Actinobacillus pleuropneumoniae haemolysin II is secreted from Escherichia coli by A. pleuropneumoniae pleurotoxin secretion gene products.
Actinobacillus pleuropneumoniae serotype 2 secretes type II haemolysin and pleurotoxin activities. Here, the genes for type II haemolysin were cloned in Escherichia coli, but type II haemolysin antigen and haemolysin activity were only detected intracellularly and not exported to culture supernatant. It has been reported that the genes for type II haemolysin are not linked to functional secretion genes, while those for pleurotoxin are. In this report the means of secretion of type II haemolysin was examined by constructing a hybrid plasmid carrying the genes required for type II haemolysin expression, together with determinants which allow secretion of pleurotoxin and are linked to the pleurotoxin toxin genes. These genes facilitated the export of type II haemolysin from E. coli, and may perform this function in A. pleuropneumoniae.
Macdonald
J
J
Department of Veterinary Pathology, University of Glasgow Veterinary School, UK.
Rycroft
A N
AN
D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D015183:Restriction Mapping
D001427:Bacterial Toxins; D002352:Carrier Proteins; D006460:Hemolysin Proteins