title: Direct demonstration of MHC class II surface expression on murine pre-B cells.
abstract: Using multi-parameter flow cytometry, MHC class II (Ia) antigens were found to be expressed at readily detectable levels on the majority of surface IgM- B lineage cells in the bone marrow of adult mice. Approximately 90% of the pre-B cells (B220+IgM-) in BALB/c bone marrow (BM) were MCH class II positive, while in C57BL/6 this figure was somewhat less, approximately 60%. Analysis of adult BM for the correlated expression of Ia both with heat stable antigen (HSA) and BP-1 on B lineage cells revealed in both cases a small fraction of cells expressing either the HSA or BP-1 but lacking Ia. This result suggests that the onset of expression of both HSA and BP-1 precede that of Ia in B cell development. When the B220loCD43+ pro-B cell compartment from BALB/c BM was analysed for the expression of Ia very few, if any, cells were found to be positive. Of the remaining B220+ cells, > 90% express detectable Ia. Thus the onset of Ia expression would appear to be soon after the pro-B cell compartment. The results presented here resolve the previous discrepancy between human and murine pre-B cell differentiation of when Ia is first expressed, and additionally, by identifying a new pre-B cell antigen, allow for the refinement of existing schemes of murine B cell development.
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Tarlinton
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D001483:Base Sequence; D001854:Bone Marrow Cells; D018076:DNA, Complementary; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data
chemical_list: D018076:DNA, Complementary; D000949:Histocompatibility Antigens Class II; D007075:Immunoglobulin M