Involvement of RET oncogene in human tumours: specificity of RET activation to thyroid tumours.
Non-thyroid neoplasia were analysed by Southern blot of genomic DNA and DNA prepared by reverse transcription and amplification by polymerase chain reaction (RT/PCR) for the activation of the RET oncogene. It is known that the rearrangement of RET occurs in about 10%-20% of human thyroid papillary carcinomas. None of 528 non-thyroid tumours showed rearrangement of the RET proto-oncogene, whereas three out of 30 thyroid papillary carcinomas were positive for RET activation. Therefore the activation of RET seems to be a somatic cell mutation specific to human thyroid carcinomas.
Santoro
M
M
Centro di Endocrinologia ed Oncologia Sperimentale del CNR, Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Sabino
N
N
Ishizaka
Y
Y
Ushijima
T
T
Carlomagno
F
F
Cerrato
A
A
Grieco
M
M
Battaglia
C
C
Martelli
M L
ML
Paulin
C
C
D001483:Base Sequence; D015139:Blotting, Southern; D002291:Carcinoma, Papillary / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D009857:Oncogenes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000032:analysis; D013964:Thyroid Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger