DNA sequence of type 1 fimbrin, Fpul1, gene from a chicken pathogenic Escherichia coli serotype O78.
The gene encoding type 1 fimbriae of chicken pathogenic Escherichia coli serotype O78 (designated Fpul1) was cloned and the genetic region encoding fimbrial subunit was sequenced. The nucleotide sequence and its deduced amino acid sequence demonstrated that the Fpul1 was a novel variation among E. coli type 1 fimbriae and showed an extensive homology to previously reported Klebsiella pneumoniae type 1 fimbriae. The E. coli K-12 strains carrying the Fpul1 genes did not show the acid-induced autoagglutination, suggesting that the Fpul1 was genetically distinct from the acid-induced autoagglutination.
Sekizaki
T
T
National Institute of Animal Health, Tsukuba Science City, Japan.
Ito
H
H
Asawa
T
T
Nonomura
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002645:Chickens; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D015723:Gene Library; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D008840:Microfilament Proteins; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011201:Poultry Diseases; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D015342:DNA Probes; D004279:DNA, Viral; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009838:Oligodeoxyribonucleotides; C026742:plastin