title: Sulfation of hypertensive and hypotensive drugs by monkey brain phenol sulfotransferase.
abstract: The substrate specificity and affinity of two forms of phenol sulfotransferase (PST) from Rhesus macaque brain cortex were studied. Catecholamines, their methylated metabolites (normetanephrine, metanephrine) and methylated precursor, alpha-methylDOPA, were examined as substrates for both the cationic (PST I) and the anionic (PST II) forms of the enzyme. Sulfation of hypertensive drugs (phenylephrine, octopamine, metaraminol), hypotensive drugs (alpha-methylDOPA, minoxidil), and related agents without a free hydroxy group on the benzene ring were also studied. Results indicated that both PST forms sulfated alpha-methylDOPA and minoxidil, but only PST II transferred the sulfate group to catecholamines and most of the adrenergic agents examined.
authors.affiliation: Department of Biochemistry, Institute of Biopharmacy, Warsaw Medical School, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Barańczyk-Kuźma
authors.forename: D
authors.initials: D
authors.lastname: Drobisz
authors.forename: K L
authors.initials: KL
authors.lastname: Audus
authors.forename: R T
authors.initials: RT
authors.lastname: Borchardt
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000378:metabolism*; D015239:Arylsulfotransferase / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D008253:Macaca mulatta; D008680:Metaraminol / Q000378:metabolism; D008750:Methyldopa / Q000378:metabolism; D008914:Minoxidil / Q000378:metabolism; D009655:Octopamine / Q000378:metabolism; D010656:Phenylephrine / Q000378:metabolism; D013379:Substrate Specificity; D013431:Sulfates / Q000378:metabolism*; D013566:Sympathomimetics / Q000378:metabolism*
chemical_list: D000959:Antihypertensive Agents; D013431:Sulfates; D013566:Sympathomimetics; D009655:Octopamine; D010656:Phenylephrine; D008750:Methyldopa; D008914:Minoxidil; D008680:Metaraminol; D015239:Arylsulfotransferase