Inhibition of rat liver hydroxysteroid sulfotransferase activity by alkylamines.
Triethylamine, which was used as an elution solvent for column chromatography to purify chemically synthesized 3'-phosphoadenosine 5'-phosphosulfate (PAPS), was a potent inhibitor of rat liver sulfotransferase (ST) activities toward androsterone and dehydroepiandrosterone, but not ST activities toward cortisol and 2-naphthol. Examination of fourteen primary, secondary and tertiary amines revealed that a secondary amine, di-n-butylamine, and three tertiary amines, triethylamine, tri-n-propylamine and tri-n-butylamine, specifically inhibited ST activities toward androgen.
Matsui
M
M
Kyoritsu College of Pharmacy, Tokyo, Japan.
Takahashi
M
M
Homma
H
H
D000588:Amines / Q000494:pharmacology*; D000738:Androsterone / Q000378:metabolism; D000818:Animals; D002082:Butylamines / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D003687:Dehydroepiandrosterone / Q000378:metabolism; D005021:Ethylamines / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D010724:Phosphoadenosine Phosphosulfate / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000037:antagonists & inhibitors*
D000588:Amines; D002082:Butylamines; D005021:Ethylamines; C045274:dibutylamine; D003687:Dehydroepiandrosterone; D010724:Phosphoadenosine Phosphosulfate; D000738:Androsterone; C036355:tributylamine; D013466:Sulfurtransferases; D015238:Sulfotransferases; C028152:alcohol sulfotransferase; C016162:triethylamine