Studies on inhibitors of skin tumor promotion, XII. Rotenoids from Amorpha fruticosa.
As a part of screening studies for chemopreventive agents (anti-tumor-promoters), six North American plants belonging to the Amorpha genus were tested using an in vitro assay system. Of these plants, Amorpha fruticosa exhibited strong inhibitory effects on Epstein-Barr virus early antigen (EBA-EA) activation induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Also six rotenoids, amorphispironone [1], tephrosin [2], amorphigenin [3], 12a-hydroxyamorphigenin [4], 12a-hydroxydalpanol [5], and 6'-O-D-glucopyranosyldalpanol [6], were isolated from the leaves of A. fruticosa. Among these retenoids, 1 and 2 exhibited remarkable inhibitory effects of EBV-EA activation induced by TPA. Further, 1 and 2 exhibited significant anti-tumor-promotion effects on mouse skin tumor promotion in an in vivo two-stage carcinogenesis test. These investigations suggested that these rotenoids might be valuable anti-tumor-promoters.
Konoshima
T
T
Kyoto Pharmaceutical University, Japan.
Terada
H
H
Kokumai
M
M
Kozuka
M
M
Tokuda
H
H
Estes
J R
JR
Li
L
L
Wang
H K
HK
Lee
K H
KH
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D016588:Anticarcinogenic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D000956:Antigens, Viral / Q000276:immunology; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006586:Hexanes; D000432:Methanol; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry; D012402:Rotenone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; D006586:Hexanes; D010936:Plant Extracts; D012402:Rotenone; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate; D000432:Methanol