Improved chromatographic purification of pea seedlings diamine oxidase.
An improved and simplified purification procedure has been developed for the isolation of diamine oxidase from pea seedlings (DAO EC 1.4.3.6). It involves ammonium sulphate precipitation, hydrophobic interaction chromatography, ion-exchange chromatography and size-exclusion chromatography. The homogeneity of the final enzyme preparations and molecular weight were determined by size-exclusion chromatography and by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS PAGE). The isoelectric point of 7.35 +/- 0.05 was determined by chromatofocusing and by polyacrylamide gel isoelectric focusing.
Wimmerová
M
M
Department of Biochemistry, Masaryk University, Brno, Czechoslovakia.
Glatz
Z
Z
Janiczek
O
O
Macholán
L
L
D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000201:enzymology*; D007526:Isoelectric Point; D008970:Molecular Weight; D010946:Plants, Medicinal
D006631:Amine Oxidase (Copper-Containing)