An AT-rich satellite DNA sequence, E180, in alfalfa (Medicago sativa).
A DNA fragment of approximately 750 bp was cloned from EcoRI-digested nuclear DNA of alfalfa (Medicago sativa). Southern blot and sequence analysis showed that the cloned DNA fragment represents a tetramer of a highly tandemly repeated DNA sequence of 185-188 bp (E180). The consensus sequence deduced from the four repeating units is 189 bp in length with an AT content of 67%. The copy number of the satellite DNA was estimated to be approximately 1.8 x 10(5) per genome and constitutes about 1% of the alfalfa genome. Sequence comparison revealed no identity to any repetitive DNA sequences that have been published to date. Digestion with HpaII and MspI indicated a high degree of methylation at the internal C of the restriction site CCGG within E180.
Xia
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Department of Crop Science, University of Guelph, Ont., Canada.
Erickson
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D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics*; D000455:Medicago sativa / Q000235:genetics; D008745:Methylation; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004276:DNA, Satellite