Enteroendocrine peptides in a canine model of orthotopic jejunoileal autotransplantation.
The enteroendocrine cells of the small bowel provide a rich source of regulatory peptides involved in the modulation of gastrointestinal function. Recent work from our laboratory showed that in situ neural isolation (autotransplantation) of the jejunoileum produced marked changes in tissue expression of several neuropeptides. In the present study, we examined the influence of extrinsic innervation on the tissue expression of endocrine peptides localized to various regions of the gastrointestinal tract. Concentrations of immunoreactive gastric inhibitory polypeptide (GIP), neurotensin (NT) and peptide tyrosine tyrosine (PYY) in fasting plasma and regional tissue biopsies were determined before and at varying time points (2, 6, 12 weeks) after a model of canine orthotopic jejunoileal autotransplantation. GIP was not altered in plasma or tissue at any time point. Plasma concentrations of NT and PYY increased after autotransplantation. Following a decrease in tissue concentrations two weeks after autotransplantation, NT increased progressively from 2 to 6 to 12 weeks, reaching a maximal increase of 895% over baseline in proximal ileum. Tissue concentrations of PYY followed much the same pattern as NT, but these trends never achieved statistical significance. Chromatographic characterization of tissue biopsy extracts revealed molecular heterogeneity of NT-like immunoreactivity, while GIP and PYY immunoreactivity coeluted as single species with authentic standards. Taken together with our earlier observations, it appears that disruption of extrinsic and intrinsic neural continuity to the jejunoileum (autotransplantation) does not affect gut endocrine peptides such as GIP and PYY to the same extent as enteric neuropeptides. NT has been localized to neural as well as endocrine cells and is involved in the temporal adaptive response to autotransplantation.
Nelson
D K
DK
Gastroenterology Research Unit, Mayo Clinic, Rochester, MN.
Furlow
B L
BL
Go
V L
VL
Sarr
M G
MG
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000378:metabolism; D004285:Dogs; D004386:Duodenum / Q000378:metabolism; D005260:Female; D005749:Gastric Inhibitory Polypeptide / Q000097:blood / Q000378:metabolism; D007082:Ileum / Q000378:metabolism / Q000637:transplantation*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism / Q000637:transplantation*; D009479:Neuropeptides / Q000097:blood / Q000378:metabolism*; D009496:Neurotensin / Q000097:blood / Q000378:metabolism; D019894:Peptide YY; D010455:Peptides / Q000097:blood / Q000378:metabolism; D011863:Radioimmunoassay; D014182:Transplantation, Autologous
D009479:Neuropeptides; D010455:Peptides; D019894:Peptide YY; D009496:Neurotensin; D005749:Gastric Inhibitory Polypeptide