title: Phenotypic mapping of the chicken embryonic thymic microenvironment developing within an organ culture system.
abstract: The chicken thymic microenvironment, as it developed in an embryonic thymus organ culture system, was phenotypically mapped using a panel of mAb defining both epithelial and nonepithelial stromal cell antigens. We have previously reported that thymocyte proliferation and differentiation with proceed for up to 6-8 days in thymus organ culture, hence demonstrating the functional integrity of the thymic microenvironment in vitro. During this time, the stromal component reflected that of the normal embryo with cortical and medullary epithelial areas readily identifiable by both morphology and surface-antigen expression. An abundance of subcapsular and cortical epithelial antigens was detected in the cultured thymus, particularly those normally expressed by the epithelium lining the capsule, trabeculae, and vascular regions (type I epithelium) in the adult and embryonic thymus. Medullary epithelial antigens developed in organ culture, although were present in lower frequency than observed in the age-matched embryonic thymus. MHC class II expression by both epithelial and nonepithelial cells was maintained at high levels throughout the culture period. With increasing time in culture, the ratio of epithelial to nonepithelial cells decreased, concurrent with a decrease in thymocyte frequency and suggestive of a bidirectional interaction between these two cell types. Thus, a functionally intact thymic microenvironment appears to be maintained in embryonic thymus organ culture, a model that is currently being exploited to assess the role of stromal antigens, as defined by our mAb, in the process of thymopoiesis.
authors.affiliation: Department of Pathology and Immunology, Monash University Medical School, Prahran, Australia.
authors.forename: N J
authors.initials: NJ
authors.lastname: Davidson
authors.forename: R L
authors.initials: RL
authors.lastname: Boyd
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000302:isolation & purification*; D002642:Chick Embryo; D004848:Epithelium / Q000196:embryology / Q000276:immunology; D005455:Fluorescent Antibody Technique; D009924:Organ Culture Techniques; D013950:Thymus Gland / Q000033:anatomy & histology / Q000196:embryology* / Q000276:immunology*; D014018:Tissue Distribution
chemical_list: D000943:Antigens, Differentiation