Phospholipid hydroperoxide glutathione peroxidase: full-length pig blastocyst cDNA sequence and regulation by selenium status.
We have isolated and sequenced an apparent full-length cDNA clone for phospholipid hydroperoxide glutathione peroxidase (Genbank accession number L12743) from a pig blastocyst cDNA library. The sequence encodes a polypeptide of 170 amino acids, including a TGA-encoded selenocysteine at residue 46, with a calculated M(r) of 19,492 Da. Use of this clone in Northern blot analysis of Se-deficient rat liver revealed that phospholipid hydroperoxide glutathione peroxidase mRNA levels were little affected by Se deficiency, whereas classical glutathione peroxidase mRNA levels were decreased by 90% in the same samples. Lastly, liver phospholipid hydroperoxide glutathione peroxidase mRNA levels were not elevated in female rats, in contrast to classical glutathione peroxidase.
Sunde
R A
RA
Dept. of Food Science & Human Nutrition, College of Human Environmental Sciences, University of Missouri, Columbia 65211.
Dyer
J A
JA
Moran
T V
TV
Evenson
J K
JK
Sugimoto
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000201:enzymology*; D015152:Blotting, Northern; D004247:DNA; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D005979:Glutathione Peroxidase / Q000096:biosynthesis* / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D015345:Oligonucleotide Probes; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D012643:Selenium / Q000172:deficiency / Q000378:metabolism* / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D012643:Selenium