[Synthesis and antigenic activity of peptides from the C-terminal part of the NS4-protein of the hepatitis A virus].
A set of four peptides from the HCV NS4-protein C-terminal region (aa 1921-1940) were obtained by solid-phase synthesis using activated esters and symmetrical anhydrides of Boc-amino acids. Peptide 1921-1940 has demonstrated a positive reaction in ELISA with individual anti-HCV-positive sera from patients with acute and chronic hepatitis C (80% and 56%, respectively). We analysed the antigenic properties of the peptide 1921-1940 and its fragments and suggested at least two antibody recognizing sites to be contained in this region.
Semiletov
Iu A
IuA
Firsova
T V
TV
Kuzin
S N
SN
Khudiakov
Iu E
IuE
Shibnev
V A
VA
D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000737:chemistry*; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
D000956:Antigens, Viral; D010446:Peptide Fragments; D017361:Viral Nonstructural Proteins
Sintez i antigennaia aktivnost' peptidov iz C-kontsevoĭ chasti nestrukturnogo NS4-belka virusa gepatita C.