Effect of TGF-alpha on growth of normal human breast epithelial cells in serum-free primary culture using 3-dimensional collagen gels.
The mitogenic effect of TGF-alpha, acidic-FGF, basic-FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum-free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF-alpha elicited a strong mitogenic response in a dose dependent manner. Addition of cortisol to TGF-alpha stimulated growth over and above that achieved with TGF-alpha alone. A consistent observation has been the effect of a combination of TGF-alpha and cortisol on growth stimulation of normal human breast epithelial cells resulting in 3-12 fold growth after 11-13 days in culture. Acidic-FGF, basic-FGF and lithium were not growth promoting.
Sakthivel
R
R
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Hamdan
M
M
Yang
J
J
Guzman
R C
RC
Nandi
S
S
D001940:Breast / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D003094:Collagen; D016895:Culture Media, Serum-Free; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*
D016895:Culture Media, Serum-Free; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D003094:Collagen; D006854:Hydrocortisone