title: Nucleotide sequence of levansucrase gene (levU) of Zymomonas mobilis ZM1 (ATCC10988).
abstract: The extracellular levansucrase gene (levU) was cloned from the genomic DNA of Zymomonas mobilis ZM1 and the nucleotide sequence of the levU structural gene was determined. The 3.1 kb EcoRV-polylinker fusion DNA fragment containing the levU gene had an open reading frame of 1272 bps and the deduced amino acid sequence was 423 residues long with a molecular weight of 46,725. Although this protein exhibited little similarity with other known levansucrases, several well-conserved regions were observed (1-5 regions).
authors.affiliation: Metabolic Engineering Laboratory, Genetic Engineering Research Institute, KIST, Taejon, South Korea.
authors.forename: K B
authors.initials: KB
authors.lastname: Song
authors.forename: H K
authors.initials: HK
authors.lastname: Joo
authors.forename: S K
authors.initials: SK
authors.lastname: Rhee
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D005798:Genes, Bacterial; D006602:Hexosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D017355:Zymomonas / Q000201:enzymology / Q000235:genetics*
chemical_list: D006602:Hexosyltransferases; C021513:levansucrase