title: Characterization of a Rana catesbeiana lectin-resistant mutant of leukemia P388 cells.
abstract: Sialic acid-binding lectin (SBL-C) from Rana catesbeiana eggs inhibits the growth of tumor cells such as P388 and L1210 leukemia cells (K. Nitta et al., Cancer Res., 54: 920-927, 1994). Here we report the establishment of an SBL-resistant P388 variant cell line, RC-150. Both P388 and RC-150 cells were agglutinated by SBL-C; however, growth of RC-150 cells was unaffected by SBL-C. Cytoplasmic free Ca2+ concentration and transglutaminase activity of RC-150 cells were 0.5 (110 nM) and 3 times (0.62 nmol/mg/min) as high as those of P388 cells, respectively. Microvilli and microplicae were observed on the surface of P388 cells by scanning electron microscopy but were rarely seen on RC-150 cells. Dansylcadaverine-labeled SBL-C bound to both P388 and RC-150 cells. Binding of SBL-C to these tumor cells appears to be mediated by two species of wheat germ agglutinin-stained cell membrane sialoglycoproteins. Labeled SBL-C entered P388 but not RC-150 cells, suggesting that internalized SBL-C acts as an inhibitor of cell proliferation.
authors.affiliation: Cancer Research Institute, Tohoku College of Pharmaceutical Sciences, Sendai, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nitta
authors.forename: K
authors.initials: K
authors.lastname: Ozaki
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: S
authors.initials: S
authors.lastname: Furusawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkubo
authors.forename: H
authors.initials: H
authors.lastname: Takimoto
authors.forename: R
authors.initials: R
authors.lastname: Murata
authors.forename: M
authors.initials: M
authors.lastname: Hosono
authors.forename: N
authors.initials: N
authors.lastname: Hikichi
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D000371:Agglutination; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004351:Drug Resistance; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D007941:Leukemia P388 / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D011892:Rana catesbeiana; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012794:Sialic Acids / Q000032:analysis
chemical_list: D037102:Lectins; D008562:Membrane Glycoproteins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012794:Sialic Acids; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D002118:Calcium