Portable system for continuous ex vivo measurements of lactate.
The lactate profile in serum was monitored continuously in volunteers under physical exercise on a test bicycle. The assay was operated ex vivo by means of continuous sampling through a coaxial catheter. After dialysis online, lactate was converted by means of lactate oxidase immobilized to porous glass, and the depletion of oxygen was registered by means of a Clark electrode. Good agreement between the continuous monitoring and off-line analyses was found.
Håkanson
H
H
Department of Biotechnology, Chemical Centre, Lund, Sweden.
Kyröläinen
M
M
Mattiasson
B
B
D015374:Biosensing Techniques; D002374:Catalase / Q000378:metabolism; D015444:Exercise; D006801:Humans; D007773:Lactates / Q000097:blood*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D008991:Monitoring, Physiologic / Q000379:methods; D005082:Physical Exertion
D007773:Lactates; D006899:Mixed Function Oxygenases; D002374:Catalase; C021497:lactate 2-monooxygenase