title: Heterogeneous proliferative effect of tumor necrosis factor-alpha and lymphotoxin on mitogen-activated B cells from B-chronic lymphocytic leukemia.
abstract: The proliferative effect of tumor necrosis factor-alpha (TNF-alpha) and lymphotoxin on B cells from patients with B-chronic lymphocytic leukemia (B-CLL) was studied. Fresh purified B-CLL lymphocytes showed no proliferative response to either recombinant (r) TNF-alpha or r-lymphotoxin. However, after "in vitro" activation of B-CLL lymphocytes for 2 days with Staphylococcus aureus Cowan 1 (SAC), four of seven patients showed enhanced blastogenic response in the presence of either rTNF-alpha or r-lymphotoxin. We also found that the proliferative response of SAC-activated B-CLL lymphocytes to the two cytokines was independent of that found in the presence of interleukin-2. These results demonstrate that TNF-alpha and lymphotoxin can heterogeneously support the proliferation of in vitro activated B cells from B-CLL patients and may reflect the biological heterogeneity of B-CLL disease.
authors.affiliation: Departamento de Medicina, Clinica Puerta de Hierro, Universidad Autónoma de Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Alvarez-Mon
authors.forename: J
authors.initials: J
authors.lastname: Garcia-Suarez
authors.forename: A
authors.initials: A
authors.lastname: Prieto
authors.forename: L
authors.initials: L
authors.lastname: Manzano
authors.forename: E
authors.initials: E
authors.lastname: Reyes
authors.forename: C
authors.initials: C
authors.lastname: Lorences
authors.forename: G
authors.initials: G
authors.lastname: Peraile
authors.forename: J
authors.initials: J
authors.lastname: Jorda
authors.forename: A
authors.initials: A
authors.lastname: Durantez
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D008213:Lymphocyte Activation; D008233:Lymphotoxin-alpha / Q000494:pharmacology*; D008934:Mitogens / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D007376:Interleukin-2; D008233:Lymphotoxin-alpha; D008934:Mitogens; D014409:Tumor Necrosis Factor-alpha