Nystatin-perforated patch recordings disclose KA-operated outward currents in rat cortical neurons.
Kainate (KA)-induced responses were studied in acutely dissociated rat cortical neurons. KA elicited the inward currents with conventional whole-cell and nystatin-perforated patch recordings under voltage-clamp condition. An additional outward current was observed only with the perforated patch recording. The outward current was due to the activation of K+ current by Ca2+ passing through the KA channels. This K+ channel was sensitive to both iberiotoxin and tetraethylammonium (TEA)-Cl. This K+ channel may serve to limit the depolarization during excessive KA-mediated excitation.
Omura
T
T
Department of Neurophysiology, School of Medicine, Tohoku University, Sendai, Japan.
Munakata
M
M
Akaike
N
N
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects*; D005071:Evoked Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D009761:Nystatin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018092:Kainic Acid Receptors / Q000187:drug effects*
D009761:Nystatin; D018092:Kainic Acid Receptors