title: Influence of hematocrit on tissue O2 extraction capabilities during acute hemorrhage.
abstract: This study was performed to test the hypothesis that tissue O2 extraction capabilities during hemorrhage may be greater when hematocrit (Hct) is initially reduced. Twenty-four anesthetized and splenectomized dogs were randomly assigned in three groups of eight dogs each: group 1 (Hct 40), 40-45% Hct; group 2 (Hct 30), 30-35% Hct; and group 3 (Hct 20), 20-25% Hct. In each animal, the desired Hct was obtained by normovolemic hemodilution using hydroxyethyl starch 450/0.7 and maintained throughout the experiment. O2 delivery (DO2) was progressively reduced by hemorrhage. At each step, DO2 and O2 consumption (VO2) were measured separately. Critical DO2 obtained from a plot of VO2 vs. DO2 was lower in the Hct 30 and Hct 20 groups than in the Hct 40 group [(in ml.min-1.kg-1) Hct 30, 7.9 +/- 2.2; Hct 20, 7.8 +/- 1.0; Hct 40, 10.4 +/- 1.1; P < 0.05]. Critical DO2 obtained from blood lactate levels was also significantly lower in the Hct 30 and Hct20 groups than in the Hct 40 group. Critical O2 extraction ratio was also greater in the Hct 30 and Hct 20 groups than in the Hct 40 group (Hct 30, 73.0 +/- 13.9%; Hct 20, 70.1 +/- 9.6%; Hct 40, 57.1 +/- 11.5%; P < 0.05). In the conditions of our study, moderate hemodilution was associated with an improvement of the O2 extraction capabilities of the body, probably related to the reduction in blood viscosity.
authors.affiliation: Department of Anesthesiology, Erasme University Hospital, Free University of Brussels, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Van der Linden
authors.forename: E
authors.initials: E
authors.lastname: Gilbart
authors.forename: P
authors.initials: P
authors.lastname: Paques
authors.forename: C
authors.initials: C
authors.lastname: Simon
authors.forename: J L
authors.initials: JL
authors.lastname: Vincent
mesh_terms: D000208:Acute Disease; D000818:Animals; D001682:Biological Availability; D004285:Dogs; D006400:Hematocrit; D006438:Hemodilution; D006470:Hemorrhage / Q000378:metabolism*; D010100:Oxygen / Q000493:pharmacokinetics; D010101:Oxygen Consumption; D013156:Splenectomy
chemical_list: D010100:Oxygen