title: Postischemic (1 hour) hypothermia significantly reduces ischemic cell damage in rats subjected to 2 hours of middle cerebral artery occlusion.
abstract: OBJECTIVE
  We investigated the effect of hypothermia induced 1 hour after transient (2-hour) middle cerebral artery occlusion on the extent of ischemic cell damage in the rat.
  
  
  METHODS
  Middle cerebral artery occlusion was induced extracranially by insertion of a nylon filament into the right internal carotid artery. Two groups of rats were investigated: (1) rats (n = 10) subjected to normothermic (37 degrees C) ischemia and normothermic reperfusion; and (2) rats (n = 10) subjected to normothermic ischemia and 1 hour of normothermic reperfusion followed by 3 hours of hypothermia (30 degrees C). All rats were killed 1 week after the experiment, and brain sections were stained with hematoxylin and eosin for evaluation of ischemic cell damage.
  
  
  RESULTS
  Infarct volume in normothermic rats involved 20.9 +/- 4.6% of the hemisphere, whereas hypothermic rats exhibited a significantly smaller (P < .001) infarct volume of 11.1 +/- 2.7%. The numbers of surviving (or structurally intact) neurons within large sections of the cortex and striatum were significantly greater for hypothermic compared with normothermic rats (P < .01).
  
  
  CONCLUSIONS
  Our data suggest that postischemic induction of hypothermia significantly reduces ischemic cell damage after 2 hours of middle cerebral artery occlusion in the rat, and that an interval of time of at least 1 hour after ischemia exists in which hypothermic intervention is effective in either salvaging or postponing irreversible neuronal injury.
authors.affiliation: Department of Neurology, Henry Ford Hospital, Detroit, MI 48202.
authors.forename: R L
authors.initials: RL
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Chopp
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: J H
authors.initials: JH
authors.lastname: Garcia
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001831:Body Temperature; D001921:Brain / Q000473:pathology / Q000503:physiopathology; D002545:Brain Ischemia / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D007036:Hypothermia, Induced; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012007:Rectum / Q000503:physiopathology