title: The amino acid sequences of human and pig L-arginine:glycine amidinotransferase.
abstract: We have isolated and sequenced the L-arginine:glycine amidinotransferase of pig kidney mitochondria. Due to endogenous proteolysis, the purified molecules showed some heterogeneity at the N terminus. The longest form recovered had 386 amino acids. Part of the pig amidinotransferase sequence information was used to isolate cDNA clones coding for the human enzyme. The deduced amino acid of the human amidinotransferase was 37 amino acids longer due to the presence of a single sequence. The mature proteins were 94% identical to each other and 36% identical to the sequences of bacterial L-arginine:inosamine phosphate amidinotransferases.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Humm
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: K
authors.initials: K
authors.lastname: Mann
mesh_terms: D000579:Amidinotransferases / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003545:Cysteine; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007672:Kidney Cortex / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism; D021382:Protein Sorting Signals / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D017421:Sequence Analysis; D017385:Sequence Homology; D013552:Swine
chemical_list: D018076:DNA, Complementary; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D000579:Amidinotransferases; C020681:glycine amidinotransferase; D003545:Cysteine