Mammalian calponin. Identification and expression of genetic variants.
Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in some way in the regulation or modulation of contraction. Here we describe the cloning and bacterial expression of two calponin species from murine and porcine smooth muscle tissues. Primary and secondary structural analyses of the deduced amino acid sequences revealed a high degree of homology to avian calponin with the exception of a short and variable C-terminal segment. The sequence data demonstrate that the two mammalian calponin variants do not arise via alternative splicing but are encoded by different genes.
Strasser
P
P
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
Gimona
M
M
Moessler
H
H
Herzog
M
M
Small
J V
JV
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D002645:Chickens; D004247:DNA; D015180:Electrophoresis, Gel, Two-Dimensional; D014644:Genetic Variation; D051379:Mice; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics*; D009130:Muscle, Smooth / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013552:Swine; D000096985:Calponins
D002135:Calcium-Binding Proteins; D004247:DNA; D008840:Microfilament Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D000096985:Calponins