title: Comparative oxidative rates of methionine hydroxy analogue in rat and chicken kidney.
abstract: 1. L-2-HAOX A and L-2-HAOX B were purified from chicken and rat kidney with purification factors of 550 and 45, respectively. 2. It was established that both enzymes were tetrameric (M(r) = 169,000) and consisted of 40 kDa monomers. 3. While chicken kidney L-2-HAOX A is N-terminally blocked like spinach glycolate oxidase and chicken liver L-2-HAOX A, L-2-HOAX B begins with a Pro residue. 4. The kinetic parameters of L-HMB oxidation by L-2-HAOX A and B were determined. 5. The results are particularly interesting as regards L-HMB oxidation by L-2-HAOX B.
authors.affiliation: Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
authors.forename: L
authors.initials: L
authors.lastname: Dupuis
authors.forename: J
authors.initials: J
authors.lastname: De Caro
authors.forename: A
authors.initials: A
authors.lastname: Puigserver
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008333:Mandelic Acids / Q000378:metabolism; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology; D008970:Molecular Weight; D010084:Oxidation-Reduction; D051381:Rats; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D008333:Mandelic Acids; C008391:alpha-hydroxy-gamma-methylmercaptobutyric acid; D008715:Methionine; D000429:Alcohol Oxidoreductases; C021380:L-2-hydroxyacid oxidase; C037938:mandelic acid