[Measurement of serum concentration with radioimmunoassay for carboxyterminal propeptide of type 1 procollagen].
A radioimmunoassay kit for measurement of carboxyterminal propeptide of type 1 procollagen (P1CP) was developed and can be purchased commercially for clinical use. Using the kit, we measured serum concentration in healthy controls and in patients with bone metastasis and other various skeletal disorders. In healthy controls, serum concentration of P1CP ranged within 37-177 ng/ml under age 50, while in serum concentration of women over 50, it elevated upto 350 ng/ml. In patients with skeletal metastasis, in most of patients, it stayed within a normal range, whereas in patients with bone metastasis from prostatic cancer, it raised significantly. In some of patients with primary hyperparathyroidism or hyperthyroidism, serum concentration for P1CP was also elevated. In comparison with other serum bone metabolic markers such as osteocalcin or alkaline phosphatase, P1CP showed less occurrence of an elevation in patients with non-skeletal disease. Serum concentration of P1CP was not affected by renal function, while mild elevation was observed in patients with severely damaged liver diseases. In conclusion, the newly developed radioimmunoassay for P1CP was an excellent assay system and would provide us easily evaluation of type 1 collagen formation.
Yamamoto
I
I
Department of Radiology, Shiga University of Medical Science.
Takada
M
M
Ohnaka
Y
Y
Yuu
I
I
Yamamoto
Y
Y
Hamazu
H
H
Morita
R
R
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D001859:Bone Neoplasms / Q000175:diagnosis / Q000556:secondary; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood*; D011347:Procollagen / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic / Q000592:standards
D015415:Biomarkers; D010446:Peptide Fragments; D011347:Procollagen; D011933:Reagent Kits, Diagnostic; C042813:procollagen type I carboxy terminal peptide