title: Modification of liposomes by addition of HCO60. II. Encapsulation of doxorubicin into liposomes containing HCO60.
abstract: The efficiency of encapsulation of doxorubicin (DOX) into liposomes containing HCO60 was studied. Empty egg phosphatidylcholine (PC) liposomes containing various amounts of HCO60 were passed through a polycarbonate membrane filter (0.1 micron). Closed vesicles were formed even with liposomes containing 90 wt% HCO60. DOX was encapsulated efficiently (more than 1 mg DOX/8 mg lipids) using a pH-gradient method. HCO60 accelerated the leakage of DOX from liposomes into phosphate buffer at temperatures above 25 degrees C. HCO60 suppressed the leakage of DOX from egg PC/cholesterol (7/3, w/w) liposomes into phosphate buffer containing 50% fetal bovine serum (FBS). Studies on the interaction between liposomes and serum components using zeta potential and turbidity suggest that HCO60 prevents the liposomes from interacting with serum components.
authors.affiliation: Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Shizuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Hosokawa
authors.forename: Y
authors.initials: Y
authors.lastname: Okubo
authors.forename: E
authors.initials: E
authors.lastname: Hayakawa
authors.forename: K
authors.initials: K
authors.lastname: Ito
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D002368:Castor Oil / Q000031:analogs & derivatives*; D002417:Cattle; D004317:Doxorubicin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004337:Drug Carriers; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000008:administration & dosage* / Q000737:chemistry; D012709:Serum Albumin / Q000494:pharmacology; D013501:Surface-Active Agents
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D012709:Serum Albumin; D013501:Surface-Active Agents; C023040:polyethoxylated castor oil; D002368:Castor Oil; D004317:Doxorubicin