Application of the time-resolved immunofluorometric assay to the study of C3 complement component glycation in vitro and in vivo.
A time-resolved immunofluorometric assay (TR-IFMA) was used for the measurement of glycated C3. The very high sensitivity of this technique allowed the direct measurement of glycated and non-glycated proteins (especially C3) in chromatography eluates. C3 glycation in vitro after incubation with 20 mmol/l glucose was always less than 3.5% by day 5. As determined with the TR-IFMA, the means +/- standard deviations of glycated C3 were 0.20% +/- 0.04 for non-diabetic subjects and 0.88% +/- 0.06 for insulin-dependent diabetic patients. The low percentages of glycated C3 in both our in vitro and in vivo studies show that this protein is subject to only moderate rates of glycation.
Gaillard
O
O
Laboratoire d'Immunochimie, Hôpital de la Salpêtrière, Paris, France.
Meillet
D
D
Bordas-Fonfrède
M
M
Khalil
L
L
Galli
J
J
Delattre
J
J
D000293:Adolescent; D000328:Adult; D000936:Antigen-Antibody Complex / Q000378:metabolism; D003176:Complement C3 / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D015200:Fluoroimmunoassay; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged
D000936:Antigen-Antibody Complex; D003176:Complement C3