title: Agr-related sequences in Staphylococcus lugdunensis.
abstract: Sequences related to the Staphylococcus aureus accessory gene regulator (agr) were demonstrated in S. lugdunensis by Southern blot analysis of 13 strains and sequencing of the S. lugdunensis agr-like locus (agr-sl). Northern blot analysis of cellular RNA revealed the presence of a transcript having homology with the agr-P3 transcript (RNAIII) for three of the six strains tested. The three strains containing this transcript produce a hemolysin with phenotypic properties similar to that of S. aureus delta-hemolysin. Nevertheless, unlike agr-P3 from S. aureus, agr-sl does not encode any potential peptide homologous to S. aureus delta-hemolysin, suggesting that the hemolytic activity detected in S. lugdunensis is encoded elsewhere and may be controlled by agr-sl.
authors.affiliation: Département de Recherche en Bactériologie Médicale, Lyon, France.
authors.forename: F
authors.initials: F
authors.lastname: Vandenesch
authors.forename: S J
authors.initials: SJ
authors.lastname: Projan
authors.forename: B
authors.initials: B
authors.lastname: Kreiswirth
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: R P
authors.initials: RP
authors.lastname: Novick
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006460:Hemolysin Proteins; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013210:Staphylococcus / Q000235:genetics* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C025994:delta hemolysin protein, Staphylococcus aureus; D004247:DNA