title: Gas chromatographic/chemiluminescence detection (thermal energy analyzer-nitrogen mode) method for the determination of dibutylamine in hams.
abstract: A gas chromatographic (GC) procedure is described for the determination of dibutylamine (DBA) in ham at less than 1 ppm. Sample is extracted with 0.5N HCl, centrifuged, made basic, and then extracted with isopropyl alcohol. Final determination is by GC/thermal energy analysis in the nitrogen mode. Six replicate analyses of ham fortified with 0.5 ppm DBA gave a recovery of 90.2 +/- 3.4%. Over a range of 0.5 to 3.0 ppm DBA, the repeatability was 0.07 ppm, and the coefficient of variation was 6.07%. Limit of detection was 0.1 ppm. Effectiveness of the procedure was demonstrated on selected ham samples.
authors.affiliation: U.S. Department of Agriculture, Eastern Regional Research Center, Philadelphia, PA 19118.
authors.forename: W
authors.initials: W
authors.lastname: Fiddler
authors.forename: R C
authors.initials: RC
authors.lastname: Doerr
mesh_terms: D000818:Animals; D002082:Butylamines / Q000032:analysis*; D002849:Chromatography, Gas; D004055:Differential Thermal Analysis; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008460:Meat / Q000032:analysis*; D013552:Swine
chemical_list: D002082:Butylamines; D007202:Indicators and Reagents; C045274:dibutylamine