Processing and presentation of tetanus toxin by antigen-presenting cells from patients with chronic granulomatous disease (CGD) to human specific T cell clones are not impaired.
The capacity of peripheral blood lymphocytes (PBL) or Epstein-Barr virus (EBV)-transformed B cell lines from CGD patients to process and present tetanus toxin (tt)-specific epitopes was assessed using various tt preparations and human tt-specific T cell clones. PBL from all of the donors were able to process and present either native tt and/or denatured tt to human T cell clones specific for various tt epitopes. Furthermore, no difference was found in the antigen requirement when normal or CGD EBV-B cell lines were used as antigen-presenting cells (APC). These results suggest that the deficiency in oxygen metabolism in CGD cells does not affect tt processing and presentation.
Barbey
C
C
Institut de Biochimie, Université de Lausanne, Epalinges, Switzerland.
Tiercy
J M
JM
Fairweather
N
N
Niemann
H
H
Seger
R
R
Corradin
G
G
D000595:Amino Acid Sequence; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology; D002461:Cell Line, Transformed; D002999:Clone Cells; D006105:Granulomatous Disease, Chronic / Q000276:immunology*; D004854:Herpesvirus 4, Human; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000276:immunology*; D013744:Tetanus Toxin / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D013744:Tetanus Toxin