title: Role of nitric oxide in the vasodilator but not exudative component of mustard oil-induced inflammation in rat skin.
abstract: The participation of nitric oxide (NO) in the neurogenic inflammatory reaction of the rat hindpaw skin to topical application of mustard oil was examined by the use of NG-nitro-L-arginine methyl ester (L-NAME, 43 mumol kg-1 i.v.), an inhibitor of NO formation. Control rats received the same dose of the inactive enantiomer D-NAME. Vasodilatation was recorded by contactless infrared emission thermography, and plasma protein exudation was measured by the Evans Blue leakage technique and by measurement of the paw volume in anaesthetized rats. L-NAME reduced the cutaneous hyperaemia caused by topical administration of mustard oil by about 50% but did not change the exudative reaction to mustard oil. These findings indicate that NO plays a mediator role in the vasodilator component of neurogenic inflammation in the rat paw skin, whereas the increase in vascular permeability does not appear to depend on NO.
authors.affiliation: University of Graz, Department of Pharmacology, Austria.
authors.forename: I T
authors.initials: IT
authors.lastname: Lippe
authors.forename: A
authors.initials: A
authors.lastname: Stabentheiner
authors.forename: P
authors.initials: P
authors.lastname: Holzer
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D003877:Dermatitis, Contact / Q000378:metabolism / Q000503:physiopathology*; D004487:Edema / Q000139:chemically induced; D005122:Exudates and Transudates / Q000187:drug effects / Q000502:physiology*; D005260:Female; D009149:Mustard Plant; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D010936:Plant Extracts; D010938:Plant Oils; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012881:Skin Temperature / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D010938:Plant Oils; D009569:Nitric Oxide; D001120:Arginine; C027793:mustard oil; D019331:NG-Nitroarginine Methyl Ester