title: NAH plasmid-mediated catabolism of anthracene and phenanthrene to naphthoic acids.
abstract: Pseudomonas fluorescens 5R contains an NAH7-like plasmid (pKA1), and P. fluorescens 5R mutant 5RL contains a bioluminescent reporter plasmid (pUTK21) which was constructed by transposon mutagenesis. Polymerase chain reaction mapping confirmed the localization of lux transposon Tn4431 300 bp downstream from the start of the nahG gene. Two degradation products, 2-hydroxy-3-naphthoic acid and 1-hydroxy-2-naphthoic acid, were recovered and identified from P. fluorescens 5RL as biochemical metabolites from the biotransformation of anthracene and phenanthrene, respectively. This is the first report which provides direct biochemical evidence that the naphthalene plasmid degradative enzyme system is involved in the degradation of higher-molecular-weight polycyclic aromatic hydrocarbons other than naphthalene.
authors.affiliation: Center for Environmental Biotechnology, University of Tennessee, Knoxville 37932.
authors.forename: F M
authors.initials: FM
authors.lastname: Menn
authors.forename: B M
authors.initials: BM
authors.lastname: Applegate
authors.forename: G S
authors.initials: GS
authors.lastname: Sayler
mesh_terms: D000873:Anthracenes / Q000378:metabolism*; D001431:Bacteriological Techniques; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002874:Chromosome Mapping; D003470:Culture Media / Q000737:chemistry; D008969:Molecular Sequence Data; D009284:Naphthols / Q000737:chemistry* / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D003470:Culture Media; D009284:Naphthols; D010616:Phenanthrenes; C031181:phenanthrene; C034020:anthracene