New method for plague surveillance using polymerase chain reaction to detect Yersinia pestis in fleas.
Yersinia pestis, the plague bacillus, infects a variety of mammals throughout the world and is transmitted by fleas. We developed a polymerase chain reaction (PCR) test using primers designed from the Y. pestis plasminogen activator gene to directly detect plague-infected fleas. As few as 10 Y. pestis cells were detected, even in the presence of flea tissue, by PCR and then agarose gel electrophoresis and ethidium bromide staining. The feasibility of the assay was demonstrated by using naturally infected Xenopsylla cheopis fleas. The detection of Y. pestis in fleas by PCR provides a rapid and sensitive way to monitor plaque in wild animal populations, allowing public health officials to better assess the potential risk of transmission to humans.
Hinnebusch
J
J
Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
Schwan
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D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D007303:Insect Vectors / Q000382:microbiology; D008969:Molecular Sequence Data; D010930:Plague / Q000175:diagnosis / Q000635:transmission*; D010960:Plasminogen Activators / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D005423:Siphonaptera / Q000382:microbiology*; D015010:Yersinia pestis / Q000235:genetics* / Q000302:isolation & purification*
D004269:DNA, Bacterial; D010960:Plasminogen Activators