Identification of Escherichia coli serotype O157:H7 by DNA probe specific for an allele of uid A gene.
Isolates of Escherichia coli serotype O157:H7 were identified by an oligonucleotide probe, PF-27, containing a unique base substitution in the allele of the uid A gene. Colony hybridization analysis of 239 bacteria, including E. coli, Shiga-like toxin-producing serogroups of pathogenic E. coli and other enteric isolates showed that the probe reacted only with isolates of serotype O157:H7. Results of genetic analyses suggest that the base substitution in the allele does not contribute to the absence of uid A gene expression in O157:H7.
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Division of Microbiological Studies, Food and Drug Administration, Washington, DC 20204.
D000483:Alleles; D001419:Bacteria / Q000235:genetics; D015373:Bacterial Typing Techniques; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D005516:Food Microbiology; D005798:Genes, Bacterial; D006801:Humans; D012703:Serotyping
D015342:DNA Probes; D004269:DNA, Bacterial