Antisense oligonucleotide to c-fos induces ipsilateral rotational behaviour to d-amphetamine.
The immediate-early genes, especially c-fos, have been suggested to have an important role in neuronal plasticity and memory. However, it has been difficult to determine if in fact c-fos can participate in control of behaviour. Here we can report that direct unilateral infusion into the rat neostriatum of an antisense phosphothioate oligodeoxynucleotide to c-fos mRNA leads to the rapid induction of ipsilateral rotational behaviour after d-amphetamine administration. This is associated with an elimination of the in vivo expression of Fos-like immunoreactivity within the infused region of neostriatum. Following infusion of these oligodeoxynucleotides labelled with fluorescein isothiocyanate they appear with a nuclear and cytoplasmic location in a large number of nerve cells, many of which are immunoreactive for dopamine and cyclic AMP regulated phosphoprotein. The present findings suggest that the immediate-early gene c-fos has a role also in control of behavioural activity, as illustrated in D1 receptor containing striatal neurones.
Sommer
W
W
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Bjelke
B
B
Ganten
D
D
Fuxe
K
K
D000818:Animals; D001483:Base Sequence; D003913:Dextroamphetamine / Q000494:pharmacology*; D016650:Fluorescein-5-isothiocyanate; D016762:Genes, fos; D007150:Immunohistochemistry; D007267:Injections; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D017072:Neostriatum / Q000033:anatomy & histology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013239:Stereotyped Behavior / Q000187:drug effects*
D016376:Oligonucleotides, Antisense; D016650:Fluorescein-5-isothiocyanate; D003913:Dextroamphetamine