Characterization of capacitation inhibitory protein from rabbit seminal plasma: homology with human annexins.
A protein was purified from rabbit seminal plasma using preparative acrylamide disc electrophoresis, ammonium sulfate precipitation and gel filtration. The protein inhibited the in vitro fertilization of mouse ova inseminated with epididymal sperm and with capacitated sperm. The inhibition was not observed, however, when only ova were exposed to the protein prior to mixing with sperm. The partial protein sequence analysis revealed the strong homology of the rabbit fertilization inhibitory protein to human annexin V.
Okabe
M
M
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Kishi
Y
Y
Ying
X
X
Kohama
Y
Y
Mimura
T
T
Li
S S
SS
D000595:Amino Acid Sequence; D000645:Ammonium Sulfate; D000818:Animals; D017302:Annexins / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005306:Fertilization / Q000187:drug effects; D005307:Fertilization in Vitro; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D011817:Rabbits; D012661:Semen / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013075:Sperm Capacitation / Q000187:drug effects
D017302:Annexins; D011506:Proteins; D000645:Ammonium Sulfate