Localization and functions of Japanese encephalitis virus nonstructural proteins NS3 and NS5 for viral RNA synthesis in the infected cells.
Recently it has been reported that Japanese encephalitis virus (JEV)-specific RNAs can be synthesized in vitro in the subcellular fraction including outer-nuclear membrane (Takegami and Hotta, 1989). The results of Western blot analysis and indirect immunofluorescence test using two kinds of monospecific antisera against JEV nonstructural proteins NS3 and NS5 showed that NS3 and NS5 were membrane-associated proteins and formed the complex at the perinuclear site in the infected cells. Both antisera against NS3 and NS5 inhibited in vitro RNA synthesis. These results suggest that NS5 and NS3 play important role(s) in flavivirus RNA replication.
Edward
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Division of Tropical Medicine, Kanazawa Medical University, Ishikawa, Japan.
Takegami
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T
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000276:immunology / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000276:immunology / Q000378:metabolism*; D014776:Virus Cultivation; D014779:Virus Replication
D000914:Antibodies, Viral; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase; D020365:RNA Helicases