title: A comparison of hypotensive and non-hypotensive hemorrhage on Fos expression in spinally projecting neurons of the paraventricular nucleus and rostral ventrolateral medulla.
abstract: The protein, Fos, detected immunohistochemically, was used to identify neurons in the brain that were activated after hemorrhage in the conscious rat. Spinally projecting neurons in the paraventricular nucleus (PVN) and rostral ventrolateral medulla (RVLM) were identified by the presence of rhodamine-labeled latex beads which had been previously injected into the upper thoracic spinal cord. On the experimental day, conscious rats underwent either (1) withdrawal of 4 ml of blood from a carotid cannula (n = 8) which reduced mean arterial pressure from 96.6 +/- 2.7 to 42.7 +/- 7.1 mmHg, (2) withdrawal of 2 ml of blood (n = 4) which did not affect mean arterial pressure. Animals that were not hemorrhaged were used as controls (n = 6). After the 4 ml hemorrhage, dense concentrations of Fos-positive cell nuclei were found in the lamina terminalis, supraoptic nuclei (SON), PVN and in the medulla. In contrast, the density of Fos-positive cells in 2 ml-hemorrhaged rats was not different from controls except in the SON and in the medial PVN in 2 of 4 rats. After the 4 ml hemorrhage 14.4 +/- 1.2% of the spinally projecting neurons in the PVN and 22.7 +/- 6.1% in the RVLM expressed Fos (P < 0.001 compared to control). After the 2 ml hemorrhage the proportion was 12.2 +/- 3.1% in the PVN (P < 0.001 compared control) but only 5.4 +/- 2.2% in the RVLM (P > 0.05 compared to control). The results suggest that spinally projecting neurons in the PVN and RVLM participate in the reflex responses to hemorrhage.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Vic., Australia.
authors.forename: E
authors.initials: E
authors.lastname: Badoer
authors.forename: M J
authors.initials: MJ
authors.lastname: McKinley
authors.forename: B J
authors.initials: BJ
authors.lastname: Oldfield
authors.forename: R M
authors.initials: RM
authors.lastname: McAllen
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000503:physiopathology*; D005453:Fluorescence; D015870:Gene Expression; D016762:Genes, fos; D007022:Hypotension / Q000503:physiopathology*; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013495:Supraoptic Nucleus / Q000502:physiology