title: [The effect of nitroglycerin on the Ca2+ ion level in the cytoplasm of human lymphocytes].
abstract: Fluorescence was used to examine the effects of nitroglycerin (60 microM) on basal and mitogen-induced (ConA, 25 micrograms/ml) Ca2+ levels in the human lymphocytic cytoplasm. Calcium-blocking effect of nitroglycerin was markedly shown in the medium containing 1 mM CaCl2 and virtually absent in calcium-free buffer. It is concluded that nitroglycerin affects cellular Ca2+ entry. A 120-minute preincubation led to a complete loss of the inhibitory effects of the drug on mitogen-induced Ca2+ responses of lymphocytes. Human lymphocytes are proposed as an experimental model for investigating the effects of nitrates and the mechanisms of their tolerance development.
authors.forename: P V
authors.initials: PV
authors.lastname: Sergeev
authors.forename: G I
authors.initials: GI
authors.lastname: Storozhakov
authors.forename: E S
authors.initials: ES
authors.lastname: Khamitova
authors.forename: A S
authors.initials: AS
authors.lastname: Dukhanin
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Shilo
authors.forename: V E
authors.initials: VE
authors.lastname: Trepilets
mesh_terms: D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002478:Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D003593:Cytoplasm / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D004361:Drug Tolerance; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D005996:Nitroglycerin / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003208:Concanavalin A; D005996:Nitroglycerin; D002118:Calcium
vernacular_title: Vliianie nitroglitserina na soderzhanie ionov Ca2+ v tsitoplazme limfotsitov cheloveka.