title: Protein-conjugated synthetic di- and trisaccharides of pneumococcal type 17F exhibit a different immunogenicity and antigenicity than tetrasaccharide.
abstract: Overlapping synthetic disaccharide, trisaccharide and tetrasaccharide, derived from pneumococcal polysaccharide type 17F (PS17F), were coupled to keyhole limpet haemocyanin (KLH). The conjugates were tested in mice. The disaccharide-KLH and especially trisaccharide-KLH, in combination with Quil A, induced high titres of high-avidity anti-PS17F IgG. Both conjugates protected mice against challenge with Streptococcus pneumoniae 17F. Tetrasaccharide-KLH, although able to elicit anti-tetrasaccharide antibodies, induced a minimal non-protective anti-PS17F IgG response of low avidity. The tetrasaccharide-KLH conjugate, in contrast to the other conjugates, failed to bind rabbit anti-PS17F IgG.
authors.affiliation: Eijkman-Winkler Laboratory of Medical Microbiology, Utrecht University, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Alonso de Velasco
authors.forename: A F
authors.initials: AF
authors.lastname: Verheul
authors.forename: G H
authors.initials: GH
authors.lastname: Veeneman
authors.forename: L J
authors.initials: LJ
authors.lastname: Gomes
authors.forename: J H
authors.initials: JH
authors.lastname: van Boom
authors.forename: J
authors.initials: J
authors.lastname: Verhoef
authors.forename: H
authors.initials: H
authors.lastname: Snippe
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D002240:Carbohydrate Sequence; D005260:Female; D006433:Hemocyanins / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D000922:Immunotoxins / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology* / Q000378:metabolism; D011008:Pneumococcal Infections / Q000517:prevention & control; D011135:Polysaccharides, Bacterial / Q000276:immunology* / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D000922:Immunotoxins; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin