title: [Newly developed method by the use of factor Y assay for the detection of the lupus anticoagulant].
abstract: A New method for the detection of lupus anticoagulant was developed. Plasma factor V activities using tissue thromboplastin (simplastin auto-SA) and partial thromboplastin (platelin excel LS-LS) were measured simultaneously. Furthermore, the ratio of the two activities was calculated (SA/LS ratio) as a marker of lupus anticoagulant. The normal range of SA/LS ratio was 0.79-1.39 (mean +/- 3SD). The high SA/LS ratios were detected in all of 15 patients with positive lupus anticoagulant, in 3 (5.8%) of 52 SLE patients with normal activated partial thromboplastin time, in 2 (28.7%) of 7 patients with hemophilia, in 1 (33.3%) of 3 patients with factor VIII inhibitor and in 4 (3.3%) of 122 patients with various diseases, respectively. All of 15 patients with liver dysfunction, 54 patients under warfarin treatment and 8 patients under heparin treatment had normal SA/LS ratio.
authors.affiliation: Department of Laboratory Medicine, Gunma University, School of Medicine, Maebashi.
authors.forename: H
authors.initials: H
authors.lastname: Amagai
authors.forename: R
authors.initials: R
authors.lastname: Shizuka
authors.forename: M
authors.initials: M
authors.lastname: Hayakawa
authors.forename: J
authors.initials: J
authors.lastname: Kojima
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumura
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: I
authors.initials: I
authors.lastname: Kobayashi
mesh_terms: D005165:Factor V / Q000032:analysis*; D005260:Female; D006467:Hemophilia A / Q000097:blood; D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000097:blood*; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D008297:Male; D008722:Methods; D012016:Reference Values
chemical_list: D016682:Lupus Coagulation Inhibitor; D005165:Factor V