Preferential damage to IgM production by ultraviolet B in the cells of patients with Bloom's syndrome.
In most patients with Bloom's syndrome (BS), selective IgM deficiency is commonly found. We examined proliferative responses by incorporation of [3H]-thymidine and the production of immunoglobulin after ultraviolet B (UVB) irradiation in the cells of two patients with BS. With regard to the proliferative responses in peripheral blood mononuclear cells (PBMC) cultured with pokeweed mitogen (PWM), the patients' PBMC were more sensitive to UVB irradiation than controls. Although the effect of UVB irradiation in lymphoblastoid cell lines (LCL) after 0 days of culture showed no difference between one patient and controls, the patient's LCL were more sensitive to UVB than the controls after 3 and 7 days of culture. These results suggest that the proliferative responses of the patient's LCL recovered later than those of controls. IgM production was the most sensitive to UVB in the patients' PBMC and LCL. IgG and IgA production in the patients' PBMC and LCL showed the same sensitivity as controls. From our results, it is suspected that the preferential damage to IgM production by UVB is connected with the selective IgM deficiency of BS.
Ozawa
T
T
Department of Pediatrics, Gifu University School of Medicine, Japan.
Kondo
N
N
Motoyoshi
F
F
Kato
Y
Y
Orii
T
T
D000328:Adult; D001816:Bloom Syndrome / Q000276:immunology*; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D004249:DNA Damage / Q000276:immunology*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000528:radiation effects*; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000528:radiation effects; D008297:Male; D011947:Receptors, Antigen, B-Cell / Q000097:blood / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell