title: TRP-1 expression correlates with eumelanogenesis in human pigment cells in culture.
abstract: We have investigated the relationship in human cultured normal and malignant melanocytes between the accumulation of mRNAs encoding tyrosinase and tyrosinase-related protein-1 (TRP-1), the activity of tyrosinase and the presence of melanin. Tyrosinase mRNA correlates with tyrosinase activity and with the presence of pheomelanin, eumelanin or both melanin types. In contrast TRP-1 mRNA is only detectable in cells containing eumelanin, which suggests a role for TRP-1 in the eumelanin synthesis pathway.
authors.affiliation: LOCE-Faculty of Medicine, Université Libre de Bruxelles, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: del Marmol
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: I
authors.initials: I
authors.lastname: Jackson
authors.forename: J
authors.initials: J
authors.lastname: Vachtenheim
authors.forename: P
authors.initials: P
authors.lastname: Berr
authors.forename: G
authors.initials: G
authors.lastname: Ghanem
authors.forename: R
authors.initials: R
authors.lastname: Morandini
authors.forename: K
authors.initials: K
authors.lastname: Wakamatsu
authors.forename: G
authors.initials: G
authors.lastname: Huez
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D004277:DNA, Single-Stranded; D006801:Humans; D008543:Melanins / Q000096:biosynthesis* / Q000378:metabolism; D008544:Melanocytes / Q000201:enzymology / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D004277:DNA, Single-Stranded; D008543:Melanins; D008562:Membrane Glycoproteins; D011506:Proteins; D012333:RNA, Messenger; C018362:pheomelanin; C041877:eumelanin; D010088:Oxidoreductases; C489799:TYRP1 protein, human; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase