Separation of water-soluble vitamins via high-performance capillary electrophoresis.
A standard sample and method for performance evaluation in capillary zone electrophoresis is defined. The sample, containing thiamine, nicotinamide and nicotinic acid, was analysed in a 20 mM sodium phosphate buffer pH 7. The impact of pH, buffer type and ionic strength on electroosmotic flow, electrophoretic mobility, and peak shape was investigated. The separation of several water-soluble vitamins in phosphate buffer pH 7 was developed in order to analyse an over-the-counter vitamin preparation. Spectral analysis and peak purity tests were applied on-line for peak identification.
Jegle
U
U
Hewlett-Packard GmbH, Waldbronn Analytical Division, Germany.
D004586:Electrophoresis / Q000379:methods*; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D009525:Niacin / Q000302:isolation & purification; D009536:Niacinamide / Q000302:isolation & purification; D015203:Reproducibility of Results; D012995:Solubility; D013057:Spectrum Analysis; D013831:Thiamine / Q000302:isolation & purification; D014815:Vitamins / Q000302:isolation & purification*
D014815:Vitamins; D009536:Niacinamide; D009525:Niacin; D013831:Thiamine