title: Suppression of yeast geranylgeranyl transferase I defect by alternative prenylation of two target GTPases, Rho1p and Cdc42p.
abstract: Geranylgeranyl transferase I (GGTase I), which modifies proteins containing the sequence Cys-Ali-Ali-Leu (Ali: aliphatic) at their C-termini, is indispensable for growth in the budding yeast Saccharomyces cerevisiae. We report here that GGTase I is no longer essential when Rho1p and Cdc42p are simultaneously overproduced. The lethality of a GGTase I deletion is most efficiently suppressed by provision of both Rho1p and Cdc42p with altered C-terminal sequences (Cys-Ali-Ali-Met) corresponding to the C-termini of substrates of farnesyl transferase (FTase). Under these circumstances, the FTase, normally not essential for growth of yeast, becomes essential.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, California 94305-5120.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohya
authors.forename: H
authors.initials: H
authors.lastname: Qadota
authors.forename: Y
authors.initials: Y
authors.lastname: Anraku
authors.forename: J R
authors.initials: JR
authors.lastname: Pringle
authors.forename: D
authors.initials: D
authors.lastname: Botstein
mesh_terms: D019883:Alkyl and Aryl Transferases; D001483:Base Sequence; D003001:Cloning, Molecular; D051231:Farnesyltranstransferase; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D005838:Genotype; D051228:Geranyltranstransferase; D008969:Molecular Sequence Data; D017368:Protein Prenylation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D014166:Transferases / Q000378:metabolism*; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; D020741:rho GTP-Binding Proteins
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051228:Geranyltranstransferase; D051231:Farnesyltranstransferase; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C051302:RHO1 protein, S cerevisiae; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; D020741:rho GTP-Binding Proteins