title: Molecular action of the type 1 ribosome-inactivating protein saporin 5 on Vicia sativa ribosomes.
abstract: The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fragment of approximately 370 nucleotides the 5'-end sequence of which was 5'-GAGGAACG-3'.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Valladolid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Iglesias
authors.forename: F J
authors.initials: FJ
authors.lastname: Arias
authors.forename: M A
authors.initials: MA
authors.lastname: Rojo
authors.forename: C
authors.initials: C
authors.lastname: Escarmis
authors.forename: J M
authors.initials: JM
authors.lastname: Ferreras
authors.forename: T
authors.initials: T
authors.lastname: Girbés
mesh_terms: D000818:Animals; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D000922:Immunotoxins; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000494:pharmacology*; D010944:Plants; D012335:RNA, Ribosomal; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism; D000076985:Saporins
chemical_list: D000922:Immunotoxins; D010940:Plant Proteins; D012335:RNA, Ribosomal; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins