Sequence of the 3'-noncoding region of the luteinizing hormone receptor gene and identification of two polyadenylation domains that generate the major mRNA forms.
We present 6.2 kb of the 3'-noncoding region sequence of the rat luteinizing hormone receptor (LHR) gene and identification of two functional polyadenylation (pA) domains, H1 (nt 2368-2491) and H2 (nt 5579-5768) responsible for 3'-end processing of the 2.6/2.3 kb and the 5.8 kb LHR mRNA, respectively. Two identical copies of pA elements AAUAUA in H1 and of AAUAAA in H2 account for micro-heterogeneous poly(A) addition at each of the two pA regions. Both LH holoreceptor and major splice variant form B (lacking the first 266 bp of exon 11) are identified in H1-terminated (2.6 kb and 2.3 kb) and H2-terminated (5.8 kb) mRNA transcripts. A rodent repetitive DNA LINE R domain 3' of H1 within the major 5.8 kb species and a B2 element downstream of H2 were identified. Alignment of the 3'-noncoding region of LHR with TSH, FSH and beta 2-adrenergic receptors indicate that H1 pA signal is unique to the LHR and may represent an insertion domain.
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Section on Molecular Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Buczko
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Zhuang
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Dufau
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D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D011061:Poly A; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D018343:Receptors, Adrenergic, beta-2; D011974:Receptors, LH; D011061:Poly A