title: Oxygen radical induced mutagenesis is DNA polymerase specific.
abstract: Oxygen free radicals are produced in large amounts by normal cellular processes. Damage to DNA by these reactive species has been implicated in mutagenesis and may be important in the etiology of a variety of human diseases. In this study we investigate the types of mutations produced in vitro as a result of DNA damage by oxygen free radicals. We used a lacZ alpha forward mutation assay in which M13 viral DNA is damaged in vitro, replicated with purified DNA polymerase alpha or beta, transfected into E. coli, and screened for mutations by reduced alpha-complementation of beta-galactosidase activity. By determining the effects of damaged templates on the fidelity of individual DNA polymerases involved in replication and repair, we address the role of specific DNA polymerases in mutagenesis induced by reactive oxygen species. Aerobic incubation of DNA with 100 microM CuCl, 10 microM H2O2 and 100 microM ascorbic acid results in a 3.3-fold and a 3.6-fold elevation in mutation frequency for polymerases alpha and beta, respectively. The specificity and location of the induced mutations, however, are entirely different. For polymerase alpha, A to C, and C to A transversions and deletions of C are each elevated more than 10-fold over their frequencies on undamaged template. For polymerase beta, A to T, C to T, C to A, G to C, and G to T substitutions, and deletions of G are elevated by damage. The frequency of mutants containing two or more closely spaced substitutions is also markedly increased by template damage although the types of mutations and their positions are again specific to each DNA polymerase. We conclude that, for oxidative lesions, the frequency and the types of mutations are determined in part by the DNA polymerase that encounters the site of damage.
authors.affiliation: Joseph Gottstein Memorial Cancer Research Laboratory, Department of Biochemistry, University of Washington, Seattle 98195.
authors.forename: D I
authors.initials: DI
authors.lastname: Feig
authors.forename: L A
authors.initials: LA
authors.lastname: Loeb
mesh_terms: D001205:Ascorbic Acid / Q000633:toxicity; D017104:Bacteriophage M13 / Q000378:metabolism; D001483:Base Sequence; D004249:DNA Damage; D004256:DNA Polymerase I / Q000378:metabolism*; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli; D005609:Free Radicals / Q000633:toxicity; D005816:Genetic Complementation Test; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010100:Oxygen / Q000633:toxicity*; D013698:Templates, Genetic; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D005609:Free Radicals; D006861:Hydrogen Peroxide; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D001616:beta-Galactosidase; D001205:Ascorbic Acid; D010100:Oxygen