Expression of a processed and a non-processed form of the integrase protein of HIV-1 in the baculovirus system.
The integrase (IN) protein of HIV-1 was expressed as a processed and a non-processed protein in the eucaryotic baculovirus expression system. In immunoblots we could demonstrate that recombinant baculoviruses containing the complete gag and pol reading frames of HIV-1 expressed a gag/pol precursor polyprotein. The specific proteolytic activity of the recombinant protease on the gag and pol precursor proteins was used for the generation of processed gag (p 17, p 24, p 16) and pol (RT/RNaseH, IN) proteins. The non-processed IN protein, expressed as a polyhedrin fusion protein, was produced at much higher level than the processed protein.
Rodner
B
B
Abteilung Virologie, Universitätskliniken des Saarlandes, Homburg/Saar, Federal Republik of Germany.
Vinga-Martins
C
C
Müller-Lantzsch
N
N
D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D003001:Cloning, Molecular; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli; D015685:Fusion Proteins, gag-pol / Q000235:genetics; D006801:Humans; D007313:Insecta; D019426:Integrases; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D012191:Retroviridae Proteins / Q000235:genetics* / Q000378:metabolism
D015685:Fusion Proteins, gag-pol; D012191:Retroviridae Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases