Rapid enzyme-linked immunosorbent tests for detecting antibodies to Edwardsiella ictaluri in channel catfish, Ictalurus punctatus, using exoantigen.
A rapid enzyme-linked immunosorbent assay (ELISA) was developed to evaluate sera from channel catfish with and without Edwardsiella ictaluri infection. Anti-E. ictaluri antibodies were detected in the sera from 94 of 100 infected catfish. Specific antibodies were not detected in the sera from 100 non-infected fish. The ELISA was based on the Falcon Assay Screening Test (FAST) and was performed in 30 min. An E. ictaluri exoantigen was used in the FAST-ELISA. The immunodominance of the exoantigen was shown by reactivity with monoclonal antibody (MAb) against the predominant epitope of E. ictaluri. The FAST-ELISA was found to be ideal for serodiagnosis and seroepidemiological purposes of detecting and monitoring E. ictaluri antibody responses.
Klesius
P H
PH
Agriculture Research Service, USDA, Auburn, AL 36830.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D001667:Binding, Competitive; D004755:Enterobacteriaceae / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005069:Evaluation Studies as Topic; D005393:Fish Diseases / Q000175:diagnosis* / Q000276:immunology; D007059:Ictaluridae; D013997:Time Factors; D015164:Vaccines, Inactivated / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D015164:Vaccines, Inactivated