Demonstration of lectin-like activity for the common antigen of Neisseria gonorrhoeae by quantitative immunoelectrophoretic techniques.
To overcome problems associated with Western blotting of denatured proteins, we have used quantitative immunoelectrophoretic techniques to perform functional analysis of the Neisseria gonorrhoeae common antigen. Using these techniques, we show (a) that Neisseria gonorrhoeae expresses an antigen that is cross-reactive with the common antigen of Pseudomonas aeruginosa and Legionella micdadei and with the GroEl-like protein of Chlamydia, and (b) that this N. gonorrhoeae common antigen has lectin-like activity and can be precipitated with three different sugars immobilized on agarose beads: alpha-D-glucosamine, maltose and fucose.
Benkirane
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R
Institut Pasteur de Lyon, Département de Bactériologie-Virologie, France.
Denoyel
G A
GA
de Montclos
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H
D000942:Antigens, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D050260:Carbohydrate Metabolism; D003429:Cross Reactions; D007123:Immunoelectrophoresis, Two-Dimensional; D037102:Lectins / Q000302:isolation & purification*; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000276:immunology* / Q000378:metabolism
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D037102:Lectins