Sporadic amplification of the c-fms proto-oncogene in human musculoskeletal sarcomas.
Fresh samples of bone and soft tissue sarcoma from 31 patients were analyzed by the Southern technique for amplification or other structural abnormalities of the c-fms oncogene. As a sole finding, amplification of the c-fms (4- and 10-fold) was detected in 2 of 3 cases with a histologic diagnosis of liposarcoma. RFLP analysis disclosed no gene rearrangements associated with the c-fms amplification. The case with the highest amplification of the c-fms gene was disease-free at review. The 2 fms-amplified liposarcomas were aneuploid according to DNA flow cytometry. This is the first study demonstrating amplification of the c-fms proto-oncogene in human liposarcoma. The fact that this structural abnormality of the c-fms gene was only detected in liposarcomas might point to an uncommon but tissue-specific phenomenon.
Castresana
J S
JS
Department of Tumor Pathology, Karolinska Hospital, Stockholm, Sweden.
Barrios
C
C
Ruiz
J
J
Gómez
L
L
Kreicbergs
A
A
D015139:Blotting, Southern; D001859:Bone Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005784:Gene Amplification; D016236:Genes, fms / Q000235:genetics*; D006801:Humans; D008080:Liposarcoma / Q000235:genetics; D000090063:Proto-Oncogene Mas; D012509:Sarcoma / Q000235:genetics*; D012983:Soft Tissue Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas