Characterization of a novel activation-specific antiplatelet monoclonal antibody.
We developed a murine monoclonal antibody (KmT-50) which binds to a protein expressed on the surface of human platelets after activation. KmT-50 is an immunoglobulin G1 monoclonal antibody that recognizes a 50-kD antigen localized in intracellular organelles and the open canalicular system of unstimulated platelets. After platelets were stimulated, the antigen was secreted via the surface-connected canalicular system and exposed on the platelet membrane. KmT-50 bound not only to human platelets, but also to rabbit and monkey platelets. This new antibody may be useful for experimental studies on thrombosis and hemostasis.
Fukuroi
T
T
First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Kawakatsu
T
T
Nomura
S
S
Hamamoto
K
K
Kido
H
H
Yamaguchi
K
K
Suzuki
M
M
Yanabu
M
M
Soga
T
T
Kokawa
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D016824:Antigens, Human Platelet / Q000276:immunology* / Q000378:metabolism; D001606:Bernard-Soulier Syndrome / Q000097:blood; D001792:Blood Platelets / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D015388:Organelles / Q000276:immunology; D015539:Platelet Activation; D013915:Thrombasthenia / Q000097:blood
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D007074:Immunoglobulin G; D008565:Membrane Proteins