Electrophoretic fractionation of 5'-nucleotidase.
Human isonucleotidases were separated by electrophoresis on a cellulose acetate membrane. Three 5'-nucleotidase forms, NTP1, NTP2, and NTP3, were resolved with this method and quantified by densitometry. The procedure was not only simple and rapid but also sufficiently precise (between-run CV < 20%) and sensitive (detected nucleotidase fractions of > 0.5 U/L). The effects of various treatments (heat, neuraminidase, glycosidases, proteases, lectins, and detergents) on the electrophoretic pattern of 5'-nucleotidase were studied. NTP1 (mean 12% of total 5'-nucleotidase, SD 5%), NTP2 (mean 30%, SD 8%), and NTP3 (mean 58%, SD 8%) were found in all normal persons studied. The increase in total 5'-nucleotidase in patients with hepatobiliary disease was mainly due to the NTP1 isoform.
Panteghini
M
M
Laboratorio Analisi Chimico-Cliniche, Spedali Civili, Brescia, Italy.
D015720:5'-Nucleotidase / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001649:Bile Duct Diseases / Q000201:enzymology; D004588:Electrophoresis, Cellulose Acetate / Q000706:statistics & numerical data; D010450:Endopeptidases / Q000494:pharmacology; D005260:Female; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D008107:Liver Diseases / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D009439:Neuraminidase / Q000494:pharmacology; D017830:Octoxynol / Q000494:pharmacology; D012680:Sensitivity and Specificity; D014018:Tissue Distribution; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D007527:Isoenzymes; D014909:Wheat Germ Agglutinins; D017830:Octoxynol; D015720:5'-Nucleotidase; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D010450:Endopeptidases