title: Heterogeneity in the IgE binding to a peptide derived from the house dust mite allergen Der p II indicates that the IgE response is highly polyclonal.
abstract: The fine specificity of IgE antibody binding to peptide 65-78 of the house dust mite major allergen Der p II was examined by comparison with binding to two peptides in which the cysteines corresponding to cys73 and cys78 in Der p II were substituted by serines and methionines. Differences in binding behavior indicated that at least three different subpopulations of IgE antibodies bound to peptide 65-78. Even at the level of such a small fragment the IgE response in individual donors proved to be polyclonal.
authors.affiliation: Central Laboratory of The Netherlands, Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: W
authors.initials: W
authors.lastname: van't Hof
authors.forename: M
authors.initials: M
authors.lastname: van den Berg
authors.forename: P C
authors.initials: PC
authors.lastname: Driedijk
authors.forename: R C
authors.initials: RC
authors.lastname: Aalberse
mesh_terms: D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D039741:Antigens, Dermatophagoides; D001666:Binding Sites, Antibody / Q000187:drug effects; D006023:Glycoproteins / Q000737:chemistry* / Q000494:pharmacology; D007073:Immunoglobulin E / Q000378:metabolism*; D008925:Mites / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011485:Protein Binding
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D010446:Peptide Fragments; D007073:Immunoglobulin E