title: In vitro spontaneous production of anti-SIV antibodies is a reliable tool in the follow-up of protection of SIV-vaccinated monkeys.
abstract: To assess the reliability of the spontaneous in vitro synthesis of simian immunodeficiency virus (SIV)-specific antibodies as a marker in the monitoring of protection in SIV-vaccinated animals, Macaca fascicularis monkeys were immunized with formalin-inactivated SIVmac251 or SIVmac251/32H, and challenged with human-derived (SIVmac251/32H) or monkey-derived live SIV. As judged by virus isolation and polymerase chain reaction (PCR) techniques, immunized animals were protected against human-derived SIV challenge, and no spontaneous in vitro synthesis of anti-SIV antibody was observed in nonstimulated peripheral blood mononuclear cell cultures over a 4-month follow-up. On the contrary, human cell-grown SIVmac251 immunization did not afford protection against monkey-derived SIV, and all the animals became infected and showed spontaneous in vitro synthesis of anti-SIV antibodies. These data demonstrate that lack of protection in SIV-vaccinated monkeys is strictly associated with PBMC ability of spontaneously produce anti-SIV antibodies in vitro following challenge, and suggest that this parameter might also constitute a reliable marker for monitoring protection in large-scale HIV vaccination and immunotherapy programs.
authors.affiliation: Institute of Oncology, University of Padova, Padua, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Zamarchi
authors.forename: M L
authors.initials: ML
authors.lastname: Veronese
authors.forename: F
authors.initials: F
authors.lastname: Titti
authors.forename: A
authors.initials: A
authors.lastname: Geraci
authors.forename: P
authors.initials: P
authors.lastname: Verani
authors.forename: G B
authors.initials: GB
authors.lastname: Rossi
authors.forename: A
authors.initials: A
authors.lastname: Amadori
authors.forename: L
authors.initials: L
authors.lastname: Chieco-Bianchi
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008252:Macaca fascicularis; D011043:Pokeweed Mitogens / Q000494:pharmacology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000494:pharmacology; D014765:Viral Vaccines / Q000494:pharmacology*
chemical_list: D000914:Antibodies, Viral; D011043:Pokeweed Mitogens; D015164:Vaccines, Inactivated; D014765:Viral Vaccines