Quantitation of HTLV-I provirus among seropositive blood donors: relation with antibody profile using synthetic peptides.
To study the pathophysiological state of HTLV-I carriers and the quantitative aspect of the risk of HTLV-I infection, we measured the absolute amounts of HTLV-I provirus in 39 seropositive blood donors. The amount of provirus varied from less than one to more than 10(4) molecules per 10(5) peripheral-blood mononuclear cells (PBMC). The average amount of HTLV-I provirus among seropositive blood donors was estimated as 3.7 x 10(3) molecules per 10(5) PBMC. The titer of the antibody against only the synthetic peptide (aa 100 to 130) of the gag p19 region of the HTLV-I provirus showed a weak but significant correlation with the amount of the provirus.
Matsumura
M
M
Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
Kushida
S
S
Ami
Y
Y
Suga
T
T
Uchida
K
K
Kameyama
T
T
Terano
A
A
Inoue
Y
Y
Shiraki
H
H
Okochi
K
K
D000328:Adult; D000595:Amino Acid Sequence; D001782:Blood Donors; D002353:Carrier State / Q000276:immunology / Q000382:microbiology; D005260:Female; D015481:HTLV-I Antibodies / Q000097:blood*; D015490:HTLV-I Infections / Q000276:immunology / Q000382:microbiology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011533:Proviruses / Q000276:immunology / Q000302:isolation & purification*
D015481:HTLV-I Antibodies