New simplified molecular design for functional T cell receptor.
We have produced a chimeric single-chain T cell receptor (TcR) that combines the specific antibody recognition function and TcR/CD3 signaling properties within the same polypeptide chain. This hybrid molecule consisted of a single-chain antibody combining site that was connected over a short spacer to the transmembrane and cytoplasmic region of CD3 zeta. When expressed on TcR- or TcR+ T cell hybridomas it could mediate recognition of relevant target cells and subsequent production of lymphokines; i.e. it could functionally replace the TcR/CD3 complex. Therefore, the single-chain TcR model presented here represents an interesting and useful means for the creation of T cells with new specificities.
Brocker
T
T
Basel Institute for Immunology, Switzerland.
Peter
A
A
Traunecker
A
A
Karjalainen
K
K
D001483:Base Sequence; D001666:Binding Sites, Antibody; D017252:CD3 Complex / Q000737:chemistry*; D015195:Drug Design; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry*; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D014162:Transfection; D014407:Tumor Cells, Cultured
D017252:CD3 Complex; D007140:Immunoglobulin Fab Fragments; D009841:Oligonucleotides; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins