title: Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation.
abstract: The cistronic organization of the bph locus, encoding a biphenyl/polychlorinated biphenyl (PCB) degradation pathway in Pseudomonas sp. LB400, has been elucidated. Seven structural genes, encoding biphenyl dioxygenase (bphA1A2A3A4), biphenyl-2,3-dihydrodiol-2,3-dehydrogenase (bphB), biphenyl-2,3-diol-1,2-dioxygenase (bphC) and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD), have been located. The complete sequences of bphB, bphC and bphD are reported. Taken together with the data of Erickson and Mondello [J. Bacteriol. 174 (1992) 2903-2912], Pseudomonas sp. LB400 is now the first strain for which the sequences of all genes encoding the catabolism from biphenyls to benzoates have been determined. Comparisons of the deduced amino acid (aa) sequences of BphB, BphC and BphD with those of related proteins led to predictions about catalytically important aa residues. Six Bph have been detected and identified. Five of them could be obtained as the most abundant proteins when their genes were expressed in Escherichia coli.
authors.affiliation: Department of Microbiology, Gesellschaft für Biotechnologische Forschung, Braunschweig, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Hofer
authors.forename: L D
authors.initials: LD
authors.lastname: Eltis
authors.forename: D N
authors.initials: DN
authors.lastname: Dowling
authors.forename: K N
authors.initials: KN
authors.lastname: Timmis
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001565:Benzoates / Q000378:metabolism; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D049308:Dioxygenases; D015870:Gene Expression; D005798:Genes, Bacterial; D006867:Hydrolases / Q000235:genetics*; D007506:Iron-Sulfur Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D010105:Oxygenases / Q000235:genetics*; D011078:Polychlorinated Biphenyls / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D001565:Benzoates; D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D007506:Iron-Sulfur Proteins; D011993:Recombinant Fusion Proteins; D011078:Polychlorinated Biphenyls; D010105:Oxygenases; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase; C074161:biphenyl-2,3-dioxygenase; D006867:Hydrolases; C062907:2,6-dioxo-6-phenylhexa-3-enoate hydrolase