Crystallization and preliminary crystallographic study of citrate synthase from the thermophilic Archaeon Thermoplasma acidophilum.
Single crystals of citrate synthase from the Archaeon Thermoplasma acidophilum were obtained in two forms using the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group P222(1), with unit cell dimensions a = 80.9 A, b = 103.8 A, c = 98.3 A and one dimer in the asymmetric unit. Type 2 crystals belong to the monoclinic space group P2(1), with unit cell dimensions a = 53.8 A, b = 173.8 A, c = 86.7 A and beta = 97.1 degrees and two dimers in the asymmetric unit.
Russell
R J
RJ
Department of Biochemistry, University of Bath, Claverton Down, U.K.
Byrom
D
D
Danson
M J
MJ
Hough
D W
DW
Taylor
G L
GL
D002950:Citrate (si)-Synthase / Q000302:isolation & purification / Q000648:ultrastructure*; D003461:Crystallography; D011487:Protein Conformation; D013822:Thermoplasma / Q000201:enzymology*; D014961:X-Ray Diffraction
D002950:Citrate (si)-Synthase