Alpha 2,6-sialyltransferase predominates in cultured jejunum of suckling rats: it is up-regulated by dexamethasone and secreted during cultivation.
The alpha 2,6-sialyltransferase (alpha 2,6-ST) acting on N-acetyllactosamine is the major sialyltransferase of suckling rat jejunum. Jejunal explants of 7-day-old rats maintained in serum-free or serum-containing organ culture secreted alpha 2,6-ST into the cultivation medium. Dexamethasone (80 nM) stimulates primarily the secreted pool of alpha 2,6-ST. Fetal calf serum promotes the stimulatory effect of dexamethasone also on the bound form of alpha 2,6-ST.
Hamr
A
A
Institute of Physiology, Czech Academy of Sciences, Prague.
Vlasáková
V
V
Kolínská
J
J
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D012799:Sialyltransferases / Q000032:analysis* / Q000378:metabolism; D014357:Trypsin; D015854:Up-Regulation
D003907:Dexamethasone; D012799:Sialyltransferases; D014357:Trypsin