Fluorescence properties of plastoquinol, ubiquinol and alpha-tocopherol quinol in solution and liposome membranes.
It was found that plastoquinol-9, ubiquinol-10 and alpha-tocopherol quinol show intrinsic fluorescence in organic solvents and in liposomes. Their fluorescence spectra in solution showed the presence of one emission band with maximum intensity in the range 319.0-327.0 nm for plastoquinol and 321.5-326.5 nm for alpha-tocopherol quinol, which is the longest wavelength shifted in polar solvents. The emission band at about 371 nm for ubiquinol was not sensitive to solvent polarity. For all three prenylquinones the fluorescence quantum efficiency changed significantly in solvents of different polarities, being the highest in ethanol and the lowest in hexane in the case of plastoquinol and alpha-tocopherol quinol, whereas ubiquinol fluorescence showed the opposite effect. These spectral parameters were applied to determination of prenylquinol localization in liposome membranes.
Kruk
J
J
Department of Physiology and Biochemistry of Plants, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Strzałka
K
K
Leblanc
R M
RM
D008081:Liposomes; D010971:Plastoquinone / Q000031:analogs & derivatives* / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000737:chemistry; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D008081:Liposomes; D012996:Solutions; D014451:Ubiquinone; D014810:Vitamin E; C024838:alpha-tocopherol quinol; C003165:plastoquinol; D024502:alpha-Tocopherol; C003741:ubiquinol; D010971:Plastoquinone