[Investigation of AZT sensitivity of HIV-1 isolates from patients treated with AZT].
AZT sensitivity was investigated on 6 HIV-1 isolates by culture method. Three of the isolates derived from 2 patients treated with AZT and the others were isolated from patients not treated with AZT. Cultures of 2 isolates (005 and 038) from a patient not treated with AZT did not show cytopathic effect (CPE) in the presence of 0.1 microM or 1.0 microM AZT. Meanwhile, cultures of isolate (043) from a patient treated with AZT for 2 months showed CPE in the presence of 0.1 microM AZT. Furthermore, cultures of isolates from patients treated with AZT for 6 months (043-2) and for 4 months (091) showed CPE in the presence of 1.0 microM AZT. HIV-1 p24 antigen was not detected by ELISA in the supernatants of cultures which did not show CPE. However, HIV-1 p24 antigen was detected in the culture supernatants which showed CPE. These results demonstrated that isolates from patients treated with AZT contained AZT-resistant variants which could replicate in the presence of 1.0 microM AZT.
Saito
T
T
Department of Virology, Kanagawa Prefectural Public Health Laboratories.
Kondo
M
M
Ito
A
A
Nagao
T
T
Imai
M
M
D000293:Adolescent; D000328:Adult; D004352:Drug Resistance, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016655:HIV Core Protein p24 / Q000032:analysis; D015658:HIV Infections / Q000188:drug therapy / Q000382:microbiology; D015497:HIV-1 / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D015215:Zidovudine / Q000494:pharmacology* / Q000627:therapeutic use
D016655:HIV Core Protein p24; D015215:Zidovudine