Venom of an aggressive African Theraphosidae (Scodra griseipes): milking the venom, a study of its toxicity and its characterization.
Two milking procedures, electric stimulation and lure-biting, are described and compared. The amount of crude Scodra griseipes venom collected in 3 years by lure-biting was 1670 mg and 1380 mg, respectively, for young females and adult males. The crude venom appears pure on the basis of UV-Vis spectra, which show no hemocyanin absorption bands. The toxicity and induced symptoms of these venoms in mice were evaluated by direct bites and also by LD50 [LD50 (young female venom, i.v.) = 8.1 mg/kg, LD50 (adult male venom, i.v.) > 9.5 mg/kg]. The venom from both sexes was compared by protein content, UV-Vis and 1H-NMR spectroscopies, polyacrylamide gel electrophoresis [SDS-PAGE] and size exclusion high performance liquid chromatography [SE-HPLC]. Differences were observed.
Celerier
M L
ML
Université P. et M. Curie, URA-CNRS 689, Paris, France.
Paris
C
C
Lange
C
C
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004558:Electric Stimulation; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006135:Gryllidae; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011506:Proteins / Q000032:analysis; D012727:Sex Characteristics; D013056:Spectrophotometry, Ultraviolet; D001098:Spider Bites / Q000473:pathology; D013111:Spider Venoms / Q000737:chemistry / Q000633:toxicity*; D013112:Spiders / Q000502:physiology*
D011506:Proteins; D013111:Spider Venoms