Evaluation of a modified IRMA for anti-D quantitation, using 3H protein A.
A modified immunoradiometry assay (IRMA) using tritiated (3H) protein A was developed to estimate anti-D concentration. The main advantages of the assay were longer shelf life of the labelled reagent (more than two years); minimum radiation hazard and; low non specific binding. Levels of anti-D were estimated in 23 Rh (D) immunised women. A good correlation of anti-D concentration (micrograms/ml) with Rh antibody titre was observed (r = +0.89, P < 0.01). Anti-D concentration estimated by 3H protein A IRMA correlated well with the severity of Rh-HDN. This assay could quantitate anti-D in sera having exclusively IgG3 subtype.
Dumasia
A
A
Institute of Immunohaematology, Bombay.
Gupte
S
S
D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D015592:Immunoradiometric Assay; D007518:Isoantibodies / Q000097:blood*; D012203:Rh Isoimmunization; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D013205:Staphylococcal Protein A; D014316:Tritium
D007074:Immunoglobulin G; D007518:Isoantibodies; C033242:RH-antibodies; D012204:Rh-Hr Blood-Group System; D013205:Staphylococcal Protein A; D014316:Tritium