Simultaneous genotyping of sex and kappa-casein of bovine in vitro fertilized embryos by the PCR technique.
Sex and the kappa-casein genotype of in vitro produced cattle demiembryos was identified by simultaneous PCR-genotyping using polymorphic sites in ZFY/ZFX and kappa-casein genes. We obtained PCR products of 530 bp (kappa-casein) and of 445/447 bp (sex). Sex and the two kappa-casein alleles could be identified after digestion of the PCR products with Pstl and simultaneous separation of the restriction fragments on one step, allowing a rapid and efficient analyses of embryos.
Schellander
K
K
Institute of Animal Breeding and Genetics, Veterinary University, Vienna, Austria.
Mayr
B
B
Ertl
K
K
Peli
J
J
D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D002417:Cattle / Q000196:embryology*; D004277:DNA, Single-Stranded / Q000737:chemistry; D005307:Fertilization in Vitro; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis
D002364:Caseins; D004277:DNA, Single-Stranded