Preferential hybridization: amplification of hybridization signals with nested probes.
This paper describes a protocol for the amplification of hybridization signals using single-stranded, digoxigenin-labeled probes of increasing length. Probes of different lengths were produced by a unidirectional polymerase chain reaction. Labeled probes were purified and collectively applied for the detection of immobilized nucleic acids. The method provides for a higher signal intensity than that observed with probes that cover the entire length of the target sequence. An increase of up to 4-fold in the intensity of the detection signal was observed.
Alsaadi
S A
SA
Department of Biochemistry, University of Leicester, UK.
D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D004076:Digoxigenin; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009975:Orthomyxoviridae / Q000235:genetics; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004279:DNA, Viral; D004076:Digoxigenin