title: Mutations in beta-actin: influence on polymer formation and on interactions with myosin and profilin.
abstract: Two beta-actin mutants, one with proline 38 replaced with alanine (P38A) and the other with cysteine-374 replaced with serine (C374S), as well as the wild-type beta-actin, were expressed in the yeast, S. cerevisiae, purified to homogeneity, and analyzed in vitro for polymerizability and interaction with DNase I, myosin, and profilin. Both mutations interfered with the polymerization of the actin, and with its interaction with myosin. The C374S mutation had the most pronounced effect; it reduced the polymerizability of the actin, abolished its binding to profilin, and filaments containing this mutation moved at reduced rates in the in vitro 'motility assay'. The ATPase activity measured in solutions containing myosin subfragment 1 was similar for both the mutant and wild-type actins.
authors.affiliation: Department of Development Biology, Uppsala University, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Aspenström
authors.forename: C E
authors.initials: CE
authors.lastname: Schutt
authors.forename: U
authors.initials: U
authors.lastname: Lindberg
authors.forename: R
authors.initials: R
authors.lastname: Karlsson
mesh_terms: D000199:Actins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D003285:Contractile Proteins; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors; D004533:Egtazic Acid / Q000494:pharmacology; D006801:Humans; D015636:Magnesium Chloride / Q000494:pharmacology; D008840:Microfilament Proteins / Q000378:metabolism*; D009154:Mutation; D009218:Myosins / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D051304:Profilins; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013329:Structure-Activity Relationship; D014783:Viscosity
chemical_list: D000199:Actins; D003285:Contractile Proteins; D008840:Microfilament Proteins; C495738:PFN1 protein, human; D011108:Polymers; D051304:Profilins; D011994:Recombinant Proteins; D015636:Magnesium Chloride; D004533:Egtazic Acid; D011189:Potassium Chloride; D003850:Deoxyribonuclease I; D009218:Myosins