[A comparative analysis of the early and late endocytosis of the epidermal growth factor in A431 cells].
Using cultured A431 cells, a comparative analysis was performed of endocytosis stimulated by the epidermal growth factor (EGF) just following the ligand influence (early endocytosis) and after a 3 hour incubation of A431 cells with EGF (delay endocytosis). It is shown that at the early endocytosis the total amount of 125I-EGF, associated with cells, is decreased less than at the delay endocytosis. The decrease in total amount of cell-associated 125I-EGF was accompanied with the increase in the EGF concentration in the incubation medium. The data obtained suggested a lower rate of internalization for delay endocytosis. The Scatchard analysis of 125I-EGF-binding has shown that both high affinity EGF-receptor and low affinity EGF-receptor undergo the down-regulation. The Percoll gradient fractionation of EGF-loaded cells has shown that at the delay endocytosis 125I-EGF is displaced to the fraction of heavy endosomes and lysosomes slower than at the early endocytosis. It is suggested that a delay of EGF-receptor complexes transition from endosomes to lysosomes may arise during delay endocytosis.
Vdovina
I B
IB
Burova
E B
EB
Kornilova
E S
ES
Nikol'skiĭ
N N
NN
D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004705:Endocytosis / Q000187:drug effects* / Q000502:physiology; D004815:Epidermal Growth Factor / Q000493:pharmacokinetics / Q000494:pharmacology*; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D008024:Ligands; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D007457:Iodine Radioisotopes; D008024:Ligands; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
Sravnitel'nyĭ analiz rannego i pozdnego éndotsitoza épidermal'nogo faktora rosta v kletkakh A431.