Measurement of ascorbic acid in human aqueous humour and plasma and bovine aqueous humour by high-performance liquid chromatography with electrochemical detection.
High-performance liquid chromatography (HPLC) on ODS silica with cetyltrimethylammonium bromide as an ion-pairing reagent and electrochemical detection (ECD) was used to determine ascorbic acid in bovine and human aqueous humour and human plasma. Hydroquinone was used as the internal standard. A calibration curve plotted with ascorbic acid concentrations in the range 0.5-5 micrograms ml-1 for peak height versus internal standard peak height had a correlation coefficient of 0.998. The RSD (precision) between analyses of the same diluted sample was 1.5% and the RSD (reproducibility) between analyses of separate aliquots of the same sample of aqueous humour was 1.6%.
Watson
D G
DG
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
Iqbal
Z
Z
Midgley
J M
JM
Pryce-Jones
H
H
Morrison
L
L
Dutton
G N
GN
Karditsas
S
S
Wilson
W
W
D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D001205:Ascorbic Acid / Q000032:analysis* / Q000097:blood; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D001205:Ascorbic Acid