title: Heterotopic liver transplantation in rats: effect of intrahepatic islet isografts and split portal blood flow on liver integrity after auxiliary liver isotransplantation.
abstract: BACKGROUND
  Auxiliary heterotopic liver grafts atrophy in the absence of portal venous inflow; evidence suggests that an islet-derived hepatotrophic factor may exist in the portal drainage. Here we examine the effects of intrahepatic islet isografts in maintaining hepatocyte integrity in Wistar Furth rats with one of several types of arterialized auxiliary liver isografts.
  
  
  METHODS
  In type 1 procedures the auxiliary liver was interposed into the recipient infrarenal vena cava and perfused through the graft portal vein with caval blood. In type 2 procedures the donor infrahepatic vena cava was anastomosed end-to-side to the recipient vena cava and the recipient portal vein was diverted to the graft portal vein. Both types of auxiliary grafts were arterialized; bile duct drainage was through the duodenum. Syngeneic islets were isolated and embolized into the portal veins of one half of the donor type 1 or native type 2 livers (1500 to 1700 islets). Finally, we performed six type 3 procedures in which a type 2 procedure was performed except that the portal blood flow was split so that the portal vein receiving the splenic, gastric, pancreatic, and duodenal drainage supplied the native liver and that the common mesenteric vein supplied the auxiliary graft with equivalent portal blood flow. Atrophy in heterotopic and native livers were compared for the three models after 3 months.
  
  
  RESULTS
  Intrahepatic islets in type 1 auxiliary liver isografts without portal venous inflow did not prevent graft atrophy. Conversely, native livers deprived of portal venous inflow in our type 2 procedures, regardless of the presence of intrahepatic islet isografts, atrophied relative to auxiliary liver grafts in which portal venous inflow was provided by diverting the recipient's portal vein to the graft. In type 3 recipients atrophy was greater in the native livers than in the grafts.
  
  
  CONCLUSIONS
  The results of our study suggest that islet-derived factors are not sufficient to prevent hepatocellular atrophy in auxiliary rat liver transplantation models and that a potent hepatotrophic factor may exist in the venous drainage of the bowel distal to the duodenum.
authors.affiliation: Department of Surgery, Dalhousie University Faculty of Medicine, Halifax, Nova Scotia, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Yu
authors.forename: X
authors.initials: X
authors.lastname: Wan
authors.forename: J R
authors.initials: JR
authors.lastname: Wright
authors.forename: D
authors.initials: D
authors.lastname: Coddington
authors.forename: H
authors.initials: H
authors.lastname: Bitter-Suermann
mesh_terms: D000818:Animals; D016381:Islets of Langerhans Transplantation; D008099:Liver / Q000098:blood supply* / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D011169:Portal Vein / Q000502:physiology*; D051381:Rats; D011920:Rats, Inbred WF; D012039:Regional Blood Flow; D016042:Transplantation, Heterotopic; D014185:Transplantation, Isogeneic