Localization of xanthine dehydrogenase mRNA in horse skeletal muscle by in situ hybridization with digoxigenin-labelled probe.
In situ hybridization was used to localize xanthine dehydrogenase (XDH) mRNA in horse skeletal muscle. Capillary endothelial cells were found to express XDH, but muscle cells did not give any signal. The digoxigenin-labelled probe was produced by PCR with primers based on the cDNA sequence of mouse XDH and horse lung cDNAs. A 4.3 kb mRNA was detected in a Northern blot.
Räsänen
L A
LA
Department of Biochemistry, College of Veterinary Medicine, Helsinki, Finland.
Karvonen
U
U
Pösö
A R
AR
D000818:Animals; D016375:Antisense Elements (Genetics); D001483:Base Sequence; D015152:Blotting, Northern; D002196:Capillaries; D004247:DNA; D004076:Digoxigenin; D004730:Endothelium, Vascular / Q000201:enzymology; D006736:Horses; D017403:In Situ Hybridization / Q000379:methods; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000201:enzymology*; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D014968:Xanthine Dehydrogenase / Q000096:biosynthesis*
D016375:Antisense Elements (Genetics); D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D004247:DNA; D014968:Xanthine Dehydrogenase; D004076:Digoxigenin