title: Transcription of human 5S rRNA genes is influenced by an upstream DNA sequence.
abstract: Six human 5S rRNA genes and gene variants and one pseudogene have been sequenced. The six genes/variants were transcribed in a HeLa cell extract with about equal efficiency. Three genes contain the Sp1 binding sequence GGGCGG in position -43 to -38 and three genes contain the Sp1 like sequence GGGCCG in this position. The six genes contain furthermore one Sp1 binding site in a position about -245 and one ATF recognition site in a position about -202. A 12 bp sequence (GGCTCTTGGGGC) found in position -32 to -21 strongly influenced the transcriptional efficiency in vitro. This 12-mer, designated the D box, has also been found upstream a 5S rRNA gene from hamster and mouse. Removal of the Sp1 binding sites had no effect on the transcription in vitro whereas the transcriptional efficiency decreased to 10% if the D box was removed from the human 5S rRNA gene.
authors.affiliation: Department of Medical Biochemistry and Genetics, Panum Institute, University of Copenhagen, Denmark.
authors.forename: J N
authors.initials: JN
authors.lastname: Nielsen
authors.forename: C
authors.initials: C
authors.lastname: Hallenberg
authors.forename: S
authors.initials: S
authors.lastname: Frederiksen
authors.forename: P D
authors.initials: PD
authors.lastname: Sørensen
authors.forename: B
authors.initials: B
authors.lastname: Lomholt
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA; D004268:DNA-Binding Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D011544:Pseudogenes; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D012341:RNA, Ribosomal, 5S; D004247:DNA