Total loss of p53 DNA sequences in acute myeloid leukemia.
Mutations of the p53 tumour suppressor gene on chromosome 17p are a common genetic change in the malignant progression of many cancers. Here we report a case of a 71-year-old man with haematological, cytofluorimetric and cytochemical findings consistent with a 'de novo' M2 acute myeloid leukaemia (AML). A complex karyotype including a whole chromosome 17 and a t(17;?) (p11;?) was present in 8 of 10 metaphases of bone marrow cells. Southern blot analysis of the bone marrow DNA showed a specific loss of p53 gene in the AML cells. As far as we know, this is the first report of a deletion of both p53 alleles in leukaemia. The effect of the loss of p53 on the course of AML is discussed.
Gandini
D
D
Centro Studi Biochimici delle Patologie del Genoma Umano, Ferrara, Italy.
Cuneo
A
A
Carli
M G
MG
Lanza
F
F
Castoldi
G L
GL
del Senno
L
L
D000368:Aged; D000483:Alleles; D002886:Chromosomes, Human, Pair 17; D004273:DNA, Neoplasm / Q000032:analysis*; D017353:Gene Deletion; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male
D004273:DNA, Neoplasm