Minute virus of mice-induced modification of the murine DNA polymerase alpha-primase complex permits the salt-induced dissociation of 12S DNA primase and 10S DNA polymerase alpha components.
DNA polymerase alpha-primase complexes in extracts of MVM-infected murine cells were dissociated in the presence of 0.3M KCl to generate a 12S DNA primase and a 10S DNA polymerase alpha that were readily separated by sedimentation in glycerol gradients. A 12S DNA polymerase alpha-primase complex refractory to dissociation in 0.3M KCl was identified in extracts of MVM-infected HeLa cells. In extracts of mock-infected murine and HeLa cells DNA primase and DNA polymerase alpha were not dissociated from each other in 0.3M KCl but remained in a stable complex that sedimented at 10S. We propose that a novel 12S DNA polymerase alpha-primase complex prone to disruption by salt is induced by MVM infection and that the DNA primase component of the complex is modified.
Gupta
J S
JS
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia.
Faust
E A
EA
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000502:physiology; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004257:DNA Polymerase II / Q000302:isolation & purification*; D019915:DNA Primase; D004279:DNA, Viral / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008915:Minute Virus of Mice / Q000201:enzymology*; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification* / Q000378:metabolism*; D012492:Salts
D004279:DNA, Viral; D012492:Salts; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004257:DNA Polymerase II