Kinetic fluorimetric determination of promethazine by a stopped-flow mixing technique.
The oxidation of promethazine to its corresponding fluorescent sulphoxide was used to develop a novel kinetic fluorimetric method for the determination of this drug. The use of a stopped-flow mixing technique makes use of an oxidizing reagent unnecessary because the oxidation is rapidly carried out by dissolved oxygen. The method is simple and fast as it only requires a few seconds to obtain kinetic data which allows ready application to routine analyses. The calibration graph is linear over the range 0.5-80 micrograms ml-1 and the precision (%RSD) is close to 2%. The method was applied to the determination of promethazine hydrochloride in two pharmaceutical preparations.
de la Peña
L
L
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Gómez-Hens
A
A
Pérez-Bendito
D
D
D002626:Chemistry, Pharmaceutical / Q000379:methods; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D011398:Promethazine / Q000032:analysis*; D012996:Solutions / Q000737:chemistry; D013607:Tablets / Q000737:chemistry
D012996:Solutions; D013607:Tablets; D011398:Promethazine