Parasite lactate dehydrogenase as an assay for Plasmodium falciparum drug sensitivity.
This report compares the use of the lactate dehydrogenase (pLDH) assay with 3H-hypoxanthine incorporation and Giemsa microscopy for the evaluation of anti-malaria drug inhibition of the growth of P. falciparum in vitro. The inhibition profiles and IC50 determinations of the pLDH assay were directly comparable to those determined by the radioactive uptake and microscopic methods. Furthermore, the pLDH culture sensitivity assay is reproducible, easily interpreted, rapid and inexpensive to perform, suggesting field applicability.
Makler
M T
MT
Laboratory and Research Service, Veterans Affairs Medical Center, Portland, Oregon.
Ries
J M
JM
Williams
J A
JA
Bancroft
J E
JE
Piper
R C
RC
Gibbins
B L
BL
Hinrichs
D J
DJ
D000818:Animals; D001399:Azure Stains; D002738:Chloroquine / Q000494:pharmacology*; D003124:Colorimetry; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D012044:Regression Analysis; D015203:Reproducibility of Results
D001399:Azure Stains; D007042:Hypoxanthines; D019271:Hypoxanthine; D002738:Chloroquine; D007770:L-Lactate Dehydrogenase