Antigenic analysis of Chlamydia pecorum and mammalian Chlamydia psittaci by use of monoclonal antibodies to the major outer membrane protein and a 56- to 64-kd protein.
Monoclonal antibodies were prepared against 40- and 56- to 64-kd antigens of Chlamydia pecorum strain Maeda, which was isolated from a cow with pneumonia. Using the monoclonal antibodies, 5 strains of C pecorum, 25 strains of mammalian and 19 strains of avian C psittaci, 1 strain of C pneumoniae, and 3 strains of C trachomatis were analyzed for immunologic reactivity by use of the indirect immunofluorescent test. Monoclonal antibody analysis revealed immunologic relatedness between C pecorum and mammalian strains of C psittaci, which were completely differentiated from the other avian strains. Bovine strains were distinguished from ovine strains. Antigenic diversity was observed for bovine and ovine strains. Feline- and guinea pig-derived strains were shown to be immunologically different from bovine and ovine strains. Results provide the basis for typing and epidemiologic study of bovine and ovine strains of C pecorum and C psittaci.
Kuroda-Kitagawa
Y
Y
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
Suzuki-Muramatsu
C
C
Yamaguchi
T
T
Fukushi
H
H
Hirai
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D003411:Arvicolinae; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001717:Birds; D002415:Cats; D002417:Cattle; D002689:Chlamydia / Q000276:immunology* / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000382:microbiology / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000276:immunology* / Q000302:isolation & purification; D006168:Guinea Pigs; D006801:Humans; D008970:Molecular Weight; D012756:Sheep; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins