Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion.
Salmonella typhimurium and enteropathogenic Escherichia coli (EPEC) were found to adhere to the brush border of differentiated human intestinal epithelial Caco-2 cells in culture, whereas Yersinia pseudotuberculosis and Listeria monocytogenes adhered to the periphery of undifferentiated Caco-2 cells. All these enterovirulent strains invaded the Caco-2 cells. Using a heat-killed human Lactobacillus acidophilus (strain LB) which strongly adheres both to undifferentiated and differentiated Caco-2 cells, we have studied inhibition of cell association with and invasion within Caco-2 cells by enterovirulent bacteria. Living and heat-killed Lactobacillus acidophilus strain LB inhibited both cell association and invasion of Caco-2 cells by enterovirulent bacteria in a concentration-dependent manner. The mechanism of inhibition of both adhesion and invasion appears to be due to steric hindrance of human enterocytic pathogen receptors by whole-cell lactobacilli rather than to a specific blockade of receptors.
Coconnier
M H
MH
Département de Microbiologie et Immunologie, UFR de Sciences Pharmaceutiques Paris XI, Châtenay-Malabry, France.
Bernet
M F
MF
Kernéis
S
S
Chauvière
G
G
Fourniat
J
J
Servin
A L
AL
D001422:Bacterial Adhesion / Q000502:physiology*; D001667:Binding, Competitive; D002478:Cells, Cultured; D004755:Enterobacteriaceae / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology / Q000648:ultrastructure; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000382:microbiology* / Q000648:ultrastructure; D007779:Lactobacillus acidophilus / Q000502:physiology* / Q000648:ultrastructure; D008089:Listeria monocytogenes / Q000502:physiology* / Q000648:ultrastructure; D008871:Microvilli / Q000382:microbiology* / Q000648:ultrastructure; D012486:Salmonella typhimurium / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure; D015011:Yersinia pseudotuberculosis / Q000502:physiology / Q000648:ultrastructure