Properties of alpha 1-antitrypsin secreted by human adenocarcinoma cell lines.
alpha 1-Antitrypsin; (alpha 1-AT) produced by various human carcinoma (non-hepatoma) cell lines were analyzed. Five out of eight cell lines secreted detectable amounts of alpha 1-AT into the conditioned media. All were adenocarcinoma cell lines. The tumor cell-derived alpha 1-ATs had higher molecular weights (MW) than the normal plasma form. Most of this difference was an overall reflection of altered N-glycosylation. As judged by binding of lectins, the glycosylation had shifted towards higher levels of triantennary oligosaccharides and higher levels of fucosylation. The conditioned media also contained lower MW alpha 1-AT species, possibly, proteolytically cleaved forms.
Kataoka
H
H
Second Department of Pathology, Miyazaki Medical College, Japan.
Seguchi
K
K
Inoue
T
T
Koono
M
M
D000230:Adenocarcinoma / Q000378:metabolism*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases; D006801:Humans; D037102:Lectins / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000515:alpha 1-Antitrypsin / Q000378:metabolism*
D037102:Lectins; D000515:alpha 1-Antitrypsin; D006026:Glycoside Hydrolases