Algal phospholipids by 31P NMR: comparing isopropanol pretreatment with simple chloroform/methanol extraction.
1. A modified Folch procedure [potassium (ethylenedinitrilo)-tetraacetic acid at pH 6 substituted for KCl] is suitable for the extraction of marine algae. 2. The quantitative 31P nuclear magnetic resonance phospholipid profiles of four marine algae, Gracilaria verrucosa, Bryothamnion triquetrum, Padina gymnospora, and Caulerpa sertularioides, were obtained from Folch and Nichols extractions of both fresh and dried algae, and essentially identical results were obtained using either extraction procedure. 3. Extracts of air-dried algae are statistically different when compared to extracts of living algae, suggesting that tissue handling is a critical factor in phospholipid extractions.
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MR Laboratory, Chicago College of Osteopathic Medicine, IL 60615.
Navarro
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Glonek
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D000433:1-Propanol / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry; D056890:Eukaryota / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D000459:Phaeophyceae / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification*; D000461:Rhodophyta / Q000737:chemistry
D010743:Phospholipids; D002725:Chloroform; D000433:1-Propanol; D000432:Methanol