title: Experimental hepatitis E: pathogenesis in cynomolgus macaques (Macaca fascicularis).
abstract: The pathogenesis of experimental hepatitis E has not been thoroughly investigated. The purpose of this study was to more accurately document the events in this disease. Cynomolgus macaques were inoculated intravenously with bile or feces containing hepatitis E virus (HEV). Serum, bile, and liver specimens were evaluated with light microscopy, immune electron microscopy, immunofluorescence microscopy, EIA, and polymerase chain reaction. In the third week, there were histopathologic changes and HEV antigen (HEVAg) in liver, HEV in bile, and alanine aminotransferase (ALT) elevations. Widespread pathologic changes were detected during the fourth week and antibody to HEV (anti-HEV) and peak ALT values in the fifth or sixth week. By the sixth week, HEVAg had disappeared but pathologic changes persisted. This study supports the concept that experimental hepatitis E has an initial phase in which hepatic HEV replication is accompanied by the onset of hepatitis and a later phase in which the appearance of anti-HEV is accompanied by progression of the hepatitis.
authors.affiliation: Dept. of Virus Diseases, WRAIR, Washington DC, 20307-5100.
authors.forename: C F
authors.initials: CF
authors.lastname: Longer
authors.forename: S L
authors.initials: SL
authors.lastname: Denny
authors.forename: J D
authors.initials: JD
authors.lastname: Caudill
authors.forename: T A
authors.initials: TA
authors.lastname: Miele
authors.forename: L V
authors.initials: LV
authors.lastname: Asher
authors.forename: K S
authors.initials: KS
authors.lastname: Myint
authors.forename: C C
authors.initials: CC
authors.lastname: Huang
authors.forename: W F
authors.initials: WF
authors.lastname: Engler
authors.forename: J W
authors.initials: JW
authors.lastname: LeDuc
authors.forename: L N
authors.initials: LN
authors.lastname: Binn
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D001646:Bile / Q000382:microbiology; D005260:Female; D006508:Hepatitis Antibodies / Q000097:blood; D016751:Hepatitis E / Q000209:etiology* / Q000662:veterinary*; D016752:Hepatitis E virus / Q000302:isolation & purification; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008252:Macaca fascicularis; D008297:Male; D008992:Monkey Diseases / Q000209:etiology*; D013997:Time Factors
chemical_list: D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D000410:Alanine Transaminase