title: Reversible pseudohyphal growth in haploid Saccharomyces cerevisiae is an aerobic process.
abstract: Pseudohyphal growth in Saccharomyces cerevisiae has been postulated to be an adaptation to foraging for nitrogen during nitrogen starvation. This process was described as a strictly diploid phenomenon which did not occur in haploid yeast cells and was under the genetic control of both the mating-type locus and a group of five genes, the BUD genes, regulating bud formation. We have also observed a dimorphic growth pattern in yeast growing on various nitrogen-limiting synthetic media. However, and in contrast to a previous report, we find that pseudohyphal growth is not precluded in haploid cells. We demonstrate that haploid pseudohyphal growth is strictly oxygen-dependent and is rapidly reversible, defining pseudohyphal growth as a reversible developmental pathway in yeast.
authors.affiliation: Webb-Waring Lung Institute, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: R M
authors.initials: RM
authors.lastname: Wright
authors.forename: T
authors.initials: T
authors.lastname: Repine
authors.forename: J E
authors.initials: JE
authors.lastname: Repine
mesh_terms: D006238:Haploidy; D010100:Oxygen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D010100:Oxygen