HELLP syndrome-like biochemical parameters obtained with endothelin-1 injections in rabbits.
We studied the effects of bolus injections and infusion of endothelin-1 (ET-1) in female rabbits by measuring serum glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LDH), antithrombin III (AT-III), thrombin antithrombin (TAT) complexes, platelet counts and indirect bilirubin. Two successive bolus doses of 0.125 and 0.25 nmol/kg of ET-1 with an interval of 30 min were given to conscious non-pregnant female rabbits (n = 8). GOT, GPT and LDH were found to be significantly increased after injections of ET-1 (p < 0.02, p < 0.04 and p < 0.05, respectively). The percent AT-III activity decreased significantly (p < 0.005). Vasospasm of the hepatic artery was demonstrated by angiography with the same bolus doses in rabbits. There was also an increase in GOT (p < 0.003), GTP (p < 0.05), LDH (p < 0.007), indirect bilirubin (p > 0.07) and TAT complexes (p < 0.02) and a decrease in AT-III (p < 0.03) and platelet counts (p < 0.02) in rabbits (n = 10) after 24 h of continuous infusion of ET-1 (0.6 nmol/kg/h). Histological examination of rabbit liver tissues showed varying degrees of ischemic necrosis of hepatocytes. Thus this study suggests that exogenously administered ET-1 causes vasospasm and liver ischemia resulting in HELLP syndrome-like blood parameters in rabbits.
Halim
A
A
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Japan.
Kanayama
N
N
Maehara
K
K
Takahashi
M
M
Terao
T
T
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000990:Antithrombin III / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D016232:Endothelins / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D017359:HELLP Syndrome / Q000097:blood* / Q000139:chemically induced* / Q000503:physiopathology; D006499:Hepatic Artery / Q000000981:diagnostic imaging / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000473:pathology; D010976:Platelet Count; D011247:Pregnancy; D011817:Rabbits; D011859:Radiography; D013917:Thrombin / Q000378:metabolism; D014661:Vasoconstriction
D016232:Endothelins; D000990:Antithrombin III; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D013917:Thrombin; D001663:Bilirubin