A simple method of rapid freezing adequately preserves brain tissue for immunocytochemistry, light and electron microscopic examination.
A simple and reproducible method for cryopreservation of brain tissue from patients with Alzheimer's disease is described. Fresh brain slices (1 cm thick) obtained less than 6 h postmortem are placed in sealed plastic bags, sandwiched between 0.3-cm-thick aluminium sheets, and frozen by placing the entire "sandwich" between layers of dry ice pellets. The frozen brain slices are stored at -85 degrees C. Specific anatomic areas can be retrieved at any time for light and electron microscopic, immunocytochemical, autoradiographic and neurochemical studies.
Nochlin
D
D
Department of Pathology, University of Washington School of Medicine, Seattle 89195-0001.
Mackenzie
A P
AP
Bryant
E M
EM
Norwood
T H
TH
Sumi
S M
SM
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000032:analysis; D001921:Brain / Q000166:cytology* / Q000648:ultrastructure*; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D008875:Middle Aged; D016501:Neurites / Q000473:pathology / Q000648:ultrastructure; D013997:Time Factors; D016875:tau Proteins / Q000032:analysis
D016229:Amyloid beta-Peptides; D005904:Glial Fibrillary Acidic Protein; D016875:tau Proteins