Analysis of chondroitin sulfate/dermatan sulfate chains in rat peritoneal resident macrophages.
Chondroitin sulfate/dermatan sulfate (CS/DS) hybrid polymers obtained from rat peritoneal resident macrophages were analyzed by the enzymatic method; CS/DS chains were digested by chondroitinase, and the resultant unsaturated disaccharides were determined by HPLC with fluorometric detection. CS/DS in the resident macrophages are constituted of Di-4S, Di-6S, Di-diSE, Di-diSB and Di-triS.
Koshiishi
I
I
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Hayashi
S
S
Imanari
T
T
D000818:Animals; D002809:Chondroitin Sulfates / Q000032:analysis*; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D003871:Dermatan Sulfate / Q000032:analysis*; D006868:Hydrolysis; D008264:Macrophages / Q000737:chemistry*; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence
D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases