title: Crystallization of 31 kDa C-terminal fragment of rat DNA polymerase beta.
abstract: A 248 residue C-terminal fragment of rat DNA polymerase beta (335 amino acid residues), a eukaryotic DNA repair enzyme, has been crystallized from polyethylene glycol 6000 solution. The crystals are orthorhombic, space group P2(1)2(1)2 with cell dimensions a = 120.3 A, b = 64.2 A, c = 39.4 A, and contain a single 31 kDa fragment in an asymmetric unit. The crystals diffract to 2.8 A resolution with laboratory X-ray source, and to 2.3 A resolution with synchrotron X-ray source, and are suitable for detailed structural analysis.
authors.affiliation: Department of Physics, Hyogo University of Education, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shirakihara
authors.forename: A
authors.initials: A
authors.lastname: Matsukage
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimoto
authors.forename: T
authors.initials: T
authors.lastname: Date
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D004256:DNA Polymerase I / Q000737:chemistry*; D051381:Rats; D014357:Trypsin
chemical_list: D004256:DNA Polymerase I; D014357:Trypsin