title: Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion.
abstract: Salmonella typhimurium and enteropathogenic Escherichia coli (EPEC) were found to adhere to the brush border of differentiated human intestinal epithelial Caco-2 cells in culture, whereas Yersinia pseudotuberculosis and Listeria monocytogenes adhered to the periphery of undifferentiated Caco-2 cells. All these enterovirulent strains invaded the Caco-2 cells. Using a heat-killed human Lactobacillus acidophilus (strain LB) which strongly adheres both to undifferentiated and differentiated Caco-2 cells, we have studied inhibition of cell association with and invasion within Caco-2 cells by enterovirulent bacteria. Living and heat-killed Lactobacillus acidophilus strain LB inhibited both cell association and invasion of Caco-2 cells by enterovirulent bacteria in a concentration-dependent manner. The mechanism of inhibition of both adhesion and invasion appears to be due to steric hindrance of human enterocytic pathogen receptors by whole-cell lactobacilli rather than to a specific blockade of receptors.
authors.affiliation: Département de Microbiologie et Immunologie, UFR de Sciences Pharmaceutiques Paris XI, Châtenay-Malabry, France.
authors.forename: M H
authors.initials: MH
authors.lastname: Coconnier
authors.forename: M F
authors.initials: MF
authors.lastname: Bernet
authors.forename: S
authors.initials: S
authors.lastname: Kernéis
authors.forename: G
authors.initials: G
authors.lastname: Chauvière
authors.forename: J
authors.initials: J
authors.lastname: Fourniat
authors.forename: A L
authors.initials: AL
authors.lastname: Servin
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001667:Binding, Competitive; D002478:Cells, Cultured; D004755:Enterobacteriaceae / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology / Q000648:ultrastructure; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000382:microbiology* / Q000648:ultrastructure; D007779:Lactobacillus acidophilus / Q000502:physiology* / Q000648:ultrastructure; D008089:Listeria monocytogenes / Q000502:physiology* / Q000648:ultrastructure; D008871:Microvilli / Q000382:microbiology* / Q000648:ultrastructure; D012486:Salmonella typhimurium / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure; D015011:Yersinia pseudotuberculosis / Q000502:physiology / Q000648:ultrastructure