title: Renal toxicity of aristolochic acid in rats as an example of nephrotoxicity testing in routine toxicology.
abstract: The nephrotoxic action of aristolochic acid (AA) was investigated in female Wistar rats given single doses of 10, 50 or 100 mg/kg by gastric tube. Renal lesions developed within 3 days, the effect being dose-dependent. Histologically, there was evidence of necrosis of the epithelium of the renal tubules, and functionally, there were rises in plasma creatinine and urea together with increases in urinary glucose, protein, N-acetyl-beta-glucosaminidase, gammaglutamyl transferase and malate dehydrogenase. Taking AA as an example, the aim of the present study was to consider the suitability of this model, based on a combination of histology and laboratory investigations, as a short-term test for the detection of nephrotoxic agents.
authors.affiliation: Toxicology and Experimental Pathology, Madaus AG, Köln, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Mengs
authors.forename: C D
authors.initials: CD
authors.lastname: Stotzem
mesh_terms: D000818:Animals; D034341:Aristolochic Acids; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity*; D003404:Creatinine / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005260:Female; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced* / Q000652:urine; D007677:Kidney Function Tests; D009929:Organ Size / Q000187:drug effects; D010616:Phenanthrenes / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000097:blood
chemical_list: D034341:Aristolochic Acids; D002273:Carcinogens; D010616:Phenanthrenes; D014508:Urea; C000228:aristolochic acid I; D003404:Creatinine; C042310:aristolochic acid II