[Production of recombinant proteins and their use in immunoblotting].
Chemico-enzymatic synthesis and cloning of genes coding viral proteins--precursors of p50 and gp120 in the eukaryotic vector system were carried out. The recombinant proteins obtained were shown to be used in immunochemical evaluation of AIDS virus antibodies in blood serum.
Farkhutdinov
M R
MR
Galiullin
F G
FG
Davletov
E G
EG
Gabbasov
Sh F
ShF
Safin
B N
BN
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D015153:Blotting, Western / Q000379:methods*; D003001:Cloning, Molecular; D015683:Gene Products, gag / Q000235:genetics; D015483:HIV Antibodies / Q000032:analysis; D015488:HIV Antigens / Q000235:genetics; D016655:HIV Core Protein p24 / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D006801:Humans; D011498:Protein Precursors / Q000235:genetics; D011994:Recombinant Proteins / Q000138:chemical synthesis; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D011498:Protein Precursors; D011994:Recombinant Proteins; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1
Poluchenie rekombinantnykh belkov i ikh ispol'zovanie v metode immunoblotta.