Different forms of 130 kD connective tissue protein are specific for boundaries in the nervous system and basement membrane of muscle cells in leech.
The nervous system and muscle tissue of the leech express two different organ-specific forms of connective tissue protein. The nervous system-specific form appears in regional boundaries separating cell bodies, axonal tracts and areas of the neuropile during late embryogenesis. In contrast, the muscle-specific form appears earlier during development in the basement membrane of muscle cells. In extraction experiments both forms behave like extracellular matrix proteins and because of their molecular weight, are considered members of a group of cell type-specific 130 kD proteins (leech gp130s). However, the two forms differ in their posttranslational modification. As determined by Con A and lentil lectin affinity chromatography, only the nervous system-specific, but not the muscle-specific form, has fucosylated and high mannose N-linked carbohydrates. These differences in the developmental onset and glycosylation suggest that nervous system-specific and muscle-specific connective tissue proteins are regulated differently and participate in different molecular interactions.
Thorey
I
I
Abteilung fuer Haematology, Universitaetsklinik, Frankfurt/M.
Zipser
B
B
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002846:Chromatography, Affinity; D003238:Connective Tissue / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian; D005109:Extracellular Matrix / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D037102:Lectins; D007865:Leeches / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D009420:Nervous System / Q000378:metabolism*; D016613:Plastic Embedding; D011499:Protein Processing, Post-Translational
D037102:Lectins