Solid-phase synthesis of [Ala1,3,11,15]-endothelin-1 by a modified double-coupling procedure.
[Ala1,3,11,15]-Endothelin-1, a linear analogue of endothelin-1 in which alanines replace the cystine residues, has been prepared by solid-phase synthesis in approximately 17% yield. Fmoc amino acids were coupled using an economical modified double-coupling (symmetrical anhydride followed by O-benzotriazolyl ester) procedure under fully automated conditions. Conditions for synthesis and purification were closely monitored and should be applicable to other endothelin analogues.
Green
J
J
Marion Merrell Dow Research Institute, Cincinnati, Ohio 45215.
D000595:Amino Acid Sequence; D016232:Endothelins / Q000138:chemical synthesis*; D008969:Molecular Sequence Data
D016232:Endothelins; C061779:endothelin 1, Ala(1,3,11,15)-