[HPLC analysis of alkaloids in Sophora flavescens Ait].
In this paper, a HPLC method was developed for the determination of alkaloids in Sophora flavescens Ait. Experimental evidences indicate that a system of a LiChrosorb-NH2 column (4.0 mm x 250 mm) as stationary phase and CH3CN--H3PO4(pH2)--CH3CH2OH(80:8:10) as mobile phase can separate the five alkaloids, sophocarpine, matrine, sophoridine, oxysophcarpine and oxymatrine very well. This method is very easy and efficient and takes only 15 min for one run.
Jin
L X
LX
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Cui
Y Y
YY
Zhang
G D
GD
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry*; D010946:Plants, Medicinal; D011807:Quinolizines; D000093842:Matrines
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; C035933:sophocarpine; D000093842:Matrines