Plasma levels of superoxide dismutase and its isomers in patients with chronic renal disease.
To evaluate the significance of Cu,Zn-superoxide dismutase (SOD) in chronic renal disease, we evaluated the plasma SOD activity and analyzed the plasma Cu,Zn-SOD isomers employing gel column chromatography. The plasma SOD activity was determined as the biological activity using the nitrite method and the Cu,Zn-SOD concentration was assayed from the immunological activity using enzyme-linked immunosorbent assay (ELISA). The subjects comprised 185 patients with chronic glomerular disease and 20 hemodialysis patients. Plasma from 170 healthy persons was employed as a control. Both the plasma biological activity and plasma level of Cu,Zn-SOD determined by ELISA were elevated in patients with chronic glomerular disease. In hemodialysis patients, a marked increase in Cu,Zn-SOD level (ELISA) was noted in comparison with the increase in SOD biological activities. Gel column chromatography demonstrated a marked increase in Cu,Zn-SOD monomer which was enzymatically inactive. From these results, we conclude that a marked elevation of the plasma level of Cu,Zn-SOD in hemodialysis patients was caused by an increase in the enzymatically inactive Cu,Zn-SOD monomer.
Futenma
A
A
First Department of Internal Medicine, Aichi Medical University, Japan.
Yamada
H
H
Kitano
M
M
Miyai
H
H
Fukatsu
A
A
Kato
K
K
D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007676:Kidney Failure, Chronic / Q000201:enzymology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D013482:Superoxide Dismutase / Q000097:blood*
D007527:Isoenzymes; D013482:Superoxide Dismutase