Cloning and functional expression of a cardiac inward rectifier K+ channel.
We have isolated a cDNA coding for an inward rectifier K+ channel (RBHIK1) from rabbit heart. The cloned cDNA encodes a protein of 427 amino acids with two putative transmembrane segments. The primary structure of RBHIK1 is highly homologous to that of IRK1 which is an inward rectifier K+ channel recently cloned from mouse macrophage by expression cloning. When expressed in Xenopus oocytes, RBHIK1 current showed strong inward rectification and was inhibited by extracellular Ba2+ and Cs+. RNA blot analysis revealed the expression of RBHIK1 mRNA in various rabbit tissues, especially high level in the ventricular muscle.
Ishii
K
K
Department of Pharmacology, Tohoku University School of Medicine, Sendai, Japan.
Yamagishi
T
T
Taira
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004594:Electrophysiology; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D014981:Xenopus
D018076:DNA, Complementary; D015221:Potassium Channels; D012333:RNA, Messenger