Artificial mineralization in vitro--a model of tissue mineralization.
Calcium chloride and sodium hydrogen phosphate form precipitation zones in gelatin gel, gel supplemented with cartilaginous extract, articular cartilage slices, and in demineralized bone. The cartilaginous extract exerts a marked inhibitory effect on the crystal quantity and clustering. The pretreatment of cartilage slices with hyaluronidase increases the crystal frequency. The morphology of crystals formed in gelatin and in cartilage was similar but quite different from the precipitation pattern found in demineralized bone.
Smetana
K
K
Department of Anatomy, Charles University, First Faculty of Medicine, Praha.
Stol
M
M
Novák
M
M
D000818:Animals; D002113:Calcification, Physiologic; D002122:Calcium Chloride / Q000737:chemistry; D002358:Cartilage, Articular / Q000737:chemistry; D002417:Cattle; D002474:Cell-Free System; D011232:Chemical Precipitation; D005269:Femur / Q000737:chemistry; D005780:Gelatin; D066298:In Vitro Techniques; D008903:Minerals; D008954:Models, Biological; D010710:Phosphates / Q000737:chemistry; D013552:Swine
D008903:Minerals; D010710:Phosphates; D005780:Gelatin; D002122:Calcium Chloride; C018279:sodium phosphate