Seasonal enhancement of IL-4 induced IgE synthesis by peripheral blood mononuclear cells of atopic patients.
The role of IL-4 on IgE synthesis has been well established. IL-4 has been shown to promote IgE production by B cells from atopic and non-atopic donors. In this study, the effects of natural exposure to pollens on IL-4-induced IgE synthesis by peripheral blood mononuclear cells of atopic and non-atopic donors were examined. The results confirm production of IgE in an IL-4 dose-dependent manner by PBMC cultures of these two groups. When cultures were performed out of the pollen season, following stimulation by IL-4, no significant differences was observed between the levels of IgE produced by PBMC of atopic and non-atopic donors. In contrast, upon natural exposure to pollens, significant higher levels of IgE were measured in the atopic group than in the non-atopic one. These results show that the pollen season influences the IL-4-induced IgE synthesis by PBMC of allergic patients and are in keeping with seasonal rise of specific IgE antibodies.
Gagnon
R
R
Centre de Recherche en Inflammation, Immunologie et Rhumatologie, Centre de Recherche du Centre Hospitalier de l'Université Laval, Ste-Foy, Québec, Canada.
Lian
J
J
Boutin
Y
Y
Hébert
J
J
D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000097:blood*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000494:pharmacology*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D006109:Poaceae; D011058:Pollen; D012016:Reference Values; D012621:Seasons
D015847:Interleukin-4; D007073:Immunoglobulin E