Osteoblasts release a soluble factor that modulates immunoglobulin secretion by mature B cells.
An osteogenic member, MBA-15 cell line, from marrow stroma compartment, was examined for its ability to regulate mature lymphoma B cell line functions. The effect of MBA-15 cell secreting factor/s on cell proliferation, immunoglobulin secretion and isotype switch of 29M4.1 IgM+ mature B cell line was examined. The factor causes temporal arrest in cell proliferation, augments immunoglobulin secretion and has no effect on isotype switching. The results revealed a direct effect of the MBA-15 factors on the B cell functions. Factors secreted by other osteoblastic cells (ROS 17/2.8 and MC-3T3-E1) possess the same biological activities. In contrast, factors secreted by an endothelial adipocyte stromal cell line 14F1.1 lack these properties.
Benayahu
D
D
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Altboum
I
I
Zipori
D
D
Wientroub
S
S
Zan-Bar
I
I
D000704:Analysis of Variance; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D002453:Cell Cycle; D002460:Cell Line; D002999:Clone Cells; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016393:Lymphoma, B-Cell; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology*; D014407:Tumor Cells, Cultured
D007070:Immunoglobulin A; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D008070:Lipopolysaccharides