title: Successful primate immunization with peptides conjugated to purified protein derivative or mycobacterial heat shock proteins in the absence of adjuvants.
abstract: We have previously shown in mice that antibodies can be induced to synthetic malaria peptides conjugated to mycobacterial antigens, such as purified protein derivative (PPD) or heat shock proteins (hsp), and given in the absence of adjuvants after a previous priming with bacille Calmette-Guérin (BCG). In the present study we investigated this model of immunization in the non-human primates, Saimiri sciureus monkeys. Monkeys primed with BCG subcutaneously and then immunized subcutaneously with the Plasmodium falciparum sporozoite (NANP)40 synthetic peptide conjugated to PPD or mycobacterial hsp of 65 or 70 kD, in the absence of adjuvants, produced antipeptide and anti-sporozoite IgG antibodies. Interestingly, the carrier effect of the hsp of 70 kD for the induction of anti-(NANP)40 antibodies was also observed in the absence of a previous priming with BCG. These data suggest that such a vaccination strategy may be applied to humans.
authors.affiliation: World Health Organization-Immunology Research and Training Centre, Department of Pathology, University of Geneva, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Perraut
authors.forename: A R
authors.initials: AR
authors.lastname: Lussow
authors.forename: S
authors.initials: S
authors.lastname: Gavoille
authors.forename: O
authors.initials: O
authors.lastname: Garraud
authors.forename: H
authors.initials: H
authors.lastname: Matile
authors.forename: C
authors.initials: C
authors.lastname: Tougne
authors.forename: J
authors.initials: J
authors.lastname: van Embden
authors.forename: R
authors.initials: R
authors.lastname: van der Zee
authors.forename: P H
authors.initials: PH
authors.lastname: Lambert
authors.forename: J
authors.initials: J
authors.lastname: Gysin
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001500:BCG Vaccine / Q000276:immunology; D005260:Female; D006360:Heat-Shock Proteins / Q000008:administration & dosage* / Q000276:immunology; D007114:Immunization; D008297:Male; D009161:Mycobacterium / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D012453:Saimiri; D014373:Tuberculin / Q000008:administration & dosage* / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D001500:BCG Vaccine; D006360:Heat-Shock Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014373:Tuberculin; D014614:Vaccines, Synthetic; C041595:circumsporozoite protein, Protozoan