Differences in composition of cell-attachment sialoproteins between dentin and bone.
Matrices of dentin and bone were compared with respect to the content of cell-attachment sialoproteins. The levels of two sialoproteins, osteopontin (OPN) and bone sialoprotein (BSP), were determined in dentin and bone by immunochemical procedures. Polyclonal antibodies against bovine BSP and an antibody against the amino-terminal decapeptide of rat OPN were used. The relative levels of OPN and BSP in dentin were less than one-tenth of the levels in bone. The differences between dentin and bone levels of OPN and BSP were thus larger than those for osteonectin or bone Gla protein in the two tissues. The scarcity of the cell-attachment proteins in dentin may reflect the metabolic inactivity of dentin.
Fujisawa
R
R
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
Butler
W T
WT
Brunn
J C
JC
Zhou
H Y
HY
Kuboki
Y
Y
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003804:Dentin / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin / Q000032:analysis; D053495:Osteopontin; D010750:Phosphoproteins / Q000032:analysis*; D051381:Rats; D012795:Sialoglycoproteins / Q000032:analysis*; D060749:alpha-2-HS-Glycoprotein
D001798:Blood Proteins; D015815:Cell Adhesion Molecules; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; D010750:Phosphoproteins; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D060749:alpha-2-HS-Glycoprotein; C017630:phosphophoryn; D053495:Osteopontin