title: Biosynthesis and secretion of a precursor of nisin Z by Lactococcus lactis, directed by the leader peptide of the homologous lantibiotic subtilin from Bacillus subtilis.
abstract: The DNA sequence encoding the leader peptide of the lantibiotic subtilin from Bacillus subtilis was fused to the sequence encoding pronisin Z, and this hybrid gene was expressed in a Lactococcus lactis strain that produces nisin A. This strain simultaneously secreted nisin A and a protein of approximately 6 kDa. Amino acid sequencing of the purified 6 kDa protein and structural analysis of its main tryptic fragment by two-dimensional 1H-NMR showed that it consists of the unmodified leader peptide of subtilin, without the N-terminal methionine residue, linked to a fully matured nisin Z part. The hybrid protein and its main tryptic fragment [ITPQ]-nisin Z, showed at least 200-fold lower antimicrobial activities than nisin Z against three different indicator strains.
authors.affiliation: Department of Biophysical Chemistry, NIZO, Ede, The Netherlands.
authors.forename: O P
authors.initials: OP
authors.lastname: Kuipers
authors.forename: H S
authors.initials: HS
authors.lastname: Rollema
authors.forename: W M
authors.initials: WM
authors.lastname: de Vos
authors.forename: R J
authors.initials: RJ
authors.lastname: Siezen
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins; D001430:Bacteriocins; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D013294:Lactococcus lactis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009561:Nisin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D010455:Peptides; D010456:Peptides, Cyclic / Q000235:genetics / Q000378:metabolism; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014357:Trypsin
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D004269:DNA, Bacterial; D010455:Peptides; D010456:Peptides, Cyclic; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D009561:Nisin; D014357:Trypsin; C002836:subtilin; C071364:nisin Z