Unique expression pattern of protein kinase C-theta: high mRNA levels in normal mouse testes and in T-lymphocytic cells and neoplasms.
A 2.2-kb cDNA that contains the entire coding region of mouse protein kinase C-theta (PKC-theta) was cloned from skeletal muscle mRNA using reverse transcription and the polymerase chain reaction (PCR). This clone was used as a probe to study the expression of this PKC isoform in normal and transformed hemopoietic cells and other normal tissues. By far the highest steady-state level of PKC-theta mRNA was found as a 2.8-kb transcript on a Northern blot of poly(A)+ RNA from testes. High levels were also found in skeletal muscle, spleen, T lymphomas and purified normal T lymphocytes, but these tissues and cells expressed two transcripts, 3.3 kb and 3.8 kb. Lower levels of similar size transcripts were found in normal brain, B lymphocytes and B-lymphocytic tumors and cell lines.
Mischak
H
H
Molecular Genetics Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Goodnight
J
J
Henderson
D W
DW
Osada
S
S
Ohno
S
S
Mushinski
J F
JF
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D015342:DNA Probes; D015870:Gene Expression; D008223:Lymphoma / Q000201:enzymology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000201:enzymology*; D013737:Testis / Q000201:enzymology*
D015342:DNA Probes; D012333:RNA, Messenger; D011493:Protein Kinase C