In vivo and in vitro analyses of recombinant baculoviruses lacking a functional cg30 gene.
The cg30 gene of Autographa californica nuclear polyhedrosis virus (AcMNPV) encodes two sequence motifs, a zinc finger-like motif and a leucine zipper, found in other polypeptides known to be involved in gene regulation. To gain insight into the function of the cg30 product, CG30, we constructed and characterized recombinant viruses lacking a functional cg30 gene. We found that cg30 mutants had no striking phenotype in cell lines derived from Spodoptera frugiperda or Trichoplusia ni or in T. ni larvae. Although cg30 is known to be transcribed as an early monocistronic RNA and as the second cistron of an abundant late bicistronic RNA, production of a CG30-beta-galactosidase fusion protein was observed mainly at early times postinfection. Viruses containing cg30 had a subtle growth advantage over those lacking cg30 after several viral passages in cell culture. We employed transient expression assays to determine whether cg30 and pe-38, an AcMNPV gene that encodes a polypeptide with zinc finger-like and leucine zipper motifs similar to those of cg30, have redundant functions. Although pe-38 may have a role in AcMNPV gene expression, there was no indication that cg30 and pe-38 are functionally redundant.
Passarelli
A L
AL
Department of Genetics, University of Georgia, Athens 30602.
Miller
L K
LK
D000483:Alleles; D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D005809:Genes, Regulator / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D007814:Larva / Q000382:microbiology; D009036:Moths / Q000166:cytology / Q000382:microbiology*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000472:pathogenicity; D012692:Serial Passage; D013997:Time Factors; D014764:Viral Proteins; D014774:Virulence
C085303:Ac-cg30 protein, Autographa californica nucleopolyhedrovirus; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D014764:Viral Proteins