Cell surface binding of colloidal iron and Limax flavus agglutinin compared by X-ray microanalysis.
The surface characteristics of tumour cells and T-cell hybridoma variants were examined by quantitative X-ray microanalysis using metal-based probes. Comparisons of populations of cells differing in invasive potential revealed differences in the binding of positive colloidal iron hydroxide, a general probe for anionic sites. Substantial differences between populations were also detected by the binding of Limax flavus agglutinin, which was used in conjunction with fetuin-gold as a specific marker for sialic acid.
Thomas
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N
Department of Biological Sciences, University of Wales, Dyfed, U.K.
Evans
P M
PM
ap Gwynn
I
I
D000818:Animals; D000838:Anions / Q000378:metabolism; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism*; D003102:Colloids; D004577:Electron Probe Microanalysis; D007501:Iron / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D019158:N-Acetylneuraminic Acid; D009361:Neoplasm Invasiveness; D037121:Plant Lectins; D011485:Protein Binding; D012794:Sialic Acids / Q000032:analysis; D014407:Tumor Cells, Cultured
D000838:Anions; D003102:Colloids; D037102:Lectins; C042962:Limax flavus agglutinin; D037121:Plant Lectins; D012794:Sialic Acids; D007501:Iron; D019158:N-Acetylneuraminic Acid