Proton nuclear magnetic resonance spectroscopy of urine and plasma in renal transplantation follow-up.
Urine and plasma samples from 39 patients who underwent renal transplantation were analyzed by proton nuclear magnetic resonance (NMR) spectroscopy. The most relevant resonances for evaluating renal function after transplantation were those arising from citrate, trimethylamine-N-oxide (TMAO), alanine, and lactate when compared to creatinine. A resonance at 3.7 ppm was related to cyclosporine toxicity when associated with elevated levels of TMAO. The respective variations in these metabolites in urine could contribute to prognosis and diagnosis of renal function impairment related to cyclosporine toxicity or overdosage, or to rejection. Thus, NMR spectroscopy should improve the follow-up and management of renal transplantation patients.
Le Moyec
L
L
Laboratoire de RMN et Exploration Fonctionnelle, Hôpital Saint-Louis, Paris, France.
Pruna
A
A
Eugène
M
M
Bedrossian
J
J
Idatte
J M
JM
Huneau
J F
JF
Tomé
D
D
D000409:Alanine / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001774:Blood Chemical Analysis; D002951:Citrates / Q000032:analysis; D019343:Citric Acid; D003404:Creatinine / Q000032:analysis; D016572:Cyclosporine / Q000009:adverse effects / Q000097:blood; D004123:Dimethylamines / Q000032:analysis; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis / Q000652:urine; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D007677:Kidney Function Tests / Q000379:methods*; D016030:Kidney Transplantation / Q000009:adverse effects / Q000502:physiology*; D007773:Lactates / Q000032:analysis; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy; D008744:Methylamines / Q000032:analysis; D014556:Urine / Q000737:chemistry*
D015415:Biomarkers; D002951:Citrates; D004123:Dimethylamines; D007773:Lactates; D008744:Methylamines; D019343:Citric Acid; D019344:Lactic Acid; D016572:Cyclosporine; C034516:dimethylamine; D003404:Creatinine; C005855:trimethyloxamine; D000409:Alanine