title: Immunoactivation enhances the concentration of luteinizing hormone-releasing hormone peptide and its gene expression in human peripheral T-lymphocytes.
abstract: An immunomodulatory role for LHRH was suggested when we reported the presence of immunoactive and bioactive LHRH and its mRNA in rat splenic and thymic lymphocytes. In this paper we report that human peripheral T-cells as well as its subsets CD4+ and CD8+ contained immunoactive and bioactive LHRH. Furthermore, analysis of phytohemagglutinin (PHA)-activated T-cell lysates for LHRH by RIA demonstrated that the mean concentration of LHRH in PHA-activated T-cells increased from 45 +/- 4.5 to 64 +/- 7 pg/10(6) cells after 24 h of culture and from 47 +/- 3.6 to 117 +/- 11.8 pg/10(6) cells (P < 0.01) after 48 h. While the LHRH concentration in PHA-activated cells increased over the last 24 h of culture h from 64 +/- 7 to 117 +/- 11.8 pg/10(6) cells (P < 0.001), there was no change in mean concentration of LHRH in T-cells kept in medium alone. In a preliminary study we found that fresh T-cells contain 20 +/- 1.4 pg pro-LHRH/10(6) cells, and PHA stimulation increased the pro-LHRH content similar to the increase in LHRH. As with unfractionated T-cells, a significant PHA-induced time-dependent enhancement of intracellular LHRH was noted in CD4+ and CD8+ T-cells. RNA extracted from lymphocytes was subjected to reverse transcription-polymerase chain reaction analysis using LHRH and histone-3.3, primers, the latter as an internal control. The polymerase chain reaction-generated data demonstrated that the relative amount of LHRH mRNA in cultured, but non-PHA-stimulated (resting), cells diminished dramatically between 5-24 h, but recovered by 48 h of culture. The relative amount of LHRH mRNA in PHA-stimulated cells revealed a markedly different pattern. LHRH message expression in PHA-activated cells increased slightly at 5 h of culture and was maximally stimulated by 24 h, but declined by 48 h of culture. The PHA activation-induced time-dependent enhancement of intracellular accumulation of LHRH peptide at 5 and 24 h was accompanied by increased LHRH message. However, the increased concentration of LHRH peptide at 48 h coincided with decreased LHRH message expression. The data from total protein synthesis in PHA-activated cells showed a progressive increase in protein synthesis, a pattern entirely similar to the changes in the cell content of LHRH.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Research, Department of Veterans Affairs, Edward Hines Jr. Hospital, Hines, Illinois 60141.
authors.forename: N
authors.initials: N
authors.lastname: Azad
authors.forename: N
authors.initials: N
authors.lastname: La Paglia
authors.forename: K A
authors.initials: KA
authors.lastname: Jurgens
authors.forename: L
authors.initials: L
authors.lastname: Kirsteins
authors.forename: N V
authors.initials: NV
authors.lastname: Emanuele
authors.forename: M R
authors.initials: MR
authors.lastname: Kelley
authors.forename: A M
authors.initials: AM
authors.lastname: Lawrence
authors.forename: N
authors.initials: N
authors.lastname: Mohagheghpour
mesh_terms: D000328:Adult; D000368:Aged; D000906:Antibodies; D001483:Base Sequence; D017252:CD3 Complex / Q000276:immunology; D015870:Gene Expression; D007987:Gonadotropin-Releasing Hormone / Q000097:blood* / Q000235:genetics; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000097:blood; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000906:Antibodies; D017252:CD3 Complex; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone