DNA adducts in peripheral blood lymphocytes from aluminum production plant workers determined by 32P-postlabeling and enzyme-linked immunosorbent assay.
32P-Postlabeling analysis and enzyme-linked immunosorbent assay (ELISA) have been used to detect DNA adducts in peripheral blood lymphocytes from primary aluminum production plant workers who were exposed occupationally to a mixture of polycyclic aromatic hydrocarbons (PAHs). Preliminary results reported here are from a comparative study being performed in two aluminum plants. The levels of aromatic DNA adducts have been determined by the 32P-postlabeling assay in samples collected on two occasions, 1 year apart. PAH-DNA adduct levels have also been determined by competitive ELISA in the second set of DNA samples. The results show the necessity of follow-up biomonitoring studies to detect possible alterations in biological effect induced by changing exposures. The comparison of the results obtained by 32P-postlabeling and ELISA may lead to a better understanding of the power and weaknesses of the two methods applied in these studies.
Schoket
B
B
Department of Biochemistry, Johan Bela National Institute of Public Health, Budapest, Hungary.
Phillips
D H
DH
Poirier
M C
MC
Vincze
I
I
D000535:Aluminum; D004247:DNA / Q000097:blood* / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D016273:Occupational Exposure; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds / Q000009:adverse effects* / Q000097:blood
D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds; D004247:DNA; D000535:Aluminum