Endotoxin clearance through whole body rinse out in relation to histologic integrity in the rat.
The effects of normothermic whole body rinse out (NWBRO) with FC-43 perfluorocarbon emulsion on the course of endotoxic shock were examined in male Wistar rats. These animals, conscious and unrestrained, were infused over 10 min with 60 mg/kg of E. coli endotoxin (LD100) and observed for 6 hr. The rats developed hypotension, plasma extravasation, metabolic acidosis, increase in serum GPT and GOT, and extensive hemorrhages in the small intestine. Another group of rats was subjected to blood substitution with FC-43 emulsion (artificial blood) 30 min after endotoxin infusion, until the hematocrit dropped to less than 3%, after which 6-9 ml of donor blood was exchange transfused with the emulsion. NWBRO caused a transitory rise in the GOT level, but more importantly it resulted in 98% endotoxin clearance, improved blood alkaline reserves, trimmed elevated GPT and GOT levels, and attenuated hemorrhage and epithelial necrosis in the small intestine.
Voynikov
T
T
Department of Pharmacology, Kinki University School of Medicine, Osaka, Japan.
Nikolova
I
I
Yamanishi
Y
Y
Higashino
H
H
Maeda
M
M
Suzuki
A
A
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001802:Blood Substitutes / Q000627:therapeutic use*; D004731:Endotoxins / Q000097:blood*; D005466:Fluorocarbons / Q000627:therapeutic use*; D007421:Intestine, Small / Q000473:pathology; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012772:Shock, Septic / Q000097:blood / Q000473:pathology / Q000628:therapy*; D013154:Spleen / Q000473:pathology
D001802:Blood Substitutes; D004731:Endotoxins; D005466:Fluorocarbons; C008807:perfluorotributylamine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase