title: Immunohistochemical detection of tissue-infiltrating lymphocytes in mild urothelial dysplasia.
abstract: The presence and distribution of immunocompetent cells in normal and dysplastic urothelium of the bladder were investigated in 3 patients. Using an indirect immunoperoxidase staining procedure, monoclonal antibodies reacting with B (Leu12+) and T (Leu4+) cells, cells of the suppressor/cytotoxic (Leu2a+), helper/inducer (Leu3a+) and natural killer (Leu7+) phenotypes, monocyte-macrophages (LeuM3+), and cells expressing interleukin-2 receptor and HLA-DR antigen (HLA-DR+) were tested in all specimens. The results confirmed the presence of a well ordered and compartmentalised distribution of immunocompetent cells in the normal mucosa far from dysplasia. There was an increased number of lymphocytes in the hyperplastic/dysplastic urothelium with associated Von Brunn's nests. Leu2a+ cells, HLA-DR expressing cells and monocyte-macrophages were present inside the epithelium, particularly within dysplasia and von Brunn's nests, but the Leu3a+/Leu2a+ ratio was not inverted (0.48 + 0.1 to 0.74 + 0.2). These findings reveal that in mild urothelial dysplasia with von Brunn's nests there is a spatial modification of the immunological barrier associated with the mucosa.
authors.affiliation: Department of Urology, University of Cagliari, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Migliari
authors.forename: M I
authors.initials: MI
authors.lastname: el Demiry
authors.forename: G
authors.initials: G
authors.lastname: Muscas
authors.forename: M
authors.initials: M
authors.lastname: Melis
authors.forename: E
authors.initials: E
authors.lastname: Usai
mesh_terms: D000328:Adult; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D006965:Hyperplasia / Q000276:immunology; D007124:Immunoenzyme Techniques; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D011230:Precancerous Conditions / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D001743:Urinary Bladder / Q000276:immunology* / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
chemical_list: D006684:HLA-DR Antigens