Origin of glutaminyl-tRNA synthetase: an example of palimpsest?
Sequence data and evolutionary arguments suggest that a similarity may exist between the C-terminal end of glutaminyl-tRNA synthetase (GlnRS) and the catalytic domain of glutamine amidotransferases (GATs). If true, this would seem to imply that the amidation reaction of the Glut-tRNA(Gln) complex was the evolutionary precursor of the direct tRNA(Gln) aminoacylation pathway. Since the C-terminal end of GlnRS does not now have an important functional role, it can be concluded that this sequence contains vestiges that lead us to believe that it represents a palimpsest. This sequence still conserves the remains of the evolutionary transition: amidation reaction-->aminoacylation reaction. This may be important in deciding which mechanism gave origin to the genetic code organization. These observations, together with results obtained by Gatti and Tzagoloff [J. Mol. Biol. (1991) 218:557-568], lead to the hypothesis that the class I aminoacyl-tRNA synthetases (ARSs) may be homologous to the GATs of the trpG subfamily, while the class II ARSs may be homologous to the GATs of the purF subfamily. Overall, this seems to point to the existence of an intimate evolutionary link between the proteins involved in the primitive metabolism and aminoacyl-tRNA synthetases.
Di Giulio
M
M
International Institute of Genetics and Biophysics, CNR, Naples, Napoli, Italy.
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry*; D000878:Anthranilate Synthase; D001665:Binding Sites; D005075:Biological Evolution; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D019884:Nitrogenous Group Transferases; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D014166:Transferases / Q000737:chemistry
D014166:Transferases; D019884:Nitrogenous Group Transferases; D000878:Anthranilate Synthase; C020655:anthranilate synthase, glutamine amidotransferase subunit; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase