Transforming growth factor-beta in rat kidney during compensatory renal growth.
Transforming growth factor-beta (TGF-beta) inhibits the DNA synthesis and stimulates the alkaline phosphatase activity of cultured renal cortical tubular cells. When unilateral nephrectomy was performed, TGF-beta activity content gradually decreased in the remaining kidney. An immunohistochemical study of the remaining kidney after uninephrectomy, however, showed that the expression of TGF-beta increased by the third day after uninephrectomy in the proximal tubular cells and an autoradiographic study revealed that TGF-beta expressing tubular cells were different from proliferating cells during compensatory renal growth. These results indicate that the total renal TGF-beta content decreases after uninephrectomy, while the expression of TGF-beta in the proximal tubular cells may increase and act as a brake for compensatory renal hyperplasia.
Kanda
S
S
Department of Urology, Nagasaki University School of Medicine, Japan.
Igawa
T
T
Taide
M
M
Eguchi
J
J
Nakamura
M
M
Nomata
K
K
Yamada
J
J
Kanetake
H
H
Saito
Y
Y
D000222:Adaptation, Physiological; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007150:Immunohistochemistry; D007668:Kidney / Q000254:growth & development* / Q000378:metabolism*; D008297:Male; D009392:Nephrectomy; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D016212:Transforming Growth Factor beta