Follicular fluid administration delayed, but did not prevent, the hemicastration-induced increase in follicle-stimulating hormone secretion and compensatory testicular hypertrophy in ram lambs.
To determine whether the post-hemicastration rise in serum FSH is related to compensatory testicular hypertrophy, 4-mo-old ram lambs were hemicastrated and injected s.c. with 5 ml of saline (HC; n = 5) or steroid-free follicular fluid (HC + FF; n = 5) twice daily for 28 days. Sham-operated intact lambs, injected with saline, served as controls (INT; n = 5). Blood samples were collected frequently during the first 48 h, daily for the remainder of the first week, and then three times/wk for the next 11 wk. Serum FSH concentrations in HC lambs increased above presurgery levels by 9 h, peaked at 13 days, and returned to baseline as compared to INT controls by 5 wk. Follicular fluid administration delayed (p < 0.05) the hemicastration-induced rise in FSH, but only for 14 of the 28 days of treatment; after this time, concentrations increased to the levels observed in HC rams. Within 2 days after the last follicular fluid injection (Day 30), FSH concentrations increased again to levels greater (p < 0.05) than those observed in HC animals. FSH secretion in HC + FF rams then declined to basal values by 7 wk, 2 wk later than in HC rams. Although the time course differed, the duration of elevated FSH did not differ between HC and HC + FF rams (36.4 +/- 2.0 vs. 37.8 +/- 4.7 days, respectively; p > 0.05). Testicular volume, determined on the last day of treatment (Day 28), was 70% greater (p < 0.05) in HC than in INT or HC + FF lambs; volume did not differ (p > 0.05) between INT and HC + FF rams.(ABSTRACT TRUNCATED AT 250 WORDS)
Brown
J L
JL
Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences Bethesda, Maryland 20814.
Schoenemann
H M
HM
Chakraborty
P K
PK
D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000378:metabolism*; D015571:Follicular Fluid / Q000502:physiology*; D006984:Hypertrophy; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009919:Orchiectomy; D009929:Organ Size; D012756:Sheep; D013737:Testis / Q000473:pathology*; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone