title: Stimulation of pancreatic growth by cholecystokinin is mediated by high affinity receptors on rat pancreatic acinar cells.
abstract: Pancreatic acinar cells possess both high low affinity receptors for cholecystokinin. The cholecystokinin analog caerulein, which exerts a trophic effect on the rat pancreas, acts as an agonist at both types of receptors. In contrast, the synthetic analog CCK-JMV-180, which also acts as an agonist at high affinity receptors, opposes the action of caerulein on the low affinity receptors. We report that infusion of either caerulein or CCK-JMV-180 into rats increases [3H]-thymidine incorporation into pancreatic DNA and causes the pancreatic weight as well as content of DNA, RNA, and protein to increase. CCK-JMV-180 also stimulates in-vitro incorporation of [3H]-thymidine into DNA of cultured rat acini. The finding that both caerulein and CCK-JMV-180 exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell cholecystokinin receptors.
authors.affiliation: Department of Surgery, Beth Israel Hospital/Harvard Medical School, Boston, MA 02215.
authors.forename: R
authors.initials: R
authors.lastname: Dawra
authors.forename: A
authors.initials: A
authors.lastname: Saluja
authors.forename: M M
authors.initials: MM
authors.lastname: Lerch
authors.forename: M
authors.initials: M
authors.lastname: Saluja
authors.forename: C
authors.initials: C
authors.lastname: Logsdon
authors.forename: M
authors.initials: M
authors.lastname: Steer
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002108:Ceruletide / Q000494:pharmacology; D002766:Cholecystokinin / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D007700:Kinetics; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000187:drug effects* / Q000378:metabolism; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D011949:Receptors, Cholecystokinin; D014316:Tritium; C058451:JMV 180; D002108:Ceruletide; D004247:DNA; D002766:Cholecystokinin; D012844:Sincalide; D013936:Thymidine