title: African swine fever virus encodes a serine protein kinase which is packaged into virions.
abstract: Nucleotide sequencing of the SalI j region of the virulent Malawi (LIL20/1) strain of African swine fever virus (ASFV) identified an open reading frame (ORF), designated j9L, with extensive similarity to the family of protein kinases. This ORF encodes a 35.1-kDa protein of 299 amino acids which shares 24.6% amino acid identity with the human pim-1 proto-oncogene and 21.0% identity with the vaccinia virus B1R-encoded protein kinase. The ASFV ORF contains the motifs characteristic of serine-threonine protein kinases, with the exception of the presumed ATP-binding site, which is poorly conserved. The ORF was expressed to high levels in Escherichia coli, and the recombinant enzyme phosphorylated a calf thymus histone protein on serine residues in vitro. An antibody raised to an amino-terminal peptide of the ASFV protein kinase was reactive with the recombinant protein in Western immunoblot analyses and was used to demonstrate the presence of the protein kinase in ASF virions.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
authors.forename: S A
authors.initials: SA
authors.lastname: Baylis
authors.forename: A H
authors.initials: AH
authors.lastname: Banham
authors.forename: S
authors.initials: S
authors.lastname: Vydelingum
authors.forename: L K
authors.initials: LK
authors.lastname: Dixon
authors.forename: G L
authors.initials: GL
authors.lastname: Smith
mesh_terms: D000358:African Swine Fever Virus / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005814:Genes, Viral; D006801:Humans; D008295:Malawi; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D011494:Protein Kinases / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051573:Proto-Oncogene Proteins c-pim-1; D011519:Proto-Oncogenes; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics; D014771:Virion / Q000201:enzymology* / Q000235:genetics
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; C496634:PIM1 protein, human; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1