Transmission of Chlamydia trachomatis among sex partners assessed by polymerase chain reaction.
The prevalence of Chlamydia infection in 95 sex partners was determined by both polymerase chain reaction (PCR) and cell culture. Thirty-three (18%) of 186 specimens were positive by culture and 61 (33%) were positive by PCR-EIA. PCR was positive in 75% (21/28) of male partners of PCR-positive women compared with culture, which was positive in only 45% (9/19) of male partners of culture-positive women (P = .053). For female partners of infected men, the difference was less marked. PCR was positive in 58% (21/36) of female partners of infected men versus culture, which was positive in 56% (15/36) of female partners of culture-positive men. The correlation of PCR between partners and sequence analysis of Chlamydia DNA showing the same sequence from sex partners of 7 couples support the accuracy of the assay. These data suggest that PCR is more sensitive than culture for detection of Chlamydia trachomatis, particularly for male partners of infected women.
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Division of Infectious Diseases (Department of Pediatrics), Johns Hopkins University School of Medicine, Baltimore, Maryland.
Bobo
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Hook
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Quinn
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D000328:Adult; D001483:Base Sequence; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000635:transmission*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012747:Sexual Partners
D004269:DNA, Bacterial