[The modification of Cu, Zn-superoxide dismutase by monomethoxypolyethylene glycol improves the indices of the experimental therapy of the ischemic myocardium in rats].
Mice were used to make a comparative study of the biological distribution of intravenous preparations of native and monomethoxypolyethylene glycol-modified superoxide dismutase isolated from bovine liver, as well as native and aldehyde dextran. The study demonstrated that the biodistribution of the native enzymes from various sources was, however, equal, but in the mouse liver there was a higher accumulation of SOD isolated from the rat liver. AD-SOD was found to have a longer half-life in the blood and in the liver of mice, in particular, while MPEG-SOD showed 10, 15, and 16 times longer in the lungs, blood and heart of the animals examined, respectively. The elevated accumulation of MPEG-SOD in some organs was used for their treatment, particularly for experimental therapy of rat myocardial ischemia. A rat model of ischemia demonstrated that the intravenous bolus administration of MPEG-SOD reduced the size of a myocardial necrotic area by 40% as compared to a 13% decrease when the other compounds were assayed. The findings suggest that the MPEG-SOD preparation is promising for decreasing reperfusion injuries of the cardiovascular system and the lungs.
Maksimenko
A V
AV
Institute of Experimental Cardiology, Cardiology Research Center, Russian Academy of Medical Sciences, Moscow.
Petrov
A D
AD
Caliceti
P
P
Konovalova
G G
GG
Schiavon
O
O
Grigor'eva
E L
EL
Lankin
V Z
VZ
Veronese
F M
FM
D000818:Animals; D002417:Cattle; D004353:Drug Evaluation, Preclinical; D007457:Iodine Radioisotopes; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D017202:Myocardial Ischemia / Q000188:drug therapy*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D013482:Superoxide Dismutase / Q000187:drug effects* / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000627:therapeutic use*; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D013482:Superoxide Dismutase
Modifikatsiia Cu, Zn-superoksiddismutazy monometoksipoliétilenglikolem uluchshaet pokazateli ékpserimental'noĭ terapii ishemizirovannogo miokarda krys.