Leucocyte endothelial cell adhesion: a study comparing human umbilical vein endothelial cells and the endothelial cell line EA-hy-926.
EA-hy-926 is a cell line produced by hybridizing human umbilical vein endothelial cells (HUVEC) and the epithelial cell line A549. To establish whether EA-hy-926 could be used as a model for endothelial cells (EC) in leucocyte-EC adhesion interactions, the effect of interleukin-4 (IL-4), tumour necrosis factor (TNF) or interferon-gamma (IFN) stimulation on their adhesiveness and expression of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) was compared with that of HUVEC and A549. Although HUVEC exhibited increased adhesiveness and adhesion molecule expression with IL-4, TNF or IFN, EA-hy-926 exhibited these responses only with TNF. CD11/CD18-dependent binding accounted for a significant component of basal binding to HUVEC and EA-hy-926, but did not account for the increased binding of T cells, JY, J6, ICH-BJ or ICH-KM cell lines to TNF-stimulated monolayers. At least part of the CD11/CD18-independent adhesion was attributable to VCAM-1 induction on HUVEC and EA-hy-926. TNF-stimulation also induced E-selectin expression on EA-hy-926 and HUVEC and an accompanying increase in neutrophil (PMN) binding. The EA-hy-926 cells used in this study, therefore, showed responses similar to HUVEC when stimulated with TNF but not when stimulated with IL-4 or IFN.
Thornhill
M H
MH
Department of Oral Medicine and Periodontology, London Hospital Medical College, UK.
Li
J
J
Haskard
D O
DO
D001402:B-Lymphocytes / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D016207:Cytokines / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D007962:Leukocytes / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology; D013601:T-Lymphocytes / Q000502:physiology; D014471:Umbilical Veins / Q000166:cytology
D016207:Cytokines