Rapid extraction of methamphetamine and amphetamine in body fluids with Bond Elut SCX cartridges before capillary gas chromatography.
A simple and rapid method for extraction of methamphetamine and amphetamine from human whole blood and urine with Bond Elut SCX cation exchanger cartridges is presented. Detection of the stimulants was made by capillary gas chromatography (GC) with flame ionization detection after derivatization with trifluoroactic anhydride. The compound-containing samples, after mixing with 0.05 M potassium phosphate buffer (pH 7.8), were directly applied to the cartridges, washed with 20 ml of 50% methanol in water and eluted with methanol-HCl (240:1). The recoveries of both methamphetamine and amphetamine were close to 100%. The background noises for Bond Elut SCX cartridge extraction were much smaller than those for Sep-Pak C18 cartridge and diethyl ether extractions.
Kumazawa
T
T
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
Sato
K
K
Seno
H
H
Suzuki
O
O
D000661:Amphetamine / Q000302:isolation & purification*; D001826:Body Fluids / Q000737:chemistry*; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D007475:Ion Exchange Resins; D008694:Methamphetamine / Q000302:isolation & purification*
D007475:Ion Exchange Resins; D008694:Methamphetamine; D000661:Amphetamine