Metabolic changes in the rat brain after acute and chronic ethanol intoxication: a 31P NMR spectroscopy study.
In this work, 31P phosphorus NMR (31P NMR) studies of the brain have been conducted in rats acutely and chronically intoxicated with ethanol. In both groups, changes in levels of high-energy phosphates were observed: increase of phosphocreatinine (PCr)/beta AaTP and PCr/inorganic phosphate (Pi) in acute and long-term ethanol exposure, and decrease of Pi/beta ATP after acute ethanol administration. These changes in high-energy phosphates, indicative of a reduction of adenosine triphosphate (ATP) and PCr consumption (PCr+ ADP+ H+ ATP+ Cr; ATP ADP+ Pi), suggest a reduction of cerebral metabolism both in acute and chronic ethanol exposure. In addition, in the group of rats chronically intoxicated with ethanol, there were variations in phosphodiester peak intensities (decrease of phosphomonoester (PME)/phosphodiester (PDE), increase of PDE/beta ATP), suggesting increased breakdown of membrane phospholipids. These changes could provide a metabolic explanation for the development of cerebral atrophy in chronic alcoholism.
Denays
R
R
Department of Neurology, St-Pierre Hospital, Brussels, Belgium.
Chao
S L
SL
Mathur-Devré
R
R
Jeghers
O
O
Frühling
J
J
Noël
P
P
Ham
H R
HR
D000255:Adenosine Triphosphate / Q000378:metabolism; D000435:Alcoholic Intoxication / Q000378:metabolism*; D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D000431:Ethanol / Q000009:adverse effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D010710:Phosphates; D010743:Phospholipids; D010725:Phosphocreatine; D000431:Ethanol; D000255:Adenosine Triphosphate