Influence of anesthetic regimens on intestinal absorption in rats.
We compared the influence of anesthetic regimens using urethane (U), pentobarbital (P), ether (E), and ketamine/midazolam (K) on the intestinal absorption of several probes using a single-pass perfusion technique in rats. The selected probes were D-glucose (1 mM) for the resistance of the unstirred water layer (UWL), D-glucose (100 mM) for the capacity of carrier-mediated D-glucose transport, L-glucose, and urea for membrane-limited passive transport, and tritiated water (3H2O) for blood flow at the absorption site. The absorbed fraction of D-glucose (1 mM) was the smallest for U and the largest for P, suggesting that the resistance of UWL is the largest for U and the smallest for P. The absorbed fraction of D-glucose (100 mM) was the largest for P (U = E = K < P), suggesting a higher capacity of carrier-mediated D-glucose transport for P. The absorbed fraction of urea was similar for all anesthetics, while that of L-glucose was the smallest for K (U = P = E > K). Although the results for these two markers of membrane-limited passive transport were inconsistent, the passive permeability of the intestinal membrane may be lower when treating with K. The intestinal absorptions of D-glucose (1 and 100 mM), L-glucose, and urea were, in general, lower with any of the anesthetics than under nonanesthesia (N), suggesting increased resistance of UWL and decreased intestinal membrane permeability by carrier-mediated and passive transport under anesthesia. The only exception was the absorption of D-glucose (100 mM) under P, which was comparable to that under N.(ABSTRACT TRUNCATED AT 250 WORDS)
Yuasa
H
H
Faculty of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan.
Matsuda
K
K
Watanabe
J
J
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D004987:Ethers / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects*; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D008874:Midazolam / Q000494:pharmacology; D010424:Pentobarbital / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000378:metabolism; D014520:Urethane / Q000494:pharmacology
D000777:Anesthetics; D004987:Ethers; D014520:Urethane; D007649:Ketamine; D014508:Urea; D010424:Pentobarbital; D005947:Glucose; D008874:Midazolam