title: Tomato golden mosaic virus leftward gene expression: autoregulation of geminivirus replication protein.
abstract: The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two DNA components, designated DNA A and DNA B. DNA A encodes AL1, the only viral protein required for DNA replication. AL1 protein interacts specifically with sequences in the common region that is conserved between the two genome components, near sequences involved in the transcription of complementary sense genes encoding BL1 protein and the AL1 protein itself. In the experiments described here, we replaced the AL1 and BL1 open reading frames with the beta-glucuronidase (GUS) reporter gene and used the gene replacement constructs to examine AL1 and BL1 gene expression in tobacco protoplasts. We found that expression of the GUS reporter in the AL1 replacement construct was reduced to background levels when transfections included a plasmid expressing AL1 protein from the cauliflower mosaic virus 35S promoter, indicating that AL1 gene expression is autoregulated. Surprisingly, a similar repression of BL1 gene expression by AL1 protein was not observed. Plasmids expressing the TGMV AL2 or AL3 proteins had no significant effect on AL1 or BL1 gene expression. In the course of these studies, we showed for the first time that the product of the AL3 ORF alone is sufficient to complement the replication-deficient phenotype of a TGMV AL3 mutant. The results are discussed in light of the multiple activities of AL1 protein.
authors.affiliation: Biotechnology Center, Ohio State University, Columbus 43210.
authors.forename: G
authors.initials: G
authors.lastname: Sunter
authors.forename: M D
authors.initials: MD
authors.lastname: Hartitz
authors.forename: D M
authors.initials: DM
authors.lastname: Bisaro
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis; D015967:Gene Expression Regulation, Viral; D005816:Genetic Complementation Test; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames; D010947:Plants, Toxic; D011523:Protoplasts; D014026:Nicotiana; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; C080288:replication protein AL1, Begomovirus; D005966:Glucuronidase