title: Immunoglobulins in stimulated tears of dogs.
abstract: Immunoelectrophoresis and single radial immunodiffusion were used to identify and measure tear immunoglobulin concentrations in 50 healthy dogs. Immunoglobulin A and IgG were detected in all samples analyzed, whereas IgM was not detected in any sample. Mean IgA concentration was 25.28 +/- 1.9 mg/dl, adult dogs (> 18 months) having significantly higher mean value. The IgA concentration related to age had significant (P < 0.006) positive correlation; mean IgG concentration was 23.10 +/- 1.72 mg/dl. Linear correlation analysis revealed significant (P < 0.0007) correlation coefficient between tear total protein and IgA concentrations. The IgA and IgG concentrations also were significantly (P < 0.0001) correlated when expressed as milligrams per 100 mg of protein. Relation with sex was not established for either immunoglobulin.
authors.affiliation: Department of Veterinary Clinical Pathology, College of Veterinary Medicine, University of Córdoba, Spain.
authors.forename: P J
authors.initials: PJ
authors.lastname: Ginel
authors.forename: M
authors.initials: M
authors.lastname: Novales
authors.forename: M
authors.initials: M
authors.lastname: García
authors.forename: E M
authors.initials: EM
authors.lastname: Martín
authors.forename: R
authors.initials: R
authors.lastname: López
authors.forename: J M
authors.initials: JM
authors.lastname: Molleda
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D004285:Dogs / Q000276:immunology*; D005260:Female; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000032:analysis* / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D008297:Male; D012737:Sex Factors; D013666:Tears / Q000276:immunology*
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G