Cloning and sequencing of a soybean nuclear gene coding for a chloroplast translation elongation factor EF-G.
A plant nuclear gene coding for a chloroplast specific translation elongation factor EF-G (cEF-G) was cloned and sequenced for the first time. We screened two partial soybean genomic libraries with a short PCR amplified pea DNA probe constructed according to the N-terminal peptide sequence of pea chloroplast EF-G. The gene is three times split, codes for a chloroplast type transit peptide and a protein very similar to bacterial translation elongation factor EF-G. The gene is expressed as evidenced by Northern hybridisations.
Hernández Torres
J
J
Laboratoire de Biochimie végétale, Université de Neuchâtel, Switzerland.
Breitenberger
C A
CA
Spielmann
A
A
Stutz
E
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000235:genetics*
D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D010940:Plant Proteins