[Inactivation of prostaglandin H synthase from sheep vesicular glands with acetylenic fatty acids].
Acetylenic fatty acids, 8,11,14-eicosatrienoic, 5,8,11,14-eicosatetraenoic and 5,8,11,14,17-eicosapentaenoic acids, were compared with respect to their effects on the particulate prostaglandin H synthase. All the acetylenic acids inactivated the enzyme in a time-dependent manner. The eicosatriynoic and eicosatetraynoic acids exhibited comparable efficacies, whereas the eicosapentaenoic acid proved to be a weaker inactivator. The time-courses of inactivation fitted a kinetic model suggest an enzyme-catalysed conversion of the compounds via alternative suicidal and non-suicidal routes. The characteristics of inactivation of prostaglandin H synthase differ from those of lipoxygenases.
Sheve
Kh
Kh
Belosludtsev
Iu Iu
IuIu
Demin
P M
PM
Khol'tskhiutter
Kh G
KhG
Sheve
T
T
Miakova
G I
GI
Evstigneeva
R P
RP
D000818:Animals; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D015777:Eicosanoids / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000302:isolation & purification*; D012669:Seminal Vesicles / Q000201:enzymology*; D012756:Sheep
D016861:Cyclooxygenase Inhibitors; D015777:Eicosanoids; D011451:Prostaglandin-Endoperoxide Synthases
Inaktivatsiia prostaglandin-H-sintazy iz vezikuliarnykh zhelez barana atsetilenovymi zhirnymi kislotami.