title: Interaction of various mechanical activity models in regulation of myosin heavy chain isoform expression.
abstract: The purpose of this study was to determine the effects of a novel combination of mechanical activity paradigms on the isomyosin distribution in rat hindlimb muscles. Thirty female Sprague-Dawley rats were divided into five experimental groups as follows: normal control, functional overload (OV) of the plantaris, OV in conjunction with hindlimb suspension (OV-S), and a combination of OV-S and either static standing weight-bearing activity (OV-SS) or high-incline treadmill exercise (OV-SE). OV of the plantaris resulted in significant hypertrophy and significant fast-to-slow isomyosin shifts. These changes were completely inhibited by the addition of hindlimb suspension (OV-S). Also, neither of the two weight-bearing regimes (OV-SS and OV-SE) was able to attenuate the suspension-induced atrophy. In the vastus intermedius and vastus lateralis, however, OV-SS was able to partially retard the atrophy associated with suspension. In both the plantaris and vastus intermedius, only OV-SS was able to partially reverse the slow-to-fast isomyosin transitions associated with suspension. These results suggest that the type of mechanical activity is important in determining adaptation to altered loading conditions, with OV-SS appearing more effective than OV-SE in reversing the effects of unweighting.
authors.affiliation: Department of Physiology and Biophysics, University of California, Irvine 92717.
authors.forename: G M
authors.initials: GM
authors.lastname: Diffee
authors.forename: S
authors.initials: S
authors.lastname: McCue
authors.forename: A
authors.initials: A
authors.lastname: LaRosa
authors.forename: R E
authors.initials: RE
authors.lastname: Herrick
authors.forename: K M
authors.initials: KM
authors.lastname: Baldwin
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D005260:Female; D006614:Hindlimb / Q000502:physiology; D007536:Isomerism; D008954:Models, Biological; D009132:Muscles / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D009210:Myofibrils / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure; D009218:Myosins / Q000096:biosynthesis*; D009929:Organ Size / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014893:Weightlessness / Q000009:adverse effects
chemical_list: D000251:Adenosine Triphosphatases; D009218:Myosins