title: Inhibitory action of azelastine on cytokine production from human peripheral blood leukocytes in vitro.
abstract: Effects of azelastine (AZ) on human peripheral blood leukocytes (PBL) in culture were examined. Addition of 10.0 micrograms/ml of AZ resulted in a marked inhibition of PBL blastic activity, but lower concentrations (1.0 and 0.5 microgram/ml) had no demonstrable suppressive effects on the activation. Interleukin (IL)-2, IL-3 and IL-4 production from PBL in response to Concanavalin A stimulation was also strongly suppressed when the cells were cultured in the presence of AZ. This suppression was observed even when lower concentrations (1.0 and 0.5 microgram/ml) of AZ were added to cell cultures.
authors.affiliation: First Department of Internal Medicine, School of Medicine, Showa University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Konno
authors.forename: M
authors.initials: M
authors.lastname: Adachi
authors.forename: K
authors.initials: K
authors.lastname: Asano
authors.forename: S
authors.initials: S
authors.lastname: Matsuo
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003208:Concanavalin A / Q000037:antagonists & inhibitors; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D010793:Phthalazines / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D010793:Phthalazines; D003208:Concanavalin A; C020976:azelastine