Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination.
Recombination walking is based on the genetic selection of specific human clones from a yeast artificial chromosome (YAC) library by homologous recombination. The desired clone is selected from a pooled (unordered) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walking represents an efficient approach to library screening and is well suited for chromosome-walking approaches to the isolation of genes associated with common diseases.
Miller
A M
AM
Transkaryotic Therapies, Inc., Cambridge, MA 02139.
Savinelli
E A
EA
Couture
S M
SM
Hannigan
G M
GM
Han
Z
Z
Selden
R F
RF
Treco
D A
DA
D000818:Animals; D002874:Chromosome Mapping; D016386:Chromosome Walking; D015825:Chromosomes, Fungal; D005800:Genes, Fungal; D015698:Genomic Library; D005838:Genotype; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012641:Selection, Genetic