[The effect of 2-aminoethylisothiouronium bromide on HLA antigens].
Lymphocytes treated with a 6% and 4% AET solution after 60 or 120 minutes at 20 degrees or 37 degrees C display polyreactivity. Polyreactivity does not develop after short-term treatment (1-10 mins.). After treatment of lymphocytes with a 3% or 2% AET solution for a period of 20 or 30 mins. at 20 degrees or 37 degrees C the specific expresivity of HLA--A, B antigens rises; they are manifested, contrary to untreated lymphocytes, also by weak HLA sera. Addition of AET to the mixture of lymphocytes--HLA serum causes in the first stage of the microlymphocytotoxic test negativity of the test due to a block of the haemolytic activity of rabbit complement.
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Ustav hematologie a krevní transfúze, Praha.
D006680:HLA Antigens / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
D006680:HLA Antigens; D000338:beta-Aminoethyl Isothiourea
K otázce úcinku 2-aminoethylisothiouronium bromidu (AET) na HLA antigeny.