Spleen-derived growth factor, SDGF-3, is identified as keratinocyte growth factor (KGF).
A heparin-binding mitogen rat for rat hepatocytes was partially purified from bovine spleen by a combination of heparin-affinity, cation-exchange and gel-filtration chromatography. Besides stimulating rat hepatocytes, this factor, which was designated spleen-derived growth factor-3 (SDGF-3), exhibited mitogenic activity for mouse epidermal keratinocytes but not mouse fibroblasts. Its apparent epithelial specificity and heparin-binding properties corresponded to those of keratinocyte growth factor (KGF). These findings, together with the fact that the mitogenic activity of SDGF-3 was abolished by a neutralizing monoclonal antibody specific for KGF, identify this bovine spleen-derived hepatocyte mitogen as KGF.
Suzuki
M
M
Cell Biology Laboratory, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
Itoh
T
T
Osada
H
H
Rubin
J S
JS
Aaronson
S A
SA
Suzuki
T
T
Koga
N
N
Saito
T
T
Mitsui
Y
Y
D016475:3T3 Cells; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006493:Heparin / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000737:chemistry
C496454:Fgf7 protein, mouse; C496455:Fgf7 protein, rat; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D011994:Recombinant Proteins; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D006493:Heparin; D004247:DNA