[The inhibitory effect of antisense oligodeoxynucleotides on the growth of human pancreatic adenocarcinoma cell lines].
In order to study the inhibitory effect of antisense oligodeoxynucleotides on the growth of human pancreatic adenocarcinoma cell lines (PC-2 & PC-3), synthesized c-myc & c-Ki-ras antisense oligodeoxynucleotides (ASODN) and sense oligodeoxynucleotides (SODN) were added to the culture media of PC-2 & PC-3 cell lines. The cell growth activities after 24, 48, 72 hours of culture were estimated by cell count and 3H-TdR incorporation, and the concurrent oncogene expressions were studied by adopting reverse transcription PCR technique. Cell growth was significantly inhibited after exposure to ASODN for 24 & 48 hours. Cell growth in the c-Ki-ras ASODN group approached nearly the same level as in the control groups 72 hours after exposure, whereas cell proliferation in the c-myc ASODN medium was still being inhibited. By reverse transcription PCR technique, marked inhibition of c-myc & c-Ki-ras expression was seen in the groups after 24 & 48 hours treatment of c-myc & c-Ki-ras ASODNs. There was also down-regulation of c-myc & c-Ki-ras expression after 72 hours exposure to both oncogene ASODNs.
Liu
T H
TH
Department of Pathology, PUMC Hospital, CAMS, Beijing.
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology*; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D016376:Oligonucleotides, Antisense