Transgenic potato plants overexpressing the pathogenesis-related STH-2 gene show unaltered susceptibility to Phytophthora infestans and potato virus X.
The STH-2 gene is rapidly activated in potato leaves and tubers following elicitation or infection by Phytophthora infestans. However, its biochemical function remains unknown. In order to ascertain if STH-2 protein is directly involved in the defense of potato against pathogens, the STH-2 coding sequence under the control of the CaMV 35S promoter was introduced into potato plants. Transgenic plants expressing the STH-2 gene were analyzed for an altered pattern of susceptibility to a compatible race of P. infestans and to potato virus X. Results indicate that constitutive expression of the STH-2 gene did not reduce susceptibility of potato to these pathogens.
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Department of Biochemistry, Université de Montréal, Canada.
Bertrand
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C
Brisson
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N
D003001:Cloning, Molecular; D017343:Genes, Plant; D020022:Genetic Predisposition to Disease; D015151:Immunoblotting; D010838:Phytophthora / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics; D010942:Plant Viruses / Q000472:pathogenicity*; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
D010940:Plant Proteins