Correction of absorption spectra of a pH-sensitive dye for intracellular pH estimation in smooth muscle.
Methods of correction for intracellular pH (pHi) estimation from the absorbance of the pH-sensitive dye, 4',5'-dimethyl-5-(and -6-) carboxy-fluorescein (Me2CF), were described. In the guinea-pig vena cava, a reasonable estimation of pHi changes was obtained when the distortion of the spectrum was corrected using the tissue absorbance at wavelengths longer than 570 nm.
Matsumoto
T
T
Department of Physiology, School of Medicine, Nagoya University, Japan.
Tomita
T
T
D000818:Animals; D004396:Coloring Agents / Q000737:chemistry*; D005260:Female; D005452:Fluoresceins / Q000737:chemistry*; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014682:Vena Cava, Inferior; D014683:Vena Cava, Superior
D004396:Coloring Agents; D005452:Fluoresceins; C047665:4',5'-dimethyl-5-carboxyfluorescein