Simultaneous determination of N,N-dimethylformamide, N-monomethylformamide and N-hydroxymethyl-N-methylformamide in rat plasma by capillary gas chromatography.
A capillary gas chromatographic method with nitrogen-phosphorus detection for the simultaneous quantitative determination of N,N-dimethylformamide, and its two metabolites, N-monomethylformamide and N-hydroxymethyl-N-methylformamide, in rat plasma has been developed. The method involves a single extraction step with ethyl acetate-acetone (4:1, v/v). The extract is injected into a fused-silica capillary column coated with Carbowax 20M. A temperature gradient (65-110 degrees C) is applied, and the three products can be separated within 10 min. The quantitation limits, using 25 microliters of rat plasma, for N,N-dimethylformamide, N-monomethylformamide and N-hydroxymethyl-N-methylformamide are 0.4, 0.4 and 2 micrograms/ml, respectively. This method is suitable for toxicokinetic studies in rats.
Rosseel
M T
MT
Heymans Institute of Pharmacology, University of Ghent Medical School, Belgium.
Belpaire
F M
FM
Samijn
N
N
Wijnant
P
P
D000818:Animals; D002849:Chromatography, Gas; D004126:Dimethylformamide / Q000031:analogs & derivatives* / Q000032:analysis* / Q000493:pharmacokinetics; D005559:Formamides / Q000032:analysis*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D012044:Regression Analysis
D005559:Formamides; C042331:N-hydroxymethyl-N-methylformamide; D004126:Dimethylformamide; C002950:methylformamide