Purification of PVYo and its soluble antigen by physico chemical means.
Potato virus Yo was purified by centrifugation of infected and minced plant tissue in the virus extraction rotor. As the initial seeding material was heavily contaminated with tobacco mosaic virus (TMV) this virus was isolated and antibody was elicited in chickens. The chicken antibody (IgY) against TMV was used for removing this extraneous virus from the original PVYo seeding material prior to propagating PVYo in tobacco plants, CV Glutinosa.
Polson
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Department of Biochemistry, University of Stellenbosch, Tygerberg, Republic of South Africa.
D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D002498:Centrifugation; D004586:Electrophoresis / Q000379:methods; D007136:Immunoglobulins / Q000276:immunology; D010942:Plant Viruses / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D011233:Precipitin Tests / Q000379:methods; D011198:Solanum tuberosum / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000276:immunology / Q000302:isolation & purification
D000956:Antigens, Viral; C016512:IgY; D007136:Immunoglobulins