title: Alterations induced by Trypanosoma cruzi in activated mouse lymphocytes.
abstract: Although a number of immunological anomalies have been shown to occur during the acute period of Trypanosoma cruzi infection, the contribution of the parasite has not been clarified. In this work, we co-cultured activated splenic mononuclear cells (SMC) from normal outbred (CD1) or inbred (CBA/J) mice with purified T. cruzi trypomastigotes and studied ensuing T- and B-lymphocyte alterations. In the presence of parasites, phytohaemagglutinin-stimulated SMC from either mouse background manifested a marked reduction in both lymphoproliferative capacity (i.e., 3H-thymidine incorporation) and cell membrane level of interleukin-2 receptors (IL-2R; determined by flow cytometry) relative to SMC from parasite-free cultures. Thus, substantial proportions of activated SMC either became unable to express detectable levels of IL-2R or expressed this receptor in significantly lower numbers than control SMC. Supernatants from T. cruzi suspensions reproduced these suppressive effects on phytohaemagglutinin-stimulated SMC from normal or chronically infected CD1 or CBA/J mice. Similar results were obtained with SMC activated with a bacterial lipopolysaccharide. Since IL-2R expression is required for activated lymphocytes to progress through the cell cycle and multiply to mount effective immune responses, impaired IL-2R expression by T. cruzi provides a plausible hypothesis for the wide-ranged immunosuppression that occurs in the infected host.
authors.affiliation: Department of Microbiology and Public Health, Michigan State University, East Lansing 48823.
authors.forename: H M
authors.initials: HM
authors.lastname: Lopez
authors.forename: M K
authors.initials: MK
authors.lastname: Tanner
authors.forename: F
authors.initials: F
authors.lastname: Kierszenbaum
authors.forename: M B
authors.initials: MB
authors.lastname: Sztein
mesh_terms: D000818:Animals; D002455:Cell Division; D014355:Chagas Disease / Q000276:immunology* / Q000469:parasitology; D002908:Chronic Disease; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007166:Immunosuppressive Agents / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D008813:Mice, Inbred ICR; D008934:Mitogens / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D007166:Immunosuppressive Agents; D008934:Mitogens; D015375:Receptors, Interleukin-2