Effect of glucose on thioltransferase activity and protein mixed disulfides concentration in GSH-depleting reagents treated rat erythrocytes.
Thioltransferase activity in rat erythrocytes is decreased and the protein mixed disulfide-concentration is increased by the treatment of t-butylhydroperoxide or diamide, significantly. Their levels are restored to their original levels very effectively by the addition of glucose.
Terada
T
T
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Nishimura
M
M
Oshida
H
H
Oshida
T
T
Mizoguchi
T
T
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D003958:Diamide / Q000494:pharmacology; D004220:Disulfides / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005215:Fasting; D005947:Glucose / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D054477:Glutaredoxins; D005978:Glutathione / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D008297:Male; D010088:Oxidoreductases / Q000097:blood*; D010545:Peroxides / Q000494:pharmacology; D011490:Protein Disulfide Reductase (Glutathione); D051381:Rats; D017208:Rats, Wistar; D020122:tert-Butylhydroperoxide
D001798:Blood Proteins; D004220:Disulfides; D054477:Glutaredoxins; D010545:Peroxides; D003958:Diamide; D020122:tert-Butylhydroperoxide; D010088:Oxidoreductases; D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase; D011490:Protein Disulfide Reductase (Glutathione); D005978:Glutathione; D005947:Glucose