Preliminary screening of non-platinum complexes of Schiff bases as antitumour agents using fluorimetry.
Based on the consistency of the in vivo and in vitro interactions of drugs with DNA, a fluorimetric method has been developed as a new in vitro method for preliminary screening of antitumour agents. This method was tested using Schiff bases synthesized from salicylaldehyde with 1-alanine, 1-asparagine and 1-histidine, and complexes of these Schiff bases with Cu(II), Zn(II), Ni(II) and Sn(IV) as potential antitumour agents. The study of the interaction of the complexes with DNA by a fluorescence probe ethidium bromide (EthBr)-DNA system indicated the parallelism between the binding constants and antineoplastic ratios. The relationship between structure and antitumour activity was investigated.
Li
Z L
ZL
Department of Chemistry and Chemical Engineering, Hunan University, Changsha, PRC.
Chen
J H
JH
Zhang
K C
KC
Li
M L
ML
Yu
R Q
RQ
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003300:Copper / Q000494:pharmacology / Q000627:therapeutic use; D004247:DNA / Q000378:metabolism; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009532:Nickel / Q000494:pharmacology / Q000627:therapeutic use; D012510:Sarcoma 180 / Q000188:drug therapy; D012545:Schiff Bases; D013050:Spectrometry, Fluorescence
D000970:Antineoplastic Agents; D012545:Schiff Bases; D003300:Copper; D009532:Nickel; D004247:DNA