O-linked neutral sugar chains of porcine zona pellucida glycoproteins.
The O-linked sugar chains were liberated from porcine zona pellucida glycoproteins by alkaline-borohydride treatment and the structures of neutral oligosaccharides were determined. The major oligosaccharides had the following structures with type-1 cores, (Gal beta 1-->4GlcNAc beta 1-->3)1 3-Gal bdta 1-->3N-acetylgalactosaminitol (54%) and GlcNAc beta 1-->3(Gal beta 1-->4GlcNAc beta 1-->3)0-1Gal beta 1-->3N- acetylgalactosaminitol (16%). Approximately 6% of the oligosaccharides had the structure with a type-3 core, Gal beta 1-->4GlcNAc beta 1-->3N-acetylgalactosaminitol. Oligosaccharides having alpha-galactose (2%) and beta-GalNAc (4%) at their non-reducing termini were also found as minor components.
Hirano
T
T
Department of Biochemistry, University of Tokyo, Japan.
Takasaki
S
S
Hedrick
J L
JL
Wardrip
N J
NJ
Amano
J
J
Kobata
A
A
D000818:Animals; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000737:chemistry*; D037102:Lectins; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D037121:Plant Lectins; D034781:Receptors, N-Acetylglucosamine; D013552:Swine; D015044:Zona Pellucida / Q000737:chemistry*
D006023:Glycoproteins; C045475:Griffonia simplicifolia lectins; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; D034781:Receptors, N-Acetylglucosamine; C017707:Ricinus communis agglutinin-1; C040129:wisteria lectin