title: A ribosomal protein in Acanthamoeba polyphaga is conserved in eukaryotic nuclei, organelles and bacteria.
abstract: An EcoRI fragment from the mitochondrial DNA of Acanthamoeba polyphaga was cloned and partly sequenced, and the conceptual translation product of the open reading frame (partial sequence) was found to have similarities with rp114, a ribosomal protein. Phylogenetic analysis based on the amino acid (aa) sequences of this conserved protein resolved four branches that consisted of: (1) eubacteria and the chloroplasts of algae and higher plants, (2) ciliate mitochondria, (3) Acanthamoeba, and (4) archaebacteria and the nuclei of eukaryotes. The groupings based on the rp114 aa sequences were consistent with the phylogenies derived by rRNA analysis of these organisms.
authors.affiliation: Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
authors.forename: M H
authors.initials: MH
authors.lastname: Vodkin
authors.forename: V R
authors.initials: VR
authors.lastname: Gordon
authors.forename: G L
authors.initials: GL
authors.lastname: McLaughlin
mesh_terms: D000048:Acanthamoeba / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000737:chemistry / Q000235:genetics; D001419:Bacteria / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002736:Chloroplasts / Q000737:chemistry; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D005057:Eukaryotic Cells / Q000737:chemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004272:DNA, Mitochondrial; D012269:Ribosomal Proteins; C041174:ribosomal protein L14