Autoradiographic study of [125I]epidermal growth factor-binding sites in the mesencephalon of control and parkinsonian brains post-mortem.
Epidermal growth factor (EGF) is assumed to act as a neurotrophic factor on dopaminergic nigrostriatal neurons in cell cultures and animal brain. This led us to consider its possible role in the pathophysiology of Parkinson's disease. An autoradiographic study of the distribution of EGF-binding sites was performed in the mesencephalon of controls and patients with Parkinson's disease, a neurodegenerative disease associated with dramatic damage to the mesostriatal dopaminergic neurons. Scatchard analysis revealed a single type of binding sites with a high affinity constant, in the various mesencephalic dopaminergic areas examined. The characteristics and density of [125I]EGF-binding sites were similar in controls and parkinsonian patients. This suggests that EGF receptors in the mesencephalon are unaffected in Parkinson's disease and may therefore contribute to the increased activity and survival of the remaining dopaminergic neurons.
Villares
J
J
Laboratoire de Médecine Expérimentale, INSERM U289, Hôpital de la Salpêtrière, Paris, France.
Faucheux
B
B
Strada
O
O
Hirsch
E C
EC
Agid
Y
Y
Javoy-Agid
F
F
D001345:Autoradiography; D066246:ErbB Receptors / Q000032:analysis*; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D008636:Mesencephalon / Q000737:chemistry*; D010300:Parkinson Disease / Q000378:metabolism*; D011180:Postmortem Changes; D011869:Radioligand Assay
D007457:Iodine Radioisotopes; D066246:ErbB Receptors