Retinoic acid induces insulin-like growth factor II expression in a neuroblastoma cell line.
Insulin-like growth factor II (IGF-II) is implicated in the development of the vertebrate neural circuitry, and increases neurite growth in vitro and in vivo. We examined the relationship of IGF-II expression to the in vitro differentiation induced by retinoic acid (RA). We find that RA stimulates an increase in IGF-II messenger RNA (mRNA) in the SK-N-SH (SH) neuroblastoma cell line. An increase of IGF-II mRNA is detected within 12 h of treatment and precedes morphological differentiation. A RA dose response test indicates that an increase in IGF-II mRNA occurs within 2 days in SH cells treated with doses of RA from 1 x 10(-8) to 1 x 10(-5) M. We suggest that IGF-II expression may be regulated either directly or indirectly by RA in vitro and may lead to neuroblastoma differentiation.
Ueno
T
T
Department of Pediatric Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Suita
S
S
Zaizen
Y
Y
D002454:Cell Differentiation; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000187:drug effects; D009447:Neuroblastoma / Q000188:drug therapy; D012333:RNA, Messenger / Q000032:analysis; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*
D012333:RNA, Messenger; D014212:Tretinoin; D007335:Insulin-Like Growth Factor II