title: Ruffles induced by Salmonella and other stimuli direct macropinocytosis of bacteria.
abstract: Ruffles are specialized plasma membrane ultrastructures of mammalian cells though to be integral to growth, development and locomotion. Induced by growth factors, mitogens or oncogene expression, ruffles are sites of filamentous actin rearrangement and are temporally associated with enhanced pinocytosis. But the function of ruffles, their mechanism of induction and their role in pinocytosis are not understood. We have observed formation of structures resembling ruffles associated with the site of entry of invasive Salmonella typhimurium. Here we report that ruffles elicited by invasive Salmonella directly mediate internalization of non-invasive bacteria in a macropinocytotic fashion, a phenomenon we term 'passive entry'. Furthermore, ruffles induced in the absence of Salmonella also facilitate passive entry. We present evidence that ruffles, common to many signalling events, comprise the macropinocytotic machinery mediating pinocytosis and are subverted by Salmonella so as to enter mammalian cells.
authors.affiliation: Department of Microbiology and Immunology, Stanford University, California 94305.
authors.forename: C L
authors.initials: CL
authors.lastname: Francis
authors.forename: T A
authors.initials: TA
authors.lastname: Ryan
authors.forename: B D
authors.initials: BD
authors.lastname: Jones
authors.forename: S J
authors.initials: SJ
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Falkow
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D002462:Cell Membrane / Q000382:microbiology / Q000502:physiology / Q000648:ultrastructure*; D066246:ErbB Receptors / Q000502:physiology; D004926:Escherichia coli / Q000502:physiology; D006801:Humans; D010873:Pinocytosis / Q000502:physiology*; D051381:Rats; D012486:Salmonella typhimurium / Q000472:pathogenicity / Q000502:physiology*; D014407:Tumor Cells, Cultured; D015011:Yersinia pseudotuberculosis / Q000502:physiology
chemical_list: D066246:ErbB Receptors