[Study on 125I-labeled human chorinic gonadotropin after purification through gel electrophoresis].
Radioiodination of HCG with 125I Na using chloramine-T is widely employed. However, 125I-HCG damages in storage and thus results in decrease of immunological and biological activities. When 125I-HCG was in store as long as 30 days, the immunological activity and biological activity decreased from 100% to 33.1% and 44.0%, respectively. We used polyacrylamide gel electrophoresis to purify 125I-HCG which had been kept in store for 30 days since radiolabeling, and we found the binding capacities of purified 125I-HCG increased. The combination rates of purified 125I-HCG with antibody and receptors accounted for 90.5% and 95.3% of those of 125I-HCG 1 day after radiolabeling. These results suggest that 125I-HCG which has been stored for a period since radiolabeling should be purified before use.
Li
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Lei
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Zhang
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D006063:Chorionic Gonadotropin / Q000032:analysis / Q000302:isolation & purification*; D004356:Drug Storage; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007457:Iodine Radioisotopes
D006063:Chorionic Gonadotropin; D007457:Iodine Radioisotopes