Localization of heat shock protein in orbital tissue from patients with Graves' ophthalmopathy using in situ hybridization.
We studied the cellular localization of heat shock protein 70 (HSP70) synthesis in human orbital tissue from patients with Graves' ophthalmopathy(GO) before and after immunosuppressive therapy. Synthesis was detected using in situ hybridization techniques. In a specimen from a GO patient in active phase, strong expression of HSP70 mRNA was detected in infiltrating lymphocytes as well as in fibroblasts. HSP70 mRNA was also detected in orbital tissue fibroblasts from GO patients following treatment, but not in control specimens. HSP70 expression in the orbital tissue of GO patients may be important in mediation of the immune process.
Tanaka
K
K
Department of Medicine, Kurume University School of Medicine, Fukuoka, Japan.
Hiromatsu
Y
Y
Sato
M
M
Inoue
Y
Y
Nonaka
K
K
D000328:Adult; D000368:Aged; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006111:Graves Disease / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009915:Orbit / Q000737:chemistry / Q000503:physiopathology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
D006360:Heat-Shock Proteins; D012333:RNA, Messenger