[Recognition of T-T cyclobutane pyrimidine dimers in the form of primers by DNA polymerases].
A study was made of the efficiency of primer conversion catalyzed by human placenta DNA polymerase on the length of primer. The dependence of -log Kd and Vmax on the number of mononucleotide units (n) of primer were shown to be linear up to n <--> 10. Each mononucleotide unit of the primer enhanced its affinity by a factor of 2.3 and the maximal polymerization rate by a factor of 1.3. For the first time estimation was made for the contribution of replication enzymes: human placenta DNA polymerase and E. coli DNA polymerase I (as well as AMV reverse transcriptase) to a decrease in the amount of T-T dimers in DNA emerging after UV- or gamma-irradiation. The efficiency of elongation of the d(pT)10 primer was shown to decrease with an increase in T-T dimers in the primer. When the d(pT)10 primer contains about 2.6 T-T dimers per molecule, the efficiency of its elongation decreases by a factor of 8-18.
Kolocheva
T I
TI
Lokhova
I A
IA
Tansykbaev
T A
TA
Gorn
V V
VV
Nevinskiĭ
G A
GA
D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D010920:Placenta / Q000201:enzymology; D011740:Pyrimidine Dimers / Q000378:metabolism*; D013379:Substrate Specificity
D017931:DNA Primers; D011740:Pyrimidine Dimers; D004259:DNA-Directed DNA Polymerase
Uznavanie DNK-polimerazami T-T tsiklobutanovykh pirimidinovykh dimerov v sostave praĭmerov.