Sulfation of hypertensive and hypotensive drugs by monkey brain phenol sulfotransferase.
The substrate specificity and affinity of two forms of phenol sulfotransferase (PST) from Rhesus macaque brain cortex were studied. Catecholamines, their methylated metabolites (normetanephrine, metanephrine) and methylated precursor, alpha-methylDOPA, were examined as substrates for both the cationic (PST I) and the anionic (PST II) forms of the enzyme. Sulfation of hypertensive drugs (phenylephrine, octopamine, metaraminol), hypotensive drugs (alpha-methylDOPA, minoxidil), and related agents without a free hydroxy group on the benzene ring were also studied. Results indicated that both PST forms sulfated alpha-methylDOPA and minoxidil, but only PST II transferred the sulfate group to catecholamines and most of the adrenergic agents examined.
Barańczyk-Kuźma
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Department of Biochemistry, Institute of Biopharmacy, Warsaw Medical School, Poland.
Drobisz
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Audus
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Borchardt
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RT
D000818:Animals; D000959:Antihypertensive Agents / Q000378:metabolism*; D015239:Arylsulfotransferase / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D008253:Macaca mulatta; D008680:Metaraminol / Q000378:metabolism; D008750:Methyldopa / Q000378:metabolism; D008914:Minoxidil / Q000378:metabolism; D009655:Octopamine / Q000378:metabolism; D010656:Phenylephrine / Q000378:metabolism; D013379:Substrate Specificity; D013431:Sulfates / Q000378:metabolism*; D013566:Sympathomimetics / Q000378:metabolism*
D000959:Antihypertensive Agents; D013431:Sulfates; D013566:Sympathomimetics; D009655:Octopamine; D010656:Phenylephrine; D008750:Methyldopa; D008914:Minoxidil; D008680:Metaraminol; D015239:Arylsulfotransferase