title: Enhanced response to daunomycin of normal, tumor and metastatic cell lines via drug photoactivation.
abstract: We have compared the cytotoxicity of daunomycin in vitro to highly differentiated normal epithelial cells (Fisher rat thyroid cells, FRTL-5) and to two neoplastic cell lines, a thyroid carcinoma (TK-6) and its lung metastasis (MPTK-6). Whereas the cell lines are equally sensitive to the drug in the dark, if irradiated during incubation with daunomycin (86 J/cm2 at 488 nm), they become more and differently sensitive. Namely, the drug doses producing 50% mortality decrease by factors of about 22, 28 and 16 for FRTL-5, TK-6 and MPTK-6 cell lines, respectively. This result correlates with differences in drug uptake and resistance observed in the normal and neoplastic cell lines.
authors.affiliation: C.N.R., Centro Endocrinologia ed Oncologia Sperimentale (C.E.O.S.), Napoli, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Andreoni
authors.forename: A
authors.initials: A
authors.lastname: Colasanti
authors.forename: A
authors.initials: A
authors.lastname: Kisslinger
authors.forename: M
authors.initials: M
authors.lastname: Mastrocinque
authors.forename: G
authors.initials: G
authors.lastname: Portella
authors.forename: P
authors.initials: P
authors.lastname: Riccio
authors.forename: G
authors.initials: G
authors.lastname: Roberti
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D003624:Darkness; D003630:Daunorubicin / Q000378:metabolism / Q000528:radiation effects* / Q000633:toxicity*; D008027:Light; D008175:Lung Neoplasms / Q000556:secondary; D051381:Rats; D011916:Rats, Inbred F344; D013961:Thyroid Gland; D013964:Thyroid Neoplasms; D014407:Tumor Cells, Cultured
chemical_list: D003630:Daunorubicin