cDNAs sequences encoding cytochrome P450 (CYP71 family) from eggplant seedlings.
Three cytochrome P450 (P450) cDNAs were isolated from an eggplant hypocotyl cDNA library using eggplant CYP75 cDNA as a probe. These cDNAs have greater than 65% identity in their amino acid sequences, indicating that they belong to the same family. Comparison of these with P450 proteins from other sources showed that the protein with the greatest degree of homology is CYP71, isolated from avocado fruits (approximately 48%). We concluded that they are novel members of the CYP71 gene family (CYP71A2, 3 and 4). We have examined the level of mRNA transcripts from the CYP71 family in eggplant hypocotyl tissues and petunia flower buds, and found that the level of transcripts is developmentally regulated in the flower bud.
Umemoto
N
N
Central Laboratory for Key Technology, Kirin Brewery Co., Yokohama, Japan.
Kobayashi
O
O
Ishizaki-Nishizawa
O
O
Toguri
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000201:enzymology*
D012333:RNA, Messenger; D004247:DNA; D003577:Cytochrome P-450 Enzyme System