title: Cloning and identification of testis-specific transcription elongation factor S-II.
abstract: A new S-II cDNA clone was isolated from a rat testis library. This cDNA contained an open reading frame encoding 299 amino acid residues. The deduced amino- and carboxyl-terminal regions were very similar with those of Ehrlich cell S-II, which we reported previously, but the sequence of the intervening 46 amino acid residues was unique. This new S-II was expressed in the testis but not in the other rat tissues examined, suggesting that it was a testis-specific S-II. Recombinant testis-specific S-II produced in Escherichia coli was shown to stimulate RNA polymerase II.
authors.affiliation: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors.forename: Q
authors.initials: Q
authors.lastname: Xu
authors.forename: T
authors.initials: T
authors.lastname: Nakanishi
authors.forename: K
authors.initials: K
authors.lastname: Sekimizu
authors.forename: S
authors.initials: S
authors.lastname: Natori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II