title: A calcium/calmodulin-binding serine/threonine protein kinase homologous to the mammalian type II calcium/calmodulin-dependent protein kinase is expressed in plant cells.
abstract: cDNA fragments corresponding to an apple (Malus domestica [L.] Borkh) calmodulin-binding polypeptide have been isolated and characterized. The protein encoded by this messenger contains a serine/threonine protein kinase catalytic domain followed by a calcium/calmodulin-binding regulatory domain, both exhibiting significant sequence similarities to the corresponding regions of the mammalian calcium/calmodulin-dependent protein kinase II subunits. These results confirm a potential regulatory role for calmodulin in phosphorylation-mediated signal transduction events.
authors.affiliation: Faculté des Sciences Agronomiques, Unité de Biologie Moléculaire et Physiologie Animale, Gembloux, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Watillon
authors.forename: R
authors.initials: R
authors.lastname: Kettmann
authors.forename: P
authors.initials: P
authors.lastname: Boxus
authors.forename: A
authors.initials: A
authors.lastname: Burny
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002384:Catalysis; D003001:Cloning, Molecular; D004247:DNA; D005638:Fruit / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D012333:RNA, Messenger; D004247:DNA; D017871:Calcium-Calmodulin-Dependent Protein Kinases