Glucocorticoid modulation of neurotrophin expression in immortalized mouse hippocampal neurons.
To see whether glucocorticoid hormones can influence the regulation of neurotrophin expression in hippocampal neurons, we have used reverse-transcription followed by polymerase chain reaction to investigate changes in the mRNA levels of nerve growth factor (NGF) and neurotrophin-3 (NT-3) in immortalized hippocampal neurons after dexamethasone application. Our results show that NGF mRNA levels rise in both embryonic and postnatal neurons, but with different time courses, while NT-3 levels rise in the embryonic but not in the postnatally derived cell line. Modulation of NT expression by glucocorticoids may therefore be developmentally regulated.
Scully
J L
JL
Department of Physiology, University of Basel, Switzerland.
Otten
U
U
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001483:Base Sequence; D002461:Cell Line, Transformed; D003907:Dexamethasone / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D051379:Mice; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D020933:Neurotrophin 3; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
D015335:Molecular Probes; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D003907:Dexamethasone