title: Application of flow cytometry to the study of antiphagocytic properties of Klebsiella pneumoniae capsular polysaccharide.
abstract: We used flow cytometry to compare the effects of whole cells and capsular polysaccharides of Klebsiella pneumoniae on the phagocytic ability ot polymorphonuclear leukocytes. Our results showed a light decrease in phagocytic activity in the presence of capsular polysaccharides, but a marked decrease with whole cells. Our findings suggest that the resistance to phagocytosis in these microorganisms is not due exclusively to their capsule, as claimed by other authors.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Ruiz
authors.forename: J M
authors.initials: JM
authors.lastname: Sabio
authors.forename: J L
authors.initials: JL
authors.lastname: Santos
authors.forename: M J
authors.initials: MJ
authors.lastname: Montes
authors.forename: E G
authors.initials: EG
authors.lastname: Olivares
mesh_terms: D005434:Flow Cytometry; D006801:Humans; D007711:Klebsiella pneumoniae / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000502:physiology*
chemical_list: D011135:Polysaccharides, Bacterial