title: Simultaneous determination of a new anticancer drug galocitabine and its metabolites in blood by high-performance liquid chromatography.
abstract: A relatively simple and sensitive high-performance liquid chromatographic (HPLC) method is described for measuring galocitabine (Ro 09-1390) and its meatbolites, i.e. 5'-deoxy-fluorocytidine (5'-DFCR), 5'-deoxy-fluorouridine (5'-DFUR) and 5-fluorouracil (5-FU), in blood for the purpose of studying pharmacokinetics and toxicokinetics in small animals. The procedure for blood includes deproteinization with acetonitrile. Blood components were separated on a reversed-phase C18 column with a linear gradient of acetonitrile and water and detected at a wavelength of 270 nm. The between-day relative standard deviation (RSD) was less than 10% for all compounds at concentrations of 10-100 micrograms ml-1. The calibration curves obtained from the analysis of blood samples were linear and the correlation coefficients ranged from 0.997 to 0.999. The calculated determination limits were 6.9 micrograms ml-1 for galocitabine, 3.0 micrograms ml-1 for 5'-DFCR, 4.0 micrograms ml-1 for 5'-DFUR and 3.7 micrograms ml-1 for 5-FU.
authors.affiliation: Nippon Roche Research Center, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Funaki
authors.forename: H
authors.initials: H
authors.lastname: Onodera
authors.forename: K
authors.initials: K
authors.lastname: Ogawa
authors.forename: S
authors.initials: S
authors.lastname: Ichihara
authors.forename: H
authors.initials: H
authors.lastname: Fukazawa
authors.forename: I
authors.initials: I
authors.lastname: Kuruma
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity; D005467:Floxuridine / Q000097:blood; D005472:Fluorouracil / Q000097:blood; D007202:Indicators and Reagents; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D005467:Floxuridine; D003841:Deoxycytidine; C063993:Ro 09-1390; D005472:Fluorouracil; C025034:doxifluridine