Metabolic activation of aflatoxin B1 by human placental microsomes.
The metabolic activation of aflatoxin B1 by human placental microsomes was studied. Aflatoxin B1 showed relatively high mutagenic activity in Ames test when incubated with human placental microsomes. Addition of alpha-naphthoflavone or aminoglutethimide, known inhibitors of cytochrome P450 1A and P450 19, respectively, into the test system partially inhibited the mutagen-producing activity. It was suggested that the activation of aflatoxin B1 in human placental microsomes is mediated by at least these two forms of cytochrome P450.
Sawada
M
M
Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Kitamura
R
R
Norose
T
T
Kitada
M
M
Itahashi
K
K
Kamataki
T
T
D016604:Aflatoxin B1 / Q000378:metabolism* / Q000633:toxicity; D000616:Aminoglutethimide / Q000494:pharmacology; D001571:Benzoflavones / Q000494:pharmacology; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D010920:Placenta / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D011247:Pregnancy; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D009153:Mutagens; D000616:Aminoglutethimide; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1