Protein kinase C in galactosemic and tolrestat-treated lens epithelial cells.
Using isozyme-specific anti-peptide antisera against peptides from the alpha-, beta-, gamma-, delta-, epsilon-, and zeta-isoforms of brain protein kinase C (PKC), we have identified proteins in bovine lens epithelial cells, in culture, that were reactive with these antisera. Western blots of lens epithelial cell homogenates showed that PKC-alpha antisera reacted with a major protein, and PKC-gamma antisera reacted with a minor protein. When the lens epithelial cells were cultured in media supplemented with 40 mM galactose, to model the conditions of sugar cataracts, a decrease in PKC-gamma, but not in PKC-alpha was observed. These were normalized if the cells were cultured in 40 mM galactose media supplemented with an inhibitor of aldose reductase, Tolrestat (10 microM). These results suggest that changes in PKC isoforms occur in the galactosemic diabetic state.
Gonzalez
K
K
Department of Biology, Kansas State University, Manhattan 66506.
Udovichenko
I
I
Cunnick
J
J
Takemoto
D J
DJ
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002386:Cataract / Q000201:enzymology; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D005690:Galactose / Q000494:pharmacology*; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism*; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology*; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000494:pharmacology*; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism*
D003470:Culture Media; D007527:Isoenzymes; D009281:Naphthalenes; D010455:Peptides; C040550:tolrestat; D000449:Aldehyde Reductase; D011493:Protein Kinase C; D005690:Galactose