title: Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein.
abstract: Tetanus toxin inhibits neurotransmitter release by selectively blocking fusion of synaptic vesicles. Recently tetanus toxin was shown to proteolytically degrade synaptobrevin II (also named VAMP-2), a synaptic vesicle-specific protein, in vitro and in nerve terminals. As targets of tetanus toxin, synaptobrevins probably function in the exocytotic fusion of synaptic vesicles. Here we describe a new synaptobrevin homologue, cellubrevin, that is present in all cells and tissues tested and demonstrate that it is a membrane trafficking protein of a constitutively recycling pathway. Like synaptobrevin II, cellubrevin is proteolysed by tetanus toxin light chain in vitro and after transfection. Our results suggest that constitutive and regulated vesicular pathways use homologous proteins for membrane trafficking, probably for membrane fusion at the plasma membrane, indicating a greater mechanistic and evolutionary similarity between these pathways than previously thought.
authors.affiliation: Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: H T
authors.initials: HT
authors.lastname: McMahon
authors.forename: Y A
authors.initials: YA
authors.lastname: Ushkaryov
authors.forename: L
authors.initials: L
authors.lastname: Edelmann
authors.forename: E
authors.initials: E
authors.lastname: Link
authors.forename: T
authors.initials: T
authors.lastname: Binz
authors.forename: H
authors.initials: H
authors.lastname: Niemann
authors.forename: R
authors.initials: R
authors.lastname: Jahn
authors.forename: T C
authors.initials: TC
authors.lastname: Südhof
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport / Q000502:physiology; D016466:CHO Cells; D006224:Cricetinae; D008561:Membrane Fusion / Q000502:physiology*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry; D016716:PC12 Cells; D050683:R-SNARE Proteins; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013572:Synaptic Vesicles / Q000502:physiology*; D016708:Synaptophysin / Q000032:analysis; D013744:Tetanus Toxin / Q000378:metabolism*; D051040:Vesicle-Associated Membrane Protein 3
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050683:R-SNARE Proteins; D016708:Synaptophysin; D013744:Tetanus Toxin; D051040:Vesicle-Associated Membrane Protein 3