title: Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate.
abstract: The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385-8391).
authors.affiliation: Laboratoire de Biochimie Microbienne, Université Claude Bernard, Villeurbanne, France.
authors.forename: M
authors.initials: M
authors.lastname: Malissard
authors.forename: C
authors.initials: C
authors.lastname: Duez
authors.forename: M
authors.initials: M
authors.lastname: Guinand
authors.forename: M J
authors.initials: MJ
authors.lastname: Vacheron
authors.forename: G
authors.initials: G
authors.lastname: Michel
authors.forename: N
authors.initials: N
authors.lastname: Marty
authors.forename: B
authors.initials: B
authors.lastname: Joris
authors.forename: I
authors.initials: I
authors.lastname: Thamm
authors.forename: J M
authors.initials: JM
authors.lastname: Ghuysen
mesh_terms: D000464:Alginates / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008969:Molecular Sequence Data; D010957:Plasmids; D011133:Polysaccharide-Lyases / Q000737:chemistry / Q000235:genetics*; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D011993:Recombinant Fusion Proteins; D017422:Sequence Analysis, DNA
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006603:Hexuronic Acids; D011135:Polysaccharides, Bacterial; D011993:Recombinant Fusion Proteins; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase