Interference between cold-adapted (ca) influenza A and B vaccine reassortants or between ca reassortants and wild-type strains in eggs and mice.
Interference between pairs of cold-adapted (ca) influenza A and B vaccine reassortant viruses and between ca influenza B reassortant viruses and wild-type influenza strain A/PR/8/34 was studied in embryonated eggs and/or in the respiratory tract of three strains of mice. Interference in eggs between ca A and B reassortants occurred after 2 days but was masked by later cycles of growth. When doses of 10(6) EID50 of the reassortant viruses B/60/32/R and A/47/6/R were administered to outbred mice, interference with the growth of A/47/6/R occurred in the turbinates; interference with the growth of B/60/32/R did not occur. However, interference with the growth of B/60/32/R was noted in the lungs of Balb/c mice after co-infection with 10(6) EID50 of both A/PR/8/34 and B/60/32/R. Interference did not occur when the dose of B/60/32/R was increased to 10(7) EID50 per mouse. When CBA mice were co-infected with 10(6) EID50 of both B/60/32/R and the influenza A ca reassortant R/34, or 10(6) EID50 of B/60/32/R,R/34 and A/Leningrad/134/57, interference did not occur. Interference could not be detected in mice following dual infection from serum responses to individual viruses.
Romanova
J R
JR
Department of Virology, Russian Academy of Medical Sciences, St Petersburg.
Ermachenko
T A
TA
Alexandrova
G I
GI
Tannock
G A
GA
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D004531:Eggs / Q000382:microbiology*; D009980:Influenza A virus / Q000502:physiology*; D009981:Influenza B virus / Q000502:physiology*; D007252:Influenza Vaccines / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009976:Orthomyxoviridae Infections / Q000382:microbiology; D016865:Reassortant Viruses / Q000502:physiology*; D014762:Viral Interference / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology
D000914:Antibodies, Viral; D007252:Influenza Vaccines