HPLC determination of D-glucaric acid in human urine.
An isocratic HPLC method has been developed for the direct measurement of D-glucaric acid in human urine. Pretreatment of urine with a boronic acid gel removed many interfering substances, including L-ascorbic acid and D-glucuronic acid. This method has a detection limit of 10 microM D-glucaric acid (approximately 7 mumoles/g creatinine). The run-to-run precisions were 9.1% and 7.7% at urinary D-glucaric acid concentrations of 41 and 219 mumoles/g creatinine, respectively. Urinary D-glucaric acid concentrations in normal adults were found to cover a range of 15 to 89 mumoles/g creatinine (mean = 47 mumoles/g creatinine). The sensitivity of this method in detecting abnormal elevations in D-glucaric acid was demonstrated through its ability to measure changes in urinary concentrations with time after ingestion of D-glucuronolactone.
Poon
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Bureau of Chemical Hazards, Health and Welfare Canada, Ottawa.
Villeneuve
D C
DC
Chu
I
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Kinach
R
R
D000328:Adult; D001205:Ascorbic Acid / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D003404:Creatinine / Q000097:blood; D005260:Female; D005937:Glucaric Acid / Q000652:urine*; D005965:Glucuronates / Q000652:urine; D020723:Glucuronic Acid; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D012727:Sex Characteristics
D005965:Glucuronates; D007202:Indicators and Reagents; D020723:Glucuronic Acid; D003404:Creatinine; D001205:Ascorbic Acid; D005937:Glucaric Acid