Immunostimulant activity of a novel lipopeptide and its protective action against Leishmania donovani.
Novel lipopeptides 84/201 and 86/450 synthesized in this laboratory stimulated antibody and delayed type hypersensitivity (DTH) response to ovalbumin in guinea pigs. Lipopeptide 86/450 also stimulated antibody and DTH responses in albino mice and enhanced nonspecifically macrophage migration index (MMI), phagocytic activity and incorporation of [14C] glucosamine in peritoneal macrophages of the treated animals. Proliferative response of splenocytes from lipopeptide 86/450 treated animals was significantly higher than that from untreated controls. Peritoneal macrophages from lipopeptide 86/450 treated mice were less susceptible to Leishmania donovani promastigote invasion when co-cultured in vitro. The treated animals on challenge with L. donovani promastigote/amastigote showed 80 to 90% lower intake of infection than the control animals.
Puri
A
A
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Rizvi
S Y
SY
Haq
W
W
Guru
P Y
PY
Kundu
B
B
Saxena
R P
RP
Shukla
R
R
Mathur
K B
KB
Saxena
K C
KC
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D006224:Cricetinae; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D066298:In Vitro Techniques; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000517:prevention & control; D055666:Lipopeptides; D008074:Lipoproteins / Q000737:chemistry / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects
D000276:Adjuvants, Immunologic; D055666:Lipopeptides; D008074:Lipoproteins; C085680:lipopeptide 84-201; C085681:lipopeptide 86-450; D010047:Ovalbumin