title: [A simultaneous determination of daidzin and puerarin and determination of daidzein in Oriental pharmaceutical decoctions containing puerariae radix by ion-pair high-performance liquid chromatography].
abstract: A simple and precise method was established for the simultaneous determination of daidzin and puerarin and the determination of daidzein in oriental pharmaceutical decoctions containing Puerariae Radix using high-performance liquid chromatography with tetra-n-heptylammonium bromide (THA) as an ion-pair reagent. Daidzin and puerarin were eluted within 45 min without interference with co-existing components using an ODS column and a mixture of 10 mM phosphate buffer (pH 6.5)-methanol (68:32) containing 5 mM THA as a mobile phase. Daidzein was eluted within 35 min without interference with co-existing components using an ODS column and a mixture of 10 mM phosphate buffer (pH 6.5)-acetonitrile (72:28 or 68:32) containing 5 mM THA as a mobile phase.
authors.affiliation: Tohoku College of Pharmacy, Miyagi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: N
authors.initials: N
authors.lastname: Momma
authors.forename: K
authors.initials: K
authors.lastname: Ohsawa
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C013908:daidzin; C033607:puerarin