title: [H+-K+-exchange and formation of H2 in E. coli mutants with defects in the H+-ATPase complex and potassium transport].
abstract: It was shown that 2H(+)-K(+)-exchange through H(+)-K(+)-pump composed of H(+)-ATPase complex F0F1 and Trk-system of potassium accumulation, H(+)-K(+)-exchange through H(+)-K(+)-antiport, composed of H(+)-channel F0 and of system with Trk-defect, and H2-production in E. coli grown in anaerobic conditions, change in mutants with defects of F0F1 and potassium transport. In unc-mutant of E. coli AN 936 with defects of c-subunit F0 (M(r) 8,4 kDa) H(+)-K(+)-exchange and H2-production disappeared. Mutants of E. coli TK 509 (2240) with defect of Trk-system not able to perform 2H(+)-K(+)-exchange, performed, however H(+)-K(+)-exchange and H2-production which can be blocked of N, N'-dicyclohexylcarbodiimide (DCCD). In mutant of E. coli Tk 509 (2242) with defects of KdpA-protein of Kdp-system of potassium accumulation, Trk A and Trk D proteins of corresponding systems of potassium accumulation, the H(+)-K(+)-exchange disappeared, while H(+)-formation and H2-production, blocked by DCCD, external osmotic pressure and absence of potassium in the medium, persisted. It is assumed that 2H(+)-K(+)-exchange, H(+)-K(+)-exchange and H2-production are connected, and the membrane-bound format-hydrogenlyase oxidizing format to H2 and CO2, directly take part in interaction with F0F1 and Trk, as well as F0 and defective Trk-system in formation of supercomplexs. It is assumed that the format-hydrogen-lyase can interact with F0F1 without the Trk-system.
authors.forename: K A
authors.initials: KA
authors.lastname: Bagramian
authors.forename: A A
authors.initials: AA
authors.lastname: Trchunian
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D009154:Mutation; D011188:Potassium / Q000378:metabolism*; D027961:Potassium-Hydrogen Antiporters; D006180:Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D027961:Potassium-Hydrogen Antiporters; D004024:Dicyclohexylcarbodiimide; D006859:Hydrogen; D006180:Proton-Translocating ATPases; D011188:Potassium
vernacular_title: H+-K+-obmen i obranozovanie H2 u mutantov E. coli s defektami po H+-ATFaznomu kompleksu i kalievomu transportu.