Characterization of a Marek's disease virus BamHI-L-specific cDNA clone obtained from a Marek's disease lymphoblastoid cell line.
Two Marek's disease (MD) virus BamHI-L-specific cDNA clones were isolated from a cDNA library constructed from poly(A)+ RNA fractions of an MD lymphoblastoid cell line, MDCC-CU41 (CU41). These clones were mapped to the region corresponding to the BamHI-Q2 and L-regions. These clones hybridized with 2.5-, 0.8-, and 0.6-kb transcripts prepared from CU41. The transcriptional unit of the 0.6-kb transcript was determined by RNase protection assays. An open reading frame encoding a 107-amino-acid polypeptide was identified in the 0.6-kb transcript. Reverse transcriptase-PCR demonstrated the presence of this transcript in both CU41 and a reticuloendotheliosis virus-transformed cell line latently infected with MD virus.
Ohashi
K
K
Department of Avian and Aquatic Animal Medicine, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
Zhou
W
W
O'Connell
P H
PH
Schat
K A
KA
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D006412:Hematopoietic Stem Cells / Q000382:microbiology; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D008214:Lymphocytes / Q000382:microbiology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D012367:RNA, Viral; D015245:Deoxyribonuclease BamHI