In situ growth and activity and modes of penetration of Escherichia coli in unconsolidated porous materials.
Statistically reliable data on the in situ rates of growth, substrate consumption, and product formation are required to test the validity of the mathematical models developed for microbially enhanced oil recovery and in situ bioremediation processes. A simple, replicable porous-core system that could be aseptically divided into sections at various times was developed to follow the kinetics of microbial growth and metabolism in situ. This core system was used to study the kinetics of growth and the mode of penetration of strains of Escherichia coli through anaerobic, nutrient-saturated, fine Ottawa sand (permeability of 7.0 microns2 and porosity of 37%) under static conditions. The in situ rate of growth of a wild-type, motile, chemotactic strain, RW262, was two times slower inside cores than it was in liquid cultures. The mode of metabolism of galactose by strain RW262 was not altered inside cores, as acetate was the only product detected either inside the cores or in liquid cultures. Without applied advective force, strain RW262 grew exponentially and moved through cores at a rate of about 0.1 m/day. The cell population moved through cores in a band-like fashion, as the front of the moving cells consisted of high cell concentrations (greater than 10(5) cells per ml). Until the breakthrough of the cells occurred, galactose consumption and acetate production were observed only in the proximal sections of the core, showing that the cell propagation preceded the complete depletion of the substrate or the accumulation of large amounts of products.(ABSTRACT TRUNCATED AT 250 WORDS)
Sharma
P K
PK
Department of Botany and Microbiology, University of Oklahoma, Norman 73019-0245.
McInerney
M J
MJ
Knapp
R M
RM
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D002465:Cell Movement / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology; D004926:Escherichia coli / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology*; D005690:Galactose / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D012822:Silicon Dioxide; D013045:Species Specificity; D014871:Water Microbiology
D000085:Acetates; D012822:Silicon Dioxide; D019342:Acetic Acid; D005690:Galactose