The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein.
Expression of beta-galactosidase encoded by the nrg-29::Tn917-lacZ insertion increases 4,000-fold during nitrogen-limited growth (M.R. Atkinson and S. H. Fisher, J. Bacteriol. 173:23-27, 1991). The chromosomal DNA adjacent to the nrg-29::Tn917-lacZ insertion was cloned and sequenced. Analysis of the resulting nucleotide sequence revealed that the Tn917-lacZ transposon was inserted into the first gene of a dicistronic operon, nrgAB. The nrgA gene encodes a 43-kDa hydrophobic protein that is likely to be an integral membrane protein. The nrgB gene encodes a 13-kDa protein that has significant sequence similarity with the Escherichia coli glnB-encoded PII protein. Primer extension analysis revealed that the nrgAB operon is transcribed from a single promoter. The nucleotide sequence of this promoter has significant similarity with the -10 region, but not the -35 region, of the consensus sequence for Bacillus subtilis sigma A-dependent promoters.
Wray
L V
LV
Department of Microbiology, Boston University School of Medicine, Massachusetts 02118.
Atkinson
M R
MR
Fisher
S H
SH
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon / Q000235:genetics*; D051922:PII Nitrogen Regulatory Proteins; D010641:Phenotype; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
D000596:Amino Acids; D001426:Bacterial Proteins; D008565:Membrane Proteins; C085245:NrgA protein, Bacillus subtilis; C085246:NrgB protein, Bacillus subtilis; D051922:PII Nitrogen Regulatory Proteins; D000644:Quaternary Ammonium Compounds; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C404143:PIID regulatory protein, Bacteria; D001616:beta-Galactosidase