title: Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme.
abstract: The gene encoding the psi subunit of DNA polymerase III holoenzyme, holD, was identified and isolated by an approach in which peptide sequence data were used to obtain a DNA hybridization probe. The gene, which maps to 99.3 centisomes, was sequenced and found to be identical to a previously uncharacterized open reading frame that overlaps the 5' end of rimI by 29 bases, contains 411 bp, and is predicted to encode a protein of 15,174 Da. When expressed in a plasmid that also expressed holC, holD directed expression of the psi subunit to about 3% of total soluble protein.
authors.affiliation: Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: J R
authors.initials: JR
authors.lastname: Carter
authors.forename: M A
authors.initials: MA
authors.lastname: Franden
authors.forename: R
authors.initials: R
authors.lastname: Aebersold
authors.forename: C S
authors.initials: CS
authors.lastname: McHenry
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004258:DNA Polymerase III / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D004258:DNA Polymerase III