title: [A healthy carrier of verotoxin-producing Escherichia coli, who was detected by periodic feces examinations].
abstract: Verotoxin-producing Escherichia coli (VTEC) was rapidly detected by the PCR method in one of the 9 feces samples. They were collected from the people who had been cooking meals for patients on a periodic feces examination on November 25, 1992. It was confirmed by PCR that the isolate had both VT1 and VT2vh toxic genes and showed cytotoxicity on Verocells. In this case, the isolate was highly susceptible to common antibiotic agents, it was removed by the administration of tosfulaxacin. Some isolates which had the same properties as those of the strain described in "VITEK GNI card" and the antimicrobial susceptibility and VT toxin types, were detected in 2 of the 3 members of her family, they were healthy carriers and did not have any subjective symptoms such as diarrhea or abdominal pain. In order to detect Verotoxic genes, the sample of feces preincubated for 3 h in trypticase broth was subjected to PCR. We recommend that this method is much more useful because of rapid detection and identification of VTEC compared with the classical culture method.
authors.affiliation: Shionogi Biomedical Laboratories, Shionogi Pharmaceutical Co., Ltd.
authors.forename: Y
authors.initials: Y
authors.lastname: Murata
authors.forename: S
authors.initials: S
authors.lastname: Kusunoki
authors.forename: W
authors.initials: W
authors.lastname: Minamide
authors.forename: H
authors.initials: H
authors.lastname: Igimi
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: T
authors.initials: T
authors.lastname: Takeda
mesh_terms: D000328:Adult; D000368:Aged; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002353:Carrier State / Q000382:microbiology*; D002648:Child; D004926:Escherichia coli / Q000302:isolation & purification / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D022622:Shiga Toxin 1
chemical_list: D001427:Bacterial Toxins; D022622:Shiga Toxin 1