title: Bienzyme strip-type glucose sensor.
abstract: A strip-type glucose biosensor, prepared using screen-printing technology and comprising glucose oxidase (E.C.1.1.3.4.), peroxidase (E.C.1.1.3.13.) and ferrocyanide as mediator incorporated into graphite-hydroxyethyl cellulose matrices is described. The sensor acted at 0.0 V vs Ag/AgCl electrode, and the response time was 50-60 s. The calibration was linear up to 25 mM of glucose. The sensor response was constant in the range of pH 7.0-8.5. At 25 degrees C the biosensor temperature coefficient was 2.7% degrees C(-1). The sensor was insensitive to a physiological level of ascorbic acid (40 microM) and was used for glucose determination in whole blood.
authors.affiliation: Institute of Biochemistry, Lithuanian Academy of Sciences, Vilnius-MTP.
authors.forename: J
authors.initials: J
authors.lastname: Marcinkeviciene
authors.forename: J
authors.initials: J
authors.lastname: Kulys
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D004867:Equipment Design; D005949:Glucose Oxidase / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration
chemical_list: D001786:Blood Glucose; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase