title: Determination of diltiazem hydrochloride in human serum by high-performance liquid chromatography.
abstract: A simple and sensitive reversed-phase high-performance liquid chromatographic method for the determination of diltiazem in human serum has been developed. The method involves a one-step deproteinization of serum for sample clean-up using acetonitrile. A LiChrosorb RP-8 column (30 cm x 4.1 mm I.D.) was eluted isocratically with acetonitrile-0.01 M dibasic sodium phosphate (40:60, v/v) containing 0.01% triethanolamine. Diltiazem was monitored at 237 nm and 0.1 a.u.f.s. The completion time for assay was less than 15 min, and the lower limit of quantitation was 10 ng/ml for a 100-microliters injection volume. Using this method, the pharmacokinetic parameters were calculated from a serum concentration versus time profile of diltiazem.
authors.affiliation: Lupin Laboratories Limited, MIDC, Chikalthana, Aurangabad, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Chaudhary
authors.forename: S S
authors.initials: SS
authors.lastname: Gangwal
authors.forename: M K
authors.initials: MK
authors.lastname: Avachat
authors.forename: Y N
authors.initials: YN
authors.lastname: Shah
authors.forename: K C
authors.initials: KC
authors.lastname: Jindal
mesh_terms: D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004110:Diltiazem / Q000097:blood* / Q000493:pharmacokinetics; D004983:Ethanolamines; D006801:Humans; D007700:Kinetics; D012972:Sodium Hydroxide
chemical_list: D000097:Acetonitriles; D004983:Ethanolamines; D012972:Sodium Hydroxide; C009546:triethanolamine; D004110:Diltiazem; C032159:acetonitrile