title: Deoxyribocytidine is salvaged not only into DNA but also into phospholipid precursors. IV. Exogenous deoxyribocytidine can be used with the same efficacy as (ribo)cytidine for lipid activation.
abstract: Utilisation of exogenous (ribo)cytidine (3H-CR) and deoxyribocytidine (3H-CdR) for DNA/RNA synthesis and for activation of phospholipid intermediates was compared in human tonsillar lymphocytes. Incorporation of 3H-CdR into dCDP-choline and into dCDP-DAG was similar or even higher than labelling of CDP-choline and CDP-DAG from 3H-CR. No interconversion was found between CDP-DAG and dCDP-DAG, as shown by TLC separation of the ribo- and deoxyribocytidine derivatives. Moreover, a strict separation was found between the salvage pathways of deoxyribocytidine and (ribo)cytidine, as 4000-fold excess of non labelled (ribo)cytidine did not show any specific effect on 3H-dCDP-DAG labelling from exogenous 3H-CdR.
authors.affiliation: 1st Institute of Biochemistry, Semmelweis Medical University, Budapest, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Sasvári-Székely
authors.forename: T
authors.initials: T
authors.lastname: Spasokukotskaja
authors.forename: M
authors.initials: M
authors.lastname: Staub
mesh_terms: D002478:Cells, Cultured; D003562:Cytidine / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D003841:Deoxycytidine / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D008954:Models, Biological; D014066:Palatine Tonsil; D010743:Phospholipids / Q000096:biosynthesis* / Q000302:isolation & purification; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
chemical_list: D010743:Phospholipids; D003841:Deoxycytidine; D014316:Tritium; D003562:Cytidine; D004247:DNA; D013936:Thymidine