title: High throughput DNA sequencing using an automated electrophoresis analysis system and a novel sequence assembly program.
abstract: The combination of high-resolution scanning, image-databasing technology and sequence assembly software makes it possible to assemble contiguous overlapping sequences of DNA in a fraction of the time required by manual methods. This paper describes and evaluates an assembly program that rapidly generates contigs from scanned images and provides a unique ability to verify disagreements between overlapping strands or fragments of complementary DNA sequences on-screen, further increasing sequencing throughput.
authors.affiliation: Millipore Corporation, Burlington, MA 01803.
authors.forename: S
authors.initials: S
authors.lastname: Smith
authors.forename: W
authors.initials: W
authors.lastname: Welch
authors.forename: A
authors.initials: A
authors.lastname: Jakimcius
authors.forename: T
authors.initials: T
authors.lastname: Dahlberg
authors.forename: E
authors.initials: E
authors.lastname: Preston
authors.forename: D
authors.initials: D
authors.lastname: Van Dyke
mesh_terms: D001483:Base Sequence; D004586:Electrophoresis; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software