title: Antipromoting effects of 5-lipoxygenase inhibitors, 3-nitro-2,4,6- trihydroxybenzamide derivatives, on TPA-promoted transformation in BALB 3T3 cells.
abstract: In order to discover the role in the promotion process of 5-lipoxygenase (5-LO), a key enzyme in the arachidonate cascade, the antipromoting effects of the 5-LO inhibiting 3-nitro-2,4,6-trihydroxybenzamide (NTB) derivatives were studied in a two-stage transformation assay system using BALB 3T3 cells. All compounds inhibited TPA-promoted transformation in a dose-dependent manner. Most of them achieved a 70-80% inhibition. Good correlations were observed between the inhibition of TPA-promoted transformation and that of 5-LO. These results indicate that 5-LO plays an important role in the promotion stage of the transformation of BALB 3T3 cells.
authors.affiliation: Quality Assurance Unit, Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Semba
authors.forename: I
authors.initials: I
authors.lastname: Honda
authors.forename: M
authors.initials: M
authors.lastname: Shibagaki
authors.forename: Y
authors.initials: Y
authors.lastname: Nishi
mesh_terms: D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D002471:Cell Transformation, Neoplastic; D004305:Dose-Response Relationship, Drug; D006877:Hydroxamic Acids / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors; D008748:Methylcholanthrene; D051379:Mice; D013755:Tetradecanoylphorbol Acetate
chemical_list: D006877:Hydroxamic Acids; D016859:Lipoxygenase Inhibitors; D008748:Methylcholanthrene; D013755:Tetradecanoylphorbol Acetate