title: Middle latency responses to electrical stimulation of the auditory nerve in unanaesthetized guinea pigs.
abstract: Middle latency responses (MLR) to sinusoidal and pulsatile electrical stimulation (ES) of the cochlea and to acoustical stimulation (AS) were evaluated in awake guinea pigs with chronically implanted electrodes. The ear, which was later electrically stimulated, was deafened by local intracochlear application of gentamicin, the opposite ear was left intact. Waveforms and P1-P2 interpeak intervals of the electrically evoked MLR (ES-MLR) were similar to those evoked by acoustical stimulation of the intact ear (AS-MLR) and the latencies of the ES-MLR were shorter by about 1-3 ms. Thresholds of ES-MLR in the frequency range 0.5-32 kHz increased with increasing ES frequency (slope 3.2 dB/octave), thresholds were 3.5-9.5 dB lower for intracochlear than for extracochlear ES. Dynamic ranges for ES-MLR varied between 6-20 dB. MLR amplitude-intensity functions for ES were steeper (slope 2-12 microV/dB) than those for AS (slope 0.2-2 microV/dB). Maximal ES-MLR amplitudes exceeded usually 1.5-3 times the amplitudes of the acoustically evoked MLR. Both types of stimulations evoked larger MLR amplitudes to contralateral stimulation than to ipsilateral stimulation (average ratio = 4.1 +/- 2.2 for AS and 3.3 +/- 2.2 for ES). Because of the relatively long latency and therefore insensitivity to electrical artifact, the ES-MLR can be used for the evaluation of different strategies of the electrical stimulation of the cochlea in awake guinea pig.
authors.affiliation: Institute of Experimental Medicine, Czechoslovak Academy of Sciences, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Popelár
authors.forename: J
authors.initials: J
authors.lastname: Syka
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D003638:Deafness / Q000503:physiopathology; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D005072:Evoked Potentials, Auditory / Q000502:physiology*; D006168:Guinea Pigs; D011930:Reaction Time / Q000502:physiology; D000159:Vestibulocochlear Nerve / Q000502:physiology*