Direct double antibody sandwich immunoassay for Pseudomonas aeruginosa elastase.
A direct sandwich enzyme immunoassay was developed in order to quantify Pseudomonas aeruginosa elastase. As a solid phase the wells of a microtitre plate were coated with specific IgG and horseradish peroxidase labelled IgG was used as the second antibody. The detection limit of the assay was 0.26 ng/ml and a good agreement was found with elastolytic activity determined using elastin-Congo red. This assay was simple, specific, sensitive and reproducible, and permits the determination of low levels of elastase.
Jaffar-Bandjee
M C
MC
CERM, Hôpital Renée Sabran, Giens, Hyères, France.
Carrere
J
J
Lazdunski
A
A
Guy-Crotte
O
O
Galabert
C
C
D003550:Cystic Fibrosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010196:Pancreatic Elastase / Q000032:analysis* / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000276:immunology
D010196:Pancreatic Elastase