Prostaglandin E2 antagonizes gingival fibroblast proliferation stimulated by interleukin-1 beta.
Treatment of human gingival fibroblasts with prostaglandin E2 (PGE2) resulted in significant concentration-dependent inhibition in deoxyribonucleic acid (DNA) synthesis (8.40-37.89%), while indomethacin (INDO) (PG inhibitor), interleukin-1 beta (IL-1 beta) or IL-1 beta+INDO caused a significant and dose-dependent increase in DNA synthesis. Addition of PGE2 to culture media containing IL-1 beta and INDO caused a significant concentration-dependent reduction in IL-1 beta- and INDO-induced stimulation of DNA synthesis. The findings suggest that IL-1 beta and PGE2, which are also produced by fibroblasts, could play an important role in regulation of gingival tissue development and wound healing, and their modulation may have therapeutic potential.
ElAttar
T M
TM
Laboratory of Hormone Research, University of Missouri-Kansas City Schools of Dentistry and Medicine, Kansas City 64108.
Lin
H S
HS
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000187:drug effects*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000494:pharmacology
D007375:Interleukin-1; D011994:Recombinant Proteins; D015232:Dinoprostone; D007213:Indomethacin