Changes in protein kinase C isozymes in the rat hippocampus following transient hypoxia.
The effects of hypoxia on protein kinase C (PKC) isozymes (alpha, beta I, beta II, and gamma) were examined in the hippocampus from rats subjected to hypoxic conditions (5% O2 in 95% N2) for 30 min in a chamber. Western blot analysis revealed that the total amounts of PKC-alpha (-26.0% of control) and -gamma (-32.7% of control) were decreased significantly at the end of hypoxia, which was followed by the reduction of that of PKC-beta II (-23.7% of control at 7 days after hypoxia). Whereas, the PKC activities, which were measured by the incorporation of [gamma-32P] into a specific PKC substrate peptide, in both the cytosolic and the particulate fractions did not change. The reductions of PKC-gamma and -alpha at the end of hypoxia may be related to the following neuronal degeneration.
Yamaoka
Y
Y
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
Shimohama
S
S
Kimura
J
J
Fukunaga
R
R
Taniguchi
T
T
D000818:Animals; D015153:Blotting, Western; D002154:Calpain / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology*; D002534:Hypoxia, Brain / Q000201:enzymology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D007527:Isoenzymes; D011493:Protein Kinase C; D002154:Calpain