The isolation of cDNAs from OATL1 at Xp 11.2 using a 480-kb YAC.
Using an ornithine-delta-aminotransferase (OAT) cDNA, we identified five YACs that cover two nonadjacent OAT-related loci in Xp11.2-p11.3, designated OATL1 (distal) and OATL2 (proximal). Because several retinal degenerative disorders map to this region, we used YAC2 (480 kb), which covers the most distal part of OATL1, as a probe to screen a retinal cDNA library. From 8 x 10(4) plaques screened, we isolated 13 clones. Two were OAT cDNAs. The remaining 11 were divided into eight groups by cross-hybridization. Groups 1-4 contain cDNAs that originate from single-copy X-linked genes in YAC2. Each has an open reading frame of > 500 bp and detects one or more transcripts on a Northern blot. The gene for each was sublocalized and ordered in YAC2. The cDNAs in groups 5-8 contained two or more Alu sequences, had no open reading frames, and did not detect transcripts. The cDNAs from groups 1-4 provide expressed sequence tags and identify candidate genes for the genetic disorders that map to this region.
Geraghty
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Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Brody
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LC
Martin
L S
LS
Marble
M
M
Kearns
W
W
Pearson
P
P
Monaco
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AP
Lehrach
H
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Valle
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D
D015825:Chromosomes, Fungal; D004247:DNA / Q000302:isolation & purification*; D015723:Gene Library; D005796:Genes; D015894:Genome, Human; D006801:Humans; D016366:Open Reading Frames; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014960:X Chromosome
D012333:RNA, Messenger; D004247:DNA; D009953:Ornithine-Oxo-Acid Transaminase