title: Nutrient effects on growth and the production of 5-hydroxy-4-oxonorvaline by Streptomyces akiyoshiensis.
abstract: 5-Hydroxy-4-oxonorvaline (HON) was produced optimally by Streptomyces akiyoshiensis in media containing starch and casein supplemented with high concentrations of phosphate and magnesium sulfate; cultures grown at initial pH values between 6.3 and 6.6 yielded HON titres of 13-14 mM. Physiological analysis of HON production provided evidence that the excess inorganic constituents in this medium played an important role in optimizing production. In simple defined media buffered at pH 5.5-6.0 and inoculated with either a spore suspension or washed vegetative mycelium, formation of HON was less than one-third the amount produced in the starch-casein-salts medium. Production was markedly affected by the carbon and nitrogen sources used; media containing starch and potassium nitrate or aspartate supported the highest yields of HON. In starch-nitrate media, production was suppressed by excess nitrogen source, and the HON titre decreased 3-fold as the phosphate content was increased from 0.5 to 5 mM; growth was not appreciably altered. The results suggest that the high level of HON production in the complex starch-casein-salts medium is associated with sequestration of metabolic ammonium and reduced availability of phosphate through formation of the poorly soluble magnesium ammonium phosphate.
authors.affiliation: Biology Department, Dalhousie University, Halifax, N.S., Canada.
authors.forename: M A
authors.initials: MA
authors.lastname: Glazebrook
authors.forename: L C
authors.initials: LC
authors.lastname: Vining
authors.forename: R L
authors.initials: RL
authors.lastname: White
authors.forename: K C
authors.initials: KC
authors.lastname: Smith
authors.forename: E G
authors.initials: EG
authors.lastname: Chedrawy
mesh_terms: D000622:Aminolevulinic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D000935:Antifungal Agents / Q000096:biosynthesis*; D001224:Aspartic Acid / Q000378:metabolism; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008278:Magnesium Sulfate / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D017680:Potassium Compounds; D013213:Starch / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D009566:Nitrates; D010710:Phosphates; D017680:Potassium Compounds; D001224:Aspartic Acid; D008278:Magnesium Sulfate; C058222:2-amino-5-hydroxy-4-oxopentanoic acid; D000622:Aminolevulinic Acid; D013213:Starch; C023844:potassium nitrate