title: Control analysis applied to single enzymes: can an isolated enzyme have a unique rate-limiting step?
abstract: Control analysis is used to analyse and quantify the concept of a rate-limiting step within an enzyme. The extent to which each rate constant within the enzyme limits the steady-state rate of the enzyme and the levels of enzyme intermediate species are quantified as flux and concentration control coefficients. These coefficients are additive and obey summation theorems. The control coefficients of triose phosphate isomerase, carbamate kinase and lactate dehydrogenase are calculated from literature values of the rate constants. It is shown that, contrary to previous assumption, these enzymes do not have a unique rate-limiting step, but rather flux control is shared by several rate constants and varies with substrate, product and effector concentrations, and with the direction of the reaction. Thus the general assumption that an enzyme will have a unique rate-limiting step is unjustified.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, U.K.
authors.forename: G C
authors.initials: GC
authors.lastname: Brown
authors.forename: C E
authors.initials: CE
authors.lastname: Cooper
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D013379:Substrate Specificity
chemical_list: D004798:Enzymes