title: Rapid and sensitive gas chromatographic determination of estazolam.
abstract: The triazolobenzodiazepine estazolam can be quantitated by gas chromatography with electron-capture detection. After addition of a suitable internal standard, plasma samples are extracted into toluene-isoamyl alcohol or benzene-isoamyl alcohol. The organic extract is separated, evaporated to dryness, reconstituted, and chromatographed using a 50:50 methyl-phenyl column (SP-2250). The sensitivity limit is approximately 1 ng of estazolam in a 1-ml sample. The method is suitable for clinical or experimental pharmacokinetic studies.
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Boston, MA 02111.
authors.forename: J F
authors.initials: JF
authors.lastname: Kelly
authors.forename: D J
authors.initials: DJ
authors.lastname: Greenblatt
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D004949:Estazolam / Q000097:blood*; D008297:Male; D051379:Mice
chemical_list: D004949:Estazolam