title: The gene coding for the major sheath protein of Litomosoides carinii microfilariae, gp22, is transcribed in oocytes and embryonic cells.
abstract: The transcription and translation of the gene encoding gp22, a major constituent of the microfilarial sheath of the filarial parasite Litomosoides carinii were studied by in situ hybridisation and immunohistology. Transcription of the gp22 gene is confined to oocytes and embryos in the reproductive organs of adult female worms. It starts in oocytes in the rhachis zone, is maximal in multicellular embryos and decreases slowly as the microfilariae develop. Blood microfilariae lack the gp22 transcript. The gp22 gene product is first detectable in parasites recovered on day 32 post infection. Expression of gp22 begins in multicellular embryos in the uteri of mature female worms and can be detected in all further developed intrauterine stages. The gp22 gene product appears to be exported by the embryonic cells and becomes integrated into the sheath where it may contribute to the flexibility of the latter structure.
authors.affiliation: Institut für Parasitologie, Justus-Liebig-Universität, Giessen, Germany.
authors.forename: F J
authors.initials: FJ
authors.lastname: Conraths
authors.forename: B
authors.initials: B
authors.lastname: Schützle
authors.forename: G
authors.initials: G
authors.lastname: Schares
authors.forename: H
authors.initials: H
authors.lastname: Christ
authors.forename: G
authors.initials: G
authors.lastname: Hobom
authors.forename: H
authors.initials: H
authors.lastname: Zahner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D005370:Filarioidea / Q000235:genetics* / Q000378:metabolism; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D015801:Helminth Proteins; D012333:RNA, Messenger