Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum.
Protoplasts of a pAN7-1-transformed isolate of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences and the expression of the hph gene was studied in 85 progeny. A very high proportion (80%) of the progeny contained vector sequences and 70% of the progeny were resistant to hygromycin. Inactivation of the hph gene occurred in eight progeny. Rearrangement of vector sequences can account for the inactivation: there is no evidence for any RIP-like mechanism. A number of novel bands were observed.
Arnau
J
J
School of Biological Sciences, University of East Anglia, Norwich, UK.
Oliver
R P
RP
D015139:Blotting, Southern; D002934:Cinnamates; D002963:Cladosporium / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D004274:DNA, Recombinant / Q000235:genetics; D005822:Genetic Vectors; D006238:Haploidy; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D011523:Protoplasts / Q000378:metabolism; D015183:Restriction Mapping; D014170:Transformation, Genetic
D002934:Cinnamates; D004274:DNA, Recombinant; D006921:Hygromycin B; C026273:hygromycin A