Gene manipulation by protoplast fusion and penicillin production by Penicillium chrysogenum.
Hybrids have been obtained by protoplast fusion of nitrate-nonutilizing cnx- and acetate-nonutilizing fac- mutants of Penicillium chrysogenum strains 4/95 and 26/818, respectively. Induced haplodization of the hybrids allowed the recovery of stable segregants, which were screened for penicillin production. The penicillin-producing segregants showed a wide range of titers that reached for a certain mutant to 290-390% increase above the parent strains.
Tahoun
M K
MK
Department of Agricultural Industries, Faculty of Agriculture, University of Alexandria, Egypt.
D002704:Chlorates / Q000494:pharmacology; D005463:Fluoroacetates / Q000494:pharmacology; D009154:Mutation; D010406:Penicillins / Q000096:biosynthesis*; D010408:Penicillium chrysogenum / Q000235:genetics / Q000378:metabolism*; D011523:Protoplasts
D002704:Chlorates; D005463:Fluoroacetates; D010406:Penicillins; C014815:fluoroacetic acid