[Features of connecting short oligonucleotides with phage T4 DNA ligase].
To understand the influence of different factors on the efficiency of T4 DNA ligase-catalyzed connection of oligonucleotides, a comparative study of polycondensation of AT-containing concatemer octanucleotides (some of them potentially can form "parallel" duplexes) has been undertaken. It has been shown that the size of substrate duplex is the most significant factor, while the sequence of oligonucleotides influences the enzymatic reaction indirectly, by determining the thermal stability of double-stranded complexes. In practice, none of the octanucleotides under study does form "parallel" duplexes.
Shilov
I A
IA
Koroleva
O N
ON
Drutsa
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VL
D001345:Autoradiography; D001483:Base Sequence; D011088:DNA Ligases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism*; D013604:T-Phages / Q000378:metabolism*
D009841:Oligonucleotides; D011088:DNA Ligases
Osobennosti soedineniia korotkikh oligonukleotidov DNK-ligazoĭ faga T4.