Characterization of agarose as an encapsulation medium for particulate specimens for transmission electron microscopy.
Agarose, agar, and gelatin were initially compared as encapsulation media for 3 structurally diverse particulate specimens: bacteria, yeast, and mitochondria. Agarose proved superior to both gelatin and agar for ease of handling and overall image quality (minimum background). All sample types exhibited high quality fixation and structural detail with no heat damage from the agarose medium. Based on this finding, we further characterized agarose encapsulation as affected by post-fixation, en bloc staining and resin type. Osmium tetroxide post-fixation, followed by en bloc uranyl acetate staining, could be performed without an increase in the electron density of the encapsulation medium. Agarose proved successful as an encapsulation medium regardless of the resin type or preparation protocol, thus providing flexibility in experimental design and excellent results over a range of variables.
Wood
J I
JI
Center for Electron Optics, Michigan State University, East Lansing 48824.
Klomparens
K L
KL
D000818:Animals; D005780:Gelatin; D006094:Gram-Positive Bacteria / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*; D012685:Sepharose; D016610:Tissue Embedding; D016707:Tissue Fixation
D005780:Gelatin; D012685:Sepharose