title: Prolactin induces maturation of glucose sensing mechanisms in cultured neonatal rat islets.
abstract: The effects of PRL treatment on insulin content and secretion, and 86Rb and 45Ca fluxes from neonatal rat islets maintained in culture for 7-9 days were studied. PRL treatment enhanced islet insulin content by 40% and enhanced early insulin secretion evoked by 16.7 mM glucose. Insulin release stimulated by oxotremorine-M, a muscarinic agonist, in the presence of glucose (8.3 or 16.7 mM) was unchanged by PRL treatment. However, PRL treatment potentiated phorbol 12,13-dibutyrate-stimulated insulin secretion in the presence of the above glucose concentrations. PRL treatment potentiated the reduction in 86Rb efflux induced by glucose or tolbutamide and enhanced the increase in 86Rb efflux evoked by diazoxide. PRL treatment slightly potentiated the increment in 45Ca uptake induced by high concentrations of K+, but failed to affect the increment evoked by 16.7 mM glucose. Since glucose-induced 45Ca uptake was not affected by PRL, we suggest that the enhancement in first phase insulin secretion evoked by glucose in the PRL-treated islets occurs at a step in the secretory process that may involve protein kinase-C. These data further support observations that PRL treatment increases islet sensitivity to glucose.
authors.affiliation: Laboratory of Cell Biology and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: A C
authors.initials: AC
authors.lastname: Boschero
authors.forename: S C
authors.initials: SC
authors.lastname: Crepaldi
authors.forename: E M
authors.initials: EM
authors.lastname: Carneiro
authors.forename: E
authors.initials: E
authors.lastname: Delattre
authors.forename: I
authors.initials: I
authors.lastname: Atwater
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002132:Calcium Radioisotopes / Q000378:metabolism; D002478:Cells, Cultured; D004357:Drug Synergism; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D010095:Oxotremorine / Q000031:analogs & derivatives / Q000494:pharmacology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats; D012414:Rubidium Radioisotopes / Q000378:metabolism; D014044:Tolbutamide / Q000494:pharmacology
chemical_list: D002132:Calcium Radioisotopes; D007328:Insulin; D012414:Rubidium Radioisotopes; D015240:Phorbol 12,13-Dibutyrate; D010095:Oxotremorine; C042743:oxotremorine M; D011388:Prolactin; D014044:Tolbutamide; D005947:Glucose; D011188:Potassium