Continuous-flow solid (gel)-phase peptide synthesis using unsupported ultrahigh-load polymers: Fmoc/t-butyl strategy.
The technique of continuous-flow solid-phase peptide synthesis using unsupported polymers has been extended to cover the use of N alpha-Fmoc protected amino acids. This approach to peptide synthesis uses (but is not limited to) a phenolic bead-form polymer at 5 mmol g-1 loading. The success of the technique is based upon "layered displacement" to efficiently remove spent reagents and washing solutions from within the flow reactor under low pressure conditions. This system has been successfully employed in synthesizing the test peptides, [Leu5]-enkephalin, neurotensin and the notoriously difficult decapeptide sequence (65-74) of the acyl carrier protein.
Johnson
T
T
School of Applied Sciences, Wolverhampton University, Wolverhampton, UK.
Coffey
A F
AF
D000213:Acyl Carrier Protein / Q000737:chemistry; D000595:Amino Acid Sequence; D000596:Amino Acids; D002851:Chromatography, High Pressure Liquid; D004121:Dimethyl Sulfoxide; D004743:Enkephalin, Leucine / Q000138:chemical synthesis; D005449:Fluorenes; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008752:Methylene Chloride; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009496:Neurotensin / Q000138:chemical synthesis; D009842:Oligopeptides / Q000138:chemical synthesis; D010455:Peptides / Q000138:chemical synthesis*; D011108:Polymers; D012997:Solvents
D000213:Acyl Carrier Protein; D000596:Amino Acids; D005449:Fluorenes; D007202:Indicators and Reagents; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D009842:Oligopeptides; D010455:Peptides; D011108:Polymers; D012997:Solvents; D009496:Neurotensin; D004743:Enkephalin, Leucine; D008752:Methylene Chloride; D004121:Dimethyl Sulfoxide