The properties of an HBV surface antigen protein carrying the binding site for the receptor of hepatocytes--its formation of surface antigen particles and secretion from discrete cell lines.
A human hepatitis B virus (HBV) gene, which encodes the major surface antigen protein (S protein) carrying the hepatocyte receptor-binding site, was constructed with site-directed mutagenesis and in vitro recombination. When expressed in monkey kidney cell line COS-M6, this gene product (S309 protein) formed surface antigen (HBsAg) particles and secreted from the cells. It was stable within the cells and in the culture medium and could be immunoprecipitated with antisera directed against plasma-derived HBsAg or synthetic preS1 polypeptide. Isopycnic CsCl gradient centrifugation showed that the density of S309 protein particles (1.25 g/ml) was slightly higher than that of S protein particles. The S309 protein was readily secretable from hepatoma cell lines, and the amount secreted was comparable to that of the S protein. By contrast, only about 10% of the S309 protein was secreted from COS-M6 cells, and its appearance in culture medium was delayed. The efficiency of the secretion of the S309 protein can be improved when it is coexpressed with the S protein.
Yu
X M
XM
Shanghai Institute of Biochemistry, Academia Sinica, PRC.
Li
Z P
ZP
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D015870:Gene Expression; D005814:Genes, Viral; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000737:chemistry; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000166:cytology; D008114:Liver Neoplasms, Experimental / Q000473:pathology; D010957:Plasmids; D011946:Receptors, Antigen; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D006514:Hepatitis B Surface Antigens; D011946:Receptors, Antigen; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; C030500:hepatitis B surface antigen receptor