Suppression of delayed-type hypersensitivity (DTH) responses in xenografts by pretreatment with ultraviolet (UV)-irradiated hepatocytes.
F344 Rat hepatocytes (HCs) that had been exposed to ultraviolet (UV) light were transplanted into the dorsal subcutaneous tissues of Balb/c mice. Four days after the transplantation, the anti-HC delayed-type hypersensitivity (DTH) response was assessed by determining the response to a direct challenge with non-irradiated HCs. The DTH response in mice transplanted with 600 J/m2-UVB-irradiated HCs was suppressed significantly compared with that with non-irradiated HCs. Furthermore, the DTH responses evoked by challenge with non-irradiated HCs were similar to those evoked by UV-irradiated HCs.
Tanabe
S
S
Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, Japan.
Taura
Y
Y
Tanaka
M
M
Nakaichi
M
M
Nakama
S
S
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006968:Hypersensitivity, Delayed / Q000517:prevention & control*; D008099:Liver / Q000528:radiation effects*; D016031:Liver Transplantation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011916:Rats, Inbred F344; D014183:Transplantation, Heterologous / Q000276:immunology*; D014466:Ultraviolet Rays