title: Urinary metabolites of pinacidil: I. Isolation and identification of the metabolites in rat urine.
abstract: 1. The urinary metabolites of pinacidil administered orally to rats, were analysed by h.p.l.c. Seven metabolites were isolated from rat urine following fractionation on an HP-20 column and purified by t.l.c. 2. The major metabolite was pinacidil-pyridine-N-oxide (M-1). omega-Hydroxypinacidil (M-2) and dealkylpinacidil (M-3) were less abundant metabolites, and minor metabolites were the M-2-aldehyde, in which an intramolecular ring had formed (M-4), M-2-pyridine-N-oxide (M-5), carbamoyl-pinacidil (M-7) and M-7-pyridine-N-oxide (M-6). 3. Metabolic pathways of pinacidil in rats were postulated from the isolated metabolites.
authors.affiliation: Kanzakigawa Laboratory, Shionogi Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006146:Guanidines / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000652:urine*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D020110:Pinacidil; D051381:Rats; D017207:Rats, Sprague-Dawley; D014665:Vasodilator Agents / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000652:urine*
chemical_list: D006146:Guanidines; D014665:Vasodilator Agents; D020110:Pinacidil