title: Simple phagemid-based system for generating allele replacements in Escherichia coli.
abstract: We describe a phagemid-based system for rapidly generating chromosomal allele replacements in two steps. The system utilizes simple vector-determined selection procedures for each step and requires only that the target strain be male and sup+ (nonsuppressing for amber mutations).
authors.affiliation: Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4960.
authors.forename: S
authors.initials: S
authors.lastname: Slater
authors.forename: R
authors.initials: R
authors.lastname: Maurer
mesh_terms: D002876:Chromosomes, Bacterial; D003090:Coliphages / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic