Functional-morphological effect of the synthetic atrial natriuretic factor (ANF 101-126) on the zona glomerulosa cells of adrenal cortex.
The effects of atrial natriuretic factor (ANF) on the adrenal steroidogenesis were examined in the zona glomerulosa of rats in vivo. ANF administration provoked a significant decrease in the width of zona glomerulosa (P < 0.01) and a significant enlargement of nuclei of zona glomerulosa cells (P < 0.01). Ultrastructurally conspicuous change was observed in mitochondria of the cells of the zona glomerulosa treated with ANF, representing elongated mitochondria with "parallel arrays" of tubules with pipe-like structure. In vitro autoradiography 125I-labelled ANF binding displayed a significant decrease of a specific receptor site in the zona glomerulosa treated with ANF. A significant lowering in plasma levels of aldosterone was observed in ANF-treated rats. These findings could be interpreted as an inhibitory effect of ANF on adrenal steroidogenesis of the glomerular zone by action through a specific ANF-receptor.
Kawai
K
K
Department of Pathology, Nagasaki University School of Medicine, Japan.
Shigematsu
K
K
Nakatani
A
A
Irie
J
J
Kamio
T
T
Tsuchiyama
H
H
D000450:Aldosterone / Q000097:blood; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000494:pharmacology*; D003345:Corticosterone / Q000097:blood; D008297:Male; D008854:Microscopy, Electron; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015384:Zona Glomerulosa / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure
D010446:Peptide Fragments; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; C043970:atrial natriuretic factor 26, rat; D003345:Corticosterone