[Antimicrobial activity of new quinolones against Mycobacterium avium and Mycobacterium intracellulare determined by the dilution methods using 7H11 agar and Ogawa egg media].
Various new quinolones were measured for their in vitro antimicrobial activities against Mycobacterium avium and M. intracellulare by the two-fold dilution methods using two types of media, 7H11 agar medium and 1% Ogawa egg medium. The MIC90s of test quinolones determined on 7H11 agar medium and Ogawa egg medium were as follows. M. avium (20 strains): ofloxacin (OFLX), 50 and 50 micrograms/ml; ciprofloxacin (CPFX), 12.5 and 25 micrograms/ml; sparfloxacin (SPFX), 6.25 and 12.5 micrograms/ml; fleroxacin (FLRX), 50 and 50 micrograms/ml; Y-26611, 100 and 100 micrograms/ml; OPC-17116, 12.5 and 50 micrograms/ml, for 7H11 agar and Ogawa egg medium, respectively. M. intracellulare (20 strains): OFLX, 50 and 50 micrograms/ml; CPFX, 25 and 25 micrograms/ml; SPFX, 12.5 and 12.5 micrograms/ml; FLRX, 50 and 50 micrograms/ml; Y-26611, > 100 and > 100 micrograms/ml; OPC-17116, 12.5 and > 100 micrograms/ml, for 7H11 agar and Ogawa egg medium, respectively. Thus, the MIC values determined by the 7H11 agar medium were comparable to those by the 1% Ogawa egg medium, except for OPC-17116. Moreover, from the above MIC values, it can be regarded that the potencies of in vitro antimicrobial activity of test quinolones against M. avium are in the order of SPFX > CPFX > or = OPC-17116 > or = OFLX > or = FLRX > Y-26611 and those against M. intracellulare are in the order of SPFX > OPC-17116 not equal to CPFX > OFLX = FLRX > Y-26611. There was found the tendency that M. avium has somewhat higher susceptibility to these quinolones as compared to M. intracellulare.
Tomioka
H
H
Department of Microbiology and Immunology, Shimane Medical University, Japan.
Sato
K
K
Saito
H
H
D042462:4-Quinolones; D000362:Agar; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D007201:Indicator Dilution Techniques; D008826:Microbial Sensitivity Tests; D009162:Mycobacterium avium / Q000187:drug effects*; D015269:Mycobacterium avium Complex / Q000187:drug effects*
D042462:4-Quinolones; D000890:Anti-Infective Agents; D003470:Culture Media; D000362:Agar