Partial characterization of HCl heat extracted antigen derived from Fusobacterium necrophorum.
The HCl heat extracted antigen derived from Fusobacterium necrophorum was partially purified by CM cellulose column chromatography and treatment by freeze-thawing. It demonstrated relatively high enzyme-linked immunosorbent assay values to anti-F. necrophorum sera. In sodium dodecyl sulphate polyacrylamide gel electrophoresis, the partially purified preparation exhibited one definite and two faint bands. The approximate molecular weight of the former was estimated as 16 kD.
Kanoe
M
M
Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
Matsuoka
Y
Y
Kai
K
K
D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D005675:Fusobacterium necrophorum / Q000276:immunology*; D006358:Hot Temperature; D006851:Hydrochloric Acid
D000942:Antigens, Bacterial; D006851:Hydrochloric Acid