A novel pyrrolidonoalkaneamine (WR268954) that modulates chloroquine resistance of Plasmodium falciparum in vitro.
With the recent observations of efflux of chloroquine from Plasmodium falciparum and modulation of chloroquine resistance by calcium channel blockers, such as verapamil, a great deal of attention has been focused on the development of new modulators that can potentiate the efficacy of chloroquine. We report a new compound, WR268954, that has weak intrinsic antimalarial activity compared to chloroquine. In vitro, it increased the susceptibilities of chloroquine-resistant P. falciparum strains to chloroquine and quinine, but did not affect the chloroquine-susceptible strains. In the presence of 2,000 nM of WR268954, the 50% inhibitory concentration of chloroquine for drug-resistant P. falciparum decreased 90-fold in comparison with the control (chloroquine only). The same concentration of WR268954 increased the potentiation of chloroquine in resistant strains to a level approximately equivalent to that observed for the sensitive strain. This compound also potentiates the efficacy of quinine in drug-resistant parasites. However, WR268954 did not enhance the efficacy of mefloquine in the mefloquine-resistant parasites. In this report, the data show the synergistic effect of WR268954 on the antimalarial activity of chloroquine in drug-resistant strains of P. falciparum, but only an additive effect on drug-sensitive strains of parasites. Compound WR268954 belongs to a pyrrolidino alkane amine class whose in vitro chloroquine resistance modulator activity supports the basis for the synthesis of this class of compounds.
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Department of Parasitology, Walter Reed Army Institute of Research, Washington, District of Columbia.
Bhaduri
A P
AP
Milhous
W K
WK
D000818:Animals; D000840:Anisoles / Q000737:chemistry / Q000494:pharmacology*; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D002738:Chloroquine / Q000737:chemistry / Q000494:pharmacology*; D004351:Drug Resistance; D004357:Drug Synergism; D015394:Molecular Structure; D010963:Plasmodium falciparum / Q000187:drug effects*; D011759:Pyrrolidines / Q000737:chemistry / Q000494:pharmacology*; D011803:Quinine / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D000840:Anisoles; D000962:Antimalarials; D011759:Pyrrolidines; C082650:WR 268954; D002738:Chloroquine; D011803:Quinine; D014700:Verapamil