Injury to cultured liver endothelial cells during cold preservation: energy-dependent versus energy-deficiency injury.
Previously, we demonstrated an energy-dependent injury to cultured liver endothelial cells during cold incubation in University of Wisconsin (UW) solution. Here, the effects of Histidine-Tryptophan-Ketoglutarate (HTK) and Euro-Collins (EC) solutions on these cells were studied. In HTK solution, 83% +/- 4% of the cells had lost viability after 9 h of incubation at 4 degrees C. The addition of cyanide (1 mM) to simulate hypoxic conditions protected the cells to the extent that only 9% +/- 1% of the cells lost viability over the same period; the addition of glucose (10 mM) led to increased cell injury. ATP levels were highest in the incubations with the most rapid loss of viability. In Krebs-Henseleit buffer and EC solution, in contrast, cell injury increased upon addition of cyanide; the addition of glucose to Krebs-Henseleit buffer decreased injury. We conclude that the injury to cultured liver endothelial cells during cold incubation in HTK solution is energy-dependent, as it is in UW solution, whereas cells behave differently in EC solution and Krebs-Henseleit buffer.
Rauen
U
U
Klinische Forschergruppe Leberschädigung, Heinrich-Heine-Universität, Düsseldorf, Germany.
Hintz
K
K
Hanssen
M
M
Lauchart
W
W
Becker
H D
HD
de Groot
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002021:Buffers; D002470:Cell Survival; D002478:Cells, Cultured; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000009:adverse effects*; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000293:injuries; D005947:Glucose / Q000494:pharmacology; D006982:Hypertonic Solutions / Q000009:adverse effects; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000293:injuries*; D008297:Male; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D002021:Buffers; D003451:Cryoprotective Agents; D006982:Hypertonic Solutions; D000255:Adenosine Triphosphate; D005947:Glucose; D011190:Potassium Cyanide