High-performance liquid chromatographic determination of morphine and its metabolites in plasma using diode-array detection.
An isocratic high-performance liquid chromatographic method has been developed for the determination of morphine, codeine, normorphine, morphine 3-glucuronide and morphine 6-glucuronide in plasma using a diol column and diode-array detection. Samples were extracted using solid-phase extraction with recoveries in excess of 90%. The limit of determination was 1 ng/ml for morphine, codeine and morphine 3-glucuronide, and 10 ng/ml for normorphine and morphine 6-glucuronide. Inter- and intra-day precision were better than 10%.
Wielbo
D
D
Department of Pediatrics, University of Illinois, Chicago 60612.
Bhat
R
R
Chari
G
G
Vidyasagar
D
D
Tebbett
I R
IR
Gulati
A
A
D002851:Chromatography, High Pressure Liquid; D003061:Codeine / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009020:Morphine / Q000097:blood*; D009022:Morphine Derivatives / Q000097:blood; D012997:Solvents
D009022:Morphine Derivatives; D012997:Solvents; C035349:morphine-6-glucuronide; D009020:Morphine; C018108:morphine-3-glucuronide; D003061:Codeine; C010616:normorphine