Immunogenicity studies of a synthetic antigen of alpha methyl dopa.
Since the idiosyncratic liver toxicity of methyl dopa (L-alpha-methyl-3,4- dihydroxy-phenylalanine) may be due to immune mediated mechanisms, immunologic tools are needed to detect methyl dopa induced antibody and antigen. Hapten (methyl dopa)--carrier (albumin) conjugates were synthesized to generate antibodies reactive with this drug. Studies were also conducted to test the immunogenicity of this hapten-carrier conjugate and its cross reactivity with methyl catechol and levodopa. Methyl dopa (MD), levodopa (LD) or methyl catechol (MC) were conjugated to rabbit serum albumin (RSA) under high pH (base) conditions or by a tyrosinase (tyr) catalyzed reaction. Under the base conjugation conditions, MD-RSA, LD-RSA and MC-RSA conjugates were produced at higher hapten: carrier ratios than conjugates produced by the enzyme catalyzed reaction. Rabbits were subsequently immunized with either MD-RSA(base) or MD-RSA(tyr). Antibodies elicited by MD-RSA(base) had marked reactivity to the carrier protein, albumin, whereas antibodies elicited by MD-RSA(tyr) did not. In addition, reactivity of anti-MD antibody was equal to or greater with MC-RSA than reactivity with either MD-RSA or LD-RSA. This work suggests that the conjugation method using the tyr catalyzed reaction produces the optimal immunogen with minimal modification of the carrier protein. In addition, the catechol moiety of MD, MC and LD appears to be the immunogenic epitope on these haptens.
Hubbard
A K
AK
School of Pharmacy, University of Connecticut, Storrs, CT.
Lohr
C L
CL
Hastings
K
K
Clarke
J B
JB
Gandolfi
A J
AJ
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D001323:Autoantibodies / Q000096:biosynthesis; D015551:Autoimmunity / Q000187:drug effects; D002396:Catechols / Q000276:immunology; D006241:Haptens; D007114:Immunization; D007980:Levodopa / Q000276:immunology; D008099:Liver / Q000187:drug effects / Q000276:immunology; D008297:Male; D008750:Methyldopa / Q000276:immunology* / Q000633:toxicity; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology
D000941:Antigens; D001323:Autoantibodies; D002396:Catechols; D006241:Haptens; D012709:Serum Albumin; C018599:4-methylcatechol; D007980:Levodopa; D008750:Methyldopa