Non-injection methods for the production of embryonic stem cell-embryo chimaeras.
The simple aggregation of pluripotent ES cells with morulae-stage embryos can result in viable chimaeras at a similar frequency to that of blastocyst injection. The savings in time and equipment inherent in the aggregation techniques, however, make this approach well worth considering.
Wood
S A
SA
Department of Molecular Embryology, Max-Planck Institut für Immunbiologie, Freiburg, Germany.
Allen
N D
ND
Rossant
J
J
Auerbach
A
A
Nagy
A
A
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002449:Cell Aggregation; D002478:Cells, Cultured; D002678:Chimera; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D004626:Embryology / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D009028:Morula / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*