[The hierarchy of complexes and compact structures of trivaline with nucleic acids. III. Complexes of trivaline with trinucleotides forms a rod-like structure with length of about 1000 A in solution].
We demonstrated the ability of trivaline in the course of interaction with certain trinucleotides in solution to form extended fibre-like structures with lengths of up to several thousand angstroms. Such structures were observed for complexes of trivaline with both deoxyribo- and ribonucleotides with homopurine, homopyrimidine, or random sequences, with or without terminal 5'-phosphate. A model of organization of such structures is proposed. It is based on tetramer complex of trivaline with short nucleotides, two structural units of which, consisting of trivaline tetramer and two trinucleotides, form the octamer complex. It has three perpendicular axes of symmetry of the second order. The spatial location of bases in this structure is additionally fixed by nucleopeptide interactions. The latter create favourable conditions for arranging hydrogen bonds between trinucleotides belonging to different tetramer complexes and stacking interactions between the bases of each nucleotide. Octamer complexes are able to form regular aggregates in the form of a "stack", consisting of dozens of elementary units. These aggregates can be electron microscopically visualized as extended fibre-like structures.
Strel'tsov
S A
SA
Martynkina
L P
LP
Florent'ev
V L
VL
Vengerov
Iu Iu
IuIu
Zhenodarova
S M
SM
Sedel'nikova
E A
EA
Smolianinova
O A
OA
D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D011487:Protein Conformation; D014633:Valine / Q000737:chemistry*
D009841:Oligonucleotides; D014633:Valine
Ob ierarkhii kompleksov i kompaktnykh struktur trivalina s nukleinovymi kislotami. III. Kompleksy trivalina s trinukleotidami obrazuiut v rastvore palochkoobraznye struktury dlinoĭ 1000 A]