Annexins from bovine adrenal cortex exhibit specific cytosol/membrane solvation.
Cytosol/membrane localization of annexins I to VI was analyzed in tissue extracts from bovine adrenal cortex. Based on their solubility in either aqueous or detergents solutions, they were subfractionated in three groups named cytosolic (C), membrane-bound (MB) and membrane-inserted (MI). Less than 1% of the total annexins present in the tissue were recovered in the C fraction when as much as 76.5 and 22.5% were obtained respectively in the MB and the MI fractions. By immunoblotting after SDS-PAGE, it was shown that the various members of the annexin family were not equally recovered in the different fractions. A-V and A-VI were found present in the three fractions whereas the distribution of A-I, A-II, A-III and A-IV was distinct, suggesting different cellular functions.
Moufquia
J
J
Laboratoire des Etats Liés Moléculaires, Université René Descartes, Paris, France.
Rothhut
B
B
Rainteau
D
D
Comera
C
C
Alfsen
A
A
Russo-Marie
F
F
Lavialle
F
F
D000302:Adrenal Cortex / Q000378:metabolism*; D000818:Animals; D017302:Annexins / Q000302:isolation & purification*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D013347:Subcellular Fractions / Q000378:metabolism
D017302:Annexins