Screening of synthetic peptide libraries with radiolabeled acceptor molecules.
A method has been developed for the identification of specific acceptor molecule-binding sequences from a chemically synthesized peptide library. The peptide resin beads, each bead carrying one peptide sequence, are incubated with radiolabeled acceptor molecule and subsequently immobilized in a thin layer of agarose. Resin beads that carry acceptor molecule-specific sequences are identified by autoradiography and subjected to automated gas-phase sequencing. The method was tested by screening a synthetic pentapeptide library with an anti-beta-endorphin monoclonal antibody.
Kassarjian
A
A
Howard Hughes Medical Institute, University of California, San Francisco.
Schellenberger
V
V
Turck
C W
CW
D000595:Amino Acid Sequence; D001345:Autoradiography; D007553:Isotope Labeling / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D011868:Radioisotopes
D010455:Peptides; D011868:Radioisotopes