Direct injection assay of drug enantiomers in serum on ovomucoid-bonded silica materials by liquid chromatography.
A high-performance liquid chromatographic (HPLC) method for the determination of drug enantiomers in serum was developed. The method involves direct injection of serum samples on to an ovomucoid-bonded column, which is prepared by bonding of ovomucoid proteins to an aminopropyl-silica gel by the N,N'-disuccinimidyl carbonate activation method and separation of drug enantiomers on the column using a mixture of phosphate buffer and an organic solvent. High recoveries of serum proteins were obtained using eluent pH values of 3, 4, 6 and 7 at phosphate buffer concentrations above 50 mM, whereas the recovery was ca. 70% at an eluent pH of 5. The recovery of each enantiomer of basic and acidic drugs from serum was almost 100%.
Haginaka
J
J
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Murashima
T
T
Fujima
H
H
Wada
H
H
D001798:Blood Proteins / Q000032:analysis; D002744:Chlorpheniramine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007660:Ketoprofen / Q000097:blood*; D010059:Ovomucin; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D001798:Blood Proteins; D010059:Ovomucin; D002744:Chlorpheniramine; D012822:Silicon Dioxide; D007660:Ketoprofen