Antioxidant status and dietary lipid unsaturation modulate oxidative DNA damage.
High performance liquid chromatography with electrochemical detection (HPLC-EC) was used to measure 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker for oxidative DNA damage, in mammary gland isolated from tumor-bearing and tumor-free rats fed diets of varied fatty acid composition and vitamin E and selenium content. A method for tissue preparation and analysis is reported and a significant positive correlation shown between degree of unsaturation of dietary fatty acids and 8-OHdG concentration, regardless of antioxidant status. The increase in 8-OHdG concentration with greater fatty acid unsaturation was more pronounced in the absence of adequate dietary vitamin E and selenium. The implications of these data for defining the role of dietary lipid in the process of mammary carcinogenesis are discussed.
Haegele
A D
AD
AMC Cancer Research Center, Denver, CO.
Briggs
S P
SP
Thompson
H J
HJ
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005260:Female; D015227:Lipid Peroxidation; D008321:Mammary Glands, Animal / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D004273:DNA, Neoplasm; D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine