The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and EcoHI.
The restriction endonucleases Eco1831I and EcoHI cleave before the first 5'-cytosine in the recognition sequence 5'-decreases CCSGG--3'/3'--GGSCC increases-5' (where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA+ hosts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine.
Kravetz
A N
AN
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region.
Zakharova
M V
MV
Beletskaya
I V
IV
Sineva
E V
EV
Denjmuchametov
M M
MM
Petrov
S I
SI
Glatman
L I
LI
Solonin
A S
AS
D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D005798:Genes, Bacterial; D013379:Substrate Specificity
D015252:Deoxyribonucleases, Type II Site-Specific