Effects of cardioplegic reperfusion on ATP recovery of rod-shaped myocytes isolated from rat ventricles.
ATP content [ATP] and fraction of rod-shaped myocytes (Rrod) were measured in ventricular myocytes isolated from the rat heart. [ATP] (nmol (10(6) cells)-1) and Rrod (%) were 72.0 and 60.5, respectively, and significantly reduced to 18.8 and 41.8 by no-flow ischemia for 30 min. Reperfusion for 15 min with cardioplegic solutions restored [ATP] of rod-shaped myocytes to the control level.
Uchida
K
K
Department of Physiology, Yamagata University School of Medicine, Japan.
Tanaka
K
K
Doi
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002314:Cardioplegic Solutions; D066298:In Vitro Techniques; D007700:Kinetics; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002314:Cardioplegic Solutions; D000255:Adenosine Triphosphate