title: Dietary ethanol stimulates the activity of phosphatidylcholine-specific phospholipase D and the formation of phosphatidylethanol in Drosophila melanogaster larvae.
abstract: When administered in the diet to third instar Drosophila melanogaster larvae, short chain primary alcohols reduce phosphatidylcholine (PC) levels. The ethanol-induced reductions in larval PC may be in part due to an increase in the activity of PC-specific phospholipase D (PC-specific PLD, EC 3.1.4.4). PC-specific PLD not only hydrolyzes PC, but it also apparently catalyzes the formation of phosphatidylethanol. PC-specific PLD activity was also stimulated by 200 mM ethanol, methanol, isopropanol, n-butanol, and n-propanol. In vitro studies indicated that Drosophila PC-specific PLD activities were enhanced by submicromolar concentrations of Ca2+ and by GTP-gamma S. In vivo studies utilizing [14C]lyso-palmitoyl phosphatidylcholine indicated that dietary ethanol promoted the flux of label into triacylglycerol, 1,2 diacylglycerol, and fatty acid ethyl esters, while the label in PC decreased.
authors.affiliation: Department of Biology, Knox College, Galesburg, IL 61401.
authors.forename: R R
authors.initials: RR
authors.lastname: Miller
authors.forename: J W
authors.initials: JW
authors.lastname: Yates
authors.forename: B W
authors.initials: BW
authors.lastname: Geer
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D020404:Glycerophospholipids; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006868:Hydrolysis; D007814:Larva; D050356:Lipid Metabolism; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010713:Phosphatidylcholines / Q000378:metabolism*; D010739:Phospholipase D / Q000187:drug effects* / Q000378:metabolism; D010738:Type C Phospholipases / Q000187:drug effects
chemical_list: D020404:Glycerophospholipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; C051521:phosphatidylethanol; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000431:Ethanol; D010738:Type C Phospholipases; D010739:Phospholipase D; D002118:Calcium