title: Determination of aspoxicillin in broncho-alveolar lavage fluid by high-performance liquid chromatography with photolysis and electrochemical detection.
abstract: A determination method for the penicillin antibiotic aspoxicillin in broncho-alveolar lavage fluid has been developed, involving high-performance liquid chromatography and post-column photolysis. The method enabled the determination of aspoxicillin at concentrations below 100 ng/ml, with a linear calibration curve at concentrations of 1-1000 ng/ml for 100 microliters of lavage fluid. The detection limit was 1 ng/ml at a signal-to-noise ratio of 3:1.
authors.affiliation: Marugo Laboratory Service Center, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamazaki
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: H
authors.initials: H
authors.lastname: Nakai
authors.forename: M
authors.initials: M
authors.lastname: Miyai
authors.forename: T
authors.initials: T
authors.lastname: Tsubota
authors.forename: K
authors.initials: K
authors.lastname: Asano
mesh_terms: D000658:Amoxicillin / Q000031:analogs & derivatives* / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet
chemical_list: C037485:aspoxicillin; D000658:Amoxicillin