title: Genetic events in sporadic colorectal adenomas: K-ras and p53 heterozygous mutations are not sufficient for malignant progression.
abstract: Twenty-four sporadic colorectal adenomas were analysed for the presence of allelic loss on the short arm of chromosome 17 as well as mutations in the K-ras and p53 genes. Chromosome 17p13 allelic loss was not present in 14 out of 14 informative cases. K-ras mutations were observed in 15 out of 24 cases. A p53 gene mutation (GGC-->GAC at codon 245) was detected in two biopsies taken at a four year interval from a recurrent rectal villous adenoma. Both biopsies also contained the same K-ras gene mutation (GGT-->GTT at codon 12). The data from the recurrent rectal adenoma provide in vivo evidence that K-ras and p53 heterozygous mutations confer a proliferative advantage but together are not sufficient for malignant transformation.
authors.affiliation: IST, National Cancer Institute, Genova, Italy.
authors.forename: L
authors.initials: L
authors.lastname: De Benedetti
authors.forename: L
authors.initials: L
authors.lastname: Varesco
authors.forename: N S
authors.initials: NS
authors.lastname: Pellegata
authors.forename: L
authors.initials: L
authors.lastname: Losi
authors.forename: V
authors.initials: V
authors.lastname: Gismondi
authors.forename: L
authors.initials: L
authors.lastname: Casarino
authors.forename: S
authors.initials: S
authors.lastname: Sciallero
authors.forename: L
authors.initials: L
authors.lastname: Bonelli
authors.forename: R
authors.initials: R
authors.lastname: Biticchi
authors.forename: A
authors.initials: A
authors.lastname: Bafico
mesh_terms: D000236:Adenoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17; D003062:Codon / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D003062:Codon