Glycoprotein 2 of zymogen granule membranes shares immunological cross-reactivity and sequence similarity with phospholipase A2.
Glycoprotein 2 (GP2), the major protein of rat pancreatic zymogen granule membranes (ZGMs), cross-reacted with an antiserum against porcine secretory phospholipase A2 (sPLA2). Amino acid sequence comparison showed 45% similarity and 23% identity between porcine PLA2 and the C-terminal portion of GP2. An antiserum to intestinal brush border Ca(2+)-independent PLA2 (bbPLA2) also recognized GP2. The antigenic and sequence similarities between GP2, sPLA2, and bbPLA2 imply that the role of GP2 in cellular function is associated with phospholipid binding and/or hydrolysis.
Withiam-Leitch
M
M
Department of Pharmacology, State University of New York, Buffalo 14214.
Aletta
J M
JM
Koshlukova
S E
SE
Rupp
G
G
Beaudoin
A R
AR
Rubin
R P
RP
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003429:Cross Reactions; D003594:Cytoplasmic Granules / Q000276:immunology*; D058851:GPI-Linked Proteins; D007422:Intestines / Q000201:enzymology; D007425:Intracellular Membranes / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D008871:Microvilli / Q000201:enzymology; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology; D010741:Phospholipases A / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D013552:Swine
D058851:GPI-Linked Proteins; C066161:Gp2 protein, rat; D008562:Membrane Glycoproteins; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium