[Determination of beta 2-microglobulin in the serum and cerebrospinal fluid using radial immunodiffusion (comparison with the Elisa Pharmacia method)].
The authors elaborated a method of radial immunodiffusion for assessment of beta 2-microglobulin serum and cerebrospinal fluid levels in patients. The method is based on a modified procedure of staining and decolouration. The antibody produced by USOL Co. which was used for the purpose was not modified. Comparison of 90 sera and 27 cerebrospinal fluid samples where also the reference method ELISA Pharmacia was used revealed almost absolute agreement, the correlation coefficient was 0.98. The method is used for clinical monitoring in patients with myelomas and renal insufficiencies.
Králová
E
E
Oddĕlení klinické biochemie, Fakultní nemocnice, Brno.
Novotná
H
H
Adam
Z
Z
D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*; D008297:Male; D008875:Middle Aged; D001613:beta 2-Microglobulin / Q000032:analysis* / Q000134:cerebrospinal fluid
D001613:beta 2-Microglobulin
Stanovení beta 2-mikroglobulinu v séru a likvoru metodou radiální imunodifuze. (Srovnání s referencní metodou Elisa Pharmacia)