Methylation patterns in the human muscle-specific enolase gene (ENO3).
The methylation status in the human-muscle enolase gene (ENO3) was assayed. Previous sequence data and MspI cleavage sites indicate the presence of a 5' CpG-rich island of at least 4 kb: none of 22 characterized MspI CCGG sites is methylated in any of muscle, sperm or brain DNA. However a complex pattern of complete and partial methylation of MspI sites that is different between tissues is observed within the ENO3 gene: events at one site may be specific to muscle DNA. The absence of methylation in the promoter region of the ENO3 gene makes it unlikely that methylation plays a causal role either in transcriptional events or in the divergence of enolase-isogene regulation. The role of tissue-specific methylation events within ENO3 remains to be determined.
Peshavaria
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University Department of Clinical Biochemistry, Southampton General Hospital, U.K.
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D000328:Adult; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005091:Exons; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D008745:Methylation; D009132:Muscles / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D015183:Restriction Mapping; D012661:Semen / Q000201:enzymology; D016385:TATA Box
D004247:DNA; D010751:Phosphopyruvate Hydratase