title: Thrombospondin 3 is a developmentally regulated heparin binding protein.
abstract: The thrombospondins (TSPs) are a growing family of cell surface and extracellular matrix molecules composed of multiple repeating elements. Thrombospondin 3 is a recently described member that possesses the calcium binding Type 3 repeats, has 4 epidermal growth factor receptor-like Type 2 repeats, a complete absence of the complement-like Type 1 repeats, and a distinct N terminus that has no significant homology to the other TSPs. Metabolic labeling and immunoprecipitation analysis of cells transfected with a TSP3 expression vector revealed it to be an oligomeric heparin binding protein present in both the cell layer and medium. Finally, a combination of in situ hybridization and immunocytochemistry demonstrated TSP3 to be expressed in a temporal and spatial manner during murine embryogenesis, especially in the gut, cartilage, lung, and central nervous system.
authors.affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor 48019.
authors.forename: A N
authors.initials: AN
authors.lastname: Qabar
authors.forename: Z
authors.initials: Z
authors.lastname: Lin
authors.forename: F W
authors.initials: FW
authors.lastname: Wolf
authors.forename: K S
authors.initials: KS
authors.lastname: O'Shea
authors.forename: J
authors.initials: J
authors.lastname: Lawler
authors.forename: V M
authors.initials: VM
authors.lastname: Dixit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006493:Heparin / Q000378:metabolism; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019699:Thrombospondins; D014018:Tissue Distribution
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019699:Thrombospondins; D006493:Heparin