title: A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution.
abstract: One of the facets of the host defense of higher insects is the rapid and transient synthesis, following bacterial challenge or trauma, of a battery of potent antibacterial peptides (Steiner, H., Hultmark, D., Engström, A., Bennich, H., and Boman, H. G. (1981) Nature 292, 246-248). The best characterized of these peptides are the cecropins (ibid.), 4-kDa peptides devoid of cysteines, and the insect defensins (Hoffmann, J. A., and Hetru, C. (1992) Immunol. Today 13, 411-415), 4-kDa peptides with three intramolecular disulfide bridges. Several other inducible antibacterial peptides have been characterized only at the level of their amino acid sequences (Hoffmann, J. A., Dimarcq, J. L., and Bulet, P. (1992) Médecine & Sciences 8, 432-439). We report here the isolation of a novel 19-residue proline-rich inducible antibacterial peptide from Drosophila. In contrast to all previous reports on antibacterial peptides, this molecule carries a substitution as evidenced by molecular mass determinations; our data show that this reflects the O-glycosylation of a Thr residue by an N-acetylgalactosamine plus a galactose. A synthetic nonsubstituted peptide of identical amino acid sequence has an activity several times lower (5-10) than the native compound. Our data suggest that this substitution represents a post-translational modification essential for the full biological activity of this novel peptide.
authors.affiliation: Laboratoire de Biologie Générale, Université Louis Pasteur, Unité de Recherche Associée (URA) Centre National de la Recherche Scientifique (CNRS) 1490, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Bulet
authors.forename: J L
authors.initials: JL
authors.lastname: Dimarcq
authors.forename: C
authors.initials: C
authors.lastname: Hetru
authors.forename: M
authors.initials: M
authors.lastname: Lagueux
authors.forename: M
authors.initials: M
authors.lastname: Charlet
authors.forename: G
authors.initials: G
authors.lastname: Hegy
authors.forename: A
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: J A
authors.initials: JA
authors.lastname: Hoffmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001483:Base Sequence; D002241:Carbohydrates / Q000032:analysis; D003001:Cloning, Molecular; D004247:DNA; D004330:Drosophila; D004926:Escherichia coli / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006020:Glycopeptides / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D006031:Glycosylation; D008969:Molecular Sequence Data
chemical_list: D000900:Anti-Bacterial Agents; D002241:Carbohydrates; D006020:Glycopeptides; C082117:drosocin; D004247:DNA