Determination of aspoxicillin in broncho-alveolar lavage fluid by high-performance liquid chromatography with photolysis and electrochemical detection.
A determination method for the penicillin antibiotic aspoxicillin in broncho-alveolar lavage fluid has been developed, involving high-performance liquid chromatography and post-column photolysis. The method enabled the determination of aspoxicillin at concentrations below 100 ng/ml, with a linear calibration curve at concentrations of 1-1000 ng/ml for 100 microliters of lavage fluid. The detection limit was 1 ng/ml at a signal-to-noise ratio of 3:1.
Yamazaki
T
T
Marugo Laboratory Service Center, Osaka, Japan.
Ishikawa
T
T
Nakai
H
H
Miyai
M
M
Tsubota
T
T
Asano
K
K
D000658:Amoxicillin / Q000031:analogs & derivatives* / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet
C037485:aspoxicillin; D000658:Amoxicillin