Clostridium difficile toxin A elicits Ca(2+)-independent cytotoxic effects in cultured normal rat intestinal crypt cells.
In rat intestinal crypt cells, Clostridium difficile toxin A induces (i) early cytoskeletal alterations involving the whole population and (ii) late effects in 30 to 40% of the cells, consisting mainly of surface blebbing and nuclear fragmentation. All these effects were Ca2+ independent and were not abolished by protein synthesis inhibitors.
Fiorentini
C
C
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
Donelli
G
G
Nicotera
P
P
Thelestam
M
M
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D016360:Clostridioides difficile; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D004768:Enterotoxins / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats
D001427:Bacterial Toxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; D002118:Calcium