Rapid primary microwave-glutaraldehyde fixation preserves the plasma membrane and intracellular structures of the protozoan Tritrichomonas foetus.
Tritrichomonas foetus, a pathogenic protozoan, was used as a model to analyse microwave-stimulated fixation as a procedure of preparation of biological samples for electron microscopy of thin sections and freeze-fracture replicas. Good preservation of the protozoan structure was achieved by microwave-stimulated fixation and Epon polymerization. The membrane structure, as visualized in freeze-fracture replicas, was well preserved.
Benchimol
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Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Goncalves
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de Souza
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D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D005614:Freeze Fracturing; D005976:Glutaral; D008854:Microscopy, Electron / Q000379:methods; D008872:Microwaves; D016707:Tissue Fixation / Q000379:methods*; D014021:Tissue Preservation; D016835:Tritrichomonas foetus / Q000648:ultrastructure*
D005976:Glutaral