The determination of delta-5-androstenediol and its sulphate in serum and urine by gas chromatography-mass spectrometry.
OBJECTIVE
We developed an assay for delta-5-androstenediol (adiol) and delta-5-androstenediol-3-sulphate (adiol-3S) in serum and adiol and total adiol sulphate (adiol-S) in urine.


METHODS
An analytical procedure using HPLC and gas chromatography-mass spectrometry was devised and tested for its reliability.


METHODS
After addition of deuterated androstenediol as internal standard, serum and urine samples were extracted. Steroid sulphates were hydrolysed. The extracts and hydrolysates were purified on HPLC, adiol was derivatized using heptafluorobutyric anhydride and finally quantified by gas chromatography-mass spectrometry.


RESULTS
The assay is accurate and reproducible. The coefficient of variation (CV) for the determination of adiol in serum samples is 4% (intra-assay) and 9% (interassay) and for urine samples 3 and 8% respectively. The intra-assay CV for the adiol-3S analyses is 5% for serum and 2% for urine samples while the interassay CV values for adiol-3S are 10% for serum and 7% for urine samples. The recovery of adiol and adiol-3S from serum and urine samples is 97%.


CONCLUSIONS
The developed assay meets the analytical demands needed for clinical applications.
Dikkeschei
L D
LD
Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
Wolthers
B G
BG
Willemse
P H
PH
van der Pol
H
H
de Ruyter-Buitenhuis
A W
AW
Nagel
G T
GT
D015114:Androstenediol / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D015203:Reproducibility of Results
C033537:5-androstene-3 beta,17 beta-diol sulfate; D015114:Androstenediol