title: Cloning and expression of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase.
abstract: Here we report the cDNA sequence of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase. The cDNA was isolated from a human placental cDNA library by screening with a 150bp probe generated by PCR using degenerate primers based on the sequences found in the sialyl motif. Comparative analysis of this cDNA with the rat liver alpha 2,3-sialyltransferase sequence indicates 91% nucleotide similarity between the two sequence in the predicted coding region. On the amino acid level, the degree of conservation is 97%. Surprisingly, Northern analysis indicated that the gene is expressed at low levels in human placenta but is abundantly expressed in skeletal muscle and fetal tissues.
authors.affiliation: Cytel Corporation, San Diego, California.
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: J C
authors.initials: JC
authors.lastname: Paulson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002240:Carbohydrate Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012799:Sialyltransferases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014162:Transfection; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D012799:Sialyltransferases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase