The atomic structure of Carnation Mottle Virus capsid protein.
The structure of the Carnation Mottle Virus (CMtV) capsid protein has been determined at 3.2 A resolution by the method of molecular replacement. Three-dimensional data were collected from a small number of crystals (sp.g. I23, a = 382.6 A) using the synchrotron radiation with an image plate as detector. The coordinates of Tomato Bushy Stunt Virus (TBSV) were used as a searching model. Refinement of the coordinates of 7,479 non-hydrogen atoms performed by the program XPLOR, has led to an R-factor of 18.3%. It was found that the amino acid chain fold of capsid protein is very similar to that in other icosahedral viruses. However, there are some differences in the contact regions between protein subunits and also the lack of the beta-annulus around the 3-fold icosahedral axes. The structural and biochemical results lead us to consider an alternative assembly pathway.
Morgunova EYu
Institute of Crystallography, Russian Academy of Sciences, Moscow.
Dauter
Z
Z
Fry
E
E
Stuart
D I
DI
Stel'mashchuk VYa
Mikhailov
A M
AM
Wilson
K S
KS
Vainshtein
B K
BK
D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000737:chemistry* / Q000648:ultrastructure; D011487:Protein Conformation; D012328:RNA Viruses / Q000737:chemistry* / Q000648:ultrastructure
D036022:Capsid Proteins; C085702:capsid protein, Carnation Mottle virus