title: Human secretory immunoglobulin A anti-Entamoeba histolytica antibodies inhibit adherence of amebae to MDCK cells.
abstract: The presence of secretory immunoglobulin A (IgA) anti-Entamoeba histolytica antibodies in the saliva of patients with intestinal amebiasis was demonstrated by immunoblot assay, and the capacity of these antibodies to inhibit amebic adherence to a monolayer of MDCK cells was analyzed. Inhibition was due to IgA antiamebic antibodies and in part to anti-Gal-binding-lectin antibodies, as demonstrated by absorption experiments with total amebic extract and with the fraction of Gal-binding lectin. These results emphasize the relevance of secretory IgA antibodies in the phenomenon of E. histolytica adherence to epithelial cells.
authors.affiliation: Departmento de Inmunología, Universidad Nacional Autónoma de México, México, D.F.
authors.forename: J C
authors.initials: JC
authors.lastname: Carrero
authors.forename: M Y
authors.initials: MY
authors.lastname: Díaz
authors.forename: M
authors.initials: M
authors.lastname: Viveros
authors.forename: B
authors.initials: B
authors.lastname: Espinoza
authors.forename: E
authors.initials: E
authors.lastname: Acosta
authors.forename: L
authors.initials: L
authors.lastname: Ortiz-Ortiz
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000494:pharmacology*; D000953:Antigens, Protozoan / Q000302:isolation & purification; D002448:Cell Adhesion / Q000276:immunology; D002460:Cell Line; D004285:Dogs; D004404:Dysentery, Amebic / Q000276:immunology; D004748:Entamoeba histolytica / Q000276:immunology* / Q000502:physiology; D037161:Galectins; D006388:Hemagglutinins / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000276:immunology / Q000302:isolation & purification; D012463:Saliva / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D037161:Galectins; D006388:Hemagglutinins; D007071:Immunoglobulin A, Secretory; D015800:Protozoan Proteins