Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats.
Riboflavin deficiency in rats resulted in a reduction in the transfer of 59Fe from an intragastric dose to plasma compared to age-matched or weight-matched controls. The uptake of iron by brush-border membrane vesicles made from intestinal mucosa of riboflavin-deficient rats was much less than identically-prepared vesicles from control groups. Although the mucosal content of 59Fe was smaller in riboflavin-deficient rats thirty minutes after dosing, the relative distribution of 59Fe between the mucosal iron-binding proteins, ferritin and transferrin, was not changed compared to the control groups. These studies suggest that the impairment in iron absorption in riboflavin deficiency is primarily the result of a reduced uptake of iron into the mucosal cell and not a redistribution of iron between iron-binding proteins inside the mucosal cell.
Butler
B F
BF
Department of Chemistry, University of Richmond, VA 23173.
Topham
R W
RW
D000818:Animals; D001835:Body Weight; D005293:Ferritins / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis; D007413:Intestinal Mucosa / Q000378:metabolism*; D007501:Iron / Q000097:blood / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D009929:Organ Size; D051381:Rats; D012257:Riboflavin Deficiency / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism
D006454:Hemoglobins; D014168:Transferrin; D005293:Ferritins; D007501:Iron