Determination of chloroacetaldehyde, a metabolite of oxazaphosphorine cytostatic drugs, in plasma.
A derivatization high-performance liquid chromatographic method with ultraviolet detection to monitor the plasma concentration of chloroacetaldehyde, a neurotoxic metabolite of oxazaphosphorine drugs, is presented. To prevent the rapid degradation of chloroacetaldehyde, the plasma samples are stabilized with formaldehyde. The method is linear in the concentration range 1-250 nmol/ml. Blood samples from a patient who was treated with a ten-day continuous infusion of ifosfamide were assayed. The chloroacetaldehyde concentrations did not exceed 10 nmol/ml.
Kaijser
G P
GP
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, Netherlands.
Beijnen
J H
JH
Jeunink
E L
EL
Bult
A
A
Keizer
H J
HJ
de Kraker
J
J
Underberg
W J
WJ
D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000097:blood; D000368:Aged; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005557:Formaldehyde; D006801:Humans; D007069:Ifosfamide / Q000493:pharmacokinetics*
D005557:Formaldehyde; C004656:chloroacetaldehyde; D000079:Acetaldehyde; D007069:Ifosfamide