Effects of calcitonin on CNS monoamines following carrageenan-induced inflammation in rats.
The present study examined the effects of systemically administered calcitonin (CT, 10 IU/0.25 ml, SC) on changes in CNS monoamines (MAs) following unilateral carrageenan (CARRA)-induced inflammation in the rat hindpaw. High-performance liquid chromatography with electrochemical detection (HPLC-EC) for MAs was performed on the whole brain and rostral spinal cord. Carrageenan-evoked inflammation significantly increased brain serotonin [5-hydroxytryptamine (5-HT)], norepinephrine (NE), and dopamine (DA) levels. CT significantly reduced these CARRA-induced elevations in brain MAs. Elevated spinal cord 5-HT and NE levels were observed in CARRA-treated animals. CT administration increased 5-HT and NE in both the CARRA-treated animals and their saline controls. Spinal cord DA levels were not affected by either CARRA or CT administration. These findings suggest the involvement of CNS monoaminergic substrates in CT-induced hypoalgesia in inflammatory nociception.
Sufka
K J
KJ
Department of Psychology, University of Mississippi, Oxford 38677.
Hoganson
D A
DA
D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002116:Calcitonin / Q000494:pharmacology*; D002351:Carrageenan; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D009619:Nociceptors / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism
D015306:Biogenic Monoamines; D002351:Carrageenan; D002116:Calcitonin