Murine mast cells attach to and migrate on laminin-, fibronectin-, and matrigel-coated surfaces in response to Fc epsilon RI-mediated signals.
We have examined the possibility that mouse bone marrow-derived cultured mast cells (BMCMC) have the capacity to attach to and migrate on extracellular matrix components in vitro through the use of time lapse videography. Unactivated mast cells did not display significant interaction with slide flasks coated with either 3% BSA or collagen IV, and Fc epsilon RI-mediated activation of BMCMC did not appreciably increase their attachment and migratory characteristics. Both activated and unactivated BMCMC adhered to surfaces coated with a synthetic IKVAV laminin polypeptide, but this association resulted in the immobilization of the cells to the substrate. BMCMC did not adhere to surfaces coated with laminin, fibronectin or matrigel until Fc epsilon RI-mediated activation, after which they displayed rapid, random movement on these surfaces. Cells continually interacted with laminin, fibronectin or matrigel by flattening, interspaced by periods of movement as rounded cells with small pseudopodia. The mean velocity of BMCMC on laminin, fibronectin or matrigel was similar and averaged approximately 180 microns/hr. The mean velocity of BMCMC on these three substrates was not significantly different from the mean velocity of monocytes on laminin. The movement of BMCMC on these substrates demonstrated a directional tendency. In summary, these results demonstrate that mast cells activated through Fc epsilon RI are capable of attachment to and motion on components of extracellular matrix, and demonstrate one mechanism by which mast cells may migrate to areas of inflammation and wound repair.
Thompson
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HL
Mast Cell Physiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Thomas
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L
Metcalfe
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DD
D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen; D004338:Drug Combinations; D005353:Fibronectins; D007377:Interleukin-3 / Q000494:pharmacology; D007797:Laminin; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology; D011509:Proteoglycans; D017455:Receptors, IgE / Q000502:physiology*; D015398:Signal Transduction
D004338:Drug Combinations; D005353:Fibronectins; D007377:Interleukin-3; D007797:Laminin; D010446:Peptide Fragments; D011509:Proteoglycans; D017455:Receptors, IgE; C059485:matrigel; D003094:Collagen