title: Ascorbate-dependent elevation of mRNA levels for cytochrome P450s induced by polychlorinated biphenyls.
abstract: Trichlorobiphenyl induced only CYP1A2 mRNA, while pentachlorobiphenyl induced both CYP1A2 and CYP2B1 mRNAs in rat liver. The mRNA levels for these P450s were elevated when ascorbic acid-deficient ODS rats (mutant rats with a hereditary osteogenic disorder) were fed a diet supplemented with ascorbic acid. The amount of CYP2B1 mRNA increased rapidly and reached a maximum level of approximately double within 24 hr of injection of pentachlorobiphenyl. Thereafter, the amount of its mRNA decreased to a steady level. This pattern was roughly paralleled by changes in the amount of CYP1A2 mRNA.
authors.affiliation: Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Torii
authors.forename: K
authors.initials: K
authors.lastname: Hitomi
authors.forename: N
authors.initials: N
authors.lastname: Tsukagoshi
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis / Q000494:pharmacology; D001206:Ascorbic Acid Deficiency / Q000235:genetics*; D001848:Bone Diseases, Developmental / Q000139:chemically induced / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D004790:Enzyme Induction / Q000187:drug effects; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D011922:Rats, Mutant Strains
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls; D001205:Ascorbic Acid