In vitro and in vivo assays of isopropanol for mutagenicity.
To assess the mutagenic potential of isopropanol, an in vitro Chinese hamster ovary (CHO) cell/HGPRT gene mutation assay and a bone marrow micronucleus study in mice were conducted. In the CHO/HGPRT assay, concentration levels ranged from 0.5 to 5.0 mg/ml. No elevated mutant frequencies attributable to treatment were observed in the test under either activated or non-activated conditions. In the micronucleus assay, mice were injected intraperitoneally (IP) with either 350, 1,173, or 2,500 mg/kg of isopropanol at constant volumes of 10 ml/kg. No increased incidence of micronuclei was observed in bone marrow polychromatic erythrocytes (PCEs) harvested at 24, 48, or 72 hr post-dosing. In both assays, negative and positive control mutant frequencies were within historical control ranges. These results, in conjunction with previously published data, clearly demonstrate that isopropanol is not a mutagen.
Kapp
R W
RW
Toxicology Division, BioTox, Inc., Richmond, Virginia 23236.
Marino
D J
DJ
Gardiner
T H
TH
Masten
L W
LW
McKee
R H
RH
Tyler
T R
TR
Ivett
J L
JL
Young
R R
RR
D000433:1-Propanol / Q000633:toxicity*; D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001711:Biotransformation; D016466:CHO Cells; D020111:Chlorodiphenyl (54% Chlorine); D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D015162:Micronucleus Tests; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000378:metabolism; D016296:Mutagenesis; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity*; D051381:Rats
D001140:Aroclors; D009153:Mutagens; D020111:Chlorodiphenyl (54% Chlorine); D000433:1-Propanol; D006899:Mixed Function Oxygenases; D007041:Hypoxanthine Phosphoribosyltransferase