Conservation of the amino acid sequence of SV2, a transmembrane transporter in synaptic vesicles and endocrine cells.
SV2 is a secretory vesicle-specific protein produced by all neurons and by endocrine cells. The deduced amino acid (aa) sequence of this protein indicates that it is a transmembrane transporter [Bajjalieh et al., Science 257 (1992) 1271-1273; Feany et al., Cell 70 (1992) 861-867; Gingrich et al., FEBS Lett. 312 (1992) 115-122]. To determine the regions of the protein that are the most highly conserved throughout evolution, and might therefore be essential for the function of SV2, we isolated a cDNA clone encoding SV2 from the elasmobranch fish, Discopyge ommata, and compared the deduced aa sequence to two isoforms from rat, SV2A and SV2B [Bajjalieh et al., Proc. Natl. Acad. Sci. USA 90 (1993) 2150-2154]. The comparison indicates that although the N-terminal cytoplasmic domain of SV2 is the most divergent region, it contains a highly conserved sequence that is predicted to be the epitope for a monoclonal antibody that crossreacts with all species and two isoforms of SV2 [Buckley and Kelly, J. Cell Biol. 100 (1985) 1284-1294; Bajjalieh et al., Proc. Natl. Acad. Sci. USA 90 (1993) 2150-2154]. The remainder of the protein is highly conserved: 62% of the aa in SV2 from D. ommata are identical to the rat SV2A sequence, and 12% are conservative substitutions. The high degree of conservation of this protein throughout evolution and across species indicates that it mediates a critical function of synaptic vesicles.
Bindra
P S
PS
Department of Neurobiology, Harvard Medical School, Boston, MA 02115.
Knowles
R
R
Buckley
K M
KM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA; D004702:Endocrine Glands / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012860:Skates, Fish; D013572:Synaptic Vesicles / Q000378:metabolism
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C489618:Sv2a protein, rat; D004247:DNA