Study on the purification of growth hormone-like substance from pituitaries of the snake Ptyas mucosa.
Extract from the snake (Ptyas mucosa) pituitaries was capable of inhibiting the binding of 125I-labelled bovine growth hormone to female rat liver membranes. The growth hormone-like substance was not adsorbed on Concanavalin A-Sepharose nor DEAE-cellulose, but could be purified by gel filtration on Sephadex G-50. It possessed a molecular weight of about 19,000 as judged by SDS-polyacrylamide gel electrophoresis. The iodinated snake growth hormone-like substance bound to membranes prepared from female rat and pregnant rabbit livers. The binding could be inhibited by unlabelled snake growth hormone-like substance as well as by bovine growth hormone.
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Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T.
Lee
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HM
Cheng
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CH
Wong
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CC
D000818:Animals; D001667:Binding, Competitive; D004305:Dose-Response Relationship, Drug; D005260:Female; D013006:Growth Hormone / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D010902:Pituitary Gland / Q000737:chemistry*; D011247:Pregnancy; D011817:Rabbits; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D011986:Receptors, Somatotropin / Q000378:metabolism; D012911:Snakes; D014018:Tissue Distribution
D011986:Receptors, Somatotropin; D013006:Growth Hormone