title: Altered isoactin gene expression in the affected bowel segments of the lethal spotted mouse.
abstract: BACKGROUND
  Actin is a key contractile protein associated with the normal differentiation and function of gastrointestinal smooth muscle cells. Distinct changes in gastrointestinal smooth muscle cell morphology and function have been reported for the aganglionic rectum and megacolon of the adult lethal spotted mouse. This study examines what effect these changes in smooth muscle cell morphology and function have on the expression of the actin multigene family in both the aganglionic rectum and megacolon of the lethal spotted mouse.
  
  
  METHODS
  Expression of the smooth muscle and cytoplasmic isoactins was examined by Northern blot analysis of the aganglionic rectum and megacolon of the homozygotic lethal spotted mouse and the equivalent bowel segments of control animals.
  
  
  RESULTS
  The megacolon of the lethal spotted mouse showed a significant increase in gamma-smooth muscle isoactin expression. The aganglionic rectum of the lethal spotted mouse displayed a complex pattern of altered isoactin gene expression that included changes in both gamma-smooth muscle and beta-cytoplasmic isoactin expression. Strain-specific differences in the quantitative levels of isoactin gene expression were observed for the various bowel segments examined in this study.
  
  
  CONCLUSIONS
  These results show that the changes in smooth muscle cell morphology and function observed in the lethal spotted mutant mouse are accompanied by significant alterations in isoactin gene expression.
authors.affiliation: Department of Anatomy, Thomas Jefferson University, Philadelphia, Pennsylvania.
authors.forename: R A
authors.initials: RA
authors.lastname: Liddell
authors.forename: S U
authors.initials: SU
authors.lastname: Mooers
authors.forename: M J
authors.initials: MJ
authors.lastname: Siegman
authors.forename: K M
authors.initials: KM
authors.lastname: McHugh
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D003106:Colon / Q000503:physiopathology; D015870:Gene Expression; D006627:Hirschsprung Disease / Q000235:genetics*; D007422:Intestines / Q000502:physiology*; D008531:Megacolon / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains / Q000235:genetics*; D012007:Rectum / Q000503:physiopathology
chemical_list: D000199:Actins