cDNA cloning of a mouse factor that activates transcription from a metal response element of the mouse metallothionein-I gene in yeast.
A cDNA that encodes a mouse factor that activates expression from a metal response element of the mouse metallothionein-I gene has been isolated by complementation cloning in yeast cells. The cDNA encodes a peptide with a maximum length of 99 amino acids that includes a single zinc finger sequence. In yeast cells, the cloned factor induces transcription from the metal response element in a sequence-specific but metal-independent fashion. The cDNA hybridizes to a 550-base mRNA that is constitutively expressed in mouse tissue culture cells. The ability of the mouse factor to activate transcription in yeast cells is dependent upon the carbon source.
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Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D007739:L Cells; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae; D015534:Trans-Activators / Q000235:genetics*
D004268:DNA-Binding Proteins; C082192:Polr2k protein, mouse; D015534:Trans-Activators; D008668:Metallothionein