title: Actin filaments in the ruminal epithelium of cattle.
abstract: Phalloidin and immunogold labeling were used to localize actin filaments in the ruminal pilar epithelium of adult cattle on the light and electron microscopic level. Incubation with rhodaminyl-phalloidin permitted an overview of general actin distribution pattern. Distinct subcellular localization of actin was revealed with the biotin-streptavidin bridge technique in a postembedding procedure. Superior fixation of membranes and filaments was achieved with tannic acid-glutaraldehyde. Surface-etching prior to immunoincubation ensured restoration of actin immunoreactivity. Patterns of distribution in non-keratinized and keratinized epithelial cells as well as in pericytes and endothelial cells point to cell-specific cytoskeletal and motile functions of actin filaments.
authors.affiliation: Department of Veterinary Anatomy, Histology and Embryology, University of Giessen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kressin
authors.forename: B
authors.initials: B
authors.lastname: Koob
authors.forename: B
authors.initials: B
authors.lastname: Schnorr
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D002417:Cattle / Q000033:anatomy & histology*; D004848:Epithelium / Q000033:anatomy & histology / Q000737:chemistry; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D012417:Rumen / Q000033:anatomy & histology* / Q000737:chemistry
chemical_list: D000199:Actins