Identification of an operon involved in the assimilatory nitrate-reducing system of Azotobacter vinelandii.
A number of mutants lacking nitrate reductase (Nas-) or nitrite reductase (Nis-) activities have been isolated and characterized. An operon including two new genes (nasA and nasB) has been defined and cloned from an Azotobacter vinelandii gene bank. nasA encodes for nitrite reductase apoenzyme, whereas nasB is specific for nitrate reductase activity. Nitrate reductase exerts a regulatory effect on nasAB.
Ramos
F
F
Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Spain.
Blanco
G
G
Gutiérrez
J C
JC
Luque
F
F
Tortolero
M
M
D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000235:genetics / Q000378:metabolism; D016948:Azotobacter vinelandii / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics* / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009572:Nitrite Reductases / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D010084:Oxidation-Reduction; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D001051:Apoenzymes; D001426:Bacterial Proteins; D009566:Nitrates; D011993:Recombinant Fusion Proteins; D009565:Nitrate Reductases; D009572:Nitrite Reductases; D050901:Nitrate Reductase