Detection of established and novel alleles of the HLA-DPB1 locus by PCR-SSO.
The polymorphism at the HLA-DPB1 locus was investigated in 490 Austrian individuals. Exon 2 sequences of the HLA-DPB1 genes were amplified by polymerase chain reaction and probed with a panel of sequence-specific oligonucleotide probes. HLA-DPB1 genotypes were deduced from the hybridization pattern of the probes. HLA-DPB1 allele frequencies and Hardy-Weinberg equilibrium were calculated. Linkage disequilibria between HLA-DPB1 and HLA-DQA1 alleles were determined. Four novel hybridization patterns indicating new alleles were found.
Pickl
W F
WF
Clinical Department for Blood Group Serology, University of Vienna, Austria.
Faé
I
I
Fischer
G F
GF
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping; D005787:Gene Frequency; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D006801:Humans; D015810:Linkage Disequilibrium; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D009841:Oligonucleotides