Prednisolone enhances myogenesis and dystrophin-related protein in skeletal muscle cell cultures from mdx mouse.
The differentiation of skeletal muscle cells from mdx mice which lack dystrophin expression was examined after glucocorticoid treatment, namely alpha-methylprednisolone (PDN). Primary skeletal muscle cell cultures were established from newborn mdx, congenic C57BL/10, and allogenic BALB/C mice. We show that PDN promotes the myogenesis of both mdx- and control mice-derived cultures as determined by 1) the number of myotubes, 2) acetylcholine receptors, and 3) dystrophin and dystrophin-related protein levels. These results support the hypothesis that PDN could enhance the myogenesis of satellite cells and increase dystrophin-related protein expression in DMD treated patients.
Passaquin
A C
AC
Département d'Immunologie, Immunopharmacologie et Pathologie, Université Louis Pasteur, Illkirch, France.
Metzinger
L
L
Léger
J J
JJ
Warter
J M
JM
Poindron
P
P
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D016189:Dystrophin / Q000096:biosynthesis; D007150:Immunohistochemistry; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D008870:Microtubules / Q000187:drug effects; D024510:Muscle Development; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000378:metabolism / Q000473:pathology*; D011239:Prednisolone / Q000494:pharmacology*; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000378:metabolism; D049411:Utrophin
D003598:Cytoskeletal Proteins; D016189:Dystrophin; D008565:Membrane Proteins; D011950:Receptors, Cholinergic; C483600:Utrn protein, mouse; D049411:Utrophin; D011239:Prednisolone; D003402:Creatine Kinase