title: Trichuris chilensis: physicochemical and immunological characterization of the different antigenic components.
abstract: The protein pattern of Trichuris chilensis obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was analyzed. Complex protein band patterns covering a wide range of molecular weights were obtained. The molecular weights of the major proteins present in different tissue homogenates were estimated. Antisera raised in rabbits against homogenates of T. chilensis and sera from naturally infected Ctenomys australis were used in Western blotting, immunoelectrophoresis and passive hemagglutination to compare the antigenicity of the adult male, adult female, eggs, oocytes, stichosome and cuticle of this parasite. Specific antibodies to parasite antigens were also detected in faecal preparations and caecum mucosal extracts of C. australis naturally infected with T. chilensis.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
authors.forename: C A
authors.initials: CA
authors.lastname: Pujol
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D005260:Female; D015801:Helminth Proteins / Q000032:analysis* / Q000276:immunology; D008297:Male; D011817:Rabbits; D012377:Rodentia / Q000276:immunology / Q000469:parasitology; D014258:Trichuris / Q000737:chemistry* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins