title: Further studies of 31 temperature-sensitive mutants of mouse cytomegalovirus: thermal stability, replication and analysis of temperature-sensitive functions by temperature shift.
abstract: A study of 31 temperature-sensitive mutants of mouse cytomegalovirus has indicated that two mutants (tsm1, tsm31) may be defective in immediate-early/early functions, two (tsm2, tsm3) may be defective in early functions and six (tsm9, tsm18, tsm22, tsm23, tsm28, tsm30) may be defective in early/late functions while the remainder are late function-defective mutants as determined by temperature-shift experiments. Three mutants (tsm1, tsm2, tsm3) were more thermostable than wild-type virus while three (tsm16, tsm26, tsm28) were more thermolabile; the remainder were similar in their thermostability to wild-type virus.
authors.affiliation: School of Biological Sciences, Microbial Molecular Genetics and Cell Biology Research Group, University of Birmingham, Edgbaston, UK.
authors.forename: H M
authors.initials: HM
authors.lastname: Akel
authors.forename: A M
authors.initials: AM
authors.lastname: Furarah
authors.forename: C
authors.initials: C
authors.lastname: Sweet
mesh_terms: D018146:Muromegalovirus / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D013045:Species Specificity; D013696:Temperature; D014779:Virus Replication