[The detection of the location of glucose-6-phosphate dehydrogenase in the fibroblasts of voles and mice and in rat myoblasts by using monoclonal antibodies against glucose-6-phosphate dehydrogenase].
Monoclonal antibodies (MAs) were produced against glucose-6-phosphate dehydrogenase (G6PD) of two vole species--Microtus arvalis and M. subarvalis. The binding level of the MAs to G6PD in both species were almost the same, which suggested that these MAs may be specific for the antigenic determinants common to G6PD of these species. The MAs produced against the vole G6PD were used for its intracellular localization. The patterns obtained after staining cells with the use of MAs against G6PD were the same as those obtained after staining with the use of antibodies against F-actin. There was a good conformity between the results of light and electron microscopic immunoenzyme analyses with regard to the binding of MAs produced to the actin microfilaments. It is concluded that G6PD is closely associated with actin microfilaments of the cell cytoskeleton.
Zakiian
S M
SM
Nesterova
T B
TB
Cheriaukene
O V
OV
Matveeva
N M
NM
Bochkarev
M N
MN
Baĭborodin
S I
SI
Kerkis
A Iu
AIu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D003411:Arvicolinae; D002460:Cell Line; D002478:Cells, Cultured / Q000201:enzymology / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005347:Fibroblasts / Q000201:enzymology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D016253:Microscopy, Immunoelectron; D009132:Muscles / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats
D000911:Antibodies, Monoclonal; D005954:Glucosephosphate Dehydrogenase
Vyiavlenie lokalizatsii gliukozo-6-fosfatdegidrogenazy v fibroblastakh polevok i myshi i v mioblastakh krysy s pomoshch'iu monoklonal'nykh antitel protiv gliukozo-6-fosfatdegidrogenazy.