title: T-cell surface molecule expression and interferon-gamma production in human cord blood.
abstract: We investigated T-cell surface antigen expression and the production of interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) by human cord and adult blood cells. Cord blood lymphocytes stimulated with Staphylococcus enterotoxin B (SEB) released only minimal titers of IFN-gamma and IL-2 in comparison with adult blood lymphocytes. Systematic analyses of several cell-surface molecules on untreated cord blood and adult blood T cells demonstrated a significant reduction of CD8+ subset numbers in cord blood. Furthermore, cord blood lymphocytes did not express the memory T-cell marker CD45RO. No differences were found in gamma/delta T-cell receptor (TCR), CD5, and CD25 antigen expression between untreated cord blood and adult blood. However, cord blood mononuclear cells showed a decreased CD25 antigen expression in comparison to those of adults following activation with SEB. These observations suggest that the decrease in the proportion of some T-cell subsets and the absence of CD45RO memory T cells may be responsible for the diminished lymphokine production of cord blood lymphocytes.
authors.affiliation: Institute of Immunology and Transfusion Medicine, University of Lübeck Medical School, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Kruse
authors.forename: P
authors.initials: P
authors.lastname: Neustock
authors.forename: M
authors.initials: M
authors.lastname: Reuter
authors.forename: H
authors.initials: H
authors.lastname: Kirchner
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000096:biosynthesis*; D002478:Cells, Cultured; D004768:Enterotoxins / Q000494:pharmacology; D005312:Fetal Blood / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D007369:Interferon Inducers / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D013211:Staphylococcus aureus; D016176:T-Lymphocyte Subsets / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D004768:Enterotoxins; D007369:Interferon Inducers; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma