Nucleotide sequence and characterization of the traABCD region of IncI1 plasmid R64.
A 3.6-kb BglII-SmaI segment of the transfer region of IncI1 plasmid R64drd-11 was sequenced and characterized. Analysis of the DNA sequence indicated the presence of four genes, traA, traB, traC, and traD, in this region. The expression of the traB, traC, and traD genes was examined by maxicell experiments and that of the traA gene was examined by constructing the traA-lacZ fusion gene. The introduction of frameshift mutations into the four genes indicated that the traB and traC genes are essential for conjugal transfer in liquid medium and on a solid surface. Both were also required for the formation of the thin pilus, which is the receptor for phages I alpha and PR64FS. Upstream of the traA gene, a promoter sequence for sigma 70 of E. coli RNA polymerase was identified by S1 nuclease mapping and primer extension experiments.
Kim
S R
SR
Department of Biology, Tokyo Metropolitan University, Japan.
Funayama
N
N
Komano
T
T
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000235:genetics; D001426:Bacterial Proteins; D001483:Base Sequence; D003227:Conjugation, Genetic / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D016368:Frameshift Mutation; D005798:Genes, Bacterial / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011815:R Factors / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C444040:F pilin, E coli; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C082576:traB protein, Bacteria; C074790:traC protein, Plasmid F; C064957:traD protein, E coli; D036781:Fimbriae Proteins