Isolation and characterization of serologically reactive lipooligosaccharides from Mycobacterium tuberculosis.
Two major highly polar antigenic glycolipids were isolated from recent isolates of Mycobacterium tuberculosis from a wide range of geographical origins. The occurrence of these polar glycolipids was demonstrated by isolation, purification and chromatographic characterization and/or serological procedures in 12 strains. Based on their chromatographic properties, these polar glycolipids belong to the lipooligosaccharide family. Preliminary data on the use of these newly described antigens in the serodiagnosis of tuberculosis is presented.
Papa
F
F
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris, France.
Cruaud
P
P
Luquin
M
M
Thorel
M F
MF
Goh
K S
KS
David
H L
HL
D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D066298:In Vitro Techniques; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D012698:Serologic Tests; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology
D006017:Glycolipids; D007074:Immunoglobulin G