title: Electrophoretic techniques for the analysis of synthetic amyloid beta-A4-related peptides.
abstract: Two electrophoretic techniques have been developed for the analysis of synthetic beta-A4 related peptides. The first is an acetic acid--urea--polyacrylamide gel electrophoresis denaturing system while the second employs capillary zone electrophoresis at pH 7. Both methods were calibrated to identify the state (monomeric or aggregate) of the longer amyloid fragments beta-A4(1-40) and beta-A4(1-43) during analysis.
authors.affiliation: SmithKline Beecham Pharmaceuticals, Welwyn, Herts, United Kingdom.
authors.forename: P J
authors.initials: PJ
authors.lastname: Sweeney
authors.forename: J G
authors.initials: JG
authors.lastname: Darker
authors.forename: W A
authors.initials: WA
authors.lastname: Neville
authors.forename: J
authors.initials: J
authors.lastname: Humphries
authors.forename: P
authors.initials: P
authors.lastname: Camilleri
mesh_terms: D000085:Acetates; D019342:Acetic Acid; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry; D011487:Protein Conformation; D011489:Protein Denaturation; D014508:Urea
chemical_list: D000085:Acetates; D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D014508:Urea; D019342:Acetic Acid