title: Transcription factors are required for the meiotic recombination hotspot at the HIS4 locus in Saccharomyces cerevisiae.
abstract: The full activity of a recombination initiation site located 5' of HIS4 requires the binding of the transcription factors RAP1, BAS1, and BAS2. Two RAP1 binding sites can substitute for the wild-type initiation site. A 51-bp region of telomeric DNA inserted upstream of either HIS4 or ARG4 very strongly stimulates recombination. We suggest that the ability of transcription factors to induce recombination is a consequence of an altered chromatin structure that favors the entry of proteins that initiate recombination, rather than an effect of these factors on transcription.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
authors.forename: M A
authors.initials: MA
authors.lastname: White
authors.forename: M
authors.initials: M
authors.lastname: Dominska
authors.forename: T D
authors.initials: TD
authors.lastname: Petes
mesh_terms: D001483:Base Sequence; D002843:Chromatin / Q000648:ultrastructure; D003433:Crosses, Genetic; D004252:DNA Mutational Analysis; D005656:Fungal Proteins / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D018398:Homeodomain Proteins; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D016615:Telomere; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D020668:rap GTP-Binding Proteins
chemical_list: C061767:BAS1 protein, S cerevisiae; D002843:Chromatin; D005656:Fungal Proteins; D018398:Homeodomain Proteins; C051611:PHO2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins