title: Lack of intraspecific variation in the second internal transcribed spacer (ITS-2) of Trichostrongylus colubriformis ribosomal DNA.
abstract: The nucleotide sequence of the second internal transcribed spacer (ITS-2) was determined for three populations of the parasitic nematode Trichostrongylus colubriformis which differed in their susceptibility to benzimidazole anthelmintics and/or in their geographical origin. No intraspecific variation was found in the ITS-2 sequence, indicating that this region of rDNA is inadequate to discriminate between resistant and susceptible populations of T. colubriformis, but it may prove useful for distinguishing between species of Trichostrongylus.
authors.affiliation: INTRA-CR Tours, Station de Pathologie aviaire et de Parasitologie, Nouzilly, France.
authors.forename: H
authors.initials: H
authors.lastname: Hoste
authors.forename: R B
authors.initials: RB
authors.lastname: Gasser
authors.forename: N B
authors.initials: NB
authors.lastname: Chilton
authors.forename: S
authors.initials: S
authors.lastname: Mallet
authors.forename: I
authors.initials: I
authors.lastname: Beveridge
mesh_terms: D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D008969:Molecular Sequence Data; D014254:Trichostrongylus / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal