Inhibition of murine B and T lymphopoiesis in vivo by an anti-interleukin 7 monoclonal antibody.
The effects of interleukin 7 (IL-7) on the growth and differentiation of murine B cell progenitors has been well characterized using in vitro culture methods. We have investigated the role of IL-7 in vivo using a monoclonal antibody that neutralizes IL-7. We find that treatment of mice with this antibody completely inhibits the development of B cell progenitors from the pro-B cell stage forward. We also provide evidence that all peripheral B cells, including those of the B-1 and conventional lineages, are derived from IL-7-dependent precursors. The results are consistent with the rapid turnover of B cell progenitors in the marrow, but a slow turnover of mature B cells in the periphery. In addition to effects on B cell development, anti-IL-7 treatment substantially reduced thymus cellularity, affecting all major thymic subpopulations.
Grabstein
K H
KH
Immunex Research and Development Corporation, Seattle, Washington 98101.
Waldschmidt
T J
TJ
Finkelman
F D
FD
Hess
B W
BW
Alpert
A R
AR
Boiani
N E
NE
Namen
A E
AE
Morrissey
P J
PJ
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001402:B-Lymphocytes / Q000502:physiology*; D005260:Female; D006410:Hematopoiesis; D007075:Immunoglobulin M / Q000032:analysis; D015851:Interleukin-7 / Q000276:immunology / Q000502:physiology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000502:physiology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D015851:Interleukin-7; C039951:anti-IgD; D017493:Leukocyte Common Antigens