Phospholipase D treatment enhances gonadotrophin receptor-coupled adenylate cyclase activity in isolated bovine luteal cells.
LH-stimulated adenylate cyclase activity in membrane preparations of bovine luteal cells could be enhanced by treating the cells with either phospholipase D or its hydrolysis product, phosphatidic acid. Similar augmentary effects were also produced following treatment of the cells with EGF. Moreover, EGF could stimulate the formation of [3H]phosphatidic acid in [3H]myristic acid preloaded cells, suggesting that EGF is able to activate cellular phospholipase D. Also, PMA was able to increase the phosphatidic acid formation with a parallel increase in the adenylate cyclase activity. We propose, therefore, that phosphatidic acid may act as an intracellular second messenger linking EGF-mediated activation of phospholipase D with the sensitization of LH receptor-coupled adenylate cyclase signalling system.
Budnik
L T
LT
Institute for Hormone and Fertility Research, University of Hamburg, Germany.
Mukhopadhyay
A K
AK
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D005576:Colforsin / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000502:physiology; D005260:Female; D006160:Guanosine Triphosphate / Q000494:pharmacology; D008184:Luteal Cells / Q000187:drug effects / Q000201:enzymology*; D007986:Luteinizing Hormone / Q000494:pharmacology; D010712:Phosphatidic Acids / Q000378:metabolism / Q000494:pharmacology; D010739:Phospholipase D / Q000494:pharmacology*; D011967:Receptors, Gonadotropin / Q000502:physiology*
D010712:Phosphatidic Acids; D011967:Receptors, Gonadotropin; D005576:Colforsin; D004815:Epidermal Growth Factor; D006160:Guanosine Triphosphate; D007986:Luteinizing Hormone; D066246:ErbB Receptors; D010739:Phospholipase D; D000262:Adenylyl Cyclases