Immunohistochemical study of estrogen receptors and the responsiveness to estrogen in papillary thyroid carcinoma.
BACKGROUND
Epidemiologic data suggested that the differentiated thyroid carcinoma was affected by sex hormones, especially estrogen.


METHODS
The authors have immunohistochemically studied estrogen receptors (ER) in papillary thyroid carcinoma in women. Two anti-ER monoclonal antibodies, D5 and H222, were used. The authors then investigated the responsiveness to estrogen of thyroid neoplasms using primary culture of surgically resected thyroid tissues. The proliferative activity of cultured cells was measured by the bromodeoxyuridine (BrdU) labeling index (LI).


RESULTS
Twenty-six percent of the specimens (18/70) showed positive staining with D5, but only 5.6% of the specimens (2/36) showed positive staining with H222. The BrdU LI of ER-positive papillary thyroid carcinoma increased with the addition of estradiol.


CONCLUSIONS
These findings indicate that estrogen may affect the proliferation of ER-positive papillary thyroid carcinoma.
Inoue
H
H
Second Department of Surgery, School of Medicine, University of Tokushima, Japan.
Oshimo
K
K
Miki
H
H
Kawano
M
M
Monden
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D002291:Carcinoma, Papillary / Q000737:chemistry* / Q000188:drug therapy; D002478:Cells, Cultured; D002648:Child; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000032:analysis*; D013964:Thyroid Neoplasms / Q000737:chemistry* / Q000188:drug therapy
D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen; D004958:Estradiol; D001973:Bromodeoxyuridine