title: Identification of a structural glycoprotein of an RNA virus as a ribonuclease.
abstract: One of the three structural glycoproteins of classical swine fever virus (CSFV) is E0, a disulfide-bonded homodimer that induces virus-neutralizing antibodies and occurs in a virion-bound as well as a secreted form. E0 was shown to be similar to a family of fungal and plant ribonucleases. Purified E0 from CSFV-infected cells was a potent ribonuclease specific for uridine and inhibitable by zinc ions.
authors.affiliation: Department of Biochemistry, Faculty of Natural Sciences, University of Innsbruck, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Schneider
authors.forename: G
authors.initials: G
authors.lastname: Unger
authors.forename: R
authors.initials: R
authors.lastname: Stark
authors.forename: E
authors.initials: E
authors.lastname: Schneider-Scherzer
authors.forename: H J
authors.initials: HJ
authors.lastname: Thiel
mesh_terms: D000595:Amino Acid Sequence; D006692:Classical Swine Fever Virus / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D004229:Dithiothreitol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012331:RNA, Fungal / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017421:Sequence Analysis; D057127:Single-Chain Antibodies; D013379:Substrate Specificity; D013696:Temperature; D014529:Uridine / Q000378:metabolism; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D012331:RNA, Fungal; D057127:Single-Chain Antibodies; D015678:Viral Structural Proteins; C082749:glycoprotein EO, classical swine fever virus; D012260:Ribonucleases; D004229:Dithiothreitol; D014529:Uridine