title: Phenotypic identification of amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae.
abstract: Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, OH 45221-0006.
authors.forename: M J
authors.initials: MJ
authors.lastname: Dorsey
authors.forename: P
authors.initials: P
authors.lastname: Hoeh
authors.forename: C E
authors.initials: CE
authors.lastname: Paquin
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005784:Gene Amplification; D005800:Genes, Fungal; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D000426:Alcohol Dehydrogenase