[Characteristics of the pH2-42 probe for the D13S25 locus of human chromosome 13: nucleotide sequence, localization, and PCR markers].
The sequence of the HindIII-HindIII fragment of probe pH2-42 of locus D13S25 of human genome is given. Localization of the probe in q14-q21 of human chromosome 13 is confirmed by hybridization in situ. Seven oligonucleotide primers for the polymerase chain reaction are chosen so that amplified products almost completely cover the analyzed sequence. Reconstruction of localization of polymorphic SspI-sites in D13S25 was based on the data of Bowcock and Hebert [3] and this study. The results obtained make it possible to use the primer sets to screen cosmid libraries and to mark the D13S25 locus of human chromosome 13.
Sulimova
G E
GE
Zakhar'ev
V M
VM
Udina
I G
IG
Bliskovskiĭ
V V
VV
Kirillov
A V
AV
Kapanadze
B I
BI
Shchepinov
M S
MS
Borob'eva
N V
NV
Grafodatskiĭ
A S
AS
Iankovskiĭ
N K
NK
D001483:Base Sequence; D002882:Chromosomes, Human, Pair 13; D003360:Cosmids; D004277:DNA, Single-Stranded; D005819:Genetic Markers; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D004277:DNA, Single-Stranded; D005819:Genetic Markers
Kharakteristika proby pH2-42 lokusa D13S25 13-ĭ khromosomy cheloveka: nukleotidnaia posledovatel'nost', lokalizatsiia, PTsR-markery.