title: Seminal leucocyte subpopulations and sperm function in fertile and infertile Chinese men.
abstract: The level of seminal leucocytes and the prevalence of leucocytospermia was determined in a group of fertile and infertile southern Chinese men in Hong Kong. Sixteen normal fertile semen donors and 49 men with male factor infertility were studied prospectively. None had antisperm antibodies and past or present evidence of genital tract infection. Seminal leucocytes and their subsets were analysed using monoclonal antibodies and an immunocytochemical alkaline phosphatase-anti-alkaline phosphatase conjugate technique. Seminal leucocytes were detectable in 94% and 86% of the fertile and infertile men respectively, with the predominant subset being granulocytes. Leucocytospermia (> 1 x 10(6) leucocytes/ml) was found in only one of the 49 (2%) infertile men without clinical evidence of genito-urinary infection. Inverse correlations were observed between (1) the percentage of spermatozoa with normal morphology and the number of T-helper/inducer cells, (2) the linearity of sperm movement and the number of T-lymphocytes. In conclusion, the level of seminal leucocytes and the prevalence of leucocytospermia is low in infertile Chinese subjects. The effect of seminal leucocytes on sperm function in these subjects needs further evaluation.
authors.affiliation: Department of Medicine, University of Hong Kong Queen Mary Hospital.
authors.forename: A W
authors.initials: AW
authors.lastname: Kung
authors.forename: P C
authors.initials: PC
authors.lastname: Ho
authors.forename: C
authors.initials: C
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal; D002681:China; D005298:Fertility; D006801:Humans; D007248:Infertility, Male / Q000208:ethnology / Q000473:pathology*; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology; D008297:Male; D012661:Semen / Q000166:cytology*; D013094:Spermatozoa
chemical_list: D000911:Antibodies, Monoclonal