title: A liquid chromatographic procedure for the analysis of yohimbine in equine serum and urine.
abstract: A standardbred mare was dosed with 40 mg yohimbine intravenously. Serum and urine samples were collected and analyzed for yohimbine using solvent extraction and reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection. Maximum yohimbine concentrations of 45 and 18 ng/mL were observed in serum and urine samples, respectively. Elimination was rapid, with half-lives of approximately 20 and 53 min observed for serum and urine, respectively. The presence of yohimbine in these samples was confirmed by liquid chromatography/mass spectroscopy (LC/MS/MS).
authors.affiliation: Can Test Ltd., Vancouver, B.C.
authors.forename: G
authors.initials: G
authors.lastname: Reimer
authors.forename: A
authors.initials: A
authors.lastname: Suarez
authors.forename: Y C
authors.initials: YC
authors.lastname: Chui
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005260:Female; D006207:Half-Life; D006736:Horses; D013058:Mass Spectrometry; D013050:Spectrometry, Fluorescence; D015016:Yohimbine / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D015016:Yohimbine