Human leukocytes contain angiotensin I, angiotensin II and angiotensin metabolites.
Angiotensin I (ANG I) and angiotensin II (ANG II) were measured radioimmunologically in human leukocytes extracted with a mixture of acetone, 1N HCl and water (40:1:10 vol). The analytical recoveries of 125I-ANG I and 125I-ANG II, which were added prior to extraction, were 92.00 +/- 3.10 and 99.19 +/- 0.91% (mean +/- SEM; n = 12). The concentration of ANG I- and ANG II-like material in leukocytes from healthy volunteers was 32.04 +/- 3.64 and 13.05 +/- 1.26 fmol/mg protein (n = 24). The immunoreactive material could be characterized on HPLC as Ile5-ANG I, Ile5-ANG II and angiotensin metabolites such as Ile4-ANG III, Ile3-ANG II hexapeptide, Ile2-ANG II pentapeptide and Ile1-ANG II tetrapeptide. From the ANG I-immunoreactive material determined in the extracts, 26% could be characterized on HPLC as Ile5-ANG I. The ANG II-immunoreactive material present in the extracts could be separated on HPLC into 44% Ile5-ANG II, 14% Ile4-ANG III, 7% Ile3-ANG II hexapeptide, 4% Ile2-ANG II pentapeptide and 8% Ile1-ANG II tetrapeptide. Patients with a history of anaphylactic reactions to bee or wasp venom showed significantly reduced ANG II concentrations in their leukocytes as compared to controls (6.76 +/- 0.76 fmol ANG II/mg protein, n = 22, vs. 13.05 +/- 1.26 fmol ANG II/mg protein, n = 24; p < 0.05). A significant correlation between the severity of clinical symptoms and the ANG II levels was found for grade I and II reactions (p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Hermann
K
K
Department of Dermatology, University of Hamburg, FRG.
Ring
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000707:Anaphylaxis / Q000378:metabolism; D000803:Angiotensin I / Q000032:analysis*; D000804:Angiotensin II / Q000032:analysis*; D000818:Animals; D001180:Arthropod Venoms / Q000009:adverse effects; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D006927:Hymenoptera / Q000378:metabolism; D007962:Leukocytes / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay
D001180:Arthropod Venoms; D000804:Angiotensin II; D000803:Angiotensin I