[Automated micro-determination by transfer analyzer of circulating alpha-amino nitrogen].
An automated method for the determination of plasma free amino groups is described. The analysis is performed on a discrete computerized analyzer after plasma deproteinization using trichloracetic acid. The method is based on the formation of a complex between terminal NH2 radicals and 2,4,6-trinitrobenzene sulfonate (TNBS). The method is accurate (coefficient of variation = 2%), linear over a range of 0.2-7 mM/l and requires small analytic volumes; it also correlates well in the bovine with results from standard liquid chromatography (r = 0.953). With this method a single operator can determine free amino group contents of 50 samples per h.
Chacornac
J P
JP
INRA de Clermont-Theix, Laboratoire d'Ecopathologie, Saint-Genès-Champanelle, France.
Barnouin
J
J
Houlier
M L
ML
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D001322:Autoanalysis / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D002417:Cattle; D003124:Colorimetry; D005260:Female; D008832:Microchemistry; D009584:Nitrogen / Q000097:blood*
D000596:Amino Acids; D009584:Nitrogen
Micro-dosage automatisé sur analyseur à transfert de l'azote alpha-aminé circulant.