Human cDNA encoding DnaJ protein homologue.
We have cloned a cDNA encoding a DnaJ-like protein from the human fibrosarcoma HT-1080 cDNA library. This cDNA encodes a protein of 397 amino acid residues whose sequence shows 38.2% identity with the Escherichia coli DnaJ protein and 47.2% with the yeast DnaJ homologue along the entire length. Since the sequence contains the N-terminal domain region conserved in DnaJ family members and the four repeats of the Cys-X-X-Cys-X-Gly-X-Gly motif which are characteristic of DnaJ proteins, we conclude that this cDNA clone encodes the human homologue of DnaJ.
Oh
S
S
Sagami Chemical Research Center, Kanagawa, Japan.
Iwahori
A
A
Kato
S
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000235:genetics; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured
D001426:Bacterial Proteins; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; C070319:YDJ1 protein, S cerevisiae