title: The active adenovirus protease is the intact L3 23K protein.
abstract: The L3 23K protein was isolated from adenovirus type 2 and shown to cleave purified substrates, confirming that this protein is the adenovirus protease. Separate antisera, prepared against the amino- and carboxy-terminal regions of the 23K protein react with active protease, demonstrating that, contrary to previous reports, zymogen activation is not involved in the regulation of this enzyme. Molecular exclusion chromatography indicated that the protease is active as a monomer. Purified protease was shown to be inhibited by Zn2+ and Cu2+ and by some, but not all, recognized cysteine protease inhibitors, indicating participation of a thiol group and providing additional support to the suggestion that regulation of the enzyme involves a form of thiol-disulphide interchange.
authors.affiliation: Division of Cell and Molecular Biology, School of Biological and Medical Sciences, University of St Andrews, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Webster
authors.forename: G
authors.initials: G
authors.lastname: Kemp
mesh_terms: D000260:Adenoviruses, Human / Q000201:enzymology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002413:Cations, Divalent / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011480:Protease Inhibitors / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D014764:Viral Proteins
chemical_list: D002413:Cations, Divalent; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D009842:Oligopeptides; D010446:Peptide Fragments; D011480:Protease Inhibitors; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D005982:Glutathione Transferase; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C082284:L3 23K protein, adenovirus