Characterisation of Plesiomonas shigelloides strains that share type-specific antigen with Shigella flexneri 6 and common group 1 antigen with Shigella flexneri spp. and Shigella dysenteriae 1.
Three strains of Plesiomonas shigelloides isolated from patients with diarrhoea were agglutinated with Shigella flexneri 6 antiserum in slide and tube tests. All the strains were also agglutinated with a monoclonal antibody to the common group 1 antigen shared between S. flexneri serotypes and S. dysenteriae type 1. Further studies with one strain also showed sharing of antigenicity in an enzyme-linked immunosorbent assay. The results suggest that the strains share type-specific antigen with S. flexneri 6 and the common group 1 antigen with S. flexneri serotypes and S. dysenteriae 1. The sharing of antigens may have implications for cross-protection. One strain adhered to HEp-2 cell monolayers. None of the strains contained high mol. wt plasmids and there was no sequence homology with the invasiveness plasmid of Shigella spp. in DNA probe hybridisation. They were susceptible to the commonly used antibiotics. However, they possessed four other virulence-associated properties of Shigella spp. that included Congo-red binding, hydrophobicity, toxicity to HeLa cells and HEp-2 cell invasiveness (although they gave negative results in the Sereny test for invasiveness). These data suggest that the three unique strains might be considered pathogenic. Studies in animal models and human volunteers would be necessary to establish their pathogenic potential.
Albert
M J
MJ
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
Ansaruzzaman
M
M
Qadri
F
F
Hossain
A
A
Kibriya
A K
AK
Haider
K
K
Nahar
S
S
Faruque
S M
SM
Alam
A N
AN
D000372:Agglutination Tests; D000942:Antigens, Bacterial / Q000032:analysis*; D001422:Bacterial Adhesion; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D003603:Cytotoxins / Q000096:biosynthesis; D003967:Diarrhea / Q000382:microbiology*; D004405:Dysentery, Bacillary / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D016981:Plesiomonas / Q000276:immunology / Q000472:pathogenicity*; D012762:Shigella dysenteriae / Q000276:immunology / Q000472:pathogenicity*; D012763:Shigella flexneri / Q000276:immunology / Q000472:pathogenicity*; D014774:Virulence
D000942:Antigens, Bacterial; D003603:Cytotoxins