title: Patch graft urethroplasty using dye enhanced laser tissue welding with a human protein solder: a preclinical canine model.
abstract: We investigated the use of the KTP-532 laser to perform a patch graft urethroplasty in 24 adult male dogs using the inner preputial skin as the donor site. In group 1 (12 dogs) repairs were completed with conventional microsuturing techniques, while in group 2 (12 dogs) they were completed using the KTP-532 laser. In the laser welding group the addition of a protein solder (40% human albumin) doped with fluorescein was used. Assessment parameters included a preoperative and postoperative retrograde urethrogram, measurement of intraluminal bursting pressure in the first 6 animals in each group, operative time and histology. Operative time was 42% faster and acute intraluminal bursting pressures were significantly higher in the laser-solder group. No fistulas occurred in the laser-solder group compared to a 50% fistula rate in the suture group. Significant radiographic abnormalities were seen in the urethras of the suture repair group.
authors.affiliation: Department of Urology, New York Hospital-Cornell Medical Center, New York.
authors.forename: D P
authors.initials: DP
authors.lastname: Poppas
authors.forename: D T
authors.initials: DT
authors.lastname: Mininberg
authors.forename: L
authors.initials: L
authors.lastname: Hyacinthe
authors.forename: J R
authors.initials: JR
authors.lastname: Spencer
authors.forename: S M
authors.initials: SM
authors.lastname: Schlossberg
mesh_terms: D000418:Albumins / Q000008:administration & dosage; D000818:Animals; D004285:Dogs; D019793:Fluorescein; D005452:Fluoresceins; D053685:Laser Therapy; D008297:Male; D011183:Postoperative Complications; D016038:Skin Transplantation; D014521:Urethra / Q000473:pathology / Q000601:surgery*
chemical_list: D000418:Albumins; D005452:Fluoresceins; D019793:Fluorescein