title: Bacterial adhesion to protein-coated hydrogels.
abstract: Extended wear soft contact lenses have been implicated in the increased occurrence of corneal bacterial infections. This research investigated the effects of polymer chemistry, water content, and pre-sorbed proteins upon the adherence of Pseudomonas aeruginosa to model hydrogels with chemistries similar to those of extended wear soft contact lenses. The hydrogels were exposed to washed suspensions of P. aeruginosa in a laminar flow cell. Albumin, fibrinogen, desialylated fibrinogen, or mucin were deposited on the hydrogels before exposure to the bacteria. Results showed that with or without protein pre-exposure, bacterial adhesion decreased as water content increased. In the presence of the sorbed protein, the number of adherent bacteria increased by about 45%, and all four proteins caused similar increases in adhesion. Bacterial adhesion was not significantly influenced by the presence of sialic acid residues in the pre-sorbed protein.
authors.affiliation: Chemical Engineering Department, Brigham Young University, Provo, UT 84602.
authors.forename: A D
authors.initials: AD
authors.lastname: Cook
authors.forename: R D
authors.initials: RD
authors.lastname: Sagers
authors.forename: W G
authors.initials: WG
authors.lastname: Pitt
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002417:Cattle; D003263:Contact Lenses, Hydrophilic; D005340:Fibrinogen / Q000494:pharmacology; D005782:Gels; D006801:Humans; D009077:Mucins / Q000494:pharmacology; D019158:N-Acetylneuraminic Acid; D011108:Polymers / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000472:pathogenicity; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012794:Sialic Acids / Q000494:pharmacology; D013552:Swine
chemical_list: D005782:Gels; D009077:Mucins; D011108:Polymers; D011506:Proteins; D012794:Sialic Acids; D012710:Serum Albumin, Bovine; D005340:Fibrinogen; D019158:N-Acetylneuraminic Acid