A heat stable serum factor upregulates 5-lipoxygenase activity in HL-60 cells, modulation by TNF alpha or GM-CSF.
5-Lipoxygenase (5-LO) activity in differentiating HL-60 cells was upregulated by a heat stable protein in human serum. Hematopoietic cytokines were evaluated for this effect on 5-lipoxygenase activity, none of the compounds tested could replace serum. However, TNF alpha or GM-CSF were able to augment the effect of heat treated serum, giving 5-LO activities that (per cell) were higher than for granulocytes isolated from peripheral blood. The serum factor primarily upregulated 5-LO activity. The amounts of 5-LO protein, or mRNA for 5-LO or 5-lipoxygenase activating protein (FLAP), were less affected.
Steinhilber
D
D
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Rådmark
O
O
Samuelsson
B
B
D058927:5-Lipoxygenase-Activating Proteins; D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis* / Q000302:isolation & purification; D001483:Base Sequence; D001790:Blood Physiological Phenomena; D015153:Blotting, Western; D002352:Carrier Proteins / Q000096:biosynthesis*; D003470:Culture Media; D016207:Cytokines / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D008565:Membrane Proteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D058927:5-Lipoxygenase-Activating Proteins; C548592:ALOX5AP protein, human; D002352:Carrier Proteins; D003470:Culture Media; D016207:Cytokines; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D001094:Arachidonate 5-Lipoxygenase