Mechanisms of platelet aggregation by Streptococcus sanguis, a causative organism in infective endocarditis.
The ability of certain strains of Streptococcus sanguis to aggregate human platelets in vitro may be related to their virulence in the pathogenesis of infective endocarditis. We have studied the mechanisms of aggregation of human platelets by S. sanguis strain NCTC 7863. Platelet aggregation follows incubation of S. sanguis cells with platelet-rich plasma from normal, healthy adults, after a lag of 7-19 min. Platelet aggregation was accompanied by 5-hydroxytryptamine release and thromboxane B2 production. Aggregation was prevented by aspirin and by EDTA. Platelets from two patients with Glanzmann's thrombasthenia did not respond to bacteria. Fixed, washed platelets resuspended in normal plasma were not agglutinated by S. sanguis. Blocking the glycoprotein Ib receptor with a monoclonal antibody inhibited aggregation of PRP. However, S. sanguis did not induce von Willebrand factor (vWF) binding to platelets; nor did the bacteria prevent ristocetin-induced platelet agglutination or vWF binding. The aggregation response was not related to plasma vWF activity levels in normal subjects or in patients with von Willebrand's disease. The platelet response to S. sanguis therefore resembles true aggregation, requiring the cyclo-oxygenase pathway and the presence of glycoprotein IIb/IIIa. The mechanism also involves glycoprotein Ib, but not apparently through irreversible binding of vWF.
Ford
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I
University Department of Haematology, Royal Hallamshire Hospital, Sheffield.
Douglas
C W
CW
Preston
F E
FE
Lawless
A
A
Hampton
K K
KK
D000371:Agglutination; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism; D002478:Cells, Cultured; D004697:Endocarditis, Bacterial / Q000097:blood*; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism; D013290:Streptococcal Infections / Q000097:blood*; D013298:Streptococcus sanguis / Q000502:physiology*; D013915:Thrombasthenia / Q000097:blood; D013929:Thromboxane B2 / Q000096:biosynthesis; D014842:von Willebrand Diseases / Q000097:blood
D012701:Serotonin; D013929:Thromboxane B2; D001241:Aspirin