Iodine induced lymphocytic thyroiditis in the BB/W rat: early and late immune phenomena.
The effect of iodine excess on thyroid function and on the immunological sequence of events leading to lymphocytic thyroiditis (LT) was studied in the NB subline of BB/W rats to determine the mechanisms by which the level of iodine intake influences the development of LT in this animal model. Iodine supplemented water (500 micrograms/l, Group 1 or 500 mg/l, Group 2) or non-iodine supplemented tap water (Group 3) was given to breeding pairs and their offspring ad libitum. A Wistar rat group, also given tap water (Group 4) served as controls. To determine the immunological sequence of events, the phenotypic nature of the infiltrating thyroid lymphocytes was examined by specific immunoperoxidase staining in BB/W and Wistar rats at 6, 9, 12, and 15 weeks. Antigen-presenting cells and class II (Ia) antigen expression on thyrocytes were also examined. The first immunological event apparent in the iodine-treated BB/W rats was a sharp increase in the number of Ia positive dendritic cells at 9 weeks compared with control BB/W and Wistar rats. In the iodine excess groups dendritic cells were associated with scattered areas of lymphocytic infiltration, comprising predominantly T helper cells (W3/25). T suppressor cells (OX 8) and IL-2 receptor positive activated T-cells (OX 39) were both present in small numbers. B-cells (OX 12) were absent. In addition, thyrocytes did not exhibit Ia antigen expression. By contrast, lymphocytic infiltration was not found at 9 weeks in control BB/W rats.(ABSTRACT TRUNCATED AT 250 WORDS)
Li
M
M
Department of Clinical Endocrinology, Westmead Hospital, NSW, Australia.
Eastman
C J
CJ
Boyages
S C
SC
D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007150:Immunohistochemistry; D007455:Iodine / Q000633:toxicity*; D008213:Lymphocyte Activation / Q000187:drug effects; D051381:Rats; D011913:Rats, Inbred BB; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000187:drug effects; D013961:Thyroid Gland / Q000473:pathology; D013967:Thyroiditis, Autoimmune / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology
D000949:Histocompatibility Antigens Class II; D007455:Iodine