Abnormal binding properties of blood monocytes in rheumatoid arthritis.
Blood monocytes from patients with RA exhibited a greater binding to monolayers of umbilical cord vein endothelium than monocytes from control subjects (mean 42% increase; p < 0.01). When control monocytes were added to TNF or IL-1 treated endothelium their adhesion was enhanced (mean 24% increase; p < 0.05), whereas the number of monocytes from RA patients binding to TNF or IL-1 treated monolayers was less than that adhering to untreated endothelial cells (mean 22% inhibition; p < 0.02). The surface expression of CD11b/CD18 on RA monocytes was increased and pretreatment of normal and RA cells with an anti-CD18 monoclonal antibody inhibited their attachment to untreated and cytokine-treated endothelial cells. Normal blood monocytes activated with LPS demonstrated an enhanced binding to untreated cultures (mean 23% increase; p < 0.05) and an inhibited attachment to cytokine-treated endothelial cells. This study suggests that blood monocytes in RA may be activated and that this property modifies the attachment of these cells to normal and "inflammatory" endothelium.
Mazure
G
G
Division of Immunology, UMDS, St Thomas' Hospital Campus, London, UK.
Jayawardene
S A
SA
Perry
J D
JD
McCarthy
D
D
Macey
M G
MG
Dumonde
D C
DC
Brown
K A
KA
D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000276:immunology / Q000473:pathology; D002448:Cell Adhesion / Q000276:immunology; D004730:Endothelium, Vascular / Q000276:immunology / Q000473:pathology; D006801:Humans; D009000:Monocytes / Q000276:immunology* / Q000473:pathology