title: Selenium content of sheep's milk and its distribution in protein fractions.
abstract: The selenium (Se) distribution in sheep's milk is reported. Skim milk was found to contain the major part (98%) of total milk Se. The Se distribution over casein and whey protein fractions depends on the separation method used, but irrespective of these methods, skim milk Se is mainly associated with the casein fraction (> 68%). Approximately 11%, 4% and 17% Se is removed by dialysis (molecular mass cut-off 10-12 kDa) from skim milk, casein and whey respectively, indicating a major association of Se with milk proteins. This observation is confirmed through Se analysis of individual caseins and whey proteins isolated through ion-exchange chromatography and gel filtration. Se concentrations of the different isolated milk proteins show considerable variations (caseins, 304-750 ng Se/g; whey proteins, 241-806 ng Se/g).
authors.affiliation: Department of Pharmaceutical Sciences, University of Antwerp (UIA), Wilrijk, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Van Dael
authors.forename: L
authors.initials: L
authors.lastname: Shen
authors.forename: R
authors.initials: R
authors.lastname: Van Renterghem
authors.forename: H
authors.initials: H
authors.lastname: Deelstra
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry*; D012643:Selenium / Q000032:analysis*; D012756:Sheep; D014461:Ultracentrifugation; D000067816:Whey Proteins
chemical_list: D002364:Caseins; D008894:Milk Proteins; D000067816:Whey Proteins; D012643:Selenium