title: Isoproterenol stimulates rapid extrusion of sodium from isolated smooth muscle cells.
abstract: beta-Agonists cause an inhibition of contractility and a transient stimulation of Na+/K+ pumping in smooth muscle cells of the stomach from the toad Bufo marinus. To determine if the stimulation of Na+/K+ pumping causes changes in intracellular [Na+] ([Na+]i) that might link Na+ pump stimulation to decrease Ca2+ availability for contraction, [Na+]i was measured in these cells with SBFI, a Na(+)-sensitive fluorescent indicator. Basal [Na+]i was 12.8 +/- 4.2 mM (n = 32) and was uniform throughout the cell. In response to isoproterenol, [Na+]i decreased an average of 7.1 +/- 1.1 mM in 3 sec. Since this decrease in [Na+]i could be completely blocked by inhibition of the Na+ pump, or by blockade of the beta-receptor, [Na+]i reduction is the result of occupation of the beta-receptor by isoproterenol and subsequent stimulation of the Na+ pump. 8-Bromoadenosine 3',5'-cyclic monophosphate and forskolin mimicked the effect of isoproterenol, indicating that the sequence of events linking beta-receptor occupation to Na+ pump stimulation most likely includes activation of adenylate cyclase, production of cAMP, and stimulation of cAMP-dependent protein kinase. The decrease in [Na+]i is sufficiently large and fast that it is expected to stimulate turnover of the Na+/Ca2+ exchanger in the Ca2+ extrusion mode, thereby accounting for the observed linkage between stimulation of the Na+/K+ pump and inhibition of contractility in response to beta-adrenergic agonists.
authors.affiliation: Department of Physiology, University of Massachusetts Medical Center, Worcester 01605.
authors.forename: E D
authors.initials: ED
authors.lastname: Moore
authors.forename: F S
authors.initials: FS
authors.lastname: Fay
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D001572:Benzofurans; D002024:Bufo marinus; D002217:Carbachol / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D004988:Ethers, Cyclic; D005456:Fluorescent Dyes; D005753:Gastric Mucosa / Q000378:metabolism; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology*; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D010869:Pindolol / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D013270:Stomach / Q000166:cytology / Q000187:drug effects; D013997:Time Factors
chemical_list: D001572:Benzofurans; D004988:Ethers, Cyclic; D005456:Fluorescent Dyes; C061665:sodium-binding benzofuran isophthalate; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D010042:Ouabain; D002217:Carbachol; D012964:Sodium; D010869:Pindolol; D007545:Isoproterenol; D011188:Potassium