c-fos proto-oncogene transient transcription is negatively affected in the ELa4-2 transformed rat cell line.
To study the effects of the regulatory phosphoprotein ICP4 of the Herpes simplex virus, (HSV), a DNA tumor virus, on the induction of gene expression by the epidermal growth factor (EGF), we have constructed a cell line, ELa4-2, which constitutively expresses the a-4 gene product. The ELa4-2 cells are derived from the rat fibroblast EL2, in which EGF induces a marked c-fos and c-myc proto-oncogene transcription. Here we report that in ELa4-2 cells, the gene expression induced by EGF was negatively affected in respect to that obtained stimulating the parental EL2 cells. In particular, we studied the c-fos and c-myc proto-oncogene transcription induced by EGF. We found that in ELa4-2 cells the c-fos induction was dramatically reduced in comparison with the c-fos induction obtained in the parental EL2 cells. On the contrary, the c-myc induction by EGF was not affected by the presence of ICP4. Finally, we compared the HSV infectivity in ELa4-2 versus the EL2 cells. We showed that the virus growth capability was reduced, in the cells expressing ICP4.
Tognon
M
M
Chair of Biology, School of Medicine, University of Ferrara, Italy.
Romanelli
M G
MG
Cattozzo
E M
EM
Bovolenta
C
C
Liboi
E
E
D000818:Animals; D002461:Cell Line, Transformed; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation / Q000235:genetics; D016762:Genes, fos / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000502:physiology; D009687:Nuclear Proteins / Q000494:pharmacology; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015534:Trans-Activators / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics*; D014764:Viral Proteins
C000715470:MAS1 protein, human; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos; D015534:Trans-Activators; D014764:Viral Proteins; C075882:ICP4 protein, Gallid herpesvirus 2; D004815:Epidermal Growth Factor