Measurement of labeling index of DNA polymerase alpha in human brain tumors. Comparative study with labeling indices of BUdR in vitro and Ki-67.
Proliferative activity of 28 human brain tumors was estimated by simultaneous measurement of DNA polymerase alpha, Ki-67 and bromodeoxyuridine (BUdR) labeling indices on microscopic tissue preparations stained immunologically with monoclonal antibodies using a peroxidase technique. All the antigens were exclusively found in the nucleus. The labeling index of BUdR was lower than those of the other indicators. The values of the DNA polymerase alpha labeling index were almost the same as those of the Ki-67 labeling index. Simultaneous measurement of these parameters may provide more useful information on tumor cell growth kinetics than that of a single parameter.
Nishizaki
T
T
Department of Neurosurgery, Yamaguchi University, School of Medicine, Japan.
Orita
T
T
Kamiryo
T
T
Akimura
T
T
Kajiwara
K
K
Ikeda
N
N
Ohshita
N
N
Ito
H
H
Sasaki
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000473:pathology*; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000502:physiology*; D002648:Child; D004257:DNA Polymerase II / Q000032:analysis*; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis
D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D004257:DNA Polymerase II; D001973:Bromodeoxyuridine