Possible implications of Sp1-induced bending of DNA on synergistic activation of transcription.
We examined DNA bending using an electrophoretic mobility shift assay to determine whether Sp1 induces structural changes in DNA. The results indicated that Sp1 bends DNA upon binding to its recognition sequence. We discuss the possibility that DNA bending is involved in synergistic activation of transcription.
Ikeda
K
K
Department of Biology, Jichi Medical School, Tochigi, Japan.
Nagano
K
K
Kawakami
K
K
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D016329:Sp1 Transcription Factor / Q000494:pharmacology*; D014158:Transcription, Genetic
D016329:Sp1 Transcription Factor; D004247:DNA