Augmented passive transfer of contact sensitivity in severe combined immunodeficiency mice and its dependence of V beta 8+ cells in the picryl system.
The passive transfer of contact sensitivity using picryl chloride immune cells from H-2 syngenic BALB/c donors was analyzed in severe combined immunodeficiency (SCID) mice which lack functional T and B lymphocytes. H-2-restricted and antigen-specific contact sensitivity was transferred to SCID mice, and comparison between the level of contact sensitivity and the number of transferred cells showed a significantly more efficient transfer to SCID than to BALB/c mice. The cells passively transferring contact sensitivity were shown to carry the V beta 8 phenotype. Moreover, chromium-labeled cells from BALB/c PC1-primed donors localize normally in peripheral lymphoid organs and an increased percentage of cell arrival in the ears is clearly observed in SCID after challenge with picryl chloride.
Bellavia
A
A
Institute of General Pathology, University of Palermo, Italy.
Mattei
M
M
Dieli
F
F
Salerno
A
A
Caroleo
M C
MC
Asherson
G L
GL
Colizzi
V
V
D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology; D004423:Ear / Q000033:anatomy & histology; D005260:Female; D007116:Immunization, Passive; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D016513:Mice, SCID / Q000276:immunology*; D010641:Phenotype; D010853:Picryl Chloride / Q000009:adverse effects*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology; D014018:Tissue Distribution
D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta; D010853:Picryl Chloride