title: The influence of histatin-5 fragments on the mineralization of hydroxyapatite.
abstract: The adsorption of histatin 5 on hydroxyapatite (HAP) was determined and compared to that of several fragments of histatin 5, such as residues 1-16 (N16), 7-16 (M10), 9-24 (C16), 11-24 (C14), 13-24 (C12), 15-24 (C10). The influence of the adsorbed peptides on the seeded crystal growth of HAP was investigated with the constant composition method. The adsorption affinity of the peptides as well as their ability to inhibit mineralization was influenced by the length of the peptide chain. Histatin 5 showed the highest affinity, as determined by a Langmuir model, whereas the smaller C10 and C12 displayed the lowest equilibrium uptake. The smaller C10 and C12 peptides were, on the other hand, more effective as crystal growth inhibitors, indicating a more efficient coverage of surface active sites. Electrophoretic mobility data indicated an increase in the positive charge at the HAP surface in the presence of these peptides, which were efficient HAP crystallite dispersants.
authors.affiliation: Department of Chemistry, State University of New York at Buffalo 14214.
authors.forename: C F
authors.initials: CF
authors.lastname: Richardson
authors.forename: M
authors.initials: M
authors.lastname: Johnsson
authors.forename: P A
authors.initials: PA
authors.lastname: Raj
authors.forename: M J
authors.initials: MJ
authors.lastname: Levine
authors.forename: G H
authors.initials: GH
authors.lastname: Nancollas
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry* / Q000378:metabolism*; D004586:Electrophoresis; D054808:Histatins; D006801:Humans; D008969:Molecular Sequence Data; D010316:Particle Size; D010446:Peptide Fragments / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
chemical_list: C068075:HTN3 protein, human; D054808:Histatins; D010446:Peptide Fragments; D012471:Salivary Proteins and Peptides; D017886:Durapatite