Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification.
We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed specimens. Based on comparative genomic hybridization and on universal DNA amplification procedures this technique is possible even if only a few tumor cells are available. We demonstrate the feasibility of this method to visualize complete and partial chromosome gains and losses and gene amplifications in archived solid tumor samples.
Speicher
M R
MR
Institut für Humangenetik, Universität Heidelberg, Germany.
du Manoir
S
S
Schröck
E
E
Holtgreve-Grez
H
H
Schoell
B
B
Lengauer
C
C
Cremer
T
T
Ried
T
T
D001483:Base Sequence; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D016650:Fluorescein-5-isothiocyanate; D005557:Formaldehyde; D015894:Genome, Human; D006652:Histological Techniques; D006801:Humans; D007621:Karyotyping; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D009693:Nucleic Acid Hybridization; D010232:Paraffin; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004273:DNA, Neoplasm; D005557:Formaldehyde; D010232:Paraffin; D016650:Fluorescein-5-isothiocyanate