Microsatellite polymorphisms for chromosome 5 bands q11.2-q13.3.
The genes for spinal muscular atrophy (SMA) and a possible subtype of schizophrenia (SCZD1) have been mapped to chromosome 5q11.2-q13.3. DNA markers have been mapped to 5q11.2-q13.3 using a hybrid cell line deleted for this region [Gilliam et al., Genomics 1989;5:940-944]. Genomic lambda clones for these markers facilitated the identification of highly polymorphic microsatellites. A total of ten microsatellites were identified and sequenced. Of these, seven were found to be polymorphic. Four had polymorphism information content values > 0.7. New polymorphic microsatellites were sequenced for D5S76, D5S125, D5S39, D5S127 and HEX-B. Two-point and multipoint analysis in non-CEPH pedigrees confirmed that the microsatellites were in tight linkage with each other. These new microsatellites will increase the efficiency of linkage analysis for these disorders.
Sherrington
R
R
Academic Department of Psychiatry, University College and Middlesex, School of Medicine, London, UK.
Mankoo
B
B
Dixon
M
M
Curtis
D
D
Kalsi
G
G
Melmer
G
G
Gurling
H
H
D000483:Alleles; D001483:Base Sequence; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000737:chemistry*; D005787:Gene Frequency; D015723:Gene Library; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009134:Muscular Atrophy, Spinal / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012559:Schizophrenia / Q000235:genetics*
D004276:DNA, Satellite; D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides