title: Characterization of NIGMS human/rodent somatic cell hybrid mapping panel 2 by PCR.
abstract: NIGMS human somatic cell hybrid mapping panel 2 was tested for specific markers for each human chromosome. Several discrepancies with the reported chromosome content were found by screening with PCR primers. Our results indicated that there were additional chromosomal fragments present in some of the cell lines and that a few of the hybrids had lost part of the reported chromosome.
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
authors.forename: B L
authors.initials: BL
authors.lastname: Dubois
authors.forename: S L
authors.initials: SL
authors.lastname: Naylor
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D009316:National Institutes of Health (U.S.); D016133:Polymerase Chain Reaction; D012015:Reference Standards; D014481:United States
chemical_list: D005819:Genetic Markers