title: Effects of polyamine synthesis blockade on neuronal loss and astroglial reaction after transient forebrain ischemia.
abstract: Polyamines and ornithine decarboxylase, the polyamine biosynthetic enzyme, have been demonstrated to increase in the early phase of several types of brain lesion. However, their role in the pathogenesis of tissue damage is still debated. In the present paper the effects of treatments with alpha-difluoromethylornithine, a suicide inhibitor of ornithine decarboxylase, have been investigated in a model of transient forebrain ischemia. Three treatment schedules were used: alpha-difluoromethylornithine treatment was either started 3 hr before and repeated 1 hr after the insult, or started at the time of the insult and continued for 3 or 7 days after post-ischemic reperfusion. The rats were sacrificed 4 hr, 7 or 40 days after reperfusion, respectively. The acute experiment demonstrated that alpha-difluoromethylornithine can reduce the increase of glial fibrillary acid protein immunoreactivity, an early marker of astroglial reaction, in ischemic striatum. Subchronic and chronic alpha-difluoromethylornithine treatments induced a worsening of the morphological outcome of the ischemic lesion. In caudate-putamen a trend for an increase of the area of neuronal loss was present after both treatments. In the hippocampal formation, a significant increase in the severity of neuronal lesion was observed in the mildly lesioned CA3 field. In addition, other alterations of lesioned tissue were observed in alpha-difluoromethylornithine-treated animals, including increases of non-neuronal cells at 7 and especially 40 days post-lesion in striatum and CA3 hippocampal field. In conclusion, present data indicate that ornithine decarboxylase activation after ischemic lesion is a crucial factor for survival of mildly lesioned neurons and proper tissue reaction to the ischemic lesion. The experiment on acute alpha-difluoromethylornithine treatment suggests that these effects may be, at least in part, related to putrescine-induced activation of astroglial cells in the early post-lesion period.
authors.affiliation: Institute of Human Physiology, University of Modena, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Zoli
authors.forename: I
authors.initials: I
authors.lastname: Zini
authors.forename: R
authors.initials: R
authors.lastname: Grimaldi
authors.forename: G
authors.initials: G
authors.lastname: Biagini
authors.forename: L F
authors.initials: LF
authors.lastname: Agnati
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D015317:Biogenic Polyamines / Q000096:biosynthesis*; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D000518:Eflornithine / Q000494:pharmacology; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D065108:Ornithine Decarboxylase Inhibitors; D010750:Phosphoproteins; D016548:Prosencephalon / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
chemical_list: D015317:Biogenic Polyamines; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D065108:Ornithine Decarboxylase Inhibitors; D010750:Phosphoproteins; D009955:Ornithine Decarboxylase; D000518:Eflornithine