Toxicity of bile acids to colon cancer cell lines.
Quantitative aspects of bile acid cytotoxicity to colon cancer cell lines were investigated because of the etiological role in colon carcinogenesis attributed to the toxic effects of bile acids on colon mucosal cells. The cytotoxicity of major colonic bile acids differed. Lithocholate was the most toxic, followed by chenodeoxycholate and deoxycholate, with cholate being non-toxic over the concentration range studied. Cytotoxicity increased with time of exposure. Values for IC50 for some of the acids were determined to be in the physiological range, as estimated from their concentrations in fecal water. The results suggest dietary factors that contribute to bile acid mucosal damage. They also identify factors of possible importance in the association of high concentrations of bile acids in fecal water with risk for colon cancer.
Latta
R K
RK
Institute for Biological Sciences, National Research Council of Canada, Ottawa.
Fiander
H
H
Ross
N W
NW
Simpson
C
C
Schneider
H
H
D001647:Bile Acids and Salts / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002635:Chenodeoxycholic Acid / Q000633:toxicity; D003110:Colonic Neoplasms / Q000473:pathology*; D003840:Deoxycholic Acid / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000737:chemistry; D006801:Humans; D016018:Least-Squares Analysis; D008095:Lithocholic Acid / Q000633:toxicity; D012710:Serum Albumin, Bovine; D013997:Time Factors; D014407:Tumor Cells, Cultured
D001647:Bile Acids and Salts; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D012710:Serum Albumin, Bovine; D008095:Lithocholic Acid