A microsatellite polymorphism in the human insulin receptor gene: a highly informative marker for linkage analysis.
To study genetic susceptibility to non-insulin dependent diabetes mellitus (NIDDM), association of insulin receptor gene, a candidate gene for NIDDM, with NIDDM was studied. A microsatellite polymorphism located in intron 2 of the human insulin receptor gene was detected by the polymerase chain reaction and used as a genetic marker. Eight different alleles were observed, indicating highly polymorphic nature of this marker. Although no association of this marker with NIDDM was observed in Japanese subjects, this marker will be useful for linkage studies of insulin receptor gene and genes located close to this gene on chromosome 19.
Ikegami
H
H
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Fukuda
M
M
Kawaguchi
Y
Y
Fujioka
Y
Y
Ueda
H
H
Ogihara
T
T
D000483:Alleles; D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D004276:DNA, Satellite / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011972:Receptor, Insulin / Q000235:genetics*; D012016:Reference Values
D004276:DNA, Satellite; D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides; D011972:Receptor, Insulin