title: The effect of vanadate of arachidonic acid metabolism in human amnion cells.
abstract: This investigation was designed to investigate the effects of vanadate on arachidonic acid metabolism in human amnion cell in primary monolayer culture that served as a model system. The secretion of prostaglandin E2 (PGE2) into the culture medium was quantified by radioimmunoassay. The rate of conversion of [14C] arachidonic acid to [14C] PGE2 (PGE2 synthase) was determined in cell sonicates under optimal in vitro conditions. A maximal stimulation of PGE2 production and PGE2 synthase activity was determined with vanadate at a concentration of 32 microM) was effective maximally after 4 h of treatment, i.e., the production of PGE2 was stimulated 2,3-fold, and the specific activity of PGE2 synthase 2,1-fold compared with control incubations, respectively. We suggest that vanadate acts to increase the rate of conversion of arachidonic acid to PGE2 by a mechanism that involves de novo protein synthesis or that alters the phosphorylation state of enzymes that are requisite for the conversion of arachidonic acid to PGE2.
authors.affiliation: Department of Obstetrics and Gynecology University of Tübingen, F.R.G.
authors.forename: K
authors.initials: K
authors.lastname: Korte
authors.forename: K
authors.initials: K
authors.lastname: Marzusch
authors.forename: M L
authors.initials: ML
authors.lastname: Casey
mesh_terms: D000650:Amnion / Q000187:drug effects / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D016718:Arachidonic Acid; D014638:Vanadates; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone