Virus isolation accuracy: an evaluation of eight laboratories involved in poultry diagnostics.
A study was undertaken to evaluate the accuracy of diagnostic laboratories at isolation of common avian viral pathogens. Fourteen "unknown" samples were submitted to eight laboratories in seven states. All positive and negative samples were guaranteed pure by SPAFAS. Virus-isolation results were erroneous in many cases. Based on this study, it appears that protocol for virus isolation of avian pathogens should be standardized throughout the United States.
Davis
J F
JF
Georgia Poultry Laboratory, Oakwood, Georgia 30566.
Girshick
T
T
Crary
C K
CK
Fredericksen
T L
TL
Brown
J
J
Dickson
T G
TG
D000818:Animals; D004267:DNA Viruses / Q000302:isolation & purification*; D005189:False Positive Reactions; D007753:Laboratories / Q000592:standards*; D011200:Poultry; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D011786:Quality Control; D012328:RNA Viruses / Q000302:isolation & purification*; D014773:Virology / Q000592:standards*; D014777:Virus Diseases / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*