GH3 cells, an anterior pituitary cell line, express CCK-B receptors.
We found that GH3 cells, a rat anterior pituitary tumor cell line, expressed a single class of high-affinity binding sites for radiolabeled cholecystokinin octapeptide (CCK-8) with a Kd of 48 pM. The binding sites had high affinity for CCK-8, CCK-4, gastrin I, and L-365,260 (CCK-B antagonist), and had low affinity for devazepide (CCK-A antagonist), indicating that the binding sites are CCK-B receptors. GTP and its stable analogues inhibited radiolabeled CCK-8 binding to GH3 cell membranes, suggesting a coupling of CCK-B receptors to a G-protein.
Kuwahara
T
T
Department of Pharmaceutical Screening, Nippon Roche Research Center, Kamakura, Japan.
Takamiya
M
M
Nagase
H
H
Kudoh
T
T
Nakano
A
A
Yoshizaki
H
H
Arisawa
M
M
D000818:Animals; D010903:Pituitary Gland, Anterior / Q000737:chemistry* / Q000166:cytology; D011869:Radioligand Assay; D051381:Rats; D011949:Receptors, Cholecystokinin / Q000032:analysis*; D014407:Tumor Cells, Cultured
D011949:Receptors, Cholecystokinin