Enantioselective assay of warfarin in blood plasma by liquid chromatography on Chiralcel OC.
A stereoselective method for the two enantiomers of warfarin has been developed. Warfarin is extracted into dichloromethane-hexane from blood plasma. After concentration by evaporation, the enantiomers are resolved by liquid chromatography on Chiralcel OC employing a non-polar mobile phase. By fluorometric detection, concentrations in plasma down to 80 nmol/l (25 ng/ml) can be determined with a coefficient of variation of less than 15%. At the 200 nmol/l level, a coefficient of variation of ca. 4% was found.
Andersen
C
C
Astra Hässle AB, Mölndal, Sweden.
Balmér
K
K
Lagerstróm
P O
PO
D002219:Carbamates; D002482:Cellulose; D002853:Chromatography, Liquid; D006801:Humans; D048448:Phenylcarbamates; D010949:Plasma / Q000737:chemistry; D012015:Reference Standards; D012997:Solvents; D013237:Stereoisomerism; D014859:Warfarin / Q000097:blood*
D002219:Carbamates; D048448:Phenylcarbamates; D012997:Solvents; C079151:cellulose trisphenylcarbamate; D014859:Warfarin; D002482:Cellulose