title: Comparative studies of intracellular activity, secretion and multiple forms spectra of human skin fibroblast alpha-L-fucosidase in the normaland after sucrose load.
abstract: Activity and multiple forms of intracellular and secreted alpha-L-fucosidases from human skin fibroblasts were studied in normal and sucrose-loaded cells--modelling intralysosomal nonhydrolyzable products' accumulation in vitro. Certain differences in secretion levels were found after sucrose load for alpha-L-fucosidases from embryonal and postnatal cell lines. Different effects of sucrose load on multiple forms' spectra of the intracellular and secreted alpha-L-fucosidases from embryonal and postnatal fibroblasts were revealed.
authors.affiliation: Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow.
authors.forename: E M
authors.initials: EM
authors.lastname: Beyer
authors.forename: T S
authors.initials: TS
authors.lastname: Ivleva
authors.forename: G T
authors.initials: GT
authors.lastname: Artykova
authors.lastname: Wiederschain GYa
mesh_terms: D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology; D012867:Skin / Q000201:enzymology*; D013395:Sucrose / Q000494:pharmacology*; D005644:alpha-L-Fucosidase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D013395:Sucrose; D005644:alpha-L-Fucosidase