Establishment of a novel monoclonal antibody, SE-1, which specifically reacts with rat hepatic sinusoidal endothelial cells.
A monoclonal antibody, SE-1, which specifically reacted with rat hepatic sinusoidal endothelial cells (HSE), was established with elutriator-separated HSE as immunogen. No crossreactivity was observed with other types of endothelial cells or non-endothelial cells. By immunoelectron microscopy, the antigen recognized by MAb SE-1 was localized to the membrane surface of HSE. Immunoblot analysis revealed that SE-1 recognized a single 45 KD band. Although the SE-1 antigen is not yet characterized, the above evidence strongly suggests that it is related to the specific function of HSE. Therefore, SE-1 may be a useful marker to study the role of HSE in various pathophysiological conditions of the liver.
Ohmura
T
T
Department of Pathology, Sapporo Medical College, Japan.
Enomoto
K
K
Satoh
H
H
Sawada
N
N
Mori
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000276:immunology; D004730:Endothelium, Vascular / Q000276:immunology*; D005260:Female; D008099:Liver / Q000098:blood supply*; D008970:Molecular Weight; D051381:Rats; D011916:Rats, Inbred F344
D000911:Antibodies, Monoclonal