Expression of basement membrane components in skin equivalents--influence of dermal fibroblasts.
We have made a skin equivalent constructed of fibroblasts embedded in a type I collagen, with an overlying stratified keratinocyte epithelium to examine formation of the basement membrane. We assessed the influence of the existence and species of fibroblasts in the collagen gel. Cultured human keratinocytes were well attached to the dermal equivalent. Plating efficiency was not clearly different among several types of gel. On the control and mouse fibroblast gel, sheet formation was delayed and epithelial stratification on the human fibroblast gel was more remarkable than on the control gel. On the human fibroblast gel, we observed the expression of basement membrane components (bulbous phemphigoid antigen, laminin, type IV collagen and fibronectin) between the sheet of cultured keratinocytes and the human fibroblast gel earlier than those on the control gel and mouse fibroblast gel. Type VIII collagen was not observed in any of the models at 4 weeks.
Okamoto
E
E
Department of Dermatology, Hyogo College of Medicine, Japan.
Kitano
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001324:Autoantigens / Q000032:analysis / Q000378:metabolism; D001485:Basement Membrane / Q000737:chemistry / Q000378:metabolism; D002352:Carrier Proteins; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000378:metabolism; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005353:Fibronectins / Q000032:analysis / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007797:Laminin / Q000032:analysis / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D012867:Skin / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C486268:DST protein, human; C095667:Dst protein, mouse