Detection of tetracycline resistance protein encoded by Bacillus subtilis plasmid pNS1.
Tetracycline resistance protein (TET) of Bacillus subtilis plasmid pNS1 was detected by immunoblot analysis using a specific antibody to TET-chloramphenicol acetyltransferase (CAT) fusion protein. In two-dimensional electrophoresis, one major spot which seemed to be the pNS1-encoded TET (pNS1-TET), was detected by immunostaining. Its molecular weight and isoelectric point were approximately 52 kDa and 6.2, respectively. Judging from the nucleotide sequence, the pNS1-TET is a very hydrophobic, 50 kDa protein. Therefore, the 52 kDa protein is thought to be an intact form of the pNS1-TET produced by B. subtilis cells.
Aoki
T
T
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University, Hokkaido, Japan.
Kaneta
M
M
Watabe
H
H
D000911:Antibodies, Monoclonal; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007526:Isoelectric Point; D008970:Molecular Weight; D011815:R Factors; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology; D012097:Repressor Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D013753:Tetracycline Resistance
D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein; D015500:Chloramphenicol O-Acetyltransferase