title: Rapid acceleration of neutrophil apoptosis by tumor necrosis factor-alpha.
abstract: We demonstrate here that human necrosis factor-alpha, a potent neutrophil activator, induces rapid (within 3 h) apoptosis of these cells, i.e. neutrophils treated with this cytokine exhibit (i) light and electron microscopic changes characteristic to apoptotic cells, (ii) reduced propidium iodide binding to DNA, and (iii) the ladder form of DNA, as shown by agarose gel electrophoresis. These results suggest that apoptosis acceleration may be involved in processes by which neutrophils are prevented from damaging tissues.
authors.affiliation: Department of Parasitology and Anatomy, Yamagata University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Yonehara
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: F
authors.initials: F
authors.lastname: Sendo
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D016207:Cytokines / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008854:Microscopy, Electron; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D011419:Propidium / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D011419:Propidium; D004247:DNA