title: A rapid and simple method for reversing the inhibitory effect of heparin on PCR for HLA class II typing.
authors.affiliation: Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico, Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Poli
authors.forename: R
authors.initials: R
authors.lastname: Cattaneo
authors.forename: L
authors.initials: L
authors.lastname: Crespiatico
authors.forename: A
authors.initials: A
authors.lastname: Nocco
authors.forename: G
authors.initials: G
authors.lastname: Sirchia
mesh_terms: D002614:Chelating Agents; D004247:DNA / Q000097:blood / Q000235:genetics; D005069:Evaluation Studies as Topic; D005784:Gene Amplification; D005802:Genes, MHC Class II; D006493:Heparin / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D019384:Nucleic Acid Synthesis Inhibitors; D016133:Polymerase Chain Reaction / Q000379:methods*; D012117:Resins, Synthetic; D019914:Taq Polymerase
chemical_list: D002614:Chelating Agents; D019384:Nucleic Acid Synthesis Inhibitors; D012117:Resins, Synthetic; C024997:Chelex 100; D006493:Heparin; D004247:DNA; D019914:Taq Polymerase