title: Comparative study of thermostability and structure of close homologues--barnase and binase.
abstract: Parameters of heat denaturation and intrinsic fluorescence of barnase and its close homologue, binase in the pH region 2-6 have been determined. The barnase heat denaturation (pH 2.8-5.5) proceeds according to the "all-or-none" principle. Barnase denaturation temperature is lower than that of binase and this difference increases from 2.5 degrees C at pH 5 to 7 degrees C at pH 3. Enthalpy values of barnase and binase denaturation coincide only at pH 4.5-5.5, but as far as pH decreases the barnase denaturation enthalpy decreases significantly and in this respect it differs from binase. The fluorescence and CD techniques do not reveal any distinctions in the local environment of aromatic residues in the two proteins, and the obtained difference in the parameters of intrinsic fluorescence is due to fluorescence quenching of the barnase Trp94 by the His 18 residue, absent in binase. Secondary structures of both native and denaturated proteins also do not differ. Some differences in the barnase and binase electrostatic characteristics, revealed in the character of the dipole moments distribution, have been found.
authors.affiliation: Engelhardt Institute of Molecular Biology, Acad. Sci. Russia, Moscow.
authors.forename: A A
authors.initials: AA
authors.lastname: Makarov
authors.forename: I I
authors.initials: II
authors.lastname: Protasevich
authors.forename: N V
authors.initials: NV
authors.lastname: Kuznetsova
authors.forename: B B
authors.initials: BB
authors.lastname: Fedorov
authors.forename: S V
authors.initials: SV
authors.lastname: Korolev
authors.forename: N K
authors.initials: NK
authors.lastname: Struminskaya
authors.forename: N P
authors.initials: NP
authors.lastname: Bazhulina
authors.forename: I B
authors.initials: IB
authors.lastname: Leshchinskaya
authors.forename: R W
authors.initials: RW
authors.lastname: Hartley
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
mesh_terms: D001426:Bacterial Proteins; D002151:Calorimetry; D002942:Circular Dichroism; D004722:Endoribonucleases / Q000737:chemistry*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D011487:Protein Conformation; D011489:Protein Denaturation; D012260:Ribonucleases / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D014364:Tryptophan
chemical_list: D001426:Bacterial Proteins; D014364:Tryptophan; D004722:Endoribonucleases; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease; C022543:ribonuclease T(2)