The major capsid protein VP2 of minute virus of mice (MVM) can form particles which bind to the 3'-terminal hairpin of MVM replicative-form DNA and package single-stranded viral progeny DNA.
The capsids of minute virus of mice (MVM) consist of two closely related proteins, VP1 and VP2. We inactivated the VP1 gene in an infectious clone of MVM DNA by frameshift mutation. After transfection of mutated DNA, capsids consisting of VP2 only were made. They can package negative-strand DNA, and they specifically bind MVM 3'-terminal hairpin DNA.
Willwand
K
K
Swiss Institute for Experimental Cancer Research, Epalinges.
Hirt
B
B
D000818:Animals; D002213:Capsid / Q000096:biosynthesis / Q000378:metabolism*; D036022:Capsid Proteins; D002460:Cell Line; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D016368:Frameshift Mutation; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008915:Minute Virus of Mice / Q000378:metabolism / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011485:Protein Binding; D015183:Restriction Mapping; D014162:Transfection
D036022:Capsid Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D008715:Methionine