Analysis of asparagine-linked oligosaccharides by sequential lectin-affinity chromatography.
Various immobilized lectins can be successfully used for fractionation and for structural studies of asparagine-linked sugar chains of glycoproteins (see Note 9). This method needs < 10 ng of a radiolabeled oligosaccharide prepared from a glycoprotein by hydrazinolysis or by digestion with endo-beta-N-acetylglucosaminidases. The fractionation and the structural assessment through the use of immobilized lectins make the subsequent structural studies much easier.
Yamamoto
K
K
Tsuji
T
T
Osawa
T
T
D000818:Animals; D001216:Asparagine / Q000032:analysis*; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D037102:Lectins; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry
D037102:Lectins; D009844:Oligosaccharides; D001216:Asparagine