Annexin 1 is present in different molecular forms in rat cerebral cortex.
Previously we have purified annexin 1 [J. Neurochem. 56 (1991) 1985-1986] from pig cerebral cortex as a monomeric protein of 37 kDa. Here, the localization of annexin 1 was investigated in subcellular fractionations of rat cerebral cortex using immunodetection by a specific antibody. In contrast to synaptophysin, a specific synaptic vesicle integral membrane protein, annexin 1 is located in the synaptic plasma membrane fraction where it appears on SDS-PAGE as a polypeptide of 74 kDa. Annexin 1 is extracted also as a 74 kDa polypeptide from the purified synaptic plasma membranes. These results suggest for the 74 kDa molecular form an enzymatic dimerization of annexin 1 when associated to the membrane.
Pradel
L A
LA
Unité CNRS UA 1112, Neurobiologie Physico-Chimique, Paris, France.
Rendon
A
A
D000818:Animals; D017305:Annexin A1 / Q000032:analysis* / Q000302:isolation & purification; D002540:Cerebral Cortex / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D051381:Rats; D013347:Subcellular Fractions; D013570:Synaptic Membranes / Q000737:chemistry; D016708:Synaptophysin / Q000032:analysis
D017305:Annexin A1; D016708:Synaptophysin