Structure of three acidic O-linked carbohydrate chains of porcine zona pellucida glycoproteins.
Structural analysis by 1D and 2D 1H NMR spectroscopy of three acidic O-linked oligosaccharide alditols, released from porcine zona pellucida glycoproteins by alkaline borohydride treatment, afforded the following structures: Gal beta 1-4(6SO4)GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-3GalNAc- ol Neu5Gc alpha 2-3Gal beta 1-4(6SO4-)GlcNAc beta 1-3Gal beta 1- 4GlcNAc beta 1-3Gal beta 1-3GalNAc-ol Neu5Ac alpha 2-3Gal beta 1-4(6SO4-)GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1- 3Gal beta 1-3GalNAc-ol These oligosaccharides are the smallest compounds that contain the structural elements which are present in the acidic, high-molecular mass O-linked carbohydrate chains of porcine zona pellucida glycoproteins.
Hokke
C H
CH
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Damm
J B
JB
Kamerling
J P
JP
Vliegenthart
J F
JF
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006023:Glycoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000737:chemistry; D013552:Swine; D015044:Zona Pellucida / Q000737:chemistry*
D006023:Glycoproteins; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid