Irrigation with waste water: parasitological analysis of soil.
In third world countries, the agricultural use of treated wastewater represents an interesting solution. A recent report of W.H.O. shows the importance of strict parasitological criteria for such reuse. The aim of this paper is to study the conditions under which Ascaris eggs have been recovered from artificially contaminated soils (sandy, clay or loamy soil, mould garden). The eggs elution has been carried out from soil particles using various solutions (detergents, distilled water, formaldehyde, sodium hydroxide, sodium hypochlorite). The recovery percentage analysis showed the superiority of the sodium hypochlorite solution titrating 10 chlorometric degrees, whatever the soil type. The eggs concentration in the eluates has been carried out through the flotation technique by testing various reagents with densities ranging from 1.16 to 1.44. The zinc sulphate solution at 50%, 55% and 66% prove to be flotation agents that are well adapted to this sampling type. By carrying out the elution with a sodium hypochlorite solution titrating 10 chlorometric degrees and the concentration by flotation with a zinc sulphate solution at 55% the recovery percentages, which are independent of the parasitic load, vary from 66 to 78%.
Gaspard
P
P
Département de Microbiologie, WHO Collaborating Centre, Faculté de Pharmacie, Nancy, France.
Schwartzbrod
J
J
D000383:Agriculture / Q000379:methods*; D000818:Animals; D001200:Ascaris / Q000302:isolation & purification*; D003906:Developing Countries; D010270:Parasite Egg Count; D012987:Soil; D014865:Waste Disposal, Fluid; D014944:World Health Organization
D012987:Soil