title: DNA probes for identification of the ectomycorrhizal fungus Tuber magnatum Pico.
abstract: The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), consistently appearing when genomic DNA was amplified with an aspecific primer (OPA-18), was found to be a good marker for Tuber magnatum, and was used as a probe in Southern hybridization experiments. The specificity of the results suggests that this probe may be useful in developing specific primers for PCR amplifications.
authors.affiliation: Centro di Studio sulla Micologia del Terreno del CNR, Torino, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Lanfranco
authors.forename: P
authors.initials: P
authors.lastname: Wyss
authors.forename: C
authors.initials: C
authors.lastname: Marzachí
authors.forename: P
authors.initials: P
authors.lastname: Bonfante
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis*; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D004271:DNA, Fungal