title: The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.
abstract: Two temperature-sensitive mutations, ams-1 and rne-3071, in the ams (altered mRNA stability) gene have been used extensively to investigate the processing and decay of RNA in Escherichia coli. We have sequenced these temperature-sensitive alleles and found that the mutations are separated by only 6 nucleotides and cause conservative amino acid substitutions next to a possible nucleotide-binding site within the N-terminal domain of the Ams protein. Computer analysis revealed that the region altered by the mutations has extensive sequence similarity to a predicted gene product from the mre (murein pathway cluster e) locus of E. coli, which has been implicated previously in determining bacterial cell shape.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, California 94305.
authors.forename: K J
authors.initials: KJ
authors.lastname: McDowall
authors.forename: R G
authors.initials: RG
authors.lastname: Hernandez
authors.forename: S
authors.initials: S
authors.lastname: Lin-Chao
authors.forename: S N
authors.initials: SN
authors.lastname: Cohen
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002373:Cyclic AMP Receptor Protein; D004722:Endoribonucleases; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D011953:Receptors, Cyclic AMP / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins; D012333:RNA, Messenger; D011953:Receptors, Cyclic AMP; D000255:Adenosine Triphosphate; D004722:Endoribonucleases; C081905:rng protein, E coli; C022537:ribonuclease E