title: Expression of alkaline phosphatase in the mature mouse placenta visualized by in situ hybridization and enzyme histochemistry.
abstract: Alkaline phosphatases (APs) are a family of cell surface glycoproteins that are expressed in a variety of tissues. Their physiological functions are still unclear. Three different AP genes have been found to be expressed in mice, and AP cloned from the placenta is of the tissue non-specific (TNAP) type. We have in investigated the location of TNAP mRNA and active AP in mature mouse placenta, using in situ hybridization and enzyme histochemistry on serial sections. Digital image analysis was used to estimate relative amounts of TNAP mRNA. Tissue non-specific alkaline phosphatase messenger was detected only in the placental labyrinth, whereas active AP was present both in the labyrinth and in a zone of cells at the margin of the decidua basalis, bordering the myometrium and the metrial gland. This latter location of AP activity has not been described previously. The AP-positive zone of the decidua had a condensed appearance and a central defect in the zone was visible on sections taken from the middle of the placenta. No TNAP messenger was found in the zone of AP-positive decidual cells.
authors.affiliation: Department of Zoology, Uppsala University, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Johansson
authors.forename: M
authors.initials: M
authors.lastname: Wide
authors.forename: E
authors.initials: E
authors.lastname: Young
authors.forename: P
authors.initials: P
authors.lastname: Lindblad
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D003656:Decidua / Q000201:enzymology; D005260:Female; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D010920:Placenta / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D000469:Alkaline Phosphatase