title: Cytokines in sputum and serum from patients with cystic fibrosis and chronic Pseudomonas aeruginosa infection as markers of destructive inflammation in the lungs.
abstract: The presence of interleukin (IL)-1 receptor antagonist (IRAP) in plasma and sputum from patients with cystic fibrosis (CF) and chronic Pseudomonas aeruginosa lung infection was investigated together with IL-1 alpha, IL-1 beta, IL-6, and tumor necrosis factor-alpha (TNF) in a cross-sectional study. All cytokines were assayed by ELISAs. High concentrations of IRAP, IL-1 alpha, IL-1 beta, IL-6, and TNF in sputum samples and low or nondetectable levels of circulating cytokines were frequently found. Increased concentrations of plasma IRAP are positively correlated with decreasing pulmonary function. In a longitudinal study of serum IRAP, a higher level of IRAP was detected in a group of patients with poor pulmonary function compared to a group with good pulmonary function.
authors.affiliation: Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
authors.forename: G
authors.initials: G
authors.lastname: Kronborg
authors.forename: M B
authors.initials: MB
authors.lastname: Hansen
authors.forename: M
authors.initials: M
authors.lastname: Svenson
authors.forename: A
authors.initials: A
authors.lastname: Fomsgaard
authors.forename: N
authors.initials: N
authors.lastname: Høiby
authors.forename: K
authors.initials: K
authors.lastname: Bendtzen
mesh_terms: D000293:Adolescent; D000328:Adult; D002908:Chronic Disease; D003430:Cross-Sectional Studies; D003550:Cystic Fibrosis / Q000150:complications / Q000276:immunology / Q000473:pathology*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D007249:Inflammation; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007378:Interleukins / Q000032:analysis*; D008137:Longitudinal Studies; D008168:Lung / Q000473:pathology; D008297:Male; D011552:Pseudomonas Infections / Q000150:complications* / Q000473:pathology; D012141:Respiratory Tract Infections / Q000150:complications* / Q000473:pathology; D012795:Sialoglycoproteins / Q000032:analysis*; D013183:Sputum / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D014797:Vital Capacity
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D012795:Sialoglycoproteins; D014409:Tumor Necrosis Factor-alpha