p53 protein over-expression and p53 gene abnormalities in HIV-1-related non-Hodgkin's lymphomas.
Alteration of the p53 tumor-suppressor gene was studied in non-Hodgkin's lymphomas (NHLs) from HIV-1-infected patients. p53 protein was over-expressed in 10 out of the 45 (22%) cases analyzed, mainly clustering in the small-non-cleaved-cell (SNC) (5/19) and Ki-1+ anaplastic large-cell (ALC) (3/8) sub-types, according to previous findings on HIV-1-unrelated NHLs. p53-positive small-non-cleaved-cell lymphomas presented a diffuse or clustered pattern of p53-positive neoplastic cells consequent upon p53-gene mutations. In contrast, in Ki-1+ ALC lymphomas p53 immunohistochemical reactivity was limited to scattered tumor cells, and no p53-gene alterations could be detected. These results suggest that p53-gene alterations play a role in the lymphomagenetic process of a fraction of HIV-1-related SNC NHLs, however with a frequency no different from that observed in HIV-1-unrelated NHLs of the same sub-type. In HIV-1-related Ki-1+ ALC lymphomas, mechanisms different from gene alterations might be implicated in over-expression of p53 protein.
De Re
V
V
Division of Experimental Oncology 1, Centro di Riferimento Oncologico, Aviano, Italy.
Carbone
A
A
De Vita
S
S
Gloghini
A
A
Maestro
R
R
Gasparotto
D
D
Vukosavljevic
T
T
Boiocchi
M
M
D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D015870:Gene Expression; D016158:Genes, p53; D015658:HIV Infections / Q000150:complications*; D015497:HIV-1; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000150:complications / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D012367:RNA, Viral; D016159:Tumor Suppressor Protein p53