Insulin-like growth factor-I is a serum component stimulating growth of human neuroblastoma.
Human non-autocrine neuroblastoma cells SK-N-SH and LF require serum for proliferation in vitro. We wished to determine the role of serum-borne insulin-like growth factor I (IGF-I) as mitogen for these cells. Introduction of the monoclonal antibody alpha-IR3 against human IGF-I receptor reduced proliferation in the presence of fetal bovine serum (FBS). IGF-I (5 nM) was as effective as FBS (10%) in stimulating proliferation. Porcine insulin mimicked the effects of IGF-I, but at a 1000-fold higher concentration. The antibody alpha-IR3 reduced growth stimulated by IGF-I more effectively than growth stimulated by insulin. Thus, proliferation of human non-autocrine neuroblastoma cells can be effectively manipulated by exogenous IGF-I.
Burke
T W
TW
Division of Developmental Oncology Research, Mayo Clinic, Rochester, Minnesota.
Vuk-Pavlović
S
S
D002455:Cell Division / Q000187:drug effects*; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007700:Kinetics; D009447:Neuroblastoma; D013997:Time Factors; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016895:Culture Media, Serum-Free; D007334:Insulin-Like Growth Factor I