title: The sequence of hepatitis E virus isolated directly from a single source during an outbreak in China.
abstract: In this study an IgM antibody-mediated antigen-capture procedure for direct extraction of hepatitis E virus (HEV) RNA from clinical specimens was developed and used with an efficient method for generating viral cDNA that was subsequently sequenced using the dideoxy chain termination method. This is the first time the complete HEV genome has been isolated directly from a single human clinical specimen obtained during an outbreak of enterically transmitted non-A, non-B hepatitis. When the Chinese-derived sequence was compared with the original isolate of Burmese HEV from an experimentally infected cynomolgus macaque, the homology between the two sequences was 94% and 98.5% at the nucleotide and amino acid levels, respectively. The methods we developed for generating and sequencing genomic HEV cDNA dramatically improved the efficiency of cloning the viral genome and should be helpful for continued analysis of this virus as well as other RNA viruses that have proven to be difficult to clone and sequence directly.
authors.affiliation: Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333.
authors.forename: S L
authors.initials: SL
authors.lastname: Bi
authors.forename: M A
authors.initials: MA
authors.lastname: Purdy
authors.forename: K A
authors.initials: KA
authors.lastname: McCaustland
authors.forename: H S
authors.initials: HS
authors.lastname: Margolis
authors.forename: D W
authors.initials: DW
authors.lastname: Bradley
mesh_terms: D001483:Base Sequence; D002681:China / Q000453:epidemiology; D004279:DNA, Viral; D004196:Disease Outbreaks; D016679:Genome, Viral; D016751:Hepatitis E / Q000453:epidemiology* / Q000382:microbiology; D016752:Hepatitis E virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral