title: Cloning and sequence analysis of an esterase gene from Pseudomonas sp. KWI-56.
abstract: The gene encoding an esterase from Pseudomonas sp. KWI-56 was cloned and sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide comprising 262 amino acids, whose molecular weight agreed well with the value obtained by SDS-PAGE. Comparison of the amino acid sequence with those of the other homologous enzymes suggested that Ser-92 and His-24l might be included in the catalytic triad.
authors.affiliation: Osaka Municipal Technical Research Institute, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shimada
authors.forename: T
authors.initials: T
authors.lastname: Nagao
authors.forename: A
authors.initials: A
authors.lastname: Sugihara
authors.forename: T
authors.initials: T
authors.lastname: Iizumi
authors.forename: T
authors.initials: T
authors.lastname: Yui
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Fukase
authors.forename: Y
authors.initials: Y
authors.lastname: Tominaga
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004950:Esterases / Q000737:chemistry / Q000235:genetics*; D006639:Histidine; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011549:Pseudomonas / Q000235:genetics*; D016415:Sequence Alignment; D012694:Serine
chemical_list: D012694:Serine; D006639:Histidine; D004950:Esterases