Low mitogenic activity of synthetic lipid A analog, 2,3-acyloxyacylgalactosamine-4-phosphate, in cultured murine splenocytes.
The mitogenicity of chemically synthesized lipid A analogs, 2,3-acyloxyacylglucosamine-4-phosphate (A-103) and 2,3-acyloxyacylgalactosamine-4-phosphate (A-113), was compared. Synthetic lipid A analogs of the disaccharide-type (506), the monosaccharide-type (A-103) suspended in RPMI-1640 medium supplemented with triethylamine, and Salmonella typhimurium LT-2 lipopolysaccharide (LPS) were capable of increasing the incorporation of [3H]thymidine into splenocytes of C57BL/6 mice at various doses ranging from 1.0 to 100 micrograms/ml. However, the mitogenic activity of A-113 at these doses was markedly weaker than the activity of the above materials. When the A-103 and A-113 were suspended in RPMI-1640 medium supplemented with ethanol, the mitogenic activity of A-113 also showed lower activity than that of A-103 in the splenocytes of C57BL/6 mice. These findings indicate that the mitogenic activity of synthetic lipid A of the monosaccharide-type is affected by a kind of composed sugar.
Shimizu
T
T
University of Shizuoka, School of Pharmaceutical Sciences, Japan.
Masuzawa
T
T
Iwamoto
Y
Y
Yanagihara
Y
Y
Sano
K
K
Ikeda
K
K
Achiwa
K
K
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D008050:Lipid A / Q000031:analogs & derivatives* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000378:metabolism; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism
D008050:Lipid A; D008070:Lipopolysaccharides; D008934:Mitogens; C083025:A 113; C083024:A 103; D013936:Thymidine