V region dependent selection of persistent resting peripheral B cells in normal mice.
In this study, we compare the VH gene family repertoire of persistent long-lived splenic B cells with that of the whole splenic B cell compartment. Persistent long-lived B cells were obtained by two independent strategies. Firstly, we studied cells that persist in the peripheral pools with or without cell division. We have transferred mature lipopolysaccharide (LPS)-reactive splenic B cells into LPS non-responder recipients and studied the donor population that could be recovered in host mice at differing time intervals after cell transfer. Secondly, we studied non-cycling resting long-lived B cells. These cells were obtained in normal mice after cytostatic treatment with hydroxyurea and in mice transgenic for the HSV-1 thymidine kinase gene after treatment with the anti-herpetic ganciclovir. Studying the VH gene family usage at the single level by in situ hybridization, we found that persistent B cells show an increased expression of the VHJ558 family and a diminished representation of the VH7183 family. These results demonstrate that in a normal non-immunized mouse, B cell survival and persistence in the resting state results from V region dependent selection.
Viale
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AC
Unité d'Immunobiologie, CNRS URA 359, Institut Pasteur, Paris, France.
Coutinho
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A
Heyman
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Freitas
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AA
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005803:Genes, Immunoglobulin; D006918:Hydroxyurea / Q000494:pharmacology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007399:Interphase; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D005810:Multigene Family
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D006918:Hydroxyurea