Characterization of a novel exon within the D3 receptor gene giving rise to an mRNA isoform expressed in rat brain.
A novel D3 receptor cDNA containing an insertion of 84 bp has been isolated from a series of alternatively spliced D3 receptor cDNAs by polymerase chain reaction on RNA isolated from rat basal forebrain. Sequence analysis of both subcloned cDNAs and genomic DNA clones, has identified a 84 bp insertion resulting in a 28 amino acid insertion in the first extracellular loop. A comparison of the cDNA sequence of the novel D3 cDNA and the isolated genomic sequence indicates that the novel insertion corresponds to a new exon and arises via an alternative splicing event. The mRNA for the novel insertion could be detected by PCR in several areas of the brain co-localized with the wild type form of the D3 receptor. Moreover, genomic Southern blot analyses suggest that the D3-receptor exon giving rise to this novel D3 mRNA variant might exist in other species.
Pagliusi
S
S
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Chollet-Daemerius
A
A
Losberger
C
C
Mills
A
A
Kawashima
E
E
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004247:DNA; D005091:Exons; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar / Q000235:genetics*; D011954:Receptors, Dopamine / Q000096:biosynthesis* / Q000235:genetics*; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3
C493595:Drd3 protein, rat; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D004247:DNA