Long-term myelopoietic cultures from the renal hematopoietic tissue of the rainbow trout, Oncorhynchus mykiss W.: phenotypic characterization of the stromal cells.
This study describes the conditions for the long-term culture of the renal hematopoietic tissue of the rainbow trout, Oncorhynchus mykiss, the characterization of the stromal cells, and their relationships with myelopoietic cells. The long-term cultures consisted of stromal cells, which supported active myelopoiesis. Stromal cells were analyzed by enzyme-cytochemical techniques and electron microscopy. Major stromal cell types in long-term cultures consisted of fibroblastic reticular cells and epithelioid cells. Myelopoietic cells differentiated in close association with the fibroblastic reticular cells, and mature granulocytes were released into the culture medium.
Diago
M L
ML
Departamento de Biología Celular y Anatomía, Facultad de Biología, Universidad de León, Spain.
López-Fierro
M P
MP
Razquin
B
B
Villena
A
A
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D004848:Epithelium / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure; D006410:Hematopoiesis / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007668:Kidney / Q000166:cytology*; D008264:Macrophages / Q000737:chemistry; D008543:Melanins / Q000032:analysis; D008854:Microscopy, Electron; D010587:Phagocytosis / Q000502:physiology; D010641:Phenotype; D012474:Salmon / Q000033:anatomy & histology*; D013997:Time Factors
D008543:Melanins