Identification and sequence determination of the capsid protein gene of human astrovirus serotype 1.
We present the sequence of an open reading frame (ORF) at the 3' end of human astrovirus serotype 1. Primer extension experiments showed that the RNA expressing this gene is shorter than the complete ORF, and could form a protein of M(r) 85,540. The protein was expressed by recombinant baculovirus and was recognized by anti-virion serum, indicating a structural role. Sequence comparison indicates that astrovirus serotypes 1 and 2 differ markedly in the C-terminal half of the protein but are well conserved towards the N-terminus.
Willcocks
M M
MM
Division of Virology, School of Pathological Sciences, Medical School, University of Newcastle-upon-Tyne, UK.
Carter
M J
MJ
D000595:Amino Acid Sequence; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002213:Capsid / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005814:Genes, Viral / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D001257:Mamastrovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015531:Radioimmunoprecipitation Assay; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D015678:Viral Structural Proteins / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins