Identification of human DNA in complex biological samples using the Alu polymerase chain reaction.
Alu-Polymerase chain reaction (PCR) was used to amplify human DNA from complex mixed sources of DNA. Amplification of human DNA sequences by Alu-PCR could be accomplished in samples containing low concentrations of template in the presence of excess heterologous DNA sequences. Thus, sensitivity and specificity are maintained in complex DNA mixtures allowing positive identification of the presence of human DNA sequences by this technique.
Tsongalis
G J
GJ
Department of Pathology, University of North Carolina, Chapel Hill.
Coleman
W B
WB
Esch
G L
GL
Smith
G J
GJ
Kaufman
D G
DG
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004847:Epithelial Cells; D005347:Fibroblasts; D006801:Humans; D008099:Liver; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats
D004269:DNA, Bacterial; D004247:DNA