title: Bacillus cohnii sp. nov., a new, obligately alkaliphilic, oval-spore-forming Bacillus species with ornithine and aspartic acid instead of diaminopimelic acid in the cell wall.
abstract: A group of 20 alkaliphilic Bacillus strains in which all strains revealed the same unique comination of properties--obligate alkaliphily, oval spores distending the sporangium, and ornithine and aspartic acid instead of diaminopimelic acid in the cell wall--was examined. Most of the strains had been isolated by a five-step enrichment and isolation procedure. The G+C content was determined to span a range from 33.5 to 35.0 mol%. Unsaturated fatty acids amounted to 17 to 28% of the total cellular fatty acids. Through DNA-DNA hybridization experiments 11 strains could be grouped in one species. Low homology values with the type strains of validly published Bacillus species with similar G+C contents suggest that these strains belong to a hitherto undescribed species for which the name Bacillus cohnii is proposed. The type strain of the new species is strain RSH (= DSM 6307).
authors.affiliation: DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-3300 Braunschweig, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Spanka
authors.forename: D
authors.initials: D
authors.lastname: Fritze
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis*; D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009952:Ornithine / Q000032:analysis*; D010641:Phenotype; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial; D014812:Vitamin K / Q000031:analogs & derivatives / Q000032:analysis; D024482:Vitamin K 2 / Q000031:analogs & derivatives*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D024482:Vitamin K 2; D014812:Vitamin K; D001224:Aspartic Acid; C062629:menaquinone 7; D009952:Ornithine