title: Identification of Moraxella-like bacteria isolated from caprine and ovine nasal flora.
abstract: Twenty four Moraxella related bacterias were isolated from healthy caprine and ovine nasal swabs and were investigated by classic biochemical tests and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins in comparison with 9 reference strains. Proteolytic and haemolytic strains were investigated by electron microscopy. The biochemical results clustered field isolates in four groups corresponding to Branhamella and Moraxella species. Proteolytic, haemolytic and fimbriated field isolates showed the same morphological structure and biochemical features as Moraxella bovis. SDS-PAGE results indicated that DICE coefficient between a field isolate and the corresponding reference strain can be as 62.5%; 41.7% and 36% respectively for the groups 1, 3 and 4. The group 2 showed a similarity percentage over 75% with the reference strain Moraxella nonliquefaciens. This results indicated that a non proteolytic but haemolytic bacteria, closely related to Moraxella nonliquefaciens was commonly isolated from small ruminants nasal flora. These animals can also be hosts of a subspecies of Moraxella bovis.
authors.affiliation: Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
authors.forename: A
authors.initials: A
authors.lastname: Kodjo
authors.forename: A
authors.initials: A
authors.lastname: Moussa
authors.forename: E
authors.initials: E
authors.lastname: Borges
authors.forename: Y
authors.initials: Y
authors.lastname: Richard
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D009016:Moraxella / Q000737:chemistry / Q000302:isolation & purification*; D009297:Nasal Mucosa / Q000382:microbiology*; D016870:Neisseriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins