title: Preservation time dependent morphological changes in cold stored human donor pancreas.
abstract: Pancreatic transplantation is being used to treat insulin-dependent diabetes. An intact structure of the graft is a prerequisite for preserved function and we therefore monitored the light microscopic and ultrastructural changes in 30 human donor pancreases stored in the cold in University of Wisconsin preservation solution. Twenty-three pancreases were stored for less than 24 h and 7 for more than 30 h. All glands stored longer than 30 h displayed cytoplasmic vacuolisation in a variable proportion of acinar cells. In addition, the glands stored over 40 h showed focal acinar necrosis. Endocrine tissue was only slightly affected, while duct cells showed no changes. It is concluded that cold preserved pancreases stored for less than 24 h are best for transplantation purposes and that acinar cells are more sensitive to ischaemic damage than endocrine and duct cells.
authors.affiliation: Department of Pathology, Free University of Brussels VUB, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: In't Veld
authors.forename: H
authors.initials: H
authors.lastname: Nizze
authors.forename: G
authors.initials: G
authors.lastname: Klöppel
mesh_terms: D000241:Adenosine; D000293:Adolescent; D000328:Adult; D000493:Allopurinol; D002648:Child; D015925:Cryopreservation; D005260:Female; D005978:Glutathione; D006801:Humans; D007328:Insulin; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D019188:Organ Preservation Solutions; D010179:Pancreas / Q000033:anatomy & histology* / Q000648:ultrastructure; D011887:Raffinose; D013997:Time Factors; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose