title: Characterization of a new class of tetracycline-resistance gene tet(S) in Listeria monocytogenes BM4210.
abstract: The nucleotide sequence of the tetracycline (Tc)-minocycline (Mc)-resistance determinant of plasmid pIP811 from Listeria monocytogenes BM4210 has been determined. The gene, designated tet(S), was identified by analysis of the start and stop codons as a coding sequence of 1923 bp, corresponding to a protein with a calculated M(r) of 72,912. The apparent 68-kDa size estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the protein characterized in a cell-free coupled transcription-translation system was in good agreement with the calculated value. The tet(S) gene product exhibits 79 and 72% amino acid identity with Tet(M) from Streptococcus pneumoniae and Tet(O) from Campylobacter coli, respectively. The distribution of tet(S) in strains of Gram+ and Gram- genera resistant to Tc (TcR) and Mc (McR) was studied by hybridization under high stringency using a 590-bp intragenic probe. Homology with tet(S) was detected in two clinical isolates of L. monocytogenes isolated in different geographical areas.
authors.affiliation: Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Charpentier
authors.forename: G
authors.initials: G
authors.lastname: Gerbaud
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D017000:Campylobacter coli / Q000187:drug effects / Q000235:genetics; D002352:Carrier Proteins; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000235:genetics; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics*; D026901:Membrane Transport Proteins; D008911:Minocycline / Q000494:pharmacology; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D011815:R Factors; D012336:RNA, Ribosomal, 16S; D015183:Restriction Mapping; D012270:Ribosomes / Q000187:drug effects; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D012336:RNA, Ribosomal, 16S; C083138:Tet M resistance protein, Bacteria; C083139:Tet O resistance protein, Bacteria; C083137:Tet(S) protein, Listeria monocytogenes; D013752:Tetracycline; D008911:Minocycline