The production of interleukin-1 receptor antagonist by human bronchogenic carcinoma.
Bronchogenic carcinoma displays an aggressive clinical course that may reflect a capacity to evade host defenses. We postulated that tumors may elaborate interleukin-1 receptor antagonist protein (IRAP) to escape host interleukin-1-dependent responses. Homogenates of human bronchogenic lung tumors demonstrated significant increases of IRAP compared with normal lung tissue controls (n = 48). There was no significant difference in interleukin-1 beta levels between tumor and normal lung tissue. Immunohistochemical staining localized IRAP to tumor cells. Semiquantitative pathological analysis demonstrated a modest inflammatory cell infiltrate with qualitative differences between tumors of different histology. Western blot analysis of tumor homogenates demonstrated several molecular weight forms of IRAP. Finally, antigenic IRAP was detected in supernatants of the human bronchogenic carcinoma cell line (A549) maintained in vitro. These findings illustrate the capacity of bronchogenic tumors to produce and secrete IRAP that may be important in tumor evasion of host defenses.
Smith
D R
DR
Department of Medicine, University of Michigan Medical School, Ann Arbor 48109-0360.
Kunkel
S L
SL
Standiford
T J
TJ
Chensue
S W
SW
Rolfe
M W
MW
Orringer
M B
MB
Whyte
R I
RI
Burdick
M D
MD
Danforth
J M
JM
Gilbert
A R
AR
D000230:Adenocarcinoma / Q000276:immunology; D015153:Blotting, Western; D002283:Carcinoma, Bronchogenic / Q000276:immunology* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D053590:Interleukin 1 Receptor Antagonist Protein; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D012795:Sialoglycoproteins / Q000032:analysis* / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D012795:Sialoglycoproteins