[IgE, IgG1 and IgG4 antibodies against bodily and fecal extracts of Dermatophagoides farinae].
IgE, IgG1 and IgG4 antibodies against bodily (Dfb) and fecal (Dff) extracts of Dermatophagoides farinae (Df) were measured by enzyme linked immunosorbent assay (ELISA) in 33 asthmatic patients (AP), 27 healthy control subjects (HCS) and clinically healthy subjects with mite allergy (HS-MA) shown by positive skin reactions to house dust. Serum total IgE measured by radioimmunosorbent test, and IgE against Df was measured by the CAP system simultaneously. AP showed the highest mean value of total IgE and IgE against Df, Dfb and Dff. HS-MA showed the second highest value and HCS showed the lowest one. There were significant differences among these 3 groups. AP showed a significantly higher mean value of IgG1 and IgG4 than the other 2 groups. However, there was no significant difference between HS-MA and HCS. Antibody titers against Dfb and Dff correlated well. IgE, IgG1 and IgG4 titers obtained by ELISA also correlated with each other well. However, the results from the CAP system and IgE-ELISA did not agree in some cases indicating character differences between the CAP system and ELISA. In AP, the CAP system results agreed with those of ELISA. In HS-MA, however, many cases exhibited negative reactions to IgE-ELISA in spite of exhibiting positive reactions in the CAP system.
Yasumatsu
Y
Y
Second Department of Internal Medicine, Hiroshima University School of Medicine.
D000328:Adult; D000818:Animals; D001249:Asthma / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology*
D007074:Immunoglobulin G; D007073:Immunoglobulin E