title: Cellular and chemical mediators of type 1 hypersensitivity in calves infected with Ostertagia ostertagi: histamine, prostaglandin D2, prostaglandin E2 and leukotriene C4.
abstract: Plasma histamine, prostaglandin E2 (PG) D2, PGE2, and leukotriene (LT) C4 levels were determined in 26 Holstein steers before and after natural or experimental infection with Ostertagia ostertagi. Post-infection abomasal lymph was also assayed for PGD2, PGE2, and LTC4. Histamine determinations were performed on abomasal tissue from three locations. Results showed that: (1) tissue histamine levels increased in steers with type 2 ostertagiosis, (2) lymphatic PGD2 and PGE2 levels were increased, probably as a result of macrophage activity, (3) lymphatic LTC4 levels increased in steers with type 1 ostertagiosis, and were correlated with tissue eosinophilia, and (4) plasma levels of PGD2, PGE2, LTC4 and histamine were not useful for predicting worm burdens. These findings suggest a functional role for eosinophils and mast cells in the pathophysiology of ostertagiosis, through mediation of a type 1 hypersensitivity reaction.
authors.affiliation: Department of Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: D G
authors.initials: DG
authors.lastname: Baker
authors.forename: L J
authors.initials: LJ
authors.lastname: Gershwin
authors.forename: S N
authors.initials: SN
authors.lastname: Giri
authors.forename: C
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D015232:Dinoprostone / Q000096:biosynthesis; D006632:Histamine / Q000096:biosynthesis*; D007111:Immunity, Cellular; D008297:Male; D010029:Ostertagiasis / Q000276:immunology / Q000662:veterinary*; D015230:Prostaglandin D2 / Q000096:biosynthesis; D011453:Prostaglandins / Q000096:biosynthesis*; D013189:SRS-A / Q000096:biosynthesis*
chemical_list: D011453:Prostaglandins; D013189:SRS-A; D006632:Histamine; D015232:Dinoprostone; D015230:Prostaglandin D2