Protein synthesis in different cell lines infected with orthoreovirus serotype 3: inhibition of host-cell protein synthesis correlates with accelerated viral multiplication and cell killing.
In this study, we examined the viral multiplication of human reovirus serotype 3 in mammalian cell lines of different species and tissue origins. All cell lines supported viral multiplication to a similar extent, although the kinetics was significantly different in some of these lines. The appearance of infectious virus was delayed in few cell lines. These cell lines also exhibited a much slower rate of cell killing, and in contrast to the others, did not exhibit an inhibition of the synthesis of host-cell proteins. We conclude that the inhibition correlates with rapid cell killing and viral multiplication, while being unrelated to efficient total viral production.
Danis
C
C
Département de microbiologie et immunologie, Université de Montréal, Québec, Canada.
Lemay
G
G
D000818:Animals; D016923:Cell Death; D002478:Cells, Cultured; D006801:Humans; D012089:Mammalian orthoreovirus 3 / Q000254:growth & development*; D014176:Protein Biosynthesis; D013045:Species Specificity; D014779:Virus Replication