Effect of 12-O-tetradecanoylphorbol-13-acetate on expression of cell surface antigens in two subclones of human leukemia K562 cells.
Two subclones (K562-L and -H) were previously isolated from K562 human leukemic cells according to hemoglobin production: K562-L was expressed in less than 5% and K562-H in more than 90% of dianisidine positive cells. 12-O-Tetradecanoylphorbol-13- acetate (TPA) suppressed the expression of the erythrocytic (glycophorin A) and myelocytic (CD11b) antigens in K562-L, but increased the expression of these antigens in K562-H. TPA increased the megakaryocytic (CD61) antigens in both cells. These findings suggest that there are distinct TPA responsible factors in K562-L and -H on the expression of the erythrocytic and myelocytic antigens.
Sasaki
D
D
Department of Microbiology, Tohoku College of Pharmacy, Sendai, Japan.
Kosunago
S
S
Mikami
T
T
Matsumoto
T
T
Suzuki
M
M
D000954:Antigens, Surface / Q000032:analysis*; D006021:Glycophorins / Q000032:analysis*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000032:analysis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D006021:Glycophorins; D016177:Macrophage-1 Antigen; D013755:Tetradecanoylphorbol Acetate