title: An ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of Ascaris suum.
abstract: Ivermectin has been shown to increase chloride conductances of invertebrate cells. On the muscle cells of the parasitic nematode Ascaris, ivermectin acts as both a GABA receptor antagonist and a chloride channel opener. In this study, ion-sensitive microelectrodes were used to investigate the effect of ivermectin on intracellular Cl- concentration of the somatic muscle bag cells of Ascaris suum. Incubation of muscle cells with ivermectin (10 microM in 1% dimethyl sulphoxide vehicle for 60 min) increased intracellular Cl- by 2.9 mM or 15% compared to controls (P < 0.01, n = 6).
authors.affiliation: Department of Physiology and Pharmacology, University of Southampton, UK.
authors.forename: H R
authors.initials: HR
authors.lastname: Parri
authors.forename: M B
authors.initials: MB
authors.lastname: Djamgoz
authors.forename: L
authors.initials: L
authors.lastname: Holden-Dye
authors.forename: R J
authors.initials: RJ
authors.lastname: Walker
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000187:drug effects / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007477:Ions; D007559:Ivermectin / Q000494:pharmacology*; D008564:Membrane Potentials; D008839:Microelectrodes; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D012680:Sensitivity and Specificity; D014882:Water-Electrolyte Balance / Q000187:drug effects*
chemical_list: D002712:Chlorides; D007477:Ions; D007559:Ivermectin; D004121:Dimethyl Sulfoxide