Molecular cloning of two additional members of the neural visinin-like Ca(2+)-binding protein gene family.
We have isolated a rat cDNA clone encoding a neural visinin-like Ca(2+)-binding protein (NVP), which we designate NVP-1. To identify additional molecular forms of NVP, a rat brain cDNA library was screened for their presence using an NVP-1 cDNA probe under low-stringency hybridization conditions. Two types of cDNA clones encoding structurally related proteins, designated NVP-2 and NVP-3, have been isolated. The deduced amino acid sequences of NVP-2 and NVP-3 are 89.0% and 68.6% identical to that of NVP-1, respectively, and contain consensus sequences for EF-hand Ca(2+)-binding sites. Northern blot analysis shows that NVP-1, NVP-2, and NVP-3 mRNAs are most highly expressed in brain and are differentially expressed in various regions of rat brain. These results suggest that NVP-2 and NVP-3 are additional members of the NVP gene family.
Kajimoto
Y
Y
Department of Pharmacology, Kobe University School of Medicine, Japan.
Shirai
Y
Y
Mukai
H
H
Kuno
T
T
Tanaka
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005796:Genes; D007536:Isomerism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics*; D051596:Neurocalcin; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D044169:Receptors, Calcium-Sensing; D017385:Sequence Homology; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D012333:RNA, Messenger; D044169:Receptors, Calcium-Sensing; C496690:Vsnl1 protein, rat; D004247:DNA