title: Cell surface binding of colloidal iron and Limax flavus agglutinin compared by X-ray microanalysis.
abstract: The surface characteristics of tumour cells and T-cell hybridoma variants were examined by quantitative X-ray microanalysis using metal-based probes. Comparisons of populations of cells differing in invasive potential revealed differences in the binding of positive colloidal iron hydroxide, a general probe for anionic sites. Substantial differences between populations were also detected by the binding of Limax flavus agglutinin, which was used in conjunction with fetuin-gold as a specific marker for sialic acid.
authors.affiliation: Department of Biological Sciences, University of Wales, Dyfed, U.K.
authors.forename: N
authors.initials: N
authors.lastname: Thomas
authors.forename: P M
authors.initials: PM
authors.lastname: Evans
authors.forename: I
authors.initials: I
authors.lastname: ap Gwynn
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism*; D003102:Colloids; D004577:Electron Probe Microanalysis; D007501:Iron / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D019158:N-Acetylneuraminic Acid; D009361:Neoplasm Invasiveness; D037121:Plant Lectins; D011485:Protein Binding; D012794:Sialic Acids / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D000838:Anions; D003102:Colloids; D037102:Lectins; C042962:Limax flavus agglutinin; D037121:Plant Lectins; D012794:Sialic Acids; D007501:Iron; D019158:N-Acetylneuraminic Acid