The influence of C18 fatty acids on the growth of fibroblasts of different degrees of transformation in culture.
Normal human skin fibroblasts (HSF) and cells from 2 benign (3T3 and 3T6B) and 1 malignant (3T6D) fibroblast lines were grown in the presence of C18 fatty acids in the concentration range 0-100mg/1 culture medium. The culture medium consisted of 10% FBS and 90% DMEM, the cells were dosed with various concentrations of fatty acid in ethanol as carrier 24 hours post-trypsinisation, and the viability determined using Trypan Blue exclusion after a further 48 hours. The threshold between cytostatic and cytotoxic effects was considered to be that concentration of fatty acid which limited cell number to that of the controls at the end of the post-trypsinisation recovery period. All the fatty acids dosed exhibited growth limitation potential, to a greater or lesser extent, which varied depending on the degree of unsaturation of the molecule and transformation of the fibroblasts. While lipoperoxide levels increased with degree of unsaturation of the fatty acids, the stereoisomeric configuration of the unsaturations was not a significant factor. Eicosanoid production requires cis-configuration only, thus eicosanoids are unlikely to be involved. The lipoperoxide levels are more likely to reflect non-enzymic peroxidation of fatty acids released from membrane phosphoglycerides.
Davidson
B C
BC
Department of Medical Biochemistry, University of the Witwatersrand Medical School, Parktown, South Africa.
Giangregorio
A
A
Girao
L A
LA
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002461:Cell Line, Transformed; D005227:Fatty Acids / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism; D051379:Mice
D005227:Fatty Acids; D008054:Lipid Peroxides