Characterization of the traC determinant of the Enterococcus faecalis hemolysin-bacteriocin plasmid pAD1: binding of sex pheromone.
pAD1, a conjugative, 60-kb, hemolysin-bacteriocin plasmid in Enterococcus faecalis, encodes a mating response to a small peptide sex pheromone, cAD1, secreted by potential recipient bacteria. A gene, traC, encoding a 60.7-kDa protein with a typical amino terminal signal peptide, was identified within a region that appears to encode a product that binds to exogenous pheromone. A cloned segment of DNA containing traC resulted in specific binding of cells to synthetic cAD1. The putative traC product has strong similarity to a product of the E. faecalis plasmid pCF10 as well as oligopeptide binding proteins of Escherichia coli, Salmonella typhimurium, and Bacillus subtilis.
Tanimoto
K
K
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109.
An
F Y
FY
Clewell
D B
DB
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000235:genetics; D001483:Base Sequence; D013293:Enterococcus faecalis / Q000235:genetics* / Q000378:metabolism; D036781:Fimbriae Proteins; D006459:Hemolysin Factors / Q000235:genetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D001430:Bacteriocins; D009842:Oligopeptides; D010675:Pheromones; C074790:traC protein, Plasmid F; D036781:Fimbriae Proteins; C043691:cAD1 bacterial sex hormone