title: [The effect of methotrexate on phenobarbital induction of monooxygenases and UDP-glucuronyltransferases in rat liver].
abstract: Methotrexate (N10-methyl-4-amino-4-deoxyfolic acid), administered to rats subcutaneously at a dose of 0.25 mg/kg within 14 days, was found to decrease content of microsomal protein in liver tissue and content of cytochromes P-450 and b5 as well as the drug decreased activity of amidopyrine- and ethylmorphine-N-demethylases, aniline-p-hydroxylase, UDP-glucuronosyltransferase and urinary excretion of glucuronides by 21%, 60%, 39%, 33%, 53%, 21%, 41% and 28%, respectively. Phenobarbital, administered subcutaneously at a dose of 60 mg/kg within 14 days, elevated the parameters by 28%, 187%, 63%, 163%, 162%, 100%, 95% and 60%, respectively. The effect of phenobarbital in combination with methotrexate was decreased by 77%, 49%, 55%, 52%, 43%, 42% and 22%, respectively.
authors.forename: P I
authors.initials: PI
authors.lastname: Lukienko
authors.forename: L F
authors.initials: LF
authors.lastname: Legon'kova
authors.forename: M I
authors.initials: MI
authors.lastname: Bushma
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Shoka
authors.forename: I V
authors.initials: IV
authors.lastname: Zverinskiĭ
authors.forename: L B
authors.initials: LB
authors.lastname: Zavodnik
mesh_terms: D000818:Animals; D004790:Enzyme Induction; D005494:Folic Acid Deficiency / Q000201:enzymology; D005965:Glucuronates / Q000652:urine; D014453:Glucuronosyltransferase / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D010634:Phenobarbital / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats
chemical_list: D005965:Glucuronates; D006899:Mixed Function Oxygenases; D014453:Glucuronosyltransferase; D008727:Methotrexate; D010634:Phenobarbital
vernacular_title: Vliianie metotreksata na fenobarbitalovuiu induktsiiu monoksigenaz i UDF-gliukuronoziltransferazy pecheni krys.