4-Hydroxyaminoquinoline 1-oxide can convert the Z-form of poly(dG-m5dC).poly(dG-m5dC) back to B-form-DNA.
Reactions between the chemical carcinogen 4-hydroxyaminoquinoline 1-oxide (4HAQO) and poly(dG-dC).poly(dG-dC), poly(dG-m5dC).poly(dG-m5dC) and poly(dG-br5dC).poly(dG-br5dC) were studied under B-form and Z-form conformation conditions. 4HAQO bound covalently to both B-form and Z-form-DNA. The extent of the B-form to Z-form-DNA transition was diminished by 4HAQO, as inferred by spectroscopic methods. Moreover, conversion of the Z-form-DNA back to a right handed DNA close to the B-form was observed with this carcinogen.
Suzuki
M
M
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Tada
M
M
D015108:4-Hydroxyaminoquinoline-1-oxide / Q000633:toxicity*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D015636:Magnesium Chloride / Q000494:pharmacology; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011089:Polydeoxyribonucleotides / Q000737:chemistry*
D011089:Polydeoxyribonucleotides; D015636:Magnesium Chloride; D015108:4-Hydroxyaminoquinoline-1-oxide; C029988:poly(dG-m(5)dC).poly(dG-m(5)dC); D004247:DNA