Transforming growth factor-beta 1 is chemotactic for interleukin-2-activated natural killer cells.
We examined the effect of transforming growth factor-beta 1 (TGF-beta 1) on the in vitro motility of interleukin-2-activated natural killer (IANK) cells. Low doses of TGF-beta 1 (0.01 or 0.1 ng/ml) are chemotactic for these cells as determined by modified Boyden chamber assay. IANK cells bind biotinylated TGF-beta suggesting that they express receptors for this cytokine. TGF-beta 1-induced IANK cell chemotaxis was inhibited by protein kinase C inhibitors, such as staurosporine and H7. The role of calcium mobilization in TGF-beta 1 activity was also examined; the inhibitor of intracellular Ca2+, TMB-8, inhibited TGF-beta 1-induced IANK cell chemotaxis. Supporting the role for Ca2+ mobilization is the ability of low doses of TGF-beta 1 to induce the recruitment of intracellular Ca2+ as determined by a fura-2-AM-loaded-cell assay.
Maghazachi
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Northeastern Ontario Regional Cancer Centre, Sudbury, Canada.
al-Aoukaty
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D000818:Animals; D002118:Calcium / Q000502:physiology; D002630:Chemotactic Factors / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects*; D008297:Male; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D002630:Chemotactic Factors; D007376:Interleukin-2; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D011493:Protein Kinase C; D002118:Calcium