Two-site ELISA for quantification of the terminal C5b-9 complement complex in plasma. Use of monoclonal and polyclonal antibodies against a neoantigen of the complex.
A quantitative ELISA procedure using monoclonal and polyclonal antibodies against neoantigens of the terminal C5b-9 complement complex has been developed. The ELISA was demonstrated to be both sensitive and reproducible. The normal range for C5b-9 determinations, defined as 2.5-97.5% interval of the values obtained in 76 healthy blood donors, was 3.12-10.3 AU/ml. The presence of rheumatoid factor did not affect the determination of C5b-9 as demonstrated by immunoabsorption studies.
Accardo-Palumbo
A
A
Istituto di Clinica Medica, University of Palermo, Italy.
Triolo
G
G
Casiglia
D
D
Sallì
L
L
Giardina
E
E
Triolo
G
G
D000911:Antibodies, Monoclonal; D001172:Arthritis, Rheumatoid / Q000097:blood; D015938:Complement Membrane Attack Complex / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012217:Rheumatoid Factor / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D015938:Complement Membrane Attack Complex; D012217:Rheumatoid Factor