In vitro transforming capacities of mouse c-jun:junD chimeric genes.
Among the murine Jun family of transcription factors, c-Jun and JunD are closely-related proteins with similar dimerization, DNA binding and transactivating properties. However, when expressed from a self-replicating retroviral RCAS vector, c-jun, but not junD, transforms chick embryo fibroblasts. We attempted to map the regions of c-jun which are important for transformation by constructing hybrids between c-jun and junD. Using common restriction sites, we prepared six different chimeric molecules. All of these c-jun:junD hybrids code for transactivators of AP1-containing promoters. An N-terminal segment of 79 amino acids of c-Jun converts JunD into a strong transforming protein, while other segments of c-Jun contribute to a lesser extent. Contrary to what has been reported with rat embryo fibroblasts, a c-Jun derivative with serines substituted by alanines in positions 63 and 73 still transforms CEFs efficiently.
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Laboratoire de Biologie Moléculaire et Cellulaire, Ecole Normale Supérieure, CNRS-UMR49, Lyon, France.
Piu
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Pfarr
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Yaniv
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Loiseau
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Castellazzi
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D000595:Amino Acid Sequence; D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D016758:Genes, jun; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000494:pharmacology; D016755:Proto-Oncogene Proteins c-jun / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000235:genetics / Q000494:pharmacology
D010446:Peptide Fragments; D016755:Proto-Oncogene Proteins c-jun; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators