Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.
A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.
Lee
C H
CH
Department of Pathology, Indiana University School of Medicine, Indianapolis 46202.
Bauer
N L
NL
Shaw
M M
MM
Durkin
M M
MM
Bartlett
M S
MS
Queener
S F
SF
Smith
J W
JW
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004271:DNA, Fungal / Q000235:genetics; D004622:Embryo, Mammalian; D005069:Evaluation Studies as Topic; D005260:Female; D005347:Fibroblasts; D006801:Humans; D008168:Lung; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods*; D011010:Pneumocystis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
D004271:DNA, Fungal