Ca(2+)-induced increase in oxidative metabolism of dissociated mammalian brain neurons: effect of extract of ginkgo biloba leaves.
Effect of an extract of Ginkgo biloba leaves (EGb) on oxidative metabolism was studied using rat brain neurons and 2',7'-dichlorofluorescin fluorescence. Ionomycin (100 nM to 1 microM), a Ca(2+)-ionophore, dose-dependently augmented the 2',7'-dichlorofluorescin fluorescence in the presence of external Ca2+, but not under the external Ca(2+)-free condition. Preincubation of neurons with EGb (3 micrograms/ml) greatly reduced the ionomycin-induced increase in 2',7'-dichlorofluorescin fluorescence. Results suggest that EGb may reduce the Ca(2+)-induced increase in the oxidative metabolism of brain neurons.
Oyama
Y
Y
Division of Pharmacological Science, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Hayashi
A
A
Ueha
T
T
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects; D005452:Fluoresceins; D066298:In Vitro Techniques; D015759:Ionomycin / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY
D005452:Fluoresceins; D010936:Plant Extracts; D015759:Ionomycin; C037631:2',7'-dichlorofluorescein; D002118:Calcium