A standard protocol for phototoxicity testing. Results from an interlaboratory study.
Based on experience accumulated in a number of laboratories, a standardized protocol for phototoxicity testing in experimental animals by the dermal route is presented. By conducting a limited interlaboratory study, demonstrating a high degree of consistency using 8-methoxypsoralen (8-MOP) and acridine, the sensitivity and reproducibility of the proposed methodology is demonstrated. Important aspects of performing phototoxicity testing are discussed.
Nilsson
R
R
Department of Genetic and Cellular Toxicology, Wallenberg Laboratory, University of Stockholm.
Maurer
T
T
Redmond
N
N
D000166:Acridines / Q000008:administration & dosage / Q000633:toxicity; D000279:Administration, Cutaneous; D000818:Animals; D002985:Clinical Protocols; D017484:Dermatitis, Phototoxic / Q000175:diagnosis* / Q000209:etiology; D006168:Guinea Pigs; D008297:Male; D008730:Methoxsalen / Q000008:administration & dosage / Q000633:toxicity; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000175:diagnosis / Q000209:etiology; D012882:Skin Tests / Q000379:methods / Q000592:standards*
D000166:Acridines; D008730:Methoxsalen