title: Molecular cloning and sequencing of a carp cDNA encoding mitogen-activated protein kinase kinase.
abstract: We have cloned a full-length cDNA encoding the mitogen-activated protein kinase kinase (MKK) from a carp liver cDNA library. The cDNA contains 1970 bp with a single open reading frame encoding a 397 amino acid protein. By comparing with known MKK sequences from other species, carp MKK is 78%, 80%, 76% and 58% identical to rat MKK1, rat MKK2, Xenopus MKK and Drosophila MKK.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: C J
authors.initials: CJ
authors.lastname: Huang
authors.forename: M S
authors.initials: MS
authors.lastname: Lee
authors.forename: G D
authors.initials: GD
authors.lastname: Chang
authors.forename: F L
authors.initials: FL
authors.lastname: Huang
authors.forename: T B
authors.initials: TB
authors.lastname: Lo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D048369:MAP Kinase Kinase 1; D048370:MAP Kinase Kinase 2; D020929:Mitogen-Activated Protein Kinase Kinases; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D008934:Mitogens; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D048369:MAP Kinase Kinase 1; D048370:MAP Kinase Kinase 2; D020929:Mitogen-Activated Protein Kinase Kinases