Proteolytic processing of the vitellogenin precursor in the boll weevil, Anthonomus grandis.
The soluble proteins of the eggs of the coleopteran insect Anthonomus grandis Boheman, the cotton boll weevil, consist almost entirely of two vitellin types with M(r)s of 160,000 and 47,000. We sequenced their N-terminal ends and one internal cyanogen bromide fragment of the large vitellin and compared these sequences with the deduced amino acid sequence from the vitellogenin gene. The results suggest that both the boll weevil vitellin proteins are products of the proteolytic cleavage of a single precursor protein. The smaller 47,000 M(r) vitellin protein is derived from the N-terminal portion of the precursor adjacent to an 18 amino acid signal peptide. The cleavage site between the large and small vitellins at amino acid 362 is adjacent to a pentapeptide sequence containing two pairs of arginine residues. Comparison of the boll weevil sequences with limited known sequences from the single 180,000 M(r) honey bee protein show that the honey bee vitellin N-terminal exhibits sequence homology to the N-terminal of the 47,000 M(r) boll weevil vitellin. Treatment of the vitellins with an N-glycosidase results in a decrease in molecular weight of both proteins, from 47,000 to 39,000 and from 160,000 to 145,000, indicating that about 10-15% of the molecular weight of each vitellin consists of N-linked carbohydrate. The molecular weight of the deglycosylated large vitellin is smaller than that predicted from the gene sequence, indicating possible further proteolytic processing at the C-terminal of that protein.
Heilmann
L J
LJ
Biosciences Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Fargo, ND 58105.
Trewitt
P M
PM
Kumaran
A K
AK
D000595:Amino Acid Sequence; D000818:Animals; D001517:Coleoptera / Q000235:genetics / Q000378:metabolism*; D004527:Egg Proteins / Q000096:biosynthesis* / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics; D006031:Glycosylation; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid; D014819:Vitellogenins / Q000096:biosynthesis* / Q000235:genetics
D004527:Egg Proteins; D011498:Protein Precursors; D014819:Vitellogenins