Spontaneous development of anti-collagen type II antibodies with NTA, and anti-DNA antibodies in senescence-accelerated mice.
We previously reported that circulating natural thymocytotoxic autoantibody (NTA) and IgG-anti-DNA antibodies were the major serological characteristics of a substrain of SAM, SAM-P/1. We present here a study of ageing in which we further measured and compared various kinds of circulating IgG antibodies including anti-collagen type II, rheumatoid factor (RF), and anti-2,4,-dinitrophenol (DNP), between SAM-P/1 and control SAM-R/1 mice. The results showed that age-associated increases in anti-collagen type II antibodies in SAM-P/1 were distinctively higher than those in SAM-R/1 when the mice were over 4 months of age, and the increases were significantly correlated with increases in NTA, anti-DNA antibodies, RF activities and anti-DNP antibodies. Anti-collagen type II antibody activity was not significantly inhibited by preincubating the antibodies with DNA, IgG-Fc and DNP-BSA samples. These findings suggest that antibodies specifically directed against collagen type II can be produced in a background of polyclonal B cell activation, and that these antibodies in association with NTA and anti-DNA antibodies may play a pathogenic role in the development of accelerated senescence in SAM-P/1 mice.
Yoshioka
H
H
Department of Geriatric Medicine, Kyoto University Hospital, Japan.
Yoshida
H
H
Usui
T
T
Sung
M
M
Ko
K
K
Takeuchi
E
E
Kita
T
T
Sugiyama
T
T
D000375:Aging / Q000276:immunology*; D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood*; D001323:Autoantibodies / Q000096:biosynthesis / Q000097:blood*; D003094:Collagen / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D013601:T-Lymphocytes / Q000276:immunology*
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D007074:Immunoglobulin G; D003094:Collagen; D004247:DNA