Introduction of a series of alkyl thiomaltosides, useful new non-ionic detergents, to membrane biochemistry.
We synthesized a series of non-ionic detergents, alkyl thiomaltosides, and investigated their properties and usefulness. We solubilized membrane proteins of Vibrio parahaemolyticus using the detergents. With octyl thiomaltoside, nonyl thiomaltoside, decyl thiomaltoside, or undecyl thiomaltoside, we observed satisfactory solubilization of the membrane proteins. Alkyl thiomaltosides possessing longer alkyl chains showed better solubilization than ones possessing shorter chains. H(+)-translocating ATPase (F0F1), which is localized in the cytoplasmic membrane (inner membrane), was solubilized with the detergents, and the solubilized enzyme showed much higher specific activity than that solubilized with octyl glucoside or heptyl thioglucoside, other useful non-ionic detergents. 5'-Nucleotidase, which seems to be an outer membrane protein, was also efficiently solubilized with the alkyl thiomaltosides. Membrane proteins of Escherichia coli were also efficiently solubilized with the detergents. Octyl thiomaltoside and nonyl thiomaltoside were removed fairly rapidly on dialysis. Decyl thiomaltoside was removed slowly, and undecyl thiomaltoside and dodecyl thiomaltoside were difficult to remove by dialysis.
Izawa
S
S
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University.
Sakai-Tomita
Y
Y
Kinomura
K
K
Kitazawa
S
S
Tsuda
M
M
Tsuchiya
T
T
D015720:5'-Nucleotidase / Q000737:chemistry*; D003902:Detergents / Q000138:chemical synthesis / Q000737:chemistry*; D003956:Dialysis; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D008320:Maltose / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility; D014736:Vibrio parahaemolyticus / Q000737:chemistry
D003902:Detergents; D008565:Membrane Proteins; D004024:Dicyclohexylcarbodiimide; D008320:Maltose; D015720:5'-Nucleotidase; D006180:Proton-Translocating ATPases