title: Kerato-dermal grafts: the importance of dermis for the in vivo growth of cultured keratinocytes.
abstract: In a porcine model, we studied the benefit of dermis for the growth of cultured autologous keratinocytes (CAK) on full-thickness wounds isolated within skin graft chambers. Kerato-dermal grafts were prepared in a two stage process using autologous de-epidermalised dermis (DED) and CAK (Group 1). Control wounds were prepared by grafting either CAK only (Group 2) or DED only (Group 3). The median epidermal cover of 34 wounds in Group 1 was 47% and was significantly greater (p < 0.001) than the epidermal cover of 12 wounds in Group 2 (4%) and 14 wounds in Group 3 (12%). The epidermis in Group 1 was durable whereas it was fragile in the control wounds. Histologically rête ridges were present at 2 weeks in Group 1, but not in the control wounds. These data indicate that a dermal wound bed significantly improves the in vivo growth of cultured keratinocytes.
authors.affiliation: RAFT, Department of Research in Plastic Surgery, Mount Vernon Hospital NHS Trust, Northwood, Middx., UK.
authors.forename: T
authors.initials: T
authors.lastname: Kangesu
authors.forename: H A
authors.initials: HA
authors.lastname: Navsaria
authors.forename: S
authors.initials: S
authors.lastname: Manek
authors.forename: P R
authors.initials: PR
authors.lastname: Fryer
authors.forename: I M
authors.initials: IM
authors.lastname: Leigh
authors.forename: C J
authors.initials: CJ
authors.lastname: Green
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D004817:Epidermis / Q000648:ultrastructure; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000637:transplantation* / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010865:Pilot Projects; D012867:Skin / Q000737:chemistry; D012879:Skin Physiological Phenomena; D016038:Skin Transplantation / Q000502:physiology*; D013552:Swine
chemical_list: D003094:Collagen