Wheat germ lectin affinity electrophoresis of serum alkaline phosphatase with commercially available agarose gels.
We have modified a commercially available procedure involving precast agarose gels (Paragon Isopal System) to measure alkaline phosphatase (EC 3.1.3.1) isoenzymes. Including wheat germ lectin in the equilibration buffer improved the resolution of the bone and liver isoenzymes. Unlike previously described wheat germ lectin affinity electrophoresis methods, the procedure measures bone, liver, and biliary isoenzymes in a single step. There was good correlation between the affinity electrophoresis and the neuraminidase preincubation methods for the measurement of bone (r = 0.958) and liver (r = 0.962) alkaline phosphatase isoenzymes. However, the affinity electrophoresis method also quantified minor isoenzyme fractions that were poorly resolved by the neuraminidase method. The method is technically simple, reproducible, and capable of rapid handling of large workloads.
Mattiazzo
M
M
Department of Biochemistry, Repatriation Hospital, Daw Park, Australia.
Ramasamy
I
I
D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012044:Regression Analysis; D014909:Wheat Germ Agglutinins
D007527:Isoenzymes; D014909:Wheat Germ Agglutinins; D000469:Alkaline Phosphatase