A dependable method for the synthesis of [14C]trehalose.
A new method for the preparation of [14C]trehalose was developed, based on the ability of yeast cells to accumulate trehalose under stress. The method is simple and reliable. It utilizes a yeast strain in which the gene that encodes for phosphoglucoisomerase has been deleted. Thus, exogenously supplied glucose is not metabolized, but is instead converted to trehalose. The [14C]-trehalose obtained is pure, it is hydrolyzed by trehalase, and it is not susceptible to the action of alpha-glucosidase. The yield of this method is in the order of 35% of the [14C]glucose supplied.
Stambuk
B U
BU
Departamento de Bioquímica, Universidade de S. Paulo, Brazil.
Crowe
J H
JH
Crowe
L M
LM
Panek
A D
AD
de Araujo
P S
PS
D002250:Carbon Radioisotopes; D005069:Evaluation Studies as Topic; D005632:Fructose / Q000378:metabolism; D017353:Gene Deletion; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D007700:Kinetics; D008722:Methods; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014199:Trehalose / Q000096:biosynthesis* / Q000302:isolation & purification
D002250:Carbon Radioisotopes; D005632:Fructose; D014199:Trehalose; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose