Purification of chloroplast elongation factor Tu and cDNA analysis in tobacco: the existence of two chloroplast elongation factor Tu species.
We have purified a chloroplast elongation factor Tu (EF-Tu) from tobacco (Nicotiana tabacum) and determined its N-terminal amino acid sequence. Two distinct cDNAs encoding EF-Tu were isolated from a leaf cDNA library of N. sylvestris (the female progenitor of N. tabacum) using an oligonucleotide probe based on the EF-Tu protein sequence. The cDNA sequence and genomic Southern analyses revealed that tobacco chloroplast EF-Tu is encoded by two distinct genes in the nuclear genome of N. sylvestris. We designated the corresponding gene products EF-Tu A and B. The mature polypeptides of EF-Tu A and B are 408 amino acids long and share 95.3% amino acid identity. They show 75-78% amino acid identity with cyanobacterial and chloroplast-encoded EF-Tu species.
Murayama
Y
Y
Center for Gene Research, Nagoya University, Japan.
Matsubayashi
T
T
Sugita
M
M
Sugiura
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002736:Chloroplasts / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D008969:Molecular Sequence Data; D010444:Peptide Elongation Factor Tu / Q000235:genetics* / Q000302:isolation & purification; D010947:Plants, Toxic; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000648:ultrastructure
D004247:DNA; D010444:Peptide Elongation Factor Tu