Determination of peptide 520 in human plasma using post-column photolysis with electrochemical detection in liquid chromatography.
A simple LC method for the determination of peptide 520 in human plasma was developed. Based on micellar chromatography, sodium octyl sulphate (SOS) was added into the mobile phase in order to separate the peptide from human plasma components. The procedure was fast and sensitive for the determination of the peptide in untreated human plasma. The electrochemical (EC) detection limit for peptide 520 in human plasma was 0.5 microgram ml-1. Linearity of the calibration plot for peptide 520 in human plasma was 0.999. This approach represents a direct injection technique for the potential detection and analysis of numerous peptides in biofluids, besides just plasma, with absolute quantification.
Chen
L
L
Department of Chemistry, Northeastern University, Boston, MA 02115.
Mazzeo
J
J
Krull
I S
IS
Wu
S L
SL
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D010455:Peptides / Q000097:blood*; D010782:Photolysis
D010455:Peptides