Voltage gradient electrophoresis of nucleic acids on agarose gels.
A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about 30 kb) in the same electrophoresis agarose gel. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, submerged in the electrophoretic running buffer. Further applications of our system are also described.
Barbieri
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R
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
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D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D008970:Molecular Weight; D010957:Plasmids / Q000302:isolation & purification
D004247:DNA