Effects of veratridine and cocaine on the kinetics of synaptosomal dopamine release.
Following preloading of striatal synaptosomes with 3H-dopamine (DA), the kinetics of release have been followed for a 60-min incubation period. DA appears to be totally releasable under both depolarizing (veratridine) and nondepolarizing conditions. Cocaine has no significant effect on release under either condition. Release is consistent with a model consisting of two parallel, linear compartments (when plotted as a log function). It is proposed that the slower compartment might represent the operation of the DA transporter, while the faster compartment might represent vesicular release.
Wheeler
D D
DD
Department of Physiology, Medical University of South Carolina, Charleston 29425.
Chapman
B M
BM
Ondo
J G
JG
D000818:Animals; D003042:Cocaine / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013574:Synaptosomes / Q000187:drug effects*; D014701:Veratridine / Q000494:pharmacology*
D014701:Veratridine; D003042:Cocaine; D004298:Dopamine