Brefeldin A blocks protein glycosylation and RNA replication of vesicular stomatitis virus.
Brefeldin A is a macrolide antibiotic that interferes with membrane traffic and blocks the growth of several animal viruses including vesicular stomatitis virus (VSV). The inhibition of VSV by brefeldin A takes place at least at two different steps during the growth cycle: the glycosylation of VSV G protein and the replication of viral genomes. Our results indicate that interference with membrane traffic leads not only to inhibition of viral protein glycosylation, but also to the blockade of virus genome replication in several cytoplasmic RNA-containing viruses.
Irurzun
A
A
Centro de Biologia Molecular, Universidad Autónoma de Madrid, Spain.
Pérez
L
L
Carrasco
L
L
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D020126:Brefeldin A; D002460:Cell Line; D006224:Cricetinae; D003517:Cyclopentanes / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D012367:RNA, Viral / Q000096:biosynthesis*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification
D000998:Antiviral Agents; D003517:Cyclopentanes; D012367:RNA, Viral; D014764:Viral Proteins; D020126:Brefeldin A