Measurement of acetylcholine receptor function in microcircuit-coupled myoblasts.
An electrophysiological measurement principle for long-term, noninvasive monitoring of the nicotinic acetylcholine receptor (nAChR) function is described. The measurement is based on the ability to record agonist-induced depolarizations of clonal myoblasts that have formed high impedance seals with extracellular microcircuit electrodes. The technique appears promising for several types of assays and environmental monitoring applications.
Quong
J N
JN
Geo-Centers, Inc., Ft. Washington, MD.
Fare
T L
TL
Bryant
H J
HJ
Stenger
D A
DA
D000818:Animals; D015374:Biosensing Techniques; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D017097:Electric Impedance; D004594:Electrophysiology / Q000295:instrumentation*; D008322:Mammals; D008839:Microelectrodes; D008991:Monitoring, Physiologic / Q000295:instrumentation; D009132:Muscles / Q000196:embryology / Q000502:physiology; D011950:Receptors, Cholinergic / Q000502:physiology*
D011950:Receptors, Cholinergic