Investigation of aneuploidy induction in mouse oocytes following exposure to vinblastine-sulfate, pyrimethamine, diethylstilbestrol diphosphate, or chloral hydrate.
The various causative and mechanistic phenomena associated with aneuploidy induction require considerable investigation to better understand the etiology of chromosome missegregation. We investigated the potential of vinblastine sulfate, pyrimethamine, diethylstilbestrol diphosphate, and chloral hydrate to induce numerical and structural chromosome changes in female mouse germ cells. Superovulated ICR mice were administered the compounds either by intraperitoneal injection or oral gavage, and oocytes were collected and processed for cytogenetic analysis 17 hr later. Vinblastine sulfate, administered i.p., induced a significant increase in the frequency of ovulated MI oocytes and of hyperploid MII oocytes compared to controls, but did not increase the frequency of structural aberrations. Pyrimethamine, diethylstilbestrol diphosphate, and chloral hydrate did not increase the frequency of numerical or structural chromosome changes in female mouse germ cells.
Mailhes
J B
JB
Department of Obstetrics and Gynecology, Louisiana State University Medical Center, Shreveport 71130.
Aardema
M J
MJ
Marchetti
F
F
D000782:Aneuploidy; D000818:Animals; D002697:Chloral Hydrate / Q000633:toxicity*; D004054:Diethylstilbestrol / Q000031:analogs & derivatives* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005260:Female; D008677:Metaphase; D051379:Mice; D008813:Mice, Inbred ICR; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011739:Pyrimethamine / Q000633:toxicity*; D014747:Vinblastine / Q000633:toxicity*
D009153:Mutagens; D002697:Chloral Hydrate; D014747:Vinblastine; D004054:Diethylstilbestrol; C004955:fosfestrol; D011739:Pyrimethamine