P- and E-cadherin are in separate complexes in cells expressing both cadherins.
E- and P-cadherin are members of a family of calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, the transmembrane cadherins self-associate, while intracellularly, they interact with the actin-based cytoskeleton. Several intracellular proteins, collectively termed catenins, are tightly associated with E- and P-cadherin. These proteins appear to link the cadherin to the cytoskeleton and have been proposed to be involved in concentrating cadherins at cell-cell adherens junctions. In this paper we report the production of monoclonal antibodies against both alpha- and beta-catenin and use these antibodies to show that in cells simultaneously expressing two different cadherins, E-cadherin and P-cadherin, each cadherin appears to be present in a separate cadherin/catenin complex.
Johnson
K R
KR
Department of Biology, University of Toledo, Ohio 43606.
Lewis
J E
JE
Li
D
D
Wahl
J
J
Soler
A P
AP
Knudsen
K A
KA
Wheelock
M J
MJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D015820:Cadherins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D002822:Choriocarcinoma / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured; D051178:alpha Catenin
D000911:Antibodies, Monoclonal; C495273:CTNNA1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D051178:alpha Catenin