Efficient processing and export of human growth hormone by heat labile enterotoxin chain B signal sequence.
The heat-labile enterotoxin chain B (LTB) signal sequence was used for the processing and export of human growth hormone (hGH). The protein was completely processed and exported across the cell membrane to accumulate in the periplasmic space in Escherichia coli. The human growth hormone cDNA was cloned as a PCR amplified fragment under the control of tac promoter and translationally fused to the LTB signal sequence. The rate of processing of hGH under the control of the LTB signal sequence was equal to or more than the rate of induction of expression, indicating efficient processing. The receptor binding activity of the processed periplasmic protein was established in a radio receptor assay.
Ghorpade
A
A
Gene Regulation Laboratory, National Institute of Immunology, New Delhi, India.
Garg
L C
LC
D001427:Bacterial Toxins / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004768:Enterotoxins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000378:metabolism*; D011869:Radioligand Assay; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001427:Bacterial Toxins; D004274:DNA, Recombinant; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D013006:Growth Hormone; C027538:heat-labile enterotoxin, E coli