[Influence of epinephrine infusion on the metabolism of exogenous lipid emulsions after 70% hepatectomy in the rat].
We investigated the effects of epinephrine on the metabolism of a lipid emulsion administrated to rats after 70% hepatectomy. Sprague-Dawley rats were underwent 70% hepatectomy and then were given lipid total parenteral nutrition (lipid-TPN) for 96 h. To increase catabolism after hepatectomy, epinephrine (Bosmine) infused continuously with the lipid-TPN. With increasing doses of the epinephrine, weight loss and the increased urinary excretion of nitrogen and 3 methyl histidine were observed. This indicated that administration of epinephrine after hepatectomy produced a model of increased surgical stress. As the epinephrine dose increased the linoleic acid content of the hepatic phospholipids increased and the arachidonic acid decreased at 96 h after hepatectomy. Epinephrine apparently inhibited the activity of the delta 5.6 desaturase involved in the biosynthesis of polyunsaturated fatty acids, leading to changes of the cell membrane fatty acid composition that could possibly influence membrane function. Thus, the fatty acid composition of lipid emulsions for TPN should be tailored according to the severity of surgical stress, because the pattern of secretion of stress hormone is dependent on the degree of such stress.
Nakagawa
M
M
First Department of Surgery, Kansai Medical University, Moriguchi, Japan.
Hiramatsu
Y
Y
Kanemaki
T
T
Matsui
Y
Y
Uehara
M
M
Kamiyama
Y
Y
Hioki
K
K
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004837:Epinephrine / Q000493:pharmacokinetics / Q000494:pharmacology*; D005217:Fat Emulsions, Intravenous / Q000493:pharmacokinetics*; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D006498:Hepatectomy; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D050602:Linoleoyl-CoA Desaturase; D008099:Liver / Q000378:metabolism; D008762:Methylhistidines / Q000652:urine; D010289:Parenteral Nutrition, Total; D051381:Rats; D017207:Rats, Sprague-Dawley
D005217:Fat Emulsions, Intravenous; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008762:Methylhistidines; D016718:Arachidonic Acid; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase; C028118:3-methylhistidine; D004837:Epinephrine