title: Age dependency of mitochondrial DNA decrease differs in different tissues of rat.
abstract: Mitochondrial DNA contents were measured using non-radioactive DNA probes for specific mtDNA sequences. The nuclear repetitive DNA sequence, LINE, was used as an internal standard marker. Age-dependent decreases in the mtDNA/nucDNA ratio in rat hepatic cells were confirmed with this method and, furthermore, a similar decrease was found in cardiac muscles. On the other hand, in thigh skeletal muscles such a decrease was not observed, slight increases being rather found in 18- to 24-month-old rats. The possible relationship of the decrease in mtDNA and the mechanism underlying animal senescence was discussed.
authors.affiliation: College of Medical Technology, Kyoto University.
authors.forename: K
authors.initials: K
authors.lastname: Asano
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Tauchi
authors.forename: A
authors.initials: A
authors.lastname: Asano
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D004272:DNA, Mitochondrial / Q000378:metabolism*; D005819:Genetic Markers; D008115:Liver Regeneration / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D015342:DNA Probes; D004272:DNA, Mitochondrial; D005819:Genetic Markers