title: Analysis of lectin binding sites in the gut of hooded Lister rats with special emphasis on recently detected lectins.
abstract: Seven recently isolated lectins were tested for their ability to bind to tissue sections of rat gut. Binding sites for N-Acetylgalactosamine specific lectins were found in mucins, in the brush border membrane and in goblet cells. Non-reducing terminal mannose residues were absent from cell surface membranes but were detected in the supranuclear region of goblet cells and enterocytes. The results of lectin binding obtained in this study were generally similar to lectin-gut interactions observed in vivo.
authors.affiliation: University of Southampton, Bassett Crescent East, United Kingdom.
authors.forename: U
authors.initials: U
authors.lastname: Schumacher
authors.forename: H
authors.initials: H
authors.lastname: Madry
authors.forename: E
authors.initials: E
authors.lastname: Adam
authors.forename: W J
authors.initials: WJ
authors.lastname: Peumans
authors.forename: E J
authors.initials: EJ
authors.lastname: van Damme
authors.forename: G
authors.initials: G
authors.lastname: Grant
authors.forename: S
authors.initials: S
authors.lastname: Bardocz
authors.forename: A
authors.initials: A
authors.lastname: Pusztai
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D005690:Galactose / Q000032:analysis / Q000378:metabolism; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000378:metabolism; D007583:Jejunum / Q000737:chemistry* / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008358:Mannose / Q000032:analysis / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008871:Microvilli / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats
chemical_list: D037102:Lectins; D000116:Acetylgalactosamine; D008358:Mannose; D005690:Galactose