Plasminogen activation activity of urokinase determined via a cascade enzymatic reaction system.
A method for determining the plasminogen activation rate by urokinase via a cascade enzymatic reaction system is presented. A procedure of parameter estimation has been proposed for the determination of the activity of urokinase and the kinetic constants. Urokinase from urine has been successfully assayed through use of a plasminogen concentration lower than its saturating level. The methodology presented in this work may be adopted for the analysis of other cascade enzymatic reaction systems.
Lee
W C
WC
Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taiwan.
Chuang
C Y
CY
Chang
R M
RM
D006801:Humans; D007700:Kinetics; D008433:Mathematics; D010958:Plasminogen / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism* / Q000652:urine
D010958:Plasminogen; D014568:Urokinase-Type Plasminogen Activator