Copper-resistant enteric bacteria from United Kingdom and Australian piggeries.
Thirty-three enteric isolates from Australian (Escherichia coli only) and United Kingdom (U.K.) (Salmonella sp., Citrobacter spp., and E. coli) piggeries were characterized with respect to their copper resistance. The copper resistance phenotypes of four new Australian E. coli isolates were comparable with that of the previously studied E. coli K-12 strain ED8739(pRJ1004), in that the resistance level in rich media was high (up to 18 mM CuSO4) and resistance was inducible. Copper resistance was transferable by conjugation from the new Australian isolates to E. coli K-12 recipients. DNA similarity between the new Australian isolates and the pco copper resistance determinant located on plasmid pRJ1004 was strong as measured by DNA-DNA hybridization; however, the copper resistance plasmids were nonidentical as indicated by the presence of restriction fragment length polymorphisms between the plasmids. DNA-DNA hybridization and polymerase chain reaction analysis demonstrated DNA homology between the pco determinant and DNA from the U.K.E. coli, Salmonella sp., and Citrobacter freundii isolates. However, the copper resistance level and inducibility were variable among the U.K. strains. Of the U.K. E. coli isolates, 1 demonstrated a high level of copper resistance, 4 exhibited intermediate resistance, and 16 showed a low level of copper resistance; all of these resistances were expressed constitutively. A single U.K. C. freundii isolate, had a high level of copper resistance, inducible by subtoxic levels of copper. Transconjugants from one E. coli and one C. freundii donor, with E. coli K-12 strain UB1637 as a recipient, showed copper resistance levels and inducibility of resistance which differed from that expressed from plasmid pRJ1004.(ABSTRACT TRUNCATED AT 250 WORDS)
Williams
J R
JR
Department of Genetics, University of Melbourne, Parkville, Australia.
Morgan
A G
AG
Rouch
D A
DA
Brown
N L
NL
Lee
B T
BT
D000822:Animal Husbandry; D000818:Animals; D001315:Australia; D001483:Base Sequence; D016971:Citrobacter freundii / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D003300:Copper / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010641:Phenotype; D015183:Restriction Mapping; D012475:Salmonella / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D013552:Swine / Q000382:microbiology*; D006113:United Kingdom
D004269:DNA, Bacterial; D003300:Copper