title: Experimental transmission of Dipetalonema dracunculoides (Cobbold 1870) by Rhipicephalus sanguineus (Latreille 1806).
abstract: A dog naturally infected with Dipetalonema dracunculoides and having a microfilaremia of 6050 microfilariae per mm3 of blood was used as source of infection. Experimentally cultivated nymphs of Rhipicephalus sanguineus were fed on the donor dog. Once engorged, ninety-three nymphs were removed and kept at 30 degrees C and RH 90% until they moulted to the adult stage. To study the development of microfilariae in the vector, ten ticks were dissected at Day 37 post-infection. Adult infected ticks were fed on two uninfected dogs. The observed pre-patent periods were 69 and 76 days. Trans-stadial transmission of Dipetalonema dracunculoides by Rhipicephalus sanguineus was demonstrated.
authors.affiliation: Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: A S
authors.initials: AS
authors.lastname: Olmeda-García
authors.forename: J A
authors.initials: JA
authors.lastname: Rodríguez-Rodríguez
authors.forename: F A
authors.initials: FA
authors.lastname: Rojo-Vázquez
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology*; D004153:Dipetalonema / Q000254:growth & development*; D004154:Dipetalonema Infections / Q000635:transmission / Q000662:veterinary*; D004283:Dog Diseases / Q000635:transmission*; D004285:Dogs; D005260:Female; D008297:Male; D008842:Microfilariae / Q000254:growth & development; D009758:Nymph / Q000469:parasitology; D013047:Specific Pathogen-Free Organisms; D013987:Ticks / Q000469:parasitology*