title: Workshop on in vitro neutralization of Chlamydia trachomatis: summary of proceedings.
abstract: A task force evaluated an in vitro antibody-mediated chlamydial neutralization assay for its utility as a method to assess functional correlates of antibody responses to Chlamydia trachomatis. Two monoclonal antibodies that recognize different major outer membrane protein (MOMP) epitopes for a C. trachomatis serovar B strain exhibit good in vitro neutralizing activity, with a maximum of 90% neutralization. Calculations based on the 50% neutralization point indicated that 100% neutralization could theoretically be achieved when only 10% of the MOMP molecules bound antibody. Monoclonal antibodies that recognized either a heterologous MOMP or the genus-specific chlamydial lipopolysaccharide did not produce neutralizing activity. The standardized assay will be useful to establish if in vitro neutralizing antibody responses are predictive of protective immunity and will aid in defining chlamydial antigens and epitopes that may be attractive vaccine candidates.
authors.affiliation: Dept. of Medical Microbiology, University of Wisconsin, Madison 53706.
authors.forename: G I
authors.initials: GI
authors.lastname: Byrne
authors.forename: R S
authors.initials: RS
authors.lastname: Stephens
authors.forename: G
authors.initials: G
authors.lastname: Ada
authors.forename: H D
authors.initials: HD
authors.lastname: Caldwell
authors.forename: H
authors.initials: H
authors.lastname: Su
authors.forename: R P
authors.initials: RP
authors.lastname: Morrison
authors.forename: B
authors.initials: B
authors.lastname: Van der Pol
authors.forename: P
authors.initials: P
authors.lastname: Bavoil
authors.forename: L
authors.initials: L
authors.lastname: Bobo
authors.forename: S
authors.initials: S
authors.lastname: Everson
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002460:Cell Line; D002692:Chlamydia trachomatis / Q000276:immunology*; D006224:Cricetinae; D000882:Haplorhini; D006801:Humans; D008647:Mesocricetus; D009500:Neutralization Tests; D014141:Trachoma / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial