In vitro effects of ethanol on gonadotropin-binding sites in porcine granulosa cells.
In this study, we examined the in vitro effects of ethanol on [125I]human follicle-stimulating hormone (hFSH) and [125I]human luteinizing hormone (hLH) binding to porcine granulosa cells (GCs). The GC suspensions and [125I]hFSH or [125I]hLH were incubated in the presence or absence of 1-50% (vol/vol) ethanol until steady state binding was attained for 2 h at 37 degrees C. The presence of 1-4% ethanol significantly enhanced the specific binding of hFSH to porcine GCs, with a maximal effect at 4% ethanol, however, had no effect on hLH binding. Higher concentration of ethanol (8-50%) significantly decreased both specific hFSH and hLH binding to porcine GCs. Scatchard analyses revealed that ethanol increased the number of FSH-binding sites without changing the affinity for FSH. The data suggest that porcine GCs contain a population of masked FSH-binding sites exposed by in vitro treatment with ethanol. Furthermore, the membrane fluidity may play an important role in the masking/unmasking mechanism of FSH-binding sites within the porcine GCs.
Nishimori
K
K
Department of Obstetrics and Gynecology, Wakayama Medical College, Japan.
Yamoto
M
M
Nakano
R
R
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007986:Luteinizing Hormone / Q000378:metabolism*; D011869:Radioligand Assay; D013552:Swine
D007457:Iodine Radioisotopes; D000431:Ethanol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone