Effects of 2-mercaptoethanol on survival and differentiation of fetal mouse brain neurons cultured in vitro.
Effects of 2-mercaptoethanol on primary cultures of fetal mouse brain neurons have been investigated. The addition of 2-mercaptoethanol to the culture medium increased 6- or 200-fold the survival rates of embryonic day-16 murine striatum neurons and day-18 cerebral cortical neurons cultured in serum-free medium, respectively, and also induced neurite outgrowth, particularly being prominent in cortical neurons. Moreover, this drug enhanced trophic activities of the conditioned medium of VR-2g or BIM cells. These findings indicate that 2-mercaptoethanol can support the viability and differentiation of fetal mouse brain neurons.
Ishii
K
K
Department of Cell Biology, Kyoto University, Japan.
Katayama
M
M
Hori
K
K
Yodoi
J
J
Nakanishi
T
T
D000818:Animals; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003342:Corpus Striatum / Q000166:cytology; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D008623:Mercaptoethanol / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D009928:Organ Specificity
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D008623:Mercaptoethanol