title: Minimal residual disease in vitro of a pre-B-lymphoma.
abstract: We have studied the survival of clonogenic neoplastic cells of a murine pre-B-lymphoma (BCl-1) of the spleen in culture. We have found quantitative deficiencies such as reduced surface adherence of stromal cells and impaired CFU-F (colony forming units-fibroblast) and pre-CFU-F colony and layer formation in stromal cultures of lymphoma bearing spleen, as compared to cultures from normal spleen. There are two populations of clonogenic BCl-1 lymphoma cells surviving in culture: one population is surface adherent, and the other is non-adherent. Both populations transmit the lymphoma to healthy indicator mice. We hope that this model will be helpful in studying minimal residual leukemic disease [1] in culture.
authors.affiliation: Department of Pediatrics, University Medical School Göttingen, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Prindull
authors.forename: Z
authors.initials: Z
authors.lastname: Ben-Ishay
authors.forename: M
authors.initials: M
authors.lastname: Bergholz
authors.forename: B
authors.initials: B
authors.lastname: Prindull
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival; D002999:Clone Cells; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000473:pathology; D017154:Stromal Cells / Q000473:pathology; D014407:Tumor Cells, Cultured