Lectin histochemistry of normal and neoplastic peripheral nerve sheath. 2. Lectin binding patterns of schwannoma and neurofibroma.
Lectin binding patterns of 31 schwannomas and 6 neurofibromas were examined using 12 lectins, and the results were compared with those of normal peripheral nerves. Tumors obtained from 10 cases of neurofibromatosis and 4 recurrent schwannomas were included. Changes of glycoconjugates were observed in association with a neoplastic transformation of Schwann cells; Arachis hypogaea (PNA) staining after neuraminidase treatment seen in normal Schwann cells was reduced in schwannoma of Antoni type A, and bindings with Glycine max (SBA) and Helix pomatia (HPA) after sialic acid removal, which were not seen in normal Schwann cells, appeared in schwannoma cells. Intensities of staining of tumor cells with each lectin were higher in Antoni type B than those in Antoni type A. No differences in lectin binding patterns were observed between schwannomas in patients with neurofibromatosis or recurrent schwannomas and ordinary, primary schwannomas in patients without stigmata of neurofibromatosis. Lectin binding patterns of Schwann cells and perineurial cells in neurofibroma were almost similar to those in normal peripheral nerves with an exception of faint stain of Schwann cells with HPA after neuraminidase pretreatment. This result suggests differences in extent of differentiation between schwannoma cells and neoplastic Schwann cells in neurofibroma. Specific PNA binding to perineurial cells in neurofibroma indicates the significance of this lectin as a marker of these cells.
Matsumura
K
K
Department of Neurosurgery, Shiga University of Medical Science, Japan.
Nakasu
S
S
Nioka
H
H
Handa
J
J
D006001:Glycoconjugates / Q000032:analysis; D006801:Humans; D037102:Lectins; D009186:Myelin Sheath / Q000473:pathology* / Q000648:ultrastructure*; D009442:Neurilemmoma / Q000473:pathology*; D009455:Neurofibroma / Q000473:pathology*; D010525:Peripheral Nerves / Q000166:cytology* / Q000473:pathology*; D010524:Peripheral Nervous System Neoplasms / Q000473:pathology*; D012583:Schwann Cells / Q000166:cytology / Q000473:pathology
D006001:Glycoconjugates; D037102:Lectins