Accumulation of (E)-4-hydroxy-2-nonenal and n-hexanal, degradation products of lipid peroxides, in mouse lung and liver.
Effects of lipid peroxide breakdown products, (E)-4-hydroxy-2-nonenal (4-HN) and n-hexanal, on mouse lung lesion were examined. When 4-HN was injected i.v., the plasma level of 4-HN increased just after the injection and then decreased immediately. The amounts of 4-HN increased in the liver and lung were ca. 0.085 and 0.43% to the dose administered, respectively, 5 min after the injection. Reduced glutathione (GSH) content and both GSH peroxidase (GSH-Px) and GSH reductase (GSSGR) activities in the lung were decreased significantly by 4-HN treatment. On the other hand, in the case of i.v. injection of n-hexanal into mice, the amount of n-hexanal detected in the lung was 5.0% to that of 4-HN, and no effect on the activities of GSH-Px and GSSGR and the content of GSH was observed. These results suggest that 4-HN generated from lipid peroxides would be transferred into the lung and cause the lung lesion through the inhibition of GSH-dependent antioxidative defense systems.
Yoshino
K
K
Laboratory of Health Science, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Sano
M
M
Hagiwara
M
M
Fujita
M
M
Tomita
I
I
D000447:Aldehydes / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007275:Injections, Intravenous; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000447:Aldehydes; D017392:Thiobarbituric Acid Reactive Substances; C010463:n-hexanal; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; C027576:4-hydroxy-2-nonenal