Depletion of the monoamino metabolites of Zoalene during frozen storage.
3-Amino-5-nitro-o-toluamide and 5-amino-3-nitro-o-toluamide, the principal metabolites in the tissues of chickens fed a diet containing Zoalene (3,5-dinitro-o-toluamide), were shown to deplete in frozen liver tissues stored up to 1 year at -20 degrees C, but not at -70 degrees C. A slight loss of 5-amino-3-nitro-o-toluamide also occurred in thigh muscle at -20 degrees C. Evidence is presented that demonstrates that the depletion of the metabolites was partially the result of their transformation to glucopyranosyl derivatives; both the alpha- and beta-anomers of the 5-amino-3-nitro-o-toluamide conjugate were observed in liquid chromatograms of tissue extracts.
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U.S. Department of Agriculture, Eastern Regional Research Center, Philadelphia, PA 19118.
D000818:Animals; D002645:Chickens; D004135:Dinitolmide / Q000032:analysis*; D005504:Food Analysis; D005519:Food Preservation; D005615:Freezing; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis; D009132:Muscles / Q000737:chemistry
D004135:Dinitolmide