title: The CRE-binding factor ATF-2 facilitates the occupation of the CCAAT box in the fibronectin gene promoter.
abstract: The cAMP response element (CRE) and the CCAAT box of the fibronectin gene promoter are separated by only twenty base pairs. A specific factor that binds the CRE interacts cooperatively with the protein which binds to the adjacent CCAAT box, stimulating transcription [1992, J. Biol. Chem. 267, 12767-12774]. Here we show that the CRE factor is an heterodimer between a 43 kDa and the '73 kDa' CRE-binding proteins and we identify the latter as ATF-2 (also named CRE-BPI), a protein implicated in recruiting transcriptional activators to promoters, able to form heterodimers with Jun and for which a sequence-deduced MW of 55 kDa had been previously reported.
authors.affiliation: Instituto de Investigaciones en Ingeniería Genética y Biología, Molecular (INGEBI) CONICET, Universidad de Buenos Aires, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Srebrow
authors.forename: A F
authors.initials: AF
authors.lastname: Muro
authors.forename: S
authors.initials: S
authors.lastname: Werbajh
authors.forename: P A
authors.initials: PA
authors.lastname: Sharp
authors.forename: A R
authors.initials: AR
authors.lastname: Kornblihtt
mesh_terms: D051696:Activating Transcription Factors; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics*; D005353:Fibronectins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D051696:Activating Transcription Factors; D001798:Blood Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D005353:Fibronectins; D009687:Nuclear Proteins; D014157:Transcription Factors