Induction of stress proteins in mouse peritoneal macrophages by oxidized low-density lipoprotein.
The synthesis of 23-kDa and 34-kDa proteins in mouse peritoneal macrophages was enhanced when they were incubated with oxidatively modified low-density lipoprotein (LDL). This response required the oxidation of the lipid of the LDL, because native LDL had little effect and acetylated LDL enhanced the synthesis of 34-kDa protein only slightly. Since macrophages can bind and sequester oxidized LDL, they are subjected to an oxidative stress. The results suggest that the 34-kDa protein, which is identical with heme oxygenase, and the 23-kDa protein serve as stress protein that might afford protection against the oxidative stress.
Yamaguchi
M
M
Department of Biochemistry, Tsukuba University Medical School, Ibaraki, Japan.
Sato
H
H
Bannai
S
S
D000818:Animals; D002478:Cells, Cultured; D003553:Cystine / Q000378:metabolism; D004589:Electrophoresis, Disc; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013462:Sulfur Radioisotopes
D006360:Heat-Shock Proteins; D008077:Lipoproteins, LDL; D013462:Sulfur Radioisotopes; D003553:Cystine; D008715:Methionine