title: Rat pancreas kinesin: identification and potential binding to microtubules.
abstract: We have demonstrated the presence of kinesin in the secretory pancreatic tissue using SDS-PAGE, immunoblot and immunoelectron microscopy techniques. Polyclonal antibodies were raised against the rat brain kinesin heavy chain and affinity-purified. Immunoblot studies showed that these antibodies were bound to a 116 kDa protein found in rat pancreas crude extracts and in partially purified kinesin fractions. Kinesin identification was also performed by a cosedimentation procedure based on its strong binding to microtubules in the presence of sodium fluoride. The microtubule-kinesin complex was observed by immunoelectron microscopy gold staining. The reversible association of kinesin with microtubules was generated by MgATP.
authors.affiliation: Unité de Biologie Cellulaire et Physiopathologie Digestives, INSERM U.61, Strasbourg, France.
authors.forename: K
authors.initials: K
authors.lastname: Malekzadeh-Hemmat
authors.forename: P
authors.initials: P
authors.lastname: Gendry
authors.forename: J F
authors.initials: JF
authors.lastname: Launay
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D001923:Brain Chemistry; D015151:Immunoblotting; D016547:Kinesins / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000255:Adenosine Triphosphate; D016547:Kinesins