Envelope glycoproteins of HIV-1, HIV-2, and SIV purified with Galanthus nivalis agglutinin induce strong immune responses.
Lectin affinity chromatography was used to purify in a single step the envelope glycoproteins of HIV-1, HIV-2, and SIV. Envelope glycoproteins carry the major determinants essential for protection by the humoral immune response. The purification of these proteins has previously been a laborious procedure. The glycoproteins were purified by a one-step procedure to a high level of purity by using Galanthus nivalis agglutinin (GNA). The purified glycoprotein had CD4-binding and antigenic reactivities. Strong immune responses to envelope proteins and peptides were seen in mice and primates after immunization with these preparations.
Gilljam
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Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000302:isolation & purification; D015704:CD4 Antigens; D027801:Galanthus; D015686:Gene Products, env / Q000276:immunology / Q000302:isolation & purification*; D015483:HIV Antibodies / Q000096:biosynthesis; D015488:HIV Antigens / Q000302:isolation & purification; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D037102:Lectins; D008252:Macaca fascicularis; D037241:Mannose-Binding Lectins; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D037121:Plant Lectins; D015302:Simian Immunodeficiency Virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015704:CD4 Antigens; D015686:Gene Products, env; D015483:HIV Antibodies; D015488:HIV Antigens; D037102:Lectins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C063117:snowdrop lectin