Upstream regulatory sequences from two beta-conglycinin genes.
Genes encoding the beta-conglycinin seed storage proteins of soybean are expressed only in seeds during specific stages of development. The different subunits of beta-conglycinin, alpha', alpha and beta, are encoded by distinct members of a gene family. Yet there are marked differences in the regulation of the genes encoding the alpha'/alpha and beta subunits. Previous work (Chen et al., EMBO J 7: 297-302, 1988) identified a seed specific transcriptional enhancer upstream of a gene encoding the alpha' subunit. Mutations were made within this region to discern its functional components. Among those identified is a 62 bp region (between -77 and -140) that contains a vicilin box consensus sequence as well as a sequence that binds the soybean nuclear factor SEF4 in vitro. A second region, which contains a sequence homologous to the core of the legumin box consensus (i.e., CATGCAT-like or RY repeat element) at -246, was also shown to affect the activity of this enhancer in transgenic plants. A series of 5' terminal deletions were used to identify regulatory elements upstream of the beta subunit gene. Two regions were identified (from -553 to -442 and from -308 to -72) that, when deleted, led to a marked reduction in gene expression. Both of these elements contain sequences that bind SEF4 in vitro. The distal element also contains an AT-rich segment that recognizes a second nuclear factor, SEF1, in vitro. Neither of these elements contains any homology to the vicilin box consensus.
Lessard
P A
PA
Department of Biology, Washington University, St. Louis, MO 63130.
Allen
R D
RD
Fujiwara
T
T
Beachy
R N
RN
D052179:Antigens, Plant; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005916:Globulins / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D055314:Seed Storage Proteins; D012639:Seeds / Q000378:metabolism; D017384:Sequence Deletion; D030262:Soybean Proteins; D013025:Glycine max / Q000235:genetics*
D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D004247:DNA