Isolation and characterization of two Campylobacter glycine-extracted proteins that bind to HeLa cell membranes.
Two immunogenic proteins of 27 (CBF1) and 29 (CBF2) kDa from enteropathogenic Campylobacter species appear to bind to mammalian cells. We purified these two proteins from a pathogenic and adherent Campylobacter jejuni strain to homogeneity by using acid extraction, preparative gel electrophoresis, and electroelution. Polyclonal rabbit antisera to these proteins were prepared. Immunologic studies indicate that CBF1 corresponds to the PEB1 and CBF2 corresponds to the PEB4 described by Pei et al. (Z. Pei, R. T. Ellison, and M. Blaser, J. Biol. Chem. 226:16363-16369, 1991). Immunogold labeling of a C. jejuni adherent strain with anti-CBF1, anti-CBF2, and anti-PEB1 suggested that CBF1 (PEB1) is surface exposed while CBF2 (PEB4) is not. Analysis of whole-cell extracts from 14 strains by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with 7 M urea and immunoblotting with antisera to CBF1 and CBF2 suggests that CBF proteins from adherent and nonadherent strains are different. Use of purified proteins in a microassay of adherence to cellular membranes indicated that CBF1 was much more adherent than CBF2. Adherence of C. jejuni to viable HeLa cells was markedly reduced with the antiserum to CBF1, whereas the CBF2 antiserum was a poor inhibitor. Purified CBF1 competitively inhibited adherence of whole bacteria to HeLa cells, whereas purified CBF2 was no better a competitor than bovine serum albumin. Adherence of CBF2 was markedly reduced in the presence of Tween 20 or SDS, whereas adherence of CBF1 was reduced only by SDS. We conclude that (i) CBF1 (PEB1) is surface exposed and may be the key protein for C. jejuni adhesion and (ii) CBF2 (PEB4) may be complexed with CBF1 and may passively coadhere with CBF1 under certain experimental conditions. Adherent and nonadherent strains contain different isotypes of these two proteins which could be useful markers of C. jejuni adhesion.
Kervella
M
M
Laboratoire de Bactériologie, Faculté de Médecine Necker-Enfants Malades, Paris, France.
Pagès
J M
JM
Pei
Z
Z
Grollier
G
G
Blaser
M J
MJ
Fauchère
J L
JL
D000918:Antibody Specificity; D001422:Bacterial Adhesion / Q000187:drug effects; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D002167:Campylobacter / Q000737:chemistry*; D004848:Epithelium / Q000378:metabolism; D005998:Glycine; D006367:HeLa Cells; D006801:Humans; D007106:Immune Sera / Q000276:immunology
D001426:Bacterial Proteins; D007106:Immune Sera; D005998:Glycine