Rapid increases in cerebellar Purkinje cell glutamic acid decarboxylase (GAD67) mRNA after lesion-induced increases in cell firing.
Loss of the inferior olive-climbing fiber input to the cerebellar cortex doubles the simple spike activity of the cerebellar Purkinje cell. There is a 3- to 4-fold increase in Purkinje cell messenger RNA for the 67 kDa form of glutamic acid decarboxylase (a synthetic enzyme for the neurotransmitter GABA) within 4-5 h of the increase in electrical activity, suggesting a rapid response of mechanisms influencing neurotransmitter synthesis or stability to altered electrophysiological activity.
Drengler
S M
SM
Department of Pharmacology and Molecular Biology, University of Health Sciences, Chicago Medical School, IL 60064.
Oltmans
G A
GA
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D004594:Electrophysiology; D005968:Glutamate Decarboxylase / Q000096:biosynthesis*; D017403:In Situ Hybridization; D008297:Male; D011689:Purkinje Cells / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D005968:Glutamate Decarboxylase