IA-1, a new marker for neuroendocrine differentiation in human lung cancer cell lines.
IA-1 is a recently isolated novel complementary DNA which encodes a protein of 510 amino acids that contains both a zinc finger DNA-binding domain and a putative prohormone domain. mRNA expression of IA-1 has been found thus far only in tumors of neuroendocrine origin. In this report we describe the expression of IA-1 mRNA in a panel of 64 human lung cancer cell lines. IA-1 mRNA was detected by Northern blot analysis in 97% (30 of 31) of small cell lung cancer cell lines. In contrast, IA-1 mRNA was detected in only 13% (4 of 30) of non-small cell lung cancer cell lines. Nine of the 30 (30%) expressed either chromogranin A mRNA or produced L-dopa decarboxylase. Four of these 9 (44%) had detectable levels of IA-1 mRNA. In most of the lung cancer cell lines examined, IA-1 showed high concordance with the other neuroendocrine markers, L-dopa decarboxylase, and chromogranin A. The one exception was a variant small cell lung cancer cell line which expressed low or nondetectable levels of L-dopa decarboxylase. IA-1 is a candidate marker of neuroendocrine differentiation of human lung tumors.
Lan
M S
MS
Laboratory of Oral Medicine, National Institute of Dental Research, NIH, Bethesda 20892.
Russell
E K
EK
Lu
J
J
Johnson
B E
BE
Notkins
A L
AL
D014408:Biomarkers, Tumor / Q000032:analysis*; D002454:Cell Differentiation; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004296:Dopa Decarboxylase / Q000032:analysis; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D053379:Chromogranin A; D002864:Chromogranins; D012333:RNA, Messenger; D004247:DNA; D004296:Dopa Decarboxylase