[The use of Miniphage D3112 deltaHcts as an integrative vectorin Pseudomonas aeruginosa cells].
Catechol 2,3-dioxygenase gene (xylE) was cloned into the Pseudomonas aeruginosa miniphage D3112 delta Hcts. Miniphage provides an effective transduction of gene xylE and its transposition into different replicons. It can be used as an integrative vector in the P. aeruginosa cells. Besides, gene xylE is rather convenient genetic marker for identification of miniphage both in Excherichia coli and Pseudomonas species.
Kirsanov
N B
NB
Mar'ina
O V
OV
Ianenko
A S
AS
D001435:Bacteriophages / Q000235:genetics*; D050557:Catechol 2,3-Dioxygenase; D003001:Cloning, Molecular; D049308:Dioxygenases; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D010105:Oxygenases / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D014161:Transduction, Genetic; D016662:Virus Integration / Q000235:genetics*
D005819:Genetic Markers; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase
Ispol'zovanie minifaga D3112 deltaHcts v kachestve integrativnogo vektora v kletkakh Pseudomonas aeruginosa.