title: Differential expression of MHC class II antigen in the contused rat spinal cord.
abstract: Following contusion injury to the dorsal surface of thoracic rat spinal cord, major histocompatibility complex (MHC) class II (Ia) antigen expression by microglia was evaluated throughout the developing lesion. Past investigations of various central nervous system (CNS) lesions have examined short-term or acute sequelae of post-traumatic Ia expression. This report demonstrates that in animals allowed to recover for 18 (sub-chronic) and 45 (chronic) days post-injury, MHC class II antigen is expressed differently at rostral and caudal extents of the lesion as compared with the lesion's epicenter. Following contusion injury to the thoracic spinal cord, sub-chronically injured animals demonstrated Ia-positive microglial staining throughout the white matter rostral and caudal to the epicenter of the lesion, whereas Ia-positive microglia and/or perivascular cells are localized within the gray matter adjacent to it. MHC class II immunoreactivity is down-regulated on microglia at chronic survival times but clusters of Ia-positive macrophages are prominent in regions of maximal degeneration at the epicenter of the lesion. Our findings support the theory that two distinct populations of macrophages participate in resolving traumatic injury. One population is the parenchymal CNS microglia and the other is presumably exudate macrophages derived from the blood. Furthermore, the immunocompetence of these cells as measured by MHC expression may be differentially regulated. This hypothesis is based on differences in Ia-positive staining observed between microglia and macrophages over time concomitant with differences in the spatial distribution of these cell types.
authors.affiliation: Department of Physiology, Ohio State University, College of Medicine, Columbus.
authors.forename: P G
authors.initials: PG
authors.lastname: Popovich
authors.forename: W J
authors.initials: WJ
authors.lastname: Streit
authors.forename: B T
authors.initials: BT
authors.lastname: Stokes
mesh_terms: D000818:Animals; D003288:Contusions / Q000276:immunology* / Q000473:pathology; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000502:physiology*; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D009457:Neuroglia / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000949:Histocompatibility Antigens Class II; D007202:Indicators and Reagents