Cloning and expression in Escherichia coli of Thermotoga neapolitana genes coding for enzymes of carbohydrate substrate degradation.
A genomic library of thermophilic anaerobic eubacterium Thermatoga neapolitana was constructed in E. coli using the pTZ19R plasmid vector. Some groups of recombinant clones with different cellulase activities were revealed: clones carrying genes for an 1,4-beta-glucanases, 1,3-beta-glucanases, beta-xylanases, beta-glucosidases and beta-xylosidases. One clone possessing avicelase activity was obtained. Some clones were selected with amylolytic activities toward amylose, amylopectin and pullulan.
Dakhova
O N
ON
Institute of Molecular Genetics, Russian Academy of Science, Moscow.
Kurepina
N E
NE
Zverlov
V V
VV
Svetlichnyi
V A
VA
Velikodvorskaya
G A
GA
D002480:Cellulase / Q000235:genetics; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial / Q000235:genetics*; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000235:genetics; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology / Q000235:genetics*; D006358:Hot Temperature; D011134:Polysaccharides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013213:Starch / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics
D011134:Polysaccharides; D011994:Recombinant Proteins; D002482:Cellulose; D013213:Starch; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D002480:Cellulase