title: Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.
abstract: A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.
authors.affiliation: Department of Pathology, Indiana University School of Medicine, Indianapolis 46202.
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: N L
authors.initials: NL
authors.lastname: Bauer
authors.forename: M M
authors.initials: MM
authors.lastname: Shaw
authors.forename: M M
authors.initials: MM
authors.lastname: Durkin
authors.forename: M S
authors.initials: MS
authors.lastname: Bartlett
authors.forename: S F
authors.initials: SF
authors.lastname: Queener
authors.forename: J W
authors.initials: JW
authors.lastname: Smith
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004271:DNA, Fungal / Q000235:genetics; D004622:Embryo, Mammalian; D005069:Evaluation Studies as Topic; D005260:Female; D005347:Fibroblasts; D006801:Humans; D008168:Lung; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods*; D011010:Pneumocystis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004271:DNA, Fungal