[The recording of maximal Ca-ATPase activity in intact human erythrocytes by using a Ca-selective electrode].
The method for recording Ca-ATPase activity in intact erythrocytes was devised. The parameters of Ca2+ efflux, mediated by Ca-ATPase were investigated in Ca(2+)-loaded red blood cells, using Ca(2+)-selective electrode. The red blood cell calcium concentration was modified using the ionophore A23187 in Ca(2+) medium. Recording Ca efflux from RBC may be used as a routine method for the differential diagnosis of patients with essential hypertension and other forms of cell pathology.
Maksimova
N V
NV
Petrov
V V
VV
D001692:Biological Transport; D001782:Blood Donors; D002118:Calcium / Q000097:blood*; D000252:Calcium-Transporting ATPases / Q000097:blood*; D004566:Electrodes; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods
D007202:Indicators and Reagents; D000252:Calcium-Transporting ATPases; D002118:Calcium
Registratsiia maksimal'noĭ aktivnosti Ca-ATFazy v intaktnykh éritrotsitakh cheloveka pri pomoshchi Ca-selektivnogo élektroda.