Evidence for the presence of a high-affinity laminin receptor-like molecule on the surface of Candida albicans yeast cells.
Two polypeptides of 37 and 67 kDa that bind laminin were detected in cell wall extracts of Candida albicans blastoconidia. The 37-kDa species, found only in yeast cell wall extracts, cross-reacted with a rabbit polyclonal antibody (PAb 4160) directed towards the carboxyl-terminal laminin-binding domain present in the human 67-kDa high-affinity laminin receptor (67LR) and its 37-kDa precursor (37LRP), whereas another antibody (PAb 4056), directed against internal domains of 67LR and 37LRP, recognized a 37-kDa species in wall extracts from both blastoconidia and germinated blastoconidia. Indirect immunofluorescence with PAb 4160 showed a patchy binding pattern only on yeast cells that represented about 10% of the entire blastoconidia population.
López-Ribot
J L
JL
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79430.
Casanova
M
M
Monteagudo
C
C
Sepúlveda
P
P
Martínez
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D000908:Antibodies, Fungal; D001665:Binding Sites; D002176:Candida albicans / Q000276:immunology / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D002473:Cell Wall / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D017474:Receptors, Laminin / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D000908:Antibodies, Fungal; D009842:Oligopeptides; D017474:Receptors, Laminin; C047981:arginyl-glycyl-aspartic acid