The upregulation of a glial cell surface antigen at the astrocytic scar in the rat.
The upregulation of a 106-kDa glial protein was examined using indirect immunohistochemical methods. The protein is recognized by the monoclonal antibody AMP1. Previous studies demonstrated that the AMP1 antigen is found on the external surface of cultured astrocytes and is involved in stabilizing adhesive interactions between these cells. In sections of injured adult rat brain, the spatial distribution of the AMP1 antigen correlated with the region of reactive gliosis. The relative intensity of immunofluorescence indicates that the AMP1 antigen is dramatically upregulated at the gliotic scar. The correlation between AMP1 antigen upregulation and reactive gliosis suggests that this molecule is critical to the process of CNS scar formation.
Irwin
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MH
Department of Cell Biology, University of Alabama Medical Center, Birmingham 35294-0019.
Geisert
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EE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D001253:Astrocytes / Q000276:immunology*; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009457:Neuroglia / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015816:Cell Adhesion Molecules, Neuronal; D005904:Glial Fibrillary Acidic Protein