Evaluation of indoxyl-beta-D-glucuronide as a chromogen in media specific for Escherichia coli.
Indoxyl-beta-D-glucuronide (indoxyl) was evaluated as a specific chromogen for detection of Escherichia coli by the membrane filter method. In all, 413 colonies were tested from the indoxyl-supplemented media, yielding 93.3% confirmation, as E. coli. Compared with the indoxyl medium, other media gave either much lower recovery with high verification or equal recovery with poor verification.
Haines
J R
JR
Risk Reduction Engineering Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
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D001431:Bacteriological Techniques; D004396:Coloring Agents; D003470:Culture Media; D004926:Escherichia coli / Q000302:isolation & purification / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005965:Glucuronates / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D014871:Water Microbiology
D004396:Coloring Agents; D003470:Culture Media; D005965:Glucuronates; D007211:Indoles; C059836:indoxyl glucuronide