[Alteration of lipid peroxidation and the activity of peroxide metabolism enzymes in the liver, kidney and lung following the administration of paraquat in mice].
The sequential changes of the activity of peroxide metabolism enzymes and thiobarbituric acid reaction substance (TBARS) as an indicator of lipid peroxide concentration were observed to analyze the mechanisms of tissue injury due to the administration of paraquat in DDY mice. Each six mice were decapitated at 3, 6, 9, 12, 15, 18, 21 or 24 hours following the intraperitoneal administration of paraquat 60 mg.kg-1, and the liver, kidney and lung were removed and homogenized to quantify lipid peroxide concentration and the activity of peroxide metabolism enzymes. In the liver and kidney, TBARS increased gradually and reached the maximum level at 9 hours after the administration of paraquat in the liver and at 12 hours in the kidney. Significant increases in superoxide dismutase (SOD), glutathione peroxidase and catalase (CAT) activities were observed at 15 or 18 hours after the injection of paraquat. In the lung, lipid peroxide concentration decreased significantly and the activities of SOD and CAT increased at 15 hours after injection of paraquat. TBARS in serum started to increase at 9 hours after the administration of paraquat and reached the maximum level at 15 hours. These findings indicate that the induction of peroxide metabolism enzymes followed the increase of TBARS which was induced by paraquat in the liver and kidney. However, the enzyme induction was produced by paraquat without the increase of TBARS in the lung. Active oxygen radicals play an important role in the toxic action of paraquat and might produce the induction of peroxide metabolism enzymes following the administration of paraquat.
Takenaka
T
T
Department of Anesthesiology, School of Medicine, Kitasato University, Sagamihara.
Goto
F
F
D000818:Animals; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D010269:Paraquat / Q000633:toxicity*; D010545:Peroxides / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
D010545:Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D010269:Paraquat