[Effect of storage conditions on the infectivity of Chlamydia pneumonia].
In order to facilitate the isolation of C. pneumoniae, strain similar TWAR, with high frequency, we investigated the effect of various factors on the infectivity of Chlamydia using two laboratory strains, C. pneumoniae TWAR and C. trachomatis serovar D. The factors tested were the effects of different temperatures for storage conditions, saliva from healthy person, storage media for Chlamydia, and the frequency of freezing and thawing. Chlamydial suspension was prepared in the two media, SPG (sucrose-phosphate-glutamate buffer pH 7.5), and CT-GM (culture medium for Chlamydia which contains 1 micrograms/ml cycloheximide and 0.04% glucose). Chlamydial suspension was allowed to stand in each of four different thermal conditions: 37 degrees C, room temperature (25 degrees C), 4 degrees C, 0 degrees C and -75 degrees C for 1, 3, 6, 24, 48, 72, 96, 120 and 144 hours. For storage at -75 degrees C, one of three groups of glass vial tubes containing Chlamydia was covered with an "airmat" to prevent the rapid freezing of Chlamydia. The effect of various factors on the infectivity was assayed by inoculation of the suspension on HeLa 229 cell monolayers. Results showed that the infectivity rapidly decreased at 37 degrees C and room temperature, while at 4 degrees C, 0 degrees C and -75 degrees C, relatively high infectivity was maintained and contained until days 4 to 6. This decreasing pattern was similar to among the media used. We were not able to find any differences in the infectivity among the samples with or without the "airmat".(ABSTRACT TRUNCATED AT 250 WORDS)
Komoda
T
T
Department of Public Health, Kyorin University School of Health Sciences, Japan.
Bannai
H
H
Miyazawa
H
H
Tezuka
T
T
Hagiwara
T
T
Shiga
S
S
Ashihara
Y
Y
D002689:Chlamydia / Q000472:pathogenicity*; D002692:Chlamydia trachomatis / Q000472:pathogenicity; D003470:Culture Media; D005615:Freezing; D006801:Humans; D012463:Saliva / Q000502:physiology; D013048:Specimen Handling; D013696:Temperature
D003470:Culture Media