title: Caldesmon and a 20-kDa actin-binding fragment of caldesmon inhibit tension development in skinned gizzard muscle fiber bundles.
abstract: Caldesmon is known to inhibit actin-activated myosin ATPase activity in solution, to inhibit force production when added to skeletal muscle fibers, and to alter actin movement in the in vitro cell motility assay. It is less clear that caldesmon can inhibit contraction in smooth muscle cells in which caldesmon is abundant. We now show that caldesmon and its 20-kDa actin-binding fragment are able to inhibit force in chemically skinned gizzard fiber bundles, which are activated by a constitutively active myosin light-chain kinase in the presence and absence of okadaic acid. This inhibitory effect is reversed by high concentrations of Ca2+ and calmodulin. Therefore, caldesmon may act by increasing the level of myosin phosphorylation required to obtain full activation. Our results also suggest that caldesmon does not act to maintain force in smooth muscle by cross-linking myosin with actin since competition of binding of caldesmon with myosin does not cause a reduction in tension.
authors.affiliation: II, Physiologisches Institut, Universität Heidelberg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Pfitzer
authors.forename: C
authors.initials: C
authors.lastname: Zeugner
authors.forename: M
authors.initials: M
authors.lastname: Troschka
authors.forename: J M
authors.initials: JM
authors.lastname: Chalovich
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002148:Calmodulin-Binding Proteins / Q000378:metabolism / Q000494:pharmacology*; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D009218:Myosins / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D010766:Phosphorylation
chemical_list: D000199:Actins; D002148:Calmodulin-Binding Proteins; D010446:Peptide Fragments; D009218:Myosins; D002118:Calcium