Immunoglobulin in the eggs of the channel catfish (Ictalurus punctatus).
Egg yolk proteins obtained from 2-3-day-old fertilized channel catfish eggs were analyzed to determine if immunoglobulin (Ig) was present. Using both monoclonal and polyclonal antibodies to catfish Ig, egg Ig was isolated and structurally characterized. The egg Ig when analyzed in SDS-gels under nonreducing conditions dissociated into eight distinct subpopulations with relative mobilities identical to that previously described for the serum Ig of the channel catfish. In addition, the component heavy (H) and light (L) chains of the egg Ig had similar relative mobilities as the H and L chains identified in serum Ig. Immunocytological studies using both polyclonal and monoclonal anti-catfish Ig showed that Ig was dispersed throughout the yolk of the egg. In addition these analyses indicated that Ig was localized within the external membranes (eggshell) of the egg.
Hayman
J R
JR
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216.
Lobb
C J
CJ
D000818:Animals; D004527:Egg Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D007059:Ictaluridae / Q000276:immunology*; D007136:Immunoglobulins / Q000737:chemistry / Q000302:isolation & purification*; D007150:Immunohistochemistry; D010063:Ovum / Q000276:immunology*; D011487:Protein Conformation; D015053:Zygote / Q000276:immunology
D004527:Egg Proteins; D007136:Immunoglobulins