Construction of a shuttle vector for use between Pasteurella multocida and Escherichia coli.
The isolation of a small plasmid from Pasteurella multocida has enabled the construction of a shuttle vector for use between P. multocida and Escherichia coli. The vector pBAC64 contains the origin of replication from P. multocida, an antibiotic resistance gene which functions in P. multocida, and the E. coli vector pUC18. The presence of the pUC18 multiple cloning site together with the lacZ' gene provides a screening method and allows cloning and manipulation in E. coli as well as cloning in P. multocida.
Bills
M M
MM
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
Medd
J M
JM
Chappel
R J
RJ
Adler
B
B
D003001:Cloning, Molecular / Q000379:methods*; D004261:DNA Replication; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D016979:Pasteurella multocida / Q000235:genetics*; D010957:Plasmids / Q000302:isolation & purification; D015183:Restriction Mapping; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D001616:beta-Galactosidase