title: Bio-metabolism of green S and indigo carmine through caecal microflora of rats.
abstract: Bio-metabolism of two permitted food colours viz., Green S and Indigo Carmine, through caecal microflora of rats was investigated. Incubation of Green S with caecal flora resulted in one coloured and two fluorescent metabolites with the corresponding Rf values of 0.83, 0.33, 0.22, respectively. Indigo carmine was metabolized by caecal microflora to four [corrected] fluorescent metabolites with Rf values of 0.09, 0.27, 0.5 and 0.72. The DI 50 value of Green S was considerably higher (456 min) as compared to that of Indigo carmine (54 min). These results suggest that Green S and Indigo carmine are biotransformed to a variety of metabolites by rat caecal microflora.
authors.affiliation: Dyes & Food Adulterant Toxicology Laboratory Industrial Toxicology Research Centre, Lucknow, India.
authors.forename: S
authors.initials: S
authors.lastname: Singh
authors.forename: M
authors.initials: M
authors.lastname: Das
authors.forename: S K
authors.initials: SK
authors.lastname: Khanna
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D002432:Cecum / Q000382:microbiology*; D005505:Food Coloring Agents / Q000378:metabolism; D005766:Gastrointestinal Contents; D007203:Indigo Carmine / Q000378:metabolism*; D007700:Kinetics; D008086:Lissamine Green Dyes / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005505:Food Coloring Agents; D008086:Lissamine Green Dyes; C024537:green 5; D007203:Indigo Carmine