Cholesterol side-chain cleavage by immobilized cells of Rhodococcus equi DSM 89-133.
Side-chain cleavage of sterol and extracellular cholesterol oxidase activity were investigated using viable cells of R. equi DSM 89-133 immobilized in polyacrylamide gel. In batch culture, immobilized cells were active in side-chain cleavage of cholesterol for more than 30 days. Free or immobilized cells were incapable of side-chain cleavage in the absence of 2,2' dipyridyl; cholesterol oxidase was, however, produced in both the cases. Maximal activity of the immobilized cells was 60 to 70% of the free cells.
Ahmad
S
S
Department of Microbiology, College of Basic Sciences and Humanities, G B Pant University of Agriculture and Technology, Pantnagar, India.
Roy
P K
PK
Basu
S K
SK
Johri
B N
BN
D015082:2,2'-Dipyridyl / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D016929:Rhodococcus equi / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D015082:2,2'-Dipyridyl; D002784:Cholesterol; D002789:Cholesterol Oxidase