The inhibition of human duodenal adenylate cyclase activity by Ca2+ and the effects of EGTA.
This study demonstrates that the inhibition of adenylate cyclase activity by Ca2+ is enhanced in the presence of increasing [EGTA] (0, 0.3, 1, 2.5 mM) by 2 orders of magnitude. It has been established that this effect is not because of poor Ca2+ buffering by low [EGTA] or high Ca2+ binding by the membrane preparation. It is present irrespective of stimulus. We suggest the enhanced sensitivity of adenylate cyclase to Ca2+ induced by EGTA is caused by the Ca-EGTA complex being a more inhibitory species than Ca2+. Thus consideration of the effects of the Ca-EGTA complex should be made when interpreting the results from experiments involving Ca2+ and EGTA.
Smith
J A
JA
Medical Research Centre, City Hospital, Nottingham, UK.
Griffin
M
M
Mireylees
S E
SE
Long
R G
RG
D000067956:Adenylyl Cyclase Inhibitors; D002118:Calcium / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology; D004357:Drug Synergism; D004386:Duodenum / Q000201:enzymology*; D004533:Egtazic Acid / Q000494:pharmacology*; D006801:Humans
D000067956:Adenylyl Cyclase Inhibitors; D005576:Colforsin; D004533:Egtazic Acid; D002118:Calcium