title: The pathophysiologic changes following bile aspiration in a porcine lung model.
abstract: Aspiration of bile is an underpublicized aspiration syndrome. Using a porcine lung model, the physiologic response and the histopathology of lung tissue were evaluated after the intratracheal instillation of sublethal doses of bile. Twenty-one domestic swine (11 to 19 kg) were the studied population. Three groups of five swine were evaluated: a control group received intratracheal physiologic saline (pH 7.45); study group 1 received strained gastric contents (pH 2.24); and study group 2 received strained bile (pH 7.19). All animals received the solutions at 0.5 ml/kg intratracheally. Lungs of six additional animals were studied (two gastric, two bile, and two physiologic saline) after aspiration by scanning electron microscopy (SEM). A seventh untreated animal was used as the SEM control. The physiologic data were analyzed using analysis of variance for repeated measures. The SEM and histopathologic results were graded by an observer blinded to the groups and were analyzed using the analysis of variance (ANOVA) and Scheffe tests. The group with bile aspiration was consistently characterized by significant deterioration of PaO2, the alveolar-arterial (A-a) gradient, shunt fraction, and static compliance (p < 0.01); and the light histopathologic and SEM findings demonstrated pathologic changes in the bile-exposed lung (p < 0.05) greater than the gastric- or saline-exposed lungs. It is concluded that bile aspiration produces a severe chemical pneumonitis leading to noncardiac pulmonary edema.
authors.affiliation: Department of Anesthesia, University of Cincinnati College of Medicine 45267-9531.
authors.forename: D T
authors.initials: DT
authors.lastname: Porembka
authors.forename: A
authors.initials: A
authors.lastname: Kier
authors.forename: S
authors.initials: S
authors.lastname: Sehlhorst
authors.forename: S
authors.initials: S
authors.lastname: Boyce
authors.forename: J P
authors.initials: JP
authors.lastname: Orlowski
authors.forename: K
authors.initials: K
authors.lastname: Davis
mesh_terms: D000818:Animals; D001646:Bile; D001980:Bronchi / Q000473:pathology; D005750:Gastric Juice; D006439:Hemodynamics; D008168:Lung / Q000473:pathology; D010100:Oxygen / Q000097:blood; D011015:Pneumonia, Aspiration / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D013552:Swine
chemical_list: D010100:Oxygen