Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway.
A 3.5-kb DNA fragment from the Clarke and Carbon Escherichia coli genomic clone, pLC37-44, was sequenced on both strands. Part of the zwf gene, encoding glucose-6-phosphate dehydrogenase, and all of the edd and eda genes, encoding 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase, respectively, of the Entner-Doudoroff pathway, were identified. These data are compared with those of Egan et al. [J. Bacteriol. 174 (1992) 4638-4646] and important differences were noted.
Carter
A T
AT
AFRC Institute of Food Research, Norwich Research Park, UK.
Pearson
B M
BM
Dickinson
J R
JR
Lancashire
W E
WE
D000446:Aldehyde-Lyases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D001426:Bacterial Proteins; D005954:Glucosephosphate Dehydrogenase; D000446:Aldehyde-Lyases; C100131:phospho-2-keto-3-deoxy-gluconate aldolase; D006836:Hydro-Lyases; C022362:phosphogluconate dehydratase