title: Column-switching solid-phase trace-enrichment high-performance liquid chromatographic method for measurement of buprenorphine and norbuprenorphine in human plasma and urine by electrochemical detection.
abstract: We describe a new high-performance liquid chromatographic method using electrochemical detection for the determination of buprenorphine and norbuprenorphine in plasma and urine. The minimum concentration for detection of buprenorphine and norbuprenorphine is 40 pg/ml. The intra-assay coefficient of variation (C.V.) in plasma and urine samples ranges from 6 to 17% depending on the drug concentration. At a plasma concentration of 500 pg/ml the inter-assay C.V. is 8% for buprenorphine and 9% for norbuprenorphine. The analysis duration is 16 min. After solid-phase extraction and evaporation a valve-switching system with two Rheodyne valves enables sample enrichment, optimal sample cleaning and rapid elution of long-retained substances.
authors.affiliation: Department of Internal Medicine, University of Göttingen, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Schleyer
authors.forename: R
authors.initials: R
authors.lastname: Lohmann
authors.forename: C
authors.initials: C
authors.lastname: Rolf
authors.forename: A
authors.initials: A
authors.lastname: Gralow
authors.forename: C C
authors.initials: CC
authors.lastname: Kaufmann
authors.forename: M
authors.initials: M
authors.lastname: Unterhalt
authors.forename: W
authors.initials: W
authors.lastname: Hiddemann
mesh_terms: D002047:Buprenorphine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004355:Drug Stability; D004563:Electrochemistry; D006801:Humans
chemical_list: D002047:Buprenorphine; C043585:norbuprenorphine