Synthesis and ribosome binding properties of model mRNAs modified with undecagold cluster.
The synthesis and purification of short model messenger RNAs modified with undecagold cluster are described. A monoamino undecagold cluster was introduced on the oxidized 3' cis-glycol group of the mRNA followed by reduction of the formed Schiff's base. The stability of the modified mRNA under the conditions used for in vitro messenger RNA translation is studied. The possibility of the formation of a specific translational initiation complex with bacterial ribosomes and modified mRNAs is shown. The results of these experiments indicate that the attachment of an undecagold cluster to a mRNA is a useful tool for electron microscopic and crystallographic studies.
Skripkin
E
E
UPR 9002 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Yusupova
G
G
Yusupov
M
M
Kessler
P
P
Ehresmann
C
C
Ehresmann
B
B
D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism; D006046:Gold / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050607:Organogold Compounds; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D012358:RNA, Transfer, Met / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D050607:Organogold Compounds; D009942:Organometallic Compounds; D012333:RNA, Messenger; D012358:RNA, Transfer, Met; C035797:undecagold; D006046:Gold