Use of immunoelectron microscopy to demonstrate Francisella tularensis.
Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potentially be useful for demonstrating other medically important bacteria.
Geisbert
T W
TW
Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011.
Jahrling
P B
PB
Ezzell
J W
JW
D000946:Antigens, Fungal; D005069:Evaluation Studies as Topic; D005604:Francisella tularensis / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000379:methods*; D013045:Species Specificity; D014406:Tularemia / Q000175:diagnosis
D000946:Antigens, Fungal