Cytoplasmic-nuclear trafficking of progesterone receptor. In vivo study of the mechanism of action of antiprogestins.
The signal responsible for the nuclear localization of the progesterone receptor has been characterized. The study of the mechanism of this nuclear localization has revealed that the receptor continuously shuttles between the nucleus and the cytoplasm. The receptor diffuses into the cytoplasm and is constantly and actively transported back into the nucleus. Preliminary evidence suggests that the same mechanism exists for estradiol and glucocorticoid receptors. Experiments designed to study the traffic of steroid hormone receptors have been applied to the determination of the molecular mechanism of action of antisteroids. Using these techniques, we have shown that two major antiprogestins, RU486 and ZK98299, act at the same point in the cell as the hormone.
Guiochon-Mantel
A
A
Unité de Recherche Inserm 135, Faculté de Médecine Paris-Sud, Le Kremlin Bicetre, France.
Delabre
K
K
Lescop
P
P
Perrot-Applanat
M
M
Milgrom
E
E
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D006067:Gonanes / Q000494:pharmacology; D015735:Mifepristone / Q000494:pharmacology; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics; D011372:Progestins / Q000037:antagonists & inhibitors*; D021382:Protein Sorting Signals / Q000378:metabolism*; D011817:Rabbits; D011980:Receptors, Progesterone / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D006067:Gonanes; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D011372:Progestins; D021382:Protein Sorting Signals; D011980:Receptors, Progesterone; D015735:Mifepristone; C053238:onapristone