Complete nucleotide sequence of HTLV-II isolate NRA: comparison of envelope sequence variation of HTLV-II isolates from U.S. blood donors and U.S. and Italian i.v. drug users.
The entire nucleotide sequence of human T-cell lymphotropic virus type II (HTLV-II) from a previously described isolate of patient NRA (HTLV-IINRA) was determined. Clones encoding the 5' LTR and gag, pol, env and tax/rex open reading frames were subcloned and sequenced on both strands. The provirus consisted of 8957 nucleotides and showed 95.2% homology with the HTLV-IIMo prototype at the nucleotide level. Less than 5% amino acid variation between HTLV-IINRA and HTLV-IIMo was observed for coding regions. Although isolate HTLV-IINRA had an additional 25 amino acids at the 3' end of tax/rex, this region was 96% homologous with the 5' end of HTLV-IIMo 3' LTR. To further investigate HTLV-II variability, a portion of the env gp46 gene derived from 9 HTLV-II infected persons was amplified by polymerase chain reaction and sequenced. Sequence was obtained for 320 nucleotides corresponding to HTLV-IIMo positions 5291 to 5610. Isolates similar to the HTLV-IIMo and HTLV-IINRA prototypes were identified, and sequences were highly conserved.
Lee
H
H
Diagnostics Division, Abbott Laboratories, North Chicago, Illinois 60064.
Idler
K B
KB
Swanson
P
P
Aparicio
J J
JJ
Chin
K K
KK
Lax
J P
JP
Nguyen
M
M
Mann
T
T
Leckie
G
G
Zanetti
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D001782:Blood Donors; D002460:Cell Line; D003430:Cross-Sectional Studies; D004279:DNA, Viral; D015686:Gene Products, env / Q000235:genetics*; D014644:Genetic Variation; D016679:Genome, Viral; D015491:HTLV-II Infections / Q000453:epidemiology / Q000382:microbiology*; D015367:Human T-lymphotropic virus 2 / Q000145:classification / Q000235:genetics*; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015819:Substance Abuse, Intravenous; D014481:United States / Q000453:epidemiology; D054299:env Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015686:Gene Products, env; D015682:Retroviridae Proteins, Oncogenic; D054299:env Gene Products, Human Immunodeficiency Virus; C069568:gp46 protein, Human immunodeficiency virus 2