Simultaneous analysis of cell surface antigens and DNA content by flow cytometry.
Cells obtained from the blood or bone marrow of patients with haematological malignancies can both be stained with fluorescent labelled monoclonal antibodies to determine an immunophenotype and permeabilized with 30% ethanol then stained with propidium iodide for simultaneous DNA analysis. In the technique described here, no evidence of selective depletion of the malignant cell population was demonstrated and decreases in the mean fluorescence intensity of the surface markers were insufficient to affect the sensitivity of flow cytometric analysis. The combined method is simple and robust enough to allow incorporation of DNA analysis into routine immunophenotyping of leukaemia and lymphoma cells.
Howe
D J
DJ
Department of Haematological Medicine, Taunton and Somerset Hospital, Somerset, UK.
Hopkins
J A
JA
Johnson
S A
SA
Phillips
M J
MJ
D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis*; D000431:Ethanol; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000276:immunology*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology*; D013997:Time Factors
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D004273:DNA, Neoplasm; D000431:Ethanol