Immuno-assay techniques for detecting yeasts in foods.
A brief literature review on immuno-assay of yeast cell wall antigens is given. Special attention is paid to extracellular, thermostable yeast antigens (EPS), which are released to the growth medium by many yeast species. The EPS of Saccharomyces cerevisiae and of Stephanoascus ciferrii (syn. Candida ciferrii) could be specifically and sensitively detected by a sandwich ELISA, using an IgG raised in rabbits immunized with the EPS of these yeasts. The EPS ELISA of three basidiomycetous yeasts tested was not specific, that of Geotrichum candidum was genus-specific but was not sensitive. The EPS of Zygosaccharomyces bailii could be detected in a highly specific competitive ELISA but not in a sandwich ELISA or in a latex agglutination test.
Middelhoven
W J
WJ
Laboratory of Microbiology, Wageningen Agricultural University, The Netherlands.
Notermans
S
S
D000946:Antigens, Fungal / Q000032:analysis*; D005516:Food Microbiology; D007118:Immunoassay / Q000379:methods*; D015003:Yeasts / Q000276:immunology / Q000302:isolation & purification*
D000946:Antigens, Fungal