IFN-gamma is directly cytostatic to the extracellular form (promastigote) of Leishmania donovani.
We investigated the effect of various human cytokines on the in vitro growth of Leishmania donovani (L. donovani) promastigotes. rhIFN-alpha, rhIFN-beta, rhIL-1, rhIL-2, rhIL-3, rhIL-4, rhIL-6 and rhM-CSF had no effect. By contrast, rhIFN-gamma was directly growth inhibitory to L. donovani promastigotes in a dose-dependent manner. Anti-IFN-gamma antibody, but neither anti-IFN-alpha nor anti-IFN-beta antibody, abolished this promastigote growth inhibitory effect of rhIFN-gamma. L. donovani promastigotes were not lysed by rhIFN-gamma as determined by 51Cr-release assay. These data indicate that rhIFN-gamma is cytostatic, not cytotoxic, to L. donovani promastigotes.
Bhattacharya
S
S
Indian Institute of Chemical Biology, Calcutta.
Manna
P P
PP
Bandyopadhyay
S
S
D000818:Animals; D002860:Chromium Radioisotopes; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007893:Leishmania donovani / Q000187:drug effects* / Q000254:growth & development*; D008262:Macrophage Activation / Q000187:drug effects; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology
D002860:Chromium Radioisotopes; D007370:Interferon Type I; D007375:Interleukin-1; D007376:Interleukin-2; D007377:Interleukin-3; D015850:Interleukin-6; D011994:Recombinant Proteins; D015847:Interleukin-4; D016899:Interferon-beta; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma