Presence of HIV-1 in human parenchymal and non-parenchymal liver cells in vivo.
The infection of liver cells by HIV-1 was investigated in vivo. Liver biopsies from 13 anti-HIV-1 antibody-positive patients were studied and HIV-1 DNA was revealed by polymerase chain reaction (PCR) in eight. In situ hybridization demonstrated the presence of HIV-1 RNA in all eight PCR-positive liver specimens. Mononuclear inflammatory cells in the portal tracts and Kupffer cells were labeled by a HIV-1 35S-RNA probe in all cases and by an anti-p24 monoclonal antibody, in seven cases. In addition, hepatocytes also clearly scored positive for HIV-1 RNA in three cases. These results demonstrate the infection of both parenchymal and non-parenchymal liver cells by HIV-1 in vivo and therefore show that HIV-1 can infect an epithelial CD4-negative cell type.
Housset
C
C
INSERM U-370, Paris, France.
Lamas
E
E
Courgnaud
V
V
Boucher
O
O
Girard
P M
PM
Marche
C
C
Brechot
C
C
D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology*; D001483:Base Sequence; D001706:Biopsy; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008099:Liver / Q000737:chemistry / Q000382:microbiology* / Q000473:pathology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
D004279:DNA, Viral; D012367:RNA, Viral