Comparison of techniques for detecting antigens of Giardia lamblia and Cryptosporidium parvum in faeces.
OBJECTIVE
To compare the use of commercial monoclonal antibody test systems--the Giardia CEL IF test and the Crypto CEL IF test--for the detection of Giardia lamblia and Cryptosporidium parvum antigens in faeces with conventional techniques.


METHODS
Sensitivity and specificity were evaluated using preparations of cysts of G lamblia and purified oocysts of C parvum. Evaluation of 59 random faecal samples passing through the Department of Clinical Parasitology, Hospital for Tropical Diseases, London, was carried out for both organisms.


RESULTS
The fluorescence staining techniques proved more sensitive than other tests routinely used for diagnosis.
Tee
G H
GH
Department of Clinical Parasitology, Hospital for Tropical Diseases, London.
Moody
A H
AH
Cooke
A H
AH
Chiodini
P L
PL
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis*; D003457:Cryptosporidiosis / Q000175:diagnosis*; D016785:Cryptosporidium parvum / Q000276:immunology*; D005243:Feces / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D016829:Giardia lamblia / Q000276:immunology*; D005873:Giardiasis / Q000175:diagnosis*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D011933:Reagent Kits, Diagnostic