title: Tumor cell-derived collagenase-stimulatory factor increases expression of interstitial collagenase, stromelysin, and 72-kDa gelatinase.
abstract: The tumor cell-derived collagenase-stimulatory factor (TCSF) was previously purified from human lung carcinoma cells (S. M. Ellis, K. Nabeshima, and C. Biswas, Cancer Res., 49: 3385-3391, 1989). This protein is present on the surface of several types of human tumor cells in vitro and in vivo and stimulates production of interstitial collagenase in human fibroblasts. In this study it is shown that TCSF stimulates expression in human fibroblasts of mRNA for stromelysin 1 and 72-kDa gelatinase/type IV collagenase, as well as for interstitial collagenase. Measurement of enzyme protein by immunoassay showed that the amounts of interstitial collagenase and stromelysin 1 were increased in TCSF-treated fibroblasts; gelatin zymography indicated that there was an increase in the 72-kDa gelatinase. These results indicate that tumor cell interaction with fibroblasts via TCSF could lead to increased degradation of interstitial or basement membrane matrix components and thus to enhanced tumor cell invasion.
authors.affiliation: Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111.
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: R
authors.initials: R
authors.lastname: DeCastro
authors.forename: S
authors.initials: S
authors.lastname: Zucker
authors.forename: C
authors.initials: C
authors.lastname: Biswas
mesh_terms: D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D051926:Basigin; D014408:Biomarkers, Tumor; D015152:Blotting, Northern; D017364:Collagenases / Q000235:genetics*; D015342:DNA Probes; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics*; D020781:Matrix Metalloproteinase 1; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D008666:Metalloendopeptidases / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013268:Stimulation, Chemical; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; C497886:BSG protein, human; D014408:Biomarkers, Tumor; C497887:Bsg protein, mouse; D015342:DNA Probes; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D051926:Basigin; D014568:Urokinase-Type Plasminogen Activator; D017364:Collagenases; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1