Simple phagemid-based system for generating allele replacements in Escherichia coli.
We describe a phagemid-based system for rapidly generating chromosomal allele replacements in two steps. The system utilizes simple vector-determined selection procedures for each step and requires only that the target strain be male and sup+ (nonsuppressing for amber mutations).
Slater
S
S
Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4960.
Maurer
R
R
D002876:Chromosomes, Bacterial; D003090:Coliphages / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic