Impairment of fibrinolysis during the growth of two murine mammary adenocarcinomas.
The fibrinolytic activity present in the euglobulin (EU) fraction of BALB/c mice before and during the growth of M3 and MM3 murine mammary adenocarcinomas was characterized. The main plasminogen activator (PA) form contained in EUs from control mice was defined as murine urokinase-type PA (uPA). Overall fibrinolytic activity decreased significantly during tumor development. Zymographies showed that this fall was associated with a reduction in the free uPA band (47 kDa) and to the detection of a tissue-type PA (tPA) complexed band (117 kDa). Western blotting showed free tPA protein (68 kDa) in control mice, that disappeared in M3 tumor-bearing mice. In this model, high subcutaneous tumor burden induces a severe impairment in the circulating fibrinolytic system.
Alonso
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DF
Research Department, Institute of Oncology Angel H. Roffo, University of Buenos Aires, Argentina.
Farías
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EF
Bal de Kier Joffé
E
E
D000230:Adenocarcinoma / Q000097:blood* / Q000201:enzymology / Q000473:pathology / Q000556:secondary; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005342:Fibrinolysis / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D008175:Lung Neoplasms / Q000201:enzymology / Q000556:secondary; D008325:Mammary Neoplasms, Experimental / Q000097:blood* / Q000201:enzymology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D010960:Plasminogen Activators / Q000097:blood*; D015849:Plasminogen Inactivators / Q000097:blood; D010959:Tissue Plasminogen Activator / Q000097:blood; D014568:Urokinase-Type Plasminogen Activator / Q000097:blood
D015849:Plasminogen Inactivators; D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator