Application of flow cytometry to the study of antiphagocytic properties of Klebsiella pneumoniae capsular polysaccharide.
We used flow cytometry to compare the effects of whole cells and capsular polysaccharides of Klebsiella pneumoniae on the phagocytic ability ot polymorphonuclear leukocytes. Our results showed a light decrease in phagocytic activity in the presence of capsular polysaccharides, but a marked decrease with whole cells. Our findings suggest that the resistance to phagocytosis in these microorganisms is not due exclusively to their capsule, as claimed by other authors.
Ruiz
C
C
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Spain.
Sabio
J M
JM
Santos
J L
JL
Montes
M J
MJ
Olivares
E G
EG
D005434:Flow Cytometry; D006801:Humans; D007711:Klebsiella pneumoniae / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000502:physiology*
D011135:Polysaccharides, Bacterial