Axonal regeneration from transplanted sympathetic ganglia is not impaired by age.
Changes occur with age in several areas of the nervous system, including autonomic ganglia and the target tissues of some sympathetic neurons where signs of neuronal degeneration have been shown in old age. In this study, we have employed an intraocular grafting technique to examine whether age changes occur in sympathetic neurons of the superior cervical (SCG) and stellate ganglia that might explain the reduced innervation of some of their target tissues in old age. Ganglia taken from young and old rats were implanted in oculo for 4 or 6 weeks in young hosts whose irises had been previously sympathetically denervated. The extent, pattern, and time course of reinnervation of the host iris was studied by catecholamine histochemistry followed by image analysis. We did not observe any difference in the pattern and time course of reinnervation between ganglia taken from young and old donors. If anything, the extent of reinnervation was greater in irises implanted with SCG taken from 25-month-old animals. Moreover, we did not observe any difference in ability to reinnervate the host iris between ganglia that normally project to the iris (SCG) and ganglia that normally do not project to the iris (stellate), regardless of the age of the donor animal. Our results suggest that sympathetic neurons from aged rats have unimpaired capability of regeneration and retain plasticity, as shown by their ability to reinnervate an inappropriate target. We hypothesize that the decreased innervation observed in some sympathetic targets in old age might be caused by changes in the target tissues themselves rather than being the result of degenerative changes in the neurons.
Gavazzi
I
I
Department of Anatomy, Royal Free Hospital School of Medicine, London, U.K.
Cowen
T
T
D000375:Aging / Q000502:physiology*; D000818:Animals; D001369:Axons / Q000502:physiology*; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000502:physiology*; D007498:Iris / Q000294:innervation / Q000502:physiology; D008297:Male; D009416:Nerve Regeneration; D009417:Nerve Tissue / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley