Targeting of microdiscs in white cells to tibial abscesses in rabbits.
The ability to deliver drugs to specific foci of infection is a sought-after goal. One solution is to use microparticles as drug carriers. This approach is limited by detection of microparticles by the reticuloendothelial system (RES). In order to reduce RES uptake of such particles, we investigated the possibility of "hiding" microparticles within white cells prior to targeting them to experimental tibial abscesses. We used radioactive silicone microdiscs, supplied by the Royal Signals & Radar Establishment. Twelve rabbits with abscesses in the right tibia were used: six control animals received radioactive opsonised microdiscs intravenously, and six animals received the same dose of microdiscs following incubation of the microdiscs with white cells. Each animal's liver, spleen, lungs, and both tibiae were removed, weighed, and homogenised. Radioactivity counts were obtained from each tissue, and the ratio of counts per gram of tissue for the right/left tibiae was calculated for the two groups of animals. The ratio of counts in the control group was 1.66 (+/- 0.57 SD), and the mean ratio of counts from the rabbits who had microdisc incubated with white cells was 3.32 (+/- 0.52 SD). This difference was statistically significant at p = 0.02 (Mann-Whitney U test).
Bhamra
M
M
Academic Surgical Unit, Queen Elizabeth the Queen Mother Wing, St Mary's Hospital, London, England.
Tyrrell
M R
MR
Hulme
A L
AL
Dye
J F
JF
Dudley
H A
HA
D000038:Abscess / Q000188:drug therapy*; D000818:Animals; D001847:Bone Diseases / Q000188:drug therapy; D004337:Drug Carriers; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000627:therapeutic use; D007275:Injections, Intravenous; D007962:Leukocytes / Q000502:physiology*; D008863:Microspheres; D009504:Neutrophils / Q000502:physiology; D010316:Particle Size; D010587:Phagocytosis; D011817:Rabbits; D012828:Silicones; D013977:Tibia; D014343:Trypan Blue
D004337:Drug Carriers; D007074:Immunoglobulin G; D012828:Silicones; D014343:Trypan Blue