Contractile protein gene expression in serum-free cultured adult rat cardiac myocytes.
The effects of two adhesion substrates (serum and laminin) and time in culture on the expression of genes encoding myosin heavy chain (MHC) isoforms and alpha-skeletal actin were analysed in myocytes isolated from adult rat heart and maintained in serum-free culture. Relative messenger ribonucleic acid (mRNA) abundances were quantitated by dot-blot analysis. Gene expression was not influenced by the substrate used. Time in culture induced a decrease in total mRNA abundance and an up-regulation of beta-MHC and alpha-skeletal actin genes. It is proposed that atrophy of adult myocytes is associated with a pattern of gene expression similar to the fetal program.
Dubus
I
I
Unité 127 INSERM, Hôpital Lariboisière, Paris, France.
Rappaport
L
L
Barrieux
A
A
Lompré
A M
AM
Schwartz
K
K
Samuel
J L
JL
D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002448:Cell Adhesion; D003285:Contractile Proteins / Q000096:biosynthesis* / Q000235:genetics; D016895:Culture Media, Serum-Free; D004247:DNA; D015342:DNA Probes; D015870:Gene Expression; D017403:In Situ Hybridization; D007797:Laminin / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000096:biosynthesis / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000502:physiology
D000199:Actins; D003285:Contractile Proteins; D016895:Culture Media, Serum-Free; D015342:DNA Probes; D007797:Laminin; D015347:RNA Probes; D012333:RNA, Messenger; D004247:DNA; D009218:Myosins