Low-temperature scanning electron microscopy in fungus-nematode interaction.
A comparative study has been made of different preparation techniques used for the scanning electron microscope (SEM), with regard to their application to fungus-nematode interaction. The preparation of frozen-hydrated specimens of both healthy and Arthrobotrys-oligospora-infected second-stage larvae of the root-knot nematode (Meloidogyne sp.) is described, and the results are compared with those obtained by critical point-drying and freeze-drying. In all cases the frozen-hydrated specimens consistently showed the best preservation.
den Belder
E
E
Department of Biological and Integrated Control, DLO Research Institute for Plant Protection (IPO-DLO), Wageningen, The Netherlands.
Boekestein
A
A
van Esch
J W
JW
Thiel
F
F
D000818:Animals; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D003904:Mitosporic Fungi / Q000502:physiology / Q000648:ultrastructure*; D013696:Temperature; D014431:Tylenchoidea / Q000502:physiology / Q000648:ultrastructure*