title: [Intra-macrophage lipid particles collected by bronchoalveolar lavage: incidence and diagnostic value].
abstract: This study aimed to determine the incidence and diagnostic value of fat-laden alveolar macrophages obtained by bronchoalveolar lavage (BAL). In 128 patients, including 66 patients admitted for multiple trauma, 158 BAL were carried out. However, 41 BAL from 32 patients were excluded because of poor quality of samples (not enough macrophages, too many ciliated cells, or haemorrhage). All the patients were intubated and mechanically ventilated, having pulmonary infiltrates on the chest film. BAL samples were examined after staining with oil-red-O. They were considered to be positive when more than 5% of alveolar macrophages contained fat droplets. Among them 14 out of 47 patients (30%) without multiple trauma were positive; 7/14 had never been given any intravenous lipid infusion, and 5/14 had aspiration pneumonia (as opposed to 3/32 patients with negative BAL). Further 27 patients out of the 49 (55%) with multiple trauma were positive. Among them 10/49 had clinical evidence of fat embolism, however, only 7/10 had positive samples. All these last ten patients had been given intravenous lipid infusions. The rate of positive alveolar macrophages was correlated neither with the plasma triglyceride concentration, nor the Fracture Index Score, nor the delay between the end of the lipid infusion and the BAL. There was no significant difference in PaO2/FIO2 ratio between the patients with positive and negative BAL. Positive BAL was significantly associated with lipid infusions. The data therefore suggest that the presence of fat-laden alveolar macrophages are associated with various pathological pulmonary conditions, particularly aspiration pneumonia and lipid infusions.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Service d'Anesthésie-Réanimation, Hôpital Edouard-Herriot, Lyon.
authors.forename: P
authors.initials: P
authors.lastname: Gratadour
authors.forename: J M
authors.initials: JM
authors.lastname: Védrinne
authors.forename: C
authors.initials: C
authors.lastname: Guillaume
authors.forename: M C
authors.initials: MC
authors.lastname: Gagnieu
authors.forename: J
authors.initials: J
authors.lastname: Motin
mesh_terms: D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D003422:Critical Care; D004620:Embolism, Fat / Q000175:diagnosis* / Q000503:physiopathology; D005217:Fat Emulsions, Intravenous; D005260:Female; D006801:Humans; D008055:Lipids / Q000032:analysis*; D016676:Macrophages, Alveolar / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D011015:Pneumonia, Aspiration / Q000175:diagnosis* / Q000503:physiopathology
chemical_list: D005217:Fat Emulsions, Intravenous; D008055:Lipids
vernacular_title: Particules lipidiques intramacrophagiques recueillies par lavage bronchoalvéolaire: fréquence et intérêt diagnostique.