Sperm antigens and reactivity of antisperm monoclonal antibodies in ELISA.
Several types of sperm antigenic suspensions as well as the whole sperm, either methanol-fixed or air-dried, were checked for intensity of binding to monoclonal antisperm antibodies with known characteristics of reactivity to sperm. The activity of sperm antigen--antibody binding was measured by ELISA (enzyme-linked immunosorbent assay) and compared in several variations (parallely run) of the assay where different types of sperm antigen preparations were applied. The obtained results were then evaluated for statistical significance in Wilcox test. It was shown that antibody reactivity was markedly higher in experiments where the whole sperm was coated in a solid-phase in comparison to results obtained with adhered different sperm antigenic suspensions. However, one exception was noted, where the results from ELISA, run with sperm organic extract, were (statistically) insignificantly lower than those obtained with the whole sperm. Therefore, organic sperm extracts (containing mostly glycolipids) can be a valuable alternative to screening for antisperm antibody activity and/or infertility background.
Kurpisz
M
M
Institute of Human Genetics, Polish Academy of Science, Poznan.
Dobratz
B
B
Alexander
N J
NJ
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D002457:Cell Extracts / Q000276:immunology; D003902:Detergents; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005404:Fixatives; D006801:Humans; D008297:Male; D017830:Octoxynol; D011092:Polyethylene Glycols; D013094:Spermatozoa / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D002457:Cell Extracts; D003902:Detergents; D005404:Fixatives; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40