[The effect of calcium ions on the activation of Escherichia coli hemolysin].
The calcium requirement for hemolytic activity Escherichia coli hemolysin is controversial. Thus, we aimed to study the calcium-dependent and independent hemolytic activity in an in vitro system. It was found that the free calcium in the cultivation medium might activate the hemolysin and once activated the hemolytic activity could not be inhibited by calcium chelator, EGTA. However, when the free calcium in the medium was chelated by EGTA before the inoculation of bacteria, the hemolytic activity was found to be almost entirely dependent upon the exogenous calcium supply to the test system. We also obtained some evidence showing that the calcium ions could take part in stabilization of this protein.
Abacioğlu
H
H
Dokuz Eylül Universitesi, Tip Fakültesi Mikrobiyoloji Anabilim Dali.
Yuluğ
N
N
D002118:Calcium / Q000494:pharmacology*; D003470:Culture Media; D004533:Egtazic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects
D003470:Culture Media; D006460:Hemolysin Proteins; D004533:Egtazic Acid; D002118:Calcium
Kalsiyum iyonlarinin Escherichia coli hemolizin aktivasyonundaki yeri.