Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease.
The LYP1 gene of Saccharomyces cerevisiae was cloned by complementation in lysine-permease-deficient recipient yeast cells, and its nucleotide sequence was determined. An open reading frame of 1833 nucleotides was found encoding a polypeptide of 611 amino acids, with a calculated molecular weight of 68 118. Analysis of the deduced primary structure of the protein revealed ten membrane-spanning regions and three potential N-glycosylation sites. Analysis of the deduced sequence of protein LYP1 indicates homology with other yeast amino-acid permeases, in particular with CAN1, and also the lysine-specific permease of Escherichia coli. The strain transformed by a multi-copy plasmid harbouring the LYP1 gene, showed a 20-fold increase in the maximum velocity of lysine uptake over that in the wild type, with no changes in the affinity of the permease for its substrate.
Sychrova
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Institut de Biologie Moléculaire et Cellulaire, C.N.R.S., Strasbourg, France.
Chevallier
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MR
D000595:Amino Acid Sequence; D026923:Amino Acid Transport Systems, Basic; D001483:Base Sequence; D001692:Biological Transport / Q000502:physiology; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics* / Q000502:physiology; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D026923:Amino Acid Transport Systems, Basic; D004271:DNA, Fungal; C430023:LYP1 protein, S cerevisiae; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins