Immunological cross-reactivities between proteins secreted by the subcommissural organ, and plant lectins.
The glial subcommissural organ (SCO) discharges a glycoprotein-rich secretory product into the third ventricle to form Reissner's fibre (RF). The SCO proteins bear N-linked oligosaccharides, such as mediate many cell-cell and cell-matrix interactions. In such interactions the corresponding partner molecule recognising the sugar chain is often a sugar-specific protein (lectin). We present here evidence that the constituents of the SCO secretory product include proteins immunologically cross-reactive with certain plant lectins. Polyclonal antisera directed against Phaseolus vulgaris agglutinin-L (PHA-L) labelled the apically released RF-material of the rat SCO. Indirect ELISA studies shows in addition that anti-RF antisera bound to certain plant lectins (PHA-L, Con A). Dot spot assays demonstrated binding of anti-PHA-L to RF proteins. In Western blots of RF proteins anti-PHA-L, anti-RCA and anti-Con A bound to distinctive subsets of the RF protein fractions.
Debbage
P
P
Lehrstuhl III, Anatomische Anstalt, München, Germany.
Lehmann
W
W
Hanisch
U K
UK
Naumann
W W
WW
D000818:Animals; D015153:Blotting, Western; D003208:Concanavalin A / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology*; D007106:Immune Sera; D007150:Immunohistochemistry; D037102:Lectins / Q000276:immunology*; D010835:Phytohemagglutinins / Q000276:immunology; D037121:Plant Lectins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013351:Subcommissural Organ / Q000378:metabolism*; D014909:Wheat Germ Agglutinins / Q000276:immunology
D006023:Glycoproteins; D007106:Immune Sera; D037102:Lectins; D010835:Phytohemagglutinins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014909:Wheat Germ Agglutinins; C033619:lentil lectin; C100156:leukoagglutinins, plants; D003208:Concanavalin A