[Clinical and experimental studies on the cellular kinetics in esophageal carcinoma by using monoclonal antibody KI-67].
As subjects, 130 patients with thoracic esophageal carcinoma and 48 BALB/c nude mice bearing human esophageal carcinoma were used in this study. Fresh frozen specimens of the tumors were stained by the indirect immunohistochemical technique using monoclonal antibody Ki-67. Cancerous cell inside of whose nuclear was stained were judged as Ki-67 positive cells and Ki-67 positive cell rate was calculated by measuring the positive cell counts per 2,000 tumorous cells. Studies was made regarding the relationship between Ki-67 positive cells and DNA content in cancerous cell nucleus, emergence of epidermal growth factor receptor, degree of infiltration of Leu-7 cell around the tumor and postoperative death of recurrence. There were no significant relationship between Ki-67 positive rate and histologic prognosis factors. However, a significant difference of the postoperative death was found between the cases with and without Ki-67 positive cells. By the experimental study, no constant relation was observed between the growth and the proliferation of tumor. These results suggest that the carcinoma with Ki-67 positive cells can be a malignancy index for esophageal carcinoma for its high death of recurrence, although it seems to be difficult to grasp the certain proliferation kinetics by the results of measurement at a time point.
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First Department of Surgery, Kurume University School of Medicine, Japan.
D000818:Animals; D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000473:pathology*; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000276:immunology*; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000276:immunology*; D011379:Prognosis
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins