Chromosome mapping in Alcaligenes eutrophus CH34.
Mutants and mobilizing plasmids were developed as genetic tools in Alcaligenes eutrophus CH34. In order to map the chromosome, spontaneous and ethyl methane sulphonate (EMS)-induced mutants (mostly auxotrophs) were isolated. Another source of mutants was provided by the phenomenon of temperature-induced mortality and mutagenesis that is observed at 37 degrees C and is characteristic of many metallotolerant strains of A. eutrophus. Plasmid pULB113 (RP4::miniMu) was used to map the available mutations. Twenty-five loci were ordered in a circular map. pMOL50, a rearranged derivative of plasmid pMOL28, which was obtained in a survivor at 37 degrees C and displayed chromosome mobilizing activity (Cma+), was also used to mobilize chromosomal markers: resulting linkages were stronger than with pULB113, allowing confirmation of the circularity of the A. eutrophus CH34 chromosome with a small number of crosses.
Sadouk
A
A
Laboratoire de Génétique et Biotechnologie, Centre d'Etudes de l'Energie Nucléaire and Vlaamse Instelling voor Technologisch Onderzoek, SCK/CEN-VITO, Mol, Belgium.
Mergeay
M
M
D000421:Alcaligenes / Q000187:drug effects / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D008040:Genetic Linkage; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D010957:Plasmids; D013696:Temperature
D008769:Methylnitronitrosoguanidine; D005020:Ethyl Methanesulfonate