[The effect of nitroglycerin on the Ca2+ ion level in the cytoplasm of human lymphocytes].
Fluorescence was used to examine the effects of nitroglycerin (60 microM) on basal and mitogen-induced (ConA, 25 micrograms/ml) Ca2+ levels in the human lymphocytic cytoplasm. Calcium-blocking effect of nitroglycerin was markedly shown in the medium containing 1 mM CaCl2 and virtually absent in calcium-free buffer. It is concluded that nitroglycerin affects cellular Ca2+ entry. A 120-minute preincubation led to a complete loss of the inhibitory effects of the drug on mitogen-induced Ca2+ responses of lymphocytes. Human lymphocytes are proposed as an experimental model for investigating the effects of nitrates and the mechanisms of their tolerance development.
Sergeev
P V
PV
Storozhakov
G I
GI
Khamitova
E S
ES
Dukhanin
A S
AS
Shilo
V Iu
VIu
Trepilets
V E
VE
D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002478:Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D003593:Cytoplasm / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D004361:Drug Tolerance; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D005996:Nitroglycerin / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
D003208:Concanavalin A; D005996:Nitroglycerin; D002118:Calcium
Vliianie nitroglitserina na soderzhanie ionov Ca2+ v tsitoplazme limfotsitov cheloveka.