Gas chromatography/mass spectrometric analysis of urinary nucleosides in cancer patients; potential of modified nucleosides as tumour markers.
Analysis of urine from cancer patients by capillary gas chromatography/mass spectrometry positively identified 14 urinary nucleosides including several modified nucleosides. Levels of the modified nucleosides 1-methyl-adenosine, 2-methylguanosine, N2,N2-dimethylguanosine and 1-methylinosine as well as the total nucleoside level were elevated in the urine when a malignant tumour was present; the levels of N2,N2-dimethylguanosine were found to correlate with the stage of the cancer.
Langridge
J I
JI
Biochemistry Research Group (SoBS), University College of Swansea, UK.
McClure
T D
TD
el-Shakawi
S
S
Fielding
A
A
Schram
K H
KH
Newton
R P
RP
D000241:Adenosine / Q000031:analogs & derivatives / Q000652:urine; D014408:Biomarkers, Tumor; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006151:Guanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D007288:Inosine / Q000031:analogs & derivatives* / Q000652:urine; D008297:Male; D009369:Neoplasms / Q000652:urine*; D009705:Nucleosides / Q000652:urine*
D014408:Biomarkers, Tumor; D009705:Nucleosides; D006151:Guanosine; C002230:1-methyladenosine; C008940:N(2),N(2)-dimethylguanosine; C012433:1-methylinosine; D007288:Inosine; D000241:Adenosine