Enhanced response to daunomycin of normal, tumor and metastatic cell lines via drug photoactivation.
We have compared the cytotoxicity of daunomycin in vitro to highly differentiated normal epithelial cells (Fisher rat thyroid cells, FRTL-5) and to two neoplastic cell lines, a thyroid carcinoma (TK-6) and its lung metastasis (MPTK-6). Whereas the cell lines are equally sensitive to the drug in the dark, if irradiated during incubation with daunomycin (86 J/cm2 at 488 nm), they become more and differently sensitive. Namely, the drug doses producing 50% mortality decrease by factors of about 22, 28 and 16 for FRTL-5, TK-6 and MPTK-6 cell lines, respectively. This result correlates with differences in drug uptake and resistance observed in the normal and neoplastic cell lines.
Andreoni
A
A
C.N.R., Centro Endocrinologia ed Oncologia Sperimentale (C.E.O.S.), Napoli, Italy.
Colasanti
A
A
Kisslinger
A
A
Mastrocinque
M
M
Portella
G
G
Riccio
P
P
Roberti
G
G
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D003624:Darkness; D003630:Daunorubicin / Q000378:metabolism / Q000528:radiation effects* / Q000633:toxicity*; D008027:Light; D008175:Lung Neoplasms / Q000556:secondary; D051381:Rats; D011916:Rats, Inbred F344; D013961:Thyroid Gland; D013964:Thyroid Neoplasms; D014407:Tumor Cells, Cultured
D003630:Daunorubicin