Pronounced enhancement of .NO-dependent antimicrobial action by an .NO-oxidizing agent, imidazolineoxyl N-oxide.
The antimicrobial action of .NO against Cryptococcus neoformans was investigated by using imidazolineoxyl N-oxide, which we recently reported removes .NO via oxidation (T. Akaike, M. Yoshida, Y. Miyamoto, K. Sato, M. Kohno, K. Sasamoto, K. Miyazaki, S. Ueda, and H. Maeda, Biochemistry 32:827-832, 1993). No appreciable fungicidal activity was observed in neutral .NO solutions. Imidazolineoxyl N-oxide induced or enhanced fungicidal action in neutral or acidic .NO solutions, respectively. Our results provide convincing evidence that .NO is not a microbicidal molecular species.
Yoshida
K
K
Department of Microbiology, Kumamoto University School of Medicine, Japan.
Akaike
T
T
Doi
T
T
Sato
K
K
Ijiri
S
S
Suga
M
M
Ando
M
M
Maeda
H
H
D000890:Anti-Infective Agents / Q000494:pharmacology*; D003455:Cryptococcus neoformans / Q000187:drug effects; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*
D000890:Anti-Infective Agents; D003497:Cyclic N-Oxides; D007093:Imidazoles; C079391:2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; D009569:Nitric Oxide