title: Uninterrupted translation through putative 12-nucleotide coding gap in sequence of carA: business as usual.
abstract: Previous work of others reported an untranslated stretch of 12 nucleotides in the 5' coding sequence of carA from Pseudomonas aeruginosa. However, N-terminal protein sequencing of carA-lacZ translational fusions shows that these 12 nucleotides are normally translated in a continuous triplet manner, both in P. aeruginosa and in Escherichia coli.
authors.affiliation: Department of Human Genetics, Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112-1107.
authors.forename: T M
authors.initials: TM
authors.lastname: Tuohy
authors.forename: T
authors.initials: T
authors.lastname: Kidd
authors.forename: R F
authors.initials: RF
authors.lastname: Gesteland
authors.forename: J F
authors.initials: JF
authors.lastname: Atkins
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D008025:Ligases / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
chemical_list: D008025:Ligases