title: Unique expression pattern of protein kinase C-theta: high mRNA levels in normal mouse testes and in T-lymphocytic cells and neoplasms.
abstract: A 2.2-kb cDNA that contains the entire coding region of mouse protein kinase C-theta (PKC-theta) was cloned from skeletal muscle mRNA using reverse transcription and the polymerase chain reaction (PCR). This clone was used as a probe to study the expression of this PKC isoform in normal and transformed hemopoietic cells and other normal tissues. By far the highest steady-state level of PKC-theta mRNA was found as a 2.8-kb transcript on a Northern blot of poly(A)+ RNA from testes. High levels were also found in skeletal muscle, spleen, T lymphomas and purified normal T lymphocytes, but these tissues and cells expressed two transcripts, 3.3 kb and 3.8 kb. Lower levels of similar size transcripts were found in normal brain, B lymphocytes and B-lymphocytic tumors and cell lines.
authors.affiliation: Molecular Genetics Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: H
authors.initials: H
authors.lastname: Mischak
authors.forename: J
authors.initials: J
authors.lastname: Goodnight
authors.forename: D W
authors.initials: DW
authors.lastname: Henderson
authors.forename: S
authors.initials: S
authors.lastname: Osada
authors.forename: S
authors.initials: S
authors.lastname: Ohno
authors.forename: J F
authors.initials: JF
authors.lastname: Mushinski
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D015342:DNA Probes; D015870:Gene Expression; D008223:Lymphoma / Q000201:enzymology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000201:enzymology*; D013737:Testis / Q000201:enzymology*
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D011493:Protein Kinase C