DNA fingerprint bands applied to linkage analysis with quantitative trait loci in chickens.
An efficient approach to detect association between quantitative traits and bands of DNA fingerprint patterns uses intra-family tail analysis, which compares fingerprints of DNA mixes from individuals at the two tails of a phenotypic distribution. In analysis of 67 paternal half-sibs of a meat-type chicken family, of 57 sire bands generated by two probes, one sire-specific band (S6.6) was associated with abdominal fat deposition. The band effect was estimated by a linear model analysis to be 0.88 standard deviations, or about 30% of the family mean. The association between band S6.6 and abdominal fat was further examined by testing progeny of paternal half-sibs of the chickens which were used in the tail analysis, establishing genetic linkage between the DNA marker and a genetic locus affecting abdominal fat deposition.
Plotsky
Y
Y
Department of Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Cahaner
A
A
Haberfeld
A
A
Lavi
U
U
Lamont
S J
SJ
Hillel
J
J
D000273:Adipose Tissue / Q000254:growth & development*; D000704:Analysis of Variance; D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000254:growth & development; D003433:Crosses, Genetic; D016172:DNA Fingerprinting / Q000662:veterinary; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D005819:Genetic Markers; D016014:Linear Models; D008297:Male
D005819:Genetic Markers