title: Three monoclonal antibodies differentiate human from murine epidermis.
abstract: In order to establish a set of epidermal species markers, normal human skin, murine skin and human skin transplanted to nude mice were stained with monoclonal antibodies directed to cell membrane-bound carbohydrates, a basement membrane component and a structure in the cell nucleus. Three epidermal species markers were identified. Two markers stained exclusively human epidermis: LH7.2 detects type VII collagen and stained the basement membrane of human epidermis in unfixed frozen sections, while LP4N stained cell nuclei in human epidermis in methanol-acetone-fixed frozen sections. The third marker, HH14, stained exclusively murine epidermis. HH14 defines the histo-blood group H carbohydrate antigen and stained spinous cell membranes of murine epidermis in both frozen and formalin-fixed sections.
authors.affiliation: Department of Oral Diagnosis, Royal Dental College, Copenhagen, Denmark.
authors.forename: D
authors.initials: D
authors.lastname: Jeppe-Jensen
authors.forename: H
authors.initials: H
authors.lastname: Clausen
authors.forename: I M
authors.initials: IM
authors.lastname: Leigh
authors.forename: E B
authors.initials: EB
authors.lastname: Lane
authors.forename: E
authors.initials: E
authors.lastname: Dabelsteen
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002240:Carbohydrate Sequence; D004817:Epidermis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D006017:Glycolipids; D006023:Glycoproteins