Induction of oxidative burst response in human neutrophils by immune complexes made in vitro of lipopolysaccharide and hyperimmune serum from chronically infected patients.
Purified lipopolysaccharide (LPS) from Pseudomonas aeruginosa was used as an antigen for immune complex (IC) formation in vitro together with hyperimmune sera from chronically P. aeruginosa-infected patients with cystic fibrosis (CF). P. aeruginosa LPS by itself did not induce an oxidative burst in human neutrophil granulocytes (PMN)s measured by chemiluminescence (CL). This was also the case using hyperimmune CF serum alone. In contrast, P. aeruginosa LPS together with CF serum did induce a CL response. The CL responses varied depending on the sera used for IC formation, and were reduced when protein A preabsorbed sera were used. PEG precipitation of the ICs from the mixture increased the CL response. These findings indicate that the CL responses induced by the mixture of P. aeruginosa LPS and CF serum were due to IC formation and an Fc-mediated stimulation of the PMNs. It is concluded that ICs made from sera of chronically infected CF patients and purified P. aeruginosa LPS are biologically active in terms of activating PMNs, and may contribute to the lung tissue damage seen in this group of patients.
Kronborg
G
G
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Fomsgaard
A
A
Jensen
E T
ET
Kharazmi
A
A
Høiby
N
N
D000907:Antibodies, Bacterial / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology*; D003550:Cystic Fibrosis / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D016897:Respiratory Burst; D013205:Staphylococcal Protein A / Q000378:metabolism
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D008070:Lipopolysaccharides; D013205:Staphylococcal Protein A