title: A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA.
abstract: Zinc fingers of the TFIIIA type are connected by short linker sequences between the structural units. Structural investigations by 2D NMR in solution and by X-ray crystallographic analyses of complexes with DNA point to a passive role for the linkers. We have therefore investigated the influence of the linker sequence on DNA binding using as a model the first three fingers of the protein TFIIIA. Insertion of certain heterologous linkers abolishes binding, and replacement of individual amino acids can reduce binding by factors of up to twenty-four.
authors.affiliation: Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: Y
authors.initials: Y
authors.lastname: Choo
authors.forename: A
authors.initials: A
authors.lastname: Klug
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D014176:Protein Biosynthesis; D013329:Structure-Activity Relationship; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014961:X-Ray Diffraction; D016335:Zinc Fingers
chemical_list: D035582:Transcription Factor TFIIIA; D014157:Transcription Factors; D004247:DNA