Functional variation in endogenous and exogenous immunoglobulin binding to bovine neutrophils relative to parturition.
Ten healthy first- and second-lactation Holstein cows were observed from 1 week before to 1 week after calving and at postpartum day 30 to determine polymorphonuclear neutrophil (PMN) functional variation and immunoglobulin binding profiles. Blood and mammary PMN were obtained 3 times weekly and within 24 hours of calving. Functional traits measured included phagocytosis of Staphylococcus aureus and in vitro chemotaxis through micropore filters in a Boyden chamber. Additionally, PMN were evaluated for endogenous binding of IgG1, IgG2, IgA, and IgM before and after in vitro chemotaxis. Exogenous binding of the same isotypes was determined after incubation in pooled colostrum, purified immunoglobulin, and pooled sera. Phagocytosis results indicated a significant and transient increase in percentage of milk PMN with associated, rather than phagocytosed, bacteria for 1 week after calving. Blood PMN phagocytosis was not significantly different during this period. Though total chemotaxis was essentially unchanged, the percentage of PMN that were unable to complete migration increased substantially on the day of calving, an effect that disappeared by postpartum day 4. A significant (P < 0.01) positive correlation (r = 0.29) between percentage of PMN migrating completely through the micropore filter and percentage of blood PMN with associated bacteria was observed. Changes were not observed in endogenous immunoglobulin binding, with the exception of a peak in relative fluorescence intensity for IgG1 on the day of calving; this disappeared within 2 days after calving. Correlations between relative intensities of IgG2 and IgM, and percentage of mammary neutrophils phagocytosing were 0.37 and 0.70.(ABSTRACT TRUNCATED AT 250 WORDS)
Berning
L M
LM
Department of Animal Sciences, University of Maryland, College Park 20740.
Paape
M J
MJ
Peters
R R
RR
D000818:Animals; D002417:Cattle; D002634:Chemotaxis, Leukocyte; D003126:Colostrum / Q000276:immunology; D005260:Female; D007070:Immunoglobulin A / Q000097:blood / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification / Q000378:metabolism; D007132:Immunoglobulin Isotypes / Q000378:metabolism; D007075:Immunoglobulin M / Q000097:blood / Q000378:metabolism; D007136:Immunoglobulins / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D008321:Mammary Glands, Animal / Q000502:physiology; D008892:Milk / Q000276:immunology / Q000502:physiology*; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D010587:Phagocytosis; D049590:Postpartum Period / Q000276:immunology / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology / Q000502:physiology*; D013211:Staphylococcus aureus
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007136:Immunoglobulins