High-performance liquid chromatographic determination of nalidixic acid in rat serum, brain and cerebrospinal fluid.
A high-performance liquid chromatographic method for the determination of nalidixic acid (NA) in rat serum, brain and cerebrospinal fluid (CSF) was developed. NA in rat serum and brain homogenate was extracted and injected onto a reversed-phase column. CSF was directly analysed without extraction procedure. The limits of detection were 0.05 microgram ml-1 for serum, 0.07 microgram g-1 for brain and 0.02 microgram ml-1 for CSF, respectively. Calibration curves were linear over the concentration ranges 0.1-50 micrograms ml-1 for serum, 0.12-9 micrograms g-1 for brain and 0.05-10 micrograms ml-1 for CSF, respectively. The reproducibility of NA assay in rat biological media ranged from 1 to 4% relative standard deviations (RSD). The recoveries of NA added to serum and brain were higher than 96% with an RSD of less than 4%. The present method was found to be applicable to pharmacokinetic study of NA in rat serum, brain and CSF.
Ichikawa
N
N
Department of Pharmacy, Shimane Medical University Hospital, Japan.
Naora
K
K
Hayashibara
M
M
Iwamoto
K
K
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008297:Male; D009268:Nalidixic Acid / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics*; D015363:Quinolones / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
D015363:Quinolones; D009268:Nalidixic Acid