title: Defects of the rat premaxilla as a model of alveolar clefts for testing bone-inductive agents.
abstract: Critical-sized defects were made in the premaxillary bone of Wistar male rats using a surgical trephine and a low-speed dental engine as a model of the maxillary alveolar cleft for testing bone-inductive agents. The defects were treated with either 7 mg of demineralized bone matrix (DBM) or were left nongrafted. The nongrafted group healed mainly with fibrous connective tissue, with a small amount of bone formation at the periphery. There was no significant change in alkaline phosphatase activity and 45Ca incorporation. The DBM-grafted group produced new bone with osseous bridging in the defect by day 35. Alkaline phosphatase activity increased significantly from day 10, reaching a maximum on day 14, and 45Ca incorporation increased on day 14. These results indicate that this nonhealing bony wound of the premaxilla in rats may be useful as a model for studying the effect of bone-inductive agents on the healing of alveolar clefts.
authors.affiliation: Department of Orthodontics, Osaka University, Faculty of Dentistry, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takano-Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Kawakami
authors.forename: M
authors.initials: M
authors.lastname: Sakuda
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000539:Alveolar Process / Q000503:physiopathology / Q000601:surgery*; D000543:Alveoloplasty; D000704:Analysis of Variance; D000818:Animals; D001857:Bone Matrix / Q000637:transplantation*; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000379:methods*; D002113:Calcification, Physiologic; D002972:Cleft Palate / Q000628:therapy*; D004195:Disease Models, Animal; D006133:Growth Substances; D008297:Male; D008422:Materials Testing; D008437:Maxilla / Q000503:physiopathology / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006133:Growth Substances; D000469:Alkaline Phosphatase