Cell-specific expression of Gq/11 protein and mRNA in rat seminiferous tubules.
As part of a study to elucidate the involvement of G proteins in signal transduction in testicular cells, we have examined the cellular localization of Gq/11 within the seminiferous tubules. The somatic cells (Sertoli cells, peritubular cells) contain high amounts of both Gq/11 alpha mRNA and immunoreactive protein. In contrast, very low levels of these G proteins and the corresponding mRNAs are present in the germ cells (pachytene spermatocytes, round spermatids). Thus, in the germ cells, receptor-regulated inositol phospholipid hydrolysis is not likely to be regulated via Gq/11, but rather through the Go protein, which has been previously shown to be abundant in rat germ cells. Since the somatic cells are nearly devoid of Go, the Gpp(NH)p-stimulated phospholipase C in these cells is probably regulated by Gq and/or G11.
Haugen
T B
TB
Institute of Medical Biochemistry, University of Oslo, Norway.
Paulssen
R H
RH
Hansson
V
V
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012671:Seminiferous Tubules / Q000166:cytology / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism
D012333:RNA, Messenger; D019204:GTP-Binding Proteins