A new imprinted gene cloned by a methylation-sensitive genome scanning method.
We cloned a new imprinted gene by searching for parental-origin-specific CpG methylations using methylation-sensitive two-dimensional genome scanning method. This gene encodes a putative 51 kDa protein with significant similarity to U2 small nuclear ribonucleoprotein auxiliary factor small subunits, an essential mammalian splicing factor, and is located on mouse chromosome 11, of which maternal duplication/paternal deficiency results in a small body.
Hatada
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National Cardiovascular Center Research Institute, Osaka, Japan.
Sugama
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Mukai
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D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D016678:Genome; D008745:Methylation; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics*; D000072668:Splicing Factor U2AF
D004247:DNA; D015226:Dinucleoside Phosphates; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D000072668:Splicing Factor U2AF; C000607495:Zrsr1 protein, mouse; C015772:CpG dinucleotide