title: Effect of vitamin A deficiency on the adhesion of rat corneal epithelium and the basement membrane complex.
abstract: OBJECTIVE
  To understand the underlying mechanisms responsible for the easy removal and sloughing of corneal epithelium in vitamin A deficiency.
  
  
  METHODS
  An animal model of vitamin A deficiency, the vitamin A-deficient rat (A-rat), transmission electron microscopy, computer-assisted morphometric analysis and indirect immunofluorescence were used to study the adhesion of rat corneal epithelium to its basement membrane with emphasis on structure and molecular composition of the anchoring structures such as the hemidesmosome and bullous pemphigoid antigen.
  
  
  RESULTS
  Transmission electron microscopy resolved numerous microseparations of the basal epithelial cell membrane from the basement membrane with intervening segmental basement membrane duplications and electron dense deposits. Morphometric analysis disclosed a statistically significant reduction in the frequency and size of hemidesmosomes. Four weeks after supplementing the diet with retinyl acetate (700 micrograms/week), significant reversal of these same structural abnormalities could be detected. Immunofluorescence staining for bullous pemphigoid antigen, a component of the adhesion complex, showed intense staining of the basal epithelial cytoplasm but weak and discontinuous staining of the basement membrane. Weak staining for laminin was also evident in A- corneas. In contrast, normal corneas displayed no cytoplasmic staining for bullous pemphigoid antigen and intense staining of the basement membrane for bullous pemphigoid antigen and laminin.
  
  
  CONCLUSIONS
  The authors propose that structural abnormalities of the epithelial basement membrane complex are responsible for the observed loose epithelial adhesion and sloughing, as well as other known abnormalities of healing in the vitamin A-deficient rat cornea.
authors.affiliation: Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114.
authors.forename: N B
authors.initials: NB
authors.lastname: Shams
authors.forename: L A
authors.initials: LA
authors.lastname: Hanninen
authors.forename: H V
authors.initials: HV
authors.lastname: Chaves
authors.forename: G
authors.initials: G
authors.lastname: Frangieh
authors.forename: C V
authors.initials: CV
authors.lastname: Reddy
authors.forename: D T
authors.initials: DT
authors.lastname: Azar
authors.forename: K R
authors.initials: KR
authors.lastname: Kenyon
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure*; D002352:Carrier Proteins; D002448:Cell Adhesion; D003094:Collagen; D003315:Cornea / Q000378:metabolism / Q000648:ultrastructure*; D003598:Cytoskeletal Proteins; D004032:Diet; D004195:Disease Models, Animal; D000071821:Dystonin; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007797:Laminin / Q000378:metabolism; D008297:Male; D008858:Microscopy, Phase-Contrast; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D051381:Rats; D017207:Rats, Sprague-Dawley; D014802:Vitamin A Deficiency / Q000378:metabolism / Q000473:pathology*; D000097805:Collagen Type XVII
chemical_list: D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D007797:Laminin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C095667:Dst protein, mouse