[Change of beta tubulin gene expression in the motoneurons of spinal cord during nerve regeneration].
After crushing the right sciatic nerve of rat, the contents of both tubulin and beta tubulin mRNA increased significantly in the ventral horns of spinal cord. Analysis of tubulin in bilateral ventral horns by SDS-PAGE showed that tubulin in the injured side increased by 17%-121% of the control side. The results of in situ hybridization indicated with the signals of hybridization with beta tubulin cDNA probes were 27%-70% higher in motoneurons of the ventral horns in the injury side than the control. Northern blotting analysis of tubulin mRNA in ventral horns also revealed an increase of tubulin gene expression.
He
B
B
Institute of Basic Medical Sciences, Beijing.
Fan
M
M
Liu
S H
SH
Gan
S D
SD
D000818:Animals; D015870:Gene Expression; D009046:Motor Neurons / Q000502:physiology*; D009416:Nerve Regeneration / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000502:physiology*; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics*
D014404:Tubulin