The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells.
The expression of regulatory genes within the context of a differentiation program can have profound long-term consequences in tissues with permanent renewing populations. The breast tumor cell line MCF-7 retains in culture some of the characteristics of a unidirectional differentiation pathway. We show that the cytosolic activity of the regulatory enzyme protein kinase C (PKC) precedes and continues the sequence of maturation in pre-differentiated subpopulations derived from a stem cell fraction. However, the activity declines in the most differentiated, postmitotic fraction. These results indicate that PKC may be considered among the regulatory genes in MCF-7 cells that specify maturation of the stem cell progeny.
Gomez
M L
ML
Instituto de Ingeniera Genetica y Biologia Molecular (INGEBI, CONICET), Buenos Aires, Argentina.
Resnicoff
M
M
Medrano
E E
EE
Tellez-Inon
M T
MT
D001943:Breast Neoplasms / Q000378:metabolism*; D002454:Cell Differentiation; D003600:Cytosol / Q000378:metabolism; D005260:Female; D005809:Genes, Regulator; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D006133:Growth Substances; D011493:Protein Kinase C