Determination of diltiazem hydrochloride in human serum by high-performance liquid chromatography.
A simple and sensitive reversed-phase high-performance liquid chromatographic method for the determination of diltiazem in human serum has been developed. The method involves a one-step deproteinization of serum for sample clean-up using acetonitrile. A LiChrosorb RP-8 column (30 cm x 4.1 mm I.D.) was eluted isocratically with acetonitrile-0.01 M dibasic sodium phosphate (40:60, v/v) containing 0.01% triethanolamine. Diltiazem was monitored at 237 nm and 0.1 a.u.f.s. The completion time for assay was less than 15 min, and the lower limit of quantitation was 10 ng/ml for a 100-microliters injection volume. Using this method, the pharmacokinetic parameters were calculated from a serum concentration versus time profile of diltiazem.
Chaudhary
R S
RS
Lupin Laboratories Limited, MIDC, Chikalthana, Aurangabad, India.
Gangwal
S S
SS
Avachat
M K
MK
Shah
Y N
YN
Jindal
K C
KC
D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004110:Diltiazem / Q000097:blood* / Q000493:pharmacokinetics; D004983:Ethanolamines; D006801:Humans; D007700:Kinetics; D012972:Sodium Hydroxide
D000097:Acetonitriles; D004983:Ethanolamines; D012972:Sodium Hydroxide; C009546:triethanolamine; D004110:Diltiazem; C032159:acetonitrile