[The effect of a decrease in the mobilization of Ca+ and its chemo-controlled intake into the cytoplasm on the modulation of cholinoreceptor neuronal plasticity in Helix lucorum snails by 15-hydroxyeicosatetraenoic acid].
The effects of extracellular solution without Ca2+ and inhibitors of Ca2+ mobilization dependent of Ca2+ (tetracaine) and inositol-1,4,5-trisphosphate (TMB-8) on the modulation of cholinoreceptor (ChR) plasticity of identified Helix lucorum RPa3 and LPa3 neurons by 15(S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid (15-HETE) were investigated. Using the double-electrode voltage-clamp technique, the inward current, induced by local ionophoretic acetylcholine application (ACh-current), was recorded. ChR plasticity was evaluated by extinction of ACh-current developed in a process of rhythmic ACh applications on the soma with an interval 60-240 s. Tetracaine and TMB-8 disturbed long-latent modulatory effect of 15-HETE and did not influence short-latent one. Extraction of Ca2+ from extracellular solution did not affect the modulatory effect of 15-HETE. It was concluded that one of the molecular mechanisms of ChR plasticity deepening due 15-HETE (in long-latent phase) induces potentiation of Ca(2+)- and inositol-1,4,5-trisphosphate-dependent Ca2+ mobilization evoked by 5-lipoxygenase eicosanoids.
Pivovarov
A S
AS
Egido-Villareal
W
W
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D004347:Drug Interactions; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D006372:Helix, Snails / Q000187:drug effects* / Q000502:physiology; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D066298:In Vitro Techniques; D009473:Neuronal Plasticity / Q000187:drug effects* / Q000502:physiology; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D011950:Receptors, Cholinergic / Q000187:drug effects* / Q000502:physiology; D013748:Tetracaine / Q000494:pharmacology
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D006893:Hydroxyeicosatetraenoic Acids; D011950:Receptors, Cholinergic; D013748:Tetracaine; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D000109:Acetylcholine; D002118:Calcium
Vliianie snizheniia mobilizatsii Ca2+ i ego khemoupravliaemogo vkhoda v tsitoplazmu na moduliatsiiu plastichnosti kholinoretseptorov neĭronov vinogradnoĭ ulitki 15-gidroksiéĭkozatetraenovoĭ kislotoĭ.