Purification and partial characterization of a product from Yersinia pseudotuberculosis with the ability to activate human T cells.
We tried to purify a substance exhibiting mitogenicity for human peripheral blood lymphocytes from Yersinia pseudotuberculosis isolated from patients with Y. pseudotuberculosis infection manifesting acute and systemic clinical symptoms. The supernatant of a suspension of the bacteria disrupted by sonication was serially chromatographed on DEAE-Sepharose fast-flow, Sephacryl S-100 HR, and TSK-gel G2000SW high-pressure liquid chromatography columns. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that the purified mitogenically active substance migrated as a single band corresponding to a molecular mass of 21 kDa. We designated the purified substance Y. pseudotuberculosis-derived mitogen (YPM). YPM stimulated human peripheral T cells to proliferate and produce interleukin-2 at 0.1 ng/ml or more. YPM-induced T-cell activation required the expression of HLA class II molecules on accessory cells.
Miyoshi-Akiyama
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Department of Microbiology and Immunology, Tokyo Women's Medical College, Japan.
Imanishi
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Uchiyama
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D000938:Antigen-Presenting Cells / Q000502:physiology; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000502:physiology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008934:Mitogens / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D015011:Yersinia pseudotuberculosis / Q000737:chemistry*
D001426:Bacterial Proteins; D000949:Histocompatibility Antigens Class II; D008934:Mitogens; C093313:Yersinia pseudotuberculosis-derived mitogen