title: Cinnamamide analogs as inhibitors of protein tyrosine kinases.
abstract: Protein tyrosine kinases (PTK) are important signal transducing enzymes involved in the modulation of normal cellular growth and differentiation and have been associated with the etiology of various human cancers. The development of properly designed inhibitors, which block their function by interfering with the substrate binding, may therefore offer an unique target for selective anticancer chemotherapy. Here we describe synthesis and biochemical testing of a novel series of non-peptide PTK inhibitors which have as characteristic active pharmacophore the cinnamamide moiety. For testing we used an exogenous substrate kinase assay based on the phosphorylation of (Val5)-angiotensin II with radiolabelled ATP by the catalytic domain of the PTK encoded by the v-abl oncogene (p45 v-abl). The most potent compounds were found in the class of 3-arylidene-2-oxindoles (II) with IC50 values in the 1 microM range. Among these the 2-tetralylmethylene-, 4-quinolylmethylene-, 5-quinolylmethylene- and 3-indolylmethylene-2-oxindole compounds of formulae 16, 20, 21 and 24 respectively were selected for further investigation.
authors.affiliation: Ricerca e Sviluppo, Farmitalia Carlo Erba Srl, Milano, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Buzzetti
authors.forename: M G
authors.initials: MG
authors.lastname: Brasca
authors.forename: A
authors.initials: A
authors.lastname: Crugnola
authors.forename: S
authors.initials: S
authors.lastname: Fustinoni
authors.forename: A
authors.initials: A
authors.lastname: Longo
authors.forename: S
authors.initials: S
authors.lastname: Penco
authors.forename: P
authors.initials: P
authors.lastname: Dalla Zonca
authors.forename: P M
authors.initials: PM
authors.lastname: Comoglio
mesh_terms: D000097:Acetonitriles / Q000138:chemical synthesis* / Q000494:pharmacology; D000818:Animals; D002934:Cinnamates / Q000138:chemical synthesis*; D007211:Indoles / Q000138:chemical synthesis* / Q000494:pharmacology; D051379:Mice; D009570:Nitriles / Q000138:chemical synthesis* / Q000494:pharmacology; D010648:Phenylacetates / Q000138:chemical synthesis* / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D013329:Structure-Activity Relationship
chemical_list: D000097:Acetonitriles; D002934:Cinnamates; D007211:Indoles; D009570:Nitriles; D010648:Phenylacetates; D011505:Protein-Tyrosine Kinases