Effects of cytokines, anti-cancer agents and cocarcinogen on DNA synthesis in hair bulb cells.
We analysed the effects of cytokines, anti-cancer agents and cocarcinogen on DNA synthesis in human hair germinative cells cultured in serum-free media. Epidermal growth factor and gamma interferon were found to inhibit DNA synthesis slightly, while strong inhibition was demonstrated by doxorubicin, cytosine arabinoside and tetradecanoyl-phorbolacetate. Basic fibroblast growth factor had very little influence on DNA synthesis. This organ culture model in serum-free media is a useful method by which to examine the effects of various cytokines and drugs on DNA synthesis in hair germinative cells and/or to study the pathogenesis of various alopecia diseases.
Imai
R
R
Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.
Jindo
T
T
Mochida
K
K
Shimaoka
S
S
Takamori
K
K
Ogawa
H
H
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003043:Cocarcinogenesis; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D004317:Doxorubicin / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006197:Hair / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000970:Antineoplastic Agents; D016895:Culture Media, Serum-Free; D016207:Cytokines; D003561:Cytarabine; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D004317:Doxorubicin; D007371:Interferon-gamma; D004247:DNA; D013755:Tetradecanoylphorbol Acetate