title: [Inhibition of phospholipase A2 of peritoneal macrophages in rats by 1,2-di-O-hexadecyl-rac-glycero-3-phosphocholine].
abstract: The 1-O-stearoyl-2-O-[3H] arachidonyl-sn-glycero-3-phosphocholine, introduced in the culture medium, was taken up by the peritoneal macrophages activated by the ionophore A 23187. After intracellular phospholipase A2 activity, the [3H] arachidonic acid was found in cells and in extracellular fluids. It also reached the eicosanoid synthesis. When it was introduced in the culture medium with the tritiated phospholipid, the 1, 2 di-O-hexadecyl-rac-glycero-3-phosphocholine, which has a non hydrolysable alkylated structure in the 2 position of the glycerol, inhibited the intracellular phospholipase A2, then contributed to lower the eicosanoid synthesis.
authors.affiliation: Laboratoire de Pharmacologie et Pharmacocinétique, Nantes.
authors.forename: P
authors.initials: P
authors.lastname: Boucrot
authors.forename: E N
authors.initials: EN
authors.lastname: Khettab
authors.forename: J Y
authors.initials: JY
authors.lastname: Petit
authors.forename: L
authors.initials: L
authors.lastname: Welin
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D015777:Eicosanoids / Q000096:biosynthesis; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054467:Phospholipases A2; D010742:Phospholipid Ethers / Q000494:pharmacology*; D010743:Phospholipids / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D015777:Eicosanoids; D005230:Fatty Acids, Nonesterified; D010742:Phospholipid Ethers; D010743:Phospholipids; D000001:Calcimycin; D010741:Phospholipases A; D054467:Phospholipases A2
vernacular_title: Inhibition de la phospholipase A2 des macrophages péritonéaux de rat par la 1,2-di-O-hexadécyl-rac-glycéro-3-phosphocholine.