A rapid method for microbial sample preparation for the scanning electron microscope.
A synthetic aromatic polymer has been used for preparing replicas of different microorganisms. This method of preparing highly concentrated (9.6 k) microbiological samples for scanning electron microscopy was compared with a standard method. The micrographs of the replicated samples are satisfactory. This method is rapid, cost effective and produces good results, especially in the case of spore-forming mycelial microorganisms.
Chaphalkar
S
S
Department of Microbiology, Maharashtra Association for the Cultivation of Science, Research Institute, Pune, India.
Dongre
R
R
Joshi
D
D
Dey
S
S
D001232:Aspergillus fumigatus / Q000648:ultrastructure; D001412:Bacillus subtilis / Q000648:ultrastructure; D001419:Bacteria / Q000648:ultrastructure*; D002175:Candida / Q000648:ultrastructure; D005658:Fungi / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D011137:Polystyrenes; D013302:Streptomyces / Q000648:ultrastructure
D011137:Polystyrenes