GNFFRFamide: a novel FMRFamide-immunoreactive peptide isolated from the sheep tapeworm, Moniezia expansa.
The widespread distribution of FMRFamide-immunoreactivity in platyhelminth nervous systems has been demonstrated immunocytochemically. Here we report the isolation and primary structure of the first platyhelminth FMRFamide-related peptide (FaRP) from the tapeworm, Moniezia expansa. The peptide was found to have a molecular mass of 785 Da and a primary structure of Gly-Asn-Phe-Phe-Arg- Phe-NH2 (GNFFRFamide). Acid alcohol extracts of this platyhelminth contained only this single FaRP which had a phenylalanyl (F) residue in the variable position 3 from the C-terminal occupied by either methionyl (M), leucyl (L) or isoleucyl (I) residues in most other FaRPs.
Maule
A
A
Comparative Neuroendocrinology Research Group, Queen's University of Belfast, Northern Ireland.
Shaw
C
C
Halton
D
D
Thim
L
L
D000595:Amino Acid Sequence; D000818:Animals; D002589:Cestoda / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D019835:FMRFamide; D015801:Helminth Proteins / Q000737:chemistry* / Q000302:isolation & purification; D007447:Invertebrate Hormones / Q000737:chemistry*; D008969:Molecular Sequence Data; D008989:Monieziasis / Q000469:parasitology*; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000469:parasitology*; D013056:Spectrophotometry, Ultraviolet
D015801:Helminth Proteins; D007447:Invertebrate Hormones; D009479:Neuropeptides; C082029:GNFFRF-amide protein, Moniezia expansa; D019835:FMRFamide