title: Increased longevity of EF-1 alpha high-fidelity mutants in Podospora anserina.
abstract: Various translation initiation and elongation factors seem to participate in the control of the cellular proliferation and the ageing process in higher eukaryotes. Studies indicate that EF-1 alpha, one of the translation elongation factors, may be one of the major components involved. We here present the cloning of the filamentous fungus P. anserina EF-1 alpha encoding gene and show that strains bearing high fidelity mutations in the EF-1 alpha gene have a drastically increased longevity as well as an impairment in sporulation. This suggests that EF-1 alpha involved in the sexual and senescence processes in lower eukaryotes, through the control of translational errors.
authors.affiliation: Centre de Génétique Moléculaire, CNRS, Gif sur Yvette, France.
authors.forename: P
authors.initials: P
authors.lastname: Silar
authors.forename: M
authors.initials: M
authors.lastname: Picard
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D013098:Xylariales / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors