title: Testosterone, a follicular regulator: key to anovulation.
abstract: To study the interrelationships of steroids within the follicle, combined 6-h infusions of [3H]dehydroepiandrosterone sulfate and [14C] testosterone ([14C]T) were performed in four normal women treated with menotropins who were undergoing medically indicated surgery. The concentrations of tracer and/or nonisotopic dehydroepiandrosterone sulfate, androst-5-ene-3 beta,17 beta-diol sulfate, androst-5-ene-3 beta,17 beta-diol, dehydroepiandrosterone, androstenedione, T, dihydrotestosterone, estrone (E1), and estradiol (E2) were determined in arterial and venous blood and follicular fluid. The log-transformed product/precursor ratio of [3H]dihydrotestosterone/[3H]T in follicular fluid was negatively correlated with the log-transformed follicular concentrations of E1 (P = 0.01) and E2 (P = 0.02), suggesting a reciprocal relationship between 5 alpha-reductase and follicular E1 and E2. E2 and T were positively correlated in follicular fluid (r = 0.84; P = 0.0003), suggesting a stimulatory action of follicular T on aromatase. These findings along with extensive published data suggest that follicular T functions as a follicular regulator, enhancing follicular aromatase activity when adequate amounts of FSH are available. These conclusions have important implications with regard to mechanisms for selecting the dominant follicle and producing atresia in the remaining cohort of follicles, and they describe a final common path in the pathophysiology of anovulation.
authors.affiliation: Women and Infants' Hospital, Providence, Rhode Island.
authors.forename: R V
authors.initials: RV
authors.lastname: Haning
authors.forename: R J
authors.initials: RJ
authors.lastname: Hackett
authors.forename: C A
authors.initials: CA
authors.lastname: Flood
authors.forename: J S
authors.initials: JS
authors.lastname: Loughlin
authors.forename: Q Y
authors.initials: QY
authors.lastname: Zhao
authors.forename: C
authors.initials: C
authors.lastname: Longcope
mesh_terms: D000858:Anovulation / Q000209:etiology*; D001141:Aromatase / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives / Q000494:pharmacology; D019314:Dehydroepiandrosterone Sulfate; D013196:Dihydrotestosterone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006801:Humans; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology*; D012044:Regression Analysis; D013739:Testosterone / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D013196:Dihydrotestosterone; D004970:Estrone; D013739:Testosterone; D003687:Dehydroepiandrosterone; D004958:Estradiol; D019314:Dehydroepiandrosterone Sulfate; D001141:Aromatase