Stimulation of B lymphocytes by lipopolysaccharides from anaerobic bacteria.
Lipopolysaccharides (LPSs) from anaerobic gram-negative bacteria, including those of low endotoxic activity that are isolated from Bacteroides, Prevotella, and Porphyromonas are potent inducers of DNA replication and polyclonal immunoglobulin production in murine B lymphocytes. The activation is dose-dependent and T cell-independent. Replication of DNA and production of immunoglobulins were also stimulated by lipid A and by the LPS heteropolysaccharide that were isolated by mild acid hydrolysis of the LPSs of Bacteroides fragilis and Fusobacterium nucleatum. Combinations of LPS, lipid A, and acid-degraded polysaccharide amplified the blastogenic response. Antibodies that react with the polysaccharide part of LPSs isolated from members of the Bacteroidaceae are present in healthy human serum.
Hofstad
T
T
Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.
Skaug
N
N
Sveen
K
K
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D001421:Bacteria, Anaerobic; D002240:Carbohydrate Sequence; D006801:Humans; D066298:In Vitro Techniques; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure
D008050:Lipid A; D008070:Lipopolysaccharides