Cyclin G: a new mammalian cyclin with homology to fission yeast Cig1.
A new gene encoding a cyclin-like protein has been isolated from a rat fibroblast cDNA library by cross-hybridization with a mixture of c-src family proto-oncogene kinase domains as a probe. This putative cyclin, called cyclin G, contains a typical cyclin box at the N-terminus but no apparent 'destruction box' or 'PEST' sequence. Interestingly, in its C-terminus region, it has a sequence homologous with a tyrosine phosphorylation site of the epidermal growth factor receptor. Although this cyclin is phylogenetically related to HCS26 of Saccharomyces cerevisiae, it most resembles Cig1, a B-type cyclin, of Schizosaccharomyces pombe, which has been suggested to act at the G1/S phase of the cell cycle. Cyclin G mRNA is induced within 3 h after growth stimulation and remains elevated with no apparent cell cycle dependency, indicating its close association with growth stimuli but not with the cell cycle.
Tamura
K
K
Department of Molecular Genetics, Osaka University, Japan.
Kanaoka
Y
Y
Jinno
S
S
Nagata
A
A
Ogiso
Y
Y
Shimizu
K
K
Hayakawa
T
T
Nojima
H
H
Okayama
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins / Q000737:chemistry* / Q000235:genetics; D004247:DNA / Q000302:isolation & purification; D005656:Fungal Proteins / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012568:Schizosaccharomyces / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
C534702:CCNG1 protein, human; C534704:Ccng1 protein, rat; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D005656:Fungal Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D004247:DNA