title: Human monoclonal anti-D-induced clearance of human D-positive red cells in a chimpanzee model.
abstract: Two human monoclonal anti-Rh0(D) antibodies, one IgG1, and one IgG3, were tested for their ability to clear human D-positive red blood cells (RBCs) from chimpanzee circulation. Human RBCs (phenotype A1, R1r) from 1 donor were radiolabelled with chromium 51 and injected into 4 chimpanzees. One day later the control animal received isotonic saline whereas 2 animals received 400 micrograms of purified human monoclonal anti-D, either IgG1 or IgG3. The remaining animal received both antibodies together (200 micrograms of IgG1 and 200 micrograms of IgG3). Both individual antibody-mediated clearance of human D-positive cells and synergy was not observed when both antibodies were used in combination. IgG1 was slightly more effective than IgG3. This animal model is a suitable alternative for conducting in vivo experiments in human beings, especially at the preclinical study phase of monoclonal anti-D antibodies.
authors.affiliation: Laboratoire d'Anthropologie Physique du Collège de France, Paris.
authors.forename: A
authors.initials: A
authors.lastname: Blancher
authors.forename: W W
authors.initials: WW
authors.lastname: Socha
authors.forename: F
authors.initials: F
authors.lastname: Roubinet
authors.forename: A W
authors.initials: AW
authors.lastname: Rowe
authors.forename: H
authors.initials: H
authors.lastname: Broly
authors.forename: P
authors.initials: P
authors.lastname: Byrne
authors.forename: M
authors.initials: M
authors.lastname: Holuigue
authors.forename: A
authors.initials: A
authors.lastname: Bouzidi
authors.forename: J J
authors.initials: JJ
authors.lastname: Huart
authors.forename: J
authors.initials: J
authors.lastname: Ruffié
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002470:Cell Survival / Q000502:physiology; D004912:Erythrocytes / Q000276:immunology*; D006207:Half-Life; D006801:Humans; D007114:Immunization; D007275:Injections, Intravenous; D008954:Models, Biological; D002679:Pan troglodytes / Q000097:blood*; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen