Determination of cortisol and associated glucocorticoids in serum and urine by an automated liquid chromatographic assay.
We describe a method for the determination of urinary free cortisol and glucocorticoids in plasma, used in the diagnosis of adrenal disorders, based on automated reverse-phase high-performance liquid chromatography (HPLC). The within-day and day-to-day CVs were less than 5.5 and 8.0%, respectively. The calibration curves for cortisol and 11-deoxycortisol were linear up to 2000 nmol/L. Cortisol concentrations as low as 3.5 nmol/L in 1 mL of plasma or urine can be measured. Correlation of HPLC results for 40 plasma specimens with those by radioimmunoassay showed r = 0.965. This method is sensitive and free from the interference habitually encountered in immunoassays, and can thus be proposed for research and as a potential reference method.
Samaan
G J
GJ
Service de Biochimie-Hormonologie, Hôpital R. Debre, Paris, France.
Porquet
D
D
Demelier
J F
JF
Biou
D
D
D000293:Adolescent; D000307:Adrenal Gland Diseases / Q000175:diagnosis; D001667:Binding, Competitive; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003350:Cortodoxone / Q000097:blood* / Q000652:urine; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood* / Q000652:urine*; D007223:Infant; D008297:Male; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity
D003350:Cortodoxone; D006854:Hydrocortisone