Construction of new T vectors for direct cloning of PCR products.
More than half of the products of PCR contain an extra A residue at the 3' end, which is the result of the template-independent activity of Taq polymerase. To facilitate cloning of the products of PCR without modification, T vectors, which have a single overhanging T residue at the 3' end, have been developed. In the present study, we constructed new T vectors which can be prepared in the laboratory by simple digestion with the restriction enzymes AspEI or Eam1 105I.
Ichihara
Y
Y
Institute for Comprehensive Medical Science, Fujita Health University, Aichi, Japan.
Kurosawa
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D015252:Deoxyribonucleases, Type II Site-Specific; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D013941:Thymine
C075914:endodeoxyribonuclease Eam1105I; D015252:Deoxyribonucleases, Type II Site-Specific; D013941:Thymine