title: Biochemical characterisation of Trichinella spiralis and T. pseudospiralis stichocyte antigens.
abstract: One and two dimensional Western blots of Trichinella spiralis and Trichinella pseudospiralis extracts were probed with a monoclonal antibody (7C2C5) which recognizes molecules found in the stichocytes. Three major antigens were revealed in excreted:secreted (E/S) preparations at approx Mr 45, 53 & 60 X 10(3). The Mr 45 X 10(3) molecule was the most abundant secreted antigen, on two dimensional analysis it resolved into many isoforms with a pI range 4.5-6.4 in T. spiralis and into four isoforms pI range 5.1-5.7 in T. pseudospiralis.
authors.affiliation: Department of Pure and Applied Biology, University of Leeds, United Kingdom.
authors.forename: Y W
authors.initials: YW
authors.lastname: Zhang
authors.forename: D L
authors.initials: DL
authors.lastname: Lee
authors.forename: J E
authors.initials: JE
authors.lastname: Smith
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D007519:Isoantigens / Q000032:analysis; D007814:Larva / Q000276:immunology; D051379:Mice; D014234:Trichinella / Q000276:immunology*; D017160:Trichinella spiralis / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D007519:Isoantigens