Studies on thermophile products. VI. Activation of mouse peritoneal macrophages by bis(2-hydroxyethyl) trisulfide.
The biological effects of a cytotoxic substance (BS-1), isolated from Bacillus stearothermophilus UK563 and identified as bis(2-hydroxyethyl) trisulfide, on elicited mouse peritoneal macrophages induced by a casein injection, were investigated in vitro. BALB/c mouse macrophages treated or pretreated with BS-1 (1-10 micrograms/ml) showed cytotoxicity against syngeneic DBA/2 mouse P815 mastocytoma. BS-1 also showed weak cytotoxicity directly against P815 in the absence of macrophages. BS-1 significantly increased the glucose consumption of macrophages without producing cytotoxicity. This trisulfide compound increased nitric oxide formation, interleukin-1 production and prostaglandin E2 release in macrophages. It did not, however, increase the production of active oxygen species in macrophages, but it reduced cytochrome c in the presence of phagocytes. These results indicate that BS-1 activates macrophages to the cytolytic stage.
Kohama
Y
Y
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Iida
K
K
Tanaka
K
K
Semba
T
T
Itoh
M
M
Teramoto
T
T
Tsujikawa
K
K
Mimura
T
T
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D015232:Dinoprostone / Q000378:metabolism; D000431:Ethanol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D001411:Geobacillus stearothermophilus / Q000737:chemistry*; D007375:Interleukin-1 / Q000096:biosynthesis; D008262:Macrophage Activation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009569:Nitric Oxide / Q000096:biosynthesis; D013440:Sulfides / Q000302:isolation & purification / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000970:Antineoplastic Agents; D003574:Cytochrome c Group; D007375:Interleukin-1; D013440:Sulfides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000431:Ethanol; C077818:bis(2-hydroxyethyl)trisulfide; D015232:Dinoprostone