Analysis of the structure and subcellular location of filamentous phage pIV.
The gene IV protein of filamentous bacteriophages is an integral membrane protein required for phage assembly and export. A series of gene IV::phoA fusion, gene IV deletion, and gene IV missense mutations have been isolated and characterized. The alkaline phosphatase activity of the fusion proteins suggests that pIV lacks a cytoplasmic domain. Cell fractionation studies indicate that the carboxy-terminal half of pIV mediates its assembly into the membrane, although there is no single, discrete membrane localization domain. The properties of gene IV missense and deletion mutants, combined with an analysis of the similarities between pIVs from various filamentous phage and related bacterial export-mediating proteins, suggest that the amino-terminal half of pIV consists of a periplasmic substrate-binding domain that confers specificity to the assembly-export system.
Russel
M
M
Rockefeller University, New York, New York 10021.
Kaźmierczak
B
B
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis; D002451:Cell Compartmentation; D003090:Coliphages / Q000235:genetics / Q000254:growth & development*; D004252:DNA Mutational Analysis; D015964:Gene Expression Regulation, Bacterial; D005814:Genes, Viral / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D009876:Operon / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013104:Spheroplasts / Q000378:metabolism; D013347:Subcellular Fractions; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D014779:Virus Replication
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C069816:phage shock protein, Bacteria; D000469:Alkaline Phosphatase