Laser-induced fluorescence in malignant and normal tissue of rats injected with benzoporphyrin derivative.
Laser-induced fluorescence was used to characterize the localization of intravenously administered benzoporphyrin derivative-monoacid (BPD-MA) 3 h postinjection in different rat tissue types, including an induced experimental malignant tumor. A comparison of the fluorescence properties and demarcation potential between the newer sensitizer BPD-MA and four other substances, hematoporphyrin (HP), polyhematoporphyrin ester (PHE), tetrasulfonated phthalocyanine (TSPc) and the commercially available Photofrin earlier investigated, is included. The fluorescence light was induced with a nitrogen laser, emitting at 337 nm. The fluorescence spectrum in the region 380-750 nm was analyzed by a polychromator equipped with a diode array detector. The demarcation potential between tumor and surrounding tissue in terms of fluorescence signal for the tumor model used was 2:1 for BPD-MA. In comparison with the other drugs, HP shows about the same demarcation potential, whereas Photofrin and PHE exhibit about 3 times better and TSPc about 1.5 times better demarcation. By also employing the endogenous tissue fluorescence signature the contrast was enhanced by a factor of about 2 for each of the five drugs.
Andersson-Engels
S
S
Department of Physics, Lund Institute of Technology, Sweden.
Ankerst
J
J
Johansson
J
J
Svanberg
K
K
Svanberg
S
S
D000818:Animals; D005453:Fluorescence; D007834:Lasers; D008297:Male; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D011166:Porphyrins / Q000493:pharmacokinetics; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics; D051381:Rats; D011920:Rats, Inbred WF; D014018:Tissue Distribution
D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C504224:benzoporphyrin D