[PCR-based typing of human buccal cell's DNA extracted from whole saliva and saliva stains].
We examined DNA from human saliva for usage as an individual discrimination material. The whole saliva included buccal cells at 17-500 cells/microliters. The DNA in the saliva was extracted with Chelex-100, and then subjected to PCR (polymerase chain reaction) treatment. The amplification site in the DNA was the D1S80 (MCT118) locus. One microliter of fresh saliva was enough for amplification of the DNA by PCR. The electrophoresis patterns of DNA from saliva were identical with those from blood. The extraction of DNA from saliva stains on cigarette papers was also examined. In most cases (more than 90% of all cigarette papers tested), analysis for DNA typing gave satisfactory results. DNA isolated from saliva stains on human skin also gave satisfactory results. This method is highly reliable and applicalle to legal medicine.
Ohhashi
A
A
Forensic Science Laboratory, Toyama Prefectural Police Headquarters.
Aoki
T
T
Matsugo
S
S
Simasaki
C
C
D000328:Adult; D001483:Base Sequence; D002610:Cheek; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000166:cytology*; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry*
D004247:DNA