Reevaluation of the linkage between acute hemorrhagic shock and bacterial translocation in the rat.
The present study was undertaken to determine the conditions under which acute periods of hemorrhagic shock induce bacterial translocation. Rats (at least six per group) were anesthetized intraperitoneally with the barbiturate, pentobarbital (50 or 65 mg/kg), or the inhalation anesthetic methoxyflurane. Following anesthesia, the femoral artery was catheterized, from which blood was withdrawn to maintain a mean arterial blood pressure of 30 mmHg for 30, 60, or 90 min, followed by reinfusion of shed blood. Instrumented, but nonshocked animals served as controls. Rats were sacrificed at 0, 2, or 24 hr postshock, and quantitative bacterial cultures of the mesenteric lymph node complex (MLN), liver, and spleen were made. Within groups, the effects of heparinization were also determined. In pentobarbital-treated animals, regardless of the extent of heparinization, consistent translocation to both MLN and distant organs occurred when shock was prolonged for 90 min, and assessment of translocation was made 24 hr after reinfusion of shed blood. Furthermore, a mortality rate of approximately 30% was found in rats subjected to this protocol. The magnitude of translocation was less consistent, and did not differ from that in sham shock controls, under other conditions of shock and evaluation. In rats anesthetized with methoxyflurane, no mortality occurred, and no statistical significance between the incidence or degree of translocation in shocked animals vs. sham shock controls could be demonstrated, regardless of the shock protocol. In additional studies, effects of these anesthetics on intestinal morphology and superior mesenteric arterial (SMA) flow in the context of hemorrhagic shock were assessed.(ABSTRACT TRUNCATED AT 250 WORDS)
LaRocco
M T
MT
Department of Surgery, University of Texas Medical School, Houston 77030.
Rodriguez
L F
LF
Chen
C Y
CY
Smith
G S
GS
Russell
D H
DH
Myers
S I
SI
Cocanour
C S
CS
Reed
R L
RL
Miller
T A
TA
D000758:Anesthesia; D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification; D018407:Bacterial Physiological Phenomena; D001783:Blood Flow Velocity / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects; D004926:Escherichia coli / Q000302:isolation & purification / Q000502:physiology; D006493:Heparin / Q000494:pharmacology; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D008643:Mesentery; D008733:Methoxyflurane / Q000494:pharmacology; D010424:Pentobarbital / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology; D013210:Staphylococcus / Q000302:isolation & purification / Q000502:physiology
D008733:Methoxyflurane; D006493:Heparin; D010424:Pentobarbital