Flexibility of DNA within transcriptionally active nucleosomes: analysis by circular dichroism measurements.
The conformational flexibility of DNA in transcriptionally active chromatin fractions has been estimated by circular dichroism spectroscopy analysis and was found to be restricted in the same fashion as in bulk chromatin. The observation is discussed in the context of different models of active chromatin organization.
Krajewski
W A
WA
Laboratory of Biochemistry, Russian Academy of Sciences, Moscow.
Panin
V M
VM
Krylov DYu
Razin
S V
SV
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism; D008961:Models, Structural; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013816:Thermodynamics; D014158:Transcription, Genetic
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA