An improved method for perforated patch recordings using nystatin-fluorescein mixture.
This paper describes an improved method for solubilizing nystatin in an aqueous solution without the aid of organic solvents. The patch pipette was filled with a solution of nystatin and fluorescein sodium mixed in a molar ratio of 1:10. The success rate of perforated patch recordings was substantially improved, and the access resistance of 20-40 M omega was readily achieved.
Yawo
H
H
Department of Physiology, Kyoto University Faculty of Medicine, Japan.
Chuhma
N
N
D000200:Action Potentials; D000818:Animals; D002642:Chick Embryo; D004594:Electrophysiology / Q000379:methods*; D005069:Evaluation Studies as Topic; D019793:Fluorescein; D005452:Fluoresceins; D008564:Membrane Potentials; D009474:Neurons / Q000502:physiology; D009761:Nystatin; D012996:Solutions
D005452:Fluoresceins; D012996:Solutions; D009761:Nystatin; D019793:Fluorescein