Evaluation of an immunoperoxidase technique using an only biotin-labeled antibody for the demonstration of Actinobacillus pleuropneumoniae in tissue sections.
In this report an immunoperoxidase technique (IPB) using an only biotin-labeled antibody is compared to culture isolation method (CIM) for the demonstration of Actinobacillus pleuropneumoniae serotype 1 in tissues of mice inoculated intravenously. The organism was isolated in 86.7% of mice and detected by IPB in 100% of cases. A. pleuropneumoniae antigen was mainly demonstrated in the liver, spleen and lungs, but also in the kidney and brain, being especially located in the cytoplasm of macrophages. Significant differences were observed between the results obtained by both tests (P < 0.001). Cross-reactions occurred by IPB when a rabbit anti-A. pleuropneumoniae serotype 11 serum was tested but never when an anti-serotype 9 serum was used. The immunoperoxidase test here described yielded much better results than CIM and it could be useful in routine diagnosis of A. pleuropneumoniae infections.
Gutierrez
C B
CB
Department of Animal Pathology (Animal Health), Faculty of Veterinary Medicine, León, Spain.
Rodriguez-Barbosa
J I
JI
Suarez
J
J
Tascon
R I
RI
Rodriguez-Ferri
E F
EF
D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000302:isolation & purification*; D000818:Animals; D000907:Antibodies, Bacterial; D001710:Biotin; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D008297:Male; D051379:Mice; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D001710:Biotin