Sequencing of a urinary stone protein, identical to alpha-one antitrypsin, which lacks 22 amino acids.
We have extracted and purified proteins from calcium containing urinary stones with 4 M guanidine chloride and sequenced the amino acids of the proteins. The protein of approximately 49kDa was defined as human alpha-one antitrypsin (AT, from 23rd to 42nd amino acids of NH2-terminal) by amino acid sequence analysis. The ratio of the amount of AT to total proteins was 34.9% analyzed by densitometer. AT is presumably involved in stone formation as a coprecipitating substance because equilibrium dialysis revealed that AT has no affinity for calcium.
Umekawa
T
T
Department of Urology, School of Medicine, Kinki University, Osaka, Japan.
Kohri
K
K
Amasaki
N
N
Yamate
T
T
Yoshida
K
K
Yamamoto
K
K
Suzuki
Y
Y
Sinohara
H
H
Kurita
T
T
D000595:Amino Acid Sequence; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D014545:Urinary Calculi / Q000737:chemistry*; D000515:alpha 1-Antitrypsin / Q000737:chemistry*
D011506:Proteins; D000515:alpha 1-Antitrypsin