Gamma irradiation increases hsp-70 in Chinese hamster ovary cells.
The induction of hsp-70 by gamma irradiation was studied in Chinese hamster ovary (CHO) cells. Synthesis of hsp-70 was assessed by SDS-PAGE of radiolabeled proteins while levels of hsp-70 mRNA were estimated by Northern blot analysis of total RNA. SDS-PAGE analysis indicated that the synthesis of hsp-70 was enhanced in cells exposed to 400 or 1000 Gy. Northern blot analysis of RNA from cells exposed to 1000 Gy indicated a rapid increase in mRNA capable of hybridizing to an hsp-70 oligonucleotide probe. These experiments represent the first report demonstrating that ionizing radiation can increase hsp-70 mRNA.
Sierra-Rivera
E
E
Vanderbilt Center for Radiation Oncology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Voorhees
G J
GJ
Freeman
M L
ML
D000818:Animals; D015152:Blotting, Northern; D016466:CHO Cells / Q000378:metabolism / Q000528:radiation effects*; D006224:Cricetinae; D005720:Gamma Rays; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism
D006360:Heat-Shock Proteins; D012333:RNA, Messenger