Isolation, purification, toxicity and some physicochemical properties of mycotoxins produced by aspergillus quadrilineatus isolated from acha (Digitaria exilis stapf) in Plateau State of Nigeria.
Some physico-chemical and toxicological studies were carried out on mycotoxins elaborated by Aspergillus quadrilineatus isolated from a grain foodstuff, acha (Digitaria exilis) in the Plateau State of Nigeria. The mycotoxins produced by A quadrilineatus were extractable from rice culture by chloroform. Column chromatographic separations of the crude extract in silica gel using different elution solvents and biological tests showed that the mycotoxins came off in the diethylether, chloroform and mostly in the ethyl acetate fractions. Use of different available mycotoxin standards on silica gel G coated chromatoplates revealed that 1 of the mycotoxins produced by A quadrilineatus was sterigmatocystin. Two other more toxic mycotoxins were isolated and purified from the crude chloroform extract; their column, preparative thin-layer chromatographic, infrared and UV-spectrophotometric characteristics were established. The infrared spectra of the 2 purified mycotoxins suggested that the carbonyl group of their structures were similar to that of aflatoxin.
Gbodi
T A
TA
Federal University of Technology, Department of Biological Sciences, Minna, Niger State, Nigeria.
D000818:Animals; D001230:Aspergillus / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D005516:Food Microbiology; D051379:Mice; D009183:Mycotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D012275:Oryza / Q000382:microbiology*; D013057:Spectrum Analysis
D009183:Mycotoxins