No evidence of oxidant stress during high-intensity rowing training.
In untrained subjects, strenuous exercise provokes the appearance of oxidant stress markers in blood and muscle. On the other hand, trained muscle is resistant to oxidant stress unless exercise challenges the muscle glycogen supply. It is not known whether chronic high-intensity exercise alters the susceptibility of skeletal muscle to oxidant stress, whether there are gender-related differences in markers of oxidant stress, or whether elevating muscle glycogen stores by increasing dietary carbohydrate can minimize any exercise-related oxidant stress. To address these issues, collegiate rowers (12 men, 11 women) were randomly assigned to a moderate-(MOD, 5 g/kg body wt) or high-carbohydrate (HI, 10 g/kg) diet in a double-blind design and underwent strenuous training for 4 wk. Training in the A.M. was 40 min at 70% maximal O2 consumption (VO2); in the P.M. it was either three 2,500-m time trials (to assess power output) or aerobic and lactate tolerance training. Total daily training time was 65 min at 70% maximal VO2 and 38 min at > or = 90% maximal VO2. Thrice-weekly morning blood samples were assayed for serum creatine kinase (CK), plasma thiobarbituric acid-reactive substances (TBARS), and serum beta-glucuronidase (beta-Gluc). Weekly muscle biopsies were obtained for analysis of glycogen and, when tissue sample quantity allowed, TBARS. HI rowers produced more power and improved power more (10.7 +/- 1.0 vs. 1.6 +/- 1.6%) over the 4 wk than did the MOD rowers. Preexercise muscle glycogen concentration was maintained at 119 mmol/kg in MOD but increased 65% in HI rowers (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Dernbach
A R
AR
Exercise Physiology Laboratory, School of Health, Physical Education, and Recreation, Ohio State University, Columbus 43210-1284.
Sherman
W M
WM
Simonsen
J C
JC
Flowers
K M
KM
Lamb
D R
DR
D000328:Adult; D001265:Athletic Injuries / Q000503:physiopathology / Q000517:prevention & control; D015415:Biomarkers; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D003402:Creatine Kinase / Q000097:blood; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004311:Double-Blind Method; D005260:Female; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D016877:Oxidants / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology; D010806:Physical Education and Training; D012727:Sex Characteristics; D013312:Stress, Physiological / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D015415:Biomarkers; D004040:Dietary Carbohydrates; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; D006003:Glycogen; D003402:Creatine Kinase