Marked reduction of acyl-CoA synthetase activity and mRNA in intra-abdominal visceral fat by physical exercise.
Several reports have suggested that the reduction of intra-abdominal visceral fat after physical exercise is more prominent than that of subcutaneous fat. We compared some parameters in mesenteric and subcutaneous fats between sedentary and exercised rats (treadmill running; 10-20 m/min, 60 min/day, 7 days). Tissue weight and cell volume were decreased in mesenteric fat by the exercise. The exercise reduced activity and mRNA levels of acyl-CoA synthetase (ACS; 67 and 26% of those of the sedentary group, respectively), mRNA levels of lipoprotein lipase (LPL; 49% of those of the sedentary group), and GLUT-4 (38% of those of the sedentary group) in the mesenteric fat. In contrast, all of these parameters did not change significantly in the subcutaneous fat. Gastrocnemius muscle was heavier in exercised rats. ACS activity was elevated in the gastrocnemius muscle of the exercised rats (137% of those of sedentary group), although mRNA levels of ACS, LPL, and GLUT-4 did not change in the muscle by the exercise. These observations suggest that mesenteric fat may contribute to switching of distribution of plasma energy flux, including lipid and glucose, from fat tissue to muscle in physical exercise.
Shimomura
I
I
Second Department, Osaka University Medical School, Japan.
Tokunaga
K
K
Kotani
K
K
Keno
Y
Y
Yanase-Fujiwara
M
M
Kanosue
K
K
Jiao
S
S
Funahashi
T
T
Kobatake
T
T
Yamamoto
T
T
D000005:Abdomen; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism*; D051275:Glucose Transporter Type 4; D008071:Lipoprotein Lipase / Q000235:genetics; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D009124:Muscle Proteins; D005082:Physical Exertion; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014781:Viscera; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C495694:Slc2a4 protein, rat; D008071:Lipoprotein Lipase; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase