Transcripts for cysteine-rich secretory protein-1 (CRISP-1; DE/AEG) and the novel related CRISP-3 are expressed under androgen control in the mouse salivary gland.
Expression of the gene for cysteine-rich secretory protein-1 (CRISP-1), the counterpart of the rat sperm-coating protein DE/AEG, was evidenced in male mouse salivary gland by both isolation of the corresponding cDNA clone and RNA blot analysis. In addition, the cDNA coding for the new related CRISP-3, which shares 77% amino acid identity with CRISP-1, was isolated from a salivary gland cDNA library. The activity of CRISP-1 and -3 genes in mouse salivary gland is strongly androgen dependent, as seen in castrated males; also, no CRISP-1 and only weak CRISP-3 expression was detected in female salivary gland. A variety of tissues were tested for the presence of CRISP-3 transcripts, but specific signals were only found in salivary gland. The results of genomic DNA blot analysis are compatible with the existence of single discrete genes for CRISP-1 and CRISP-3.
Haendler
B
B
Research Laboratories of Schering AG, Berlin, Germany.
Krätzschmar
J
J
Theuring
F
F
Schleuning
W D
WD
D000595:Amino Acid Sequence; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012469:Salivary Glands / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000235:genetics*; D029589:Seminal Plasma Proteins; D017385:Sequence Homology; D014018:Tissue Distribution; D014158:Transcription, Genetic
D000728:Androgens; C489926:CRISP3 protein, human; C486321:Crisp1 protein, mouse; C489927:Crisp3 protein, mouse; C486320:Crisp3 protein, rat; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins; D004247:DNA