Is an activator protein-2-like transcription factor involved in regulating gene expression during nitrogen limitation in fungi?
The upstream sequences of all published lignin peroxidase and manganese peroxidase genomic clones from Phanerochaete chrysosporium were analyzed. This analysis revealed the presence of putative activator protein-2 (AP-2) recognition sequences in 11 of 15 lignin peroxidase genes. The lignin peroxidase clone GLG6 and the manganese peroxidase gene (mnp-1) have two copies of putative AP-2 sequence in the upstream region. Interestingly, the lignin peroxidase gene VLG4 of another white rot fungus, Trametes versicolor, and the nit-2 gene of Neurospora crassa also contain putative AP-2-binding sequences. Since all of these genes are regulated by nutrient nitrogen, I hypothesize that an AP-2-like transcription factor may be involved in inducing gene expression during nitrogen limitation in fungi.
Dhawale
S S
SS
Indiana University Purdue University, Fort Wayne, Indiana 46805.
D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000172:deficiency; D010544:Peroxidases / Q000235:genetics*; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D009584:Nitrogen