title: Determination of cisplatin and some possible metabolites by ion-pairing chromatography with inductively coupled plasma mass spectrometric detection.
abstract: A sensitive method is described for measuring cisplatin and some possible metabolites. The method combines reversed-phase ion-pairing liquid chromatography (LC) with inductively coupled plasma mass spectrometry (ICP-MS) for platinum-specific detection. Separation conditions for cisplatin hydrolysis products and the reaction products of cisplatin with methionine, cysteine, and glutathione have been investigated with sodium dodecylsulfate or heptanesulfonate as the ion-pairing agent. The detection limit for cisplatin was found to be 0.1 ng, corresponding to a concentration detection limit of 1 ng/ml when using an injection volume of 100 microliters. This study has demonstrated the usefulness of LC-ICP-MS for cisplatin metabolism studies.
authors.affiliation: Department of Chemistry, University of Cincinnati, OH 45221-0172.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.forename: K
authors.initials: K
authors.lastname: Tepperman
authors.forename: J G
authors.initials: JG
authors.lastname: Dorsey
authors.forename: R C
authors.initials: RC
authors.lastname: Elder
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000032:analysis*; D003545:Cysteine / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D006868:Hydrolysis; D013058:Mass Spectrometry; D008715:Methionine / Q000032:analysis; D009944:Organoplatinum Compounds / Q000032:analysis; D013438:Sulfhydryl Compounds / Q000032:analysis
chemical_list: D009944:Organoplatinum Compounds; D013438:Sulfhydryl Compounds; D008715:Methionine; D005978:Glutathione; D003545:Cysteine; D002945:Cisplatin