Changes in proteoglycan core protein (PCP) mRNA levels during HL-60 cell differentiation.
The steady-state level of the PCP mRNA was investigated during HL-60 cell differentiation. When cells were induced to differentiate into granulocytes with 1.3% dimethylsulfoxide or 1 microM cis or trans retinoic acid, no significant changes in PCP mRNA levels were observed. In contrast, cells cultured in the presence of the phorbol ester TPA, which promotes the cells to be differentiated into monocytes/macrophages, were associated with a marked decrease in the steady-state concentration of PCP mRNA. When cells were simultaneously treated with a combination of TPA and staurosporine, a protein kinase C (PKC) inhibitor, the TPA-elicited decrease in PCP mRNA was partially prevented. These data suggest that PCP mRNA expression is not directly linked to HL-60 cell differentiation but appears to involve the participation of PKC.
Whyzmuzis
C A
CA
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Wu
J M
JM
Danishefsky
I
I
D053674:Aggrecans; D000470:Alkaloids / Q000494:pharmacology; D015152:Blotting, Northern; D002454:Cell Differentiation; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D016326:Extracellular Matrix Proteins; D015870:Gene Expression / Q000187:drug effects; D016259:Genes, myc; D006023:Glycoproteins / Q000096:biosynthesis*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D037181:Lectins, C-Type; D015473:Leukemia, Promyelocytic, Acute; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011509:Proteoglycans; D012333:RNA, Messenger / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000096:biosynthesis; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D053674:Aggrecans; D000470:Alkaloids; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D009363:Neoplasm Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide