[Ultrastructural location of enzymes in peripheral blood neutrophils and in cerebrospinal fluid neutrophils in neuroinfections].
Using cytochemical methods the location and activity were determined of alkaline phosphatase, ATP-ase and succinate dehydrogenase as representative enzymes for the metabolic processes in neutrophils isolated from blood and cerebrospinal fluid (CSF) of patients with meningococcal meningoencephalitis as compared with peripheral blood neutrophils in a control group. The study showed presence of phosphatase on the membranes of many intracellular structures. The activity of the enzymes was higher than in the control group in the membranes of neutrophils in blood and CSF. This is explained as an effect of action of the chemotactic factor on the cell membrane and activation of the cell to movements and phagocytosis. ATP-ase activity in peripheral blood neutrophils in controls was found in all membranous structures in the cell. However, in peripheral blood neutrophils and CSF neutrophils in the acute stage of the disease the active enzyme was noted, in the first place, in cell membranes and digesting vacuoles, which reflected probably the direction of metabolic processes for phagocytosis and destroying of bacteria. The activity of succinate dehydrogenase was found in mitochondrial membranes. Peripheral blood and CSF neutrophils showed a high activity of the enzyme. In the CSF cells in acute phase atypical sites of succinate dehydrogenase activity were noted, which was explained as a sign of cell destruction.
Skotarczak
B
B
Katedry i Kliniki Chorób Zakaźnych Pomorskiej AM, Szczecinie.
D000251:Adenosine Triphosphatases / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D005260:Female; D006098:Granulocytes / Q000201:enzymology / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008589:Meningococcal Infections / Q000201:enzymology*; D008590:Meningoencephalitis / Q000201:enzymology*; D008875:Middle Aged; D008928:Mitochondria / Q000737:chemistry / Q000648:ultrastructure; D009345:Neisseria meningitidis / Q000302:isolation & purification; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure*; D013385:Succinate Dehydrogenase / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology / Q000378:metabolism
D013385:Succinate Dehydrogenase; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases
Ultrastrukoturalna lokalizacja enzymów w granulocytach obojetnochłonnych krwi obwodowej i płynu mózgowo-rdzeniowego w prezebiegu neuroinfekcji.