Refinement of the localization of the gene for human intraacrosomal protein SP-10 (ACRV1) to the junction of bands q23-->q24 of chromosome 11 by nonisotopic in situ hybridization.
The human sperm antigen SP-10 is a testis-specific protein associated with the matrix of the acrosomal vesicle in developing spermatids and the acrosomal matrix and membranes of ejaculated sperm. A previous study, utilizing somatic cell hybrids, localized the gene for SP-10 to chromosome 11 and assigned the gene symbol ACRV1 (acrosomal vesicle protein-1). Although previous analysis of several somatic cell hybrids containing portions of chromosome 11 indicated that ACRV1 was in the p12-->q13 region, the present fluorescence in situ hybridization studies using cDNA, ribo, and genomic versions of probes for SP-10 coupled to analysis of an expanded series of somatic cell hybrids demonstrated the refined localization of ACRV1 to the junction of bands q23 and q24 of chromosome 11. A comparison of the three types of probes used for the in situ study demonstrated that while the genomic probe hybridized most efficiently, the riboprobe hybridized to the same location and was superior to the cDNA probe in mapping this single-copy gene. This report emphasizes the utility of riboprobes for chromosome localization of single-copy genes.
Golden
W L
WL
Department of Pediatrics, University of Virginia, Charlottesville 22908.
von Kap-Herr
C
C
Kurth
B
B
Wright
R M
RM
Flickinger
C J
CJ
Eddy
R
R
Shows
T
T
Herr
J C
JC
D000177:Acrosome / Q000378:metabolism*; D000941:Antigens / Q000235:genetics*; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D012739:Gonadal Steroid Hormones / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins
C512729:ACRV1 protein, human; D000941:Antigens; D012739:Gonadal Steroid Hormones; D008565:Membrane Proteins