Osmometric behavior, hydraulic conductivity, and incidence of intracellular ice formation in bovine oocytes at different developmental stages.
Bovine oocytes that were immature (IMM), matured in vitro (IVM) or in vivo (MAT), or matured and fertilized in vitro (IVF) were studied using a microscope diffusion chamber to estimate osmotic parameters and a cryomicroscope to characterize intracellular ice formation (IIF). Linear Boyle van't Hoff relationships were observed with all four types of oocytes between 0.265 and 0.799 osm NaCl. At 20 degrees C, estimates of hydraulic conductivity (Lp) were significantly higher for IVM oocytes than IMM and MAT oocytes (0.84 micron/(min.atm) vs 0.45 and 0.47, respectively). IVM oocytes also tended to have higher Lp values than IVF oocytes (0.55 micron/(min.atm)). At 5 degrees C, the Lp of IVM oocytes decreased to 0.36 micron/min.atm) corresponding to an Arrhenius activation energy of 7.84 kcal/mol. The incidence of IIF in MAT oocytes suspended in salt solution and subjected to linear cooling to -60 degrees C was 45% at 4 degrees C/min, 75% at 8 degrees C/min, and 93% at 16 degrees C/min; with IVF oocytes, the incidence of IIF was 40% at 4 degrees C/min, 92% at 8 degrees C/min, and 100% at 16 degrees C/min. Comparisons involving median IIF temperatures (TIIF50s) and the distributions of the observed IIF temperatures for IMM (Myers et al., Cryo-Lett. 8, 260), IVM (Chandrasekaran et al., Cryobiology 27, 676), MAT and IVF oocytes indicated that the IIF incidence in IMM oocytes cooled at 4 degrees C/min was greater than that of oocytes at the other developmental stages cooled at the same rate. The TIIF50s of IVM and IVF oocytes were lowered by equilibration in 1.5 M ethylene glycol (EG), glycerol, or propylene glycol (PG) prior to cooling, with EG tending to lower the TIIF50s more than glycerol or PG. For all three cryoprotectants, the TIIF50s and IFF temperature distributions were cooling-rate dependent. The Weibull probability distribution was fitted to the distributions of the IIF temperatures of oocytes suspended in salt solutions with and without cryoprotectants yielding R2 values ranging from 0.70 to 0.98.
Ruffing
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NA
Department of Animal Science, Cornell University, Ithaca, New York 14853-5701.
Steponkus
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PL
Pitt
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RE
Parks
J E
JE
D000818:Animals; D002417:Cattle; D048429:Cell Size; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D019855:Ethylene Glycol; D005026:Ethylene Glycols; D005260:Female; D005307:Fertilization in Vitro; D005990:Glycerol; D007053:Ice; D066298:In Vitro Techniques; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D009995:Osmosis; D019946:Propylene Glycol; D011409:Propylene Glycols; D013816:Thermodynamics; D015053:Zygote / Q000378:metabolism
D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D007053:Ice; D011409:Propylene Glycols; D019946:Propylene Glycol; D019855:Ethylene Glycol; D005990:Glycerol