title: A class-A beta-lactamase from Pseudomonas stutzeri that is highly active against monobactams and cefotaxime.
abstract: A beta-lactamase produced by Pseudomonas stutzeri was purified to protein homogeneity, and its physicochemical and catalytic properties were determined. Its profile was unusual since, in addition to penicillins, the enzyme hydrolysed second- and third-generation 'beta-lactamase-stable' cephalosporins and monobactams with similar efficiencies. On the basis of the characteristics of the interaction with beta-iodopenicillanic acid, the enzyme could be classified as a class-A beta-lactamase. However, when compared with most class-A beta-lactamases, it exhibited significantly lower kcat./Km values for the compounds usually considered to be the best substrates of these enzymes.
authors.affiliation: Università degli studi dell'Aquila, Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Franceschini
authors.forename: M
authors.initials: M
authors.lastname: Galleni
authors.forename: J M
authors.initials: JM
authors.lastname: Frère
authors.forename: A
authors.initials: A
authors.lastname: Oratore
authors.forename: G
authors.initials: G
authors.lastname: Amicosante
mesh_terms: D002439:Cefotaxime / Q000378:metabolism*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008997:Monobactams / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D008997:Monobactams; D001618:beta-Lactamases; D002439:Cefotaxime