[3H]7-OH-DPAT labels both dopamine D3 receptors and sigma sites in the bovine caudate nucleus.
The binding of [3H]7-hydroxy-N,N-di-n-propyl-2-aminotetralin (7-OH-DPAT) to membranes of the bovine caudate nucleus was characterized and shown to be heterogenous. While [3H]7-OH-DPAT labels the dopamine D3 receptor in this tissue (Ki = 4.8 nM), as shown by its sensitivity to dopamine and (-)-quinpirole, it also binds with high affinity (Ki = 48 nM) to sigma recognition sites. The present results demonstrate that [3H]7-OH-DPAT is a useful radioligand to label native D3 receptors in the bovine caudate, although care must be taken to assure its selectivity.
Schoemaker
H
H
Department of Preclinical Research, Synthélabo Recherche, Bagneux, France.
D000818:Animals; D002417:Cattle; D002421:Caudate Nucleus / Q000378:metabolism*; D011869:Radioligand Assay; D011954:Receptors, Dopamine / Q000378:metabolism*; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D017480:Receptors, sigma / Q000378:metabolism*; D013764:Tetrahydronaphthalenes / Q000378:metabolism*; D014316:Tritium
D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D017480:Receptors, sigma; D013764:Tetrahydronaphthalenes; D014316:Tritium; C031349:7-hydroxy-2-N,N-dipropylaminotetralin