Probing the functional sites in Naja naja atra (Taiwan cobra) cardiotoxin III with monoclonal antibody.
One monoclonal antibody (mAb) against Naja naja atra cardiotoxin III was prepared by using the hybridoma technique. The cytotoxic activity of cardiotoxin III which inhibited human lymphocyte proliferation was effectively neutralized by the mAb at a molar ratio of antibody to toxin of 1:2. On the contrary, the mAb did not exert a significant inhibition on the hemolytic activity of cardiotoxin III. These results suggest that the functional site responsible for the cytotoxicity might be different from that for the hemolytic activity of cardiotoxin III.
Chang
K L
KL
Department of Biochemistry, Kaohsiung Medical College, Taiwan, ROC.
Chen
Y S
YS
Lin
S R
SR
Chang
L S
LS
Chang
C C
CC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002455:Cell Division; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D004179:Cobra Cardiotoxin Proteins / Q000737:chemistry / Q000276:immunology / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D006461:Hemolysis; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D004179:Cobra Cardiotoxin Proteins; C082202:cardiotoxin III, Naja naja atra