(+)Naloxone potentiates the inotropic effect of epinephrine in the isolated dog heart.
Naloxone potentiates the inotropic effect of circulating catecholamines in the isolated canine heart. The stereospecificity of this response was evaluated with the aid of the less active (+)enantiomer of naloxone. The more common (-)isomer of naloxone increased the contractile response to epinephrine only at the higher dose tested (4 mg). This effect of naloxone was not observed at a tenfold lower dose (0.4 mg), indicating a very narrow dose-response range. (+)Naloxone was effective at the lower dose and was, therefore, equal to or better than (-)naloxone in potentiating the inotropic effect of epinephrine. When introduced afterward, (-)naloxone did not add to the effect of (+)naloxone. These data suggest that naloxone modifies cellular responsiveness to catecholamines through a nontraditional opiate receptor, through a nonopiate receptor, or through a nonreceptor mechanism.
Gu
H
H
Department of Physiology, Texas College of Osteopathic Medicine, Fort Worth 76107.
Barron
B A
BA
Gaugl
J F
JF
Caffrey
J L
JL
D000818:Animals; D004285:Dogs; D004357:Drug Synergism; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D009270:Naloxone / Q000494:pharmacology*; D013237:Stereoisomerism; D013268:Stimulation, Chemical
D009270:Naloxone; D004837:Epinephrine