[The effect of endotoxin on the neutrophil chemiluminescence in the rabbit].
To examine the effects of endotoxin on the neutrophil function, neutrophil-dependent microbicidal activities were evaluated by the chemiluminescence (CL) method in rabbits injected with endotoxin. Six rabbits in the first group were administered saline intravenously as control. Ten rabbits each in the second and third group received endotoxin 0.7 microgram.kg-1 and 70 micrograms.kg-1 respectively. Luminol-dependent CL in whole blood (whole blood CL) and in isolated neutrophils (neutrophil CL) were examined before administration and 30, 60, 180 and 360 min after administration. Peak values of whole blood CL in the second group were significantly depressed at 30 and 60 min, and elevated at 360 min. Those in the third group were depressed more than in the second group at all times after endotoxin administration. Peak values of neutrophil CL in the second group at 180 and 360 min were significantly elevated, but those in the third group at 30, 60, 180 min were markedly depressed. Experiments combining neutrophils and pooled plasma revealed that these CL responses were associated with the neutrophils themselves. It is speculated that the neutrophil suppression observed in the third group was caused by the overactivation of neutrophils induced by the activated complements and some other stimulating factors, and/or may be due to the abundant appearance of immature granulocytes with lesser activities.
Asao
Y
Y
Department of Anesthesiology and Resuscitology, Okayama University Medical School.
D000818:Animals; D004731:Endotoxins / Q000494:pharmacology*; D007958:Leukocyte Count / Q000187:drug effects; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011817:Rabbits; D012772:Shock, Septic / Q000097:blood
D004731:Endotoxins