[Promoting effect of panaxatriol ginsenoside on gene expression of human interleukin-1].
Effect of panaxatriol ginsenoside (PG) on interleukin-1 (IL-1) gene expression was studied by using wheat germ extract (cell-free translation system) and IL-1 bioassay. The results showed that IL-1 production increased by 40% (487-682 U.ml-1) at maximum during cell culture (0-84 h) after PG (10 micrograms.ml-1) was added to phytohemagglutinin (PHA, 50 micrograms.ml-1) stimulated lymph node cells. Meanwhile, IL-1 mRNA from PHA combined with PG stimulated lymph node cells translated 39.5% more IL-1 than that of PHA-stimulated cells at maximum (2500-3489 U/mg RNA).
Tian
Z G
ZG
Department of Immunology, Norman Bethune University of Medical Sciences, Changchun, China.
Yang
G Z
GZ
D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects*; D036145:Ginsenosides; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
D036145:Ginsenosides; D007375:Interleukin-1; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D012503:Saponins; D014315:Triterpenes; C004563:panaxatriol