A TATA sequence-dependent transcriptional repressor activity associated with mammalian transcription factor IIA.
In the process of characterizing cellular proteins that modulate basal transcription by RNA polymerase II, we identified a novel repressor activity specific for promoters containing consensus TATA boxes. This activity strongly represses TATA-binding protein (TBP)-dependent transcription initiation from core promoter elements containing a consensus TATA sequence, but activates TBP-dependent transcription from core promoter elements lacking a consensus TATA sequence. Purification of this activity to near homogeneity from rat liver nuclear extracts led to the surprising discovery that it co-purifies closely with mammalian transcription factor IIA (TFIIA). The close association of TATA sequence-dependent transcriptional repressor activity with TFIIA adds a new and unexpected dimension to the already complex picture of this factor's function in transcription by RNA polymerase II.
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Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Serizawa
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Conaway
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Conaway
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JW
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D004247:DNA; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000378:metabolism*; D016385:TATA Box; D035561:Transcription Factor TFIIA; D014157:Transcription Factors / Q000378:metabolism*
D012097:Repressor Proteins; D035561:Transcription Factor TFIIA; D014157:Transcription Factors; D004247:DNA; D012319:RNA Polymerase II