[Can ulinastatin be an effective inhibitor of human polymorphonuclear granulocyte elastase under severe stressed state?].
The inhibitory effect of ulinastatin (UST), an intrinsic human trypsin inhibitor was investigated on the activity of polymorphonuclear granulocyte elastase (PMNE) with or without alpha 1-protease inhibitor (alpha 1-PI) using the in vitro models. The results of the dodecyl-sulfate-electrophoresis (SDS-PAGE), indicated that splitting-action of crude granulocyte enzyme solution on the plasma fibronectin was inhibited by concentration-dependent UST of an equivalent value for treatment of stressed state. The PMNE-UST complex was competitively replaced by PMNE-alpha 1-PI complex in vitro models of inflammatory focus and circulation, hence UST was weaker than alpha 1-PI in its binding-affinity for PMNE. The PMNE activity was directly inhibited by UST in the inflammatory focus and circulation with or without alpha 1-PI.
Ishikawa
A
A
Department of Surgery, University of Tsukuba, Japan.
Fukao
K
K
Tsuji
K
K
Osada
A
A
Yamamoto
Y
Y
Iwasaki
Y
Y
D001667:Binding, Competitive; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000378:metabolism; D006023:Glycoproteins / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000201:enzymology; D019272:Leukocyte Elastase; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013312:Stress, Physiological / Q000201:enzymology*; D000515:alpha 1-Antitrypsin / Q000378:metabolism
D005353:Fibronectins; D006023:Glycoproteins; D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; C028665:urinastatin