Electron microscopic localization of ribosomal DNA in rat liver nucleoli by nonisotopic in situ hybridization.
We have used postembedding nonisotopic in situ hybridization, with biotinylated rat ribosomal DNA (rDNA) as a probe and streptavidin coupled to 10-nm colloidal gold particles as the detection system, to localize rDNA sequences in rat liver nucleoli at the electron microscopic level. For comparison purposes, immunoelectron microscopy was performed for the detection of DNA. Our results indicate that ribosomal DNA sequences are enriched in the dense fibrillar component of the rat liver nucleolus. These data are discussed in relation to the putative site(s) for transcription of ribosomal genes.
Jiménez-García
L F
LF
Departamento de Biología, Facultad de Ciencias, U.N.A.M., México D. F.
Segura-Valdez
M L
ML
Ochs
R L
RL
Echeverría
O M
OM
Vázquez-Nin
G H
GH
Busch
H
H
D000818:Animals; D001426:Bacterial Proteins; D002466:Cell Nucleolus / Q000737:chemistry* / Q000648:ultrastructure*; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000032:analysis* / Q000648:ultrastructure*; D004173:Dipodomys; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D017403:In Situ Hybridization; D007668:Kidney / Q000166:cytology / Q000648:ultrastructure; D008099:Liver / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D019809:Streptavidin
D001426:Bacterial Proteins; D015342:DNA Probes; D004275:DNA, Ribosomal; D019809:Streptavidin