Human peripheral blood xenografts in the SCID mouse: characterization of immunologic reconstitution.
Immune reconstitutions (hu-PBL-SCID mice) resulting from adoptive transfer of human peripheral blood mononuclear cells into 1800 C.B-17 scid-/scid-mice were characterized. Over 90% of reconstitutions were successful as evidenced by human immunoglobulin production. Variability was noted with donor, cell number, and cell type. Human cells (T lymphocytes, few B cells) could be recovered by 5 days after engraftment. High levels of soluble CD8 and interleukin-2 receptors were detected in sera of hu-PBL-SCID mice. Cells recovered from 17 mice proliferated in response to antigens to which the donor had been primed; responses to nonboosted antigen also increased in some animals. After reconstitution, lymphocytes were found in the spleen and lymph nodes without full restoration of normal architecture. The hu-PBL-SCID mouse shows promise as a model system for a variety of immunologic studies. The inherent variation in the system must be minimized for appropriate use of the model.
Hesselton
R M
RM
University of Massachusetts Medical School, Dept. of Pediatrics, Worcester 01605.
Koup
R A
RA
Cromwell
M A
MA
Graham
B S
BS
Johns
M
M
Sullivan
J L
JL
D000367:Age Factors; D000818:Animals; D001773:Blood Cells / Q000166:cytology; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D006801:Humans; D007107:Immune System / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D051379:Mice; D016513:Mice, SCID / Q000276:immunology*; D008954:Models, Biological; D010529:Peritoneal Cavity / Q000166:cytology; D010641:Phenotype; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D014183:Transplantation, Heterologous
D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D007136:Immunoglobulins; D015375:Receptors, Interleukin-2