Purine metabolism by intracellular Chlamydia psittaci.
Purine metabolism was studied in the obligate intracellular bacterium Chlamydia psittaci AA Mp in the wild type and a variety of mutant host cell lines with well-defined deficiencies in purine metabolism. C. psittaci AA Mp cannot synthesize purines de novo, as assessed by its inability to incorporate exogenous glycine into nucleic acid purines. C. psittaci AA Mp can take ATP and GTP, but not dATP or dGTP, directly from the host cell. Exogenous hypoxanthine and inosine were not utilized by the parasite. In contrast, exogenous adenine, adenosine, and guanine were directly salvaged by C. psittaci AA Mp. Crude extract prepared from highly purified C. psittaci AA Mp reticulate bodies contained adenine and guanine but no hypoxanthine phosphoribosyltransferase activity. Adenosine kinase activity was detected, but guanosine kinase activity was not. There was no competition for incorporation into nucleic acid between adenine and guanine, and high-performance liquid chromatography profiles of radiolabelled nucleic acid nucleobases indicated that adenine, adenosine, and deoxyadenosine were incorporated only into adenine and that guanine, guanosine, and deoxyguanosine were incorporated only into guanine. Thus, there is no interconversion of nucleotides. Deoxyadenosine and deoxyguanosine were cleaved to adenine and guanine before being utilized, and purine (deoxy)nucleoside phosphorylase activity was present in reticulate body extract.
McClarty
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Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Fan
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H
D000225:Adenine / Q000378:metabolism; D000818:Animals; D002460:Cell Line / Q000382:microbiology; D002691:Chlamydophila psittaci / Q000187:drug effects / Q000378:metabolism*; D006224:Cricetinae; D006147:Guanine / Q000378:metabolism; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D009696:Nucleic Acids / Q000378:metabolism; D015649:Pentostatin / Q000494:pharmacology; D011685:Purine Nucleotides / Q000378:metabolism; D011687:Purines / Q000378:metabolism*
D009696:Nucleic Acids; D011685:Purine Nucleotides; D011687:Purines; D006151:Guanosine; C032533:8-aminoguanosine; D015649:Pentostatin; D006147:Guanine; D000225:Adenine