Ornithine decarboxylase activity in Entamoeba invadens.
Growth of E. invadens was paralleled by a concomitant increase in ornithine decarboxylase activity which peaked after 5 days of cultivation in TYI-S-33 medium. Over this period, enzyme activity increased about nine-fold with respect to that present at the start of incubation. Thereafter and coinciding with the onset of the stationary growth phase, enzyme activity started to decline reaching trace levels after 8 days of cultivation. Most of the enzyme remained soluble following centrifugation of amoeba homogenates at 105,000 g. alpha-Difluoromethylornithine failed to affect ornithine decarboxylase activity in vitro and amoeba growth. The enzyme was markedly inhibited by polyamines (putrescine, spermidine and spermine) and 1,4-diamino-2-butanone, a putrescine-analog. The latter arrested proliferation of cells, an effect that could not be reversed by polyamines which by themselves also inhibited growth to a low but significant extent. Our results indicate that polyamine biosynthesis from ornithine is required for growth of E. invadens and that this function is rapidly abolished following entry into the stationary growth phase.
Calvo-Méndez
C
C
Departamento de Genética y Biología Molecular, Instituto Politécnico Nacional, México, D.F., México.
Villagómez-Castro
J C
JC
López-Romero
E
E
D000818:Animals; D000518:Eflornithine / Q000494:pharmacology; D004747:Entamoeba / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000187:drug effects; D011073:Polyamines / Q000494:pharmacology; D004124:p-Dimethylaminoazobenzene / Q000494:pharmacology
D011073:Polyamines; D004124:p-Dimethylaminoazobenzene; D009955:Ornithine Decarboxylase; D000518:Eflornithine