Analysis of the c-myc and N-ras genes in acute myelogenous leukemia cells which manifest the constitutive expression of the c-myc gene.
The "constitutive" expression of the c-myc gene was detected in leukemia cells obtained from 4 patients with an acute myeloid leukemia, 3 with chronic myeloid leukemia and 1 with a myelodysplastic syndrome. Studies were undertaken to determine whether or not the myc gene was rearranged or mutated in exon I within a 3'Pvu II region, a transcriptional attenuation site. Studies to explore the possibility of a point mutation in the N-ras gene were also conducted. No abnormalities were detected in either gene. Studies should be undertaken evaluating the possibility of a post-transcriptional mechanism, such as alteration of RNA stability, which could be responsible for the constitutive expression of c-myc gene.
Kadam
P
P
University of Cincinnati Medical Center, Ohio.
Steele
P
P
Li
Y Q
YQ
Preisler
H
H
D000208:Acute Disease; D001483:Base Sequence; D015139:Blotting, Southern; D015321:Gene Rearrangement; D016259:Genes, myc / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D016274:Oncogene Protein p55(v-myc) / Q000032:analysis; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis
D016274:Oncogene Protein p55(v-myc); D012334:RNA, Neoplasm; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)