title: CDw50 and ICAM-3: two names for the same molecule.
abstract: CDw50 differentiation antigen is a molecule broadly expressed on hematopoetic cells but not on other cells. Previous experiments showed that CDw50 monoclonal antibodies (mAb) inhibited primary mixed lymphocyte culture (MLC). To understand the function of CDw50 better, we purified it and obtained peptide sequence. At the same time, intercellular adhesion molecule (ICAM)-3, the third ligand of lymphocyte function-associated molecule 1, was described by mAb and subsequent cDNA cloning. Immunochemical, functional, and protein sequencing studies show that ICAM-3 and CDw50 are the same glycoprotein, a 120-kDa surface molecule with presumably an important role in the immune responses.
authors.affiliation: Servei d'Immunologia, Hospital Clínic, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Juan
authors.forename: R
authors.initials: R
authors.lastname: Vilella
authors.forename: J
authors.initials: J
authors.lastname: Mila
authors.forename: J
authors.initials: J
authors.lastname: Yagüe
authors.forename: A
authors.initials: A
authors.lastname: Miralles
authors.forename: K S
authors.initials: KS
authors.lastname: Campbell
authors.forename: R J
authors.initials: RJ
authors.lastname: Friedrich
authors.forename: J
authors.initials: J
authors.lastname: Cambier
authors.forename: J
authors.initials: J
authors.lastname: Vives
authors.forename: A R
authors.initials: AR
authors.lastname: De Fougerolles
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000737:chemistry* / Q000276:immunology; D000943:Antigens, Differentiation / Q000737:chemistry* / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000276:immunology; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D016415:Sequence Alignment; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; C483616:ICAM3 protein, human; D010455:Peptides