title: [Expression of the gene for transforming growth factor type alpha, transferred into A431 cells by a recombinant retroviral vector].
abstract: Cells of human epidermoid carcinoma A431 were used for obtaining of the cell line constantly expressing TGF-alpha. Recombinant virions were obtained by introducing the proviral DNA into PA317 cells by means of electrotransfection. A protein with the EGF-competing activity was found in a conditioned media of the chosen clone A431/1522-4. The concentration of this protein was several times higher than in a conditioned media of wild type A431 cells. By means of electrophoresis, isoelectric focusing, and immunochemical analysis it was shown that the protein is TGF-alpha. Similarly to EGF, the extracted TGF-alpha entirely displaced 125I-EGF specifically bound to receptor. TGF-alpha produced by the A431/1522 cells also stimulated autophosphorylation of the EGF receptor.
authors.forename: P V
authors.initials: PV
authors.lastname: Pogrebnoĭ
authors.forename: S M
authors.initials: SM
authors.lastname: Serov
authors.forename: V A
authors.initials: VA
authors.lastname: Klenchin
authors.forename: A V
authors.initials: AV
authors.lastname: Luk'ianov
authors.forename: L V
authors.initials: LV
authors.lastname: Garmanchuk
authors.forename: D V
authors.initials: DV
authors.lastname: Tarnavskiĭ
authors.forename: N V
authors.initials: NV
authors.lastname: Markeeva
authors.forename: A I
authors.initials: AI
authors.lastname: Bykorez
mesh_terms: D001345:Autoradiography; D015139:Blotting, Southern; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing; D012190:Retroviridae / Q000235:genetics*; D016211:Transforming Growth Factor alpha / Q000032:analysis / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D007457:Iodine Radioisotopes; D016211:Transforming Growth Factor alpha
vernacular_title: Ekspressiia gena transformiruiushchego faktora rosta tipa alpha, perenesennogo rekombinantnym retrovirusnym vektorom v kletki A431.