LlaAI and LlaBI, two type-II restriction endonucleases from Lactococcus lactis subsp. cremoris W9 and W56 recognizing, respectively, 5'-/GATC-3' and 5'-C/TRYAG-3'.
Two type-II restriction endonucleases have been purified from Lactococcus lactis subsp. cremoris W9 and W56, the strains isolated from a mixed Cheddar starter. Their characterization showed that LlaAI was an isoschizomer of MboI from Morexella bovis with the cleaving sequence, 5'/GATC-3', being sensitive to methylation of the adenine residue; LlaBI was an isoschizomer to SfcI from Streptococcus faceium with the cleaving sequence, 5'-C/TRYAG-3'. Both LlaAI and LlaBI restriction-modification (R-M) systems are encoded by the plasmids, respectively, pFW094 and pJW563, protecting the harboring strain against phage attack.
Nyengaard
N
N
Royal Veterinary and Agricultural University, Department of Dairy and Food Science, Frederiksberg, Denmark.
Vogensen
F K
FK
Josephsen
J
J
D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D013294:Lactococcus lactis / Q000201:enzymology*; D013379:Substrate Specificity
C085497:endodeoxyribonuclease SfcI; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases