Colonization of the respiratory tract with Legionella species.
Pneumonia is the most important manifestation of disease caused by Legionella species. However, it is still not known for certain how these organisms actually enter the lungs. During a 5-month period 318 hospitalized patients without clinical or radiologic features of pneumonia were enrolled in a survey of sputum colonization with Legionella. Diagnostic tests included cultures, direct fluorescent antibody test (DFA) and DNA probe assay of sputum. Colonization was considered to occur when at least one of these tests was positive. According to our definitions, 1.2-10% of of these patients could have been colonized by Legionella spp. However, all cultures were negative and in all but one of these patients the DFA was the only positive test. A case-control study comparing test-positive and test-negative patients failed to identify any different features of disease or epidemiologic characteristics. These findings suggest that a high rate of false-positive DFA results occurred in our study rather than any new evidence of colonization of the respiratory tract with Legionella.
Finkelstein
R
R
Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, Michigan.
Palutke
W A
WA
Wentworth
B B
BB
Geiger
J G
JG
Bostic
G D
GD
D016022:Case-Control Studies; D015342:DNA Probes; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006760:Hospitalization; D006801:Humans; D007875:Legionella / Q000302:isolation & purification*; D008875:Middle Aged; D012137:Respiratory System / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology*
D015342:DNA Probes