Phosphorus-31 nuclear magnetic resonance in vivo spectroscopy of human liver during hepatitis A virus infection.
To evaluate the changes in hepatocellular phospholipid metabolism during hepatitis virus infection, 26 patients with acute viral hepatitis A were studied by means of phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy. The spectroscopy of liver showed six signal components in all patients as well as in the normal volunteers. During the early phase of illness, the phosphomonoester (PME)-phosphodiester (PDE) ratios in the patients became markedly greater than the ratios in the controls (P < 0.001). Within six weeks after the onset, the PME/PDE ratios returned to the level of controls. The time course analysis indicated an inverse correlation between the PME/PDE ratio and the period of time after onset (r = 0.738, P < 0.001). The spectral changes of human liver observed in acute viral hepatitis A are similar to those in the regenerating rat liver, indicating that 31P-NMR spectroscopy allows a noninvasive study of cell turnover in human liver disease associated with acute virus infection.
Yamane
Y
Y
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Umeda
M
M
O'uchi
T
T
Mitsushima
T
T
Nakata
K
K
Nagataki
S
S
D000208:Acute Disease; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D005260:Female; D006506:Hepatitis A / Q000175:diagnosis / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
D010710:Phosphates; D010743:Phospholipids; D000255:Adenosine Triphosphate; D000410:Alanine Transaminase; D001663:Bilirubin