title: Anterior chamber-associated immune deviation elicited via primate eyes.
abstract: OBJECTIVE
  To determine whether injection of a soluble antigen, ovalbumin (OVA), into the anterior chamber of cynomolgus monkey eyes would impair the ability of these animals to subsequently develop delayed hypersensitivity when confronted by this antigen in immunogenic form.
  
  
  METHODS
  OVA or phosphate-buffered saline was injected into the anterior chamber of adult cynomolgus monkeys that were subsequently immunized with OVA in adjuvant and then skin challenged for delayed hypersensitivity with OVA.
  
  
  RESULTS
  Recipients of intracameral OVA proved unable to acquire antigen-specific delayed hypersensitivity when they received an immunogenic regimen of OVA in adjuvant. Since the flow of aqueous humor through the uveoscleral pathway of primate eyes can be promoted by topical treatment with PGF2 alpha isopropylester, a preliminary experiment is described in which induction of anterior chamber-associated immune deviation by OVA was prevented when the antigen was first introduced into monkey eyes treated with PGF2 alpha isopropylester.
  
  
  CONCLUSIONS
  Monkeys resemble rodents in displaying anterior chamber associated immune deviation (impaired ability to acquire antigen-specific delayed hypersensitivity) when they first encounter an antigen via the anterior chamber. The findings suggest that the cellular and molecular mechanisms of immune privilege, recently described in rodents, may apply to immune responses to intraocular antigens and pathogens in primates, including humans. Primate eyes offer an opportunity to explore the mechanisms of anterior chamber-associated immune deviation using pharmacologic agents that modify the aqueous outflow tracts.
authors.affiliation: Anatomisches Institut II, Universitat Erlangen-Nurnberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Eichhorn
authors.forename: M
authors.initials: M
authors.lastname: Horneber
authors.forename: J W
authors.initials: JW
authors.lastname: Streilein
authors.forename: E
authors.initials: E
authors.lastname: Lutjen-Drecoll
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D015237:Dinoprost / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007114:Immunization; D007267:Injections; D008252:Macaca fascicularis; D010047:Ovalbumin / Q000037:antagonists & inhibitors / Q000276:immunology*; D012882:Skin Tests
chemical_list: D010047:Ovalbumin; D015237:Dinoprost