Transposon-induced inversion in Antirrhinum modifies nivea gene expression to give a novel flower color pattern under the control of cycloidearadialis.
The nivea (niv) gene of Antirrhinum majus encodes chalcone synthase, an enzyme involved in synthesis of anthocyanin pigments. The nivrec:98 allele contains a single copy of the transposon Tam3 inserted at the niv locus. A large chromosomal rearrangement derived from this mutant has been shown to be flanked by two copies of Tam3. In this study, we compared sequences involved in this rearrangement with their progenitor sequences and concluded that the rearrangement is an inversion resulting from an aberrant transposition occurring shortly after replication of Tam3 that left both copies of Tam3 active after the rearrangement. Excision of Tam3 from its position adjacent to the niv coding region resulted in a novel distribution of anthocyanin pigment in the flower tube, caused by the interaction of the new sequences with the remnant of the niv promoter. The new sequences upstream of niv serve both to enhance niv transcription and to redirect the pattern of gene expression, placing niv under the control of the gene cycloidearadialis, which determines the morphogenetic polarity of the flower.
Lister
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Department of Genetics, John Innes Institute, John Innes Centre, Norwich, United Kingdom.
Jackson
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D
Martin
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C
D000483:Alleles; D000872:Anthocyanins / Q000235:genetics*; D001483:Base Sequence; D007446:Chromosome Inversion; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D017343:Genes, Plant / Q000502:physiology*; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D000872:Anthocyanins; D004251:DNA Transposable Elements