Increased alpha 1B-adrenoreceptor mRNA levels in the rat kidney after thyroidectomy.
Oligonucleotide probes were designed to sequences of the rat alpha 1B- and alpha 2B-adrenergic receptor mRNA and used for in situ hybridization histochemistry on tissue sections of kidneys from control and thyroidectomized rats. Both alpha 1B- and alpha 2B-receptor mRNA labelling was demonstrated in proximal tubule cells in the outer stripe of the outer medulla, with tubular rays radiating into the cortex. Thyroidectomy induced a more than 4-fold increase in mRNA for the alpha 1B-receptor in the kidney, whereas no change in alpha 2B-receptor mRNA levels could be demonstrated in thyroidectomized rats as compared to control animals. The results suggest that thyroid hormone plays an important role in regulating expression of alpha 1B-receptors in renal tubule cells.
Meister
B
B
Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
Dagerlind
A
A
Nicholas
A P
AP
Hökfelt
T
T
D000818:Animals; D001345:Autoradiography; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000033:anatomy & histology / Q000378:metabolism; D008297:Male; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D013965:Thyroidectomy
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1