Use of a thick-film capillary column for the analysis of organic acids in body fluids.
An improved method for the identification and quantification of organic acids in body fluids employing capillary gas chromatography-mass spectrometry has been developed. A thick-film capillary column, that combines the properties of a capillary column with those of a megabore column, has been successfully introduced into an existing method. Analysis over a concentration range from 1 mumol/l to 500 mumol/l body fluid is possible. This permits the assay of samples that are usually obtained in small volumes, e.g. cerebrospinal fluid.
Meier-Augenstein
W
W
Abteilung Neuropädiatrie, Universitäts-Kinderklinik, Heidelberg, Germany.
Hoffmann
G F
GF
Holmes
B
B
Jones
J L
JL
Nyhan
W L
WL
Sweetman
L
L
D000143:Acids / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D005998:Glycine / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents
D000143:Acids; D007202:Indicators and Reagents; D005998:Glycine