title: Histocompatibility matching for bone marrow transplantation. Donor-recipient pairs in the 4AOHW cell panel.
abstract: While the results of unrelated bone marrow transplantation are continually improving, a number of important issues remain: what are the histocompatibility requirements, what genes are involved, what mismatches are acceptable, and what are the best methods for determining donor-recipient match? In this study of material provided through the 4AOHW and the US NMDP, the match between 53 donor-recipient pairs was determined using several different markers within the MHC. The data showed that many apparently well-matched pairs have many mismatches, including mismatches for non-HLA genes (i.e., non-class-I or non-class-II) within the MHC. New methods matching for blocks of DNA around HLA-B and around HLA-DR/DQ are available that are sensitive and identify additional mismatches that are not apparent using conventional typing methods. The 4AOHW cells provide a valuable resource for the comparison and assessment of new matching techniques.
authors.affiliation: Department of Clinical Immunology, Royal Perth Hospital, Western Australia.
authors.forename: F T
authors.initials: FT
authors.lastname: Christiansen
authors.forename: G
authors.initials: G
authors.lastname: Tay
authors.forename: L K
authors.initials: LK
authors.lastname: Smith
authors.forename: C S
authors.initials: CS
authors.lastname: Witt
authors.forename: E W
authors.initials: EW
authors.lastname: Petersdorf
authors.forename: B
authors.initials: B
authors.lastname: Bradley
authors.forename: R L
authors.initials: RL
authors.lastname: Dawkins
mesh_terms: D001315:Australia; D016026:Bone Marrow Transplantation / Q000276:immunology*; D016172:DNA Fingerprinting; D006086:Graft vs Host Disease / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology; D015235:HLA-B Antigens / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009693:Nucleic Acid Hybridization; D014019:Tissue Donors
chemical_list: D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens