Distribution in the genus Streptomyces of a homolog to nusG, a gene encoding a transcriptional antiterminator.
The presence of the vbrA gene encoding the transcriptional antiterminator NusG equivalent protein of Streptomyces virginiae was tested for in 73 Streptomyces species by Southern hybridization. Fifty-five strains (75%) including S. griseus, S. lividans TK-21 and S. coelicolor A3(2) showed clear hybridization signals, indicating wide distribution of vbrA or vbrA homologs in Streptomyces species. With hybridization patterns against 3 different probes, i.e., probes covering vbrA alone, the downstream gene rplK alone, and both vbrA-rplK, the 55 strains were classified into 4 groups. In the groups I, II and III (total 50 strains) vbrA was found to be adjacent to rplK, indicating that the gene arrangement vbrA-rplK is common in Streptomyces and that these genes may constitute a part of gene cluster encoding several components of the transcription and translation apparatus, as in Escherichia coli.
Puttikhunt
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Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
Okamoto
S
S
Nakamura
T
T
Nihira
T
T
Yamada
Y
Y
D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008040:Genetic Linkage; D010445:Peptide Elongation Factors / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D015342:DNA Probes; D029968:Escherichia coli Proteins; C070291:NusG protein, E coli; D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; D014157:Transcription Factors; C082569:VbrA protein, Streptomyces virginiae; C027624:ribosomal protein L11