Analysis of the influence of chromosomal condensation on the activity of restriction endonucleases.
The different responses of metaphase chromosomes to restriction endonuclease activity depend on the stage of chromosomal condensation. Results obtained established that this factor plays a remarkable role in obtaining high resolution banding patterns which facilitated visualization of a large number of sub-bands. It is evident that various restriction endonucleases, which are able to induce a coincident G-like banding pattern on Don cell line chromosomes, act in very different ways.
González-Tizón
A M
AM
Departamento de Biología Celular y Molecular, Area de Genética, Universidad de la Coruña, Spain.
Martínez-Lage
A
A
Méndez
J
J
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002875:Chromosomes / Q000378:metabolism* / Q000648:ultrastructure; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D015247:Deoxyribonuclease HindIII / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008677:Metaphase
D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases