Cellulase production on high levels of cellulose and corn steep liquor.
Several enhanced cellulase mutants of Trichoderma reesei have been used for cellulase production using corn steep liquor and microcrystalline cellulose as substrates. Under the optimum fermentation conditions on 6% cellulose and 3.6% corn steep liquor Trichoderma reesei Rut C 30 produced high levels of cellulase enzymes 6MC-ase 54 IU/ml, filter paper activity 3.2 IU/ml after 10 days incubation at 28-30 degrees C.
Farid
M A
MA
Microbiology and Enzyme Chemistry Research Unit, National Research Centre, Dokki, Cairo, Egypt.
el-Shahed
K Y
KY
D000645:Ammonium Sulfate; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics; D003313:Zea mays
D003470:Culture Media; D002482:Cellulose; D002480:Cellulase; D000645:Ammonium Sulfate