TRP-1 expression correlates with eumelanogenesis in human pigment cells in culture.
We have investigated the relationship in human cultured normal and malignant melanocytes between the accumulation of mRNAs encoding tyrosinase and tyrosinase-related protein-1 (TRP-1), the activity of tyrosinase and the presence of melanin. Tyrosinase mRNA correlates with tyrosinase activity and with the presence of pheomelanin, eumelanin or both melanin types. In contrast TRP-1 mRNA is only detectable in cells containing eumelanin, which suggests a role for TRP-1 in the eumelanin synthesis pathway.
del Marmol
V
V
LOCE-Faculty of Medicine, Université Libre de Bruxelles, Belgium.
Ito
S
S
Jackson
I
I
Vachtenheim
J
J
Berr
P
P
Ghanem
G
G
Morandini
R
R
Wakamatsu
K
K
Huez
G
G
D001483:Base Sequence; D002478:Cells, Cultured; D004277:DNA, Single-Stranded; D006801:Humans; D008543:Melanins / Q000096:biosynthesis* / Q000378:metabolism; D008544:Melanocytes / Q000201:enzymology / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004277:DNA, Single-Stranded; D008543:Melanins; D008562:Membrane Glycoproteins; D011506:Proteins; D012333:RNA, Messenger; C018362:pheomelanin; C041877:eumelanin; D010088:Oxidoreductases; C489799:TYRP1 protein, human; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase