title: The fate of cryopreserved nerve isografts and allografts in normal and immunosuppressed rats.
abstract: Donor Schwann cells, perineurial cells, and vasculature are known to survive in grafts of peripheral nerve. In the present study, we attempted to cryopreserve nerve to determine whether these cellular components of nerve would survive after transplantation and support host axonal regeneration through the graft. Four-centimeter lengths of peroneal nerves were removed from inbred adult American Cancer Institute (ACI) rats and placed into vials that contained a cryoprotective mixture of dimethyl sulfoxide and formamide (DF) at room temperature. Each vial with nerves in DF was cooled at a rate of 1-1.5 degrees C/minute down to -40 degrees C at which point the vials were plunged into liquid nitrogen at -196 degrees C. After 5 weeks of storage, the nerves were thawed and DF removed. Some of the cryopreserved-thawed ACI nerves were transplanted as isografts into the legs of ACI rats. Other ACI nerves were used as allografts and inserted into immunologically normal Fischer (FR) rats that were untreated or were immunosuppressed with the drug Cyclosporin A (Cy-A). At surgery, only one end of the nerve graft was joined to the cut proximal end of the peroneal nerve of the host. The cellular elements of ACI grafts were present at 5 weeks in grafts removed from ACI rats and FR rats treated with Cy-A. Non-immunosuppressed FR rats rejected ACI nerves as did FR rats in whom Cy-A was stopped after 5 weeks of treatment. All surviving ACI grafts underwent Wallerian degeneration and consisted of columns of Schwann cells, which in their proximal portion were associated with regenerating host axons. The donor perineurial sheath and vasculature were also present in surviving grafts. ACI isografts only were examined 20 weeks postoperatively. All normal tissue components survived in these older grafts and contained regenerated and myelinated host axons throughout their 4 cm lengths. These results demonstrated that the cellular elements of nerve can be cryopreserved, and after transplantation, survive and function. Because nerves survived after prolonged cryopreservation, it seems feasible to establish a nerve bank from which grafts can be withdrawn to repair gaps in injured nerves. However, cryopreserved nerves used as allografts remain immunogenic and require immunosuppression for their survival.
authors.affiliation: Laboratory of Neural Control, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: A A
authors.initials: AA
authors.lastname: Zalewski
authors.forename: G M
authors.initials: GM
authors.lastname: Fahy
authors.forename: N A
authors.initials: NA
authors.lastname: Azzam
authors.forename: R N
authors.initials: RN
authors.lastname: Azzam
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D015925:Cryopreservation; D016572:Cyclosporine / Q000494:pharmacology; D006084:Graft Rejection; D006085:Graft Survival / Q000502:physiology*; D007165:Immunosuppression Therapy; D008297:Male; D008854:Microscopy, Electron; D009416:Nerve Regeneration / Q000502:physiology; D009417:Nerve Tissue / Q000166:cytology / Q000637:transplantation*; D010543:Peroneal Nerve / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D051381:Rats; D012583:Schwann Cells / Q000502:physiology; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D014855:Wallerian Degeneration
chemical_list: D016572:Cyclosporine