[Effect of surface antigens on granulocytes in phagocytosis].
To analyze phagocytosis mechanism of granulocyte using fluorescent microspheres, changes of phagocytic index, complement receptors, Fc receptors and adhesion molecules on cell membrane, after exogenous stimulation were determined by flow cytometry. The changes of phagocytic index by blocking test was also determined using monoclonal antibodies for receptors. The phagocytic index was decreased by supernatant of S. aureus, and was increased by supernatant of E. coli and phorbol myristate acetate (PMA). By PMA stimulation, surface marker positive cells increased in CR1, IgGFcR2 and decreased in IgGFcR3, LFA-3, Homing receptor, whereas they increased in CR1, IgMFcR, LFA-1 beta and decreased in LFA-3 by the supernatants of S. aureus and E. coli. Blocking test using monoclonal antibodies to individual receptors showed decrease of phagocytic index in CR1, CR3, LFA-1 beta. In this phagocytosis using fluorescent microspheres, the complement receptors were most dedicating in phagocytosis of granulocyte.
Sakai
H
H
Department of Clinical Laboratory, National Cardiovascular Center Hospital, Suita.
Mori
K
K
Katayama
Y
Y
Matsuyama
T
T
D000328:Adult; D000954:Antigens, Surface / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D010587:Phagocytosis / Q000276:immunology*; D011951:Receptors, Complement / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D011951:Receptors, Complement; D011961:Receptors, Fc