Recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) in the diagnosis of Aspergillus related diseases.
A. fumigatus allergen I/a (rAsp f I/a) was produced in Escherichia coli, affinity purified by Ni(2+)-chelate chromatography and refolded. rAsp f I/a binds IgE of A. fumigatus-allergic individuals as shown by ELISA, dot blots and Western blots. In addition, rAsp I/a elicited positive immediate type I skin reactions in individuals allergic to A. fumigatus but not in control individuals. Hence, rAsp f I/a can be used as a standardized antigen/allergen for an improved serological and clinical diagnosis of A. fumigatus-associated diseases.
Moser
M
M
Swiss Institute of Allergy and Asthma Research (SIAF), Davos-Platz.
Crameri
R
R
Menz
G
G
Suter
M
M
D000485:Allergens; D001228:Aspergillosis / Q000175:diagnosis*; D001232:Aspergillus fumigatus / Q000737:chemistry; D006801:Humans; D008297:Male; D011994:Recombinant Proteins; D012882:Skin Tests
D000485:Allergens; D011994:Recombinant Proteins