Immunocytochemical localization of 5-HT1A receptors in the rat immature cerebellum.
The serotonin 5-HT1A receptors were visualized in the cerebellar vermis of 8-day-old and adult rats by immunocytochemistry using anti-5-HT1A receptor antibodies raised against a synthetic peptide corresponding to a highly selective portion of the receptor amino acid sequence (El Mestikawy et al, Neurosci Lett 118, 189-192, 1990). The 5-HT1A receptor-like immunoreactivity was particularly abundant in the posterior lobules (IXB-X) of the immature vermis where it was found in the molecular-Purkinje cell layers. Immunostaining was confined to the plasmic membrane of the Purkinje cell somas, dendrites and perhaps axons (at their somatic emergence) suggesting that 5-HT receptors might participate in non-junctional 5-HT neurotransmission in the immature cerebellum.
Matthiessen
L
L
Département de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Université Pierre et Marie Curie, Paris, France.
Kia
H K
HK
Daval
G
G
Riad
M
M
Hamon
M
M
Vergé
D
D
D000818:Animals; D002531:Cerebellum / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D011689:Purkinje Cells / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000276:immunology / Q000378:metabolism*
D011985:Receptors, Serotonin