title: Stimulation of the bactericidal activity of polymorphonuclear leukocytes by manganese.
abstract: An antioxidant effect of manganese (Mn) complexes due to the scavenging of the superoxide anion (O2-.) or to the decomposition of hydrogen peroxide (H2O2) has been described. We report here that Mn also can exert a prooxidant effect under certain experimental conditions. Thus Mn2+ in phosphate buffer increased the bactericidal effect of phorbol myristate acetate-stimulated polymorphonuclear leukocytes (PMNs) on extracellular Escherichia coli. This effect was inhibited by azide, catalase, and a decrease in chloride concentration and was not observed when normal PMNs were replaced by those of patients with chronic granulomatous disease or myeloperoxidase (MPO) deficiency. Mn2+ could be replaced by Mn3+ or by superoxide dismutase (SOD). These findings suggest that Mn (or SOD), by increasing the conversion of O2-. to H2O2, can increase the activity of the MPO-H2O2-chloride antimicrobial system released by stimulated PMNs.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195.
authors.forename: S J
authors.initials: SJ
authors.lastname: Klebanoff
authors.forename: K G
authors.initials: KG
authors.lastname: Schlechte
authors.forename: A M
authors.initials: AM
authors.lastname: Waltersdorph
mesh_terms: D001770:Blood Bactericidal Activity / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D013482:Superoxide Dismutase / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D008345:Manganese; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate