Structural studies of the Vibrio mimicus W-26768 O-antigen polysaccharide.
The structure of the Vibrio mimicus W-26768 O-antigen polysaccharide has been investigated by sugar and methylation analyses, Smith degradation, and NMR spectroscopy. It is proposed that it is composed of chains of beta-(1-->4)-linked 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-D-glucopyranosyl residues (D-Qui(p)3NR), approximately 60% of which are substituted in the 2-position with 2-acetamido-2-deoxy-alpha-D-galactopyranosyl groups. The polysaccharide does not seem to be composed of oligosaccharide repeating units but has a less regular structure, schematically indicated below. [formula: see text]
Kenne
L
L
Department of Chemistry, Swedish University of Agricultural Sciences, Uppsala.
Lindberg
B
B
Rahman
M M
MM
Mosihuzzaman
M
M
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003967:Diarrhea / Q000382:microbiology; D006801:Humans; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D014733:Vibrio / Q000737:chemistry* / Q000302:isolation & purification
D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial