title: The effects of gonadectomy and testosterone treatment on the Harderian gland of the green frog, Rana esculenta.
abstract: The effects of gonadectomy and testosterone treatment on the fine structure of the Harderian gland in male and female green frogs were investigated in different periods of the year. Gonadectomy, carried out when the glands are in the lowest secretory phase (September), causes degenerative changes consisting of a reduction of the rough endoplasmic reticulum, the appearance of autolysosomes, and an increase of nuclear heterochromatin. These effects can be prevented by testosterone treatment. No castration effects are found during the recovery (November) and enhancement (April-May) phases of secretory activity. The results suggest that the frog Harderian gland's sensitivity to testosterone changes during the annual cycle. The androgen dependence of the Harderian gland is correlated with the presence of androgen receptors in both male and female frogs.
authors.affiliation: Istituto di Anatomia Umana Normale, Universitá di Napoli, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Chieffi-Baccari
authors.forename: L
authors.initials: L
authors.lastname: D'Matteo
authors.forename: M
authors.initials: M
authors.lastname: d'Istria
authors.forename: S
authors.initials: S
authors.lastname: Minucci
authors.forename: I
authors.initials: I
authors.lastname: Serino
authors.forename: B
authors.initials: B
authors.lastname: Varriale
mesh_terms: D000818:Animals; D005260:Female; D006243:Harderian Gland / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009919:Orchiectomy; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology; D011893:Rana esculenta / Q000033:anatomy & histology*; D012621:Seasons; D013737:Testis / Q000502:physiology; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D013739:Testosterone