title: Primary structure of human milk octa-, dodeca- and tridecasaccharides determined by a combination of 1H-NMR spectroscopy and fast-atom-bombardment mass spectrometry. Evidence for a new core structure, the para-lacto-N-octaose.
abstract: Two octasaccharides, two dodecasaccharides and a tridecasaccharide have been isolated from human milk by a combination of paper chromatography, high-performance liquid chromatography and high-performance anion-exchange chromatography. Their structural analysis investigated by 400-MHz 1H- and 13C-NMR spectroscopy and by mass spectrometry led to the following structures. [formula: see text] The two octasaccharides have been previously characterized in human milk; the complete assignment of the 1H and 13C spectra is reported here. One of the dodecasaccharides is a tetrafucosyl derivative with a new core: typeI-(beta 1-3)-typeII-(beta 1-3)-typeII-(beta 1-3)-Gal((beta 1-4)Glc, where typeI = Gal(beta 1-3)GlcNAc and typeII = Gal(beta 1-4)GlcNAc. These oligosaccharides display simultaneously Lewis(b) and Lewis(x) determinants or Lewis(a), Lewis(b) and Lewis(x) determinants.
authors.affiliation: Laboratoire de Chimie Biologique, UMR du CNRS no. 111, Université des Sciences et Technologies de Lille, Villeneuve d'ascq, France.
authors.forename: S
authors.initials: S
authors.lastname: Haeuw-Fievre
authors.forename: J M
authors.initials: JM
authors.lastname: Wieruszeski
authors.forename: Y
authors.initials: Y
authors.lastname: Plancke
authors.forename: J C
authors.initials: JC
authors.lastname: Michalski
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
authors.forename: G
authors.initials: G
authors.lastname: Strecker
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D009682:Magnetic Resonance Spectroscopy; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D009844:Oligosaccharides