Characterization of the radish mitochondrial orfB locus: possible relationship with male sterility in Ogura radish.
The orfB locus of the normal (fertile) and Ogura (male-sterile) radish mitochondrial genomes has been characterized in order to determine if this region, which has previously been correlated with cytoplasmic male sterility (CMS) in Brassica napus cybrids (Bonhomme et al. 1991; Temple et al. 1992), could also be involved in radish CMS. In normal radish, orfB is expressed as a 600-nucleotide (nt) transcript. In Ogura radish, orfB is present as the second gene of a 1200-nt transcript that also contains a 138-codon open reading frame (orf138). Sequences showing similarity to orf138 are present in normal radish, but are not expressed.
Krishnasamy
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Department of Chemistry, Miami University, Oxford, OH 45056.
Makaroff
C A
CA
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004272:DNA, Mitochondrial / Q000235:genetics*; D005298:Fertility / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010944:Plants / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial