Different chemo- and endocrino-sensitivity of MCF-7 cells with or without estradiol supplement in vitro.
The sensitivity of MCF-7 cells to tamoxifen (TAM) and mitomycin C (MMC) was assessed in rapidly and slowly growing cells with or without estradiol supplementation, respectively. The growth of MCF-7 was inhibited by MMC in a concentration-dependent manner with or without estradiol (E2) supplementation. Preincubation with MMC suppressed subsequent E2 stimulated growth of MCF-7. TAM inhibited the growth of MCF-7 supplemented with E2 and preincubation with TAM prevented subsequent E2 stimulated growth of MCF-7. However, TAM did not inhibit the growth of MCF-7 cells in E2 free medium. These results suggested that MMC may be more effective than TAM on breast cancer cells in the dormant or slow-growth phase.
Tanino
H
H
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Kubota
T
T
Saikawa
Y
Y
Kuo
T H
TH
Takeuchi
T
T
Kase
S
S
Furukawa
T
T
Kitajima
M
M
Sakurai
T
T
Naito
Y
Y
D001943:Breast Neoplasms; D002455:Cell Division / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007700:Kinetics; D016685:Mitomycin / Q000494:pharmacology*; D013629:Tamoxifen / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D013629:Tamoxifen; D004958:Estradiol; D016685:Mitomycin