title: Effect of homologous fibrin adhesive on callus formation and extracortical bone bridging around a porous-coated segmental endoprosthesis in dogs.
abstract: Modular, porous-coated, titanium segmental endoprostheses were implanted bilaterally in the femoral diaphysis of 7 adult mixed-breed dogs. Autogenous bone graft in particle form was placed around the implant and bone. In 1 limb, homologous fibrin adhesive was mixed with the graft in situ before soft tissue closure. The contralateral limb was grafted in identical manner, but without fibrin adhesive, and served as a control. Radiography was performed immediately after surgery and 1, 2, 3, 4, 6, 8, 10, and 12 weeks later to assess callus area and bone remodeling. At 12 weeks, dogs were euthanatized and bone/implant fixation strength was tested under torsion and compared with values for 6 in vitro controls. Histomorphometric and microradiographic analyses of transverse sections of the distal portion of the implanted femurs were performed. Radiographic callus area was significantly (P < 0.05) smaller in the femurs grafted with fibrin adhesive, compared with the contralateral control. New bone formation (21.4 +/- 1.8% vs 19.2 +/- 2.4%), unlabeled bone (64.8 +/- 3.0% vs 67.9 +/- 4.2%), porosity (13.9 +/- 0.7% vs 12.9 +/- 0.8%), and bone ingrowth into the porous coating (10.3 +/- 0.9% vs 10.0 +/- 1.2%) were not significantly different between fibrin- and nonfibrin-grafted implants, respectively. There were no significant differences in torsional strength of implant fixation between the fibrin- and nonfibrin-grafted femurs or between the in vivo implanted femurs and the in vitro controls.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Small Animal Clinical Sciences, University of Minnesota College of Veterinary Medicine, St Paul 55108.
authors.forename: R G
authors.initials: RG
authors.lastname: Roy
authors.forename: M D
authors.initials: MD
authors.lastname: Markel
authors.forename: A J
authors.initials: AJ
authors.lastname: Lipowitz
authors.forename: F
authors.initials: F
authors.lastname: Gottsauner-Wolf
authors.forename: H F
authors.initials: HF
authors.lastname: Taswell
authors.forename: E Y
authors.initials: EY
authors.lastname: Chao
mesh_terms: D000269:Adhesives / Q000494:pharmacology*; D000818:Animals; D002146:Bony Callus / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D004283:Dog Diseases; D004285:Dogs; D004867:Equipment Design; D005260:Female; D005264:Femoral Fractures / Q000601:surgery / Q000662:veterinary*; D005269:Femur / Q000000981:diagnostic imaging; D005337:Fibrin / Q000494:pharmacology*; D016268:Internal Fixators / Q000662:veterinary*; D011859:Radiography; D013997:Time Factors
chemical_list: D000269:Adhesives; D005337:Fibrin