title: Ectopic neural expression of a floor plate marker in frog embryos injected with the midline transcription factor Pintallavis.
abstract: The floor plate, a cell group that develops at the midline of the neural plate in response to inductive signals from the notochord, has been implicated in the control of dorsoventral neural pattern. The frog Pintallavis gene, encoding a member of the HNF-3/fork head transcription factor family, is expressed in the notochord and in midline neural plate cells that give rise to the floor plate. To examine whether Pintallavis might be involved in regulating the differentiation of the floor plate, we ectopically expressed Pintallavis by injection of synthetic mRNA into two-cell frog embryos. Injection of Pintallavis mRNA resulted in the ectopic expression of F-spondin, a gene encoding a floor plate-specific adhesion molecule, at the dorsal midline of the neural tube. The expression of Pintallavis in midline cells may therefore contribute to the establishment of the floor plate fate.
authors.affiliation: Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.
authors.forename: A
authors.initials: A
authors.lastname: Ruiz i Altaba
authors.forename: C
authors.initials: C
authors.lastname: Cox
authors.forename: T M
authors.initials: TM
authors.lastname: Jessell
authors.forename: A
authors.initials: A
authors.lastname: Klar
mesh_terms: D000417:Albinism; D000595:Amino Acid Sequence; D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000502:physiology*; D051858:Forkhead Transcription Factors; D015723:Gene Library; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009474:Neurons / Q000502:physiology*; D010455:Peptides; D012333:RNA, Messenger / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D019699:Thrombospondins; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D015815:Cell Adhesion Molecules; D051858:Forkhead Transcription Factors; C000598904:FoxA4 protein, Xenopus; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D010455:Peptides; D012333:RNA, Messenger; D019699:Thrombospondins; D014157:Transcription Factors; D029867:Xenopus Proteins; C498958:spon1 protein, Xenopus