Synthesis of deacetyl-thymosin beta 12 and examination of its immunological effects on the impaired T and B lymphocytes in uremic patients.
Deacetyl-thymosin beta 12 was synthesized in a conventional manner by assembling six peptide fragments followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole (molar ratio, 1:1) in trifluoroacetic acid in the presence of m-cresol and dimethyl-selenium. Incubation of peripheral lymphocytes isolated from uremic patients with the synthetic deacetyl-thymosin beta 12 showed an enhancing effect on the reduced beta lymphocytes but had no restoring effect on the impaired blastogenic response of T lymphocytes.
Abiko
T
T
Kidney Research Laboratory, Sendai, Japan.
Sekino
H
H
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013947:Thymosin / Q000138:chemical synthesis* / Q000494:pharmacology; D014511:Uremia / Q000276:immunology*
C085519:deacetylthymosin beta(12); D013947:Thymosin