Newly established murine pituitary folliculo-stellate-like cell line (TtT/GF) secretes potent pituitary glandular cell survival factors, one of which corresponds to metalloproteinase inhibitor.
A mouse folliculo-stellate cell (FS cell) line, TtT/GF, was found to secrete a potent survival factor influencing a somatotropic cell line (MtT/S) in serum-free medium. A three-step chromatography purification procedure using heparin-affinity chromatography, anion-exchange chromatography and gel-filtration chromatography revealed that the factor was a 27 kDa protein. Amino-terminal sequence analysis showed that the N-terminal 20 amino acids of the 27 kDa protein corresponded to those of human tissue inhibitor of metalloproteinase-II (TIMP-II). These results indicate that FS cells secrete TIMP-II, which probably protects the composition of the extracellular matrix and acts as a cell survival factor for endocrine cells in the anterior pituitary gland.
Matsumoto
H
H
Discovery Research Laboratories I, Takeda Chemical Industries, Ltd., Tsukuba, Japan.
Ishibashi
Y
Y
Ohtaki
T
T
Hasegawa
Y
Y
Koyama
C
C
Inoue
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D019716:Tissue Inhibitor of Metalloproteinase-2
D009363:Neoplasm Proteins; D019716:Tissue Inhibitor of Metalloproteinase-2