In vitro conversion of recombinant human proapolipoprotein A-I to apolipoprotein A-I.
We recently investigated the in vivo conversion of recombinant human proapolipoprotein A-I (rh-Met-proapo A-I) from E. coli to apolipoprotein (apo) A-I in rabbits. In vitro incubation of rh-Met-proapo A-I with rabbit serum produced mature apo A-I3 isoproteins, as determined by the immunoblotting method. However, at the time we were unable to chemically confirm a newly produced protein band which appeared at the position of human apo A-I3. Since then, we have confirmed the amino acid sequence of the protein using a membrane protein sequence technique, and have concluded that it corresponds to human apo A-I3.
Saku
K
K
Department of Internal Medicine, Fukuoka University School of Medicine, Japan.
Bai
H
H
Hirata
K
K
Liu
R
R
Zhang
B
B
Ohkubo
K
K
Yamamoto
K
K
Arakawa
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry*; D001054:Apolipoproteins A / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry*; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry
D000596:Amino Acids; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D011498:Protein Precursors; D011994:Recombinant Proteins