Glutathione concentration during maturation and after fertilization in pig oocytes: relevance to the ability of oocytes to form male pronucleus.
The present study examined the kinetics of glutathione (GSH) concentration during maturation and after fertilization in pig oocytes and its relevance to the ability of pig oocytes to form a male pronucleus after in vitro fertilization. The GSH concentration was significantly higher in pig oocytes matured in Waymouth medium than in pig oocytes matured in either modified (m) TCM-199 or mTLP media. The addition of 0.04-0.57 mM cysteine (CySH) to mTLP significantly increased both the GSH concentrations in oocytes matured in vitro and the rate of male pronucleus formation as compared to those in oocytes cultured in mTLP alone. When pig oocytes were cultured 12, 24, or 36 h in mTLP plus 0.14 mM CySH, their GSH concentrations were significantly higher than in uncultured oocytes. After fertilization, the GSH concentration in pig oocytes declined significantly. GSH concentrations in oocytes matured in vivo did not differ from those in oocytes matured in mTLP plus 0.14 or 0.57 mM CySH. The results indicate that 1) the composition of maturation medium affects the GSH concentration in pig oocytes; 2) the addition of CySH to maturation medium permits GSH synthesis by the pig oocytes; 3) GSH levels in pig oocytes change during maturation and after fertilization; and 4) GSH synthesis during oocyte maturation is an important factor for promoting their ability to form a male pronucleus after fertilization.
Yoshida
M
M
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Ishigaki
K
K
Nagai
T
T
Chikyu
M
M
Pursel
V G
VG
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003470:Culture Media; D003545:Cysteine / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013552:Swine; D015053:Zygote / Q000378:metabolism
D003470:Culture Media; D005978:Glutathione; D003545:Cysteine