[Development of polymers for high performance ion-exchange chromatography and their application to analysis of human serum albumin components].
The versatility of 4-vinylpyridinium (4VP-EG-Q) and quaternized dimethylaminomethylstyrene (PDAMS-Q) polymers synthesized by suspension copolymerization as column packings for high performance ion-exchange chromatography was described. The proposed polymer columns afforded resolution of human/bovine serum albumin components, such as mercaptalbumin and nonmercaptalbumin. It was suggested that the physicochemical structure on the external surface of the ion-exchangers was very important in the resolution. The characteristics of 4VP-EG-Q column were effectively applied to the investigation of the metal-albumin complexes, whereas a rapid analysis of mercaptalbumin in the human serum using the PDAMS-Q column was performed by isocratic elution.
Sugii
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Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
D002852:Chromatography, Ion Exchange; D006801:Humans; D011108:Polymers / Q000138:chemical synthesis*; D011137:Polystyrenes / Q000138:chemical synthesis*; D011725:Pyridines / Q000138:chemical synthesis*; D012709:Serum Albumin / Q000032:analysis* / Q000302:isolation & purification; D000075462:Serum Albumin, Human
C082329:PDAMS-Q polymer; D011108:Polymers; D011137:Polystyrenes; D011725:Pyridines; D012709:Serum Albumin; C001702:mercaptoalbumin; C079732:nonmercaptalbumin; C029351:4-vinylpyridine; D000075462:Serum Albumin, Human