Molecular cloning of rat liver glucocorticoid-receptor translocation promoter.
We previously found a new endogenous factor in rat liver cytosol, named ATP-stimulated glucocorticoid-receptor translocation promoter (ASTP), that increased the binding of activated glucocorticoid-receptor to nuclei in the presence of ATP. We have cloned and sequenced a cDNA (2989 bp) encoding the ASTP protein. An open reading frame of 524 amino acids encodes a protein with a molecular weight of 57 kDa. Amino-acid sequences of six peptides obtained by Edman degradation of various cleavage products of the purified ASTP were identified in the cDNA-derived sequence. Northern analysis of mRNA from rat liver revealed a major band of about 3.8 kb. Database searches show no homology to other known proteins from mammalian origin, however, the amino-acid sequence in the middle portion of ASTP protein shows 48% homology to glycerol kinase from Escherichia coli. The region of homology to other species may help to define sites of protein-protein interaction, as well as sites of possible interaction of ASTP with ATP.
Okamoto
K
K
Department of Oncology, Osaka University Medical School, Japan.
Hirano
H
H
Isohashi
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D005991:Glycerol Kinase; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D004247:DNA; C485945:Gk protein, rat; D005991:Glycerol Kinase