title: Recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) in the diagnosis of Aspergillus related diseases.
abstract: A. fumigatus allergen I/a (rAsp f I/a) was produced in Escherichia coli, affinity purified by Ni(2+)-chelate chromatography and refolded. rAsp f I/a binds IgE of A. fumigatus-allergic individuals as shown by ELISA, dot blots and Western blots. In addition, rAsp I/a elicited positive immediate type I skin reactions in individuals allergic to A. fumigatus but not in control individuals. Hence, rAsp f I/a can be used as a standardized antigen/allergen for an improved serological and clinical diagnosis of A. fumigatus-associated diseases.
authors.affiliation: Swiss Institute of Allergy and Asthma Research (SIAF), Davos-Platz.
authors.forename: M
authors.initials: M
authors.lastname: Moser
authors.forename: R
authors.initials: R
authors.lastname: Crameri
authors.forename: G
authors.initials: G
authors.lastname: Menz
authors.forename: M
authors.initials: M
authors.lastname: Suter
mesh_terms: D000485:Allergens; D001228:Aspergillosis / Q000175:diagnosis*; D001232:Aspergillus fumigatus / Q000737:chemistry; D006801:Humans; D008297:Male; D011994:Recombinant Proteins; D012882:Skin Tests
chemical_list: D000485:Allergens; D011994:Recombinant Proteins