Semiquantitative determination of IL-1 alpha, TNF-alpha, PDGF-A, PDGF-B, and PDGF-receptor in term human placenta using polymerase chain reaction (PCR).
Eight placenta samples derived from normal term human placenta were examined for the expression levels of Il-1 alpha+, TNF-alpha+, PDGF-A+, PDGF-B+, and PDGF-receptor+ using a semiquantitative PCR protocol. The expressional level of beta-actin was proven to be constant, therefore beta-actin could serve to equilibrate sample size in our procedure. Differences were found in the mRNA levels of IL-1 alpha and TNF-alpha. The expression of PDGF-A, BDGF-B and PDGF-R seemed to be relatively constant in our group of samples. Further investigation and improved quantitative PCR protocols are needed to correlate clinical outcome of pregnancy with cytokine expression at the placental level. +Il-1 alpha (interleukin-1 alpha) +TNF-alpha (tumor necrosis factor-alpha) +PDGF-A (platelet derived growth factor A) +PDGF-B (platelet derived growth factor B) +PDGF-Rezeptor (platelet derived growth factor-rezeptor).
Heinig
J
J
Biochemical Institute of the University of Rostock.
Wilhelm
S
S
Bittorf
T
T
Müller
H
H
Brock
J
J
Briese
V
V
D000199:Actins / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D007375:Interleukin-1 / Q000235:genetics*; D010920:Placenta / Q000276:immunology*; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000235:genetics; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D000199:Actins; D007375:Interleukin-1; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C075278:platelet-derived growth factor A; D017479:Receptors, Platelet-Derived Growth Factor