Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast.
The recognition that cellular regulatory circuitry is composed of antagonistic elements has made possible a new approach to the selection of regulatory genes, called the gene-gene interference method. The method was used to identify and isolate genes possibly related in an antagonistic way to protein kinase A. Two such genes were recovered: ART1 encodes a potential regulator of cytokinesis and KAI1 appears to be involved in the Start control. The principles of the gene-gene interference method are discussed, as well as its possible general use for 'walking' within the cellular regulatory networks of eukaryotes.
Daniel
J
J
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
D000595:Amino Acid Sequence; D015703:Antigens, CD; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002455:Cell Division / Q000235:genetics; D016386:Chromosome Walking / Q000379:methods*; D003001:Cloning, Molecular; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005809:Genes, Regulator; D005822:Genetic Vectors; D047908:Intracellular Signaling Peptides and Proteins; D051861:Kangai-1 Protein; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010957:Plasmids; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014170:Transformation, Genetic
D015703:Antigens, CD; D002135:Calcium-Binding Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D051861:Kangai-1 Protein; C091625:MID2 protein, S cerevisiae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins; C062214:SWI4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D011494:Protein Kinases