Effect of tri-n-butyltin on intracellular Ca2+ concentration of rat cerebellar neurons.
The effect of tri-n-butyltin on the intracellular Ca2+ concentration ([Ca2+]i) of cerebellar neurons dissociated from rats was examined using fluo-3 and a flow cytometer. Tri-n-butyltin at 100 nM or more (up to 1 microM) increased the [Ca2+]i in a dose-dependent manner. The effect of tri-n-butyltin on the [Ca2+]i was greatly reduced under external Ca(2+)-free ([Ca2+]o-free) conditions, suggesting its dependence on the presence of [Ca2+]o. Lower trialkyltins, such as triethyltin and trimethyltin at 1 microM, exerted little or no action on the [Ca2+]i. Therefore, the cytotoxic action of tri-n-butyltin may be different from those of lower trialkyltins.
Oyama
Y
Y
Department of Health Sciences, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Ueha
T
T
Hayashi
A
A
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008345:Manganese / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011921:Rats, Inbred WKY; D014220:Trialkyltin Compounds / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D005456:Fluorescent Dyes; D014220:Trialkyltin Compounds; D008345:Manganese; C011559:tributyltin; D002118:Calcium