A general extracorporeal immunoadsorption method to increase tumor-to-tissue ratio.
The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios were increased in various tissues (i.e., radiosensitive and blood rich organs) by a factor of four to five.
Strand
S E
SE
Department of Radiation Physics, Lund University, Sweden.
Norrgren
K
K
Garkavij
M
M
Lindgren
L
L
Nilsson
R
R
Sjogren
H O
HO
Tennvall
J
J
D000818:Animals; D001360:Avidin; D001710:Biotin; D005112:Extracorporeal Circulation; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D007457:Iodine Radioisotopes / Q000627:therapeutic use*; D008175:Lung Neoplasms / Q000532:radiotherapy*; D008297:Male; D008545:Melanoma / Q000532:radiotherapy*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016499:Radioimmunotherapy / Q000379:methods*; D014183:Transplantation, Heterologous
D007457:Iodine Radioisotopes; D001360:Avidin; D001710:Biotin