Rapid detection of infectious mononucleosis-associated heterophile antibodies by a novel immunochromatographic assay and a latex agglutination test.
A novel immunochromatographic assay, the CARDS O.S. MONO test (Pacific Biotech, San Diego, Calif.), and a latex agglutination test, the Infectious Mononucleosis Kit (Unipath Ltd., Hampshire, United Kingdom) were compared with the Paul-Bunnell-Davidsohn test. Of the 957 serum specimens studied, 78 were positive and 879 were negative by the Paul-Bunnell-Davidsohn test. After discrepancies were resolved by determining Epstein-Barr virus serology, the sensitivities of the CARDS O.S. MONO test and the Infectious Mononucleosis Kit were 91.0 and 96.2%, respectively, and both tests had a specificity and a positive predictive value of 100% and a negative predictive value and overall agreement of greater than 99%. The results show that both tests can accurately detect infectious mononucleosis-associated heterophile antibodies.
Farhat
S E
SE
Department of Microbiology, Daniel Medical Laboratories, Downsview, Ontario, Canada.
Finn
S
S
Chua
R
R
Smith
B
B
Simor
A E
AE
George
P
P
Diena
B B
BB
Diena
D
D
Skulnick
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000910:Antibodies, Heterophile / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002845:Chromatography; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000706:statistics & numerical data; D007244:Infectious Mononucleosis / Q000175:diagnosis* / Q000276:immunology; D007841:Latex Fixation Tests / Q000706:statistics & numerical data; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000910:Antibodies, Heterophile