Novel magnesium ion-selective microelectrodes based on a neutral carrier.
Two types of Mg(2+)-selective microelectrodes based on a synthetic neutral carrier and containing poly-(vinyl chloride) (PVC) are described. The first is applicable to intracellular Mg2+ assays and suffers no interference from alkali and all other alkaline-earth metal cations. The second can be used for determining intra- as well as extracellular Mg2+ activities, but Ca2+ and especially lipophilic cations (e.g. acetylcholine) may interfere. Cyclohexanone is used for dissolving the PVC-containing membrane phases thus avoiding problems encountered with the much more volatile tetrahydrofuran. The procedure gives a higher output of working microelectrodes and can also be applied with other membrane phases.
Schaller
U
U
Department of Organic Chemistry, Swiss Federal Institute of Technology (ETH), Zürich.
Spichiger
U E
UE
Simon
W
W
D002118:Calcium / Q000032:analysis; D003512:Cyclohexanones; D008274:Magnesium / Q000032:analysis*; D008567:Membranes, Artificial; D008670:Metals; D008839:Microelectrodes; D011143:Polyvinyl Chloride; D011786:Quality Control
D003512:Cyclohexanones; D008567:Membranes, Artificial; D008670:Metals; C036468:cyclohexanone; D011143:Polyvinyl Chloride; D008274:Magnesium; D002118:Calcium