title: Cloning and sequence analysis of a cDNA encoding tetrameric carbonyl reductase of pig lung.
abstract: A cDNA for tetrameric carbonyl reductase was cloned from a pig lung cDNA library. The cDNA had an open reading frame encoding a protein of 244 amino acid residues with a predicted monomer molecular weight of 25,985. The authenticity of the cDNA was confirmed by a perfect match of 80 amino acids determined from sequencing of amino-terminus, carboxy-terminus and internal fragments of the purified enzyme. The deduced sequence of the carbonyl reductase shows a similarity to those of the short-chain alcohol dehydrogenase superfamily proteins; notably it had an extensive homology (85% identical residues) with a putative gene product of murine adipocyte.
authors.affiliation: Laboratory of Biochemistry, Gifu Pharmaceutical University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakanishi
authors.forename: Y
authors.initials: Y
authors.lastname: Deyashiki
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: A
authors.initials: A
authors.lastname: Hara
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D008168:Lung / Q000201:enzymology*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D011506:Proteins; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C082627:pig lung carbonyl reductase; C082628:Cbr2 protein, mouse