title: The optimisation of a novel iodide microassay and its application in an immunoassay for human antibody levels in serum.
abstract: Radioactive iodine has been used as an antibody label in many immunoassays. The feasibility of using non-radioactive iodine as a label was investigated. A microassay for iodide based on the Sandell-Kolthoff reaction was optimised. It was used to detect iodine-labelled antibody and successfully applied to the detection of human IgG in serum samples. The performance of this assay system compared with enzyme-linked immunosorbent assay using HRP was evaluated.
authors.affiliation: School of Biological Sciences, Dublin City University, Ireland.
authors.forename: R
authors.initials: R
authors.lastname: O'Kennedy
authors.forename: P
authors.initials: P
authors.lastname: Keating
mesh_terms: D000888:Antibodies, Anti-Idiotypic; D002563:Cerium; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens; D006735:Horseradish Peroxidase; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood*; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D012680:Sensitivity and Specificity; D013388:Succinimides; D013431:Sulfates
chemical_list: D000888:Antibodies, Anti-Idiotypic; D006241:Haptens; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D013388:Succinimides; D013431:Sulfates; D002563:Cerium; C022581:Bolton-Hunter reagent; C082771:ceric ammonium sulfate; D006735:Horseradish Peroxidase