Formation of 2,5-diamino-4-hydroxy-6-(2'-deoxyribosyl)-formamidopyrimidine from 2'-deoxyguanosine in aqueous solution irradiated with 60Co gamma-rays.
2'-Deoxyguanosine in aqueous solution (5 x 10(-4) mol/dm3) was irradiated with 60Co gamma-rays under N2O. Products were separated by gel chromatography (Cellulofine GC-15-m) and high performance liquid chromatography (Inertsil ODS-2). 2-Amino-4-hydroxy-5-formamido-6-(2'-deoxyribosyl)-aminopyrimidine was one of the main radiolytic products. The formation of 2,5-diamino-4- hydroxy-6-(2'-deoxyribosyl)-formamidopyrimidine was detected, this compound being verified by measuring its ultraviolet absorption spectrum and analyzing its trimethylsilylated derivative by gas chromato-mass spectrometry. Its yield in terms of 2-amino-4-hydroxy-5-formamido-6-(2'-deoxyribosyl)- aminopyrimidine was 1:3.
Ali
M
M
Department of Biology, Faculty of Sciences, Hiroshima University, Japan.
Yamamoto
O
O
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003037:Cobalt Radioisotopes; D003849:Deoxyguanosine; D005720:Gamma Rays; D011741:Pyrimidine Nucleosides; D011743:Pyrimidines / Q000302:isolation & purification; D011854:Radiochemistry; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014867:Water
D003037:Cobalt Radioisotopes; D011741:Pyrimidine Nucleosides; D011743:Pyrimidines; D012996:Solutions; D014867:Water; C081940:2,5-diamino-4-hydroxy-6-(2'-deoxyribosyl)-formamidopyrimidine; D003849:Deoxyguanosine