title: Rapid detection of infectious mononucleosis-associated heterophile antibodies by a novel immunochromatographic assay and a latex agglutination test.
abstract: A novel immunochromatographic assay, the CARDS O.S. MONO test (Pacific Biotech, San Diego, Calif.), and a latex agglutination test, the Infectious Mononucleosis Kit (Unipath Ltd., Hampshire, United Kingdom) were compared with the Paul-Bunnell-Davidsohn test. Of the 957 serum specimens studied, 78 were positive and 879 were negative by the Paul-Bunnell-Davidsohn test. After discrepancies were resolved by determining Epstein-Barr virus serology, the sensitivities of the CARDS O.S. MONO test and the Infectious Mononucleosis Kit were 91.0 and 96.2%, respectively, and both tests had a specificity and a positive predictive value of 100% and a negative predictive value and overall agreement of greater than 99%. The results show that both tests can accurately detect infectious mononucleosis-associated heterophile antibodies.
authors.affiliation: Department of Microbiology, Daniel Medical Laboratories, Downsview, Ontario, Canada.
authors.forename: S E
authors.initials: SE
authors.lastname: Farhat
authors.forename: S
authors.initials: S
authors.lastname: Finn
authors.forename: R
authors.initials: R
authors.lastname: Chua
authors.forename: B
authors.initials: B
authors.lastname: Smith
authors.forename: A E
authors.initials: AE
authors.lastname: Simor
authors.forename: P
authors.initials: P
authors.lastname: George
authors.forename: B B
authors.initials: BB
authors.lastname: Diena
authors.forename: D
authors.initials: D
authors.lastname: Diena
authors.forename: M
authors.initials: M
authors.lastname: Skulnick
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000910:Antibodies, Heterophile / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002845:Chromatography; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000706:statistics & numerical data; D007244:Infectious Mononucleosis / Q000175:diagnosis* / Q000276:immunology; D007841:Latex Fixation Tests / Q000706:statistics & numerical data; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000910:Antibodies, Heterophile