title: Escherichia coli produces linoleic acid during late stationary phase.
abstract: Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linoleic acid methyl ester was identified by its mass spectrum. Lipids extracted from late-stationary-phase cells generated thiobarbituric acid-reactive carbonyl products when incubated with a free radical initiator. In contrast, extracts from log-phase or early-stationary-phase cells failed to do so, in accordance with the presence of polyunsaturated fatty acid only in the stationary-phase cells.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: H D
authors.initials: HD
authors.lastname: Rabinowitch
authors.forename: D
authors.initials: D
authors.lastname: Sklan
authors.forename: D H
authors.initials: DH
authors.lastname: Chace
authors.forename: R D
authors.initials: RD
authors.lastname: Stevens
authors.forename: I
authors.initials: I
authors.lastname: Fridovich
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D008041:Linoleic Acids; D019787:Linoleic Acid; D005947:Glucose