In vitro proton NMR spectroscopy of extracts from human breast tumours and non-involved breast tissue.
Proton (1H) nuclear magnetic resonance (NMR) spectra were obtained from perchloric acid (PCA) extracts of 11 breast tumours and non-involved breast tissue from 7 of the same patients. The spectra were correlated with histopathologic diagnosis. The tumour group consisted of 8 ductal carcinomas, 1 ductal carcinoma with an extensive intraductal component, 1 intraductal carcinoma and 1 fibroadenoma. Higher content of lactate, succinate and phosphocholine and low levels of glucose and inositol were characteristic findings in the tumour group as compared to non-involved breast tissue. 1H NMR spectra of PCA extracted breast specimens provide a comprehensive window into the metabolic activities of the tissue.
Gribbestad
I S
IS
MR-Center, Natural Science Section, SINTEF UNIMED, Trondheim, Norway.
Fjösne
H E
HE
Haugen
O A
OA
Nilsen
G
G
Krane
J
J
Petersen
S B
SB
Kvinnsland
S
S
D002288:Adenocarcinoma, Mucinous / Q000378:metabolism; D000232:Adenofibroma / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D001940:Breast / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism; D005260:Female; D006801:Humans; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008875:Middle Aged; D012265:Ribonucleotides / Q000032:analysis / Q000378:metabolism; D014020:Tissue Extracts / Q000378:metabolism*
D000596:Amino Acids; D012265:Ribonucleotides; D014020:Tissue Extracts; D006859:Hydrogen