title: Expression of aphidicolin-induced fragile sites in lymphocytes of patients with breast cancer.
abstract: The expression of fragile sites induced by aphidicolin (APC) was evaluated on metaphase chromosomes obtained from the peripheral blood lymphocytes of 26 women with breast cancer and 15 sex- and age-matched normal controls. Both the proportion of damaged cells (P < 0.001) and the mean number of gaps and breaks per cell (0.02 < P < 0.05) were significantly higher in the patient group. There were no differences in either the age-related fragile site levels or the expression of single fragile sites between patients and controls. Our findings indicate an increased genetic instability in women with breast carcinoma.
authors.affiliation: Istituto di Clinica Medica I, Università di Perugia, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Ardisia
authors.forename: G
authors.initials: G
authors.lastname: Venti
authors.forename: M A
authors.initials: MA
authors.lastname: Colozza
authors.forename: C
authors.initials: C
authors.lastname: Breschi
authors.forename: B
authors.initials: B
authors.lastname: Porfirio
authors.forename: S
authors.initials: S
authors.lastname: Davis
authors.forename: M
authors.initials: M
authors.lastname: Tonato
authors.forename: E
authors.initials: E
authors.lastname: Donti
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D016590:Aphidicolin / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics*; D002478:Cells, Cultured; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008875:Middle Aged
chemical_list: D016590:Aphidicolin