[Expression of the gene for transforming growth factor type alpha, transferred into A431 cells by a recombinant retroviral vector].
Cells of human epidermoid carcinoma A431 were used for obtaining of the cell line constantly expressing TGF-alpha. Recombinant virions were obtained by introducing the proviral DNA into PA317 cells by means of electrotransfection. A protein with the EGF-competing activity was found in a conditioned media of the chosen clone A431/1522-4. The concentration of this protein was several times higher than in a conditioned media of wild type A431 cells. By means of electrophoresis, isoelectric focusing, and immunochemical analysis it was shown that the protein is TGF-alpha. Similarly to EGF, the extracted TGF-alpha entirely displaced 125I-EGF specifically bound to receptor. TGF-alpha produced by the A431/1522 cells also stimulated autophosphorylation of the EGF receptor.
Pogrebnoĭ
P V
PV
Serov
S M
SM
Klenchin
V A
VA
Luk'ianov
A V
AV
Garmanchuk
L V
LV
Tarnavskiĭ
D V
DV
Markeeva
N V
NV
Bykorez
A I
AI
D001345:Autoradiography; D015139:Blotting, Southern; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing; D012190:Retroviridae / Q000235:genetics*; D016211:Transforming Growth Factor alpha / Q000032:analysis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D007457:Iodine Radioisotopes; D016211:Transforming Growth Factor alpha
Ekspressiia gena transformiruiushchego faktora rosta tipa alpha, perenesennogo rekombinantnym retrovirusnym vektorom v kletki A431.