Chemoenzymatic galactosialylation with integrated cofactor regeneration.
As a precursor for the chemical synthesis of sialylated oligosaccharides, the trisaccharide glycoside Neu5Ac alpha (2-8)Gal beta (1-4)GlcNAc beta (1-O)-pent-4-ene was synthesized starting from GlcNAc beta (1-O)-pent-4-ene, UDP-glucose and N-acetylneuraminic acid in a one pot reaction employing galactosyltransferase and alpha (2-6)sialyltransferase in a complete cofactor regeneration system.
Stangier
P
P
Institut für Organische Chemie, Universität Hamburg, Germany.
Treder
W
W
Thiem
J
J
D002240:Carbohydrate Sequence; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000138:chemical synthesis*; D005700:Galactosyltransferases; D008969:Molecular Sequence Data; D015394:Molecular Structure; D000216:N-Acylneuraminate Cytidylyltransferase / Q000302:isolation & purification; D012799:Sialyltransferases / Q000302:isolation & purification*; D014312:Trisaccharides / Q000138:chemical synthesis*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D014312:Trisaccharides; C082759:N-acetylneuraminyl-2-6-galactopyranosyl-1-4-N-acetylglucosaminyl-1-O-pent-4-ene; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D005700:Galactosyltransferases; D012799:Sialyltransferases; D000216:N-Acylneuraminate Cytidylyltransferase; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase