[Effects of protein kinase C activator and inhibitor on release of tumor necrosis factor from mouse peritoneal macrophages].
The effects of protein kinase C (PKC) activator 1-O-tetradecanoylphorbol-13-acetate (TPA) and inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) and quercetin were studied on release of tumor necrosis factor (TNF) from mouse peritoneal macrophages primed with Propionibacterium acnes (PA). The results showed that TPA (1-100 ng.ml-1) and lipopolysaccharides (LPS) (1-100 ng.ml-1) induced the release of TNF from PA-primed mouse peritoneal macrophages in dose- and time-dependent manners in vitro, and the effects of TPA and LPS were inhibited by H-7 (12.5-100 mumol.L-1) or quercetin (6.25-25 mumol.L-1) in a dose-dependent manner. After ip H-7 (50 mg.kg-1), LPS-induced release of TNF in vivo decreased significantly. These results suggest that PKC may play a critical role in release of TNF from PA-primed macrophages.
Hu
Z L
ZL
Research Laboratory of Natural and Synthetic Drugs, College of Pharmacy, Second Military Medical University, Shanghai, China.
Qian
D H
DH
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D005260:Female; D007546:Isoquinolines / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D010529:Peritoneal Cavity / Q000166:cytology; D010879:Piperazines / Q000494:pharmacology*; D011425:Propionibacterium acnes; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011794:Quercetin / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007546:Isoquinolines; D008070:Lipopolysaccharides; D010879:Piperazines; D014409:Tumor Necrosis Factor-alpha; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011794:Quercetin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate