title: [Detection of human parvovirus B19 DNA using PCR and serological test using ELISA for diagnosis of infection].
abstract: The development of a polymerase chain reaction (PCR) method for detection of human parvovirus B19 DNA and serological test of clinical specimens using ELISA is described. Of 43 serum (or plasma) samples, 29 were found to be positive for B19 DNA using the PCR, anti-IgM was detected in 16 specimens and anti-IgG in 25. In all specimens confirmed to contain B19-specific IgM, the presence of B19 DNA was demonstrated. Furthermore, B19 DNA was demonstrated in a patient with B19 infection for a longer period of time than was anti-IgM. In addition, we discuss the diagnosis of B19 infection using anti-IgG, in the acute stage and convalescent stage. In fetal infection, the PCR method provides sufficient detection of B19 DNA in cord blood, amniotic fluid, ascitic fluid, and fetal pericardial effusion and pleural effusion.
authors.affiliation: SRL, Inc., Hachioji Labs.
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Ikegaya
authors.forename: S
authors.initials: S
authors.lastname: Yano
authors.forename: T
authors.initials: T
authors.lastname: Yamauchi
authors.forename: K
authors.initials: K
authors.lastname: Hikiji
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D016731:Erythema Infectiosum / Q000175:diagnosis*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007223:Infant; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007075:Immunoglobulin M