[Relation between pigment gallstone formation and the ability of bile to scavenge superoxide radicals].
Operative stenosis of common bile duct (CBD) (S group) induced pigment gallstones in 14 of 16 guinea pigs one week after operation. If Vit E and Vit C were given intraperitoneally 10 mg/kg daily from 3 days before to one week after the operation (S+V group), the gallstone incidence decreased to 5/14 (P < 0.01). In comparison with control group (C), unconjugated bilirubin (UCB) and Ca2+ concentration in gallbladder bile of S group increased significantly (P < 0.05 and P < 0.01 respectively), while the scavenge rate (SR) of superoxide radical (O2-.) in bile decreased significantly (P < 0.05). Comparing S+V group with S group showed that the differences of UCB and Ca2+ concentration were not significant (P < 0.05 both); only the SR rose significantly (P < 0.05) and reached the level of C group. It is suggested that besides the increase of UCB and Ca2+ concentration, the participation of oxygen radicals may be needed in formation of pigment gallstones induced by bile duct obstruction.
Lin
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Department of Surgery, Third Hospital, Bejing Medical University.
D000818:Animals; D001205:Ascorbic Acid / Q000627:therapeutic use; D001646:Bile / Q000502:physiology*; D001654:Bile Pigments; D001663:Bilirubin; D002769:Cholelithiasis / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control; D002779:Cholestasis / Q000150:complications; D016166:Free Radical Scavengers; D006168:Guinea Pigs; D008297:Male; D010100:Oxygen / Q000378:metabolism; D014810:Vitamin E / Q000627:therapeutic use
D001654:Bile Pigments; D016166:Free Radical Scavengers; D014810:Vitamin E; D001205:Ascorbic Acid; D001663:Bilirubin; D010100:Oxygen