title: Identification of cockroach aeroallergens from living cultures of German or American cockroaches.
abstract: The Air Sentinel and polytetrafluoroethylene (PTFE) membranes were used to capture airborne particles over living colonies of German or American cockroaches. Silver-stained SDS-PAGE gels revealed protein bands at 80, 55, 36, and several bands below the 33-kD marker. SDS-PAGE/immunoblots of PTFE eluates from German cockroach colonies incubated with serum from cockroach-sensitive individuals revealed IgE-binding bands with apparent molecular weights of 36 and 80 kD. Only the 36-kD allergen and allergens below the 33-kD marker were evident in the American PTFE eluate. ELISA analysis with a monoclonal antibody assay identified the presence of both Bla g I and Bla g II in the German PTFE eluate. No Bla g I or Bla g II could be identified in the American PTFE eluate. These studies demonstrate that in addition to Bla g I and Bla g II, several other aerosolized allergens become airborne over cockroach colonies and may be important in the environment where cockroaches are abundant.
authors.affiliation: Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock.
authors.forename: R M
authors.initials: RM
authors.lastname: Helm
authors.forename: W
authors.initials: W
authors.lastname: Burks
authors.forename: L W
authors.initials: LW
authors.lastname: Williams
authors.forename: D E
authors.initials: DE
authors.lastname: Milne
authors.forename: R J
authors.initials: RJ
authors.lastname: Brenner
mesh_terms: D000397:Air Pollution / Q000032:analysis*; D000485:Allergens / Q000032:analysis; D000818:Animals; D003058:Cockroaches / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012967:Sodium Dodecyl Sulfate
chemical_list: D000485:Allergens; D012967:Sodium Dodecyl Sulfate