[The determination of T-lymphocyte antigenic markers in the serum of patients with atopic dermatitis].
The euglobulin fraction of serum of the patients with atopic dermatitis was studied using monoclonal antibodies OKT3, OKT8 and OKT10 in assay system including secondary 125J labeled anti-mouse IgG antibodies. It was shown that CD3, CD8 and CD38 T-cell marker antigens shed from the T-cell surface could be found in euglobulin fraction of serum patients. The patients with atopic dermatitis successfully treated with immunomodulator vilosen were selected and their serums obtained before and after treatment were investigated. The treatment resulted in the increase of CD8, CD3 determinants and a decrease of antigens determined by OKT10 antibodies (CD38). It is supposed that an increase of CD3 and CD8 determinants is caused by stimulation of T-lymphocyte proliferation and suppressor cell function by vilosen. Since CD38 marker is predominantly on the surface of immature and activated T-cells, which do not occur in circulating blood, it can be supposed that decrease of CD38 in the euglobulin fraction of serum reflects a shedding of these determinants from T-cells of thymus and peripheral lymphoid organs.
Bezvershenko
I A
IA
Boĭko
M G
MG
Kaliuzhnaia
L D
LD
D000276:Adjuvants, Immunologic / Q000494:pharmacology / Q000627:therapeutic use; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000097:blood* / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D017252:CD3 Complex / Q000097:blood / Q000187:drug effects; D016827:CD8 Antigens / Q000097:blood / Q000187:drug effects; D002455:Cell Division; D003876:Dermatitis, Atopic / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013949:Thymus Extracts / Q000494:pharmacology / Q000627:therapeutic use
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015415:Biomarkers; D017252:CD3 Complex; D016827:CD8 Antigens; D007074:Immunoglobulin G; D013949:Thymus Extracts; C059606:iilozen
Vyznachennia antigennykh determinant, prytamannykh T-limfotsytam, u syrovattsi krovi khvorykh na atopichnyĭ dermatyt.