[Establishment and characterization of a human rectal cancer cell line, SRM from primary diffuse infiltrating type cancer].
Primary diffuse infiltrative cancer of the large bowel shows poor prognosis. A human rectal cancer cell line, designated as SRM, was established from the metastatic lymph node of a 35-year-old female patient. SRM cells have been cultured with RPMI-1640 medium supplemented with 10% fetal calf serum and grew as monolayers, showing a tendency to pile up. The doubling time of this cell line was 23.0 hours, and the modal number of chromosomes was 64 at passage 14. The cells produced CA19-9 and TPA in the spent medium and formed tumors in nude mice, the histology of which was similar to that of the primary tumor. CA19-9 in the cytoplasma of the transplanted tumor cells was demonstrated by the ABC method and the c-myc oncogene was amplified in the transplanted tumor in nude mice.
Shibusawa
M
M
Department of Surgery, Showa University School of Medicine, Tokyo, Japan.
Tsunoda
Y
Y
Tsunoda
A
A
Yoshizawa
H
H
Nakao
K
K
Yasuda
N
N
Choh
H
H
Numabe
K
K
Koike
T
T
D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D000328:Adult; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D012004:Rectal Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000473:pathology
D015295:Antigens, Tumor-Associated, Carbohydrate