Brain banking for immunocytochemistry and autoradiography.
The aim of a human brain bank is to establish groups of matched brains (normal control versus pathological groups) for studying human diseases of the nervous system. This bank is obtained by means of autopsy performed with a very short post-mortem delay and from clinically and neuropathologically well-documented patients. According to research protocols, two types of brain tissue storage are performed: fixed tissue or frozen tissue. Brain dissection procedures are performed according to precise anatomical boundaries of each brain region. This paper will center on the questions raised by brain banking in relation to histological and immunocytochemical studies and to biochemistry and autoradiography of binding sites. The lack of neuroanatomical data of the human brain leads us to compare anatomical results obtained in animals to that of the human. Moreover, it is clear that human brains present numerous interindividual differences (Kopp et al., 1977; Jack et al., 1989). Therefore, investigations of the human brain should be made on a large series of brains indicating the necessity of a well-documented brain bank of tissue from normal controls and patients.
Eymin
C
C
Laboratoire d'Anatomie Pathologique, Faculté de Médecine Alexis Carrel, Lyon, France.
Jordan
D
D
Saint-Pierre
G
G
Kopp
N
N
D001345:Autoradiography; D001665:Binding Sites / Q000502:physiology; D001923:Brain Chemistry; D015925:Cryopreservation; D006652:Histological Techniques; D006801:Humans; D007150:Immunohistochemistry; D013048:Specimen Handling / Q000379:methods; D014015:Tissue Banks / Q000458:organization & administration*