Isolation and sequencing of deer and sheep insulin-like growth factors-I and -II.
We have developed a simple method for the isolation of highly purified cervine (c) and ovine (o) insulin-like growth factors-I (IGF-I) and -II. The IGFs were isolated from acidified serum by cation exchange chromatography and then purified by gel filtration, chromatofocusing, and reverse-phase chromatography. The IGF preparations are > 95% pure. The cIGF-I preparation contains < 0.056% cIGF-II and the oIGF-I preparation contains < 0.01% oIGF-II. Both the IGF-II preparations contain < 0.01% IGF-I. The amino acid sequence of cIGF-I has two differences when compared with human (h) IGF-I. The cIGF-II sequence, which is identical to bovine IGF-II, has three differences when compared with hIGF-II.
Moore
L G
LG
Wallaceville Animal Research Centre, AgResearch, Upper Hutt, New Zealand.
Jones
D
D
Lymburn
M A
MA
Hodgkinson
S C
SC
Davis
S R
SR
Suttie
J M
JM
Sadighi
M
M
Carne
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003670:Deer / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011863:Radioimmunoassay; D011869:Radioligand Assay; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000378:metabolism*
D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II