Three monoclonal antibodies differentiate human from murine epidermis.
In order to establish a set of epidermal species markers, normal human skin, murine skin and human skin transplanted to nude mice were stained with monoclonal antibodies directed to cell membrane-bound carbohydrates, a basement membrane component and a structure in the cell nucleus. Three epidermal species markers were identified. Two markers stained exclusively human epidermis: LH7.2 detects type VII collagen and stained the basement membrane of human epidermis in unfixed frozen sections, while LP4N stained cell nuclei in human epidermis in methanol-acetone-fixed frozen sections. The third marker, HH14, stained exclusively murine epidermis. HH14 defines the histo-blood group H carbohydrate antigen and stained spinous cell membranes of murine epidermis in both frozen and formalin-fixed sections.
Jeppe-Jensen
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Department of Oral Diagnosis, Royal Dental College, Copenhagen, Denmark.
Clausen
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Leigh
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Lane
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Dabelsteen
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D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002240:Carbohydrate Sequence; D004817:Epidermis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D013045:Species Specificity
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D006017:Glycolipids; D006023:Glycoproteins