title: Immune response against Trypanosoma cruzi antigens in Cebus apella monkeys.
abstract: The american primate Cebus apella has been used as an experimental model for the study of acute and chronic Chagas' disease. The antibody response elicited by 4 x 10(6) blood trypnomastigotes injected into four monkeys was analysed. Peak titres of IgM and IgG of anti-Trypanosoma cruzi antibodies were found at day 22, and between days 20 and 40 post-infection (p.i.), respectively. The ability of a Mr 37kDa (T37K) glycoprotein purified from T. cruzi epimastigotes to generate IgG anti-T. cruzi antibodies in monkeys, and protect them against a challenge with trypomastigotes, was also studied. Monkeys non-immunized with T37K reached peak values of parasitaemia between days 18 and 21 post-infection, whereas immunized monkeys had lower parasitaemias without important variation. Anti-T37K antibodies in immunized monkeys decreased from day 2 with the lowest titres between days 14 to 22 p.i., coincident with the peak of parasitaemia in control non-immunized monkeys. These results suggest that anti-T37K antibodies could be responsible for the low parasitaemia detected in immunized monkeys.
authors.affiliation: Departamento de Microbiologia, Immunologia y Biotecnologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.
authors.forename: E L
authors.initials: EL
authors.lastname: Malchiodi
authors.forename: C H
authors.initials: CH
authors.lastname: Carbonetto
authors.forename: D
authors.initials: D
authors.lastname: Grana
authors.forename: K
authors.initials: K
authors.lastname: Eiguchi de Palmero
authors.forename: M G
authors.initials: MG
authors.lastname: Chiaramonte
authors.forename: C A
authors.initials: CA
authors.lastname: Falasca
authors.forename: R A
authors.initials: RA
authors.lastname: Margni
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D002428:Cebus; D014355:Chagas Disease / Q000276:immunology* / Q000517:prevention & control; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008297:Male; D016052:Protozoan Vaccines / Q000276:immunology; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000276:immunology*; D014611:Vaccination
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D016052:Protozoan Vaccines