title: An elongated form of T4 glutaredoxin with four extra residues.
abstract: Two different forms of T4 glutaredoxin (thioredoxin) arising from the same gene on a multicopy plasmid in an Escherichia coli expression system have been isolated and characterized. Up to one-fourth of purified T4 glutaredoxin has an extension of four amino acids in the carboxy terminus, with the sequence aspartate, arginine, isoleucine, lysine. This four-residue extension may be caused by a translational +1 frameshift at the UGA terminator codon.
authors.affiliation: Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: M
authors.initials: M
authors.lastname: Nikkola
authors.forename: A
authors.initials: A
authors.lastname: Engström
authors.forename: M
authors.initials: M
authors.lastname: Saarinen
authors.forename: M
authors.initials: M
authors.lastname: Ingelman
authors.forename: T
authors.initials: T
authors.lastname: Joelson
authors.forename: H
authors.initials: H
authors.lastname: Eklund
mesh_terms: D000595:Amino Acid Sequence; D017122:Bacteriophage T4 / Q000235:genetics; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D054477:Glutaredoxins; D008969:Molecular Sequence Data; D010088:Oxidoreductases; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D054477:Glutaredoxins; D011506:Proteins; D010088:Oxidoreductases