Eosinophilia in myelodysplastic syndrome with a (12;21)(q23;q22) translocation.
Cytogenetic analysis of bone marrow (BM) cells from a patient with myelodysplastic syndrome (MDS) associated with eosinophilia showed a 45,XY,t(12;21)(q23;q22), -17 karyotype. We performed clonal and suspension cultures using the patient's BM mononuclear cells to clarify the mechanism of eosinophilia. Eosinophil colonies formed in the presence of interleukin-5 (IL-5) and granulocyte-macrophage colony-stimulating factor (GM-CSF), but not in the presence of IL-3. When BM cells were cultured in suspension in the presence of IL-5, they differentiated to mature eosinophils, and chromosome analysis identified the 45,XY,t(12;21)(q23;q22), -17 karyotype in all metaphases. The patient's serum did not stimulate eosinophil proliferation or differentiation in comparison with normal serum, however, these data suggest that the abnormal clone with 45,XY,t(12;21)(q23;q22), -17 karyotype may have an increased responsiveness to IL-5 and GM-CSF, resulting in eosinophilia.
Kobayashi
H
H
Second Department of Internal Medicine, Shinshu University School of Medicine, Nagano-Ken, Japan.
Kitano
K
K
Shimodaira
S
S
Ishida
F
F
Saito
H
H
Sonoyama
M
M
Enokihara
H
H
Furuta
S
S
D000368:Aged; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division; D002478:Cells, Cultured; D002872:Chromosome Deletion; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D002891:Chromosomes, Human, Pair 21; D004802:Eosinophilia / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D004804:Eosinophils / Q000473:pathology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D015848:Interleukin-5 / Q000502:physiology; D007621:Karyotyping; D008297:Male; D009190:Myelodysplastic Syndromes / Q000150:complications / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic
D015848:Interleukin-5; D016178:Granulocyte-Macrophage Colony-Stimulating Factor