Interleukin-6 and tumour necrosis factor alpha synergistically block S-phase cell cycle and upregulate intercellular adhesion molecule-1 expression on MCF7 breast carcinoma cells.
The effect of IL-6 and TNF alpha were studied on human MCF7 breast cancer cells. Synergistic interaction between IL-6 and TNF alpha, on the growth inhibition (50% reduction in the percentage of S-phase cells) and the upregulation of ICAM-1 expression (4 to 11-fold increase) was shown using flow cytometric methods. IL-6 and TNF alpha alone had negligible effect on the cell cycle. The individual effect of IL-6 resulted in down-regulation of ICAM-1 expression (30-35%), while TNF alpha always upregulated ICAM-1 (1.5 to 4-fold increase). The combined effect of IL-6 and TNF alpha consistently caused an increased expression of ICAM-1, which was greater than the sum of each one alone and also sustained for 72 h following cytokine withdrawal.
Bajaj
P
P
Institute for Cancer Studies, University of Sheffield Medical School, UK.
Lawry
J
J
Shenton
G
G
Rees
R C
RC
D001943:Breast Neoplasms; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D004357:Drug Synergism; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D015815:Cell Adhesion Molecules; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha