title: Characterization of immunotrap assays for urokinase plasminogen activator and its inhibitors and measurements of these molecules in human plasma and mouse macrophage in culture.
abstract: 1. Immunotrap assays that can measure the activities of urokinase-type plasminogen activator (uPA) and its inhibitors (PAIs) were characterized. 2. Both human plasma and mouse macrophages in culture were found to contain much higher inhibitor activity than uPA-like activity. 3. The balance between pro- and anti-fibrinolytic activities was quantitatively changed in the murine macrophages after the injection of thioglycollate. uPA-like materials were synthesized by the macrophages and secreted to the conditioned medium continuously, while PAI activity was unchanged during the same time period.
authors.affiliation: Department of Biochemistry, University of Hong Kong.
authors.forename: S K
authors.initials: SK
authors.lastname: Kung
authors.forename: H K
authors.initials: HK
authors.lastname: Lau
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D006801:Humans; D007118:Immunoassay; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D015849:Plasminogen Inactivators / Q000032:analysis / Q000097:blood; D011817:Rabbits; D013864:Thioglycolates / Q000494:pharmacology; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000097:blood
chemical_list: D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D015849:Plasminogen Inactivators; D013864:Thioglycolates; D014568:Urokinase-Type Plasminogen Activator