[Antimutagenic activity and metabolic transformation of ranunculin by rat liver microsomes].
Ranunculin (RAN) was shown to be an antimutagenic agent selectively against mitomycin C (MMC) or methyl methane sulfanate (MMS) treated Salmonella typhimminum TA100/TA102. It decreased the formation of micronucleus of MMC induced polychromatic erythrocytes (PEC) from 46 +/- 9.2% to 20 +/- 6% in mice. The inhibition of RAN on the incorporation of 3H-TdR into DNA disappeared after incubation with rat liver microsomes and cytoplasm since RAN was found to be metabolized by rat liver microsomes in vitro, resulting in a new absorbance peak at 258 nm, determined by RP-HPLC. The data show that RAN may have both antimutagen and antitumor activity, but the latter action may disappear by metabolic transformation.
Li
R Z
RZ
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Pei
H P
HP
Ji
X J
XJ
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005663:Furans / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D008759:Methylglycosides / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D051381:Rats
D016587:Antimutagenic Agents; D005663:Furans; D008759:Methylglycosides; D004247:DNA; C039859:ranunculin