title: [Preparation of hybrid proteins consisting of human interleukin-2 and the cytotoxic A-subunit of Shigella toxin].
abstract: Recombinant plasmids providing the synthesis of chimeric proteins consisting of amino acid sequences of human interleukin-2 (IL-2) and Shiga toxin cytotoxic A-subunit (ILA and AIL chimeric toxins) were constructed. The ILA and AIL chimeric toxins were shown to inhibit protein synthesis in the rabbit reticulocytes cell-free system. These chimeric toxins displayed two opposite activities of the constituent parts of their molecules on T-lymphocytes from the peripheral blood of healthy volunteers. Hybrid protein AIL (approximately 10(-6) g/ml) has caused the most significant depression of T-lymphoblast proliferation.
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: A N
authors.initials: AN
authors.lastname: Siniakov
authors.forename: S G
authors.initials: SG
authors.lastname: Pozdniakov
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Kozlov
authors.forename: L V
authors.initials: LV
authors.lastname: Sakhno
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Leplina
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Nomokonova
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry*; D001483:Base Sequence; D004274:DNA, Recombinant; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry*; D008969:Molecular Sequence Data; D010957:Plasmids; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D022603:Shiga Toxins
chemical_list: D001427:Bacterial Toxins; D004274:DNA, Recombinant; D007376:Interleukin-2; D011500:Protein Synthesis Inhibitors; D011993:Recombinant Fusion Proteins; D022603:Shiga Toxins
vernacular_title: Poluchenie gibridnykh belkov, sostoiashchikh iz interleuĭkina-2 cheloveka i tsitotoksicheskoĭ A-sub''edinitsy toksina shigelly.