A simple method for the determination of articaine and its metabolite articainic acid in dentistry: application to a comparison of articaine and lidocaine concentrations in alveolus blood.
This study was undertaken to develop a time- and cost-effective method for the detection of articaine and articainic acid in alveolus blood by high-performance liquid chromatography with a simple method of sample pretreatment. To overcome the problem of very rapid hydrolysis a method for controlling hydrolysis in vitro after blood sampling was developed. Blood samples were withdrawn from the alveolus of the upper molars 2-14 min after submucous injection of articaine (2.0 ml 4%) or identical injection of lidocaine (2.0 ml 2%). The higher blood levels found for articaine correspond to the higher concentration of the drug in the injection solution. A relationship between the serum concentration of articaine and lidocaine, respectively, and the time between injection and blood sampling could be established.
Oertel
R
R
Institute of Clinical Pharmacology, Medical Academy C.G. Carus, Dresden, FRG.
Richter
K
K
Weile
K
K
Gramatté
T
T
Berndt
A
A
Feller
K
K
D000539:Alveolar Process / Q000098:blood supply*; D002355:Carticaine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D008012:Lidocaine / Q000008:administration & dosage / Q000097:blood; D015203:Reproducibility of Results; D014081:Tooth Extraction
C055853:2-carboxyarticaine; D008012:Lidocaine; D002355:Carticaine