Variability among members of the Hor-2 multigene family.
The hordeins comprise the major prolamin storage proteins of barley. Two major and one minor gene families encode these alcohol-soluble proteins. The Hor-2 gene family encoding the B-hordeins has been estimated to contain 15-30 copies. Although several genes encoding B-hordeins have been cloned and sequenced, little is known about the mechanisms responsible for the generation of the enormous genetic variability at this locus. Polymerase chain reaction sequence amplification provided a simple technique that permitted the amplification of the Hor-2 gene family members from the genomes of several barley genotypes. Sequence analysis of clones permitted the identification of a region within the Hor-2 structural gene that appears to undergo recombinational and slippage-like gene conversion events. In this report we describe variability of the B-hordein genes, possible mechanisms responsible for it, and implications this may have on the evolution of prolamin-encoding gene families.
Kanazin
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Department of Plant and Soil Sciences, Montana State University, Bozeman 59717.
Ananiev
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Blake
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T
D001483:Base Sequence; D004247:DNA / Q000235:genetics; D017343:Genes, Plant; D014644:Genetic Variation; D005983:Glutens; D001467:Hordeum / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D010940:Plant Proteins; D005983:Glutens; D004247:DNA