title: Direct fluorescent-antibody confirmation of chlamydial antigen below the detection threshold of the chlamydiazyme enzyme-linked immunosorbent assay.
abstract: Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed with the direct fluorescent-antibody test (Syva) from among those 66 Chlamydiazyme-negative specimens which had A492s that ranged from 0.030 to the cutoff and that could be blocked by > or = 50% with the blocking reagent.
authors.affiliation: Immunology Laboratory, York Hospital, Pennsylvania 17405.
authors.forename: J A
authors.initials: JA
authors.lastname: Kellogg
authors.forename: J W
authors.initials: JW
authors.lastname: Seiple
authors.forename: E S
authors.initials: ES
authors.lastname: Stroll
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000276:immunology* / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005260:Female; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data; D006801:Humans; D011247:Pregnancy; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial