Structural studies of the saccharide part of the cell envelope lipopolysaccharide from Haemophilus influenzae strain AH1-3 (lic3+).
The structure of the saccharide part of the lipopolysaccharide from Haemophilus influenzae strain AH1-3 (lic3+) has been investigated. The saccharide was obtained from the lipopolysaccharide by mild acid hydrolysis followed by high-performance anion-exchange chromatography, and isolated fractions were studied by methylation analysis, NMR spectroscopy, and FAB mass spectrometry. The major saccharide is a heptasaccharide with the following structure, [formula: see text] in which Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid and PEA is 2-aminoethyl phosphate. Hep is identified as L-glycero-D-manno-heptose. The absolute configuration of the phosphorylated heptose is tentative only.
Schweda
E K
EK
Karolinska Institute, Department of Clinical Bacteriology, Huddinge Hospital, Sweden.
Hegedus
O E
OE
Borrelli
S
S
Lindberg
A A
AA
Weiser
J N
JN
Maskell
D J
DJ
Moxon
E R
ER
D002240:Carbohydrate Sequence; D006193:Haemophilus influenzae / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D006539:Heptoses / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D006539:Heptoses; D008070:Lipopolysaccharides; D009844:Oligosaccharides