Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen.
A cDNA clone, Sta 44-4, corresponding to a mRNA highly expressed in Brassica napus cv. Westar stamens, was isolated by differential screening and characterized. Northern blot and in situ analyses demonstrated that Sta 44-4 is synthesized in pollen beginning at the late uninucleate stage and reaches a maximum in trinucleate microspores. Sta 44-4 displayed significant sequence similarity to known pollen polygalacturonase genes. The B. napus pollen polygalacturonase gene was shown to be part of a small gene family and to display some polymorphism among different cultivars.
Robert
L S
LS
Plant Research Centre, Centre Experimental Farm, Ottawa, Ontario, Canada.
Allard
S
S
Gerster
J L
JL
Cass
L
L
Simmonds
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011058:Pollen; D011096:Polygalacturonase / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger; D011096:Polygalacturonase