Changes of gastric mucosal biochemistry in ethanol-treated rats with and without acute surgical vagotomy.
The biochemical background of ethanol-(ETOH) induced gastric mucosal damage was studied in rats with intact vagus and after acute surgical vagotomy. Observations were carried out on Sprague-Dawley (CFY) strain rats of both sexes. Gastric mucosal lesions were produced by intragastric administration of 1 ml 96% ethanol. Bilateral truncal surgical vagotomy was carried out 30 min before ETOH administration. The number and severity of gastric mucosal lesions was noted 1 h after ETOH administration. Biochemical measurements (gastric mucosal level of ATP, ADP, AMP, cAMP and lactate) were carried out from the total homogenized gastric mucosa. The adenylate pool (ATP + ADP + AMP), energy charge ((ATP + 0.5 ADP)/(ATP + ADP + AMP)) and ratio of ATP/ADP were calculated. It was found that: 1) ATP transformation into ADP increased, while ATP transformation in cAMP decreased in ethanol-treated animals with intact vagus nerve, while these transformations were quite the opposite in vagotomized animals: 2) no significant changes were found in the tissue level of lactate: and 3) the extent of biochemical changes was significantly less after surgical vagotomy. It is concluded that an intact vagus is basically necessary for the metabolic adaptation of gastric mucosa.
Vincze
A
A
First Department of Medicine, Medical University of Pécs, Hungary.
Király
A
A
Sütö
G
G
Mózsik
G
G
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D000431:Ethanol; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D008297:Male; D051381:Rats; D013272:Stomach Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D014628:Vagotomy; D014630:Vagus Nerve / Q000503:physiopathology*
D000227:Adenine Nucleotides; D000431:Ethanol