Isolation of novel non-HLA gene fragments from the hemochromatosis region (6p21.3) by cDNA hybridization selection.
It has previously been shown that cDNA hybridization selection can identify and recover novel genes from large cloned genomic DNA such as cosmids or YACs. In an effort to identify candidate genes for hemochromatosis, this technique was applied to a 320-kb YAC containing the HLA-A gene. A short fragment cDNA library derived from human duodenum was selected with the YAC DNA. Ten novel gene fragments were isolated, characterized, and localized on the physical map of the YAC.
Goei
V L
VL
Department of Pediatrics, Yale University School of Medicine, New Haven, CT 06510.
Parimoo
S
S
Capossela
A
A
Chu
T W
TW
Gruen
J R
JR
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D018244:Chromosomes, Artificial, Yeast; D002896:Chromosomes, Human, Pair 6; D018076:DNA, Complementary; D015234:HLA-A Antigens / Q000235:genetics*; D006432:Hemochromatosis / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D017421:Sequence Analysis
D018076:DNA, Complementary; D015234:HLA-A Antigens