A rapid and simple method for reversing the inhibitory effect of heparin on PCR for HLA class II typing.
Poli
F
F
Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico, Milan, Italy.
Cattaneo
R
R
Crespiatico
L
L
Nocco
A
A
Sirchia
G
G
D002614:Chelating Agents; D004247:DNA / Q000097:blood / Q000235:genetics; D005069:Evaluation Studies as Topic; D005784:Gene Amplification; D005802:Genes, MHC Class II; D006493:Heparin / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D019384:Nucleic Acid Synthesis Inhibitors; D016133:Polymerase Chain Reaction / Q000379:methods*; D012117:Resins, Synthetic; D019914:Taq Polymerase
D002614:Chelating Agents; D019384:Nucleic Acid Synthesis Inhibitors; D012117:Resins, Synthetic; C024997:Chelex 100; D006493:Heparin; D004247:DNA; D019914:Taq Polymerase