Comparison of five tests for the serologic diagnosis of myiasis by Gasterophilus spp. larvae (Diptera: Gasterophilidae) in horses and donkeys: a preliminary study.
Sera from 41 horses and 159 donkeys, from twelve States of México, were tested to ascertain anti-Gasterophilus circulating antibodies by double immunodiffusion (DD), counterimmunoelectrophoresis (CIE), indirect haemagglutination (IH), thin layer immunoassay (TIA) and diffusion-in-gel ELISA (DIG-ELISA) methods using crude somatic antigen from third instar larvae of G. intestinalis (DeGeer). At necropsy, 33/41 horses and 24/159 donkeys were found to be parasitized by G. intestinalis and/or G. nasalis (L.). Gasterophilus intestinalis was the species most commonly found in the equines. Analysis of the sera from the infected animals by DD showed positive results of 21.2% in horses and of 8% in donkeys. Screening the sera with CIE gave sensitivities of 69.7% in horses and of 32% in donkeys. Examination of the sera by IH showed positive results of 87.9% and of 48% in horses and donkeys, respectively. Testing the sera with TIA gave sensitivities of 93.9% in horses and of 96% in donkeys. Analysis of horses' sera by DIG-ELISA showed a sensitivity of 93.9%.
Escartin-Peña
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Centro Agropecuario, Posta Zootécnica, Universidad Autónoma de Aguascalientes, Morelos, México.
Bautista-Garfias
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CR
D000818:Animals; D000906:Antibodies / Q000097:blood; D004175:Diptera / Q000276:immunology; D005260:Female; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D008297:Male; D009198:Myiasis / Q000175:diagnosis / Q000662:veterinary*; D010527:Perissodactyla / Q000276:immunology / Q000469:parasitology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
D000906:Antibodies