An enzymological profile of the production of lactic acid in caries-associated plaque and in plaque formed on sound surfaces of deciduous teeth.
Specific activities of lactate dehydrogenase (LDH) and its fructose 1,6-bisphosphate (FBP) dependency in the small amount of plaque formed on deciduous teeth were determined and compared between those from carious lesions and from sound surfaces. Although the caries-associated plaque showed a higher production of lactic acid than the plaque formed on sound surfaces, it occurred only when sufficient FBP was present. The proportion of activity of FBP-dependent LDH relative to that of total LDH varied much more in the caries-associated plaque than in the plaque formed on sound surfaces. Hence, the varying activity of FBP-dependent LDH might be a distinguishing feature of caries-associated plaque compared with plaque formed on sound surfaces.
Tanaka
H
H
Department of Bacteriology, Nihon University School of Dentistry, Tokyo, Japan.
Tamura
M
M
Kikuchi
K
K
Kuwata
F
F
Hirano
Y
Y
Hayashi
K
K
D002648:Child; D002675:Child, Preschool; D003731:Dental Caries / Q000201:enzymology*; D003743:Dental Enamel / Q000378:metabolism; D003773:Dental Plaque / Q000737:chemistry / Q000201:enzymology*; D005260:Female; D005635:Fructosediphosphates / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D007773:Lactates / Q000096:biosynthesis*; D019344:Lactic Acid; D008297:Male; D011506:Proteins / Q000032:analysis; D014094:Tooth, Deciduous / Q000378:metabolism*
D005635:Fructosediphosphates; D007773:Lactates; D011506:Proteins; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; C029063:fructose-1,6-diphosphate