[The determination of the genotype of laboratory reassortants of the influenza A virus by competitive dot hybridization].
The genotype of laboratory reassortants produced by co-cultivation of virulent A/USSR/2/85 (H3N2) virus and multi-passaged attenuation donor A/Leningrad/9/46 virus (H1N1) was determined by two different methods: a method of competitive dot hybridization (CDH) and polyacrylamide gel electrophoresis (PAGE) with virion RNAs urea. The results of the two methods were shown to be completely identical which confirmed the potential of using CDH method for genetic typing of influenza A virus reassortant strains.
Golubev
D B
DB
Zolotarev
F N
FN
Iukhnova
L G
LG
D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral / Q000235:genetics; D005838:Genotype; D015151:Immunoblotting; D009980:Influenza A virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics*
D012367:RNA, Viral
Opredelenie genotipa laboratornykh reassortantov virusa grippa A metodom konkurentnoĭ tochechnoĭ gibridizatsii.