Antipromoting effects of 5-lipoxygenase inhibitors, 3-nitro-2,4,6- trihydroxybenzamide derivatives, on TPA-promoted transformation in BALB 3T3 cells.
In order to discover the role in the promotion process of 5-lipoxygenase (5-LO), a key enzyme in the arachidonate cascade, the antipromoting effects of the 5-LO inhibiting 3-nitro-2,4,6-trihydroxybenzamide (NTB) derivatives were studied in a two-stage transformation assay system using BALB 3T3 cells. All compounds inhibited TPA-promoted transformation in a dose-dependent manner. Most of them achieved a 70-80% inhibition. Good correlations were observed between the inhibition of TPA-promoted transformation and that of 5-LO. These results indicate that 5-LO plays an important role in the promotion stage of the transformation of BALB 3T3 cells.
Semba
M
M
Quality Assurance Unit, Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
Honda
I
I
Shibagaki
M
M
Nishi
Y
Y
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D002471:Cell Transformation, Neoplastic; D004305:Dose-Response Relationship, Drug; D006877:Hydroxamic Acids / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors; D008748:Methylcholanthrene; D051379:Mice; D013755:Tetradecanoylphorbol Acetate
D006877:Hydroxamic Acids; D016859:Lipoxygenase Inhibitors; D008748:Methylcholanthrene; D013755:Tetradecanoylphorbol Acetate