Transforming growth factor(TGF)-beta 1 induces leukemic cell-growth-promoting activity in fibroblast cells.
The production of the leukemic cell-growth-promoting factor (LGF) in TGF-beta 1-treated fibroblast cells was studied. BALB/c3T3 mouse fibroblast (3T3) cells cultured in Eagle's medium containing a low concentration of TGF-beta 1 (0.04-1 ng/ml) secreted 3-5 times more LGF than the cells cultured in the absence of TGF-beta 1. The amount of LGF secretion was dose-dependent on the concentration of post-cultured medium and time-dependent after the addition of TGF-beta 1. Similar findings were obtained in human diploid fibroblasts, WI-38 cells. LGF is a 18KD glycoprotein that is acid-stable but heat-unstable.
Komatsu
K
K
Department of Tumor Biochemistry, Center for Adult Diseases, Osaka, Japan.
Nakamura
H
H
Akedo
H
H
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006133:Growth Substances / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008168:Lung; D051379:Mice; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016212:Transforming Growth Factor beta; C047526:leukemia growth-promoting factor; D003513:Cycloheximide