[Method of isolating carbamazepine from cadaveric material and its determination by fluorescent polarization immunoassay].
Optimal conditions for carbamazepine isolation from cadaveric liver have been determined. The authors recommend the material infusion with alcohol acidified with sulfuric acid to pH 1 three times for an hour. Conditions for measuring the isolated drug quantity have been developed. Fluorescent polarization immunoanalysis in the TDX system has been used for the first time. Carbamazepine detection limit for 50 g of the liver was found to be 2.5 x 10(-7) g.
Shabalin
A V
AV
Vergeĭchik
T Kh
TKh
D002102:Cadaver; D002220:Carbamazepine / Q000032:analysis / Q000302:isolation & purification*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry; D012995:Solubility
D002220:Carbamazepine
Metodika izolirovaniia karbamazepina iz trupnogo materiala i opredeleniia ego metodom fliuorestsentno-poliarizatsionnogo immunoanaliza.