title: Characterization of Salmonella california and S. typhimurium strains with reduced ability to colonize the intestinal tract of broiler chicks.
abstract: This study determined the ability of eight strains of Salmonella and their agarsubcultured variants to colonize the intestinal tract of broiler chicks. Nalidixic-acid (NAL)-resistant and streptomycin-resistant subcultured strains (S. california 1989/A and S. typhimurium 3366/A) that persisted in the ceca of chicks in lower numbers than their NAL-resistant parent strains (1989/O and 3366/O) were selected for additional study S. typhimurium strain 3366/A was present in the ceca of chicks in lower numbers than the parent strain 3366/O when given concomitantly with the parent strain or when the two strains were given separately to different chicks. S. california 1989/A strain was present in the ceca in lower numbers than the parent strain after concomitant oral or intracloacal inoculation. Strains 3366/O and 3366/A of S. typhimurium differed in growth rates in BHI broth and cecal mucus. The lipopolysaccharide (LPS) profile indicated that LPS components present in S. california 1989/O were missing from strain 1989/A. A mutant of 1989/O--2095/R--was also LPS- and colonization-deficient.
authors.affiliation: Poultry Microbiological Safety Research Unit, R. B. Russell Agricultural Research Center, U.S. Department of Agriculture, Athens, Georgia 30613.
authors.forename: S E
authors.initials: SE
authors.lastname: Craven
authors.forename: N A
authors.initials: NA
authors.lastname: Cox
authors.forename: J S
authors.initials: JS
authors.lastname: Bailey
authors.forename: N J
authors.initials: NJ
authors.lastname: Stern
authors.forename: R J
authors.initials: RJ
authors.lastname: Meinersmann
authors.forename: L C
authors.initials: LC
authors.lastname: Blankenship
mesh_terms: D000818:Animals; D002432:Cecum / Q000382:microbiology*; D002465:Cell Movement; D002645:Chickens / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000662:veterinary; D008070:Lipopolysaccharides / Q000032:analysis; D011201:Poultry Diseases / Q000382:microbiology*; D012475:Salmonella / Q000737:chemistry / Q000254:growth & development*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000737:chemistry / Q000254:growth & development*; D013045:Species Specificity
chemical_list: D008070:Lipopolysaccharides