Removal of stainable cytoplasmic substances from cytogenetic slide preparations.
A method to remove stainable cytoplasmic substances from cytogenetic preparation using RNase A treatment is reported. The preparations processed with this method are especially useful for the automated analysis of micronuclei of cultured cells with cytochalasin B and of chromosome aberrations induced by radiation.
Hayata
I
I
Division of Radiation Hazards, National Institute of Radiological Sciences, Chiba-shi, Japan.
D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure; D003571:Cytochalasin B; D003593:Cytoplasm / Q000648:ultrastructure*; D006801:Humans; D007621:Karyotyping; D008677:Metaphase; D048629:Micronuclei, Chromosome-Defective / Q000648:ultrastructure; D012259:Ribonuclease, Pancreatic
D003571:Cytochalasin B; D012259:Ribonuclease, Pancreatic