The lysosomal gp63-related protein in Leishmania mexicana amastigotes is a soluble metalloproteinase with an acidic pH optimum.
Leishmania mexicana amastigotes express a lysosomal protein, which is antigenically related to the promastigote surface metalloproteinase (gp63). It is shown that the purified gp63-related protein from amastigote is also an active metalloproteinase. The pH-optimum of the enzyme is acidic, similar to lysosomal cysteine proteinases, but distinct from the neutral to basic pH-optimum of the promastigote surface proteinase. This study appears to be the first report on a metalloproteinase with a lysosomal localization.
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Max-Planck-Institut für Biologie, Tübingen, Germany.
Harbecke
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Overath
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D000143:Acids; D000818:Animals; D002850:Chromatography, Gel; D003546:Cysteine Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007894:Leishmania mexicana / Q000201:enzymology / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism; D008666:Metalloendopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility
D000143:Acids; D008562:Membrane Glycoproteins; D011506:Proteins; C018589:lysosomal proteins; D003546:Cysteine Endopeptidases; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania