[Dynamics of growth and glucose uptake in cultured human fibroblasts collected from patients with diabetes mellitus, type 2].
Dynamics and glucose uptake in the human fibroblasts cultures were investigated for 14 days. Fibroblasts were taken from 16 patients with diabetes mellitus type 2 and 8 patients adjusted with age but with no history of diabetes mellitus. It was found that diabetic fibroblasts grew much slower than controls and reached the stationary phase later. Glucose uptake in both groups of fibroblasts--measured as the diminishment of glucose levels in the medium every two days--did not differ markedly in the phase of the rapid proliferation whereas was significantly higher in the stationary phase in the cultures of diabetic fibroblasts in comparison with the controls. It may thus assumed that diabetic fibroblasts reveal abnormal glucose metabolism in addition to reported intracellular disorders in protein, nucleic acids, lipids, collagen, and glycosaminoglycans synthesis.
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Zakładu Anatomii Patologicznej Instytutu Patologii AM, Lodzi.
D000328:Adult; D000368:Aged; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005947:Glucose / Q000493:pharmacokinetics*; D006801:Humans; D008875:Middle Aged
D005947:Glucose
Dynamika wzrostu i pochłanianie glukozy w hodowlach ludzkich fibroblastów pochodzacych od chorych na cukrzyce typu II.