The PKC-inhibitor Ro 31-8220 selectively suppresses PMA- and diacylglycerol-induced fluid pinocytosis and actin polymerization in PMNs.
The PKC-inhibitor Ro 31-8220 inhibits stimulated fluid pinocytosis of human PMNs induced by the PKC-activators phorbol myristate acetate (PMA, IC50 = 1.35 x 10(-6) M) or diacylglycerols (OAG, diC8) by 95%, whereas Ro 31-8220 has no effect on D2O- or fNLPNTL-induced pinocytosis and enhances cytochalasin D- induced pinocytosis. Also formation of F-actin induced by PMA or diacylglycerols is selectively inhibited. The results indicate that a Ro 31-8220 -sensitive PKC is involved in signal transduction for enhanced pinocytosis and F-actin formation in response to one class of stimuli (classical activators of PKC) but not to others.
Keller
H U
HU
Institute of Pathology, University of Bern, Switzerland.
Niggli
V
V
D000199:Actins / Q000378:metabolism*; D015638:Cytochalasin D / Q000494:pharmacology; D004075:Diglycerides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology; D010873:Pinocytosis / Q000187:drug effects*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000199:Actins; D004075:Diglycerides; D007211:Indoles; D009842:Oligopeptides; D015638:Cytochalasin D; C021642:F-chemotactic peptide; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; C064758:Ro 31-8220