title: Identity of Escherichia coli tRNA(Cys) determined by nucleotides in three regions of tRNA tertiary structure.
abstract: The in vivo aminoacylation specificity of tRNA(Cys) was studied with mutants of opal and amber suppressor tRNAs in Escherichia coli. This specificity depends not only on nucleotides in the acceptor end and anticodon of tRNA(Cys), but also on nucleotides in the dihydrouridine stem and loop and the variable loop that interact, forming the complex core of tRNA tertiary structure.
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison 53706-1567.
authors.forename: W H
authors.initials: WH
authors.lastname: McClain
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D016153:Genes, Suppressor; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012350:RNA, Transfer, Cys / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012353:RNA, Transfer, Gly / Q000737:chemistry / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012984:Software
chemical_list: D012350:RNA, Transfer, Cys; D012353:RNA, Transfer, Gly