ANF binding sites in the heart of the quail (Coturnix coturnix japonica).
The distribution pattern of rat [125I]-atrial natriuretic factor (ANF) binding sites in the cardiac regions of the Japanese quail was examined by in vitro quantitative autoradiography. Elevated ANF binding densities (519 +/- 121 fmol/mg protein) were found in the posterior vena cava, while lower binding levels (between 40 and 50 fmol/mg protein) were found in sinus venosus, aortic bulb, and endomural vessels, with the ventricular wall having the lowest value (17.6 +/- 8.8 fmol/mg protein). Scatchard analyses of the ANF binding characteristics (Kd, Bmax) revealed both low (94 +/- 55 fmol/mg protein) and high (1161 +/- 69 fmol/mg protein) Bmax values. Receptors with higher Kd values than those observed in other cardiac regions (Kd between 30 and 60 pM) were found in the vena cava and in the heart ventricle (Kd between 113.2 and 229 pM).
Cerra
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MC
Department of Cellular Biology, University of Calabria, Italia.
Canonaco
M
M
Tota
B
B
D000818:Animals; D001345:Autoradiography; D003370:Coturnix / Q000378:metabolism*; D007457:Iodine Radioisotopes; D009206:Myocardium / Q000737:chemistry*; D017461:Receptors, Atrial Natriuretic Factor / Q000032:analysis*
D007457:Iodine Radioisotopes; D017461:Receptors, Atrial Natriuretic Factor