High-performance liquid chromatographic determination of the anthelmintic mebendazole in eel muscle tissue.
A simple high-performance liquid chromatographic method for the determination of mebendazole in eel muscle tissue was developed. It is applicable for concentrations of 10 micrograms/kg and higher. Muscle tissue was extracted with ethyl acetate, and the extract, after addition of hexane, was concentrated and cleaned up on a silica gel solid-phase extraction column. Mebendazole was eluted from the column with 3% acetic acid in methanol. The eluate, after reconstitution in the chromatographic mobile phase, was analysed on a LiChrosorb RP-8 column with acetonitrile-0.05 M ammonium phosphate buffer (pH 6.2) (3:7, v/v) as the mobile phase. Detection was performed at 311 nm. The average mebendazole recovery over the concentration range 10-5000 micrograms/kg eel muscle tissue was 72 +/- 6.4%. The calibration curve for spiked samples was linear throughout the range 10-10,000 micrograms/kg (r = 0.999).
Steenbaar
J G
JG
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, University of Utrecht, Netherlands.
Hajee
C A
CA
Haagsma
N
N
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004524:Eels / Q000378:metabolism*; D007202:Indicators and Reagents; D008463:Mebendazole / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D008463:Mebendazole