title: Identification of a protein product of the myotonic dystrophy gene using peptide specific antibodies.
abstract: In order to identify the protein product of the recently characterised myotonic dystrophy gene, we have raised an antibody (DMAP1) to a peptide sequence of the predicted gene product. This antibody identifies a novel 52 kDa protein in a range of mouse tissues, and in addition a related 42 kDa protein in brain and heart. A second antibody raised to a different peptide from the same predicted sequence, also identifies the 52 kDa protein, which strongly implies that this 52 kDa protein is a major translation product of the myotonic dystrophy gene.
authors.affiliation: Neuromuscular Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
authors.forename: B S
authors.initials: BS
authors.lastname: Brewster
authors.forename: S
authors.initials: S
authors.lastname: Jeal
authors.forename: P N
authors.initials: PN
authors.lastname: Strong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D005799:Genes, Dominant / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry; D009223:Myotonic Dystrophy / Q000235:genetics*; D065636:Myotonin-Protein Kinase; D010446:Peptide Fragments / Q000276:immunology; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification*
chemical_list: C585516:DMPK protein, mouse; D010446:Peptide Fragments; D011506:Proteins; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases