Hydrolysis of the IciA protein, an inhibitor of DNA replication initiation, by protease Do in Escherichia coli.
The 33 kDa IciA protein, an inhibitor of replication initiation of the Escherichia coli chromosome, was found to be specifically cleaved to 27 kDa fragment by protease Do, the htrA gene product. The 27 kDa polypeptide could no longer interact with the oriC region, and therefore the cleavage-site is likely to reside within the N-terminal DNA-binding domain of the IciA protein. In addition, protease Do was found to localize primarily to the cytoplasm although it also could bind to membranes through an ionic interaction. These results suggest that intracellular breakdown of the IciA protein by protease Do may provide a potential mechanism involving the regulation of initiation of DNA replication in Escherichia coli.
Yoo
S J
SJ
Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea.
Seol
J H
JH
Woo
S K
SK
Suh
S W
SW
Hwang
D S
DS
Ha
D B
DB
Chung
C H
CH
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism* / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D004269:DNA, Bacterial / Q000187:drug effects / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D006868:Hydrolysis; D015151:Immunoblotting; D008970:Molecular Weight; D033903:Periplasmic Proteins; D011817:Rabbits; D012697:Serine Endopeptidases / Q000494:pharmacology*; D013347:Subcellular Fractions
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D033903:Periplasmic Proteins; C068863:argP protein, E coli; C061682:DegP protease; D012697:Serine Endopeptidases