title: Is an activator protein-2-like transcription factor involved in regulating gene expression during nitrogen limitation in fungi?
abstract: The upstream sequences of all published lignin peroxidase and manganese peroxidase genomic clones from Phanerochaete chrysosporium were analyzed. This analysis revealed the presence of putative activator protein-2 (AP-2) recognition sequences in 11 of 15 lignin peroxidase genes. The lignin peroxidase clone GLG6 and the manganese peroxidase gene (mnp-1) have two copies of putative AP-2 sequence in the upstream region. Interestingly, the lignin peroxidase gene VLG4 of another white rot fungus, Trametes versicolor, and the nit-2 gene of Neurospora crassa also contain putative AP-2-binding sequences. Since all of these genes are regulated by nutrient nitrogen, I hypothesize that an AP-2-like transcription factor may be involved in inducing gene expression during nitrogen limitation in fungi.
authors.affiliation: Indiana University Purdue University, Fort Wayne, Indiana 46805.
authors.forename: S S
authors.initials: SS
authors.lastname: Dhawale
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000172:deficiency; D010544:Peroxidases / Q000235:genetics*; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D009584:Nitrogen