[Application of cyclosporin for cornea graft].
This study aimed to determine the optimal concentration rate of clinically effective Cyclosporin (CYA). Exchange penetrating keratoplasties were performed on 30 albino rabbits divided into 3 groups of ten, Groups I, II, and III, using 7 mm. trephine. In Group I 0.025% CYA drops were administered 4 times per day for 50 days and similarly 0.05% CYA drops were given in Group II. Only the solvent (alpha cyclodextrin), without any CYA, was applied to Group III, the control group, in the same manner. Suture removal and skin grafting were carried out on the 14th day after operation, as an additional means of sensitization. The 50-day post-operative rate of transparency retention was 40% for Group I, and 80% for Group II, and 20% for Group III, indicating that 0.05% CYA concentration was the most effective. In Experiment II, hetero-lamellar keratoplasties were performed on 16 albino rabbits to 8 Wistar rats, divided into Groups I and II. CYA (0.05% concentration) was applied 4 times a day for 50 days in Group I. Group II, the control group, was not treated. Both groups were followed for 100 days post operation. The 50-day post-operative rate of transparency retention of Group I was 71.4% and 12.5% for Group II. These figures also confirmed the effectiveness of 0.05% CYA. The results of our two experiments demonstrate that the application of 0.05% CYA would be most effective clinically.
Akiyama
S
S
Department of Ophthalmology, Juntendo University School of Medicine, Bunkyo-ku, Japan.
Yokoyama
T
T
Takano
T
T
Kobayashi
C
C
Kanai
A
A
D000818:Animals; D016039:Corneal Transplantation; D016572:Cyclosporine / Q000008:administration & dosage* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007166:Immunosuppressive Agents; D009883:Ophthalmic Solutions; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D016038:Skin Transplantation
D007166:Immunosuppressive Agents; D009883:Ophthalmic Solutions; D016572:Cyclosporine