title: Packaging zinc, fibrinogen, and factor XIII in platelet alpha-granules.
abstract: Zinc(II) accumulated by platelets has profound effects on platelet activity. This study is focused on the distribution of Zn(II) between human platelet subcellular compartments. After incubation with 86Rb+ and platelet lysis, the organelles were separated by sucrose density gradient centrifugation. Fibrinogen served as a marker for alpha-granules. 86Rb+ and factor XIII served as markers for the cytoplasmic fractions. Zn(II) was found to be distributed between the cytoplasm and the alpha-granules, with variations between different individual units. The total platelet Zn concentration and its relative subcellular distribution were dependent on its extracellular level. Incubation of platelets with 100 microM Zn(II) resulted in a twofold increase of its level in the cytoplasm and by one order of magnitude in the alpha-granules. In addition to the anticipated factor XIII activity in the cytoplasmic pool fraction, we found thrombin-inducible factor XIII activity within the alpha-granules. Immunoblotting confirmed the presence of both the a and b subunits of plasma factor XIII (a2b2 form) in the alpha-granules. As fibrinogen is not synthesized in the platelet, we propose that by virtue of their mutual binding, fibrinogen, Zn(II) and plasma factor XIII-a2b2 are simultaneously taken up into the alpha-granules by endocytosis, presumably through the vehicle of the GPIIb/IIIa fibrinogen receptor. A rationale for co-packaging these components within the alpha-granules is that Zn(II) inhibits factor XIII activity and thereby prevents the premature cross-linking of the concentrated fibrinogen prior to platelet activation and secretion. By contrast, cytoplasmic Zn(II) may increase platelet responsiveness to agonists due to its interaction with cytoplasmic modulators of platelet activity.
authors.affiliation: New York Blood Center, New York 10021.
authors.forename: G
authors.initials: G
authors.lastname: Marx
authors.forename: G
authors.initials: G
authors.lastname: Korner
authors.forename: X
authors.initials: X
authors.lastname: Mou
authors.forename: R
authors.initials: R
authors.lastname: Gorodetsky
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005176:Factor XIII / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D012413:Rubidium / Q000097:blood; D012414:Rubidium Radioisotopes; D013347:Subcellular Fractions / Q000378:metabolism; D015032:Zinc / Q000097:blood*
chemical_list: D012414:Rubidium Radioisotopes; D005340:Fibrinogen; D005176:Factor XIII; D015032:Zinc; D012413:Rubidium