title: A simple, modified technique for direct chromosome preparation from chorionic villi.
abstract: Chorionic villus sampling (CVS) has proved to be an elegant and safe technique for prenatal diagnosis of genetic diseases during the first trimester of pregnancy. Despite a number of obvious advantages, the wide application of direct cytogenetic analysis of CVS has been hampered by serious technical problems involving large numbers of incomplete metaphases and poor chromosome morphology. A chromosome preparation technique direct from CVS has been devised which involves modifications in the hypotonic and dissociation stages. It yielded satisfactory results in fifteen samples within 24 h of sampling. It is suggested that this simple, modified technique can provide complete chromosomal analysis within a short time for prenatal diagnosis of genetic diseases, and may be used as a routine method in cytogenetic laboratories.
authors.affiliation: Department of Genetics, Barkatullah University, Bhopal, India.
authors.forename: B
authors.initials: B
authors.lastname: Jain
authors.forename: L K
authors.initials: LK
authors.lastname: Sengupta
mesh_terms: D000328:Adult; D015193:Chorionic Villi Sampling / Q000379:methods*; D005260:Female; D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D016591:Histocytological Preparation Techniques; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*