A housekeeping type promoter, located in the 3' region of the Duchenne muscular dystrophy gene, controls the expression of Dp71, a major product of the gene.
The 70.8 kDa protein product of the distal part of the giant Duchenne muscular dystrophy (DMD) gene, Dp71, is expressed in many cell types and tissues. Anchored PCR, primer extension and functional analysis of transfected constructs were used to determine the 5' end of the mRNA and characterize the promoter of this major DMD gene product. The 5' untranslated region (5'UTR) of Dp71 is transcribed from a single exon; the promoter does not contain a TATA box, and has a very high GC content and several potential Sp1 binding sites. It is located more than 2000 kb 3' to the muscle and brain type dystrophin promoters and only 150 kb from the 3' end of the gene, suggesting that in most DMD patients the expression of Dp71 is unaffected.
Lederfein
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Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Yaffe
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Nudel
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D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D006528:Carcinoma, Hepatocellular; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016189:Dystrophin / Q000031:analogs & derivatives* / Q000235:genetics; D005091:Exons; D006801:Humans; D008113:Liver Neoplasms; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000235:genetics*; D009447:Neuroblastoma; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D016189:Dystrophin; D012333:RNA, Messenger; C085339:apo-dystrophin 1; D015500:Chloramphenicol O-Acetyltransferase