Lactulose and mannitol as probe markers for in vivo assessment of passive intestinal permeability in healthy cats.
Intestinal permeability was assessed in 12 healthy cats by use of a differential sugar absorption test. A 50-ml isotonic aqueous solution containing a combination of 1.8 g of the disaccharide lactulose and 1.7 g of the monosaccharide mannitol was administered to cats via nasogastric tube. Urine was collected after 6 hours, and all urine samples were analyzed the same day, using a gas-liquid chromatographic technique (GLC) and an enzymatic assay (ENZ). Median urinary recovery of lactulose was 0.27 and 0.54% determined by GLC and ENZ, respectively. Differences between these groups were statistically significant (P = 0.023), and correlation between assays was high (r = 0.94, P < 0.01). Median urinary recovery of mannitol was 1.93 and 2.09% for GLC and ENZ, respectively. There were no statistically significant differences between these groups and the correlation between assays was high (r = 0.85, P < 0.01). The median lactulose-to-mannitol ratio was 0.29, using GLC, and was 0.52, using ENZ. Correlation of these ratios was again high (r = 0.93, P < 0.01).
Papasouliotis
K
K
Department of Veterinary Medicine, University of Bristol, England.
Gruffydd-Jones
T J
TJ
Sparkes
A H
AH
Cripps
P J
PJ
Millard
W G
WG
D000284:Administration, Oral; D000818:Animals; D002415:Cats / Q000378:metabolism* / Q000652:urine; D002849:Chromatography, Gas / Q000662:veterinary; D005260:Female; D007275:Injections, Intravenous / Q000662:veterinary; D007408:Intestinal Absorption / Q000502:physiology*; D007792:Lactulose / Q000652:urine; D008297:Male; D008353:Mannitol / Q000652:urine
D008353:Mannitol; D007792:Lactulose