title: [Immunoreactivity of synthetic peptides, corresponding to B-cellular epitopes of human type I T-lymphotrophic virus structural proteins].
abstract: The immunoreactivity of 25 synthetic peptides corresponding to amino acid fragments of the HTLV-I structural proteins p19 gag, gp46 and gp21 env were studied in enzyme linked immunosorbent assay using a serum panel of 70 reference positive specimens with anti-HTLV-I antibodies. The location of the synthetic peptides containing the B-cell epitopes of HTLV-I was established. Anti-HTLV-I antibodies effectively recognized these peptides. The significance of some amino acids for forming the HTLV-I antigenic determinants was estimated. The synthetic peptides with amino acid sequences 100-130 p19 gag and 176-201 gp46 env were found to have most immunoreactivity (90-99% recognition by sera of HTLV-I infected patients) and mimic the immunodominant B-cell epitopes of HTLV-I structural proteins.
authors.forename: A M
authors.initials: AM
authors.lastname: Rasuli
authors.forename: N N
authors.initials: NN
authors.lastname: Klepikov
authors.forename: S M
authors.initials: SM
authors.lastname: Andreev
authors.forename: M V
authors.initials: MV
authors.lastname: Sidorova
authors.forename: M G
authors.initials: MG
authors.lastname: Vafina
authors.forename: N B
authors.initials: NB
authors.lastname: Seniuta
authors.forename: O A
authors.initials: OA
authors.lastname: Pavlish
authors.forename: A V
authors.initials: AV
authors.lastname: Syrtsev
authors.forename: V E
authors.initials: VE
authors.lastname: Gurtsevich
mesh_terms: D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015481:HTLV-I Antibodies / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D015481:HTLV-I Antibodies; D016056:Immunodominant Epitopes; D010455:Peptides; D015678:Viral Structural Proteins
vernacular_title: Immunoreaktivnost' sinteticheskikh peptidov, sootvetstvuiushchikh B-kletochnym épitopam strukturnykh belkov T-limfotropnogo virusa cheloveka I tipa.