Methods for the quantitation of bromosulfophthalein and its glutathione conjugate in biological fluids.
This paper describes a solvent-gradient HPLC method which was developed for the quantitation of bromosulfophthalein (BSP) in erythrocyte and albumin containing (blood) perfusate samples, and of BSP and its glutathione conjugate (BSP-GSH) in bile samples obtained from rat liver perfusion experiments. Phenolphthalein was used as an internal standard. The cysteinyl-, N-acetylcysteinyl-, and cysteinylglycinyl-BSP conjugates did not interfere with the HPLC assay and cysteine, N-acetylcysteine, [3H]GSH, and [3H]GSSG eluted within the first 6 min postinjection onto the HPLC system. A spectrophotometric method was also developed for the rapid quantitation of BSP in blood perfusate; tracer [14C]urea was utilized as the internal standard. Although the spectrophotometric method was less sensitive than the HPLC method, good correlation was found to exist between the methods.
Snel
C A
CA
Faculty of Pharmacy, University of Toronto, Canada.
Zhao
Y
Y
Mulder
G J
GJ
Pang
K S
KS
D000818:Animals; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005978:Glutathione / Q000032:analysis* / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods / Q000706:statistics & numerical data; D013448:Sulfobromophthalein / Q000032:analysis*
D013448:Sulfobromophthalein; D005978:Glutathione