title: Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies.
abstract: Monoclonal antibodies (Mabs) were produced against crude scolex extract of T. solium metacestodes, and applied to ELISA-inhibition test for improving the specificity of serodiagnosis of human cysticercosis. Four hybridomas secreting species-specific anticysticercal Mabs (Cya-1, Cya-7, Cya-28 and Cya-31) were selected. Each Mab reacted on antigenic components of 25.5 kDa (Cya-1), 28 kDa (Cya-7), 87.5 kDa (Cya-28), and 12.5 kDa (Cya-31). IFA showed that Cya-1 was located at the calcium corpuscles, and Cya-7 at the loose connective tissue of T. solium metacestode scolex. Cya-28 and Cya-31 reacted on the tegument of the scolex. By conventional ELISA, 23 out of 28 (82.1%) cysticercosis patients were found serologically positive, but 1 out of 9 (11.1%) sparganosis cases and 6 out of 31 (19.4%) paragonimiasis cases showed false positives. By ELISA-inhibition test using species-specific anti-cysticercal Mab Cya-7, 19 out of 28 (67.9%) cysticercosis cases were found serologically positive, but there were no false positives in other parasitic infections.
authors.affiliation: Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: T S
authors.initials: TS
authors.lastname: Yong
authors.forename: I S
authors.initials: IS
authors.lastname: Yeo
authors.forename: J H
authors.initials: JH
authors.lastname: Seo
authors.forename: J K
authors.initials: JK
authors.lastname: Chang
authors.forename: J S
authors.initials: JS
authors.lastname: Lee
authors.forename: T S
authors.initials: TS
authors.lastname: Kim
authors.forename: G H
authors.initials: GH
authors.lastname: Jeong
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal