title: Cardioplegic effect of University of Wisconsin solution on hypothermic ischemia of rat myocardium assessed by mitochondrial oxidative phosphorylation.
abstract: The effectiveness of the University of Wisconsin solution and the Collins' M solution for preservation of rat hearts was compared by examining histologic appearance, tissue water content, and mitochondrial respiratory functions after prolonged hypothermic storage and subsequent heterotopic transplantation. Survival of transplanted hearts after 5 days of reperfusion was markedly lowered by storage in Collins' M solution for 15 hours. Hearts stored in University of Wisconsin solution for 10 hours showed no increase in myocardial necrosis after 5 days of reperfusion, whereas hearts stored in University of Wisconsin solution for 15 hours and Collins' M solution for 10 and 15 hours showed a significant increase in tissue necrosis. University of Wisconsin solution reduced tissue swelling during hypothermic storage, whereas Collins' M solution did not cause such reduction. The yield of mitochondrial protein after reperfusion was significantly decreased by storage in either solution, especially after 15 hours in Collins' M solution. Mitochondrial oxidative phosphorylation was significantly inhibited by storage, especially by storage in Collins' M solution and subsequent reperfusion. These results indicate that myocardial injury, after prolonged ischemia and reperfusion, results in a decrease in functionally and structurally intact mitochondria that is dependent on preservation conditions. University of Wisconsin solution protects isolated hearts against ischemia and reperfusion injury possibly by preventing cellular and mitochondrial deterioration.
authors.affiliation: Second Department of Surgery, Miyazaki Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yano
authors.forename: H
authors.initials: H
authors.lastname: Takenaka
authors.forename: T
authors.initials: T
authors.lastname: Onitsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Koga
authors.forename: M
authors.initials: M
authors.lastname: Hamada
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001834:Body Water / Q000378:metabolism; D002314:Cardioplegic Solutions; D003080:Cold Temperature; D005978:Glutathione; D006085:Graft Survival; D016027:Heart Transplantation; D006982:Hypertonic Solutions; D007328:Insulin; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D009336:Necrosis; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010085:Oxidative Phosphorylation; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D002314:Cardioplegic Solutions; C024102:Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose