Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-gamma in a human colon cancer cell line: requirement for active metabolism and intact cytoskeleton.
The homotypic cell aggregation of a carcinoembryonic antigen (CEA) positive colon cancer cell line (Colo 205) was induced in vitro by interferon-gamma (IFN-gamma) treatment. Divalent cations were required for this aggregation, as it was inhibited by EDTA. The partial inhibition by cytochalasin B and the complete inhibition by a mixture of sodium azide and 2-deoxyglucose suggests that the aggregation requires the integrity of cytoskeleton and active metabolism. The expression of CEA was increased in the cytoplasm and on the membrane of Colo 205 by IFN-gamma treatment. Furthermore, this aggregation was inhibited completely by anti-CEA monoclonal antibody (mAb) and partially by mAb against intercellular adhesion molecule-1. This in vitro study suggests that CEA molecule participates in the IFN-gamma induced homotypic adhesion of some CEA positive cancer cells and that IFN-gamma has an important role in the regulation of cell-cell interaction mediated by CEA molecule.
Kanai
T
T
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Hibi
T
T
Hayashi
A
A
Takashima
J
J
Shiozawa
M
M
Aiso
S
S
Toda
K
K
Iwao
Y
Y
Watanabe
M
M
Tsuchiya
M
M
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000276:immunology / Q000502:physiology*; D002449:Cell Aggregation / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009362:Neoplasm Metastasis; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007371:Interferon-gamma