title: Demethylation of somatic and testis-specific histone H2A and H2B genes in F9 embryonal carcinoma cells.
abstract: In contrast to many other genes containing a CpG island, the testis-specific H2B (TH2B) histone gene exhibits tissue-specific methylation patterns in correlation with gene activity. Characterization of the methylation patterns within a 20-kb segment containing the TH2A and TH2B genes in comparison with that in a somatic histone cluster revealed that: (i) the germ cell-specific unmethylated domain of the TH2A and TH2B genes is defined as a small region surrounding the CpG islands of the TH2A and TH2B genes and (ii) somatic histone genes are unmethylated in both liver and germ cells, like other genes containing CpG islands, whereas flanking sequences are methylated. Transfection of in vitro-methylated TH2B, somatic H2B, and mouse metallothionein I constructs into F9 embryonal carcinoma cells revealed that the CpG islands of the TH2A and TH2B genes were demethylated like those of the somatic H2A and H2B genes and the metallothionein I gene. The demethylation of those CpG islands became significantly inefficient at a high number of integrated copies and a high density of methylated CpG dinucleotides. In contrast, three sites in the somatic histone cluster, of which two sites are located in the long terminal repeat of an endogenous retrovirus-like sequence, were efficiently demethylated even at a high copy number and a high density of methylated CpG dinucleotides. These results suggest two possible mechanisms for demethylation in F9 cells and methylation of CpG islands of the TH2A and TH2B genes at the postblastula stage during embryogenesis.
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260.
authors.forename: Y C
authors.initials: YC
authors.lastname: Choi
authors.forename: C B
authors.initials: CB
authors.lastname: Chae
mesh_terms: D000818:Animals; D001483:Base Sequence; D005796:Genes; D006657:Histones / Q000235:genetics*; D066298:In Vitro Techniques; D008297:Male; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013724:Teratoma / Q000235:genetics*; D013737:Testis / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D006657:Histones