A novel monoclonal antibody specifically reactive with human T-lymphotropic virus type-II (HTLV-II) envelope protein.
A novel monoclonal antibody (MAb), N5.4.4, specifically reactive with human T-lymphotropic virus type-II (HTLV-II) envelope glycoprotein (gp) has been developed through immunization with a synthetic peptide corresponding to the amino acid sequence 171-196 of the HTLV-II envelope protein (II-env 171-196). This MAb, which belonged to the IgG1 kappa subclass, reacted with the cytosmears of HTLV-II-infected cell lines (Si-IIA, CR-IIA-I and AS-IIA), but not with those of HTLV-I-infected cell lines (MT-1 and MT-2) or other HTLV-uninfected cell lines. On Western blot analysis, this MAb reacted with gp46 of HTLV-II lysates but not with HTLV-1 lysates. Moreover, flow-cytometric analysis revealed that this MAb recognized the native surface of only the HTLV-II-producing cells. Through application to immunohistochemical or serological method, this MAb may be of value in elucidating the pathogenesis of HTLV-II infection in comparison with HTLV-I.
Ohara
N
N
Second Department of Pathology, Okayama University Medical School, Japan.
Hayashi
K
K
Takahashi
K
K
Miyamoto
K
K
Akagi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D015153:Blotting, Western; D005434:Flow Cytometry; D015487:HTLV-II Antigens / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015487:HTLV-II Antigens; D007145:Immunoglobulin kappa-Chains; D014759:Viral Envelope Proteins