[Ultrastructural in situ hybridization on ultrathin sections of frozen tissues].
In situ hybridization on ultrathin frozen sections can be performed with either complementary DNA or synthetic oligonucleotide probes, labeled with 35S or an antigen (biotin or digoxigenin) respectively revealed by ultrastructural autoradiographic and immunocytological techniques. Using this method, GH mRNA was found in somatotrophs, mainly in the cytoplasm (endoplasmic reticulum and cytoplasmic matrix). Ultrastructural deterioration is the main drawback of this method but is more than outweighed by high sensitivity.
Pechoux
C
C
CNRS, URA 1454, Laboratoire d'Histologie-Embryologie, Faculté de Médecine Lyon-Sud, Oullins, France.
Jambou
R
R
Morel
G
G
D001345:Autoradiography; D015925:Cryopreservation; D013006:Growth Hormone / Q000235:genetics*; D017403:In Situ Hybridization / Q000379:methods*; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior; D012333:RNA, Messenger / Q000032:analysis* / Q000648:ultrastructure; D013696:Temperature
D012333:RNA, Messenger; D013006:Growth Hormone
Hybridation in situ ultrastructurale sur couples ultrafines de tissus congelés.