Preparation of fluorescence labeled insulins, sulfobenzoxadiazolyl-insulins, for fluorescence immunoassay.
The preparation of insulins labeled with a fluorescent moiety at a definite position (Gly(A1) or Lys(B29)) on the molecule is described. Gly(A1)- and Lys(B29)-S-acetylthioglycoloyl-insulins and Gly(A1)-2(or 3)-acetylmercapto-3-carboxypropranoyl-insulin were deacetylated and then reacted with 7-chloro-4-sulfobenzoxadiazole, a fluorogenic reagent, to afford the corresponding fluorescence labeled insulins. The preparative separation of the fluorescence labeled insulins from the insulin derivatives that remained unreacted was carried out by anion-exchange high-performance liquid chromatography on a TSKgel DEAE-2SW column.
Nanami
M
M
Faculty of Pharmaceutical Sciences, Kyushu University 62, Fukuoka, Japan.
Zaitsu
K
K
Ohkura
Y
Y
D002851:Chromatography, High Pressure Liquid; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007328:Insulin / Q000737:chemistry* / Q000276:immunology; D010069:Oxadiazoles; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007328:Insulin; D010069:Oxadiazoles; C083020:7-chloro-4-sulfobenzoxadiazole