Editing of rps3/rpl16 transcripts creates a premature truncation of the rpl16 open reading frame.
Overlapping open reading frames corresponding to maize mitochondrial genes rps3 and rpl16 have been found in Petunia mitochondrial DNA. The DNA region associated with these two genes is part of the Petunia mitochondrial recombination repeat and is iterated three times. Analysis of transcripts from these genes shows that there is RNA editing of the coding regions and that one of the editing sites detected in the open reading frame overlap creates a premature stop codon in the rpl16 sequence. No transcripts were detected that were unedited at this site. Thus, in Petunia editing of rpl16 appears to render this gene nonfunctional.
Sutton
C A
CA
Section of Genetics and Development, Cornell University, Ithaca, NY 14853.
Conklin
P L
PL
Pruitt
K D
KD
Calfee
A J
AJ
Cobb
A G
AG
Hanson
M R
MR
D000595:Amino Acid Sequence; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants; D014176:Protein Biosynthesis; D017393:RNA Editing; D013728:Terminator Regions, Genetic
D004272:DNA, Mitochondrial; D010940:Plant Proteins; C082042:YRPL16 protein, Petunia hybrida; C082041:YRPS3 protein, Petunia hybrida