Effect of PAF on human lymphocyte membranes: a fluorescence study.
We have studied the effect of platelet-activating factor (PAF) on the physico-chemical organization of human lymphocyte plasma membranes by measuring the steady-state fluorescence anisotropy and the fluorescence decay of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in lymphocyte plasma membranes. PAF induced a time-limited and significant increase of the lipid order in the exterior part of the membrane and a decrease in membrane heterogeneity. These changes were blocked in the presence of PAF antagonist L-659,989. The results indicate that the observed changes in the physico-chemical properties of the lymphocyte plasma membranes may be attributed to a PAF-receptor interaction.
Kantar
A
A
Department of Pediatrics, University of Ancona, Italy.
Giorgi
P L
PL
Fiorini
R
R
D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004161:Diphenylhexatriene / Q000031:analogs & derivatives; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D005663:Furans / Q000494:pharmacology; D006207:Half-Life; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry* / Q000187:drug effects; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D005456:Fluorescent Dyes; D005663:Furans; D010972:Platelet Activating Factor; C054893:L 659989; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene