title: Avidin-biotin based electrochemical immunoassay for thyrotropin.
abstract: A two site electrochemical enzyme immunoassay for thyrotropin (TSH) has been developed. This assay is based on the use of an immobilized capture antibody and a biotinylated second antibody. Detection is achieved via avidin labelled with alkaline phosphatase. The substrate 1-naphthyl phosphate was used and the product 1-naphthol was detected at disposable screen-printed carbon 8-electrode combs using specially designed instrumentation.
authors.affiliation: Department of Clinical Biochemistry, Medical School, University of Newcastle Upon Tyne, UK.
authors.forename: D
authors.initials: D
authors.lastname: Athey
authors.forename: M
authors.initials: M
authors.lastname: Ball
authors.forename: C J
authors.initials: CJ
authors.lastname: McNeil
mesh_terms: D000469:Alkaline Phosphatase; D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D004563:Electrochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013972:Thyrotropin / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D013972:Thyrotropin; D000469:Alkaline Phosphatase