Concentration of fish enveloped viruses from large volumes of water.
The validity of several concentration procedures for the detection of fish enveloped viruses present in large volumes of water was determined. Viral haemorrhagic septicaemia virus (VHSV) was used to evaluate adsorption/elution to positively-charged MK filter cartridges for the concentration of enveloped viruses. For fresh water, the efficiency of the procedure ranged from 12 to 100%, with a mean recovery of 57%. In seawater samples, the recoveries varied from 15 to 100%, with a mean recovery of 59%. The same virus was used in methods such as organic flocculation and ammonium sulphate flocculation with very poor recoveries of infectious virus, caused by the inactivation of VHSV in both procedures. Concentration of seawater samples from tanks housing sea bass or gilthead affected by viral erythrocytic infection and lymphocystis, respectively, were carried out. In both cases, the viruses responsible for the outbreaks were detected by electron microscopy in the concentrated water samples.
Pintó
R M
RM
Department of Microbiology, University of Barcelona, Spain.
Jofre
J
J
Abad
F X
FX
González-Dankaart
J F
JF
Bosch
A
A
D000327:Adsorption; D000818:Animals; D005069:Evaluation Studies as Topic; D005374:Filtration; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes / Q000382:microbiology; D005427:Flocculation; D005618:Fresh Water; D007150:Immunohistochemistry; D007497:Iridoviridae / Q000302:isolation & purification; D016253:Microscopy, Immunoelectron; D012209:Rhabdoviridae / Q000302:isolation & purification; D012623:Seawater; D014461:Ultracentrifugation; D014780:Viruses / Q000302:isolation & purification*; D014871:Water Microbiology