The candidate proto-oncogene bcl-3 encodes a transcriptional coactivator that activates through NF-kappa B p50 homodimers.
The candidate proto-oncogene bcl-3 encodes a protein that shares structural features with I kappa B-alpha and other proteins that bind to members of the Rel protein family. Here, we show that in contrast to the inhibitory activity of I kappa B-alpha, the bcl-3 gene product superactivates NF-kappa B p50 homodimer-mediated gene expression both in vivo and in vitro. BCL-3 protein can, as well, selectively associate with p50 homodimers in the presence of DNA containing a kappa B motif. These results strongly suggest that BCL-3 can act as a transcriptional coactivator, acting through DNA-bound p50 homodimers.
Fujita
T
T
Rockefeller University, New York, New York 10021.
Nolan
G P
GP
Liou
H C
HC
Scott
M L
ML
Baltimore
D
D
D000818:Animals; D000081242:B-Cell Lymphoma 3 Protein; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators; D014157:Transcription Factors; D014158:Transcription, Genetic
D000081242:B-Cell Lymphoma 3 Protein; C000631344:BCL3 protein, human; C000631345:Bcl3 protein, mouse; C000715470:MAS1 protein, human; D016328:NF-kappa B; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D004247:DNA; D008156:Luciferases