Complete nucleotide sequence of the 5-exo-hydroxycamphor dehydrogenase gene on the CAM plasmid of Pseudomonas putida (ATCC 17453).
We determined the complete nucleotide sequence of the first gene of Pseudomonas putida cytochrome P-450cam hydroxylase operon, camD, which encodes 5-exo-hydroxycamphor dehydrogenase. This dehydrogenase apparently consists of 361 amino acids and has a molecular mass of 38.4 kDa. Structural relationships to other zinc-containing alcohol dehydrogenases also became evident.
Aramaki
H
H
Department of Microbiology, Daiichi College of Pharmaceutical Sciences, Fukuoka, Japan.
Koga
H
H
Sagara
Y
Y
Hosoi
M
M
Horiuchi
T
T
D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D019475:Camphor 5-Monooxygenase; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016958:Pseudomonas putida / Q000235:genetics*; D016415:Sequence Alignment
D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000426:Alcohol Dehydrogenase; D019475:Camphor 5-Monooxygenase