title: Evaluation of intraocular penetration of topically administered tissue plasminogen activator in dogs.
abstract: Topically administered tissue plasminogen activator (tPA) was evaluated for its penetration into aqueous humor of clinically normal dogs. Two concentrations of tPA (5 mg/ml and 10 mg/ml) were evaluated in a single-dose study, and a concentration of 5 mg of tPA/ml was used for a multiple-dose study. The contralateral eye served as a nontreated control. Enzyme substrate analysis of aqueous humor was used to determine tPA activity. The activity of tPA in aqueous humor was significantly (P < or = 0.05) greater in treated eyes of all dogs, compared with that in control eyes. Significant differences in activity of tPA were not detected at different doses in treated eyes.
authors.affiliation: Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana 61801.
authors.forename: P A
authors.initials: PA
authors.lastname: Gerding
authors.forename: T E
authors.initials: TE
authors.lastname: Eurell
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D001681:Biological Assay; D004285:Dogs / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005260:Female; D008297:Male; D009883:Ophthalmic Solutions; D010959:Tissue Plasminogen Activator / Q000493:pharmacokinetics*
chemical_list: D009883:Ophthalmic Solutions; D010959:Tissue Plasminogen Activator