Detection of an adenylate cyclase gene in Pasteurella species.
A Pasteurella multocida adenylate cyclase gene has been previously cloned in Escherichia coli and sequenced. A 1200 bp HpaI fragment from the coding region was used as a probe to analyse the presence of the gene in different Pasteurella species and subspecies, Actinobacillus ureae (formerly P. ureae) and group EF-4 bacteria. Thirty-seven strains were checked for the presence of the gene. It was shown that the adenylate cyclase gene was detected only in the species Pasteurella multocida.
Escande
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F
Centre National de Référence des Pasteurella, Institut Pasteur, Paris, France.
Crasnier
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M
D000262:Adenylyl Cyclases / Q000235:genetics*; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D010325:Pasteurella / Q000201:enzymology / Q000235:genetics*; D016979:Pasteurella multocida / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D000262:Adenylyl Cyclases