The effect of oestrogen ablation on the phospholipid metabolite content of primary and transplanted rat mammary tumours.
The concentration of phospholipid metabolites was determined in chemical extracts from two types of rat mammary tumours and compared with proliferation data (S-phase fraction). One of the tumours was an oestrogen-sensitive transplanted tumour. In this tumour the concentration of phosphocholine (PC) and glycerophosphorylcholine (GPC) correlated strongly with the S-phase fraction but not with the number of cells actively synthesizing DNA. Oestrogen ablation resulted in tumour regression. Regressing tumours contained less PC and more GPC than those actively growing. The other tumour was induced in rats by intravenous administration of N-methyl N-nitrosourea. Phosphoethanolamine (PE), PC and GPC levels were not associated with the S-phase fraction in this tumour. Oestrogen ablation resulted in tumour regression. There was no significant difference between the regressing and growing tumours in PE, PC or GPC content.
Smith
T A
TA
Department of Nuclear Medicine, Royal Marsden Hospital and Institute of Cancer Research, Sutton, Surrey, UK.
Baluch
S
S
Titley
J C
JC
Ormerod
M G
MG
Eccles
S
S
Tombs
A J
AJ
Leach
M O
MO
Griffiths
J R
JR
McCready
V R
VR
D000818:Animals; D002455:Cell Division; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005997:Glycerylphosphorylcholine / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008770:Methylnitrosourea; D009368:Neoplasm Transplantation; D010743:Phospholipids / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016196:S Phase
D004967:Estrogens; D010743:Phospholipids; D010767:Phosphorylcholine; D005997:Glycerylphosphorylcholine; D008770:Methylnitrosourea