Expression and secretion of outer surface protein (OSP-A) of Borrelia burgdorferi from Escherichia coli.
Outer surface protein A (OspA) is encoded by the ospA gene from Borrelia burgdorferi. This protein induces immunity against infection in mice. The cloning and expression of OspA in Escherichia coli have been previously described, but the secretion of OspA into culture media in E. coli has not yet been reported. In this report we demonstrate that a chimeric OspA protein was secreted into culture media by E. coli when it also harbors the hemolysin secretion genes hlyBD. The OspA fusion protein was also overexpressed from a T7 promoter and purified by immobilized metal ion chromatography. This was possible because the fusion protein contains six histidyl residues in its N-terminus.
Chang
Y F
YF
Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Lauderdale
T L
TL
Lee
W Y
WY
Shin
S J
SJ
Jacobson
R H
RH
Appel
M J
MJ
Lein
D H
DH
D000954:Antigens, Surface / Q000235:genetics* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics; D001428:Bacterial Vaccines; D001483:Base Sequence; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli; D008074:Lipoproteins; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D008074:Lipoproteins; C072045:OspA protein; D011993:Recombinant Fusion Proteins