Primary structure of scombrine gamma, protamine isolated from spotted mackerel (Scomber australasicus).
Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombrine I gave three bands upon PAGE. Scombrine II (scombrine gamma) consists of 34 amino acid residues, and its sequence is: Pro-Arg-Arg-Arg-Arg-Arg-Ala-Ser-Arg-Pro-Val-Arg-Arg-Arg-Arg-Arg- Ala-Arg-Arg-Ser-Thr-Ala-Val-Arg-Arg-Arg-Arg-Arg-Val-Val-Arg-Arg-Arg-Arg. The ion spray mass spectrum shows that scombrine gamma has a molecular mass of 4,532.13 Da. The other minor component (scombrine I) is considered to be a mixture of degradation products of scombrine gamma based on the results of PAGE and ion spray mass spectrometry. Scombrine gamma has a similar sequence to sardaine Z2 from striped bonito except for two positions.
Okamoto
Y
Y
Department of Biochemistry, Daiichi College of Pharmaceutical Sciences, Fukuoka.
Ogawa
K
K
Motohiro
T
T
Nishi
N
N
Muta
E
E
Ota
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D005399:Fishes; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010434:Pepsin A; D010802:Phylogeny; D011479:Protamines / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D011479:Protamines; C083061:scombrine; D010434:Pepsin A