title: Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE).
abstract: When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable. A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay. The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.
authors.affiliation: Department of Endocrinology, Academic Hospital Utrecht, The Netherlands.
authors.forename: S G
authors.initials: SG
authors.lastname: Koehorst
authors.forename: H M
authors.initials: HM
authors.lastname: Jacobs
authors.forename: J H
authors.initials: JH
authors.lastname: Thijssen
authors.forename: M A
authors.initials: MA
authors.lastname: Blankenstein
mesh_terms: D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001665:Binding Sites; D001943:Breast Neoplasms; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008577:Meningeal Neoplasms / Q000378:metabolism*; D008578:Meninges / Q000378:metabolism; D008579:Meningioma / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis* / Q000378:metabolism; D012016:Reference Values; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D009838:Oligodeoxyribonucleotides; D011960:Receptors, Estrogen