The effect of levamisole on acetylcholinesterase secretion by male and female Heligmosomoides polygyrus during ageing.
The inhibition of acetylcholinesterase secretion by male and female Heligmosomoides polygyrus was tested on worms taken from experimentally infected mice and maintained for 3 days in vitro in levamisole. The dose inhibiting 50% of enzyme secretion (ID 50) of male worms was twice the ID 50 for female worms. A similar difference was observed in vivo between the dose of levamisole removing 50% (LD50) of male and female worms from the mouse. Acetylcholinesterase secretion by worms and ID 50 were tested in vitro at 3-weekly intervals from 3 to 21 weeks post infection (WPI). Acetylcholinesterase secretion was always significantly higher for male than for female worms. A decrease of ID 50, correlated with the age of the worms was observed: from 1.5 to 0.5 micrograms/ml for males and from 0.7 to 0.1 micrograms/ml for females. These results were confirmed in vivo by a higher efficacy of the anthelminthic at 21 than at 4 WPI.
Mallet
S
S
I.N.R.A. Centre de Recherches de Tours, Station de Pathologie Aviaire et de Parasitologie, Nouzilly, France.
Kerboeuf
D
D
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D005260:Female; D007928:Lethal Dose 50; D007978:Levamisole / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D009351:Nematospiroides dubius / Q000187:drug effects* / Q000201:enzymology; D012737:Sex Factors; D017206:Strongylida Infections / Q000188:drug therapy*; D013997:Time Factors
D007978:Levamisole; D000110:Acetylcholinesterase