Erythroblasts as an index of initial stage of 2,7-FAA, ENU and BNU-induced rat leukemia.
The appearance of orthochromatic erythroblasts in the peripheral blood was evaluated as an index of the initial stage of leukemia induced in rats by the oral administration of N,N'-2,7-fluorenylenebisacetamide, 1-ethyl-1-nitrosourea and 1-butyl-1-nitrosourea. Solitary leukemia lesions found mainly in the bone marrow and composed of one or a few nodular foci were found by using erythroblasts as an index. The time interval from the first appearance of erythroblasts to autopsy was short in solitary lesions, slightly longer in scattered lesions, and even longer in diffuse leukemia lesions. Although the appearance of orthochromatic erythroblasts in the peripheral blood was due to several causes other than leukemia, this appearance was useful as an index of the initial stage of chemically induced leukemia in rats.
Takayama
S
S
Department of Pathology, Saitama Cancer Center, Japan.
D015073:2-Acetylaminofluorene / Q000031:analogs & derivatives; D000818:Animals; D002273:Carcinogens; D004900:Erythroblasts / Q000473:pathology*; D005038:Ethylnitrosourea; D005260:Female; D007942:Leukemia, Experimental / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D009607:Nitrosourea Compounds; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012720:Severity of Illness Index
D002273:Carcinogens; D009607:Nitrosourea Compounds; C100105:2,7-diacetylaminofluorene; C002738:N-nitrosobutylurea; D015073:2-Acetylaminofluorene; D005038:Ethylnitrosourea