[Determination of IgG toxoplasma antibodies using ELISA with uniform serum dilutions].
The ELISA reaction with uniform serum dilution (1:400) for assessment of toxoplasmatic antibodies class IgG agreed in 100% when compared with commercial kits (VIRELISA and GULL Lab.). Both these tests disagreed with the standard complement fixation reaction in 7.5% of the specimens. This qualitative disagreement was confirmed also in a group of 400 sera where 7.14% of CFR negative specimens reacted positively in the described ELISA reaction. Quantitative correlation of results of CFR and ELISA IgG displays the usual scatter of values. Therefore individual CFR titres cannot be matched unequivocally with titres of optic density and thus it is not possible to assess empirically the stage of infection. To assess the stage of infection it is therefore necessary to make a supplementary assessment of IgM antibodies. In cases detected in time the decision can be based on a rise of IgG values in paired serum specimens examined in one reaction. Dynamics of formation of IgG antibodies and their chronological sequence with IgM antibodies displayed a typical course in patients with clinical toxoplasmosis. The described reaction is the basis of commercially manufactured kits ELISA IgG Toxo Micro II.
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Národní referencní laborator pro toxoplazmózu a oportunnf parazitární nákazy.
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D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D014122:Toxoplasma / Q000276:immunology*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G
Stanovení toxoplazmových IgG protilátek metodou ELISA s jednotným redĕním sér.