title: The effects of inhalation of grain dust extract and endotoxin on upper and lower airways.
abstract: To characterize the short-term effects of grain dusts on pulmonary function, mucosal inflammation, and systemic responses, four women and three men inhaled nebulized corn and soybean dust extracts, endotoxin diluted with Hanks' balanced salt solution (HBSS), and HBSS. Subjects were volunteers recruited via newspaper advertisement and were required to be healthy, nonasthmatic, nonatopic never-smokers. The mean age was 26.9 years (range, 19 to 36 years). Using a randomized, double-blind, crossover design, each subject was challenged with each of the 4 substances with at least 10 days between challenges. Serial spirometry, peripheral blood leukocyte and differential cell counts, and 24-h postchallenge nasal lavages were performed. Extracts were produced by mixing 3 g of the corn or soybean dust with 30 ml HBSS followed by shaking for 60 min, centrifugation, then filter sterilization. The endotoxin solution was produced by mixing lyophilized Escherichia coli endotoxin (serotype 0111:B4) with HBSS to attain a final concentration of 7 mg/L, which was the same as the concentration of endotoxin in both grain dust solutions. The pH of all solutions and unmixed HBSS was adjusted to 5.8, which was the native pH of the soybean dust extract. Subjects were challenged with 0.08 ml/kg of each substance, resulting in a range of endotoxin doses of 30 to 60 micrograms, similar to that which a worker might inhale over the course of one workshift. The lowest mean percentage baseline FEV1 (+/- SD) after inhalation challenge was 99.2 +/- 2.1 for HBSS, and it was significantly lower for endotoxin (90.1 +/- 8.5, p = 0.03), corn dust extract (93.1 +/- 4.3, p = 0.02), and soybean dust extract (96.2 +/- 3.7, p = 0.03). In addition, a peripheral blood leukocytosis developed after exposure to all three endotoxin-containing solutions (p < 0.05), yet a lower peripheral blood lymphocyte count was found only after inhalation of corn dust extract (p = 0.02). Interestingly, this was associated with a higher nasal lavage lymphocyte count after inhalation of corn dust extract (p = 0.03). Neither the decrease in peripheral blood lymphocytes nor the increase in nasal lymphocytes were found after inhalation of soybean dust extract or endotoxin. Our results indicate that extracts of grain dusts have physiologic effects similar to endotoxin. However, in spite of the same endotoxin levels, the effects of corn dust extract appear to have different biologic activity than either soybean dust extract or endotoxin.
authors.affiliation: Department of Internal Medicine, University of Iowa, Iowa City 52242.
authors.forename: W D
authors.initials: WD
authors.lastname: Clapp
authors.forename: P S
authors.initials: PS
authors.lastname: Thorne
authors.forename: K L
authors.initials: KL
authors.lastname: Frees
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: C R
authors.initials: CR
authors.lastname: Lux
authors.forename: D A
authors.initials: DA
authors.lastname: Schwartz
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D000336:Aerosols; D016084:Bronchoconstriction / Q000187:drug effects; D004311:Double-Blind Method; D004391:Dust / Q000009:adverse effects*; D004731:Endotoxins / Q000008:administration & dosage / Q000009:adverse effects*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D007552:Isotonic Solutions / Q000008:administration & dosage; D007958:Leukocyte Count; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D009297:Nasal Mucosa / Q000473:pathology; D013025:Glycine max; D013147:Spirometry; D014797:Vital Capacity; D003313:Zea mays
chemical_list: D000336:Aerosols; D004391:Dust; D004731:Endotoxins; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions