title: Chromosome mapping in Alcaligenes eutrophus CH34.
abstract: Mutants and mobilizing plasmids were developed as genetic tools in Alcaligenes eutrophus CH34. In order to map the chromosome, spontaneous and ethyl methane sulphonate (EMS)-induced mutants (mostly auxotrophs) were isolated. Another source of mutants was provided by the phenomenon of temperature-induced mortality and mutagenesis that is observed at 37 degrees C and is characteristic of many metallotolerant strains of A. eutrophus. Plasmid pULB113 (RP4::miniMu) was used to map the available mutations. Twenty-five loci were ordered in a circular map. pMOL50, a rearranged derivative of plasmid pMOL28, which was obtained in a survivor at 37 degrees C and displayed chromosome mobilizing activity (Cma+), was also used to mobilize chromosomal markers: resulting linkages were stronger than with pULB113, allowing confirmation of the circularity of the A. eutrophus CH34 chromosome with a small number of crosses.
authors.affiliation: Laboratoire de Génétique et Biotechnologie, Centre d'Etudes de l'Energie Nucléaire and Vlaamse Instelling voor Technologisch Onderzoek, SCK/CEN-VITO, Mol, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Sadouk
authors.forename: M
authors.initials: M
authors.lastname: Mergeay
mesh_terms: D000421:Alcaligenes / Q000187:drug effects / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D008040:Genetic Linkage; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D010957:Plasmids; D013696:Temperature
chemical_list: D008769:Methylnitronitrosoguanidine; D005020:Ethyl Methanesulfonate