Effect of follicle-stimulating hormone and luteinizing hormone during bovine in vitro maturation on development following in vitro fertilization and nuclear transfer.
The effects of luteinizing hormone (LH) (0, 100, 10,000 IU/ml) and follicle-stimulating hormone (FSH) (20 micrograms/ml) supplementation during in vitro maturation of slaughterhouse-derived oocytes on polar body formation and embryo development subsequent to in vitro fertilization and nuclear transfer were evaluated. Gonadotropin supplementation of maturation medium in the presence of serum neither enhanced the proportion of oocytes forming a polar body nor significantly affected development following in vitro fertilization or nuclear transfer, except at the highest LH concentration. A very high concentration of LH (10,000 IU/ml) significantly decreased polar body formation, initial cleavage, and blastocyst development (P < 0.05).
Keefer
C L
CL
ABS Specialty Genetics, DeForest, WI 53532.
Stice
S L
SL
Dobrinsky
J
J
D000818:Animals; D002417:Cattle; D003470:Culture Media; D046508:Culture Techniques; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development
D003470:Culture Media; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone