title: [Prognostic value of nuclear DNA of 106 colorectal tumors, determined with microspectrocytophotometry].
abstract: OBJECTIVE
  The aim of this study was to look for correlations between nuclear DNA content of colo-rectal tumors, and such clinical parameters as age, sex, location, CEA, histological grade and Duke's stages.
  
  
  METHODS
  A prospective study is carried out on surgical patients, subjected to standard criteria of radicality. Nuclear DNA content was quantified in tumoral cells by microcytophotometric techniques.
  
  
  METHODS
  106 patients with colo-rectal cancer. Patients with colonic perforation, other concomitant neoplasia, non-curative surgery or receiving adjuvant therapies were excluded from the study. Five patients died during the postoperative period and one was lost.
  
  
  RESULTS
  Histological grade: 28% G1, 35% G2 and 37% G3. Dukes': 8% A, 40% B, 32% C and 20% D. DNA quantification has rendered 45% as euploid and 55% as aneuploid. There was no statistical correlation between ploidy and location, age, sex or CEA. However, there is a clear preponderance of euploid tumors in G1 (23 vs. 5), while the aneuploid tumors double the euploid ones (24 and 25 vs. 12 and 12) in G2 and G3. A similar result was found comparing ploidy and Dukes: euploid tumors reach 77% both in stages A and B, while they drop to 24% and 14% in stages C and D. It has also been found that euploid tumors show a longer period of survival free of recurrence.
  
  
  CONCLUSIONS
  Evidence has been found supporting a prognostic value for tumoral DNA quantification in colo-rectal cancer. A longer follow-up is required to study absolute survival of the patients.
authors.affiliation: Departamento de Cirugía, Radiología y Medicina Física, Hospital de Cruces (Servicio de Cirugía A).
authors.forename: F J
authors.initials: FJ
authors.lastname: Díaz Aguirregoitia
authors.forename: I
authors.initials: I
authors.lastname: García-Alonso Montoya
authors.forename: I
authors.initials: I
authors.lastname: Iturburu Belmonte
authors.forename: V
authors.initials: V
authors.lastname: Portugal Porras
authors.forename: A
authors.initials: A
authors.lastname: Alonso Varona
authors.forename: A
authors.initials: A
authors.lastname: Echevarría García-Sanfrechoso
authors.forename: J
authors.initials: J
authors.lastname: Méndez Martín
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D003592:Cytophotometry; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D015591:Microspectrophotometry; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D011446:Prospective Studies; D012004:Rectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm
vernacular_title: Valor pronóstico del ADN nuclear de 106 tumores colorrectales, medido por microespectrocitofotometría.