title: Cloning and characterization of Neisseria meningitidis genes encoding the transferrin-binding proteins Tbp1 and Tbp2.
abstract: Genes tbp1 and tbp2, encoding the transferrin-binding proteins Tbp1 and Tbp2, have been isolated from two strains of Neisseria meningitidis. The tbp2 and tbp1 open reading frames are tandemly arranged in the genome with an 87-bp intergenic region, and the DNA region upstream from the tbp2-coding sequence contains domains homologous to Escherichia coli promoter consensus motives. Nucleotide sequence analysis suggests the existence of a Tbp1 precursor carrying an N-terminal signal peptide with a peptidase I cleavage site and of a Tbp2 precursor with N-terminal homology to lipoproteins, including a peptidase II cleavage site. Comparison of the Tbp1 deduced amino acid (aa) sequences from both strains showed about 76% aa homology, while those of Tbp2 revealed only about 47% aa homology. These comparisons should be extended to other Neisseria strains in order to evaluate further this genetic divergence further.
authors.affiliation: Transgène, Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Legrain
authors.forename: V
authors.initials: V
authors.lastname: Mazarin
authors.forename: S W
authors.initials: SW
authors.lastname: Irwin
authors.forename: B
authors.initials: B
authors.lastname: Bouchon
authors.forename: M J
authors.initials: MJ
authors.lastname: Quentin-Millet
authors.forename: E
authors.initials: E
authors.lastname: Jacobs
authors.forename: A B
authors.initials: AB
authors.lastname: Schryvers
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics; D033862:Iron-Binding Proteins; D008074:Lipoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000235:genetics*; D016366:Open Reading Frames; D021382:Protein Sorting Signals / Q000235:genetics; D011990:Receptors, Transferrin / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014168:Transferrin / Q000378:metabolism; D033863:Transferrin-Binding Proteins
chemical_list: D000942:Antigens, Bacterial; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D008074:Lipoproteins; D021382:Protein Sorting Signals; D011990:Receptors, Transferrin; D014168:Transferrin; D033863:Transferrin-Binding Proteins