[Purification and properties of lectins of Polygonatum multiflorum [L.] All. and Polygonatum verticillatum [L.] All].
The lectins from rootstocks of Polygonatum multiflorum [L.]. All. and P. verticillatum [L.] All. were purified by affinity chromatography on thyroglobulin-sepharose. Purified lectins were obtained from fresh raw material of P. multiflorum and P. verticillatum, 340 mg/kg and 120 mg/kg, respectively. The both lectins do not contain carbohydrates. Amino acid analysis has shown that the both lectins had high content of asparaginic acid/the lectin from P. verticillatum almost 28%. Ion-exchange chromatography permitted separating the lectin from P. multiflorum into 8 isoforms, that did not differ in molecular weight. Purified lectins interacted with sialic acid and did not interact with other monosaccharides. Purified lectins agglutinated rabbit and rat erythrocytes best of all, they less agglutinated mice and horse erythrocytes, but did not agglutinate human, cow, sheep and frog erythrocytes at all. Lyophylized lectins are amorphous white powders, that are well soluble in distilled water. Scientific-production co-operative "Lectinotest" began serial production of lectin from Polygonatum multiflorum from 1992 year.
Antoniuk
V O
VO
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification*; D009005:Monosaccharides / Q000737:chemistry; D037121:Plant Lectins; D010946:Plants, Medicinal / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry; D012995:Solubility; D013045:Species Specificity
D000596:Amino Acids; D037102:Lectins; D009005:Monosaccharides; D037121:Plant Lectins; D012794:Sialic Acids
Ochyshchennia ta vlastyvosti lektyniv kupyny bahatokvitkovoï (Polygonatum multiflorum [L.] All.) ta kupyny kil'chastoï (Polygonatum verticillatum [L.] All.).