title: Interaction of activin A and gonadal steroids on FSH secretion from primary cultured rat anterior pituitary cells.
abstract: To clarify the mechanism of FSH secretion from the pituitary induced by activin A, we studied the interaction between activin A and gonadal steroids in inducing FSH release from primary cultured female rat pituitary cells in serum-free medium. The basal release of FSH was stimulated by activin A, testosterone (T) and progesterone (P), and T and P also facilitated basal FSH release stimulated by activin A. The GnRH-stimulated FSH release was facilitated by activin A, P and 17 beta-estradiol (E2), but suppressed by T. The effect of activin A on GnRH-stimulated FSH release was facilitated by P, but not affected by T or E2. These findings suggested that the interaction between activin A and T or P may be involved in the regulation of FSH secretion during the estrus cycle in rats.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, University of Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyake
authors.forename: M
authors.initials: M
authors.lastname: Irahara
authors.forename: K
authors.initials: K
authors.lastname: Shitukawa
authors.forename: T
authors.initials: T
authors.lastname: Yasui
authors.forename: T
authors.initials: T
authors.lastname: Aono
mesh_terms: D028341:Activins; D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D007265:Inhibins / Q000494:pharmacology*; D007700:Kinetics; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D006133:Growth Substances; D011994:Recombinant Proteins; D028341:Activins; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007265:Inhibins; D005640:Follicle Stimulating Hormone