title: Purification and characterization of collagens from rat fibrosarcoma induced by 3-methylcholanthrene.
abstract: The distribution of various collagen types was studied in rat fibrosarcoma. Collagens extracted from fibrosarcoma tissue were characterized by the criteria of solubility in NaCl, SDS-PAGE, ion exchange chromatography, CNBr peptide mapping and amino acid analysis. Fibrosarcoma was found to produce excess amount of type V, type I trimer and type III collagens; comparatively, type I collagen and total collagen content were noticed to decrease in fibrosarcoma. We observe that the increase in type V collagen content in fibrosarcoma might be due to the enhanced transcription of type V collagen gene. Increased type I trimer collagen in fibrosarcoma might be attributed to the differential expression of alpha 1(I) and alpha 2(I) gene and might also be due to the expression of a different gene for type I trimer collagen.
authors.affiliation: Department of Biochemistry, School of Chemical Sciences, University of Georgia, Athens 30602.
authors.forename: R
authors.initials: R
authors.lastname: Asokan
authors.forename: R
authors.initials: R
authors.lastname: Puvanakrishnan
authors.forename: L V
authors.initials: LV
authors.lastname: Ravichandran
authors.forename: V
authors.initials: V
authors.lastname: Kokila
authors.forename: G K
authors.initials: GK
authors.lastname: Reddy
authors.forename: S C
authors.initials: SC
authors.lastname: Dhar
mesh_terms: D000818:Animals; D003094:Collagen / Q000302:isolation & purification*; D005354:Fibrosarcoma / Q000139:chemically induced / Q000737:chemistry*; D008297:Male; D008748:Methylcholanthrene; D051381:Rats; D017208:Rats, Wistar; D012983:Soft Tissue Neoplasms / Q000139:chemically induced / Q000737:chemistry*; D012995:Solubility
chemical_list: D008748:Methylcholanthrene; D003094:Collagen