[A method for the quantification of interleukin-4 in serum (sandwich ELISA) and IL-4 levels in patients with atopic dermatitis].
We have developed a sandwich enzyme-linked immunosorbent assay (ELISA) using chemiluminescent enzyme substrate AMPPD for the quantification of interleukin 4 (IL-4) in serum. IL-4 in serum could be measured in concentrations from 0.17 pg/ml. The IL-4 levels in patients with atopic dermatitis were significantly higher than those in normal controls.
Kasamatsu
M
M
Department of Dermatology, Nagoya City University Medical School.
Tsuji
T
T
Miura
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003876:Dermatitis, Atopic / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000097:blood*; D008297:Male; D008875:Middle Aged
D015847:Interleukin-4