Islet-specific T-cell clones from the NOD mouse respond to beta-granule antigen.
Islet-reactive T-cell clones from NOD mice provide an important approach to the investigation of antigens with relevance to type I diabetes. To identify a source of beta-cell antigen suitable for biochemical studies, we have used two islet-specific, diabetogenic T-cell clones to test beta-tumor cells. beta-tumor cell lines, maintained in continuous culture, were found to lose antigenicity rapidly. However, cells harvested directly from beta-tumors arising spontaneously in the transgenic NOD/Lt-Tg(RIPTag)1Lt mouse proved to be a potent source of beta-cell antigen for the T-cell clones. Subcellular fractionation of beta-tumor cells showed that the T-cell antigen was highly enriched in the beta-granule fraction and that this activity was associated with the granule membrane.
Bergman
B
B
Barbara Davis Center for Childhood Diabetes, Denver, Colorado 80262.
Haskins
K
K
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods; D003594:Cytoplasmic Granules / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007340:Insulinoma / Q000276:immunology*; D007515:Islets of Langerhans / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D016688:Mice, Inbred NOD / Q000276:immunology; D008815:Mice, Inbred Strains / Q000276:immunology; D010190:Pancreatic Neoplasms / Q000276:immunology*; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm