Carboxyl methylation of protein phosphatase 2A from Xenopus eggs is stimulated by cAMP and inhibited by okadaic acid.
Protein phosphatase 2A is the principal carboxyl methylated protein in cytoplasmic extracts derived from Xenopus eggs arrested in interphase. Levels of methylation approach stoichiometric values after 2 hr of incubation at 22 degrees C. Rates of PP2A methylation increase by over 50% upon addition of cAMP. Methylation is inhibited by okadaic acid at concentrations that inhibit PP2A phosphatase activity.
Floer
M
M
Department of Molecular Biology, Princeton University, NJ 08544.
Stock
J
J
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D000242:Cyclic AMP / Q000494:pharmacology*; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004988:Ethers, Cyclic / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008745:Methylation; D019319:Okadaic Acid; D010063:Ovum / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D054648:Protein Phosphatase 2; D012436:S-Adenosylmethionine / Q000378:metabolism*; D015290:Second Messenger Systems; D013379:Substrate Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014982:Xenopus laevis
D002147:Calmodulin; D002364:Caseins; D004988:Ethers, Cyclic; D019319:Okadaic Acid; D012436:S-Adenosylmethionine; D000242:Cyclic AMP; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium