title: 7,8-Dihydro-8-oxo-2'-deoxyguanosine present in DNA is not simply an artefact of isolation.
abstract: 7,8-Dihydro-8-oxo-2'-deoxyguanosine (8-oxodG) is present in DNA isolated from human and murine cells. Other studies have reported artificially elevated levels in DNA extracted using phenol-based methods. This report shows that DNA, isolated by different methods, with or without the use of a phenol reagent, contains similar levels of 8-oxodG. This, taken with other studies of human tissues which show varying levels of 8-oxodG, indicates that, with due care in the extraction procedure, biologically significant amounts of this altered base are present in mammalian DNA.
authors.affiliation: London Hospital Medical College, University of London, UK.
authors.forename: G
authors.initials: G
authors.lastname: Harris
authors.forename: S
authors.initials: S
authors.lastname: Bashir
authors.forename: P G
authors.initials: PG
authors.lastname: Winyard
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D016477:Artifacts; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D051379:Mice; D008808:Mice, Inbred CBA
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine