Human monoclonal anti-D-induced clearance of human D-positive red cells in a chimpanzee model.
Two human monoclonal anti-Rh0(D) antibodies, one IgG1, and one IgG3, were tested for their ability to clear human D-positive red blood cells (RBCs) from chimpanzee circulation. Human RBCs (phenotype A1, R1r) from 1 donor were radiolabelled with chromium 51 and injected into 4 chimpanzees. One day later the control animal received isotonic saline whereas 2 animals received 400 micrograms of purified human monoclonal anti-D, either IgG1 or IgG3. The remaining animal received both antibodies together (200 micrograms of IgG1 and 200 micrograms of IgG3). Both individual antibody-mediated clearance of human D-positive cells and synergy was not observed when both antibodies were used in combination. IgG1 was slightly more effective than IgG3. This animal model is a suitable alternative for conducting in vivo experiments in human beings, especially at the preclinical study phase of monoclonal anti-D antibodies.
Blancher
A
A
Laboratoire d'Anthropologie Physique du Collège de France, Paris.
Socha
W W
WW
Roubinet
F
F
Rowe
A W
AW
Broly
H
H
Byrne
P
P
Holuigue
M
M
Bouzidi
A
A
Huart
J J
JJ
Ruffié
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D002470:Cell Survival / Q000502:physiology; D004912:Erythrocytes / Q000276:immunology*; D006207:Half-Life; D006801:Humans; D007114:Immunization; D007275:Injections, Intravenous; D008954:Models, Biological; D002679:Pan troglodytes / Q000097:blood*; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen