RNA editing of rapeseed mitochondrial atp9 transcripts: RNA editing changes four amino acids, but termination codon is already encoded by genomic sequence.
The gene encoding subunit 9 of Fo-ATPase of rapeseed mitochondria has been isolated. The complete genomic DNA sequence and cDNA sequence corresponding to the atp9 gene transcript have been determined by a method involving cDNA synthesis, using specific oligonucleotides as primers, followed by PCR amplification, cloning and sequencing of the amplification products. In comparison of cDNA sequences to genomic one, four modifications, C-to-U conversions, have been found. When compared with RNA editing patterns of atp9 transcripts among plant mitochondria, that of rapeseed atp9 transcript is more simple; there are only four editing sites on the coding region, and its termination codon is already encoded by genomic sequence.
Handa
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Department of Cell Biology, National Institute of Agrobiological Resources, Ibaraki, Japan.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D004272:DNA, Mitochondrial; D008928:Mitochondria / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011510:Proteolipids / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000235:genetics*; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D013728:Terminator Regions, Genetic
C063668:ATP9 protein, Arabidopsis; D000596:Amino Acids; D029681:Arabidopsis Proteins; D004272:DNA, Mitochondrial; D010940:Plant Proteins; D011510:Proteolipids; D012333:RNA, Messenger; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases