title: Determination of a new antifilarial drug, UMF-058, and mebendazole in whole blood by high-performance liquid chromatography.
abstract: A rapid and selective high-performance liquid chromatographic assay for simultaneous quantitative determination of a new antifilarial drug (UMF-058, I) and mebendazole (MBZ) is described. After a simple extraction from whole blood, both compounds were analysed using a C18 Nova Pak reversed-phase column and a mobile phase of methanol-0.05 M ammonium dihydrogenphosphate (50:50, v/v) adjusted to pH 4.0, with ultraviolet detection at 291 nm. The average recoveries of I and MBZ over a concentration range of 25-250 ng/ml were 92.0 +/- 7.7 and 84.4 +/- 4.4%, respectively. The minimum detectable concentrations in whole blood for I and MBZ were 7 and 6 ng/ml, respectively. This method was found to be suitable for pharmacokinetic studies.
authors.affiliation: Centre for Drug Research, Universiti Sains Malaysia Malaysia, Penang.
authors.forename: S
authors.initials: S
authors.lastname: Ramanathan
authors.forename: N K
authors.initials: NK
authors.lastname: Nair
authors.forename: S M
authors.initials: SM
authors.lastname: Mansor
authors.forename: V
authors.initials: V
authors.lastname: Navaratnam
mesh_terms: D000818:Animals; D017178:Brugia malayi; D002520:Cercopithecidae; D002851:Chromatography, High Pressure Liquid; D005368:Filariasis / Q000097:blood / Q000188:drug therapy; D005369:Filaricides / Q000097:blood* / Q000493:pharmacokinetics; D006207:Half-Life; D008463:Mebendazole / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005369:Filaricides; C082258:methyl-(5-methoxybenzyl-1H-benzimidazol-2-yl)carbamate; D008463:Mebendazole