title: C-type natriuretic peptide (CNP) is the major natriuretic peptide in human cerebrospinal fluid.
abstract: In order to investigate whether C-type natriuretic peptide (CNP) is present in human cerebrospinal fluid (CSF), we measured CNP-like immunoreactivity (-LI) in human CSF by specific radioimmunoassay (RIA) for CNP. We also measured atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) concentrations in human CSF. ANP-LI, BNP-LI, and CNP-LI concentrations of CSF collected from fifteen patients without neurological disorders were 0.20 +/- 0.13, 0.27 +/- 0.10, and 2.13 +/- 0.27 fmol/ml (mean +/- S.D.), respectively. In fifteen patients with neurological disorders, ANP-LI, BNP-LI, and CNP-LI concentrations in CSF were 0.21 +/- 0.18, 0.33 +/- 0.19, and 2.09 +/- 0.82 fmol/ml, respectively. Although ANP-LI and BNP-LI concentrations in plasma were much higher than those in CSF, CNP-LI was undetectable in plasma (less than 0.2 fmol/ml). These results demonstrate that three natriuretic peptides are present in CSF and that CNP is the major natriuretic peptide in human CSF. These results suggest that CNP in CSF is originated from and play important roles in the central nervous system.
authors.affiliation: Department of Neurosurgery, Kokura Memorial Hospital, Kitakyushu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: G
authors.initials: G
authors.lastname: Shirakami
authors.forename: K
authors.initials: K
authors.lastname: Nakao
authors.forename: I
authors.initials: I
authors.lastname: Nagata
authors.forename: O
authors.initials: O
authors.lastname: Nakagawa
authors.forename: N
authors.initials: N
authors.lastname: Hama
authors.forename: S
authors.initials: S
authors.lastname: Suga
authors.forename: S
authors.initials: S
authors.lastname: Miyamoto
authors.forename: H
authors.initials: H
authors.lastname: Kubo
authors.forename: O
authors.initials: O
authors.lastname: Hirai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000134:cerebrospinal fluid; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009318:Natriuresis / Q000502:physiology*; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000097:blood / Q000134:cerebrospinal fluid* / Q000502:physiology; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid; D011863:Radioimmunoassay
chemical_list: D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor