Genotypic and phenotypic characterization of HIV-1 patients with primary infection.
Better characterization of human immunodeficiency virus-type 1 (HIV-1) in patients with primary infection has important implications for the development of an acquired immunodeficiency syndrome (AIDS) vaccine because vaccine strategies should target viral isolates with the properties of transmitted viruses. In five HIV-1 seroconverters, the viral phenotype was found to be uniformly macrophage-tropic and non-syncytium-inducing. Furthermore, the viruses were genotypically homogeneous within each patient, but a common signature sequence was not discernible among transmitted viruses. In the two cases where the sexual partners were also studied, the sequences of the transmitted viruses matched best with minor variants in the blood of the transmitters. There was also a stronger pressure to conserve sequences in gp120 than in gp41, nef, and p17, suggesting that a selective mechanism is involved in transmission.
Zhu
T
T
Aaron Diamond AIDS Research Center, New York University School of Medicine, NY 10016.
Mo
H
H
Wang
N
N
Nam
D S
DS
Cao
Y
Y
Koup
R A
RA
Ho
D D
DD
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D005260:Female; D015683:Gene Products, gag / Q000737:chemistry / Q000235:genetics; D005814:Genes, Viral; D005838:Genotype; D015726:Giant Cells / Q000502:physiology; D015488:HIV Antigens / Q000737:chemistry / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000235:genetics*; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000235:genetics; D015658:HIV Infections / Q000382:microbiology* / Q000635:transmission; D006679:HIV Seropositivity / Q000382:microbiology; D015497:HIV-1 / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D008264:Macrophages; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D016415:Sequence Alignment; D012747:Sexual Partners; D014764:Viral Proteins; D014779:Virus Replication; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1