Aromatic amino acid decarboxylase (AADC) immunohistochemistry in vertebrate brainstem with an antiserum raised against AADC made in E. coli.
Aromatic L-amino acid decarboxylase (AADC) is involved in the biosynthesis of catecholamines and indolamines. AADC is present in the nervous system, in the chromaffin cells, and in non-neuronal tissues. We tested the capacity of a new polyclonal antibody, obtained by immunization of rabbits with a recombinant protein beta-galactosidase-AADC, to detect monoaminergic neurons in the brainstem as well as monoaminergic paraneurons in the adrenal medulla from goldfish, frog, skink, quail, and mouse. In the adrenal gland we found an immunoreactivity that was consistent with the distributions of the chromaffin cells previously reported. In the brainstem, groups of immunoreactive neurons and several labelled fibers were observed in the five species studied. The raphe region showed cell bodies and processes similar to those previously identified as monoaminergic by other authors. In addition, in medulla oblongata and isthmic tegmentum we found, in goldfish, skink, and quail, neuronal groups similar to mammalian D groups which contain AADC but are devoided of serotonin and catecholamines.
Beltramo
M
M
Laboratoire de Cytologie, Institut des Neurosciences, URA CNRS 1488, Université Paris VI, France.
Krieger
M
M
Calas
A
A
Franzoni
M F
MF
Thibault
J
J
D000313:Adrenal Medulla / Q000201:enzymology; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000032:analysis* / Q000276:immunology; D003370:Coturnix; D004926:Escherichia coli / Q000201:enzymology*; D005260:Female; D006054:Goldfish; D007106:Immune Sera; D007150:Immunohistochemistry; D008116:Lizards; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011893:Rana esculenta; D011903:Raphe Nuclei / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D014714:Vertebrates / Q000378:metabolism*
D007106:Immune Sera; D011994:Recombinant Proteins; D001142:Aromatic-L-Amino-Acid Decarboxylases