[Immunoreactivity of synthetic peptides, corresponding to B-cellular epitopes of human type I T-lymphotrophic virus structural proteins].
The immunoreactivity of 25 synthetic peptides corresponding to amino acid fragments of the HTLV-I structural proteins p19 gag, gp46 and gp21 env were studied in enzyme linked immunosorbent assay using a serum panel of 70 reference positive specimens with anti-HTLV-I antibodies. The location of the synthetic peptides containing the B-cell epitopes of HTLV-I was established. Anti-HTLV-I antibodies effectively recognized these peptides. The significance of some amino acids for forming the HTLV-I antigenic determinants was estimated. The synthetic peptides with amino acid sequences 100-130 p19 gag and 176-201 gp46 env were found to have most immunoreactivity (90-99% recognition by sera of HTLV-I infected patients) and mimic the immunodominant B-cell epitopes of HTLV-I structural proteins.
Rasuli
A M
AM
Klepikov
N N
NN
Andreev
S M
SM
Sidorova
M V
MV
Vafina
M G
MG
Seniuta
N B
NB
Pavlish
O A
OA
Syrtsev
A V
AV
Gurtsevich
V E
VE
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015481:HTLV-I Antibodies / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015678:Viral Structural Proteins / Q000276:immunology*
D015481:HTLV-I Antibodies; D016056:Immunodominant Epitopes; D010455:Peptides; D015678:Viral Structural Proteins
Immunoreaktivnost' sinteticheskikh peptidov, sootvetstvuiushchikh B-kletochnym épitopam strukturnykh belkov T-limfotropnogo virusa cheloveka I tipa.