Partial purification of a renotropic activity from plasma of uninephrectomized human subjects.
A renotropic factor was partially purified by sequential gel filtration and anion exchanger chromatography from plasma of human kidney transplantation donors and a renal cancer patient after uninephrectomy. This activity increased the rate of [3H]thymidine incorporation into DNA in rat cortical tubules, but not in rat liver cells, within the range of 100-200 ng/ml protein. The renotropic activity was detected between 7 and 12 days after uninephrectomy, and at least in 1 case decreased thereafter. This activity was undetected in gel-filtrated plasma of patients after a nonurological surgical procedure. The potency of this renotropic activity and its elution by gel filtration are similar to those displayed by a renal growth factor activity isolated from uninephrectomized rat plasma, as recently reported.
García-Ocaña
A
A
Metabolic Unit Research Laboratory, Fundación Jiménez-Díaz, Madrid, Spain.
Ortega
J
J
González-García
Y
Y
García-Cantón
C
C
Esbrit
P
P
D000328:Adult; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006133:Growth Substances / Q000097:blood*; D006801:Humans; D006965:Hyperplasia / Q000097:blood; D006984:Hypertrophy / Q000097:blood; D007668:Kidney / Q000254:growth & development* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009392:Nephrectomy
D006133:Growth Substances; D004247:DNA