[Prognostic value of nuclear DNA of 106 colorectal tumors, determined with microspectrocytophotometry].
OBJECTIVE
The aim of this study was to look for correlations between nuclear DNA content of colo-rectal tumors, and such clinical parameters as age, sex, location, CEA, histological grade and Duke's stages.


METHODS
A prospective study is carried out on surgical patients, subjected to standard criteria of radicality. Nuclear DNA content was quantified in tumoral cells by microcytophotometric techniques.


METHODS
106 patients with colo-rectal cancer. Patients with colonic perforation, other concomitant neoplasia, non-curative surgery or receiving adjuvant therapies were excluded from the study. Five patients died during the postoperative period and one was lost.


RESULTS
Histological grade: 28% G1, 35% G2 and 37% G3. Dukes': 8% A, 40% B, 32% C and 20% D. DNA quantification has rendered 45% as euploid and 55% as aneuploid. There was no statistical correlation between ploidy and location, age, sex or CEA. However, there is a clear preponderance of euploid tumors in G1 (23 vs. 5), while the aneuploid tumors double the euploid ones (24 and 25 vs. 12 and 12) in G2 and G3. A similar result was found comparing ploidy and Dukes: euploid tumors reach 77% both in stages A and B, while they drop to 24% and 14% in stages C and D. It has also been found that euploid tumors show a longer period of survival free of recurrence.


CONCLUSIONS
Evidence has been found supporting a prognostic value for tumoral DNA quantification in colo-rectal cancer. A longer follow-up is required to study absolute survival of the patients.
Díaz Aguirregoitia
F J
FJ
Departamento de Cirugía, Radiología y Medicina Física, Hospital de Cruces (Servicio de Cirugía A).
García-Alonso Montoya
I
I
Iturburu Belmonte
I
I
Portugal Porras
V
V
Alonso Varona
A
A
Echevarría García-Sanfrechoso
A
A
Méndez Martín
J
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D003592:Cytophotometry; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D015591:Microspectrophotometry; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D011446:Prospective Studies; D012004:Rectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology
D004273:DNA, Neoplasm
Valor pronóstico del ADN nuclear de 106 tumores colorrectales, medido por microespectrocitofotometría.