Pharmacokinetics and biodistribution of radiolabeled avidin, streptavidin and biotin.
The extraordinary high affinity of avidin and streptavidin for biotin may be exploited in a two-step approach for delivering radiolabeled biotin derivatives suitable for imaging and therapy to target-bound streptavidin or avidin conjugated monoclonal antibodies (MAbs). The in vivo pharmacokinetics and biodistribution of radiolabeled avidin, streptavidin (SA) and DTPA-biocytinamide (DTPA-biotin) were studied in the rabbit and dog. SA circulated in the blood similar to other 60 kDa proteins, avidin cleared immediately and DTPA-biotin exhibited plasma clearance by glomerular filtration.
Rosebrough
S F
SF
Department of Radiology, University of Rochester Medical Center, NY 14642.
D000818:Animals; D001360:Avidin / Q000493:pharmacokinetics*; D001426:Bacterial Proteins / Q000493:pharmacokinetics*; D001710:Biotin / Q000737:chemistry / Q000493:pharmacokinetics*; D004285:Dogs; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D004369:Pentetic Acid / Q000737:chemistry / Q000493:pharmacokinetics; D011817:Rabbits; D019809:Streptavidin; D014018:Tissue Distribution
D001426:Bacterial Proteins; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D001360:Avidin; D001710:Biotin; D004369:Pentetic Acid; D019809:Streptavidin