Gas chromatographic/chemiluminescence detection (thermal energy analyzer-nitrogen mode) method for the determination of dibutylamine in hams.
A gas chromatographic (GC) procedure is described for the determination of dibutylamine (DBA) in ham at less than 1 ppm. Sample is extracted with 0.5N HCl, centrifuged, made basic, and then extracted with isopropyl alcohol. Final determination is by GC/thermal energy analysis in the nitrogen mode. Six replicate analyses of ham fortified with 0.5 ppm DBA gave a recovery of 90.2 +/- 3.4%. Over a range of 0.5 to 3.0 ppm DBA, the repeatability was 0.07 ppm, and the coefficient of variation was 6.07%. Limit of detection was 0.1 ppm. Effectiveness of the procedure was demonstrated on selected ham samples.
Fiddler
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W
U.S. Department of Agriculture, Eastern Regional Research Center, Philadelphia, PA 19118.
Doerr
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RC
D000818:Animals; D002082:Butylamines / Q000032:analysis*; D002849:Chromatography, Gas; D004055:Differential Thermal Analysis; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008460:Meat / Q000032:analysis*; D013552:Swine
D002082:Butylamines; D007202:Indicators and Reagents; C045274:dibutylamine