title: Correlation of antibody titres induced by vaccination with protection in mouse typhoid.
abstract: The ELISA method was used to titrate the humoral immune response in vaccinated mice. When mice were given two doses of a heat-killed salmonella vaccine 6 days apart, there was a steady but low-level increase of antibody synthesis. In contrast, if a booster vaccination was administered 21 days after the primary inoculation, the anamnestic response produced a significantly greater antibody titre, rapidly reaching its peak within 10 days. Such a heightened humoral response induced in the genetically susceptible C57BL/6J mice also coincided with effective protection against an otherwise lethal challenge with Salmonella typhimurium.
authors.affiliation: Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
authors.forename: H R
authors.initials: HR
authors.lastname: Xu
authors.forename: H S
authors.initials: HS
authors.lastname: Hsu
authors.forename: C W
authors.initials: CW
authors.lastname: Moncure
authors.forename: R A
authors.initials: RA
authors.lastname: King
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000276:immunology*; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines