title: Direct double antibody sandwich immunoassay for Pseudomonas aeruginosa elastase.
abstract: A direct sandwich enzyme immunoassay was developed in order to quantify Pseudomonas aeruginosa elastase. As a solid phase the wells of a microtitre plate were coated with specific IgG and horseradish peroxidase labelled IgG was used as the second antibody. The detection limit of the assay was 0.26 ng/ml and a good agreement was found with elastolytic activity determined using elastin-Congo red. This assay was simple, specific, sensitive and reproducible, and permits the determination of low levels of elastase.
authors.affiliation: CERM, Hôpital Renée Sabran, Giens, Hyères, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Jaffar-Bandjee
authors.forename: J
authors.initials: J
authors.lastname: Carrere
authors.forename: A
authors.initials: A
authors.lastname: Lazdunski
authors.forename: O
authors.initials: O
authors.lastname: Guy-Crotte
authors.forename: C
authors.initials: C
authors.lastname: Galabert
mesh_terms: D003550:Cystic Fibrosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010196:Pancreatic Elastase / Q000032:analysis* / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000276:immunology
chemical_list: D010196:Pancreatic Elastase