Effects of transforming growth factor alpha and beta on rabbit gastric epithelial cell proliferation: a preliminary report.
The effects of epidermal growth factor (EGF), transforming growth factor alpha (TGF alpha), insulin-like growth factor-I (IGF-I), and transforming growth factor beta (TGF beta) on tritiated thymidine incorporation into DNA by rabbit gastric epithelial cells were investigated. EGF, TGF alpha, and IGF-I at concentrations of 10(-10) and 10(-9) M all produced a serum-like stimulatory effect, whereas TGF beta at both concentrations was inhibitory. Coadministration of TGF alpha had no additional effect on EGF-stimulated tritiated thymidine incorporation into DNA. On the other hand, coadministration of TGF beta abolished EGF-stimulated tritiated thymidine incorporation into DNA, suggesting possible interactions between the two growth factors.
Nakajima
N
N
Department of Medicine, Nihon University School of Medicine, Tokyo.
Kuwayama
H
H
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D011817:Rabbits; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D004247:DNA