Determination of khellin in serum by gas chromatography.
A rapid gas chromatographic assay has been developed for the separation and determination of khellin in human serum. Using a DB-17 capillary column and a simple chloroform extraction, khellin and an internal standard, trioxsalen, were separated from endogenous substances without further clean-up. Spiked serum samples in the range 0.11-1.1 micrograms/ml were assayed and a linear calibration curve obtained.
Carlin
A S
AS
Biopharmaceutics Research Branch, Food and Drug Administration, Washington, DC 20204.
Simmons
J E
JE
el-Arini
S K
SK
Shiu
G K
GK
D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007666:Khellin / Q000097:blood*; D011786:Quality Control; D014307:Trioxsalen
D007666:Khellin; D014307:Trioxsalen