Radioimmunoligand characterization and immunohistochemical localization of dopamine D2 receptors on rods in the rat retina.
The retinal neurotransmitter dopamine (DA), elaborated from intrinsic dopaminergic neurons as amacrine and interplexiform cells, is known to modulate several complex functions mediated by D1 and D2 receptors in the vertebrate retina. In this paper, we characterized and localized DA receptors of the D2 family on rod outer segments (ROS) of the rat retina by a radioimmunoligand binding assay and by immunohistochemistry. Anti-anti-DA conjugated antibodies (or anti-idiotypic antibodies Ab2) were used as ligand; BSA-glutaraldehyde-conjugated spiperone, eticlopride (D2 antagonists) and DA were used as displacers. The linear Scatchard transformation indicated that data were best fit to the one-site model. By using the peroxidase-antiperoxidase technique, an intense labeling was located on rods. These results supported the paracrine action of DA on the photoreceptor cell.
Vuvan
T
T
INSERM Unité de Physiopathologie de l'Oeil, Paris, France.
Geffard
M
M
Denis
P
P
Simon
A
A
Nguyen-Legros
J
J
D000818:Animals; D004298:Dopamine / Q000276:immunology / Q000502:physiology; D065127:Dopamine D2 Receptor Antagonists; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D010786:Photoreceptor Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000187:drug effects* / Q000276:immunology; D012457:Salicylamides / Q000493:pharmacokinetics; D013134:Spiperone / Q000493:pharmacokinetics
D065127:Dopamine D2 Receptor Antagonists; D007457:Iodine Radioisotopes; D017448:Receptors, Dopamine D2; D012457:Salicylamides; D013134:Spiperone; C045989:eticlopride; D004298:Dopamine