Time-dependent mitotic recombination in Saccharomyces cerevisiae.
The time-dependent appearance of prototrophic recombinants between heterologously located artificial repeats has been studied in Saccharomyces cerevisiae. While initial prototrophic colony numbers from independent cultures were highly variable, additional recombinants were found to arise daily at roughly constant rates irrespective of culture. These late-appearing recombinants could be accounted for neither by detectable growth on the selective media nor by delayed appearance of recombinants present at the time of selective plating. Significantly, at no time did the distributions of recombinants fully match those expected according to the Luria-Delbruck model and, in fact, after the first day, the distributions much more closely approximated a Poisson distribution. Prototrophic recombinants accumulated not only on the relevant selective medium, but also on media unrelated to the acquired prototrophy.
Steele
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DF
Department of Biology, Emory University, Atlanta, GA 30322.
Jinks-Robertson
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S
D000596:Amino Acids / Q000378:metabolism; D007700:Kinetics; D008938:Mitosis; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014170:Transformation, Genetic; D014364:Tryptophan / Q000378:metabolism
D000596:Amino Acids; D014364:Tryptophan