Expression and cloning of migration inhibitory factor-related protein (MRP)8 and MRP14 in arthritis-susceptible rats.
Rat migration inhibitory factor-related protein 8 (MRP8) and MRP14 were identified during screening of a subtracted cDNA library generated to identify differences in gene expression between LEW/N and F344/N rats. The predicted amino acid sequence of rat MRP8 and MRP14 is 60-80% identical with that from human and mouse. Expression of these genes correlated with chronic inflammation in LEW/N rats, but were absent in F344/N rats which do not develop arthritis in response to streptococcal cell wall peptidoglycan-polysaccharide complexes (SCW). These differences suggest a role for MRP8 and MRP14 in susceptibility to SCW-induced chronic disease.
Imamichi
T
T
Cellular Immunology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Uchida
I
I
Wahl
S M
SM
McCartney-Francis
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001169:Arthritis, Experimental / Q000235:genetics*; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D040501:Calgranulin A; D040502:Calgranulin B; D003001:Cloning, Molecular; D004247:DNA; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D012210:Rhamnose; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D004247:DNA; D012210:Rhamnose