Effects of hyaluronate, strontium and prolonged incubation on different sperm parameters.
The aim of this study was to evaluate the effect of hyaluronate and strontium with short and prolonged incubation periods on different sperm parameters. For sperm intended for in vitro fertilization good progressive motility, a high percentage of normal form, a suitable linear velocity and a low acrosome-reacted proportion of sperm are required. The semen washing procedure in the self-migration method with medium containing hyaluronate Ca2+ after 3 h of incubation complies better with these criteria.
Psalti
I
I
Physiology of Human Reproduction Research Unit, University of Louvain, Brussels, Belgium.
Thomas
K
K
De Cooman
S
S
D000177:Acrosome / Q000187:drug effects; D002122:Calcium Chloride / Q000494:pharmacology; D003470:Culture Media; D005307:Fertilization in Vitro; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013324:Strontium / Q000494:pharmacology*; D013997:Time Factors
D003470:Culture Media; D006820:Hyaluronic Acid; C025700:strontium chloride; D002122:Calcium Chloride; D013324:Strontium