Components required for cytokinesis are important for bud site selection in yeast.
Polarized cell division is a fundamental process that occurs in a variety of organisms; it is responsible for the proper positioning of daughter cells and the correct segregation of cytoplasmic components. The SPA2 gene of yeast encodes a nonessential protein that localizes to sites of cell growth and to the site of cytokinesis. spa2 mutants exhibit slightly altered budding patterns. In this report, a genetic screen was used to isolate a novel ochre allele of CDC10, cdc10-10; strains containing this mutation require the SPA2 gene for growth. CDC10 encodes a conserved potential GTP-binding protein that previously has been shown to localize to the bud neck and to be important for cytokinesis. The genetic interaction of cdc10-10 and spa2 suggests a role for SPA2 in cytokinesis. Most importantly, strains that contain a cdc10-10 mutation and those containing mutations affecting other putative neck filament proteins do not form buds at their normal proximal location. The finding that a component involved in cytokinesis is also important in bud site selection provides strong evidence for the cytokinesis tag model; i.e., critical components at the site of cytokinesis are involved in determining the next site of polarized growth and division.
Flescher
E G
EG
Department of Biology, Yale University, New Haven, Connecticut 06511.
Madden
K
K
Snyder
M
M
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002455:Cell Division; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012331:RNA, Fungal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D013489:Suppression, Genetic; D014157:Transcription Factors
D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D012331:RNA, Fungal; C065889:SPA2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; D012343:RNA, Transfer; C512892:CDC10 protein, S cerevisiae; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins