Functional complementation in mouse-human radiation hybrids assigns the putative murine scid gene to the pericentric region of human chromosome 8.
Fragments of human chromosome 8 were introduced into cells derived from murine scid mice via X-irradiation and somatic cell fusion. The resulting hybrid clones contained human DNA fragment(s) which complemented the hyper-radiosensitivity of the scid cells. Alu-PCR products from these hybrids were used for chromosome painting using the technique of chromosome in situ suppression hybridization, allowing assignment of the human HYRC (hyper-radiosensitivity of murine scid mutation, complementing) gene, a candidate for a V(D)J recombinase gene, to human chromosome 8q11.
Komatsu
K
K
Department of Radiation Biophysics, Nagasaki University School of Medicine, Japan.
Ohta
T
T
Jinno
Y
Y
Niikawa
N
N
Okumura
Y
Y
D000818:Animals; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D005816:Genetic Complementation Test; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016513:Mice, SCID / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics; D013045:Species Specificity; D045567:VDJ Recombinases
D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases