Variations in the antigenic effects of tunicamycin on Candida albicans.
We report on the effect of subinhibitory doses of tunicamycin on Candida albicans cells (BP strain high responder NCYC 1466) in a defined medium favourable for expression of the mycelial phase. Tunicamycin inhibited the synthesis of some protein fractions ranging from 40 to 65 kDa, where the immunodominant antigens of C. albicans responsible for the antibody response to systemic mycosis were inhibited. By two-dimensional immunoelectrophoresis, antigen extracts from the cell cultures grown with tunicamycin showed a migration modification and a lower number of precipitation arcs with variation in their height and range.
Vespa
M N
MN
Istituto di Microbiologia, Facoltà di Farmacia, Università degli Studi di Roma, La Sapienza, Italy.
Lebecq
J C
JC
Simonetti
N
N
Bastide
J M
JM
D000946:Antigens, Fungal / Q000096:biosynthesis / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000276:immunology; D006023:Glycoproteins / Q000096:biosynthesis* / Q000276:immunology; D006031:Glycosylation / Q000187:drug effects; D007122:Immunoelectrophoresis; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D014415:Tunicamycin / Q000494:pharmacology*
D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins; D014415:Tunicamycin