title: Satisfactory separation and MS-MS spectrometry of six surfactins isolated from Bacillus subtilis natto.
abstract: We optimized HPLC conditions for the first time to achieve satisfactory separation of six surfactins, two of which were novel, isolated from Bacillus subtilis natto. Peptide sequences were analyzed by sophisticated MS-MS analysis, performed with a pair of two surfactins with different fatty acid substitutions. Fragment peaks were divided into two series; one was a series of the ions which carried fatty acid, and thus were characterized by the fatty acid mass difference, and the other was a series of the ions which consisted of pure peptide and thus showed a superimposable pattern between a pair of surfactins.
authors.affiliation: Tokyo College of Pharmacy, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Oka
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: M
authors.initials: M
authors.lastname: Homma
authors.forename: H
authors.initials: H
authors.lastname: Ishii
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Mogami
authors.forename: A
authors.initials: A
authors.lastname: Motizuki
authors.forename: H
authors.initials: H
authors.lastname: Morita
authors.forename: K
authors.initials: K
authors.lastname: Takeya
authors.forename: H
authors.initials: H
authors.lastname: Itokawa
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D055666:Lipopeptides; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic
chemical_list: D001426:Bacterial Proteins; D055666:Lipopeptides; D010456:Peptides, Cyclic; C009365:surfactin peptide