The nifY product of Klebsiella pneumoniae is associated with apodinitrogenase and dissociates upon activation with the iron-molybdenum cofactor.
Apodinitrogenase, which lacks the iron-molybdenum cofactor at its active site, is an oligomer that contains an additional protein not found in the active dinitrogenase tetramer. This associated protein in Klebsiella pneumoniae is shown to be the product of the nifY gene. When apodinitrogenase is activated by the addition of the iron-molybdenum cofactor, NifY dissociates from the apodinitrogenase complex. The conditions for this dissociation are described. Finally, there are aspects of the dissociation and insertion process in K. pneumoniae that are different from that in Azotobacter vinelandii.
Homer
M J
MJ
Department of Bacteriology, University of Wisconsin, Madison 53706.
Paustian
T D
TD
Shah
V K
VK
Roberts
G P
GP
D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D003067:Coenzymes / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000737:chemistry* / Q000201:enzymology; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000378:metabolism; D009591:Nitrogenase / Q000032:analysis*
D001051:Apoenzymes; D001426:Bacterial Proteins; D003067:Coenzymes; C075094:NifY protein, Bacteria; D008982:Molybdenum; D007501:Iron; D009591:Nitrogenase