title: Culture of human preovulatory granulosa cells: effect of extracellular matrix on steroidogenesis.
abstract: Human luteal granulosa cells, harvested from preovulatory follicles during in vitro fertilization attempts, were cultured in a serum-precoated substratum ('serum cells') or on a collagen matrix ('collagen cells'). Concerning the 'serum cell' model, E2 secretion was very low in the absence of androgen; when androstenedione was added to the culture medium, cells secreted 180 +/- 52 pmol/ml/24 h of estradiol, 440 +/- 78 pmol/ml/24 h of testosterone and lower quantities of estrone and estriol. Follicle stimulating hormone induced a significant increase in estradiol and estriol, while the secretion of the other steroids was not altered. The secretion of progesterone was 3.15 +/- 1 nmol/ml/24 h and significantly enhanced by luteinizing hormone (+ 95%; P < 0.01). The secretions of 17 alpha-hydroxyprogesterone and 20 alpha-dihydroprogesterone were low and not modified by luteinizing hormone. 'Collagen cells', in basal conditions, showed an increased secretion of estradiol (+ 50%, P < 0.05), became rounded and were less responsive to gonadotropins when compared with 'serum cells'. Thus, the use of a collagen matrix, similarly to gonadotropins, stimulated granulosa cell steroidogenesis in relation to modifications of cell shape. The higher responsiveness of serum cells to gonadotropins makes this model more suitable for physiological and pharmacological studies than the collagen one.
authors.affiliation: INSERM CJF 89-08 and IVF Laboratory, CHR La Grave, Toulouse, France.
authors.forename: I
authors.initials: I
authors.lastname: Bussenot
authors.forename: G
authors.initials: G
authors.lastname: Ferre
authors.forename: C
authors.initials: C
authors.lastname: Azoulay-Barjonet
authors.forename: C
authors.initials: C
authors.lastname: Murgo
authors.forename: G
authors.initials: G
authors.lastname: Vieitez
authors.forename: J
authors.initials: J
authors.lastname: Parinaud
mesh_terms: D001790:Blood Physiological Phenomena; D048429:Cell Size / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology; D004958:Estradiol / Q000378:metabolism*; D005109:Extracellular Matrix / Q000502:physiology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D005498:Follicular Phase / Q000502:physiology*; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D010969:Plastics; D011372:Progestins / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D010969:Plastics; D011372:Progestins; D013256:Steroids; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D003094:Collagen