Purification and characterisation of the restriction endonuclease ItaI from Ilyobacter tartaricus recognizing 5'-GC decreases NGC-3'.
ItaI, an isoschizomer of the subclass IIW [Kessler and Manta, Gene 92 (1990) 1-248] restriction endonuclease (ENase), Fnu4HI [Leung et al., Nucleic Acids Res. 6 (1979) 17-25], has been isolated from Ilyobacter tartaricus. The ENase has the five-base palindromic recognition sequence, 5'GC decreases NGC-3'. It cleaves behind the second nucleotide and produces a one-nt 5' overhang.
Städtler
P
P
Department of Microbiology, Ruhr-Universität Bochum, Germany.
von Strandmann
R P
RP
Walter
T
T
Frey
B
B
Auer
H
H
Hengstenberg
W
W
Schmitz
G
G
D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology*; D012091:Repetitive Sequences, Nucleic Acid; D013379:Substrate Specificity
C069758:endodeoxyribonuclease Fnu4HI; D015252:Deoxyribonucleases, Type II Site-Specific