A simple treatment of DNA in a ligation mixture prior to electroporation improves transformation frequency.
Kobori
M
M
Department of Molecular Genetics, Osaka University, Japan.
Nojima
H
H
D004269:DNA, Bacterial / Q000235:genetics*; D004278:DNA, Superhelical / Q000235:genetics*; D004558:Electric Stimulation / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D004278:DNA, Superhelical