Antigen dependent release of interleukin 5 in vitro from spleen cells of mice infected with Angiostrongylus cantonensis.
The kinetics of eosinophil growth and/or survival stimulating factors (Eo-stimulating factors) released by spleen cells from A. cantonensis-infected mice were assessed by in vitro marrow cultures. When spleen cells from C57BL/6 mice 8 to 20 days p.i. were cultured with young adult A. cantonensis worm antigen or Con A, almost equal amounts of Eo-stimulating factors were detected in the conditioned media obtained from both stimulations. No Eo-stimulating factor activity was detected in cultures from spleen cells without stimulation or from normal spleen cells with stimulation. Production of Eo-stimulating factors was inhibited by the pretreatment of the spleen cells with anti-Thy1.2 or anti-L3T4 antibodies plus complement but not with anti-Lyt2.2 antibody. In the presence of anti-mouse IL-5 monoclonal antibody, the activity of Eo-stimulating factors was inhibited by up to 99%. IL-5, therefore, appears to play a principal role in induction of eosinophilia in mice infected with A. cantonensis.
Sugaya
H
H
Department of Parasitology, Akita University School of Medicine, Japan.
Abe
T
T
Yoshimura
K
K
Sasaki
O
O
D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001854:Bone Marrow Cells; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D004804:Eosinophils / Q000166:cytology; D005260:Female; D015848:Interleukin-5 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D013154:Spleen / Q000276:immunology*; D017206:Strongylida Infections / Q000276:immunology*
D000947:Antigens, Helminth; D015848:Interleukin-5