cDNA cloning and expression of a potato (Solanum tuberosum) invertase.
A cDNA clone encoding an invertase isoenzyme has been isolated from a potato leaf cDNA library. The deduced amino acid sequence shows significant similarities to previously characterised invertases. The highest degree of overall similarity, including the signal peptide sequence, is to carrot cell wall invertase, suggesting that the potato gene encodes an apoplastic enzyme. Expression of the gene, as determined by RT-PCR, is detected in stem and leaf tissue, and at lower levels in tuber, but is absent from roots.
Hedley
P E
PE
Scottish Crop Research Institute, Dundee, UK.
Machray
G C
GC
Davies
H V
HV
Burch
L
L
Waugh
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*; D043324:beta-Fructofuranosidase
D007527:Isoenzymes; D004247:DNA; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase