The identification, cloning, and sequence analysis of the coat protein coding region of a birch isolate (I2) of cherry leaf roll nepovirus.
The coat protein gene of RNA-2 of cherry leaf roll nepovirus (CLRV) birch isolate I2 was cloned, identified, and sequenced. Transcripts derived from cDNA to the coat protein gene made a polypeptide of M(r) 51.5 k when translated in vitro. The predicted amino acid sequence of the coat protein showed little identity with nepoviruses having small RNA-2s. It did, however, have 27% sequence identity with the coat protein of tomato ringspot nepovirus which, like CLRV, has a relatively large RNA-2.
Scott
N W
NW
Institute of Virology and Environmental Microbiology, Oxford, U.K.
Cooper
J I
JI
Edwards
M L
ML
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D004279:DNA, Viral; D004926:Escherichia coli; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral; D017386:Sequence Homology, Amino Acid
D004279:DNA, Viral; D012367:RNA, Viral