Solid phase radioimmunoassay for the determination of aldosterone excretion.
A solid phase radioimmunoassay (SP-RIA) was developed for the determination of urinary conjugated aldosterone. Acid-hydrolyzed urine was extracted with dichloromethane and the extract was analyzed by SP-RIA. Microtiter plates were coated with two different anti-aldosterone antisera (S-3: against aldosterone-21-hemisuccinate-BSA and 58: aldosterone-3-(O-carboxymethyl)-oxime-BSA separately and preincubated with 3H-aldosterone. Reference values were obtained using a liquid radioimmunoassay method including paper chromatography. Regression analysis revealed for S-3: Y = 0.8 X + 3.2, r = 0.84; p < 0.0001; n = 36 and for 58: Y = 1.16 X + 1.2, r = 0.89; p < 0.0001; n = 36. The SP-RIA assay is useful for routine measurement of conjugated aldosterone excretion. The method is time-saving and does not require paper chromatography, and is adequate for many clinical purposes.
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Department of Medical Chemistry, Albert Szent-Györgyi Medical University, Szeged, Hungary.
D000450:Aldosterone / Q000276:immunology / Q000302:isolation & purification / Q000652:urine*; D000818:Animals; D000918:Antibody Specificity; D002854:Chromatography, Paper; D005965:Glucuronates / Q000652:urine; D006801:Humans; D007106:Immune Sera; D011863:Radioimmunoassay; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep
D005965:Glucuronates; D007106:Immune Sera; D000450:Aldosterone