title: A strong-stop DNA in rice plants infected with rice tungro bacilliform virus.
abstract: A virus-specific small nucleic acid (strong-stop DNA) was identified in rice plants infected with rice tungro bacilliform virus, but not in the virus particles. This nucleic acid was shown to consist of about 595 deoxyribonucleotides with about 70 ribonucleotides covalently linked at the 5' end. Hybridization with sequence-specific oligonucleotides showed that the ribonucleotides were from the plant cytoplasmic tRNA(iMet) sequence. PCR analysis detected hairpin structures at the 3' end of the DNA.
authors.affiliation: Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, U.K.
authors.forename: Y
authors.initials: Y
authors.lastname: Bao
authors.forename: R
authors.initials: R
authors.lastname: Hull
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D009690:Nucleic Acid Conformation; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D010942:Plant Viruses / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012358:RNA, Transfer, Met / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012358:RNA, Transfer, Met