Influence of the different amino acid substitutions in Escherichia coli thioredoxin on the growth of bacteriophages T7 and f1.
We have constructed three mutants in the thioredoxin (trxA) gene changing its catalytic core between Cys-32 and Cys-35. Oligonucleotide-directed mutagenesis was carried out to replace conservative Gly-33 or Pro-34 by leucine, lysine, glutamine, phenylalanine or tryptophane. The mutants were characterized using an in vivo assay based on the ability of cell (mutants in the chromosomal trxA gene) to support growth of T7 and filamentous f1 phages. The results indicate that the smaller group side-chain in the position 33 and 34 of amino acid residues are indispensable for the growth of phages.
Minárik
P
P
Department of Biochemistry, Comenius University, Bratislava, Czechoslovakia.
Kollárová
M
M
Brunovská
A
A
D017123:Bacteriophage T7 / Q000254:growth & development; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003090:Coliphages / Q000254:growth & development*; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D013879:Thioredoxins / Q000235:genetics*; D010948:Viral Plaque Assay
D013879:Thioredoxins