title: Activation of blood neutrophils in acute episodes of farmer's lung.
abstract: Farmer's lung often presents clinically as recurring acute episodes several hours after exposure to moldy hay. During these episodes the blood neutrophil count increases. Because activated neutrophils release toxic oxygen metabolites and proteinases, we hypothesized that the pulmonary reaction in farmer's lung may be induced by the secretory products of activated neutrophils. To evaluate this concept, we quantified the respiratory burst of separated blood neutrophils from patients with farmer's lung (n = 12) during standardized exposure tests with moldy hay. The respiratory burst of these cells was evaluated by measuring zymosan-stimulated and lucigenin-amplified chemiluminescence (CL). Asymptomatic farmers (n = 12) and normal volunteers with no prior exposure to moldy hay (n = 15) were used as control subjects. As expected, following exposure in the group of patients with farmer's lung, striking changes in VC, TLCO, and PaO2 were observed, whereas there were only minor changes in these parameters in both control groups. In all three groups a considerable increase in the blood leukocyte count was observed. The CL response of the blood neutrophils from patients with farmer's lung 6 h after exposure was significantly higher than before or 1 h after exposure (p < 0.05 for both comparisons), whereas there was no significant change in the CL response in either control group during the observation period. Our results indicate that antigen inhalation induces an increase in the number of circulating neutrophils in patients and controls, but in patients with an acute episode of farmer's lung the neutrophils are primed for an enhanced respiratory burst and may thereby damage the lung.
authors.affiliation: Department of Internal Medicine I, Klinikum Grosshadern, University of Munich, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Vogelmeier
authors.forename: F
authors.initials: F
authors.lastname: Krombach
authors.forename: S
authors.initials: S
authors.lastname: Münzing
authors.forename: G
authors.initials: G
authors.lastname: König
authors.forename: G
authors.initials: G
authors.lastname: Mazur
authors.forename: T
authors.initials: T
authors.lastname: Beinert
authors.forename: G
authors.initials: G
authors.lastname: Fruhmann
mesh_terms: D000166:Acridines / Q000494:pharmacology; D000208:Acute Disease; D000328:Adult; D000403:Airway Resistance / Q000502:physiology; D005203:Farmer's Lung / Q000097:blood*; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D006801:Humans; D007958:Leukocyte Count; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000097:blood; D011653:Pulmonary Diffusing Capacity / Q000502:physiology; D016897:Respiratory Burst / Q000502:physiology*; D014797:Vital Capacity / Q000502:physiology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000166:Acridines; C033472:10,10'-dimethyl-9,9'-biacridinium; D015054:Zymosan; D010100:Oxygen