title: Theta-like activity in hippocampal formation slices: cholinergic-GABAergic interaction.
abstract: Brain slice preparations obtained from the rat were used to study cholinergic GABAergic interaction in mechanisms responsible for production of theta-like activity in hippocampal slices maintained in vitro. Bicuculline, a GABA-A antagonist, applied at 25 microM facilitated the effect of low concentration carbachol (25 microM) in inducing theta-like oscillations. At 100 microM, bicuculline increased the amplitude of carbachol-induced theta-like slow waves. This carbachol-bicuculline induced field potential was antagonized by a muscarinic blocker, atropine sulphate, and a GABA-A agonist, muscimol. These results provide in vitro evidence for cholinergic-GABAergic interaction in the production of hippocampal theta-like slow waves.
authors.affiliation: Department of Neurobiology, University of Lódz, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Konopacki
authors.forename: H
authors.initials: H
authors.lastname: Gołebiewski
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001640:Bicuculline / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D018756:GABA Antagonists; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009118:Muscimol / Q000494:pharmacology; D010275:Parasympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013826:Theta Rhythm / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D018756:GABA Antagonists; D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D001285:Atropine; D002217:Carbachol; D001640:Bicuculline