Lipid hydroperoxide-resistance gene in Saccharomyces cerevisiae: utilization as a selectable marker gene for yeast transformation.
Two kinds of DNA fragments concerned with resistance against lipid hydroperoxide were cloned from the yeast Saccharomyces cerevisiae. When the recombinant plasmid carrying the DNA fragment was introduced into the cells of S. cerevisiae which do not have an appropriate auxotrophic marker, the transformants could be selected by their resistance to t-butyl hydroperoxide. By Southern hybridization analysis, such transformants were found to contain the recombinant plasmid, suggesting that the DNA fragment could be used as a selection marker for the transformation of S. cerevisiae.
Inoue
Y
Y
Research Institute for Food Science, Kyoto University, Japan.
Kobayashi
S
S
Yoshikawa
K
K
Tran
L T
LT
Kimura
A
A
D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D015698:Genomic Library; D008054:Lipid Peroxides / Q000494:pharmacology*; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic
D008054:Lipid Peroxides