Effects of daunomycin and its macromolecular analog daunophilin on the proliferation of mammalian cells.
Effects of daunomycin and daunomycin bound to an HPMA copolymer (daunophilin) on the proliferation of mammalian cells cultivated in vitro were compared. One line of non-tumor cells (LEP human cells) and two tumor cell lines (human HeLa cells and C6 rat cells) were used. It was found that both daunomycin and daunophilin had an inhibitory effect on the proliferation of the three cell lines and that the effect was irreversible. Daunophilin had to be used in concentrations by two orders of magnitude higher (> 1 micrograms/ml) than daunomycin (> 0.01 micrograms/ml) to produce comparable effect on cell growth.
Keprtová
J
J
Institute of Biophysics, Czech Academy of Sciences, Brno.
Vyhnáková
M
M
Minárová
E
E
Kleinwächter
V
V
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003630:Daunorubicin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005910:Glioma / Q000473:pathology; D006367:HeLa Cells; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000196:embryology; D008689:Methacrylates / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured
D008689:Methacrylates; C082679:daunophilin; C032802:hydroxypropyl methacrylate; D003630:Daunorubicin