The ribosome-inactivating, antiproliferative and teratogenic activities and immunoreactivities of a protein from seeds of Luffa aegyptiaca (Cucurbitaceae).
1. The protein isolated from Luffa aegyptiaca seeds was capable of inhibiting protein synthesis in a rabbit reticulocyte lysate system and [3H]thymidine uptake by mouse melanoma (B16) cells. 2. It also adversely affected the development of mouse embryos in culture. 3. In enzyme-linked immunosorbent assay it reacted with antisera raised against other ribosome-inactivating proteins.
Ng
T B
TB
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.
Chan
W Y
WY
Yeung
H W
HW
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002474:Cell-Free System; D005314:Embryonic and Fetal Development / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D066298:In Vitro Techniques; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010940:Plant Proteins / Q000276:immunology / Q000494:pharmacology* / Q000633:toxicity; D011247:Pregnancy; D011500:Protein Synthesis Inhibitors / Q000276:immunology / Q000494:pharmacology* / Q000633:toxicity; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects*; D012639:Seeds / Q000737:chemistry*; D013723:Teratogens / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D013723:Teratogens