Structural diversity of mammalian hepatic dermatan sulfates.
Dermatan sulfate-proteoglycans (DS-PGs) were extracted from rabbit, rat and bovine defatted livers by magnesium chloride extraction and DEAE-cellulose chromatography, and then submitted successively to Asahipak GS-520 gel filtration chromatography, Asahipak ES-502N anion exchange chromatography, and cellulose acetate membrane electrophoresis. The disaccharide composition of the glycosaminoglycan chains was determined by differential digestion by chondroitinase ABC, AC, ACII and/or B followed by HPLC for analysis of the resulting unsaturated disaccharides. The hepatic dermatan sulfate chains contained disulfated disaccharide units; Di-diSB and Di-diSE. The hepatic DS-PGs were divided into two groups; Di-diSE-poor DS-PGs and Di-diSE-rich DS-PGs. The iduronic acid content of Di-diSE-poor dermatan sulfate chains was higher than that of Di-diSE-rich ones.
Koshiishi
I
I
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Motoki
K
K
Qiu
G
G
Imanari
T
T
D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000737:chemistry; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003871:Dermatan Sulfate / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis; D004588:Electrophoresis, Cellulose Acetate; D008099:Liver / Q000737:chemistry* / Q000201:enzymology; D015636:Magnesium Chloride; D011817:Rabbits; D051381:Rats
D004187:Disaccharides; D015636:Magnesium Chloride; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases