title: Detection and quantification of 8-hydroxydeoxyguanosine adducts in peripheral blood of people exposed to ionizing radiation.
abstract: Ionizing radiation produces a variety of damaging insults to nucleic acids, including the promutagenic lesion 8-hydroxydeoxyguanosine. In the present study, the 8-hydroxydeoxyguanosine content of peripheral blood leukocyte DNA isolated from individuals exposed to therapeutic doses of ionizing radiation was determined by a HPLC-coupled 32P-postlabeling assay. Peripheral blood leukocyte DNA from individuals irradiated with 180-200 cGy were observed to contain 2-4.5 times as much 8-hydroxydeoxyguanosine as that from unexposed individuals. These results were confirmed by the use of a HPLC-coupled electrochemical detection system. Thus, human exposure to ionizing radiation significantly increased the circulating leukocyte DNA content of 8-hydroxydeoxyguanosine.
authors.affiliation: Department of Pathology, University of Colorado Health Sciences Center, Denver 80218-1088.
authors.forename: V L
authors.initials: VL
authors.lastname: Wilson
authors.forename: B G
authors.initials: BG
authors.lastname: Taffe
authors.forename: P G
authors.initials: PG
authors.lastname: Shields
authors.forename: A C
authors.initials: AC
authors.lastname: Povey
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000097:blood / Q000528:radiation effects*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D010761:Phosphorus Radioisotopes; D011879:Radiotherapy Dosage
chemical_list: D010761:Phosphorus Radioisotopes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine