title: Accuracy of pulse oximetry at various haematocrits and during haemolysis in an in vitro model.
abstract: In situations in which it may be impossible and/or unethical to evaluate pulse oximetry in humans, an in vitro model with circulating blood may be a necessity. The main objective was to develop such an in vitro model and, in this model, validate the pulse oximetry technique at various haematocrit levels. The pulsating character of arterial blood flow in a tubing system was simulated by using a specially constructed pressure-regulated roller pump. The tubing system was designed to minimise damage to red blood cells. The pulse oximeter readings (SpO2) were compared with oxygen saturation analyses by a haemoximeter (SaO2). The pulse oximetry readings were recorded at various haematocrit levels and during haemolysis in the SaO2 range 60-100 per cent. At a haematocrit level of 41-44 per cent, there was no correlation between SaO2 and SpO2 readings. After diluting the blood with normal saline to a haematocrit of 10-11 per cent, a good correlation between SaO2 and SpO2 was found. Following haemolysis, the agreement between SaO2 and SpO2 was further improved. Using the developed in vitro model, the results indicate that the accuracy of a pulse oximeter may be dependent on the haematocrit level.
authors.affiliation: Department of Anaesthesiology, University Hospital, Linköping, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Vegfors
authors.forename: L G
authors.initials: LG
authors.lastname: Lindberg
authors.forename: P A
authors.initials: PA
authors.lastname: Oberg
authors.forename: C
authors.initials: C
authors.lastname: Lennmarken
mesh_terms: D006400:Hematocrit; D006461:Hemolysis; D006801:Humans; D008955:Models, Cardiovascular; D010092:Oximetry / Q000379:methods*; D010100:Oxygen / Q000097:blood
chemical_list: D010100:Oxygen