[The determination of mycobacterial antigens found in immune complexes].
By using affine isolation from hyperimmune rabbit sera on homologous mycobacteria humanus (H37Rv) and affine purification on atypical mycobacteria and BCG mycobacteria, the authors obtained a preparation of antituberculous antibodies. These were used in indirect ELISA to detect antigens (after IC dissociation) in tuberculous patients. Out of 70 patients with infiltrative pulmonary tuberculosis, 30 patients with other pulmonary diseases and 30 healthy donors the antigens were found in 77.1% of tuberculous cases and in 6.7% of healthy subjects. The results of the test say in favour of its benefits in practical application. The antigens levels and incidence of positive reaction are higher in patients with destructive tuberculosis and in the bacterial discharge.
Baenskiĭ
A V
AV
Dem'ianenko
N V
NV
Kalinina
O A
OA
Sergeeva
L V
LV
Litvinov
V I
VI
D000907:Antibodies, Bacterial / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000097:blood*; D000942:Antigens, Bacterial / Q000097:blood*; D001991:Bronchitis / Q000175:diagnosis; D002846:Chromatography, Affinity; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial
Opredelenie antigenov mikobakteriĭ, nakhodiashchikhsia v immunnykh kompleksakh.