Effects of starvation on the urinary contents of primary thromboxane and prostacyclin metabolites and a possible selenium-dependent role of prostacyclin in the renal handling of ketone bodies.
The aim of this study was to investigate whether urinary prostanoids, as an index of renal synthesis of these compounds, are affected in selenium (Se) deficiency and, if so, whether such changes could add to our understanding of the high excretion of ketone bodies in Se-deficient rats (p < 0.005 vs Se-adequate rats). Male rats were fed a Se-deficient diet with less than 0.01 mg Se/kg or the same diet supplemented with 0.2 mg Se/kg. The urinary contents of prostaglandin E2 (PGE2), PGF2 alpha and 6-keto PGF1 alpha were not significantly affected by the Se status. However, there was a positive correlation between the urinary contents of ketone bodies and 6-keto PGF1 alpha in Se deficiency (with p < 0.02 for acetaoacetate and p < 0.05 for 3-hydroxybutyrate). In contrast, only negative (nonsignificant) relationships were observed between these same parameters in Se-adequate rats. No correlations between urinary contents of ketone bodies and PGE2, PGF2 alpha or thromboxane B2 (TXB2) were obtained. Compared to fed rats, starvation caused a 4-fold increase in the urinary TXB2 content in Se-adequate, as well as in Se-deficient rats (p < 0.001). Starvation had an opposite effect on the content of 6-keto PGF1 alpha, which decreased (to 64% that of fed animals p < 0.001) in Se-adequate rats and, nonsignificantly (to 93% that of fed animals) in the Se-deficient group. It is concluded that starvation profoundly affects the urinary contents (and thus, probably renal synthesis) of TX and prostacycline (PGI2).(ABSTRACT TRUNCATED AT 250 WORDS)
Olsson
U
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Department of Genetic and Cellular Toxicology, Wallenberg Laboratory, Stockholm University, Sweden.
D015121:6-Ketoprostaglandin F1 alpha / Q000652:urine; D000818:Animals; D015237:Dinoprost / Q000652:urine; D015232:Dinoprostone / Q000652:urine; D007657:Ketone Bodies / Q000652:urine*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000378:metabolism / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000172:deficiency*; D013929:Thromboxane B2 / Q000652:urine
D007657:Ketone Bodies; D011453:Prostaglandins; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D012643:Selenium; D015232:Dinoprostone