Green fluorescent protein as a marker for gene expression.
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.
Chalfie
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M
Department of Biological Sciences, Columbia University, New York, NY 10027.
Tu
Y
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Euskirchen
G
G
Ward
W W
WW
Prasher
D C
DC
D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D002455:Cell Division; D002469:Cell Separation; D004926:Escherichia coli / Q000235:genetics*; D005453:Fluorescence; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D013050:Spectrometry, Fluorescence; D014170:Transformation, Genetic
D008164:Luminescent Proteins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins