Comparative studies of intracellular activity, secretion and multiple forms spectra of human skin fibroblast alpha-L-fucosidase in the normaland after sucrose load.
Activity and multiple forms of intracellular and secreted alpha-L-fucosidases from human skin fibroblasts were studied in normal and sucrose-loaded cells--modelling intralysosomal nonhydrolyzable products' accumulation in vitro. Certain differences in secretion levels were found after sucrose load for alpha-L-fucosidases from embryonal and postnatal cell lines. Different effects of sucrose load on multiple forms' spectra of the intracellular and secreted alpha-L-fucosidases from embryonal and postnatal fibroblasts were revealed.
Beyer
E M
EM
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow.
Ivleva
T S
TS
Artykova
G T
GT
Wiederschain GYa
D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D012867:Skin / Q000201:enzymology*; D013395:Sucrose / Q000494:pharmacology*; D005644:alpha-L-Fucosidase / Q000302:isolation & purification / Q000378:metabolism*
D007527:Isoenzymes; D013395:Sucrose; D005644:alpha-L-Fucosidase