Natural and synthetic estrogens and prostacyclin production in human endothelial cells from umbilical cord and leg veins.
We have evaluated the effects of 17 beta-estradiol and 17 alpha-ethinylestradiol on prostacyclin production (measured as prostaglandin-6-keto-F1 alpha) in human endothelial cell cultures that had been obtained from umbilical cord and from leg veins. Endothelial cultures were treated with the estrogens at 3 different concentrations (10(-6), 10(-7), 10(-8) M) for up to 72h. 17 beta-estradiol did not affect basal 6-keto-PGF1 alpha production in these cell cultures. With 17 alpha-ethinylestradiol the synthesis of 6-keto-PGF1 alpha was not statistically significant changed. At least in our in vitro system we could not find an effect of natural and synthetic estrogens on 6-keto-PGF1 alpha production. This does, of course not rule out an effect in the more complex in vivo-situation.
Muck
A O
AO
Department of Obstetrics and Gynecology, University of Tubingen.
Seeger
H
H
Korte
K
K
Dartsch
P C
PC
Lippert
T H
TH
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D006801:Humans; D007866:Leg / Q000098:blood supply; D014471:Umbilical Veins / Q000378:metabolism; D014680:Veins / Q000378:metabolism
D004997:Ethinyl Estradiol; D004958:Estradiol; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol