Immunoperoxidase staining of non-Hodgkin's lymphomas for T-cell lineage associated antigens in paraffin sections. Comparison of the performance characteristics of four commercially available antibody preparations.
Paraffin sections of 133 cases of non-Hodgkin's lymphoma (69 B-cell type and 64 T-cell type) were stained in a labeled streptavidin biotin immunoperoxidase technique with a panel of antibodies that recognized T-lymphocyte associated antigens. This study was done to determine the sensitivity and specificity of these reagents for phenotyping T-cell lymphomas. UCHL-1, polyclonal anti-CD3, Leu-22, and OPD4 stained 78%, 72%, 91%, and 69% of the cases of T-cell lymphomas, respectively. The phenotype of L-26 negative and CD3 or UCHL-1 positive accurately predicted T-cell phenotype in 95% (60 of 63) of the T-cell lymphomas and was not seen in any of the cases of B-cell lymphoma. Although Leu-22 was the most sensitive T-cell-associated marker in this series, its lack of specificity for T-lymphocytes limited its usefulness as part of a routine panel designed to distinguish between T-cell and B-cell lymphomas. In conjunction with other reports, this study supports the use of the T-cell markers CD3 and UCHL-1 in combination with the B-cell-associated marker L-26 to phenotype most efficiently non-Hodgkin's lymphomas in paraffin sections.
Kurtin
P J
PJ
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905.
Roche
P C
PC
D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping / Q000379:methods; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000473:pathology*; D016612:Paraffin Embedding
D015703:Antigens, CD; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte