title: Electrospray mass spectrometric analysis of river buffalo (Bubalus bubalis) hemoglobins. Re-examination of alpha 1 and alpha 3 globin chain sequences.
abstract: 1. The accurate molecular weight of the globin chains from river buffalo hemoglobin components has been determined by means of electrospray mass spectrometry. 2. The ES/MS analysis demonstrated that all the buffalo Hb components share a common beta chain whose mass value coincides with that expected on the basis of the reported sequence. 3. The AA phenotype alpha 1 and alpha 3 globin chains exhibited a 30 Da mass difference as compared to their predicted molecular weights. Careful re-examination of the two alpha globin sequences by FAB/MS revealed the occurrence of sequence errors and hence the correct primary structure of both alpha chains was established.
authors.affiliation: Servizio di Spettrometria di Massa del CNR, Napoli, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Ferranti
authors.forename: A
authors.initials: A
authors.lastname: Di Luccia
authors.forename: A
authors.initials: A
authors.lastname: Malorni
authors.forename: L
authors.initials: L
authors.lastname: Ferrara
authors.forename: G
authors.initials: G
authors.lastname: Marino
authors.forename: P
authors.initials: P
authors.lastname: Pucci
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002020:Buffaloes / Q000097:blood*; D005914:Globins / Q000737:chemistry* / Q000235:genetics; D006454:Hemoglobins / Q000737:chemistry* / Q000235:genetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010641:Phenotype; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D006454:Hemoglobins; D005914:Globins