title: Portal transport of long acyl chain lipids: effect of phosphatidylcholine and low infusion rates.
abstract: The transport of absorbed long acyl chain lipids in the portal vein of rats has been shown to be 39% when the duodenal input rate is 135 mumol/h glyceryl trioleate (TO) [C. M. Mansbach II, R. F. Dowell, and D. Pritchett, Am. J. Physiol. 255 (Gastrointest. Liver Physiol. 18): G530-G539, 1991]. These calculations were based on a new experimental model in which portal flux is calculated from the knowledge of portal flow and the concentration of the lipids in excess in the portal vein vs. the carotid artery. To test this model, rats were infused for 6 h with a low rate of [3H]TO (27 mumol/h) with or without phosphatidylcholine (9 mumol/h) or with [3H]TO (135 mumol/h) plus phosphatidylcholine (9 mumol/h) or with [3H]TO (135 mumol/h) plus phosphatidylcholine (9 mumol/h). In all three cases, portal flux was expected to be less. Portal transport was 16.5% of the input rate in the low-dose group, 1.4% in the high-dose group given phosphatidylcholine, and 0.5% in the low-dose plus phosphatidylcholine group. There was no net transport of fatty acid in the portal vein in any of the three cases. These data show that portal lipid transport is dependent on the lipid load and that it is greatly reduced at high loads by including phosphatidylcholine in the lipid infusion.
authors.affiliation: Department of Medicine, University of Tennessee, Memphis 38163.
authors.forename: C M
authors.initials: CM
authors.lastname: Mansbach
authors.forename: R F
authors.initials: RF
authors.lastname: Dowell
mesh_terms: D000818:Animals; D001692:Biological Transport; D004338:Drug Combinations; D008055:Lipids / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry*; D008297:Male; D010713:Phosphatidylcholines / Q000008:administration & dosage / Q000494:pharmacology*; D011169:Portal Vein / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014304:Triolein / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D004338:Drug Combinations; D008055:Lipids; D010713:Phosphatidylcholines; D014304:Triolein