Glyoxalase II: molecular characteristics, kinetics and mechanism.
The mechanism that has been proposed for glyoxalase II [36] is summarized in Figure 3. It involves direct nucleophilic attack of an active-site histidine on the thiol ester substrate to form an acyl-imidazole intermediate which then rapidly hydrolyses. This is consistent with the known susceptibility of thiol esters to aminolysis and with the lability of acyl-imidazoles [47].
Vander Jagt
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Department of Biochemistry, University of New Mexico School of Medicine, Albuquerque 87131.
D000818:Animals; D001665:Binding Sites / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011765:Pyruvaldehyde / Q000378:metabolism; D013379:Substrate Specificity; D013869:Thiolester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D000099301:Hydroxyacylglutathione Hydrolase
D011765:Pyruvaldehyde; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase