title: Tau protein kinase II has a similar characteristic to cdc2 kinase for phosphorylating neurofilament proteins.
abstract: Tau protein kinase II purified from a bovine brain tau protein fraction (Ishiguro, K., Takamatsu, M., Tomizawa, K., Omori, A., Takahashi, M., Arioka, M., Uchida, T., and Imahori, K. (1992) J. Biol. Chem. 267, 10897-10901) was shown to have a similar substrate specificity to cdc2 kinase in that both phosphorylate neurofilament (NF) proteins. Tau protein kinase II recognized the dephosphorylated form of the heavy subunit of NF (NF-H) as a predominant substrate. The substrate was phosphorylated to the same extent with tau protein kinase II as with cdc2 kinase. Upon phosphorylation, the electrophoretic mobility of the NF-H on SDS-polyacrylamide gel electrophoresis changed to the position of the phosphorylated form. A synthetic peptide containing a KSPXK sequence was by far a better substrate for tau protein kinase II than that containing a KSPXX sequence, as was also observed with cdc2 kinase. NF-H lost its microtubule-associating ability upon phosphorylation with tau protein kinase II as well as with cdc2 kinase. Although anti-PSTAIR antibody (PSTAIR is an amino acid sequence commonly found in cdc2 and several cdc2-related kinases) failed to react with tau protein kinase II, tau protein kinase II bound to p13suc1-Sepharose beads (p13suc1 is a yeast protein known to bind to cdc2 kinase).
authors.affiliation: Laboratory of Cell and Developmental Biology, Faculty of Biosciences, Tokyo Institute of Technology, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hisanaga
authors.forename: K
authors.initials: K
authors.lastname: Ishiguro
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: E
authors.initials: E
authors.lastname: Okumura
authors.forename: T
authors.initials: T
authors.lastname: Okano
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002417:Cattle; D051360:Cyclin-Dependent Kinase 5; D008969:Molecular Sequence Data; D016900:Neurofilament Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D016900:Neurofilament Proteins; D010455:Peptides; D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; D016203:CDC2 Protein Kinase; C079134:tau protein kinase II