The synthetic colon peptide pyroGlu-His-GlyOH inhibits growth of human colon carcinoma cells (HT-29) transplanted subcutaneously into athymic mice.
Growth inhibitory tripeptide, pyroGlu-His-GlyOH colon mitosis inhibitor (CMI), was recently isolated from water extracts of mouse intestine. In the present study, it was demonstrated in two different experiments that i.p. injections of CMI, daily or 3 times a week, could inhibit the long-term s.c. growth of HT-29 cells in athymic mice. The growth inhibitory effect was most pronounced initially with approximately 50-90% growth inhibition, and declined terminally with approximately 40-60% growth inhibition. A bell-shaped dose-response curve with maximal effect at 1 pmol/animal/injection was seen.
Paulsen
J E
JE
Institute of Pathology, National Hospital, Oslo, Norway.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D009842:Oligopeptides; C023866:pyroglutamyl-histidyl-glycine; D011761:Pyrrolidonecarboxylic Acid