title: Quantitation of HTLV-I provirus among seropositive blood donors: relation with antibody profile using synthetic peptides.
abstract: To study the pathophysiological state of HTLV-I carriers and the quantitative aspect of the risk of HTLV-I infection, we measured the absolute amounts of HTLV-I provirus in 39 seropositive blood donors. The amount of provirus varied from less than one to more than 10(4) molecules per 10(5) peripheral-blood mononuclear cells (PBMC). The average amount of HTLV-I provirus among seropositive blood donors was estimated as 3.7 x 10(3) molecules per 10(5) PBMC. The titer of the antibody against only the synthetic peptide (aa 100 to 130) of the gag p19 region of the HTLV-I provirus showed a weak but significant correlation with the amount of the provirus.
authors.affiliation: Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsumura
authors.forename: S
authors.initials: S
authors.lastname: Kushida
authors.forename: Y
authors.initials: Y
authors.lastname: Ami
authors.forename: T
authors.initials: T
authors.lastname: Suga
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: T
authors.initials: T
authors.lastname: Kameyama
authors.forename: A
authors.initials: A
authors.lastname: Terano
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Shiraki
authors.forename: K
authors.initials: K
authors.lastname: Okochi
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001782:Blood Donors; D002353:Carrier State / Q000276:immunology / Q000382:microbiology; D005260:Female; D015481:HTLV-I Antibodies / Q000097:blood*; D015490:HTLV-I Infections / Q000276:immunology / Q000382:microbiology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011533:Proviruses / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D015481:HTLV-I Antibodies