Estrogen induces expression of c-jun and jun-B protooncogenes in specific rat uterine cells.
Expression of the protooncogene c-jun is induced in the uteri of ovariectomized rats in response to treatment with 17 beta-estradiol (E2-17 beta). E2 also specifically induces the uterine expression of jun-B-encoding mRNA. Medroxyprogesterone acetate and testosterone propionate treatment had no effect on the expression of c-jun- and jun-B-encoding mRNAs. Dexamethasone treatment, however, induced expression of c-jun mRNA, although less than that observed in response to E2. Cycloheximide treatment failed to block the E2-induced expression of c-jun and jun-B mRNAs, indicating that these were immediate early responses. E2-16 alpha, a short-acting estrogen, also induced c-jun and jun-B mRNA expression. The expression of c-Jun protein was examined by immunohistological methods and detected in all uterine cell types in response to treatment with estrogen. The Jun-B protein, however, was localized in uterine epithelial cells. The results of these experiments suggest that the cell type-specific expression of members of the jun family of protooncogenes may be an important regulatory event in the response of the uterus to estrogen.
Webb
D K
DK
Department of Anatomy and Cell Biology, University of Cincinnati College of Medicine, Ohio 45267-0521.
Moulton
B C
BC
Khan
S A
SA
D000818:Animals; D015152:Blotting, Northern; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D016758:Genes, jun / Q000235:genetics*; D007150:Immunohistochemistry; D007700:Kinetics; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D010052:Ovariectomy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; D003907:Dexamethasone; D003513:Cycloheximide; D017258:Medroxyprogesterone Acetate