Mitomycin C stimulates the expression of human immunodeficiency virus long terminal repeat sequences in rat and human fibroblasts.
We have employed a recombinant plasmid, pBHIV1, carrying the long terminal repeat (LTR) sequences of the human immunodeficiency virus-1 (HIV-1) linked to the reporter chloramphenicol acetyl transferase (CAT) gene and to the aminoglycoside phosphotransferase (aph) gene as a selectable marker. We have introduced pBHIV1 into rat 208F and human MRCSV40TGR fibroblasts and obtained stable geneticin resistant RFBHIV1-1 and SVTGHIV1-1 cells, respectively. Both transfectant cells express CAT activity from the HIV LTR promoter. The response to the antineoplastic drug mitomycin C was studied on the LTR regulated CAT activity in both cell lines. It was found that mitomycin C at 10 micrograms/mL concentration stimulates the expression of CAT from the HIV LTR 77-fold in rat RFBHIV1-1 and 3.1-fold in human SVTGHIV1-1 cells.
Zoumpourlis
V
V
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Spandidos
D A
DA
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism*; D015870:Gene Expression; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology* / Q000633:toxicity; D010957:Plasmids; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D016685:Mitomycin; D015500:Chloramphenicol O-Acetyltransferase