title: The conformation of tRNA genes. Chemical modification studies.
abstract: It has been suggested that eukaryotic tRNA genes might adopt a higher order stem and loop structure to facilitate transcription by interaction of their variably spaced intragenic promoter blocks. Using sodium bisulphite, which reacts specifically with cytosine residues in single-stranded nucleic acids, no deamination of C in the TTCGAA sequence of the 3' ICR of a tRNA(Leu) gene could be detected under conditions which caused 60% deamination of cytosine residues within the loop region of a synthetic cruciform cloned in the same negatively supercoiled plasmid vector. We conclude that, under these conditions, such structures occur in tRNA genes very rarely, if at all.
authors.affiliation: Department of Biochemistry, University of Glasgow, Scotland, UK.
authors.forename: A
authors.initials: A
authors.lastname: McLaren
authors.forename: E S
authors.initials: ES
authors.lastname: Gonos
authors.forename: T
authors.initials: T
authors.lastname: Carr
authors.forename: J P
authors.initials: JP
authors.lastname: Goddard
mesh_terms: D004278:DNA, Superhelical / Q000187:drug effects; D006801:Humans; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000235:genetics*; D011995:Recombination, Genetic; D013447:Sulfites / Q000494:pharmacology
chemical_list: D004278:DNA, Superhelical; D012356:RNA, Transfer, Leu; D013447:Sulfites; C005200:sodium metabisulfite