Elevated complement activities of sera from patients with high density lipoprotein deficiency (Tangier disease): the presence of normal level of clusterin and the possible implication in the atherosclerosis.
Clusterin (apolipoprotein J, SP-40,40), as well as apolipoprotein A-I (apo A-I) and apolipoprotein A-II (apo A-II), are apolipoprotein components of high density lipoprotein (HDL), but not of low density lipoprotein. In spite of the deficiencies of apo A-I, apo A-II and HDL in the sera of patients with Tangier disease, clusterin was found in them at normal level. While clusterin was present as the component of HDL with apo A-I in sera of normal donors, it was present as a protein which did not form a complex in sera of Tangier patients. SC5b-9 made from the sera of Tangier patients contained normal amounts of clusterin and was deficient in apo A-I, indicating that clusterin could be incorporated into the SC5b-9 complex without apo A-I. The complement activities of the sera of the patients were higher than those of normal donors. These results may be explained by the deficiencies of apo A-I, apo A-II and HDL in the patients, because they were suggested to be the inhibitors of the reactive haemolysis of complement. The elevated complement activities of the patients might be related to the severe atherosclerotic lesions in Tangier disease.
Choi-Miura
N H
NH
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Sakamoto
T
T
Ohtaki
S
S
Nakamura
H
H
Ishizawa
S
S
Takagi
Y
Y
Gomi
K
K
Tomita
M
M
D000328:Adult; D001161:Arteriosclerosis / Q000209:etiology*; D051152:Clusterin; D015938:Complement Membrane Attack Complex / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008055:Lipids / Q000097:blood; D008075:Lipoproteins, HDL / Q000172:deficiency*; D018832:Molecular Chaperones; D013631:Tangier Disease / Q000097:blood / Q000150:complications / Q000276:immunology*
C495242:CLU protein, human; D051152:Clusterin; D015938:Complement Membrane Attack Complex; D006023:Glycoproteins; D008055:Lipids; D008075:Lipoproteins, HDL; D018832:Molecular Chaperones; D003165:Complement System Proteins