[Creation of a hybrid protein gene based on Bacillus thuringiensis delta endotoxins CryIIIA and CrIA(a) and expression of its derivatives in Escherichia coli].
The 5'-terminal fragment (containing 1-565th codons) of Bacillus thuringiensis var. tenebrionis gene for the Coleoptera-specific delta-endotoxin CryIIIA was cloned. This sequence was extended with either only a homologous fragment of CryIA(a) or that one together with in-frame NPTII or GUS coding sequences. The obtained gene derivatives were expressed in E. coli. The analysis of hybrid polypeptides confirmed the enzymatic activities of bifunctional proteins and showed toxic properties of "insectotoxin-NPTII" against Colorado potato beetle (Leptinotarsa decemlineata).
Shadenkov
A A
AA
Kadyrov
R M
RM
Uzbekova
S V
SV
Kuz'min
E V
EV
Osterman
A L
AL
Chestukhina
G G
GG
Shemiakin
M F
MF
D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D001483:Base Sequence; D004274:DNA, Recombinant; D004731:Endotoxins / Q000235:genetics*; D004926:Escherichia coli; D015870:Gene Expression; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004274:DNA, Recombinant; D004731:Endotoxins; D006460:Hemolysin Proteins; D011993:Recombinant Fusion Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Sozdanie gena gibridnogo belka na osnove delta-éndotoksinov Bacillus thuringiensis CryIIIA i CryIA(a) i ékspressiia ego proizvodnykh v Escherichia coli.