title: Isolation and sequence of a rat glucose-6-phosphate dehydrogenase promoter.
abstract: A 935 bp fragment of the rat glucose-6-phosphate dehydrogenase (G6PDH) gene containing promoter activity was isolated using the polymerase chain reaction (PCR). This fragment was sequenced and primer extension analysis showed a transcription initiation site in agreement with the human and mouse genes. Computer analysis of the sequence showed a 60% and 78% similarity to the human and mouse G6PDH sequences, respectively. A TATA box element, TTAAAT, was found and shown to be 100% similar to the human and mouse TATA box elements. Based on sequence comparison, some putative transcriptional regulatory elements were also found.
authors.affiliation: Department of Biological Sciences, Western Michigan University, Kalamazoo.
authors.forename: K B
authors.initials: KB
authors.lastname: Rank
authors.forename: P K
authors.initials: PK
authors.lastname: Harris
authors.forename: L C
authors.initials: LC
authors.lastname: Ginsberg
authors.forename: S R
authors.initials: SR
authors.lastname: Stapleton
mesh_terms: D000818:Animals; D001483:Base Sequence; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats
chemical_list: D005954:Glucosephosphate Dehydrogenase