Amelioration of secondary ischaemic injury by perfusion with University of Wisconsin (UW) solution in rat skin flaps.
This study was designed to observe the effect of perfusion with University of Wisconsin (UW) preservation solution on skin flap survival following secondary ischaemia caused by venous obstruction in rats. An epigastric flap model was used. Saline-perfused flaps exhibited no significant improvement in survival compared to untreated animals (NS). Skin flaps perfused with UW solution, however, had a significant increase in survival to 40% (8/20) (p < 0.01) when perfused before the onset of primary ischaemia and 30% (p < 0.05) when given before the onset of secondary ischaemia. These results show that UW solution improves skin flap survival, presumably through preservation of the microvasculature.
Angel
M F
MF
Division of Plastic Surgery, Johns Hopkins University School of Medicine, Baltimore.
Knight
K R
KR
Becker
D
D
Amiss
L R
LR
Morgan
R F
RF
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D005978:Glutathione; D006085:Graft Survival / Q000187:drug effects; D007328:Insulin; D007511:Ischemia / Q000517:prevention & control*; D008297:Male; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply*; D016038:Skin Transplantation / Q000502:physiology*; D012996:Solutions; D013524:Surgical Flaps / Q000502:physiology*; D013997:Time Factors; D014021:Tissue Preservation
D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose