Modulation by drugs acting on the autonomic nervous system of platelet-activating factor in the stomach of rats.
Platelet-activating factor (PAF), an ether linked choline glycerophospholipid, is a potent initiator of diverse physiological and pathological processes. We have reported that gastric endogenous PAF levels were reduced and the contents of each of its molecular species changed during water-immersion stress in rats (Sugatani J et al., FASEB J 3: 65-70, 1989 and Sugatani J et al., Lipids 26: 1347-1353, 1991). In this study, we determined the effects of autonomic drugs on the level of gastric PAF, its molecular heterogeneity and formation of gastric erosions in unstressed rats and those subjected to water-immersion stress. Atropine, an anticholinergic drug, suppressed both the stress-induced changes and development of gastric lesions. 6-Hydroxydopamine-induced sympathectomy induced a small decrease in the gastric PAF levels and the addition of stress further decreased the PAF levels and development of gastric lesions. Carbamylcholine induced a transient decrease in the gastric PAF level of normal rats, which was not associated with gastric erosion formation. In contrast, the endogenous gastroprotective factor dopamine evoked transient dose- and time-dependent increases in the gastric PAF levels. These observations indicate that cholinergic muscarinic-receptor activation in rats led to decreases in gastric PAF levels and a prolonged and marked decrease in its level was associated with the development of gastric lesions, and that dopamine increases gastric PAF levels. Gastric endogenous PAF levels are closely associated with the autonomic nervous system and should be considered further in investigations of gastric function.
Sugatani
J
J
Department of Medical Chemistry, Kansai Medical University, Osaka, Japan.
Miwa
M
M
Fujimura
K
K
Saito
K
K
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001337:Autonomic Agents / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology; D002395:Catecholamines / Q000032:analysis; D004298:Dopamine / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D016627:Oxidopamine / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000378:metabolism
D001337:Autonomic Agents; D002395:Catecholamines; D010972:Platelet Activating Factor; D001285:Atropine; D016627:Oxidopamine; D002217:Carbachol; D004298:Dopamine