[In vitro ENU-induced carcinogenesis of rat fetal astroglia--biological character of mutant glial cell].
To investigate glial carcinogenesis in vitro, fetal rat brain cells were cultured and exposed to ENU (approximately 200 micrograms/ml). The cells were passaged weekly thereafter. Morphological changes were observed under the phase contrast microscope. When mutant colonies where the cells lost contact inhibition and grew in a multilayer fashion appeared, the cells were cloned. To assess the biological characters of cells, expression of GFAP, vimentin, A2B5 and p53 product were determined by immunohistochemistry and flow cytometry. Tumor forming ability of the cells was evaluated by both colony forming efficiency in low serum medium (LSM; 2% FBS, 300 cells/100 mm dish) and transplantability to nude mice. Both primary cultured and ENU-treated cells were positive for GFAP and vimentin, but population of A2B5 positive cells was less than 5%, thus indicating that these cells were astroglial in origin. The mutant colonies appeared 7 weeks after ENU treatment. These cells grew rapidly with cell doubling time ranging between 18 to 26 hours, while non-ENU-treated astroglias had a longer cell doubling time (48 to 55 hours). The cloned mutant glial cell lines formed large colonies in LSM (efficiency 20-40%), but astroglial cells did not. The mutant astroglial cells also developed tumors in nude mice. p53 protein was never detected in normal astroglia, however, some glial cells treated by ENU abruptly became p53 positive after several passages. These p53 positive cells formed stratified colonies thereafter. These results indicate that mutant astroglial cells can be induced by a single dose of ENU in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
Hiraga
S
S
Department of Neurosurgery, Osaka University Medical School, Japan.
Arita
N
N
Ohnishi
T
T
Taki
T
T
Yamamoto
H
H
Higuchi
M
M
Hayakawa
T
T
D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D001921:Brain / Q000187:drug effects; D001932:Brain Neoplasms / Q000139:chemically induced; D002478:Cells, Cultured; D005038:Ethylnitrosourea; D005333:Fetus; D005434:Flow Cytometry; D051379:Mice; D008819:Mice, Nude; D009154:Mutation; D009368:Neoplasm Transplantation; D051381:Rats; D017208:Rats, Wistar; D014410:Tumor Stem Cell Assay
D005038:Ethylnitrosourea