Cytotoxic effect of hemolytic culture supernatant from Enterococcus faecalis on mouse polymorphonuclear neutrophils and macrophages.
We reconfirmed that the LD50s of hemolytic Enterococcus faecalis strains were significantly less than those of nonhemolytic E. faecalis strains in normal mice. Hemolysin produced by E. faecalis lysed human, horse, rabbit, and mouse erythrocytes, but not cow and sheep erythrocytes. Sphingomyelin comprises a part of the lipid composition of the erythrocyte membrane of all mammalian species tested. But phosphatidylcholine exists only in human, horse, rabbit, and mouse. These two lipids inhibited lysis of horse erythrocytes by hemolytic E. faecalis. Phosphatidylcholine is probably the binding component on the membrane of erythrocytes for E. faecalis hemolysin. The hemolytic culture supernatant lysed not only erythrocytes but also mouse polymorphonuclear neutrophils (PMNs) and macrophages.
Miyazaki
S
S
Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
Ohno
A
A
Kobayashi
I
I
Uji
T
T
Yamaguchi
K
K
Goto
S
S
D000818:Animals; D002784:Cholesterol / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000276:immunology / Q000472:pathogenicity*; D016908:Gram-Positive Bacterial Infections / Q000276:immunology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D007928:Lethal Dose 50; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000276:immunology*; D010743:Phospholipids / Q000494:pharmacology; D013045:Species Specificity; D014774:Virulence
D006460:Hemolysin Proteins; D010743:Phospholipids; D002784:Cholesterol