Fat in bone xenografts: importance of the purification procedures on cleanliness, wettability and biocompatibility.
Xenografting is a promising alternative to allografts and autografts. The remaining lipids in bone are known to influence the biocompatibility. A comparative study of wettability was done on standardized blocks of two biomaterials. A highly purified and defatted bovine bone graft (T650) was found to retain more water (2.06 g/block) than a less defatted biomaterial (T360, 0.3 g/block). Wettability, observed in the laboratory, may reflect an important in vivo property: the rapidity for extracellular fluids and blood cells to invade the graft and carry bone forming cells. When implanted in rabbit cancellous bone, T650 appears to be osteoconductive in a manner that allows trabecular architecture to be restored within 6 months.
Chappard
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D
Laboratoire d'Histologie-Embryologie, Faculté de Médecine, Angers, France.
Fressonnet
C
C
Genty
C
C
Baslé
M F
MF
Rebel
A
A
D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis / Q000302:isolation & purification*; D055592:Biophysical Phenomena; D001703:Biophysics; D016025:Bone Transplantation / Q000379:methods*; D001842:Bone and Bones / Q000737:chemistry* / Q000166:cytology; D002417:Cattle; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification*; D011817:Rabbits; D014183:Transplantation, Heterologous; D017155:Wettability
D001672:Biocompatible Materials; D008055:Lipids; C093901:Lubboc