A comparative study of the in vitro and in vivo formation of lanosterol derivatives from acetate by different chick tissues.
The optimal conditions for the synthesis of lanosterol derivatives, previously demonstrated as inhibitors of cholesterogenesis, have been studied in 14-day-old chicks using acetate as precursor. In experiments carried out in vitro, the relative percentage of lanosterol derivatives in the total nonsaponifiable fraction was practically similar in each tissue at any incubation time considered (15-120 min). This percentage was higher in kidney and duodenal mucosa than in liver. The percentage of lanosterol derivatives was constant at any acetate concentration assayed (1-12 mM) in liver and duodenal mucosa, while in kidney this percentage increased at high acetate concentration (8-12 mM). In experiments carried out in vivo, the relative percentage of lanosterol derivatives was lower than that found in each tissue in the in vitro conditions. This behaviour was opposed to the acetate incorporation into cholesterol in the same experimental conditions. No significant differences were observed in the in vivo acetate incorporation into lanosterol derivatives by chick brain at different times of day. However, in duodenal mucosa and kidney maximal values were found during the dark period, on the contrary to that previously observed in the percentage of cholesterol. Our results demonstrated that kidney and duodenal mucosa were the chick tissues in which the higher percentage of lanosterol derivatives was accumulated.
Arce
V
V
Department of Biochemistry and Molecular Biology, University of Granada, Spain.
Linares
A
A
Aguilera
J A
JA
Garcia-Peregrin
E
E
D000085:Acetates / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D004386:Duodenum / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D007810:Lanosterol / Q000031:analogs & derivatives / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D014018:Tissue Distribution
D000085:Acetates; D007810:Lanosterol