title: Genomic structure and promoter activity of the human testis lactate dehydrogenase gene.
abstract: The structure of the gene encoding the human testis-specific isozyme of lactate dehydrogenase (LDH) has been characterized and a regulatory region identified by promoter activity. The single-copy ldh-c gene has two alternative 5' noncoding exons and seven coding exons comprising an approximately 40-kb locus. The gene does not contain the canonical TATA or CAAT promoter sequences, and ribonuclease protection experiments suggest multiple transcription start sites. In the present study an immortalized murine germ cell line was used to detect promoter activity driven by 5' sequence of human ldh-c with lacZ as the reporter gene. Reporter gene activity was nondetectable when promoter constructs were transfected into nongerminal cells.
authors.affiliation: Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University Evanston, Illinois 60208.
authors.forename: L A
authors.initials: LA
authors.lastname: Cooker
authors.forename: C D
authors.initials: CD
authors.lastname: Brooke
authors.forename: M
authors.initials: M
authors.lastname: Kumari
authors.forename: M C
authors.initials: MC
authors.lastname: Hofmann
authors.forename: J L
authors.initials: JL
authors.lastname: Millán
authors.forename: E
authors.initials: E
authors.lastname: Goldberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000737:chemistry*; D005091:Exons; D006801:Humans; D007438:Introns; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D007985:Leydig Cells / Q000201:enzymology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012708:Sertoli Cells / Q000201:enzymology; D013737:Testis / Q000201:enzymology*; D014162:Transfection
chemical_list: D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase