Prolonged normothermic perfusion of the isolated bovine eye: initial results.
This paper describes initial results of experiments with a prolonged normothermic arterial perfusion of isolated bovine eyes. The eyes were perfused using oxygenated serum-free MEM. With respect to utilization of oxygen and use of glucose, viability could be proven for 5 days. After addition of some essential serum substitutes, viability could be extended to 9 days, moreover, doubled glucose utilization and decreasing LDH activity could also be measured. To prolong and improve viability, much attention should be paid to oxygenation and composition of the perfusate.
de Coo
F A
FA
Department of Internal Medicine, University Hospital Utrecht, The Netherlands.
Zonnenberg
B A
BA
Trap
N H
NH
D000818:Animals; D002417:Cattle; D002470:Cell Survival; D016895:Culture Media, Serum-Free; D005123:Eye / Q000378:metabolism / Q000473:pathology; D005947:Glucose / Q000378:metabolism; D009799:Ocular Physiological Phenomena; D010101:Oxygen Consumption / Q000502:physiology; D010477:Perfusion / Q000379:methods*; D013696:Temperature
D016895:Culture Media, Serum-Free; D005947:Glucose