High throughput DNA sequencing using an automated electrophoresis analysis system and a novel sequence assembly program.
The combination of high-resolution scanning, image-databasing technology and sequence assembly software makes it possible to assemble contiguous overlapping sequences of DNA in a fraction of the time required by manual methods. This paper describes and evaluates an assembly program that rapidly generates contigs from scanned images and provides a unique ability to verify disagreements between overlapping strands or fragments of complementary DNA sequences on-screen, further increasing sequencing throughput.
Smith
S
S
Millipore Corporation, Burlington, MA 01803.
Welch
W
W
Jakimcius
A
A
Dahlberg
T
T
Preston
E
E
Van Dyke
D
D
D001483:Base Sequence; D004586:Electrophoresis; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software