Significance of pH on differentiation of human erythroid cell lines.
As physiological factors and compounds, BSA and/or higher pH of the culture medium could induce the erythroid differentiation of cells. Optimum pH values of the culture medium for higher spontaneous differentiation of KU-812 and K562 cells after 7 days cultivation were 7.5 and 7.6, respectively. The synergistic effects on the differentiation were observed by exposure to hemin under the higher pH condition. In the presence of BSA, 25% of KU-812 and 40% of K562 cells became benzidine positive. Synergistic effects of BSA and the higher pH of the medium were also observed.
Endo
T
T
Department of School-Nurse Training, Kyushu Women's Junior College, Japan.
Ishibashi
Y
Y
Okana
H
H
Fukumaki
Y
Y
D002454:Cell Differentiation; D003470:Culture Media; D004912:Erythrocytes / Q000737:chemistry / Q000473:pathology*; D006427:Hemin / Q000494:pharmacology; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000473:pathology
D003470:Culture Media; D006454:Hemoglobins; D012710:Serum Albumin, Bovine; D006427:Hemin