Enhanced mRNA content in response to amino acid starvation for a 73 kDa protein of the inner mitochondrial membrane.
Biosynthesis of several rat liver proteins is enhanced by amino acid deprivation of cultured hepatocytes or hepatoma cells. One of these proteins, MP-73, was synthesized at a rate 2- to 3-fold greater when cells were incubated for 3-9 h under conditions of amino acid deprivation versus amino acid supplementation. Immunoblotting with polyclonal antibodies prepared against MP-73 localized it to the inner mitochondrial membrane. MP-73 appears to be a hydrophobic, integral membrane protein. MP-73 antibody was used to identify a partial cDNA (NS3.2) of approximately 2 kb. A probe prepared from pNS3.2 identified a transcript in rat Fao hepatoma cells of approximately 4.4 kb that was increased in abundance by more than 20-fold following amino acid starvation of the cells.
Chiles
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TC
Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville 32610.
Laine
R O
RO
Shay
N F
NF
Handlogten
M E
ME
Nick
H S
HS
Kilberg
M S
MS
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA; D015870:Gene Expression; D015723:Gene Library; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008930:Mitochondria, Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D008565:Membrane Proteins; D012333:RNA, Messenger; C082025:mitochondrial membrane protein 73; D004247:DNA