title: Multipin peptide synthesis at the micromole scale using 2-hydroxyethyl methacrylate grafted polyethylene supports.
abstract: The multipin peptide synthesis procedure has been adapted to allow the synthesis of peptides at micromole loadings. The original solid pin support was replaced with a detachable crown-shaped polyethylene support with an increased surface area. In addition, the polyethylene crowns were radiation-grafted with 2-hydroxyethyl methacrylate monomer instead of acrylic acid to yield hydroxy functionalized supports with a larger concentration of polymer and hence a larger peptide capacity. Fmoc-beta-Alanine was directly esterified to the HEMA hydroxy groups with subsequent addition of a diketopiperazine-forming handle for peptide attachment. Peptides varying in length from 10 to 25 residues were assembled at a number of loadings from 1.0 to 2.2 mumol. Purity of peptides at all loadings was equal to, and in some instances superior to, that achieved on conventional solid-phase supports.
authors.affiliation: Chiron Mimotopes Pty Ltd., Clayton, Victoria, Australia.
authors.forename: R M
authors.initials: RM
authors.lastname: Valerio
authors.forename: A M
authors.initials: AM
authors.lastname: Bray
authors.forename: R A
authors.initials: RA
authors.lastname: Campbell
authors.forename: A
authors.initials: A
authors.lastname: Dipasquale
authors.forename: C
authors.initials: C
authors.lastname: Margellis
authors.forename: S J
authors.initials: SJ
authors.lastname: Rodda
authors.forename: H M
authors.initials: HM
authors.lastname: Geysen
authors.forename: N J
authors.initials: NJ
authors.lastname: Maeji
mesh_terms: D000595:Amino Acid Sequence; D005069:Evaluation Studies as Topic; D008689:Methacrylates; D008722:Methods; D008832:Microchemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011095:Polyethylenes
chemical_list: D008689:Methacrylates; D009842:Oligopeptides; D010455:Peptides; D011095:Polyethylenes; C005044:hydroxyethyl methacrylate