Synthesis and use of an internal amino acid sequencing standard peptide.
A sequenceable internal standard has been made that can be added to samples destined for automated amino acid sequencing and that provides a means for continuously monitoring instrument performance. The 41-residue standard peptide is composed of two unnatural amino acids, norleucine and succinyl-lysine, and has the structure [norleucine-(succinyl-lysine)4]8-norleucine. The phenylthiohydantoin derivatives of the latter two amino acids are well separated via reversed-phase HPLC from the phenylthiohydantoin derivatives of the 20 naturally occurring amino acids. In addition, the standard peptide can be readily synthesized and it has excellent solubility, stability, and sequencing characteristics. The placement of norleucine at every fifth residue in this peptide permits accurate repetitive yield and carryover calculations following runs that are as short as six cycles. Use of this internal standard permits the early detection of suboptimal instrument performance and is especially helpful in differentiating between two common reasons (blocked NH2-terminus versus an instrument problem) for the failure of an automated protein sequencer to provide an NH2-terminal sequence.
Elliott
J I
JI
W.M. Keck Foundation Biotechnology Resource Laboratory, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
Stone
K L
KL
Williams
K R
KR
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012015:Reference Standards
D010455:Peptides; C081994:(norleucyl-(succinyllysyl)4)(8)-norleucine