title: Carboxyl methylation of protein phosphatase 2A from Xenopus eggs is stimulated by cAMP and inhibited by okadaic acid.
abstract: Protein phosphatase 2A is the principal carboxyl methylated protein in cytoplasmic extracts derived from Xenopus eggs arrested in interphase. Levels of methylation approach stoichiometric values after 2 hr of incubation at 22 degrees C. Rates of PP2A methylation increase by over 50% upon addition of cAMP. Methylation is inhibited by okadaic acid at concentrations that inhibit PP2A phosphatase activity.
authors.affiliation: Department of Molecular Biology, Princeton University, NJ 08544.
authors.forename: M
authors.initials: M
authors.lastname: Floer
authors.forename: J
authors.initials: J
authors.lastname: Stock
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D000242:Cyclic AMP / Q000494:pharmacology*; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004988:Ethers, Cyclic / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008745:Methylation; D019319:Okadaic Acid; D010063:Ovum / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D054648:Protein Phosphatase 2; D012436:S-Adenosylmethionine / Q000378:metabolism*; D015290:Second Messenger Systems; D013379:Substrate Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014982:Xenopus laevis
chemical_list: D002147:Calmodulin; D002364:Caseins; D004988:Ethers, Cyclic; D019319:Okadaic Acid; D012436:S-Adenosylmethionine; D000242:Cyclic AMP; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium