A new pressure chamber to study the biosynthetic response of articular cartilage to mechanical loading.
A prototype chamber was used to apply a precise cyclic or static load on articular cartilage explants under sterile conditions. A variable pressure, pneumatic controller was constructed to power the chamber's air cylinder, capable of applying, with a porous load platen, loads of up to 10 MPa at cycles ranging from 0 to 10 Hz. Pig articular cartilage explants were maintained successfully in this chamber for 2 days under cyclic mechanical loading of 0.5 Hz, 0.5 MPa. Explants remained sterile, viable and metabolically active. Cartilage responded to this load with a decreased synthesis of fibronectin and a small but statistically significant elevation in proteoglycan content. Similar but less extensive effects on fibronectin synthesis were observed with the small static load (0.016 MPa) inherent in the design of the chamber.
Steinmeyer
J
J
Institut für Pharmakologie und Toxikologie der Universität, Bonn, Germany.
Torzilli
P A
PA
Burton-Wurster
N
N
Lust
G
G
D000818:Animals; D002358:Cartilage, Articular / Q000502:physiology*; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000096:biosynthesis*; D011509:Proteoglycans / Q000096:biosynthesis*; D013314:Stress, Mechanical; D013552:Swine
D003470:Culture Media; D005353:Fibronectins; D011509:Proteoglycans