The rne gene and ribonuclease E.
The cloned rne+ gene complements temperature sensitive RNase E mutations and directs the synthesis of a polypeptide. In vitro the RNA transcribed from the rne gene directs the synthesis of a number of polypeptides, one of which is identical in size to the in vivo product of the rne gene. A rabbit reticulocyte cell free extract programmed with this RNA produced RNase E activity. Thus, it is evident that the rne gene is the structural gene for RNase E. However, the in vivo product of the cloned RNase E gene is more thermolabile than the chromosomal gene product. When cells containing the rne plasmid were treated with chloramphenicol, the pre-existing RNase E became less heat labile with time. This leads to the suggestion that in the cell RNase E undergoes post-translational modification(s).
Miczak
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A
Department of Molecular Microbiology, Washington University Medical School, St Louis, MO 63110.
Apirion
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D
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004722:Endoribonucleases / Q000235:genetics*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D006358:Hot Temperature; D010957:Plasmids; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004722:Endoribonucleases; C022537:ribonuclease E