[The characteristics of monoclonal antibodies and their antigens associated with human sperm acrosome reaction. I. The induction of acrosome reaction and monoclonal antibody production].
Sperm released from the male genital tract must undergo capacitation and acrosome reaction (AR) before binding to oocyte membrane. Changes of sperm components have been found after the capacitation and AR. Based on these changes, the new contraceptive methods and the treatment of the male infertility might be approached. In the present study, in vitro capacitated human sperm were induced to undergo AR in BWW-BSA medium with calcium ionophore A 23187. The fresh human AR sperm in about 50% of sperm population assayed by triple stain technique and/or chlortetracycline fluorescence staining were used for immunization. Twenty three murine hybridomas secreting monoclonal antibodies (McAbs) to non-treated (NT) and/or AR human sperm have been obtained. Of them, 21 McAbs were classified as IgM, others as IgG1 and IgG2a. Based on the immunoreactions of 23 McAbs with NT and AR sperm, they were divided into three groups: group A reacted mainly with the AR sperm, group B with NT sperm, and group C with both AR and NT sperm. The cross reaction of these McAbs with human leukemia cell lines was detected by ELISA and the discrepant reactions were observed.
Wang
Y M
YM
Kunming Institute of Zoology, Academia Sinica, Kunming.
Ben
K L
KL
Cao
X M
XM
D000177:Acrosome / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000001:Calcimycin; D003429:Cross Reactions; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007075:Immunoglobulin M / Q000096:biosynthesis; D066298:In Vitro Techniques; D007938:Leukemia / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D000001:Calcimycin