Cloning and expression of a novel human profilin variant, profilin II.
We have isolated a 1.7 kbp cDNA encoding a 140 amino acid protein (15.1 kDa, pI 5.91) with a high sequence similarity (62%) to human profilin (profilin I). We have termed this variant profilin II. Northern blot analysis showed that profilin II is highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver. In addition, three different transcript lengths were detected. Only one transcript of profilin I was found. The expression level of this was low in brain and skeletal muscle, medium in heart and high in placenta, lung, liver and kidney.
Honoré
B
B
Institute of Medical Biochemistry, Aarhus University, Denmark.
Madsen
P
P
Andersen
A H
AH
Leffers
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003285:Contractile Proteins; D004247:DNA; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D051304:Profilins; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D003285:Contractile Proteins; D008840:Microfilament Proteins; C495738:PFN1 protein, human; C495741:PFN2 protein, human; D051304:Profilins; D012333:RNA, Messenger; D004247:DNA