Circadian rhythm in aspirin (ASA)-induced injury to the stomach of the fasted rat.
Circadian rhythms exist in several gastric parameters affecting ulcerogenesis. This study investigated possible circadian rhythmicity in observed aspirin (ASA)-induced gastric lesions in the rat. In five experiments 183 rats were studied at 14 time points over a 24-hr period. Rats were adapted for three weeks on a 12-hr light schedule, fasted 18 hr, then given oral acidified ASA. One hour later, the rats were sacrificed, the stomachs were removed, stretched flat, photographed, scanned, and measured for percent area of gross lesions in the gastric corpus by computer planimetry. Cosinor rhythmometric analysis showed a significant (P < 0.001) rhythm. Mean of rhythm (MESOR) was 5.60 +/- 0.25% of total corpus. Amplitude was 25.89 +/- 6.07% of MESOR. Peak time (acrophase) was 1909 +/- 0101 hr after lights on. Thus, gastric vulnerability to ASA injury exhibited circadian rhythmicity with peak injury during the dark period in this species.
Larsen
K R
KR
Department of Medicine, Salt Lake Veterans Affairs Medical Center, Salt Lake City 84148.
Moore
J G
JG
Dayton
M T
MT
Yu
Z
Z
D000818:Animals; D001241:Aspirin / Q000009:adverse effects*; D002940:Circadian Rhythm / Q000502:physiology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000503:physiopathology*
D001241:Aspirin