title: A novel stage-specific differentiation antigen is expressed on mouse testicular germ cells during early meiotic prophase.
abstract: A murine cell surface antigen exhibiting stage-specific expression during spermatogenesis was detected with two monoclonal antibodies (mAbs), designated BC7 and CA12. In mouse testis, these mAbs recognized a small population of cells located near the periphery of seminiferous tubules at stages XII and I-VI, and these spermatogenic cells were identified as zygotene and early pachytene spermatocytes. Expression of the antigens was transient and was not detected in germ cells at more advanced stages of spermatogenesis such as late pachytene spermatocytes and round spermatids. Immunoprecipitation and immunoblotting studies showed that both mAbs CA12 and BC7 reacted with the same antigenic molecule, which had an estimated molecular mass of 95 kDa. CA12/BC7 antigen, detected in plasma membrane fraction, was a glycoprotein with sialic acid residues and had affinity with WGA lectin. Furthermore, intraperitoneal injection of mAb BC7 caused an apparent spermatogenic disturbance in prepubertal mice. These results suggested that CA12/BC7 antigen, a novel cell surface glycoprotein, is an essential molecule that plays an important role during early meiotic prophase of spermatogenesis.
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: U
authors.initials: U
authors.lastname: Koshimizu
authors.forename: D
authors.initials: D
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Sawada
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimune
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000943:Antigens, Differentiation / Q000378:metabolism*; D015415:Biomarkers; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008540:Meiosis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011418:Prophase / Q000276:immunology; D051381:Rats; D013091:Spermatogenesis / Q000276:immunology*; D013737:Testis / Q000166:cytology / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D015415:Biomarkers