Recognition of a palindromic DNA sequence by ESF-1, a factor stimulating transcription of the adenovirus type 12 E1A gene.
We previously identified a factor, designated E1A-stimulating factor 1 (ESF-1), that stimulates transcription of the adenovirus type 12 E1A gene in vitro by binding to a region next to a TATA box. We report here on the partial characterization of the mode of ESF-1 action. Point mutations within the nucleotide sequence 5'-TCAGCTGA-3' abolished the binding of ESF-1. Stimulation of transcription of the adenovirus type 12 E1A gene in vitro was lost when a 7 base pair insertion was introduced between the ESF-1-binding site and a TATA box. These results suggest that ESF-1 stimulates adenovirus type 12 E1A gene transcription by interacting with a palindromic DNA sequence present in a region immediately upstream from a TATA box.
Shibata-Sakurai
H
H
Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.
Masamune
Y
Y
Nakanishi
Y
Y
D017500:Adenovirus E1A Proteins / Q000235:genetics*; D001483:Base Sequence; D001667:Binding, Competitive; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D017354:Point Mutation; D016385:TATA Box; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
D017500:Adenovirus E1A Proteins; D004268:DNA-Binding Proteins; C072241:ESF-1 protein, adenovirus; D009838:Oligodeoxyribonucleotides; D015534:Trans-Activators; D014764:Viral Proteins; D004247:DNA