title: Effect of physical training on glucose transporter protein and mRNA levels in rat adipocytes.
abstract: Physical training increases insulin-stimulated glucose transport and the number of glucose transporters in adipocytes measured by cytochalasin B binding. In the present study we used immunoblotting to measure the abundance of two glucose transporters (GLUT-4, GLUT-1) in white adipocytes from trained rats. Furthermore, the abundance of the mRNAs for these proteins and glucose transport was measured. Rats were swim-trained for 10 wk, and adipocytes were isolated from epididymal fat pads. The amount of GLUT-4/adipocyte volume unit was significantly higher in trained animals compared with both age- and cell size-matched animals. The amount of GLUT-4 mRNA was also increased by training and it decreased with increasing age. Furthermore, young age as well as training was accompanied by relatively low GLUT-4 protein/mRNA and relatively high overall GLUT-4 efficiency (recruitability and/or intrinsic activity). GLUT-1 protein and mRNA levels/adipocyte volume did not change with age or training.
authors.affiliation: Department of Medical Physiology, Panum Institute, University of Copenhagen, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Stallknecht
authors.forename: P H
authors.initials: PH
authors.lastname: Andersen
authors.forename: J
authors.initials: J
authors.lastname: Vinten
authors.forename: L L
authors.initials: LL
authors.lastname: Bendtsen
authors.forename: J
authors.initials: J
authors.lastname: Sibbersen
authors.forename: O
authors.initials: O
authors.lastname: Pedersen
authors.forename: H
authors.initials: H
authors.lastname: Galbo
mesh_terms: D019325:3-O-Methylglucose; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D008297:Male; D008757:Methylglucosides / Q000493:pharmacokinetics; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D009124:Muscle Proteins; D009994:Osmolar Concentration; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; C495679:Slc2a1 protein, rat; C495694:Slc2a4 protein, rat; D019325:3-O-Methylglucose