Effect of selenium on growth, S-adenosylmethionine and polyamine biosynthesis in human colon cancer cells.
Selenium metabolism and polyamine biosynthesis are linked in their common requirement for S-adenosylmethionine. The effects of selenium supplementation (0.1 to 6.0 ppm) on growth, polyamine biosynthesis and S-adenosylmethionine were examined in two human colon cancer cell lines, WiDr and HT29. WiDr cells were very sensitive to selenium with a significant decrease in 3H-thymidine incorporation and cell number to doses above 0.25 ppm. HT29 cells were less sensitive. In HT29 cells, ornithine decarboxylase activity and its product putrescine decreased in parallel with the growth inhibitory effects of selenium. Similar changes were not noted in WiDr cells. Spermidine and spermine content were conserved in both cell lines exposed to cytotoxic doses of selenium. S-adenosylmethionine was increased in HT29 cells at cytotoxic doses of selenium (1.0 to 6.0 ppm).
McGarrity
T J
TJ
Department of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Peiffer
L P
LP
Hartle
R J
RJ
D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D011700:Putrescine / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000096:biosynthesis*; D012643:Selenium / Q000494:pharmacology*; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D013096:Spermine; D012436:S-Adenosylmethionine; D009955:Ornithine Decarboxylase; D012643:Selenium; D013095:Spermidine; D011700:Putrescine; D013936:Thymidine