[Airborne Japanese red cedar allergens studied by the immunoblotting technique--comparison with pollen counts obtained by Durham sampling and effect of weather].
We collected airborne particles with Burkard's sampling tape in Toyama from March to July. According to the immunoblotting technique (Takahashi et al, 1990), the airborne pollen allergens were reacted with anti-Cry j I polyclonal antibody and were stained as blue spots. The areas of these spots were measured with a color image analyzer (CIA) and a densitometer. There were significant correlations between the airborne Cry j I allergen spots (CIA and densitometer) and the pollen counts obtained with a Durham sampler (r = 0.624, 0.555, p < 0.001). The airborne Cry j I allergens however, tended to maintain a higher level than the pollen counts (Durham) in May. We theorized that this discrepancy is caused by cross reactivity with other pollen or crushed cedar pollen particles have allergenicity. There was a negative correlation between the Cry j I spots and rainfall. The pollen counts had a negative correlation with rainfall, and a positive correlation with average wind speed. These results suggest that this method is useful in evaluating fluctuations in airborne Cry j I allergen.
Takano
M
M
Department of Pediatrics, Faculty of Medicine, Toyama Medical & Pharmaceutical University.
Murakami
G
G
Matsuno
M
M
Onoue
Y
Y
Takayanagi
M
M
Kayahara
M
M
Adachi
Y
Y
Adachi
Y
Y
Okada
T
T
D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant; D015151:Immunoblotting; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D014197:Trees; D014887:Weather
D000485:Allergens; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; D010940:Plant Proteins