[Change in the permeability of liposome phospholipid membranes under the effect of Bacillus thuringiensis delta-endotoxins].
We studied release of the fluorescent calcein marker from egg-lecithin liposomes under action of intact delta-endotoxin from Bacillus thuringiensis var. israilensis, and hydrolysed preparation of delta-endotoxin from Bacillus thuringiensis var. kurstaki together with the motional parameters of 5- and 12-doxyl stearic radical probes in the liposomal membrane. The yield of the non-penetrating fluorescent marker was shown to be a sensitive indicator of the disturbance of the membrane barrier function.
Iarkov
S P
SP
Tret'iakov
S I
SI
Zlobin
V N
VN
D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D001427:Bacterial Toxins; D004731:Endotoxins / Q000494:pharmacology*; D005456:Fluorescent Dyes; D006460:Hemolysin Proteins; D008081:Liposomes; D008567:Membranes, Artificial; D010539:Permeability; D010743:Phospholipids / Q000378:metabolism*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D005456:Fluorescent Dyes; D006460:Hemolysin Proteins; D008081:Liposomes; D008567:Membranes, Artificial; D010743:Phospholipids; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Izmenenie pronitsaemosti fosfolipidnykh membran liposom pod deĭstviem preparatov delta-éndotoksinov Bacillus thuringiensis.