Evidence for a common mechanism of action of interleukin-1 beta, tumor necrosis factor-alpha, and epidermal growth factor on prostaglandin production in human chorion cells.
OBJECTIVE
Although chorion produces prostaglandins in response to interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF alpha), and epidermal growth factor (EGF), little attention has been given to the mechanisms of action of prostaglandin biosynthesis in this tissue.


METHODS
IL-1 beta, TNF alpha, and EGF induced a concentration-related stimulation of prostaglandin E2 (PGE2) biosynthesis in human chorion cells, and this stimulation was enhanced by the addition of exogenous arachidonic acid.


RESULTS
Protein synthesis inhibition with cycloheximide or actinomycin D resulted in complete abrogation of the stimulation of PGE2 production by IL-1 beta, TNF alpha, and EGF. Finally, all three stimulants induced a more rapid recovery of PGE2 production in chorion cells after acetylsalicylic acid pretreatment than controls.


CONCLUSIONS
It is suggested that IL-1 beta, TNF alpha, and EGF all act to stimulate human chorion PGE2 production primarily via induction of prostaglandin endoperoxide synthase activity.
Pollard
J K
JK
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
Thai
D
D
Mitchell
M D
MD
D001241:Aspirin / Q000494:pharmacology; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000502:physiology*; D003609:Dactinomycin / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D016207:Cytokines; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D003609:Dactinomycin; D004815:Epidermal Growth Factor; D003513:Cycloheximide; D015232:Dinoprostone; D001241:Aspirin