[The isolation and characteristics of monoclonal antibodies to recombinant proteins of HIV-1 and HIV-2--the env and gag gene products].
Ten hybridomas secreting monoclonal antibodies (Mab) against recombinant HIV-1 and HIV-2 antigens were produced (3 Mab anti-gag protein, 2 anti-env1, and 5 anti-env2). In the immunoblotting assay all the anti-gag Mabs reacted with HIV capsid protein p24, whereas one of them reacted also with p55 protein and with 7 other polypeptides. Another anti-gag Mab cross-reacted with the antigen of subpopulation of human peripheral blood lymphocytes. The third one interacted with the antigen of both HIV-1 and HIV-2. All the 10 Mabs interacted with natural HIV antigens and can be used for identification and differentiation of HIV-1 and HIV-2.
Pugach
A V
AV
Zverev
V V
VV
Pille
E R
ER
Shukhmina
N R
NR
Mel'nikova
N L
NL
Nosik
D N
DN
Maliushova
V V
VV
Andzhaparidze
O G
OG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D015686:Gene Products, env / Q000276:immunology*; D015683:Gene Products, gag / Q000276:immunology*; D015488:HIV Antigens / Q000097:blood; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D015686:Gene Products, env; D015683:Gene Products, gag; D015488:HIV Antigens; D011994:Recombinant Proteins
Poluchenie i kharakteristika monoklonal'nykh antitel k rekombinantnym belkam VICh-1 i VICh-2--produktam genov env i gag.