title: Extracorporeal enzymatic removal of low density lipoproteins in rabbits: efficacy and safety.
abstract: An extracorporeal circuit incorporating a plasma separator reactor (PSR) was designed to modify low density lipoproteins (LDL). The PSR was tested in vivo with hypercholesterolemic New Zealand White rabbits. The bioreactor enzymatically converts LDL to a form that can be removed by the body at an enhanced rate. The physiological response of hypercholesterolemic New Zealand White rabbits to 90 minute extracorporeal treatments was monitored. The total plasma cholesterol concentration in the treated rabbits fell sharply (up to 40% decrease) during and following the treatment. Results of safety tests indicate no significant enzyme leaching from the device, no disruption or damage to erythrocytes, no increase in white blood cell count and no liver damage as indicated by five enzyme assays. All safety measurements suggest that the treatment is safe.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge.
authors.forename: S D
authors.initials: SD
authors.lastname: Shefer
authors.forename: J
authors.initials: J
authors.lastname: Ferreira
authors.forename: C J
authors.initials: CJ
authors.lastname: Mullon
authors.forename: R
authors.initials: R
authors.lastname: Langer
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood; D004800:Enzymes, Immobilized; D005112:Extracorporeal Circulation; D006937:Hypercholesterolemia / Q000097:blood / Q000628:therapy*; D008077:Lipoproteins, LDL / Q000097:blood*; D010741:Phospholipases A; D011817:Rabbits
chemical_list: D004800:Enzymes, Immobilized; D008077:Lipoproteins, LDL; D002784:Cholesterol; D010741:Phospholipases A