title: Characterization of the pyruvate kinase-encoding gene (pki1) of Trichoderma reesei.
abstract: The pyruvate kinase-encoding gene (pki1) from Trichoderma reesei was isolated by hybridization to the corresponding Aspergillus nidulans pkiA gene. The 1614-bp nucleotide (nt) sequence of the cloned gene codes for a 538-amino-acid protein. The coding sequence contains a single intron of 246 nt at a position identical to that of intron E in the A. nidulans gene. The PKI protein shows extensive homology to the PKIs of A. nidulans and A. niger (67%) and Saccharomyces cerevisiae (59%). The 5' non-coding sequence contains a number of motifs typical for yeast glycolytic genes, but so far only rarely found in filamentous fungi.
authors.affiliation: Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Schindler
authors.forename: R L
authors.initials: RL
authors.lastname: Mach
authors.forename: S K
authors.initials: SK
authors.lastname: Vollenhofer
authors.forename: R
authors.initials: R
authors.lastname: Hodits
authors.forename: F
authors.initials: F
authors.lastname: Gruber
authors.forename: J
authors.initials: J
authors.lastname: Visser
authors.forename: L
authors.initials: L
authors.lastname: De Graaff
authors.forename: C P
authors.initials: CP
authors.lastname: Kubicek
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005800:Genes, Fungal; D006019:Glycolysis / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D011770:Pyruvate Kinase / Q000235:genetics*; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D011770:Pyruvate Kinase