title: Analysis of the 11p13 Wilms' tumor suppressor gene (WT1) in ovarian tumors.
abstract: We have examined the status of the Wilms' tumor suppressor gene (WT1), residing at chromosome 11 band p13, in a total of 40 cancers of the female reproductive tract. Northern blot analysis revealed that the WT1 gene is expressed in a large percentage of ovarian tumors (75%) analyzed. Single-strand conformation polymorphism analysis was performed on all the tumors in this study in an attempt to detect mutations within the WT1 gene. Only silent mutations were detected within intron 7 of WT1 using this method. Loss of heterozygosity studies were performed at the WT1 locus in several ovarian tumors and revealed that in the informative cases, heterozygosity was retained.
authors.affiliation: Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Bruening
authors.forename: P
authors.initials: P
authors.lastname: Gros
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: J
authors.initials: J
authors.lastname: Stanimir
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: D
authors.initials: D
authors.lastname: Housman
authors.forename: J
authors.initials: J
authors.lastname: Pelletier
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015870:Gene Expression; D016162:Genes, Wilms Tumor / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012334:RNA, Neoplasm / Q000032:analysis
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm