[Dielectric measurements on the rabbit cornea using a surface electrode].
Using a concentric surface electrode, we measured in situ electrical impedance in the 10 kHz-100 MHz range for the anterior portion of eyeballs isolated from white rabbits. Replacement of fluid in the anterior chamber with an equal volume of air led to decreases in conductivity, resulting in a marked alteration of impedance behavior as expressed in a loss tangent plot. Eyeballs either with corneal erosin induced through mechanical abrasion or with endothelial injury caused by a 70% ethanol replacement in the anterior chamber were subjected to impedance measurements. Dielectric analysis based on the two-term Cole-Cole equation revealed that the observed overall dielectric dispersion could be divided into two subdispersions. Of these, the first dispersion, occurring at low frequencies was associated with the endothelial layer and the second, higher-frequency dispersion was due to the epithelial layer of the cornea.
Watanabe
M
M
Department of Ophthalmology, Kochi Medical School, Nankoku, Japan.
Mokudai
Y
Y
Ueno
H
H
Ando
M
M
Irimajiri
A
A
D000818:Animals; D003315:Cornea / Q000502:physiology* / Q000648:ultrastructure; D017097:Electric Impedance; D004566:Electrodes; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D011817:Rabbits