[The quantitative assay of (1-->3)-beta-D-glucan in culture medium of Candida albicans using G-test].
It has been difficult to diagnose the deep-seated fungal infection. Limulus test which originally has been developed to detect endotoxin in blood is also activated by (1-->3)-beta-D-glucan, the cell wall component of the fungi. Factor G in limulus lysate is activated by (1-->3)-beta-D-glucan and not by endotoxin. The quantitative assay of (1-->3)-beta-D-glucan is possible by the G-test using factor G. (1-->3)-beta-D-glucan in RPMI culture medium of Candida albicans was periodically measured using G-test and the effect of antifungal drug or neutrophil to the changes of (1-->3)-beta-D-glucan in the culture medium was studied. Increase in the level of (1-->3)-beta-D-glucan was in parallel with the growth of Candida albicans. G-test may be applied to the clinical diagnosis of fungal infection.
Kato
H
H
Department of Surgery II, Nagoya University School of Medicine, Japan.
Nakao
A
A
Kambe
T
T
Tanaka
K
K
Tamura
H
H
Tanaka
S
S
Takagi
H
H
D002176:Candida albicans / Q000254:growth & development*; D002177:Candidiasis / Q000175:diagnosis; D003470:Culture Media / Q000032:analysis*; D005936:Glucans / Q000032:analysis*; D006801:Humans; D008033:Limulus Test / Q000379:methods*; D047071:beta-Glucans
D003470:Culture Media; D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan