Computer-assisted 3D analysis of cell distributions in the normal and epileptic cerebral cortex: description of a methodology in progress.
This paper describes software routines that (a) visualizes a stack of several thousands of aligned sequential photographic two-dimensional (2D) images stored in an image processing system; (b) creates a data base containing information about objects identified sequentially from the 2D images; (c) transfers the data base to a graphical terminal; (d) reconstructs a three-dimensional (3D) object space; and (e) supports on-line interaction between the image processing system and the graphical terminal. As an application example, the cell content of a prism of motor cerebral cortex of the cat is reconstructed. Preliminary results from reconstructing human epileptic temporal cortex (cortical microdysgenesia) are also reported.
Pascher
R
R
MedNet-laboratory, Medical Faculty, Göteborg University, Sweden.
Berthold
C H
CH
Rydmark
M
M
Skoglund
T
T
Jansson
T
T
Gustavsson
T
T
D000328:Adult; D000818:Animals; D002415:Cats; D002452:Cell Count; D002540:Cerebral Cortex / Q000166:cytology*; D003196:Computer Graphics; D004827:Epilepsy / Q000473:pathology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009044:Motor Cortex / Q000166:cytology; D012984:Software