Transcytosis of the polymeric Ig receptor requires phosphorylation of serine 664 in the absence but not the presence of dimeric IgA.
MDCK cells expressing the polymeric immunoglobulin (poly-Ig) receptor, cocultured with IgA-producing hybridoma cells, transported dimeric IgA (dIgA) from the basolateral into the lumenal compartment, where it was recovered as secretory component-dIgA complexes. The tail of the receptor was phosphorylated on serines 664 and 726. Each serine was mutated to alanine. Appearance of A726 receptor at the basolateral surface was reduced approximately 5-fold. This was accompanied by a approximately 5-fold reduction in dIgA transcytosis. Basolateral delivery of receptor was not affected by mutation A664, and in the absence of dIgA, the receptor accumulated in recycling basolateral endosomes. In coculture, however, dIgA transcytosis by A664 receptor was normal. Thus, entry of receptor into the transcytotic pathway requires Ser-664 phosphorylation only in the absence of dIgA.
Hirt
R P
RP
Swiss Institute for Experimental Cancer Research, University of Lausanne, Epalinges.
Hughes
G J
GJ
Frutiger
S
S
Michetti
P
P
Perregaux
C
C
Poulain-Godefroy
O
O
Jeanguenat
N
N
Neutra
M R
MR
Kraehenbuhl
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D016764:Cell Polarity; D002478:Cells, Cultured; D046508:Culture Techniques; D004285:Dogs; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D006825:Hybridomas; D007070:Immunoglobulin A / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D012635:Secretory Component / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D007070:Immunoglobulin A; D010446:Peptide Fragments; D011971:Receptors, Immunologic; D012635:Secretory Component; D010768:Phosphoserine