Potential application of LASER/microbe bioassay technology for determining water-soluble vitamins in foods.
A microbiological technique was developed for quantitating niacin by determining microbial growth rates in response to the amount of vitamin available. Unlike the current official AOAC method, the new procedure for niacin measured the growth rates during the early exponential growth phase rather than during the stationary phase. Lactobacillus plantarum was used to determine niacin to a lower limit of 100 pg/mL. The assay time was approximately 6 h, compared with 16-24 h for the current AOAC method. The extent of microbial growth was determined by differential light scattering of a LASER beam. Multiple photodetectors were integrated with a computer system to collect and analyze the data. The use of differential light scattering to determine 8 water-soluble vitamins under stationary phase conditions demonstrated the potential application of the new technology for microorganisms and foods.
Anderson
E M
EM
U.S. Food and Drug Administration, Division of Nutrition, Washington, DC 20204.
Angyal
G N
GN
Weaver
C M
CM
Felkner
I C
IC
Wolf
W R
WR
Worthy
B E
BE
D001681:Biological Assay; D003470:Culture Media; D005504:Food Analysis; D007202:Indicators and Reagents; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D007834:Lasers; D008027:Light; D009525:Niacin / Q000032:analysis / Q000494:pharmacology; D012542:Scattering, Radiation; D014815:Vitamins / Q000032:analysis* / Q000494:pharmacology
D003470:Culture Media; D007202:Indicators and Reagents; D014815:Vitamins; D009525:Niacin