Transformation by the fos or jun oncogene does not increase AP-1 DNA-binding activity.
The c-fos and c-jun proto-oncogenes encode components of the transcription factor AP-1. To determine whether transformation by the v-fos or v-jun oncogene results in alterations in the level or regulation of this factor, we have characterized AP-1 DNA-binding activity in nuclear extracts prepared from v-fos- and c-fos-transformed rat fibroblast cell lines and v-jun-transformed chicken embryo fibroblasts under various growth conditions. During proliferation, the level of AP-1 DNA-binding activity does not differ among the v-fos, c-fos, or v-jun-transformed cells and their normal progenitors, despite constitutive overexpression of the corresponding oncoproteins. Therefore, although necessary, it is not likely that an increase in DNA binding is sufficient for fos or jun transformation. Normal rat and chicken fibroblasts demonstrate very low levels of AP-1 DNA-binding activity when quiescent, and upon serum stimulation a biphasic increase is observed. A similar cyclical pattern is seen in v-fos-transformed cells, but in v-jun-transformed cells AP-1 DNA-binding activity does not fluctuate in response to serum stimulation, which suggests that this level of control may be exerted through the Jun component of the AP-1 complex.
Hawker
K L
KL
Cancer Research Campaign Laboratories, Beatson Institute for Cancer Research, Bearsden, Glasgow, United Kingdom.
Pintzas
A
A
Hennigan
R F
RF
Gillespie
D A
DA
Ozanne
B W
BW
D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005347:Fibroblasts; D016762:Genes, fos; D016758:Genes, jun; D007700:Kinetics; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016755:Proto-Oncogene Proteins c-jun; D012367:RNA, Viral; D013936:Thymidine