Nucleosides and nucleotides. CXIX. Inhibition of DNA-cytosine methylase HhaI by a self-complementary oligonucleotide containing 5-fluorocytosine.
A self-complementary decadeoxyribonucleotide, 5'd(GAAGFGCTTC)3', containing 5-fluorocytosine (F) in substitution for cytosine at the methylation site of DNA-cytosine methylase HhaI (MHhaI) has been synthesized. MHhaI was inhibited by the pre-incubation of the enzyme with d(GAAGFGCTTC).
Ono
A
A
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Matsuo
Y
Y
Matsuda
A
A
Ueda
T
T
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis / Q000494:pharmacology*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors*; D015257:DNA-Cytosine Methylases / Q000037:antagonists & inhibitors*; D005437:Flucytosine / Q000494:pharmacology*; D006358:Hot Temperature; D006868:Hydrolysis; D008745:Methylation; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D015183:Restriction Mapping
D009838:Oligodeoxyribonucleotides; D004247:DNA; D005437:Flucytosine; C037664:DNA modification methylase HhaI; D015257:DNA-Cytosine Methylases; D004248:DNA (Cytosine-5-)-Methyltransferases