Recombinant interferon-gamma enhances resistance to acute disseminated Candida albicans infection in mice.
The effect of recombinant rat interferon-gamma (rIFN-gamma) on acute disseminated Candida albicans infection in mice was investigated. Outgrowth of C. albicans in kidneys, spleen, and liver of mice treated with one intravenous (iv) dose of rIFN-gamma before iv injection of 5 x 10(5) cfu of C. albicans was significantly lower than in controls over 7 days. rIFN-gamma was protective when given 1 day before, simultaneously with, or 1-3 days after infection but not when given 3 days before. In mice pretreated with hydrocortisone acetate, rIFN-gamma significantly reduced the outgrowth only when 10(3) cfu of C. albicans was injected. Injection of rIFN-gamma did not reduce the outgrowth of C. albicans in cyclophosphamide-pretreated mice and significantly increased the capacity of peripheral blood and exudate peritoneal granulocytes to kill C. albicans in vitro. Thus, rIFN-gamma enhances host resistance against acute disseminated C. albicans infection in mice through activation of polymorphonuclear leukocytes.
Kullberg
B J
BJ
Department of Infectious Diseases, University Hospital Leiden, Netherlands.
van 't Wout
J W
JW
Hoogstraten
C
C
van Furth
R
R
D000704:Analysis of Variance; D000818:Animals; D001772:Blood Cell Count / Q000187:drug effects; D016466:CHO Cells; D002176:Candida albicans / Q000302:isolation & purification; D002177:Candidiasis / Q000276:immunology* / Q000517:prevention & control; D006224:Cricetinae; D003520:Cyclophosphamide / Q000494:pharmacology; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology; D007113:Immunity, Innate; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000494:pharmacology*; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D009504:Neutrophils / Q000276:immunology; D051381:Rats; D011994:Recombinant Proteins; D013154:Spleen / Q000382:microbiology
D011994:Recombinant Proteins; D007371:Interferon-gamma; D003520:Cyclophosphamide; D006854:Hydrocortisone