Cloning, sequencing and regulation of an mRNA encoding porcine interleukin-1 beta.
Interleukin 1 (IL-1) is a cytokine that mediates a variety of immunological responses. We report here the isolation of a porcine IL-1 beta cDNA from an alveolar macrophage library. The complete cDNA sequence is 1458 bp in length and has an open reading frame of 803 bp, encoding a 267-amino-acid (aa) protein with an approximate molecular size of 31 kDa. The porcine IL-1 beta cDNA sequence shows homologies of 82.0, 81.0, 74.0, and 74.0% with the bovine, sheep, human, and murine sequences in the protein coding regions, respectively. Northern blot analysis reveals a single 1.7-kb mRNA species that is differentially regulated by lipopolysaccharide, phorbol myristate acetate, and tumor necrosis factor but is not regulated by human recombinant IL-1. Porcine IL-1 beta is down-regulated by dexamethasone, polymyxin B, and IL-4 in a fashion kinetically similar to the other reported species.
Huether
M J
MJ
Department of Clinical and Population Sciences, University of Minnesota, St. Paul 55108.
Lin
G
G
Smith
D M
DM
Murtaugh
M P
MP
Molitor
T W
TW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007375:Interleukin-1 / Q000235:genetics*; D015847:Interleukin-4 / Q000494:pharmacology; D008264:Macrophages; D008969:Molecular Sequence Data; D011112:Polymyxin B / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D007166:Immunosuppressive Agents; D007375:Interleukin-1; D012333:RNA, Messenger; D015847:Interleukin-4; D003907:Dexamethasone; D011112:Polymyxin B