Effects of ionic strength and pH on the dissociation constant between NADPH-adrenoferredoxin reductase and adrenoferredoxin.
The dissociation constant between NADPH-adrenoferredoxin reductase and adrenoferredoxin was measured at various ionic strengths (0.05-0.25) and pHs (6.6-8.2) by means of a kinetic method involving pyridoxal 5'-phosphate. For each ionic strength, the maximum dissociation constant was obtained at pH 7.4. It ranged from 8.34 (ionic strength = 0.05) to 169 nM (ionic strength = 0.25) at pH = 7.4. At each pH, the dissociation constant increased along with the increase in ionic strength.
Hamamoto
I
I
First Department of Surgery, Kagawa Medical School, Japan.
Kurokohchi
K
K
Tanaka
S
S
Ichikawa
Y
Y
D000325:Adrenodoxin / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009994:Osmolar Concentration; D011732:Pyridoxal Phosphate / Q000378:metabolism
D000325:Adrenodoxin; D011732:Pyridoxal Phosphate; D005287:Ferredoxin-NADP Reductase; D009251:NADPH-Ferrihemoprotein Reductase