The uptake of manganese dipyridoxal-diphosphate by chemically induced hepatocellular carcinoma in rats. A correlation between contrast-media-enhanced magnetic resonance imaging, tumor differentiation, and vascularization.
OBJECTIVE
Negative enhancement of implanted liver tumors has been achieved in preclinical studies on manganese dipyridoxal-diphosphate (Mn-DPDP), a new hepatobiliary specific contrast agent for magnetic resonance imaging (MRI). The authors investigated the effects of Mn-DPDP on primary liver cancer and its possible mechanisms.


METHODS
Magnetic resonance imaging was performed in 15 rats with chemically induced hepatocellular carcinoma (HCC) before and after Mn-DPDP injection. Both tumor-liver contrast-to-noise ratio and absolute tumor enhancement were evaluated and compared with the MRI results of a nonspecific contrast agent, gadolinium-DOTA, and correlated with corresponding microangiographic and histologic findings.


RESULTS
Mn-DPDP injection led to a persistent positive enhancement in differentiated solid HCCs (22/23) with maximal conspicuity at 24 hours. Undifferentiated HCCs were all delineated by a prompt negative enhancement (20/20) with maximal conspicuity within 30 minutes.
Ni
Y
Y
Department of Radiology, University Hospitals K.U., Leuven, Belgium.
Marchal
G
G
Zhang
X
X
Van Hecke
P
P
Michiels
J
J
Yu
J
J
Rummeny
E
E
Lodemann
K P
KP
Baert
A L
AL
D000792:Angiography; D000818:Animals; D003287:Contrast Media; D004492:Edetic Acid / Q000031:analogs & derivatives*; D006571:Heterocyclic Compounds; D008114:Liver Neoplasms, Experimental / Q000098:blood supply / Q000175:diagnosis* / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male; D008852:Microradiography; D009942:Organometallic Compounds; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives*; D051381:Rats; D017208:Rats, Wistar
D003287:Contrast Media; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D011732:Pyridoxal Phosphate; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; D004492:Edetic Acid; C060076:N,N'-bis(pyridoxal-5-phosphate)ethylenediamine-N,N'-diacetic acid