Antibody responses of children to the C-terminal peptide of the SH protein of respiratory syncytial virus and the immunological characterization of this protein.
The SH protein of RSV, a small integrated hydrophobic membrane protein, consists of 64 amino acid residues in the polypeptide of subgroup A and 65 amino acid residues in the polypeptide of subgroup B. We synthesized five peptides, representing the SH protein of each RSV subgroup comprised of the following amino acid residues: 2-16, 12-26, 35-49, 45-60, and for subgroup A, 51-64 and for subgroup B, 51-65. Peptides 2-16 and 51-64/65 represented the N-terminal and C-terminal ends of the protein, respectively. In RIPA, under reducing conditions with mercaptoethanol, hyperimmune guinea pig (GP) serum against C-terminal peptide of the two subgroups precipitated the homologous 7.5 kDa and 21-30 kDa SH proteins. Under nonreducing conditions, the GP antipeptide sera precipitated all three SH proteins, suggesting that the 13-15 kDa protein exists as a dimer. The subgroup A 7.5 and 13-15 kDa proteins had apparent molecular weights about 1-2 kDa higher than the corresponding subgroup B proteins. The C-terminal peptides of subgroups A and B were used to characterize the immune response of 11 children, age 1 month to 1 year, with presumed primary RSV infection. Three of 4 children with subgroup A infection and 4 of 7 children with subgroup B infection developed homologous 4-fold rises in antibody to C-terminal peptide (aa 51-64/65) during convalescence. Except for one child with subgroup A and one child with subgroup B infection, the other 5 children developed heterologous rises also.(ABSTRACT TRUNCATED AT 250 WORDS)
Akerlind-Stopner
B
B
Department of Virology, Karolinska Institute, School of Medicine, Stockholm, Sweden.
Hu
A
A
Mufson
M A
MA
Utter
G
G
Norrby
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002213:Capsid / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D015707:HN Protein; D006801:Humans; D007106:Immune Sera; D007223:Infant; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology*; D011233:Precipitin Tests; D012136:Respiratory Syncytial Viruses / Q000737:chemistry / Q000276:immunology*; D010253:Respirovirus Infections / Q000276:immunology*; D012680:Sensitivity and Specificity; D014709:Vero Cells; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D015707:HN Protein; D007106:Immune Sera; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G