title: Use of a thick-film capillary column for the analysis of organic acids in body fluids.
abstract: An improved method for the identification and quantification of organic acids in body fluids employing capillary gas chromatography-mass spectrometry has been developed. A thick-film capillary column, that combines the properties of a capillary column with those of a megabore column, has been successfully introduced into an existing method. Analysis over a concentration range from 1 mumol/l to 500 mumol/l body fluid is possible. This permits the assay of samples that are usually obtained in small volumes, e.g. cerebrospinal fluid.
authors.affiliation: Abteilung Neuropädiatrie, Universitäts-Kinderklinik, Heidelberg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Meier-Augenstein
authors.forename: G F
authors.initials: GF
authors.lastname: Hoffmann
authors.forename: B
authors.initials: B
authors.lastname: Holmes
authors.forename: J L
authors.initials: JL
authors.lastname: Jones
authors.forename: W L
authors.initials: WL
authors.lastname: Nyhan
authors.forename: L
authors.initials: L
authors.lastname: Sweetman
mesh_terms: D000143:Acids / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D005998:Glycine / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents
chemical_list: D000143:Acids; D007202:Indicators and Reagents; D005998:Glycine