Mutation-spectrum of a true abasic site in codon 12 of a c-Ha-ras gene in mammalian cells.
An abasic site is postulated to be a premutagenic lesion. We previously reported that a c-Ha-ras gene with an abasic site analogue at either the first or the second position of codon 12 induced the formation of foci by point-mutational activation of the gene [Kamiya et al. (1992) Nucleic Acids Res. 20, 4409-4415]. In this study we constructed c-Ha-ras genes with a true abasic site in codon 12. The ras genes were found to be activated in NIH3T3 cells by a mutation to A at the modified and flanking positions, and the flanking mutations were detected more frequently.
Kamiya
H
H
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Suzuki
M
M
Ohtsuka
E
E
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D003062:Codon; D005786:Gene Expression Regulation; D011905:Genes, ras / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D014162:Transfection
D003062:Codon; D009838:Oligodeoxyribonucleotides