Automated process monitoring of monoclonal antibody production.
Antifibronectin, monoclonal antibody was monitored through 52 h of production. Samples were automatically drawn from a bioreactor into the injection valve of an HPLC system without prior sample preparation. The hybridoma cell line was nonadherent, so whole cells were injected directly onto the perfusable protein A affinity column. There was only a modest column back pressure (ca. 1700 psi at a linear flow rate of 1.5 cm/s) after over 75 injections over the 52-h experiment. These experiments demonstrate the utility of high-speed chromatography for rapid process monitoring.
Paliwal
S K
SK
Department of Chemistry, Purdue University, Lafayette, Indiana 47907.
Nadler
T K
TK
Wang
D I
DI
Regnier
F E
FE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D005353:Fibronectins / Q000276:immunology*; D006825:Hybridomas; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000096:biosynthesis*; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007074:Immunoglobulin G