title: The nucleotide sequence of human aminoacylase-1.
abstract: The nucleotide sequence of a cDNA coding for human aminoacylase-1 (N-acylamino acid aminohydrolase, ACY-1, EC 3.5.1.14) subunit has been determined. The amino acid sequence of human ACY-1 subunit deduced from its cDNA nucleotide sequence showed a high degree of identity (87.7%) with the corresponding protein from porcine [1], although the former is one amino acid residue longer than the latter. Of 12 histidine and 3 cysteine residues, conserved between the proteins from two species, some are anticipated to form the active site of ACY-1 as either catalytic residues or ligands for an essential Zn2+ atom.
authors.affiliation: Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Otsu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Mitta
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Tsunasawa
mesh_terms: D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D004247:DNA; D000581:Amidohydrolases; C019672:aminoacylase I