Demonstration of a human urinary trypsin inhibitor (urinastatin)-like substance in the murine brain.
A human urinary trypsin inhibitor (urinastatin)-like immunoreactive substance with trypsin-inhibitory activities was found in the murine brain. Regional levels of this urinastatin (UT)-like substance in the brain were within 5 ng/mg protein and were expressed in a descending order as follows: cerebral cortex not equal to hippocampus > hypothalamus > mesencephalon not equal to corpus striatum >> medulla oblongata > cerebellum. Mechanical lesioning of the cerebral cortex or hippocampus with a needle induced a rapid and intense appearance of a UT-like immunoreactive substance in the neuronal cells of injured sites. Conditioned fear-stress induction incited a reversible increase in the level of UT-like immunoreactive substance in the hippocampus. These results suggest that the UT-like immunoreactive substance is produced by neurons in response to brain injury and fear-stress stimuli.
Shikimi
T
T
Department of Pharmacology, Shimane Medical University, Japan.
Wessel
T
T
Joh
T H
TH
Takahashi
M
M
Kaneto
H
H
Hattori
K
K
Takaori
S
S
D001921:Brain / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005239:Fear; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D013315:Stress, Psychological / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000032:analysis / Q000378:metabolism*
D006023:Glycoproteins; D014361:Trypsin Inhibitors; C028665:urinastatin