Inhibition of cell proliferation by Rana catesbeiana and Rana japonica lectins belonging to the ribonuclease superfamily.
Two frog egg lectins [Rana catesbeiana lectin (SBL-C) and Rana japonica lectin] preferentially agglutinate a large variety of human and animal tumor cells but not blood cells, lymphocytes, or fibroblasts. These lectins belong to the superfamily of pyrimidine base-specific RNases. The two lectins bound to a heparin-Sepharose column and were eluted from the column by an increase of NaCl molarity. Both their tumor cell-agglutinating activity and RNase activity were inhibited by heparin, and also by polyamines, such as spermine. Both lectins inhibited P388 leukemia cell proliferation. The inhibitory activity of SBL-C was blocked by addition of heparin. SBL-C inhibited protein synthesis by P388 cells, but RNase A did not. No lectin-induced antiproliferative effect was observed after sialidase treatment of cells. The antiproliferative activity of SBL-C was also inhibited by ammonium chloride treatment. These results suggest that internalization of the lectins by lectin receptor (sialoglycoconjugate)-mediated endocytosis is followed by cell death due to inhibition of protein synthesis. Administration of SBL-C i.p. delayed time to death in mice receiving i.p. transplants of Sarcoma 180 and Mep II cells.
Nitta
K
K
Cancer Research Institute, Tohoku College of Pharmaceutical Sciences, Sendai, Japan.
Ozaki
K
K
Ishikawa
M
M
Furusawa
S
S
Hosono
M
M
Kawauchi
H
H
Sasaki
K
K
Takayanagi
Y
Y
Tsuiki
S
S
Hakomori
S
S
D000371:Agglutination; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002846:Chromatography, Affinity; D006001:Glycoconjugates / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000401:mortality; D011073:Polyamines / Q000494:pharmacology; D011892:Rana catesbeiana; D011898:Ranidae; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology*
D000970:Antineoplastic Agents; D006001:Glycoconjugates; D006025:Glycosaminoglycans; D037102:Lectins; D011073:Polyamines; D012260:Ribonucleases