title: Effect of an n-3 fatty acid-deficient diet on the adenosine-dependent melatonin release in cultured rat pineal.
abstract: We studied the effect of a diet deficient in n-3 fatty acids on the adenosine-dependent melatonin release from cultured rat pineal gland after stimulation by 5'-N-ethylcarboxamidoadenosine (NECA), an A2 adenosine agonist. Experiments were conducted with 2-month-old rats raised on semipurified diets containing either peanut oil (n-3 deficients) or peanut plus rapeseed oil (controls). The proportion of docosahexaenoic acid (22:6 n-3) in the pineal total lipid fraction and in phosphatidylcholine and phosphatidylethanolamine was significantly decreased in n-3-deficient rats. This was compensated for partially by an increase in 22:4 n-6 and 22:5 n-6 levels. The activity of the cultured rat pineal, in terms of cyclic AMP content and N-acetylserotonin and melatonin release in the medium, was lower after stimulation by 10(-5) mol/L NECA in the group fed peanut oil than in the group fed peanut plus rapeseed oil. The increased ratio of n-6/n-3 fatty acids in pineal total lipids and the major glycerophospholipids (phosphatidylcholine and phosphatidylethanolamine) may have an important influence on the rat pineal responses. The results are discussed in the context of changes in membrane-bound proteins, including enzymes and/or receptors involved in the rat pineal gland function.
authors.affiliation: INSERM U. 352, Laboratoire de Chimie Biologique, INSAL, Villeurbanne, France.
authors.forename: N
authors.initials: N
authors.lastname: Gazzah
authors.forename: A
authors.initials: A
authors.lastname: Gharib
authors.forename: I
authors.initials: I
authors.lastname: Delton
authors.forename: P
authors.initials: P
authors.lastname: Molière
authors.forename: G
authors.initials: G
authors.lastname: Durand
authors.forename: R
authors.initials: R
authors.lastname: Christon
authors.forename: M
authors.initials: M
authors.lastname: Lagarde
authors.forename: N
authors.initials: N
authors.lastname: Sarda
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004041:Dietary Fats / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005260:Female; D008550:Melatonin / Q000378:metabolism*; D009924:Organ Culture Techniques; D010743:Phospholipids / Q000378:metabolism; D010870:Pineal Gland / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D010743:Phospholipids; D012701:Serotonin; D008550:Melatonin; D000241:Adenosine; C006389:N-acetylserotonin