Purification and characterization of restriction endonuclease MgoI from Mycobacterium gordonae.
A restriction endonuclease, MgoI, an isoschizomer of Sau3AI, was purified from Mycobacterium gordonae TRC1318. As compared to Sau3AI, the yield of MgoI was seven-eightfold higher, the enzyme was four times more stable at 37 degrees C, and in addition, had optimal activity over a much broader range of salt concentrations.
Shankar
S
S
Department of Biochemistry, University of Delhi South Campus, India.
Tyagi
A K
AK
D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000201:enzymology*
D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases