Immunohistological study on bovine small intestine including gut associated lymphoid tissue using monoclonal antibodies to WC1, TCR1 and BoCD3.
Selected antibodies (mAbs) that clustered in temporary cluster TC22 (WC1) and TC36 (TCR1) and one unclustered mAb thought to detect BoCD3 were used to compare the distribution of T and B cells and subpopulations of WC1+ or TCR1+ cells in fetal, calf, and young adult gut associated lymphoid tissue. Cells putatively positive for BoCD3 (MM1A) were detected in the interfollicular tissue similar to the pattern noted for BoCD2+ or BoCD5+ cells. WC1+ or TCR1+ cells were detected in small numbers in follicles and interfollicular tissue. Numerous WC1+ or TCR1+ cells were evident in the lamina propria and epithelia, with the highest frequency evident in tissues from animals 8 months of age.
Okada
K
K
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Japan.
Suzuki
K
K
Murakami
K
K
Davis
W C
WC
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007413:Intestinal Mucosa / Q000166:cytology; D007421:Intestine, Small / Q000166:cytology* / Q000276:immunology; D016131:Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D010581:Peyer's Patches / Q000166:cytology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016692:Receptors, Antigen, T-Cell, gamma-delta