A high-sensitivity electrochemiluminescence-based detection system for automated PCR product quantitation.
A high-sensitivity nonisotopic system has been developed for post-PCR product detection. The probe-based detection system exploits a chemiluminescent reaction that takes place on the electrode surface in an electrochemical cell. The detection system incorporates a biotin-streptavidin capture reaction onto a solid support that permits fast post-PCR product detection at the attomole level. The system precision is within 5% relative standard deviation over a linear dynamic range of greater than three orders of magnitude. In this paper, the principles and features of the electrochemiluminescent-based detection system, together with its application to PCR product quantitation, are described in detail.
DiCesare
J
J
Perkin-Elmer Corporation, Wilton, CT 06897.
Grossman
B
B
Katz
E
E
Picozza
E
E
Ragusa
R
R
Woudenberg
T
T
D001322:Autoanalysis / Q000592:standards / Q000706:statistics & numerical data; D001483:Base Sequence; D004563:Electrochemistry; D005914:Globins / Q000235:genetics; D006801:Humans; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D011786:Quality Control
D005914:Globins