title: Differential expression of immediate-early proteins in non-nerve cells after focal brain injury.
abstract: We investigated the expression of the immediate-early proteins (IEPs, Fos, Fos B, Jun, Jun B, Jun D, Krox 20 and Krox 24) in non-nerve cells in rat brain after mechanical brain injury. Injury produced by infusion of 5 microliters of saline into the hippocampus produced a time-dependent expression of Fos, Jun and Krox 24, but not Fos B, Krox 20 or in non-nerve cells around the wound margin, in cells lining the lateral and third ventricles and in cells in the pial surfaces of the brain. Jun B and D were weakly induced in non-nerve cells 1-4 hr after brain injury. This differential expression of IEPs in non-nerve cells contrasted with neurons which expressed all IEPs measured. Thus, brain injury is associated with a differential expression of IEPs in non-nerve cells around the wound. The functional implications of this IEP expression after brain injury are presently unclear, but may be related to cellular proliferation after brain injury.
authors.affiliation: Department of Pharmacology, School of Medicine, University of Auckland, New Zealand.
authors.forename: M
authors.initials: M
authors.lastname: Dragunow
authors.forename: P
authors.initials: P
authors.lastname: Hughes
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D001930:Brain Injuries / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D051766:Early Growth Response Protein 1; D051767:Early Growth Response Protein 2; D016762:Genes, fos; D016758:Genes, jun; D017874:Immediate-Early Proteins; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; D051767:Early Growth Response Protein 2; C486110:Egr1 protein, rat; C497328:Egr2 protein, rat; D017874:Immediate-Early Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors