Enzymatic determination of itoic acid, a Bacillus subtilis siderophore, and 2,3-dihydroxybenzoic acid.
A specific enzymatic method to determine the amounts of itoic acid, a Bacillus subtilis siderophore, and 2,3-dihydroxybenzoic acid (2,3-DHBA) was devised. A sample was incubated first with hippurate hydrolase and then with 2,3-DHBA-3,4-dioxygenase. Itoic acid was estimated from the increase in A374. The incubation with the first enzyme was omitted for the determination of 2,3-DHBA.
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Department of Biology, East Carolina University, Greenville, North Carolina 27858-4353.
D001412:Bacillus subtilis / Q000737:chemistry*; D006867:Hydrolases; D062385:Hydroxybenzoates / Q000032:analysis*; D010105:Oxygenases; D017262:Siderophores / Q000032:analysis*; D013053:Spectrophotometry
D062385:Hydroxybenzoates; D017262:Siderophores; C009135:2,3-dihydroxybenzoic acid; D010105:Oxygenases; D006867:Hydrolases