Mutation of the tumor suppressor gene TP53 is not detected in psoriatic skin.
We investigated the tumor-suppressor gene, TP53, in psoriatic skin at gene (GP53) and protein (p53) levels. No mutation was detected in the seven exons analyzed using a combination of polymerase chain reaction and single-strand conformation polymorphism techniques. In addition, by immunohistochemistry using three different anti-p53 antibodies, no positive staining, reported to be associated with mutations and/or abnormal expression of the TP53 gene, was observed in psoriatic skin biopsies, in contrast to recent reports. These results suggest that the proliferative status of psoriatic keratinocytes does not implicate the TP53 gene.
Molès
J P
JP
Laboratoire de Dermatologie Moléculaire, CNRS/CRBM, Montpellier, France.
Theillet
C
C
Basset-Sèguin
N
N
Guilhou
J J
JJ
D001483:Base Sequence; D004277:DNA, Single-Stranded; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D008968:Molecular Conformation; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011565:Psoriasis / Q000235:genetics*; D012867:Skin / Q000378:metabolism / Q000503:physiopathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D004277:DNA, Single-Stranded; D015335:Molecular Probes; D016159:Tumor Suppressor Protein p53