Development of primary culture of ovine fetal hepatocytes for studies of amino acid metabolism and insulinlike growth factors.
We report the development and characterization of a system of primary culture of ovine fetal hepatocytes to aid in the understanding of the cellular regulation of fetal growth and metabolism with emphasis on amino acid metabolism and insulinlike growth factor gene expression and to allow comparison to in vivo studies. Hepatocytes were isolated from late gestation fetal lambs by in situ perfusion and collagenase digestion utilizing occlusion of the ductus venosus to limit intrahepatic shunting. Hepatocytes were cultured in media modified to mimic fetal concentrations of glucose, lactate, and amino acids. Ovine fetal hepatocytes in primary culture maintain the pattern of fetal amino acid production and utilization seen across the fetal liver in vivo. Specifically, there is a net production of serine and a net utilization of glycine. Cultured ovine fetal hepatocytes specifically increase tritiated thymidine incorporation in response to insulin and insulinlike growth factor II (IGF-II). IGF-II mRNA abundance is high and IGF-I mRNA is low in cultured ovine fetal hepatocytes as in the fetal sheep liver in vivo. These data demonstrate the successful isolation of ovine fetal hepatocytes that retain some of the characteristics of the ovine fetal liver while maintained in short-term culture.
Townsend
S F
SF
Department of Pediatrics, University of Colorado Health Sciences Center, Denver 80262.
Thureen
P J
PJ
Hay
W W
WW
Narkewicz
M R
MR
D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D007773:Lactates / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D012756:Sheep / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism
D000596:Amino Acids; D007773:Lactates; D012333:RNA, Messenger; D012694:Serine; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D004247:DNA; D005947:Glucose; D005998:Glycine; D013936:Thymidine