title: Inhibitory effect of beta-glucans on zymosan-mediated hydrogen peroxide production by murine peritoneal macrophages in vitro.
abstract: Effects of the pretreatment of murine peritoneal macrophages with several polysaccharides on the production of H2O2 induced with unopsonized zymosan were examined. Pretreatment with most of (1-->3)-beta-D-glucans for 6 h at 37 degrees C inhibited the zymosan-mediated H2O2 production by macrophages. The phorbol myristate acetate (PMA)-mediated H2O2 production was not affected by the pretreatment. The pretreatment of macrophages with (1-->3)-beta-D-glucans decreased the ability to ingest unopsonized zymosan, but did not affect the ingestion of IgG-coated sheep red blood cells (IgG-SRBC). These results suggested that the pretreatment with (1-->3)-beta-D-glucans interfered with the interaction of macrophages to zymosan and that the occupation of the receptor for the (1-->3)-beta-D-glucans inhibited zymosan-mediated production of H2O2 by macrophages. Chemical modification by substitution with carboxymethyl groups or hydroxyethyl groups of a (1-->6)-branched (1-->3)-beta-D-glucan reduced the inhibitory effect of pretreatment on zymosan-mediated H2O2 production. The above results indicated the possibility that murine peritoneal macrophages possess certain receptors for beta-anomeric glucans, and one ligand specificity of the receptors is to restrict the intact (1-->3)-beta-D-glucosyl back bone.
authors.affiliation: Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Adachi
authors.forename: N
authors.initials: N
authors.lastname: Ohno
authors.forename: T
authors.initials: T
authors.lastname: Yadomae
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004912:Erythrocytes / Q000276:immunology; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology; D012756:Sheep / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology*
chemical_list: D005936:Glucans; D007074:Immunoglobulin G; D011134:Polysaccharides; D015054:Zymosan; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate