A panel of deleted mouse X chromosome somatic cell hybrids derived from the embryonic stem cell line HD3 shows preferential breakage in the Hprt-DXHX254E region.
A panel of 91 somatic cell hybrids containing deleted mouse X chromosomes and falling into seven nested intervals has been isolated and characterized from fusions involving the murine embryonic stem cell HD3. Many of the X chromosome breakpoints present in these hybrids fall within regions in which few or no other hybrids were previously available. The apparent enrichment for breakpoints lying within the Hprt-DXHX254E region is discussed in relation to both the nature of the embryonic stem cell fusions and the presence of the Fmr1 gene associated with FRAXA in man within this span.
Arnaud
D
D
Unité de Génétique Moléculaire Murine, Institut Pasteur, Paris, France.
Mattei
M G
MG
Avner
P
P
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D005260:Female; D051860:Fragile X Messenger Ribonucleoprotein 1; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007621:Karyotyping; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D016601:RNA-Binding Proteins / Q000235:genetics; D013234:Stem Cells; D014960:X Chromosome
D017931:DNA Primers; D051860:Fragile X Messenger Ribonucleoprotein 1; D005819:Genetic Markers; D007041:Hypoxanthine Phosphoribosyltransferase; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C497683:FMR1 protein, human; C497684:Fmr1 protein, mouse