Cloning and sequence analysis of a cDNA encoding rice glutaredoxin.
A full-length cDNA clone (RASC8) encoding glutaredoxin (thioltransferase) was isolated from a cDNA library of an aleurone layer prepared from a developing seed of rice (Oryza sativa L.). RASC8, 568bp in length, contained an ATG codon and two possible polyadenylation signals, and encoded 112 amino acid residues. Cys-Pro-Phe-Cys, which is the active site and a highly conserved sequence among thioltransferases, was found in the deduced amino acid sequence. RASC8 was introduced into an expression vector pMALc2 and the translated product possessed thioltransferase activity.
Minakuchi
K
K
Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, Japan.
Yabushita
T
T
Masumura
T
T
Ichihara
K
K
Tanaka
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000096:biosynthesis*; D015723:Gene Library; D017343:Genes, Plant; D054477:Glutaredoxins; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D011490:Protein Disulfide Reductase (Glutathione); D011506:Proteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D013552:Swine
D018076:DNA, Complementary; D054477:Glutaredoxins; D010940:Plant Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases; D011490:Protein Disulfide Reductase (Glutathione)