title: Extent of peptidoglycan O acetylation in the tribe Proteeae.
abstract: The degree of peptidoglycan O acetylation in 18 strains of the different genera of the tribe Proteeae (Proteus, Providencia, and Morganella) has been determined by high-performance liquid chromatography-based organic acid analysis of mild-base-released acetic acid and quantitation of peptidoglycan concentrations by simultaneous amino sugar-amino acid analysis using high-performance anion-exchange chromatography with pulsed amperometric detection. The N,O-diacetylmuramyl content of all isolated and purified peptidoglycans was greater than 29% and ranged up to 57% relative to total muramic acid concentration. Each of the O-acetylated peptidoglycans was found to be resistant to solubilization by hen egg white lysozyme.
authors.affiliation: Department of Microbiology, University of Guelph, Ontario, Canada.
authors.forename: A J
authors.initials: AJ
authors.lastname: Clarke
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D004755:Enterobacteriaceae / Q000737:chemistry / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry* / Q000302:isolation & purification; D011511:Proteus / Q000737:chemistry / Q000378:metabolism*; D011532:Providencia / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D010457:Peptidoglycan; D009113:Muramidase