Lipids alter the level and distribution of mouse mammary tumor virus gp52 in vitro.
Linoleic acid, cholesterol, dexamethasone and progesterone were tested by immunocytochemistry and immunoprecipitation for their single and combined effects in vitro on mouse mammary tumor virus (MMTV) gp52 distribution among three compartments: cell-associated antigen, extracellular virus particles and extracellular shed antigen unassociated with virus particles. Results indicated that all additives significantly increased total MMTV gp52 levels and altered the distribution. Linoleic acid and dexamethasone induced the greatest relative proportion of extracellular gp52, whereas cholesterol and progesterone induced the greatest proportion of cell-associated gp52. The implications of these findings for the immune response to mammary tumors is discussed.
Sunzeri
F J
FJ
School of Public Health, University of California, Berkeley 94720.
Buehring
G C
GC
D000704:Analysis of Variance; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000378:metabolism*; D002784:Cholesterol / Q000633:toxicity*; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000633:toxicity*; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000382:microbiology*; D008324:Mammary Tumor Virus, Mouse / Q000187:drug effects*; D051379:Mice; D011233:Precipitin Tests; D011374:Progesterone / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000382:microbiology; D014759:Viral Envelope Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D000957:Antigens, Viral, Tumor; D006023:Glycoproteins; D008041:Linoleic Acids; D014759:Viral Envelope Proteins; C040612:glycoprotein 52 antigen, Mouse mammary tumor virus; D011374:Progesterone; D003907:Dexamethasone; D002784:Cholesterol; D019787:Linoleic Acid