Identification of amino acid-base contacts in the Myc-DNA complex by site-specific bromouracil mediated photocrosslinking.
Myc binds to a 6 bp 2-fold symmetric DNA site: 5'-C-3A-2C-1G+1T+2G+3-3'. Using site-specific 5-bromouracil mediated photocrosslinking, we show that His336 of Myc contacts, or is close to, the thymine 5-methyl group at 2-fold symmetry-related positions -2 and +2 of the DNA site in the Myc-DNA complex. Our results strongly suggest that homologous amino acids of Myc and Max make equivalent contacts in the respective protein-DNA complexes.
Dong
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Q
Department of Chemistry, Rutgers University, New Brunswick, NJ 08855.
Blatter
E E
EE
Ebright
Y W
YW
Bister
K
K
Ebright
R H
RH
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001976:Bromouracil / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D000596:Amino Acids; D003432:Cross-Linking Reagents; D009711:Nucleotides; D016271:Proto-Oncogene Proteins c-myc; D001976:Bromouracil; D004247:DNA