Structure and regulation of the gene encoding the neuron-specific protein PEP-19.
PEP-19 is a 61 amino acid polypeptide that is localized to neurons. PEP-19 is translated from a 0.6 kb poly(A)+ RNA; however, the gene from which it is transcribed spans more than 30 kbp and comprises three exons and two large introns. Exon 1 contains the 5'-untranslated region of PEP-19 and the first three amino acids of the coding sequence; exon 2 codes for the next 17 amino acids while the majority of the PEP-19 mRNA, comprising the remaining 42 amino acids and the 3'-untranslated region, is in exon 3. Gel retardation analysis identifies a region of DNA in the putative promoter of PEP-19 that binds preferentially to nuclear extracts from cerebellum. However, constructs containing 1.35 kbp of 5'-upstream genomic DNA of PEP-19 fused to lacZ do not express in transgenic mice, suggesting that intragenic DNA may be essential for the regulation of PEP-19.
Sangameswaran
L
L
Department of Neuroscience, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Morgan
J I
JI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002148:Calmodulin-Binding Proteins; D002467:Cell Nucleus / Q000378:metabolism; D003062:Codon; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D015698:Genomic Library; D007438:Introns; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics*; D009474:Neurons / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D002148:Calmodulin-Binding Proteins; D003062:Codon; D009419:Nerve Tissue Proteins; D009838:Oligodeoxyribonucleotides; C489091:Pcp4 protein, mouse; C489090:Pcp4 protein, rat; D011993:Recombinant Fusion Proteins; D004247:DNA; D001616:beta-Galactosidase