The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44.
Invariant chain (Ii) is a nonpolymorphic glycoprotein that associates with major histocompatibility complex class II molecules and has been shown to mediate several functions in class II-restricted antigen presentation. A small proportion of Ii is modified by the addition of chondroitin sulfate (Ii-CS), and this form of Ii is associated with class II on the surface of antigen-presenting cells. In this report we show that expression of Ii-CS dramatically enhanced the ability of class II-positive EL4 transfectants to stimulate class II-dependent allogeneic and mitogenic T cell responses. Antibody blocking studies and the ability of CD44 to bind directly to Ii-CS suggest that Ii-CS can function as an accessory molecule during T cell responses through interactions with CD44.
Naujokas
M F
MF
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Morin
M
M
Anderson
M S
MS
Peterson
M
M
Miller
J
J
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte; D001692:Biological Transport; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008643:Mesentery / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D017510:Protein Folding; D016201:Receptors, Lymphocyte Homing / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
D000944:Antigens, Differentiation, B-Lymphocyte; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D016201:Receptors, Lymphocyte Homing; C045084:invariant chain; D002809:Chondroitin Sulfates