title: [Evaluation of DNA-HIV detection in various population groups].
abstract: BACKGROUND
  To evaluate the diagnostic use of Polymerase Chain Reaction (PCR) for the detection of HIV-DNA.
  
  
  METHODS
  We have investigated HIV-DNA in peripheral blood mononuclear cells of 150 individuals: 50 seronegative without recognized factors to acquire HIV infection, 50 seronegative that showed risk habits and 50 seropositive. For amplification we have used primers SK38/SK39 which amplify a conserved region of 114 bp from HIV's gag gen. The detection of the amplificate was performed using solution hybridization with 32P-labelled SK 19, 6% polyacrylamide gel electrophoresis and autoradiography.
  
  
  RESULTS
  We have detected HIV-DNA at every seropositive patient (100%), 6 samples from seronegative individuals that showed risk habits and 1 sample from non-risk seronegative individuals.
  
  
  CONCLUSIONS
  Polymerase chain reaction represents a diagnostic tool at HIV infection, specially for those patients where seroconversion can not be demonstrated by conventional methods.
authors.affiliation: Departamento de Microbiología (Facultad de Medicina), Universidad de Granada.
authors.forename: E
authors.initials: E
authors.lastname: Quirós
authors.forename: F
authors.initials: F
authors.lastname: García
authors.forename: T
authors.initials: T
authors.lastname: Cabezas
authors.forename: I
authors.initials: I
authors.lastname: González
authors.forename: M C
authors.initials: MC
authors.lastname: Bernal
authors.forename: M C
authors.initials: MC
authors.lastname: Maroto
authors.forename: G
authors.initials: G
authors.lastname: Piedrola
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: D004279:DNA, Viral
vernacular_title: Evaluación de la detección de ADN-VIH en diversos grupos de población.