[Differentiation of GC-rich restriction sites in the highly repeated DNA in Apodemus (Muridae, Rodentia].
nDNAs of six species of wood and field mice of the genus Apodemus (peninsulae, flavicolis, microps, speciosus, and agrarius), grey rat Rattus norvegicus as well, and house mouse Mus musculus were digested with restriction endonucleases (MspI (CCGG), BspRI (GGCC) and MvaI (CCA/TGG). Two highly representative satellites are found in A.peninsulae (8%) and M.musculus (20%) genomes using BspRI and MvaI. The length of restriction fragments of these components are equal to n x 30 and n x 230 nb, respectively. Based on the results of PDRF of nDNA in rodent interspecies, genetic distances are estimated and phylogenetic tree reconstructed. Among wood mice examined A.sylvaticus and A.flavicollos are most closely related, A.peninsulae is more diverged. Field mouse has approx. equal differences from the european and asian species of wood mice. A middle value of genetic distances is about 7.12% in Apodemus genus, 12.69 and 13.2% between Apodemus with M.musculus and R.norvegicus, respectively.
Chelomina
G N
GN
D000818:Animals; D001483:Base Sequence; D003596:Cytosine / Q000032:analysis*; D004247:DNA / Q000737:chemistry*; D006147:Guanine / Q000032:analysis*; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D010802:Phylogeny; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D006147:Guanine; D003596:Cytosine; D004247:DNA
Differentsiatsiia GTs-bogatykh saĭtov restriktsii v chastopovtoriaiushcheĭsia DNK Apodemus (Muridae, Rodentia).