title: Effects of halothane anesthesia on the motility of the sphincter of Oddi and transsphincteric flow in Australian brush-tailed possums.
abstract: We evaluated the effect of halothane anesthesia on the motility of the sphincter of Oddi and simultaneous transsphincteric flow in Australian Brush-tailed possums (Trichosurus vulpecula). Halothane levels in the range of 0.25 to 2% were administered and decreased transsphincteric flow in a dose-dependent manner. Sphincter of Oddi basal pressure was higher than normal, but not in a dose-dependent manner. Additionally, halothane anesthesia influenced the sphincter of Oddi motility by decreasing the motility index (mean amplitude multiplied by frequency of contractions). This decrease was dose dependent. These findings indicate that sphincter of Oddi basal pressure is a major component of sphincter of Oddi motility responsible for regulating transsphincteric flow in this species. For studies of the sphincter of Oddi motility in anesthetized Australian Brush-tailed possums, we recommend anesthetic induction with ketamine (50 mg/kg, i.m.) and the inspired halothane level should not exceed 0.75% during the study period, as the effects we have demonstrated were most evident at levels greater than 0.75%. If higher halothane levels are required to maintain satisfactory anesthesia, an alternative anesthetic agent should be considered.
authors.affiliation: Department of Surgery, Flinders Medical Centre, Bedford Park, South Australia.
authors.forename: Y F
authors.initials: YF
authors.lastname: Liu
authors.forename: G T
authors.initials: GT
authors.lastname: Saccone
authors.forename: R A
authors.initials: RA
authors.lastname: Baker
authors.forename: J R
authors.initials: JR
authors.lastname: Harvey
authors.forename: J
authors.initials: J
authors.lastname: Toouli
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D006221:Halothane / Q000494:pharmacology*; D008297:Male; D009893:Opossums / Q000502:physiology*; D012039:Regional Blood Flow / Q000187:drug effects; D009803:Sphincter of Oddi / Q000098:blood supply / Q000187:drug effects* / Q000502:physiology
chemical_list: D006221:Halothane