Production of interleukin-1 alpha and interleukin-2 by separate, phenotypically different leukaemia and human T cell lymphotropic virus-1-transformed T cell clones.
The relationship between interleukin-1 (IL-1) and interleukin-2 (IL-2) production and immunophenotype marker profiles was studied in a panel of 29 leukaemia and human T cell lymphotropic virus-1 (HTLV-1)-transformed T cell lines. Culture supernatants from six of the 29 T cell lines tested increased IL-2 production by the MOLT-16 cell line in a manner similar to that of rIL-1 alpha or rIL-1 beta. The enhancing activity in the cell culture supernatants was inhibited by antibody against IL-1 alpha. Anti-IL-1 beta antibody had no inhibitory effect. All the cell lines producing IL-1 alpha had characteristics of activated mature T cells. They were terminal deoxyribonucleotidyl transferase (TdT)-, CD4+, CD8-, HLA-DR+ and all were strongly positive for IL-2R alpha (Tac antigen) expression. However, none of the IL-1 alpha producing cell lines secreted detectable IL-2. A significant quantity of IL-2 was found, after stimulation with phytohaemagglutinin, in supernatants from nine of the 29 cell lines tested. The majority of IL-2 producing cell lines originated from less mature, non-activated T cells, as they were characterized by the expression of TdT, lack of HLA-DR antigens and > 50% had no detectable IL-2R alpha. The results thus show that separate, phenotypically different leukaemia and HTLV-1-transformed T cell clones produce IL-1 alpha and IL-2.(ABSTRACT TRUNCATED AT 250 WORDS)
Holán
V
V
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.
Minowada
J
J
D000906:Antibodies / Q000276:immunology; D002461:Cell Line, Transformed; D017077:Culture Media, Conditioned; D004253:DNA Nucleotidylexotransferase / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015368:Human T-lymphotropic virus 1 / Q000502:physiology; D006801:Humans; D016130:Immunophenotyping; D016898:Interferon-alpha / Q000276:immunology; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000032:analysis / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D009500:Neutralization Tests; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000906:Antibodies; D017077:Culture Media, Conditioned; D016898:Interferon-alpha; D007375:Interleukin-1; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D004253:DNA Nucleotidylexotransferase