[Measurement of cutaneous blood flow by laser Doppler velocimetry. Importance of probe effect and solvent effect after topical application].
A comparative methodological study of the probe and/or vehicle effects was performed in Human volunteers with two laser Doppler velocimeters (L.D.V.): Laserflo BPM 403, T.S.I., U.S.A. and Periflux PF2B, Perimed, Sweden. Individual variations of skin blood flow are great (up to 1,400%); this could be related to spontaneous variations and/or to the occlusive effect of the probe. Skin blood flow was significantly increased in 35% of the subjects after the application of the probe alone, and in 70 to 90% of the cases after topical application of water or ethanol (20 to 50 microliters). Lag times before vasodilatation measured only when the probe was applied on the skin were between 7.4 and 27 minutes, they were reduced between 5.2 and 8.3 minutes when the vehicles were applied. These data suggest that in any protocol for L.D.V. determination an absolute control and a vehicle control should be included to evaluate objectively topical vasoactive drugs.
Guessant
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Laboratoire de Pharmacologie, Unité de Recherche en Dermopharmacologie, Châtenay-Malabry.
Marty
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JP
Dupont
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C
D000287:Administration, Topical; D000328:Adult; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D006801:Humans; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D012867:Skin / Q000098:blood supply*; D012997:Solvents / Q000008:administration & dosage / Q000494:pharmacology; D014867:Water / Q000008:administration & dosage / Q000494:pharmacology
D012997:Solvents; D014867:Water; D000431:Ethanol
Mesure du flux sanguin cutané par vélocimétrie laser Doppler. Importance de l'effet sonde et de l'effet solvant après application topique.