title: [Seroprevalence of hepatitis C antibodies among blood donors. Study of second generation ELISA and RIBA tests and surrogate markers].
abstract: Between March and November 1991, prevalence of hepatitis C antibodies was evaluated in 60,960 blood donors from the North-East of France. Using a second generation ELISA, 424 donors (0.69%) were reactive, with no significant difference between males (0.69%) and females (0.70%). Among these 424 donors, respectively 137 (32.3%), 86 (20.3%) and 201 (47.4%) were reactive, indeterminate or nonreactive by a second generation RIBA (RIBA-2) (Recombinant Immunoblot Assay). Donors with a high ELISA ratio (> or = 4) were significantly more likely to have a reactive RIBA-2. Of the 1906 donors with anti-HBc positivity (3.12%), 44 had a reactive ELISA; of these, respectively 27, 12 and 5 had a reactive, indeterminate and nonreactive RIBA. Of the 1201 donors (1.97%) with increased serum ALAT (alanine-amino-transferase) levels (> or = 2N), 42 had a positive ELISA; of these, respectively 35, 2 and 5 had a reactive, indeterminate and nonreactive RIBA. Of the 54 donors with both indirect markers, nine had a reactive ELISA; the same nine donors had a reactive RIBA. These data show that donors with both surrogate markers and a reactive ELISA are very likely to have a positive RIBA. Seventy-seven (18.16%) of the 424 donors with a reactive ELISA had at least one surrogate marker; 67 of these donors (30.04%) were among the 223 donors with a reactive ELISA and a reactive or indeterminate RIBA.
authors.affiliation: Centre Régional de Transfusion Sanguine de Nancy, Vandceuvre-les-Nancy, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Aymard
authors.forename: C
authors.initials: C
authors.lastname: Botte
authors.forename: P
authors.initials: P
authors.lastname: Contal
authors.forename: C
authors.initials: C
authors.lastname: Janot
authors.forename: F
authors.initials: F
authors.lastname: Streiff
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005602:France / Q000453:epidemiology; D006508:Hepatitis Antibodies / Q000032:analysis*; D006510:Hepatitis B Antibodies / Q000032:analysis; D006526:Hepatitis C / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D015995:Prevalence
chemical_list: D015415:Biomarkers; D006508:Hepatitis Antibodies; D006510:Hepatitis B Antibodies
vernacular_title: Séroprévalence des anticorps contre le virus de l'hépatite c chez les donneurs de sang. Etude des tests ELISA et RIBA de 2e génération et des marqueurs indirects.