[Effects of growth factors on the gene expression of extracellular matrix components in cultured rat mesangial cells].
The accumulation of mesangial matrix is a common histologic abnormality observed over the course of various glomerular disease. To determine the role of growth factors in the mesangial matrix metabolism, the effects of the transforming growth factor-beta (TGF-beta), interleukin-1 beta (IL-1 beta), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), endothelin (ET), and insulin-like growth factor (IGF) on the gene expression of mesangial matrix were studied in cultured rat mesangial cells (MCs). TGF-beta induced transcriptional activation of the genes for alpha 1 (I) and alpha 1 (IV) collagens and fibronectin, and the mRNA levels for these genes in MCs treated with TGF-beta for 24 hours resulted in 1.9-, 2.8-, 2.6-fold increase, respectively, compared to their basal levels. IL-1 beta also transcriptionally increased the mRNA levels for alpha 1 (I) alpha 1 (IV) collagens and fibronectin in the non-stimulated MCs, however, inhibited the increase of these mRNA levels in the TGF-beta-treated MCs. MCs showed little increase in the gene expression for alpha 1 (IV) collagen and fibronectin by the addition of PDGF, EGF, and ET, and no change was noted in the IGF-treated MCs. Detectable gene expression for B1 chain of laminin and heparan-sulphate proteoglycan was observed in the non-stimulated MCs but small in intensity, and no change was noted by stimulation of any growth factors examined.(ABSTRACT TRUNCATED AT 250 WORDS)
Suzuki
S
S
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
D000818:Animals; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005920:Glomerular Mesangium / Q000166:cytology*; D006133:Growth Substances / Q000494:pharmacology* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic / Q000187:drug effects
D016326:Extracellular Matrix Proteins; D006133:Growth Substances; D012333:RNA, Messenger