Microtiter plate assay for measuring the anticomplementary activity of immunoglobulins.
An anticomplementary activity (ACA) assay on a microtiter plate combined with a computer-controlled plate reader and computerized calculation of the assay result is described. The assay is a simplified version of existing ones and can be used to assay immunoglobulins at low protein concentrations. The method detects the consumption of 0.13 CH50U, corresponding to anticomplementary activity of 7.5 CH50U/g protein. The assay has a coefficient of variation of 20.4%.
Törmä
E
E
Finnish Red Cross Blood Transfusion Service, Helsinki.
Suomela
H
H
Hämäläinen
E
E
D000906:Antibodies / Q000276:immunology; D001322:Autoanalysis; D003165:Complement System Proteins / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D007118:Immunoassay / Q000379:methods; D016756:Immunoglobulins, Intravenous / Q000276:immunology; D066298:In Vitro Techniques
D000906:Antibodies; D016756:Immunoglobulins, Intravenous; D003165:Complement System Proteins