The complete amino acid sequence of a thrombin-like enzyme/gyroxin analogue from venom of the bushmaster snake (Lachesis muta muta).
The complete amino acid sequence of a thrombin-like enzyme with gyroxin activity isolated from the venom of the bushmaster snake Lachesis muta muta was determined by automated and DABITC/PITC microsequencing of the intact protein; fragments derived from it by separate cleavages with cyanogen bromide, iodosobenzoic acid and hydroxylamine; and peptides resulting from enzymatic digestions with trypsin, pepsin, chymotrypsin, and elastase. The protein, which is composed of 228 residues, contains four putative sites of N-linked glycosylation and exhibits significant sequence similarities with other serine proteases reported from snake venoms.
Magalhaes
A
A
Centro de Pesquisa e Desenvolvimento, Fundacão Ezequiel Dias, Belo Horizonte (MG), Brazil.
Da Fonseca
B C
BC
Diniz
C R
CR
Gilroy
J
J
Richardson
M
M
D000595:Amino Acid Sequence; D001665:Binding Sites; D002918:Chymotrypsin / Q000378:metabolism; D003435:Crotalid Venoms / Q000737:chemistry* / Q000378:metabolism; D003488:Cyanogen Bromide; D006031:Glycosylation; D019811:Hydroxylamine; D006898:Hydroxylamines; D007463:Iodobenzoates; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry* / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D003435:Crotalid Venoms; D006898:Hydroxylamines; D007463:Iodobenzoates; C072644:Lachesis venom; D010446:Peptide Fragments; D019811:Hydroxylamine; C008154:2-iodosobenzoic acid; C017356:gyroxin; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; D013917:Thrombin; D010434:Pepsin A; D003488:Cyanogen Bromide