Partial cloning of cDNA for hamster liver cholesterol 7 alpha-hydroxylase.
A partial cDNA clone encoding cholesterol 7 alpha-hydroxylase (pHA 7 alpha-3) was isolated from a hamster liver cDNA library using rat P-450 ch7 cDNA (p 7 alpha-11) as a probe. In comparison with the complete base sequence of p 7 alpha-11, pHA 7 alpha-3 lacked about 500 base pairs at the 5' end. Circadian rhythms of mRNA for hamster liver 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol 7 alpha-hydroxylase were investigated with cDNA clones encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase (pRed-227) and pHA 7 alpha-3. Typical circadian rhythms of mRNA for both enzymes and a time lag between the two circadian rhythms were observed. The circadian rhythm of activity for each enzyme corresponded to that of each mRNA.
Hayashi
K
K
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Kurushima
H
H
Saeki
M
M
Amioka
H
H
Kurokawa
J
J
Kuga
Y
Y
Nomura
S
S
Ohkura
Y
Y
Ohtani
H
H
Noshiro
M
M
D000818:Animals; D001483:Base Sequence; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics* / Q000302:isolation & purification; D002940:Circadian Rhythm / Q000235:genetics; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D002790:Cholesterol 7-alpha-Hydroxylase