4-(Trifluoromethyl)-2,3,5,6-tetrafluorobenzyl bromide as a new electrophoric derivatizing reagent.
4-(Trifluoromethyl)-2,3,5,6-tetrafluorobenzyl bromide (TTBB) was synthesized in a single step from alpha,alpha,alpha,2,3,5,6-heptafluoro-p-xylene. The purpose of TTBB is to function as an analogue of pentafluorobenzyl bromide (PFBB) in electrophoric derivatization reactions prior to detection by gas chromatography-electron-capture negative ion mass spectrometry (GC-ECNI-MS). In more detail, it was anticipated that TTBB could be used along with, or as a substitute for, PFBB to help control some interferences and confirm results. This is because a TTBB-product (of an analyte) would have different retention and sometimes m/z characteristics than a corresponding PFBB product in GC-ECNI-MS, while the two products should be similar in their ease of formation and yields. Results demonstrating these expectations were achieved by derivatizing and detecting two analytes with these reagents: N7-(2-hydroxyethyl)xanthine, and 2,3-pyrenedicarboxylic acid.
Saha
M
M
Department of Pharmaceutical Sciences, Bouve Pharmacy and Health Sciences, Northeastern University, Boston, MA 02115.
Saha
J
J
Giese
R W
RW
D005464:Fluorobenzenes / Q000138:chemical synthesis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007202:Indicators and Reagents; D011721:Pyrenes / Q000032:analysis / Q000737:chemistry; D014970:Xanthines / Q000032:analysis / Q000737:chemistry; D005466:Fluorocarbons
D005464:Fluorobenzenes; D007202:Indicators and Reagents; D011721:Pyrenes; D014970:Xanthines; C025063:pentafluorobenzyl bromide; D005466:Fluorocarbons; C083052:4-(trifluoromethyl)-2,3,5,6-tetrafluorobenzyl bromide