Evidence for a multi-allelic heterokaryon incompatibility (het) locus detected by hybridization among three heterokaryon-compatibility (h-c) groups of Aspergillus nidulans.
A strain of heterokaryon-compatibility (h-c) group A was crossed sexually to strains of h-cB and h-cGL of Aspergillus nidulans. A back-crossing programme established that there were seven hetero-allelic heterokaryon compatibility (het) genes controlling somatic incompatibility between strains of h-cA and h-cB. A similar back-crossing programme between strains of h-cA and h-cGL confirmed that these two groups differ at six het loci. Previous work has shown that h-cB differs from h-cGL at two het loci, hetA on linkage group V and hetB on linkage group VI. As an allelic difference at a single het locus is enough to cause two strains to be heterokaryon incompatible, 15 alleles spread over seven het loci are necessary to explain the h-cA, h-cB, h-cGL triangular compatibility relationship. One het locus is multi-allelic and this locus must be either hetA or hetB.
Dales
R B
RB
School of Biological Sciences, University of Birmingham, U.K.
Moorhouse
J
J
Croft
J H
JH
D000483:Alleles; D001233:Aspergillus nidulans / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D005800:Genes, Fungal; D006824:Hybridization, Genetic; D013172:Spores, Fungal