[Evaluation of DNA-HIV detection in various population groups].
BACKGROUND
To evaluate the diagnostic use of Polymerase Chain Reaction (PCR) for the detection of HIV-DNA.


METHODS
We have investigated HIV-DNA in peripheral blood mononuclear cells of 150 individuals: 50 seronegative without recognized factors to acquire HIV infection, 50 seronegative that showed risk habits and 50 seropositive. For amplification we have used primers SK38/SK39 which amplify a conserved region of 114 bp from HIV's gag gen. The detection of the amplificate was performed using solution hybridization with 32P-labelled SK 19, 6% polyacrylamide gel electrophoresis and autoradiography.


RESULTS
We have detected HIV-DNA at every seropositive patient (100%), 6 samples from seronegative individuals that showed risk habits and 1 sample from non-risk seronegative individuals.


CONCLUSIONS
Polymerase chain reaction represents a diagnostic tool at HIV infection, specially for those patients where seroconversion can not be demonstrated by conventional methods.
Quirós
E
E
Departamento de Microbiología (Facultad de Medicina), Universidad de Granada.
García
F
F
Cabezas
T
T
González
I
I
Bernal
M C
MC
Maroto
M C
MC
Piedrola
G
G
D004279:DNA, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012307:Risk Factors
D004279:DNA, Viral
Evaluación de la detección de ADN-VIH en diversos grupos de población.