Scanning electrochemical microscopy. 23. Reaction localization of artificially patterned and tissue-bound enzymes.
The scanning electrochemical microscope (SECM), operating in the feedback mode, was used to image localized surface reactions of redox enzymes at the micrometer level. Surfaces imaged with the SECM included glucose oxidase immobilized within 8-microns-diameter pores of a filtration membrane and individual whole mitochondria with active NADH cytochrome reductase enzymes in their outer membranes. Factors influencing enzyme image resolution and specificity are discussed.
Pierce
D T
DT
Department of Chemistry and Biochemistry, University of Texas at Austin 78712.
Bard
A J
AJ
D000818:Animals; D004563:Electrochemistry / Q000379:methods; D005949:Glucose Oxidase / Q000032:analysis*; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008930:Mitochondria, Liver / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000032:analysis*; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013379:Substrate Specificity
D005949:Glucose Oxidase; D009245:NADH Dehydrogenase