title: Phenotypic and molecular characterization of human monoclonal TCR gamma/delta T-cell lines from jejunum and colon of healthy individuals.
abstract: We have performed a phenotypic and molecular analysis of monoclonal TCR gamma/delta T-cell lines derived from jejunal and colonic biopsies of healthy individuals. Flow cytometric analysis employing a panel of 24 monoclonal antibodies (MoAbs) demonstrated that intestinal TCR gamma/delta intraepithelial lymphocytes (IEL) constitute a phenotypically heterogeneous population. Nucleotide sequence analysis of expressed TCR delta variable (V) regions revealed the dominant utilization of the V delta 2 and D delta 3 gene segments and frequent rearrangement of J delta 3. IEL V delta regions displayed extensive junctional diversity as a result of N and P insertion and the utilization of D delta 3 in all three reading frames. The results demonstrate that intestinal TCR gamma/delta T cells from healthy individuals constitute a phenotypically heterogeneous population expressing V delta regions that differ from their systemic counterparts.
authors.affiliation: Department of Gastroenterology, University Hospital Utrecht, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Robijn
authors.forename: H
authors.initials: H
authors.lastname: Bloemendal
authors.forename: S
authors.initials: S
authors.lastname: Jainandunsing
authors.forename: L J
authors.initials: LJ
authors.lastname: Wiegman
authors.forename: G P
authors.initials: GP
authors.lastname: VanBerge-Henegouwen
authors.forename: T
authors.initials: T
authors.lastname: Logtenberg
authors.forename: J C
authors.initials: JC
authors.lastname: Koningsberger
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002460:Cell Line; D003106:Colon / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D016130:Immunophenotyping; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology; D007583:Jejunum / Q000166:cytology / Q000276:immunology*; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016692:Receptors, Antigen, T-Cell, gamma-delta