2A proteinases of coxsackie- and rhinovirus cleave peptides derived from eIF-4 gamma via a common recognition motif.
The cleavage specificities of the 2A proteinases from coxsackievirus B4 (CVB4) and human rhinovirus 2 (HRV2) on oligopeptide substrates have been determined. Comparison of the specificity of CVB4 2A proteinase with that of HRV2 2A proteinase allowed cleavable peptides to be designed using the common motif IIe/Leu-X-Thr-X*Gly; little resemblance to the viral cleavage site remained. The data also allowed the prediction of three possible cleavage sites for 2A proteinases on eIF-4 gamma; two peptides derived from these sequences were cleaved by both 2A proteinases. One of these peptides corresponds to the cleavage site for 2A proteinases mapped on eIF-4 gamma [B. J. Lamphear et al. (1993) J. Biol. Chem. 268, 19200-19203]. This supports the hypothesis that cleavage of eIF-4 gamma by picornaviral 2A proteinases occurs directly.
Sommergruber
W
W
BENDER+CO Ges mbH, Ernst Boehringer Institut fuer Arzneimittelforschung, Vienna, Austria.
Ahorn
H
H
Klump
H
H
Seipelt
J
J
Zoephel
A
A
Fessl
F
F
Krystek
E
E
Blaas
D
D
Kuechler
E
E
Liebig
H D
HD
D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D029822:Enterovirus B, Human / Q000201:enzymology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000378:metabolism*; D012229:Rhinovirus / Q000201:enzymology*; D013045:Species Specificity; D013379:Substrate Specificity; D014764:Viral Proteins
D010446:Peptide Fragments; D010448:Peptide Initiation Factors; D014764:Viral Proteins; C077382:eIF-4gamma; D003546:Cysteine Endopeptidases; C057400:picornain 2A, Picornavirus