title: Stimulation of development of in vitro-matured and in vitro-fertilized bovine embryos by platelets.
abstract: In vitro-fertilized bovine embryos were incubated in Menezo's B2 medium (MB2) supplemented with 2 mg/mL of BSA. In Exp. 1, eight-cell stage embryos were allotted to one of the following groups: control medium (MB2), MB2 with 20 ng/mL of platelet-activating factor (PAF), 1 x 10(7) bovine blood platelets (Platelets), oviductal cells (BOEC), BOEC and 20 ng/mL of PAF (BOEC+PAF), or BOEC and 1 x 10(7) platelets (BOEC+Platelets). In Exp. 2, eight-cell embryos were allotted to one of the following groups: control medium (MB2), MB2 with 1 x 10(7) platelets (Platelets), 1 x 10(7) platelets and 10 micrograms/mL of platelet-derived growth factor antibody (Platelets+anti-PDGF), 1 x 10(7) platelets and 1 microgram/mL of indomethacin (Platelets+Indomethacin), or 1 x 10(7) platelets and 3 micrograms/mL of mianserin (Platelets+Mianserin). Embryos were incubated at 39 degrees C in 5% CO2 in groups of five until 8 d after in vitro fertilization (IVF). In Exp. 1, Platelets stimulated embryo development to the morula, blastocyst, and expanded blastocyst stages. Embryo development was greatest in the BOEC+Platelets group on d 7 and 8 after IVF. Only embryos incubated in the BOEC+Platelets treatment group reached the hatched blastocyst stage on d 8. In Exp. 2, embryos incubated in the Platelets treatment group had the greatest (P < .05) proportion develop beyond the eight-cell stage. Embryos incubated in the Platelets + anti-PDGF group had less (P < .05) development beyond the eight-cell stage and to the morula stage. These results indicate that the stimulatory effects of PDGF on bovine embryo development may be derived from both the oviductal epithelium and platelets.
authors.affiliation: Department of Animal Science, Louisiana State University Agricultural Center, Baton Rouge 70803.
authors.forename: J K
authors.initials: JK
authors.lastname: Thibodeaux
authors.forename: R P
authors.initials: RP
authors.lastname: Del Vecchio
authors.forename: J R
authors.initials: JR
authors.lastname: Broussard
authors.forename: J F
authors.initials: JF
authors.lastname: Dickey
authors.forename: W
authors.initials: W
authors.lastname: Hansel
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D002417:Cattle / Q000196:embryology*; D002478:Cells, Cultured; D003470:Culture Media; D005314:Embryonic and Fetal Development; D005187:Fallopian Tubes / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D007213:Indomethacin / Q000494:pharmacology; D008803:Mianserin / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000096:biosynthesis / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000502:physiology; D011453:Prostaglandins / Q000502:physiology; D012701:Serotonin / Q000502:physiology
chemical_list: D003470:Culture Media; D010972:Platelet Activating Factor; D010982:Platelet-Derived Growth Factor; D011453:Prostaglandins; D008803:Mianserin; D012701:Serotonin; D007213:Indomethacin