title: A lipid-conjugated immunostimulating complex subunit vaccine against Salmonella infection in turkeys.
abstract: Immunostaining complexes (ISCOMs) are multimeric particles and have been used successfully for presentation of membrane proteins. In this study, outer-membrane proteins (OMPs) from Salmonella heidelberg were incorporated into lipid-conjugated ISCOM particles and evaluated for their use in a vaccine for turkeys against homologous and heterologous Salmonella challenge. Two types of lipid-conjugated ISCOMs were examined: ISCOM-phospholipid and ISCOM-sphingolipid preparations. The turkeys were challenged with one of the three Salmonella serotypes: S. heidelberg, S. reading, or S. enteritidis. The turkeys were monitored for clinical signs, shedding pattern post-challenge, and clearance of the challenge Salmonella from selected internal organs. Vaccines containing OMP with either lipid-conjugated ISCOM preparation produced significantly greater (P < 0.01) immune response than OMP alone. Cloacal swabs from turkeys given OMP along with ISCOM-phospholipid and challenged with a homologous serotype were completely negative for Salmonella. A certain degree of cross-protection against heterologous Salmonella was afforded by both OMP-ISCOM vaccines. The isolation rate of Salmonella from internal organs was significantly lower (P < 0.0001) in vaccinated turkeys than in unvaccinated controls.
authors.affiliation: Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
authors.forename: S D
authors.initials: SD
authors.lastname: Charles
authors.forename: K V
authors.initials: KV
authors.lastname: Nagaraja
authors.forename: V
authors.initials: V
authors.lastname: Sivanandan
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage* / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D016882:ISCOMs / Q000008:administration & dosage* / Q000276:immunology; D008854:Microscopy, Electron; D011201:Poultry Diseases / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D012475:Salmonella / Q000276:immunology* / Q000302:isolation & purification; D012481:Salmonella Infections, Animal / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014422:Turkeys / Q000276:immunology* / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D016882:ISCOMs