Rapid methods for identification of Staphylococcus aureus when both human and animal staphylococci are tested: comparison with a new immunoenzymatic assay.
A new immunoenzymatic assay (IEA) for the identification of Staphylococcus aureus strains of both human and animal origin was compared with rapid commercial kits. The sensitivities and specificities of the commercial kits varied from 90.2 to 96% and 90.8 to 93.7%, respectively. The IEA did not give any false-negative or false-positive results, while commercial kits gave high percentages of false-positive results among clumping factor-positive non-S. aureus strains. The IEA is particularly useful for isolates for which identification is doubtful, for large-scale epidemiological studies, and for identifying isolates from animals as S. aureus.
Guardati
M C
MC
Institute of Microbiology, University of Genova, Italy.
Guzmàn
C A
CA
Piatti
G
G
Pruzzo
C
C
D000818:Animals; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000662:veterinary; D013210:Staphylococcus / Q000145:classification / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification*