title: T-cell receptor V-gene usage in synovial fluid lymphocytes of patients with chronic arthritis.
abstract: In this study we analyzed the usage frequencies of the TCR V-gene segments by alpha beta+ T cells present in synovial fluid of 17 patients with chronic arthritis, including rheumatoid arthritis. The results of this study, obtained from semiquantitative PCR analyses, showed that in all patients most of the TCR V alpha- and V beta-gene segments could be detected both in fresh PBMCs and in fresh SFMCs. The relative frequencies of use of these V-region genes were variable between the different patients. Although there was some skewing of increased usage frequencies of particular TCR V alpha and V beta genes among SFMC-derived TCRs when compared with PBMCs, we could not correlate such increased TCR V-gene usage with the inflammation in the joints as a disease-specific marker.
authors.affiliation: Department of Immunohematology and Bloodbank, Leiden University Hospital, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Struyk
authors.forename: J T
authors.initials: JT
authors.lastname: Kurnick
authors.forename: G E
authors.initials: GE
authors.lastname: Hawes
authors.forename: J M
authors.initials: JM
authors.lastname: van Laar
authors.forename: R
authors.initials: R
authors.lastname: Schipper
authors.forename: J R
authors.initials: JR
authors.lastname: Oksenberg
authors.forename: L
authors.initials: L
authors.lastname: Steinman
authors.forename: R R
authors.initials: RR
authors.lastname: de Vries
authors.forename: F C
authors.initials: FC
authors.lastname: Breedveld
authors.forename: P
authors.initials: P
authors.lastname: van den Elsen
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001483:Base Sequence; D015139:Blotting, Southern; D002908:Chronic Disease; D004247:DNA / Q000032:analysis; D005260:Female; D015870:Gene Expression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013582:Synovial Fluid / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D009841:Oligonucleotides; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA