The role of the beta-subunit of initiation factor eIF-2 in initiation complex formation.
The functional properties of preparations of protein synthesis initiation factor eIF-2 which lack the beta-subunit (as confirmed immunologically) were compared with those of the heterotrimeric factor. The former can bind guanine nucleotides but not initiator tRNA, and also exhibits a substantially reduced rate of initiation factor eIF-2B-mediated GDP/GTP-exchange.
Flynn
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A
Department of Biochemistry, School of Medical Sciences, University of Bristol, UK.
Oldfield
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S
Proud
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CG
D000818:Animals; D015852:Eukaryotic Initiation Factor-2 / Q000737:chemistry / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors; D006153:Guanosine Diphosphate / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012358:RNA, Transfer, Met / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D015852:Eukaryotic Initiation Factor-2; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D012358:RNA, Transfer, Met; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate