Neutralisation of Cryptosporidium parvum sporozoites by immunoglobulin and non-immunoglobulin components in serum.
Sporozoites of Cryptosporidium parvum were incubated in 1:10 dilutions of immune or non-immune, heat-inactivated lamb serum specimens or serum fractions. The infectivity of treated sporozoites was assessed by inoculating them, per rectum, into five-day-old rats followed by histological examination of their intestines at either three or five days after infection. The infectivity of sporozoites treated with heat-inactivated whole sera was greatly reduced. This neutralisation had both specific and non-specific components. The former was associated with the IgG fraction of hyperimmune serum raised against sporozoites and the latter with a heat-stable, non-dialysable component present in both IgG-depleted hyperimmune serum and uninfected gnotobiotic serum.
Hill
B D
BD
Moredun Research Institute, Edinburgh.
Dawson
A M
AM
Blewett
D A
DA
D000818:Animals; D003457:Cryptosporidiosis / Q000276:immunology / Q000503:physiopathology*; D016785:Cryptosporidium parvum / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D007074:Immunoglobulin G / Q000097:blood; D007136:Immunoglobulins / Q000097:blood*; D009500:Neutralization Tests; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep
D007074:Immunoglobulin G; D007136:Immunoglobulins