Development of monoclonal antibodies for the detection of Mycoplasma pneumoniae.
Mycoplasma pneumoniae-specific monoclonal antibodies were constructed for the purpose of developing reagents for clinical diagnostics. The monoclonal antibodies, designated Mp2B12 and Mp3A11, recognize surface exposed antigens of 28 kDa and 170 kDa, respectively. Mp3A11 recognizes an epitope on a cytadhesin molecule, P1, which is not shared on the analogous cytadhesin of M. genitalium. Both monoclonal antibodies are capable of detecting 1 x 10(5) M. pneumoniae in a standard ELISA test.
Geary
S J
SJ
Department of Pathobiology, University of Connecticut, Storrs 06269-3089.
Ryan
J A
JA
Forsyth
M H
MH
Sasseville
V
V
D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D001419:Bacteria / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008970:Molecular Weight; D009174:Mycoplasma / Q000145:classification / Q000276:immunology; D009177:Mycoplasma pneumoniae / Q000276:immunology* / Q000302:isolation & purification; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins