Lipid peroxidation in the adrenal glands of male rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
This study was performed to determine whether TCDD (50 micrograms/kg; single oral dose) could induce adrenal microsomal lipid peroxidation, which might be correlated to decreased levels of cytochrome P-450 and 21-hydroxylase activity. The amount of malondialdehyde (MDA) formed was significantly higher than controls at days 1 through 5 following TCDD treatment. Microsomal cytochrome P-450 levels were depressed after lipid peroxidation at days 1, 3, and 5, and 21-hydroxylase activity decreased at day 5 after TCDD treatment. This study shows that TCDD stimulates adrenal microsomal lipid peroxidation which is associated with decreased cytochrome P-450 levels and 21-hydroxylase activity.
Bestervelt
L L
LL
School of Public Health, Department of Pharmacology, University of Michigan, Ann Arbor 48109-2029.
Piper
D W
DW
Pitt
J A
JA
Piper
W N
WN
D000311:Adrenal Glands / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013255:Steroid 21-Hydroxylase / Q000378:metabolism
D000072317:Polychlorinated Dibenzodioxins; D008315:Malondialdehyde; D003577:Cytochrome P-450 Enzyme System; D013255:Steroid 21-Hydroxylase