title: Staphylococcosis of turkeys. 6. Development of penicillin resistance in an interfering strain of Staphylococcus epidermidis.
abstract: Staphylococcus epidermidis strain 115, used as an interfering agent to help reduce the incidence of staphylococcosis in turkeys, was converted into a penicillin- and chloramphenicol-resistant strain designated 115R. This was accomplished by introducing a plasmid carrying the beta-lactamase (penicillinase) and chloramphenicol-resistance genes into S. epidermidis 115 by electroporation. The resultant strain, 115R, was an efficient producer of beta-lactamase and had marked increased resistance to penicillin and chloramphenicol. A beta-lactamase DNA probe was used to confirm the presence of the beta-lactamase gene in strain 115R. S. epidermidis strain 115R retained the characteristics of tissue adherence, bacteriocin production, and non-virulence that were present in the original non-transformed strain 115, and in addition should theoretically remain colonized in poults following treatment with penicillin.
authors.affiliation: Department of Microbiology, Brigham Young University, Provo, Utah 84602.
authors.forename: S S
authors.initials: SS
authors.lastname: Whitehead
authors.forename: R W
authors.initials: RW
authors.lastname: Leavitt
authors.forename: M M
authors.initials: MM
authors.lastname: Jensen
mesh_terms: D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D010403:Penicillin Resistance / Q000235:genetics*; D010957:Plasmids; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013212:Staphylococcus epidermidis / Q000201:enzymology / Q000235:genetics*; D014169:Transformation, Bacterial / Q000235:genetics*; D014422:Turkeys / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases