Recombinant human IL-5 augments immunoglobulin generation by human B lymphocytes in the presence of IL-2.
The biological activity of interleukin-5 (IL-5) on human B lymphocytes was examined by using a newly purified recombinant human IL-5 preparation. Different densities of B cells from human tonsil samples were isolated by Percoll density gradient from nonrosetted cells. The IL-5 preparation did not act as a B cell growth factor on the large (in vivo-activated) or small (resting) B cells after activation with polyclonal B cell activators. The IL-5 augmented the immunoglobulin (Ig) generation of Staphylococcus aureus Cowan strain I (SAC)-activated B blasts in the presence of interleukin-2 (IL-2). The effect of IL-5 on Ig generation was greater in pokeweed mitogen (PWM)-driven B blasts than that in SAC-induced B blasts when assessed by enzyme-linked immunosorbent assay. The amounts of mu RNA isolated from IL-5/PWM-treated cells were also larger than those from IL-5/IL-2-treated B blasts. These results suggest that the human IL-5 can increase Ig generation of B cells in the presence of IL-2 by working at the level of mRNA coding for Ig.
Morikawa
K
K
Department of Internal Medicine, Shimane Medical University, Japan.
Oseko
F
F
Morikawa
S
S
Imai
K
K
Sawada
M
M
D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000276:immunology*; D015848:Interleukin-5 / Q000494:pharmacology*; D014066:Palatine Tonsil / Q000276:immunology; D011043:Pokeweed Mitogens; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013211:Staphylococcus aureus; D013997:Time Factors
D007136:Immunoglobulins; D007376:Interleukin-2; D015848:Interleukin-5; D011043:Pokeweed Mitogens; D012333:RNA, Messenger; D011994:Recombinant Proteins