Determination of the histological distribution of insulin like growth factor 1 receptors in the rat gut.
The histological distribution of insulin like growth factor 1 (IGF 1) receptors in the rat gut was studied. Immunostaining of IGF 1 receptors identified localisation on the villus epithelium, in the crypts, and in Brunner's glands of the small intestine. These tissues represent areas of high cell growth/differentiation, division, and macromolecular synthesis respectively, which constitute biological activities long associated with IGF 1. Cellular localisation of IGF 1 receptors was seen in the lamina propria by IGF 1 receptor immunostaining and ligand binding of biotinylated IGF 1. IGF 1 receptor immunostaining in the spleen showed receptor localisation to the splenic pulp thus pointing to macrophages as the possible IGF 1 receptor positive cells in the lamina propria. The results further implicate IGF 1 as an important growth factor in gut maintenance.
Ryan
J
J
Children's Research Centre, Our Lady's Hospital for Sick Children, Dublin, Ireland.
Costigan
D C
DC
D000818:Animals; D002011:Brunner Glands / Q000737:chemistry; D004064:Digestive System / Q000737:chemistry*; D007150:Immunohistochemistry; D008297:Male; D008871:Microvilli / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D017526:Receptor, IGF Type 1 / Q000032:analysis*; D014018:Tissue Distribution
D017526:Receptor, IGF Type 1