title: A morphometrical study of the postnatal development of rat pancreatic islets, with special regard to the differences between Wistar and Sprague-Dawley strains.
abstract: Postnatal development of islet cells was morphometrically studied during neonatal (0, 1, 3, 5, and 7 days post partum << p.p. >>) and adult periods in Wistar and Sprague-Dawley rats, using two sensitive immunostainings, immunogold silver and protein A gold silver techniques. Nonfasted and fasted plasma glucose levels were also measured in adults. In addition, the frequency of developing type 2 diabetes was surveyed following injection of streptozotocin to neonates (2 days p.p.) of Wistar and Sprague-Dawley rats. The following results were obtained: 1) Islets grew more rapidly in Wistar than in Sprague-Dawley rats; 2) Increased percent areas of A and D cells and decreased percent area of B cells occurred during neonatal period in both Wistar and Sprague-Dawley rats; 3) Higher percent area of A cells and lower percent area of B cells were observed in Sprague-Dawley than in Wistar rats; 4) The duodenal pancreas of Wistar rats exhibited a marked neonatal increase in the percent area of pancreatic polypeptide (PP) cells and; 5) Sprague-Dawley rats were more susceptible to streptozotocin-induced type 2 diabetes, as compared with Wistar rats. An assumption is proposed that the differences in islet cell development between these rat strains are reflected in diabetes morbidity data.
authors.affiliation: Department of Anatomy, Oita Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ojiro
authors.forename: H
authors.initials: H
authors.lastname: Kitamura
authors.forename: T
authors.initials: T
authors.lastname: Shimada
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003921:Diabetes Mellitus, Experimental; D004198:Disease Susceptibility; D007515:Islets of Langerhans / Q000166:cytology / Q000254:growth & development* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000033:anatomy & histology*; D017208:Rats, Wistar / Q000033:anatomy & histology*; D016622:Silver Staining; D013311:Streptozocin
chemical_list: D013311:Streptozocin