Comparison of the binding sites of plant ferredoxin for two ferredoxin-dependent enzymes.
Differential chemical modification of acidic residues was used to map the binding site of plant ferredoxin (Fd) for the chloroplast enzyme ferredoxin:thioredoxin reductase (FTR). Binding of FTR to Fd inhibits chemical modification of Fd residues D34, D65, E92, E93, E94 and C-terminal A97. The binding site demarcated by these residues differs from that for ferredoxin:NADP+ reductase (FNR). The FTR site includes C-terminal residues but not helix 24-31, which is part of the FNR site. Both sites enclose the [2Fe-2S] cluster.
De Pascalis
A R
AR
Biochemisches Institut, Universität Zürich, Switzerland.
Schürmann
P
P
Bosshard
H R
HR
D001665:Binding Sites; D002736:Chloroplasts / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry* / Q000378:metabolism; D005288:Ferredoxins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D007506:Iron-Sulfur Proteins / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D010944:Plants / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013654:Taurine; D014316:Tritium
D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D014316:Tritium; D013654:Taurine; D010088:Oxidoreductases; C021588:ferredoxin-thioredoxin reductase; D005287:Ferredoxin-NADP Reductase