Cell cycle regulation of the c-Myc transcriptional activation domain.
The product of the c-myc gene (c-Myc) is a sequence-specific DNA-binding protein that has previously been demonstrated to be required for cell cycle progression. Here we report that the c-Myc DNA binding site confers cell cycle regulation to a reporter gene in Chinese hamster ovary cells. The observed transactivation was biphasic with a small increase in G1 and a marked increase during the S-to-G2/M transition of the cell cycle. This cell cycle regulation of transactivation potential is accounted for, in part, by regulatory phosphorylation of the c-Myc transactivation domain. Together, these data demonstrate that c-Myc may have an important role in the progression of cells through both the G1 and G2 phases of the cell cycle.
Seth
A
A
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01605.
Gupta
S
S
Davis
R J
RJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D016466:CHO Cells; D002453:Cell Cycle / Q000235:genetics*; D002460:Cell Line; D006224:Cricetinae; D004247:DNA; D005786:Gene Expression Regulation; D016259:Genes, myc; D007700:Kinetics; D008969:Molecular Sequence Data; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D012694:Serine; D004247:DNA