Further characterization of an interleukin-2-like cytokine produced by Xenopus laevis T lymphocytes.
A T-cell growth factor (TCGF) is produced by antigen- or mitogen-stimulated T lymphocytes from the South African clawed frog Xenopus laevis. This study further defines the physical and biological properties of this cytokine and demonstrates that TCGF is biochemically similar to mammalian interleukin-2 (IL-2). Biologically active TCGF eluted from SDS-PAGE displays a M(r) of 16 kD and lectin-affinity chromatography indicates that the three-dimensional configuration of carbohydrates on TCGF and human IL-2 is similar. Secretion of TCGF is detectable 1 day after stimulation of splenocytes with the T-cell mitogen phytohemagglutinin (PHA) and peaks following 2 to 3 days of stimulation. Finally, despite the biological and physical similarities between Xenopus TCGF and mammalian IL-2, anti-human IL-2 monoclonal antibodies do not recognize Xenopus TCGF.
Haynes
L
L
Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642.
Cohen
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology; D008213:Lymphocyte Activation; D011233:Precipitin Tests; D014415:Tunicamycin; D014982:Xenopus laevis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D014415:Tunicamycin