Molecular genetic analysis of beta-toxin of Clostridium perfringens reveals sequence homology with alpha-toxin, gamma-toxin, and leukocidin of Staphylococcus aureus.
Oligonucleotide probes designed on the basis of the N-terminal sequence of Clostridium perfringens beta-toxin were used to isolate the encoding gene (cpb). The nucleotide sequence of cpb was determined, and on the basis of DNA hybridization experiments it was shown that the gene is found only in type B and C strains of C. perfringens. The deduced amino acid sequence of the beta-toxin revealed homology with the alpha-toxin, gamma-toxin, and leukocidin of Staphylococcus aureus. The beta-toxin purified from C. perfringens appeared to exist in monomeric and multimeric forms. Recombinant beta-toxin, produced in Escherichia coli, appeared to be mainly in the multimeric form.
Hunter
S E
SE
Chemical and Biological Defence Establishment, Salisbury, Wiltshire, United Kingdom.
Brown
J E
JE
Oyston
P C
PC
Sakurai
J
J
Titball
R W
RW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003016:Clostridium perfringens; D004926:Escherichia coli / Q000235:genetics; D006460:Hemolysin Proteins / Q000737:chemistry*; D007956:Leukocidins / Q000737:chemistry*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C031884:CPB protein, Clostridium perfringens; D006460:Hemolysin Proteins; D007956:Leukocidins; D015345:Oligonucleotide Probes; C037956:gamma-hemolysin, Staphylococcus aureus; C021091:staphylococcal alpha-toxin