A novel gene delivery system using EGF receptor-mediated endocytosis.
A monoclonal antibody to the human epidermal growth factor (EGF) receptor was conjugated with polylysine and the resulting conjugate was affinity-linked with DNA (gene). This novel gene delivery system utilizes receptor-mediated endocytosis and would be especially suitable for gene therapy for EGF receptor-overproducing squamous cell carcinomas.
Chen
J
J
Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.
Gamou
S
S
Takayanagi
A
A
Shimizu
N
N
D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004247:DNA / Q000008:administration & dosage / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004705:Endocytosis; D066246:ErbB Receptors / Q000502:physiology*; D018014:Gene Transfer Techniques; D006801:Humans; D011107:Polylysine; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D011107:Polylysine; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D066246:ErbB Receptors