title: Partial characterization of galactosyltransferase in human seminal plasma and its distribution in the human epididymis.
abstract: Galactosyltransferase activity has been partially characterized in human seminal plasma. Km values of 130 mumol l-1 for UDP-galactose and 2.25 mmol l-1 for N-acetylglucosamine were calculated and the enzyme was found to be dependent on temperature and manganese and present as a highly active component of human seminal plasma. Galactosyltransferase was inhibited by nucleotides, glycosylated nucleotides, bovine and human alpha-lactalbumin but not by monosaccharides. Radiation inactivation studies revealed that the biologically active unit of seminal plasma galactosyltransferase has a molecular mass of 45 kDa. Although the majority of galactosyltransferase activity found in seminal plasma is probably of prostatic origin, we report for the first time that it is also present in human epididymal intraluminal fluid. Low activity was detected in the proximal caput region but activity increased to maximum values in the adjacent downstream segment, the intermediate caput region. Specific activity was relatively constant albeit at a lower value in the following epididymal segments and vas deferens. The significance of the epididymal and seminal plasma galactosyltransferase activities is unknown, but the enzyme could be implicated in glycosylation events that are known to be important in gamete interaction.
authors.affiliation: Department of Biochemistry, University of Montreal, Québec, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Ross
authors.forename: N
authors.initials: N
authors.lastname: Vigneault
authors.forename: S
authors.initials: S
authors.lastname: Provencher
authors.forename: M
authors.initials: M
authors.lastname: Potier
authors.forename: K D
authors.initials: KD
authors.lastname: Roberts
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D004822:Epididymis / Q000378:metabolism*; D005700:Galactosyltransferases / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008297:Male; D051379:Mice; D012661:Semen / Q000201:enzymology*; D013094:Spermatozoa / Q000201:enzymology
chemical_list: D005700:Galactosyltransferases