Differentiation of mumps vaccine strains from wild viruses by single-strand conformation polymorphism of the P gene.
The DNA fragments amplified through the polymerase chain reaction from part of the P gene of four mumps vaccine strains (Urabe, Torii, Hoshino and Miyahara) were subjected to single-strand conformation polymorphism (SSCP) analysis. These four vaccine strains were differentiated from each other. Furthermore, twelve wild viruses and a laboratory strain (Enders strain) were also distinguished by this method. Viruses isolated from patients who developed aseptic meningitis 4 to 6 weeks after measles-mumps-rubella vaccination showed identical SSCP patterns with the vaccine strain used for immunization. These results were well correlated with sequence analysis of P-gene segments, indicating high applicability of the SSCP analysis for differentiation of mumps vaccine strains not only from each other but from wild viruses.
Katayama
K
K
Basic Research Division, Bio Medical Laboratory Inc., Saitama, Japan.
Oya
A
A
Tanabayashi
K
K
Okazaki
K
K
Hishiyama
M
M
Yamazaki
S
S
Yamada
A
A
D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D004338:Drug Combinations; D005814:Genes, Viral; D006801:Humans; D008458:Measles Vaccine / Q000009:adverse effects; D022542:Measles-Mumps-Rubella Vaccine; D008582:Meningitis, Aseptic / Q000209:etiology; D009108:Mumps Vaccine / Q000009:adverse effects; D009109:Mumps virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D009690:Nucleic Acid Conformation; D010309:Parotitis / Q000209:etiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012411:Rubella Vaccine / Q000009:adverse effects; D014611:Vaccination
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004338:Drug Combinations; D008458:Measles Vaccine; D022542:Measles-Mumps-Rubella Vaccine; D009108:Mumps Vaccine; D012411:Rubella Vaccine