title: Measurement of ascorbic acid in human aqueous humour and plasma and bovine aqueous humour by high-performance liquid chromatography with electrochemical detection.
abstract: High-performance liquid chromatography (HPLC) on ODS silica with cetyltrimethylammonium bromide as an ion-pairing reagent and electrochemical detection (ECD) was used to determine ascorbic acid in bovine and human aqueous humour and human plasma. Hydroquinone was used as the internal standard. A calibration curve plotted with ascorbic acid concentrations in the range 0.5-5 micrograms ml-1 for peak height versus internal standard peak height had a correlation coefficient of 0.998. The RSD (precision) between analyses of the same diluted sample was 1.5% and the RSD (reproducibility) between analyses of separate aliquots of the same sample of aqueous humour was 1.6%.
authors.affiliation: Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
authors.forename: D G
authors.initials: DG
authors.lastname: Watson
authors.forename: Z
authors.initials: Z
authors.lastname: Iqbal
authors.forename: J M
authors.initials: JM
authors.lastname: Midgley
authors.forename: H
authors.initials: H
authors.lastname: Pryce-Jones
authors.forename: L
authors.initials: L
authors.lastname: Morrison
authors.forename: G N
authors.initials: GN
authors.lastname: Dutton
authors.forename: S
authors.initials: S
authors.lastname: Karditsas
authors.forename: W
authors.initials: W
authors.lastname: Wilson
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D001205:Ascorbic Acid / Q000032:analysis* / Q000097:blood; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents
chemical_list: D007202:Indicators and Reagents; D001205:Ascorbic Acid