Isolation and characterization of two new functional subtypes of HLA-B35.
Human minor histocompatibility antigen-specific, HLA-B*3501-restricted cytotoxic T-cell clones were assayed on a panel of 25 different target cells previously typed by serology as HLA-B35. Cells from 6 donors were not killed and cells from 2 of these were further studied by molecular cloning to characterize their HLA class I alleles. Two new HLA-B35 subtypes were identified. The sequence of one of them differs from B*3501 by one nucleotide change at codon 156, replacing a Leu for an Arg. The sequence of the other new subtype also shows a single nucleotide change compared to B*3501, with Gly-->Val substitution at residue 16. With these new variants, the allelic complexity of HLA-B35 extends now to eight subtypes.
Theiler
G
G
Laboratorio de Inmunogenética, Hospital de Clínicas, Argentina.
Pando
M
M
Delfino
J M
JM
Takiguchi
M
M
Satz
M L
ML
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D016384:Consensus Sequence; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000235:genetics; D005805:Genes, MHC Class I; D015797:HLA-B35 Antigen / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D017385:Sequence Homology
D015797:HLA-B35 Antigen; D011994:Recombinant Proteins; D004247:DNA