A mitochondrial heat shock protein from Crithidia fasciculata.
MCP72 is a mitochondrial hsp70 protein from the trypanosomatid Crithidia fasciculata. An MCP72 cDNA clone was isolated from a C. fasciculata cDNA library by screening with antiserum specific for the homologous protein of Trypanosoma cruzi [9]. The MCP72 cDNA encodes a polypeptide of 663 amino acids which is 84% identical to the Trypanosoma cruzi protein and 56% identical to the Escherichia coli hsp70 protein DnaK. MCP72 is less similar to other hsp70 proteins. Native MCP72 was purified to homogeneity by ATP-agarose affinity chromatography. Comparison of its N-terminal amino acid sequence with that deduced from the cDNA sequence shows that 20 amino acid residues had been cleaved from the N-terminus; this sequence probably represents a mitochondrial import signal which is cleaved during translocation into the mitochondrion. Fluorescence microscopy, using antibodies specific for MCP72, indicates that the protein is concentrated in a region of the mitochondrial matrix which surrounds the kinetoplast.
Effron
P N
PN
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205.
Torri
A F
AF
Engman
D M
DM
Donelson
J E
JE
Englund
P T
PT
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016831:Crithidia fasciculata / Q000235:genetics / Q000378:metabolism*; D016054:DNA, Protozoan / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014349:Trypanosoma cruzi / Q000235:genetics / Q000378:metabolism
D016054:DNA, Protozoan; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases