Ouabain causes kaliuresis and works synergistically with aldosterone in vivo.
The effect of ouabain on urinary Na and K excretion was compared with that of aldosterone by injecting 0.1 or 0.5 mg ouabain or 0.25 micrograms aldosterone. The administration of 0.25 micrograms aldosterone caused sodium-retention without kaliuresis, whereas the administration of 0.5 mg ouabain caused kaliuresis without sodium-retention. These results indicate that ouabain is a kaliuretic agent. The effect of a combination of aldosterone and ouabain was evaluated by injecting 0.25 micrograms aldosterone simultaneously with 0.1 or 0.5 mg ouabain. The combination of aldosterone and ouabain caused both sodium-retention and kaliuresis. These results indicate that ouabain works synergistically with aldosterone to increase mineralocorticoid action by inducing K depletion from the cells in various tissues.
Sekihara
H
H
Third Department of Internal Medicine, University of Tokyo Faculty of Medicine, Japan.
Yazaki
Y
Y
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D004357:Drug Synergism; D008297:Male; D008901:Mineralocorticoids / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000652:urine
D008901:Mineralocorticoids; D000450:Aldosterone; D010042:Ouabain; D012964:Sodium; D011188:Potassium