Lectin-binding glycoconjugates in the tongues of the rat and guinea pig as revealed by lectin-gold-silver methods.
Lectin-gold-silver (LT-G-S) procedures using two lectins (RCA-I and Con A) were applied to appropriately prepared paraffin sections of tongues in the rat and guinea pig. In the lingual mucous membranes of the rat and guinea pig, positive RCA-I-G-S and Con A-G-S reactions were obtained in the intercellular spaces and the cytoplasm of epithelial cells of the basal and intermediate layers respectively. Likewise, the LT (RCA-I and Con A)-G-S techniques gave rise to varying intensities of positive reactions in the serous and mucous gland acini, nerve and muscle fiber bundles and connective tissue elements. The results obtained in the present study indicate that in the rat and guinea pig tongues the histologic structures mentioned contain varying amounts of beta-D-galactose and alpha-D-mannose or alpha-D-glucose residues of glycoconjugates, and that such histochemical properties of some of the lingual structures can be correlated with their possible histophysiological functions.
Ueda
T
T
Department of Anatomy, Nagoya City University Medical School, Japan.
Hokimoto
M
M
Shibata
N
N
Fujimori
O
O
Yamada
K
K
D000818:Animals; D005260:Female; D005632:Fructose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D006001:Glycoconjugates / Q000032:analysis*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D037102:Lectins; D008297:Male; D008358:Mannose / Q000032:analysis; D009092:Mucous Membrane / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014059:Tongue / Q000737:chemistry*
D006001:Glycoconjugates; D037102:Lectins; D005632:Fructose; D008358:Mannose; D005690:Galactose