Reactive oxygen species of neutrophils from patients with monosomy 7 in the bone marrow: contradictory chemiluminescence activity by whole blood or by purified cells.
Monosomy 7, a deletion of the long arm of chromosome 7, was shown in the neutrophils of peripheral blood in 5 of 6 patients with a myelodysplastic syndrome or leukemia who had monosomy 7 in their bone marrow cells. In a chemiluminescence assay the production of reactive oxygen species by neutrophils from patients was increased in whole blood and decreased in purified cells, which suggests that neutrophils with monosomy 7 tolerate poorly the cell purification procedures used. The in vitro migration of purified neutrophils obtained from patients with monosomy 7 was impaired. It is known that patients with monosomy 7 have an increased susceptibility to infections. It is possible that neutrophils with monosomy 7 are too easily triggered to a full-scale respiratory burst and thereby the cells exhaust their ability to eliminate invading microbes efficiently.
Ristola
M
M
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Kere
J
J
Ruutu
T
T
Repo
H
H
D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002634:Chemotaxis, Leukocyte; D002869:Chromosome Aberrations / Q000503:physiopathology*; D025063:Chromosome Disorders; D002897:Chromosomes, Human, Pair 7; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009006:Monosomy; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D017382:Reactive Oxygen Species
D017382:Reactive Oxygen Species