The relationship between induction of peroxisomal beta-oxidation and long-chain fatty acid composition in rat liver.
The long-chain fatty acid composition in rat liver, by which peroxisomal beta-oxidation system was induced, was analyzed in vitro and in vivo. In primary culture of hepatocytes, treatment with 0.4 mM clofibrate or long-chain fatty acids caused an increase in the peroxisomal beta-oxidation activity, in addition the oleic acid [18:1(n-9)] content in the cells was increased two-fold by addition of very long-chain fatty acids to the culture medium, but clofibrate had no effect. A two-fold of increase in 18:1(n-9) content was observed in the hepatic subcellular fractions from rats fed a diet containing 0.25% clofibric acid. The ratio of cis-vaccenic acid [18:1(n-7)] to total 18:1 content decreased by more than half compared with control in clofibric acid treated liver. The level of 18:1(n-7) was not changed in all experiments. It is suggested that perturbation of C-18 chain length fatty acids occurs in peroxisomal beta-oxidation induced liver.
Kawano
H
H
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
Shibahara
A
A
Yamamoto
K
K
Hama
T
T
Kajimoto
G
G
D000818:Animals; D001823:Body Composition / Q000502:physiology; D002994:Clofibrate / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D005227:Fatty Acids; D002994:Clofibrate