[Transforming growth factor-beta (TGF-beta) inhibits epithelial wound healing of organ-cultured rabbit cornea].
Transforming growth factor-beta (TGF-beta) is a unique polypeptide growth factor which has been found to stimulate mesenchymal cell proliferation and inhibit epithelial cell growth. Whether or not TGF-beta inhibits corneal epithelial wound closure remains controversial. This study evaluated the effects of TGF-beta on corneal epithelial wound healing in vitro. Rabbit corneas with an artificial 8 mm epithelial defect were organ-cultured for 48 hr in TC-199 supplemented with TGF-beta (100 pg/ml, 1 ng/ml, or 10 ng/ml). The contralateral eye served as control. TGF-beta at 1 ng/ml and 10 ng/ml concentrations showed significant decrease in wound-healing rate compared with the control. 5-bromo-2'-deoxyuridine-immunohistochemistry showed a significant decrease in S-phase cell count in the 1 ng/ml group compared with the control. These results strongly suggest that TGF-beta inhibits corneal epithelial wound healing.
Nishida
K
K
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Ohashi
Y
Y
Nezu
E
E
Yamamoto
S
S
Kinoshita
S
S
Fujita
K
K
D000818:Animals; D003315:Cornea / Q000187:drug effects* / Q000503:physiopathology; D004848:Epithelium / Q000187:drug effects / Q000503:physiopathology; D009924:Organ Culture Techniques; D011817:Rabbits; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D016212:Transforming Growth Factor beta