Effect of Berenil on polyamine metabolism in primary cultured rat hepatocytes.
1. Putrescine and spermidine content increased in hepatocytes during culture. In the presence of 10 microM Berenil, putrescine content was further increased, while the increase of spermidine was prevented. 2. Ornithine decarboxylase activity was markedly reduced, and to a lesser extent also S-adenosylmethionine decarboxylase activity. 3. Berenil appears to promote an increase in the transformation of spermidine into putrescine, and to inhibit the polyamine efflux.
Colombatto
S
S
Dipartimento di Medicina e Oncologia sperimentale, Università di Torino, Italy.
Grillo
M A
MA
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004133:Diminazene / Q000031:analogs & derivatives* / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism*; D051381:Rats; D013095:Spermidine / Q000378:metabolism*
D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; C003915:diminazene aceturate; D013095:Spermidine; D011700:Putrescine; D004133:Diminazene