Analysis of the expression of seven G protein alpha subunit genes in hematopoietic cells.
Various heterotrimeric GTP-binding proteins may have important functions in hematopoietic cells. There has been no comprehensive information, however, regarding their expression in various-lineage hematopoietic cells. In this report, the expression level of seven G protein alpha subunits (Gs alpha, Gi1 alpha, Gi2 alpha, Gi3 alpha, Go alpha-1, Go alpha-2, and Gx alpha) in 13 hematopoietic cell lines were analyzed by Northern blot analysis. Gi1 alpha, Go alpha-1, Go alpha-2, and Gx alpha, were expressed in a limited number of cell lines whereas Gs alpha, Gi2 alpha, and Gi3 alpha were expressed ubiquitously in nearly all cell lines tested. Gi1 alpha was expressed selectively in a pre-T cell line, P30/PHK among lymphoid-lineage cell lines and a myeloblastic cell line, KG-1 among myelomonocytoid cell lines. Go alpha-1 was expressed only in a chronic myelocytic-leukemia cell line, K-562, whereas Go alpha-2 was not expressed in any cell lines tested after ordinary exposure of autoradiography (within 4 days). Gx alpha was expressed abundantly in a rat basophilic-leukemia cell line, RBL-2H3, and expressed in K-562. A barely detectable amount of Gx alpha messenger ribonucleic acid (mRNA) was found after a long exposure of autoradiography in several cell lines with megakaryoblastoid phenotype.
Matsuoka
M
M
Fourth Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Kaziro
Y
Y
Asano
S
S
Ogata
E
E
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D002460:Cell Line; D015342:DNA Probes; D019204:GTP-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000737:chemistry*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D012333:RNA, Messenger; D019204:GTP-Binding Proteins