A comparison of the 8-hydroxydeoxyguanosine and micronucleus techniques for the assessment of X-ray and UV induced genotoxicity.
A comparison was made of the relative sensitivities of the cytokinesis-block micronucleus and the 8-hydroxydeoxyguanosine assays for the assessment of X-ray and UV-induced genotoxicity in mouse splenocytes in vitro. A detectable (p < 0.05) increase in micronuclei occurred at about 1/5 the X-ray exposure required to induce a significant increase in the level of 8-hydroxydeoxyguanosine. With UV radiation a significant (p < 0.05) rise in micronuclei was achieved at about 1/10th the dose needed to produce a detectable increase in 8-hydroxydeoxyguanosine levels. The data confirm the value of the cytokinesis-block micronucleus technique for the detection of genotoxicity at low-level X-irradiation, and indicate that it is more sensitive for that purpose than the 8-hydroxydeoxyguanosine assay. The data also demonstrate, for the first time, the production of 8-hydroxydeoxyguanosine by UVA/B-irradiation of intact cells, but point again to a more sensitive assessment of UV-related genotoxicity by the cytokinesis-block micronucleus method.
Kobus
K
K
Division of Human Nutrition, CSIRO, Australia, Adelaide.
Turner
J
J
Priestley
B
B
Dreosti
I
I
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002478:Cells, Cultured; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000737:chemistry / Q000528:radiation effects; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009154:Mutation / Q000528:radiation effects; D013154:Spleen / Q000528:radiation effects; D014466:Ultraviolet Rays; D014965:X-Rays
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine