title: The role of the beta-subunit of initiation factor eIF-2 in initiation complex formation.
abstract: The functional properties of preparations of protein synthesis initiation factor eIF-2 which lack the beta-subunit (as confirmed immunologically) were compared with those of the heterotrimeric factor. The former can bind guanine nucleotides but not initiator tRNA, and also exhibits a substantially reduced rate of initiation factor eIF-2B-mediated GDP/GTP-exchange.
authors.affiliation: Department of Biochemistry, School of Medical Sciences, University of Bristol, UK.
authors.forename: A
authors.initials: A
authors.lastname: Flynn
authors.forename: S
authors.initials: S
authors.lastname: Oldfield
authors.forename: C G
authors.initials: CG
authors.lastname: Proud
mesh_terms: D000818:Animals; D015852:Eukaryotic Initiation Factor-2 / Q000737:chemistry / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors; D006153:Guanosine Diphosphate / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012358:RNA, Transfer, Met / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
chemical_list: D015852:Eukaryotic Initiation Factor-2; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D012358:RNA, Transfer, Met; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate