title: Compounds biologically similar to platelet activating factor are present in stored blood components.
abstract: Agents which prime the neutrophil NADPH oxidase develop during routine storage of whole blood and packed red blood cells. This plasma priming activity can be inhibited by bepafant (WEB 2170), a specific platelet activating factor (PAF) receptor antagonist. Quantitation of the priming agent(s), by a commercially available radioimmunoassay for PAF, reproducibly demonstrated high levels of PAF activity. However, analysis of these plasma samples from stored blood components by gas chromatography/mass spectroscopy did not reveal any 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine. We conclude that the polyclonal antibody to PAF used in these studies may have recognized different epitopes of a family of heterogeneous, biologically active lipids that manifest their effects through the PAF receptor.
authors.affiliation: Department of Pediatrics, University of Colorado School of Medicine, Denver.
authors.forename: C C
authors.initials: CC
authors.lastname: Silliman
authors.forename: C A
authors.initials: CA
authors.lastname: Johnson
authors.forename: K L
authors.initials: KL
authors.lastname: Clay
authors.forename: G W
authors.initials: GW
authors.lastname: Thurman
authors.forename: D R
authors.initials: DR
authors.lastname: Ambruso
mesh_terms: D001381:Azepines / Q000494:pharmacology*; D001793:Blood Preservation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000097:blood; D019255:NADPH Oxidases; D009504:Neutrophils / Q000201:enzymology; D010972:Platelet Activating Factor / Q000032:analysis* / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011863:Radioimmunoassay; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D001381:Azepines; D010972:Platelet Activating Factor; D014230:Triazoles; C063964:bepafant; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases