title: 1,25-dihydroxyvitamin D3 potentiates fluoride-stimulated collagen type I production in cultures of human bone marrow stromal osteoblast-like cells.
abstract: In this study we tested the effects of sodium fluoride (NaF) in serum-free cultures of human marrow stromal osteoblast-like [hMS(OB)] cells. NaF (10(-5) M) stimulated hMS(OB) cell proliferation up to 220% of control cultures. NaF alone did not increase type I collagen production, but in the presence of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] (10(-9) M), NaF enhanced type I collagen production in a dose-dependent way to 300% of 1,25-(OH)2D3-treated control cultures. The production of alkaline phosphatase (ALP) and osteocalcin (bone gla protein, BGP) was also enhanced in the presence of 1,25-(OH)2D3 to 170 and 200%, respectively, of 1,25-(OH)2D3-treated controls. Our results suggest that 1,25-(OH)2D3 potentiates fluoride-mediated anabolism in hMS(OB) cell cultures and suggest that osteoblast precursors in bone marrow are targets for fluoride action.
authors.affiliation: University Department of Endocrinology and Metabolism, Aarhus amtssygehus, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Kassem
authors.forename: L
authors.initials: L
authors.lastname: Mosekilde
authors.forename: E F
authors.initials: EF
authors.lastname: Eriksen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002117:Calcitriol / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D015675:Osteocalcin / Q000096:biosynthesis; D010446:Peptide Fragments / Q000096:biosynthesis; D011347:Procollagen / Q000096:biosynthesis; D012969:Sodium Fluoride / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects
chemical_list: D010446:Peptide Fragments; D011347:Procollagen; C042813:procollagen type I carboxy terminal peptide; D015675:Osteocalcin; D012969:Sodium Fluoride; D003094:Collagen; D004247:DNA; D000469:Alkaline Phosphatase; D002117:Calcitriol