title: Co-operation of interleukin-1 beta and tumour necrosis factor-alpha in the activation of human umbilical vein endothelial cells to inhibit Toxoplasma gondii replication.
abstract: The tachyzoites of the RH strain of Toxoplasma gondii invaded and replicated in cultured human umbilical vein endothelial cells (HUVEC). Co-treatment of the HUVEC with human recombinant interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha) inhibited the replication of T. gondii. Growth of the parasite was measured in vitro by [3H]uracil incorporation assay 18 hr after infection. This assay showed that when cells were pretreated with IL-1 beta and TNF-alpha concentrations ranging from 1 to 100 U/ml, a high degree of inhibition was observed with a phenomenon of co-operation causing a dose-dependent inhibition of the replication of intracellular T. gondii. The mechanism(s) by which the inhibition of multiplication was effected remains to be elucidated.
authors.affiliation: Unité de Recherche Université-INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques de Tours, Nouzilly, France.
authors.forename: I H
authors.initials: IH
authors.lastname: Dimier
authors.forename: D T
authors.initials: DT
authors.lastname: Bout
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D004730:Endothelium, Vascular / Q000276:immunology*; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D014122:Toxoplasma / Q000254:growth & development*; D014123:Toxoplasmosis / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*; D014471:Umbilical Veins / Q000276:immunology*
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha