title: Discrimination of epidemic and sporadic isolates of Arcobacter butzleri by polymerase chain reaction-mediated DNA fingerprinting.
abstract: DNA polymorphisms of Arcobacter butzleri outbreak-related strains and Arcobacter reference strains were determined by use of the polymerase chain reaction with primers aimed at repetitive sequences. The epidemiological relationship among 14 outbreak-related strains was substantiated, as they showed virtually no genomic variations. Their DNA amplification patterns were, however, clearly different from those of all Arcobacter reference strains studied; each reference strain was characterized by a unique DNA fingerprint.
authors.affiliation: Department of Microbiology, University Hospital, Antwerp, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Vandamme
authors.forename: B A
authors.initials: BA
authors.lastname: Giesendorf
authors.forename: A
authors.initials: A
authors.lastname: van Belkum
authors.forename: D
authors.initials: D
authors.lastname: Pierard
authors.forename: S
authors.initials: S
authors.lastname: Lauwers
authors.forename: K
authors.initials: K
authors.lastname: Kersters
authors.forename: J P
authors.initials: JP
authors.lastname: Butzler
authors.forename: H
authors.initials: H
authors.lastname: Goossens
authors.forename: W G
authors.initials: WG
authors.lastname: Quint
mesh_terms: D000818:Animals; D001483:Base Sequence; D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial