[Effects of lung- and liver-derived biomatrices on the attachment and growth of tumor cells].
The attachment and growth property of a human carcinoma cell line (Anip), which has high pulmonary metastatic potential, were investigated in vitro on the lung- or liver-derived biomatrix in culture medium supplemented with serum (SSM) or free of serum (SFM). Either in SFM or SSM, the carcinoma cells showed a higher rate of adhesion to the lung biomatrix than that to the liver biomatrix, In SFM, cells plated on plastic plates ceased proliferating and died; whereas those on the biomatrices, particularly the cells on the lung biomatrix survived and proliferated. Moreover, in SSM, carcinoma cells on the lung biomatrix had the highest clonal growth efficiency. These results indicate that biomatrices may provide conditions favourable for cell growth, and may compensate in part the deficiency of nutrition and growth factors when serum is absent in the culture medium. The different influences exerted on the growth and attachment rate of tumor cells by biomatrices derived from different organs also help to clarify the mechanism of organ specificity of tumor metastasis.
Wang
M L
ML
Department of Pathology, Harbin Medical University.
D000230:Adenocarcinoma / Q000473:pathology*; D002448:Cell Adhesion; D002455:Cell Division; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D008099:Liver / Q000166:cytology; D008168:Lung / Q000166:cytology; D008175:Lung Neoplasms / Q000473:pathology*; D009928:Organ Specificity; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay