A new form of tumor and fetal collagen that binds laminin.
Human breast and colon carcinoma tissues contain a form of collagen, not described before, composed of alpha 1 chains of similar size (approximately 100 kDa) but different charge. The three constitutive chains, separated by two-dimensional electrophoresis, are a unique acidic component, undetectable in other collagen types, with an apparent isoelectric point of 4-5, and two more basic components displaying the same electrophoretic behavior as alpha 1(III) and alpha 1(I), respectively. The acidic chain is structurally distinct from alpha 1(I) and displays a cyanogen bromide-derived fragment of similar size to CB5(III). This collagen in its native state is resistant to trypsin and metalloproteinase 3, while it is fully degraded by metalloproteinases 1 and 9. Moreover, this collagen appears able to bind to laminin, as tested by affinity chromatography. The biological significance of our data is related to the finding of this collagen form not only in the tumor tissue tested but also in embryonic-fetal tissues (bovine skin and intestine and human umbilical cord). For its peculiar laminin-binding ability and occurrence in tumoral and embryonic-fetal tissues, we propose to temporarily term this new collagen form OF/LB collagen (onco-fetal, laminin-binding collagen). The presence of OF/LB collagen during development and cancer, and its absence in normal adult tissues, make this protein a potential stromal marker of malignancy.
Pucci-Minafra
I
I
Dipartimento di Biologia Cellulare e dello Sviluppo, Università, Palermo, Italy.
Luparello
C
C
Andriolo
M
M
Basiricò
L
L
Aquino
A
A
Minafra
S
S
D000818:Animals; D001943:Breast Neoplasms / Q000737:chemistry*; D002417:Cattle; D003094:Collagen / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D003110:Colonic Neoplasms / Q000737:chemistry*; D003488:Cyanogen Bromide; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000737:chemistry / Q000196:embryology; D007526:Isoelectric Point; D007797:Laminin / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism; D008854:Microscopy, Electron; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012867:Skin / Q000737:chemistry / Q000196:embryology; D014357:Trypsin / Q000378:metabolism; D014470:Umbilical Cord / Q000737:chemistry
D007797:Laminin; D010446:Peptide Fragments; D003094:Collagen; D014357:Trypsin; D008666:Metalloendopeptidases; D003488:Cyanogen Bromide