Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants.
Two versatile Clostridium perfringens-Escherichia coli shuttle vectors were constructed. Each plasmid carried a single antibiotic resistance gene which was expressed in both organisms. The plasmid pJIR750 encoded resistance to chloramphenicol and pJIR751 encoded resistance to erythromycin. Each plasmid contained the pUC18-derived multiple cloning site and the lacZ' gene which enabled direct screening for recombinants in E. coli. These plasmids should prove invaluable for the genetic manipulation of C. perfringens.
Bannam
T L
TL
Department of Microbiology, Monash University, Clayton, Australia.
Rood
J I
JI
D002702:Chloramphenicol Resistance / Q000235:genetics; D003016:Clostridium perfringens / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors; D011815:R Factors
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004917:Erythromycin