title: Measurement of carboxyhemoglobin and total hemoglobin by five specialized spectrophotometers (CO-oximeters) in comparison with reference methods.
abstract: We measured total hemoglobin (CtHb) and carboxyhemoglobin (COHb) in 100 patients' blood samples by using five specialized spectrophotometers (CO-oximeters)--IL 482 CO-Oximeter, Corning 2500 CO-oximeter, Radiometer OSM 3 Hemoximeter, Corning 270 CO-oximeter, and the AVL 912 CO-Oxylite--and compared the results with those obtained with the manual cyanmethemoglobin method and a gas-chromatographic (GC) method, respectively. For the CtHb measurements, the differences between the cyanmethemoglobin method and the CO-oximeters were not clinically important for any model. For the blood COHb measurements, the direction of the bias relative to GC was dependent on COHb concentration. In general, the CO-oximeters underestimated COHb concentration for COHb > 2.5% of total hemoglobin but overestimated COHb concentration for COHb < or = 2.5%. We conclude that all five CO-oximeters compared favorably with the reference methods for CtHb and for high concentrations of COHb. However, the inaccuracy of CO-oximeters for low-concentration (< or = 2.5%) COHb measurements may make these instruments unsuitable for some applications.
authors.affiliation: Department of Pulmonary Physiology, Stanford University Hospital, CA 94305.
authors.forename: J J
authors.initials: JJ
authors.lastname: Mahoney
authors.forename: H J
authors.initials: HJ
authors.lastname: Vreman
authors.forename: D K
authors.initials: DK
authors.lastname: Stevenson
authors.forename: A L
authors.initials: AL
authors.lastname: Van Kessel
mesh_terms: D002263:Carboxyhemoglobin / Q000032:analysis*; D002849:Chromatography, Gas; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008706:Methemoglobin / Q000031:analogs & derivatives / Q000032:analysis; D010092:Oximetry / Q000295:instrumentation; D011786:Quality Control; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods
chemical_list: D006454:Hemoglobins; C007169:cyanomethemoglobin; D008706:Methemoglobin; D002263:Carboxyhemoglobin