title: Rapid primary microwave-glutaraldehyde fixation preserves the plasma membrane and intracellular structures of the protozoan Tritrichomonas foetus.
abstract: Tritrichomonas foetus, a pathogenic protozoan, was used as a model to analyse microwave-stimulated fixation as a procedure of preparation of biological samples for electron microscopy of thin sections and freeze-fracture replicas. Good preservation of the protozoan structure was achieved by microwave-stimulated fixation and Epon polymerization. The membrane structure, as visualized in freeze-fracture replicas, was well preserved.
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Benchimol
authors.forename: N R
authors.initials: NR
authors.lastname: Goncalves
authors.forename: W
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D005614:Freeze Fracturing; D005976:Glutaral; D008854:Microscopy, Electron / Q000379:methods; D008872:Microwaves; D016707:Tissue Fixation / Q000379:methods*; D014021:Tissue Preservation; D016835:Tritrichomonas foetus / Q000648:ultrastructure*
chemical_list: D005976:Glutaral