Correction of lysosomal storage in the liver and spleen of MPS VII mice by implantation of genetically modified skin fibroblasts.
Genetic defects of lysosomal hydrolases result in severe storage diseases and treatments based on enzyme replacement have been proposed. In mice lacking beta-glucuronidase, which develop a disease homologous to human mucopolysaccharidosis type VII (Sly syndrome), we have used autologous implants of genetically-modified skin fibroblasts for the continuous in vivo production of the enzyme. The human beta-glucuronidase cDNA was introduced with a retroviral vector into mutant mice skin fibroblasts grown in primary culture. Fourteen mutant mice were implanted intraperitoneally with these modified cells embedded into collagen lattices. All animals expressed beta-glucuronidase from the vascularized neo-organs that developed after implantation and accumulated the enzyme in their tissues. A complete disappearance of the lysosomal storage lesions was observed in their liver and spleen.
Moullier
P
P
Laboratoire Rétrovirus et Transfert Génétique, Institut Pasteur, Paris, France.
Bohl
D
D
Heard
J M
JM
Danos
O
O
D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000201:enzymology / Q000637:transplantation*; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D005966:Glucuronidase / Q000008:administration & dosage / Q000172:deficiency* / Q000235:genetics / Q000627:therapeutic use; D006025:Glycosaminoglycans / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008247:Lysosomes / Q000201:enzymology*; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*; D016538:Mucopolysaccharidosis VII / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D009928:Organ Specificity; D010529:Peritoneal Cavity; D010641:Phenotype; D019736:Prostheses and Implants; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D012190:Retroviridae / Q000235:genetics; D013154:Spleen / Q000201:enzymology* / Q000473:pathology; D014162:Transfection
D006025:Glycosaminoglycans; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase