A murine homolog of the yeast RNA1 gene is required for postimplantation development.
A gene has been characterized that is required for postimplantation mouse development. The gene, designated fug1, was disrupted in embryonic stem cells by the U3Neo gene trap retrovirus, and the disrupted allele was introduced into the germ line. Homozygous mutant embryos arrest at the egg cylinder stage at about embryonic day 6 and are mostly resorbed by day 8.5. The appearance of the proamniotic cavity is delayed, and epiblast cells that surround the cavity are disorganized. fug1 transcripts are undetectable at E6 but are induced throughout the embryo after E6.5. The gene is expressed at low levels in all adult tissues examined, maps to chromosome 15, and is conserved among mammals. The cDNA sequence encodes a protein of 589 amino acids, the first 400 of which are 38% identical to the Saccaromyces cerevisiae RNA1 gene. Regions of greatest similarity include a long acidic domain and 11 leucine-rich motifs, thought to mediate high affinity protein-protein interactions. These similarities suggest that Fug1 may be required for developmental changes in RNA processing or chromatin structure prior to gastrulation.
DeGregori
J
J
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2363.
Russ
A
A
von Melchner
H
H
Rayburn
H
H
Priyaranjan
P
P
Jenkins
N A
NA
Copeland
N G
NG
Ruley
H E
HE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D002472:Cell Transformation, Viral; D017124:Conserved Sequence; D004475:Ectoderm / Q000166:cytology; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics; D020690:GTPase-Activating Proteins; D005775:Gastrula; D015870:Gene Expression; D005796:Genes; D005800:Genes, Fungal; D005804:Genes, Lethal; D018095:Germ-Line Mutation; D017403:In Situ Hybridization; D007930:Leucine / Q000032:analysis; D008297:Male; D051379:Mice / Q000196:embryology* / Q000235:genetics; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011506:Proteins / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D005656:Fungal Proteins; D020690:GTPase-Activating Proteins; D011506:Proteins; D012367:RNA, Viral; C060997:RNA1 protein, S cerevisiae; C085844:Rangap1 protein, mouse; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D007930:Leucine