Greater susceptibility to mutations in lagging strand of DNA replication in Escherichia coli than in leading strand.
Models of DNA replication in Escherichia coli involve an asymmetric DNA polymerase complex that replicates concurrently the leading and the lagging strands of double-stranded DNA. The effect of asymmetry on mutagenesis was tested with pairs of plasmids containing the unidirectional ColE1 origin of replication and a single lesion located in the leading or lagging strand. The lesion used was the covalent adduct that the chemical carcinogen N-2-acetylaminofluorene (AAF) forms with the C-8 position of guanine. Whether SOS was induced or not, mutations arose at about a 20-fold higher frequency when the AAF adduct was located in the lagging strand than when in the leading strand.
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Unité Propre de Recherche Cancérogenèse et Mutagenèse Moléculaire et Structurale, Institut de Biologie Moléculaire et Cellulaire Centre National de la Recherche Scientifique, Strasbourg, France.
Fuchs
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D015073:2-Acetylaminofluorene / Q000378:metabolism / Q000633:toxicity; D001483:Base Sequence; D004249:DNA Damage; D004256:DNA Polymerase I / Q000378:metabolism; D004258:DNA Polymerase III / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D016368:Frameshift Mutation; D006147:Guanine / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids
D004269:DNA, Bacterial; D006147:Guanine; D015073:2-Acetylaminofluorene; D004256:DNA Polymerase I; D004258:DNA Polymerase III