Stimulation of protein kinase C by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rat thymocytes.
Exposure of immature thymocytes to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (10 nM) transiently increased detergent extractable protein kinase C (PKC) activity. The increase was apparent at 7.5 min after exposure of cells to TCDD. At later time points, PKC activity returned to control values. Addition of TCDD to cytosol significantly increased phospholipid-stimulated PKC activity. Thus, stimulation of PKC activity may be one of the early events affected by TCDD.
DePetrillo
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PB
Program in Clinical Pharmacology, Brown University School of Medicine, Rhode Island Hospital (SWP-406), Providence 02903.
Kurl
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D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D008297:Male; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013950:Thymus Gland / Q000187:drug effects* / Q000201:enzymology*
D000072317:Polychlorinated Dibenzodioxins; D011493:Protein Kinase C