title: ADP-ribosylation of nucleolar proteins in HeLa tumor cells.
abstract: ADP-ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with 32P-NAD; then the nucleolar proteins were analyzed by 1-dimensional and 2-dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins were detected by autoradiography. The labeled nucleolar proteins were also chromatographically fractionated on DEAE-cellulose. Electrophoretic analysis of total nucleolar and chromatographically purified proteins revealed that besides nuclear ADP-ribosyltransferase and histones two characteristic nucleolar phosphoproteins numatrin/B23 and nucleolin/C23 were modified by ADP-ribosylation.
authors.affiliation: Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
authors.forename: N
authors.initials: N
authors.lastname: Leitinger
authors.forename: J
authors.initials: J
authors.lastname: Wesierska-Gadek
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D002455:Cell Division; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D000246:Adenosine Diphosphate Ribose