title: The effect of commercial processing on the paralytic shellfish poison (PSP) content of naturally-contaminated Acanthocardia tuberculatum L.
abstract: A study was undertaken to determine if any reduction in contamination of Acanthocardia tuberculatum L. (Mediterranean cockle) by paralytic shellfish poisons (PSP) could be enhanced by operations carried out during the industrial canning process, allowing contaminated raw material to be commercially marketed in safe conditions for edible purposes. A general decrease in PSP levels was consistently observed when comparing raw materials and their corresponding final products, these dropping to acceptable levels. PSP levels were determined by mouse bioassay and a fluorometric method, and saxitoxin was determined by HPLC. The detoxifying effects averaged over 71.7% and 81.8% (mouse bioassay), 70.6% and 90.9% (fluorometric method), 77.9% and 83.5% (HPLC), for boiling and sterilizing operations respectively. The highest level detected in raw material was 800 micrograms/100 g by mouse bioassay.
authors.affiliation: Centro Nacional de Alimentación, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Berenguer
authors.forename: L
authors.initials: L
authors.lastname: Gonzalez
authors.forename: I
authors.initials: I
authors.lastname: Jimenez
authors.forename: T M
authors.initials: TM
authors.lastname: Legarda
authors.forename: J B
authors.initials: JB
authors.lastname: Olmedo
authors.forename: P A
authors.initials: PA
authors.lastname: Burdaspal
mesh_terms: D000818:Animals; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D005511:Food Handling; D005519:Food Preservation; D008387:Marine Toxins / Q000032:analysis*; D051379:Mice; D008974:Mollusca / Q000737:chemistry*; D012530:Saxitoxin / Q000032:analysis; D012758:Shellfish; D013030:Spain
chemical_list: D008387:Marine Toxins; D012530:Saxitoxin