Co-expression of HSP72 and c-fos in rat brain following kainic acid treatment.
The relationship between heat shock protein 72 (HSP72) and c-fos gene expression following systemic administration of kainic acid was investigated by combining immunocytochemistry for HSP72 with in situ hybridization for c-fos. Increased HSP72 expression was detected in adult rat hippocampus 4 h after seizure-onset. Transient co-expression of c-fos and HSP72 occurred in neurons that are resistant to kainic acid, whereas prolonged co-expression was observed in vulnerable neurons. The spatial distribution and developmental time course of kainic acid-induced HSP72 expression were similar to those of kainic acid-induced neurodegeneration. The results demonstrate a relationship between c-fos and HSP72 gene expression and suggest that prolonged co-expression of these genes plays a role in kainic acid-induced neuronal death.
Schreiber
S S
SS
Department of Neurology, University of Southern California, School of Medicine, Los Angeles 90033.
Najm
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Tocco
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Baudry
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M
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D009410:Nerve Degeneration / Q000187:drug effects; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D006360:Heat-Shock Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D007608:Kainic Acid