Effect of denervation on overdevelopment of chloroquine-induced autophagic vacuoles in skeletal muscles.
We studied how denervation affects the overdevelopment of autophagic vacuoles in muscles of chloroquine-treated rats. The number of autophagic vacuoles increased significantly in the chloroquine-treated soleus muscles after denervation as compared to similarly treated contralateral, innervated muscles. No vacuoles were present in the denervated and innervated muscles of saline-treated rats. After denervation, the autophagic vacuoles in chloroquine-treated muscle contained numerous glycogen particles and various heterogeneous materials. A biochemical study showed no significant difference in the activities of lysosomal proteases and hydrolases in the chloroquine- and saline-treated muscles after denervation, although these activities were markedly increased in comparison to the same activities in the contralateral, innervated muscles. Chloroquine treatment by itself did not, but denervation with or without chloroquine treatment did enhance the biochemical activities of lysosomal enzymes in the animals. We speculate that denervation induces the marked accumulation of autophagic vacuoles in chloroquine-induced myopathy.
Kumamoto
T
T
First Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Ueyama
H
H
Watanabe
S
S
Murakami
T
T
Araki
S
S
D000818:Animals; D001343:Autophagy; D002738:Chloroquine / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism; D006651:Histocytochemistry; D006867:Hydrolases / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009121:Muscle Denervation; D009132:Muscles / Q000187:drug effects* / Q000294:innervation / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D014617:Vacuoles / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure
D002738:Chloroquine; D006867:Hydrolases; D010450:Endopeptidases