A human amphotropic retrovirus receptor is a second member of the gibbon ape leukemia virus receptor family.
Retrovirus infection is initiated by binding of the viral envelope glycoprotein to a cell-surface receptor. The envelope proteins of type C retroviruses of mammals demonstrate similarities in structural organization and protein sequence. These similarities suggest the possibility that retroviruses from different interference groups might use related proteins as receptors, despite the absence of any relationship between retrovirus receptors isolated to date. To investigate this possibility, we have identified a human cDNA clone encoding a protein closely related to the receptor for gibbon ape leukemia virus and have found that it functions as the receptor for the amphotropic group of murine retroviruses. Expression of this protein (GLVR-2) is likely to be a requirement for infection of human cells by amphotropic retroviral vectors for purposes of gene therapy.
van Zeijl
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M
Molecular Biology Research Section, American Cyanamid Company, Pearl River, NY 10965.
Johann
S V
SV
Closs
E
E
Cunningham
J
J
Eddy
R
R
Shows
T B
TB
O'Hara
B
B
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D017989:Leukemia Virus, Gibbon Ape / Q000378:metabolism*; D009052:Leukemia Virus, Murine / Q000378:metabolism; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000235:genetics*; D012190:Retroviridae / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D050611:Sodium-Phosphate Cotransporter Proteins, Type III
D002352:Carrier Proteins; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011991:Receptors, Virus; C493589:SLC20A2 protein, human; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; C070506:ecotropic murine leukemia virus receptor; C067981:leukemia virus receptor, gibbon ape