High efficiency transformation of Kluyveromyces marxianus by a replicative plasmid.
Kluyveromyces marxianus can be transformed with an efficiency of 10(5) transformants/microgram of DNA by a replicative plasmid using electroporation. In order to obtain this efficiency, we isolated ura- mutants cells which can be complemented by the URA3 gene from Saccharomyces cerevisiae. The URA3 gene and KARS2, a replicative origin from Kluyveromyces lactis which functions in K. marxianus, were ligated together in a plasmid which can be used as a vector to transform this strain.
Iborra
F
F
Laboratoire de Biologie et Génetique Moléculaire, Institut de Génétique et Microbiologie Bat 400, Université Paris 11, Orsay, France.
D004261:DNA Replication; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics*; D008094:Lithium / Q000494:pharmacology; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics; D010957:Plasmids; D014170:Transformation, Genetic
D008094:Lithium; D009964:Orotidine-5'-Phosphate Decarboxylase