title: Gas chromatography/mass spectrometric analysis of urinary nucleosides in cancer patients; potential of modified nucleosides as tumour markers.
abstract: Analysis of urine from cancer patients by capillary gas chromatography/mass spectrometry positively identified 14 urinary nucleosides including several modified nucleosides. Levels of the modified nucleosides 1-methyl-adenosine, 2-methylguanosine, N2,N2-dimethylguanosine and 1-methylinosine as well as the total nucleoside level were elevated in the urine when a malignant tumour was present; the levels of N2,N2-dimethylguanosine were found to correlate with the stage of the cancer.
authors.affiliation: Biochemistry Research Group (SoBS), University College of Swansea, UK.
authors.forename: J I
authors.initials: JI
authors.lastname: Langridge
authors.forename: T D
authors.initials: TD
authors.lastname: McClure
authors.forename: S
authors.initials: S
authors.lastname: el-Shakawi
authors.forename: A
authors.initials: A
authors.lastname: Fielding
authors.forename: K H
authors.initials: KH
authors.lastname: Schram
authors.forename: R P
authors.initials: RP
authors.lastname: Newton
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000652:urine; D014408:Biomarkers, Tumor; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006151:Guanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D007288:Inosine / Q000031:analogs & derivatives* / Q000652:urine; D008297:Male; D009369:Neoplasms / Q000652:urine*; D009705:Nucleosides / Q000652:urine*
chemical_list: D014408:Biomarkers, Tumor; D009705:Nucleosides; D006151:Guanosine; C002230:1-methyladenosine; C008940:N(2),N(2)-dimethylguanosine; C012433:1-methylinosine; D007288:Inosine; D000241:Adenosine