An improved method for typing of plasma alpha 1-protease inhibitor (PI1) variants in sheep.
Sheep plasma alpha 1-protease inhibitor (PI1) variants were typed by analysing neuraminidase treated samples by isoelectric focusing in ultrathin polyacrylamide gels followed by staining for trypsin inhibition. The 10 different PI1 phenotypes observed were shown to be controlled by four codominant alleles. The probability of sire exclusion provided by PI1 polymorphism was about 0.45 in both of the studied milk sheep breeds (Latxa and Karrantzar) of the Basque Country.
Estomba
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A
Department of Animal Biology and Genetics, Faculty of Sciences, University of the Basque Country, Bilbao, Spain.
Basaras
M
M
Aguirre
A
A
Vicario
A
A
Jugo
B
B
Mazon
L I
LI
D000483:Alleles; D000818:Animals; D001947:Breeding; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005787:Gene Frequency; D007525:Isoelectric Focusing / Q000662:veterinary; D009439:Neuraminidase; D011110:Polymorphism, Genetic; D012756:Sheep / Q000235:genetics*; D000515:alpha 1-Antitrypsin / Q000235:genetics*
D000515:alpha 1-Antitrypsin; D009439:Neuraminidase