The biosynthetic genes for clavulanic acid and cephamycin production occur as a 'super-cluster' in three Streptomyces.
The cosmid cloning vector pHC79 has been used to clone fragments of chromosomal DNA from the Streptomyces: S. clavuligerus, S. jumonjinensis and S. katsurahamanus. These strains all produce both the beta-lactam antibiotic, cephamycin and the beta-lactamase inhibitor, clavulanic acid. Although structurally related these two beta-lactams are known to be derived from different biosynthetic precursors. Hybridisation studies and restriction mapping have shown that the gene clusters encoding the two biosynthetic pathways are chromosomally adjacent in these strains, thus creating a 'super-cluster' of genes involved in both the production and enhancement of activity of a beta-lactam antibiotic.
Ward
J M
JM
SmithKline Beecham Pharmaceuticals, Brockham Park Research Centre, Betchworth, Surrey, UK.
Hodgson
J E
JE
D001426:Bacterial Proteins / Q000235:genetics*; D002513:Cephamycins / Q000096:biosynthesis*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000096:biosynthesis*; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D019751:Intramolecular Transferases; D007535:Isomerases / Q000235:genetics*; D006899:Mixed Function Oxygenases / Q000235:genetics*; D005810:Multigene Family; D046915:Penicillin-Binding Proteins; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D002513:Cephamycins; D002969:Clavulanic Acids; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D019818:Clavulanic Acid; D006899:Mixed Function Oxygenases; C066046:clavaminate synthase; D007535:Isomerases; D019751:Intramolecular Transferases; C038371:deacetoxycephalosporin C synthetase; C024925:cephamycin C