title: Major chromosomal length polymorphisms are evident after meiosis in the phytopathogenic fungus Leptosphaeria maculans.
abstract: Chromosomal DNA of Australian field-isolates of the phytopathogenic ascomycete Leptosphaeria maculans was resolved by pulsed-field gel electrophoresis. All isolates examined had highly variable karyotypes. Ascospores (sexual spores) derived from single pseudothecia (sexual fruiting bodies) isolated from Brassica napus (oilseed rape) stubble were analyzed. In two tetrads four distinct karyotypes were observed, with only one chromosomal DNA band in common to all the members of each tetrad. Although isolates had highly variable karyotypes, two overall patterns were present. In one pattern there were at least 12 chromosomal DNA bands, the largest being greater than 2.2 Mb in size; in the other there were more than 15 chromosomal DNA bands, the largest being about 2.0 Mb. The chromosomal DNA preparations included mitochondrial DNA which migrated as a diffuse band between 0.10 and 0.15 Mb in size, and DNA molecules of 8 and 9 kb in size.
authors.affiliation: Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: K M
authors.initials: KM
authors.lastname: Plummer
authors.forename: B J
authors.initials: BJ
authors.lastname: Howlett
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D001315:Australia; D001483:Base Sequence; D015825:Chromosomes, Fungal; D004271:DNA, Fungal; D016521:Electrophoresis, Gel, Pulsed-Field; D007621:Karyotyping; D008540:Meiosis; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D004271:DNA, Fungal