Identification of the putative hexose-phosphate translocator of amyloplasts from cauliflower buds.
Starch synthesis in amyloplasts isolated from cauliflower buds is strongly inhibited by the addition of micromolar concentrations of 4,4'-di-isothiocyano-2,2'-stilbenedisulphonic acid (DIDS). Using [3H]DIDS it was possible to label specifically a 31.6 kDa membrane protein of the envelope fraction of isolated amyloplasts. The intensity of the radioactive label was decreased in the presence of glucose 6-phosphate or dihydroxyacetone phosphate, indicating that this protein might be the amyloplastic hexosephosphate translocator.
Batz
O
O
Fachbereich Biologie/Chemie, Universität Osnabrück, Federal Republic of Germany.
Scheibe
R
R
Neuhaus
H E
HE
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D001937:Brassica / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004099:Dihydroxyacetone Phosphate / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000494:pharmacology; D015388:Organelles / Q000378:metabolism*; D013213:Starch / Q000096:biosynthesis
D002352:Carrier Proteins; D005958:Glucosephosphates; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D019298:Glucose-6-Phosphate; D004099:Dihydroxyacetone Phosphate; D013213:Starch; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid