Monoclonal antibody-based detection system for Salmonella enteritidis.
Two monoclonal antibodies that react with Salmonella enteritidis in chicken tissue, eggs, and environmental samples were used to develop a rapid enzyme-linked immunosorbent assay (ELISA) screen and agglutination assay for the specific detection of S. enteritidis. S. enteritidis was detected in 100% of egg samples and 99.8% of various field and research samples by both ELISA and traditional microbiological isolation and identification techniques. Results of titer experiments indicate that as few as 10(4) organisms can be detected by ELISA.
Keller
L H
LH
School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, Kennett Square 19348.
Benson
C E
CE
Garcia
V
V
Nocks
E
E
Battenfelder
P
P
Eckroade
R J
RJ
D000818:Animals; D000911:Antibodies, Monoclonal; D001431:Bacteriological Techniques / Q000662:veterinary; D002645:Chickens / Q000382:microbiology*; D004531:Eggs / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal