Improved alcohol-fixed smears of fine-needle aspirates for cytology of breast and other palpable lesions.
The application of fine-needle aspiration cytology for the diagnosis of solid lesions is well-established. Its sensitivity as a diagnostic tool for both benign and malignant lesions can vary significantly, however, and depends on the quality of the smears submitted. In particular, air-drying artifact can render wet alcohol-fixed smears unsatisfactory for diagnosis. This study examines the basic cause of air-drying artifacts, which is directly related to the moisture of the aspirate that varies among tissues and specimens from any tumor type. A simple modification to the standard smearing technique incorporating precoated slides that introduces moisture into the aspirate is recommended. The technique is applicable to aspirates from all sites without compromising accepted diagnostic cytologic criteria, and offers excellent cytologic clarity with routine stains as well as being flexible enough for other investigative staining methods.
Nathan
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NA
Cytology Laboratory, Anatomical Pathology Division, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia.
D016477:Artifacts; D001707:Biopsy, Needle / Q000379:methods*; D001940:Breast / Q000166:cytology / Q000473:pathology; D001941:Breast Diseases / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology*; D000431:Ethanol; D005260:Female; D006258:Head and Neck Neoplasms / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D009369:Neoplasms / Q000473:pathology*; D010307:Parotid Neoplasms / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology; D012189:Retrospective Studies; D013964:Thyroid Neoplasms / Q000473:pathology
D000431:Ethanol