title: Analysis of interferon-alpha 2 sequences in human genomic DNA.
abstract: We have analyzed the genomic DNA sequence corresponding to the human interferon-alpha 2 (IFN-alpha 2) gene locus. In human lymphoblastoid Namalwa cells, we have detected sequences corresponding to IFN-alpha 2b and -2c, while in human KG-1 cells both IFN-alpha 2a and -2b were present. However, in 100 independent IFN-alpha 2 clones derived from 20 unrelated Caucasian volunteers, we found only sequences corresponding to IFN-alpha 2b. Statistical analysis of this result suggests that the sequences corresponding to IFN-alpha 2a and -2c are either rare allelic variants of this gene, occurring in only a minority of the Caucasian population, or are restricted to transformed cell lines.
authors.affiliation: Department of Cell Biology, Wellcome Foundation Ltd., Beckenham, Kent, England.
authors.forename: D
authors.initials: D
authors.lastname: Gewert
authors.forename: C
authors.initials: C
authors.lastname: Salom
authors.forename: K
authors.initials: K
authors.lastname: Barber
authors.forename: S
authors.initials: S
authors.lastname: Macbride
authors.forename: H
authors.initials: H
authors.lastname: Cooper
authors.forename: A
authors.initials: A
authors.lastname: Lewis
authors.forename: J
authors.initials: J
authors.lastname: Wood
authors.forename: S
authors.initials: S
authors.lastname: Crowe
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D015894:Genome, Human; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D004247:DNA