title: Actinobacillus pleuropneumoniae culture supernatants interfere with killing of Pasteurella multocida by swine pulmonary alveolar macrophages.
abstract: The effect of Actinobacillus pleuropneumoniae culture supernatant on swine pulmonary alveolar macrophage (PAM) functions was studied. The A. pleuropneumoniae culture supernatant was toxic to PAMs when tested by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and lactate dehydrogenase (LDH) release assays. Biological activity of the supernatant was ascribed to cytotoxins. Both the LDH and MTT assays were used for measurement of crude A. pleuropneumoniae cytotoxin concentration with good reproducibility. A preparation containing 6,800 toxic units/mL (determined by MTT assay) was used for subsequent experiments. The objective was to study the effect of crude cytotoxin on the ability of swine PAMs to kill Pasteurella multocida. Phagocytosis of opsonized P. multocida type A by PAMs was not efficient. Only 8% of incubated organisms were ingested by noncytotoxin-treated PAMs after 30 min phagocytosis. The bactericidal effect of noncytotoxin-treated PAMs only last for 60 min, after which, the rate of growth of surviving P. multocida exceeded the rate of bacterial killing by PAMs. Complete elimination of P. multocida by PAMs was not observed in this study. A total loss of ability to kill P. multocida by PAMs was seen when the PAMs were pretreated with a high concentration (340 toxic units/mL) of A. pleuropneumoniae cytotoxin. If the PAMs were pretreated with a low concentration (3.4 toxic units/mL) of cytotoxin, a significant reduction in the killing of P. multocida was still observed. The reductions in phagocytosis, phagosome-lysosome fusion (demonstrated using yeast particles of Candida albicans), and oxidative burst (demonstrated by nitro blue tetrazolium reduction (NBT) assay) may have contributed to the impaired killing of P. multocida by PAMs.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medical Science, University of Wisconsin, School of Veterinary Medicine, Madison 53706.
authors.forename: W B
authors.initials: WB
authors.lastname: Chung
authors.forename: L
authors.initials: L
authors.lastname: Bäckström
authors.forename: J
authors.initials: J
authors.lastname: McDonald
authors.forename: M T
authors.initials: MT
authors.lastname: Collins
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000276:immunology*; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D002478:Cells, Cultured; D003603:Cytotoxins / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000276:immunology*; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis / Q000187:drug effects; D010588:Phagosomes / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D013778:Tetrazolium Salts; D013844:Thiazoles; D009580:Nitroblue Tetrazolium; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue