[Preparation and characteristics of monoclonal antibodies to N-acetylglucosaminyl-(beta1-4)-N-acetylmuramoyl-alanyl-D-isoglutamine].
Hybridoma E6/1.2 was produced by fusion of splenocytes from mice immunized with N-acetylglucosaminyl-(beta 1-4)-N-acetylmuramyl-alanyl-D-isoglutamine (GMDP), conjugated to methylated BSA, with SP2/0 myeloma cells. GMDP-specific monoclonal antibody was IgG1 subtype and had affinity constant 2.10(9) M-1. According to competitive ELISA, the presence of the intact disaccharide fragment and the alanyl residue was critical for the GMDP-antibody interaction.
Mareeva
T Iu
TIu
Kotova
O V
OV
Makarov
E A
EA
Andronova
T M
TM
Nesmeianov
V A
VA
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000915:Antibody Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D051379:Mice; D012710:Serum Albumin, Bovine; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D012710:Serum Albumin, Bovine; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C023868:N-acetyl-beta-glucosaminyl-N-acetylmuramyl-alanylisoglutamine
Poluchenie i kharakteristika monoklonal'nykh antitel k N-atsetilgliukozaminil-(beta1-4)-N-atsetilmuramoil-alanil-D izoglutaminu.