title: Influence of constant fluoride levels in solution on root hard tissue de- and remineralization measured by 125I absorptiometry.
abstract: To study the effect of fluoride on de- and remineralization of root hard tissue, an automatic pH-cycling caries model, simulating Stephan curves, was used for 21 days. From each of 13 unexposed human roots, four cementum/dentin blocks were prepared. Four experiments were carried out: one block from each tooth as subjected to pH cycling without and with fluoride at the concentrations of 0.02 ppm (1.0 mumol/l), 0.20 ppm (10.5 mumol/l) and 2.00 ppm (105.3 mumol/l) in the de- and remineralizing solutions, respectively. Mineral change in the specimens was monitored by 125I absorptiometry. When no fluoride was added to the solutions the change in transmission (delta T) increased continuously over 21 days, indicating loss of mineral. In the 0.02-ppm F experiment, there was a marked decrease in delta T, but almost no change in the 0.20-ppm F experiment. pH cycling with 2.00 ppm F in the solutions resulted in a gain of mineral in or most likely on the surface of the cementum/dentin blocks, indicated by a continuously decreasing delta T. The fluoride level in the solution significantly influenced the change in the mineral content of the specimens. Microradiographs of the sectioned blocks showed radiodense surface zones, varying degrees of subsurface demineralization and signs of remineralization or mineral deposition.
authors.affiliation: Department of Cariology, School of Dentistry, Karolinska Institute, Huddinge, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Almqvist
authors.forename: F
authors.initials: F
authors.lastname: Lagerlöf
mesh_terms: D015502:Absorptiometry, Photon; D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D002122:Calcium Chloride / Q000494:pharmacology; D003739:Dental Cementum / Q000737:chemistry / Q000187:drug effects; D003804:Dentin / Q000737:chemistry / Q000187:drug effects; D005459:Fluorides / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes; D008852:Microradiography; D009584:Nitrogen / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D017001:Tooth Demineralization / Q000378:metabolism / Q000503:physiopathology*; D014089:Tooth Remineralization; D014092:Tooth Root / Q000737:chemistry / Q000187:drug effects*
chemical_list: D000085:Acetates; D007457:Iodine Radioisotopes; D010710:Phosphates; C003320:sodium nitride; D011189:Potassium Chloride; D002122:Calcium Chloride; D009584:Nitrogen; D019342:Acetic Acid; D005459:Fluorides; D006851:Hydrochloric Acid; C018279:sodium phosphate