Luminescence studies with trp repressor and its single-tryptophan mutants.
Time-resolved and steady-state fluorescence, low-temperature phosphorescence, and optically detected magnetic resonance (ODMR) measurements have been made to resolve the luminescence contributions of the two intrinsic tryptophan residues in the subunits of trp aporepressor from Escherichia coli. Assignments of spectral information have been confirmed by use of the single-tryptophan mutants W19F and W99F. Solute fluorescence quenching studies show that both Trp19 and Trp99 are exposed to acrylamide and iodide, with Trp99 being the more exposed. Time-resolved and steady-state fluorescence measurements show Trp19 to have a bluer emission, a longer mean fluorescence decay time, a higher quantum yield, and essentially no independent rotational motion with respect to the protein. Trp99 is found to have a redder emission, a shorter mean fluorescence decay time, a lower quantum yield, and a significant degree of rotational freedom. Phosphorescence studies show a clear resolution of 0-0 vibronic transitions for each type of residue, with maxima at 407 and 415 nm that are assigned to Trp19 and Trp99, respectively. ODMR measurements show the zero-field splitting parameters to be quite characteristically different for each tryptophan residue. The existence of resonance energy transfer from Trp19 to Trp99, in the wild-type protein, is indicated by three types of data: comparison of the long-lived decay time (attributed to Trp19) in the absence (W99F) and presence (wild type) of the acceptor Trp99, comparison of the fluorescence quantum yield of the wild-type and mutant proteins, and deviations from the expected phosphorescence intensities for Trp19 and Trp99 in the absence of energy transfer.
Eftink
M R
MR
Department of Chemistry, University of Mississippi, University 38677.
Ramsay
G D
GD
Burns
L
L
Maki
A H
AH
Mann
C J
CJ
Matthews
C R
CR
Ghiron
C A
CA
D001059:Apoproteins / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D001426:Bacterial Proteins; D003080:Cold Temperature; D004735:Energy Transfer; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D029968:Escherichia coli Proteins; D005453:Fluorescence; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009154:Mutation; D011193:Potassium Iodide / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry; D012097:Repressor Proteins / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D013050:Spectrometry, Fluorescence / Q000379:methods; D013329:Structure-Activity Relationship; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry* / Q000235:genetics
D001059:Apoproteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; C027190:TRPR protein, E coli; D011193:Potassium Iodide; D014364:Tryptophan