Isolation and identification of a novel peptide from the accessory sex gland of the female house fly, Musca domestica.
A peptide of novel structure was isolated from accessory glands of the female house fly, Musca domestica by molecular-weight limit centrifugal ultrafiltration and reversed-phase HPLC. After amino acid and protein sequence analyses, the structure of this peptide was confirmed by chemical synthesis and plasma desorption mass spectral analysis to be Leu-Leu-Asn-Ala-Leu-Pro-Leu-Asp-Ala-Leu-Ser-Ser-Leu-Thr-Gly-NH2. The accessory gland peptide stimulated contraction of the house fly oviduct at concentrations of 10(-7) M and above.
Wagner
R M
RM
United States Department of Agriculture, Livestock Insects Laboratory, Livestock, Beltsville, MD.
Woods
C W
CW
Hayes
J A
JA
Kochansky
J P
JP
Hill
J C
JC
Fraser
B A
BA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005260:Female; D005836:Genitalia, Female / Q000737:chemistry*; D006793:Houseflies / Q000502:physiology*; D007301:Insect Hormones / Q000302:isolation & purification* / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010057:Oviducts / Q000187:drug effects*; D017421:Sequence Analysis
D000596:Amino Acids; D007301:Insect Hormones; C082629:accessory sex gland peptide, Musca domestica