In vivo gene delivery. Efficient transfection of T lymphocytes in adult mice.
We have demonstrated that a single intraperitoneal injection of cationic liposomes complexed to a chloramphenicol acetyltransferase (CAT) gene expression plasmid can transfect the majority of splenic Thy 1.2+ T lymphocytes, as well as significant numbers of bone marrow-derived hematopoietic cells, in adult mice. CAT activity was detected in the spleen for at least 2 weeks, and there was no evidence of treatment-related toxicity. Some degree of tissue-specific transgene expression was achieved by varying the cationic lipid used.
Philip
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R
Cancer Research Institute, University of California, San Francisco 94143-0128.
Liggitt
D
D
Philip
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M
Dazin
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P
Debs
R
R
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D004247:DNA; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism*; D014162:Transfection
D008081:Liposomes; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase