title: Rapid determination of glutamine in biological samples by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method for the determination of glutamine in biological samples is presented. Glutamine was derivatized with ortho-phthalaldehyde reagent containing 3-mercaptopropionic acid and separated by reversed phase chromatography on a C18 column containing 3-microns particles. No interference from other amino acids was observed. The assay was linear over a range from 1 to 2,000 mumol/l. Analytical recovery of plasma samples spiked with glutamine was 98.6 +/- 3.8%. Within- and between-batch imprecision were 1.5% and 2.2%, respectively. The derivatization step was fully automated. Total analysis time, including derivatization and chromatography, amounted to 6 min. The method can be used for the determination of glutamine in plasma, urine, cerebrospinal fluid and tissue homogenates.
authors.affiliation: Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: T
authors.initials: T
authors.lastname: Teerlink
authors.forename: M W
authors.initials: MW
authors.lastname: Hennekes
authors.forename: P A
authors.initials: PA
authors.lastname: van Leeuwen
authors.forename: A
authors.initials: A
authors.lastname: Houdijk
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D005973:Glutamine / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D006801:Humans; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds; D009764:o-Phthalaldehyde
chemical_list: D013438:Sulfhydryl Compounds; D005973:Glutamine; D009764:o-Phthalaldehyde; C023884:thiolactic acid