Two related, low-temperature-induced genes from Brassica napus are homologous to the human tumour bbc1 (breast basic conserved) gene.
In order to identify genes involved in cold acclimation, we have constructed a cDNA library from Brassica napus (cv. Samouraï) cold-acclimated etiolated seedlings. By differential screening, a cDNA clone named pBnC24 (Brassica napus Cold), corresponding to a new cold-inducible plant gene, was isolated. Northern blot hybridizations using total RNA from acclimated and unacclimated seedlings confirmed that BnC24 represents a cold-regulated gene. In contrast with a number of cold-inducible plant genes, BnC24 does not seem to be responsive to abscisic acid (ABA). In addition, further screening of the 'cold-acclimated' cDNA library using pBnC24 cDNA as a probe, allowed the isolation of a second type of homologous cDNA. Sequence analysis showed that the two BnC24 genes encode basic 24 kDa proteins, which are highly hydrophilic and rich in alanine, lysine and arginine. The nucleotide and deduced amino acid sequences of these clones do not show any homology with other previously described cold-induced plants genes. However they have strong homology with a recently discovered human tumour gene, bbcl (breast basic conserved), which seems to be highly conserved in eukaryotes.
Sáez-Vásquez
J
J
Laboratoire de Physiologie et Biologie Moléculaire Végétales, URA 565 du CNRS, Université de Perpignan, France.
Raynal
M
M
Meza-Basso
L
L
Delseny
M
M
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D015870:Gene Expression / Q000187:drug effects; D005796:Genes; D017343:Genes, Plant; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009994:Osmolar Concentration; D010940:Plant Proteins / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012269:Ribosomal Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
C085489:BnC24 protein, Brassica napus; D009363:Neoplasm Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C085488:RPL13 protein, human; D012269:Ribosomal Proteins; D000040:Abscisic Acid