Neosurugatoxin blocks an alpha-bungarotoxin-sensitive neuronal nicotinic acetylcholine receptor.
Neosurugatoxin (NSTX), a neurotoxin isolated from the Japanese ivory mollusc Babylonia japonica, is a potent neuronal nicotinic acetylcholine receptor (nAChR) antagonist. Hitherto, NSTX has been found to block only neuronal nAChRs that are insensitive to alpha-Bgt. Here, we report for the first time that NSTX blocks an alpha-Bgt-sensitive nAChR on an identified insect motor neurone. Bath-applied NSTX at a concentration of 10 nM and above reversibly blocks the nicotine-induced depolarizations recorded from the cockroach (Periplaneta americana) fast coxal depressor motor neurone (Df) and is without effect on GABA-induced responses detected on the same cell. NSTX is among the most potent blockers tested to date on nAChRs of motor neurone Df.
Bai
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Department of Zoology, University of Cambridge, United Kingdom.
Sattelle
D B
DB
D000818:Animals; D003058:Cockroaches; D005724:Ganglia; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008978:Mollusk Venoms / Q000633:toxicity*; D009046:Motor Neurons / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D009498:Neurotoxins / Q000633:toxicity*; D009538:Nicotine / Q000494:pharmacology; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000378:metabolism; D012717:Sesquiterpenes / Q000633:toxicity; D012908:Snails; D012910:Snake Venoms / Q000494:pharmacology; D014714:Vertebrates; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D008978:Mollusk Venoms; D009498:Neurotoxins; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic; D012717:Sesquiterpenes; D012910:Snake Venoms; D005680:gamma-Aminobutyric Acid; D009538:Nicotine; C036793:neosurugatoxin; C069649:bipinnatin B