title: The human keratinocyte two-dimensional gel protein database: update 1993.
abstract: The master two-dimensional gel database of human keratinocytes currently lists 3038 cellular proteins (2127 isoelectric focusing, IEF; and 911 nonequilibrium pH gradient electrophoresis, NEPHGE) many of which correspond to post-translational modifications. 763 proteins have been identified (protein name, organelle components, etc.) and they are listed both in alphabetical order and with increasing SSP number, together with their M(r), pI, cellular localization and credit to the investigator(s) that aided in the identification. Furthermore we have listed 176 proteins that have been microsequenced so far and that are recorded in this database. We also include synthetic images depicting some interesting sets of proteins identified so far; these include components of hnRNP's, proteasomes or prosomes, ribosomes, as well as assorted organelle markers, GTP-binding proteins, calcium binding proteins, stress proteins, autoantigens, differentiation markers and psoriasis upregulated proteins. The aim of the comprehensive database is to gather, through a systematic study of keratinocytes, qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and ultimately to pinpoint signaling pathways and components affected in various skin diseases, cancer included.
authors.affiliation: Institute of Medical Biochemistry, Aarhus University.
authors.forename: J E
authors.initials: JE
authors.lastname: Celis
authors.forename: H H
authors.initials: HH
authors.lastname: Rasmussen
authors.forename: E
authors.initials: E
authors.lastname: Olsen
authors.forename: P
authors.initials: P
authors.lastname: Madsen
authors.forename: H
authors.initials: H
authors.lastname: Leffers
authors.forename: B
authors.initials: B
authors.lastname: Honoré
authors.forename: K
authors.initials: K
authors.lastname: Dejgaard
authors.forename: P
authors.initials: P
authors.lastname: Gromov
authors.forename: H J
authors.initials: HJ
authors.lastname: Hoffmann
authors.forename: M
authors.initials: M
authors.lastname: Nielsen
mesh_terms: D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D015603:Keratinocytes / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D017421:Sequence Analysis
chemical_list: D011506:Proteins