title: Isolation and sequencing of deer and sheep insulin-like growth factors-I and -II.
abstract: We have developed a simple method for the isolation of highly purified cervine (c) and ovine (o) insulin-like growth factors-I (IGF-I) and -II. The IGFs were isolated from acidified serum by cation exchange chromatography and then purified by gel filtration, chromatofocusing, and reverse-phase chromatography. The IGF preparations are > 95% pure. The cIGF-I preparation contains < 0.056% cIGF-II and the oIGF-I preparation contains < 0.01% oIGF-II. Both the IGF-II preparations contain < 0.01% IGF-I. The amino acid sequence of cIGF-I has two differences when compared with human (h) IGF-I. The cIGF-II sequence, which is identical to bovine IGF-II, has three differences when compared with hIGF-II.
authors.affiliation: Wallaceville Animal Research Centre, AgResearch, Upper Hutt, New Zealand.
authors.forename: L G
authors.initials: LG
authors.lastname: Moore
authors.forename: D
authors.initials: D
authors.lastname: Jones
authors.forename: M A
authors.initials: MA
authors.lastname: Lymburn
authors.forename: S C
authors.initials: SC
authors.lastname: Hodgkinson
authors.forename: S R
authors.initials: SR
authors.lastname: Davis
authors.forename: J M
authors.initials: JM
authors.lastname: Suttie
authors.forename: M
authors.initials: M
authors.lastname: Sadighi
authors.forename: A
authors.initials: A
authors.lastname: Carne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003670:Deer / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D011869:Radioligand Assay; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000378:metabolism*
chemical_list: D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II