Purification of superoxide dismutase from placental haemolisate blood: a simple and efficient method.
Superoxide dismutase functions as a scavenger of superoxide radical protecting living organisms. This enzyme has potential use as anti-inflammatory or anti-reperfusion injury drug. Here we present a simple and efficient SOD purification method from human placental blood. Superoxide dismutase from clarified haemolysed placental blood after chloroform and ethanol treatment was purified by DEAE-Sepharose, Phenyl-Sepharose chromatographies and cross flow ultrafiltration. The purified product is 98% pure by SDS-PAGE with 71% yield and specific activity of 2.8 x 10(5) U/mg protein.
Bettarello
Y M
YM
Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil.
Cabrera-Crespo
J
J
Ho
P L
PL
Carvalho
A
A
Raw
I
I
D015153:Blotting, Western; D002725:Chloroform; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol; D006461:Hemolysis; D006801:Humans; D010920:Placenta / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000097:blood / Q000302:isolation & purification*; D014462:Ultrafiltration
D000431:Ethanol; D002725:Chloroform; D013482:Superoxide Dismutase