Inhibition of cell cycle oscillation of DNA replication by a selective inhibitor of the cdc2 kinase family, butyrolactone I, in Xenopus egg extracts.
A selective inhibitor of the cdc2 kinase family, butyrolactone I, was found to inhibit the DNA replication activity of Xenopus egg extracts. When metaphase-arrested Xenopus egg extracts pretreated with butyrolactone I were released into interphase by the addition of CaCl2 at low concentrations of sperm nuclei, butyrolactone I (10 microM) prevented the oscillation of DNA replication activity to a great extent and lengthened the cell cycle. In contrast, in activated extracts which had already entered interphase, prepared from eggs activated by the calcium ionophore A23187, butyrolactone I (10 microM) had little effect on the first peak of the oscillation but reduced to less than half the second peak. These inhibitory effects of butyrolactone I on the initiation of DNA replication and mitosis in Xenopus egg extracts are probably a consequence of its previously reported inhibition of the cyclin-cdc2 kinase family.
Someya
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A
Applied Enzymology, Research Laboratory, Tsukuba Research Institute, Banyu Pharmaceutical Co., Ltd., Japan.
Tanaka
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N
Okuyama
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A
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D016203:CDC2 Protein Kinase / Q000037:antagonists & inhibitors*; D002453:Cell Cycle / Q000502:physiology*; D002474:Cell-Free System; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D010063:Ovum; D010507:Periodicity; D014981:Xenopus
C082868:butyrolactone I; D016203:CDC2 Protein Kinase; D015107:4-Butyrolactone