Oxidative damage to DNA: levels of thymine glycol and thymidine glycol in neoplastic human urines.
We have measured the levels of thymine glycol (TG) and thymidine glycol (dTG) in human urine, using an HPLC method. The results show that all 30 specimens examined (including 10 non-neoplastic and 20 neoplastic human urines) contained significant amounts of TG and dTG, average levels (mean +/- SEM) were 0.376 +/- 0.026 and 0.138 +/- 0.009 nmol/kg body weight/day respectively. The average levels of TG and dTG were 0.435 +/- 0.038 and 0.164 +/- 0.017 nmol/kg body weight/day in 10 healthy human urine specimens and 0.347 +/- 0.035 and 0.125 +/- 0.010 nmol/kg body weight/day in 20 neoplastic human urine specimens respectively. No significant differences were found between female and male as well as between the non-neoplastic and neoplastic human urine specimens. There were also wide interindividual variations, which were not age-dependent.
Cao
E H
EH
Institute of Biophysics, Academia Sinica, Beijing, China.
Wang
J J
JJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000652:urine*; D010084:Oxidation-Reduction; D013936:Thymidine / Q000031:analogs & derivatives* / Q000652:urine; D013941:Thymine / Q000031:analogs & derivatives* / Q000652:urine
C029389:thymine glycol; C029390:thymidine glycol; D013941:Thymine; D013936:Thymidine