Biochemical activities of denatured covalently closed circular duplex DNA.
Covalently closed circular duplex DNA when exposed to high alkaline pH followed by neutralization yields a collapsed state structure (form I(d)) that can undergo transition to form I and was susceptible to S1 nuclease. Form I(d), in spite of its compact structure, admits specific cleavage by restriction enzymes over its entire genome. When used in a semi-in vitro replication complex, form I(d) gave significantly better template activity, and undergoes better primer extension in in vitro using Klenow. Thus form I(d) having a compact shape can behave as a better substrate in a few key enzymatic reactions.
Santra
C R
CR
Department of Biophysics, Molecular Biology and Genetics, University College of Science, Calcutta, India.
Mukherjee
S K
SK
Thakur
A R
AR
D004261:DNA Replication; D004270:DNA, Circular / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D010944:Plants / Q000235:genetics / Q000378:metabolism
D004270:DNA, Circular