Apoptosis induction in 32D cells by IL-3 withdrawal is preceded by a drop in the intracellular calcium level.
The 32D murine myeloid cell line is dependent on interleukin-3 (IL-3) for growth in vitro. We show here that IL-3 induces a slow increase in endocellular calcium, which is sizeable two hours after the addition. Withdrawal of the cytokine induces apoptosis, whose classic late events are evident after 16/18 hours, and are preceded by a calcium drop during the first 2/3 hours after IL-3 subtraction. Calcium drop is here proposed as a trigger of the apoptotic process, in agreement with other recently reported findings.
Magnelli
L
L
Laboratory of Molecular Biology, Institute of General Pathology, Firenze, Italy.
Cinelli
M
M
Turchetti
A
A
Chiarugi
V P
VP
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D007377:Interleukin-3 / Q000172:deficiency*; D051379:Mice
D007377:Interleukin-3; D002118:Calcium