title: Sequential reactivity of serum against cyst antigens in Toxoplasma infection.
abstract: OBJECTIVE
  To compare the recognition of Toxoplasma gondii tachyzoites and cysts by sera, from 10 patients.
  
  
  METHODS
  Recognition of antigens from purified tachyzoites (RH strain) and bradyzoites (18691 strain) was compared using western immunoblotting. Sequential serum samples from 10 patients and one laboratory acquired RH infection were used.
  
  
  RESULTS
  Recognition of cyst antigens was relatively low and occurred late in infection. The two stages were antigenically distinct with only a few shared bands.
  
  
  CONCLUSIONS
  Immunological recognition of the cystic stage of T gondii is low. This implies that either cysts are poorly immunogenic or that cyst antigen is not available for processing and presentation.
authors.affiliation: Department of Pure and Applied Biology, University of Leeds.
authors.forename: G
authors.initials: G
authors.lastname: Woodison
authors.forename: A H
authors.initials: AH
authors.lastname: Balfour
authors.forename: J E
authors.initials: JE
authors.lastname: Smith
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D015153:Blotting, Western; D006801:Humans; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan