Age-related changes of DRG neuronal attachment to extracellular matrix proteins in vitro.
Attachment of dorsal root ganglion (DRG) neurones from 3-month-old (young adult) and 24-month-old (aged) mice to the following substrates was evaluated after 6 h in culture: (1) poly-L-lysine (PL), (2) PL + type I collagen (CL-I), (3) PL + type IV collagen (CL-IV), (4) PL + laminin (LM) and (5) PL + fibronectin (FN). In the young adult mice, each substrate coated onto PL, significantly increased the ratio of attachment, compared with PL alone. In the aged mice, CL-I and LM, but not CL-N or FN improved the attachment. There was no difference in cell survival or neurite extension after 48 h in culture between the two groups of mice. These results suggest that ageing causes selective changes of the neuronal cell-surface receptors respective to extracellular matrix proteins such as CL-IV and FN.
Sango
K
K
Department of Physiology, School of Medicine, Yokohama City University, Japan.
Horie
H
H
Inoue
S
S
Takamura
Y
Y
Takenaka
T
T
D000375:Aging / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D011107:Polylysine / Q000494:pharmacology
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D011107:Polylysine; D003094:Collagen