Mechanisms involved in the loss of antibody-mediated adherence of macrophages to lung-stage schistosomula of Schistosoma mansoni in vitro.
Sera from rabbits vaccinated with irradiated cercariae mediated cell (P388D1 or mouse peritoneal macrophage) adherence to lung-stage schistosomula (LS) but such antibody-mediated cell adherence was short-lived in contrast to cell adherence to mechanically transformed schistosomula (MS). Thus LS lost 50% of their adherent cells within 3-6 h in culture and up to 90% by 24 h, whereas adherence to MS was undiminished during this time. Rapid loss of adherent cells was unique to schistosomula that had developed to the lung stage because schistosomula recovered from the skin up to 3 days post-infection did not exhibit the rapid cell loss shown by 3-day LS. To determine whether cell loss was caused by loss of surface antigenicity during culture LS were cultured on their own for up to 24 h and at various intervals samples of schistosomula were tested for antigenicity by addition of immune serum and cells. Levels of adherence to both MS and LS were maintained throughout the incubation period. When antibody-opsonized schistosomula were washed and indicator cells added at progressive intervals, persistence of adherence was again demonstrated, showing that antibody binding to LS had not promoted surface antigen loss or degradation of bound antibody. It was then shown, by adding fresh macrophages to cultures up to 24 h old that LS which had lost their adherent cells nevertheless retained bound antibody, and comparison of adherence of 'used' and 'fresh' cells to MS and LS showed that the cytoadherence properties of macrophages were not significantly reduced during their culture with LS from which cells had been lost.(ABSTRACT TRUNCATED AT 250 WORDS)
Lawson
B W
BW
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine.
Bickle
Q D
QD
Taylor
M G
MG
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007700:Kinetics; D007814:Larva / Q000187:drug effects / Q000276:immunology; D007941:Leukemia P388; D008168:Lung / Q000469:parasitology; D008264:Macrophages / Q000187:drug effects / Q000469:parasitology*; D008297:Male; D051379:Mice; D009895:Opsonin Proteins / Q000276:immunology; D011691:Puromycin / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012550:Schistosoma mansoni / Q000187:drug effects / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D007106:Immune Sera; D009895:Opsonin Proteins; D003609:Dactinomycin; D011691:Puromycin