[Participation of procoagulant activity of cultured rabbit lens epithelial cells in fibrin formation].
To clarify the participation of the lens epithelial cells in the postoperative fibrin formation in cataract surgery, the generation of procoagulant activity (PCA) by cultured rabbit lens epithelial (RLE) cells was investigated. PCA was detected in the homogenates of RLE cells cultured for three hours. PCA was increased approximately three- to four-fold by lipopolysaccharide (LPS) stimulation in a dose dependent manner. Treatment of the RLE cells with Concanavalin A resulted in a decrease of PCA. PCA of the RLE cells was not observed in Factor VII deficient plasma, as a standard tissue factor (TF). Parallelism was recognized between the dose response regression lines for the PCA in the RLE cells and standard TF. These results suggest that the PCA in the RLE cells is similar to TF, which has glycoprotein as an active site and requires coagulation factor VII. Some arachidonic acid metabolic cascade inhibitors which show an inhibitory effect for fibrin formation in vivo reduced the generation of PCA by the RLE cells in vitro. The activation of arachidonic acid cascade followed by the generation of PCA in the RLE cells may participate in postoperative fibrin formation.
Takahashi
K
K
Department of Bacteriology, School of Medicine, Iwate Medical University, Morioka, Japan.
D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005337:Fibrin / Q000096:biosynthesis*; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D013925:Thromboplastin / Q000378:metabolism
D005337:Fibrin; D013925:Thromboplastin