title: The human C/EBP delta (CRP3/CELF) gene: structure and chromosomal localization.
abstract: In an attempt to identify C/EBP-like transcription factors expressed in the prostate, a cDNA homologous to the mouse C/EBP delta (CRP3) and the rat CELF gene was isolated. A genomic clone containing the entire C/EBP delta gene was isolated using a cDNA fragment as a probe. The gene was characterized by restriction mapping and sequence analysis. By fluorescence in situ hybridization, using the biotinylated genomic clone as a probe, the C/EBP delta gene was assigned to the pericentromeric region of human chromosome 8, most probably to 8q11. This chromosomal localization was confirmed by analysis of a panel of human x hamster somatic cell hybrid DNA samples with a C/EBP delta-specific STS. As a result, the C/EBP delta gene could be positioned between the PLAT and the MOS loci.
authors.affiliation: Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: C B
authors.initials: CB
authors.lastname: Cleutjens
authors.forename: C C
authors.initials: CC
authors.lastname: van Eekelen
authors.forename: H
authors.initials: H
authors.lastname: van Dekken
authors.forename: E M
authors.initials: EM
authors.lastname: Smit
authors.forename: A
authors.initials: A
authors.lastname: Hagemeijer
authors.forename: M J
authors.initials: MJ
authors.lastname: Wagner
authors.forename: D E
authors.initials: DE
authors.lastname: Wells
authors.forename: J
authors.initials: J
authors.lastname: Trapman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000235:genetics*; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D051381:Rats / Q000235:genetics; D015183:Restriction Mapping; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins