Determination of erythrocyte aldehyde dehydrogenase activity by high-performance liquid chromatography.
An ion-paired high-performance liquid chromatographic method was developed for the determination of erythrocyte aldehyde dehydrogenase activity. This method does not require pretreatment to remove hemoglobin from the hemolysate before enzyme reaction is initiated. In addition, the advantage with this high-performance liquid chromatographic method is that erythrocyte aldehyde dehydrogenase activity can be measured using whole blood without either separation or washing of erythrocytes. Therefore, it can be easily used in the determination of erythrocyte aldehyde dehydrogenase activity, which is an indication of excess alcohol consumption.
Yamamoto
T
T
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
Moriwaki
Y
Y
Takahashi
S
S
Nasako
Y
Y
Hiroishi
K
K
Higashino
K
K
D000079:Acetaldehyde / Q000097:blood; D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000097:blood; D000444:Aldehyde Dehydrogenase / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D000431:Ethanol / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009243:NAD / Q000097:blood; D012016:Reference Values
D009243:NAD; D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde