Production of a monoclonal antibody inhibiting the killer activity.
We established the hybridoma producing the monoclonal antibody (mAb) 3C3 by immunizing rats with mouse natural killer (NK)-like cells. The 3C3 mAb seemed to react mainly with T cells and T-lineage cell lines. The 3C3 antigen also seemed to be coincidentally expressed on a part of asialo GM1+ cells from nude mice, suggesting its expression on NK cells. Treatment of effector cells with 3C3 mAb markedly inhibited the killer activity against RL male-1 cells, but less so against YAC-1 cells, in vitro. It is suggested that the cell surface molecule defined by 3C3 mAb was closely associated with the killer activity of T cells and NK cells.
Suzuki
Y
Y
Laboratory of Bacteriology, Aichi Prefectural Institute of Public Health, Japan.
Funabashi
M
M
Suzuki
R
R
Notake
K
K
Yokochi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005677:G(M1) Ganglioside / Q000032:analysis; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008819:Mice, Nude / Q000276:immunology; D051381:Rats; D011916:Rats, Inbred F344 / Q000276:immunology; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside