Determination of acetazolamide in dosage forms by high performance liquid chromatography.
A high performance liquid chromatographic assay for the quantitation of acetazolamide in both tablet and injection form is described. Acetazolamide is extracted with 0.005 M NaOH solution containing 0.3 mg/mL sulphadiazine (internal standard). A commercially available mu-Bondapak C18 cartridge column was used for the separation together with a mobile phase made of acetonitrile, methanol and sodium acetate buffer mixture (10:2:88) (pH 4) at a flow-rate of 4 mL/min. Retention times of about 2.50 and 3.36 min were obtained for the drug and the internal standard, respectively.
Gomaa
Z S
ZS
Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
D000086:Acetazolamide / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013607:Tablets
D013607:Tablets; D000086:Acetazolamide