The gene encoding IFP 53/tryptophanyl-tRNA synthetase is regulated by the gamma-interferon activation factor.
We have obtained genomic DNA encoding the interferon-gamma (IFN-gamma)-inducible IFP 53/tryptophanyl-tRNA synthetase. Comparison with several different IFP 53 cDNA clones revealed a complex pattern of alternatively spliced 5'-untranslated regions. The interferon-responsive region within the IFP 53 promoter was found to contain a gamma-interferon activation site (GAS) but not the interferon-stimulated response element and to bind the gamma-interferon activation factor (GAF). GAF.GAS complexes contained the IFN-regulated 91-kDa protein. Competition experiments defined the GAS boundaries and showed that GAF binding to the IFP 53 GAS could be prevented by an excess of the IFN-gamma response regions of several other IFN-gamma-inducible genes. We thus provide evidence for a central role of GAS.GAF in gene transcription mediated by IFN-gamma and suggest a consensus sequence defining more precisely the requirements for GAF binding to DNA.
Strehlow
I
I
Fraunhofer Institute for Toxicology and Molecular Biology, Hannover, Germany.
Seegert
D
D
Frick
C
C
Bange
F C
FC
Schindler
C
C
Böttger
E C
EC
Decker
T
T
D001483:Base Sequence; D001667:Binding, Competitive; D004247:DNA; D015971:Gene Expression Regulation, Enzymologic; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D015183:Restriction Mapping; D014369:Tryptophan-tRNA Ligase / Q000235:genetics* / Q000378:metabolism
D007371:Interferon-gamma; D004247:DNA; D014369:Tryptophan-tRNA Ligase