title: Preliminary crystallographic studies on scytalone dehydratase from Magnaporthe grisea.
abstract: Magnaporthe grisea are pathogenic, directly penetrating fungi which cause rice blast disease. Isolated, non-pathogenic mutant strains which are defective in the biosynthesis of dihydroxynapthalene-derived melanin fail to infect host plants and have been shown to lack certain key enzymes in melanin biosynthesis. One such enzyme is scytalone dehydratase that converts scytalone to 1,3,8-trihydroxy-naphthalene. Crystallization trials of scytalone dehydratase were undertaken with the expectation that structural information on this enzyme would facilitate design of high affinity inhibitors which might find use in the control of rice blast disease. We now report that recombinant scytalone dehydratase, complexed with a tight binding inhibitor, has been crystallized with PEG 4000 as a precipitant. The crystals are trigonal and belong to the space group P321 with the cell dimension: a = b = 75.5 A, c = 73.8 A. The observed diffraction extends to 2.5 A. Analysis of the packing in the cell suggests that scytalone dehydratase forms a symmetric trimer. These results are consistent with sedimentation equilibrium experiments indicating that the solution aggregation state of scytalone dehydratase was trimeric over a 24,000-fold concentration range.
authors.affiliation: Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: T
authors.initials: T
authors.lastname: Lundqvist
authors.forename: P C
authors.initials: PC
authors.lastname: Weber
authors.forename: C N
authors.initials: CN
authors.lastname: Hodge
authors.forename: E H
authors.initials: EH
authors.lastname: Braswell
authors.forename: J
authors.initials: J
authors.lastname: Rice
authors.forename: J
authors.initials: J
authors.lastname: Pierce
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D003460:Crystallization; D006836:Hydro-Lyases / Q000737:chemistry*; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D006836:Hydro-Lyases; C082741:scytalone dehydratase