title: Quantitative measurement of hemosiderin deposition in tissue sections of the liver by image analysis.
abstract: Hemosiderin has been quantitated in tissue sections by image analysis employing Perls' Berlin blue reaction. A linear correlation was observed between the mean optical density of the tissue section measured with a commercial image analyzer and the iron concentration measured with an atomic absorption spectrophotometer. The correlation coefficient of .945 (P < .001) indicated that a linear correlation existed between these measurements. Thus, the hemosiderin volume in tissue sections can be measured quantitatively by image analysis.
authors.affiliation: Department of Pathology, School of Medicine, Iwate Medical University, Morioka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: T
authors.initials: T
authors.lastname: Kasai
authors.forename: R
authors.initials: R
authors.lastname: Satodate
mesh_terms: D006485:Hemosiderin / Q000032:analysis*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D013054:Spectrophotometry, Atomic
chemical_list: D006485:Hemosiderin