Observation of inter-subunit nuclear Overhauser effects in a dimeric protein. Application to the Arc repressor.
For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by double filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc repressor and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.
Burgering
M J
MJ
Bijvoet Center for Biomolecular Research, Utrecht, The Netherlands.
Boelens
R
R
Caffrey
M
M
Breg
J N
JN
Kaptein
R
R
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins; D009682:Magnetic Resonance Spectroscopy; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C060486:methionine repressor protein, Bacteria; C489220:methionine repressor protein, E coli; C016649:phage repressor proteins