title: Biocompatibility of leukocyte removal filters during leukocyte filtration of cardiopulmonary bypass perfusate.
abstract: To evaluate the biocompatibility and the efficacy of leukocyte removal filters, we performed a prospective study by using the cardiopulmonary bypass perfusate taken from the heart-lung machine for 20 patients who underwent cardiac surgery and were randomly divided into four groups according to the filters used. A leukocyte removal filter was installed in the transfusion line while the perfusate was transfused to the patients. No increases of C3a, C5a, elastase, and thromboxane were found during leukocyte filtration by polyester filters (Optima, Sepacell R500, and Pall RC100). Activation of the complement cascade was observed during filtration by the cellulose acetate filter (Cellselect) although the efficacy of the Cellselect filter was evidently higher than that of the polyester filter. These results imply that polyester leukocyte filters are superior to cellulose acetate filters in terms of biocompatibility but have a reduced efficacy. An optimal leukocyte filter providing both high efficacy and biocompatibility has yet to be developed.
authors.affiliation: University Hospital, Groningen, The Netherlands.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Gu
authors.forename: R
authors.initials: R
authors.lastname: Obster
authors.forename: J
authors.initials: J
authors.lastname: Haan
authors.forename: R C
authors.initials: RC
authors.lastname: Gallandat Huet
authors.forename: A
authors.initials: A
authors.lastname: Eijgelaar
authors.forename: W
authors.initials: W
authors.lastname: van Oeveren
mesh_terms: D001672:Biocompatible Materials; D002315:Cardiopulmonary Bypass; D002469:Cell Separation; D002482:Cellulose / Q000031:analogs & derivatives; D003167:Complement Activation; D015926:Complement C3a / Q000032:analysis; D015936:Complement C5a / Q000032:analysis; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D007958:Leukocyte Count; D019272:Leukocyte Elastase; D007962:Leukocytes; D008213:Lymphocyte Activation; D010196:Pancreatic Elastase / Q000097:blood; D010976:Platelet Count; D011091:Polyesters; D011446:Prospective Studies; D013929:Thromboxane B2 / Q000097:blood
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C005062:acetylcellulose; D013929:Thromboxane B2; D015926:Complement C3a; D015936:Complement C5a; D002482:Cellulose; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase