Production of recombinant antibodies in lymphoid and non-lymphoid cells.
A recombinant tandem of 'chimeric' mouse/human immunoglobulin (Ig) genes was constructed and inserted into plasmid pGEM1 under the control of the T7 bacteriophage RNA polymerase promoter. Lymphoid (Sp2/0) and non-lymphoid (CHO) cell lines used for transfection contained in their genomes a semisynthetic gene of T7 RNA polymerase and steadily expressed this enzyme. It was shown for the first time that a stable polycystronic transcription of the Ig gene tandem occurs under the control of a single T7 phage promoter, both in lymphoid and non-lymphoid cells. Synthesis of kappa-light and epsilon-heavy Ig chains and functionally active antibodies was observed in the above-mentioned transfected cell lines.
Deyev
S M
SM
Laboratory of Molecular Genetic Immunology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences.
Lieber
A
A
Radko
B V
BV
Polanovsky
O L
OL
D000818:Animals; D000906:Antibodies / Q000235:genetics*; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006801:Humans; D051379:Mice; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014407:Tumor Cells, Cultured; D014764:Viral Proteins
D000906:Antibodies; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases