Inhibitory effect of gomisi on reductive metabolism of halothane.
The effect of Gomisi (dried ripe fruit of schizandra chinensis) on chlorodifluoroethylene (CDE) and chlorotrifluoroethane (CTE) formation was investigated. The incubation mixtures for the measurement of reductive metabolites of halothane consisted of liver microsomal suspensions, 3 mM NADPH, extract solution of Gomisi and halothane in 0.1 M potassium phosphate buffer (pH 7.4). The production of CDE and CTE was inhibited by Gomisi in a dose-dependent way. The production were reduced to half in the presence of 0.5% Gomisi extract in the reaction mixture. The results suggest that Gomisi can inhibit the reductive metabolism of halothane in vitro; thus it may protect against halothane-induced hepatitis.
Jiaxiang
N
N
Department of Anesthesiology and Intensive Care Medicine, School of Medicine, Hiroshima University, Japan.
Fujii
K
K
Sato
N
N
Yuge
O
O
D000818:Animals; D017402:Chlorofluorocarbons / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006168:Guinea Pigs; D006221:Halothane / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects
D017402:Chlorofluorocarbons; D004365:Drugs, Chinese Herbal; D003577:Cytochrome P-450 Enzyme System; D006221:Halothane