[The effect of methotrexate on phenobarbital induction of monooxygenases and UDP-glucuronyltransferases in rat liver].
Methotrexate (N10-methyl-4-amino-4-deoxyfolic acid), administered to rats subcutaneously at a dose of 0.25 mg/kg within 14 days, was found to decrease content of microsomal protein in liver tissue and content of cytochromes P-450 and b5 as well as the drug decreased activity of amidopyrine- and ethylmorphine-N-demethylases, aniline-p-hydroxylase, UDP-glucuronosyltransferase and urinary excretion of glucuronides by 21%, 60%, 39%, 33%, 53%, 21%, 41% and 28%, respectively. Phenobarbital, administered subcutaneously at a dose of 60 mg/kg within 14 days, elevated the parameters by 28%, 187%, 63%, 163%, 162%, 100%, 95% and 60%, respectively. The effect of phenobarbital in combination with methotrexate was decreased by 77%, 49%, 55%, 52%, 43%, 42% and 22%, respectively.
Lukienko
P I
PI
Legon'kova
L F
LF
Bushma
M I
MI
Shoka
A Iu
AIu
Zverinskiĭ
I V
IV
Zavodnik
L B
LB
D000818:Animals; D004790:Enzyme Induction; D005494:Folic Acid Deficiency / Q000201:enzymology; D005965:Glucuronates / Q000652:urine; D014453:Glucuronosyltransferase / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008727:Methotrexate / Q000494:pharmacology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D010634:Phenobarbital / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats
D005965:Glucuronates; D006899:Mixed Function Oxygenases; D014453:Glucuronosyltransferase; D008727:Methotrexate; D010634:Phenobarbital
Vliianie metotreksata na fenobarbitalovuiu induktsiiu monoksigenaz i UDF-gliukuronoziltransferazy pecheni krys.