Cell-cycle-stage specificity of the methotrexate block as resolved by X-ray-induced chromosome damage.
Radiation-induced chromosome aberrations were used as biomarkers to compare G0, mid-G1, and methotrexate (MTX)-arrested lymphocytes. The ratio of chromosome-type to chromatid-type aberrations in MTX-arrested cells was consistent with that predicted when postreplicative chromosomes are exposed to ionizing radiation and supports the premise that MTX arrests cells in late S/G2 of the cell cycle.
Richardson
V B
VB
Department of Biology, Tuskegee University, AL 36088.
Littlefield
L G
LG
Sayer
A M
AM
Peterson
P T
PT
D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D008727:Methotrexate / Q000494:pharmacology*
D008727:Methotrexate