title: In vivo infection of human T-cell leukemia virus type I in non-T cells.
abstract: Using three different procedures, immunomagnetic isolation, FACS and panning, each of the T4 cell, T8 cell, monocyte, and B cell population was fractionated from peripheral blood mononuclear cells (PBMC) obtained from 10 human T cell leukemia virus type I (HTLV-I)-associated myelopathy (HAM)/tropical spastic paraparesis (TSP) cases, 3 adult T cell leukemia and lymphoma (ATLL) cases and 9 HTLV-I-infected healthy carriers. DNA from each fraction was then subjected to the quantitative polymerase chain reaction. Although T4 cells possessed the highest copy number of HTLV-I in general, we also detected a significant amount of HTLV-I provirus in T8 cells, monocytes, and B cells. To confirm virus infection of the non-T cell populations, especially the monocyte fraction, we performed further experiments and the following results were obtained: (1) HTLV-I tax RNA expression was confirmed simultaneously in the RNA of monocytes that were negative for T cell receptor beta chain (TCR beta) expression in vivo. (2) We cultured these monocytes for a short period of time and detected HTLV-I antigen expression in non-specific esterase-positive monocyte/macrophage cells. Our data indicate that HTLV-I has a broad host range in vivo and that monocytes or cells of monocyte lineage such as tissue macrophages might comprise a virus reservoir in vivo.
authors.affiliation: Department of Microbiology, Tokyo Medical and Dental University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Koyanagi
authors.forename: Y
authors.initials: Y
authors.lastname: Itoyama
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Takamatsu
authors.forename: J
authors.initials: J
authors.lastname: Kira
authors.forename: T
authors.initials: T
authors.lastname: Iwamasa
authors.forename: I
authors.initials: I
authors.lastname: Goto
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000382:microbiology*; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral; D015486:HTLV-I Antigens / Q000096:biosynthesis; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000382:microbiology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000382:microbiology*; D015493:Paraparesis, Tropical Spastic / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000382:microbiology*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D015486:HTLV-I Antigens