Peripheral blood neutrophils from HIV-1-infected individuals are armed with factors that cause inhibition of their migration in response to specific antigens.
In this study, synthetic peptides that copy conserved regions of the HIV-1 envelope proteins gp120 and gp41 were tested for their impact on the chemotaxis of leukocytes and neutrophils from HIV-1-infected individuals, while neutrophils from HIV-1-infected patients were tested for their effect on the chemotaxis of neutrophils from healthy donors. The synthetic peptides (corresponding to the 251-272-amino acid sequence of gp120 and the 584-618-amino acid sequence of gp41) were capable of specifically inhibiting the formyl peptide-induced chemotaxis of cells from HIV-1-infected patients, and such inhibition was observed both with a total leukocyte population and with pure neutrophils. The migration of neutrophils from healthy donors was specifically inhibited in the presence of either of the synthetic peptides of HIV-1 envelope proteins after their incubation with neutrophil supernatants obtained from HIV-1-infected individuals. As shown by ELISA tests, the neutrophil supernatants from HIV-1-infected individuals contain antibodies to a recombinant env-1 protein that might be one of the reasons for the specific arming of neutrophils from HIV-1-infected persons.
Pinegin
B V
BV
Institute of Immunology, Ministry of Health of Russia, Moscow.
Saidov
M Z
MZ
Scheltsyna
T L
TL
Gabrilovich
D I
DI
Khaitov
R M
RM
D000328:Adult; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005814:Genes, Viral / Q000276:immunology; D015483:HIV Antibodies / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology
D015483:HIV Antibodies; D015488:HIV Antigens; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D016056:Immunodominant Epitopes; D010455:Peptides; D009240:N-Formylmethionine Leucyl-Phenylalanine