A transformant of Aspergillus fumigatus deficient in the antigenic cytotoxin ASPFI.
The aspfI gene encoding a ribonucleotoxin, a putative virulence factor of Aspergillus fumigatus, was inactivated by gene disruption. Gene replacement through homologous recombination by the disrupted allele tagged by the hygromycin B resistance marker was performed by transformation of a pathogenic strain. One transformant with the disrupted aspfI gene failed to produce the ASPFI protein and was shown to be pathogenic for mice. We concluded that this ribotoxin is not a main factor in the colonization of the lung tissues by A. fumigatus.
Paris
S
S
Unite de Mycologie, Institut Pasteur, Paris, France.
Monod
M
M
Diaquin
M
M
Lamy
B
B
Arruda
L K
LK
Punt
P J
PJ
Latgé
J P
JP
D000485:Allergens; D000818:Animals; D052179:Antigens, Plant; D001228:Aspergillosis / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D003603:Cytotoxins / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005656:Fungal Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D051379:Mice; D010957:Plasmids; D015183:Restriction Mapping; D012260:Ribonucleases; D014170:Transformation, Genetic; D014774:Virulence / Q000235:genetics
C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D052179:Antigens, Plant; D003603:Cytotoxins; D004271:DNA, Fungal; D005656:Fungal Proteins; C036196:MITF protein, Aspergillus restrictus; D012260:Ribonucleases