title: Alkylation damage and repair in Alzheimer's disease lymphocytes.
abstract: We have previously shown that lymphocytes from Alzheimer's disease (AD) patients are hypersensitive to ionising radiation, as revealed by their higher levels of dicentrics than those from age-matched, normal individuals. We have now examined the sensitivity of these cells to N-methyl-N-nitrosourea or methyl methanesulphonate, using a variety of assays, namely unscheduled DNA synthesis, viability, sister chromatid exchange and, via high performance liquid chromatography, level of methylation and repair of the adducts, 7-methylguanine, 0(6)-methylguanine and 3-methyladenine. We have found that AD lymphocytes and age-matched normal lymphocytes respond similarly to methylation damage, as assessed by all these techniques.
authors.affiliation: Department of Optometry and Vision Sciences, UMIST, Manchester, UK.
authors.forename: S E
authors.initials: SE
authors.lastname: Tobi
authors.forename: D
authors.initials: D
authors.lastname: Neary
authors.forename: R F
authors.initials: RF
authors.lastname: Itzhaki
mesh_terms: D000328:Adult; D000368:Aged; D000478:Alkylation; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D002452:Cell Count; D002470:Cell Survival; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008770:Methylnitrosourea / Q000633:toxicity; D008875:Middle Aged; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism
chemical_list: D008770:Methylnitrosourea; D004247:DNA; D008741:Methyl Methanesulfonate; D013936:Thymidine