A dipstick immunoassay using a recombinant antigen for the rapid diagnosis of bovine dictyocaulosis.
An immunoblot based dipstick test for the serodiagnosis of infections with Dictyocaulus viviparus using a recombinant antigen was evaluated. Lungworm infections in cattle could be detected with more than 99 per cent specificity and more than 99 per cent sensitivity between days 30 and 85 after experimental infection. Using ready-to-use recombinant antigen labelled filter strips, results could be obtained within 90 minutes of blood sampling. The dipstick test could be a rapid alternative method to the time-consuming faecal examination routinely used for the diagnosis of bovine dictyocaulosis.
Schnieder
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Institute of Parasitology, School of Veterinary Medicine, Hannover, Germany.
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000302:isolation & purification; D002417:Cattle; D004022:Dictyocaulus Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay / Q000379:methods; D011934:Reagent Strips; D011994:Recombinant Proteins / Q000302:isolation & purification
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011934:Reagent Strips; D011994:Recombinant Proteins