In vitro and in vivo comparative studies on immunosuppressive properties of cyclosporines A, C, D and metabolites M1, M17 and M21.
Cyclosporine A (CsA) and its major metabolites: M1, M17 and M21 and two analogues: cyclosporines C (CsC) and D (CsD), were studied for their capacity to interfere with different in vitro activation pathways. Their inhibition potentials against the reaction of Graft-versus-Host (GvH) were also studied. The results showed: CsA, CsC and metabolite M17 were the most active compounds upon the inhibition of lymphocyte proliferation induced by different mitogens (ConA, PHA, PWM) and also on the proliferation of mixed lymphocyte cultures (MLC). The same results were observed concerning the direct activation by protein kinase C using a combined action of phorbol ester + calcium ionophore. In vivo using local GvH reaction, CsA and CsC proved more active than M17 in the two different combinations: H-2d --> (H-2b x H-2d)F1 and H-2k --> (H-2b x H-2k)F1 CsD and two metabolites M1 and M21 showed no or weak immunosuppressive effects. Overall, the immunosuppressive potency of six compounds could be schematized as: CsA > or = CsC > M17 > M1 > or = CsD > M21.
Sadeg
N
N
Laboratoire de Toxicologie, Faculté de Pharmacie, Paris, France.
Pham-Huy
C
C
Rucay
P
P
Righenzi
S
S
Halle-Pannenko
O
O
Claude
J R
JR
Bismuth
H
H
Duc
H T
HT
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D016572:Cyclosporine / Q000378:metabolism / Q000494:pharmacology; D003524:Cyclosporins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006087:Graft vs Host Reaction / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D007519:Isoantigens; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D013329:Structure-Activity Relationship
D003524:Cyclosporins; D007166:Immunosuppressive Agents; D007519:Isoantigens; D008934:Mitogens; D000001:Calcimycin; C100000:cyclosporin C; D016572:Cyclosporine; C050026:cyclosporin D