The 3'-terminal untranslated region of alfalfa mosaic virus RNA 4 facilitates the RNA entry into translation in a cell-free system.
In order to understand the role of the 3'-terminal untranslated region (3'-UTR) of alfalfa mosaic virus (AlMV) RNA 4 in viral RNA translation we have constructed the RNA derivatives differing in the length of their 3'-terminal portions and expressed them in a wheat germ extract. The result shows that the removal of the 3'-UTR from AlMV RNA 4 causes a lagged RNA translation in the cell-free system as compared with the translation of the full length RNA 4, thus suggesting the involvement of the 3'-UTR in the translation initiation pathway.
Ryabova
L A
LA
Institute of Protein Research, Russian Academy of Sciences, Moscow Region.
Torgashov
A F
AF
Kurnasov
O V
OV
Bubunenko
M G
MG
Spirin
A S
AS
D002474:Cell-Free System; D007700:Kinetics; D009029:Mosaic Viruses / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014908:Triticum
D012333:RNA, Messenger; D012367:RNA, Viral