title: No effect of human serum and erythrocytes enriched in n-3 fatty acids by oral intake on Plasmodium falciparum blood stage parasites in vitro.
abstract: To examine the effect of n-3 polyunsaturated fatty acids (n-3 PUFA) on the erythrocytic growth of Plasmodium falciparum, serum and erythrocytes were separated from blood of a healthy donor before and after he had taken fish oil capsules for 8 days. Such intake supplied an amount of eicosapentaenoic acid (EPA, 20:5n-3) of 3.5 g/d and docosahexaenoic acid (DHA, 22:6n-3) of 2.5 g/d and 24 mg/d of total tocopherol. Post-intake fish oil serum (post-s) and erythrocytes (post-e) were tested in vitro for inhibitory activity against blood stages of P. falciparum compared with pre-intake serum (pre-s) and pre-intake erythrocyte (pre-e). Also the effect of EPA and arachidonic acid (AA, 20:4n-6) on the erythrocytic growth of P. falciparum was tested using in vitro assays. The results show that both post-s and post-e had no antimalarial activity on P. falciparum. No differential antimalarial effect was observed for 20:5n-3 compared with 20:4n-6, which at high concentrations (> 40 microM) had an anti-schizont-growth effect.
authors.affiliation: Department of Infectious Diseases, University Hospital (Rigshospitalet), Copenhagen, Denmark.
authors.forename: Y A
authors.initials: YA
authors.lastname: Abu-Zeid
authors.forename: H S
authors.initials: HS
authors.lastname: Hansen
authors.forename: P H
authors.initials: PH
authors.lastname: Jakobsen
authors.forename: I C
authors.initials: IC
authors.lastname: Bygbjerg
authors.forename: J
authors.initials: J
authors.lastname: Dyerberg
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001790:Blood Physiological Phenomena; D002455:Cell Division; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D008297:Male; D010963:Plasmodium falciparum / Q000187:drug effects / Q000254:growth & development*; D014280:Triglycerides / Q000494:pharmacology*
chemical_list: D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D014280:Triglycerides; C077446:Pikasol; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid