Selective isolation of highly polymorphic (dC-dA)n.(dG-dT)n microsatellites by stringent hybridization.
We defined a hybridization condition for isolation of clones carrying long (dC-dA)n.(dG-dT)n microsatellites from a genomic plasmid library. By using (dC-dA)20 oligonucleotide as a probe and hybridizing under stringent conditions, more than two-thirds of the obtained plasmid carried the repeats that were longer than (dC-dA)14 and highly polymorphic.
Iizuka
M
M
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
Makino
R
R
Sekiya
T
T
Hayashi
K
K
D002880:Chromosomes, Human, Pair 11; D004276:DNA, Satellite / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D015345:Oligonucleotide Probes; D011089:Polydeoxyribonucleotides / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic
D004276:DNA, Satellite; D015345:Oligonucleotide Probes; D011089:Polydeoxyribonucleotides; C033927:poly(dA-dC).poly(dG-dT)