title: Expression of wnt10a in the central nervous system of developing zebrafish.
abstract: A polymerase chain reaction-derived clone with sequence homology to partial-length shark and salamander Wnt-10a was used to screen a zebrafish cDNA library, yielding a cDNA encoding a full-length Wnt polypeptide. Transcripts encoding zebrafish wnt10a are first detected during the segmentation period of embryogenesis, at a time when there is extensive development and differentiation of the central nervous system. wnt10a transcripts are confined to differentiating brain regions, with expression first appearing diffusely in the floor of the hindbrain ventricle and proceeding anteriorly to the mid- and forebrain. Comparatively, zebrafish wnt1 transcripts are expressed in a stripe at the future midbrain-hindbrain boundary and at the future forebrain-midbrain border. With the differentiation of the neural tube into distinct regions, wnt1 signals are also detected along the dorsal midline of the midbrain, the mid- and posterior hindbrain, and the spinal cord. These patterns suggest roles for wnt10a in the regionalization of the brain.
authors.affiliation: Department of Pharmacology SJ-30, University of Washington School of Medicine, Seattle 98195.
authors.forename: G M
authors.initials: GM
authors.lastname: Kelly
authors.forename: C J
authors.initials: CJ
authors.lastname: Lai
authors.forename: R T
authors.initials: RT
authors.lastname: Moon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry; D017124:Conserved Sequence; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D051153:Wnt Proteins; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins
chemical_list: D009419:Nerve Tissue Proteins; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; C082156:Wnt10a protein, zebrafish; D029961:Zebrafish Proteins