The effect of N-myc amplification and expression on invasiveness of neuroblastoma cells.
Most neuroblastomas with N-myc amplification, a sign of extremely poor prognosis, are extensively invasive to surrounding tissues. Therefore, we investigated the relationship between N-myc amplification and invasiveness of neuroblastoma cells, using an in-vitro assay system. Five human neuroblastoma cell lines were used for this study. IMR-32, GOTO, and DZ, all of which had N-myc amplification, showed a highly invasive capacity. In contrast, SK-N-SH without N-myc amplification showed extremely low invasiveness. ST unexpectedly showed high invasiveness in spite of the lack of N-myc amplification. Treatment of GOTO with 10(-5) mol/L all-trans-retinoic acid (RA) for 72 hours markedly decreased both N-myc expression and invasiveness. Treatment of GOTO with 2 mmol/L dibutyryl cyclic AMP (dbcAMP) for 72 hours caused a slight decrease of N-myc expression and invasiveness. These results indicate that N-myc amplification and expression might be closely related to the invasiveness of human neuroblastoma cells.
Zaizen
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Y
Department of Pediatric Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Taniguchi
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Noguchi
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Suita
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S
D002465:Cell Movement / Q000235:genetics; D005784:Gene Amplification; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D014407:Tumor Cells, Cultured