Tumor necrosis factor is a mediator of phospholipase release during bacteremia in baboons.
Phospholipase A (PLA) activity is elevated in plasma after polytrauma and in sepsis. During both situations, tumor necrosis factor (TNF) and free fatty acids (FFAs) are also increased. To determine whether there is a relationship between the changes in PLA and in TNF that occur during sepsis, we used a primate model of bacteremia. Live Escherichia coli (5 x 10(8) colony-forming units/kg) was injected into anesthetized baboons (n = 10). Four of these animals were pretreated with an anti-TNF antibody (anti-TNF Ab, 15 mg/kg). After the administration of bacteria (2 h), the plasma TNF was 10.2 +/- 3.2 ng/ml. PLA and FFA reached their maximum values of 191 +/- 14 U/l and 1.5 +/- 0.3 mM at 10 and 24 h after the injection of bacteria, respectively. In the animals treated with anti-TNF Ab, plasma TNF was undetectable; the highest values recorded for PLA and FFA were 71 +/- 23 U/l and 0.7 +/- 0.3 mM, respectively. Thus TNF appears to be an important mediator of PLA release during sepsis.
Redl
H
H
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria.
Schlag
G
G
Schiesser
A
A
Davies
J
J
D000818:Animals; D000906:Antibodies / Q000276:immunology; D016470:Bacteremia / Q000201:enzymology / Q000503:physiopathology*; D004927:Escherichia coli Infections / Q000201:enzymology* / Q000503:physiopathology*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D008297:Male; D010215:Papio; D010741:Phospholipases A / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000502:physiology*
D000906:Antibodies; D005230:Fatty Acids, Nonesterified; D014409:Tumor Necrosis Factor-alpha; D010741:Phospholipases A