Cloning and sequence analysis of an esterase gene from Pseudomonas sp. KWI-56.
The gene encoding an esterase from Pseudomonas sp. KWI-56 was cloned and sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide comprising 262 amino acids, whose molecular weight agreed well with the value obtained by SDS-PAGE. Comparison of the amino acid sequence with those of the other homologous enzymes suggested that Ser-92 and His-24l might be included in the catalytic triad.
Shimada
Y
Y
Osaka Municipal Technical Research Institute, Japan.
Nagao
T
T
Sugihara
A
A
Iizumi
T
T
Yui
T
T
Nakamura
K
K
Fukase
T
T
Tominaga
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004950:Esterases / Q000737:chemistry / Q000235:genetics*; D006639:Histidine; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011549:Pseudomonas / Q000235:genetics*; D016415:Sequence Alignment; D012694:Serine
D012694:Serine; D006639:Histidine; D004950:Esterases