[Synthesis of thioacetamide analogs of blood group specific trisaccharide A and its fragments].
The determinant trisaccharide of the blood group A, GalNAc alpha 1-3(Fuc alpha 1-2)Gal beta, was modified by treatment of the trisaccharide acetate with phosphorus pentasulphide to convert its acetamide fragment determining the serological difference between antigenic determinants A and B into the thioacetamide derivative. Thionated analogues of the A disaccharide and of GalNAc alpha-glycoside were obtained in a similar way. Interaction of three monoclonal anti-A antibodies with modified antigens was studied using ELISA, the O-->S replacement allowing to evaluate the contribution of the acetamido carbonyl group into the antigen-antibody interaction.
Shipova
E V
EV
Korchagina
E Iu
EIu
Zemlianukhina
T V
TV
Galanina
O E
OE
Bovin
N V
NV
D000017:ABO Blood-Group System / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D013853:Thioacetamide / Q000737:chemistry*; D014312:Trisaccharides / Q000737:chemistry* / Q000276:immunology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D014312:Trisaccharides; D013853:Thioacetamide
Sintez tioatsetamidnykh analogov gruppospetsificheskogo trisakharida krovi A i ego fragmentov.