DNA sequence of the glucosyltransferase gene of serotype d Streptococcus sobrinus.
A glucosyltransferase (GTF) gene was cloned into Escherichia coli from serotype d Streptococcus sobrinus OMZ176. Transformed E. coli strain MI expressed water-insoluble glucan synthesizing activity. Restriction enzyme map of pGT31 extracted from MI shows that the enzyme gene exists in the 6.4-KB PstI-fragment inserted into pBR322 vector. DNA sequence analysis indicates that a single ORF (530-5,300) is located in the PstI-fragment. The putative amino-acid composition (1,590 residues) resembles those of other GTF-I enzymes obtained from serotype g S. sobrinus and serotype h Streptococcus downei. However, at the same positions of the sequence, 18 and 142 amino-acid residues are different between serotype d and g, and serotype d and h GTF-I enzymes, respectively. The differences between serotype d and h GTF-Is are distributed in N and C-terminal regions.
Sato
S
S
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
Inoue
M
M
Hanada
N
N
Aizawa
Y
Y
Isobe
Y
Y
Katayama
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017385:Sequence Homology; D012703:Serotyping; D016986:Streptococcus sobrinus / Q000145:classification / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D005964:Glucosyltransferases