[Experience with the molecular genetical detection of the chimera gene of the Philadelphia chromosome].
Molecular genetical techniques could be developed for detection of the chimera gene of Philadelphia chromosome or that of its gene product, due to the relatively conserved structure of the chimera gene. The authors successfully analysed 123 blood/bone marrow samples from 106 patients using these molecular techniques adapted from the literature. Patients were classified by the first diagnosis, 65 CML, 7 AML, 13 ALL patients were studied. 12 patients had the diagnosis of myeloproliferative syndrome, and 9 patients were after bone marrow transplantation. 57% of the total, and by diagnosis, 74% of CML, 28% of AML, 54% of ALL, and 33% of post-transplant samples have shown the chimera gene structure characteristic for Philadelphia chromosome. All patients of myeloproliferative syndrome were negative. In some cases the authors had the opportunity to study simultaneously the peripheral blood and the bone marrow sample of the same patient and of the same date. The ratio of the positivity of the two samples varied from one to infinite. The authors could follow the effect of interferon in one case, the change of clonality of the leukemic cell line in an other case. They had the opportunity to detect two different abnormal gene structures in the sample of an AML patient.
Földi
J
J
Országos Haematologiai, Vértranszfúziós és Immunológiai Intézet, Budapest.
Páldi Haris
P
P
Poros
A
A
Fekete
S
S
Pálóczi
K
K
Demeter
J
J
Dénes
R
R
Mód
A
A
Adám
E
E
Tamáska
J
J
D002678:Chimera; D003582:Cytogenetics; D005260:Female; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics*; D008297:Male; D008967:Molecular Biology; D009196:Myeloproliferative Disorders / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics*; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics*
Philadelphia chromosoma chimera génje molekuláris genetikai kimutatásának tapasztalatai.