Active oxygen-induced modification alters properties of collagen as a substratum for fibroblasts.
Acid-soluble collagen from rat skin was modified by active oxygen in vitro, and properties of the modified collagen as a substratum for fibroblasts were studied. When collagen was treated with ascorbate-copper ion systems, cross-linking and a little degradation occurred rapidly. The cells attached but spread poorly on the modified collagen gel as compared with on the untreated collagen gel. On the other hand, when collagen was treated with H2O2-copper ion systems, only degradation of collagen molecule occurred rapidly. This treatment did not affect the attachment and spreading of the cells on the collagen gel, but when the incubation was continued for a long time, the cells migrated actively and gathered. Thymidine incorporation by the cells was suppressed on both modified collagen gels as compared with that on untreated collagen gel, and the extent of the suppression on the H2O2-copper-treated collagen was larger than that on the ascorbate-copper-treated collagen. These results indicate that the active oxygen-induced cross-linking and degradation significantly alter properties of collagen as a substratum for fibroblasts.
Ohshima
M
M
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Jung
S K
SK
Yasuda
T
T
Sakano
Y
Y
Fujimoto
D
D
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002448:Cell Adhesion; D002455:Cell Division; D048429:Cell Size; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects* / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism* / Q000648:ultrastructure; D005609:Free Radicals; D005782:Gels; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats
D003432:Cross-Linking Reagents; D005609:Free Radicals; D005782:Gels; D003300:Copper; D003094:Collagen; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; D010100:Oxygen