title: Enhanced mRNA content in response to amino acid starvation for a 73 kDa protein of the inner mitochondrial membrane.
abstract: Biosynthesis of several rat liver proteins is enhanced by amino acid deprivation of cultured hepatocytes or hepatoma cells. One of these proteins, MP-73, was synthesized at a rate 2- to 3-fold greater when cells were incubated for 3-9 h under conditions of amino acid deprivation versus amino acid supplementation. Immunoblotting with polyclonal antibodies prepared against MP-73 localized it to the inner mitochondrial membrane. MP-73 appears to be a hydrophobic, integral membrane protein. MP-73 antibody was used to identify a partial cDNA (NS3.2) of approximately 2 kb. A probe prepared from pNS3.2 identified a transcript in rat Fao hepatoma cells of approximately 4.4 kb that was increased in abundance by more than 20-fold following amino acid starvation of the cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville 32610.
authors.forename: T C
authors.initials: TC
authors.lastname: Chiles
authors.forename: R O
authors.initials: RO
authors.lastname: Laine
authors.forename: N F
authors.initials: NF
authors.lastname: Shay
authors.forename: M E
authors.initials: ME
authors.lastname: Handlogten
authors.forename: H S
authors.initials: HS
authors.lastname: Nick
authors.forename: M S
authors.initials: MS
authors.lastname: Kilberg
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA; D015870:Gene Expression; D015723:Gene Library; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008930:Mitochondria, Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
chemical_list: D000596:Amino Acids; D008565:Membrane Proteins; D012333:RNA, Messenger; C082025:mitochondrial membrane protein 73; D004247:DNA