title: The absence of Harvey ras mutations during development and progression of squamous cell carcinomas of the head and neck.
abstract: We have examined the incidence of Harvey ras mutations in human squamous cell carcinomas (SCC) of the upper aerodigestive tract using the polymerase chain reaction (PCR) followed by direct sequencing. No mutations were detected at codons 12, 13, 59 or 61 of this gene in any of six papillomas, five erythroplakias, 56 squamous cell carcinomas, and 16 SCC cell lines. Some of the SCC were lymph node metastases (three) or tumours which had recurred following radiotherapy (seven). We conclude that Harvey ras mutations are not a common event in the pathogenesis or recurrence of SCCs from Caucasian subjects, in contrast to the situation with Indian populations (Saranath et al., 1991).
authors.affiliation: Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow, U.K.
authors.forename: L J
authors.initials: LJ
authors.lastname: Clark
authors.forename: K
authors.initials: K
authors.lastname: Edington
authors.forename: I R
authors.initials: IR
authors.lastname: Swan
authors.forename: K A
authors.initials: KA
authors.lastname: McLay
authors.forename: W J
authors.initials: WJ
authors.lastname: Newlands
authors.forename: L C
authors.initials: LC
authors.lastname: Wills
authors.forename: H A
authors.initials: HA
authors.lastname: Young
authors.forename: P W
authors.initials: PW
authors.lastname: Johnston
authors.forename: R
authors.initials: R
authors.lastname: Mitchell
authors.forename: G
authors.initials: G
authors.lastname: Robertson
mesh_terms: D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D011905:Genes, ras; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D044465:White People