title: Measurement of acetylcholine receptor function in microcircuit-coupled myoblasts.
abstract: An electrophysiological measurement principle for long-term, noninvasive monitoring of the nicotinic acetylcholine receptor (nAChR) function is described. The measurement is based on the ability to record agonist-induced depolarizations of clonal myoblasts that have formed high impedance seals with extracellular microcircuit electrodes. The technique appears promising for several types of assays and environmental monitoring applications.
authors.affiliation: Geo-Centers, Inc., Ft. Washington, MD.
authors.forename: J N
authors.initials: JN
authors.lastname: Quong
authors.forename: T L
authors.initials: TL
authors.lastname: Fare
authors.forename: H J
authors.initials: HJ
authors.lastname: Bryant
authors.forename: D A
authors.initials: DA
authors.lastname: Stenger
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D017097:Electric Impedance; D004594:Electrophysiology / Q000295:instrumentation*; D008322:Mammals; D008839:Microelectrodes; D008991:Monitoring, Physiologic / Q000295:instrumentation; D009132:Muscles / Q000196:embryology / Q000502:physiology; D011950:Receptors, Cholinergic / Q000502:physiology*
chemical_list: D011950:Receptors, Cholinergic