Fibrinogen as a ferritin-binding protein in horse plasma.
Lower apparent concentrations of ferritin were observed in horse plasma than in serum using the enzyme-linked immunosorbent assay (ELISA). However, the ferritin concentrations in plasma and serum were increased to the same level on heating the samples at 75 degrees C for 15 min. These results suggest that horse plasma has specific ferritin-binding protein(s) which inhibit(s) the ferritin assay. The apparent ferritin concentrations in horse serum were markedly decreased by adding horse fibrinogen to the serum. It was also found that fibrinogen bound to spleen ferritin and inhibited the immunoassay of this protein. From these results, it was concluded that horse fibrinogen is one of the ferritin-binding proteins which inhibit the immunoassay of horse ferritin.
Orino
K
K
Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Yamamoto
S
S
Watanabe
K
K
D000818:Animals; D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay; D005293:Ferritins / Q000097:blood*; D005340:Fibrinogen / Q000378:metabolism*; D006736:Horses / Q000097:blood*; D007700:Kinetics; D011485:Protein Binding; D013154:Spleen / Q000378:metabolism
D000906:Antibodies; D005340:Fibrinogen; D005293:Ferritins