title: Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in tissues by high performance liquid chromatography.
abstract: A procedure for the extraction and high performance liquid chromatographic (HPLC) determination of the photodynamic therapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin in human, rat and mouse tissues following intravenous administration of the drug is described. The tissue (tumour, skin, muscle and liver) was homogenized and extracted into a mixture of methanol:dimethyl sulphoxide:water (32:8:1 by vol.) containing, 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard. The precipitated proteins were removed by centrifugation and the supernatant was separated by reversed phase HPLC on a Hypersil-ODS column with 77% (v/v) acetonitrile in 0.1% trifluoroacetic acid as the mobile phase. The solute was detected with high sensitivity and specificity by a UV-VIS detector set at 423 nm.
authors.affiliation: MRC Toxicology Unit, Medical Research Council Laboratories, Carshalton, Surrey, UK.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: H J
authors.initials: HJ
authors.lastname: Altermatt
authors.forename: H B
authors.initials: HB
authors.lastname: Ris
authors.forename: B E
authors.initials: BE
authors.lastname: Reynolds
authors.forename: J C
authors.initials: JC
authors.lastname: Stewart
authors.forename: R
authors.initials: R
authors.lastname: Bonnett
authors.forename: C K
authors.initials: CK
authors.lastname: Lim
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004121:Dimethyl Sulfoxide; D006801:Humans; D008099:Liver / Q000737:chemistry; D008652:Mesoporphyrins / Q000032:analysis*; D000432:Methanol; D051379:Mice; D009132:Muscles / Q000737:chemistry; D009369:Neoplasms / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000032:analysis*; D051381:Rats; D012867:Skin / Q000737:chemistry
chemical_list: D008652:Mesoporphyrins; D011838:Radiation-Sensitizing Agents; C072269:temoporfin; D000432:Methanol; D004121:Dimethyl Sulfoxide