Loss of glomerular anionic sites and the development of albuminuria in rats with streptozotocin-induced diabetes.
Examination was made of changes in the anionic sites of the glomerular basement membrane (GBM) in rats with streptozotocin (STZ)-induced diabetes by the immersion method of polyethyleneimine (PEI). PEI particles in GBM of diabetic rats significantly decreased from the 1st through the 8th week. Urinary albumin excretion in diabetic rats significantly increased at the 2nd but not earlier week. Insulin treatment effectively prevented decrease in PEI particles in STZ-injected rats. In rats with STZ-induced diabetes, initial renal alteration was disturbance of the charge barrier, followed by the development of albuminuria. Continued deterioration of anionic sites and possibly additional disturbance of size barrier were considered responsible for the development of albuminuria. Insulin treatment appears to prevent the loss of anionic sites of GBM.
Moriya
T
T
Department of Internal Medicine, Kitasato University School of Medicine, Sagamihara, Japan.
Nakazawa
K
K
Itoh
N
N
Shigematsu
H
H
Okada
N
N
Aizawa
T
T
Yamada
T
T
Yajima
Y
Y
D000419:Albuminuria / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000838:Anions; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D001665:Binding Sites; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D015335:Molecular Probes; D011094:Polyethyleneimine / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000838:Anions; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D015335:Molecular Probes; D011094:Polyethyleneimine; D011509:Proteoglycans