Stripe-specific regulation of pair-rule genes by hopscotch, a putative Jak family tyrosine kinase in Drosophila.
We describe the characterization of the Drosophila gene, hopscotch (hop), which is required maternally for the establishment of the normal array of embryonic segments. In hop embryos, although expression of the gap genes appears normal, there are defects in the expression patterns of the pair-rule genes even-skipped, runt, and fushi tarazu, as well as the segment-polarity genes engrailed and wingless. We demonstrate that the effect of hop on the expression of these genes is stripe-specific. The hop gene encodes a putative nonreceptor tyrosine kinase of the Janus kinase family, based on an internal duplication of the catalytic domain. We present a model in which the Hop tyrosine kinase is involved in the control of pair-rule gene transcription in a stripe-specific manner. Our results provide the first evidence for stripe-specific regulation of pair-rule genes by a tyrosine kinase.
Binari
R
R
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Perrimon
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology* / Q000201:enzymology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect; D005804:Genes, Lethal; D053614:Janus Kinase 2; D053612:Janus Kinases; D007814:Larva; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D011518:Proto-Oncogene Proteins; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics; D014960:X Chromosome
D029721:Drosophila Proteins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases; D053614:Janus Kinase 2; D053612:Janus Kinases; C085845:hop protein, Drosophila