PC-766B, a new macrolide antibiotic produced by Nocardia brasiliensis. II. Isolation, physico-chemical properties and structure elucidation.
A new macrolide antibiotic, PC-766B, was isolated from the cells of Nocardia brasiliensis SC-4710 by acetone extraction, and purified by gel filtration, silica gel chromatography, HPLC and TLC. The structure of PC-766B was determined by NMR spectral analysis to be a new class of the hygrolidin family antibiotics. PC-766B had a 16-membered macrocyclic lactone ring, a 6-membered hemiketal ring and a 2-deoxy-D-rhamnose moiety. DL-alpha-Tocopherol, known as an antioxidant agent, significantly improved the stability of PC-766B and prevented the decomposition of PC-766B during the storage of the antibiotic.
Kumagai
K
K
Biotechnology Laboratory, Takarazuka Research Center, Sumitomo Chemical Co., Ltd., Hyogo, Japan.
Fukui
A
A
Tanaka
S
S
Ikemoto
M
M
Moriguchi
K
K
Nabeshima
S
S
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D018942:Macrolides; D015394:Molecular Structure; D009615:Nocardia / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018942:Macrolides; C082791:PC 766B