Mapping genes according to their amplification status in tumor cells: contribution to the map of 11q13.
DNA amplification events that occur at 11q13 in human carcinomas are complex, and amplicons were shown to exhibit numerous discontinuities that could be used as tools for mapping this genomic area. A statistical nearest-neighbor analysis of the amplification patterns of several 11q13 loci in a panel of amplified DNAs was performed in the region of 11q13 centromeric from BCL1. It was strongly correlated with the physical linkages that we established between the same markers. Taken together, our results led to a map of 14 genes and four anonymous probes located in this area.
Szepetowski
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LGMCH, CNRS URA 1462, Nice, France.
Perucca-Lostanlen
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Gaudray
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P
D001943:Breast Neoplasms; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D005784:Gene Amplification; D008040:Genetic Linkage; D006801:Humans; D014407:Tumor Cells, Cultured
D015342:DNA Probes