Colony immunoblot assay of botulinal toxin.
Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin was transferred from the agar medium to a nitrocellulose support and the immobilized toxin was probed with type-specific antibodies. The method identified the toxin types of the colonies grown from a mixed inoculum of C. botulinum serotypes. The specificity of the antitoxins for type A and B toxins was improved by adsorption of the antitoxins with the antigens of heterologous type cultures.
Goodnough
M C
MC
Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
Hammer
B
B
Sugiyama
H
H
Johnson
E A
EA
D000918:Antibody Specificity; D001905:Botulinum Toxins / Q000032:analysis*; D003013:Clostridium / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015151:Immunoblotting
D001905:Botulinum Toxins