title: Heterogeneous effects of histamine on proliferation of lung- and blood-derived T-cell clones from healthy and asthmatic persons.
abstract: We have studied the effects of histamine on the proliferation and the intracellular cyclic adenosine monophosphate (cAMP) levels of T-lymphocyte clones (TLC) generated from bronchoalveolar lavage fluid (BALF) or peripheral blood (PB) from healthy and asthmatic persons. TLC from either compartment and from both groups of donors were heterogeneous in their response to histamine. In BALF-derived TLC, three types of responses were observed: histamine inhibited, stimulated, or did not modulate the anti-CD3-induced proliferation. Histamine directly and dose dependently inhibited the anti-CD3-induced proliferation of six (two asthmatic) of 12 CD4+ BALF TLC, stimulated two BALF TLC (both nonasthmatic), and did not modulate the proliferation of four BALF TLC. The maximal inhibition was 70%, the maximal stimulation 200%, both at 10(-3) M histamine. The stimulation of proliferation was associated with increased interleukin-2 (IL-2) production, whereas the inhibition of proliferation was associated with decreased IL-2 production and downregulation of IL-2 receptor expression. The inhibitory effects could be partly reversed by H2-receptor antagonists and could be mimicked by an H2-receptor agonist. In contrast, the stimulatory effect was not reversed or mimicked by H1 or H2 antagonists or agonists. The majority of CD4+ TLC responded to histamine with a rise in the intracellular cAMP levels. A rise in cAMP, however, was often but not always associated with an inhibition of proliferation. In addition, stimulation of proliferation occurred in the absence of a rise in cAMP. We compared cAMP rises in panels of TLC obtained with high cloning efficiencies from the PB from a healthy person and from an asthmatic person.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pulmonology, Academic Medical Centre, University of Amsterdam, The Netherlands.
authors.forename: B E
authors.initials: BE
authors.lastname: Hol
authors.forename: F H
authors.initials: FH
authors.lastname: Krouwels
authors.forename: B
authors.initials: B
authors.lastname: Bruinier
authors.forename: R
authors.initials: R
authors.lastname: Lutter
authors.forename: A
authors.initials: A
authors.lastname: Bast
authors.forename: E A
authors.initials: EA
authors.lastname: Wierenga
authors.forename: H M
authors.initials: HM
authors.lastname: Jansen
authors.forename: T A
authors.initials: TA
authors.lastname: Out
mesh_terms: D001249:Asthma / Q000276:immunology*; D001769:Blood; D002455:Cell Division; D002999:Clone Cells; D006632:Histamine / Q000502:physiology*; D007376:Interleukin-2 / Q000096:biosynthesis; D008168:Lung / Q000166:cytology / Q000276:immunology*; D012016:Reference Values; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D007376:Interleukin-2; D006632:Histamine