[In vitro analysis for cellular toxicity of polychlorinated biphenyls (PCBs) and 2,3,4,7,8-pentacholorodibenzofuran (PCDF) on PLC/PRF/5 cell proliferation (II)--The effects of ursodeoxycholic acid and chenodeoxycholic acid on cell toxicity].
We investigated the cell toxicity of polychlorinated biphenyls (PCBs) and 2,3,4,7,8-pentachlorodibenzofuran (PCDF) as two kinds of indicator of the quantity of secreting protein, which is an HBV surface antigen (HBsAg) in PLC/PRF/5 cells, and the DNA of those cells was counted the radioactivity for dot hybridization method, respectively. Furthermore, the reductive action of ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) on the PCBs and PCDF toxicity was investigated. HBsAg titer increased to 10 to 15% with the addition of CDCA at the concentration of 0.02 x 2(-6)%. However, the slope of the curve of DNA synthesis of HeLa cells at the presence of PCDF was gradually increased at the concentration of 0.02 x 2(-4)% of UDCA and CDCA, and it became to overlap with a control group while PCBs did not. These results mean that PCDF cell toxicity was suppressed a little by UDCA and CDCA, but the case of PCBs did not.
Matsuoka
K
K
Division of Pharmacology, Ureshino National Hospital, Saga.
Nonaka
S
S
Ohgami
T
T
Yoshida
H
H
D001572:Benzofurans / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002635:Chenodeoxycholic Acid / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D006367:HeLa Cells; D006451:Hemoglobin, Sickle / Q000032:analysis; D006801:Humans; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
D001572:Benzofurans; D006451:Hemoglobin, Sickle; D002635:Chenodeoxycholic Acid; D014580:Ursodeoxycholic Acid; D004247:DNA; D011078:Polychlorinated Biphenyls; C038890:2,3,4,7,8-pentachlorodibenzofuran