hobo enhancer trapping mutagenesis in Drosophila reveals an insertion specificity different from P elements.
P element enhancer trapping has become an indispensable tool in the analysis of the Drosophila melanogaster genome. However, there is great variation in the mutability of loci by these elements such that some loci are relatively refractory to insertion. We have developed the hobo transposable element for use in enhancer trapping and we describe the results of a hobo enhancer trap screen. In addition, we present evidence that a hobo enhancer trap element has a pattern of insertion into the genome that is different from the distribution of P elements in the available database. Hence, hobo insertion may facilitate access to genes resistant to P element insertion.
Smith
D
D
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
Wohlgemuth
J
J
Calvi
B R
BR
Franklin
I
I
Gelbart
W M
WM
D000818:Animals; D030801:Animals, Genetically Modified; D002875:Chromosomes; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005260:Female; D005804:Genes, Lethal; D006720:Homozygote; D008297:Male; D016296:Mutagenesis
D004251:DNA Transposable Elements