title: Neuronal interferon-gamma immunoreactive molecule: bioactivities and purification.
abstract: An interferon (IFN)-gamma immunoreactive molecule, localized to small neurons in peripheral sensory ganglia (N-IFN-gamma), has been detected with two mouse monoclonal antibodies (DB1 and DB16) directed against different epitopes of rat IFN-gamma. To define N-IFN-gamma with regard to its protein characteristics and bioactivities, DB1 and DB16 were used to purify N-IFN-gamma from rat trigeminal ganglia in a two-step sequential antibody-affinity procedure. Sodium dodecylsulfate polyacrylamide gel electrophoresis (PAGE) and silver staining of purified N-IFN-gamma displayed three bands with an approximate molecular mass of 66, 62 and 54 kDa. The N-IFN-gamma bioactivity was confined to the protein stained on gel when native material was run on PAGE. Biological effects of pure N-IFN-gamma were examined and compared with those of lymphocyte-derived recombinant IFN-gamma. N-IFN-gamma had antiviral effects in vitro and induced major histocompatibility complex class I and II antigens on macrophages and in cells in skeletal muscle cell cultures. N-IFN-gamma also stimulated myoblast proliferation and affected cholinergic receptor distribution on myotubes similar to recombinant IFN-gamma. Both molecules potently stimulated Trypanosoma brucei brucei growth. These data suggest that, although N-IFN-gamma is a protein distinct from lymphocyte-derived IFN-gamma, the two molecules have enough structural similarities to allow for antibody recognition of at least two epitopes, and action on similar target structures on both parasite and mammalian cells.
authors.affiliation: Department of Neurology, Huddinge University Hospital, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Olsson
authors.forename: S
authors.initials: S
authors.lastname: Kelic
authors.forename: C
authors.initials: C
authors.lastname: Edlund
authors.forename: M
authors.initials: M
authors.lastname: Bakhiet
authors.forename: B
authors.initials: B
authors.lastname: Höjeberg
authors.forename: P H
authors.initials: PH
authors.lastname: van der Meide
authors.forename: A
authors.initials: A
authors.lastname: Ljungdahl
authors.forename: K
authors.initials: K
authors.lastname: Kristensson
mesh_terms: D000818:Animals; D000998:Antiviral Agents; D001681:Biological Assay; D002478:Cells, Cultured; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000276:immunology / Q000302:isolation & purification*; D008264:Macrophages / Q000276:immunology; D009132:Muscles / Q000166:cytology; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011940:Receptor Aggregation / Q000187:drug effects; D011978:Receptors, Nicotinic / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000737:chemistry; D014345:Trypanosoma / Q000254:growth & development
chemical_list: D000998:Antiviral Agents; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D011978:Receptors, Nicotinic; D007371:Interferon-gamma