Spontaneous dimerization of retroviral MoMuLV RNA.
The genome of the Moloney murine leukemia virus (MoMuLV) is composed of two identical RNA molecules joined at their 5' ends by the dimer linkage structure (DLS). Dimerization sequences are located within the PSI encapsidation domain. We present here an overview of the work we have performed on spontaneous dimerization of a MoMuLV RNA fragment encompassing the PSI domain in order to understand the mechanism by which retroviral RNA dimerization takes place. We present kinetical, thermodynamical and conformational evidence which leads to the conclusion that the PSI domain is a structurally independent domain and that conformational changes are triggered by the dimerization process. We conclude that at least one particular region (nucleotides 278-309) of the RNA is directly involved in the process while the conformation of some other regions is changed probably because of a long-range effect.
Paoletti
J
J
Unité de Biochimie, URA 147 CNRS and U140 INSERM, Institut Gustave-Roussy, Villejuif, France.
Mougel
M
M
Tounekti
N
N
Girard
P M
PM
Ehresmann
C
C
Ehresmann
B
B
D001483:Base Sequence; D001704:Biopolymers; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry*
D001704:Biopolymers; D012367:RNA, Viral