Glutathione metabolism in skeletal muscle derived cells of the L6 line.
Skeletal muscle derived L6 myoblasts possess a considerably high resting total glutathione (TGSH) pool. Exposure to L-buthionine-[S,R]-sulphoximine resulted in a 90% depletion of the intracellular TGSH pool. All the key enzymes of glutathione metabolism, especially glutathione S-transferase, were observed to be considerably active in the undifferentiated cells. Se-dependent glutathione peroxidase activity appeared to account for most of the total GSH peroxidase activity of the cells. A significant contribution of gamma-glutamyl transpeptidase-independent (5 mM acivicin insensitive) mechanism to the extracellular GSH uptake capacity of the muscle cells was evident. Efflux of oxidized glutathione from the cells exposed to t-butyl hydroperoxide was rapid and appeared to be energy linked.
Sen
C K
CK
Department of Physiology, University of Kuopio, Finland.
Rahkila
P
P
Hänninen
O
O
D028341:Activins; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D019328:Buthionine Sulfoximine; D002460:Cell Line; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D005978:Glutathione / Q000032:analysis / Q000378:metabolism*; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism; D007265:Inhibins / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D009132:Muscles / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D020122:tert-Butylhydroperoxide
D000963:Antimetabolites; D010545:Peroxides; D028341:Activins; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D007265:Inhibins; D020122:tert-Butylhydroperoxide; D005982:Glutathione Transferase; D005978:Glutathione