title: Effect of monoclonal anti-ANP antibodies on the acute functional adaptation to unilateral nephrectomy.
abstract: The role of endogenous atrial natriuretic peptide (ANP) in the immediate response of sodium excretion to unilateral nephrectomy (UNX) was investigated in anesthetized euvolemic rats through measurement of UNX-induced change in plasma ANP concentration and the response of the remaining kidney to UNX following administration of monoclonal anti-ANP antibodies. The circulating ANP levels almost tripled (from 23 +/- 4 to 66 +/- 13 fmol/ml, P < 0.01) within two minutes after UNX, whereas no change resulted from sham intervention. In the control group receiving vehicle injection, UNX resulted in a twofold increase in urinary sodium excretion (from 1.39 +/- 0.25 to 2.88 +/- 0.28 mumol/min, P < 0.01) related to a decrease in the fractional reabsorption of sodium at both proximal and distal sites (estimated from fractional excretion of lithium). Urinary excretion of cyclic guanosine 3'-5'-monophosphate (cGMP) increased as well, but glomerular filtration rate did not change. In addition, UNX was associated with a short-lived (< 20 min) rise in systemic arterial pressure and a transient fall in right atrial pressure. Administration of monoclonal anti-ANP antibodies totally prevented the UNX-associated natriuresis by blunting both proximal and distal tubular reabsorption of sodium, and suppressed the rise in urinary cGMP excretion following UNX. The duration of the post-UNX increase in arterial pressure was longer when compared to values observed in controls. These observations indicate that ANP release is stimulated after uninephrectomy.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, Hôpital Lapeyronie, Montpellier, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Valentin
authors.forename: J
authors.initials: J
authors.lastname: Ribstein
authors.forename: D
authors.initials: D
authors.lastname: Neuser
authors.forename: J
authors.initials: J
authors.lastname: Nüssberger
authors.forename: A
authors.initials: A
authors.lastname: Mimran
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000502:physiology*; D001794:Blood Pressure; D008297:Male; D009392:Nephrectomy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000911:Antibodies, Monoclonal; D009320:Atrial Natriuretic Factor