title: Voltage gradient electrophoresis of nucleic acids on agarose gels.
abstract: A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about 30 kb) in the same electrophoresis agarose gel. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, submerged in the electrophoretic running buffer. Further applications of our system are also described.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Barbieri
authors.forename: V
authors.initials: V
authors.lastname: Izzo
authors.forename: M A
authors.initials: MA
authors.lastname: Costa
authors.forename: G
authors.initials: G
authors.lastname: Giudice
authors.forename: G
authors.initials: G
authors.lastname: Duro
mesh_terms: D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D008970:Molecular Weight; D010957:Plasmids / Q000302:isolation & purification
chemical_list: D004247:DNA