title: Phospholipid asymmetry of the outer membrane of rat liver mitochondria. Evidence for the presence of cardiolipin on the outside of the outer membrane.
abstract: The phospholipid topology of the outer membrane of intact rat liver mitochondria and derived outer membrane vesicles was investigated by determining the accessible pool of the various phospholipid classes towards phospholipase A2, a phosphatidylcholine-specific transfer protein and by chemical labeling using trinitrobenzene sulfonic acid. The outer membrane vesicles are sealed and have a right-side-out topology with a proposed localization of 55%, 77%, 100%, and at least 30% of the phosphatidylcholine, phosphatidylethanolamine, cardiolipin, and phosphatidylinositol plus phosphatidylserine in the outer leaflet, respectively. The outer membrane in intact mitochondria appears to have a similar phospholipid distribution.
authors.affiliation: Institute of Molecular Biology and Medical Biotechnology, Utrecht University, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Hovius
authors.forename: J
authors.initials: J
authors.lastname: Thijssen
authors.forename: P
authors.initials: P
authors.lastname: van der Linden
authors.forename: K
authors.initials: K
authors.lastname: Nicolay
authors.forename: B
authors.initials: B
authors.lastname: de Kruijff
mesh_terms: D000818:Animals; D002308:Cardiolipins / Q000032:analysis*; D007425:Intracellular Membranes / Q000737:chemistry; D008297:Male; D008930:Mitochondria, Liver / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002308:Cardiolipins; D010743:Phospholipids; D010741:Phospholipases A; D054467:Phospholipases A2