Pretranslational regulation of the expression of the lipoprotein lipase (EC 3.1.1.34) gene by dietary fatty acids in the rat.
Although there have been a number of studies of effects of diet and hormones on lipoprotein lipase (EC 3.1.1.34; LPL) activity and levels of LPL mRNA (Raynolds et al. 1990), there have been no studies which have investigated effects of different dietary fatty acids on LPL gene expression. In the present study male Wistar Albino rats were pair-fed diets containing 50 g fat/kg of different fatty acid composition for 2 weeks. The diets fed were (1) a mixed oil (450 g saturated fatty acids, 420 g monounsaturated fatty acids, 130 g polyunsaturated fatty acids/kg; n 8), (2) maize oil (n 8), or (3) fish oil (n 8). Animals were killed, RNA was extracted from liver and perirenal and epididymal fat pads, and analysed by 'Northern methodology'. Samples were hybridized to a human cDNA probe for LPL (Gotoda et al. 1989). Two transcripts were identified in epididymal and perirenal adipose tissue which were approximately 3.7 and 1.7 kb in size. The results suggested that (1) fish oil-fed animals had significantly greater production of LPL mRNA in epididymal adipose tissue compared with maize oil-fed animals (P < 0.05), (2) maize oil-fed animals had significantly greater production of LPL mRNA in perirenal fat compared with the other dietary groups (P < 0.05), (3) expression in the liver was not significant. Rats fed on a fish oil diet had significantly reduced plasma triacylglycerol concentrations compared with the mixed-oil group (P < 0.05), but there were no significant differences in plasma cholesterol. The differences in LPL could not be explained directly by the changes in plasma immunoreactive-insulin and glucose-dependent insulinotrophic polypeptide levels in the three groups.
Murphy
M C
MC
Nutritional Metabolism Research Group, School of Biological Sciences, University of Surrey, Guildford.
Zampelas
A
A
Puddicombe
S M
SM
Furlonger
N P
NP
Morgan
L M
LM
Williams
C M
CM
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D015152:Blotting, Northern; D002784:Cholesterol / Q000097:blood; D003314:Corn Oil / Q000008:administration & dosage; D004822:Epididymis; D005227:Fatty Acids / Q000008:administration & dosage*; D005395:Fish Oils / Q000008:administration & dosage; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007668:Kidney; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
D005227:Fatty Acids; D005395:Fish Oils; D012333:RNA, Messenger; D014280:Triglycerides; D003314:Corn Oil; D002784:Cholesterol; D008071:Lipoprotein Lipase