Effect of cationization on anti-hapten antibody responses in sheep and mice.
The effect on anti-hapten immune responses of altering the isoelectric point of hapten-carrier conjugates by cationization was examined in sheep and mice. No adjuvants were employed in these studies. In sheep the cationization of ovalbumin-dinitrophenyl (OVA-DNP) resulted in an enhanced primary anti-dinitrophenyl (DNP) response. The secondary anti-DNP responses were not consistently greater for animals primed with cationized (CAT) OVA-DNP than in animals primed with OVA-DNP. Similar results were obtained with mice immunized with bovine serum albumin (BSA)-DNP or CAT BSA-DNP. Mice and sheep primed with OVA-DNP failed to mount an anamnestic response following secondary immunization with CAT OVA-DNP. Failure of a cationized hapten-carrier to stimulate a secondary anti-DNP response following priming with uncationized hapten-carrier was also observed in mice using BSA-DNP as the model antigen.
Altmann
K G
KG
CSIRO, Division of Animal Health, Armidale, NSW, Australia.
D000818:Animals; D000917:Antibody Formation; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens / Q000276:immunology*; D007114:Immunization; D007115:Immunization Schedule; D007132:Immunoglobulin Isotypes / Q000032:analysis; D007526:Isoelectric Point; D051379:Mice; D010047:Ovalbumin / Q000031:analogs & derivatives / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology*; D012756:Sheep
D006241:Haptens; D007132:Immunoglobulin Isotypes; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D004139:Dinitrofluorobenzene