Regulation of the transcription factor c-JUN by nerve growth factor in adult sensory neurons.
We examined the regulation by nerve growth factor (NGF) of the immediate-early gene (proto-oncogene) c-jun in adult dorsal root ganglion (DRG) neurons using immunocytochemistry to c-JUN (the protein product of the proto-oncogene c-jun). Following a sciatic nerve crush, the injury-induced increase in c-JUN-like immunostaining was reduced in DRG neurons by continuous intrathecal infusion of NGF for one week. Conversely, in intact DRG neurons (i.e., without Wallerian degeneration), c-JUN-like immunoreactivity was markedly increased following four weeks of daily NGF antiserum injections (to remove target tissue-derived NGF) into the hindfoot. Taken together, these findings indicate that nerve transection (axotomy) results in a loss of target tissue-derived NGF leading to induction of the transcription factor c-jun which may play a role in axonal regeneration.
Gold
B G
BG
Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, Portland 97201-3098.
Storm-Dickerson
T
T
Austin
D R
DR
D000818:Animals; D001369:Axons / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D016758:Genes, jun; D006651:Histocytochemistry; D007278:Injections, Spinal; D008297:Male; D009409:Nerve Crush; D009414:Nerve Growth Factors / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000096:biosynthesis*
D009414:Nerve Growth Factors; D014157:Transcription Factors