title: Ovarian and hemolymph ecdysteroid titers during vitellogenesis in Macrobrachium rosenbergii.
abstract: Changes in ovarian and hemolymph ecdysteroid concentration and composition during vitellogenesis have been investigated in the freshwater prawn Macrobrachium rosenbergii. Free ecdysteroids (20-hydroxyecdysone and ecdysone) in hemolymph increased in concentration during vitellogenesis from zero at stage 0 to 1.5 ng/ml at stage I to 7.3 ng/ml in mature, stage IV animals. 20-Hydroxyecdysonoic acid (1.2 ng/ml) was detected in the stage IV hemolymph. Ovarian-free ecdysteroid concentration, expressed as nanograms per gram of tissue, fell during vitellogenesis from 83.2 ng/g at stage 0, non-pigmented tissue to 14.2 ng/g at stage IV tissue, being minimal at stage I (6.3 ng/g). However, expression of ovarian free ecdysteroid content as nanograms per ovary revealed a rise from 7.7 ng/ovary at stage 0, nonpigmented tissue to 28.3 ng/ovary at stage IV, again being minimal at stage I (2.0 ng/ovary). 20-Hydroxyecdysonoic acid and ecdysonoic acid were identified at ovarian stages II-III and stage IV.
authors.affiliation: Department of Biochemistry, University of Liverpool, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Young
authors.forename: S G
authors.initials: SG
authors.lastname: Webster
authors.forename: H H
authors.initials: HH
authors.lastname: Rees
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D026461:Ecdysteroids; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006458:Hemolymph / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007447:Invertebrate Hormones / Q000302:isolation & purification / Q000378:metabolism*; D008745:Methylation; D010053:Ovary / Q000737:chemistry / Q000378:metabolism*; D033541:Palaemonidae / Q000378:metabolism*; D011863:Radioimmunoassay; D013256:Steroids / Q000302:isolation & purification / Q000378:metabolism*; D014818:Vitellogenesis / Q000502:physiology*
chemical_list: D026461:Ecdysteroids; D007447:Invertebrate Hormones; D013256:Steroids