Non radioactive single-strand conformation polymorphism (SSCP) analysis of exon 11 of the CFTR gene using the Pharmacia PhastSystem.
The authors report here the use of the PhastSystem (Pharmacia) to perform the single strand conformation polymorphism analysis of polymerase chain reaction products of the exon 11 of the CFTR gene. It provides a rapid (2 hours) safe and reliable technique for the development of carrier testing for individuals or couples with a family history of cystic fibrosis.
Cormier-Daire
V
V
Laboratoire Pol Bouin, INSERM U.314, Hôpital Maison Blanche, CHU, Reims, France.
Clavel
C
C
Polette
M
M
Doco
M
M
Boutterin
M C
MC
Binninger
I
I
Birembaut
P
P
D003550:Cystic Fibrosis / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D006801:Humans; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods
D004277:DNA, Single-Stranded