A chromosome 11 YAC library.
A targeted yeast artificial chromosome (YAC) library for chromosome 11 has been constructed from the J1 cell line that carries a single human chromosome 11 within a hamster DNA background. Interspecies chimeric clones generated during construction of the library were detected during the screening process and eliminated from the library. Contig assembly becomes much less difficult using such a library as the complexity is decreased and the ends of the clone inserts can be rescued for walking to neighboring clones. The library contains > 1824 clones with an average insert length of 337 kb. This represents a fourfold coverage of chromosome 11 or a > 95% chance of recovering a unique single-copy sequence from the library. Two hundred YAC clones were localized by fluorescence in situ hybridization and found to be randomly distributed along the chromosome. The library has been screened with probes for the chromosome 11 markers HBB, GLUR4, H19, and D11S193. Corresponding YAC clones have been isolated for each locus. This analysis has indicated that the library is unbiased, that cognate YAC clones can be recovered with chromosome 11 markers, and that extensive contig assembly should be feasible.
Qin
S
S
Department of Human Genetics, Roswell Park Cancer Institute, New York State Department of Health, Buffalo 14263.
Zhang
J
J
Isaacs
C M
CM
Nagafuchi
S
S
Jani Sait
S N
SN
Abel
K J
KJ
Higgins
M J
MJ
Nowak
N J
NJ
Shows
T B
TB
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002871:Chromosome Banding; D015825:Chromosomes, Fungal; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D006224:Cricetinae; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D017403:In Situ Hybridization