Stimulatory effect of the maize HMGa protein on reporter gene expression in maize protoplasts.
The high mobility group (HMG) proteins represent a class of chromosomal non-histone proteins with an assumed influence on transcription. In this context, the effect of the maize HMGa protein on reporter gene expression was examined. Transient co-transformation experiments in maize protoplasts with plasmid constructs directing the synthesis of the maize HMGa protein and with a luciferase reporter plasmid demonstrated a stimulatory effect of the HMGa protein on the reporter gene expression. Additional experiments with HMGa deletion constructs indicated that the HMG-Box DNA-binding motif is important for the observed effect, while the acidic carboxy-terminal domain of the HMGa protein appears to be dispensable.
Grasser
K D
KD
Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, Germany.
Hetz
W
W
Griess
E A
EA
Feix
G
G
D015971:Gene Expression Regulation, Enzymologic; D006609:High Mobility Group Proteins / Q000494:pharmacology*; D008156:Luciferases / Q000235:genetics; D010940:Plant Proteins; D010957:Plasmids; D011523:Protoplasts; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics*
D006609:High Mobility Group Proteins; D010940:Plant Proteins; D008156:Luciferases