title: Characterization of insoluble macromolecular Sn(II) complex and its application to the 99mTc labeling of human serum albumin-bearing mercapto groups.
abstract: A chelating ion exchange resin containing aminophosphonic acid groups was used as a polymer matrix for the preparation of an insoluble macromolecular Sn(II)(R-Sn) complex. Sn(II), which strongly bound to the surface of the polymer matrix by chelation, retained the ability to reduce 99mTc in the R-Sn complex. Human serum albumin-bearing-mercapto groups (HMA) was labeled with 99mTc at pH 2-3 using the R-Sn complex or SnCl2 as reducing agent. The 99mTc-HMA labeled with the R-Sn complex resulted in a higher sustained level of radioactivity in the blood of mice than the 99mTc-HMA labeled with SnCl2. These results suggest that the use of the R-Sn complex minimized Sn(II) contamination of the 99mTc labeling solution and can be used effectively as a reducing agent for 99mTc labeling of proteins containing sulfhydryl groups.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Terahara
authors.forename: R
authors.initials: R
authors.lastname: Ikeda
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: T
authors.initials: T
authors.lastname: Koiso
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Harada
authors.forename: A
authors.initials: A
authors.lastname: Sugii
authors.forename: S
authors.initials: S
authors.lastname: Tomiguchi
authors.forename: A
authors.initials: A
authors.lastname: Kojima
mesh_terms: D000818:Animals; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D013438:Sulfhydryl Compounds; D013668:Technetium Tc 99m Aggregated Albumin; D014001:Tin
chemical_list: D046911:Macromolecular Substances; D013438:Sulfhydryl Compounds; D013668:Technetium Tc 99m Aggregated Albumin; D014001:Tin