Malathion disposition in dermally and orally treated rats and its impact on the blood serum acetylcholine esterase and protein profile.
14C-methoxy-malathion with either pure or 50% E.C. formulated malathion were applied orally or dermally at one tenth of their LD50 to two batches of male albino rats. More than 90% of 14C was released with urine after 24 hours. The rest of 14C was detected in the feces, blood, intestines, liver and kidney in a descending order. No significant 14C was detected in other organs. Comparing the oral pure and formulated malathion treatments, there was no significant variation in the rate of disposition or excretion of 14C-malathion. However, the dermal treatment revealed that the 14C-formulated malathion was released faster than the pure one in urine in the first 24 hours; while the 14C-pure malathion showed relatively higher levels in the feces and blood in the first 24 hours. In a third batch of male albino rats, the effect of the same level of dermal treatment by either pure or 50% E.C. formulated malathion on serum acetylcholine-esterase (A. Ch. E.) activity and serum protein profile was studied. The serum A. Ch. E. activity was found to be inhibited to 40% activity after 6 to 24 hours for both treatments. However, after 96 hours the serum of the pure malathion treated rats showed full recovery of A.Ch.E. activity, while the formulated malathion treated showed only 60% activity. The SDS-PAGE analysis showed a differentiation in the serum protein bands of the 48 hours exposed rats to formulated malathion which was confirmed by the scanned gel profile. The FPLC integrated chromatograms proved an initiation of a new protein band accompanied with rearrangement of the albumin and pre-albumin bands. Thus it can be concluded that, the impact on the blood serum protein profile and A. Ch. E. activity can be used as reliable criteria to detect acute toxicity of malathion and other choline-esterase inhibitors in exposed field workers. Further research is needed to elucidate the specificity and sensitivity of such criteria as biomarkers for human exposure.
Abou Zeid
M M
MM
Zoology Dept., Fac. of Sci, Al-Azhar Univ., Naser-City, Cairo, Egypt.
el-Barouty
G
G
Abdel-Reheim
E
E
Blancato
J
J
Dary
C
C
el-Sebae
A H
AH
Saleh
M A
MA
D000110:Acetylcholinesterase / Q000097:blood / Q000187:drug effects*; D000279:Administration, Cutaneous; D000284:Administration, Oral; D000818:Animals; D005243:Feces / Q000737:chemistry; D008294:Malathion / Q000008:administration & dosage / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics* / Q000633:toxicity / Q000652:urine; D008297:Male; D051381:Rats; D012709:Serum Albumin / Q000187:drug effects*; D014018:Tissue Distribution
D012709:Serum Albumin; D000110:Acetylcholinesterase; D008294:Malathion