Human seminal deoxyribonuclease I (DNase I): purification, enzymological and immunological characterization and origin.
Deoxyribonuclease I (DNase I) was purified from the semen of a 38-year-old male and then characterized. The catalytic properties of the purified enzyme closely resembled those of DNase I purified from the urine of this individual and the following other similarities were observed: molecular masses, iodoacetic acid inactivation kinetics, desialylated isoenzyme patterns. However, the behavior of the purified enzymes determined on several different lectin-affinity chromatography columns differed, which suggests that organ-specific glycosylation of DNase I occurs. Multiple forms of the purified seminal DNase I were demonstrated, each of which had a different pI value separated by isoelectric focusing, which is compatible with the reported existence of genetic polymorphism of seminal DNase I (Sawazaki et al., Forensic Sci Int 1992;57:39-44). Furthermore, enzymological and immunological comparisons of purified seminal and urinary and partially purified prostatic DNases I indicated that the prostate may be one of seminal enzyme source tissues.
Yasuda
T
T
Department of Legal Medicine, Fukui Medical School, Japan.
Sawazaki
K
K
Nadano
D
D
Takeshita
H
H
Nakanaga
M
M
Kishi
K
K
D000328:Adult; D002413:Cations, Divalent; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007461:Iodoacetates / Q000494:pharmacology; D019807:Iodoacetic Acid; D007525:Isoelectric Focusing; D037102:Lectins / Q000378:metabolism; D008297:Male; D010641:Phenotype; D011467:Prostate / Q000201:enzymology; D012661:Semen / Q000201:enzymology*
D002413:Cations, Divalent; D007461:Iodoacetates; D037102:Lectins; D003850:Deoxyribonuclease I; D019807:Iodoacetic Acid