[Changes in the rates of glucose consumption and lactate release by cells in perfused and nonperfused cultures].
The energetic state of Chinese hamster fibroblasts was investigated under stationary cultural conditions and under condition of culture medium perfusion immediately above the cells. Specific rates of glucose utilization and lactate formation under the former conditions (1.88 +/- 0.2) x 10(-13) and (4.3 +/- 0.56) x 10(-13) Mole/cell/h at the logarithmic growth phase, and (0.21 +/- 0.08) x 10(-13) and (0.58 +/- 0.06) x 10(-13) Mole/cell/h at the stationary phase, respectively. In the perfused culture, specific rates of glucose utilization and formation of lactate are (4.86 +/- 0.56) x 10(-13) and (11.0 +/- 1.8) x 10(-13) Mole/cell/h at the logarithmic growth phase, and (1.57 +/- 0.14) x 10(-13) and (4.11 +/- 0.5) x 10(-13) Mole/cell/h at the stationary phase, respectively. It has been proposed that under conditions of stationary culture the fall of the rates, as the culture reaches the survival phase, is due to diffusion-dependent limitations of mass transfer between the medium and the culture. Under perfusion conditions, the fall of the rates can be explained by some deficiency of necessary components and by excessive amounts of metabolic products in the multilayer structure.
Berestovskaia
N G
NG
Akatov
V S
VS
Lavrovskaia
V P
VP
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D010477:Perfusion / Q000295:instrumentation / Q000379:methods
D007773:Lactates; D019344:Lactic Acid; D005947:Glucose
Izmenenie skorosteĭ potrebleniia gliukozy i vydeleniia laktata kletkami v perfuziruemoĭ i neperfuziruemoĭ kul'turakh.