Isolation and sequence of a full length cDNA encoding a novel rat inositol 1,4,5-trisphosphate 3-kinase.
Immunoscreening a rat liver cDNA expression library has led to the isolation of a full-length cDNA clone encoding a novel isoform of rat inositol 1,4,5-trisphosphate 3-kinase (IP3 3-kinase). Sequence comparison shows it (i) to be 93% identical to human hippocampus IP3 3-kinase B over 468 residues at the protein level, and (ii) to encode a protein 204 amino acids larger than the published sequence of its human homologue.
Thomas
S
S
Department of Biochemistry, University of Bristol, UK.
Brake
B
B
Luzio
J P
JP
Stanley
K
K
Banting
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D051381:Rats; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase