Prenatal expression of the 65-kDa heat-shock protein homologue in pig tissues.
A monoclonal antibody (ML30) recognizing the 65-kDa heat-shock protein of mycobacteria and reacting with homologous human protein was found to stain various porcine tissues. Development of this reactivity was studied. The first ML30-positive cells were embryonic hepatocytes. The protein reacting with the ML30 antibody was localized predominantly in the Golgi area and mitochondria of hepatocytes. Cell membranes of some peripheral blood lymphocytes were also found to bind ML30.
Trebichavský
I
I
Department of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Prague.
Splíchal
I
I
Ivanyi
J
J
Tlaskalová
H
H
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000196:embryology / Q000378:metabolism; D013552:Swine
D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins