The superoxide scavenging activity of dihydroquinoline type derivatives (CH402 and MTDQ-DA).
Electron paramagnetic resonance/spin trapping studies were applied, to verify the superoxide radical scavenging activity of two non-toxic, water soluble dihydroquinoline type antioxidants, CH402 (Na-2,2-dimethyl-1,2-dihydroquinoline-4-yl methane sulphonate and MTDQ-DA (6,6-methylene bis 2,2-dimethyl-4-methane sulphonic acid: Na-1,2-dihydroquinoline). Results were compared with other indirect methods such as the amperometric, spectrophotometric and luminometric methods, respectively. Both dihydroquinoline type antioxidants scavenged superoxide in vitro specifically. MTDQ-DA scavenged superoxide an order of magnitude faster than CH-402. Neither CH402 nor MTDQ-DA affected the hypoxanthine/xanthine oxidase superoxide generating system, nor did they inhibit xanthine oxidase directly.
Prònai
L
L
2nd Department of Medicine, Semmelweis University Medical School, Budapest, Hungary.
Blåzovics
A
A
Horvåth
M
M
Lång
I
I
Fehér
J
J
D000975:Antioxidants / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D016166:Free Radical Scavengers; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000737:chemistry; D008163:Luminescent Measurements; D010100:Oxygen / Q000032:analysis; D011804:Quinolines / Q000737:chemistry*; D013053:Spectrophotometry; D013481:Superoxides / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000737:chemistry
D000975:Antioxidants; D016166:Free Radical Scavengers; D007042:Hypoxanthines; D011804:Quinolines; D013481:Superoxides; D019271:Hypoxanthine; C059107:CH 402; C044964:6,6'-methylenebis(2,2-dimethyl-4-methanesulfonic acid-1,2-dihydroquinoline); D014969:Xanthine Oxidase; D010100:Oxygen