Divergence of two families of repeated DNA in wood and field mice of the genus Apodemus (Muridae, Rodentia).
The nDNA of six species of a wood and field mice of the genus Apodemus (2n = 48): peninsulae, sylvaticus, flavicollis, microps, speciosus, agrarius, grey rat Rattus norvegicus as well, and house mouse Mus musculus were studied using EcoRI and HindIII restriction endonucleases with an agarose and polyacrylamide gel electrophoresis and a microdensitometry. It is shown for dispersed sequences that a quarter of 1.85 kb EcoRI genus specific family of repeated DNA is constant but a quarter of 1.35 kb EcoRI repeats, which are specific for more large taxon unit, is variable. The HindIII satDNA of the various species differs in the length of monomer, size of more representative multimer and digestion of the monomers by the same endonuclease of restriction; HindIII satDNA quantity correlates with 1.35 kb EcoRI repeat content. Some nonidentified components are found in Apodemus genomes. The possible ways evaluation of the repetitive DNA are briefly discussed. It was supposed that the evolutionary age of the wood mice is directly proportional to the length of HindIII multimer and inversely proportional to the amount of HIndIII satDNA with a 1.35 kb EcoRI repeats.
Chelomina
G N
GN
D000818:Animals; D005075:Biological Evolution; D004247:DNA / Q000235:genetics*; D016678:Genome; D051379:Mice; D009115:Muridae / Q000235:genetics*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004247:DNA
Divergentsiia dvukh semeĭstv povtoriaiushcheĭsia DNK lesnykh i polevykh mysheĭ roda APodemus (Muridae, Rodentia).