Paired STSs amplified from radiation hybrids, and from associated YACs, identify highly polymorphic loci flanking the ataxia telangiectasia locus on chromosome 11q22-23.
The high resolution mapping of the ataxia telangiectasia (A-T) locus on chromosome 11q22-23 requires the generation of new polymorphic markers specifically within the segment of 11q22-23 to which the locus has been assigned. We have made use of a library of Alu-PCR clones, amplified from a radiation reduced somatic cell hybrid containing the relevant chromosome 11 segment, to generate sequence tagged sites (STS) within the 11q22-23 region and have used YAC clones to extend the loci identified by these STSs. The identification of paired polymorphisms (from Alu-PCR and the associated YAC derived clone), which are physically linked, but which show minimal linkage disequilibrium, provides a highly informative haplotype for use in genetic linkage analysis in A-T families. We describe the characterisation of 2 such polymorphic loci, D11S535 and D11S611, which map between existing flanking markers, and which provide additional information on the location of the major A-T locus.
McConville
C M
CM
Department of Cancer Studies, Medical School, University of Birmingham, UK.
Byrd
P J
PJ
Ambrose
H J
HJ
Stankovic
T
T
Ziv
Y
Y
Bar-Shira
A
A
Vanagaite
L
L
Rotman
G
G
Shiloh
Y
Y
Gillett
G T
GT
D001260:Ataxia Telangiectasia / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D002880:Chromosomes, Human, Pair 11; D004247:DNA / Q000235:genetics; D005260:Female; D015723:Gene Library; D005819:Genetic Markers; D015894:Genome, Human; D006239:Haplotypes; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites
D005819:Genetic Markers; D004247:DNA