Localization by in situ hybridization of mRNAs encoding stromelysin 3 and tissue inhibitors of metallo-proteinases TIMP-1 and TIMP-2 in human head and neck carcinomas.
The presence and distribution of mRNAs encoding a matrix metalloproteinase (MMP) stromelysin 3 and two tissue inhibitors of MMP, TIMP1 and TIMP-2 have been studied by in situ hybridization of 18 human epidermoid head and neck carcinomas and four normal tissues. We found that in 16 tumors out of 18, stromelysin 3 mRNAs were only expressed by fibroblasts which were in close contact to invasive cancer cells. Tumor cells and normal tissues were not labeled. TIMP-1 mRNAs were detected in well differentiated cancer cells and in endothelial cells in all the cancers. In 13 out of the 18 carcinomas, TIMP-2 mRNAs were localized in only a few stromal cells near well differentiated invasive cancer cells and in endothelial cells. The significant expression of TIMP-1 and TIMP-2 mRNAS may lead to less aggressive MMPs, especially in the case of stromelysin 3, in the invasive process of the stroma.
Polette
M
M
I.N.S.E.R.M. U-314, Université de Reims, France.
Clavel
C
C
Birembaut
P
P
De Clerck
Y A
YA
D015152:Blotting, Northern; D002294:Carcinoma, Squamous Cell / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000737:chemistry*; D006801:Humans; D017403:In Situ Hybridization; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D011506:Proteins; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases