Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in tissues by high performance liquid chromatography.
A procedure for the extraction and high performance liquid chromatographic (HPLC) determination of the photodynamic therapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin in human, rat and mouse tissues following intravenous administration of the drug is described. The tissue (tumour, skin, muscle and liver) was homogenized and extracted into a mixture of methanol:dimethyl sulphoxide:water (32:8:1 by vol.) containing, 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard. The precipitated proteins were removed by centrifugation and the supernatant was separated by reversed phase HPLC on a Hypersil-ODS column with 77% (v/v) acetonitrile in 0.1% trifluoroacetic acid as the mobile phase. The solute was detected with high sensitivity and specificity by a UV-VIS detector set at 423 nm.
Wang
Q
Q
MRC Toxicology Unit, Medical Research Council Laboratories, Carshalton, Surrey, UK.
Altermatt
H J
HJ
Ris
H B
HB
Reynolds
B E
BE
Stewart
J C
JC
Bonnett
R
R
Lim
C K
CK
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004121:Dimethyl Sulfoxide; D006801:Humans; D008099:Liver / Q000737:chemistry; D008652:Mesoporphyrins / Q000032:analysis*; D000432:Methanol; D051379:Mice; D009132:Muscles / Q000737:chemistry; D009369:Neoplasms / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000032:analysis*; D051381:Rats; D012867:Skin / Q000737:chemistry
D008652:Mesoporphyrins; D011838:Radiation-Sensitizing Agents; C072269:temoporfin; D000432:Methanol; D004121:Dimethyl Sulfoxide