title: beta'-COP, a novel subunit of coatomer.
abstract: Several lines of evidence favour the hypothesis that intracellular biosynthetic protein transport in eukaryotes is mediated by non-clathrin-coated vesicles (for a review see Rothman and Orci, 1992). The vesicles have been isolated and a set of their surface proteins has been characterized as coat proteins (COPs). These COPs exist in the cytosol as a preformed complex, the coatomer, which was prior to this study known to contain six subunits: four (alpha-, beta-, gamma- and delta-COP) with molecular weights between 160 and 58 kDa, and two additional proteins of approximately 36 and 20 kDa, epsilon- and xi-COP. Here we describe a novel subunit of the coatomer complex, beta'-COP. This subunit occurs in amounts stoichiometric to the established COPs both in the coatomer and in nonclathrin-coated vesicles and shows homology to the beta-subunits of trimeric G proteins.
authors.affiliation: Institute of Biochemistry I, University of Heidelberg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Stenbeck
authors.forename: C
authors.initials: C
authors.lastname: Harter
authors.forename: A
authors.initials: A
authors.lastname: Brecht
authors.forename: D
authors.initials: D
authors.lastname: Herrmann
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: L
authors.initials: L
authors.lastname: Orci
authors.forename: F T
authors.initials: FT
authors.lastname: Wieland
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002213:Capsid / Q000737:chemistry*; D002417:Cattle; D020756:Coatomer Protein; D006224:Cricetinae; D004247:DNA; D006056:Golgi Apparatus / Q000737:chemistry / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000737:chemistry / Q000648:ultrastructure; D008565:Membrane Proteins / Q000737:chemistry*; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D011817:Rabbits
chemical_list: D020756:Coatomer Protein; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D004247:DNA