title: Metanephric transforming growth factor-beta 1 regulates nephrogenesis in vitro.
abstract: Development of the metanephric kidney during embryogenesis is regulated by a number of polypeptide growth factors of renal origin. We have defined previously a role for insulin-like growth factors (IGF) I and II and for transforming growth factor (TGF)-alpha. To delineate the effect of TGF-beta 1, on renal organogenesis, we cultured metanephroi surgically dissected from 13-day-old rat embryos in serum-free chemically defined media. TGF-beta 1 mRNA was present in kidneys from 13-day-old rat embryos, and positive immunostaining for TGF-beta 1 could be demonstrated in cultured metanephroi. However, TGF-beta bioactivity could not be detected in media obtained from the metanephroi. Addition of 10(-9) M TGF-beta 1 to cultures inhibited tubulogenesis, but had no effect on synthesis of IGF-I or -II. Addition of anti-TGF-beta 1 antibodies to cultures accelerated tubulogenesis within the metanephric blastema. These findings establish the potential for TGF-beta 1 production within the rat metanephros during development in vivo. It is possible that this peptide exerts a negative control on the process of tubulogenesis within metanephric blastema and in this manner acts to shape the architecture of mature kidney.
authors.affiliation: George M. O'Brien Kidney and Urological Diseases Center, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: S A
authors.initials: SA
authors.lastname: Rogers
authors.forename: G
authors.initials: G
authors.lastname: Ryan
authors.forename: A F
authors.initials: AF
authors.lastname: Purchio
authors.forename: M R
authors.initials: MR
authors.lastname: Hammerman
mesh_terms: D000818:Animals; D003470:Culture Media; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D007668:Kidney / Q000196:embryology*; D007684:Kidney Tubules / Q000196:embryology; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000502:physiology*
chemical_list: D003470:Culture Media; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II