The 73-kb pIAA plasmid increases competitive fitness of Pseudomonas syringae subspecies savastanoi in oleander.
Pseudomonas syringae subsp. savastanoi causes tumors on olive and oleander by producing the plant growth regulators indoleacetic acid (IAA) and cytokinins following infection of the plant. The contribution of IAA production to the ability of P. syringae subsp. savastanoi to grow and survive in oleander leaf tissue was studied. Bacterial strains differing only with respect to IAA production were characterized. Growth and survival of wild-type and two mutant strains of P. syringae subsp. savastanoi in oleander leaf tissue were monitored by weekly colony counts and IAA plate assays. Growth rate of the three strains in culture and in planta did not differ significantly. However, the wild-type strain reached a higher population density and maintained its maximum density at least 9 weeks longer than either mutant population. An insertion mutant containing the IAA plasmid (pIAA), but incapable of IAA production, did not maintain a higher population density than a strain cured of the IAA plasmid. The pIAA-cured strain maintained a higher population density when coinoculated with an IAA-producing strain than when inoculated alone. These results suggest that IAA production may contribute to the fitness of P. syringae subsp. savastanoi in oleander tissue and that the iaa operon alone may be responsible for the competitive advantage of cells harboring pIAA.
Silverstone
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SE
Department of Biology, California State University, Bakersfield 93311.
Gilchrist
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DG
Bostock
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RM
Kosuge
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T
D005075:Biological Evolution; D004352:Drug Resistance, Microbial; D007210:Indoleacetic Acids / Q000378:metabolism*; D010944:Plants / Q000382:microbiology*; D010957:Plasmids; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015183:Restriction Mapping; D014364:Tryptophan / Q000031:analogs & derivatives / Q000494:pharmacology
D007210:Indoleacetic Acids; C020774:alpha-methyltryptophan; C030737:indoleacetic acid; D014364:Tryptophan