Demonstration of circulating pneumococcal immunoglobulin G immune complexes in patients with community-acquired pneumonia by means of an enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay was developed for quantitation of circulating immune complexes (CICs) containing specific antipneumococcal immunoglobulin G (IgG). These CICs were detected in 17 (85%) of 20 patients with bacteremic pneumococcal pneumonia, 4 (36.4%) of 11 patients with probable pneumococcal pneumonia, 3 (16.7%) of 18 patients with pneumonia of other (nonpneumococcal) etiology, and 13 (41.9%) of 31 patients with pneumonia of unknown etiology. There was no correlation between CICs and serum IgG antibody levels. Pneumococcal capsular antigen was demonstrated in dissociated CICs by latex agglutination.
Holloway
Y
Y
Immunology Research, Regional Public Health Laboratory, Groningen, The Netherlands.
Snijder
J A
JA
Boersma
W G
WG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000097:blood*; D000942:Antigens, Bacterial / Q000097:blood; D016470:Bacteremia / Q000276:immunology; D017714:Community-Acquired Infections / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007841:Latex Fixation Tests; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000276:immunology; D011018:Pneumonia, Pneumococcal / Q000276:immunology*; D012015:Reference Standards; D013296:Streptococcus pneumoniae / Q000276:immunology
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D007074:Immunoglobulin G