title: Sheep brain pseudocholinesterase: inhibition kinetics of the partially purified enzyme by some substrate analogues.
abstract: Pseudocholinesterase (ChE) (acylcholineacylhydrolase, EC 3.1.1.8) has been partially purified (about 270-fold) from sheep brain. The procedure included ammonium sulfate fractionation (20-80%), DEAE-Trisacryl M chromatography and procainamide-Sepharose 4B affinity chromatography. The molecular weight of purified ChE was found to be 290,000 by gel filtration. Kinetic properties of the enzyme have been studied using the substrate analogues choline, succinylcholine and benzoylcholine. It was shown that the inhibition was partially competitive.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: A N
authors.initials: AN
authors.lastname: Cokuğraş
authors.forename: E F
authors.initials: EF
authors.lastname: Tezcan
mesh_terms: D000110:Acetylcholinesterase / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001588:Benzoylcholine / Q000378:metabolism / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D002091:Butyrylcholinesterase / Q000302:isolation & purification / Q000378:metabolism*; D002092:Butyrylthiocholine / Q000378:metabolism; D002794:Choline / Q000378:metabolism / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology; D006868:Hydrolysis; D007700:Kinetics; D012756:Sheep; D013390:Succinylcholine / Q000378:metabolism / Q000494:pharmacology
chemical_list: D002800:Cholinesterase Inhibitors; D001588:Benzoylcholine; D002092:Butyrylthiocholine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D013390:Succinylcholine; D002794:Choline