Minimal residual disease in vitro of a pre-B-lymphoma.
We have studied the survival of clonogenic neoplastic cells of a murine pre-B-lymphoma (BCl-1) of the spleen in culture. We have found quantitative deficiencies such as reduced surface adherence of stromal cells and impaired CFU-F (colony forming units-fibroblast) and pre-CFU-F colony and layer formation in stromal cultures of lymphoma bearing spleen, as compared to cultures from normal spleen. There are two populations of clonogenic BCl-1 lymphoma cells surviving in culture: one population is surface adherent, and the other is non-adherent. Both populations transmit the lymphoma to healthy indicator mice. We hope that this model will be helpful in studying minimal residual leukemic disease [1] in culture.
Prindull
G
G
Department of Pediatrics, University Medical School Göttingen, Germany.
Ben-Ishay
Z
Z
Bergholz
M
M
Prindull
B
B
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival; D002999:Clone Cells; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000473:pathology; D017154:Stromal Cells / Q000473:pathology; D014407:Tumor Cells, Cultured