title: Differential effect of interleukin-1 beta on Ia expression in astrocytes and microglia.
abstract: In an earlier study we demonstrated the inhibitory effect of interleukin-1 beta (IL-1 beta) on human leukocyte antigens (HLA) class II enhancement by interferon-gamma (IFN-gamma) in a human glioblastoma multiforme cell line. In this study we have examined the effect of IL-1 beta on IFN-gamma induced major histocompatibility complex (MHC) class II (Ia) in primary cultures of newborn murine astrocytes and microglial cells. Astrocytes expressed very low levels of Ia molecules under basal culture conditions but these molecules could be induced with IFN-gamma. IL-1 beta in doses ranging from 1 to 100 units/ml inhibited the level of IFN-gamma induced Ia expression on astrocytes, and this inhibition was dose-dependent (mean maximum inhibition of 53 +/- 5% in number of positive cells and 53 +/- 2.6% in mean fluorescence intensity in four separate experiments). IL-1 beta treatment had no effect on MHC class I induction by IFN-gamma in the astrocytes. In contrast, microglial cells expressed Ia molecules under basal culture conditions, and this expression was enhanced by IFN-gamma treatment. Both basal and IFN-gamma induced Ia expression on microglia were resistant to IL-1 beta treatment in doses ranging from 1 to 100 units/ml. These results indicate that Ia expression is differentially regulated on astrocytes and microglial cells and that IL-1 beta may have an important immune regulatory function in the central nervous system.
authors.affiliation: Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, Bethesda, MD.
authors.forename: M E
authors.initials: ME
authors.lastname: Smith
authors.forename: D E
authors.initials: DE
authors.lastname: McFarlin
authors.forename: S
authors.initials: S
authors.lastname: Dhib-Jalbut
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000037:antagonists & inhibitors; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009457:Neuroglia / Q000166:cytology / Q000276:immunology*; D011994:Recombinant Proteins
chemical_list: D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D011994:Recombinant Proteins; D007371:Interferon-gamma