title: Alteration of DNA binding specificity by nickel (II) substitution in three zinc (II) fingers of transcription factor Sp1.
abstract: Transcription factor Sp1, which has a DNA binding domain composed of three zinc fingers, binds to GC box (consensus sequence, G/T-GGGCGG-G/A-G/A-C/T) and activates the transcription by RNA polymerase II. Metal substitution of nickel(II) for zinc(II) in Sp1 causes no differences in the mode of protein-DNA interaction. However, sequence preference of Ni(II)Sp1 changes from 5'-GGGGCGGGGC to 5'-GGGGCGTGGC, and is distinct from that of Zn(II)Sp1. The result indicates an important effect of metal-induced folding on sequence-specific recognition of DNA by zinc-finger proteins.
authors.affiliation: Institute for Chemical Research, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nagaoka
authors.forename: J
authors.initials: J
authors.lastname: Kuwahara
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiura
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009532:Nickel / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism*; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*; D016335:Zinc Fingers / Q000502:physiology*
chemical_list: D009841:Oligonucleotides; D016329:Sp1 Transcription Factor; D009532:Nickel; D015032:Zinc