Rapid preparation of plasma for 'Stat' coagulation testing.
We undertook to confirm and extend the previous work on the use of microcentrifugation (2 minutes at 11,000 g) to prepare platelet-poor plasma for assay of the prothrombin time, partial thromboplastin time, fibrinogen level, D-dimer, antithrombin III, and dilute Russell viper venom time. We compared results of routinely submitted blood samples by both high-speed and routine (15 minutes at 1800g) centrifugation. We found no significant differences in assay results and concluded that the high-speed technique is a reliable and useful option for minimizing turnaround times for these coagulation assays.
Nelson
S
S
Special Coagulation Laboratory, Denver Veterans Affairs Medical Center, CO.
Pritt
A
A
Marlar
R A
RA
D001769:Blood; D001780:Blood Coagulation Tests / Q000379:methods; D002498:Centrifugation; D006801:Humans; D010976:Platelet Count; D013048:Specimen Handling; D013997:Time Factors