title: Uniform detection of HIV-1 in alveolar macrophages of pediatric but not adult AIDS patients.
abstract: Manifestations of pulmonary disease in pediatric AIDS patients differ from those in adults. To evaluate whether differences in the frequency of alveolar macrophage (AM) infection with human immunodeficiency virus type 1 (HIV-1) could account for these clinical distinctions, we undertook a comparative analysis of HIV-1 DNA in AMs from pediatric and adult AIDS patients by enzymatic amplification. A higher frequency of viral DNA detection in pediatric cases (100%) compared with adults (67%) was observed. The sensitivity of detection was 1 viral DNA copy per 4000 AMs; matched peripheral blood mononuclear cells from six of seven pediatric and eight of nine adult patients tested HIV-1 DNA positive. Adult but not pediatric patients exhibited a marked alveolar lymphocytosis, 32% mean lymphocyte count compared with 7.0%, respectively. These results suggest that the burden of HIV-1 in the lungs of pediatric AIDS patients is greater than that in adults.
authors.affiliation: Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital Center, College of Physicians and Surgeons, Columbia University, New York, New York 10019.
authors.forename: T E
authors.initials: TE
authors.lastname: Pearce
authors.forename: M
authors.initials: M
authors.lastname: Nowakowski
authors.forename: E
authors.initials: E
authors.lastname: Eden
authors.forename: Z B
authors.initials: ZB
authors.lastname: Huang
authors.forename: P
authors.initials: P
authors.lastname: Steiner
authors.forename: M
authors.initials: M
authors.lastname: Shahabuddin
authors.forename: M J
authors.initials: MJ
authors.lastname: Potash
authors.forename: D J
authors.initials: DJ
authors.lastname: Volsky
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000328:Adult; D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D016676:Macrophages, Alveolar / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral