title: Properdin, a positive regulator of complement activation, is expressed in human T cell lines and peripheral blood T cells.
abstract: Properdin plays an important regulatory role in the activation of the complement system. Here we report the biosynthesis of properdin in four different human T cell lines and in T cells purified from peripheral blood. Cell sorting experiments, in conjunction with Northern blotting, showed that both CD4- and CD8-bearing populations of T cells have the potential to synthesize properdin. The functional activity of properdin secreted from these T cell lines was determined in a hemolytic assay. In view of the numerous ways in which complement activation may influence cellular immune response, the present results indicate, for the first time, a possible interaction between the complement and the T cell system.
authors.affiliation: I. Medical Clinic, University of Mainz, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Schwaeble
authors.forename: W G
authors.initials: WG
authors.lastname: Dippold
authors.forename: M K
authors.initials: MK
authors.lastname: Schäfer
authors.forename: H
authors.initials: H
authors.lastname: Pohla
authors.forename: D
authors.initials: D
authors.lastname: Jonas
authors.forename: B
authors.initials: B
authors.lastname: Luttig
authors.forename: E
authors.initials: E
authors.lastname: Weihe
authors.forename: H P
authors.initials: HP
authors.lastname: Huemer
authors.forename: M P
authors.initials: MP
authors.lastname: Dierich
authors.forename: K B
authors.initials: KB
authors.lastname: Reid
mesh_terms: D000328:Adult; D001483:Base Sequence; D015152:Blotting, Northern; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002460:Cell Line; D003167:Complement Activation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011414:Properdin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D012333:RNA, Messenger; D011414:Properdin