Sequence polymorphisms of cDNA clones encoding the mite allergen Der p I.
Sequence information has been obtained from 5 cDNA clones encoding the major house dust mite allergen Der p I, including one which codes for the full length preproenzyme form of the molecule. All translated sequences were unique with 1-3 differences between each clone. 4/5 of the substitutions found were to residues found in Der f I and two positions had a substitution in more than one clone. The polymorphisms included residues already described to be in T cell epitopes, so as well as being necessary to consider them in the construction of synthetic allergens, the substitutions will be important for the interpretation of experimental and genetic studies.
Chua
K Y
KY
Western Australian Research Institute for Child Health, Perth.
Kehal
P K
PK
Thomas
W R
WR
D000485:Allergens / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D001483:Base Sequence; D002999:Clone Cells / Q000737:chemistry; D004247:DNA / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D000485:Allergens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D004247:DNA