title: 3,400 new expressed sequence tags identify diversity of transcripts in human brain.
abstract: We present the results of the partial sequencing of over 3,400 expressed sequence tags (ESTs) from human brain cDNA clones, which increases the number of distinct genes expressed in the brain, that are represented by ESTs, to about 6,000. By choosing clones in an unbiased manner, it is possible to construct a profile of the transcriptional activity of the brain at different stages. Proteins that comprise the cytoskeleton are the most abundant; however, a large variety of regulatory proteins are also seen. About half of the ESTs predicted to contain a protein-coding region have no matches in the public peptide databases and may represent new gene families.
authors.affiliation: Receptor Biochemistry and Molecular Biology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: M D
authors.initials: MD
authors.lastname: Adams
authors.forename: A R
authors.initials: AR
authors.lastname: Kerlavage
authors.forename: C
authors.initials: C
authors.lastname: Fields
authors.forename: J C
authors.initials: JC
authors.lastname: Venter
mesh_terms: D000595:Amino Acid Sequence; D001921:Brain / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D016208:Databases, Factual; D015870:Gene Expression; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D009419:Nerve Tissue Proteins; D004247:DNA