7,8-Dihydro-8-oxo-2'-deoxyguanosine present in DNA is not simply an artefact of isolation.
7,8-Dihydro-8-oxo-2'-deoxyguanosine (8-oxodG) is present in DNA isolated from human and murine cells. Other studies have reported artificially elevated levels in DNA extracted using phenol-based methods. This report shows that DNA, isolated by different methods, with or without the use of a phenol reagent, contains similar levels of 8-oxodG. This, taken with other studies of human tissues which show varying levels of 8-oxodG, indicates that, with due care in the extraction procedure, biologically significant amounts of this altered base are present in mammalian DNA.
Harris
G
G
London Hospital Medical College, University of London, UK.
Bashir
S
S
Winyard
P G
PG
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D016477:Artifacts; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D051379:Mice; D008808:Mice, Inbred CBA
D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine