title: Similarity in nucleotide sequence of the gene encoding nontoxic component of botulinum toxin produced by toxigenic Clostridium butyricum strain BL6340 and Clostridium botulinum type E strain Mashike.
abstract: The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb HindIII fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C. butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component.
authors.affiliation: Department of Microbiology, School of Medicine, Sapporo Medical University, Hokkaido, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Yokosawa
authors.forename: K
authors.initials: K
authors.lastname: Oguma
authors.forename: T
authors.initials: T
authors.lastname: Yashiki
authors.forename: K
authors.initials: K
authors.lastname: Takeshi
authors.forename: T
authors.initials: T
authors.lastname: Ohyama
authors.forename: E
authors.initials: E
authors.lastname: Isogai
authors.forename: H
authors.initials: H
authors.lastname: Isogai
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D003013:Clostridium / Q000145:classification / Q000235:genetics*; D003014:Clostridium botulinum / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D001905:Botulinum Toxins