title: In vitro and in vivo assays of isopropanol for mutagenicity.
abstract: To assess the mutagenic potential of isopropanol, an in vitro Chinese hamster ovary (CHO) cell/HGPRT gene mutation assay and a bone marrow micronucleus study in mice were conducted. In the CHO/HGPRT assay, concentration levels ranged from 0.5 to 5.0 mg/ml. No elevated mutant frequencies attributable to treatment were observed in the test under either activated or non-activated conditions. In the micronucleus assay, mice were injected intraperitoneally (IP) with either 350, 1,173, or 2,500 mg/kg of isopropanol at constant volumes of 10 ml/kg. No increased incidence of micronuclei was observed in bone marrow polychromatic erythrocytes (PCEs) harvested at 24, 48, or 72 hr post-dosing. In both assays, negative and positive control mutant frequencies were within historical control ranges. These results, in conjunction with previously published data, clearly demonstrate that isopropanol is not a mutagen.
authors.affiliation: Toxicology Division, BioTox, Inc., Richmond, Virginia 23236.
authors.forename: R W
authors.initials: RW
authors.lastname: Kapp
authors.forename: D J
authors.initials: DJ
authors.lastname: Marino
authors.forename: T H
authors.initials: TH
authors.lastname: Gardiner
authors.forename: L W
authors.initials: LW
authors.lastname: Masten
authors.forename: R H
authors.initials: RH
authors.lastname: McKee
authors.forename: T R
authors.initials: TR
authors.lastname: Tyler
authors.forename: J L
authors.initials: JL
authors.lastname: Ivett
authors.forename: R R
authors.initials: RR
authors.lastname: Young
mesh_terms: D000433:1-Propanol / Q000633:toxicity*; D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001711:Biotransformation; D016466:CHO Cells; D020111:Chlorodiphenyl (54% Chlorine); D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000378:metabolism; D016296:Mutagenesis; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity*; D051381:Rats
chemical_list: D001140:Aroclors; D009153:Mutagens; D020111:Chlorodiphenyl (54% Chlorine); D000433:1-Propanol; D006899:Mixed Function Oxygenases; D007041:Hypoxanthine Phosphoribosyltransferase