title: Gene replacement and ectopic integration in the zygomycete Mucor circinelloides.
abstract: Transformation of Mucor circinelloides with plasmid pTL42 led to the identification of different integrative events. Analysis of eight transformed strains allowed the detection of three classes of transformants: integrative types derived from either homologous (exclusively at the leuA locus) or heterologous recombination and a class which originated by gene replacement. No autonomous replication of the vector was observed.
authors.affiliation: Department of Physiology, Carlsberg Laboratory, Copenhagen, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Arnau
authors.forename: P
authors.initials: P
authors.lastname: Strøman
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D005822:Genetic Vectors; D009089:Mucor / Q000235:genetics*; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014170:Transformation, Genetic