Isolation and characterization of cock brain coated vesicles in comparison with porcine brain coated vesicles.
1. Cock brain coated vesicles (CBCVs) were isolated and compared with porcine brain coated vesicles (PBCVs). 2. The fine structure of CBCVs was quite similar to that of PBCVs. 3. Size distribution of CBCVs showed a rather single population, whereas that of PBCVs seemed to consist at least two subpopulations. 4. CBCVs possessed proteins quite characteristic of coated vesicles (CVs). 5. The protein composition of CBCVs was very like that of PBCVs with the exception of clathrin light chain b. 6. A small difference in the electrophoretic mobility existed between CBCVs and PBCVs. 7. The density of CBCVs was slightly greater than that of PBCVs.
Yora
T
T
Department of Medical Technology, Saitama Medical School Junior College, Japan.
Sakagishi
Y
Y
Nakamura
H
H
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D002458:Cell Fractionation; D002645:Chickens / Q000033:anatomy & histology*; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D015388:Organelles / Q000737:chemistry / Q000648:ultrastructure*; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D013045:Species Specificity; D013552:Swine / Q000033:anatomy & histology*
D011506:Proteins