Production of interleukin-1 by primary cultured parenchymal liver cells (hepatocytes).
The production of interleukin-1 (IL-1) by cultured parenchymal liver cells was revealed by a biological assay with an IL-1-dependent cell line, Northern blot analysis, and in situ hybridization. Inhibition experiments on the IL-1 activity with anti IL-1 alpha antibody also support the presence of IL-1 alpha in the supernatant of cultured parenchymal liver cells. Based on these results, we discuss the possibility of IL-1 production by parenchymal liver cells in vivo.
Tsukui
T
T
Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan.
Kikuchi
K
K
Mabuchi
A
A
Sudo
T
T
Sakamoto
T
T
Asano
G
G
Yokomuro
K
K
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001681:Biological Assay; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004247:DNA / Q000096:biosynthesis; D015342:DNA Probes; D005260:Female; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D008099:Liver / Q000378:metabolism*; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism
D000906:Antibodies; D017077:Culture Media, Conditioned; D015342:DNA Probes; D007375:Interleukin-1; D004247:DNA