Determination of linkage positions in peracetylated (methyl) xylo-oligosaccharides with fast atom bombardment mass spectrometry.
Distinction between the linkage types 1-->2, 1-->3 and 1-->4 of xylobioses can be achieved on the basis of the unimolecular decomposition spectra of the oxonium ions of the per-O-acetylated methyl glycosides. The spectra of the oxonium ions of various unbranched xylotri-, tetra- and pentaoses allow determination of the linkage position between the xylose residues. This indicates that in unbranched peracetylated xylo-oligosaccharides the linkage between the xylose residues at the non-reducing end can be determined.
Blok-Tip
L
L
Department of Mass Spectrometry, Bijvoet Center for Biomolecular Research, Universiteit Utrecht, The Netherlands.
van der Kerk-van Hoof
A
A
Heerma
W
W
Haverkamp
J
J
Kovácik
V
V
Hirsch
J
J
D002236:Carbohydrate Conformation; D009844:Oligosaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D009844:Oligosaccharides