Development of a spectrofluorimetric method for determining the pKa of pterin-analogue ligands bound to DHFR.
Jeong
S S
SS
Department of Biochemistry, University of Sydney, NSW, Australia.
Gready
J E
JE
D002364:Caseins / Q000494:pharmacology; D005493:Folic Acid Antagonists / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009249:NADP / Q000378:metabolism; D011622:Pterins / Q000378:metabolism* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*
C085591:6,8-dimethyl-N(5)-deazapterin; D002364:Caseins; D005493:Folic Acid Antagonists; D011622:Pterins; D011994:Recombinant Proteins; D009249:NADP; D013762:Tetrahydrofolate Dehydrogenase