Evidence for age-dependent changes in motoneuron dendritic morphology following neonatal nerve-crush in the rat.
Motoneurons supplying the extensor hallucis longus muscle of the rat were temporarily separated from the muscle by sciatic nerve-crush at five days postnatally. Such treatment permanently alters the reflex response of the affected motoneurons without the large-scale cell death associated with nerve-crush at birth. After reinnervation, the motoneurons were retrogradely labelled with cholera toxin subunit-B conjugated to horseradish peroxidase and the dendritic tree of each labelled cell was analysed. When compared to normal data, significantly higher levels of dendritic density were observed in the rostrodorsally orientated parts of the dendritic field. This was similar to that found previously for the same motor pool after nerve-crush at birth. However, in other parts of the field where a lower dendritic density was found after nerve-crush at birth, no change was seen after nerve-crush at five days. These data present evidence for the influence of sensory afferents on the development of motoneuron dendrites. Taken together with the previous findings after nerve-crush at birth, we suggest that the differential dendritic changes caused by neonatal nerve lesion contribute to an imbalance in the pattern of excitatory and inhibitory inputs to the motoneuron, which results either in cell death, or the abnormal activity seen in those motoneurons which survive.
O'Hanlon
G M
GM
Department of Anatomy and Cell Biology, Saint Mary's Hospital Medical School, Imperial College, London, U.K.
Lowrie
M B
MB
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002772:Cholera Toxin; D003712:Dendrites / Q000648:ultrastructure*; D006735:Horseradish Peroxidase; D009046:Motor Neurons / Q000648:ultrastructure*; D009132:Muscles / Q000294:innervation; D009409:Nerve Crush; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000502:physiology*; D014034:Toes
D002772:Cholera Toxin; D006735:Horseradish Peroxidase