A high performance liquid chromatographic procedure for the simultaneous determination of norfloxacin and furprofen in rat plasma.
A rapid and simple high performance liquid chromatographic analytical method is described for the simultaneous determination of norfloxacin and furprofen in rat plasma. Following dichloromethane extraction, the solution was chromatographed in a Vydac anion exchange column using a mobile phase of 0.05 M phosphate buffer (pH = 7.0): acetonitrile (80:20, v/v) at a flow-rate of 1.8 mL/min. The drugs were detected by UV absorption at 278 nm. The total chromatographic analysis time was 10 min. The response was linear, 0.1-5.0 micrograms/mL for norfloxacin and 0.1-3.0 micrograms/mL for furprofen, respectively. This method is useful for pharmacokinetic studies of these compounds and will facilitate detailed investigations into the interactions between quinolones and furprofen.
Carlucci
G
G
Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università dell'Aquila, Italy.
Mazzeo
P
P
Palumbo
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005260:Female; D008297:Male; D009643:Norfloxacin / Q000097:blood*; D010666:Phenylpropionates / Q000097:blood*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley
D010666:Phenylpropionates; D009643:Norfloxacin; C055623:furprofen