Effects of carbon tetrachloride, ethanol and acetaldehyde on diethylnitrosamine-induced hepatocarcinogenesis in rats.
The modifying action of experimentally induced chronic liver injury on diethylnitrosamine (DEN) hepatocarcinogenesis was investigated using a minimal treatment protocol. A single dose of DEN (15 mg/kg b.w.) was administered as a carcinogen to 1-day-old Sprague-Dawley rats. From 3 weeks of age rats received repeated intraperitoneal injections of carbon tetrachloride (CCl4), or 10% ethanol or 5% acetaldehyde in the drinking water for 9 weeks. Combinations of CCl4 and ethanol or acetaldehyde were also tested. Morphology, immunohistochemistry for glutathione S-transferase-placental form, and incidence and quantity of preneoplastic lesions of the livers were studied. The chronic CCl4 administration produced complete or incomplete liver cirrhosis and exerted a strong promoting effect on the development of neoplastic nodules. Ethanol alone revealed no cirrhogenous or tumor-promoting effect, but enhanced both actions of CCl4. Acetaldehyde increased only the cirrhogenous effect of CCl4.
Cho
K J
KJ
Department of Anatomic Pathology, Korea Cancer Center Hospital, Nowon-Ku, Seoul.
Jang
J J
JJ
D000079:Acetaldehyde / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D004052:Diethylnitrosamine; D000431:Ethanol / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000032:analysis*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley
D004052:Diethylnitrosamine; D000431:Ethanol; D002251:Carbon Tetrachloride; D005982:Glutathione Transferase; D000079:Acetaldehyde