title: Thin-layer chromatographic determination of brain catecholamines and 5-hydroxytryptamine.
abstract: A simple and sensitive (up to nanogram level) method to determine norepinephrine, serotonin and dopamine in rat brain is described. The amines are acetylated and the derivatives are resolved by thin layer chromatography (TLC) on silica high performance thin layer chromatography (HPLC) plates. Quantification is achieved by fluorescence densitometry at 415 nm excitation wavelength.
authors.affiliation: Department de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Alemany
authors.forename: M C
authors.initials: MC
authors.lastname: Nicolau
authors.forename: A
authors.initials: A
authors.lastname: Gamundí
authors.forename: R
authors.initials: R
authors.lastname: Rial
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004298:Dopamine / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D012701:Serotonin / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D002395:Catecholamines; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine