title: Bile secretion and liver regeneration in partially hepatectomized rats.
abstract: OBJECTIVE
  The purpose of this research was to study the correlation between bile secretion and the liver regeneration in the partially hepatectomized rat.
  
  
  BACKGROUND
  Significant alteration in bile formation and secretion is expected in the liver after hepatectomy. There is scant literature, however, about the effects of liver regeneration in bile secretion.
  
  
  METHODS
  The work was done in rats with 50% hepatectomy, 75% hepatectomy, and sham operation as the control. A chronic common bile duct fistula and a duodenal cannula were established for bile collection and the sample analysis on days 1, 3, 5, 7, and 9.
  
  
  RESULTS
  With size reduced in the liver after 50% and 75% hepatectomy, the total bile volume decreased 45.9% and 51.5%, bile salt independent flow decreased 59.3% and 64.9%, bile salt secretion rate decreased 36.1% and 43.4%, bile salt basal synthesis rate decreased 52.3% and 56.4%, phospholipid secretion rate decreased 52.6% and 68.0%, and cholesterol secretion rate decreased 54.3% and 72.4% from control on day 1, respectively. All changes returned to the control level in 3 to 9 days with accompanying increasing liver size during regeneration.
  
  
  CONCLUSIONS
  Alterations of total bile flow, bile salt independent flow, bile salt secretion rate, bile salt basal synthesis rate, and biliary lipid secretion after partial hepatectomy correlate with the liver regeneration rate in rats. Partial hepatectomy reduces the bile salt independent fraction calculated as per 100 g body weight rather than the dependent fraction. The study of bile salt and biliary lipid secretion is a useful method for monitoring synthetic function in liver regeneration in vivo.
authors.affiliation: Department of Surgery, University of Virginia Health Sciences Center, Charlottesville 22908.
authors.forename: H S
authors.initials: HS
authors.lastname: Xu
authors.forename: L K
authors.initials: LK
authors.lastname: Rosenlof
authors.forename: R S
authors.initials: RS
authors.lastname: Jones
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000032:analysis / Q000096:biosynthesis; D002784:Cholesterol / Q000378:metabolism; D006498:Hepatectomy; D008099:Liver / Q000502:physiology / Q000601:surgery; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001647:Bile Acids and Salts; D010743:Phospholipids; D002784:Cholesterol