Glutathione and bile acid synthesis. II. Effect of hepatic glutathione content on the activity and mRNA levels of cholesterol 7 alpha-hydroxylase in the rat.
Hepatic cholesterol 7 alpha-hydroxylase (CH-7 alpha) activity in intact rats depleted of glutathione (GSH) was reduced significantly (P < 0.007) compared with that in untreated controls. Northern blot analysis of poly A+ mRNA isolated from GSH-depleted and control rat livers showed that there was a reduction in mRNA for CH-7 alpha in treated rats that was commensurate with the reduction in CH-7 alpha activity. The fact that the level of transferrin mRNA was unaltered by the depletion of GSH suggests that the change in steady-state CH-7 alpha mRNA content is specifically sensitive to GSH content. This observation extends previous in vitro findings and provides strong justification for a more detailed biochemical investigation into the interaction between GSH levels and the regulation of CH-7 alpha mRNA levels.
Hassan
A S
AS
Department of Veterinary Biosciences, University of Illinois, Urbana 61801.
Bunick
D
D
St Denis
S H
SH
Lund
L A
LA
D000818:Animals; D001647:Bile Acids and Salts / Q000096:biosynthesis*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000172:deficiency*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014168:Transferrin / Q000235:genetics
D001647:Bile Acids and Salts; D012333:RNA, Messenger; D014168:Transferrin; D011061:Poly A; D002790:Cholesterol 7-alpha-Hydroxylase; D005978:Glutathione