title: Chromosomal structure and expression of the human OTF1 locus encoding the Oct-1 protein.
abstract: The genomic structure of the POU domain containing oct-1 gene (OTF1 locus) coding region has been determined using human DNA recombinant bacteriophage and a yeast artificial chromosome clone. The gene is encoded by 16 exons spanning over 150 kb, and the Oct-1 protein reading frame has been extended to 766 amino acids. The exonic structure has been compared to the mouse Oct-2 protein and reveals a conservation of exon-intron boundaries as well as protein sequence similarity. To provide insight into Oct-1 control of transcriptional regulation during the cell-cycle the expression of the oct-1 gene was examined during cellular DNA replication and shows that the steady-state level of the oct-1 mRNA is not S-phase regulated.
authors.affiliation: Centre for Molecular Biology and Biotechnology, University of Queensland, Australia.
authors.forename: R A
authors.initials: RA
authors.lastname: Sturm
authors.forename: J L
authors.initials: JL
authors.lastname: Cassady
authors.forename: G
authors.initials: G
authors.lastname: Das
authors.forename: A
authors.initials: A
authors.lastname: Romo
authors.forename: G A
authors.initials: GA
authors.lastname: Evans
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D015825:Chromosomes, Fungal; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D005786:Gene Expression Regulation; D015723:Gene Library; D005796:Genes; D015894:Genome, Human; D006657:Histones / Q000096:biosynthesis / Q000235:genetics; D051863:Host Cell Factor C1; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000235:genetics; D016196:S Phase; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; D006657:Histones; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C494165:POU2F1 protein, human; C058435:POU2F2 protein, human; C489206:Pou2f2 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors