title: Stimulatory effect of calponin on myosin ATPase activity.
abstract: Calponin, a calmodulin-binding protein of smooth muscle that inhibits the actin-myosin interaction by binding to actin, was shown to bind to myosin and to stimulate the ATPase activity of myosin to some extent. Actin abolished this myosin-linked, stimulatory effect of calponin. Ca(2+)-calmodulin affected neither the myosin-binding activity nor the stimulatory effect of calponin. We further presented a few data which suggest that calponin may exert regulatory activity toward myosin in quite a different way from caldesmon, another smooth muscle protein that binds to myosin, actin, and calmodulin.
authors.affiliation: Department of Pharmacology, Gunma University School of Medicine.
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
authors.forename: L H
authors.initials: LH
authors.lastname: Ye
authors.forename: R
authors.initials: R
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: K
authors.initials: K
authors.lastname: Kohama
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002148:Calmodulin-Binding Proteins / Q000494:pharmacology; D002645:Chickens; D066298:In Vitro Techniques; D007700:Kinetics; D008840:Microfilament Proteins; D009124:Muscle Proteins / Q000494:pharmacology; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology; D009132:Muscles / Q000187:drug effects / Q000201:enzymology; D009218:Myosins / Q000378:metabolism*; D000096985:Calponins
chemical_list: D017301:Ca(2+) Mg(2+)-ATPase; D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D009218:Myosins; D000096985:Calponins