title: Deposition of reactive oxygen metabolites onto and within living tumor cells during neutrophil-mediated antibody-dependent cellular cytotoxicity.
abstract: In this study we test the hypothesis that reactive oxygen metabolites are delivered from neutrophils to simultaneously both the cell surface and cytosol of opsonized YAC erythroleukemic target cells. Using 5' (or 6') carboxyl-2',7'-dichlorodihydrofluorescein (H2-CDCF) diacetate as starting material, we synthesized its succinimidyl ester derivative. H2-CDCF-conjugated IgG prepared from the succinimidyl ester derivative was used to opsonize targets. In vitro studies have shown that H2-CDCF becomes fluorescent upon exposure to reactive oxygen metabolites, including hydrogen peroxide. Using video intensified epifluorescence microscopy, we observed that reactive oxygen metabolites are deposited on tumor cell membranes during neutrophil-mediated antibody-dependent cellular cytotoxicity (ADCC). This deposition process is catalase sensitive. The role of reactive oxygen metabolites produced by neutrophils in triggering the oxidation of H2-CDCF is further supported by the observation that neutrophils from chronic granulomatous disease (CGD) patients did not affect target fluorescence. YAC tumor cells were also labeled with dihydrorhodamine 123 or dihydrotetramethylrosamine. The oxidized forms of these reagents were found within the cytoplasm of YAC cells. During ADCC normal neutrophils, but not neutrophils obtained from CGD patients, triggered the oxidation of dihydrorhodamine 123 and dihydrotetramethylrosamine within tumor cells. Using two-color automated epifluorescence microscopy, we could not detect temporal intermediates with fluorescence in only one compartment, i.e., either solely on the plasma membrane or in the cytoplasm. These observations suggest that reactive oxygen metabolites cross target membranes (< 12 sec. These studies show that reactive oxygen metabolites are deposited both onto and into tumor cells during ADCC, wherein both compartments could become vulnerable to oxidant-mediated damage.
authors.affiliation: Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202.
authors.forename: D
authors.initials: D
authors.lastname: Cao
authors.forename: L A
authors.initials: LA
authors.lastname: Boxer
authors.forename: H R
authors.initials: HR
authors.lastname: Petty
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D003593:Cytoplasm / Q000502:physiology / Q000648:ultrastructure; D005452:Fluoresceins / Q000378:metabolism; D006105:Granulomatous Disease, Chronic / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000473:pathology*; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000473:pathology / Q000502:physiology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D012235:Rhodamines / Q000378:metabolism; D013053:Spectrophotometry; D014407:Tumor Cells, Cultured
chemical_list: D005452:Fluoresceins; D017382:Reactive Oxygen Species; D012235:Rhodamines; C068447:dihydrotetramethylrosamine; C058319:dihydrorhodamine 123; C082545:5'-carboxyl-2',7'-dichlorodihydrofluorescein; C082546:6'-carboxyl-2',7'-dichlorodihydrofluorescein; D006861:Hydrogen Peroxide; D010100:Oxygen