Gangliosides of Echinococcus multilocularis metacestodes.
Gangliosides, glycosphingolipids with sialic acid, were found in metacestodes of Echinococcus multilocularis in low quantities. All gangliosides were resolved after preparative high-performance thin layer chromatography into four fractions. Cholera toxin was specifically bound to the major ganglioside, allowing the identification of it as a GM1. Precise structure of the four fractions was determined by sequential degradation by exoglycosidases, gas chromatography, electron impact mass spectrometry and liquid secondary ion-mass spectrometry. Beside GM1, the other fractions were GM3, GD1a and, at a lesser percentage, GM2, all belonging to the same a-ganglio-series. The ceramide part of these parasite gangliosides contained sphingosine associated to unsaturated n24, saturated n24 and n16 fatty acids.
Persat
F
F
Département de Parasitologie et Pathologie Exotique, Université Claude Bernard, Lyon, France.
Bouhours
J F
JF
Petavy
A F
AF
Mojon
M
M
D000818:Animals; D002240:Carbohydrate Sequence; D004446:Echinococcus / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D005732:Gangliosides / Q000032:analysis / Q000302:isolation & purification*; D006026:Glycoside Hydrolases; D008969:Molecular Sequence Data; D009439:Neuraminidase; D013110:Sphingosine / Q000032:analysis
D005227:Fatty Acids; D005732:Gangliosides; D006026:Glycoside Hydrolases; C051111:ceramide glycanase; D009439:Neuraminidase; D013110:Sphingosine