An antigen processing polymorphism revealed by HLA-B8-restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism.
In previous studies of antigen presentation through HLA-B27, we identified a healthy person whose lymphoblastoid cells do not present three B27-restricted viral epitopes to specific cytotoxic T lymphocytes (CTL), despite adequate cell surface expression of HLA-B2702 of normal sequence. Similar findings were observed in all members of his family sharing the HLA-A3-B2702 haplotype. The original donor, NW, carries HLA-B8 on his other class I haplotype, which his daughter, HW, has inherited. We now report a failure to present an HLA-B8-restricted epitope from influenza nucleoprotein following viral infection of NW cells, although exogenous added peptide is still presented normally. However, cells from HW, which do not carry the A3-B2702 haplotype, present the expected epitope after viral infection. Another B8-restricted epitope, from human immunodeficiency virus-gag, is presented equally well by both cell lines when infected with gag-vaccinia. This antigen processing phenotype does not correlate with any of the known human TAP-1 and TAP-2 polymorphisms.
Rowland-Jones
S L
SL
Molecular Immunology Group, John Radcliffe Hospital, Headington, Oxford, GB.
Powis
S H
SH
Sutton
J
J
Mockridge
I
I
Gotch
F M
FM
Murray
N
N
Hill
A B
AB
Rosenberg
W M
WM
Trowsdale
J
J
McMichael
A J
AJ
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D000938:Antigen-Presenting Cells / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics*; D005260:Female; D015795:HLA-B8 Antigen / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008297:Male; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000276:immunology; D011110:Polymorphism, Genetic; D016601:RNA-Binding Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D015795:HLA-B8 Antigen; D000949:Histocompatibility Antigens Class II; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D016601:RNA-Binding Proteins; C073119:TAP1 protein, human; D014758:Viral Core Proteins; C071692:TAP2 protein, human