Transformation and expression of a staphylococcal plasmid in Escherichia coli.
A multiple antibiotic-resistant Staphylococcus aureus, was found to possess three plasmid bands in agarose gel electrophoresis. A plasmid of approximately 4.3 kb (pMC790/2) was found to code for ampicillin and tetracycline resistance and to have one EcoRI site when transformed into S. aureus RN 4220. pMC790/2 in unmodified form was transformed into a recA- E. coli at a frequency of 1.2 x 10(4) transformants/micrograms of plasmid DNA. Plasmid (pMC790/2) replicated, maintained itself stably and expressed far better in the E. coli host than in S. aureus.
Saha
D
D
Department of Physiology, University College of Science and Technology, Calcutta, India.
Bal
M
M
D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli; D011815:R Factors; D013211:Staphylococcus aureus / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial