title: Proposed pathway for biosynthesis of the S-layer glycoprotein of Bacillus alvei.
abstract: The outer surface of the murein sacculus of the eubacterium Bacillus alvei is covered by a surface layer (S-layer) glycoprotein. The glycan chain of this S-layer glycoprotein consists of trisaccharide repeating units with ManNAc, Gal, and Glc as constituents. From cell extracts of B. alvei, nucleotide-activated derivatives of ManNAc, Gal, Glc, and GlcNAc were isolated. Furthermore, GDP- and dolichyl-activated oligosaccharides were obtained. On the basis of the isolated putative glycoprotein precursors, a pathway for the biosynthesis of the oligosaccharide chain is proposed.
authors.affiliation: Angewandte Mikrobiologie, Universität Ulm, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Hartmann
authors.forename: P
authors.initials: P
authors.lastname: Messner
authors.forename: G
authors.initials: G
authors.lastname: Allmeier
authors.forename: H
authors.initials: H
authors.lastname: König
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D001426:Bacterial Proteins; D002240:Carbohydrate Sequence; D002241:Carbohydrates; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004187:Disaccharides / Q000096:biosynthesis / Q000302:isolation & purification; D013058:Mass Spectrometry; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D009702:Nucleoside Diphosphate Sugars / Q000302:isolation & purification / Q000378:metabolism; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000378:metabolism; D014312:Trisaccharides / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002241:Carbohydrates; D004187:Disaccharides; D008562:Membrane Glycoproteins; D009702:Nucleoside Diphosphate Sugars; D011104:Polyisoprenyl Phosphate Oligosaccharides; C055764:S-layer proteins; D014312:Trisaccharides