[The karyotypic characteristics of a skin fibroblast line from the Indian muntjac cultured with different sera].
The karyotypic structure of the Indian muntjak skin fibroblast cell line, with 7 chromosomes in modal class, was compared in the cells cultures, with 10 and 3% of fetal calf serum of two different lots. In addition, the effect of the same serum lot on chromosome variability in the two different Indian muntjac skin fibroblast sublines differing in the modal class stability was investigated. In both the cases, the serum affects the cell distribution for the chromosome number. The analysis of correlations between the main and other structure variants of the karyotype (MSVK and SVK) showed that the karyotypic variants are selected for the balanced genotype rather than for particular chromosomes. The changed cultural conditions resulted primary in the selection of the preexisting SVK to convert into MSVK. The lowering in the serum content to 3% induced the increase in chromosome aberrations. The frequency of chromosome aberrations depends on both the serum lot and the chromosomal composition of sublines. Chromosomal breaks and dicentrics are the predominant types of chromosomal disturbances. Dicentrics are produced mainly by chromosomes 1 and 2. The data obtained may suggest that sera could affect karyological characteristics of cultured cells. This indicates that the cytogenetical monitoring is necessary during a long-term cultivation of cell lines.
Polianskaia
G G
GG
Sizova
L S
LS
Nikolaenko
N S
NS
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000648:ultrastructure; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure*; D003470:Culture Media; D003670:Deer / Q000235:genetics*; D005347:Fibroblasts / Q000648:ultrastructure; D014644:Genetic Variation; D007621:Karyotyping; D008677:Metaphase; D012867:Skin / Q000648:ultrastructure*
D003470:Culture Media
Kariotipicheskie kharakteristiki linii fibroblastov kozhi indiĭskogo muntzhaka pri kul'tivirovanii s raznymi syvorotkami.