title: Irradiation effects on wound contraction using a connective tissue model.
abstract: To analyze irradiation effects on wound contraction, fibroblasts harvested from human oral mucosa were irradiated (single 2-, 4-, 6-, 8-, and 10-Gy exposures of x-rays). The irradiated fibroblasts were examined for growth ability, and they were cultured three-dimensionally in hydrated collagen gels. The irradiation inhibited growth of fibroblasts and contraction of collagen gels in a dose-dependent manner. In addition, inhibition depended on the duration after exposure. The organization of actin filaments of fibroblasts in this model was examined with fluorescent dye-conjugated phalloidin. Many elongated cell projections were formed in cells of the control group, but the cell projections were almost disrupted in the irradiated cells. X-ray irradiation is thus shown to inhibit wound contraction by affecting the organization of actin filaments in fibroblasts.
authors.affiliation: Department of Oral Surgery, Nagoya University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yanase
authors.forename: M
authors.initials: M
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Kaneda
authors.forename: S
authors.initials: S
authors.lastname: Torii
authors.forename: M
authors.initials: M
authors.lastname: Matsuyama
mesh_terms: D000199:Actins / Q000528:radiation effects*; D002478:Cells, Cultured; D003094:Collagen / Q000528:radiation effects; D003599:Cytoskeleton / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D009061:Mouth Mucosa / Q000166:cytology*; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D000199:Actins; D003094:Collagen