title: Mapping the sheep genome: production of characterized sheep x hamster cell hybrids.
abstract: Specific chromosomes of sheep have been selectively "captured" in hamster x sheep cell hybrids produced by fusing different Chinese hamster auxotrophs with lymphocytes from sheep carrying normal or Robertsonian translocation chromosomes. A minipanel has been established comprising sheep chromosomes 1; 3; t1 = rob(6;24); t2 = rob(9;10); and X in a predominantly monochromosomal state. Using this targeted cell fusion approach the nutritional markers, uridine monophosphate synthetase (UMPS), mapped onto human chromosome 3 (HSA3), phosphoribosylglycinamide synthetase, and phosphoribosylaminoimidazole synthetase (GART) (HSA21), are reported to be located on sheep chromosome 1q; and phosphoribosyl pyrophosphate amidotransferase (PPAT) (HSA4) has been assigned to sheep chromosome 6. By isozyme analysis and Southern hybridization, transferrin (TF) and superoxide dismutase 1 (SOD1), both in the bovine syntenic group U10, were assigned to sheep chromosome 1q; adenylate kinase 1 (AK1), in the bovine syntenic group U16, to sheep chromosome 3p; lactate dehydrogenase B (LDHB) and phenylalanine hydroxylase (PAH) on bovine chromosome 5 (BTA5) to sheep chromosome 3q; phosphoglucomutase 2 (PGM2) (bovine syntenic group 15) to sheep chromosome 6 (t1q); avian myelocytomatosis viral oncogene homolog (MYC) and Moloney murine sarcoma viral oncogene homolog (MOS) (BTA 14) to sheep 9 (t2q); and glutathione reductase (GSR) (bovine syntenic group U14) to sheep 24 (t1p).
authors.affiliation: Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado 80206.
authors.forename: D J
authors.initials: DJ
authors.lastname: Burkin
authors.forename: H G
authors.initials: HG
authors.lastname: Morse
authors.forename: T E
authors.initials: TE
authors.lastname: Broad
authors.forename: P D
authors.initials: PD
authors.lastname: Pearce
authors.forename: H A
authors.initials: HA
authors.lastname: Ansari
authors.forename: P E
authors.initials: PE
authors.lastname: Lewis
authors.forename: C
authors.initials: C
authors.lastname: Jones
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004798:Enzymes / Q000235:genetics; D005819:Genetic Markers; D016678:Genome; D006822:Hybrid Cells; D008214:Lymphocytes; D012756:Sheep / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D004798:Enzymes; D005819:Genetic Markers