Syncytiotrophoblastic localization of the human growth hormone variant mRNA in the placenta.
The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental growth hormone, which replaces pituitary GH in maternal blood throughout pregnancy. By means of mRNA competitive hybridization using 32P-labelled and unlabelled 30 bases long oligonucleotides, we first optimized specific hybridization conditions. In situ hybridization was then performed to locate the GH-V mRNA encoding placental growth hormone. The hGH-V gene appears expressed in the placental syncytiotrophoblast. Unlike the CS-A and CS-B genes (both encoding hPL) which are expressed uniformly in the syncytiotrophoblast, the GH-V mRNA is located in a few syncytiotrophoblast cells only.
Scippo
M L
ML
Laboratoire d'Endocrinologie, Institut de Pathologie, Liège (Sart Tilman), Belgium.
Frankenne
F
F
Hooghe-Peters
E L
EL
Igout
A
A
Velkeniers
B
B
Hennen
G
G
D001483:Base Sequence; D005260:Female; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D010926:Placental Hormones / Q000096:biosynthesis / Q000235:genetics*; D011247:Pregnancy; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014327:Trophoblasts / Q000737:chemistry* / Q000378:metabolism
C064980:GH2 protein, human; D010926:Placental Hormones; D012333:RNA, Messenger; D013006:Growth Hormone