title: Pathogenesis of Aleutian mink disease parvovirus infection: effects of suppression of antibody response on viral mRNA levels and on development of acute disease.
abstract: We suppressed the B-cell development and antibody response in mink by using treatment with polyclonal anti-immunoglobulin M (anti-IgM) to study the effects of antiviral antibodies on development of Aleutian mink disease parvovirus (ADV)-induced disease in more detail. Newborn mink kits were injected intraperitoneally with 1 mg of either anti-IgM or a control preparation three times a week for 30 to 34 days. At 21 days after birth, groups of mink kits were infected with the highly virulent United isolate of ADV. At selected time points, i.e., postinfection days 9, 13, 29, and 200, randomly chosen mink kits were sacrificed, and blood and tissues were collected for analyses. The efficacy of immunosuppressive treatment was monitored by electrophoretic techniques and flow cytometry. Effects of treatment on viral replication, on viral mRNA levels, and on development of acute or chronic disease were determined by histopathological, immunoelectrophoretic, and molecular hybridization techniques. Several interesting findings emerged from these studies. First, antiviral antibodies decreased ADV mRNA levels more than DNA replication. Second, suppression of B-cell development and antibody response in mink kits infected at 21 days of age resulted in production of viral inclusion bodies in alveolar type II cells. Some of these kits showed mild clinical signs of respiratory disease, and one kit died of respiratory distress; however, clinical signs were seen only after release of immunosuppression, suggesting that the production of antiviral antibodies, in combination with the massive amounts of free viral antigen present, somehow is involved in the induction of respiratory distress. It is suggested that the antiviral antibody response observed in mink older than approximately 14 days primarily, by a yet unknown mechanism, decreases ADV mRNA levels which, if severe enough, results in restricted levels of DNA replication and virion production. Furthermore, such a restricted ADV infection at low levels paves the way for a persistent infection leading to immunologically mediated disease. The potential mechanisms of antibody-mediated restriction of viral mRNA levels and mechanisms of disease induction are discussed.
authors.affiliation: Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University of Copenhagen, Frederiksberg C, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Alexandersen
authors.forename: T
authors.initials: T
authors.lastname: Storgaard
authors.forename: N
authors.initials: N
authors.lastname: Kamstrup
authors.forename: B
authors.initials: B
authors.lastname: Aasted
authors.forename: D D
authors.initials: DD
authors.lastname: Porter
mesh_terms: D000208:Acute Disease; D000453:Aleutian Mink Disease / Q000209:etiology / Q000235:genetics / Q000276:immunology*; D000454:Aleutian Mink Disease Virus / Q000254:growth & development / Q000276:immunology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D000914:Antibodies, Viral / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology; D015152:Blotting, Northern; D015139:Blotting, Southern; D004261:DNA Replication; D005260:Female; D005434:Flow Cytometry; D007075:Immunoglobulin M / Q000276:immunology; D007165:Immunosuppression Therapy; D017403:In Situ Hybridization; D007181:Inclusion Bodies, Viral; D008297:Male; D008907:Mink; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012141:Respiratory Tract Infections / Q000276:immunology / Q000473:pathology; D013997:Time Factors
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007075:Immunoglobulin M; D015347:RNA Probes; D012333:RNA, Messenger; D012367:RNA, Viral; C044203:anti-IgM