title: Kin recognition between medial Golgi enzymes in HeLa cells.
abstract: The medial Golgi enzymes, N-acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, beta-1,4-galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack.
authors.affiliation: Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK.
authors.forename: T
authors.initials: T
authors.lastname: Nilsson
authors.forename: M H
authors.initials: MH
authors.lastname: Hoe
authors.forename: P
authors.initials: P
authors.lastname: Slusarewicz
authors.forename: C
authors.initials: C
authors.lastname: Rabouille
authors.forename: R
authors.initials: R
authors.lastname: Watson
authors.forename: F
authors.initials: F
authors.lastname: Hunte
authors.forename: G
authors.initials: G
authors.lastname: Watzele
authors.forename: E G
authors.initials: EG
authors.lastname: Berger
authors.forename: G
authors.initials: G
authors.lastname: Warren
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005700:Galactosyltransferases / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D008361:Mannosidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*
chemical_list: D004247:DNA; D005700:Galactosyltransferases; D017351:N-Acetylglucosaminyltransferases; C024850:alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase