[Determination of amantadine by pre-labeling with 3-(7'-methoxycoumarin-3'-carbonyl)-benzoxazoline-2-thione and high-performance liquid chromatography with fluorescence detection].
3-(7'-Methoxycoumarin-3'-carbonyl)-benzoxazoline-2-thione (MCBT) was synthesized as a precolumn fluorescent labeling reagent for amantadine for use in high-performance liquid chromatography (HPLC). Amantadine was derivatized quantitatively into a fluorescent compound through the amino group by treatment with MCBT at room temperature for 5 min in aqueous 95% acetonitrile solution in the presence of 4-dimethylaminopyridine. The derivative was subjected to HPLC on TSK gel ODS-80TM (250 x 4.6 mm i.d.) with methanol-water (10:1) as the mobile phase and monitored with an excitation wavelength of 355 nm and an emission wavelength of 405 nm. The limit of detection was 2.0 micrograms/ml in the urine. This method was satisfactory with respect to simplicity and precision to quantify amantadine spiked in the urine.
Fujino
H
H
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Ueno
I
I
Goya
S
S
D000547:Amantadine / Q000652:urine*; D001583:Benzoxazoles / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000138:chemical synthesis*; D005456:Fluorescent Dyes; D006801:Humans
C082311:3-(7'-methoxycoumarin-3'-carbonyl)benzoxazoline-2-thione; D001583:Benzoxazoles; D003374:Coumarins; D005456:Fluorescent Dyes; D000547:Amantadine