Glial conditioned medium alters the expression of amyloid precursor protein in SH-SY5Y neuroblastoma cells.
The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons.
Hirose
Y
Y
Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan.
Imai
Y
Y
Nakajima
K
K
Takemoto
N
N
Toya
S
S
Kohsaka
S
S
D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats
D016564:Amyloid beta-Protein Precursor; D017077:Culture Media, Conditioned; D017931:DNA Primers; D012333:RNA, Messenger