title: Assessment of the "Amplicor" PCR test in the diagnosis of Chlamydia trachomatis infection.
abstract: We compared a commercially available PCR assay (Amplicor, Roche, Switzerland) and tissue culture isolation for the detection of C. trachomatis in urethral and/or endocervical swabs. Of the 200 patients studied (130 men and 70 women) PCR and tissue culture gave concordant results in 199 cases; in one case PCR was positive and culture negative. The Amplicor PCR assay proved fast and sensitive and suitable for routine use in most clinical microbiology laboratories.
authors.affiliation: Institute of Microbiology, University of Bologna St. Orsola Hospital, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Rumpianesi
authors.forename: M
authors.initials: M
authors.lastname: La Placa
authors.forename: A
authors.initials: A
authors.lastname: D'Antuono
authors.forename: M
authors.initials: M
authors.lastname: Negosanti
authors.forename: G
authors.initials: G
authors.lastname: Pavan
mesh_terms: D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D046508:Culture Techniques; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity