title: Crystallization of a chitosanase from Streptomyces N174.
abstract: Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 A resolution. The crystals belong to the monoclinic space group P2(1) with unit cell parameters a = 56.4 A, b = 59.6 A, c = 86.1 A and beta = 96.6 degrees. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas, Austin 78712.
authors.forename: E
authors.initials: E
authors.lastname: Marcotte
authors.forename: P J
authors.initials: PJ
authors.lastname: Hart
authors.forename: I
authors.initials: I
authors.lastname: Boucher
authors.forename: R
authors.initials: R
authors.lastname: Brzezinski
authors.forename: J D
authors.initials: JD
authors.lastname: Robertus
mesh_terms: D003460:Crystallization; D006026:Glycoside Hydrolases / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D006026:Glycoside Hydrolases; C019868:chitosanase