title: Characterization of adhesion of "resting" and stimulated platelets to fibrinogen and its fragments.
abstract: Adhesion of resting and stimulated platelets to immobilized fibrinogen (Fg) was characterized using various forms of Fg, receptor peptide mimics, and antibodies to glycoprotein (GP) IIb/IIIa and Fg. Resting platelets adhered to Fg, but to less than half the extent of the same platelets stimulated with epinephrine/ADP. The adhesion of resting and stimulated platelets to Fg was inhibited by a receptor peptide mimic (G13, a peptide corresponding to residues 300-312 of GPIIb), anti-GPIIb/IIIa antibodies, and a monoclonal antibody (4A5) against the carboxyl terminus of the gamma chain of Fg. The results presented here demonstrate that the alpha chain RGD platelet recognition sites are not required to mediate the adhesion of either stimulated or resting platelets to immobilized Fg. Although stimulated platelets can adhere extensively to monomeric Fg containing one functional gamma chain, resting platelets require bivalent Fg containing two functional gamma chains to mediate irreversible adhesion to Fg.
authors.affiliation: Department of Biology, Memphis State University, TN 38152.
authors.forename: T K
authors.initials: TK
authors.lastname: Gartner
authors.forename: D L
authors.initials: DL
authors.lastname: Amrani
authors.forename: J M
authors.initials: JM
authors.lastname: Derrick
authors.forename: N E
authors.initials: NE
authors.lastname: Kirschbaum
authors.forename: G R
authors.initials: GR
authors.lastname: Matsueda
authors.forename: D B
authors.initials: DB
authors.lastname: Taylor
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D005340:Fibrinogen / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000097:blood*; D010973:Platelet Adhesiveness / Q000187:drug effects* / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D012016:Reference Values
chemical_list: D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D010980:Platelet Membrane Glycoproteins; D005340:Fibrinogen