Alteration in T cell subset phenotypes as cell markers for rejection or long-term allograft acceptance in allografted rats treated with antithymocyte globulin.
The alterations in the absolute levels of lymphocyte phenotype subsets were measured in a rat model for heart allograft acceptance induced by antithymocyte globulin (ATG). In untreated, allografted rats all T lymphocytes decreased with 50% within 3 days after transplantation and recovered within 1 week. ATG alone induced a depletion of 75% of the T lymphocytes, which did not normalise until 60 days after treatment. During the first 10 days, ATG-treated rats with an accepted allograft had higher levels of T lymphocytes than those with rejections while the T lymphocytes recovered faster after day 10 in the later rats. The study demonstrates different alterations in T lymphocytes phenotype subsets between rejecting rats and rats with long-term accepted allograft. It also shows that the operation trauma with rejection as well as the ATG treatment strongly influence the T cell subsets.
Olausson
M
M
Department of Surgery, Sahlgrenska Hospital, University of Göteborg, Sweden.
Wramner
L
L
Kjellson
B
B
Mjörnstedt
L
L
Söderström
T
T
D000818:Animals; D000961:Antilymphocyte Serum / Q000627:therapeutic use*; D005260:Female; D006084:Graft Rejection / Q000473:pathology*; D006085:Graft Survival / Q000187:drug effects*; D016027:Heart Transplantation / Q000276:immunology; D008297:Male; D008954:Models, Biological; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000187:drug effects; D014184:Transplantation, Homologous / Q000276:immunology
D000961:Antilymphocyte Serum