YAC-assisted cloning of transcribed sequences from the human chromosome 3p21 region.
The region surrounding the ZNF35 zinc finger protein gene on 3p21 is of particular interest, as this region of chromosome 3 is frequently involved in rearrangements and/or deletions associated with various human tumors including lung and renal carcinoma. We have analyzed yeast artificial chromosomes (YACs), identified by PCR screening, using oligonucleotides derived from the ZNF35 gene. PFGE and Southern blot/hybridization analysis revealed that the clones cover 750-kb including the ZNF35 gene. The use of specific somatic cell hybrids have allowed us to locate the YAC contig telomeric to the D3F15S2 locus, in a region which is frequently deleted in lung carcinomas. In addition, we have developed a novel cDNA hybridization protocol allowing the isolation of transcribed sequences present in the overlapping YAC clones. Using the cDNA hybridization selection, we have isolated and characterized one transcribed sequence (D3S1362E) from the 3p21 YAC contig and the corresponding cDNA has been isolated. DNA sequencing analysis indicated that the D3S1363E cDNA codes for a putative transcription factor. Northern blot analysis indicated that the D3S1362E sequence hybridized to multiple transcripts in skeletal muscle, and weakly hybridizing transcripts of similar sizes were detected in other tissues.
Pengue
G
G
Dipartimento di Genetica, Biología Generale e Molecolare, University of Napoli, Italy.
Calabrò
V
V
Cannada-Bartoli
P
P
De Luca
P
P
Esposito
T
T
Taillon-Miller
P
P
LaForgia
S
S
Druck
T
T
Huebner
K
K
D'Urso
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016386:Chromosome Walking; D015825:Chromosomes, Fungal; D002893:Chromosomes, Human, Pair 3 / Q000648:ultrastructure*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015723:Gene Library; D016147:Genes, Tumor Suppressor; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics*