title: Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface.
abstract: OBJECTIVE
  To evaluate the implication of fucose binding sites on the sperm surface in the sperm-zona pellucida (ZP) binding by examining the ability of a fucosylated neoglycoprotein to compete with solubilized zona proteins for sperm binding.
  
  
  METHODS
  The binding of a fucosylated neoglycoprotein to spermatozoa was analyzed in relation to sperm capacitation and the actual acrosomal status. The effect of solubilized ZP material on the binding was examined.
  
  
  METHODS
  Private hospital, public research center, and university department.
  
  
  METHODS
  Healthy volunteers with normal sperm parameters.
  
  
  METHODS
  None.
  
  
  METHODS
  Fluorescence signal detected by fluorescence-activated cell sorting or fluorescence microscopy on spermatozoa incubated with fluorescein isothiocyanate-labeled fucosylated neoglycoprotein.
  
  
  RESULTS
  There was a small subpopulation of spermatozoa that showed a strong binding activity for the fucosylated neoglycoprotein, and the size of this subpopulation increased with the time of exposure to capacitating conditions. The binding sites were located on the head (mainly on the acrosomal region) of acrosome-intact spermatozoa. The neoglycoprotein binding was competitively inhibited by solubilized ZP.
  
  
  CONCLUSIONS
  The data show that a commercially available fucosylated neoglycoprotein competes with solubilized ZP proteins for binding sites on the sperm surface. Further research into the relationship between the neoglycoprotein binding and zona binding is warranted to evaluate the potential diagnostic usefulness of this neoglycoprotein.
authors.affiliation: Center for Reproductive Biology and Medicine, American Hospital of Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Tesarik
authors.forename: C
authors.initials: C
authors.lastname: Mendoza
authors.forename: J P
authors.initials: JP
authors.lastname: Ramirez
authors.forename: J
authors.initials: J
authors.lastname: Moos
mesh_terms: D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012995:Solubility; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000378:metabolism*; D014018:Tissue Distribution; D015044:Zona Pellucida / Q000378:metabolism*
chemical_list: D037102:Lectins; C027112:fucose-binding lectin; D012710:Serum Albumin, Bovine; D005643:Fucose; D016650:Fluorescein-5-isothiocyanate