Use of 10-propargyl-5,8-dideazafolate and directed mutagenesis to probe the catalytic mechanism of thymidylate synthase.
Spencer
H T
HT
Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, TN 38101.
Villafranca
J E
JE
Appleman
J R
JR
D000595:Amino Acid Sequence; D001665:Binding Sites; D003545:Cysteine; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005492:Folic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D005493:Folic Acid Antagonists / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D016296:Mutagenesis; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013940:Thymidylate Synthase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D005493:Folic Acid Antagonists; D011994:Recombinant Proteins; C072406:10-propargyl-5-deazafolic acid; D005492:Folic Acid; D013940:Thymidylate Synthase; D003545:Cysteine