title: The effects of polybrominated biphenyls and perchlorinated terphenyls on in vitro fertilization in the mouse.
abstract: Polybrominated biphenyls (PBBs) and perchlorinated terphenyls (PCTs) are industrial chemicals that are long-lasting environmental contaminants. Although in vivo effects of PBBs on reproduction are documented, no information is available on the effects of these chemicals on sperm-egg interactions or fertilization. The present study was undertaken to determine the toxic potential of PBBs and PCTs on in vitro fertilization (IVF) in the mouse. 2-Bromobiphenyl, 4-bromobiphenyl, o-terphenyl, m-terphenyl, and p-terphenyl were added to the IVF medium at various concentrations. Oocytes collected from superovulated B6D2F1 mice were maintained in a medium containing the chemicals. Capacitated sperm were then added and the dishes cultured in a humidified atmosphere at 37 degrees C in 5% CO2 + 95% air. Oocytes were assessed for fertilization 20-24 h after insemination. polybrominated biphenyls and PCTs reduced the IVF rate at the higher dosages. Furthermore, an increased incidence of abnormal two-cell embryos and degenerative oocytes was observed at the 1 and 10 micrograms/ml concentrations of PBBs and PCTs. These results indicate that PBBs and PCTs adversely effect IVF and increase the incidence of abnormal embryos and oocyte degeneration in the mouse.
authors.affiliation: Endocrine Research Center, Michigan State University, East Lansing 48824.
authors.forename: S D
authors.initials: SD
authors.lastname: Kholkute
authors.forename: J
authors.initials: J
authors.lastname: Rodriguez
authors.forename: W R
authors.initials: WR
authors.lastname: Dukelow
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D008297:Male; D051379:Mice; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D013730:Terphenyl Compounds / Q000633:toxicity*
chemical_list: D013730:Terphenyl Compounds; D011078:Polychlorinated Biphenyls