Binding of anti-H-Y monoclonal antibodies to separated X and Y chromosome-bearing porcine and bovine sperm.
Studies designed to answer the question whether or not H-Y antigen is preferentially expressed on Y chromosome bearing sperm have resulted in conflicting results. This is probably due to the absence of reliable methods for estimating the percentage of X and Y chromosome bearing sperm in fractions, enriched or depleted for H-Y antigen positive sperm. In recent years a reliable method for separating X and Y chromosome bearing sperm has been published. With this method, separation is achieved by using a flow cytometer/cell sorter, which detects differences in DNA content. This technique provided the first opportunity for testing anti-H-Y antibody binding to fractions enriched for X and Y chromosome bearing sperm, directly. A total of 7 anti-H-Y monoclonal antibodies were tested using sorted porcine sperm and in one experiment also sorted bovine sperm. All monoclonal antibodies bound only a fraction of the sperm (20 to 50%). However, no difference in binding to the X and Y sperm enriched fractions was found. Therefore, the present experiments do not yield evidence that H-Y antigen is preferentially expressed in Y chromosome bearing sperm.
Hendriksen
P J
PJ
Immunobiology Department, Research Institute for Animal Production (IVO-DLO) Schoonoord, Zeist, The Netherlands.
Tieman
M
M
Van der Lende
T
T
Johnson
L A
LA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D002417:Cattle / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006182:H-Y Antigen / Q000032:analysis* / Q000276:immunology; D008297:Male; D013094:Spermatozoa / Q000276:immunology* / Q000378:metabolism; D013552:Swine / Q000276:immunology*; D014960:X Chromosome; D014998:Y Chromosome
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen