"PCR karyotype" of monochromosomal somatic cell hybrids.
Following fusion of human diploid fibroblast-derived microcells with mouse A9 cells, we isolated seven monochromosomal hybrids containing a single human chromosome 2, 3, 5, 12, 15, 20, or 22. Cytogenetic analysis as well as PCR karyotyping (chromosome-specific banding pattern generated by Alu-PCR) was performed on all the hybrids. We here present, for the first time, the specific PCR karyotypes of human chromosomes 2 and 20.
Ning
Y
Y
Roy M. and Phyllis Gough Huffington Center on Aging, Baylor College of Medicine, Houston, Texas 77030.
Lovell
M
M
Cooley
L D
LD
Pereira-Smith
O M
OM
D000818:Animals; D002871:Chromosome Banding; D002881:Chromosomes, Human, Pair 12; D002889:Chromosomes, Human, Pair 2; D002890:Chromosomes, Human, Pair 20; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping / Q000379:methods; D051379:Mice; D016133:Polymerase Chain Reaction