title: Evidence for a phosphoenolpyruvate dependent sugar-phosphotransferase system in the mollicute Acholeplasma florum.
abstract: In order to confirm the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose in the presence of PEP. We also cloned and sequenced a DNA fragment coding for a putative polypeptide showing significant similarity with the enzyme II of the beta-glucoside PTS of Escherichia coli. Taken together, these results show that A florum possesses a fructose-PTS, but not a glucose-PTS, and that the amino acid sequence deduced from the DNA fragment is related to beta-glucoside and sucrose enzymes II of PTS from various bacteria.
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique et Université de Bordeaux II, Villenave d'Ornon, France.
authors.forename: J
authors.initials: J
authors.lastname: Navas-Castillo
authors.forename: F
authors.initials: F
authors.lastname: Laigret
authors.forename: A
authors.initials: A
authors.lastname: Hocquellet
authors.forename: C J
authors.initials: CJ
authors.lastname: Chang
authors.forename: J M
authors.initials: JM
authors.lastname: Bove
mesh_terms: D000127:Acholeplasma / Q000201:enzymology*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022360:phosphoenolpyruvate-protein phosphotransferase; D005947:Glucose