[Analysis of dextromethorphan and its metabolites in human urine by using gas chromatography-mass spectrometry].
A GC-MS method for the analysis of dextromethorphan and its metabolites is described. The urine sample was hydrolyzed with HCl, extracted with diethyl ether and derivatized with MSTFA (N-methyl-N-trimethylsilyltrifluoroacetamide)-MBTFA (N-methyl-bistrifluoroacetamide). Dextromethorphan and its 3 metabolites were detected in urine samples within 2-60 h after administration of the drug. Their structures and the variation of their concentration in urine were determined. The detection limit of dextromethorphan is 10 ng.
Xu
Y X
YX
China Doping Control Center, National Research Institute of Sports Medicine, Beijing.
Shen
L
L
Zhang
C J
CJ
D003915:Dextromethorphan / Q000378:metabolism / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007981:Levorphanol / Q000652:urine
D007981:Levorphanol; D003915:Dextromethorphan