The gene encoding mouse lymphocyte antigen Ly-49: structural analysis and the 5'-flanking sequence.
Genomic clones encoding the mouse cell-surface antigen, Ly-49, were isolated, and the gene organization was analyzed. The gene spanned approximately 19 kb, and contained seven exons and six introns. The lengths of introns ranged from 1.3 to 8 kb. A 1067-bp sequence in the 5'-flanking region was determined. Primer extension analysis and S1 nuclease mapping revealed a cap site at 158 bp upstream from the ATG coding the N-terminal Met of Ly-49. The 5'-flanking sequence contained a possible promoter sequence, a potential binding site for the T cell-specific transcription factor (TCF-1 alpha/LEF-1), and three sites for the basic helix-loop-helix-binding basic proteins (bHLH). However, no CAAT box-like sequence was present. These results provide important clues for understanding the mechanism of gene expression of lymphocyte antigens.
Kubo
S
S
Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
Itoh
Y
Y
Ishikawa
N
N
Nagasawa
R
R
Mitarai
T
T
Maruyama
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000950:Antigens, Ly / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D012315:RNA Caps; D012333:RNA, Messenger / Q000235:genetics
D000950:Antigens, Ly; D018076:DNA, Complementary; D012315:RNA Caps; D012333:RNA, Messenger