Lymphomagenesis in AKR.Fv-1b congenic mice.
In the AKR.Fv-1b congenic strain the Fv-1n allele of the AKR/J mice was substituted with the Fv-1b allele, thereby limiting viral replication and spread of the endogenous N-tropic murine leukemia virus. As a result of this genetic change AKR.Fv-1b mice develop a low spontaneous incidence (7%) of T-cell lymphomas and about 28% of Ly-1+ B-cell lymphomas are observed in old mice. Characteristic changes in thymus subpopulations of AKR/J mice (related to the formation of the dual tropic mink cell focus inducing (MCF) type virus in the thymus) were not observed in the thymus of AKR.Fv-1b mice. In contrast to the low susceptibility to spontaneous T-cell lymphoma development, these mice were highly sensitive to fractionated irradiation or to radiation leukemia virus (a mixture of N- and B-tropic viruses) induced T-cell lymphoma. Potential lymphoma cells (that would ultimately develop into Ly-1+ B-cell lymphomas) were demonstrated in bone marrow and spleens of 16-24-month-old mice. Analysis of the Ly-1+ IgM+ B-cell population in spleens of 18-month-old mice revealed a significant increase in this population (35% versus 2% in young spleens). The spontaneous Ly-1+ B-cell lymphoma incidence could be enhanced (up to 77%) by in vivo administration of anti-CD8 monoclonal antibody or IL-4 to 18-month-old mice. Virological analysis of T/B-cell lymphomas for class I MCF viruses indicated that Class I MCF development was tightly correlated with T-lymphoma development (except radiation induced tumors that showed no MCF provirus involvement). In contrast, Ly-1+ B-cell lymphoma development was independent of Class I MCF pathogenic virus involvement.
Haran-Ghera
N
N
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Peled
A
A
Brightman
B K
BK
Fan
H
H
D000406:AKR murine leukemia virus / Q000235:genetics / Q000276:immunology; D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D005260:Female; D005814:Genes, Viral; D016393:Lymphoma, B-Cell / Q000209:etiology* / Q000276:immunology / Q000382:microbiology; D016399:Lymphoma, T-Cell / Q000209:etiology* / Q000276:immunology / Q000382:microbiology; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR / Q000235:genetics; D013953:Thymus Neoplasms / Q000209:etiology* / Q000276:immunology / Q000382:microbiology
D000943:Antigens, Differentiation