title: Greater susceptibility to mutations in lagging strand of DNA replication in Escherichia coli than in leading strand.
abstract: Models of DNA replication in Escherichia coli involve an asymmetric DNA polymerase complex that replicates concurrently the leading and the lagging strands of double-stranded DNA. The effect of asymmetry on mutagenesis was tested with pairs of plasmids containing the unidirectional ColE1 origin of replication and a single lesion located in the leading or lagging strand. The lesion used was the covalent adduct that the chemical carcinogen N-2-acetylaminofluorene (AAF) forms with the C-8 position of guanine. Whether SOS was induced or not, mutations arose at about a 20-fold higher frequency when the AAF adduct was located in the lagging strand than when in the leading strand.
authors.affiliation: Unité Propre de Recherche Cancérogenèse et Mutagenèse Moléculaire et Structurale, Institut de Biologie Moléculaire et Cellulaire Centre National de la Recherche Scientifique, Strasbourg, France.
authors.forename: X
authors.initials: X
authors.lastname: Veaute
authors.forename: R P
authors.initials: RP
authors.lastname: Fuchs
mesh_terms: D015073:2-Acetylaminofluorene / Q000378:metabolism / Q000633:toxicity; D001483:Base Sequence; D004249:DNA Damage; D004256:DNA Polymerase I / Q000378:metabolism; D004258:DNA Polymerase III / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D016368:Frameshift Mutation; D006147:Guanine / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids
chemical_list: D004269:DNA, Bacterial; D006147:Guanine; D015073:2-Acetylaminofluorene; D004256:DNA Polymerase I; D004258:DNA Polymerase III