title: Effect of chronic portal hypertension on glutamine transport across rat intestinal brush border and basolateral membranes.
abstract: The present study examined the effect of chronic portal hypertension on the intestinal transport of glutamine across the brush border membrane (BBM) and the basolateral membrane (BLM) of the rat enterocyte. Chronic portal hypertension was induced by partial portal vein ligation, and the results were compared with results in sham-operated rats. Transport studies were performed by using established techniques of isolated membrane vesicles. In chronic portal hypertensive rats, transport of glutamine in BBM vesicles (BBMVs) exhibited significant inhibition in the maximal velocity (Vmax) of both the Na(+)-dependent and the Na(+)-independent transport processes, with no significant changes in the apparent Michaelis-Menten constant (Km) of the carrier systems. Similarly, chronic portal hypertension caused a significant decrease in the Vmax of the Na(+)-dependent and the Na(+)-independent uptake processes in intestinal BLM vesicles (BLMVs), with no change in the apparent Km of the uptake systems. These changes in glutamine transport in BBMVs and BLMVs brought about by chronic portal hypertension are not due to differences in the relative purity of the vesicular preparations between the portal hypertensive and sham-operated rats, because similar degrees of enrichment and depurification of marker enzyme activities were seen in final vesicular preparations in the two rat groups. These results demonstrate that in chronic portal hypertension, glutamine transport across both poles of the enterocyte is impaired. At both the BBM and BLM domains, the impairment appears to be mediated via a decrease in the number (and/or activity) of the glutamine carrier systems, with no changes in their affinity.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Medical Research Service, VA Medical Center, Long Beach, CA 90822.
authors.forename: H M
authors.initials: HM
authors.lastname: Said
authors.forename: T
authors.initials: T
authors.lastname: Morgan
authors.forename: J
authors.initials: J
authors.lastname: Hoefs
mesh_terms: D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D005973:Glutamine / Q000378:metabolism*; D006975:Hypertension, Portal / Q000378:metabolism*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005973:Glutamine