title: Effect on single-breath washout and lung function of elastase-induced emphysema in rats.
abstract: Lung volumes, diffusing capacity (DLCO), quasi-static pressure-volume curves (P-V), forced expiration (FE) and He-SF6 single-breath washout (SBW) were performed in Wistar rats with emphysema induced by different doses of pancreatic elastase in saline, instilled intratracheally 6 wk prior to the tests. Emphysema was quantitatively assessed by mean linear intercept (Lm) measurements on 5-microns lung sections. Lung volume, P-V curve, and FE dependence on Lm, as well as the nonsignificant dependence of DLCO on Lm, are generally similar to results reported by others. The most interesting observation concerns the SBW: N2 slopes of the alveolar plateau, compared for identical lung volumes, did not change with the degree of emphysema. By contrast, the He-SF6 slope difference did depend significantly on the degree of emphysema. Based on the diffusion front theory, the present work suggests that in rats with elastase-induced emphysema, the phase III slope modifications relate mainly to elastic and not to structural alterations.
authors.affiliation: Laboratorio de Fisiopatología Respiratoria, Fundacion Jiménez Días, Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Gonzalez Mangado
authors.forename: G
authors.initials: G
authors.lastname: Peces-Barba
authors.forename: J J
authors.initials: JJ
authors.lastname: Cabanillas
authors.forename: G
authors.initials: G
authors.lastname: Renedo
authors.forename: S
authors.initials: S
authors.lastname: Verbanck
authors.forename: M
authors.initials: M
authors.lastname: Paiva
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001944:Breath Tests / Q000295:instrumentation / Q000379:methods; D016001:Confidence Intervals; D004195:Disease Models, Animal; D008168:Lung / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D010196:Pancreatic Elastase / Q000494:pharmacology*; D011656:Pulmonary Emphysema / Q000139:chemically induced* / Q000453:epidemiology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D012129:Respiratory Function Tests / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data
chemical_list: D010196:Pancreatic Elastase