title: The gdhA1 point mutation in Escherichia coli K12 CLR207 alters a key lysine residue of glutamate dehydrogenase.
abstract: gdhA1 is a spontaneous mutant of Escherichia coli that causes complete loss of activity of the NADP-specific glutamate dehydrogenase (GDH) encoded by the gdhA gene. The gdhA1 mutational site has been identified by recombinational mapping, polymerase chain reaction (PCR) amplification and DNA sequencing, as an A to G transition at nucleotide 274 of the gdhA coding sequence, resulting in an amino acid change of lysine 92 to glutamic acid. The mutant enzyme forms hybrid hexamers with a wild-type GDH, providing a useful system for analysis of conformational integrity of mutational variants.
authors.affiliation: Department of Genetics, University of Leeds, UK.
authors.forename: K M
authors.initials: KM
authors.lastname: Jones
authors.forename: M J
authors.initials: MJ
authors.lastname: McPherson
authors.forename: A J
authors.initials: AJ
authors.lastname: Baron
authors.forename: I W
authors.initials: IW
authors.lastname: Mattaj
authors.forename: C L
authors.initials: CL
authors.lastname: Riordan
authors.forename: J C
authors.initials: JC
authors.lastname: Wootton
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005969:Glutamate Dehydrogenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008239:Lysine / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011995:Recombination, Genetic; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D005969:Glutamate Dehydrogenase; D008239:Lysine