Ribosomal proteins, TL4 and TL5, from Thermus thermophilus form hybrid complexes with 5 S ribosomal RNA from different microorganisms.
Hybrid complexes of the ribosomal proteins, TL4 and TL5, from Thermus thermophilus with 5 S ribosomal RNA from Escherichia coli and Bacillus stearothermophilus have been prepared. There was no competition between the two proteins for the binding sites. Stoichiometry of 5 S RNA binding for both proteins was 1:1 (protein/RNA). The TL4 protein competed with the E. coli ribosomal L5 protein, and the TL5 protein competed with the E. coli ribosomal proteins, L18 and L25, for binding with 5 S RNA.
Gongadze
G M
GM
Department of Structure and Function of Ribosomes, Russian Academy of Sciences, Pushchino, Moscow Region.
Tishchenko
S V
SV
Sedelnikova
S E
SE
Garber
M B
MB
D004926:Escherichia coli / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D016963:Thermus thermophilus / Q000378:metabolism*
D012341:RNA, Ribosomal, 5S; D012269:Ribosomal Proteins