[Preparation of hybrid proteins consisting of human interleukin-2 and the cytotoxic A-subunit of Shigella toxin].
Recombinant plasmids providing the synthesis of chimeric proteins consisting of amino acid sequences of human interleukin-2 (IL-2) and Shiga toxin cytotoxic A-subunit (ILA and AIL chimeric toxins) were constructed. The ILA and AIL chimeric toxins were shown to inhibit protein synthesis in the rabbit reticulocytes cell-free system. These chimeric toxins displayed two opposite activities of the constituent parts of their molecules on T-lymphocytes from the peripheral blood of healthy volunteers. Hybrid protein AIL (approximately 10(-6) g/ml) has caused the most significant depression of T-lymphoblast proliferation.
Seregin
S V
SV
Siniakov
A N
AN
Pozdniakov
S G
SG
Kozlov
Iu V
IuV
Sakhno
L V
LV
Leplina
O Iu
OIu
Nomokonova
N Iu
NIu
D001427:Bacterial Toxins / Q000737:chemistry*; D001483:Base Sequence; D004274:DNA, Recombinant; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry*; D008969:Molecular Sequence Data; D010957:Plasmids; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D022603:Shiga Toxins
D001427:Bacterial Toxins; D004274:DNA, Recombinant; D007376:Interleukin-2; D011500:Protein Synthesis Inhibitors; D011993:Recombinant Fusion Proteins; D022603:Shiga Toxins
Poluchenie gibridnykh belkov, sostoiashchikh iz interleuĭkina-2 cheloveka i tsitotoksicheskoĭ A-sub''edinitsy toksina shigelly.