title: A manifold support for molecular genetic reactions.
abstract: Large numbers of samples can be efficiently processed through sequential reaction steps using a 96-pronged support that projects into individual microtiter wells. The support was constructed by creating a porous surface on a disposable polystyrene manifold, with avidin coupled to this greatly expanded surface. The shape and high binding capacity of the device allow the parallel transfer of large sets of reaction intermediates between different binding or enzymatic processing steps. We have applied the support to increase the efficiency of preparative and analytical molecular genetic reactions. The support also reduce the risks of sample mix-up and contamination in applications such as PCR and DNA sequencing.
authors.affiliation: Department of Medical Genetics, Uppsala Biomedical Center, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Parik
authors.forename: M
authors.initials: M
authors.lastname: Kwiatkowski
authors.forename: A
authors.initials: A
authors.lastname: Lagerkvist
authors.forename: M
authors.initials: M
authors.lastname: Lagerström Fermér
authors.forename: M
authors.initials: M
authors.lastname: Samiotaki
authors.forename: J
authors.initials: J
authors.lastname: Stewart
authors.forename: G
authors.initials: G
authors.lastname: Glad
authors.forename: M
authors.initials: M
authors.lastname: Mendel-Hartvig
authors.forename: U
authors.initials: U
authors.lastname: Landegren
mesh_terms: D001360:Avidin; D001426:Bacterial Proteins; D001710:Biotin; D004247:DNA / Q000302:isolation & purification; D007700:Kinetics; D008967:Molecular Biology / Q000295:instrumentation*; D016133:Polymerase Chain Reaction; D011137:Polystyrenes; D012685:Sepharose; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D011137:Polystyrenes; D001360:Avidin; D001710:Biotin; D004247:DNA; D012685:Sepharose; D019809:Streptavidin