110/140 laminin-binding protein immunoreactivity in spinal dorsal root ganglia: a capsaicin-insensitive reduction induced by constriction injury of the sciatic nerve in rats.
The distribution of 110/140 laminin-binding protein (110/140 LBP) in the spinal dorsal root ganglia (DRG) and its regulation by partial constriction of the sciatic nerve was studied in adult rats. The cross-sectional area of neurons with 110/140 LBP-immunoreactivity (-I) showed an approximately normal frequency distribution. The 110/140 LBP-I was observed in neuronal cell bodies exclusive of the nucleus. Following sciatic nerve constriction, the 110/140 LBP-I was downregulated in the ipsilateral L4-5 DRG. DRG neurons with a cross-sectional area > or = 1600 microns 2 were preferentially affected. Neonatal capsaicin-treatment, a procedure that selectively destroys a subpopulation of DRG neurons with fine unmyelinated axons, had no effect on the reduction of 110/140 LBP in the DRG induced by sciatic nerve constriction. Western immunoblot analysis confirmed a reduction of 110/140 LBP on the side ipsilateral to the constriction. These results demonstrate a LBP within primary sensory neurons and its suppression by peripheral nerve injury. The data support a role for LBP in the adult nervous system.
Ren
K
K
Neurobiology and Anesthesiology Branch, National Institutes of Health, Bethesda, Maryland.
Kibbey
M C
MC
Kleinman
H K
HK
Ruda
M A
MA
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D015153:Blotting, Western; D002211:Capsaicin / Q000494:pharmacology*; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005727:Ganglia, Spinal / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D017474:Receptors, Laminin / Q000276:immunology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries*
D002352:Carrier Proteins; D017474:Receptors, Laminin; D002211:Capsaicin