Immunophenotypic and molecular analysis of a case of lymphocytic interstitial pneumonia.
A case of lymphocytic interstitial pneumonia was studied immunophenotypically and with molecular methods in order to clarify its lymphocytic clonality. The patient, a 43 year old Japanese female, underwent lobectomy for a suspected malignant lymphoma as no clear diagnosis could be made from the biopsy specimen. An ill-demarcated, yellowish and elastic firm lesion measuring 60 x 35 x 20 mm in size was located in the peripheral part of the middle lobe of the right lung. Histologically, the alveolar, peribronchial-vascular and subpleural interstitia within the lesion were thickened markedly with severe cellular infiltration largely composed of small lymphocytes with germinal centers. Immunostaining revealed immunoglobulin (Ig) kappa and Ig lambda-bearing cells to be evenly distributed, suggestive of polyspecificity. Immunoglobulin gene analysis further demonstrated the unrearranged germ-line DNA but no rearranged band. These results strongly indicated a reactive process rather than a neoplastic nature for the lesion.
Kawabuchi
B
B
Department of Pathology, University of Tokyo, Japan.
Tsuchiya
S
S
Nakagawa
K
K
Sasajima
Y
Y
Mori
S
S
Machinami
R
R
Tsuchiya
E
E
D000328:Adult; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis / Q000235:genetics; D016130:Immunophenotyping; D008175:Lung Neoplasms / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology*; D011658:Pulmonary Fibrosis / Q000235:genetics / Q000276:immunology / Q000473:pathology*
D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains