B cell function in SCID mice reconstituted with allotype-disparate spleen and peritoneal cavity B cells.
Severe combined immune-defective (SCID) mice reconstituted with immunoglobulin heavy chain-disparate spleen (SP) and peritoneal cavity (PerC) B cells exhibit serologic dominance of IgM bearing the PerC allotype. Immunization fails to elicit IgM production from donor SP B cells although flow cytometric analyses indicate the presence of these cells in the spleen of (SP + PerC)-->SCID chimeras. This observation suggests that donor SP B cell function is absent or inhibited in the SCID chimera. In this report, ELISAspot and proliferation assays were employed to further investigate the functional properties of B cells in these mice. Plasma cells derived from the donor SP were present in the spleen of SCID recipients at a lower number than those derived from donor PerC B cells. Spleen cells isolated from SCID recipients could be activated with insolubilized MAbs directed against IgM or IgD allotypes expressed by either the SP or PerC B cell donor. In contrast, the peritoneal cavity of SCID chimeras lacked B cells responsive to MAbs specific for the donor SP IgM and IgD allotypes. These results demonstrate that B cells derived from the SP donor are functional in (SP + PerC)-->SCID chimeras.
Riggs
J E
JE
Department of Biology, Rider College, Lawrenceville, New Jersey 08648.
Dejneka
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured / Q000276:immunology; D002678:Chimera; D004195:Disease Models, Animal; D007072:Immunoglobulin D / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D016513:Mice, SCID; D010537:Peritoneum / Q000166:cytology / Q000276:immunology; D016511:Severe Combined Immunodeficiency / Q000276:immunology*; D013154:Spleen / Q000276:immunology* / Q000637:transplantation
D000911:Antibodies, Monoclonal; D007072:Immunoglobulin D; D007075:Immunoglobulin M