[Biological safety investigations of the production of human insulin by genetically-engineered E. coli K-12 cells. 3. Plasmid transfer by natural transformation and transduction].
The transfer of the expression plasmid of human insulin pSW3 by natural transformation and by transduction with phage P1 was investigated in laboratory media and in surface water samples. Whereas Pseudomonas stutzeri could be transformed by shuttle vectors which could replicate in this microorganism, no transformants were found with the insulin expression vector. E. coli K-12 could be transduced by phage P1vir only with chromosomal markers, not with plasmid DNA. As the production strain W3110iqM15 (pSW3) did not grow in river water, no phage lysates could be isolated under these conditions. The production strain had a reduced fitness compared to the plasmid free parental strain, induction of insulin expression resulted in deletions of plasmid DNA.
Mieschendahl
M
M
Battelle-Institut e.V., Frankfurt/M.
Schaffrath
S
S
Lenkeit
C
C
Danneberg
G
G
D000668:Ampicillin Resistance; D017101:Bacteriophage P1; D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans; D007328:Insulin / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics; D014161:Transduction, Genetic; D014169:Transformation, Bacterial; D014871:Water Microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D007328:Insulin
Biologische Sicherheitsforschung zur Produktion von Humaninsulin mit gentechnisch veränderten E. coli K-12 Zellen. 3. Mitteilung: Plasmid-Transfer durch natürliche Transformation and Transduktion.