[Oxidative dehalogenation of 2-chloro- and 2,4-dichlorobenzoates by Pseudomonas aeruginosa].
The strain Pseudomonas aeruginosa 142 isolated from the utilising PSBs bacterial association was capable of growth on 2-chloro- and 2,4-dichlorobenzoates as sole carbon sources, but it did not utilize 3-Cl, 4-Cl, 3,5-diCl- and 2,6-dichlorobenzoates. P. aeruginosa 142 dehalogenated 2-Cl-, 2,4-diCl- and 2,5-dichlorobenzoates in aerobic conditions. The release of chloride was not observed in microaerophilic and anaerobic conditions. The activities of catechol-1,2-dioxygenase and 4-chlorocatechol-1,2-dioxygenase were found in cell extracts after growth of this strain on 2,4-dichlorobenzoate. The presented results suggested that oxidative release of chloride in ortho-position is the first step of metabolism of 2-Cl-, 2,4-diCl- and 2,5-dichlorobenzoates. The further splitting of corresponding catechols is carried out by ortho-pathway.
Romanov
V L
VL
Grechkina
G M
GM
Adanin
V M
VM
Starovoĭtov
I I
II
D000332:Aerobiosis / Q000502:physiology; D050548:Catechol 1,2-Dioxygenase; D002396:Catechols / Q000737:chemistry; D002713:Chlorine / Q000378:metabolism*; D002723:Chlorobenzoates / Q000378:metabolism*; D049308:Dioxygenases; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
D002396:Catechols; D002723:Chlorobenzoates; D002713:Chlorine; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; C073069:2,5-dichlorobenzoic acid
Okislitel'noe degalogenirovanie 2-khlor- i 2,4-dikhlorbenzoatov shtammov Pseudomonas aeroginosa.