title: The cooperation of adipocytes and stromal cells in the secretion of prostaglandins by rat adipose tissue is not influenced by diet.
abstract: This study examined the influence of dietary essential fatty acids on the cooperativity of isolated adipocytes and stromal-vascular cells in the biosynthesis of prostaglandins. Sprague-Dawley rats were fed either a diet rich in essential fatty acids (20% corn oil) or a diet poor in essential fatty acids (20% tallow) for 4 wk. Preparations containing primarily adipose cells (adipocytes) or stromal-vascular cells (nonfat cells) were obtained from epididymal fat pads by collagenase digestion and repeatedly washed. Prostaglandin production was evaluated in basal and epinephrine-stimulated media after incubation with either adipocytes or adipocytes+nonfat cells. Prostaglandin E2 and prostacyclin production by adipocytes+nonfat cells was dose-dependent with epinephrine stimulation in cells from rats fed both diets. Both prostaglandin E2 and glycerol release in response to epinephrine (10-100 mumol/L) stimulation from adipocytes or from adipocytes+nonfat cells were significantly higher for cells from corn oil-fed rats than for cells from tallow-fed rats. Regardless of dietary treatment, epinephrine-stimulated prostaglandin E2 and prostacyclin release from adipocytes+nonfat cells was much greater than from adipocytes. These results suggest that a diet high in essential fatty acids precipitates a higher prostaglandin E2 secretion and that nonfat cells potentiate the secretion of prostaglandin E2 and prostacyclin by adipocytes regardless of diet.
authors.affiliation: Department of Foods and Nutrition, University of Georgia, Athens 30602.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: D B
authors.initials: DB
authors.lastname: Hausman
authors.forename: G J
authors.initials: GJ
authors.lastname: Hausman
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis*; D004837:Epinephrine / Q000494:pharmacology; D011464:Epoprostenol / Q000096:biosynthesis*; D005228:Fatty Acids, Essential / Q000008:administration & dosage / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005228:Fatty Acids, Essential; D011464:Epoprostenol; D015232:Dinoprostone; D004837:Epinephrine