Preparation of three troponin components from puffer skeletal muscle.
The Ca(2+)-regulatory proteins, i. e., troponin C, I and T, were prepared from puffer skeletal muscle. The SDS-gel electrophoretic study indicated that the molecular weights of troponin C, I and T were 18, 20 and 30k daltons, respectively. The Ca(2+)-sensitivity of the ATPase activity of the puffer myofibrils was abolished by the removal of troponin C by the CDTA-treatment and then restored by the reconstitution with puffer skeletal troponin C. The treatment with excess troponin T of either puffer or rabbit skeletal muscle at slightly acidic condition resulted in the removal of troponins C and I from the puffer myofibrils. The reconstituted puffer troponin C-I-T gave the Ca(2+)-sensitivity to the ATPase activity of puffer desensitized myofibrils in the presence tropomyosin.
Shiraishi
F
F
Department of Clinical Pharmacology, Faculty of Medicine, Kyushu University 60, Fukuoka.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D005399:Fishes; D006801:Humans; D008970:Molecular Weight; D009132:Muscles / Q000737:chemistry*; D009210:Myofibrils / Q000201:enzymology; D011817:Rabbits; D014336:Troponin / Q000302:isolation & purification* / Q000494:pharmacology; D019209:Troponin C; D019210:Troponin I; D020107:Troponin T
D014336:Troponin; D019209:Troponin C; D019210:Troponin I; D020107:Troponin T; D000251:Adenosine Triphosphatases; D002118:Calcium