title: Biosynthesis of riboflavin: cloning, sequencing, mapping, and expression of the gene coding for GTP cyclohydrolase II in Escherichia coli.
abstract: GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated an open reading frame of 588 bp coding for a 21.8-kDa peptide of 196 amino acids. The gene was mapped to a position at 28.2 min on the E. coli chromosome and is identical with ribA. GTP cyclohydrolase II was overexpressed in a recombinant strain carrying a plasmid with the cloned gene. The enzyme was purified to homogeneity from the recombinant strain. The N-terminal sequence determined by Edman degradation was identical to the predicted sequence. The sequence is homologous to the 3' part of the central open reading frame in the riboflavin operon of Bacillus subtilis.
authors.affiliation: Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Garching, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Richter
authors.forename: H
authors.initials: H
authors.lastname: Ritz
authors.forename: G
authors.initials: G
authors.lastname: Katzenmeier
authors.forename: R
authors.initials: R
authors.lastname: Volk
authors.forename: A
authors.initials: A
authors.lastname: Kohnle
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: D
authors.initials: D
authors.lastname: Allendorf
authors.forename: A
authors.initials: A
authors.lastname: Bacher
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D006136:GTP Cyclohydrolase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012256:Riboflavin / Q000096:biosynthesis*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D011994:Recombinant Proteins; D006136:GTP Cyclohydrolase; D012256:Riboflavin