[Effect of an electromagnetic field on subtilisin from Bacillus subtilis 316 m].
The influence of electromagnetic field on the subtilisin of Bacillus subtilis strain 316 M preparation has been studied. It is the increase 30-60% of the proteolytic activity during 60-100 min treatment was observed. The effect of activation is stable for a month at +4 degrees C. The increase of the proteolytic activity is connected with conformational changes of the enzyme molecule, with the decrease of pI from 11.4 to 9.2 in particular.
Povaliaeva
I V
IV
Koltukova
N V
NV
Kudria
V A
VA
D001412:Bacillus subtilis / Q000201:enzymology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004574:Electromagnetic Fields; D004789:Enzyme Activation / Q000528:radiation effects; D004795:Enzyme Stability / Q000528:radiation effects; D011487:Protein Conformation; D013381:Subtilisins / Q000737:chemistry / Q000528:radiation effects*
D013381:Subtilisins
Vozdeĭstvie élektromagnitnogo polia na subtilizin Bacillus subtilis 316 m.