Kinetic study of the thermal denaturation of G actin using differential scanning calorimetry and intrinsic fluorescence spectroscopy.
Previous studies of the thermal denaturation of G actin have yielded conflicting results which have led to contradictory interpretations of the denaturation process. In their interpretations, these authors postulated that the thermal denaturation of G actin can be described by thermodynamical equilibrium laws despite the fact that this transition is irreversible. Using differential scanning calorimetry and fluorescence spectroscopy, we now show that thermal denaturation of G actin does not obey to equilibrium thermodynamics, but rather, is a two-step irreversible phenomenon under kinetic control. It can therefore be analyzed using the recently developed Sanchez-Ruiz equations.
Le Bihan
T
T
Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Trois-Rivières, Canada.
Gicquaud
C
C
D000199:Actins / Q000737:chemistry*; D000818:Animals; D002152:Calorimetry, Differential Scanning; D006358:Hot Temperature; D007700:Kinetics; D008956:Models, Chemical; D011489:Protein Denaturation; D011817:Rabbits; D013050:Spectrometry, Fluorescence
D000199:Actins