[Cryptosporidium parvum in patients with chronic diarrhea and AIDS: diagnosis by means of indirect immunofluorescence with monoclonal antibodies].
The diagnosis of cryptosporidiosis is difficult when oocyst elimination is poor as occurs in AIDS patients. Aiming to improve the diagnosis, 144 fecal samples coming from AIDS patients with diarrhea, were studied using indirect immunofluorescence with anti-Cryptosporidium monoclonal antibodies. The results were compared with Ziehl Neelsen and safranine stainings. Twenty three samples (15.9%) were positive for Cryptosporidium with at least one of the three methods. Sensitivities were 78.3% for immunofluorescence, 86.9% for Ziehl Neelsen and 91.3 for safranine stainings. The specificity of the three methods was 100%. It is concluded that immunofluorescence does not improve the diagnostic accuracy of cryptosporidiosis and its high cost precludes its use in routine laboratories.
Weitz
J C
JC
Laboratorio de Referencia de Parasitología, Instituto de Salud Pública, Santiago de Chile.
Astorga
B
B
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis*; D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000032:analysis*; D002908:Chronic Disease; D003457:Cryptosporidiosis / Q000175:diagnosis*; D016785:Cryptosporidium parvum / Q000276:immunology*; D003967:Diarrhea / Q000175:diagnosis*; D004311:Double-Blind Method; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D006801:Humans; D011446:Prospective Studies; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan
Cryptosporidium parvum en pacientes con diarrea crónica y SIDA: diagnóstico mediante immunofluorescencia indirecta con anticuerpos monoclonales.