The covalent linkage of secretory component to IgA. Structure of sIgA.
Immunoglobulin A which is secreted into external fluids is synthesized in plasma cells as an (IgA)2-J-chain complex. This complex docks on to the polyimmunoglobulin receptor which is located at the basolateral surface of epithelial cells. After docking the (IgA)2-J-receptor complex is internalized and processed. The polyimmunoglobulin receptor loses its C-terminal tail and thus becomes the secretory component. This secretory component is then covalently linked to the (IgA)2-J-chain complex by a disulfide bond, and protects the so formed sIgA from denaturation and proteolysis in external fluids. In order to establish this disulfide bond between IgA and the secretory component, sIgA, purified from human colostrum, was subjected to several enzymatic and chemical fragmentation reactions. One of the resulting polypeptides allowed us to characterize the covalent linkage of the secretory component to IgA in sIgA. IgA was found to be covalently linked to the secretory piece by a single disulfide bond between Cys 311 of one alpha-chain and Cys 467 of the secretory component. Cys 501 of the secretory component and Cys 311 of the other alpha-chain are blocked by cysteines. With this last paper of a series the structure of an entire sIgA molecule has been elucidated.
Fallgreen-Gebauer
E
E
Max-Planck-Institut für Experimentelle Medizin, Göttingen.
Gebauer
W
W
Bastian
A
A
Kratzin
H D
HD
Eiffert
H
H
Zimmermann
B
B
Karas
M
M
Hilschmann
N
N
D000595:Amino Acid Sequence; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D003126:Colostrum / Q000276:immunology; D003488:Cyanogen Bromide; D004220:Disulfides; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000378:metabolism; D011487:Protein Conformation; D012635:Secretory Component / Q000737:chemistry*; D013820:Thermolysin / Q000378:metabolism
D004220:Disulfides; D007071:Immunoglobulin A, Secretory; D012635:Secretory Component; D010434:Pepsin A; D013820:Thermolysin; D003488:Cyanogen Bromide