[Changes in isoelectric points of the monoclonal antibody OC 125 during indium 111 radiolabeling].
Isoelectrofocusing analysis of the murine monoclonal antibody OC 125 (IgG1) revealed four bands at pI (6.6-7.2). After pepsin digestion, f(ab')2 pIs were increased by 0.6 pH units and two additional bands were visible. DTPA conjugation decreased pI (by up to -2 units) and increased heterogeneity (10 bands or more). With In3+ at saturation level, the conjugate was slightly modified. Autoradiography revealed pI heterogeneity of OC 125-f(ab')2-DTPA-In-111. These data show that isoelectrofocusing enables accurate monitoring of monoclonal antibody changes during In-111 labeling.
Legendre
J M
JM
UBO-UFR de Médecine, Laboratoire de Biophysique, Brest, France.
Bergot
A
A
Turzo
A
A
Morin
P P
PP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D066298:In Vitro Techniques; D007205:Indium Radioisotopes; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D051379:Mice
D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes
Modifications des points isoélectriques de l'anticorps monoclonal OC 125 lors de son radiomarquage par l'indium 111.