Production and characterization of antibodies reactive with leukotoxin.
A sensitive radioimmunoassay was developed for the determination of leukotoxin (9: 10-epoxy-12-octadecenoic acid), which was reported to exist in human burned skin and neutrophils, and was regarded as a toxic and/or defensive substance in living beings. The leukotoxin was conjugated with bovine serum albumin by means of the mixed anhydride technique as immunogen and rabbits were immunized over 4 months. According to a titration test of antiserum, forty-fold diluted antiserum was found to bind approximately 50% of about 4,000 dpm of methylated leukotoxin labeled with [14C] carbon. The detection limit of leukotoxin was at least as low as 5 ng in this radioimmunoassay by use of a polyetylene glycol precipitation method. This antiserum had a strong specificity to leukotoxin and no cross-reactivity with the other analogs tested.
Akabane
H
H
Obihiro Kyokai Hospital, Japan.
Nagao
M
M
Zhang
W D
WD
Yamada
Y
Y
Oono
T
T
Takatori
T
T
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D008041:Linoleic Acids / Q000276:immunology*; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods
D000906:Antibodies; D008041:Linoleic Acids; C051208:9,10-epoxy-12-octadecenoic acid