title: Microsatellite polymorphisms for chromosome 5 bands q11.2-q13.3.
abstract: The genes for spinal muscular atrophy (SMA) and a possible subtype of schizophrenia (SCZD1) have been mapped to chromosome 5q11.2-q13.3. DNA markers have been mapped to 5q11.2-q13.3 using a hybrid cell line deleted for this region [Gilliam et al., Genomics 1989;5:940-944]. Genomic lambda clones for these markers facilitated the identification of highly polymorphic microsatellites. A total of ten microsatellites were identified and sequenced. Of these, seven were found to be polymorphic. Four had polymorphism information content values > 0.7. New polymorphic microsatellites were sequenced for D5S76, D5S125, D5S39, D5S127 and HEX-B. Two-point and multipoint analysis in non-CEPH pedigrees confirmed that the microsatellites were in tight linkage with each other. These new microsatellites will increase the efficiency of linkage analysis for these disorders.
authors.affiliation: Academic Department of Psychiatry, University College and Middlesex, School of Medicine, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: Sherrington
authors.forename: B
authors.initials: B
authors.lastname: Mankoo
authors.forename: M
authors.initials: M
authors.lastname: Dixon
authors.forename: D
authors.initials: D
authors.lastname: Curtis
authors.forename: G
authors.initials: G
authors.lastname: Kalsi
authors.forename: G
authors.initials: G
authors.lastname: Melmer
authors.forename: H
authors.initials: H
authors.lastname: Gurling
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000737:chemistry*; D005787:Gene Frequency; D015723:Gene Library; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009134:Muscular Atrophy, Spinal / Q000235:genetics*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012559:Schizophrenia / Q000235:genetics*
chemical_list: D004276:DNA, Satellite; D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides