Benzylideneascorbate induces apoptosis in L929 tumor cells.
Sodium 5,6-benzylideneascorbate (SBA), which is known to be an antitumor substance, was found to induce apoptotic cell death of L929 tumor cells directly in a concentration- and time-dependent manner. The dying cells exhibited cell shrinkage, disappearance of cell surface microvilli, chromatin condensation and DNA fragmentation into nucleosomal oligomers characteristic of apoptosis. These results indicate a possible mechanism by which SBA induces tumor regression in vivo.
Tanuma
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Shiokawa
D
D
Tanimoto
Y
Y
Ikekita
M
M
Sakagami
H
H
Takeda
M
M
Fukuda
S
S
Kochi
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D001597:Benzylidene Compounds / Q000633:toxicity*; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005354:Fibrosarcoma / Q000473:pathology / Q000648:ultrastructure; D007700:Kinetics; D051379:Mice; D008854:Microscopy, Electron; D015394:Molecular Structure; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001597:Benzylidene Compounds; D004273:DNA, Neoplasm; D001205:Ascorbic Acid; C070680:zilascorb