title: Studies on binding of glycosaminoglycans to Streptococcus pyogenes by using 125I-heparan sulphate as a probe.
abstract: Binding of 125I-heparan sulphate to the cell surface of Streptococcus pyogenes is mediated by proteins, that could be released from the streptococcal cell wall by using alkaline buffer. SDS-electrophoresis revealed two bands with molecular weights of 63 and 58 kDa. Binding of the 125I-labelled heparan sulphate probe to streptococci seems to be due to charge interactions, as the same probe was displaced by unlabelled heparan sulphate, other negatively charged molecules such as heparin, dextran sulphate, dermatan sulphate or by high ionic strength. The interaction was also strongly influenced by pH. The binding constant at pH 7.2 was estimated to be 9.8 x 10(6) mol/l, suggesting a moderate affinity. The presence of collagen of different types enhanced binding of 125I-labelled heparan sulphate to streptococci, whereas fibronectin and vitronectin had an inhibitory effect. The cooperation between heparan sulphate and collagen could be important for the adhesion of streptococci to connective tissue.
authors.affiliation: Friedrich Schiller University Jena, Institute of Experimental Microbiology, Germany.
authors.forename: K H
authors.initials: KH
authors.lastname: Schmidt
authors.forename: F
authors.initials: F
authors.lastname: Ascencio
authors.forename: L A
authors.initials: LA
authors.lastname: Fransson
authors.forename: W
authors.initials: W
authors.lastname: Köhler
authors.forename: T
authors.initials: T
authors.lastname: Wadström
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000378:metabolism; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005921:Glomerulonephritis / Q000382:microbiology; D006023:Glycoproteins / Q000378:metabolism / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000378:metabolism* / Q000494:pharmacology; D006497:Heparan Sulfate / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D011869:Radioligand Assay; D013297:Streptococcus pyogenes / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D006023:Glycoproteins; D006025:Glycosaminoglycans; D006497:Heparan Sulfate