title: Reduction in tumour formation on porous polyethylene by collagen immobilization.
abstract: After surface modification with collagen immobilization through covalent bonding, porous polyethylene pieces with an average pore size of 400 microns were implanted subcutaneously into the back of rats for 1 yr. It was found that connective tissues with abundant blood vessels were formed clearly, filling more than 90% of the pore volume and bound firmly to the pore walls. A tumour was found in only one of 24 implanted pieces (4.2%). On the other hand, the virgin porous polyethylene pieces without collagen immobilization exhibited inflammatory reactions within the pores and the connective tissues produced filled only 15% of the pore volume. Formation of a malignant histiocytoma was observed in 11 of the 24 pieces which had been implanted (45.8%). Thus, immobilization of collagen on the surface of an artificial material through covalent bonding proved to be very effective not only for firm bonding with soft connective tissues but also for a reduction of tumour formation.
authors.affiliation: Second Department of Oral and Maxillo-facial Surgery, Kanagawa Dental College, Yokosuka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: T
authors.initials: T
authors.lastname: Kuzuhara
authors.forename: M
authors.initials: M
authors.lastname: Kirigakubo
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Shimura
authors.forename: Y
authors.initials: Y
authors.lastname: Ikada
mesh_terms: D000818:Animals; D003094:Collagen; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced* / Q000517:prevention & control*; D011095:Polyethylenes; D019736:Prostheses and Implants / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011095:Polyethylenes; D003094:Collagen