Further evidence for the existence of endogenous serotonin uptake inhibitors and their purification by calmodulin affinity chromatography.
Experimental evidence suggests the occurrence of endogenous antidepressant-like compounds in the brain, blood plasma, and urine. After extensive purification by calmodulin-sepharose affinity chromatography, and further purification of the urine-derived sample by exclusion chromatography, at least three distinctive fractions have been identified. These fractions effectively inhibited serotonin uptake, imipramine, and/or paroxetine binding, and they apparently contained some compounds that were recognized by the anti-imipramine or anti-paroxetine antibodies. Their identification may have significant implications for studies of affective illness.
Chudzik
J
J
Royal Ottawa Hospital, Department of Neuropharmacology, Ontario, Canada.
Strijewski
A
A
Tang
S W
SW
D000328:Adult; D000906:Antibodies / Q000032:analysis; D001685:Biological Factors / Q000032:analysis / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D003866:Depressive Disorder / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007099:Imipramine / Q000276:immunology / Q000378:metabolism / Q000627:therapeutic use; D008297:Male; D017374:Paroxetine / Q000276:immunology / Q000378:metabolism / Q000627:therapeutic use; D011863:Radioimmunoassay; D012701:Serotonin / Q000276:immunology / Q000378:metabolism* / Q000627:therapeutic use; D017367:Selective Serotonin Reuptake Inhibitors; D013329:Structure-Activity Relationship
D000906:Antibodies; D001685:Biological Factors; D002147:Calmodulin; D007099:Imipramine; D017374:Paroxetine; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors