Conformation changes of p53 proteins in regulation of murine T lymphocyte proliferation.
This report shows that p53 gene expression is required for proliferation of Concanavalin A (ConA)-stimulated murine T lymphocytes. p53 gene-specific antisense oligodeoxynucleotides strongly inhibited expression of p53 gene in T lymphocytes, causing suppression of ConA-induced cell proliferation. In contrast, the complementary-sense oligomers had no inhibitory effects. Northern and immunoprecipitation-blotting assay data, however, showed no change in p53 mRNA and proteins synthesized in T lymphocytes before and after ConA stimulation. p53 proteins in the resting and ConA-stimulated T cells had different immunoreactivity with specific monoclonal antibodies. p53 from resting T cells only reacted with "wild-type form" p53-specific monoclonal antibody (PAb246); whereas, p53 from ConA-stimulated T cells was recognized by "mutant-form" p53-specific monoclonal antibody (PAb421). These results suggest that the conformation of p53 proteins in resting and ConA-stimulated T cells are different. The p53 conformation changes may be related to the regulation of murine T lymphocyte proliferation.
Wu
J
J
Department of Biochemistry, Nanjing University, P.R. China.
Wang
M
M
Li
X
X
Sheng
Y
Y
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005786:Gene Expression Regulation; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011487:Protein Conformation; D013601:T-Lymphocytes / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000502:physiology*
D016376:Oligonucleotides, Antisense; D016159:Tumor Suppressor Protein p53; D003208:Concanavalin A