Antisense RNA inactivation of gp138 gene expression results in repression of sexual cell fusion in Dictyostelium discoideum.
A glycoprotein, gp138, is implicated in the sexual cell fusion of Dictyostelium discoideum. We previously cloned and sequenced the two genes encoding the gp138 protein, GP138A and GP138B (Fang et al. (1993) Dev. Biol. 156, 201-208). Here, we have constructed a vector producing antisense RNA for the gp138 genes and have transformed the vector into Dictyostelium cells. The transformed cells showed a reduction in the amounts of gp138 mRNA and protein and their sexual cell fusion activity was considerably repressed.
Fang
H
H
Institute of Biological Sciences, University of Tsukuba, Japan.
Aiba
K
K
Higa
M
M
Urushihara
H
H
Yanagisawa
K
K
D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000502:physiology; D004023:Dictyostelium / Q000235:genetics* / Q000502:physiology; D005306:Fertilization / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D016372:RNA, Antisense; D014170:Transformation, Genetic
D015815:Cell Adhesion Molecules; D016372:RNA, Antisense; C080221:glycoprotein 138, Dictyostelium