title: Increased detection of human immunodeficiency virus antigenemia after dissociation of immune complexes at low pH.
abstract: This study compared the number of patients with detectable human immunodeficiency virus (HIV) antigenemia after immune complex (IC) dissociation by established methods using either 0.5 NCl or 1.5 M glycine buffer. Without IC dissociation, HIV antigen was detected in 43% of patients. After dissociation, the HCl method detected only an additional 7% of patients (P = 0.09), while the glycine method detected an additional 34% (P < 0.001). However, care must be taken in setting the threshold of the standards, and confirmatory neutralization assays should be performed to ensure specificity of HIV antigen enzyme immunoassay after IC dissociation.
authors.affiliation: Department of Internal Medicine, Henry Ford Hospital, Detroit, Michigan 48202.
authors.forename: R A
authors.initials: RA
authors.lastname: Pokriefka
authors.forename: O
authors.initials: O
authors.lastname: Manzor
authors.forename: N P
authors.initials: NP
authors.lastname: Markowitz
authors.forename: L D
authors.initials: LD
authors.lastname: Saravolatz
authors.forename: P
authors.initials: P
authors.lastname: Kvale
authors.forename: R M
authors.initials: RM
authors.lastname: Donovan
mesh_terms: D000936:Antigen-Antibody Complex / Q000097:blood* / Q000302:isolation & purification; D002021:Buffers; D005069:Evaluation Studies as Topic; D005998:Glycine; D016655:HIV Core Protein p24 / Q000097:blood* / Q000302:isolation & purification; D015658:HIV Infections / Q000382:microbiology*; D006801:Humans; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
chemical_list: D000936:Antigen-Antibody Complex; D002021:Buffers; D016655:HIV Core Protein p24; D006851:Hydrochloric Acid; D005998:Glycine