title: Inhalant atopic sensitivity to grasshoppers in research laboratories.
abstract: BACKGROUND
  Atopic sensitivity to insects, in both occupational and nonoccupational settings, is common.
  
  
  METHODS
  A 26-year-old man with atopic asthma experienced worsened asthma and urticaria on exposure to grasshoppers in a research laboratory; he along with 16 other persons who work with grasshoppers from two laboratories and 26 control subjects were studied. The patient underwent a controlled allergen inhalation test with aqueous grasshopper dropping antigen. All subjects were assessed by means of a questionnaire. All but one (who refused because of severe skin reactions after contact with grasshoppers) had skin prick tests with three extracts of grasshopper and with grass pollen, cat dander, and Dermatophagoides farinae.
  
  
  RESULTS
  The allergen challenge was positive with an isolated early asthmatic response (23% fall forced expiratory volume in 1 second [FEV1]) at 1:4096 (approximately 25 micrograms/ml), and a borderline fall in provocative concentration of methacholine causing a 20% fall in FEV1. Seven of 16 (43.8%) workers had positive grasshopper skin test results compared with one of 26 (3.8%) control subjects (p = 0.0052). Sensitization occurred even in otherwise nonatopic workers (5 of 12). Symptoms of asthma on exposure (n = 4) correlated better with positive skin test results than did cutaneous symptoms (n = 8).
  
  
  CONCLUSIONS
  Atopic sensitization to grasshoppers in research laboratories is a significant occupational health problem.
authors.affiliation: Department of Medicine, Royal University Hospital, University of Saskatchewan, Saskatoon, Canada.
authors.forename: G R
authors.initials: GR
authors.lastname: Soparkar
authors.forename: P C
authors.initials: PC
authors.lastname: Patel
authors.forename: D W
authors.initials: DW
authors.lastname: Cockcroft
mesh_terms: D000328:Adult; D000818:Animals; D000941:Antigens; D001249:Asthma / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology; D001985:Bronchial Provocation Tests / Q000379:methods / Q000706:statistics & numerical data; D016009:Chi-Square Distribution; D006110:Grasshoppers / Q000276:immunology*; D006801:Humans; D008297:Male; D017056:Medical Laboratory Personnel / Q000706:statistics & numerical data; D009784:Occupational Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology*; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology*; D012220:Rhinitis / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology; D012882:Skin Tests / Q000379:methods / Q000706:statistics & numerical data; D014581:Urticaria / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology
chemical_list: D000941:Antigens