Detection of iron binding proteins by a blotting technique.
We describe a simple method for detecting iron binding proteins based on binding of 59Fe to protein blots from SDS gels followed by autoradiography. This binding is specific and is not affected by other metal ions. The method has allowed identification of new iron binding proteins in intestinal mucosa and in Hela cells.
Chen
Y
Y
Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111.
Drysdale
J
J
D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D006367:HeLa Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats; D014018:Tissue Distribution; D033863:Transferrin-Binding Proteins
D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins; D003101:Collodion; D007501:Iron