Optimalized in vivo production of monoclonal antibodies in mouse ascitic fluid.
The note contains a brief summary of the method in use at the National Institute of Public Health for the production of monoclonal antibodies in mouse ascitic fluid. This method of obtaining large amounts of monoclonal antibodies is widely used. Refining the in vivo method and increasing the yield from each mouse could result in a reduction of the number of animals used.
Lausund
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P
National Institute of Public Health, Oslo.
Paus
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E
Ingebrigtsen
K
K
D000367:Age Factors; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001202:Ascitic Fluid; D001835:Body Weight; D002273:Carcinogens / Q000008:administration & dosage; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D006825:Hybridomas; D007274:Injections, Intraperitoneal; D008098:Litter Size; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010533:Peritoneal Lavage; D013729:Terpenes / Q000008:administration & dosage
D000911:Antibodies, Monoclonal; D002273:Carcinogens; D013729:Terpenes; C009042:pristane