title: Ibuprofen treatment of endotoxin-induced mastitis in cows.
abstract: Ibuprofen treatment was compared with saline solution treatment in an endotoxin-induced experimental model of bovine mastitis. Acute mastitis was induced in healthy lactating Holstein cows (n = 12) by intramammary inoculation of 1 mg of Escherichia coli 026:B6 lipopolysaccharide in a single quarter per cow. Cows were assigned at random to ibuprofen (25 mg/kg of body weight, IV, n = 6) or 0.9% sodium chloride solution control (1.25 ml/kg, IV, n = 6) treatment groups. Ibuprofen or saline solution was administered once, 2 hours after endotoxin administration. The clinical course of endotoxin-induced mastitis and hematologic, clinical biochemical, and plasma mineral changes were monitored and compared between ibuprofen-treated and control cows. Clinical monitoring and blood sample collection were performed at 0, 2, 4, 6, 8, 12, 24, 48, 96, and 192 hours after endotoxin challenge. Rectal temperature and heart and respiratory rates were significantly (P < or = 0.05) increased in saline treated cows, compared with cows treated with ibuprofen. Blood eosinophil count and serum phosphorus, sodium, and total carbon dioxide concentrations were significantly (P < or = 0.05) decreased in saline-treated cows, compared with cows treated with ibuprofen. Ibuprofen treatment did not significantly change ruminations per minute, electrical conductivity of milk, quarter size, or quarter inflammation. The remaining hematologic, serum biochemical, plasma mineral, and coagulation values also were not changed significantly in response to ibuprofen treatment. Untoward effects attributed to ibuprofen administration were not observed. These results indicate that ibuprofen may provide empiric relief of clinical signs of coliform-induced mastitis.
authors.affiliation: Department of Large Animal Surgery and Medicine, College of Veterinary Medicine, Auburn University, AL 36849.
authors.forename: F J
authors.initials: FJ
authors.lastname: DeGraves
authors.forename: K L
authors.initials: KL
authors.lastname: Anderson
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001777:Blood Coagulation; D001831:Body Temperature / Q000187:drug effects; D002417:Cattle; D004731:Endotoxins; D004926:Escherichia coli; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D007052:Ibuprofen / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D007262:Infusions, Intravenous; D008414:Mastitis, Bovine / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D008892:Milk / Q000737:chemistry; D012119:Respiration / Q000187:drug effects; D014131:Trace Elements / Q000097:blood
chemical_list: D004731:Endotoxins; D014131:Trace Elements; D007052:Ibuprofen