title: Autoradiographic localization of 5-hydroxytryptamine1A, 5-hydroxytryptamine1B and 5-hydroxytryptamine1C/2 binding sites in the rat spinal cord.
abstract: Autoradiographic techniques revealed that 5-hydroxytryptamine1A, 5-hydroxytryptamine1B and 5-hydroxytryptamine1C/2 binding sites are differentially distributed in the spinal cords of adult male rats. In the dorsal horn, 5-hydroxytryptamine1A sites were dense in all laminae; 5-hydroxytryptamine1B sites were more dense in laminae I, III and IV than in lamina II; while 5-hydroxytryptamine1C/2 sites were very sparse. The dorsal commissure gray matter also exhibited very dense 5-hydroxytryptamine1A and 5-hydroxytryptamine1B binding. In the intermediate and central gray matter, all three sites were moderately dense at autonomic levels, with exceptionally dense1C/2 binding restricted to the intermediolateral nucleus at rostral thoracic levels. In the ventral horn, 5-hydroxytryptamine1A and 5-hydroxytryptamine1B sites were very sparse (except for very dense 5-hydroxytryptamine1A sites located in the dorsolateral nucleus of the pudendal nerve), while 5-hydroxytryptamine1C/2 sites were relatively dense in motor nuclei. Surprisingly, 5-hydroxytryptamine1B sites were moderately dense in the dorsal column corticospinal tract. These studies will provide an anatomical perspective for interpretation of the complex role of 5-hydroxytryptamine in regulating spinal cord function.
authors.affiliation: Lilly Research Laboratories, A division of Eli Lilly and Co., Indianapolis, IN 46285.
authors.forename: K B
authors.initials: KB
authors.lastname: Thor
authors.forename: S
authors.initials: S
authors.lastname: Nickolaus
authors.forename: C J
authors.initials: CJ
authors.lastname: Helke
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000378:metabolism; D000662:Amphetamines / Q000378:metabolism; D000818:Animals; D020120:Iodocyanopindolol; D008297:Male; D009046:Motor Neurons / Q000737:chemistry; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000033:anatomy & histology; D011985:Receptors, Serotonin / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D012701:Serotonin / Q000502:physiology*; D013116:Spinal Cord / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D000662:Amphetamines; D011985:Receptors, Serotonin; D012701:Serotonin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D020120:Iodocyanopindolol; D010869:Pindolol; C015952:4-iodo-2,5-dimethoxyphenylisopropylamine