Amiloride-dependent transport is the main mechanism implicated in sodium influx regulation in rat mast cells.
Mast cell sodium regulation is a largely unknown field. In our effort to study the mechanisms by which mast cells regulate sodium levels, we have examined the effect of amiloride and ouabain on 22Na entry in rat mast cells in isotonic and hypertonic conditions. Ouabain (0.5 mM) enhances sodium uptake by 32% in isotonic conditions. Hypertonicity increases by 400% the uptake of sodium through an amiloride (1 mM) dependent mechanism. Ouabain has no appreciable effect on the entry of 22Na in hypertonic conditions.
Cabado
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AG
Departamento de Fisiología, Facultad de Veterinaria, Lugo, Spain.
Vieytes
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MR
Botana
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LM
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D006982:Hypertonic Solutions / Q000494:pharmacology; D007552:Isotonic Solutions / Q000494:pharmacology; D008407:Mast Cells / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000493:pharmacokinetics*
D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D010042:Ouabain; D000584:Amiloride; D012964:Sodium