[Inhibitors of nucleic acid synthesis as a means of identifying the forms of DNA-dependent DNA polymerases in Acholeplasma laidlawii PG-8 and of determining their functions].
Antibiotics, inhibitors of nucleic acids' synthesis from the group of chromomycins (olivomycin of sodium salt), anthracyclines (carminomycin and doxorubicin) and streptonigrin (bruneomycin) have been studied for their effect on DNA synthesis in vitro performed by DNA polymerases (1st and 2nd forms) of Acholeplasma laidlawii PG-8. It has been stated that olivomycin inhibits the function of both the first and second forms of DNA polymerases in proportion to an increase of the antibiotic concentration in the medium. Carminomycin in the concentration of about 1 microgram/ml almost completely inhibited the activity of both DNA polymerases. However, doxorubicin also belonging to the group of anthracyclins completely inhibited the activity of the first form of DNA polymerase in the concentration of 1 microgram/ml and practically has no effect in the concentration up to 100 micrograms/ml on the activity of the second form possessing 3'-->5'-function. Streptonigrin also proved to be suitable for differentiate the forms of DNA polymerases and to determine their functions. The first form of DNA polymerase with 5'-->3'-polymerase and exonuclease functions was not sensitive by this antibiotic in the concentration of 1000 micrograms/ml, while the activity of the second form of DNA polymerase with 3'-->5'-exonuclease functions was fully inhibited by this concentration of the antibiotic in the medium. The combination of doxorhubicin and streptonigrin in the medium can be used to determine the form of DNA polymerases and to identify their 5'-->3'- or 3'-->5'-exonuclease function and for selectivity inhibition of the function of one or another DNA polymerase in the medium.
Skripal'
I G
IG
Bezuglyĭ
S V
SV
Babichev
V V
VV
D000128:Acholeplasma laidlawii / Q000187:drug effects* / Q000201:enzymology; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002865:Chromomycins / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000187:drug effects*; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D013308:Streptonigrin / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D000903:Antibiotics, Antineoplastic; D002865:Chromomycins; D004269:DNA, Bacterial; D013308:Streptonigrin; D004259:DNA-Directed DNA Polymerase
Ingibitory sinteza nukleinovykh kislot kak sredstvo identifikatsii form DNK-zavisimykh DNK-polimeraz Acholeplasma laidlawii PG-8 i opredeleniia ikh funktsiĭ.