An alternative modality for the immunodetection of the molecular phenotypes of coagulation factor XIIIB.
A new technical modality for the typing of coagulation factor XIIIB (F13B) is described. The determination of F13B phenotypes was carried out from desyalized plasma samples by means of polyacrylamide gel isoelectric focusing (pH range 6-8) followed by immunofixation-silver stain. This method combines high sensitivity, low expenditure, and a single methodology. A genetic analysis on F13B phenotypes in the Galician population is also discussed.
Cameselle
F D
FD
Departamento de Antropoloxía, Facultade de Bioloxía, Universidade de Santiago, España.
Caeiro
B
B
Riveiro
A
A
D000483:Alleles; D005176:Factor XIII / Q000737:chemistry* / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D007120:Immunochemistry; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic; D013030:Spain
D005176:Factor XIII; C054380:factor XIIIb