Non-nuclear damage and cell lysis are induced by UVA, but not UVB or UVC, radiation in three strains of L5178Y cells.
The potential to induce non-nuclear changes in mammalian cells has been examined for (1) UVA1 radiation (340-400 nm, UVASUN 2000 lamp), (2) UVA+UVB (peak at 313 nm) radiation (FS20 lamp), and (3) UVC (254 nm) radiation (G15T8 lamp). The effects of irradiation were monitored in vitro using three strains of L5178Y (LY) mouse lymphoma cells that markedly differ in sensitivity to UV radiation. Comparisons were made for the effects of approximately equitoxic fluences that reduced cell survival to 1-15%. Depending on the cell strain, the fluences ranged from 830 to 1600 kJ/m2 for the UVASUN lamp, 75 to 390 J/m2 for the FS20 lamp and 3.8 to 17.2 J/m2 for the G15T8 lamp. At the exposure level used in this study, irradiation with the UVASUN, but not the FS20 or G15T8, lamp induced a variety of non-nuclear changes including damage to cytoplasmic organelles and increased plasma membrane permeability and cell lysis. Cell lysis and membrane permeabilization were induced by the UVA1 emission of the UVASUN lamp, but not by its visible+IR components (> 400 nm). The results show that the plasma membrane and other organelles of LY cells are highly sensitive to UVA1 but not to UVB or UVC radiation. Also UVA1, but not UVB or UVC radiation, causes rapid and extensive lysis of LY cells. In conclusion, non-nuclear damage contributes substantially to UVA cytotoxicity in all three strains of LY cells.
Beer
J Z
JZ
Center for Devices and Radiological Health, Food and Drug Administration, Rockville, MD 20857.
Olvey
K M
KM
Miller
S A
SA
Thomas
D P
DP
Godar
D E
DE
D000818:Animals; D002462:Cell Membrane / Q000528:radiation effects*; D002470:Cell Survival; D004307:Dose-Response Relationship, Radiation; D007425:Intracellular Membranes / Q000528:radiation effects*; D007940:Leukemia L5178 / Q000473:pathology / Q000532:radiotherapy*; D008565:Membrane Proteins / Q000528:radiation effects; D051379:Mice; D011836:Radiation Tolerance; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D008565:Membrane Proteins