title: The in-vivo identification of the MoFe protein (FeMo cofactor) of nitrogenase in Klebsiella pneumoniae and of the Mo-storage protein in Azotobacter vinelandii via the nuclear quadrupole interaction of 99Mo(beta-)99Tc.
abstract: The expression of the MoFe protein of nitrogenase in Klebsiella pneumoniae was identified in vivo via the nuclear quadrupole interaction (NQI) of 99Mo(beta-)99Tc using perturbed angular correlations of gamma-rays. The NQI parameters were: omega approx. 360 Mrad/s and eta approx. 1. In addition, the NQI of the 'Mo-storage protein' in Azotobacter vinelandii cells which had been grown in the presence of NH4+ (13 mM), i.e. under conditions of strict repression of nitrogenase synthesis, was determined: omega approx. 190 Mrad/s, eta approx. 0.25. Under these conditions, the characteristic signal of the MoFe protein (FeMo cofactor) was absent.
authors.affiliation: Physik Department, Technische Universität München, Garching, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Mottner
authors.forename: T
authors.initials: T
authors.lastname: Butz
authors.forename: A
authors.initials: A
authors.lastname: Lerf
authors.forename: J
authors.initials: J
authors.lastname: Erfkamp
authors.forename: K
authors.initials: K
authors.lastname: Schneider
authors.forename: A
authors.initials: A
authors.lastname: Müller
mesh_terms: D016948:Azotobacter vinelandii / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008982:Molybdenum / Q000378:metabolism; D008983:Molybdoferredoxin / Q000032:analysis* / Q000302:isolation & purification; D009591:Nitrogenase / Q000737:chemistry*; D011854:Radiochemistry / Q000379:methods
chemical_list: D008983:Molybdoferredoxin; D008982:Molybdenum; D009591:Nitrogenase