Adenosine modulates reactive hyperemia in rat gut.
Intestinal reactive hyperemia is an abrupt blood flow increase following release from anterior mesenteric arterial occlusion. We investigated the role of adenosine in reactive hyperemia. In anesthetized rats, mesenteric arterial velocity of blood flow was determined with pulsed Doppler velocimetry and arterial pressure with a transducer. Three indices quantifying reactive hyperemias obtained following 30, 60, and 120 s arterial occlusions included duration, the volume of blood flow exceeding preocclusion blood flow, and the percentage increase in conductance. In six rat groups (half fasted and half with intrajejunal bile-oleate solutions), hyperemia parameters were determined before and after administration of either adenosine deaminase (ADA) or two adenosine receptor antagonists, namely 8-phenyltheophylline (8-PT) and 1,3-dipropyl-7-methylxanthine (DPMX). In fasted gut the three agents had variable effectiveness against reactive hyperemia, although 8-PT was the most consistent inhibitor. Instillation of intrajejunal lipid evoked a stable hyperemia and increased duration and blood flow volume after each occlusive period. ADA and 8-PT were more effective against reactive hyperemia in fed gut than in fasted gut. Our findings suggest that adenosine is a vasodilator metabolite modulating mesenteric reactive hyperemia, especially during enhanced intestinal metabolic activity.
Pawlik
W W
WW
Department of Medicine, University of Colorado School of Medicine, Denver.
Hottenstein
O D
OD
Palen
T E
TE
Pawlik
T
T
Jacobson
E D
ED
D000241:Adenosine / Q000037:antagonists & inhibitors / Q000494:pharmacology* / Q000502:physiology; D000243:Adenosine Deaminase / Q000494:pharmacology; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D006940:Hyperemia / Q000209:etiology / Q000503:physiopathology*; D007422:Intestines / Q000098:blood supply*; D008055:Lipids; D008297:Male; D008641:Mesenteric Vascular Occlusion / Q000503:physiopathology; D008823:Micelles; D058914:Purinergic Antagonists; D051381:Rats; D017207:Rats, Sprague-Dawley; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology
D008055:Lipids; D008823:Micelles; D058914:Purinergic Antagonists; D014970:Xanthines; C059953:1,3-dipropyl-7-methylxanthine; D013806:Theophylline; C028322:8-phenyltheophylline; D000243:Adenosine Deaminase; D000241:Adenosine