Transfer nuclear Overhauser effect study of the conformation of oxytocin bound to bovine neurophysin I.
This study reports the structure of the peptide hormone oxytocin bound to its carrier protein, neurophysin I, obtained by nuclear magnetic resonance techniques. At the pH value of 2.1 in our experiments, the ligand is in fast exchange with its carrier protein, allowing the use of transfer-NOE methods. The number of distance constraints for the peptide being limited, considerable attention has been paid to an accurate distance determination. The resulting accurate distance limits were used as input for a distance geometry calculation followed by a restrained molecular dynamics run. Convergence to a well-defined family of structures for oxytocin in its bound state was reached. Both the backbone and the side-chain conformations differ between the bound form and the crystal structure of free oxytocin [Wood, S. P., et al. (1986) Science 232, 633]. These differences, as well as other structural features of the bound form, are discussed in terms of interactions made with the carrier protein. Transfer-NOE experiments at low peptide protein ratios provide direct experimental evidence for contacts between the oxytocin Tyr2 residue and an aromatic residue of neurophysin. The resonance assignments of the aromatic groups [Whittaker, B. A., et al. (1985) Biochemistry 24, 2782] together with the recently published X-ray structure of the neurophysin II protein complexed with a dipeptide [Chen et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 4240] allow us to assign the aromatic signal on the protein to the neurophysin Phe22 residue.
Lippens
G
G
UCMB and Corvas at UCMB, Free University of Brussels, Belgium.
Hallenga
K
K
Van Belle
D
D
Wodak
S J
SJ
Nirmala
N R
NR
Hill
P
P
Russell
K C
KC
Smith
D D
DD
Hruby
V J
VJ
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009481:Neurophysins / Q000378:metabolism*; D010121:Oxytocin / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D014961:X-Ray Diffraction
D009481:Neurophysins; D010121:Oxytocin