[Detection of mRNA in electronic microscopy: in situ hybridization of a biotin-labeled oligonucleotide prior to embedding].
A technique for mRNA detection by ultrastructural in situ hybridization is reported. Vibratome sections are hybridized with a biotinylated oligonucleotide probe which is detected using an enhanced avidin-peroxidase technique. Sections are then osmicated and embedded in epoxy resin. This rapid, sensitive technique achieved ultrastructural detection of vasopressin mRNA in magnocellular neurons of rat hypothalamus.
Trembleau
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Laboratoire de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Université P. et M. Curie, Paris, France.
D000818:Animals; D001710:Biotin; D007031:Hypothalamus / Q000502:physiology; D017403:In Situ Hybridization / Q000379:methods*; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D014667:Vasopressins / Q000235:genetics*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014667:Vasopressins; D001710:Biotin
Détection d'ARNm en microscopie électronique: hybridation in situ d'un oligonucléotide biotinylé en pré-inclusion.