title: Determination of the disulphide bridge arrangement of bovine histidine-rich glycoprotein.
abstract: Histidine-rich glycoprotein (HRG) was purified from bovine plasma and the disulphide bridge arrangement established. Disulphide-bridged peptides were obtained from peptic and tryptic degradation of native bovine HRG. Twelve half-cystine residues were found in bovine HRG (compared to sixteen cysteines in human HRG), all involved in the formation of six disulphide bridges connecting Cys-1 to Cys-12, Cys-2 to Cys-3, Cys-4 to Cys-5, Cys-6 to Cys-11, Cys-7 to Cys-8, and Cys-9 to Cys-10. Additional sequence analysis of 14C-carboxymethylated chymotryptic and Staphylococcus aureus V8 protease generated peptides and CNBr-fragments of bovine HRG yielded a partial amino acid sequence of bovine HRG constituting 78% of the sequence when compared to the human cDNA sequence.
authors.affiliation: Department of Molecular Biology, University of Aarhus, Denmark.
authors.forename: C B
authors.initials: CB
authors.lastname: Sørensen
authors.forename: H
authors.initials: H
authors.lastname: Krogh-Pedersen
authors.forename: T E
authors.initials: TE
authors.lastname: Petersen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine / Q000737:chemistry; D002417:Cattle; D003545:Cysteine / Q000032:analysis; D004220:Disulfides / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011506:Proteins / Q000032:analysis*; D017386:Sequence Homology, Amino Acid; D000099053:Histidine-Rich Glycoprotein
chemical_list: D004220:Disulfides; D006023:Glycoproteins; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein; D001216:Asparagine; D003545:Cysteine