Site-specific labelling of the oligosaccharide chains of antibodies.
This paper presents a new method for site-specific labelling of antibodies employing enzymatic reactions without oxidizing or reducing agents. IgG was first treated with immobilized sialidase from Clostridium perfringens to cleave bound NeuAc. CMP-9-deoxy-9-salizoyl-NeuAc, an activated sialic acid analogue, was labelled with 131I via the iodogen-method in high yields (> 95%). Then the oligosaccharide chains of antibodies were labelled yield with the radioactive NeuAc analogue by transfer using alpha-2,6-sialyltransferase from rat liver in 50%.
Schwarz
U
U
Institut für Analytische Chemie, Technische Universität Dresden, Germany.
Wunderlich
G
G
Brossmer
R
R
D000818:Animals; D000906:Antibodies; D002250:Carbon Radioisotopes; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009439:Neuraminidase / Q000378:metabolism; D009844:Oligosaccharides; D051381:Rats; D012794:Sialic Acids; D012799:Sialyltransferases / Q000378:metabolism; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D000906:Antibodies; D002250:Carbon Radioisotopes; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D009844:Oligosaccharides; D012794:Sialic Acids; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D012799:Sialyltransferases; D009439:Neuraminidase; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase