Isolation and sequence of a rat glucose-6-phosphate dehydrogenase promoter.
A 935 bp fragment of the rat glucose-6-phosphate dehydrogenase (G6PDH) gene containing promoter activity was isolated using the polymerase chain reaction (PCR). This fragment was sequenced and primer extension analysis showed a transcription initiation site in agreement with the human and mouse genes. Computer analysis of the sequence showed a 60% and 78% similarity to the human and mouse G6PDH sequences, respectively. A TATA box element, TTAAAT, was found and shown to be 100% similar to the human and mouse TATA box elements. Based on sequence comparison, some putative transcriptional regulatory elements were also found.
Rank
K B
KB
Department of Biological Sciences, Western Michigan University, Kalamazoo.
Harris
P K
PK
Ginsberg
L C
LC
Stapleton
S R
SR
D000818:Animals; D001483:Base Sequence; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats
D005954:Glucosephosphate Dehydrogenase