Heterogeneity of NGF-induced differentiation in PC12 cells investigated in a battery of isolated cell clones.
The effect of NGF on a set of 17 PC12 cell clones was investigated in order to clarify some aspects concerning the changes in cell morphology and Ca2+ homeostasis that occur in the PC12 cell line after NGF-induced differentiation. The morphological parameters analyzed showed a high degree of heterogeneity among clones, consisting with a different expression of the high-affinity NGF receptor. The NGF effect on Ca2+ influx was investigated in a clone highly sensitive to the cholinergic agonist carbachol. Results showed an increase in Ca2+ influx through one of the carbachol-activated Ca2+ channels.
Clementi
E
E
Chair of Pharmacology, Faculty of Pharmacy, University of Reggio di Calabria, Catanzaro, Italy.
Raichman
M
M
Meldolesi
J
J
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000378:metabolism; D002454:Cell Differentiation; D002999:Clone Cells; D006706:Homeostasis; D009414:Nerve Growth Factors / Q000008:administration & dosage / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D011976:Receptors, Muscarinic / Q000187:drug effects
D009414:Nerve Growth Factors; D011976:Receptors, Muscarinic; D002217:Carbachol; D002118:Calcium