title: 1.5-Mb YAC contig in Xq28 formatted with sequence-tagged sites and including a region unstable in the clones.
abstract: A contig of 20 yeast artificial clones (YACs) has been assembled across 1.5 Mb of Xq28 and formatted with nine previously reported probes and nine STSs developed from the sequence of probes and end fragments of YACs. YAC end fragments were obtained by subcloning, Alu-vector PCR, or primer-ligation PCR methods. Eighteen of the YACs were recovered from a library specific for Xq24-q28; two that fill a gap were obtained from a second library made from total human DNA. One region, containing probes pX78c and 2A1.1, was unstable in YACs, but it was possible to generate a self-consistent map of DNA over the entire contig. Overlaps were confirmed by Southern blot analyses of YAC DNAs, and pulsed-field gel electrophoresis confirmed the extent of the contig and identified at least four CpG islands in the region.
authors.affiliation: International Institute of Genetics and Biophysics, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Palmieri
authors.forename: G
authors.initials: G
authors.lastname: Romano
authors.forename: A
authors.initials: A
authors.lastname: Casamassimi
authors.forename: M
authors.initials: M
authors.lastname: D'Urso
authors.forename: R D
authors.initials: RD
authors.lastname: Little
authors.forename: F E
authors.initials: FE
authors.lastname: Abidi
authors.forename: D
authors.initials: D
authors.lastname: Schlessinger
authors.forename: M
authors.initials: M
authors.lastname: Lagerström
authors.forename: H
authors.initials: H
authors.lastname: Malmgren
authors.forename: M L
authors.initials: ML
authors.lastname: Steen-Bondeson
mesh_terms: D001483:Base Sequence; D015825:Chromosomes, Fungal; D002877:Chromosomes, Human; D003001:Cloning, Molecular; D004247:DNA; D016521:Electrophoresis, Gel, Pulsed-Field; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016324:Sequence Tagged Sites; D014960:X Chromosome
chemical_list: D004247:DNA