Modulation by ascorbic acid of the cutaneous and hepatic biochemical effects induced by topically applied benzanthrone in mice.
Modulation of biochemical markers by ascorbic acid was investigated in mice to which benzanthrone (BA) was applied topically (150 nmol/mouse) twice a week for 34 wk. After BA exposure without ascorbic acid, in the skin there were significant decreases in the activities of aryl hydrocarbon hydroxylase (AHH; 38% decrease relative to controls) and ethoxyresorufin-O-deethylase (EROD; 39%), and enhancement of the activities of quinone reductase (41% increase), tyrosinase (82%) and histidine decarboxylase (HDC; 190%). BA exposure also caused significant inhibition of hepatic AHH, EROD and glutathione-S-transferase activities, with concomitant increases in the activities of histidase (52%) and HDC (58%). Ascorbic acid given orally (5 mg/mouse) or topically (1 mg/mouse) twice weekly for 34 wk to BA-treated mice resulted in substantial protection against the effects of BA on these enzyme markers in both the skin and the liver. These results suggest that ascorbic acid could be useful in preventing the biochemical and toxicological manifestations caused by BA in laboratory animals.
Dwivedi
N
N
Dyes and Food Adulterant Toxicology Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
Das
M
M
Joshi
A
A
Singh
G B
GB
Khanna
S K
SK
D000287:Administration, Topical; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000187:drug effects / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D001551:Benz(a)Anthracenes / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D006638:Histidine Ammonia-Lyase / Q000187:drug effects / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D014442:Monophenol Monooxygenase / Q000187:drug effects / Q000378:metabolism; D010088:Oxidoreductases / Q000187:drug effects / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000201:enzymology
D001551:Benz(a)Anthracenes; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1; D014442:Monophenol Monooxygenase; D005982:Glutathione Transferase; D006640:Histidine Decarboxylase; D006638:Histidine Ammonia-Lyase; C012768:benzanthrone; D001205:Ascorbic Acid