New aspects of neurotransplantation.
We have examined the possibility of promoting axonal regeneration within lesioned neural tissue using grafted artificial gel matrices. Polymeric matrices which feature a three-dimensional crosslinked macromolecular network were implanted into preformed lesions of the central nervous system (CNS). The host response consisted of matrix invasion by glial elements and the deposition of newly synthesized extracellular molecules. This rearrangement of the brain scarring process into an organized cellular coating promoted axonal regeneration into the gels. Entrapment of embryonic neurons and embryonal carcinoma (EC)-derived neurons, within the gels, was performed to explore the possibility of using polymer brain implants as neural graft microcarriers. Our results suggest that this approach will be useful for the delivery of cells and the promotion of axonal elongation required for successful neurotransplantation.
Woerly
S
S
Department of Biological Sciences, Keele University, Staffordshire, U.K.
Morassutti
D J
DJ
D000818:Animals; D001921:Brain / Q000473:pathology; D016380:Brain Tissue Transplantation / Q000379:methods*; D005109:Extracellular Matrix; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D009416:Nerve Regeneration; D009474:Neurons / Q000502:physiology / Q000637:transplantation*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley
D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols