[Basic and clinical studies of serum CA195 antigen assay with "BL-CA195" kit].
We performed basic and clinical studies of IRMA "BL-CA195" kit in which monoclonal antibody CC3C195 was used as 125I-labeled tracer and solid phase antibody. The reproducibility of the assay results and dilution curves were satisfactory. There was a close correlation between serum CA195 and CA19-9 values, and many patients with pancreatic and colorectal cancer had elevated serum CA195 concentrations. Unlabeled CC3C195 antibody dose-dependently and completely inhibited the binding of 125I-labeled anti-CA19-9 antibody to its corresponding antigen. These findings suggest that CA195 and CA19-9 share common antigenic determinants.
Kobayashi
M
M
Department of Nuclear Medicine, Gunma University School of Medicine, Maebashi.
Yamaguchi
A
A
Imai
T
T
Otake
H
H
Igarashi
H
H
Hatori
N
N
Tomiyoshi
K
K
Inoue
T
T
Endo
K
K
Sakahara
H
H
D000328:Adult; D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis*; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; C081241:CA 195 antigen; D011933:Reagent Kits, Diagnostic