title: Kinetic fluorimetric determination of promethazine by a stopped-flow mixing technique.
abstract: The oxidation of promethazine to its corresponding fluorescent sulphoxide was used to develop a novel kinetic fluorimetric method for the determination of this drug. The use of a stopped-flow mixing technique makes use of an oxidizing reagent unnecessary because the oxidation is rapidly carried out by dissolved oxygen. The method is simple and fast as it only requires a few seconds to obtain kinetic data which allows ready application to routine analyses. The calibration graph is linear over the range 0.5-80 micrograms ml-1 and the precision (%RSD) is close to 2%. The method was applied to the determination of promethazine hydrochloride in two pharmaceutical preparations.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
authors.forename: L
authors.initials: L
authors.lastname: de la Peña
authors.forename: A
authors.initials: A
authors.lastname: Gómez-Hens
authors.forename: D
authors.initials: D
authors.lastname: Pérez-Bendito
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D011398:Promethazine / Q000032:analysis*; D012996:Solutions / Q000737:chemistry; D013607:Tablets / Q000737:chemistry
chemical_list: D012996:Solutions; D013607:Tablets; D011398:Promethazine