title: Polyclonal enzyme immunoassay method for detection of motile and non-motile Salmonella in foods: comparative study.
abstract: A new enzyme immunoassay (EIA) method for detection of motile and non-motile Salmonella was examined in a comparative study. This method uses a proprietary formulation of polyclonal antibodies to Salmonella and is controlled to maintain specificity. Sensitivity is enhanced with an additional antibody reaction designed to minimize false-negative reactions attributable to steric interference that can occur during conjugate binding in immunoassay procedures. Twenty food types representative of a wide variety of food products were analyzed by both the EIA method and the AOAC/Bacteriological Analytical Manual (BAM) method, 967.26. Of the 1000 samples analyzed, there was a 95.6% agreement rate between the EIA method and the AOAC/BAM method. False-negative rates for the 2 methods were comparable for all foods and all Salmonella levels except ground poultry, where the EIA method detected significantly more confirmed positive samples than did the AOAC/BAM method. Twenty-seven samples were positive by EIA but negative by the culture method, and 17 samples were negative by EIA but positive by the culture method. There were no false-positive isolates detected in the comparative study.
authors.affiliation: BioControl Systems, Inc., Bothell, WA 98011.
authors.forename: P T
authors.initials: PT
authors.lastname: Feldsine
authors.forename: M T
authors.initials: MT
authors.lastname: Falbo-Nelson
authors.forename: D L
authors.initials: DL
authors.lastname: Hustead
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001688:Biological Products; D001842:Bone and Bones / Q000737:chemistry; D002417:Cattle; D002645:Chickens; D003470:Culture Media; D033364:Decapoda; D005399:Fishes; D005516:Food Microbiology; D007124:Immunoenzyme Techniques; D008460:Meat / Q000032:analysis; D008892:Milk / Q000382:microbiology; D008903:Minerals / Q000032:analysis; D009754:Nuts / Q000737:chemistry; D012475:Salmonella / Q000737:chemistry*; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial; D001688:Biological Products; D003470:Culture Media; D008903:Minerals; C027762:bone meal