Azadirachtin inhibits proliferation of Sf 9 cells in monolayer culture.
Azadirachtin A in ppm quantity inhibits proliferation and monolayer formation of Spodoptera frugiperda (Sf9) insect cells in monolayer culture. The incubated cells demonstrate reduced rates of protein synthesis which finally leads to cell death. This growth inhibiting activity is compared with other botanicals such as ohchinin, salannin and volkensin. The high and specific activity of azadirachtin A on insect cells is discussed in comparison with its effect on a mammalian cell line, based on 2 D PAGE analysis of the total protein contents.
Rembold
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Max-Planck-Institut für Biochemie, Martinsried, Bundesrepublik Deutschland.
Annadurai
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RS
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins; D036701:Limonins; D015394:Molecular Structure; D009036:Moths; D009699:N-Glycosyl Hydrolases; D037121:Plant Lectins; D010940:Plant Proteins / Q000494:pharmacology; D014176:Protein Biosynthesis; D054790:Ribosome Inactivating Proteins, Type 2; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*
D006023:Glycoproteins; D036701:Limonins; D037121:Plant Lectins; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014315:Triterpenes; C049134:volkensin; D009699:N-Glycosyl Hydrolases; C010329:azadirachtin