Regulation of the annexin Icp35 gene in transfected mammary gland cell lines.
We have tested the ability of the 5' flanking region of the lactogen-regulated annexin Icp35 gene to drive the expression of the bacterial chloramphenicol acetyltransferase (CAT) gene in T-47D, HBL-100 and HC11 cells. A construct containing 1,400 bp of the 5' flanking region including the putative promoter, and the first two exons and intervening sequence showed lower activity compared with a similar construct containing only 250 bp of the 5' flanking region. This indicated the presence of negative regulatory elements in the distal 5' flanking region. Experiments were done to determine the effect of lactogens (either ovine prolactin, or human growth hormone) on the cp35 promoter using various hormone treatments, growth conditions and serum supplements. We did not observe any effect of hormones on the expression of the cp35/CAT constructs under the conditions tested.
Uhrin
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College of Medicine, Dept. of Physiology and Biophysics, Cincinnati, Ohio.
Horseman
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D000818:Animals; D017305:Annexin A1 / Q000096:biosynthesis*; D015152:Blotting, Northern; D001940:Breast; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D010856:Columbidae; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology; D006801:Humans; D007438:Introns; D011388:Prolactin / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D016385:TATA Box; D014162:Transfection
D017305:Annexin A1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011388:Prolactin; D013006:Growth Hormone; D015500:Chloramphenicol O-Acetyltransferase