Interferon alpha induces protein kinase C-epsilon (PKC-epsilon) gene expression and a 4.7-kb PKC-epsilon-related transcript.
Protein kinases play key roles in the induction by human interferon alpha (IFN-alpha) of specific gene expression and biological activity in various human cell lines. We now report that IFN-alpha increased the 7-kb transcript for the epsilon isotype of protein kinase C (PKC-epsilon) and the cellular content of PKC-epsilon 24 and 48 hr after IFN-alpha addition (a 2-fold and 6-fold increase, respectively). Furthermore, IFN-alpha markedly induced a 4.7-kb transcript that hybridized to a PKC-epsilon-specific, but not to a PKC-eta-specific, cDNA probe. The induction of the 4.7-kb PKC-epsilon-related mRNA by IFN-alpha had the following properties reported for the classical IFN-alpha-stimulated genes: rapid kinetics of induction, high maintained levels in IFN-alpha-sensitive but not in IFN-alpha-resistant cell lines, protein synthesis-independent induction, and high sensitivity to inhibitors of protein tyrosine kinase activity. These results show that the regulation of gene expression by IFN-alpha include not only the classical IFN-alpha-stimulated genes but also the coordinated regulation of two PKC-epsilon-related transcripts that appeared to be highly relevant to the biological actions of IFN-alpha.
Wang
C
C
Department of Pathology, University of Tennessee Health Science Center, Memphis 38163.
Constantinescu
S N
SN
MacEwan
D J
DJ
Strulovici
B
B
Dekker
L V
LV
Parker
P J
PJ
Pfeffer
L M
LM
D016227:Benzoquinones; D003513:Cycloheximide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D047029:Lactams, Macrocyclic; D011493:Protein Kinase C / Q000235:genetics*; D051744:Protein Kinase C-epsilon; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins; D017828:Rifabutin / Q000031:analogs & derivatives; D013997:Time Factors; D014407:Tumor Cells, Cultured
D016227:Benzoquinones; D007370:Interferon Type I; D047029:Lactams, Macrocyclic; D011809:Quinones; D012333:RNA, Messenger; D011994:Recombinant Proteins; D017828:Rifabutin; C020754:herbimycin; D003513:Cycloheximide; D011505:Protein-Tyrosine Kinases; C497255:PRKCE protein, human; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon