Expression of 11 beta-hydroxysteroid dehydrogenase mRNA in rat vascular smooth muscle cells.
The enzyme 11 beta-hydroxysteroid dehydrogenase (11-HSD) converts corticosterone to the inactive 11-dehydrocorticosterone in the rat. The bioactivity of 11-HSD, expressed as the percentage conversion of 3H-corticosterone to 3H-11-dehydrocorticosterone, was 13.7 +/- 0.4% in rat vascular smooth muscle cells (rVSMC). Cells treated with 100 nM dexamethasone (Dex) showed a 1.4-fold (p < 0.05) increase in 11-HSD activity. Insulin (100 microM) decreased 11-HSD activity by 0.8-fold (p < 0.05). Expression of 11-HSD mRNA was also confirmed in rVSMC by Northern blot analysis. Dexamethasone increased and insulin decreased the levels of 11-HSD mRNA in parallel with the increase in bioactivity. Vascular smooth muscle cells express 11-HSD activity; the access of corticosterone to vascular smooth muscle receptors may be modulated by the enzyme.
Takeda
Y
Y
Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.
Miyamori
I
I
Yoneda
T
T
Ito
Y
Y
Takeda
R
R
D042842:11-beta-Hydroxysteroid Dehydrogenases; D000818:Animals; D002478:Cells, Cultured; D003345:Corticosterone / Q000031:analogs & derivatives / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics*; D006913:Hydroxysteroid Dehydrogenases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D013268:Stimulation, Chemical; D014316:Tritium
D007328:Insulin; D012333:RNA, Messenger; D014316:Tritium; D003907:Dexamethasone; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; C003552:11-dehydrocorticosterone; D003345:Corticosterone