title: Isolation and mapping of polymorphic microsatellites in cattle.
abstract: A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC-dA)n.(dG-dT)n oligonucleotide probe for the repeated nucleotide motif (CA)n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR-analysis of a panel of somatic hybrid lines.
authors.affiliation: Department of Animal Science, Swiss Federal Institute of Technology, Zurich.
authors.forename: P
authors.initials: P
authors.lastname: Steffen
authors.forename: A
authors.initials: A
authors.lastname: Eggen
authors.forename: A B
authors.initials: AB
authors.lastname: Dietz
authors.forename: J E
authors.initials: JE
authors.lastname: Womack
authors.forename: G
authors.initials: G
authors.lastname: Stranzinger
authors.forename: R
authors.initials: R
authors.lastname: Fries
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic
chemical_list: D004276:DNA, Satellite; D005819:Genetic Markers