title: GH3 cells, an anterior pituitary cell line, express CCK-B receptors.
abstract: We found that GH3 cells, a rat anterior pituitary tumor cell line, expressed a single class of high-affinity binding sites for radiolabeled cholecystokinin octapeptide (CCK-8) with a Kd of 48 pM. The binding sites had high affinity for CCK-8, CCK-4, gastrin I, and L-365,260 (CCK-B antagonist), and had low affinity for devazepide (CCK-A antagonist), indicating that the binding sites are CCK-B receptors. GTP and its stable analogues inhibited radiolabeled CCK-8 binding to GH3 cell membranes, suggesting a coupling of CCK-B receptors to a G-protein.
authors.affiliation: Department of Pharmaceutical Screening, Nippon Roche Research Center, Kamakura, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kuwahara
authors.forename: M
authors.initials: M
authors.lastname: Takamiya
authors.forename: H
authors.initials: H
authors.lastname: Nagase
authors.forename: T
authors.initials: T
authors.lastname: Kudoh
authors.forename: A
authors.initials: A
authors.lastname: Nakano
authors.forename: H
authors.initials: H
authors.lastname: Yoshizaki
authors.forename: M
authors.initials: M
authors.lastname: Arisawa
mesh_terms: D000818:Animals; D010903:Pituitary Gland, Anterior / Q000737:chemistry* / Q000166:cytology; D011869:Radioligand Assay; D051381:Rats; D011949:Receptors, Cholecystokinin / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D011949:Receptors, Cholecystokinin