title: Coexpression of neurotrophins and their receptors in neurons of the central nervous system.
abstract: Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are neuronal survival molecules which utilize the Trk family of tyrosine kinase receptors. Using double-label in situ hybridization, we demonstrate that mRNAs for BDNF and its high-affinity receptor TrkB are coexpressed in hippocampal and cortical neurons. Also, a large number of neurons in these areas coexpress NGF and BDNF mRNAs. Epileptic seizures lead to increased levels of both BDNF/TrkB and NGF/BDNF mRNAs in double-labeled cells. Our results show that individual neurons of the central nervous system can coexpress neurotrophins and their receptors and produce two neurotrophic factors. These factors could support neuronal survival after brain insults, not only via retrograde transport but also through autocrine mechanisms.
authors.affiliation: Department of Neurology, University Hospital, Lund, Sweden.
authors.forename: Z
authors.initials: Z
authors.lastname: Kokaia
authors.forename: J
authors.initials: J
authors.lastname: Bengzon
authors.forename: M
authors.initials: M
authors.lastname: Metsis
authors.forename: M
authors.initials: M
authors.lastname: Kokaia
authors.forename: H
authors.initials: H
authors.lastname: Persson
authors.forename: O
authors.initials: O
authors.lastname: Lindvall
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry; D019208:Brain-Derived Neurotrophic Factor; D002540:Cerebral Cortex / Q000737:chemistry* / Q000166:cytology; D006624:Hippocampus / Q000737:chemistry* / Q000166:cytology; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification*; D009414:Nerve Growth Factors / Q000302:isolation & purification*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D009474:Neurons / Q000737:chemistry; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D020801:Receptor, Ciliary Neurotrophic Factor; D012640:Seizures
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D008565:Membrane Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor