A simple method for the purification of Eimeria tenella sporozoites.
A rapid, simple and cheap method for the purification of Eimeria tenella sporozoites has been developed using commercially available filter paper (595 filter paper circles, Schleicher & Schuell, 3354 Dassel, Germany, order 311610). Yield and purity are of the same value as by purification with a leukopac column (Bontemps & Yvore 1974). The described method can be used for the purification of Eimeria tenella sporozoites under sterile conditions for subsequent in vitro cultivation.
Mattig
F R
FR
Institut of Zoology, University of Bonn, Germany.
Drössigk
U
U
Entzeroth
R
R
D000818:Animals; D016786:Eimeria tenella / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D005374:Filtration / Q000379:methods