title: Insulin-like growth factor I activates the invasion suppressor function of E-cadherin in MCF-7 human mammary carcinoma cells in vitro.
abstract: The calcium-dependent cell-cell adhesion molecule E-cadherin has been shown to counteract invasion of epithelial neoplastic cells. Using three monoclonal antibodies, we have demonstrated the presence of E-cadherin at the surface of human MCF-7/6 mammary carcinoma cells by indirect immunofluorescence coupled to flow cytometry and by immunocytochemistry. Nevertheless, MCF-7/6 cells failed to aggregate in a medium containing 1.25 mM CaCl2, and they were invasive after confrontation with embryonic chick heart fragments in organ culture. Treatment of MCF-7/6 cells with 0.5 microgram ml-1 insulin-like growth factor I (IGF-I) led to homotypic aggregation within 5 to 10 min and inhibited invasion in vitro during at least 8 days. The effect of IGF-I on cellular aggregation was insensitive to cycloheximide. However, monoclonal antibodies that interfered with the function of either the IGF-I receptor (alpha IR3) or E-cadherin (HECD-1, MB2) blocked the effect of IGF-I on aggregation. The effects of IGF-I on aggregation and on invasion could be mimicked by 1 microgram ml-1 insulin, but not by 0.5 microgram ml-1 IGF-II. The insulin effects were presumably not mediated by the IGF-I receptor, since they could not be blocked by an antibody against this receptor (alpha IR3). Our results indicate that IGF-I activates the invasion suppressor role of E-cadherin in MCF-7/6 cells.
authors.affiliation: Department of Radiotherapy and Nuclear Medicine, University Hospital, Gent, Belgium.
authors.forename: M E
authors.initials: ME
authors.lastname: Bracke
authors.forename: B M
authors.initials: BM
authors.lastname: Vyncke
authors.forename: E A
authors.initials: EA
authors.lastname: Bruyneel
authors.forename: S J
authors.initials: SJ
authors.lastname: Vermeulen
authors.forename: G K
authors.initials: GK
authors.lastname: De Bruyne
authors.forename: N A
authors.initials: NA
authors.lastname: Van Larebeke
authors.forename: K
authors.initials: K
authors.lastname: Vleminckx
authors.forename: F M
authors.initials: FM
authors.lastname: Van Roy
authors.forename: M M
authors.initials: MM
authors.lastname: Mareel
mesh_terms: D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000473:pathology; D015820:Cadherins / Q000032:analysis / Q000378:metabolism*; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D007700:Kinetics; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015820:Cadherins; D007328:Insulin; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II