A new automated method for isolation of genomic DNA from eukaryotic cells.
In this study we determine whether the BIOMEK-1000 Laboratory Workstation (Beckman Instruments, Fullerton, CA) can be used to isolate genomic DNA from eukaryotic cells. The results obtained demonstrate that DNA isolated by BIOMEK-1000 is suitable for amplification of genomic sequences by PCR. Our data prove that automated chromatographic DNA isolation employing the BIOMEK-1000 could be used in molecular diagnosis of human pathologies.
Mischiati
C
C
Istituto di Chimica Biologica, Università di Ferrara.
Feriotto
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G
Gambari
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R
D001331:Automation; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D015343:DNA Probes, HLA; D004273:DNA, Neoplasm / Q000302:isolation & purification; D005057:Eukaryotic Cells / Q000737:chemistry*; D006801:Humans; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D015343:DNA Probes, HLA; D004273:DNA, Neoplasm; D004247:DNA