Three exceptional IgH/myc-translocation-carrying rat immunocytomas have breakpoints 50 to 80 kb 5' of c-myc.
The spontaneously arising immunocytoma of the Louvain rat (RIC) carries a consistent chromosomal translocation between chromosomes 6 and 7. This translocation juxtaposes immunoglobulin heavy chain and c-myc sequences. In an earlier study on 14 RIC tumors, we found that the translocation breakpoint is located within 1.5 kb immediately upstream of c-myc in 10 of the tumors. Here we describe 3 exceptional tumors that had no rearrangement within 20 kb 5' of c-myc. Using pulsed-field gel electrophoresis we show that the translocation breakpoints in these tumors are located 50-80 kb 5' of c-myc and that c-myc rearranges to the 3' end of the IgH cluster.
Axelson
H
H
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Panda
C K
CK
Klein
G
G
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D004273:DNA, Neoplasm / Q000032:analysis; D005803:Genes, Immunoglobulin; D016259:Genes, myc; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000276:immunology; D005810:Multigene Family; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains