title: The effect of IL-4 on the phenotype of a human B-cell lymphoma line (Farage) lacking immunoglobulin expression.
abstract: Farage cells do not express surface immunoglobulins (sIg) but display a high level of CD19, CD21, CD22, CD23, CD39, CD40 B-cell antigens, and various adhesion proteins, such as CD11a (LFA-1), CD29 (VLA-4), CD44, CD54 (ICAM-1), and CD58 (LFA-3). The phenotype of Farage resembled that of EBV-LCL but differed from the phenotype of Burkitt's lymphoma lines, which were CD39- CD44-, expressed a high level of CD38, and either lacked CD21 or were weakly positive. Exposure to IL-4 augmented the concentrations of CD23 and of adhesion proteins on the surface of Farage cells but diminished the expression of CD21, CD22, and CD38. IL-4 did not induce the expression of sIg on Farage cells and failed to affect the level of HLA-DR. IL-2 and TPA did not alter the level of CD21 and adhesion proteins on Farage cells. Although IL-4 induced unique changes of the antigenic pattern in Farage, no significant effect on the phenotype of Burkitt's lymphoma lines was detected after IL-4 treatment. The present study indicates that the responsiveness of B cells to IL-4 is not determined by the expression of sIg but rather is associated with the antigenic profile characteristic for non-germinal center B lymphocytes.
authors.affiliation: Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Paul Ehrlich Center for the Study of WBC, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
authors.forename: G
authors.initials: G
authors.lastname: Shubinsky
authors.forename: H
authors.initials: H
authors.lastname: Ben-Bassat
authors.forename: M
authors.initials: M
authors.lastname: Schlesinger
mesh_terms: D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D001402:B-Lymphocytes / Q000276:immunology*; D002051:Burkitt Lymphoma / Q000276:immunology; D015815:Cell Adhesion Molecules; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000494:pharmacology*; D016393:Lymphoma, B-Cell / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D015847:Interleukin-4