A new method for determining anti-B cell antibodies and their specificity using flow cytometry.
We describe a new flow cytometric B cell crossmatch method with improved sensitivity and specificity. It is based on the coating of B cell surface immunoglobulins with an unconjugated polyvalent anti-human immunoglobulin antibody. The method also provides a means for determining the specificity of anti-B cell antibodies and, potentially, for anti-HLA class II antibody specificity differentiation (DR, DQ or DP) in a binding inhibition test using mouse monoclonal antibodies directed against human HLA class II antigens.
Berteli
A J
AJ
Department of Transplantation Immunology, University of Heidelberg, Germany.
Daniel
V
V
Terness
P
P
Opelz
G
G
D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D006681:HLA-D Antigens / Q000276:immunology; D006650:Histocompatibility Testing / Q000295:instrumentation*; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*
D006681:HLA-D Antigens; D007518:Isoantibodies