title: Association of annexin V with mitochondria.
abstract: Annexin V is an intracellular protein recently shown to be localized to nucleoli and cytosol. In this study we show that cytosolic annexin V is associated with mitochondria. To assess the nature of the annexin V-mitochondrial interaction, an annexin V binding activity was partially purified from placental cytosol by annexin V-affinity chromatography. Five polypeptides in the eluate appeared to be associated with annexin V, with a predominant species of 27 kDa. Antibodies to the 27 kDa polypeptide recognised mitochondria but not nucleoli. We conclude that annexin V interacts with a 27 kDa mitochondrial polypeptide that is possibly part of a larger complex.
authors.affiliation: Department of Medicine, Monash Medical School, Clive Ward Centre, Box Hill Hospital, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: C H
authors.initials: CH
authors.lastname: Bird
authors.forename: H H
authors.initials: HH
authors.lastname: Salem
authors.forename: P
authors.initials: P
authors.lastname: Bird
mesh_terms: D017304:Annexin A5 / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007163:Immunosorbent Techniques; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D010920:Placenta / Q000737:chemistry
chemical_list: D017304:Annexin A5; D002352:Carrier Proteins