Detection of androgen receptor (AR) mRNA by the reverse transcription polymerase chain reaction (RT-PCR) in human thyroids.
In order to demonstrate that Androgen binding activity in thyroid is caused by the canonic Androgen Receptor (AR), member of steroid receptor family, we studied the presence of AR mRNA in human thyroid tissues and primary cultured cells. Here we report a polymerase chain reaction protocol (RT-PCR) that we have designated to investigate the presence of AR mRNA in human cells. AR cDNA was synthesized and amplified with primers specific for C-terminal sequence of the protein. We demonstrated that AR gene expression i) is present in thyroid samples studied and ii) in a primary culture of follicular adenoma where it seems to be modulated by steroid hormones.
Franceschetti
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P
Centro di Studi Biochimici delle Patologie del Genoma, Università degli Studi di Ferrara.
Rossi
R
R
Aguiari
G L
GL
degli Uberti
E
E
del Senno
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L
D000236:Adenoma / Q000737:chemistry / Q000473:pathology; D004247:DNA / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis; D011944:Receptors, Androgen / Q000235:genetics*; D013961:Thyroid Gland / Q000737:chemistry*; D013964:Thyroid Neoplasms / Q000737:chemistry / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011944:Receptors, Androgen; D004958:Estradiol; D014212:Tretinoin; D004247:DNA