The cytosolic acidification in rat parotid cells is associated with an increase in cytosolic Ca2+ concentration.
A transient decrease in cytosolic pH ([pH]i) in rat parotid cells was evoked by the addition of carbachol (CCh), phenylephrine, or substance P, whereas isoproterenol and dibutyryl cyclic AMP had little or no effect on [pH]i. The decrease in [pH]i induced by the Ca(2+)-mobilizing agonists was also observed in Ca(2+)-free medium, but not when the intracellular Ca2+ stores were previously depleted. Ionomycin and thapsigargin elicited a decrease in [pH]i with an increase in cytosolic Ca2+ concentration ([Ca2+]i). The protein kinase C activator and inhibitor had no effect on the agonist-induced decrease in [pH]i. These results suggest that the cytosolic acidification is associated with an increase in [Ca2+]i.
Tanimura
A
A
Department of Dental Pharmacology, School of Dentistry, Higashi Nippon Gakuen University, Hokkaido, Japan.
Tojyo
Y
Y
Matsumoto
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002120:Calcium Channel Agonists / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D003600:Cytosol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D015759:Ionomycin; D008297:Male; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
D002120:Calcium Channel Agonists; D013729:Terpenes; D015759:Ionomycin; D019284:Thapsigargin; D011493:Protein Kinase C; D000252:Calcium-Transporting ATPases; D002118:Calcium