Differentiation between mouse-virulent and -avirulent strains of Toxoplasma gondii by a monoclonal antibody recognizing a 27-kilodalton antigen.
Using a murine monoclonal antibody, we were able to differentiate between mouse-virulent and -avirulent strains of Toxoplasma gondii. Monoclonal antibody TB6G5 was reactive with eight clinical mouse-avirulent isolates but not with mouse-virulent laboratory strains RH and BK. The antibody-reactive antigen was identified by indirect immunofluorescence and immunoblot as a 27-kDa cytoplasmic protein expressed by tachyzoites as well as by bradyzoites.
Bohne
W
W
Institute of Hygiene and Microbiology, University of Würzburg, Germany.
Gross
U
U
Heesemann
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000737:chemistry; D003429:Cross Reactions; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D013045:Species Specificity; D014122:Toxoplasma / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity*; D014123:Toxoplasmosis / Q000276:immunology; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000382:microbiology; D014774:Virulence / Q000276:immunology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan