cDNA cloning of the sodium-influx-stimulating peptide in the mollusc, Lymnaea stagnalis.
We isolated and characterized a cDNA clone, encoding the prohormone of the sodium-influx-stimulating (SIS) peptide of the freshwater snail Lymnaea stagnalis. The prohormone is cleaved to generate a signal peptide of 23 amino acids and a SIS peptide of 77 amino acids. The SIS peptide as encoded by the cDNA represents a novel and complex neuropeptide, which controls the activity of sodium pumps in the integument, pericardium, ureter and nephridial gland. In situ hybridization showed that the SIS-peptide gene is expressed by the neuroendocrine, so-called Yellow Cells of the central nervous system.
Smit
A B
AB
Graduate School Neurosciences Amsterdam, Faculty of Biology, Department of Zoology, Vrije Universiteit, The Netherlands.
Thijsen
S F
SF
Geraerts
W P
WP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005724:Ganglia / Q000737:chemistry; D015870:Gene Expression; D017403:In Situ Hybridization; D008195:Lymnaea / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics; D021382:Protein Sorting Signals / Q000737:chemistry; D012964:Sodium / Q000378:metabolism*
D009479:Neuropeptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; C082469:sodium-influx-stimulating peptide; D004247:DNA; D012964:Sodium