Interphase cytogenetics and flow cytometry analyses of renal tumours.
Fluorescent in situ hybridization (FISH) can be used to determine chromosome changes in human neoplasia. In our study, we have tested the feasibility of FISH to interphase cells of renal carcinoma to evaluate chromosome aneuploidies. We carried out in parallel in situ hybridization and flow cytometric studies in order to evaluate the possible correlation between numerical chromosome abnormalities and ploidies detected by flow cytometry (FCM). The ploidy of chromosomes 7, 11, 17 and 18 was investigated in three cases of this tumour utilizing specific probes. We found evaluable and comparable results in every case of renal carcinoma analyzed for both FISH and DNA FCM analyses and our results indicate that fluorescent in situ hybridization with chromosome-specific repetitive DNA probes can serve as a cytogenetic tool for the detection of numerical specific chromosome abnormalities of interphase nuclei of renal carcinoma.
Grollino
M G
MG
Environmental Biomedicine Division, ENEA Casaccia, Rome, Italy.
Cavallo
D
D
Di Silverio
F
F
Rocchi
M
M
De Vita
R
R
D000782:Aneuploidy; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D002880:Chromosomes, Human, Pair 11; D002886:Chromosomes, Human, Pair 17; D002887:Chromosomes, Human, Pair 18; D002897:Chromosomes, Human, Pair 7; D015342:DNA Probes; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D011003:Ploidies
D015342:DNA Probes