Effects of androgens on isolated rat uterus.
The 5 alpha- and 5 beta-dihydrotestosterone (DHT) androgens relax KCl-induced tonic contraction in rat uterus, in a dose-dependent way. The 5 alpha and 5 beta-DHT relaxing effect is counteracted by CaCl2 (0.1-10 mM). The 5 alpha-DHT, but not the 5 beta-DHT, effect was reduced by cycloheximide (10 micrograms/ml) and by actinomycin D (5 micrograms/ml). Flutamide at 10(-6) M shifted the effect of 5 alpha-DHT to the right. However, other doses of flutamide or cyproterone acetate did not modify the effect of both androgens. We suggest a non-genomic effect of 5 alpha-DHT and 5 beta-DHT in rat uterus contraction but that intracellular and genomic actions play a part in the relaxing effect of 5 alpha-DHT.
Sánchez Aparicio
J A
JA
Departamento de Medicina, Facultad de Medicina, Oviedo, Spain.
Gutierrez
M
M
Hidalgo
A
A
Cantabrana
B
B
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D017373:Cyproterone Acetate / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004347:Drug Interactions; D005260:Female; D005485:Flutamide / Q000494:pharmacology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000187:drug effects* / Q000502:physiology
D013196:Dihydrotestosterone; D003609:Dactinomycin; D017373:Cyproterone Acetate; D005485:Flutamide; D003513:Cycloheximide