title: A scanning tunnelling microscopy comparison of passive antibody adsorption and biotinylated antibody linkage to streptavidin on microtiter wells.
abstract: An antiferritin antibody was either, (a) passively adsorbed to microwells or (b) biotinylated and immobilised to streptavidin coated microwells. Scanning tunnelling microscope (STM) imaging of these well surfaces coated with a platinum (95%) carbon (5%) coating (Pt/C) conductive layer showed a randomly oriented array of antibodies for passive adsorption whereas for biotin-streptavidin immobilisation there was a more uniform and even distribution of antibodies on the well surface. On further incubation with ferritin STM imaging showed that for passive adsorption approximately 5% of the surface was functional, while for the biotinylated antibody it was greater than 60%. The images presented in this paper show graphically the loss of functionality that occurs using passive adsorption and, conversely, the preservation of antibody functionality using the biotin-streptavidin linkage for antibody immobilisation. These results correlate well with the work of others in the field.
authors.affiliation: Kodak Clinical Diagnostics Ltd. (KCDL), Pollards Wood Laboratories, Chalfont-St.-Giles, Buckinghamshire, UK.
authors.forename: J
authors.initials: J
authors.lastname: Davies
authors.forename: A C
authors.initials: AC
authors.lastname: Dawkes
authors.forename: A G
authors.initials: AG
authors.lastname: Haymes
authors.forename: C J
authors.initials: CJ
authors.lastname: Roberts
authors.forename: R F
authors.initials: RF
authors.lastname: Sunderland
authors.forename: M J
authors.initials: MJ
authors.lastname: Wilkins
authors.forename: M C
authors.initials: MC
authors.lastname: Davies
authors.forename: S J
authors.initials: SJ
authors.lastname: Tendler
authors.forename: D E
authors.initials: DE
authors.lastname: Jackson
authors.forename: J C
authors.initials: JC
authors.lastname: Edwards
mesh_terms: D000327:Adsorption; D000906:Antibodies; D001426:Bacterial Proteins; D001710:Biotin / Q000031:analogs & derivatives*; D005293:Ferritins / Q000276:immunology; D007118:Immunoassay; D016252:Microscopy, Scanning Tunneling; D010969:Plastics; D019809:Streptavidin; D013499:Surface Properties
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins; D010969:Plastics; D001710:Biotin; D005293:Ferritins; D019809:Streptavidin