title: An immunohistological study of cutaneous lymphocytes in vitiligo.
abstract: In a study of 49 biopsies from the margins of depigmented cutaneous lesions in 18 patients with vitiligo, highly significant overall increases were found in CD3+, CD4+, and CD8+ T cells, though cell numbers in individual cases were often within the normal range. Many of the T cells were activated (MHC class II+, interferon gamma+), of CD45RO (UCHL1+) memory subset, and many expressed the cutaneous lymphocyte-associated antigen (HECA-452+) typical of skin-homing T cells. Immunohistologically, the most intense epidermal T-cell infiltration was present within 0.6 mm of the edge of the lesion in 10 of 13 double-stained sections with a clearly defined zone of melanocyte depletion. In 40 lesions from 17 patients seen 11-64 weeks after biopsy, no apparent association was found between T-cell numbers and disease activity as assessed by Köbnerization of biopsy wounds or spread of depigmentation. These findings are consistent with the hypothesis that lesional T cells rather than circulating antimelanocytic antibody may be responsible for the supposedly autoimmune but characteristically patchy destruction of cutaneous melanocytes in vitiligo. Nevertheless, many of the infiltrating T cells are probably innocent bystanders attracted by upregulated cell adhesion molecules near sites of melanocyte damage.
authors.affiliation: Department of Pathology, Royal Infirmary, Glasgow, U.K.
authors.forename: A M
authors.initials: AM
authors.lastname: Badri
authors.forename: P M
authors.initials: PM
authors.lastname: Todd
authors.forename: J J
authors.initials: JJ
authors.lastname: Garioch
authors.forename: J E
authors.initials: JE
authors.lastname: Gudgeon
authors.forename: D G
authors.initials: DG
authors.lastname: Stewart
authors.forename: R B
authors.initials: RB
authors.lastname: Goudie
mesh_terms: D001706:Biopsy; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D008544:Melanocytes / Q000473:pathology; D012867:Skin / Q000276:immunology* / Q000293:injuries; D013601:T-Lymphocytes / Q000276:immunology*; D014820:Vitiligo / Q000276:immunology* / Q000473:pathology
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens