title: Early effects of aldosterone on the basolateral potassium conductance of A6 cells.
abstract: Aldosterone increases the basolateral conductance in target epithelia. The basolateral membrane of tight epithelia contains two different types of K+ conductances (GK), a "resting" and a volume-activated GK. We have studied the early effects (at 4 hours) of 500 nmol/l aldosterone on the basolateral membrane GK of A6 cells (a Xenopus laevis kidney cell line), after the permeabilization of the apical membrane with amphotericin B. In the presence of a 97 to 3 mmol/l apical to basolateral K+ gradient, the "resting", inward rectifying GK was similar in control and aldosterone treated cells. In contrast, aldosterone induced a 2-fold increase of the volume-activated quinidine sensitive GK.
authors.affiliation: Institut de Pharmacologie, Université de Lausanne, Switzerland.
authors.forename: M C
authors.initials: MC
authors.lastname: Broillet
authors.forename: A
authors.initials: A
authors.lastname: Berger
authors.forename: J D
authors.initials: JD
authors.lastname: Horisberger
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D004553:Electric Conductivity; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D007668:Kidney / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011802:Quinidine / Q000494:pharmacology; D014982:Xenopus laevis
chemical_list: D015221:Potassium Channels; D000450:Aldosterone; D000666:Amphotericin B; D011802:Quinidine