Development of a genetic system for Eikenella corrodens: transfer of plasmids pFM739 and pLES2.
Eikenella corrodens is a Gram-negative microaerophilic rod which is emerging as an important human pathogen. Elucidation of the mechanisms by which it causes disease require efficient methods for the transfer of DNA to E. corrodens. Plasmids pFM739 and pLES2 have been transferred by conjugation from Escherichia coli S17-1 to E. corrodens ATCC 23834 at frequencies of 2.5 x 10(-7) and 2.42 x 10(-7), respectively. In addition, both plasmids could be transferred to four additional, clinical strains of E. corrodens at a similar frequency. The use of bacteriophage T4 as a counterselecting agent is also described.
Rao
V K
VK
Department of Oral Biology, University of Florida, Gainesville 32610-0424.
Whitlock
J A
JA
Progulske-Fox
A
A
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000378:metabolism; D016124:Eikenella corrodens / Q000235:genetics* / Q000472:pathogenicity; D004926:Escherichia coli / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D010957:Plasmids; D013045:Species Specificity
D004269:DNA, Bacterial