Neuropeptide F-immunoreactivity in the tetrathyridium of Mesocestoides corti (Cestoda: Cyclophyllidea).
The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage of Mesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30-39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified.
Hrckova
G
G
Parasitological Institute, Slovak Academy of Sciences, Kosice.
Halton
D W
DW
Maule
A G
AG
Brennan
G P
GP
Shaw
C
C
Johnston
C F
CF
D000818:Animals; D015801:Helminth Proteins / Q000276:immunology / Q000302:isolation & purification*; D007150:Immunohistochemistry; D008645:Mesocestoides / Q000737:chemistry* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D009420:Nervous System / Q000737:chemistry*; D009479:Neuropeptides / Q000276:immunology / Q000302:isolation & purification*; D014018:Tissue Distribution
D015801:Helminth Proteins; D009479:Neuropeptides; C076101:neuropeptide F