Human placental cholesterol side-chain cleavage: enzymatic synthesis of (22R)-20 alpha,22-dihydroxycholesterol.
(22R)-20 alpha,22-Dihydroxycholesterol is the second intermediate in the conversion of cholesterol to pregnenolone by cytochrome P450scc in steroidogenic tissues. We report a rapid method for the enzymatic synthesis of (22R)-20 alpha,22-dihydroxycholesterol from (22R)-22-hydroxycholesterol using mitochondria from the human placenta.
Tuckey
R C
RC
Department of Biochemistry, University of Western Australia, Nedlands.
Cameron
K J
KJ
D002784:Cholesterol / Q000378:metabolism; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006888:Hydroxycholesterols / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D010920:Placenta / Q000201:enzymology* / Q000648:ultrastructure; D011247:Pregnancy; D011284:Pregnenolone / Q000378:metabolism
D006888:Hydroxycholesterols; C003563:20,22-dihydroxycholesterol; D011284:Pregnenolone; D002784:Cholesterol; D002786:Cholesterol Side-Chain Cleavage Enzyme