Injury induced expression of TGF-beta 1 mRNA is enhanced by exogenously applied TGF-beta S.
We have analysed and compared, by in situ hybridisation, the effects of exogenously applied TGF-beta s on expression of endogenous TGF-beta mRNAs in partial thickness thermal wounds in old and young mice. Although injury induced the expression of TGF-beta 1 mRNA in the epidermis and dermis at the wound margins, expression of TGF-beta 2- or TGF-beta 3-mRNA was not detected. Biopsies taken 24 hours following injury revealed a focally clustered distribution of TGF-beta 1 hybridisation signals in the dermis, the number of positive cells and expression levels being reduced in old mice. Topical application of all three TGF-beta isoforms enhanced TGF-beta 1 mRNA expression in the dermis of old and young mice. In biopsies taken three days following injury, TGF-beta 1 hybridisation signals were most prominent in the regenerating epidermis although at this timepoint differences in expression levels between treated and non-treated animals were less pronounced.
Schmid
P
P
CIBA-GEIGY Ltd, Pharma Division, Basel.
Kunz
S
S
Cerletti
N
N
McMaster
G
G
Cox
D
D
D000818:Animals; D001706:Biopsy; D002056:Burns / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D012333:RNA, Messenger; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta