p54c-ets-1 and p68c-ets-1, the two transcription factors encoded by the c-ets-1 locus, are differentially expressed during the development of the chick embryo.
The chicken c-ets-1 proto-oncogene encodes two transcription factors, p54c-ets-1 and p68c-ets-1, which contain the same DNA-binding domain but differ in their transactivating activities. We have investigated the spatial and temporal distribution of the transcripts encoding p54c-ets-1 and p68c-ets-1 throughout the development of the chick embryo. We report that p68c-ets-1 as well as p54c-ets-1 is expressed in a wide variety of cells of mesodermal origin, including endothelial cells and mesenchymal cells interacting with epithelium. However, whereas p54c-ets-1 transcripts are detected in most cells, p68c-ets-1 transcripts are restricted to a subset of these cells, randomly distributed. In contrast, p54c-ets-1 is expressed in the absence of p68c-ets-1 in T and B lymphocytes. We show that, during erythropoiesis, both p68c-ets-1 and p54c-ets-1 are expressed in immature erythroid cells in extraembryonic blood islands. The pattern of expression of p54c-ets-1 and p68c-ets-1 during embryonic development suggests the involvement of these transcription factors in the regulation of morphogenetic processes. In addition, we provide the first clue that p68c-ets-1, the cellular progenitor of the v-ets oncogene, is expressed in erythroid cells. This result is very important with respect to the properties of the v-ets oncogene, which confers on the retrovirus E26 the ability to transform erythroid cells.
Quéva
C
C
CNRS URA1160, Institut Pasteur, Lille, France.
Leprince
D
D
Stéhelin
D
D
Vandenbunder
B
B
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D004727:Endothelium / Q000378:metabolism; D005241:Feathers / Q000166:cytology; D015870:Gene Expression; D006410:Hematopoiesis; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000276:immunology; D050783:Proto-Oncogene Proteins c-ets; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors
D009838:Oligodeoxyribonucleotides; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D014157:Transcription Factors