Novel Pseudomonas product stimulates interleukin-8 production in airway epithelial cells in vitro.
Because high concentrations of IL-8 are found in the sputum of cystic fibrosis patients, we hypothesized that Pseudomonas aeruginosa (PA) induces the production of IL-8 in airway epithelial cells and in monocytes. Therefore, we incubated the supernatant from PA culture with human transformed bronchial epithelial cells (16-HBE) or with monocytes. The culture medium of 16-HBE cells that had been incubated with PA supernatant for 6 h had chemotactic activity that was inhibited by an antibody to human IL-8. The PA supernatant induced IL-8 production by primary bronchial epithelial cells, by 16-HBE cells, and by monocytes. After incubation with PA supernatant, 16-HBE cells showed a marked increase in the levels of IL-8 gene expression. The PA product responsible for IL-8 production resisted freezing, boiling, and proteolysis. This product was not lipid extractable and was present in a 1-kD filtrate. We conclude that a small molecular mass product of PA stimulates IL-8 production by 16-HBE cells and by monocytes, and that the chemotactic activity produced by 16-HBE cells after exposure to PA is due principally to IL-8.
Massion
P P
PP
Cardiovascular Research Institute, University of California, San Francisco 94143-0130.
Inoue
H
H
Richman-Eisenstat
J
J
Grunberger
D
D
Jorens
P G
PG
Housset
B
B
Pittet
J F
JF
Wiener-Kronish
J P
JP
Nadel
J A
JA
D015152:Blotting, Northern; D001980:Bronchi / Q000378:metabolism*; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D003470:Culture Media; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D009000:Monocytes / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013045:Species Specificity
D003470:Culture Media; D016209:Interleukin-8; D012333:RNA, Messenger