Single-pass gill extraction and tissue distribution of atrial natriuretic peptide in trout.
A new method is described in the present experiments to quantify atrial natriuretic peptide (125I-ANP) extraction from plasma in a single transit through the gill vasculature of an unanesthetized trout. Tissue distribution of 125I relative to an inert extracellular space marker, 58Co-EDTA, was also measured 1 h after injection of 125I-ANP. Single-pass extraction of 125I-ANP by the gill was 60% in control fish; it fell to 18% in fish previously injected with saline and became negative (relative to 58Co-EDTA) after treatment with the clearance (C-type) receptor inhibitor SC 46542. Approximately 90% of a 125I-ANP bolus injected into control trout is removed from the circulation within 2 min. 125I concentration in gill tissue was nearly seven times greater than that predicted by the 58Co-EDTA space. 125I was also concentrated in brain (2X), gallbladder, gastrointestinal tract, and eye (all 1X). SC 46542 decreased gill 125I binding from seven times to one times the 58Co-EDTA space and increased 125I accumulation in heart, kidney, fat, and skeletal muscle. Extraction of 125I-ANP by the isolated perfused gill was 53%, similar to that observed in vivo. These results show that the gill is a major site for ANP removal from trout plasma and that C-type receptors are predominant in this process.
Olson
K R
KR
Indiana University School of Medicine, South Bend Center, University of Notre Dame 46556.
Duff
D W
DW
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D005880:Gills / Q000378:metabolism*; D009994:Osmolar Concentration; D013997:Time Factors; D014018:Tissue Distribution; D014337:Trout / Q000378:metabolism*
D009320:Atrial Natriuretic Factor