Lead transport and binding by human erythrocytes in vitro.
Transport and binding of Pb2+ by human erythrocytes were examined for cell Pb contents in the 1-10 microM range, using the 203Pb isotope. Pb2+ crosses the erythrocyte membrane by the anion exchanger, and can also leave erythrocytes by a vanadate-sensitive pathway, identified with the Ca2+ pump. However, Pb2+ exit is very much less than expected from earlier experiments with resealed erythrocyte ghosts [Simons TJB (1988) J Physiol (Lond) 405:105-113] and the distribution of Pb2+ across the erythrocyte membrane is close to equilibrium. The high ratio of erythrocyte to plasma Pb seen in vivo appears to be due to the presence of a labile Pb(2+)-binding component present in erythrocyte cytoplasm.
Simons
T J
TJ
Biomedical Sciences Division, King's College, Strand, London, UK.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood; D001639:Bicarbonates / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D007854:Lead / Q000097:blood* / Q000378:metabolism; D007856:Lead Radioisotopes; D011753:Pyrones / Q000378:metabolism / Q000494:pharmacology
D001639:Bicarbonates; D007856:Lead Radioisotopes; D011753:Pyrones; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D007854:Lead; D000255:Adenosine Triphosphate; C052408:ethyl maltol; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid