title: Electrophoretic karyotyping of the lignin-degrading basidiomycete Phanerochaete chrysosporium.
abstract: Electrophoretic karyotyping of the two most widely studied strains of Phanerochaete chrysosporium, BKMF-1767 and ME-446, has been determined using transverse alternating field electrophoresis. The genomic DNA of BKMF-1767 was resolved into 10 chromosomes ranging in size from 1.8-5.0 Mb, amounting to a total genome size of about 29 Mb. The genomic DNA of strain ME-446, on the other hand, was resolved into 11 chromosomes, amounting to a total genome size of about 32 Mb. Lignin peroxidase genes have been localized to five chromosomes in strain BKMF-1767 and to four chromosomes in strain ME-446.
authors.affiliation: Department of Microbiology, Michigan State University, East Lansing 48824-1101.
authors.forename: T M
authors.initials: TM
authors.lastname: D'Souza
authors.forename: S B
authors.initials: SB
authors.lastname: Dass
authors.forename: A
authors.initials: A
authors.lastname: Rasooly
authors.forename: C A
authors.initials: CA
authors.lastname: Reddy
mesh_terms: D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D007527:Isoenzymes / Q000235:genetics*; D007621:Karyotyping / Q000379:methods*; D008031:Lignin / Q000378:metabolism*; D010544:Peroxidases / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D007527:Isoenzymes; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase