Glucocorticoid receptor binding in proliferating and differentiating cells: modulation by a tumour promoting agent.
The effects of the tumour-promoting agent, phorbol 12-myristate 13-acetate, on dexamethasone binding and protein kinase C in proliferating and differentiating murine erythroleukemic cells were investigated. Concentration dependent changes in steroid binding were observed in proliferating and differentiating cells treated with phorbol 12-myristate 13-acetate for 2h or 24h. Ligand binding in differentiating cells was consistently lower than in proliferating cells. Reduced expression of protein kinase C was observed in differentiating cells as compared with proliferating cells; the amount of enzyme protein was reduced on treatment of cells with phorbol ester.
Lavis
G R
GR
Department of Medical Biochemistry, University of the Witwatersrand Medical School, Parktown, Johannesburg, South Africa.
Hammond
K D
KD
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D003907:Dexamethasone / Q000378:metabolism; D051379:Mice; D011493:Protein Kinase C / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate