title: Interleukin-5 (IL-5) and IL-6 define two molecularly distinct pathways of B-cell differentiation.
abstract: Interleukin-5 (IL-5) and IL-6 have both been reported to act as B-cell differentiation factors by stimulating activated B cells to secrete antibody. However, it has not been possible to directly compare the effects of these two lymphokines because of the lack of a suitable B-cell line capable of responding to both. We have identified a clonal, inducible B-cell lymphoma, CH12, that has this property. Both IL-5 and IL-6 can independently stimulate increases in steady-state levels of immunoglobulin and J-chain mRNA and proteins, and they both induce the differentiation of CH12 into high-rate antibody-secreting cells. Nevertheless, there are significant differences in the activities of these two lymphokines. First, while IL-6 acts only as a differentiation factor, IL-5 also augments the proliferation of CH12 cells. Second, the differentiation stimulated by IL-5 but not by IL-6 is partially inhibited by IL-4. Inhibition of IL-5-induced differentiation was not at the level of IL-5 receptor expression, since IL-4 did not inhibit IL-5-induced proliferation. Third, IL-5 but not IL-6 stimulated increased mouse mammary tumor proviral gene expression in CH12 cells. These results demonstrate that while both IL-5 and IL-6 may act as differentiation factors for B cells, they induce differentiation by using at least partially distinct molecular pathways. Our results also establish that B cells characteristic of a single stage of development can independently respond to IL-4, IL-5, and IL-6.
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: T D
authors.initials: TD
authors.lastname: Randall
authors.forename: F E
authors.initials: FE
authors.lastname: Lund
authors.forename: J W
authors.initials: JW
authors.lastname: Brewer
authors.forename: C
authors.initials: C
authors.lastname: Aldridge
authors.forename: R
authors.initials: R
authors.lastname: Wall
authors.forename: R B
authors.initials: RB
authors.lastname: Corley
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D005803:Genes, Immunoglobulin; D007144:Immunoglobulin J-Chains / Q000378:metabolism; D015848:Interleukin-5 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D014407:Tumor Cells, Cultured
chemical_list: D007144:Immunoglobulin J-Chains; D015848:Interleukin-5; D015850:Interleukin-6