title: Identification of a cryptic gene associated with an insertion sequence not previously identified in Bacillus thuringiensis.
abstract: After screening several Bt strains with a cryII toxin probe, clones from two strains were found to contain a cryptic cryIIB gene associated with an insertion sequence element belonging to the IS2/IS3 family. The lack of expression of this gene appears to result from mutation of the upstream orf2 gene which has been shown to be necessary for cryII expression.
authors.affiliation: Department of Biochemistry, University of Cambridge, UK.
authors.forename: T C
authors.initials: TC
authors.lastname: Hodgman
authors.forename: Y
authors.initials: Y
authors.lastname: Ziniu
authors.forename: J
authors.initials: J
authors.lastname: Shen
authors.forename: D J
authors.initials: DJ
authors.lastname: Ellar
mesh_terms: D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis