title: Lyophilization as a method to store samples of whole blood.
abstract: We have investigated the feasibility of using lyophilization as a method of preserving whole blood samples for HLA class II molecular typing. Genomic DNA extracted from lyophilized blood appears similar in size to that extracted from fresh or frozen blood and is suitable for both PCR amplification and Southern blot analysis.
authors.affiliation: Department of Pediatrics, Rangos Research Center, Children's Hospital of Pittsburgh, PA 15213.
authors.forename: E P
authors.initials: EP
authors.lastname: Weisberg
authors.forename: R
authors.initials: R
authors.lastname: Giorda
authors.forename: M
authors.initials: M
authors.lastname: Trucco
authors.forename: V
authors.initials: V
authors.lastname: Lampasona
mesh_terms: D001793:Blood Preservation / Q000379:methods*; D015139:Blotting, Southern; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D019286:Endopeptidase K; D005612:Freeze Drying; D005802:Genes, MHC Class II / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D012697:Serine Endopeptidases
chemical_list: D004247:DNA; D012697:Serine Endopeptidases; D019286:Endopeptidase K