[Iodination and binding characteristics of 125I-d(CH2)5 [Tyr(Me)2, Tyr(NH2)9] AVP].
With iodinated vasopressin analogue, d(CH2)5[Tyr(Me)2, Tyr(NH2)9] AVP, at position 9, followed by purification by HPLC (specific activity 473-543Ci/mmol), a specific binding was observed in the rat liver plasma membrane fraction. Scatchard analysis indicated a single class of high-affinity binding sites with a Kd of 0.23nM and Bmax of 142fmol/mg protein. V2-agonist, DDAVP, did not displace 125I-vasopressin analogue. These results suggest that 125I-d(CH2)5[Tyr(Me)2, Tyr(NH2)9] AVP with a high specific activity is a useful tool to investigate V1-receptors.
Inaba
M
M
Fourth Department of Medicine, Saitama Medical School.
Katayama
S
S
Akabane
S
S
Maruno
Y
Y
Itabashi
A
A
Ishii
J
J
D000818:Animals; D001127:Arginine Vasopressin / Q000031:analogs & derivatives* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003894:Deamino Arginine Vasopressin / Q000378:metabolism; D007455:Iodine / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017483:Receptors, Vasopressin / Q000378:metabolism
D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; C055245:argipressin, beta mercapto-beta,beta-cyclopentamethylenepropionic acid(1)-O-methyl-Tyr(2)-TyrNH2(9)-; D007455:Iodine; D003894:Deamino Arginine Vasopressin