[The production and characteristics of polyclonal antibodies to the threonine-containing site of the cytoplasmic domain of the EGF receptor].
A conjugate of a synthetic polypeptide to hemocyanine was used as an antigen for obtaining polyclonal antibodies to the site of cytoplasmic domain of the epidermal growth factor (EGF) receptor. The amino acid sequence of the peptide used was the same as that of the EGF receptor from residue 650 to 661. In the A431 cell solubilizate the obtained antibodies interact with the phosphorylated protein with 170 kDa molecular weight (MW), by immunoblotting recognize the protein of this MW, and as evidenced by immunofluorescence, their distribution in the cell is the same as that of monoclonal antibodies to the EGF receptor. It is concluded that the obtained antibodies may recognize the EGF receptor. Moreover, these antibodies in solubilizates of A431, CHO cells, and normal human fibroblasts by immunoblotting recognize 74 and 76 kDa proteins.
Sorokin
A B
AB
Aleksandrov
K E
KE
Vinogradova
N A
NA
Nesterov
A M
AM
Nikol'skiĭ
N N
NN
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification*; D002478:Cells, Cultured / Q000276:immunology; D006224:Cricetinae; D003593:Cytoplasm / Q000276:immunology; D066246:ErbB Receptors / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007114:Immunization / Q000379:methods; D015151:Immunoblotting / Q000379:methods; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011233:Precipitin Tests / Q000379:methods; D011817:Rabbits; D013912:Threonine / Q000276:immunology*
D000906:Antibodies; D013912:Threonine; D066246:ErbB Receptors
Poluchenie i kharakteristika poliklonal'nykh antitel k treoninsoderzhashchemu uchastku tsitoplazmaticheskogo domena retseptora EFR.