title: The immunodominant 38-kDa lipoprotein antigen of Mycobacterium tuberculosis is a phosphate-binding protein.
abstract: Several antigens of Mycobacterium tuberculosis have been identified by monoclonal antibodies and are being exploited in the development of improved vaccines and diagnostic reagents, but none has been linked to a specific function. Herein we report that the 38-kDa extracellular lipoprotein antigen, the most potent immunogen of the mycobacteria, is a phosphate-binding protein with features very similar to those of the well characterized periplasmic phosphate-binding protein of Escherichia coli which serves as an initial receptor for active transport. This is also the first report definitively linking a function of a binding protein anchored to a membrane and found in other than Gram-negative bacteria.
authors.affiliation: Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: Z
authors.initials: Z
authors.lastname: Chang
authors.forename: A
authors.initials: A
authors.lastname: Choudhary
authors.forename: R
authors.initials: R
authors.lastname: Lathigra
authors.forename: F A
authors.initials: FA
authors.lastname: Quiocho
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005798:Genes, Bacterial; D006860:Hydrogen Bonding; D008074:Lipoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D028044:Phosphate-Binding Proteins; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: C075966:Ag38 antigen, Mycobacterium tuberculosis; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D002352:Carrier Proteins; D017931:DNA Primers; D008074:Lipoproteins; D008565:Membrane Proteins; D028044:Phosphate-Binding Proteins; D011994:Recombinant Proteins