[Inducing ability of co-planar PCBs toward bilirubin UDP-glucuronyltransferase of liver microsomes: the remarkable difference between guinea pigs and rats].
The induction of liver microsomal UDP-glucuronyltransferase (UGT) activity toward bilirubin by pretreatment with 3, 4, 3', 4'-tetrachlorobiphenyl (TCB), 3, 4, 5, 3', 4'-pentachlorobiphenyl (PenCB) and 3-methylcholanthrene (MC) was studied in guinea pigs and rats. In addition, microsomal benzo(a)pyrene 3-hydroxylase and cytosolic DT-diaphorase activities were also measured for the comparison. All of the PenCB, TCB and MC significantly induced the bilirubin UGT activity of guinea pig liver microsomes. The highest induction (6-fold over the control) was seen in the PenCB-treated animal, and MC (3.2-fold) and TCB (2.4-fold) were less effective. On the other hand, the induction of benzo(a)pyrene 3-hydroxylase and DT-diaphorase activities of guinea pigs was not so remarkable as that of bilirubin UGT activity. In the guinea pig, the inducibility of bilirubin UGT activity seemed to be correlated with the toxicity induced with PenCB, TCB and MC. In contrast to the guinea pig, UGT activity toward bilirubin in the rat was significantly decreased by the treatment with the inducers described above. These results suggest that bilirubin UGT activity in the guinea pig can be an index for the toxicity of polychlorinated biphenyls as like as benzo(a)pyrene 3-hydroxylase and DT-diaphorase activities in the rat.
Oguri
K
K
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka.
Koga
Y
Y
Tsuda
M
M
Ariyoshi
N
N
Ishii
Y
Y
Yamada
H
H
Yoshimura
H
H
D000818:Animals; D004790:Enzyme Induction; D014453:Glucuronosyltransferase / Q000096:biosynthesis*; D006168:Guinea Pigs; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology / Q000633:toxicity; D008862:Microsomes, Liver / Q000201:enzymology*; D011078:Polychlorinated Biphenyls / Q000494:pharmacology / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity
D008748:Methylcholanthrene; D011078:Polychlorinated Biphenyls; D014453:Glucuronosyltransferase; C023035:3,4,5,3',4'-pentachlorobiphenyl; C028451:3,4,3',4'-tetrachlorobiphenyl