An inwardly rectifying K+ channel in human adenomatous parathyroid cells.
The ion channel populations of cells prepared from human adenomatous parathyroid glands have been investigated using standard patch-clamp techniques with pipettes containing isotonic K(+)-rich solutions. The principal channel type observed was an inwardly rectifying K+ channel of 35 pS conductance. In addition, we have frequently observed a lower conductance (12 pS) K+ channel that appeared to conduct current in both directions. In addition to these K+ channels, we have observed non-selective cation channels and possibly CI- channels. Although we have observed occasional current transitions that might have arisen from a large conductance K+ channel similar to that observed in rat and bovine parathyroid cells, we have not been able positively to identify such a channel in human parathyroid cells, either in cell-attached or in excised inside-out patches.
Conigrave
A D
AD
Department of Physiology, University of Sydney, New South Wales, Australia.
Poronnik
P
P
Komwatana
P
P
Delbridge
L
L
Young
J A
JA
Cook
D I
DI
D000236:Adenoma / Q000737:chemistry* / Q000150:complications; D004594:Electrophysiology; D006801:Humans; D006961:Hyperparathyroidism / Q000209:etiology / Q000378:metabolism; D015640:Ion Channel Gating; D008564:Membrane Potentials; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000502:physiology*; D010282:Parathyroid Neoplasms / Q000737:chemistry* / Q000150:complications; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000502:physiology*
D009363:Neoplasm Proteins; D015221:Potassium Channels; D011188:Potassium