Characteristic mRNA abnormality found in half the patients with severe haemophilia A is due to large DNA inversions.
Surprisingly half of all severe haemophilia A patients have no mutation in the promoter, coding sequences and normal RNA processing signals of the factor VIII gene. Instead they manifest a unique mRNA defect that prevents the amplification of the message across the boundary between exon 22 and 23. This locates the defect to internal regions of intron 22. Novel sequences 3' to exon 22 were isolated from the 9 available patients with the above abnormality by combining RACE and vectorette amplifications on trace amounts of mRNA. This showed that exons 1-22 of the factor VIII mRNA had become part of a hybrid message containing new multi exonic sequences expressed in normal cells. The novel sequences were not located in a YAC covering the whole factor VIII gene. Southern blots from patients probed by novel sequences and clones covering intron 22 showed no obvious abnormalities. This suggested inversions involving intron 22 repeated sequences. Screening of 3 YAC libraries with the novel sequences located them at least 200 kb telomeric (5') to factor VIII and pulsed field gel analysis detected abnormal bands in patients. This demonstrates that the mutations in the patients are inversions of long DNA regions possibly involving the repeated sequences and occurring at the surprising rate of approximately 4 x 10(-6) per gene per gamete per generation.
Naylor
J
J
Division of Medical and Molecular Genetics, United Medical School, Guy's Hospital, London, UK.
Brinke
A
A
Hassock
S
S
Green
P M
PM
Giannelli
F
F
D001483:Base Sequence; D007446:Chromosome Inversion; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D005091:Exons; D005169:Factor VIII / Q000235:genetics*; D005784:Gene Amplification; D006467:Hemophilia A / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping
D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D005169:Factor VIII; D004247:DNA