Studies on pharmacological activation of human serum immunoglobulin G by chemical modification and active subfragments. IX. Anti-inflammatory activity of completely alkylated heavy chain (C.Fr.I-H).
The anti-inflammatory activity of completely reduced and carboxamide-methylated heavy chain of all disulfide bonds in human serum immunoglobulin G (IgG) (C.Fr.I-H) was investigated using some experimental inflammatory models. C.Fr.I-H significantly inhibited carrageenin-induced paw edema and pleurisy, and serotonin-induced paw edema. The carboxamide-methylated heavy chain of interchain disulfide bonds in IgG (Fr.I-H) exhibited similar inhibitory activities to C.Fr.I-H on carrageenin-induced paw edema and pleurisy, while it was ineffective on serotonin-induced paw edema. These results suggest that complete alkylation of disulfide bonds in a heavy chain caused stronger anti-inflammatory activities than partial alkylation.
Mimura
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Itoh
M
M
Haruyama
M
M
Sugiyama
T
T
Suzuki
N
N
Tsujikawa
K
K
Kohama
Y
Y
D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan; D004220:Disulfides / Q000737:chemistry; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010446:Peptide Fragments / Q000494:pharmacology*; D010998:Pleurisy / Q000139:chemically induced / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012701:Serotonin
D000596:Amino Acids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004220:Disulfides; D007074:Immunoglobulin G; D010446:Peptide Fragments; D012701:Serotonin; D002351:Carrageenan