Inhibitory effect of arachidonic acid on GTPase activating protein is antagonized by 1-stearoyl, 2-arachidonoyl glycerol.
The intrinsic GTPase activity of cellular protein p21ras is strongly increased by cytosolic GTPase activating protein (GAP). The activity of this enzyme has been shown to be inhibited by arachidonic acid. We report here that this inhibition is antagonized by the presence of 1-stearoyl, 2-arachidonoyl glycerol. This effect is structure specific and dose dependent, being maximum at 200 micrograms/ml of diacylglycerol (DG). These results suggest that production of DG in response to hormones or growth factors stimulation could indirectly modulate the interaction between p21ras and GAP.
Homayoun
P
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Department of Molecular Biology, Cleveland Clinic Foundation, OH 44106.
Stacey
D W
DW
D016718:Arachidonic Acid / Q000494:pharmacology*; D004075:Diglycerides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D020690:GTPase-Activating Proteins; D011506:Proteins / Q000037:antagonists & inhibitors / Q000187:drug effects*; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D020703:ras GTPase-Activating Proteins
D004075:Diglycerides; D020690:GTPase-Activating Proteins; D011506:Proteins; D020703:ras GTPase-Activating Proteins; D016718:Arachidonic Acid; C075809:1-stearoyl-2-arachidonoylglycerol; D016283:Proto-Oncogene Proteins p21(ras)