title: Testosterone-induced changes in phosphatidylcholine molecular species composition of Plasmodium chabaudi-infected erythrocytes.
abstract: This study is concerned with the influence of testosterone on the phospholipid class and the phosphatidylcholine molecular species composition of various fractions obtained from the blood of Plasmodium chabaudi-infected mice. Blood plasma, infected erythrocytes, isolated parasites and erythrocyte membranes isolated from both non-infected and infected erythrocytes in the form of ghosts were analysed. In general, the phospholipid classes remained unaffected, while the phosphatidylcholine (PC) molecular species composition showed differences after testosterone treatment. In infected erythrocytes, there was a decrease in 16:0/20:4-PC and 18:0/20:4-PC and an increase in 16:0/18:2(16:0/20:3)-PC. The decrease of 16:0/20:4-PC was exclusively confined to parasites. The rise in 16:0/18:2(16:0/20:3)-PC and the diminution of 18:0/20:4-PC occurred in the erythrocyte membrane of both infected ghosts and non-infected ghosts as well as in the blood plasma. It is suggested that these changes occur primarily in the plasma thereby influencing the erythrocyte membranes. The decrease in 16:0/20:4-PC supports the view of the independence of the parasite from the biosynthetic lipid pathways of its host cell.
authors.affiliation: Division of Parasitology, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Fiebig
authors.forename: A P
authors.initials: AP
authors.lastname: Simões
authors.forename: F
authors.initials: F
authors.lastname: Wunderlich
authors.forename: J A
authors.initials: JA
authors.lastname: op den Kamp
mesh_terms: D000818:Animals; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology*; D005260:Female; D008288:Malaria / Q000097:blood*; D051379:Mice; D008815:Mice, Inbred Strains; D010713:Phosphatidylcholines / Q000097:blood / Q000378:metabolism*; D010743:Phospholipids / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D016788:Plasmodium chabaudi / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology*; D012016:Reference Values; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D010713:Phosphatidylcholines; D010743:Phospholipids; D013739:Testosterone