[Combination induction of cell differentiation of HL-60 cells by daidzein (S86019) and BC-4 or Ara-C].
Experiments demonstrated that the cell differentiation of HL-60 cells induced by low concentrations of daidzein (S86019), BC-4 (active principle of Boswellia carterii Birdw) and Ara-C was not impressive. However, when they were used in combination 80% of HL-60 cells exhibited NBT reduction and 82% of the cells showed phagocytosis after four days exposure to daidzein and BC-4. When HL-60 cells were exposed to daidzein and Ara-c, 70% of the cells exhibited NBT reduction and phagocytosis. Flow cytometry indicated that the majority of the cells were blocked at G1 phase under the induction of combination of daidzein with BC-4 or Ara-C.
Jing
Y K
YK
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Han
R
R
D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003561:Cytarabine / Q000494:pharmacology; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D010587:Phagocytosis / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D003561:Cytarabine; C004742:daidzein