A novel hinge system and incubation chamber for emulsion-coated coverslip autoradiography.
We describe an emulsion-coated coverslip autoradiographic technique for large (50 x 50 mm) sections of monkey or human brain. The technique uses adhesive-backed, teflon-reinforced aluminum foil as a flexible hinge that allows the coverslip to swing away from the slide so that the emulsion and tissue can be processed independently. We also describe a plexiglas incubation chamber that allows two slides with coverslips folded away to be processed back-to-back in 5 ml of reagent solution. In general use, the chamber minimizes the volume of solution needed to cover large slide-mounted sections and is ideal for radioligand or immunohistochemical reactions that use expensive reagents. For autoradiography, the chamber greatly facilitates independent processing of the emulsion and tissue section. Together, the foil-hinge method and incubation chamber virtually eliminate the major technical pitfalls of previous emulsion-coated coverslip methods. Moreover, they facilitate combination of histochemical or immunocytochemical staining with autoradiographic localization of neurotransmitter receptors in the same tissue section. This is demonstrated by thioflavin S staining of neuritic plaques and tangles and autoradiographic localization of serotonin 1A receptors in sections of Alzheimer disease brain.
Rhodes
K J
KJ
Dept. of Anatomy and Neurobiology, Boston U. School of Medicine, MA 02118.
Meiners
K A
KA
Rosene
D L
DL
D000818:Animals; D001345:Autoradiography / Q000295:instrumentation* / Q000379:methods*; D052160:Benzothiazoles; D001923:Brain Chemistry; D003487:Cyanoacrylates; D004655:Emulsions; D005453:Fluorescence; D006652:Histological Techniques; D006801:Humans; D007185:Incubators; D008253:Macaca mulatta; D011950:Receptors, Cholinergic / Q000032:analysis; D011985:Receptors, Serotonin / Q000032:analysis; D013844:Thiazoles
D052160:Benzothiazoles; D003487:Cyanoacrylates; D004655:Emulsions; D011950:Receptors, Cholinergic; D011985:Receptors, Serotonin; D013844:Thiazoles; C009462:thioflavin T; C040229:MK 6 cyanoacrylate