Nucleotide sequence of the rabbit gamma-preprotachykinin I cDNA.
We succeeded in amplifying tachykinin I specific cDNAs from cerebral tissue of guinea pig, rabbit, rat, hamster, trout and tupaia in the expected size range of 820-980 bp. The amplified 859 bp rabbit gamma-preprotachykinin I cDNA was sequenced and consisted of the whole 345 bp gamma-PPT I coding sequence including the substance P and neurokinin A coding regions and a 505 bp large 3'-nontranslated region. Both, molecular weight and sequence comparison emphasizes the very high phylogenetic age of the preprotachykinin I gene.
Mägert
H J
HJ
Lower Saxony Institute for Peptide-Research, Hannover, Germany.
Heitland
A
A
Rose
M
M
Forssmann
W G
WG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D011498:Protein Precursors / Q000235:genetics*; D011817:Rabbits / Q000235:genetics*; D015320:Tachykinins / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; D015320:Tachykinins; C044206:preprotachykinin; D004247:DNA