Residual Ph- cells in chronic myeloid leukemia: detection and usefulness.
Bone marrow cells from patients with chronic myeloid leukemia (CML) in in vitro long-term bone marrow culture (LTBMC) show an impaired survival of Philadelphia (Ph) positive cells and, in a proportion of patients, the emergence of Philadelphia negative hemopoiesis. The standard conditions of in vitro cultures provide optimal purging effect. Selected patients can now have autologous bone marrow transplants (ABMT) with in vitro purged cells.
Chang
J
J
Department of Experimental Hematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Coutinho
L H
LH
Santos
A M
AM
Brereton
M
M
Harrison
C J
CJ
Testa
N G
NG
D016026:Bone Marrow Transplantation; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D010677:Philadelphia Chromosome; D013997:Time Factors; D014407:Tumor Cells, Cultured