ABO blood grouping on dental tissue.
Twenty-five permanent teeth, including eight carious ones whose pulp cavities had been exposed, were used for this research 3-5 weeks after extraction. Phosphate-buffered saline (PBS, at pH 7.2) was employed to extract ABO blood group substance from tooth powder. ABO grouping was performed on blood-stained compresses from the extraction wound (as controls), tooth fragment, tooth powder, and cotton fibers immersed in PBS extract by absorption-elution (AE) technique and on the PBS extracts by the two-dimensional absorption-inhibition (2-D AI) technique. It was found that blood grouping in PBS extracts by 2-D AI yielded reliable results: no false positive results, and a high rate of correct grouping, (24/25), while blood grouping on other dental materials, such as tooth fragments, tooth powders, immersed fibers, by AE gave an unacceptable rate of false positive/negative results.
Xingzhi
X
X
Department of Forensic Medicine, Shanghai Medical University, China.
Ji
L
L
Hao
F
F
Ming
L
L
Zhuyao
L
L
D000017:ABO Blood-Group System; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005188:False Negative Reactions; D005189:False Positive Reactions; D005553:Forensic Dentistry; D006801:Humans; D014070:Tooth / Q000276:immunology*
D000017:ABO Blood-Group System