Crystallization of a complex between an elastase-specific inhibitor elafin and porcine pancreatic elastase.
A complex between synthetic elafin, an elastase-specific inhibitor, and porcine pancreatic elastase was crystallized using 2-methyl-2,4-pentanediol as precipitant. The crystals belong to the monoclinic space group P2(1) with cell parameters a = 37.91 A, b = 73.32 A, c = 48.92 A, beta = 105.4 degrees, and one complex molecule in the asymmetric unit. The crystals diffract X-rays to 1.9 A resolution and are suitable for crystallographic structure analysis.
Tsunemi
M
M
Peptide Institute Inc., Protein Research Foundation, Osaka, Japan.
Matsuura
Y
Y
Sakakibara
S
S
Katsube
Y
Y
D000818:Animals; D003461:Crystallography; D046911:Macromolecular Substances; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000648:ultrastructure*; D011485:Protein Binding; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors / Q000737:chemistry*; D013552:Swine; D014961:X-Ray Diffraction
D046911:Macromolecular Substances; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D010196:Pancreatic Elastase