Quantitation of propofol in plasma by capillary gas chromatography.
A rapid, accurate and sensitive gas chromatographic method is described for measuring plasma concentrations of propofol. The technique required only 200 microliters of plasma and a single extraction process, using chloroform containing pentadecane (500 ng/ml) as an internal standard. Quantitation was achieved on an SGE BP-1 fused-silica capillary column (25 m x 0.33 mm I.D., 0.5 micron film thickness) with flame ionization detector. The peak response was linear over a wide concentration range (10-10,000 ng/ml) and the limit of quantitation was 10 ng/ml. The absolute recoveries were over 96% (n = 3). The method is applicable for both research and routine plasma level monitoring.
Yu
H Y
HY
School of Pharmacy, National Taiwan University, Taipei.
Liau
J K
JK
D002849:Chromatography, Gas; D006801:Humans; D015742:Propofol / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards
D015742:Propofol