The identification of a naturally occurring cell surface growth inhibitor related to a previously described bovine sialoglycopeptide.
A 66-kDa sialoglycoprotein has been identified as the parental membrane molecule of an earlier described sialoglycopeptide (SGP), an 18-kDa molecule released by protease treatment of intact bovine cerebral cortex cells that was shown to be a potent inhibitor of cellular proliferation. The 66-kDa parental sialoglycoprotein (p-SGP) was purified approximately 2,400-fold, to apparent homogeneity, from bovine cerebral cortex cell membranes by its release during incubation with 3 M NaCl, preparative isoelectric focusing and lectin affinity chromatography. Although a membrane-associated molecule, the p-SGP appeared to be tightly bound to the cell membrane, since it was not released during incubations in the absence of 3 M NaCl. Incubation of the membrane preparations with 3 M urea proved to be too harsh, and the antigenicity required to follow the purification of the p-SGP was abolished. Analyses by SDS-PAGE, under reducing and nonreducing conditions, suggested that the p-SGP membrane component was a single polypeptide without subunit structure. The p-SGP was shown to be structurally related to the SGP fragment by immunoblots with IgG raised to the SGP inhibitor, and functionally related to the SGP by its ability to inhibit Swiss 3T3 proliferation at concentrations strikingly similar to that previous measured with the SGP fragment.
Fattaey
H K
HK
Center for Basic Cancer Research, Kansas State University, Manhattan 66506-4903.
Enebo
D J
DJ
Moos
P J
PJ
Johnson
T C
TC
D016475:3T3 Cells; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000737:chemistry; D006131:Growth Inhibitors / Q000032:analysis* / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D012795:Sialoglycoproteins / Q000032:analysis* / Q000494:pharmacology
D006131:Growth Inhibitors; D008562:Membrane Glycoproteins; D012795:Sialoglycoproteins