Utilization of xylooligosaccharides by selected ruminal bacteria.
The ability of ruminal bacteria to utilize xylooligosaccharides was examined. Xylooligosaccharides were prepared by partially hydrolyzing oat spelt xylan in phosphoric acid. This substrate solution was added (0.2%, wt/vol) to a complex medium containing yeast extract and Trypticase that was inoculated with individual species of ruminal bacteria, and growth and utilization were monitored over time. All of the xylanolytic bacteria examined were able to utilize this oligosaccharide mixture as a growth substrate. Butyrivibrio fibrisolvens, Eubacterium ruminantium, and Ruminococcus albus used xylooligosaccharides and whole, unhydrolyzed xylan to similar extents, while Prevotella ruminicola used twice as much xylooligosaccharides as xylan (76 versus 34%). Strains of Selenomonas ruminantium were the only nonxylanolytic species that were able to grow on xylooligosaccharides. The ability of individual S. ruminantium strains to utilize xylooligosaccharides was correlated with the presence of xylosidase and arabinosidases activities.
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Fermentation Biochemistry Research Unit, U.S. Department of Agriculture, Peoria, Illinois 61604.
D000818:Animals; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005051:Eubacterium / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006089:Gram-Negative Anaerobic Bacteria / Q000378:metabolism; D006095:Gram-Positive Cocci / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000378:metabolism
D009844:Oligosaccharides; D014990:Xylans; D006026:Glycoside Hydrolases; D014995:Xylosidases; C019713:alpha-N-arabinofuranosidase