Detection of Cryptosporidium parvum using a specific polymerase chain reaction.
The design and use of polymerase chain reaction primers and probes as reagents for the detection of Cryptosporidium parvum are described. Sensitive and specific amplification of a 329 base pair product was demonstrated by ethidium bromide staining and hybridisation of radiolabelled probes. These reagents have the potential for application to diagnostic samples, environmental monitoring and epidemiological surveys.
Webster
K A
KA
Central Veterinary Laboratory, New Haw, Weybridge, UK.
Pow
J D
JD
Giles
M
M
Catchpole
J
J
Woodward
M J
MJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D016785:Cryptosporidium parvum / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis* / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013045:Species Specificity
D017931:DNA Primers; D016054:DNA, Protozoan; D015345:Oligonucleotide Probes