Cytogenetic and fluorescence in situ hybridization investigation of ring chromosomes characterizing a specific pathologic subgroup of adipose tissue tumors.
Cytogenetic analysis of 184 adipose tissue tumors, 175 lipomas, and nine liposarcomas (LPS) showed the presence of a ring chromosome and/or a long marker chromosome in 10 cases with common histologic features such as atypical stromal cells with or without lipoblasts. In five of the cases, this appeared to be the sole cytogenetic abnormality. Fluorescence in situ hybridization (FISH) analysis with a microclone library specific for chromosome region 12q13-q15 showed extensive staining of the ring and long marker chromosomes, indicating that genetic sequences of this particular region of chromosome 12 are present in these marker chromosomes, most likely in an amplified form.
Dal Cin
P
P
Center for Human Genetics, University of Leuven, Belgium.
Kools
P
P
Sciot
R
R
De Wever
I
I
Van Damme
B
B
Van de Ven
W
W
Van den Berghe
H
H
D000273:Adipose Tissue / Q000473:pathology*; D000368:Aged; D002871:Chromosome Banding; D002881:Chromosomes, Human, Pair 12; D005260:Female; D005819:Genetic Markers; D005834:Genital Neoplasms, Male / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008067:Lipoma / Q000235:genetics* / Q000648:ultrastructure; D008080:Liposarcoma / Q000235:genetics* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D012186:Retroperitoneal Neoplasms / Q000235:genetics; D012303:Ring Chromosomes; D012983:Soft Tissue Neoplasms / Q000235:genetics; D013085:Spermatic Cord; D013848:Thigh
D005819:Genetic Markers