A novel method employing UNG to avoid carry-over contamination in RNA-PCR.
Udaykumar
Laboratory of Molecular Virology, FDA/CBER, Rockville, MD 20852-1448.
Epstein
J S
JS
Hewlett
I K
IK
D002460:Cell Line; D045647:DNA Glycosylases; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000302:isolation & purification*; D051981:Uracil-DNA Glycosidase
D012367:RNA, Viral; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase