Determination of etoposide in blood by liquid chromatography with electrochemical detection.
A liquid chromatographic (LC) method for determination of etoposide in dog blood is described. The technique includes solvent extraction of etoposide using a dichloroethane-hexane mixture and reconstitution of the drug in an aqueous reconstitution solution. The samples are analyzed by reversed-phase LC with electrochemical detection. Validation of the method demonstrated good sensitivity, precision and reproducibility. The method is useful for the study of etoposide pharmacokinetics in the dog.
Eisenberg
E J
EJ
Department of Pharmaceutical Sciences, Sterling Winthrop, Inc., Malvern, PA 19355.
Eickhoff
W M
WM
D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D004563:Electrochemistry; D005047:Etoposide / Q000097:blood*; D015203:Reproducibility of Results
D005047:Etoposide