Effects of various anti-T cell receptor antibodies on the development of type II collagen-induced arthritis in mice.
Recent genetic studies of David and coworkers suggest that subsets of T cells utilizing specific V beta TcR genes may play important roles in the susceptibility to collagen-induced arthritis (CIA). Hence, in vivo depletion of such T cell subsets may significantly affect the development of CIA. To address this possibility, we first examined the effects of in vivo treatments with various monoclonal antibodies (mAbs) that are specific for particular TcR V beta families on the induction of CIA. Results presented in this study demonstrated that treatments with either anti-V beta 6, anti-V beta 8 or anti-V beta 11 did not suppress the development of arthritis in collagen-immunized mice. While combined treatments with these V beta specific mAbs which resulted in the in vivo elimination of V beta 6+, V beta 8+ and V beta 11+ T cells were not very effective in preventing the onset of CIA, the severity of the arthritic disease was somewhat reduced in animals that had received the triad of anti-V beta mAbs. By contrast, depletion of T cells expressing the alpha beta receptors by in vivo treatments with a pan anti-alpha beta mAb significantly decreased the incidence of CIA. Therefore, although an effect on the development of CIA was achieved by in vivo treatments with a mAb that detects all alpha beta + T cells, the elimination of only a few subsets of T cells which included the V beta 6+, V beta 8+, and V beta 11+ cells did not profoundly alter the incidence of CIA.(ABSTRACT TRUNCATED AT 250 WORDS)
Hom
J T
JT
Lilly Research Laboratories, Indianapolis 46285.
Estridge
T
T
Cole
H
H
Gliszczynski
V
V
Bendele
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001168:Arthritis / Q000209:etiology / Q000276:immunology*; D001172:Arthritis, Rheumatoid; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology*; D003094:Collagen / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA / Q000276:immunology; D011247:Pregnancy; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D016037:Single-Blind Method; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D016693:Receptors, Antigen, T-Cell, alpha-beta; D003094:Collagen