The main allergen of Olea europaea (Ole e I) is also present in other species of the Oleaceae family.
Three major pollen allergens from Fraxinus excelsior, Ligustrum vulgare and Syringa vulgaris belonging to the Oleaceae family were purified. Monoclonal antibodies previously raised against the main allergen of Olea europaea (Ole e I) were used for their purification by affinity chromatography. The three new purified allergens were able to bind human IgE from serum of olive-allergic patients in a way analogous to Ole e I. Crossed radioimmunoelectrophoresis of the four allergens, using anti-olive extract rabbit serum, showed a unique immunoprecipitation arc with the same characteristics. The four purified proteins had similar molecular weights on SDS-PAGE and the N-terminal sequences for the first 20 amino acids were identical. Furthermore, the concentration of the allergens could be determined using a two-side solid phase assay previously developed for the allergen Ole e I. Our results indicate that the four purified proteins share, to a great extent, antigenic and allergenic epitopes leading to cross-reactivities which could cause common clinical manifestations. We propose for the newly purified allergens the nomenclature of Fra e I, Lig v I and Syr v I.
Obispo
T M
TM
Research Department, Alergia e Inmunología Abelló S.A., Madrid, Spain.
Melero
J A
JA
Carpizo
J A
JA
Carreira
J
J
Lombardero
M
M
D000485:Allergens / Q000032:analysis*; D000595:Amino Acid Sequence; D000918:Antibody Specificity; D052179:Antigens, Plant; D002846:Chromatography, Affinity; D003429:Cross Reactions; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007159:Immunologic Tests; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D010944:Plants / Q000145:classification / Q000276:immunology; D011058:Pollen / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014197:Trees / Q000145:classification / Q000276:immunology
D000485:Allergens; D052179:Antigens, Plant; C081946:Ole e I protein, Olea europaea; D010940:Plant Proteins; D007073:Immunoglobulin E