Effect of dimethyl sulfoxide pretreatment on activities of lipid peroxide formation, superoxide dismutase and glutathione peroxidase in the mouse liver after whole-body irradiation.
We investigated the effects of dimethyl sulfoxide (DMSO) on radiation damage in the mouse. DMSO (i.p. 0.11 g/mouse) administered 30 min before exposure protected the mice from the gamma-whole body irradiation: the 30 days lethality was significantly decreased from 44% to 16% (P < 0.05). The contents of thiobarbituric acid reactive substances(TBA-RS) in the mouse liver increased linearly between days 2 and 10 after 9 Gy gamma ray irradiation. The TBA-RS contents in the liver on days 2 to 10 after irradiation were reduced by DMSO pretreatment. The irradiation decreased superoxide dismutase (SOD) activity in the liver on day 10. Decrease in SOD activity was prevented by DMSO pretreatment. In the electron microscopic study, the mitochondria in the irradiated mouse liver were swollen, but we could observe no change after DMSO pretreatment. The results suggest that DMSO has radioprotective effects, probably due to inhibition of lipid peroxidation.
Ueda
T
T
Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan.
Toyoshima
Y
Y
Kushihashi
T
T
Hishida
T
T
Yasuhara
H
H
D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008054:Lipid Peroxides / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008933:Mitochondrial Swelling / Q000187:drug effects / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014916:Whole-Body Irradiation / Q000009:adverse effects*
D008054:Lipid Peroxides; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D004121:Dimethyl Sulfoxide