[Ultrastructural in situ hybridization. A review].
After the first studies of hybridization in 1961, Jacob et al. developed an ultrastructural in situ hybridization technique in 1971. Advances in molecular biology (preparation of probes, labeling techniques...) allowed to refine this method. The radioisotope-labeled probes used initially required prolonged exposure times to achieve autoradiographic detection. Commercially available nonradioactive antigen-labeled nucleotides decreased revelation time and increased signal resolution. The three currently used methods are respectively performed on non-embedded material, pre-embedded material, and material embedded in a hydrophilic resin. Main applications include detection of viral nucleic acids, studies of ribosome maturation, detection of animal and plant mRNAs, and chromosome mapping studies.
Morel
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CNRS URA 1454, Laboratoire d'Histologie-Embryologie, Faculté de Médecine Lyon-Sud, Oullins, France.
D017403:In Situ Hybridization / Q000379:methods*; D066298:In Vitro Techniques; D008854:Microscopy, Electron
Hybridation in situ ultrastructurale. Une revue.