Copper deficiency increases the susceptibility of lipoproteins and tissues to peroxidation in rats.
Male Wistar rats were pair-fed from weaning for 6 wk either a copper-deficient diet or a control diet containing sucrose. Hypercholesterolemia in copper-deficient animals was associated with a significantly greater plasma apolipoprotein B concentration and modifications in the lipid composition of triglyceride-rich lipoproteins containing apolipoprotein B. The proportion of triglycerides was elevated and the proportion of cholesterol was reduced in the VLDL + LDL fractions from copper-deficient rats compared with control animals. When the VLDL + LDL fractions were subjected to in vitro copper-induced oxidation, the lipoprotein fractions from copper-deficient rats were more susceptible to oxidative damage than lipoprotein fractions from control rats as indicated by the rate of diene conjugation. Hydroperoxide and thiobarbituric acid-reactive substance (TBARS) measurements performed 3 h after the induction of peroxidation were in agreement with the greater formation of conjugated dienes. Concentrations of TBARS were significantly greater in heart homogenates from copper-deficient rats compared with control rats. After exposure of tissue homogenates to iron-induced lipid peroxidation, TBARS were significantly higher in liver and heart from copper-deficient rats compared with control rats. Of particular importance is the observation that copper deficiency has a harmful effect on lipid peroxidation in the cardiovascular system.
Rayssiguier
Y
Y
Laboratoire des Maladies Métaboliques, Institut National de la Recherche Agronomique, St-Genès-Champanelle, France.
Gueux
E
E
Bussiere
L
L
Mazur
A
A
D000818:Animals; D001055:Apolipoproteins B / Q000097:blood; D002784:Cholesterol / Q000032:analysis / Q000097:blood; D003300:Copper / Q000097:blood / Q000172:deficiency*; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000032:analysis; D008074:Lipoproteins / Q000737:chemistry / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013395:Sucrose / Q000008:administration & dosage; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014280:Triglycerides / Q000032:analysis / Q000097:blood
D001055:Apolipoproteins B; D004040:Dietary Carbohydrates; D008054:Lipid Peroxides; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D013395:Sucrose; D003300:Copper; D002784:Cholesterol