Molecular cloning of a novel human hsp70 from a B cell line and its assignment to chromosome 5.
A 2391-bp cDNA encoding a novel human hsp70, named hsp70 RY, is described. It was cloned from a cDNA library constructed using mRNA derived from an established EBV-transformed B cell line from a patient with leukocyte adhesion molecule deficiency. hsp70 RY is 701 amino acids long, has the characteristic N-terminal ATP-binding domain and the C-terminal peptide binding domain, and contains four potential N-glycosylation sites. Northern blotting revealed a single mRNA species of 3.0 kb in total RNA prepared from the patient's EBV cell line. In situ hybridization localized the single copy hsp70 RY gene to the long arm of chromosome 5 at 5 q31.1-5 q31.2.
Fathallah
D M
DM
Department of Medicine, Massachusetts General Hospital, Charlestown 02129.
Cherif
D
D
Dellagi
K
K
Arnaout
M A
MA
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000737:chemistry*; D001483:Base Sequence; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
D006360:Heat-Shock Proteins