Simultaneous determination of a new anticancer drug galocitabine and its metabolites in blood by high-performance liquid chromatography.
A relatively simple and sensitive high-performance liquid chromatographic (HPLC) method is described for measuring galocitabine (Ro 09-1390) and its meatbolites, i.e. 5'-deoxy-fluorocytidine (5'-DFCR), 5'-deoxy-fluorouridine (5'-DFUR) and 5-fluorouracil (5-FU), in blood for the purpose of studying pharmacokinetics and toxicokinetics in small animals. The procedure for blood includes deproteinization with acetonitrile. Blood components were separated on a reversed-phase C18 column with a linear gradient of acetonitrile and water and detected at a wavelength of 270 nm. The between-day relative standard deviation (RSD) was less than 10% for all compounds at concentrations of 10-100 micrograms ml-1. The calibration curves obtained from the analysis of blood samples were linear and the correlation coefficients ranged from 0.997 to 0.999. The calculated determination limits were 6.9 micrograms ml-1 for galocitabine, 3.0 micrograms ml-1 for 5'-DFCR, 4.0 micrograms ml-1 for 5'-DFUR and 3.7 micrograms ml-1 for 5-FU.
Funaki
T
T
Nippon Roche Research Center, Kanagawa, Japan.
Onodera
H
H
Ogawa
K
K
Ichihara
S
S
Fukazawa
H
H
Kuruma
I
I
D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity; D005467:Floxuridine / Q000097:blood; D005472:Fluorouracil / Q000097:blood; D007202:Indicators and Reagents; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D005467:Floxuridine; D003841:Deoxycytidine; C063993:Ro 09-1390; D005472:Fluorouracil; C025034:doxifluridine