title: Two-dimensional immunoelectrophoresis of histoplasmin and a purified polysaccharide-protein antigen of Histoplasma capsulatum.
abstract: Crude histoplasmin and a polysaccharide-protein complex (PPC-histo) antigens obtained from culture filtrates of Histoplasma capsulatum were analyzed by single and tandem two-dimensional immunoelectrophoresis (TD-IEP) using a rabbit hyperimmune anti-histoplasmin polyvalent serum. Single TD-IEP showed 14 arc precipitates for histoplasmin. Continuity of arcs 2, 6, and 7, and 9 and 10 was observed, suggesting a different polymeric configuration of the same antigen. This was also confirmed in tandem TD-IEP of histoplasmin with homologous (PPC-histo) and heterologous PPC's from Blastomyces dermatitidis, Paracoccidioides brasiliensis and Coccidioides immitis. Tandem TD-IEP of histoplasmin and PPC-histo displayed a similar antigenic pattern to histoplasmin alone, being arcs 1 and 3 more evident and apparently present only in histoplasmin and PPC-histo. Tandem TD-IEP showed common antigens among the other heterologous fungal purified antigens, and seems useful to observe the multiplicity of antigens present in fungal preparations and to identify those precipitates (arcs 1 and 3) that are predominant in the purified preparation.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicinia, Universidad Nacional Autónoma de México.
authors.forename: C
authors.initials: C
authors.lastname: Toriello
authors.forename: J A
authors.initials: JA
authors.lastname: Jiménez-Montiel
authors.forename: M R
authors.initials: MR
authors.lastname: Reyes-Montes
authors.forename: M L
authors.initials: ML
authors.lastname: Taylor
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D006658:Histoplasma / Q000737:chemistry*; D006659:Histoplasmin / Q000737:chemistry*; D007123:Immunoelectrophoresis, Two-Dimensional; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits
chemical_list: D000946:Antigens, Fungal; D005656:Fungal Proteins; D011134:Polysaccharides; D006659:Histoplasmin