Identification of the product of the murine ST2 gene.
The murine ST2 gene is expressed in growth-stimulated BALB/c-3T3 cells. This gene encodes a protein that is similar to the extracellular portions of the interleukin-1 receptors (types 1 and 2). In this study, we prepared a polyclonal antibody against the recombinant ST2 protein produced in Escherichia coli. This antibody detected recombinant ST2 protein in the culture fluid of COS7 cells transfected with a mammalian expression vector (pEF-BOS) carrying ST2 cDNA. Using this antibody, we could detect the ST2 protein in the culture fluid of growth-stimulated BALB/c-3T3 cells, and in the medium of continuously growing cells, but not in that of growth-arrested cells. ST2 proteins produced in COS7 cells and BALB/c-3T3 cells were N-glycosylated as predicted from nine putative N-glycosylation sites in its deduced amino-acid sequence.
Takagi
T
T
Department of Biochemistry II, Jichi Medical School, Tochigi, Japan.
Yanagisawa
K
K
Tsukamoto
T
T
Tetsuka
T
T
Nagata
S
S
Tominaga
S
S
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D004926:Escherichia coli; D015151:Immunoblotting; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D018123:Receptors, Interleukin; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis
C486189:Il1rl1 protein, mouse; D000072179:Interleukin-1 Receptor-Like 1 Protein; D008565:Membrane Proteins; D011506:Proteins; D018123:Receptors, Interleukin; D011994:Recombinant Proteins