[Lectin histochemistry of choroidal malignant melanoma].
Tumor specimens of malignant choroidal melanoma from a 50 year-old man were studied with reference to labeling with fluorescence-labeled lectins: wheat germ agglutinin (WGA), specific for sialic acid and N-acetylglucosamine, and Ricinus communis agglutinin-1 (RCA-1), specific for galactose. Epithelioid cells occupying the apex and internal portion of a dome-shaped tumor had intense labeling of WGA but little binding to RCA-1. Spindle-shaped cells in the base of the tumor were labeled moderately with WGA and faintly with RCA-1. Neuraminidase treatment of the specimens led to a marked increase in the RCA-1 binding to both epithelioid and spindle-shaped tumor cells. The results suggest association of the malignancy of choroidal melanoma cells with oligosaccharides of cell membrane-bound glycoconjugates.
Sugata
M
M
Department of Ophthalmology, Kagoshima University Faculty of Medicine, Japan.
Uehara
F
F
Nakashima
Y
Y
Ohba
N
N
D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D002830:Choroid Neoplasms / Q000737:chemistry* / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins; D008297:Male; D008545:Melanoma / Q000737:chemistry* / Q000473:pathology; D008875:Middle Aged; D009844:Oligosaccharides / Q000032:analysis*; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins
D015295:Antigens, Tumor-Associated, Carbohydrate; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014909:Wheat Germ Agglutinins