Carboxyl-terminal heparin-binding fragments of platelet factor 4 retain the blocking effect on the receptor binding of basic fibroblast growth factor.
Platelet factor 4 (PF-4) blocks the binding of basic fibroblast growth factor (bFGF) to its receptor. In the present study, we constructed carboxyl-terminal fragments, which represent the heparin-binding region of the PF-4 molecule, and examined whether these synthetic peptides retain the blocking effects on the receptor binding of bFGF. Synthetic peptides inhibited the receptor binding of bFGF. Furthermore, they inhibited the migration and tube formation of bovine capillary endothelial cells in culture (these phenomena are dependent on endogenous bFGF).
Sato
Y
Y
Department of Internal Medicine 1, Oita Medical University.
Waki
M
M
Ohno
M
M
Kuwano
M
M
Sakata
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D010978:Platelet Factor 4 / Q000494:pharmacology*; D017468:Receptors, Fibroblast Growth Factor / Q000187:drug effects* / Q000378:metabolism
D010446:Peptide Fragments; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D010978:Platelet Factor 4; D006493:Heparin