title: Avian gut-associated immune system: implication in coccidial vaccine development.
abstract: Host responses to coccidia are complex and involve both humoral and cellular immune mechanisms. Convincing evidence exists for a major role of cell-mediated immunity in anticoccidial resistance. With an increasing need for the development of coccidial vaccine, understanding of the intestinal immune system is crucial. To provide increased insight into the immune mechanisms involving mucosal immune response to coccidia, cellular events involved in the maturation of intestinal immune system were studied. Postnatal development of various T lymphocyte subpopulations expressing CD3, CD8, CD4, and antigen-specific T cell receptor (TCR) heterodimers expressing gamma delta (TCR1) or alpha beta (TCR2) was investigated in chickens. Intraepithelial lymphocytes (IEL) expressing the CD8 antigen increased gradually following hatching and subsequently declined with age. The CD4+ cells represented a minor subpopulation among the IEL. The ratios of IEL T cells expressing gamma delta (TCR1) or alpha beta (TCR2) in the intraepithelium of chickens gradually increased after hatching. These results indicate maturational changes of intestinal immune system that should be considered in the development of vaccine against coccidia.
authors.affiliation: USDA, Agricultural Research Service, Beltsville Agricultural Research Center, Maryland 20705.
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens / Q000276:immunology*; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary*; D007111:Immunity, Cellular; D007413:Intestinal Mucosa / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal