Crystallization of a chitosanase from Streptomyces N174.
Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 A resolution. The crystals belong to the monoclinic space group P2(1) with unit cell parameters a = 56.4 A, b = 59.6 A, c = 86.1 A and beta = 96.6 degrees. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.
Marcotte
E
E
Department of Chemistry and Biochemistry, University of Texas, Austin 78712.
Hart
P J
PJ
Boucher
I
I
Brzezinski
R
R
Robertus
J D
JD
D003460:Crystallization; D006026:Glycoside Hydrolases / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*
D006026:Glycoside Hydrolases; C019868:chitosanase