title: Presence of HIV-1 in human parenchymal and non-parenchymal liver cells in vivo.
abstract: The infection of liver cells by HIV-1 was investigated in vivo. Liver biopsies from 13 anti-HIV-1 antibody-positive patients were studied and HIV-1 DNA was revealed by polymerase chain reaction (PCR) in eight. In situ hybridization demonstrated the presence of HIV-1 RNA in all eight PCR-positive liver specimens. Mononuclear inflammatory cells in the portal tracts and Kupffer cells were labeled by a HIV-1 35S-RNA probe in all cases and by an anti-p24 monoclonal antibody, in seven cases. In addition, hepatocytes also clearly scored positive for HIV-1 RNA in three cases. These results demonstrate the infection of both parenchymal and non-parenchymal liver cells by HIV-1 in vivo and therefore show that HIV-1 can infect an epithelial CD4-negative cell type.
authors.affiliation: INSERM U-370, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Housset
authors.forename: E
authors.initials: E
authors.lastname: Lamas
authors.forename: V
authors.initials: V
authors.lastname: Courgnaud
authors.forename: O
authors.initials: O
authors.lastname: Boucher
authors.forename: P M
authors.initials: PM
authors.lastname: Girard
authors.forename: C
authors.initials: C
authors.lastname: Marche
authors.forename: C
authors.initials: C
authors.lastname: Brechot
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology*; D001483:Base Sequence; D001706:Biopsy; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008099:Liver / Q000737:chemistry / Q000382:microbiology* / Q000473:pathology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral