[Description and validation of a flow cytometric method to estimate residual leukocytes in leukocyte-depleted red cell concentrates].
We describe a flow-cytometric method for estimating residual white blood cells (WBC) counts in WBC depleted blood components, namely units of packed red cells. The method uses fluorescent staining of nuclear ADN with ethidium bromide. WBC nuclei are discriminated from background events using fluorescence ratio (585 nm versus 650 nm). A facultative procedure of concentration is described, in order to get better sensitivity at very low WBC counts. Main steps of validation are dilution assays and correlation with hemocytometer counts. The method can be used to explore very low concentrations (less than 100 WBC/ml) and should be useful in quality control of blood products.
Lambrey
Y
Y
Centre Départemental de Transfusion Sanguine de Chalon-sur-Saône.
Creuzenet
C
C
Bougy
F
F
Cohen
J H
JH
Biggio
B
B
D004912:Erythrocytes / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D016014:Linear Models; D015203:Reproducibility of Results
Description et validation d'une méthode cytofluorométrique d'estimation des leucocytes résiduels dans les concentrés globulaires déleucocytés.