title: The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region.
abstract: A chimeric cDNA was constructed, coding for the N-terminal region of BS-RNase (residues 1-49) and the C-terminal region of RNase A (residues 50-124). The resulting chimeric DNA was expressed in Escherichia coli and found to code for RNase chains that spontaneously assembled into a covalently dimeric ribonuclease.
authors.affiliation: Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Di Donato
authors.forename: V
authors.initials: V
authors.lastname: Cafaro
authors.forename: M
authors.initials: M
authors.lastname: de Nigris
authors.forename: M
authors.initials: M
authors.lastname: Rizzo
authors.forename: G
authors.initials: G
authors.lastname: D'Alessio
mesh_terms: D000818:Animals; D002417:Cattle; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000235:genetics; D012260:Ribonucleases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D012661:Semen / Q000201:enzymology*
chemical_list: D011993:Recombinant Fusion Proteins; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic