DNA replication of parvovirus B 19 in a human erythroid leukemia cell line (JK-1) in vitro.
A major limitation of studies on the parvovirus B 19, a causative agent of transient aplastic crisis, has been the absence of appropriate cell lines permissive for the virus. In the present study, a human erythroid leukemia cell line (JK-1) was shown to support B 19 virus DNA replication in vitro. Forty-eight hours after virus inoculation of JK-1 liquid cell cultures, the average number of B 19 genome copies was estimated at 3,000 per cell by DNA dot blot analysis. The addition of erythropoietin increased B 19 copy number to 10,000 per cell. The presence of replicative forms of the B 19 virus genome was genome was demonstrated by Southern blot analysis. Although persistent infection of B 19 virus was not observed in JK-1 cells, this culture system will be of value in elucidating the molecular basis of the erythroid specificity of parvovirus B 19.
Takahashi
T
T
Department of Hematology-Oncology, Institute of Medical Science, University of Tokyo, Japan.
Ozawa
K
K
Takahashi
K
K
Okuno
Y
Y
Takahashi
T
T
Muto
Y
Y
Takaku
F
F
Asano
S
S
D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D016732:Parvovirus B19, Human / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004279:DNA, Viral