Cholinergic amacrine cells of the rat retina express the delta-subunit of the GABAA-receptor.
Antibodies directed against the delta-subunit of the GABAA-receptor were applied to cryostat sections of rat retinae. Two narrow bands of the inner plexiform layer were strongly immunoreactive. Some cell bodies in both the amacrine- and ganglion-cell layer were weakly immunoreactive. The position of the labelled bands and the distribution of the cell bodies was strongly reminiscent of the cholinergic amacrine cells. In order to show directly that cholinergic amacrine cells express the delta-subunit of the GABAA-receptor, double immunofluorescence with an antibody against choline acetyltransferase (ChAT) and with antibodies against the delta-subunit was performed on the same cryostat sections. This showed the labelled cells to be cholinergic amacrine cells.
Greferath
U
U
Max-Planck-Institut für Hirnforschung, Frankfurt am Main, FRG.
Grünert
U
U
Möhler
H
H
Wässle
H
H
D000818:Animals; D002795:Choline O-Acetyltransferase / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000276:immunology; D012160:Retina / Q000166:cytology / Q000378:metabolism*
D011963:Receptors, GABA-A; D002795:Choline O-Acetyltransferase