Teaching electron diffraction and imaging of macromolecules.
Electron microscopic analysis can be used to determine the three-dimensional structures of macromolecules at resolutions ranging between 3 and 30 A. It differs from nuclear magnetic resonance spectroscopy or x-ray crystallography in that it allows an object's Coulomb potential functions to be determined directly from images and can be used to study relatively complex macromolecular assemblies in a crystalline or noncrystalline state. Electron imaging already has provided valuable structural information about various biological systems, including membrane proteins, protein-nucleic acid complexes, contractile and motile protein assemblies, viruses, and transport complexes for ions or macromolecules. This article, organized as a series of lectures, presents the biophysical principles of three-dimensional analysis of objects possessing different symmetries.
Chiu
W
W
Werna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Schmid
M F
MF
Prasad
B V
BV
D001703:Biophysics / Q000193:education*; D003461:Crystallography; D004500:Education, Graduate; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008958:Models, Molecular
D046911:Macromolecular Substances