A protein inhibitor, extracted from Hela cells, which recognises a cell surface protease on preneoplastic cells obtained from cervical smears.
Hela cells originate from a clone of cervical carcinoma cells. The cytoplasm of Hela cells contains a protein which recognises and inhibits a proteolytic enzyme (guanidino-benzoatase) on the surface of Hela cells. This same protease is present on the surface of abnormal epithelial cells obtained from cervical smears, enabling a rhodamine labelled inhibitor extracted from Hela cells to locate abnormal cervical cells in monolayer spreads.
Steven
F S
FS
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
Johnson
J
J
Eason
P
P
D014408:Biomarkers, Tumor / Q000032:analysis; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D002584:Cervix Uteri / Q000201:enzymology* / Q000473:pathology; D010450:Endopeptidases / Q000032:analysis* / Q000378:metabolism; D004848:Epithelium / Q000201:enzymology / Q000473:pathology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D011230:Precancerous Conditions / Q000201:enzymology* / Q000473:pathology; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000473:pathology; D014626:Vaginal Smears
D014408:Biomarkers, Tumor; D011480:Protease Inhibitors; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases