title: Crystallization and preliminary crystallographic study of citrate synthase from the thermophilic Archaeon Thermoplasma acidophilum.
abstract: Single crystals of citrate synthase from the Archaeon Thermoplasma acidophilum were obtained in two forms using the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group P222(1), with unit cell dimensions a = 80.9 A, b = 103.8 A, c = 98.3 A and one dimer in the asymmetric unit. Type 2 crystals belong to the monoclinic space group P2(1), with unit cell dimensions a = 53.8 A, b = 173.8 A, c = 86.7 A and beta = 97.1 degrees and two dimers in the asymmetric unit.
authors.affiliation: Department of Biochemistry, University of Bath, Claverton Down, U.K.
authors.forename: R J
authors.initials: RJ
authors.lastname: Russell
authors.forename: D
authors.initials: D
authors.lastname: Byrom
authors.forename: M J
authors.initials: MJ
authors.lastname: Danson
authors.forename: D W
authors.initials: DW
authors.lastname: Hough
authors.forename: G L
authors.initials: GL
authors.lastname: Taylor
mesh_terms: D002950:Citrate (si)-Synthase / Q000302:isolation & purification / Q000648:ultrastructure*; D003461:Crystallography; D011487:Protein Conformation; D013822:Thermoplasma / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D002950:Citrate (si)-Synthase