Immunogenicity in rabbits and monkeys of influenza ISCOMs conjugated with repeated sequences of the Plasmodium falciparum antigen Pf155/RESA.
Rabbits and monkeys were immunized with two fusion proteins, ZZ-M3 and ZZ-M5, coupled to pre-formed influenza virus membrane glycoprotein ISCOMs. The fusion proteins comprise two IgG-binding domains from staphylococcal protein A (ZZ) and repeated amino acid sequences from the C-terminal (M3) or central (M5) repeat regions of the Plasmodium falciparum antigen Pf155/RESA. The induced antibody responses were of long duration, could be efficiently boosted and were comparable to those obtained with Freund's Adjuvant. The produced antibodies reacted with M3, M5, protein A and the influenza glycoprotein, recognized Pf155/RESA and inhibited merozoite invasion in vitro. These results suggest that coupling of immunogens to pre-formed ISCOMs may be a basis for construction of multivalent subunit vaccines.
Sjölander
A
A
Department of Immunology, Stockholm University, Sweden.
Hansson
M
M
Lövgren
K
K
Wåhlin
B
B
Berzins
K
K
Perlmann
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004306:Dose-Response Relationship, Immunologic; D016882:ISCOMs / Q000737:chemistry / Q000276:immunology*; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D016778:Malaria, Falciparum / Q000276:immunology / Q000517:prevention & control; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology* / Q000627:therapeutic use; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000276:immunology; D013291:Streptococcus / Q000276:immunology; D013997:Time Factors; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D001426:Bacterial Proteins; D016882:ISCOMs; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D010446:Peptide Fragments; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D013205:Staphylococcal Protein A; D014614:Vaccines, Synthetic; C050256:ring-infected erythrocyte surface antigen (RESA), Plasmodium falciparum