Rapid determination of glutamine in biological samples by high-performance liquid chromatography.
A high-performance liquid chromatographic method for the determination of glutamine in biological samples is presented. Glutamine was derivatized with ortho-phthalaldehyde reagent containing 3-mercaptopropionic acid and separated by reversed phase chromatography on a C18 column containing 3-microns particles. No interference from other amino acids was observed. The assay was linear over a range from 1 to 2,000 mumol/l. Analytical recovery of plasma samples spiked with glutamine was 98.6 +/- 3.8%. Within- and between-batch imprecision were 1.5% and 2.2%, respectively. The derivatization step was fully automated. Total analysis time, including derivatization and chromatography, amounted to 6 min. The method can be used for the determination of glutamine in plasma, urine, cerebrospinal fluid and tissue homogenates.
Teerlink
T
T
Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands.
Hennekes
M W
MW
van Leeuwen
P A
PA
Houdijk
A
A
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D005973:Glutamine / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D006801:Humans; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds; D009764:o-Phthalaldehyde
D013438:Sulfhydryl Compounds; D005973:Glutamine; D009764:o-Phthalaldehyde; C023884:thiolactic acid