title: Role of platelet-derived growth factor in development of in vitro matured and in vitro fertilized bovine embryos.
abstract: This experiment was designed to determine whether the stimulatory effects of bovine oviductal epithelial cells (BOEC) on development of early bovine embryos are due to platelet-derived growth factor (PDGF). Four hundred and twenty five 8-cell bovine embryos derived from in vitro maturation and in vitro fertilization procedures were equally and randomly allotted to one of the following culture treatment groups: control medium alone (Menezo's B2 medium; MB2), MB2 with 1 ng PDGF ml-1 (PDGF), 1 ng PDGF ml-1 and 10 micrograms anti-PDGF antibody ml-1 (PDGF + Ab), BOEC or BOEC and 10 micrograms anti-PDGF antibody ml-1 (BOEC + Ab). All embryos were cultured in 100 microliters of serum-free MB2 medium supplemented with 2 mg fatty-acid-free bovine serum albumin ml-1. Embryos for all treatment groups were incubated at 39 degrees C and 5% CO2 in humidified air in groups of five embryos per well in 96-well culture plates until 7 days after in vitro insemination. A higher proportion of embryos developed to > 8-cell and to the morula stage following culture with PDGF, BOEC or BOEC+Ab than with MB2 alone. Incubation of PDGF and BOEC-treated embryos with anti-PDGF reduced development to the morula and blastocyst stages. However, anti-PDGF did not completely inhibit blastocyst development when added to BOEC. In addition, embryos incubated with BOEC and anti-PDGF contained a reduced number of inner cell mass cells compared with embryos incubated with BOEC alone. These results indicate that PDGF provides a developmental stimulus similar to BOEC for bovine embryos at the fourth cell cycle.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Science, Louisiana State University, Baton Rouge 70803.
authors.forename: J K
authors.initials: JK
authors.lastname: Thibodeaux
authors.forename: R P
authors.initials: RP
authors.lastname: Del Vecchio
authors.forename: W
authors.initials: W
authors.lastname: Hansel
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000502:physiology*; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology*
chemical_list: D010982:Platelet-Derived Growth Factor