A human T cell line with an abnormal trisomy 2 karyotype established by coculture of peripheral lymphocytes with an HTLV-II-infected simian leukocyte cell line.
A new human T cell line with a chromosomal abnormality (47,XY,+2), designated AS-IIA, was established by coculturing peripheral blood leukocytes of a healthy adult male with a lethally irradiated human T lymphotropic virus type II (HTLV-II)-infected simian leukocyte cell line (Si-IIA). A polymerase chain reaction method showed that this interleukin-2 (IL-2)-dependent cell line possessed the HTLV-II provirus genome; the cells also reacted with HTLV-II-positive human sera, anti-HTLV-I/II p24, and anti-HTLV-II gp46 antibodies. AS-IIA cells expressed the suppressor/cytotoxic T cell markers CD3+, CD4-, CD8+, CD25+, and HLA-DR+, with later conversion to CD8-. These cells showed better proliferation than other human HTLV-II-infected cell lines with normal karyotypes, but were not transplantable into severe combined immunodeficiency mice. Virus production from AS-IIA was confirmed not only by electron microscopic examination, which revealed mature and immature type C virus particles, but also by the capacity of the line to immortalize human T cells. These results suggest that HTLV-II shows broad tropism for T cells including CD4+ or CD8+, and that not only Si-IIA, but also AS-IIA, are good sources of HTLV-II. The authors of the present study believe that AS-IIA may be a useful human T cell line for the investigation of HTLV-II in comparison with HTLV-I.
Ohara
N
N
Second Department of Pathology, Okayama University Medical School, Okayama University, Japan.
Hayashi
K
K
Miyamoto
K
K
Tomita
N
N
Fujiwara
K
K
Kondo
E
E
Takahashi
K
K
Ohtsuki
Y
Y
Akagi
T
T
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D002461:Cell Line, Transformed / Q000473:pathology; D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D002889:Chromosomes, Human, Pair 2; D005260:Female; D015367:Human T-lymphotropic virus 2 / Q000235:genetics; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007621:Karyotyping; D008297:Male; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000187:drug effects / Q000382:microbiology / Q000473:pathology*; D014314:Trisomy; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D007376:Interleukin-2