title: Interaction of GTP-binding protein Gq with photoactivated rhodopsin in the photoreceptor membranes of crayfish.
abstract: Interaction of G-protein with photoactivated rhodopsin (Rh*) in crayfish photoreceptor membranes was investigated by immunoprecipitation using an antibody against rhodopsin. Two kinds of protein were co-precipitated with rhodopsin. One is an alpha subunit of class-q G-protein (42 kDa, CGq alpha) which showed light-induced, dose-dependent binding to rhodopsin, and the other is an actin-like protein (44 kDa) with light-independent binding. Most of the CGq alpha was available for binding to Rh* but was dissociated from Rh* in the presence of GTP gamma S. These findings demonstrate that, in the crayfish photoreceptor, a Gq class of G-protein is activated by Rh*.
authors.affiliation: Institute of Biology, Faculty of Education, Oita University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Terakita
authors.forename: T
authors.initials: T
authors.lastname: Hariyama
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukahara
authors.forename: Y
authors.initials: Y
authors.lastname: Katsukura
authors.forename: H
authors.initials: H
authors.lastname: Tashiro
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000378:metabolism*; D012243:Rhodopsin / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D017386:Sequence Homology, Amino Acid
chemical_list: D012243:Rhodopsin; D019204:GTP-Binding Proteins