title: [Determination of total IgE (tIgE) and specific IgE (sIgE) using the IMX and MATRIX techniques].
abstract: This study, on 185 subjects, has given evaluation of total IgE by the IMX technique in comparison with CAP PRIST, and of the MATRIX* technique for the measurement of sIgE in comparison with the reference technique of CAP RAST**, skin tests and clinical history. The two techniques of CAP and MATRIX are judged to be comparable (Correlation percentage 88.2%). The correlation of MATRIX with skin tests and clinical history is satisfactory (respectively 85.9% and 95%). The results were obtained with four allergens (D. pteronyssinus, Timothy grass, cat and dog). Separate assays confirmed the reliability of the technique.
authors.affiliation: Laboratoire d'Immuno-allergologie--CHRU, Angers.
authors.forename: A
authors.initials: A
authors.lastname: Sabbah
authors.forename: M
authors.initials: M
authors.lastname: Drouet
authors.forename: M G
authors.initials: MG
authors.lastname: Lauret
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000485:Allergens / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D008925:Mites / Q000276:immunology; D011058:Pollen / Q000276:immunology; D011852:Radioallergosorbent Test; D011864:Radioimmunosorbent Test; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012882:Skin Tests
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E
vernacular_title: Dosage des IgE totales (tIgE) et des IgE spécifiques (sIgE) par les techniques IMX et MATRIX.