High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases.
A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer-assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.
Zhao
R H
RH
National Chromatographic R.&A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences.
Li
B Y
BY
Chen
N
N
Zhang
Y K
YK
Wang
Z Y
ZY
Lu
P C
PC
D000097:Acetonitriles; D001647:Bile Acids and Salts / Q000097:blood*; D001660:Biliary Tract Diseases / Q000097:blood / Q000175:diagnosis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003937:Diagnosis, Differential; D006801:Humans; D008107:Liver Diseases / Q000097:blood / Q000175:diagnosis*; D012997:Solvents
D000097:Acetonitriles; D001647:Bile Acids and Salts; D012997:Solvents; C032159:acetonitrile