Characterisation of 2S albumin with nutritionally balanced aminoacid composition from the seeds of Chenopodium album and its antigenic homology with seed proteins of some Chenopodiaceae and Amaranthaceae species.
The low molecular weight 2S albumin protein of Chenopodium album seeds has been isolated and characterized with respect to its subunit structure by SDS-PAGE and antigenic homology with low molecular weight seed storage proteins of several other phylogenetically related species by immunoprecipitation and Western blotting. These studies revealed the existence of antigenically homologous proteins of similar molecular weights in seeds of some other members of Chenopodiaceae and Amaranthaceae. However, Chenopodium 2S albumin is antigenically unrelated to low mol.wt. albumins of dicots belonging to other families. It has a nutritionally balanced aminoacid composition in respect of essential aminoacids.
Dey
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Department of Biochemistry, Bose Institute, Calcutta, India.
Mandal
R K
RK
D000418:Albumins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D000596:Amino Acids / Q000032:analysis*; D000941:Antigens / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D010944:Plants / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
D000418:Albumins; D000596:Amino Acids; D000941:Antigens; D010940:Plant Proteins