Effect of amiloride on sodium-calcium exchange activity in rat cardiac myocytes.
The effect of amiloride on Na+/Ca2+ exchange activity and adenylate energy charge in isolated rat cardiac myocytes has been studied. Amiloride inhibits Na+/Ca2+ exchange activity in rat cardiac myocytes with an IC50 of 1.76 mM when added to the external side of the cell membrane, whereas it is a weaker inhibitor when present at the cytoplasmic side. The adenylate energy charge in rat cardiac myocytes is unaffected by amiloride.
Antolini
M
M
Department of Pharmacology, University of Padova, Italy.
Trevisi
L
L
Debetto
P
P
Luciani
S
S
D000249:Adenosine Monophosphate / Q000378:metabolism; D000584:Amiloride / Q000493:pharmacokinetics / Q000494:pharmacology*; D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism; D004734:Energy Metabolism; D006321:Heart / Q000187:drug effects*; D007424:Intracellular Fluid / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism; D019831:Sodium-Calcium Exchanger
D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D000249:Adenosine Monophosphate; D000584:Amiloride