Binding affinity of the Drosophila melanogaster CF1/USP protein to the chorion s15 promoter.
The Drosophila chorion factor 1/ultraspiracle transcription factor is a developmentally important member of the nuclear hormone receptor superfamily, and this factor most closely resembles the vertebrate retinoid X receptors. We describe here the expression of the CF1/USP protein in an E. coli T7 RNA polymerase-driven expression system. Using partially purified, bacterially produced CF1/USP, we have determined the equilibrium dissociation constant of this protein to its response element on the chorion s15 promoter. The equilibrium binding activity of CF1/USP to its chorion promoter is equivalent to the binding activities of other nuclear hormone receptors to their cognate elements.
Christianson
A M
AM
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
Kafatos
F C
FC
D000818:Animals; D001483:Base Sequence; D002823:Chorion / Q000378:metabolism*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011987:Receptors, Steroid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009838:Oligodeoxyribonucleotides; D011987:Receptors, Steroid; D011994:Recombinant Proteins; D014157:Transcription Factors; C066030:USP protein, Drosophila