A role for the fyn oncogene in metastasis of methylcholanthrene-induced fibrosarcoma A cells.
Expression of various oncogenes (ras, myc, erbB2, src, fyn, yes and sis) in a high-metastatic clone (MH-02) derived from a murine methylcholanthrene-induced fibrosarcoma A (Meth A) was compared with those of its parent clone (ML-01) by Northern blot analysis. Two oncogenes, fyn, belonging to the tyrosine-kinase family, and sis, belonging to the cellular-growth-factor family, were found to have higher signals (3.6-fold and 1.8-fold respectively) in MH-02 than in ML-01 cells. To explore the possibility that higher expression of these oncogenes is involved in enhanced metastasis of the MH-02 clone, ML-01 was transfected by a fyn vector and the metastatic potential of the transfectant was examined. Mice administered fyn-transfected ML-01 cells had significantly increased metastatic nodules in the lung, as compared with those whose ML-01 cells were transfected with control vector without the fyn gene. The result indicates that the fyn gene is one of the factors governing the metastatic potential of Meth A cells.
Takayama
T
T
Department of Internal Medicine (Section 4) Sapporo Medical College, Japan.
Mogi
Y
Y
Kogawa
K
K
Yoshizaki
N
N
Muramatsu
H
H
Koike
K
K
Semba
K
K
Yamamoto
T
T
Niitsu
Y
Y
D000818:Animals; D005354:Fibrosarcoma / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D009857:Oncogenes / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D051076:Proto-Oncogene Proteins c-fyn; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection; D014407:Tumor Cells, Cultured
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D008748:Methylcholanthrene; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn