title: Enrichment of estradiol receptor from calf uterus.
abstract: A three-step procedure to enrich estradiol receptor protein from calf uterus nearly 30,000-fold has been described. Over-all yield is 12%. Control of the single steps has been performed by sodium dodecyl sulfate-electrophoresis, sucrose density gradient centrifugation, gel filtration and determination of receptor quantity. Immunological properties of the preparation obtained have not been controlled.
authors.affiliation: Central Institute for Cancer Research, Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Dietrich
authors.forename: H
authors.initials: H
authors.lastname: Wand
authors.forename: D
authors.initials: D
authors.lastname: Helbing
authors.forename: M
authors.initials: M
authors.lastname: Görlich
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D011959:Receptors, Estradiol / Q000032:analysis / Q000302:isolation & purification*; D014599:Uterus / Q000737:chemistry*
chemical_list: D011959:Receptors, Estradiol; D000645:Ammonium Sulfate