title: Extreme temporary thinning after keratoplasty of organ-cultured corneas.
abstract: Many investigators have measured corneal thickness after keratoplasty using organ culture and storage mediums. Values for corneal thickness were found to stabilize 6-12 months postoperatively, reaching normal values. We followed 59 corneal transplant recipients whose donor tissue was obtained from organ culture (average preservation time 13 days) for > 1 year retrospectively. The overall graft survival was 95.3%. The graft was thick in the early postoperative period. It subsequently became thinner, reaching the lowest level 5-9 months postoperatively (mean 0.48 mm, SD 0.04). After this period the corneas gradually became thicker and reached the normal corneal thickness. The extreme thinning of transplanted corneas in the first postoperative year has not been described before. We found a negative correlation between conservation time and thickness 5-9 months postoperatively. We postulate that organ culture may have a high impact on keratocyte function.
authors.affiliation: Department of Ophthalmology, University of Amsterdam, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Geerards
authors.forename: J H
authors.initials: JH
authors.lastname: Kok
mesh_terms: D003315:Cornea / Q000166:cytology / Q000473:pathology*; D003470:Culture Media; D005260:Female; D005500:Follow-Up Studies; D006085:Graft Survival; D006801:Humans; D006984:Hypertrophy / Q000209:etiology; D015948:Keratoplasty, Penetrating / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques / Q000009:adverse effects; D012189:Retrospective Studies
chemical_list: D003470:Culture Media