title: Glycosylation and deglycosylation of proteasomes (prosomes) from calf-liver cells: high abundance of neuraminic acid.
abstract: Proteasomes (prosomes) of calf-liver cells were probed with three different biotinylated lectins: Limulus polyphemus agglutinin (LPA), specific for neuraminic acid; Solanum tuberosum agglutinin (STA), specific for GlcNac; and concanavalin A (Con A), specific for Man/Glc. While only one proteasomal protein reacted with STA, most of the proteasomal proteins reacted with LPA and several with Con A. Deglycosylation with N-glycosidase F showed that the detected glycan residues were asparagine-linked. Finally we demonstrate an alternative method for the isolation of proteasomes based on the affinity of certain proteasomal proteins to Con A.
authors.affiliation: Université Blaise Pascal, Clermont-Fd II, Aubière, France.
authors.forename: H P
authors.initials: HP
authors.lastname: Schmid
authors.forename: R
authors.initials: R
authors.lastname: Vallon
authors.forename: W
authors.initials: W
authors.lastname: Tomek
authors.forename: C
authors.initials: C
authors.lastname: Kreutzer-Schmid
authors.forename: M N
authors.initials: MN
authors.lastname: Pouch
authors.forename: S
authors.initials: S
authors.lastname: Badaoui
authors.forename: G
authors.initials: G
authors.lastname: Boissonnet
authors.forename: M
authors.initials: M
authors.lastname: Briand
authors.forename: Y
authors.initials: Y
authors.lastname: Briand
authors.forename: J
authors.initials: J
authors.lastname: Buri
mesh_terms: D000818:Animals; D060829:Arthropod Proteins; D001216:Asparagine / Q000032:analysis; D001665:Binding Sites; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D037102:Lectins / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D009438:Neuraminic Acids / Q000032:analysis*; D037121:Plant Lectins; D011134:Polysaccharides / Q000032:analysis; D046988:Proteasome Endopeptidase Complex
chemical_list: D060829:Arthropod Proteins; D037102:Lectins; D009097:Multienzyme Complexes; D009438:Neuraminic Acids; D037121:Plant Lectins; D011134:Polysaccharides; C041202:potato lectin; D003208:Concanavalin A; D001216:Asparagine; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex