The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region.
A chimeric cDNA was constructed, coding for the N-terminal region of BS-RNase (residues 1-49) and the C-terminal region of RNase A (residues 50-124). The resulting chimeric DNA was expressed in Escherichia coli and found to code for RNase chains that spontaneously assembled into a covalently dimeric ribonuclease.
Di Donato
A
A
Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Italy.
Cafaro
V
V
de Nigris
M
M
Rizzo
M
M
D'Alessio
G
G
D000818:Animals; D002417:Cattle; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000235:genetics; D012260:Ribonucleases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D012661:Semen / Q000201:enzymology*
D011993:Recombinant Fusion Proteins; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic