title: Fatty acid uptake in Escherichia coli: regulation by recruitment of fatty acyl-CoA synthetase to the plasma membrane.
abstract: Fatty acid uptake in Escherichia coli has been shown to be inhibited by starvation and to be reversed by a short preincubation of the starved cells with D- or L-lactate, succinate, and acetate; these effects on oleate uptake were due to regulation of the rate-limiting step which involves fatty acyl-CoA synthetase. Investigation into the mechanism of regulation of fatty acyl-CoA synthetase showed that D-lactate did not affect the activity of the enzyme directly. Fatty acyl-CoA synthetase was found to be activated by about 20-fold by Triton X-100 and by another 4-fold by the addition of bacterial membranes. D-Lactate treatment was shown to result in coisolation of fatty acyl-CoA synthetase with the plasma membrane; these results are consistent with the interpretation that recruitment of the enzyme to the plasma membrane by D-lactate results in its activation and consequently in the increased level of fatty acid uptake.
authors.affiliation: Department of Biochemistry, McMaster University, Hamilton, Ont., Canada.
authors.forename: D
authors.initials: D
authors.lastname: Mangroo
authors.forename: G E
authors.initials: GE
authors.lastname: Gerber
mesh_terms: D000085:Acetates / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002451:Cell Compartmentation / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D003066:Coenzyme A Ligases / Q000187:drug effects / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D007773:Lactates / Q000494:pharmacology; D017830:Octoxynol; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid
chemical_list: D000085:Acetates; D007773:Lactates; D009829:Oleic Acids; D013386:Succinates; D019301:Oleic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; D019802:Succinic Acid; D003066:Coenzyme A Ligases