Characterization of two acidic polysaccharides having immunological activities from the root of Panax ginseng.
Two acidic polysaccharides, named ginsenan PA and ginsenan PB, were isolated from the root of Panax ginseng C. A. Meyer. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 1.6 x 10(5) and 5.5 x 10(4), respectively. They are composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid: D-glucuronic acid in the molar ratios of 11:22:1:6:1 (ginsenan PA) and 3:7:2:8:1 (ginsenan PB), in addition to small amounts of O-acetyl groups. Almost all (ginsenan PA) and part (ginsenan PB) of the hexuronic acid residues exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and periodate oxidation studies indicated that their structural features include mainly both alpha-arabino-beta-3,6-galactan type and rhamnogalacturonan type structural units. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test, pronounced anti-complementary activity and alkaline phosphatase-inducing activity in a dose dependent manner.
Tomoda
M
M
Kyoritsu College of Pharmacy, Tokyo, Japan.
Takeda
K
K
Shimizu
N
N
Gonda
R
R
Ohara
N
N
Takada
K
K
Hirabayashi
K
K
D000818:Animals; D002850:Chromatography, Gel; D003169:Complement Inactivator Proteins; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010084:Oxidation-Reduction; D005894:Panax / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects*
D003169:Complement Inactivator Proteins; D010936:Plant Extracts; D011134:Polysaccharides