title: DNA replication of parvovirus B 19 in a human erythroid leukemia cell line (JK-1) in vitro.
abstract: A major limitation of studies on the parvovirus B 19, a causative agent of transient aplastic crisis, has been the absence of appropriate cell lines permissive for the virus. In the present study, a human erythroid leukemia cell line (JK-1) was shown to support B 19 virus DNA replication in vitro. Forty-eight hours after virus inoculation of JK-1 liquid cell cultures, the average number of B 19 genome copies was estimated at 3,000 per cell by DNA dot blot analysis. The addition of erythropoietin increased B 19 copy number to 10,000 per cell. The presence of replicative forms of the B 19 virus genome was genome was demonstrated by Southern blot analysis. Although persistent infection of B 19 virus was not observed in JK-1 cells, this culture system will be of value in elucidating the molecular basis of the erythroid specificity of parvovirus B 19.
authors.affiliation: Department of Hematology-Oncology, Institute of Medical Science, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Ozawa
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Okuno
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Muto
authors.forename: F
authors.initials: F
authors.lastname: Takaku
authors.forename: S
authors.initials: S
authors.lastname: Asano
mesh_terms: D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D016732:Parvovirus B19, Human / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D004279:DNA, Viral