title: Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method.
abstract: The presence of Legionella spp. in cooling tower water was investigated by using the polymerase chain reaction. Total Legionella spp. detection was performed with 20-mer 5S rRNA complementary DNA sequence primers, and specific Legionella pneumophila detection was performed with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legionella spp., and 14 of these contained L. pneumophila.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Koide
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: N
authors.initials: N
authors.lastname: Kusano
authors.forename: F
authors.initials: F
authors.lastname: Higa
mesh_terms: D001431:Bacteriological Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D006761:Hospitals; D007875:Legionella / Q000302:isolation & purification*; D016952:Legionella pneumophila / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial