Synergy of IL-7 and v-Ha-ras in the in vitro neoplastic progression of murine pre-B cells.
A recombinant retrovirus expressing IL-7 was superinfected into a murine pre-B cell line previously infected with a retrovirus expressing v-Ha-ras. Populations of cells polyclonal for integration of the IL-7 virus and independent of IL-7 for growth were generated. These cells were highly tumorigenic. Similarly, co-infection of murine bone marrow with retroviruses expressing IL-7 and v-Ha-ras generated a pre-B lymphoid outgrowth that was tumorigenic. While IL-7 and v-Ha-ras expression are not individually sufficient to induce tumorigenicity, their co-expression yields highly tumorigenic pre-B lymphoid cell lines. IL-7 expression may be equivalent to v-myc expression for synergy with v-H-ras in the neoplastic transformation of murine pre-B cells.
Chen
S C
SC
Department of Microbiology, Michigan State University, East Lansing 48824-1101.
Redenius
D
D
Young
J C
JC
Schwartz
R C
RC
D016475:3T3 Cells; D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology*; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D011905:Genes, ras; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D015851:Interleukin-7 / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C
D015851:Interleukin-7