title: Clones of T cells discriminate between native and deglycosylated forms of MHC class II antigen in allostimulation.
abstract: The aim of this study was to clarify the role of the oligosaccharide side chains of MHC Class II antigens in allostimulation. The approach was to cleave the oligosaccharides from protein by subjecting plasma membranes (PM) of the Daudi cell line to chemical deglycosylation yielding deglycosylated (dgl) proteins and a supernatant fraction containing plasma membrane oligosaccharides (dgl sup). MHC Class II antigens affinity purified from the native and the dgl PM were inserted into the plasma membrane of peripheral blood leukocytes (PBL) used as stimulators in a mixed leukocyte reaction (MLR). Cells used as stimulators and as responders were from the same donor. Both native and to a lesser extent the dgl antigen could elicit a proliferative as well as a cytolytic (CML) response. A comparable reduction in the CML reaction was also obtained when native antigen was used to elicit effector cells, but the target was stripped of N-linked oligosaccharides by pretreatment with tunicamycin (TM). Five clones of responding cells raised against the native antigen were studied. Two gave proliferative reactions of equal magnitude to native and to dgl antigen alike, while three responded only to the native form. These three clones did not lyse TM-treated target cells. Inhibition experiments of CML were performed with either the dgl sup containing Daudi PM oligosaccharides or with an anti MHC-Class II MoAb. CML reactivity of the three clones which responded to native antigen was blocked by the dgl sup but not by the anti-MHC antibody. Conversely, the reaction of the two clones reactive to both forms of antigen was only inhibited by the anti-MHC antibody using intact or TM-treated targets. Accordingly, in terms of the latter set of clones oligosaccharide side chains of MHC may not be required for allostimulation. Data obtained with the set of three clones suggest that oligosaccharides could act as target of cytotoxic T cells.
authors.affiliation: Department of Surgery, McGill University, Montreal, Quebec, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Culley
authors.forename: D
authors.initials: D
authors.lastname: Waldron-Edward
authors.forename: P
authors.initials: P
authors.lastname: Manjunath
authors.forename: O A
authors.initials: OA
authors.lastname: Mamer
authors.forename: K
authors.initials: K
authors.lastname: Abikar
authors.forename: H
authors.initials: H
authors.lastname: Rode
authors.forename: J
authors.initials: J
authors.lastname: Gordon
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006031:Glycosylation; D006681:HLA-D Antigens / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D009844:Oligosaccharides / Q000276:immunology*; D014415:Tunicamycin
chemical_list: D006681:HLA-D Antigens; D009844:Oligosaccharides; D014415:Tunicamycin