Induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1.
Osteogenic protein-1 (OP-1, BMP-7), a member of the transforming growth factor-beta supergene family, induces cartilage and bone formation when implanted in intra- and extraskeletal sites in vivo. The human OP-1 gene has been cloned and biologically active recombinant OP-1 homodimers (hOP-1) produced. The amount of bone induced by hOP-1 in vivo is related to the amount of protein implanted. Dentine possesses bone morphogenetic protein (BMP) activity. Impure material from allogenic bone with BMP activity induced reparative dentine formation in dogs. The objective of this study was to determine if the amount of reparative dentine stimulated by hOP-1 is related to the amount of protein utilized in direct pulp-capping experiments. Freshly exposed molar and premolar pulps were treated with varying amounts of a complex comprising hOP-1 and a carrier matrix of purified bovine type-1 collagen powder (CM) moistened with sterile saline. Reparative dentine was present in all hOP-1/CM treated teeth (12 of 15) that remained sealed for the 6 weeks' healing. Substantially more new dentine was present in teeth treated with hOP-1/CM than in those treated with Ca(OH)2 paste and the amount of reparative dentine formed was proportional to the amount of hOP-1/CM (P < 0.05). No reparative dentine formed in collagen carrier or untreated teeth. The appearances of the new tissue suggested that much of the mass of the hOP-1/CM was replaced first by a pulp-like connective tissue, which mineralized to form reparative dentine.(ABSTRACT TRUNCATED AT 250 WORDS)
Rutherford
R B
RB
University of Connecticut School of Dental Medicine, Farmington 06032.
Wahle
J
J
Tucker
M
M
Rueger
D
D
Charette
M
M
D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D002126:Calcium Hydroxide / Q000627:therapeutic use; D003094:Collagen; D003782:Dental Pulp / Q000033:anatomy & histology / Q000187:drug effects; D003785:Dental Pulp Capping; D003809:Dentin, Secondary / Q000033:anatomy & histology / Q000139:chemically induced* / Q000502:physiology; D005260:Female; D006801:Humans; D008252:Macaca fascicularis; D014677:Pharmaceutical Vehicles; D011506:Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000627:therapeutic use*; D014945:Wound Healing
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D014677:Pharmaceutical Vehicles; D011506:Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D003094:Collagen; D002126:Calcium Hydroxide