The application of random amplified polymorphic DNA for sandfly species identification.
We have applied the recently developed Random Amplified Polymorphic DNA (RAPD) method to produce species-specific, DNA profiles for two sympatric, Venezuelan sandfly species, thought to be the vectors responsible for recent outbreaks of cutaneous and mucocutaneous leishmaniasis in the Andean State of Tachira. Moreover, within the profile, it was possible to identify a diagnostic DNA band for Lu. youngi of 0.32 kb. Results showed that the size of this diagnostic DNA band remained constant and did not vary with sex or geographical distribution.
Adamson
R E
RE
Liverpool School of Tropical Medicine, U.K.
Ward
R D
RD
Feliciangeli
M D
MD
Maingon
R
R
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005260:Female; D007303:Insect Vectors / Q000145:classification / Q000235:genetics; D007896:Leishmaniasis / Q000469:parasitology; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011576:Psychodidae / Q000145:classification* / Q000235:genetics
D004247:DNA