[Detection of toxin-producing types of Pasteurella multocida--a comparison of methods].
28 Pasteurella multocida strains were examined for production of toxin by 6 different methods. Identical results were obtained using a mice lethality test, a tissue cell culture assay and an ELISA. Different results were received with a dot-blot-immunoassay (1 strain), using a gene probe (6 strains) and a guinea pig skin test (8 strains). Corresponding differences were detected with 2 strains only. Tissue culture, ELISA and dot-blot-immunoassay are effective methods for the diagnosis of toxin-producing Pasteurella multocida strains. Animal experiments should be an exception.
Erler
W
W
Institut für Veterinärmedizin, Bundesgesundheitsamtes, Bereich Jena.
Hänel
I
I
Hotzel
H
H
Müller
W
W
Schimmel
D
D
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001681:Biological Assay; D002460:Cell Line; D003885:Dermotoxins / Q000096:biosynthesis*; D006168:Guinea Pigs; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D051379:Mice; D009693:Nucleic Acid Hybridization; D016979:Pasteurella multocida / Q000302:isolation & purification / Q000378:metabolism*; D012882:Skin Tests
D001427:Bacterial Toxins; D003885:Dermotoxins; C052804:dermonecrotic toxin, Pasteurella multocida
Nachweis toxinbildender Stämme von Pasteurella multocida--ein Vergleich von Methoden.