Molecular characterization of a swelling-induced chloride conductance regulatory protein, pICln.
Cells maintain control of their volume by the passage of KCl and water across their membranes, but the regulatory proteins are unknown. Expression in Xenopus oocytes of a novel protein, pICln, activated a chloride conductance. We have cloned analogs of pICln from rat heart and Xenopus ovary. pICln was identified as an abundant soluble cytosolic protein (approximately 40 kd) that does not immunolocalize with the plasma membrane. pICln was found in epithelial and cardiac cells, brain, and Xenopus oocytes, forming complexes with soluble actin and other cytosolic proteins. Monoclonal antibodies recognizing pICln blocked activation of a native hypotonicity-induced chloride conductance (ICl.swell) in Xenopus oocytes, suggesting that pICln may link actin-bound cytoskeletal elements to an unidentified volume-sensitive chloride channel. The high degree of sequence conservation and widespread expression of pICln suggest that it is an important element in cellular volume regulation.
Krapivinsky
G B
GB
Department of Pharmacology, Mayo Foundation, Rochester, Minnesota 55905.
Ackerman
M J
MJ
Gordon
E A
EA
Krapivinsky
L D
LD
Clapham
D E
DE
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D018118:Chloride Channels / Q000096:biosynthesis* / Q000737:chemistry / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D004285:Dogs; D005260:Female; D006325:Heart Atria; D007473:Ion Channels; D008969:Molecular Sequence Data; D009036:Moths; D009206:Myocardium / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D010053:Ovary / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D029867:Xenopus Proteins; D014982:Xenopus laevis
D000199:Actins; C498731:CLNS1A protein, Xenopus; D018118:Chloride Channels; D002712:Chlorides; C498729:Clns1a protein, rat; D007473:Ion Channels; D011994:Recombinant Proteins; D029867:Xenopus Proteins