Bispecific monoclonal antibodies to human interleukin 2 and horseradish peroxidase.
Hybrid hybridomas (tetradomas), producing bispecific monoclonal antibodies (bmabs) binding to both interleukin 2 and horseradish peroxidase were obtained by fusing IL 2-specific and HRP-specific hybridomas. Parental hybridomas were labelled prior to the fusion with fluorescein isothiocyanate and tetramethylrhodamine isothiocyanate. Bifluorescent (tetradoma) cells were sorted out using fluorescence activated cell sorter. Two clones, designated D8C1/G and H7C11/H were shown to secrete bmabs over at least 6 months growth in culture. Bmabs have been purified from mouse ascites by ammonium sulfate precipitation and affinity chromatography on immobilized peptide, modelling corresponding IL 2 epitope. The purity of antibodies obtained was characterized by capillary electrophoresis. The possibility of using these antibody preparations in rIL 2 analysis was evaluated in two types of EIA: direct and competitive solid-phase EIA. Both assays had similar sensitivity of about 1.5-4 ng IL 2 per ml.
Stratieva-Taneeva
P A
PA
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Khaidukov
S V
SV
Kovalenko
V A
VA
Nazimov
I V
IV
Samokhvalova
L V
LV
Nesmeyanov
V A
VA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002459:Cell Fusion; D002469:Cell Separation; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology*; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D007376:Interleukin-2; D011994:Recombinant Proteins; D006735:Horseradish Peroxidase