title: The effect of acute nonbacterial dependent peritonitis on lung and liver oxidant stress and antioxidant activity.
abstract: BACKGROUND
  The role of oxidant release and tissue antioxidant defenses on inflammation-induced organ injury is not clearly defined.
  
  
  METHODS
  We determined the effect of acute zymosan peritonitis in rats on lung and liver tissue oxidant stress and antioxidant defenses during a 5-day period. Oxidant activity was measured as tissue malondialdehyde and oxidized glutathione (GSSG). Antioxidant activity was measured as tissue-reduced glutathione (GSH) and catalase activity. Rats were maintained hydrated with subcutaneous crystalloid. Animals were killed at 4, 12, and 24 hours and 5 days.
  
  
  RESULTS
  Acute peritonitis was evident at 12 and 24 hours but was resolving at 5 days. Peritoneal fluid cultures were negative after 24 hours. A 50% mortality rate was noted between 20 and 30 hours, with no deaths after 30 hours. We noted a significant increase in lung GSSG and malondialdehyde at 4 hours that persisted for the 5 days, as did histologic evidence of a progressing severe lung inflammation. No increased conversion of lung xanthine dehydrogenase to xanthine oxidase was noted. Lung GSH and catalase activity were maintained at control despite negligible food intake. In contrast, liver GSSG was increased significantly only at the 4-hour period, corresponding with a transient conversion of xanthine dehydrogenase to xanthine oxidase from 10% to 31%. Tissue malondialdehyde did not increase despite the initial oxidant stress. However, tissue GSH and catalase values decreased by more than 50% after 24 hours and remained decreased at 5 days.
  
  
  CONCLUSIONS
  We conclude that early lung and liver oxidant stress is initiated by acute peritonitis. Lung oxidant changes persist and lung dysfunction progresses, even though antioxidant activity is maintained and acute peritonitis is resolving. Liver lipid peroxidation did not develop despite oxidant release, probably because of a large antioxidant reserve. However, a severe and sustained decrease in liver antioxidants results, increasing the potential damage from a subsequent oxidant insult.
authors.affiliation: Longwood Area Trauma Center, Brigham and Women's Hospital, Boston, Mass.
authors.forename: R
authors.initials: R
authors.lastname: Demling
authors.forename: R
authors.initials: R
authors.lastname: Daryani
authors.forename: C
authors.initials: C
authors.lastname: Campbell
authors.forename: J
authors.initials: J
authors.lastname: Knox
authors.forename: Y K
authors.initials: YK
authors.lastname: Youn
authors.forename: C
authors.initials: C
authors.lastname: Lalonde
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001835:Body Weight; D002374:Catalase / Q000378:metabolism; D004231:Diuresis; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D019803:Glutathione Disulfide; D006400:Hematocrit; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D016877:Oxidants / Q000378:metabolism*; D010100:Oxygen / Q000097:blood; D010538:Peritonitis / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014968:Xanthine Dehydrogenase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism; D015054:Zymosan
chemical_list: D000975:Antioxidants; D016877:Oxidants; D015054:Zymosan; D002374:Catalase; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase; D005978:Glutathione; D010100:Oxygen; D019803:Glutathione Disulfide