Ageing erythrocytes and alloxan diabetes: I. A possible role of catalase, GSH, GSSG, and GSH-enzymes in decreasing defence system.
The erythrocytes from control (C), diabetic (D) and insulin-treated diabetic (D+I) rats were separated into three ageing groups (TAG) i.e., light dense (young cells), intermediate-dense (middle-aged cells) and heavy-dense (old aged cells) samples. The activities of enzymes and metabolites changed from young to old cells in the following manner: (1) Increase of CAT in TAG and a lower level in D and D+I (2) Decrease of GPx in TAG but a low level in D (3) Increase of GR in TAG but a higher level in D, (4) Increase of GST in C and a decrease in D with a higher level in young cells and a lower level in middle-aged and old cells. The reversal of enzyme was more in young cells of D+I (5) Increase of GSH in TAG, a low level in D and a high level in D+I (6) Increase of GSSG in TAG, a high level found only in young cells of D. The results show that young red cells are affected more significantly in diabetes than other age cell types.
Gupta
B L
BL
Hormone and Drug Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Ansari
M A
MA
Srivastava
P
P
Baquer
N Z
NZ
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002374:Catalase / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000201:enzymology; D004905:Erythrocyte Aging / Q000502:physiology*; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005978:Glutathione / Q000097:blood*; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D006454:Hemoglobins / Q000032:analysis; D007328:Insulin / Q000494:pharmacology; D051381:Rats
D001786:Blood Glucose; D006454:Hemoglobins; D007328:Insulin; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione