Effect of costimulants on interleukin-2-like activities from goat peripheral blood mononuclear cells.
Culture supernatants containing interleukin-2-like activities (CS-IL2) were prepared from goat peripheral blood cells (mononuclear cells 75% and polymorphonuclear cells 25%). These were stimulated with three costimulants, (tetradecanoyl phorbol acetate, indomethacin and calcium ionophore A23187), either alone or in different combinations, in RPMI-1640 medium (containing 0.5% bovine serum albumin (BSA)) with or without serum. After 18 h of incubation with costimulants, concanavalin A (Con A) was added and the incubation was continued for next 48 h. Higher interleukin-2 (IL-2)-like activities were generated in the culture supernatants prepared in RPMI-1640 growth medium containing 0.5% BSA without serum. Further, IL-2-like activities were much higher in culture supernatants obtained by stimulation with all the three costimulants, as well as Con A, than the two costimulants with Con A or any of the costimulants with Con A.
Tomar
A
A
Immunology Section, Indian Veterinary Research Institute, Izatnagar, UP.
Bansal
M P
MP
Ram
G C
GC
D000818:Animals; D000001:Calcimycin / Q000276:immunology*; D002478:Cells, Cultured; D003470:Culture Media; D004338:Drug Combinations; D005260:Female; D006041:Goats / Q000276:immunology*; D007213:Indomethacin / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate
D003470:Culture Media; D004338:Drug Combinations; D007376:Interleukin-2; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin