Characterization of the pyruvate kinase-encoding gene (pki1) of Trichoderma reesei.
The pyruvate kinase-encoding gene (pki1) from Trichoderma reesei was isolated by hybridization to the corresponding Aspergillus nidulans pkiA gene. The 1614-bp nucleotide (nt) sequence of the cloned gene codes for a 538-amino-acid protein. The coding sequence contains a single intron of 246 nt at a position identical to that of intron E in the A. nidulans gene. The PKI protein shows extensive homology to the PKIs of A. nidulans and A. niger (67%) and Saccharomyces cerevisiae (59%). The 5' non-coding sequence contains a number of motifs typical for yeast glycolytic genes, but so far only rarely found in filamentous fungi.
Schindler
M
M
Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Vienna, Austria.
Mach
R L
RL
Vollenhofer
S K
SK
Hodits
R
R
Gruber
F
F
Visser
J
J
De Graaff
L
L
Kubicek
C P
CP
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005800:Genes, Fungal; D006019:Glycolysis / Q000235:genetics; D007438:Introns; D008969:Molecular Sequence Data; D011770:Pyruvate Kinase / Q000235:genetics*; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
D004271:DNA, Fungal; D011770:Pyruvate Kinase