Production of anti-cardiolipin antibody in AKR/J mice with streptozocin-induced insulitis and diabetes.
We herein report that anti-cardiolipin antibodies (ACA) were detected in AKR/J mice treated with multiple low doses of streptozocin (STZ)-induced insulitis and diabetes. Daily intraperitoneal (i.p.) injections of 40 mg/kg body wt. of STZ for five consecutive days in the AKR/J mice resulted in hyperglycemia and mononuclear cell infiltrations of islets (insulitis). ACA appeared on day 14, when hyperglycemia began to occur, at a rate of 13.3% (4/30). The rate increased to 83.3% (25/30) on day 21, when diabetes developed, and then fell to 10% (3/30) on day 28. Neither the diabetic AKR/J mice treated with a single high dose of STZ (200 mg/kg body wt.) nor the non-diabetic insulitis free Balb/c mice and B10.S(9R) mice treated with multiple low doses of STZ (40 mg/kg body wt.) produced ACA. The IgG subclass of the ACA belonged mainly to IgG2a. These findings suggest that ACA are produced in association with the development of insulitis, but not induced by either hyperglycemia or STZ.
Anzai
K
K
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Nakamura
M
M
Nagafuchi
S
S
Iwakiri
R
R
Ichinose
I
I
Mitsugi
K
K
Kuroki
M
M
Nakano
S
S
Niho
Y
Y
D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000096:biosynthesis / Q000097:blood*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000276:immunology* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D006943:Hyperglycemia / Q000097:blood; D007515:Islets of Langerhans / Q000473:pathology; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D010182:Pancreatic Diseases / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D010743:Phospholipids / Q000378:metabolism
D017153:Antibodies, Anticardiolipin; D010743:Phospholipids