title: [The quantitative assay of (1-->3)-beta-D-glucan in culture medium of Candida albicans using G-test].
abstract: It has been difficult to diagnose the deep-seated fungal infection. Limulus test which originally has been developed to detect endotoxin in blood is also activated by (1-->3)-beta-D-glucan, the cell wall component of the fungi. Factor G in limulus lysate is activated by (1-->3)-beta-D-glucan and not by endotoxin. The quantitative assay of (1-->3)-beta-D-glucan is possible by the G-test using factor G. (1-->3)-beta-D-glucan in RPMI culture medium of Candida albicans was periodically measured using G-test and the effect of antifungal drug or neutrophil to the changes of (1-->3)-beta-D-glucan in the culture medium was studied. Increase in the level of (1-->3)-beta-D-glucan was in parallel with the growth of Candida albicans. G-test may be applied to the clinical diagnosis of fungal infection.
authors.affiliation: Department of Surgery II, Nagoya University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: A
authors.initials: A
authors.lastname: Nakao
authors.forename: T
authors.initials: T
authors.lastname: Kambe
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Tamura
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Takagi
mesh_terms: D002176:Candida albicans / Q000254:growth & development*; D002177:Candidiasis / Q000175:diagnosis; D003470:Culture Media / Q000032:analysis*; D005936:Glucans / Q000032:analysis*; D006801:Humans; D008033:Limulus Test / Q000379:methods*; D047071:beta-Glucans
chemical_list: D003470:Culture Media; D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan