Effects of transforming growth factor beta on cells derived from bone and callus of patients with osteogenesis imperfecta.
We studied the influence of transforming growth factor beta (TGF-beta) on cultured bone cells derived from two patients with osteogenesis imperfecta (OI) and from human controls. Additionally, cells from a hyperplastic callus that had developed spontaneously at the femur of the patient in Case 1 and cells from a normal fracture callus were included in the study. TGF-beta increased the synthesis of total protein and collagen of all cells without changing the pattern of interstitial collagens. Proliferation was stimulated by TGF-beta in the OI bone cells from Case 1, in cells from the central part of the hyperplastic callus, and in cells from the fracture callus. In Case 2, proliferation of bone cells was decreased by low concentrations of TGF-beta. Alkaline phosphatase (AP) activity was enhanced by TGF-beta in normal human bone cells, not affected in bone cells from the patient in Case 2 or in cells from the central part of the hyperplastic callus, and inhibited in bone cells and cells from the peripheral part of the hyperplastic callus of Case 1 and in cells from the fracture callus. We conclude that TGF-beta has common and specific effects on cultured human cells derived from different types of skeletal tissues. Simultaneous stimulation of collagen synthesis and AP activity by TGF-beta was restricted to normal human bone cells and might reflect their mature state of osteoblastic differentiation. Cells derived from bone of both patients with OI, from the hyperplastic callus, and from the fracture callus showed a different response pattern to TGF-beta.(ABSTRACT TRUNCATED AT 250 WORDS)
Mörike
M
M
Department of Pediatrics, University of Ulm, Germany.
Windsheimer
E
E
Brenner
R
R
Nerlich
A
A
Bushart
G
G
Teller
W
W
Vetter
U
U
D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D002146:Bony Callus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D010013:Osteogenesis Imperfecta / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D014176:Protein Biosynthesis; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016212:Transforming Growth Factor beta; D003094:Collagen; D000469:Alkaline Phosphatase