Inhibition of progesterone action by a factor in human follicular fluid.
A factor was found in human follicular fluid that blocked progesterone-stimulated net uptake of 45Ca2+ in human sperm and progesterone-induced maturation of Xenopus oocyte. The factor was partially purified by ultrafiltration through PM-10 membrane and gel filtration on Sephadex G-25 column. The active fraction is effective at a concentration of 200 micrograms/ml. The present findings suggest that hFF factor may regulate the metabolism of progesterone sensitive cells.
Juneja
R
R
Center for Biomedical Research, Population Council, New York, New York.
Kadam
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P
Kadam
A L
AL
Koide
S S
SS
D000818:Animals; D002132:Calcium Radioisotopes / Q000378:metabolism; D002850:Chromatography, Gel; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D011374:Progesterone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D014462:Ultrafiltration; D014981:Xenopus
D002132:Calcium Radioisotopes; D011374:Progesterone