In vitro modification of human immunodeficiency virus type 1 (HIV-1) infectivity by the U937 cells.
The effect of host cell factors on infectivity of human immunodeficiency virus type 1 (HIV-1) was studied by infecting a monoblastoid cell line (U937) or a T-cell line (MOLT-4) with a highly infective single clone of HIV-1 and comparing the infectivity of the produced viruses to different cell lines. Chronically infected U937 cells consistently produced viruses with minimal infectivity. This phenotypic change was host-dependent as the back-passage of the U937-produced low infective viruses into MOLT-4 cells resulted in regaining their original high infectivity. Southern and Northern blot analyses of the HIV-1 grown in U937 cells did not reveal any genomic difference between it and the virus grown it MOLT-4 cells. The radioimmunoprecipitation analysis of viral proteins showed that the HIV-1-infected U937 cells had a different pattern of envelope glycoproteins and core proteins, which well correlated with the low infectivity of the produced viruses. This experimental system using MOLT-4 and U937 cell lines would be useful to further explore host cell factor(s) which play an important role in the regulation of HIV-1 infectivity.
el-Farrash
M A
MA
Department of Biodefence and Medical Virology, Kumamoto University School of Medicine, Japan.
Masuda
T
T
Kuroda
M J
MJ
Harada
S
S
D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002472:Cell Transformation, Viral; D015686:Gene Products, env / Q000378:metabolism; D015750:Genes, gag; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D010641:Phenotype; D011498:Protein Precursors / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D013601:T-Lymphocytes / Q000382:microbiology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; D012367:RNA, Viral