title: Production of cytolethal distending toxin (CLDT) by Campylobacter fetus subsp. fetus isolated from calves.
abstract: Cytolethal distending toxin (CLDT) production by Campylobacter fetus subsp. fetus isolated from calves was examined using CHO cells. Twenty-five of the 26 strains tested were positive for CLDT with titer of ranging 1:8 to 1:2,048. CLDT positive strains were divided into low and high cytotoxin titer groups. Isolates from diarrhea cases tended to produce a significant amount of CLDT compared with isolates from liver. CLDT produced by C. fetus showed no effect on Y-1 cells and was heat-labile and trypsin-sensitive.
authors.affiliation: Kyushu Branch Laboratory, National Institute of Animal Health, Kagoshima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohya
authors.forename: K
authors.initials: K
authors.lastname: Tominaga
authors.forename: M
authors.initials: M
authors.lastname: Nakazawa
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000096:biosynthesis* / Q000633:toxicity; D016466:CHO Cells; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D002168:Campylobacter fetus / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004355:Drug Stability; D005243:Feces / Q000382:microbiology; D005766:Gastrointestinal Contents / Q000382:microbiology; D006358:Hot Temperature; D014357:Trypsin
chemical_list: D001427:Bacterial Toxins; C067044:cytolethal distending toxin; D014357:Trypsin