title: Whole-mount in situ hybridization in the mouse embryo: gene expression in three dimensions.
abstract: Non-isotopic whole-mount in situ hybridization of mRNA is a novel technique that has greatly facilitated the precise three-dimensional localization of transcripts from genes whose expression is important during development. This methodology has recently been applied to the study of the mouse embryo and offers particular advantages over conventional procedures.
authors.affiliation: AFRC Centre for Genome Research, University of Edinburgh, UK.
authors.forename: B
authors.initials: B
authors.lastname: Rosen
authors.forename: R S
authors.initials: RS
authors.lastname: Beddington
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis*; D016610:Tissue Embedding; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger