Identification, characterization and sequence of Candida albicans repetitive DNAs Rel-1 and Rel-2.
Two moderately repetitive DNA elements, Rel-1 and Rel-2, were identified in a screen for clones that hybridized to a Candida albicans minichromosome. Rel-1, a 223-bp sequence, is C. albicans-specific. The 2789-bp Rel-2 sequence hybridizes weakly to C. stellatoidia DNA but not to DNA from several other yeast species. Genomic Southern-blot analysis indicated that Rel-1 and Rel-2 are often closely associated in the genome, suggesting that they may be subsequences of a larger repetitive element. Small subrepeats are located in the nucleotide sequence of both clones. Hybridization demonstrated that Rel-2 contains both repetitive and unique DNA sequences. The repetitive DNA is present on most, and perhaps all, C. albicans chromosomes. The unique sequence maps to chromosome 7; however, in some strains, it is also present on additional chromosomes.
Thrash-Bingham
C
C
Department of Microbial Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543-4000.
Gorman
J A
JA
D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal; D016521:Electrophoresis, Gel, Pulsed-Field; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004271:DNA, Fungal