Cell-associated and soluble phospholipases A2 increase during carrageenan and zymosan-induced pleurisy in rat.
Extra-cellular and cell-associated Ca(2+)-dependent phospholipases A2 and released thromboxane B2 were correlated to exudation and cell migration during rat pleurisy induced by carrageenan or zymosan. Extra-cellular phospholipase A2 was delayed with respect to acute inflammation, while cell-associated phospholipase A2 closely correlated with cell migration and thromboxane B2 levels. This confirms that the subcellular localization of phospholipases A2 is linked to their physiological action and, in particular, suggests that the cell-associated, rather than the extracellular enzyme, accounts for the production of eicosanoids.
Tesson
F
F
Unités des Venins et de Pharmacologie Cellulaire, Unité Associée Institut Pasteur/INSERM U-285, Paris, France.
Ruffié
C
C
Hidi
R
R
e Silva
P M
PM
Vazeux
G
G
Vargaftig
B B
BB
Bon
C
C
D000818:Animals; D015415:Biomarkers; D002351:Carrageenan; D004789:Enzyme Activation / Q000187:drug effects; D005110:Extracellular Space / Q000201:enzymology; D005122:Exudates and Transudates / Q000201:enzymology; D007700:Kinetics; D008297:Male; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010998:Pleurisy / Q000139:chemically induced / Q000201:enzymology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013929:Thromboxane B2 / Q000378:metabolism; D015054:Zymosan
D015415:Biomarkers; D013929:Thromboxane B2; D002351:Carrageenan; D015054:Zymosan; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054467:Phospholipases A2