title: Analysis of the subtelomeric regions of macronuclear gene-sized DNA molecules of the hypotrichous ciliate Stylonychia lemnae: implications for the DNA fragmentation process during macronuclear development?
abstract: The subtelomeric regions of macronuclear gene-sized DNA molecules from Stylonychia lemnae were analyzed. The results obtained indicate that these regions show a highly ordered and common sequence organization: Immediately adjacent to the telomeric sequence a short inverted repeat sequence is found, followed by another 7-9 bp inverted repeat sequence at approximately position 40. A 10 bp consensus sequence found in the subtelomeric regions of all gene-sized DNA molecules is found at approximately position 60 and in addition at about the same position palindromic sequences showing no homology to each other are localized. The biological significance of this sequence organization is discussed.
authors.affiliation: Medizinisch-Naturwissenschaftliches Forschungszentrum, Universität Tübingen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Maercker
authors.forename: H J
authors.initials: HJ
authors.lastname: Lipps
mesh_terms: D000818:Animals; D001483:Base Sequence; D016798:Ciliophora / Q000235:genetics* / Q000254:growth & development; D016054:DNA, Protozoan / Q000378:metabolism*; D017125:Genes, Protozoan; D008957:Models, Genetic; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D016615:Telomere / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D016054:DNA, Protozoan