title: The effect of the addition of albumin to cardioplegic and preservation solutions on the isolated rat heart.
abstract: This experimental study evaluates the effect of the addition of albumin to cardioplegic and preservation solutions on isolated rat hearts. Male Wistar rat hearts were arrested with a 4 degrees C crystalloid cardioplegic solution. All hearts were immersed for 8 hours in a 4 degrees C Euro-Collins solution. They were divided into four groups: group I, the cardioplegic solution was used for cardiac arrest, and the 4 degrees C Euro-Collins solution did not contain albumin; group II, the cardioplegic solution was adjusted to an albumin concentration of 3%; group III, the preservation solution contained 3% albumin; and group IV, both the cardioplegic and the preservation solutions contained 3% albumin. The cardiac output for group IV increased more than that for any other group (p < 0.05 or p < 0.01). The total concentration of creatine kinase-MB at 30 minutes after reperfusion in group III decreased less than that in groups I and II. The level of myocardial adenosine triphosphate in group IV was better preserved than that in any other group; the myocardial calcium content in group IV decreased compared to the other groups. However, no significant difference between groups III and IV was found. The level of malondialdehyde as a measure of lipoperoxide values showed significant differences between groups I and IV and groups II and IV at 5 minutes after reperfusion (p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Second Department of Surgery, Kurume University School of Medicine, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hisatomi
authors.forename: T
authors.initials: T
authors.lastname: Isomura
authors.forename: M
authors.initials: M
authors.lastname: Yomoda
authors.forename: K
authors.initials: K
authors.lastname: Ohishi
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000418:Albumins; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002302:Cardiac Output; D003402:Creatine Kinase / Q000032:analysis; D006321:Heart / Q000502:physiology*; D006339:Heart Rate; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D007527:Isoenzymes; D007773:Lactates / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation; D011188:Potassium; D017680:Potassium Compounds; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000418:Albumins; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007527:Isoenzymes; D007773:Lactates; D017680:Potassium Compounds; C018369:potassium cardioplegic solution; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D011188:Potassium; D002118:Calcium