Suppression of ras-mediated transformation. Differential expression of genes encoding extracellular matrix proteins in normal, transformed and revertant cells.
A subtraction hybridization technique was used to identify genes specifically expressed in phenotypic revertants derived from cells transformed by the H-ras oncogene. The expression of genes coding for components of the extracellular matrix appears to be frequently down-regulated in transformed cells. Partial restoration is associated with the reexpression of the normal phenotype in revertants.
Hajnal
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A
Department of Pathology, University of Zürich, Switzerland.
Klemenz
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R
Schäfer
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R
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D003094:Collagen / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015536:Down-Regulation; D005786:Gene Expression Regulation; D011905:Genes, ras; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010641:Phenotype; D011347:Procollagen / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D016324:Sequence Tagged Sites
D011347:Procollagen; D003094:Collagen; D004247:DNA