title: Analysis of the genomic sequence encoding the 29-kDa cysteine-rich protein of Entamoeba histolytica.
abstract: By analyzing cDNA and genomic clones coding for the 29-kDa cysteine-rich protein of Entamoeba histolytica, substantial sequence differences were found to the 5'-end of a previously described full-length cDNA coding for the same protein (Reed et al., 1992), which was reported to contain an untranslated 5'-sequence of at least 171 nucleotides, unusual for E. histolytica cDNAs. We found evidence that the cDNA published by Reed et al. (1992) represents a hybridclone composed of two unrelated sequences and that the gene coding for the 29-kDa molecule comprises all of the features typical for E. histolytica genes. A data base analysis revealed substantial sequence homology of the 29-kDa protein to a class of polypeptides found in prokaryotic organisms that may be involved in the inactivation of hydrogen peroxide.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Bruchhaus
authors.forename: E
authors.initials: E
authors.lastname: Tannich
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry; D004748:Entamoeba histolytica / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D016053:RNA, Protozoan / Q000032:analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D016053:RNA, Protozoan; C083035:cysteine-rich protein p29, Entamoeba histolytica