title: [Plant regeneration from hypocotyl protoplasts of Lycium barbarum L].
abstract: The hypocotyl protoplasts of Lycium barbarum L. CV. Ningji No. 1 were isolated in an enzyme mixture solution containing 1% cellulase and 1% pectinase. The protoplasts were cultured in KM 8 p liquid medium containing 0.3 mg/L 2,4-D and 0.3 mg/L BA, The first division of regenerated cells occurred 3 days after culture. The small cell clumps could be observed by naked eyes 20 days after culture. 40 days after culture, microcalli of 1-2 mm in size were transferred to MS solid differentiation medium containing 3% sugar, 0.1-0.4 mg/L BA and 0.05-0.1 mg/L NAA. About 30 days after culture on MS solid medium, the calli with shoots were transferred to 1/2 MS medium containing 0.1 mg/L BA and 0.2 mg/L IBA, from which 80% of them differentiated roots and regenerated whole plantlets.
authors.affiliation: Department of Biology, Wuhan University.
authors.forename: H Q
authors.initials: HQ
authors.lastname: Tian
authors.forename: Y H
authors.initials: YH
authors.lastname: Xiao
authors.forename: W F
authors.initials: WF
authors.lastname: Liu
mesh_terms: D046508:Culture Techniques; D010946:Plants, Medicinal / Q000254:growth & development*; D011523:Protoplasts