title: Demonstration of a human urinary trypsin inhibitor (urinastatin)-like substance in the murine brain.
abstract: A human urinary trypsin inhibitor (urinastatin)-like immunoreactive substance with trypsin-inhibitory activities was found in the murine brain. Regional levels of this urinastatin (UT)-like substance in the brain were within 5 ng/mg protein and were expressed in a descending order as follows: cerebral cortex not equal to hippocampus > hypothalamus > mesencephalon not equal to corpus striatum >> medulla oblongata > cerebellum. Mechanical lesioning of the cerebral cortex or hippocampus with a needle induced a rapid and intense appearance of a UT-like immunoreactive substance in the neuronal cells of injured sites. Conditioned fear-stress induction incited a reversible increase in the level of UT-like immunoreactive substance in the hippocampus. These results suggest that the UT-like immunoreactive substance is produced by neurons in response to brain injury and fear-stress stimuli.
authors.affiliation: Department of Pharmacology, Shimane Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shikimi
authors.forename: T
authors.initials: T
authors.lastname: Wessel
authors.forename: T H
authors.initials: TH
authors.lastname: Joh
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Kaneto
authors.forename: K
authors.initials: K
authors.lastname: Hattori
authors.forename: S
authors.initials: S
authors.lastname: Takaori
mesh_terms: D001921:Brain / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005239:Fear; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D013315:Stress, Psychological / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000032:analysis / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D014361:Trypsin Inhibitors; C028665:urinastatin