Stimulation of pancreatic growth by cholecystokinin is mediated by high affinity receptors on rat pancreatic acinar cells.
Pancreatic acinar cells possess both high low affinity receptors for cholecystokinin. The cholecystokinin analog caerulein, which exerts a trophic effect on the rat pancreas, acts as an agonist at both types of receptors. In contrast, the synthetic analog CCK-JMV-180, which also acts as an agonist at high affinity receptors, opposes the action of caerulein on the low affinity receptors. We report that infusion of either caerulein or CCK-JMV-180 into rats increases [3H]-thymidine incorporation into pancreatic DNA and causes the pancreatic weight as well as content of DNA, RNA, and protein to increase. CCK-JMV-180 also stimulates in-vitro incorporation of [3H]-thymidine into DNA of cultured rat acini. The finding that both caerulein and CCK-JMV-180 exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell cholecystokinin receptors.
Dawra
R
R
Department of Surgery, Beth Israel Hospital/Harvard Medical School, Boston, MA 02215.
Saluja
A
A
Lerch
M M
MM
Saluja
M
M
Logsdon
C
C
Steer
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002108:Ceruletide / Q000494:pharmacology; D002766:Cholecystokinin / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D007700:Kinetics; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000187:drug effects* / Q000378:metabolism; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D011949:Receptors, Cholecystokinin; D014316:Tritium; C058451:JMV 180; D002108:Ceruletide; D004247:DNA; D002766:Cholecystokinin; D012844:Sincalide; D013936:Thymidine