Capsular types of Vibrio vulnificus: an analysis of strains from clinical and environmental sources.
Vibrio vulnificus produces a capsular polysaccharide (CPS) that is essential for virulence. CPS from V. vulnificus clinical strain MO6-24 has been purified and the structure determined. In preliminary screening with antisera raised to MO6-24 CPS, 4 (19%) of 21 clinical isolates (including MO6-24), but none of 67 environmental V. vulnificus isolates, agglutinated with anti-MO6-24 antisera (P = .003). CPS was isolated from a subset of 12 clinical and 7 environmental isolates and analyzed by high-performance anion-exchange chromatography and one-dimensional nuclear magnetic resonance. MO6-24 and 1 other serologically positive strain had identical CPS structures; the other 2 serologically positive strains had substitutions in two of four sugar residues. Thirteen other capsular types were identified among the remaining 15 strains from which CPS was extracted.
Hayat
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U
Department of Medicine, University of Maryland School of Medicine, Baltimore.
Reddy
G P
GP
Bush
C A
CA
Johnson
J A
JA
Wright
A C
AC
Morris
J G
JG
D000372:Agglutination Tests; D016667:Bacterial Capsules / Q000737:chemistry / Q000145:classification* / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D014733:Vibrio / Q000737:chemistry / Q000145:classification* / Q000276:immunology; D014735:Vibrio Infections / Q000382:microbiology; D014871:Water Microbiology
D011135:Polysaccharides, Bacterial