title: Partial cloning of cDNA for hamster liver cholesterol 7 alpha-hydroxylase.
abstract: A partial cDNA clone encoding cholesterol 7 alpha-hydroxylase (pHA 7 alpha-3) was isolated from a hamster liver cDNA library using rat P-450 ch7 cDNA (p 7 alpha-11) as a probe. In comparison with the complete base sequence of p 7 alpha-11, pHA 7 alpha-3 lacked about 500 base pairs at the 5' end. Circadian rhythms of mRNA for hamster liver 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol 7 alpha-hydroxylase were investigated with cDNA clones encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase (pRed-227) and pHA 7 alpha-3. Typical circadian rhythms of mRNA for both enzymes and a time lag between the two circadian rhythms were observed. The circadian rhythm of activity for each enzyme corresponded to that of each mRNA.
authors.affiliation: First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Kurushima
authors.forename: M
authors.initials: M
authors.lastname: Saeki
authors.forename: H
authors.initials: H
authors.lastname: Amioka
authors.forename: J
authors.initials: J
authors.lastname: Kurokawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kuga
authors.forename: S
authors.initials: S
authors.lastname: Nomura
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkura
authors.forename: H
authors.initials: H
authors.lastname: Ohtani
authors.forename: M
authors.initials: M
authors.lastname: Noshiro
mesh_terms: D000818:Animals; D001483:Base Sequence; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics* / Q000302:isolation & purification; D002940:Circadian Rhythm / Q000235:genetics; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D002790:Cholesterol 7-alpha-Hydroxylase