Glutathione conjugation with the pyrrolizidine alkaloid, jacobine.
The reaction of glutathione with the epoxide containing pyrrolizidine alkaloid, jacobine, was catalyzed by guinea pig hepatic glutathione-S-transferase enzymes in in vitro experiments; the rate of the reaction in the presence of rat hepatic glutathione-S-transferases did not differ from the non-enzymatic rate. Using ion-pairing liquid chromatography we were able to isolate the conjugate and obtain a daughter ion spectrum using Fast Atom Bombardment Mass Spectrometry together with Collisionally Activated Dissociation/Mass Analyzed Kinetic Energy experiments.
Dueker
S R
SR
Department of Veterinary Pharmacology and Toxicology, University of California, Davis 95616.
Lamé
M W
MW
Jones
A D
AD
Morin
D
D
Segall
H J
HJ
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D006168:Guinea Pigs; D008658:Inactivation, Metabolic; D008099:Liver / Q000201:enzymology*; D008297:Male; D011763:Pyrrolizidine Alkaloids / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016339:Spectrometry, Mass, Fast Atom Bombardment
D011763:Pyrrolizidine Alkaloids; C085554:glutathione-jacobine conjugate; C013581:jacobine; D005982:Glutathione Transferase; D005978:Glutathione