Effect of spacer groups connecting insulin with fluorophore in Gly(A1)-sulfobenzoxadiazole-labeled insulins on the immunoreactivity toward anti-insulin antibody.
The immunoreactivity (specific binding) of three Gly(A1)-sulfobenzoxadiazole-labeled insulins which have different spacer groups, Gly(A1)-[3-(4-sulfobenzoxadiazol-7-ylthio)propanoyl]-insulin , Gly(A1)-[2-(4-sulfobenzoxadiazol-7-ylthio)acetyl]-insulin, Gly(A1)-[3-carboxy-2(or 3)-(4-sulfobenzoxadiazol-7-ylthio)propanoyl]-insulin, toward an antiporcine insulin monoclonal antibody was investigated, where solid-phase fluoroimmunoassay technique was utilized. The immunoreactivities of three labeled insulins were 2.1, 1.8 and 1 in that order.
Nanami
M
M
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Zaitsu
K
K
Ohkura
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007328:Insulin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D007330:Insulin Antibodies / Q000276:immunology*; D013552:Swine
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007328:Insulin; D007330:Insulin Antibodies