[Study of the transplacental transfer of interferon alfa-2a on the model of isolated perfused cotyledon].
Interferon can be used for VIH+ pregnant women, to decrease materno-fetal contamination. Added to maternal circulation, its behaviour was studied by human placental cotyledon ex vivo perfusion. Human recombinant IFN 2a and reference substance 3H2O were injected in intervillous chamber and their behaviours in venous fetal and maternal circulations was followed. At steady state, in fetal circulation 3H2O concentration was 37% of injected rate whereas no IFN transfer rate was observed. In both venous circulations IFN amounts were lower than injected ones 56% versus of 82% for water (p < 0.05). IFN didn't cross placental filter and disappeared partially during placental contact.
Dumas
J C
JC
INSERM C.J.F. 89-08, CHU La Grave, Toulouse.
Giroux
M
M
Teixeira
M G
MG
Puel
J
J
Waysbort
A
A
Berrebi
A
A
Houin
G
G
Grandjean
H
H
D002824:Chorionic Villi / Q000378:metabolism*; D005260:Female; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000008:administration & dosage / Q000378:metabolism*; D008431:Maternal-Fetal Exchange; D010477:Perfusion / Q000379:methods; D011247:Pregnancy; D011994:Recombinant Proteins
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins
Etude du passage transplacentaire de l'interféron alpha 2a sur le modèle du cotylédon isolé perfusé.