A complex ensemble of cis-regulatory elements controls the expression of a Vicia faba non-storage seed protein gene.
We have identified cis-regulatory elements within the 5'-upstream region of a Vicia faba non-storage seed protein gene, called usp, by studying the expression of usp-promoter deletion fragments fused to reporter genes in transgenic tobacco seeds. 0.4 kb of usp upstream sequence contain at least six, but probably more, distinct cis-regulatory elements which are responsible for seemingly all quantitative, spatial and temporal aspects of expression. Expression-increasing and -decreasing elements are interspersed and include an AT-rich sequence, a G-box element and a CATGCATG motif. The latter acts as a negative element in contrast to what has been found for the same motif in legumin- and vicilin-type seed storage protein gene promoters. Seed specificity of expression is mainly determined by the -68/+51 region which confers, however, only very low levels of expression. The data support the combinatorial model of promoter function.
Fiedler
U
U
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Filistein
R
R
Wobus
U
U
Bäumlein
H
H
D001483:Base Sequence; D004252:DNA Mutational Analysis; D015536:Down-Regulation; D004742:Enhancer Elements, Genetic / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D005786:Gene Expression Regulation; D017343:Genes, Plant / Q000235:genetics; D005966:Glucuronidase / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012639:Seeds / Q000235:genetics*; D017384:Sequence Deletion; D013997:Time Factors; D014018:Tissue Distribution; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C082498:usp protein, Vicia faba; D005966:Glucuronidase