Tri-n-butyltin chloride promotes morphological transformation and induces proliferin expression in C3H10T1/2 cells.
Transcripts from the murine gene family proliferin, which are increased by a wide assortment of chemical promoters of C3H10T1/2 cell morphological transformation, were shown to be induced by tri-n-butyltin chloride at concentrations above 50 nM. Two-stage transformation assays, with 3-methylcholanthrene as inducer and tri-n-butyltin chloride as promoter, were performed to determine if promotion of morphological transformation and proliferin induction were properties shared by this compound. Tri-n-butyltin chloride synergistically enhanced focus formation at concentrations ranging from 20 to 75 nM. Di-n-butyltin dichloride, n-butyltin trichloride and tin (II) chloride, but not tin (IV) chloride, were also effective inducers of proliferin. Changes in patterns of TPA-inducible, secreted proteins, including those likely to be proliferin, were detected following organotin treatment of confluent monolayers. Tri-n-butyltin chloride resembles other agents active as promoters in C3H10T1/2 two-stage transformation assays by possessing an ability to induce proliferin expression.
Parfett
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Mutagenesis Section, Environmental Health Directorate, Health and Welfare Canada, Tunney's Pasture, Ottawa, Ontario.
Pilon
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D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008809:Mice, Inbred C3H; D009947:Organotin Compounds / Q000494:pharmacology; D011388:Prolactin; D012333:RNA, Messenger / Q000032:analysis; D013462:Sulfur Radioisotopes; D014220:Trialkyltin Compounds / Q000494:pharmacology*
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D009947:Organotin Compounds; C000634106:Prl2c2 protein, mouse; D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D014220:Trialkyltin Compounds; C011559:tributyltin; D011388:Prolactin; C048581:monotributyltin trichloride; C020735:dibutyldichlorotin