Enterocytic differentiation of the human Caco-2 cell line is correlated with down-regulation of fibronectin and laminin.
Human intestinal Caco-2 cells were used to examine the expression of fibronectin (FN) and laminin (LN) during enterocytic differentiation. Combination of immunoprecipitation, Western and Northern blotting revealed that Caco-2 cells expressed a classical FN and a variant form of LN: besides B1 and B2 chains, LN contained a 350-kDa heavy chain instead of the 400-kDa A chain. Throughout Caco-2 cell differentiation, FN and LN synthesis decreased at both mRNA and protein levels. These data indicate that enterocytic differentiation involves both transcriptional and/or post-transcriptional down-regulation of FN and LN gene expression.
Levy
P
P
Laboratoire de Biochimie-Biologie cellulaire, INSERM-U 181, Faculté Médecine Saint Antoine, Paris, France.
Loreal
O
O
Munier
A
A
Yamada
Y
Y
Picard
J
J
Cherqui
G
G
Clement
B
B
Capeau
J
J
D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D003470:Culture Media / Q000378:metabolism; D015536:Down-Regulation; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D007797:Laminin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D010452:Peptide Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
D003470:Culture Media; D005353:Fibronectins; D007797:Laminin; D012333:RNA, Messenger