Determination of ascorbic acid by isotachophoresis with regard to its potential in neuroblastoma therapy.
Analytical capillary isotachophoresis was used to determine ascorbic acid (AA) in different matrices (cell-free system, neuroblastoma cell extracts and urine). The system for purging bone marrow of neuroblastoma cells, including 6-hydroxydopamine (6-OHDA) and AA, was analysed with regard to the interaction of AA with 6-OHDA and its autoxidation product, hydrogen peroxide. Furthermore, analyses concerning the uptake of AA into neuroblastoma cells as well as its excretion in urine after uptake of large amounts were carried out.
Gebhardt
S
S
Children's Hospital, University of Tübingen, Germany.
Kraft
K
K
Lode
H N
HN
Niethammer
D
D
Schmidt
K H
KH
Bruchelt
G
G
D001205:Ascorbic Acid / Q000737:chemistry* / Q000627:therapeutic use*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004586:Electrophoresis; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D009447:Neuroblastoma / Q000737:chemistry* / Q000188:drug therapy*; D016627:Oxidopamine / Q000737:chemistry; D010101:Oxygen Consumption; D014407:Tumor Cells, Cultured
D016627:Oxidopamine; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid