Discovery of D-amino-acid-containing ligands with selectide technology.
When all L-amino acid (aa) random peptide libraries synthesized on solid-phase particles were screened (Selectide Technology), we identified several peptide ligands (YG_F_) that interacted specifically with an anti-beta-endorphin monoclonal antibody (clone 3E7) (single-letter aa symbols; symbols '_' indicate variable aa). Here, we report on the screening of three different D-aa-containing pentapeptide libraries (XxXxX, xXxXx and xxxxx, wherein X = L-aa, and x = D-aa) with the same antibody, in which several D-aa-containing ligands were identified. The binding affinities of many of these D-aa-containing ligands were as least two orders of magnitude lower than that of YGGFL, for which the Ki is 17.5 nM.
Lam
K S
KS
Arizona Cancer Center, Tucson.
Lebl
M
M
Krchnak
V
V
Wade
S
S
Abdul-Latif
F
F
Ferguson
R
R
Cuzzocrea
C
C
Wertman
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000138:chemical synthesis*; D000818:Animals; D000911:Antibodies, Monoclonal; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D011897:Random Allocation; D001615:beta-Endorphin / Q000276:immunology
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D008024:Ligands; D001615:beta-Endorphin