Synthesis of 2-deoxy-D-galactose containing gangliosides in vivo.
Incorporation of 2-deoxy-D-galactose into the oligosaccharide moieties of different gangliosides of rat liver was examined. After intraperitoneal administration of 2-deoxy-D-galactose it was shown by GLC/MS analysis that this hexose analogue is metabolized and incorporated into all the gangliosides investigated, and predominantly into GM3 and GD3. In both of these gangliosides, 25-55% of the galactose residues were substituted by 2-deoxy-D-galactose. The epimer, 2-deoxy-D-glucose, was not detectable.
Hwang
Y H
YH
Institut für Molekularbiologie und Biochemie, Freien Universität Berlin, Germany.
Kohla
G
G
Geilen
C C
CC
Paul
C
C
Kannicht
C
C
Grunow
D
D
Pohlentz
G
G
Egge
H
H
Nuck
R
R
Reutter
W
W
D000818:Animals; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005679:G(M3) Ganglioside / Q000378:metabolism; D005690:Galactose / Q000031:analogs & derivatives* / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007274:Injections, Intraperitoneal; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D011915:Rats, Inbred BUF; D016339:Spectrometry, Mass, Fast Atom Bombardment
D005679:G(M3) Ganglioside; D005732:Gangliosides; C021298:ganglioside, GD1a; C016481:trisialoganglioside GT1; C026226:ganglioside, GD3; C019327:2-deoxy-lyxo-hexose; D005690:Galactose