Application of solid-phase partition cartridges in the determination of fungicide residues in vegetable samples.
Disposable, ready-to-use cartridges filled with a macroporous diatomaceous material are used to extract in a single step fungicide residues with dichloromethane from aqueous acetone extracts of vegetables. This procedure takes the place of some functions (such as separating funnel partition, drying over anhydrous sodium sulphate and clean-up) usually performed by separate steps in classical schemes. Fourteen fungicides (dichloran, vinclozolin, chlorthalonil, triadimefon, dichlofluanide, procymidone, hexaconazole, captan, folpet, ditalimfos, iprodione, captafol, pyrazophos and fenarimol) were determined using the described procedure with recoveries between 83 and 107% at spiking levels ranging for the different compounds from 0.04 to 0.40 mg/kg. Crops subjected to the described procedure included lettuce, strawberry, apple, yellow pepper and peach, and gave extracts containing a mass of co-extractives between 5 and 30 mg. Compared with classical schemes, the described procedure is simple, less labour intensive, allows parallel handling of several extracts and does not require preparation and maintenance of equipment. Troublesome emulsions such as those frequently observed in separating funnel partitioning do not occur.
Di Muccio
A
A
Laboratorio di Tossicologia Applicata, Istituto Superiore di Sanità, Rome, Italy.
Dommarco
R
R
Attard Barbini
D
D
Santilio
A
A
Girolimetti
S
S
Ausili
A
A
Ventriglia
M
M
Generali
T
T
Vergori
L
L
D002849:Chromatography, Gas / Q000379:methods*; D007692:Diatomaceous Earth; D005506:Food Contamination; D005638:Fruit / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
D005659:Fungicides, Industrial; D010573:Pesticide Residues; D007692:Diatomaceous Earth