Use of an automated laboratory workstation for isolation of genomic DNA suitable for PCR and allele-specific hybridization.
In this paper we describe the isolation of genomic DNA by using anion exchange chromatography performed on a Biomek 1000 Automated Laboratory Workstation. This procedure allows the automated isolation of DNA suitable for most molecular analyses employed in diagnosis of genetic pathologies and infectious diseases. The genomic DNA isolated by using the Biomek 1000 was indeed found to be suitable for polymerase chain reaction and allele-specific hybridization.
Mischiati
C
C
Istituto di Chimica Biologica, Ferrara, Italy.
Fiorentino
D
D
Feriotto
G
G
Gambari
R
R
D000483:Alleles; D000818:Animals; D001322:Autoanalysis; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D004247:DNA / Q000302:isolation & purification*; D011905:Genes, ras; D006801:Humans; D007938:Leukemia; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D004247:DNA