title: The development of an enzyme-linked immunosorbent assay for the determination of cortisol binding globulin.
abstract: A simple, reliable and reproducible enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies for human cortisol binding globulin (CBG) has been developed. The sensitivity of the ELISA (1.20 fmol CBG/well) compared favourably with the sensitivity of other immunoassays. The excellent agreement (r = 0.98) seen between the present study and a binding assay indicates that the polyclonal antibodies used recognize only intact steroid-binding CBG. The intra- and inter-assay coefficient of variation (4.0% and 7.1% respectively) compared favourably with those reported by other authors.
authors.affiliation: Department of Human and Animal Physiology, University of Stellenbosch, South Africa.
authors.forename: A W
authors.initials: AW
authors.lastname: van Rijswijk
authors.forename: J C
authors.initials: JC
authors.lastname: Morgenthal
authors.forename: J J
authors.initials: JJ
authors.lastname: Taljaard
mesh_terms: D002352:Carrier Proteins / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C027091:cortisol binding globulin; D006854:Hydrocortisone