title: Mucin-like glycoproteins linked to the membrane by glycosylphosphatidylinositol anchor are the major acceptors of sialic acid in a reaction catalyzed by trans-sialidase in metacyclic forms of Trypanosoma cruzi.
abstract: We have previously shown that 35- and 50-kDa glycoconjugates of cultured metacyclic trypomastigotes participate in the attachment of parasites to mammalian cells. Here we show that when metacyclic trypomastigotes are incubated with [3H]sialyllactose, most of the sialic acid is transferred to these 35/50-kDa molecules in a reaction catalyzed by a parasite transsialidase. The sialic acid is incorporated in oligosaccharides of about 10 glucose units in size that are released from the glycoconjugate by mild alkaline hydrolysis. Compositional analysis reveals that the 35/50-kDa molecules are highly glycosylated proteins rich in threonine, galactose, N-acetyl-glucosamine and sialic acid. These glycoproteins can be labeled in vivo with [3H]palmitate, and the labeled fatty acid is released by glycosylphosphatidylinositol specific phospholipases C. This result, associated with the fact that they contain mannose, ethanolamine, myo-inositol, and lipid, indicate that these glycoproteins are anchored to the membrane by glycosylphosphatidylinositol. During cell invasion, these molecules appear to be capped and locally released by the parasite.
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, São Paulo, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Schenkman
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson
authors.forename: N
authors.initials: N
authors.lastname: Heise
authors.forename: M L
authors.initials: ML
authors.lastname: de Almeida
authors.forename: R A
authors.initials: RA
authors.lastname: Mortara
authors.forename: N
authors.initials: N
authors.lastname: Yoshida
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000302:isolation & purification; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009077:Mucins / Q000378:metabolism; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000378:metabolism*; D015800:Protozoan Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism*; D014316:Tritium; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000502:physiology
chemical_list: D000596:Amino Acids; D000953:Antigens, Protozoan; D006023:Glycoproteins; D017261:Glycosylphosphatidylinositols; D009077:Mucins; D015800:Protozoan Proteins; D012794:Sialic Acids; D014316:Tritium; C069715:trans-sialidase; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid