The CD8 alpha locus is located on the telomere side of the immunoglobulin kappa locus at a distance of 2 Mb.
The immunoglobulin kappa and CD8 alpha loci had been mapped by in situ hybridization to the short arm of chromosome 2 at 2cen-p11.2 and 2p1, respectively, but probes derived from the two loci did not hybridize to the same large PFGE fragments. We have now succeeded in linking the two loci on a 3.0-Mb fragment from a partial NruI digest. A new hybridization probe located between the two loci provided additional linking fragments and allowed the distance to be determined as 2.0-2.2 Mb.
Weichhold
G M
GM
Institut für Physiologische Chemie, Universität München, Germany.
Huber
C
C
Parnes
J R
JR
Zachau
H G
HG
D016827:CD8 Antigens / Q000235:genetics*; D002460:Cell Line; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D005796:Genes; D005803:Genes, Immunoglobulin; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008214:Lymphocytes; D014407:Tumor Cells, Cultured
D016827:CD8 Antigens; D007145:Immunoglobulin kappa-Chains; C055654:endodeoxyribonuclease NruI; D015252:Deoxyribonucleases, Type II Site-Specific