Cytogenetic findings in eleven gastric carcinomas.
We describe the results of the cytogenetic study of 10 primary adenocarcinomas of the stomach and one lymph node metastasis of a gastric adenocarcinoma after direct harvesting or short-term in vitro culture. All cases showed a variable number of numerical and/or structural clonal cytogenetic aberrations. Polysomy of chromosomes 2 and 20 were the most common numerical abnormalities. Rearrangements of chromosomes 1, 3, 7, and 13 were each observed in more than half the cases. Chromosomes 3 and 13 were the chromosomes more often exhibiting structural cytogenetic aberrations. In five tumors, rearrangements of chromosome 6 resulting in partial deletion of 6q were noted (common deleted region 6q21-22-->qter). The recurrent markers observed in our series were an i(8q) and an i(17q) in three and two cases, respectively. Double minutes (dmin) or homogeneously staining regions (hsr) were evident in three tumors. Contrary to the recent claim that structural abnormalities affecting 11p13-p15 were specifically involved in gastric cancer, we detected rearrangements of this region in only two cases.
Seruca
R
R
Unit of Genetics, Institute of Molecular Pathology and Immunology (IPATIMUP), Porto, Portugal.
Castedo
S
S
Correia
C
C
Gomes
P
P
Carneiro
F
F
Soares
P
P
de Jong
B
B
Sobrinho-Simões
M
M
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics*