A method to obtain large quantities of Toxoplasma gondii tachyzoites with extreme purity.
In order to extract genetic material from the intracellular parasite Toxoplasma gondii, large numbers of organisms free of host cells are necessary. A method is described in which the RH strain of T. gondii was cultivated in nonadherent U937 cells. The purification was done by countercurrent elutriation. The resulting population of 10(10) T. gondii contained only 0.17% host cell material. The method is simple, rapid, and minimizes the use of animals.
Domzig
W
W
Centre d'Immunologie et de Biotechnologie Pierre Fabre, Le Puy St. Martin, France.
Séguéla
J P
JP
Binz
H
H
D000818:Animals; D002498:Centrifugation / Q000379:methods; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse; D051379:Mice; D012692:Serial Passage; D014122:Toxoplasma / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured