Regulation of Bacillus subtilis senS by homologous regulatory regions of senS and the inducible cat gene.
We have identified a stem-and-loop region between the promoter and open reading frame of the Bacillus subtilis senS gene containing a 9 base sequence (TTTAGATAA) identical to a sequence found in a similar regulatory element of the inducible pC194 cat gene. A high copy number of this pC194 regulatory region repressed the expression of senS, indicating that the stem-and-loop region of the cat gene titrated a positive factor necessary for senS expression. These results suggest that factors with similar binding requirements control senS and cat expression. In addition, senS was found to be autoregulated.
McCready
P
P
Department of Biochemistry and Biophysics, University of California, Davis 95616.
Takagi
M
M
Doi
R H
RH
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics*; D004790:Enzyme Induction; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D015500:Chloramphenicol O-Acetyltransferase