Conformation of polyene antibiotic, filipin III: CD and 1H NMR studies.
Detailed studies on the solution conformation of polyene antibiotic, filipin III using circular dichroism (CD) and proton nuclear magnetic resonance techniques have been carried out. In dimethyl sulfoxide (DMSO), filipin III exhibits concentration dependent aggregation-monomeric at lower and oligomeric at higher concentrations of the antibiotic. At concentrations used for 1H NMR studies (6 x 10(-3)M) the molecule coexists as monomeric and oligomeric species. However, titration experiments indicated that, in a mixed solvent system of DMSO:methanol (2:3 v/v) the antibiotic exists only as a monomer. Complete 1H NMR assignments and the conformation of the monomer filipin III have been determined by the combined use of DQFCOSY and ROESY experiments in DMSO:methanol solvent system.
Balakrishnan
A R
AR
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
Easwaran
K R
KR
D002942:Circular Dichroism; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005372:Filipin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure
D005372:Filipin; D000432:Methanol; D004121:Dimethyl Sulfoxide