Nutrient-responsive promoter elements of the V4 gene of Dictyostelium discoideum.
Expression of the V4 gene of Dictyostelium discoideum is required for the transition from growth to development in response to an altered nutrient environment. In addition, the expression itself is sensitive to the types and amounts of nutrients supporting growth. We describe the structure of the two copies of the V4 gene and the relationship between these genes and the two V4 mRNA species produced during growth. In addition, three regions were identified within the upstream sequences of the V4b gene that are important for proper transcription. At least two of the regions can, independently of the others, confer deactivation of transcription upon initiation of development and thus serve as redundant regulatory sequences. However, the regions are differentially responsive to the types and amounts of nutrients present in the cell's environment and thus are distinct from one another.
McPherson
C E
CE
Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235.
Singleton
C K
CK
D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D003470:Culture Media / Q000494:pharmacology; D004023:Dictyostelium / Q000235:genetics*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D017125:Genes, Protozoan / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015800:Protozoan Proteins; D012322:RNA Precursors / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D003470:Culture Media; D005656:Fungal Proteins; D015800:Protozoan Proteins; D012322:RNA Precursors; D011993:Recombinant Fusion Proteins; C082562:V4 protein, Dictyostelium; D015500:Chloramphenicol O-Acetyltransferase