Membrane-bound 5'-nucleotidase/nucleoside phosphotransferase from Bacillus cereus.
1. A search for nucleoside phosphotransferase activity in Bacillus cereus led to the following results: (i) The phosphotransferase activity was associated with a membrane bound 5'-nucleotidase. (ii) The enzyme phosphorylates both purine and pyrimidine nucleosides as well as 2',3'-dideoxyinosine. (iii) The enzyme was inhibited by adenylic nucleotide di- and triphosphates, and its nucleotidase activity was increased in the presence of inosine as phosphate acceptor. 2. Bacterial and vertebrate 5'-nucleotidases with phosphotransferase activity differ for several characteristics, such as cellular location, substrate specificity, magnesium requirement and regulation.
Baiocchi
C
C
Dipartimento di Fisiologia e Biochimica, Università di Pisa, Italy.
Pesi
R
R
Turriani
M
M
Tozzi
M G
MG
Camici
M
M
Ipata
P L
PL
D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D001409:Bacillus cereus / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; C021892:nucleoside phosphotransferase; D015720:5'-Nucleotidase