title: A putative transcription factor binding to the upstream region of the puf operon in Rhodobacter sphaeroides.
abstract: Gel shift assays of the upstream region of the puf operon in Rhodobacter sphaeroides were performed using cell-free extracts from cells grown under various culture conditions. The results suggested that a protein binding to the upstream region functioned as a repressor-like substance of the expression of the operon by oxygen tension or light. The density of the shifted band of cell-free extracts from cells irradiated with blue light under semi-aerobic conditions was higher than that with red light. Phosphatase treatment of the cell-free extracts strongly increased the DNA-binding affinity of the protein.
authors.affiliation: Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shimada
authors.forename: H
authors.initials: H
authors.lastname: Ohta
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: Y
authors.initials: Y
authors.lastname: Shioi
authors.forename: K
authors.initials: K
authors.lastname: Takamiya
mesh_terms: D001483:Base Sequence; D002474:Cell-Free System; D015342:DNA Probes; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D008027:Light; D008969:Molecular Sequence Data; D009876:Operon; D012242:Rhodobacter sphaeroides / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors