title: An advanced solid support for immunoassays and other affinity applications.
abstract: A 6-mm diameter glass bead that has a hydrophilic coating containing epoxy groups has been developed for immunoassays and other affinity applications. These QuantAffinity beads covalently bind protein in the range of 200-400 ng per bead. Derivatization with protein in the presence of high concentrations of potassium phosphate (pH 7.0-8.0) generally gives complete coupling in 16 hours. No preactivation of the support or the protein is necessary. Low nonspecific binding and high retention of bound protein activity are inherent in the system due to the special surface chemistry. Several enzymatic and radioactive immunoassays as well as a biotin-avidin-based assay are described. A use of QuantAffinity beads in small-scale purification is also presented.
authors.affiliation: Terrapin Technologies, South San Francisco, CA 94080.
authors.forename: D E
authors.initials: DE
authors.lastname: Schmidt
authors.forename: T L
authors.initials: TL
authors.lastname: Brooks
authors.forename: S
authors.initials: S
authors.lastname: Mhatre
authors.forename: R P
authors.initials: RP
authors.lastname: Junghans
authors.forename: M B
authors.initials: MB
authors.lastname: Khazaeli
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D006735:Horseradish Peroxidase / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008863:Microspheres; D011506:Proteins / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; C051538:IGG-horseradish peroxidase; D007074:Immunoglobulin G; D011506:Proteins; D015375:Receptors, Interleukin-2; D006735:Horseradish Peroxidase