Molecular characterization of a mouse Y chromosomal repetitive sequence amplified in distantly related species in the genus Mus.
This report describes a 1.1 kb long mouse Y chromosomal sequence designated 142-4. It has a 42% GC content and is rich in short direct and inverted repeats. 142-4 related sequences are repeated about 200 times in the Mus musculus Y chromosome and their distribution was visualized by in situ hybridization. 142-4 detected a restriction fragment length polymorphism that differentiated between the M. m. musculus type and the M. m. domesticus type Y chromosome. Southern blot analysis of DNAs isolated from a panel of mouse species showed that 142-4 related sequences were amplified in the Y chromosomes of M. minutoides, M. musculus, M. saxicola, M. spicilegus, and M. spretus but not in those of M. caroli, M. cookii, and M. pahari. These results suggest that 142-4 related sequences are evolutionarily unstable and their accumulation patterns do not correlate with the known phylogenetic relationships of mouse species in the genus Mus.
Nishioka
Y
Y
Department of Biology, McGill University, Montréal, Que., Canada.
Dolan
B M
BM
Zahed
L
L
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D014998:Y Chromosome
D004247:DNA