Spermatid nucleus of Megaselia scalaris Loew (Insecta, Diptera, Phoridae): a study using anti-histone antibodies, scanning electron microscopy, and a centromere-specific oligonucleotide.
The structure of elongated spermatid nuclei was examined in the fly Megaselia scalaris using indirect immunofluorescence with anti-histone antibodies, scanning electron microscopy, and in situ hybridization with a centromere-specific oligonucleotide. The immunofluorescence experiments showed that, in keeping with the situation in most animals, histones are hyperacetylated prior to their displacement from the nucleus in the course of spermiogenesis. Scanning electron microscopy revealed that grooves run parallel to the long axis of the spermatid nuclei. The chromatin is segmented into blocks lateral to the grooves. This finding most probably indicates that the chromatin is not yet maximally condensed at this stage. The retarded chromatin condensation may be correlated with the export of somatic histones from the nucleus. The location of the centromeres could not be identified using scanning electron microscopy, but in situ hybridization showed that a centromere-specific oligonucleotide mapped to the central or close to the central areas within the spermatid nucleus. Possibly, the chromosomes are extended and arranged parallel to the long axis of the spermatid nucleus.
Wolf
K W
KW
Institut für Biologie der Medizinischen Universität zu Lübeck, Germany.
Jeppesen
P
P
Mitchell
A
A
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002503:Centromere / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D004175:Diptera / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D006657:Histones / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D013087:Spermatids / Q000378:metabolism / Q000648:ultrastructure*
D006657:Histones; D015345:Oligonucleotide Probes; D004247:DNA