title: High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases.
abstract: A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer-assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.
authors.affiliation: National Chromatographic R.&A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences.
authors.forename: R H
authors.initials: RH
authors.lastname: Zhao
authors.forename: B Y
authors.initials: BY
authors.lastname: Li
authors.forename: N
authors.initials: N
authors.lastname: Chen
authors.forename: Y K
authors.initials: YK
authors.lastname: Zhang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.forename: P C
authors.initials: PC
authors.lastname: Lu
mesh_terms: D000097:Acetonitriles; D001647:Bile Acids and Salts / Q000097:blood*; D001660:Biliary Tract Diseases / Q000097:blood / Q000175:diagnosis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003937:Diagnosis, Differential; D006801:Humans; D008107:Liver Diseases / Q000097:blood / Q000175:diagnosis*; D012997:Solvents
chemical_list: D000097:Acetonitriles; D001647:Bile Acids and Salts; D012997:Solvents; C032159:acetonitrile