FcR gamma chain deletion results in pleiotrophic effector cell defects.
The gamma subunit of immunoglobulin Fc receptors is an essential component of the high-affinity receptor for IgG (Fc gamma RIII) and is associated with the high-affinity receptor for IgG (Fc gamma RI) and the T cell receptor-CD3 complex. It is required for both receptor assembly and signal transduction. Targeted disruption of this subunit results in immunocompromised mice. Activated macrophages from gamma chain-deficient mice unexpectedly lack the ability to phagocytose antibody-coated particles, despite normal binding. Defects in NK cell-mediated antibody-dependent cytotoxicity and mast cell-mediated allergic responses are evident in these animals, establishing the indispensable role of FcRs in these responses. However, loss of gamma chain does not appear to perturb T cell development, since both thymic and peripheral T cell populations appear normal. These mice thus represent an important tool for evaluating the role of these receptors in humoral and cellular immune responses.
Takai
T
T
DeWitt Wallace Research Laboratory, Sloan-Kettering Institute, New York, New York 10021.
Li
M
M
Sylvestre
D
D
Clynes
R
R
Ravetch
J V
JV
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D002678:Chimera; D003602:Cytotoxicity, Immunologic; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D005260:Female; D005434:Flow Cytometry; D017353:Gene Deletion; D005838:Genotype; D015847:Interleukin-4 / Q000096:biosynthesis; D046911:Macromolecular Substances; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010323:Passive Cutaneous Anaphylaxis; D010587:Phagocytosis; D016133:Polymerase Chain Reaction; D015230:Prostaglandin D2 / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D017455:Receptors, IgE / Q000235:genetics / Q000502:physiology*; D017452:Receptors, IgG / Q000235:genetics / Q000502:physiology*; D013154:Spleen / Q000276:immunology; D013234:Stem Cells; D013601:T-Lymphocytes / Q000276:immunology*
D017931:DNA Primers; D046911:Macromolecular Substances; D012333:RNA, Messenger; D017455:Receptors, IgE; D017452:Receptors, IgG; D015847:Interleukin-4; D004247:DNA; D015230:Prostaglandin D2