Characterization of proteinases in extracts of adult Haemonchus contortus, the ovine abomasal nematode.
The degradation of several protein substrates, including the blood proteins haemoglobin, albumin and fibrinogen, by proteinases present in extracts of adult Haemonchus contortus was examined over a broad pH range. These proteinases were further characterized on the basis of substrate specificity, inhibitor sensitivity and molecular size by spectrophotometric and substrate gel analysis. The majority of the proteinases capable of degrading the blood proteins tested were active at acidic pH and could be ascribed to the cysteine proteinase class. In addition, evidence is presented that these proteinases are differentially recognized and inhibited by immune sera and that parasites capable of withstanding protective host immune responses exhibit modified expression of proteinases.
Knox
D P
DP
Moredun Research Institute, Edinburgh.
Redmond
D L
DL
Jones
D G
DG
D000018:Abomasum / Q000469:parasitology; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000494:pharmacology; D010450:Endopeptidases / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology; D005340:Fibrinogen / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D006188:Haemonchiasis / Q000276:immunology; D006189:Haemonchus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000494:pharmacology; D012756:Sheep; D013379:Substrate Specificity
D000909:Antibodies, Helminth; D011480:Protease Inhibitors; D005780:Gelatin; D005340:Fibrinogen; D010450:Endopeptidases