Properdin, a positive regulator of complement activation, is expressed in human T cell lines and peripheral blood T cells.
Properdin plays an important regulatory role in the activation of the complement system. Here we report the biosynthesis of properdin in four different human T cell lines and in T cells purified from peripheral blood. Cell sorting experiments, in conjunction with Northern blotting, showed that both CD4- and CD8-bearing populations of T cells have the potential to synthesize properdin. The functional activity of properdin secreted from these T cell lines was determined in a hemolytic assay. In view of the numerous ways in which complement activation may influence cellular immune response, the present results indicate, for the first time, a possible interaction between the complement and the T cell system.
Schwaeble
W
W
I. Medical Clinic, University of Mainz, Germany.
Dippold
W G
WG
Schäfer
M K
MK
Pohla
H
H
Jonas
D
D
Luttig
B
B
Weihe
E
E
Huemer
H P
HP
Dierich
M P
MP
Reid
K B
KB
D000328:Adult; D001483:Base Sequence; D015152:Blotting, Northern; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002460:Cell Line; D003167:Complement Activation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011414:Properdin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D012333:RNA, Messenger; D011414:Properdin