[Use of a series of synthetic peptides and purified major histocompatibility complex I (H-2K(b)) molecules for inhibiting T-suppressors, specific for the K(b) molecule, and their induction by peptides in vivo].
Six synthetic peptides of the MHC class 1 molecule corresponding to individual H-2kb participants in amino acid sequences of domains alpha 1 (peptide 1 and 2) and alpha 2 (peptide 3, 4, 5, 6) were selected. Kb-specific suppressor T cells (Ts) were in vivo in mice, then pretreated with a set of peptides and assayed by proliferation decrease in the third-partial mixed lymphocyte culture (MLC). Effector function of Ts was abolished by the complex of the alpha 2-domain peptides (but not by the alpha 1-domain peptides) and decreased by each peptide (4, 5, 6) of the alpha 2-domain. Both alpha 1 and alpha 2 domain peptides, added at high concentrations, decreased the otherwise efficient enrichment of Ts during the absorption-elution procedure on the syngeneic macrophage (MP) monolayers. A similar significant effect was observed the purified Kb molecule (100 mg/ml) on the allogeneic MP monolayer. Interaction between Ts receptors with some MHC peptides indicates effector Ts activation in vivo by induction with peptides 5+6 of the alpha 2 domain. The fine mechanisms of interaction between MHC class I molecule epitopes and T cell receptors (TCR) of each of the T cell subsets separately are under study now.
Brondz
B D
BD
Anafalova
T V
TV
Pavlova
L S
LS
Pankratova
E V
EV
Kozhich
A G
AG
Moshnikov
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D015395:Histocompatibility Antigens Class I / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000187:drug effects*
D015395:Histocompatibility Antigens Class I; D010455:Peptides
Ispol'zovanie nabora sinteticheskikh peptidov i ochishchennoĭ molekuly glavnogo kompleksa gistosovmetimosti klassa I (H-2K(b)) dlia ingibirovaniia T-supressorov, spetsifichnykh k toĭ zhe molekule K(b), i ikh induktsiia peptidami in vivo.