In vitro characterization of a phosphate starvation-independent carbon-phosphorus bond cleavage activity in Pseudomonas fluorescens 23F.
A novel, metal-dependent, carbon-phosphorus bond cleavage activity, provisionally named phosphonoacetate hydrolase, was detected in crude extracts of Pseudomonas fluorescens 23F, an environmental isolate able to utilize phosphonoacetate as the sole carbon and phosphorus source. The activity showed unique specificity toward this substrate; its organic product, acetate, was apparently metabolized by the glyoxylate cycle enzymes of the host cell. Unlike phosphonatase, which was also detected in crude extracts of P. fluorescens 23F, phosphonoacetate hydrolase was inducible only in the presence of its sole substrate and did not require phosphate starvation.
McMullan
G
G
School of Biology and Biochemistry, Queen's University, Belfast, Northern Ireland.
Quinn
J P
JP
D002244:Carbon / Q000172:deficiency / Q000378:metabolism; D004790:Enzyme Induction; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006867:Hydrolases / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000096:biosynthesis; D008670:Metals / Q000494:pharmacology; D063065:Organophosphonates / Q000494:pharmacology; D010746:Phosphonoacetic Acid / Q000378:metabolism*; D010758:Phosphorus / Q000172:deficiency / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013379:Substrate Specificity; D000097797:Glyphosate
D002244:Carbon; D005998:Glycine; D006867:Hydrolases; D007522:Isocitrate Lyase; D008670:Metals; D063065:Organophosphonates; D010746:Phosphonoacetic Acid; D010758:Phosphorus; D000097797:Glyphosate; C023920:phosphonoacetaldehyde hydrolase