Glucose transporter (GLUT1) expression by canine brain microvessel endothelial cells in culture: an immunocytochemical study.
We have found that immunocytochemical techniques can be used to monitor the qualitative expression of GLUT1 in cultured cells. Primary cultures of BMEC showed significant GLUT1 staining, and primary cultures of endothelial cells from a non-barrier source (aorta) were negative. BMEC express significant levels of GLUT1 in young primary cultures, thus affording an opportunity for short-term regulation studies of GLUT1 activity and expression. Immunocytochemistry may be an efficient method for screening potential GLUT1 induction and maintenance factors at the BBB. The immunocytochemical technique used is rapid and requires relatively few cells and may provide a useful adjunct to the more quantitative techniques of Western and Northern blotting.
Hemmila
J M
JM
Department of Biochemistry and Molecular Biology, School of Medicine, University of Minnesota, Duluth 55812.
Drewes
L R
LR
D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology; D001921:Brain / Q000098:blood supply*; D002478:Cells, Cultured; D004285:Dogs; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000166:cytology; D051272:Glucose Transporter Type 1; D007124:Immunoenzyme Techniques; D008833:Microcirculation / Q000737:chemistry; D009004:Monosaccharide Transport Proteins / Q000032:analysis*
D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins