Pre-column derivatization and its optimum conditions for quantitative determination of urinary hydroxylysine glycosides by high-performance liquid chromatography.
A method has been developed for rapid analysis of the urinary hydroxylysine glycosides [glucosyl-galactosyl-hydroxylysine (GGH) and galactosyl-hydroxylysine (GH)] as collagen metabolites. The glycosides were fluorometrically derivatized with 5-dimethylaminonaphthalene-1-sulfonyl chloride, and then subjected to reversed-phase high-performance liquid chromatography using a gradient system. Suitable standard hydroxylysine glycosides which were not diastereoisomers were prepared from human urine. Urinary GGH and GH were analyzed within 1 h, including column washing. This method was applied to the urine samples of 68 healthy persons of different ages.
Yoshihara
K
K
Tokyo College of Pharmacy, Japan.
Mochidome
N
N
Shida
Y
Y
Hayakawa
Y
Y
Nagata
M
M
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000032:analysis / Q000138:chemical synthesis; D006027:Glycosides / Q000652:urine; D006801:Humans; D006901:Hydroxylysine / Q000652:urine*; D007202:Indicators and Reagents; D008875:Middle Aged; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D003619:Dansyl Compounds; D006027:Glycosides; D007202:Indicators and Reagents; D006901:Hydroxylysine