title: Rodent model of renal ischemia and reperfusion injury: influence of body temperature, seasonal variation, tumor necrosis factor, endogenous and exogenous antioxidants.
abstract: The influences of body temperature (T), seasonal variation, antiserum of tumor necrosis factor (TNF), exogenous and endogenous antioxidants on the extent of renal injury in a rodent model were examined. Renal injury was induced by 15-45 min of renal vascular (arterial and venous) occlusion followed by 1-3 days of reperfusion in right-nephrectomized male rats. Plasma creatinine concentration (PC, a reflection of renal injury) was independent of T when T was within 35-40 degrees C (n = 8), but directly related to T when T was within 31-35 degrees C (n = 10). PCs in rats with renal insults performed during October, November and December were similar (600 mcM, n = 10-24), but were higher than that during April (380 mcM, n = 16). Neither TNF antiserum nor exogenous antioxidants (Trolox, ascorbic acid, phytic acid, U-78517F) reduce PC (n = 5-7). However, PC was lower in normal rats than vitamin E deficient rats when subjected to 45 min (329.1 +/- 93.6 mcM vs. 695.9 +/- 37.7 mcM, n = 5-6), but not to 15-30 min of ischemia. In conclusion, renal injury induced by ischemia/reperfusion was T- and season-dependent. An excess of exogenous antioxidants did not reduce, whereas a reduction in endogenous antioxidant vitamin E level enhanced, renal injury.
authors.affiliation: Department of Cardiovascular Pharmacology, Sterling Winthrop Pharmaceuticals Research Group, Rensselaer, NY.
authors.forename: K C
authors.initials: KC
authors.lastname: Lee
authors.forename: D W
authors.initials: DW
authors.lastname: Hamel
authors.forename: S
authors.initials: S
authors.lastname: Kunbel
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001831:Body Temperature / Q000502:physiology*; D003404:Creatinine / Q000097:blood; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation / Q000502:physiology*; D015427:Reperfusion Injury / Q000503:physiopathology*; D012621:Seasons; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000503:physiopathology
chemical_list: D000975:Antioxidants; D014409:Tumor Necrosis Factor-alpha; D003404:Creatinine