title: Plasminogen activation activity of urokinase determined via a cascade enzymatic reaction system.
abstract: A method for determining the plasminogen activation rate by urokinase via a cascade enzymatic reaction system is presented. A procedure of parameter estimation has been proposed for the determination of the activity of urokinase and the kinetic constants. Urokinase from urine has been successfully assayed through use of a plasminogen concentration lower than its saturating level. The methodology presented in this work may be adopted for the analysis of other cascade enzymatic reaction systems.
authors.affiliation: Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taiwan.
authors.forename: W C
authors.initials: WC
authors.lastname: Lee
authors.forename: C Y
authors.initials: CY
authors.lastname: Chuang
authors.forename: R M
authors.initials: RM
authors.lastname: Chang
mesh_terms: D006801:Humans; D007700:Kinetics; D008433:Mathematics; D010958:Plasminogen / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism* / Q000652:urine
chemical_list: D010958:Plasminogen; D014568:Urokinase-Type Plasminogen Activator