Calcium ionophore A23187 induces interleukin-8 gene expression and protein secretion in human monocytic cells.
The regulation of the expression of the interleukin-8 (IL-8) gene in human monocytic cell lines has been investigated. Agents such as interleukin-1 (IL-1) or interferon-gamma (IFN gamma) did not induce increased IL-8 expression in THP-1 or U937 cells. Bacterial lipopolysaccharide endotoxin (LPS) or phorbol myristate acetate (PMA) alone induced suboptimal expression as assessed by Northern blotting; however, preincubation of cells with PMA followed by endotoxin induced much higher levels of IL-8 mRNA. Incubation of the cells with the calcium ionophore A23187 resulted in consistent increased IL-8 gene expression comparable to that of cells treated with endotoxin alone. In addition to inducing IL-8 mRNA this calcium ionophore also induced IL-8 protein synthesis as assessed by immunofluorescence and secretion as detected by ELISA. These results indicate that increases in intracellular calcium result in IL-8 gene expression and protein secretion.
Wilson
L
L
Yamanouchi Research Institute, Littlemore Hospital, Oxford, UK.
Butcher
C J
CJ
Kellie
S
S
D015152:Blotting, Northern; D000001:Calcimycin / Q000494:pharmacology*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism
D016209:Interleukin-8; D000001:Calcimycin