Biosynthesis of serine proteases in Lutzomyia anthophora (Diptera: Psychodidae).
Changes in the biosynthesis of serine proteases in adult Lutzomyia anthophora Addis were followed and compared with the larval and pupal stages. More chymotrypsinlike than trypsinlike enzyme was synthesized by 2-d-old and 3-d-old sugar-fed females and females that were fed blood 72 h earlier. A small increase in the amount of chymotrypsinlike enzyme occurred within the first 48 h after blood feeding, whereas trypsinlike enzyme activity increased rapidly after the blood meal and peaked at 72 h. [1,3-3H]DIP trypsinlike and chymotrypsinlike derivatives of sugar-fed and blood-fed females were compared using polyacrylamide gel electrophoresis.
Mahmood
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F
IFAS, University of Florida, Florida Medical Entomology Laboratory, Vero Beach 32962.
Borovsky
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D
D000818:Animals; D002918:Chymotrypsin / Q000096:biosynthesis; D004064:Digestive System / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005247:Feeding Behavior; D005260:Female; D007527:Isoenzymes / Q000096:biosynthesis*; D011576:Psychodidae / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D013997:Time Factors; D014357:Trypsin / Q000096:biosynthesis
D007527:Isoenzymes; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin