title: [Application of cryopreserved allograft to aortic root replacement for valve detachment case due to aortitis].
abstract: A 54-year-old male who had received an aortic valve replacement with SJM 23 about 3 years before suffered from an acute cardiac failure because of the valve detachment. From his clinical course, it was diagnosed that the aortic valve insufficiency was complicated by an aortitis. We operated on him by an aortic root replacement using a cryopreserved allograft which we prepared. A donor of the allograft was 35-year-old male, died of a subarachnoidal hemorrhage. We harvested his aortic root at an autopsy and dipped it into a nutrition medium with 10% dimethylsulfoxide. Within 10 hours from his death, we froze the tissue using a program freezer and stored it in a liquid nitrogen for 7 months. After thawing it in 37 degrees C water quickly, we rinsed the graft and used for the operation. The cell viability of the graft was confirmed by a tissue culture. Indication of the allograft valve to an aortitis case is still controversial. We think the allograft is recommendable to valve detachment case due to aortitis, because the softness of the graft decreases a compliance mismatch between the graft and the patient's annulus, which may prevent redetachment. Although we felt concern about redetachment of the conduit due to the weakness of the patient's aortic annulus, no complication regarding the allograft happened at all as yet.
authors.affiliation: Department of Cardiovascular Surgery, National Cardiovascular Center, Osaka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Motomura
authors.forename: K
authors.initials: K
authors.lastname: Kawazoe
authors.forename: H
authors.initials: H
authors.lastname: Kito
authors.forename: K
authors.initials: K
authors.lastname: Eishi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawashima
authors.forename: T
authors.initials: T
authors.lastname: Oka
mesh_terms: D001021:Aortic Valve / Q000166:cytology / Q000601:surgery*; D001022:Aortic Valve Insufficiency / Q000601:surgery; D001025:Aortitis / Q000601:surgery*; D002470:Cell Survival; D015925:Cryopreservation; D006350:Heart Valve Prosthesis; D006801:Humans; D008297:Male; D008875:Middle Aged; D011475:Prosthesis Failure; D012086:Reoperation