Treatment of gamma radiation-induced transplanted leukemia in ICRC mice by liposomally encapsulated 5-fluoro uracil.
The present investigation reports on the efficacy of 5-FU encapsulated in liposomes for the treatment of leukemia using a murine model of gamma radiation-induced transplantable leukemia in ICRC strain of mice. Multi-lamellar vesicles (MLVs) or large unilamellar vesicles (REVs) prepared using phosphatidyl choline and cholesterol in a molar ratio of 8:2 for neutral and 7:2:1 for charged vesicles were administered as a single i.p. dose in mice. 5-FU encapsulated in MLVs at a concentration of 0.6-2.5 mg/kg had no effect, whereas REVs at a single i.p. dose of 9 mg/kg increased survival of leukemic mice with T/C = 138%, decreased peripheral blood count and considerably reduced infiltration of leukemic cells in different tissues (supported by histopathology) as compared to 60 mg/kg of free drug (LD10 = 70 mg/kg).
Joshi
S V
SV
Chemotherapy Division, Cancer Research Institute, Tata Memorial Hospital, Tata Memorial Centre, Parel, Bombay, India.
Vaidya
S G
SG
Nerurkar
V R
VR
Soman
C
C
D000818:Animals; D002626:Chemistry, Pharmaceutical; D004195:Disease Models, Animal; D004337:Drug Carriers; D004355:Drug Stability; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage* / Q000633:toxicity; D005720:Gamma Rays; D007945:Leukemia, Lymphoid / Q000188:drug therapy* / Q000209:etiology; D007953:Leukemia, Radiation-Induced / Q000188:drug therapy* / Q000209:etiology; D008081:Liposomes; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009368:Neoplasm Transplantation
D004337:Drug Carriers; D008081:Liposomes; D005472:Fluorouracil