Detection of Leishmania RNA virus 1 proteins.
Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.
Cadd
T L
TL
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Keenan
M C
MC
Patterson
J L
JL
D000818:Animals; D015153:Blotting, Western; D002213:Capsid / Q000032:analysis; D007891:Leishmania / Q000382:microbiology*; D007892:Leishmania braziliensis / Q000382:microbiology; D008970:Molecular Weight; D016366:Open Reading Frames; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins