Method for sequencing synthetic oligodeoxynucleotide phosphorothioates.
Sequencing of oligodeoxynucleotide phosphorothioate by a modified Sanger method of sequencing is described. The procedure involves ligation of synthetic oligodeoxynucleotide phosphorothioate to an oligodeoxynucleotide, referred to here as "helper oligonucleotide." The helper oligonucleotide has a region which is complementary to T7 primer. By using DNA polymerase and nucleoside triphosphate mixture, 5'-labeled T7 primer is extended onto ligated oligodeoxynucleotide phosphorothioate, which is then analyzed on gel electrophoresis.
Tang
J
J
Hybridon, Inc., Worcester, Massachusetts 01605.
Roskey
A M
AM
Agrawal
S
S
D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D015345:Oligonucleotide Probes; D017422:Sequence Analysis, DNA / Q000379:methods*; D019914:Taq Polymerase
D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase