Platelet changes in classic migraine.
We investigated some ultrastructural aspects of platelet activity in patients with classic migraine during an attack-free period. We focused on the production of malondialdehyde (MDA), the intracellular concentration of Ca++ and the membrane phospholipid structure using a DPH (1,6 diphenyl-1,3,5 hexatriene) fluorescent probe. Our study comprised 20 patients with classic migraine and 5 healthy controls, from whom 10 cc samples of blood were collected in test tubes containing sodium citrate (1/9 cc). We found no significant differences in MDA production between migraineurs and controls either before or after stimulation with thrombin. The same applies to the concentration of intracellular Ca++ (Ca++i). The membrane lipid composition assessed by DPH fluorescent probe was normal both in migraineurs and in controls. Our findings suggest no intrinsic activation of platelet aggregation.
De Matteis
G
G
Centro Cefalee, Università dell'Aquila.
Tozzi-Ciancarelli
M G
MG
Calisse
P
P
Totaro
R
R
D'Andrea
F
F
Di Massimo
C
C
Prencipe
M
M
D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D005260:Female; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D008875:Middle Aged; D008881:Migraine Disorders / Q000097:blood*; D009994:Osmolar Concentration
D008563:Membrane Lipids; D008315:Malondialdehyde; D002118:Calcium