Measurement of eicosanoids in menstrual fluid by the combined use of high pressure chromatography and radioimmunoassay.
Methods are described for the quantification of various eicosanoids (cyclooxygenase products: 6-KETO, TXB2, PGE2, PGF2 alpha, DHK; lipoxygenase products: 5-, 12-, 15-HETE, LTB4, LTC4, LTD4, LTE4) in menstrual blood collected by tampons. Samples were extracted with acidified ethanol. After purification by SEP-PACK C18 columns, the compounds were separated by reversed phase HPLC using a ternary gradient system. The eicosanoid concentrations of the fractionated eluents were measured by radioimmunoassay and corrected for recovery. 12-HETE was the most prominent metabolite of arachidonic acid in menstrual blood (mean: 1174 ng/g blood). With the exception of PGF2 alpha and TXB2 (mean: 343 and 212 ng/g blood, respectively) other eicosanoids were detected in remarkable lower concentrations.
Hofer
G
G
Second Department of Obstetrics and Gynecology, University Hospital of Vienna, Austria.
Bieglmayer
C
C
Kopp
B
B
Janisch
H
H
D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015777:Eicosanoids / Q000032:analysis*; D005260:Female; D006801:Humans; D008084:Lipoxygenase / Q000378:metabolism; D052576:Menstrual Hygiene Products; D008598:Menstruation / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011863:Radioimmunoassay
D015777:Eicosanoids; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases