Electro-immortalization of B lymphocytes isolated from stomach carcinoma biopsy material.
The advantages of electrofusion were used to immortalize the small number of B-cells from fresh biopsy material taken from a gastric carcinoma of a patient. Two stable human antibody secreting clones could be produced which exhibited functional activity against the autologous tumour cells (inhibition of cell adhesion and immunofluorescence staining of the membranes). This shows that a variety of hitherto inaccessible B lymphocyte populations from other human organ biopsies can be immortalised by the improved electrofusion technique.
Vollmers
H P
HP
Institut für Pathologie, Universität Würzburg, Germany.
von Landenberg
P
P
Dämmrich
J
J
Stulle
K
K
Wozniak
E
E
Ringdörfer
C
C
Müller-Hermelink
H K
HK
Herrmann
B
B
Zimmermann
U
U
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology*; D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000912:Antibodies, Neoplasm / Q000276:immunology / Q000302:isolation & purification*; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D001706:Biopsy; D002448:Cell Adhesion; D002459:Cell Fusion; D004560:Electricity; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D008297:Male; D051379:Mice; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm