title: IFN-gamma is directly cytostatic to the extracellular form (promastigote) of Leishmania donovani.
abstract: We investigated the effect of various human cytokines on the in vitro growth of Leishmania donovani (L. donovani) promastigotes. rhIFN-alpha, rhIFN-beta, rhIL-1, rhIL-2, rhIL-3, rhIL-4, rhIL-6 and rhM-CSF had no effect. By contrast, rhIFN-gamma was directly growth inhibitory to L. donovani promastigotes in a dose-dependent manner. Anti-IFN-gamma antibody, but neither anti-IFN-alpha nor anti-IFN-beta antibody, abolished this promastigote growth inhibitory effect of rhIFN-gamma. L. donovani promastigotes were not lysed by rhIFN-gamma as determined by 51Cr-release assay. These data indicate that rhIFN-gamma is cytostatic, not cytotoxic, to L. donovani promastigotes.
authors.affiliation: Indian Institute of Chemical Biology, Calcutta.
authors.forename: S
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: P P
authors.initials: PP
authors.lastname: Manna
authors.forename: S
authors.initials: S
authors.lastname: Bandyopadhyay
mesh_terms: D000818:Animals; D002860:Chromium Radioisotopes; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007893:Leishmania donovani / Q000187:drug effects* / Q000254:growth & development*; D008262:Macrophage Activation / Q000187:drug effects; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D002860:Chromium Radioisotopes; D007370:Interferon Type I; D007375:Interleukin-1; D007376:Interleukin-2; D007377:Interleukin-3; D015850:Interleukin-6; D011994:Recombinant Proteins; D015847:Interleukin-4; D016899:Interferon-beta; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma