title: Improved chromatographic purification of pea seedlings diamine oxidase.
abstract: An improved and simplified purification procedure has been developed for the isolation of diamine oxidase from pea seedlings (DAO EC 1.4.3.6). It involves ammonium sulphate precipitation, hydrophobic interaction chromatography, ion-exchange chromatography and size-exclusion chromatography. The homogeneity of the final enzyme preparations and molecular weight were determined by size-exclusion chromatography and by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS PAGE). The isoelectric point of 7.35 +/- 0.05 was determined by chromatofocusing and by polyacrylamide gel isoelectric focusing.
authors.affiliation: Department of Biochemistry, Masaryk University, Brno, Czechoslovakia.
authors.forename: M
authors.initials: M
authors.lastname: Wimmerová
authors.forename: Z
authors.initials: Z
authors.lastname: Glatz
authors.forename: O
authors.initials: O
authors.lastname: Janiczek
authors.forename: L
authors.initials: L
authors.lastname: Macholán
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000201:enzymology*; D007526:Isoelectric Point; D008970:Molecular Weight; D010946:Plants, Medicinal
chemical_list: D006631:Amine Oxidase (Copper-Containing)