The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system.
The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich region detected at the C terminus of the FTF of S. salivarius preceded a hydrophobic terminal domain. This proline-rich region was shown to possess strong homology to the product of the prgC gene from pCF10 in Enterococcus faecalis, which encodes a pheromone-responsive protein of unknown function, as well as homology to the human proline-rich salivary protein PRP-4. A series of 3'-OH deletions of the S. salivarius ftf gene expressed in Streptococcus gordonii Challis LGR2 showed that the C terminus was required for cell surface attachment in this heterologous organism, as only the complete gene product was cell bound. This cell-bound activity was released in the presence of sucrose, suggesting that the mode of attachment and release of the S. salivarius FTF in S. gordonii was similar to that in its native host.
Rathsam
C
C
Institute of Dental Research, Australia.
Giffard
P M
PM
Jacques
N A
NA
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D013293:Enterococcus faecalis / Q000235:genetics; D005798:Genes, Bacterial; D006602:Hexosyltransferases / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D055232:Proline-Rich Protein Domains; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012270:Ribosomes / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins
D006602:Hexosyltransferases; D010455:Peptides; D012471:Salivary Proteins and Peptides; C021513:levansucrase; C021451:inulosucrase; D001426:Bacterial Proteins