DNA sequence analysis of serologically detected ELA class II haplotypes at the equine DQ beta locus.
The genetic diversity at the ELA DQ beta locus was investigated using polymerase chain reaction and DNA sequencing. Based upon serological methods 16 class II homozygous animals were selected and their genomic DNA was used. A DQ beta gene from an equine cDNA library was also sequenced. Our methodology and the similarity between the genomic and the cDNA sequences suggest that the studied locus is expressed on equine lymphocytes. In the predicted amino acid sequence the most extensive variation is located at residues 56-60. The pattern of these five amino acids is strongly correlated to the serological ELA class II specificities (W13, W22, W23, Be200). The alleles corresponding to the W23 specificity are the most divergent among the equine DQ beta alleles and also from other mammalian DQ beta sequences.
Szalai
G
G
Division of Immunogenetics, School of Veterinary Medicine, University of Berne, Switzerland.
Bailey
E
E
Gerber
H
H
Lazary
S
S
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015723:Gene Library; D005802:Genes, MHC Class II / Q000235:genetics*; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000235:genetics*; D006736:Horses / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D000949:Histocompatibility Antigens Class II; D004247:DNA