title: The roles of enteric bacterial sialidase, sialate O-acetyl esterase and glycosulfatase in the degradation of human colonic mucin.
abstract: Sialidase activity in normal faecal extracts showed a preference for mucin-related glycoprotein and oligosaccharide substrates, but the presence of two or more O-acetyl esters at positions C7-C9 on the sialic acids retarded the rate of hydrolysis. A specific sialate O-acetyl esterase was detected with a lower total activity relative to sialidase with mucin substrates and having a pH optimum of 7.8 and a KM of approximately 1 mM sialate O-acetyl ester. A specific glycosulfatase activity was found in faecal extracts using the substrate lactit-[3H]ol 6-O-sulfate with a pH optimum of pH 5.0 and a KM of approximately 1 mM. Faecal extracts from ulcerative colitis (UC) patients had higher sialate O-acetyl esterase and glycosulfatase activity, while mucin sialidase activity was unchanged. Metabolically labelled mucin isolated from UC patients contained less sulfate and had lower sialic acid O-acetylation compared with normal mucin. Colonic mucin was degraded more efficiently by faecal extracts from UC patients compared with normal extracts. The UC mucin was degraded more rapidly than the normal mucin by faecal enzyme extracts from both normal and UC subjects.
authors.affiliation: University Department of Medicine, Bristol Royal Infirmary, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Corfield
authors.forename: S A
authors.initials: SA
authors.lastname: Wagner
authors.forename: L J
authors.initials: LJ
authors.lastname: O'Donnell
authors.forename: P
authors.initials: P
authors.lastname: Durdey
authors.forename: R A
authors.initials: RA
authors.lastname: Mountford
authors.forename: J R
authors.initials: JR
authors.lastname: Clamp
mesh_terms: D000107:Acetylation; D000115:Acetylesterase; D002240:Carbohydrate Sequence; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000502:physiology*; D003093:Colitis, Ulcerative / Q000201:enzymology / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000201:enzymology*; D005243:Feces / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism*; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000502:physiology*; D012794:Sialic Acids / Q000032:analysis; D013429:Sulfatases / Q000737:chemistry / Q000502:physiology*
chemical_list: D009077:Mucins; D012794:Sialic Acids; C025676:9-O-acetyl-N-acetylneuraminic acid; D002265:Carboxylic Ester Hydrolases; D000115:Acetylesterase; C046298:sialate O-acetylesterase; D013429:Sulfatases; C022758:glycosulphatase; D009439:Neuraminidase