Immobilization of Schistosoma mansoni miracidia by activation of the alternate complement pathway at unusually high serum dilution.
Free-swimming Schistosoma mansoni miracidia were immobilized by adding normal mammalian serum to the water. Miracidial immobilizing activity (MIA) was shown to result from activating the alternate pathway of complement (APC). MIA in normal sera was heat-sensitive and antibody independent; it was greatly reduced in factor B-depleted or C6-depleted, but not in C1-depleted, human serum. Addition of purified factor B to B-depleted serum totally restored MIA. Half-maximal MIA in normal human, rabbit, and guinea pig sera was detectable at final dilutions exceeding 1/100, 1/200, and 1/500, respectively, and normal rat serum was particularly potent, with MIA at dilutions exceeding 1/2000. Detection of APC activity at such high dilutions is quite extraordinary and attributed to the hypotonic conditions. We confirmed and extended previous findings that heat-inactivated infection sera also display MIA. Immobilizing activity in irradiated-cercarial vaccine rat serum cofractionated with rat IgG and anti-SWAP antibody activity. Antibody-dependent MIA titres were much lower than for APC-dependent MIA. Based upon light microscope and transmission EM studies, immobilization of miracidia by APC activation was attributed to severe tegumental damage. Miracidia within egg shells were insensitive to MIA.
Knopf
P M
PM
Division of Biology & Medicine, Brown University, Providence, RI 02912.
Petzke
M M
MM
McLaren
D J
DJ
D000818:Animals; D003183:Complement C6 / Q000502:physiology; D011415:Complement Factor B / Q000502:physiology; D003170:Complement Pathway, Alternative / Q000502:physiology*; D006224:Cricetinae; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007814:Larva / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D009068:Movement; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012550:Schistosoma mansoni / Q000276:immunology* / Q000648:ultrastructure; D012555:Schistosomiasis mansoni / Q000276:immunology*
D003183:Complement C6; D007106:Immune Sera; D004533:Egtazic Acid; D004492:Edetic Acid; D011415:Complement Factor B