The Conneulor: unbiased estimation of connectivity using physical disectors under projection.
The Euler number and the connectivity of an arbitrary object is defined, and it is illustrated why the connectivity of an n-dimensional object cannot be estimated in an (n-1)-dimensional section. The disector--principle for 3-D counting of the Euler--events is illustrated in cancellous bone. The correct handling for unbiased counting of events at artificial edges is outlined. A nomogram for predicting the precision of an estimate is provided.
Gundersen
H J
HJ
Stereological Research Laboratory, University of Aarhus, Denmark.
Boyce
R W
RW
Nyengaard
J R
JR
Odgaard
A
A
D001842:Bone and Bones / Q000033:anatomy & histology*; D006801:Humans; D015233:Models, Statistical