title: Regulation of glucocorticoid receptor function through assembly of a receptor-heat shock protein complex.
abstract: Incubation of immunopurified, hormone-free mouse glucocorticoid receptors with rabbit reticulocyte lysate results in ATP-dependent and monovalent cation-dependent assembly of the GR into a heterocomplex with hsp90, hsp70, and hsp56. Heterocomplex assembly is accompanied by conversion of the receptor from a form that does not bind steroid to a high affinity steroid-binding conformation. Reticulocyte lysate also promotes ATP-dependent dissociation of unliganded receptors from a prebound receptor-DNA complex. Receptor released from DNA has been reconstituted into the heat shock protein heterocomplex and converted to the non-DNA-binding state. The reticulocyte lysate also reconstitutes pp60v-src into a heterocomplex containing hsp90 and p50, both of which are components of the native heterocomplex form of the tyrosine kinase in cytoplasm. Although the c-Raf-1 serine/threonine kinase has never been found in native association with hsp90, it can be assembled into a heat shock protein heterocomplex by the ATP-dependent system in reticulocyte lysate.
authors.affiliation: Department of Pharmacology, University of Michigan Medical School, Ann Arbor 48109.
authors.forename: K A
authors.initials: KA
authors.lastname: Hutchison
authors.forename: L C
authors.initials: LC
authors.lastname: Scherrer
authors.forename: M J
authors.initials: MJ
authors.lastname: Czar
authors.forename: L F
authors.initials: LF
authors.lastname: Stancato
authors.forename: Y H
authors.initials: YH
authors.lastname: Chow
authors.forename: R
authors.initials: R
authors.lastname: Jove
authors.forename: W B
authors.initials: WB
authors.lastname: Pratt
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D051379:Mice; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism* / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D011965:Receptors, Glucocorticoid; D004247:DNA