Chloramine-T induced binding of monoclonal antibody B72.3 to concanavalin-A.
The effects of chloramine-T (CT) on monoclonal antibody B72.3 were studied with particular reference to Con-A lectin binding. After exposure to chloramine-T concentrations from 0.8 to 4.0 mg/mL (115-574 mol CT/mol B72.3), B.72.3 showed progressive binding to agarose-linked Con-A. This behavior was paralleled by decreasing immunoreactivity and increasing fragmentation and aggregation of B72.3 demonstrated by SDS-PAGE and size exclusion HPLC.
Cole
W C
WC
Methodist Hospital/Baylor College of Medicine, Houston, TX 77030.
Jhingran
S G
SG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001666:Binding Sites, Antibody / Q000187:drug effects; D002700:Chloramines / Q000494:pharmacology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D051379:Mice; D014105:Tosyl Compounds
D000911:Antibodies, Monoclonal; D002700:Chloramines; D014105:Tosyl Compounds; C016300:chloramine-T; D003208:Concanavalin A