title: Effect of OP-41483.alpha-CD, a prostacyclin analog, on a clamp-induced endothelial injury in rats.
abstract: We studied the effect of OP-41483.alpha-CD, a stable prostacyclin analog, on clamp-induced endothelial injury in rats. The injury was assessed by vascular Evans blue leakage and using a scanning electron microscope. OP-41483.alpha-CD significantly reduced the Evans blue leakage at doses of 30 and 100 ng/kg/min. PGE1.CD was also found to show an equipotent inhibitory action on the dye leakage. From scanning electron microscopic observations, a moderate degree of intimal defects, microvillous projections and platelet adhesions at the luminal surface were seen in the specimens from OP-41483.alpha-CD (30 and 100 ng/kg/min) treated rats. Furthermore, OP-41483.alpha-CD, PGE1.CD and Dibutyryl cyclic AMP (DbcAMP) were found to accelerate a proliferation of cultured bovine endothelial cells in a dose-dependent manner in vitro. Taken together, these data indicate that the endothelial regenerative effect of OP-41483.alpha-CD could contribute to healing of clamp-induced endothelial injury and it may be an important therapeutic drug to protect vascular intimal injury.
authors.affiliation: Minase Research Institute, Ono Pharmaceutical Co., Ltd., Japan.
authors.forename: R
authors.initials: R
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Shiroya
authors.forename: M
authors.initials: M
authors.lastname: Naka
authors.forename: N
authors.initials: N
authors.lastname: Omawari
authors.forename: K
authors.initials: K
authors.lastname: Shinomiya
authors.forename: B
authors.initials: B
authors.lastname: Fujitani
authors.forename: H
authors.initials: H
authors.lastname: Aishita
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects / Q000648:ultrastructure; D020212:Carotid Artery Injuries; D002455:Cell Division; D003250:Constriction; D003505:Cyclodextrins / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000293:injuries / Q000648:ultrastructure; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D005070:Evans Blue; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar; D047391:alpha-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D047391:alpha-Cyclodextrins; C051518:OP 41483-alpha-cyclodextrin; D005070:Evans Blue; D011464:Epoprostenol