Immunoassay of podophyllotoxin.
The purpose of our research was to design and develop a sensitive immunoassay for the natural Podophyllum lignan, podophyllotoxin [1], which is the precursor of the anti-cancer drugs etoposide [2] and teniposide [3]. In order to obtain a specific antiserum, bovine serum albumin and keyhole limpet hemocyanin conjugates of succinyl-podophyllotoxin [5] were prepared as antigenic hapten carrier proteins. These antigens were used to develop polyclonal antibodies to 1 in rabbits. The antisera were used for the development of an immunoassay for the detection of 1 and its 4'-demethyl derivative 4. As little as 0.16 nmol of 1 is detectable with this ELISA. The assay was applied to the direct analysis of 1 in plants for the screening of potential drug plants which allowed quantitation in less than 0.2 mg of plant tissue.
Yoo
K
K
Department of Chemistry, Philadelphia College of Pharmacy and Science, Pennsylvania 19104.
Porter
J R
JR
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D007106:Immune Sera / Q000737:chemistry; D011034:Podophyllotoxin / Q000032:analysis* / Q000276:immunology / Q000592:standards; D011817:Rabbits
D000972:Antineoplastic Agents, Phytogenic; D007106:Immune Sera; D011034:Podophyllotoxin