title: Determination of chloroacetaldehyde, a metabolite of oxazaphosphorine cytostatic drugs, in plasma.
abstract: A derivatization high-performance liquid chromatographic method with ultraviolet detection to monitor the plasma concentration of chloroacetaldehyde, a neurotoxic metabolite of oxazaphosphorine drugs, is presented. To prevent the rapid degradation of chloroacetaldehyde, the plasma samples are stabilized with formaldehyde. The method is linear in the concentration range 1-250 nmol/ml. Blood samples from a patient who was treated with a ten-day continuous infusion of ifosfamide were assayed. The chloroacetaldehyde concentrations did not exceed 10 nmol/ml.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, Netherlands.
authors.forename: G P
authors.initials: GP
authors.lastname: Kaijser
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
authors.forename: E L
authors.initials: EL
authors.lastname: Jeunink
authors.forename: A
authors.initials: A
authors.lastname: Bult
authors.forename: H J
authors.initials: HJ
authors.lastname: Keizer
authors.forename: J
authors.initials: J
authors.lastname: de Kraker
authors.forename: W J
authors.initials: WJ
authors.lastname: Underberg
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000097:blood; D000368:Aged; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005557:Formaldehyde; D006801:Humans; D007069:Ifosfamide / Q000493:pharmacokinetics*
chemical_list: D005557:Formaldehyde; C004656:chloroacetaldehyde; D000079:Acetaldehyde; D007069:Ifosfamide