Antihistone antibodies in patients with localized scleroderma.
OBJECTIVE
To study the prevalence and antigen specificity of antihistone antibodies (AHA) in localized scleroderma.


METHODS
Forty-nine serum samples from patients with localized scleroderma were examined by an enzyme-linked immunosorbent assay (ELISA) and by immunoblotting.


RESULTS
By ELISA, AHA were demonstrated in 47% (23 of 49) of patients with localized scleroderma and in 87% (13 of 15) of patients with generalized morphea. Immunoblotting revealed that the predominant antigens were histones H1 and H3. The presence of AHA correlated with that of anti-single-stranded DNA antibody.


CONCLUSIONS
Some of the major antigens for antinuclear antibodies in patients with localized scleroderma are histones.
Sato
S
S
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Ihn
H
H
Soma
Y
Y
Igarashi
A
A
Tamaki
T
T
Kikuchi
K
K
Ishibashi
Y
Y
Takehara
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D000974:Antibodies, Antinuclear / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006657:Histones / Q000097:blood*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012594:Scleroderma, Localized / Q000097:blood / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D006657:Histones; D007074:Immunoglobulin G; D007075:Immunoglobulin M