RNA-2 of tobacco rattle virus encodes the determinants of transmissibility by trichodorid vector nematodes.
Pseudorecombinant isolates produced from an efficiently transmitted and a non-transmitted isolate of tobacco rattle tobravirus were tested for their transmissibility by trichodorid vector nematodes. Isolates with RNA-2 originating from the non-transmissible isolate were not transmitted by the vector, whereas isolates with RNA-2 originating from the efficiently transmitted isolate were transmitted efficiently. It is therefore concluded that the factor determining vector transmissibility is located on the RNA-2 genome segment of TRV. Because the serological properties of the isolates also correlate with transmissibility, it is likely that the virus particle protein is involved in the transmission process.
Ploeg
A T
AT
Scottish Crop Research Institute, Invergowrie, Dundee, U.K.
Robinson
D J
DJ
Brown
D J
DJ
D000818:Animals; D004199:Disease Vectors; D009348:Nematoda; D010935:Plant Diseases / Q000382:microbiology; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D010947:Plants, Toxic; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic; D014026:Nicotiana / Q000382:microbiology*
D012367:RNA, Viral