title: Biomonitoring of polycyclic aromatic hydrocarbons in highly exposed coke plant workers by measurement of urinary phenanthrene and pyrene metabolites (phenols and dihydrodiols).
abstract: A filter combination consisting of an impregnated glass fibre and a control filter was used for the collection of air samples in which gaseous and particulate polycyclic aromatic hydrocarbons (PAHs) were determined. To estimate the loss of lower boiling PAHs, d10-phenanthrene was applied as internal standard. A simple, well-producible method for the determination of 1-, 2-, 3-, 4- and 9-hydroxyphenanthrene, 1,2-, 3,4- and 9,10-dihydroxydihydrophenanthrene, 1-hydroxypyrene and 1,2-dihydroxy-1,2-dihydropyrene is described. By means of personal air samplers the exposure to PAHs of four coke plant employees working at different locations was measured over 4 days. Simultaneously the 24-h urine was collected and stored frozen until analysed. The main excretion product of pyrene is a 1-hydroxypyrene conjugate, whereas phenanthrene is excreted predominantly as dihydrodiol conjugate. As expected, workers on the battery topside were exposed the most and accordingly excreted by far the highest amounts of PAHs. Up to 34.0 micrograms phenanthrol conjugates (total of all isomeric phenols) and 195.5 micrograms dihydrodiol conjugates (total of all isomeric dihydrodiols) were excreted in the 24-h urine (mean of 4 days). The metabolite profiles of five isomeric phenanthrene phenols and three isomeric dihydrodiols exhibited only small percentage variations within one individual whereas significant interindividual differences were observed. These findings may indicate a genetically determined enzyme pattern responsible for the metabolic conversion of PAHs.
authors.affiliation: Biochemisches Institut für Umweltcarcinogene, Grosshansdorf, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Grimmer
authors.forename: G
authors.initials: G
authors.lastname: Dettbarn
authors.forename: J
authors.initials: J
authors.lastname: Jacob
mesh_terms: D000395:Air Pollutants, Occupational / Q000009:adverse effects / Q000493:pharmacokinetics*; D003077:Coke; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D008297:Male; D008452:Maximum Allowable Concentration; D008657:Metabolic Clearance Rate / Q000502:physiology; D010616:Phenanthrenes / Q000009:adverse effects / Q000493:pharmacokinetics*; D010636:Phenols / Q000009:adverse effects / Q000493:pharmacokinetics*; D011083:Polycyclic Compounds / Q000009:adverse effects / Q000493:pharmacokinetics*; D011721:Pyrenes / Q000009:adverse effects / Q000493:pharmacokinetics*
chemical_list: D000395:Air Pollutants, Occupational; D003077:Coke; D010616:Phenanthrenes; D010636:Phenols; D011083:Polycyclic Compounds; D011721:Pyrenes