[Study on determination of gentamycin by enhanced chemiluminescent immunoassay].
Gentamycin was determined by solid antigen competition inhibition method of chemiluminescet immunoassay, in which luminol-(p-hydroxydiphenyl)-H2O2 was used a the chemiluminescence system and p-hydroxydiphenyl as the chemiluminescent enhancer. Solid antigen was synthesized by a method in which gentamycin was immobilized covalently on microcrystalline cellulose previously activated by 1,1'-carbonyldiimidazole. The rabbit anti-gentamycin IgG and the HRP-donkey anti-rabbit IgG were used as the first antibody and labelled second antibody, respectively. The linear range of the gentamycin (with blank serum) was 0.03-1 micrograms/tube and the correlation coefficient of the log-log regression equation [r[ was 0.981 +/- 0.016 with a limit of detection 3.3-11.4 ng/tube and the recovery of gentamycin 88.2 +/- 4.5%.
Deng
A
A
Yang
X
X
Yang
Y
Y
Yang
H
H
D001713:Biphenyl Compounds; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D007093:Imidazoles; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements; D008165:Luminol
D001713:Biphenyl Compounds; D005839:Gentamicins; D007093:Imidazoles; D008165:Luminol; C006900:N,N-carbonyldiimidazole; C019046:4-phenylphenol