title: The petunia homologue of the Antirrhinum majus candi and Zea mays A2 flavonoid genes; homology to flavanone 3-hydroxylase and ethylene-forming enzyme.
abstract: The synthesis of anthocyanins in higher plants involves many enzymatic steps. Here we describe the isolation and characterization of a cDNA, ant17, which encodes a protein that has 73% amino acid sequence identity with the candi gene product of Antirrhinum majus and 48% with that of the maize a2 gene. This protein may therefore be involved in the synthesis of anthocyanins in the steps after the action of dihydroflavonol 4-reductase. This is consistent with the absence of ant17 expression in the regulatory anthocyanin mutants of petunia an1, an2 and an11. Furthermore, ant17 is predominantly expressed in corollas and anthers and is induced by gibberellic acid.
authors.affiliation: Department of Genetics, Vrije Universiteit, Amsterdam, Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Weiss
authors.forename: A H
authors.initials: AH
authors.lastname: van der Luit
authors.forename: J T
authors.initials: JT
authors.lastname: Kroon
authors.forename: J N
authors.initials: JN
authors.lastname: Mol
authors.forename: J M
authors.initials: JM
authors.lastname: Kooter
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification; D005419:Flavonoids / Q000235:genetics*; D008190:Lyases / Q000235:genetics*; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants; D017386:Sequence Homology, Amino Acid; D003313:Zea mays
chemical_list: C082801:Ant17 protein, Petunia; D005419:Flavonoids; D010940:Plant Proteins; D004247:DNA; D006899:Mixed Function Oxygenases; C048797:flavanone 3-dioxygenase; D008190:Lyases; C034693:ethylene forming enzyme