title: The humoral and cell mediated immune response of young chicks to Salmonella typhimurium and S. Kedougou.
abstract: Day old chicks were inoculated with either Salmonella typhimurium or S. Kedougou as representative examples of an invasive and a non-invasive strain respectively. The titres of IgA, IgG and IgM antibodies were determined, using an enzyme-linked immunosorbent assay (ELISA) in the bile, serum and homogenates of intestinal mucosa each week up to 5 weeks of age. Statistically significant increases in antibodies were detected in the bile (IgA and IgG) and the intestinal mucosa and serum (IgG and IgM) 1 week after the birds were inoculated with S. typhimurium. In contrast, only a limited response was recorded for S. kedougou 4 weeks after challenge commenced. The birds appeared capable of eliminating systemic infection with S. typhimurium as they grew older although a cell mediated immune response was demonstrated in less than half the birds aged 4 or 5 weeks. The demonstration of only a limited serological response to S. kedougou indicates that serological testing may have a limited role in monitoring flocks for infection with non-invasive serotypes.
authors.affiliation: Department of Veterinary Medicine, University of Bristol, Langford, Avon, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Brito
authors.forename: M
authors.initials: M
authors.lastname: Hinton
authors.forename: C R
authors.initials: CR
authors.lastname: Stokes
authors.forename: G R
authors.initials: GR
authors.lastname: Pearson
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D002645:Chickens / Q000276:immunology*; D007111:Immunity, Cellular; D007136:Immunoglobulins / Q000096:biosynthesis; D008213:Lymphocyte Activation; D011201:Poultry Diseases / Q000276:immunology*; D012475:Salmonella / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007136:Immunoglobulins