Study of localization of the protein-synthesizing machinery along actin filament bundles.
Indirect immunofluorescent microscopy was used to study the distribution of elongation factor 2 (eEF-2) in fixed human skin diploid and mouse embryo fibroblasts. It was found earlier that some of the eEF-2, ribosomes and initiation factor 2 (eIF-2) are co-localized with a part of the actin microfilament bundles in these cells (Gavrilova et al., 1987; Shestakova et al., 1991). Here it has been shown that inhibition of protein synthesis either by inactivation of eEF-2 itself with diphtheria toxin or by inactivation of ribosomes with ricin does not abolish the distribution of eEF-2 along the actin microfilament bundles. At the same time, the disassembly of actin microfilaments by cytochalasin D results also in the disappearance of eEF-2- carrying threads. This means that the eEF-2-carrying threads do not exist per se, and that the organization of eEF-2 in visible "filaments" depends upon the integrity of the actin cytoskeleton.
Shestakova
E A
EA
Institute of Protein Research, Academy of Sciences of Russia, Pushchino, Moscow Region.
Motuz
L P
LP
Minin
A A
AA
Gavrilova
L P
LP
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D000199:Actins / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D004167:Diphtheria Toxin / Q000494:pharmacology; D004622:Embryo, Mammalian; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007930:Leucine / Q000378:metabolism; D051379:Mice; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000378:metabolism*; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D000199:Actins; D004167:Diphtheria Toxin; D015852:Eukaryotic Initiation Factor-2; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D015638:Cytochalasin D; D007930:Leucine