Rat pancreas kinesin: identification and potential binding to microtubules.
We have demonstrated the presence of kinesin in the secretory pancreatic tissue using SDS-PAGE, immunoblot and immunoelectron microscopy techniques. Polyclonal antibodies were raised against the rat brain kinesin heavy chain and affinity-purified. Immunoblot studies showed that these antibodies were bound to a 116 kDa protein found in rat pancreas crude extracts and in partially purified kinesin fractions. Kinesin identification was also performed by a cosedimentation procedure based on its strong binding to microtubules in the presence of sodium fluoride. The microtubule-kinesin complex was observed by immunoelectron microscopy gold staining. The reversible association of kinesin with microtubules was generated by MgATP.
Malekzadeh-Hemmat
K
K
Unité de Biologie Cellulaire et Physiopathologie Digestives, INSERM U.61, Strasbourg, France.
Gendry
P
P
Launay
J F
JF
D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D001923:Brain Chemistry; D015151:Immunoblotting; D016547:Kinesins / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar
D000255:Adenosine Triphosphate; D016547:Kinesins