title: Antibody responses of BALB/c mice to salivary antigens of hematophagous black flies (Diptera: Simuliidae).
abstract: The humoral antibody responses to salivary antigens of Simulium vittatum Zetterstedt were investigated in a BALB/c mouse laboratory model. Production of antisera was stimulated by intraperitoneal immunization with salivary gland extract or by feeding flies directly on depilated mice. Antibody responses in these two groups of mice were compared by western blotting, thus characterizing "true" salivary immunogens present in salivary gland extract. Immunized mice developed IgG, IgM, and IgE antibodies which recognized several salivary gland components, ranging in molecular weight between 26 and 67 kDa. Sera from bitten mice recognized fewer antigens, indicating that some components of the salivary gland extract were poorly immunogenic or absent from the saliva secreted during blood feeding. Antisera raised against S. vittatum also were used to identify cross-reactive immunogens and allergens in salivary gland extracts from other New World simuliids (Simulium argus Williston, S. metallicum Bellardi, and S. ochraceum Walker). SDS-PAGE protein profiles indicated a high degree of similarity between salivary gland extract of S. vittatum and S. argus, and several cross-reacting antigens were identified by western blotting. In contrast, protein profiles of S. ochraceum and S. metallicum differed from the former species, both qualitatively and quantitatively. Antisera demonstrated a low degree of cross-reactivity against salivary gland extract of S. ochraceum, whereas no cross-reactivity was detected against S. metallicum. These observations were confirmed using a monoclonal antibody raised against S. vittatum salivary gland extract (designated SVSG.1.F10), which showed cross-reactivity against S. argus but failed to recognize salivary gland components of either S. ochraceum or S. metallicum.
authors.affiliation: Department of Veterinary Sciences, University of Arizona, Tucson 85721.
authors.forename: M L
authors.initials: ML
authors.lastname: Cross
authors.forename: M S
authors.initials: MS
authors.lastname: Cupp
authors.forename: E W
authors.initials: EW
authors.lastname: Cupp
authors.forename: F B
authors.initials: FB
authors.lastname: Ramberg
authors.forename: F J
authors.initials: FJ
authors.lastname: Enriquez
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D005247:Feeding Behavior; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D012463:Saliva / Q000276:immunology*; D012469:Salivary Glands / Q000276:immunology*; D012843:Simuliidae / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal