Anhidrotic ectodermal dysplasia gene region cloned in yeast artificial chromosomes.
Anhidrotic ectodermal dysplasia (EDA), an X-chromosomal recessive disorder, is expressed in a few females with chromosomal translocations involving bands Xq12-q13. Using available DNA markers from the region and somatic cell hybrids we mapped the X-chromosomal breakpoints in two such translocations. The breakpoints were further mapped within a yeast artificial chromosome contig constructed by chromosome walking techniques. Genomic DNA markers that map between the two translocation breakpoints were recovered representing putative portions of the EDA gene.
Kere
J
J
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Grzeschik
K H
KH
Limon
J
J
Gremaud
M
M
Schlessinger
D
D
de la Chapelle
A
A
D001483:Base Sequence; D015825:Chromosomes, Fungal; D002878:Chromosomes, Human, Pair 1 / Q000648:ultrastructure; D002899:Chromosomes, Human, Pair 9 / Q000648:ultrastructure; D004476:Ectodermal Dysplasia / Q000235:genetics*; D005260:Female; D015723:Gene Library; D005796:Genes; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014178:Translocation, Genetic; D014960:X Chromosome / Q000648:ultrastructure
D005819:Genetic Markers