Production of endothelium-derived relaxing factor (EDRF) is compromised after ischemia and reperfusion.
Rabbit hind limbs were subjected to 5 hours of ischemia with or without 1 hour of in vivo reperfusion, and the response of femoral artery segments to vasocontrictors and vasodilators was studied in vitro. The ability of endothelium-derived relaxing factor (EDRF)-dependent vasodilators to relax arterial segments was not altered after up to 5 hours of ischemia alone but was significantly decreased after 4 hours of ischemia if followed by 1 hour of in vivo reperfusion. Thus, we concluded that a specific injury occurs to endothelial cells after ischemia-reperfusion, which occurs solely during the reperfusion period.
Summers
S T
ST
Department of Surgery, UCLA Medical Center.
Zinner
M J
MJ
Freischlag
J A
JA
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004730:Endothelium, Vascular / Q000473:pathology; D005263:Femoral Artery / Q000187:drug effects; D006614:Hindlimb / Q000098:blood supply*; D007511:Ischemia / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D009638:Norepinephrine / Q000494:pharmacology; D011817:Rabbits; D015424:Reperfusion; D014664:Vasodilation / Q000187:drug effects
D009569:Nitric Oxide; D000109:Acetylcholine; D009638:Norepinephrine