Osteoblast-like cells have a variable mixed population of purino/nucleotide receptors.
Osteoblast-like UMR 106.06 cells respond to extracellular application of nucleotides with a fast intracellular calcium pulse (latency of about 20 s, half-width of about 10 s), as measured with fluo-3 on a confocal laser scanning system. Cross-inhibition experiments at 50 microM show that, on a cell population basis, adenosine triphosphate (ATP) strongly inhibits the effect of uridine triphosphate (UTP) or 2-methylthio-ATP (2-MeSATP) applied within 2 min after the end of the ATP-induced pulse, while prior application of UTP or 2-MeSATP only weakly inhibits the ATP effect, and UTP and 2-MeSATP weakly inhibit each other. Furthermore, there are clear differences in cross-inhibition between individual cells. Our measurements provide strong evidence that these cells have at least two types of purino/nucleotide receptors, probably P2y and P2u, with a proportion that varies between individual cells.
Yu
H
H
Medical Research Council Group in Periodontal Physiology, University of Toronto, Ontario, Canada.
Ferrier
J
J
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D000814:Aniline Compounds / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism; D011983:Receptors, Purinergic / Q000378:metabolism*; D013873:Thionucleotides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014544:Uridine Triphosphate / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D014966:Xanthenes / Q000378:metabolism
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D011983:Receptors, Purinergic; D013873:Thionucleotides; D014966:Xanthenes; C059715:Fluo-3; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate; C008501:2-methylthio-ATP