[Culture of human adult endothelial cells derived from cadavers of autopsy].
I cultured human adult endothelial cells derived from cadavers. Iliac arteries of cadavers were resected at autopsy and their endothelial cells were yielded sterile by digestion of 0.1% collagenase solution. I tried culture of endothelial cells of 72 cadavers at autopsy and 14 cases of them were successful. They were observed with phase-contrast microscopy, transmission electron microscopy and immunofluorescence microscopy, which showed polygonal monolayered cells, Weibel-Palade bodies and Factor VIII antigen in these cells. I also examined the doubling time (2-4 weeks) of these cells. 3H-Thymidine leveling index showed the differentiation of effects between fibroblast growth factor and endothelial cell growth factor on human adult endothelial cells, while no differentiation between them on human umbilical vein endothelial cells. Also, I observed apolipoprotein E in the cells, but not in umbilical vein endothelial cells. It established the procedure of their culture method.
Okumura
M
M
First Department of Surgery, School of Medicine, Tokyo University, Japan.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001057:Apolipoproteins E / Q000032:analysis; D002102:Cadaver; D002455:Cell Division; D002478:Cells, Cultured; D003584:Cytological Techniques; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005169:Factor VIII / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
D001057:Apolipoproteins E; D005169:Factor VIII