title: Transaminase reaction rates, transport activities and TCA cycle analysis by post-steady state 13C NMR.
abstract: In this work, we present the post-steady state analysis of the TCA cycle and a closed form solution to the rate of label washout from the C4 carbon of glutamic acid through the transaminases and the malate-aspartate shuttle and then through alpha-ketoglutarate dehydrogenase. We demonstrate using a model of this problem that the rate of label washout depends not only on the flux through alpha-ketoglutarate dehydrogenase, but most importantly on the activity of the malate-aspartate shuttle as determined by the forward and reverse fluxes through the transaminases and by the rate of transport of glutamate and alpha-ketoglutarate across the mitochondrial membrane.
authors.affiliation: Department of Medical Biochemistry, Ohio State University, Columbus 43210.
authors.forename: P M
authors.initials: PM
authors.lastname: Robitaille
authors.forename: D P
authors.initials: DP
authors.lastname: Rath
authors.forename: T E
authors.initials: TE
authors.lastname: Skinner
authors.forename: A M
authors.initials: AM
authors.lastname: Abduljalil
authors.forename: R L
authors.initials: RL
authors.lastname: Hamlin
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002952:Citric Acid Cycle / Q000502:physiology*; D004285:Dogs; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008293:Malates / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D000637:Transaminases / Q000378:metabolism*
chemical_list: D008293:Malates; D001224:Aspartic Acid; D007655:Ketoglutarate Dehydrogenase Complex; D000637:Transaminases