[A comparative assessment of the biochemical criteria of provision of the body with thiamine].
Activity of transketolase in erythrocyte hemolysates, estimated by means of measurement of sedoheptulose-7-phosphate content without preinactivation of transaldolase, proved to be 3-4-fold lower as compared with the values observed after preinactivation of transaldolase. The values of thiamine diphosphate (TDP) effect did not depend on conditions of the transketolase reaction, as well as on the colorimetric procedure used for determination of sedoheptulose-7-phosphate. A high inverse correlation was found between the value of TDP effect and an excretion of thiamine with urine in adult patients and in children of 9-13 years old. Normal excretion of thiamine with urine constituted 11-12 micrograms/h in the children of this age as judged from the TDP effect-thiamine excretion in children of this age.
Sokol'nikov
A A
AA
Kodentsova
V M
VM
Isaeva
V A
VA
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D009752:Nutritional Status; D013831:Thiamine / Q000008:administration & dosage* / Q000652:urine; D014153:Transaldolase / Q000037:antagonists & inhibitors; D014174:Transketolase / Q000378:metabolism
D014174:Transketolase; D014153:Transaldolase; D013831:Thiamine
Sravnitel'naia otsenka biokhimicheskikh kriteriev obespechennosti organizma tiaminom.