title: Subunit composition, crystallization and preliminary crystallographic studies of the Desulfovibrio gigas aldehyde oxidoreductase containing molybdenum and [2Fe-2S] centers.
abstract: The Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe-2S] centers. The enzyme was characterized by SDS/PAGE, gel-filtration and analytical ultracentrifugation experiments. It was crystallized at 4 degrees C, pH 7.2, using isopropanol and MgCl2 as precipitants. The crystals diffract beyond 0.3-nm (3.0-A) resolution and belong to space group P6(1)22 or its enantiomorph, with cell dimensions a = b = 14.45 nm and c = 16.32 nm. There is one subunit/asymmetric unit which gives a packing density of 2.5 x 10(-3) nm3/Da (2.5 A3/Da), consistent with the experimental crystal density, rho = 1.14 g/cm3. One dimer (approximately 2 x 100 kDa) is located on a crystallographic twofold axis.
authors.affiliation: Istituto de Tecnologia Química e Biológica, Oeiras, Portugal.
authors.forename: M J
authors.initials: MJ
authors.lastname: Romão
authors.forename: B A
authors.initials: BA
authors.lastname: Barata
authors.forename: M
authors.initials: M
authors.lastname: Archer
authors.forename: K
authors.initials: K
authors.lastname: Lobeck
authors.forename: I
authors.initials: I
authors.lastname: Moura
authors.forename: M A
authors.initials: MA
authors.lastname: Carrondo
authors.forename: J
authors.initials: J
authors.lastname: LeGall
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: J J
authors.initials: JJ
authors.lastname: Moura
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry*; D000595:Amino Acid Sequence; D003460:Crystallization; D003901:Desulfovibrio / Q000201:enzymology*; D007506:Iron-Sulfur Proteins / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D008982:Molybdenum / Q000032:analysis*; D014961:X-Ray Diffraction
chemical_list: D007506:Iron-Sulfur Proteins; D008982:Molybdenum; D000445:Aldehyde Oxidoreductases