Determination of the relative percentage deuteration of the ribosomal protein S4 by electrospray ionization mass spectrometry.
Electrospray ionization mass spectrometry provides a fast, economical, and accurate new method for determining levels of nonexchangeable deuterium incorporation in proteins derived from organisms grown on deuterated media. Results are shown for determining the percentage deuteration of ribosomal protein S4 from Escherichia coli.
Gulcicek
E E
EE
Analytica of Branford, Inc., Connecticut 06405.
Harrison
D H
DH
Shen
S
S
D000595:Amino Acid Sequence; D003903:Deuterium; D004926:Escherichia coli / Q000378:metabolism; D007553:Isotope Labeling / Q000295:instrumentation / Q000379:methods; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification
D012269:Ribosomal Proteins; C031798:ribosomal protein S4; D003903:Deuterium