Expression of a recombinant Entamoeba histolytica antigen in a Salmonella typhimurium vaccine strain.
The expression of a major surface antigen of the intestinal protozoal parasite Entamoeba histolytica in an attenuated Salmonella typhimurium vaccine strain is described. A polymerase chain reaction fragment derived from cDNA encoding the serine-rich Entamoeba histolytica protein, SREHP, was introduced into S. typhimurium chi 3987 (delta cya delta crp delta asd) using a plasmid expression vector (pYA292) containing the aspartate semialdehyde (asd) gene. S. typhimurium expressing recombinant SREHP as a SREHP/maltose binding protein fusion protein was administered orally to mice and gerbils (an important animal model for E. histolytica infection) and was recovered from splenic tissue in both species. Our study demonstrates the feasibility of expressing recombinant amoebic proteins in attenuated S. typhimurium strains, and shows that vaccine strains of S. typhimurium can successfully infect the gerbil, a widely used model for amoebic liver abscess and intestinal amoebiasis.
Cieslak
P R
PR
Department of Medicine, Washington University School of Medicine, St Louis, MO 63110.
Zhang
T
T
Stanley
S L
SL
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis*; D002352:Carrier Proteins / Q000235:genetics; D004748:Entamoeba histolytica / Q000235:genetics / Q000276:immunology*; D005260:Female; D005849:Gerbillinae; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000235:genetics; D016052:Protozoan Vaccines; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D013154:Spleen / Q000382:microbiology; D014614:Vaccines, Synthetic
D000953:Antigens, Protozoan; D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C073020:serine-rich protein, Entamoeba histolytica