Immunohistochemical localization of a neuron-specific beta-tubulin isotype in the developing chicken ultimobranchial glands.
The localization of a neuron-specific beta-tubulin isotype in the ultimobranchial glands from chickens at various stages of development was studied by means of light- and electron microscopic immunohistochemistry with a monoclonal antibody (TuJ1) against the beta-tubulin isotype, c beta 4. At 8 days of incubation, many C cells in the ultimobranchial glands showed immunoreactivity for TuJ1 invariable degrees, weak to intense. At 12 days of incubation, a vast majority of C cells were intensely immunoreactive for TuJ1, and further TuJ1-immunoreactive nerve fibers were distributed in the ultimobranchial glands. At 14 and 16 days of incubation, intense immunoreactivity for TuJ1 was sustained in the C cells. Electron microscopic analyses revealed that TuJ1 immunoreactivity was diffusely distributed throughout the cytoplasm and also localized on the secretory granules of C cells at these stages. TuJ1 immunoreactivity in the C cells started to decrease at late stages of embryonic development. At the hatching period, dense distributions of TuJ1-immunoreactive nerve fibers were observed in the ultimobranchial glands, whereas TuJ1 immunoreactivity of the C cells became very weak. In 10-day-old chickens, TuJ1 immunoreactivity was restricted to the nerve fibers.
Kameda
Y
Y
Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
Kameya
T
T
Frankfurter
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D002116:Calcitonin / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002642:Chick Embryo; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D009474:Neurons / Q000737:chemistry*; D014404:Tubulin / Q000032:analysis*; D014460:Ultimobranchial Body / Q000737:chemistry* / Q000196:embryology
D000911:Antibodies, Monoclonal; D014404:Tubulin; D002116:Calcitonin