Molecular cloning and developmental expression of human cardiac troponin T.
We have isolated a full-size cDNA coding for cardiac troponin T (cTnT) from a human adult heart library, using a slow skeletal TnT probe. This cDNA detected a 1.2 kb mRNA in fetal and post-natal human heart, the amount of which increased during ontogenic development. Interestingly, a similar transcript was coexpressed in fetal skeletal muscle, together with the 0.9 kb slow skeletal muscle mRNA, and its expression was down-regulated during further development.
Mesnard
L
L
University of Paris XI, CNRS URA 1159, Hôpital Marie Lannelongue, Le Plessis Robinson, France.
Samson
F
F
Espinasse
I
I
Durand
J
J
Neveux
J Y
JY
Mercadier
J J
JJ
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002648:Child; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D005318:Fetal Heart / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012756:Sheep; D014336:Troponin / Q000235:genetics* / Q000378:metabolism*; D020107:Troponin T
D015342:DNA Probes; D012333:RNA, Messenger; D014336:Troponin; D020107:Troponin T; D004247:DNA