title: Endocytosis of alpha 1-acid glycoprotein variants by human monocytic lineage cells.
abstract: Human alpha 1-acid glycoprotein (AGP or orosomucoid) is a major glycoprotein of plasma. AGP can be separated on immobilized concanavalin A into three variants bearing none (AGP A), one (AGP B) or two (AGP C) biantennary glycans. In this paper, we show, using flow cytometry and confocal microscopy, that AGP C which is eluted from concanavalin A with mannose, binds to human monocytes, monocyte-derived macrophages as well as human promonocytic cell lines such as THP1 or U937. Conversely HL60, a promyelocytic cell line, does not express the surface AGP C binding protein. AGP C is internalized and degraded with an efficiency depending on the state of differentiation of these cells. In contrast, AGP A which is not recognized by concanavalin A, does not bind to any of these cells.
authors.affiliation: Département de Biochimie des Glycoconjugués et Lectines Endogènes, CNRS, INSERM et Université d'Orléans, France.
authors.forename: V
authors.initials: V
authors.lastname: Carpentier
authors.forename: P
authors.initials: P
authors.lastname: Midoux
authors.forename: M
authors.initials: M
authors.lastname: Monsigny
authors.forename: A C
authors.initials: AC
authors.lastname: Roche
mesh_terms: D002460:Cell Line; D016922:Cellular Senescence / Q000502:physiology; D004705:Endocytosis / Q000502:physiology*; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000276:immunology; D008853:Microscopy / Q000379:methods; D009000:Monocytes / Q000276:immunology*; D009961:Orosomucoid / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
chemical_list: D006023:Glycoproteins; D009961:Orosomucoid