title: Construction and characterization of a YAC library with a low frequency of chimeric clones from flow-sorted human chromosome 9.
abstract: Human chromosome 9 DNA, flow-sorted from somatic cell hybrid PK-87-9, has been used to construct two complete digest YAC libraries. The combined representation of chromosome 9 in these libraries, estimated by hybridization of chromosome 9-specific sequences to YAC colony grids, is approximately 95%. The frequency of chimeric clones, analyzed by fluorescence in situ hybridization of chromosome 9 YACs to human metaphase chromosomes, was estimated to be approximately 4%. These libraries provide a resource for physical mapping and for moving from genetic markers to disease loci on chromosome 9.
authors.affiliation: Center for Human Genome Studies and Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
authors.forename: M K
authors.initials: MK
authors.lastname: McCormick
authors.forename: A
authors.initials: A
authors.lastname: Buckler
authors.forename: W
authors.initials: W
authors.lastname: Bruno
authors.forename: E
authors.initials: E
authors.lastname: Campbell
authors.forename: K
authors.initials: K
authors.lastname: Shera
authors.forename: D
authors.initials: D
authors.lastname: Torney
authors.forename: L
authors.initials: L
authors.lastname: Deaven
authors.forename: R
authors.initials: R
authors.lastname: Moyzis
mesh_terms: D002678:Chimera; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005091:Exons; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D015183:Restriction Mapping
chemical_list: D004247:DNA