PCR amplification of partial mRNA sequences encoding the alpha- and beta-globin chains of the bivalve mollusc Anadara trapezia: correction of the C-terminal amino acid sequence of the alpha-chain.
1. PCR was carried out on cDNA which had been synthesized from poly(A)+ RNA extracted from A. trapezia red blood cells. 2. A 348 bp product encoding the C-terminal region of the alpha-globin chain and another product encoding a portion of the beta-globin chain were isolated. 3. The C-terminal alpha-chain sequence, encoded by the cDNA, differs from the previously published sequence and these changes improve the alignment of this chain with the other globin chains of this organism.
Nassif
N T
NT
School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia.
Mackinlay
A G
AG
Thompson
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EO
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005914:Globins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D008974:Mollusca / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D005914:Globins; D004247:DNA