In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco.
The promoter region of the Agrobacterium tumefaciens T-cyt gene was fused to a beta-glucuronidase (gusA) reporter gene and introduced into tobacco plants. Detection of gusA expression in transgenic F1 progeny revealed that the T-cyt promoter is active in many, if not all, cell types in leaves, stems and roots of fully developed plants. Developmental stage-dependent promoter activity was observed in seedlings. Analysis of 5'-deleted promoter fragments showed that sequences located between positions -185 and -139 with respect to the T-cyt translational start codon are essential for T-cyt promoter activity in transfected tobacco protoplasts as well as in transformed tobacco plants.
Neuteboom
S T
ST
Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Netherlands.
Hulleman
E
E
Schilperoort
R A
RA
Hoge
J H
JH
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D003001:Cloning, Molecular; D003583:Cytokinins / Q000235:genetics*; D004269:DNA, Bacterial; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011523:Protoplasts; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology*; D014162:Transfection
D003583:Cytokinins; D004269:DNA, Bacterial; C036483:T-DNA