Techniques for cloning cDNAs encoding interactive transcriptional regulatory proteins.
Several approaches aimed at detecting and cloning interactive transcriptional regulatory proteins have been presented. All of the techniques can effectively identify specific interactions between two transcription proteins. However, interaction cloning and the two hybrid system have the added advantage of yielding a cDNA expression clone directly. The other methods, EMSA-mediated cloning, co-immunoprecipitation, oligonucleotide/PCR-facilitated cloning, Southwestern, and Farwestern, require additional manipulations to obtain a cDNA clone. Clearly, the interactive cloning system of choice will depend on the proteins under investigation.
Silver
S C
SC
Department of Medicine, University of North Carolina, Chapel Hill 27599-7280.
Hunt
S W
SW
D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D014157:Transcription Factors; D004247:DNA