title: Transfusion-transmitted Yersinia enterocolitica infection. Protection through buffy coat removal and failure of the bacteria to grow in platelet-rich or platelet-poor plasma.
abstract: In a previous study, removal of white blood cells (WBC), by filtration 5 h after deliberate contamination of whole blood with a type 0:3 strain of Yersinia enterocolitica, was shown to be an effective way of avoiding bacterial growth in red blood cells (RBC) during storage. In the present study the Opti-System technique was used to remove the buffy coat from whole blood, leaving 10-20% of the original number of WBC in the RBC preparation. In one series of experiments, all of 4 units of RBC suspension, from which buffy coats were removed 2 h after inoculation of 112 colony-forming units (cfu) per ml of Y. enterocolitica, became Yersinia-free, while abundant bacterial growth occurred in all of 4 units where RBC suspension and buffy coat had been recombined. In a second series of 10 experiments, with an inoculum of 80 cfu/ml, no growth was found in platelet-poor plasma stored for 42 days at 4 degrees C. Five out of 10 RBC suspensions in SAGM additive solution remained Yersinia-free throughout a 6-week storage period; 4 of these 10 units showing growth of Yersinia after 4 weeks and 5 after 6 weeks. In the buffy coats bacterial growth was found in 1 out of 10 units after 1 week, 4 after 2 weeks, and in all of 10 units after 4 weeks. In 2 control experiments with WBC-reduced RBC inoculated with the same bacterial dose, growth started within 24 h and was abundant after 1 week.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Clinical Immunology, University Hospital, Uppsala, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Gong
authors.forename: C F
authors.initials: CF
authors.lastname: Högman
authors.forename: A
authors.initials: A
authors.lastname: Hambraeus
authors.forename: C S
authors.initials: CS
authors.lastname: Johansson
authors.forename: L
authors.initials: L
authors.lastname: Eriksson
mesh_terms: D006801:Humans; D007958:Leukocyte Count; D010976:Platelet Count; D065227:Transfusion Reaction; D015009:Yersinia Infections / Q000517:prevention & control / Q000635:transmission*; D015008:Yersinia enterocolitica / Q000254:growth & development*