The Ca(2+)-transporting ATPases of rabbit and trout exhibit different pH- and temperature-dependences.
The phosphorylation of the trout sarcoplasmic-reticulum Ca(2+)-ATPase by Pi differs in its temperature- and pH-dependence from the rabbit ATPase. In the trout enzyme, the apparent affinity for Pi and maximum phosphoenzyme values do not vary over a pH and temperature ranges that have a pronounced effect on the rabbit enzyme. The lack of temperature-dependence for phosphorylation is observed at pH 6.8. At pH 8.0, the temperature profile for phosphorylation of the trout enzyme resembles that of the rabbit at pH 6.8. The rabbit ATPase is no longer phosphorylated by Pi after solubilization with the detergent C12E9. In contrast, the trout enzyme can be phosphorylated by Pi after solubilization with C12E9, and the same levels of phosphoenzyme were obtained with the soluble and membrane-bound ATPase at both 0 degrees and 25 degrees C. In the range of 0-20 degrees C, the rates of ATP synthesis and of Ca2+ uptake by the trout ATPase are less temperature-dependent than for the rabbit enzyme. However, both isoenzymes catalyse ATP hydrolysis with similar temperature-dependences. The results raise the possibility that protonation of specific amino acid residues may contribute to the lack of temperature-dependence for phosphorylation of the trout Ca(2+)-ATPase.
Chini
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EN
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brazil.
de Toledo
F G
FG
Albuquerque
M C
MC
de Meis
L
L
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003902:Detergents; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009132:Muscles / Q000201:enzymology; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D011817:Rabbits; D012995:Solubility; D013045:Species Specificity; D013696:Temperature; D014337:Trout
D003902:Detergents; D010710:Phosphates; D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases; D002118:Calcium