title: [A method for the quantification of interleukin-4 in serum (sandwich ELISA) and IL-4 levels in patients with atopic dermatitis].
abstract: We have developed a sandwich enzyme-linked immunosorbent assay (ELISA) using chemiluminescent enzyme substrate AMPPD for the quantification of interleukin 4 (IL-4) in serum. IL-4 in serum could be measured in concentrations from 0.17 pg/ml. The IL-4 levels in patients with atopic dermatitis were significantly higher than those in normal controls.
authors.affiliation: Department of Dermatology, Nagoya City University Medical School.
authors.forename: M
authors.initials: M
authors.lastname: Kasamatsu
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Miura
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003876:Dermatitis, Atopic / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000097:blood*; D008297:Male; D008875:Middle Aged
chemical_list: D015847:Interleukin-4