title: Recombinant human brain-derived neurotrophic factor (rHuBDNF). Disulfide structure and characterization of BDNF expressed in CHO cells.
abstract: Three disulfide linkages of recombinant human brain-derived neurotrophic factor (BDNF) were determined by peptide sequence analysis and characterized by mass spectrometry. The three disulfide bonds for BDNF expressed in Chinese hamster ovary cells include Cys-13-Cys-80, Cys-58-Cys-109 and Cys-68-Cys-111, and the disulfide structure was homologous to that of nerve growth factor.
authors.affiliation: Department of Protein Structure, Amgen Inc., Thousand Oaks, California.
authors.forename: C
authors.initials: C
authors.lastname: Acklin
authors.forename: K
authors.initials: K
authors.lastname: Stoney
authors.forename: R A
authors.initials: RA
authors.lastname: Rosenfeld
authors.forename: J A
authors.initials: JA
authors.lastname: Miller
authors.forename: M F
authors.initials: MF
authors.lastname: Rohde
authors.forename: M
authors.initials: M
authors.lastname: Haniu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D016466:CHO Cells; D006224:Cricetinae; D003553:Cystine / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000737:chemistry* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D017421:Sequence Analysis; D013820:Thermolysin / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D003553:Cystine; D013820:Thermolysin