title: Contractile protein gene expression in serum-free cultured adult rat cardiac myocytes.
abstract: The effects of two adhesion substrates (serum and laminin) and time in culture on the expression of genes encoding myosin heavy chain (MHC) isoforms and alpha-skeletal actin were analysed in myocytes isolated from adult rat heart and maintained in serum-free culture. Relative messenger ribonucleic acid (mRNA) abundances were quantitated by dot-blot analysis. Gene expression was not influenced by the substrate used. Time in culture induced a decrease in total mRNA abundance and an up-regulation of beta-MHC and alpha-skeletal actin genes. It is proposed that atrophy of adult myocytes is associated with a pattern of gene expression similar to the fetal program.
authors.affiliation: Unité 127 INSERM, Hôpital Lariboisière, Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Dubus
authors.forename: L
authors.initials: L
authors.lastname: Rappaport
authors.forename: A
authors.initials: A
authors.lastname: Barrieux
authors.forename: A M
authors.initials: AM
authors.lastname: Lompré
authors.forename: K
authors.initials: K
authors.lastname: Schwartz
authors.forename: J L
authors.initials: JL
authors.lastname: Samuel
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002448:Cell Adhesion; D003285:Contractile Proteins / Q000096:biosynthesis* / Q000235:genetics; D016895:Culture Media, Serum-Free; D004247:DNA; D015342:DNA Probes; D015870:Gene Expression; D017403:In Situ Hybridization; D007797:Laminin / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000096:biosynthesis / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000502:physiology
chemical_list: D000199:Actins; D003285:Contractile Proteins; D016895:Culture Media, Serum-Free; D015342:DNA Probes; D007797:Laminin; D015347:RNA Probes; D012333:RNA, Messenger; D004247:DNA; D009218:Myosins