Determination of cisplatin and some possible metabolites by ion-pairing chromatography with inductively coupled plasma mass spectrometric detection.
A sensitive method is described for measuring cisplatin and some possible metabolites. The method combines reversed-phase ion-pairing liquid chromatography (LC) with inductively coupled plasma mass spectrometry (ICP-MS) for platinum-specific detection. Separation conditions for cisplatin hydrolysis products and the reaction products of cisplatin with methionine, cysteine, and glutathione have been investigated with sodium dodecylsulfate or heptanesulfonate as the ion-pairing agent. The detection limit for cisplatin was found to be 0.1 ng, corresponding to a concentration detection limit of 1 ng/ml when using an injection volume of 100 microliters. This study has demonstrated the usefulness of LC-ICP-MS for cisplatin metabolism studies.
Zhao
Z
Z
Department of Chemistry, University of Cincinnati, OH 45221-0172.
Tepperman
K
K
Dorsey
J G
JG
Elder
R C
RC
D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000032:analysis*; D003545:Cysteine / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D006868:Hydrolysis; D013058:Mass Spectrometry; D008715:Methionine / Q000032:analysis; D009944:Organoplatinum Compounds / Q000032:analysis; D013438:Sulfhydryl Compounds / Q000032:analysis
D009944:Organoplatinum Compounds; D013438:Sulfhydryl Compounds; D008715:Methionine; D005978:Glutathione; D003545:Cysteine; D002945:Cisplatin