A novel domain sequence of connectin localized at the I band of skeletal muscle sarcomeres: homology to neurofilament subunits.
A cDNA of 4.0 kb was cloned from a chicken embryo skeletal muscle cDNA library, using an antibody to muscle elastic protein connectin (titin), the molecular mass of which is estimated to be 3,000 kDa. Immunoelectron microscopy revealed that the antiserum raised against the product of the cDNA expressed in E. coli bound to the epitopes of the connectin filament near the N2 line of chicken breast muscle sarcomeres. The predicted amino acid sequence contains eight immunoglobulin C2 motifs and regions highly homologous with the high and medium molecular weight subunits of neurofilament. In addition, there are regions homologous with desmoplakin, calsequestrin, and calpastatin.
Maruyama
K
K
Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
Endo
T
T
Kume
H
H
Kawamura
Y
Y
Kanzawa
N
N
Nakauchi
Y
Y
Kimura
S
S
Kawashima
S
S
Maruyama
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular; D064211:Connectin; D004926:Escherichia coli / Q000235:genetics; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016900:Neurofilament Proteins / Q000235:genetics; D011494:Protein Kinases; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D012518:Sarcomeres / Q000737:chemistry* / Q000648:ultrastructure*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D064211:Connectin; D009124:Muscle Proteins; D016900:Neurofilament Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D011494:Protein Kinases