title: Intracellular calcium oscillations induced in a T-cell line by a weak 50 Hz magnetic field.
abstract: Applied weak magnetic fields have been shown to affect cellular activity on several levels, but the mechanisms involved remain elusive. We have decided to study an early signal transduction event in the human T cell line Jurkat; oscillations of free [Ca2+]i, of the type seen by crosslinking the CD3 complex. Cells were exposed to a 50 Hz, 0.1 mT, sinusoidal magnetic field while intracellular free calcium was measured in individual cells, using fura-2 as a probe. An acute response was observed with oscillatory increases in [Ca2+]i, which subsided when the field was turned off. The effect of the magnetic field on [Ca2+]i was comparable to that achieved by an anti-CD3 monoclonal antibody.
authors.affiliation: Department of Cell and Molecular Biology, Umeå University, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Lindström
authors.forename: P
authors.initials: P
authors.lastname: Lindström
authors.forename: A
authors.initials: A
authors.lastname: Berglund
authors.forename: K H
authors.initials: KH
authors.lastname: Mild
authors.forename: E
authors.initials: E
authors.lastname: Lundgren
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D017252:CD3 Complex / Q000276:immunology; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D005470:Fluorometry; D016257:Fura-2; D006801:Humans; D007942:Leukemia, Experimental; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000473:pathology*; D008214:Lymphocytes / Q000378:metabolism / Q000502:physiology; D008280:Magnetics; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D002118:Calcium; D016257:Fura-2