Cyclosporin A attenuates increased prostaglandin and thromboxane production in response to various stimuli in human decidua.
OBJECTIVE
This study was undertaken to evaluate the effects of cyclosporin A on prostanoid (prostaglandin and thromboxane) production by human decidua.


METHODS
Decidual cells were isolated from term placentae obtained at elective cesarean section before the onset of labor. Cells were grown to confluence and then incubated for 16 h with cyclosporin A (0.1-100 ng/ml) in the presence and absence of interleukin 1 beta (IL-1 beta, 10 ng/ml), phorbol 12-myristate 13-acetate (PMA, 10(-7) M) and ionomycin (0.5 micron). Prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) were measured by radioimmunoassay, and cellular protein was determined.


RESULTS
IL-1 beta, PMA, and ionomycin all stimulated decidual PGE2 and TXB2 production as expected. However, these stimulatory actions were attenuated by 20% when cells were coincubated with cyclosporin A (100 ng/ml). All concentrations of cyclosporin A tested were within the therapeutic range.


CONCLUSIONS
Our results indicate that cyclosporin A does not stimulate decidual prostanoid production and is probably unrelated to preterm labor and delivery in allograft recipients.
Edwin
S S
SS
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
Branch
D W
DW
Scott
J R
JR
Silver
R M
RM
Mitchell
M D
MD
D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D003656:Decidua / Q000187:drug effects* / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000502:physiology; D015759:Ionomycin / Q000037:antagonists & inhibitors; D011247:Pregnancy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013929:Thromboxane B2 / Q000096:biosynthesis*
D007375:Interleukin-1; D013929:Thromboxane B2; D015759:Ionomycin; D016572:Cyclosporine; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate