Enhancement of immunoassay sensitivity by molecular modification of competitors.
The potential of weak competitors to enhance the sensitivity of competitive immunoassays is described. Several triazine derivatives have been analyzed for their use as competitors. Their binding properties were determined using an enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to triazines. Selected derivatives were immobilized onto the surface of a fibre optic sensor and atrazine was determined in a competitive manner using a fluorescein-labelled antibody. Using the weak binding competitor 11-(4-ethylamino-6-methylthio-s-triazine-2-yl)undecanoic acid (TE11S) the detection limit for atrazine could be lowered 100-fold in comparison to 2-aminohexylamino-4-ethylamino-6-isopropylamino-s-triazine (AHA), the previously used competitor.
Jockers
R
R
GBF-Gesellschaft für Biotechnologische Forschung mbH, Project Group Biosensors, Braunschweig, Germany.
Bier
F F
FF
Schmid
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RD
D000911:Antibodies, Monoclonal / Q000378:metabolism; D001280:Atrazine / Q000032:analysis* / Q000737:chemistry; D001667:Binding, Competitive; D002138:Calibration; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005336:Fiber Optic Technology; D006241:Haptens; D006540:Herbicides / Q000737:chemistry; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity; D014227:Triazines / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D006241:Haptens; D006540:Herbicides; D014227:Triazines; D001280:Atrazine; C010347:terbutryne