title: The synthetic retinoid (4-hydroxyphenyl)retinamide decreases collagen expression in vitro and in the tight-skin mouse.
abstract: OBJECTIVE
  We documented the effect of the retinoid (4-hydroxyphenyl)retinamide on collagen expression in a tissue culture and in an animal model of scleroderma.
  
  
  METHODS
  We used RNA analysis, chloramphenicol acetyltransferase assays, organ culture, and histologic evaluation.
  
  
  RESULTS
  We showed that (4-hydroxyphenyl)retinamide decreases alpha 1(I) collagen messenger RNA and transcription in cultured cells, and decreases collagen levels in the dermis of tight-skin mice.
  
  
  CONCLUSIONS
  These results provide a basis for further experiments to address the efficacy of (4-hydroxyphenyl)retinamide in the treatment of scleroderma.
authors.affiliation: Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire 03755-3844.
authors.forename: A M
authors.initials: AM
authors.lastname: Delany
authors.forename: C E
authors.initials: CE
authors.lastname: Brinckerhoff
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D004195:Disease Models, Animal; D017313:Fenretinide / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D012333:RNA, Messenger / Q000032:analysis; D012595:Scleroderma, Systemic / Q000235:genetics; D012867:Skin / Q000737:chemistry / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D017313:Fenretinide; D003094:Collagen