Does topical hydrogen peroxide penetrate the cornea?
OBJECTIVE
To determine under what conditions hydrogen peroxide (H2O2) can penetrate the cornea and cause increased concentrations of H2O2 in the aqueous humor.


METHODS
Rabbit corneas were exposed in vitro and in vivo to H2O2 concentrations up to 60 mmol/l either in a 600 microliter volume or as consecutive drops. H2O2 was measured over time either in the endothelial superfusate or in tapped samples of aqueous humor, and in the fluid applied to the ocular surface. The stability of exogenous H2O2 added directly to aqueous humor was also determined.


RESULTS
Exogenous H2O2 in aqueous humor decays with a half-life of 20 minutes, chiefly as a result of catalase activity. When applied to the entire ocular surface, 600 microliters of 60 mmol/l H2O2 caused no change in the concentration in aqueous humor, but when applied to the corneal surface alone, penetration occurred at 18 mmol/l and above. When applied as eight 40-microliters drops to the ocular surface the threshold for H2O2 penetration was above 36 mmol/l.


CONCLUSIONS
H2O2 is rapidly eliminated on the ocular surface, chiefly by enzyme activity of the conjunctiva and cornea. Threshold for penetration of H2O2 into the aqueous humor depends on volume, concentration, duration, and ocular surface exposed. In a healthy eye, exogenously derived H2O2 is eliminated by enzyme activity of the aqueous humor and tissues surrounding the anterior chamber.
Wilson
G
G
Vision Science Research Center, School of Optometry, University of Alabama, Birmingham 35294-4390.
Riley
M V
MV
D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D003315:Cornea / Q000378:metabolism*; D004355:Drug Stability; D006207:Half-Life; D006861:Hydrogen Peroxide / Q000493:pharmacokinetics*; D009883:Ophthalmic Solutions; D011817:Rabbits
D009883:Ophthalmic Solutions; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid