The complete nucleotide sequence of African horsesickness virus serotype 4 (vaccine strain) segment 4, which encodes the minor core protein VP4.
The complete sequence of RNA segment 4 of African horsesickness virus serotype 4 (AHSV-4) vaccine strain was determined from the full-length cDNA clone inserted into pBR322. The RNA is 1978 bp long (M(r) 1.27 x 10(6)) and contains an open reading frame encoding a protein of 642 amino acids (M(r) 75826) with a net charge of +10 at neutral pH. The 5' and 3' termini of AHSV-4 segment 4,5'GTTTAT... and ...CCTTAC3', were different from orbivirus characteristic terminal sequences, being 5'GTTAAA... and ...ACTTAC3'. A comparison of the sequence of AHSV-4 segment 4 with that of bluetongue virus (BTV) serotype 10 revealed 55.4% nucleotide similarity and 48.5% amino acid similarity. In addition, Northern blot hybridization showed that the full-length AHSV-4 segment 4 cDNA cross-hybridized well with the corresponding genes of serotype 1, 2, 3, 4 and 7 but slightly with serotype 5, 6 and 8 of attenuated AHSV.
Mizukoshi
N
N
Exotic Diseases Research Division, National Institute of Animal Health, Kodaira, Tokyo, Japan.
Sakamoto
K
K
Iwata
A
A
Tsuchiya
T
T
Ueda
S
S
Apiwatnakorn
B
B
Kamada
M
M
Fukusho
A
A
D000356:African Horse Sickness Virus / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004279:DNA, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D014765:Viral Vaccines / Q000235:genetics
D036022:Capsid Proteins; D004279:DNA, Viral; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus; D014765:Viral Vaccines