title: Airway allergy to trimellitic anhydride in guinea pigs: different time courses of IgG1 titer and airway responses to allergen challenge.
abstract: BACKGROUND
  Trimellitic anhydride (TMA) is a low molecular weight chemical that may cause occupational asthma in human beings. The objectives of this study were to determine the time course of immune and airway responses to TMA in guinea pigs and to relate the immunologic response to the immediate responses in lung resistance (RL) and plasma exudation induced by allergen challenge.
  
  
  METHODS
  We studied the effects of time course after sensitization on airway response to TMA in guinea pigs actively sensitized to free TMA, given by intradermal injection (0.1 ml of 0.3% TMA in corn oil). During weeks 1, 2, 3, 5, and 8 after sensitization, anesthetized animals were challenged with TMA conjugated to guinea pig serum albumin (TMA-GPSA), instilled via the airway route. Nonsensitized animals were challenged with the same amount of conjugate 4 weeks after intradermal injection of corn oil only. In the same animal, we measured both RL to monitor airflow obstruction and extravasation of Evans blue dye (20 mg/kg) to quantify airway plasma exudation.
  
  
  RESULTS
  Instillation of TMA-GPSA (0.5%; 50 microliters) into the tracheal lumen caused a significant increase in RL, reaching a maximum at 2.5 minutes after the instillation in the 1-week group (9.0 +/- 5.9 cm H2O/ml/sec) and between 5 and 6 minutes in the 2-, 3-, 5-, and 8-week groups (9.4 +/- 4.8, 12.7 +/- 5.5, 3.7 +/- 1.1, and 1.7 +/- 0.2 cm H2O/ml/sec, respectively). The maximal increase in RL after the challenge in nonsensitized animals was 0.39 +/- 0.05 cm H2O/ml/sec. TMA-GPSA also produced significant extravasation of Evans blue dye at all airway levels in the sensitized groups, and the amount of dye in the peripheral airways was significantly greater than that in the trachea. Furthermore, the level of Evans blue dye in airway tissue increased with the time after sensitization, up to the latest time point tested (8 weeks). Specific IgG1 antibodies to TMA-GPSA demonstrated by ELISA were detected in all animals in the 3-, 5-, and 8-week groups, with maximal levels 5 weeks after sensitization. Specific IgG1 titers to TMA-GPSA significantly correlated with the level of Evans blue dye induced by challenge with TMA-GPSA but not with the increase in RL.
  
  
  CONCLUSIONS
  Intradermal sensitization to free TMA induces specific airway allergy for a long period after sensitization. Specific IgG1 antibodies to allergen may influence allergen-induced plasma exudation rather than the airflow obstruction in this animal model of TMA-induced asthma.
authors.affiliation: Division of Clinical Pharmacology (Department of Pharmacology), Göteborg University, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Arakawa
authors.forename: J
authors.initials: J
authors.lastname: Lötvall
authors.forename: I
authors.initials: I
authors.lastname: Kawikova
authors.forename: R
authors.initials: R
authors.lastname: Tee
authors.forename: J
authors.initials: J
authors.lastname: Hayes
authors.forename: C G
authors.initials: CG
authors.lastname: Löfdahl
authors.forename: A J
authors.initials: AJ
authors.lastname: Taylor
authors.forename: B E
authors.initials: BE
authors.lastname: Skoogh
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000276:immunology; D001985:Bronchial Provocation Tests; D002199:Capillary Permeability / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005070:Evans Blue; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D010796:Phthalic Anhydrides / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000276:immunology*; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D010796:Phthalic Anhydrides; D005070:Evans Blue; C015559:trimellitic anhydride