title: Purification and partial characterization of a mitogenic lectin from the latex of Euphorbia marginata.
abstract: A lectin was purified from the latex of Euphorbia marginata by affinity chromatography on acid-treated Sepharose 6B and elution with lactose. The lectin is a glycoprotein composed of two identical subunits with M(r) 30,000, approx. The haemagglutinating activity of the lectin is not specific for any human blood group, and is inhibited by galactose and galactose-containing sugars and by gentiobiose. The lectin is strongly mitogenic for human T-lymphocytes and induces the release of interleukin-1 beta and tumor necrosis factor-alpha from cultured mononuclear cells.
authors.affiliation: Dipartimento di Patologia sperimentale, Università di Bologna, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Stirpe
authors.forename: F
authors.initials: F
authors.lastname: Licastro
authors.forename: M C
authors.initials: MC
authors.lastname: Morini
authors.forename: A
authors.initials: A
authors.lastname: Parente
authors.forename: G
authors.initials: G
authors.lastname: Savino
authors.forename: A
authors.initials: A
authors.lastname: Abbondanza
authors.forename: A
authors.initials: A
authors.lastname: Bolognesi
authors.forename: A I
authors.initials: AI
authors.lastname: Falasca
authors.forename: C A
authors.initials: CA
authors.lastname: Rossi
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D002478:Cells, Cultured; D002850:Chromatography, Gel; D016207:Cytokines / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007840:Latex / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008934:Mitogens / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry
chemical_list: D016207:Cytokines; D007840:Latex; D037102:Lectins; D008934:Mitogens; D037121:Plant Lectins