title: Adipose tissues from various anatomical sites are characterized by different patterns of gene expression and regulation.
abstract: We have shown previously the presence of brown adipocytes among white fat pads, and proposed the existence of a spectrum of adipose depots according to the abundance of brown fat cells [Cousin, Cinti, Morroni, Raimbault, Ricquier, Pénicaud and Casteilla (1992) J. Cell Sci. 103, 931-942]. In this study, we tried to characterize this spectrum better. We determined in several adipose depots (i) the richness of pre-adipose cells, as assessed by A2COL6 mRNA levels; (ii) whether a fat pad was characterized by a pattern of mRNA expression; (iii) whether this pattern was close related to abundance of brown adipocytes, and (iv) whether the regulation of this pattern by catecholamines under cold exposure or beta-agonist treatment was similar in the different pads. This was achieved by studying proteins involved in glucose and lipid metabolism such as insulin-sensitive glucose transporter (GLUT4), fatty acid synthase, lipoprotein lipase and fatty acid binding protein aP2, as well as beta 3-adrenergic-receptor expression. Among white adipose depots, the periovarian fat pad was characterized by the highest content of pre-adipocytes and of brown adipocytes, and inguinal fat by the highest lipogenic activity potential. There was no close correlation between beta 3-adrenergic-receptor expression and brown adipocyte content in the tissues, as measured by the degree of uncoupling protein (UCP) gene expression. However, in pads expressing UCP mRNA, mRNA levels of beta 3-adrenergic receptor and other markers were increased in parallel. Under cold exposure or beta 3-agonist treatment, a specific up-regulation of GLUT4 expression was observed in interscapular brown adipose tissue. The regional difference described in this study, could participate in preferential fat-pad growth under physiological conditions as well as in pathological situations.
authors.affiliation: Laboratoire de Physiopathologie de la Nutrition, CNRS URA 307, Université Paris VII, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Cousin
authors.forename: L
authors.initials: L
authors.lastname: Casteilla
authors.forename: C
authors.initials: C
authors.lastname: Dani
authors.forename: P
authors.initials: P
authors.lastname: Muzzin
authors.forename: J P
authors.initials: JP
authors.lastname: Revelli
authors.forename: L
authors.initials: L
authors.lastname: Penicaud
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis; D003094:Collagen / Q000096:biosynthesis; D064429:Fatty Acid Synthases / Q000096:biosynthesis; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D007473:Ion Channels; D008565:Membrane Proteins / Q000096:biosynthesis; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1; D003094:Collagen; D064429:Fatty Acid Synthases