Chromosome rearrangements recovered following transformation of Neurospora crassa.
New chromosome rearrangements were found in 10% or more of mitotically stable transformants. This was shown for transformations involving a variety of different markers, vectors and recipient strains. Breakpoints were randomly distributed among the seven linkage groups. Controls using untransformed protoplasts of the same strains contained almost no rearrangements. A study of molecularly characterized Am+ transformants showed that rearrangements are frequent when multiple ectopic integration events have occurred. In contrast, rearrangements are absent or infrequent when only the resident locus is restored to am+ by a homologous event. Sequences of the transforming vector were genetically linked to breakpoints in 6 of 10 translocations that were examined using Southern hybridization or colony blots.
Perkins
D D
DD
Department of Biological Sciences, Stanford University, California 94305-5020.
Kinsey
J A
JA
Asch
D K
DK
Frederick
G D
GD
D015825:Chromosomes, Fungal; D004271:DNA, Fungal; D008040:Genetic Linkage; D009492:Neurospora crassa / Q000235:genetics*; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D004271:DNA, Fungal