Quantification of serum 1,5-anhydroglucitol in uremic and diabetic patients by liquid chromatography/mass spectrometry.
We developed a new method for measuring serum 1,5-anhydroglucitol (1,5-AG), using liquid chromatography/atmospheric pressure chemical-ionization mass spectrometry (LC/MS). With this method we measured serum 1,5-AG concentrations in uremic and diabetic patients and compared these values with those determined by an enzymatic method. Serum 1,5-AG concentrations were significantly less in the undialyzed and dialyzed uremic patients and in diabetic patients; the values in the dialyzed patients decreased after hemodialysis. In uremic patients with high concentrations of serum myo-inositol, the 1,5-AG values determined by LC/MS were significantly lower than those determined enzymatically. There was no significant correlation between 1,5-AG and fructosamine in the uremic patients. These results demonstrate that serum 1,5-AG cannot be used as an index for glycemic control in uremic patients and that the LC/MS method is indicated in uremic patients with high concentrations of myo-inositol.
Niwa
T
T
Nagoya University Branch Hospital, Japan.
Dewald
L
L
Sone
J
J
Miyazaki
T
T
Kajita
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002853:Chromatography, Liquid / Q000379:methods* / Q000706:statistics & numerical data; D003847:Deoxyglucose / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D019270:Fructosamine; D006595:Hexosamines / Q000097:blood; D006801:Humans; D007294:Inositol / Q000097:blood; D008297:Male; D013058:Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D008875:Middle Aged; D012016:Reference Values; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood* / Q000628:therapy
D006595:Hexosamines; D019270:Fructosamine; D007294:Inositol; C006584:1,5-anhydroglucitol; D003847:Deoxyglucose