High-performance liquid chromatographic method for the determination of indapamide in human whole blood.
A sensitive, accurate, and reproducible high-performance liquid chromatographic procedure for the analysis of indapamide in human whole blood is reported. After a single-step liquid-liquid extraction at pH 6.6 using diethyl ether, indapamide was eluted from a Nucleosil C18 5-microns column with 80 mM ammonium acetate, pH 3.5-acetonitrile-2-propanol (65:30:5, v/v/v). The peak height versus whole blood concentration was linear over the range 10.0-500 ng/ml using ultraviolet detection. The mean absolute recovery of indapamide using the described assay was 87.4%. The inter- and intra-day accuracy and precision were within 9.6% of the actual values for all concentrations investigated. Furthermore, this procedure was applied to the analysis of whole blood samples from healthy subjects receiving a single 2.5-mg oral dose of indapamide.
Miller
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Analytical Research Center, L.A.B. Bio-Research International Inc., Senneville, Montreal, Quebec, Canada.
Dadgar
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Lalande
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D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004986:Ether; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007190:Indapamide / Q000097:blood*; D007700:Kinetics; D011786:Quality Control
D004986:Ether; D007190:Indapamide