Purification and properties of selenoprotein W from rat muscle.
Following injection with [75Se]selenite, a low molecular weight 75Se-selenocysteine containing protein was purified from rat muscle. The purification procedure involved ammonium sulfate fractionation, Sephadex G-50 gel filtration, cation exchange chromatography on CM-Sephadex, and reverse phase high pressure liquid chromatography using a C-18 Vydac column. Four forms of the protein were separated by the cation exchange and reverse phase chromatography steps. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of the four proteins revealed masses of 9550 +/- 1, 9596 +/- 1.2, 9858 +/- 1.3, and 9898 +/- 1.1 daltons. Glutamate, glycine, lysine, leucine, and valine are the major amino acids in this protein. About 0.92 g atoms of selenium was found per g mol of protein, and this selenium was present as selenocysteine. Thus, this appears to be a new selenoprotein, and we have named it selenoprotein W.
Vendeland
S C
SC
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
Beilstein
M A
MA
Chen
C L
CL
Jensen
O N
ON
Barofsky
E
E
Whanger
P D
PD
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008297:Male; D013058:Mass Spectrometry; D008970:Molecular Weight; D009132:Muscles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000302:isolation & purification / Q000378:metabolism*; D051151:Selenoprotein W; D051140:Selenoproteins
D000596:Amino Acids; D011506:Proteins; D051151:Selenoprotein W; D051140:Selenoproteins; D012643:Selenium