Increased detection of human immunodeficiency virus antigenemia after dissociation of immune complexes at low pH.
This study compared the number of patients with detectable human immunodeficiency virus (HIV) antigenemia after immune complex (IC) dissociation by established methods using either 0.5 NCl or 1.5 M glycine buffer. Without IC dissociation, HIV antigen was detected in 43% of patients. After dissociation, the HCl method detected only an additional 7% of patients (P = 0.09), while the glycine method detected an additional 34% (P < 0.001). However, care must be taken in setting the threshold of the standards, and confirmatory neutralization assays should be performed to ensure specificity of HIV antigen enzyme immunoassay after IC dissociation.
Pokriefka
R A
RA
Department of Internal Medicine, Henry Ford Hospital, Detroit, Michigan 48202.
Manzor
O
O
Markowitz
N P
NP
Saravolatz
L D
LD
Kvale
P
P
Donovan
R M
RM
D000936:Antigen-Antibody Complex / Q000097:blood* / Q000302:isolation & purification; D002021:Buffers; D005069:Evaluation Studies as Topic; D005998:Glycine; D016655:HIV Core Protein p24 / Q000097:blood* / Q000302:isolation & purification; D015658:HIV Infections / Q000382:microbiology*; D006801:Humans; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D000936:Antigen-Antibody Complex; D002021:Buffers; D016655:HIV Core Protein p24; D006851:Hydrochloric Acid; D005998:Glycine