title: Evidence for the presence of a high-affinity laminin receptor-like molecule on the surface of Candida albicans yeast cells.
abstract: Two polypeptides of 37 and 67 kDa that bind laminin were detected in cell wall extracts of Candida albicans blastoconidia. The 37-kDa species, found only in yeast cell wall extracts, cross-reacted with a rabbit polyclonal antibody (PAb 4160) directed towards the carboxyl-terminal laminin-binding domain present in the human 67-kDa high-affinity laminin receptor (67LR) and its 37-kDa precursor (37LRP), whereas another antibody (PAb 4056), directed against internal domains of 67LR and 37LRP, recognized a 37-kDa species in wall extracts from both blastoconidia and germinated blastoconidia. Indirect immunofluorescence with PAb 4160 showed a patchy binding pattern only on yeast cells that represented about 10% of the entire blastoconidia population.
authors.affiliation: Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79430.
authors.forename: J L
authors.initials: JL
authors.lastname: López-Ribot
authors.forename: M
authors.initials: M
authors.lastname: Casanova
authors.forename: C
authors.initials: C
authors.lastname: Monteagudo
authors.forename: P
authors.initials: P
authors.lastname: Sepúlveda
authors.forename: J P
authors.initials: JP
authors.lastname: Martínez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000908:Antibodies, Fungal; D001665:Binding Sites; D002176:Candida albicans / Q000276:immunology / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D002473:Cell Wall / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D017474:Receptors, Laminin / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D000908:Antibodies, Fungal; D009842:Oligopeptides; D017474:Receptors, Laminin; C047981:arginyl-glycyl-aspartic acid