A simple micromanipulator for multiple uses in freely moving rats: electrophysiology, voltammetry, and simultaneous intracerebral infusions.
An inexpensive, easily fabricated micromanipulator is described that can be used for single-unit recording or voltammetry in freely moving rats. The basic design is configured around the standard coupling system between a plastic syringe and corresponding needle hub. The device can be used with glass or metal microelectrodes for electrophysiology or carbon-fiber or carbon-disk microelectrodes for voltammetry. With either recording technique, the micromanipulator also can accommodate a 33-ga infusion cannula, which allows drugs to be administered directly to the recording site. The entire assembly is lightweight and can be used with a head-mounted amplifier system for relatively noise-free recording.
Rebec
G V
GV
Department of Psychology, Indiana University, Bloomington 47405.
Langley
P E
PE
Pierce
R C
RC
Wang
Z
Z
Heidenreich
B A
BA
D000200:Action Potentials; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D004567:Electrodes, Implanted; D004594:Electrophysiology / Q000295:instrumentation*; D007267:Injections / Q000295:instrumentation*; D008839:Microelectrodes; D008846:Micromanipulation / Q000295:instrumentation*; D051381:Rats; D013238:Stereotaxic Techniques; D013594:Syringes