title: [A new method of isolation and purification of SsoII restriction endonuclease].
abstract: A new method for isolation and purification of restriction endonuclease SsoII which results in a homogeneous preparation suitable for all types of fine physico-chemical assays has been elaborated. The procedure includes four chromatographic steps: fractionation on butyl-Toyopearl, combined chromatography on SP-Toyopearl and phosphocellulose PII, and chromatography on DEAE-Toyopearl and on QAE-Toyopearl. The use of fast flow sorbents (Toyopearl) makes it possible to reduce the time needed for the separation of proteins and to optimize the fractionation conditions, thus avoiding the dialysis between the chromatographic steps which significantly decreased the enzyme activity yields in previous purification schemes. The isolation of restriction endonuclease SsoII by the new method usually takes four days.
authors.forename: A S
authors.initials: AS
authors.lastname: Kariagina
authors.forename: I Ia
authors.initials: IIa
authors.lastname: Levchenko
authors.forename: I I
authors.initials: II
authors.lastname: Nikol'skaia
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology
chemical_list: C045297:CCNGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Novyĕ sposob vydeleniia i ochistki éndonukleazy restriktsii SsoII.