Systemic induction of a potato pin2 promoter by wounding, methyl jasmonate, and abscisic acid in transgenic rice plants.
To address the question whether common signal(s) and transduction pathways are used to mediate a systemic wound response in monocot and dicot plants, a fusion of the potato proteinase inhibitor II gene (pin2) promoter and the bacterial beta-glucuronidase gene (Gus)-coding region was introduced into rice. In transgenic rice plants, the expression of the pin2-Gus fusion gene displays a systemic wound response, although the expression level is relatively low. Incorporation of the first intron from the rice actin 1 gene (Act1) into the 5'-untranslated region of the pin2-Gus construct results in high-level, systemically wound-inducible expression of the modified construct in transgenic rice plants. Histochemical analysis shows that this high-level, wound-inducible expression is associated with the vascular tissue in both leaves and roots. Furthermore, the expression of the pin2-Act1 intron-Gus fusion gene in transgenic rice plants can be systemically induced by both methyl jasmonate (MJ) and the phytohormone abscisic acid (ABA). These results suggest that the signal(s) mediating the observed systemic wound response and certain steps of the transduction pathways are conserved between dicot and monocot plants. Transient expression assays show that the pin2-Act1 intron-Gus construct is also actively expressed in transformed cells and tissues of several other monocot plants. Thus, the wound-inducible pin2 promoter in combination with the rice Act1 intron 1 might be used as an efficient regulator for foreign gene expression in transgenic monocot plants.
Xu
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Field of Botany, Cornell University, Ithaca, NY 14853.
McElroy
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Thornburg
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Wu
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D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D005075:Biological Evolution; D003517:Cyclopentanes / Q000494:pharmacology; D015870:Gene Expression; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D006651:Histocytochemistry; D007438:Introns / Q000235:genetics; D012275:Oryza / Q000502:physiology*; D054883:Oxylipins; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011480:Protease Inhibitors; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000235:genetics*; D013997:Time Factors; D014018:Tissue Distribution; D014170:Transformation, Genetic
D000085:Acetates; D000199:Actins; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D011480:Protease Inhibitors; D011993:Recombinant Fusion Proteins; C034104:proteinase inhibitor II protein, plant; D000040:Abscisic Acid; C072239:methyl jasmonate; D005966:Glucuronidase