title: Cytogenetic analysis of a benzpyrene induced osteosarcoma in the rat (Rattus norvegicus).
abstract: A cytogenetic comparison of primary and transplant tumor cell-lines, both originating from a benzpyrene induced osteosarcoma, with normal rat cell-lines (Rattus norvegicus) is presented here. In all tumor cell-lines tested, the number of chromosomes was increased by one or two. Using Giemsa-banding, structural chromosomal changes, i.e. a Robertsonian translocation t(4;4)(q10;q10) and an interstitial deletion del(6)(q11q16) could be recorded. Furthermore, staining of nucleolus organizer regions (NORs) revealed a shift in NOR activity from chromosome number 11 to 12 and a decrease in NOR activity at chromosome number 3.
authors.affiliation: Institute of Pathological Anatomy, Martin Luther University Halle Wittenberg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: F W
authors.initials: FW
authors.lastname: Rath
mesh_terms: D000782:Aneuploidy; D000818:Animals; D001564:Benzo(a)pyrene; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D007621:Karyotyping; D009368:Neoplasm Transplantation; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D012516:Osteosarcoma / Q000139:chemically induced / Q000235:genetics*; D051381:Rats; D016622:Silver Staining; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D001564:Benzo(a)pyrene