Cloning of the polyubiquitin cDNA from the marine sponge Geodia cydonium and its preferential expression during reaggregation of cells.
Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and characterized from the marine sponge Geodia cydonium. The open reading frame contains six ubiquitin moieties, which are lined up head to tail without spacers. A comparison of the predicted amino acid sequence of the six sponge ubiquitin-coding units with those from other organisms revealed a high degree of homology (> 93%). The ubiquitin gene is expressed to almost the same extent in the two main compartments of the sponge, the cortex and the medulla. However, only in the cortex are detectable amounts of the ubiquitin protein synthesized. The ubiquitin protein isolated from the sponge organism was found to initiate protein degradation in the heterologous reticulocyte system in the same manner as bovine ubiquitin. In vitro studies with dissociated sponge cells revealed that the homologous aggregation factor causes (i) a strong increase in the steady-state level of mRNA coding for ubiquitin and (ii) a drastic increase in ubiquitin protein synthesis, while the homologous lectin failed to display that effect in isolated cells. These data suggest that ubiquitin may play a role in sponge morphogenesis.
Pfeifer
K
K
Institut für Physiologische Chemie, Universität, Mainz, Germany.
Frank
W
W
Schröder
H C
HC
Gamulin
V
V
Rinkevich
B
B
Batel
R
R
Müller
I M
IM
Müller
W E
WE
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001704:Biopolymers / Q000096:biosynthesis / Q000235:genetics*; D015815:Cell Adhesion Molecules; D002449:Cell Aggregation / Q000187:drug effects; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D025821:Polyubiquitin; D011161:Porifera / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014452:Ubiquitins / Q000096:biosynthesis / Q000235:genetics*
D001704:Biopolymers; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D014452:Ubiquitins; C013065:cell aggregation factors; D025821:Polyubiquitin; D000255:Adenosine Triphosphate