title: p-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling.
abstract: p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.
authors.affiliation: PB Diagnostic Systems, Inc., Westwood, Massachusetts 02090.
authors.forename: M E
authors.initials: ME
authors.lastname: Annunziato
authors.forename: U S
authors.initials: US
authors.lastname: Patel
authors.forename: M
authors.initials: M
authors.lastname: Ranade
authors.forename: P S
authors.initials: PS
authors.lastname: Palumbo
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry; D000595:Amino Acid Sequence; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D003485:Cyanates / Q000138:chemical synthesis / Q000737:chemistry*; D004076:Digoxigenin / Q000737:chemistry; D004355:Drug Stability; D004958:Estradiol / Q000737:chemistry; D006241:Haptens / Q000737:chemistry; D008301:Maleimides / Q000138:chemical synthesis / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014805:Vitamin B 12 / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D003485:Cyanates; D006241:Haptens; D008301:Maleimides; D010455:Peptides; D013438:Sulfhydryl Compounds; C082056:4-maleimidophenyl isocyanate; D004958:Estradiol; D000469:Alkaline Phosphatase; D004076:Digoxigenin; D014805:Vitamin B 12