title: Antibody response in bronchoalveolar lavage and bile in rats after aerosol immunisation with an Escherichia coli strain producing ovalbumin.
abstract: We studied the expression of the antibody response in two parts of the mucosal immune system after exposing rats to an aerosol containing an Escherichia coli O6 strain which carries type 1 fimbriae and which is genetically manipulated to produce ovalbumin (OA). For comparison rats were immunised twice in the Peyer's patches (PP), 3 weeks apart, with the same bacteria. Bile, serum and bronchoalveolar lavage (BAL) were collected from both groups and the levels of antibodies directed against O6 lipopolysaccharide (LPS), type 1 fimbriae and OA were determined. The aerosol-exposed animals all developed higher levels of IgA anti-LPS antibodies in BAL than in bile. IgA antifimbrial antibodies were not detected in BAL despite the presence of such antibodies in the bile. Thus, the ratio BAL:bile was much higher for the anti-LPS antibodies than for the antifimbrial antibodies. This was in contrast to the PP-immunised animals which developed both IgA antifimbrial as well as anti-LPS antibodies in BAL although at a lower level than in bile and the ratios BAL:bile for the antibodies against fimbriae and LPS were similar. IgA anti-OA antibodies were present in bile from both groups but not detected in BAL in any group. IgG anti-LPS antibodies were very high in BAL and in serum in the aerosol-immunised animals, while neither IgG antifimbrial nor anti-OA antibodies were detected. The PP-immunised animals on the other hand developed IgG antibodies against both the fimbrial and the LPS antigens in serum but only against fimbriae in BAL.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Clinical Immunology, University of Göteborg, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Wiedermann
authors.forename: S
authors.initials: S
authors.lastname: Ahlstedt
authors.forename: B
authors.initials: B
authors.lastname: Andersson
authors.forename: U I
authors.initials: UI
authors.lastname: Dahlgren
mesh_terms: D000336:Aerosols; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D001646:Bile / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism; D007114:Immunization; D007070:Immunoglobulin A / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D009092:Mucous Membrane / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism*; D010581:Peyer's Patches / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000336:Aerosols; D000906:Antibodies; D000941:Antigens; D007070:Immunoglobulin A; D008070:Lipopolysaccharides; D010047:Ovalbumin