Isolation of multiple activities from mouse FM3A cells which promote homologous pairing of DNA molecules.
We describe the detection and partial purification of three homologous pairing activities from extracts of mouse mammary carcinoma FM3A cells. These activities, designated MHP1a, 1b, and 1c, form joint molecules between circular single-stranded DNA and homologous linear duplex DNA and are distinguished from one another by their chromatographic behaviors or isoelectric properties. The reactions promoted by these activities require homologous substrates but not ATP. All the reactions also show Mg2+ dependence in the absence of exogenous ATP. Analysis of the reaction products has revealed that strand exchange proceeds for lengths of up to at least 271 bp during the homologous pairing reaction. The finding of multiple types of homologous pairing and strand exchange activity in mouse cells may facilitate the elucidation of the mechanism of homologous recombination in somatic mammalian cells.
Shimokawa
T
T
Kihara Institute for Biological Research, Yokohama City University, Japan.
Mishina
Y
Y
Koyama
H
H
D000645:Ammonium Sulfate; D000818:Animals; D001483:Base Sequence; D002457:Cell Extracts / Q000737:chemistry*; D002458:Cell Fractionation; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded; D008274:Magnesium; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured
D002457:Cell Extracts; D004277:DNA, Single-Stranded; D011506:Proteins; D004247:DNA; D008274:Magnesium; D000645:Ammonium Sulfate