title: Distribution of Ca(2+)-modulating proteins in sarcoplasmic reticulum membranes after denervation.
abstract: The early response to the loss of motor innervation to the muscle is connected with an altered Ca(2+)-homeostasis. Our study, based on Western blotting, indicates that denervation influenced expression of some sarcoplasmic Ca(2+)-modulating proteins. Evidence has been brought for an increase of the level of calsequestrin and of the putative ryanodine receptor paralleled with a slight decrease of the total amount of Ca(2+)-pump protein. The expression of unchanged Ca(2+)-pump isoform and unaltered quantities of other non-junctional Ca(2+)-binding proteins support the hypothesis that changed cellular Ca2+ homeostasis include also an alteration of Ca(2+)-modulating systems, mainly from the junctional region of sarcoplasmic membranes.
authors.affiliation: Comenius University, Jessenius Medical School, Department of Biochemistry, Martin, Slovakia.
authors.forename: J
authors.initials: J
authors.lastname: Lehotský
authors.forename: G
authors.initials: G
authors.lastname: Bezáková
authors.forename: P
authors.initials: P
authors.lastname: Kaplán
authors.forename: L
authors.initials: L
authors.lastname: Raeymaekers
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D002155:Calsequestrin / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006614:Hindlimb / Q000294:innervation; D007527:Isoenzymes / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D009121:Muscle Denervation; D009132:Muscles / Q000294:innervation / Q000378:metabolism*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D012584:Sciatic Nerve / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D002155:Calsequestrin; D007527:Isoenzymes; D000252:Calcium-Transporting ATPases; D002118:Calcium