Subdiabetogenic streptozocin treatment impairs preimplantation development of mouse embryos.
To estimate the significance of insulin in the regulation of preimplantation embryo growth, female mice received a single subdiabetogenic dose of streptozocin (65 mg/kg intraperitoneally) 8-11 days or 14-17 days before fertilization. Mean glycaemia levels and the number of embryos per mouse did not differ significantly between the streptozocin-treated and control groups. Morphological analysis of preimplantation embryos collected on day 3 of pregnancy revealed significant changes in the distribution pattern of preimplantation embryo stages recovered from streptozocin-treated females. Continuous insulin treatment of streptozocin-treated mice improved the impaired development of preimplantation embryos only in short-lasting experiments. After a long subdiabetic period (14-17 days) the incidence of degenerated embryos was increased in both streptozocin-treated groups. It can be concluded that the subdiabetic state in female mice impairs preimplantation embryo development which could partly be prevented by insulin treatment.
Veselá
J
J
Institute of Animal Physiology, Slovak Academy of Sciences, Koŝice.
Rehák
P
P
Baran
V
V
Koppel
J
J
D000818:Animals; D047108:Embryonic Development / Q000187:drug effects*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D007328:Insulin / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D013311:Streptozocin / Q000008:administration & dosage / Q000494:pharmacology*
D007328:Insulin; D013311:Streptozocin