title: Computerized measurement of ciliary beat frequency.
abstract: We describe how ciliary beat frequency (CBF) of human respiratory epithelium in vitro can be robustly and quickly measured. The proven photometric technique has been improved by using a microcomputer to calculate and display the results. The software, which runs on an IBM-compatible PC, was developed using Borland Turbo Pascal and employs a fast Fourier transform routine to derive the CBF. Results are displayed almost as soon as a cell is identified under the microscope, and are in accordance with previous work. The technique can now be used diagnostically and for a variety of drug trials on respiratory epithelium.
authors.affiliation: Department of Medical Physics and Biomedical Engineering, Queen Elizabeth Hospital, Birmingham, UK.
authors.forename: A
authors.initials: A
authors.lastname: Green
authors.forename: P R
authors.initials: PR
authors.lastname: Austin
authors.forename: A
authors.initials: A
authors.lastname: Logan
authors.forename: L A
authors.initials: LA
authors.lastname: Smallman
authors.forename: A B
authors.initials: AB
authors.lastname: Drake-Lee
mesh_terms: D000328:Adult; D002923:Cilia / Q000502:physiology; D004848:Epithelium / Q000502:physiology; D005583:Fourier Analysis; D006801:Humans; D008838:Microcomputers; D012016:Reference Values; D012143:Respiratory Physiological Phenomena; D012137:Respiratory System / Q000503:physiopathology; D012907:Smoking / Q000503:physiopathology; D012984:Software; D014584:User-Computer Interface