[Amplification of the integrated bovine growth hormone transgene using the polymerase chain reaction (PCR)].
Conditions of the PCR amplification of unique fragments of bovine growth hormone (bGH) were found that allow reliable identification of transgenic rabbits for this gene. An integrated construction containing a part of MT promoter of metallothionein gene and chromosomal bGH gene was amplified. Two of 46 newborn lambs were found to be transgenic for the bGH gene by using primers BG3 and BG4 for amplification of the gene bGH fragment and its following restriction with pVUII. We managed to whow the possibility of successful amplification of growth hormones genes of pig and sheep using primers BG3 and BG8, corresponding respectively to the beginning and the end of the bGH gene.
Zhadanov
A B
AB
Dvorianchikov
G A
GA
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002417:Cattle; D004277:DNA, Single-Stranded; D013006:Growth Hormone / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011817:Rabbits; D015183:Restriction Mapping; D012756:Sheep
D004277:DNA, Single-Stranded; D013006:Growth Hormone; D008668:Metallothionein
Amplifikatsiia integrirovannogo transgena gena gormona rosta byka metodom polimeraznoĭ tsepnoĭ reaktsii (PCR).