Detection of immunoreactive schizophyllan by solid-phase enzyme-linked immunosorbent assay.
In order to detect immunoreactive schizophyllan (SPG), one of the beta-1,3-glucans in solution, a murine anti-schizophyllan monoclonal antibody, SPG1-HS, was used as a primary antibody for solid-phase enzyme-linked immunosorbent assay (ELISA). Polysaccharide-bovine serum albumin (BSA), and polysaccharide-bovine hemoglobin (Hb) conjugate were prepared as an antigen. The absorbance at 490 nm in ELISA was directly proportional to the SPG concentration in SPG-BSA or SPG-Hb conjugates, allowing the accurate measurement of SPG at concentrations of more than 1.0 microgram/ml. Also, this ELISA detected SPG in SPG-BSA and SPG-Hb conjugates in human serum in a similar manner. These results suggest that this ELISA is applicable for the determination of SPG concentration in SPG-BSA and SPG-Hb conjugates in human tissue and blood.
Hirata
A
A
Research Laboratory, Taito Co., Ltd., Kobe, Japan.
Itoh
W
W
Komoda
M
M
Tabata
K
K
Itoyama
S
S
Sugawara
I
I
D000818:Animals; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D012566:Sizofiran / Q000032:analysis* / Q000097:blood / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D012566:Sizofiran