title: Determination of ampicillin in human plasma by high-performance liquid chromatography using ultraviolet detection.
abstract: A high-performance liquid chromatographic method with isocratic elution has been developed for the determination of ampicillin in human plasma. The method comprises injection of the plasma after protein precipitation on to a reversed-phase C18 column, using phosphate buffer (pH3.5)-methanol as the mobile phase. The method involves UV detection at 225 nm. This method provides a simple technique for the rapid analysis of ampicillin within the concentration range 0.2-25 micrograms ml-1 with a high degree of accuracy and precision.
authors.affiliation: Research and Development Laboratories, Wellcome Pakistan Limited, Karachi.
authors.forename: M J
authors.initials: MJ
authors.lastname: Akhtar
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
mesh_terms: D000667:Ampicillin / Q000097:blood*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002021:Buffers; D007202:Indicators and Reagents; D000667:Ampicillin