname: Shida
authors.forename: N
authors.initials: N
authors.lastname: Garcia-Cairasco
authors.forename: F M
authors.initials: FM
authors.lastname: Corrêa
authors.forename: F S
authors.initials: FS
authors.lastname: Guimarães
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000502:physiology; D016762:Genes, fos / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D010870:Pineal Gland / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000235:genetics / Q000378:metabolism*
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger