A porcine model using skin graft chambers for studies on cultured keratinocytes.
In wound healing research, animal models permit an extensive tissue analysis which is not normally possible in clinical studies. A morphological comparison of human and porcine skin was made in order to identify those aspects of the wound healing process where a porcine model may help our understanding of clinical problems. We describe a porcine model for evaluating the growth of cultured keratinocytes on a variety of wound beds. Polytetrafluoroethylene skin graft chambers were used to isolate wounds and prevent epidermal healing from the skin edge. The chambers remained in situ for 5-7 weeks. We detail the surgical technique, the method of porcine keratinocyte culture and highlight some practical measures that were taken to optimise the "take" of the cultured keratinocyte grafts.
Kangesu
T
T
RAFT, Department of Research in Plastic Surgery, Mount Vernon Hospital NHS Trust, Northwood, Middx., UK.
Navsaria
H A
HA
Manek
S
S
Shurey
C B
CB
Jones
C R
CR
Fryer
P R
PR
Leigh
I M
IM
Green
C J
CJ
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D003470:Culture Media; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000187:drug effects / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011138:Polytetrafluoroethylene / Q000633:toxicity; D012867:Skin / Q000737:chemistry / Q000648:ultrastructure; D016038:Skin Transplantation / Q000502:physiology*; D013552:Swine
D003470:Culture Media; D011138:Polytetrafluoroethylene; D003094:Collagen