Evaluation of new therapy for eradication of H. pylori infection in nude mouse model.
OBJECTIVE
developing a new therapy for eradication of H. pylori by using the nude mouse model.


METHODS
By quantifying the number of colonies of Helicobacter pylori and the score of H. pylori-associated gastritis from the gastric tissue following different drug regimens in inoculated nude mice, we evaluated the effectiveness of each regimen, including a new drug, plaunotol. Drugs were administered daily for a 1-wk period, beginning 4 wk after inoculation.


RESULTS
In the examination after therapy, the number of colonies of H. pylori and the score of gastritis in the triple-therapy group were significantly lower than in any of the singly- and dual-drug groups or control group from 5 wk to the end of the study after inoculation. Inflammation of the stomach was less apparent in the treatment groups than in the control group.


CONCLUSIONS
With the nude mouse model, we quantitatively demonstrated that the new triple therapy is the most effective therapy for eradication of H. pylori.
Karita
M
M
First Department of Internal Medicine, Yamaguchi University, School of Medicine, Japan.
Li
Q
Q
Okita
K
K
D000658:Amoxicillin / Q000627:therapeutic use; D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use; D004224:Diterpenes; D004353:Drug Evaluation, Preclinical; D004359:Drug Therapy, Combination; D005233:Fatty Alcohols / Q000627:therapeutic use; D005753:Gastric Mucosa / Q000473:pathology; D005756:Gastritis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000254:growth & development; D008297:Male; D008795:Metronidazole / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude
D000897:Anti-Ulcer Agents; D004224:Diterpenes; D005233:Fatty Alcohols; D008795:Metronidazole; D000658:Amoxicillin; C047686:plaunotol