Application of high-performance capillary electrophoresis to the analysis of H1 histones.
High-performance capillary electrophoresis for the separation of rat testis H1 histone variants and their phosphorylated modifications is described. The influence of buffer pH, hydroxypropylmethyl cellulose, and buffer concentration has been investigated. Under optimized conditions (500 mM phosphate buffer, pH 2, 0.03% hydroxypropylmethyl cellulose) using an uncoated capillary, eight H1 histone subfractions, including two H1(0) histones and H1t and their phosphorylated modifications, are resolved. Application of capillary electrophoresis to the separation of H1 histones provides an important new alternative to high-performance liquid chromatography (HPLC) and traditional gel electrophoresis.
Lindner
H
H
Institute of Medical Chemistry and Biochemistry, Innsbruck, Austria.
Wurm
M
M
Dirschlmayer
A
A
Sarg
B
B
Helliger
W
W
D002021:Buffers; D005591:Chemical Fractionation; D004586:Electrophoresis / Q000379:methods*; D006657:Histones / Q000032:analysis*; D009994:Osmolar Concentration; D010710:Phosphates; D010766:Phosphorylation; D013997:Time Factors
D002021:Buffers; D006657:Histones; D010710:Phosphates