Vitamin E increases interleukin-2 dependent cellular growth and glycoprotein glycosylation in murine cytotoxic T-cell line.
The effect of vitamin E on the modulation of interleukin-2 (IL-2) dependent cellular growth, glycosylation, and expression of certain glycoproteins (GP) was examined in a murine cytotoxic T-cell line (CTLL). CTLL cultures in log phase and growing in 10% IL-2 medium in the presence of 1-16 micrograms vitamin E/ml showed a dose-dependent 2- to 15-fold stimulation of growth (assessed by 3H-thymidine incorporation) relative to controls. Vitamin E-treated cultures were observed to grow more efficiently at lower levels of IL-2 compared to controls. Glycoconjugate biosynthesis (assessed by 3H-galactose incorporation) was stimulated 5- to 15-fold by the addition of vitamin E to the culture medium. GP glycosylation as determined by wheat germ agglutinin binding GP (50-64 Kd) and concanavalin A binding GP (38-200 Kd) increased significantly in the presence of vitamin E. These results demonstrate that vitamin E stimulates IL-2 dependent cellular growth, glycosylation, and expression of certain GP in CTLL.
Gogu
S R
SR
Antioxidants Research Laboratory, Tufts University, Boston, MA.
Blumberg
J B
JB
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D005690:Galactose / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D007376:Interleukin-2 / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014810:Vitamin E / Q000494:pharmacology*
D006023:Glycoproteins; D007376:Interleukin-2; D014316:Tritium; D014810:Vitamin E; D004247:DNA; D013936:Thymidine; D005690:Galactose