title: Evidence for selective release of rodent islet amyloid polypeptide through the constitutive secretory pathway.
abstract: To determine the potential for differential release of islet amyloid polypeptide and insulin, we performed studies in rat islet monolayer cultures under conditions known to impair regulated beta-cell secretion. In inhibiting concentrations of epinephrine or the absence of calcium, islet amyloid polypeptide was secreted through a constitutive pathway while insulin was not. These findings suggest a mechanism for persistent islet amyloid polypeptide secretion and amyloid accumulation when regulated insulin release is impaired as in Type 2 (non-insulin-dependent) diabetes mellitus and insulinomas.
authors.affiliation: Department of Medicine, University of Washington, Seattle.
authors.forename: S E
authors.initials: SE
authors.lastname: Kahn
authors.forename: C B
authors.initials: CB
authors.lastname: Verchere
authors.forename: D A
authors.initials: DA
authors.lastname: D'Alessio
authors.forename: D L
authors.initials: DL
authors.lastname: Cook
authors.forename: W Y
authors.initials: WY
authors.lastname: Fujimoto
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000682:Amyloid; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D005947:Glucose; D002118:Calcium; D004837:Epinephrine