Action of alpha-dendrotoxin on K+ currents in nerve terminal regions of axons in rat olfactory cortex.
1. In the rat olfactory cortex, unmyelinated axons give rise to synapses en passant. This tissue was used to study the pharmacology of axonal K(+)-currents. Responses were measured from a group of these axons as unclamped field currents, with a polarizable suction electrode. 2. A single stimulus to the axons elicited a tetrodotoxin-sensitive Na(+)-dependent transient K(+)-currents were revealed by positive polarization of the suction electrode and were manifest as a negative current following the Na(+)-component. 3. In the presence of tetraethylammonium (TEA, 5 mM) and Cd2+ (100 microM), the K(+)-component was depressed by 3,4-diaminopyridine (3,4-DAP; 1 to 20 microM; IC50 2.0 +/- 0.4 microM). alpha-Dendrotoxin (DTX; 15-1500 nM) also attenuated the aminopyridine-sensitive component (IC50 93 +/- 4 nM). At the highest DTX concentration, depression of the K(+)-current was incomplete, the residual K+ current being reduced by 3,4-DAP (0.1 to 5 microM). 4. These results indicate the presence of two aminopyridine-sensitive K+ currents in this preparation distinguished by their susceptibility to DTX.
McGivern
J
J
School of Biomedical Science, Queen's University, Belfast, UK.
Scholfield
C N
CN
Dolly
J O
JO
D015761:4-Aminopyridine / Q000031:analogs & derivatives / Q000494:pharmacology; D000077770:Amifampridine; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism*; D002104:Cadmium / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D004546:Elapid Venoms / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D009186:Myelin Sheath / Q000502:physiology; D009411:Nerve Endings / Q000187:drug effects / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology*; D009830:Olfactory Bulb / Q000187:drug effects / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D004546:Elapid Venoms; D009498:Neurotoxins; D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D002104:Cadmium; C020273:dendrotoxin; D012964:Sodium; D015761:4-Aminopyridine; D000077770:Amifampridine