Enrichment of estradiol receptor from calf uterus.
A three-step procedure to enrich estradiol receptor protein from calf uterus nearly 30,000-fold has been described. Over-all yield is 12%. Control of the single steps has been performed by sodium dodecyl sulfate-electrophoresis, sucrose density gradient centrifugation, gel filtration and determination of receptor quantity. Immunological properties of the preparation obtained have not been controlled.
Dietrich
W
W
Central Institute for Cancer Research, Berlin, Germany.
Wand
H
H
Helbing
D
D
Görlich
M
M
D000645:Ammonium Sulfate; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D011959:Receptors, Estradiol / Q000032:analysis / Q000302:isolation & purification*; D014599:Uterus / Q000737:chemistry*
D011959:Receptors, Estradiol; D000645:Ammonium Sulfate