In situ localization of the 60 k protein of Helicobacter pylori, which belongs to the family of heat shock proteins, by immuno-electron microscopy.
The groEl homologue of Helicobacter pylori was isolated and characterized by means of immunoelectron microscopy, after cryosectioning. The 60 k protein was isolated from Helicobacter pylori by treatment of the cells with 2-butanol and purified by anion exchange chromatography. The native molecular weight of the 60 k protein was estimated to be 420 k by size exclusion chromatography. The purified 60 k protein showed the typical rotational symmetry of chaperonins when analyzed by electron microscopy. Ultrathin sections of Helicobacter pylori were immunostained by a polyclonal antibody directed against the hsp-65 of Mycobacterium tuberculosis. The label revealed a clustered localization of the 60 k protein on the cell surface as well as in the periplasmic space.
Eschweiler
B
B
Institute for Medical Microbiology, Freiburg, Germany.
Bohrmann
B
B
Gerstenecker
B
B
Schiltz
E
E
Kist
M
M
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000648:ultrastructure; D018834:Chaperonin 60; D018833:Chaperonins; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D016480:Helicobacter pylori / Q000737:chemistry* / Q000648:ultrastructure; D015151:Immunoblotting; D017403:In Situ Hybridization; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000276:immunology; D017386:Sequence Homology, Amino Acid
C078096:60K protein, Helicobacter pylori; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins