title: Detection of specific glucose-3-phosphatase activity in rat liver.
abstract: Sugar-3-phosphates are related to aspects of diabetes which depend on protein glycosylation events. Sorbitol-3-phosphate and fructose-3-phosphate occur in normal and diabetic individuals, and glucose-3-phosphate is a potential intermediate in their biosynthesis. Almost nothing is known about enzyme pathways for their metabolic turnover. We have found that part of the phosphohydrolytic activity on glucose-3-phosphate in rat liver supernatants corresponds to a specific, Mg(2+)-dependent, glucose-3-phosphatase much less or not active on other phosphate esters, including glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, fructose-6-phosphate and p-nitrophenyl-phosphate. This finding opens a route to a better understanding of the metabolism and role of sugar-3-phosphates.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Canales
authors.forename: F
authors.initials: F
authors.lastname: Buitrago
authors.forename: A
authors.initials: A
authors.lastname: Faraldo
authors.forename: J C
authors.initials: JC
authors.lastname: Cameselle
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D005260:Female; D005958:Glucosephosphates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D015636:Magnesium Chloride / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005958:Glucosephosphates; D015636:Magnesium Chloride; C085852:glucose-3-phosphatase; D010744:Phosphoric Monoester Hydrolases