Dp71, the nonmuscle product of the Duchenne muscular dystrophy gene is associated with the cell membrane.
The 70.8 kDa protein, Dp71, is the major Duchenne muscular dystrophy (DMD) gene product in many nonmuscle tissues including the brain. Dp71 shares most of the C-terminal and cysteine-rich domains with the dystrophins but lacks the entire large rod shaped domain of spectrin-like repeats, and the N-terminal actin-binding domain. The function of Dp71 is unknown. Using subcellular fractionation and immunostaining we show that Dp71 is associated with the plasma membrane. Dp71 is also associated with the plasma membrane in mdx myogenic cells transfected with a vector expressing Dp71.
Rapaport
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Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Greenberg
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Tal
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Yaffe
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Nudel
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D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000378:metabolism; D016189:Dystrophin / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000235:genetics / Q000378:metabolism*; D011506:Proteins; D013347:Subcellular Fractions; D014162:Transfection; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D016189:Dystrophin; D011506:Proteins