Rapid binding of synapsin I to F- and G-actin. A study using fluorescence resonance energy transfer.
Synapsin I is a nerve terminal phosphoprotein which interacts with synaptic vesicles and actin in a phosphorylation-dependent manner. By using fluorescence resonance energy transfer between purified components labeled with fluorescent probes, we now show that the binding of synapsin I to actin is a rapid phenomenon. Binding of synapsin I to actin can also be demonstrated when synaptic vesicles are present in the medium and appears to be modulated by ionic strength and synapsin I phosphorylation.
Ceccaldi
P E
PE
Dept. of Medical Pharmacology, University of Milan, Italy.
Benfenati
F
F
Chieregatti
E
E
Greengard
P
P
Valtorta
F
F
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D013050:Spectrometry, Fluorescence; D016704:Synapsins / Q000378:metabolism*
D000199:Actins; D016704:Synapsins