[Determination of the folic acid content of grain, cereal products, baked goods and legumes using high-performance liquid chromatography (HPLC)].
The folic acid content of grain, cereal products (including beer), bakery products and legumes was determined by means of high-performance liquid chromatography (HPLC). Free folate (monoglutamate forms) and total folate (monoglutamate + polyglutamate forms) were differentiated. Of the grain analysed, rye, with a mean value of 143 micrograms/100 g, contained more total folate than wheat (mean = 91 micrograms/100 g). The total folate content of bakery products ranged from 14 micrograms/100 g (whole grain rye bread) to 88 micrograms/100 g (crispbread). Beer had a very low total folate content (mean = 3 micrograms/100 ml). The mean of the free folate portion was 76.5% in grain and cereal products and 65.6% in bakery products. Of the legumes analysed, beans (mean = 128 micrograms/100 g) had the highest content of total folate, followed by lentils (103 micrograms/100 g) and peas (57 micrograms/100 g). The mean value of the free folate portion in legumes (73.1%) was comparable with the values of grain, cereal products and bakery products. In addition to tetrahydrofolic acid (THF), 5-methyl-THF and 5-formyl-THF, pteroylglutamic acid (PteGlu) and 10-formyl-PteGlu were determined in all products (except beer). Their proportion (PteGlu + 10-formyl-PteGlu) of the total folate content ranged from 23.5% to 44.4%.
Müller
H
H
Institut für Ernährungsphysiologie der Bundesforschungsanstalt für Ernährung, Karlsruhe, Deutschland.
D001515:Beer / Q000032:analysis; D001939:Bread / Q000032:analysis; D002523:Edible Grain / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry*; D005492:Folic Acid / Q000032:analysis*; D002955:Leucovorin / Q000031:analogs & derivatives / Q000032:analysis; D010946:Plants, Medicinal; D012434:Secale / Q000737:chemistry; D013763:Tetrahydrofolates / Q000032:analysis; D014908:Triticum / Q000737:chemistry
D013763:Tetrahydrofolates; C010161:10-formyltetrahydropteroylglutamic acid; C030371:5,6,7,8-tetrahydrofolic acid; D005492:Folic Acid; D002955:Leucovorin; C005984:5-methyltetrahydrofolate
Bestimmung der folsäure-Gehalte von Getreide, Getreideprodukten, Backwaren und Hülsenfrüchten mit Hilfe der Hochleistungsflüssigchromatographie (HPLC).