Physical characterization of plasmid pMQV10 from a steroid biotransforming strain of Micrococcus.
The presence of a single plasmid 8.5 kb in size has been demonstrated in a cholesterol biotransforming strain of Micrococcus. A detailed physical map of the plasmid has been constructed using various restriction enzymes. Streptomycin resistance has been localized on a 1.8-kb fragment of pMQV10.
Verma
V
V
Genetic Engineering Unit, C.S.I.R., Jammu-Tawi, India.
Felder
M
M
Redenbach
M
M
Qazi
G N
GN
Cullum
J
J
D001711:Biotransformation; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D008837:Micrococcus / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D013256:Steroids / Q000378:metabolism*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013256:Steroids