title: Structural studies of the saccharide part of the cell envelope lipopolysaccharide from Haemophilus influenzae strain AH1-3 (lic3+).
abstract: The structure of the saccharide part of the lipopolysaccharide from Haemophilus influenzae strain AH1-3 (lic3+) has been investigated. The saccharide was obtained from the lipopolysaccharide by mild acid hydrolysis followed by high-performance anion-exchange chromatography, and isolated fractions were studied by methylation analysis, NMR spectroscopy, and FAB mass spectrometry. The major saccharide is a heptasaccharide with the following structure, [formula: see text] in which Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid and PEA is 2-aminoethyl phosphate. Hep is identified as L-glycero-D-manno-heptose. The absolute configuration of the phosphorylated heptose is tentative only.
authors.affiliation: Karolinska Institute, Department of Clinical Bacteriology, Huddinge Hospital, Sweden.
authors.forename: E K
authors.initials: EK
authors.lastname: Schweda
authors.forename: O E
authors.initials: OE
authors.lastname: Hegedus
authors.forename: S
authors.initials: S
authors.lastname: Borrelli
authors.forename: A A
authors.initials: AA
authors.lastname: Lindberg
authors.forename: J N
authors.initials: JN
authors.lastname: Weiser
authors.forename: D J
authors.initials: DJ
authors.lastname: Maskell
authors.forename: E R
authors.initials: ER
authors.lastname: Moxon
mesh_terms: D002240:Carbohydrate Sequence; D006193:Haemophilus influenzae / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D006539:Heptoses / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D006539:Heptoses; D008070:Lipopolysaccharides; D009844:Oligosaccharides