CD30 antigen, a marker for Hodgkin's lymphoma, is a receptor whose ligand defines an emerging family of cytokines with homology to TNF.
CD30 is a surface marker for neoplastic cells of Hodgkin's lymphoma and shows sequence homology to members of the tumor necrosis factor (TNF) receptor superfamily. Using a chimeric probe consisting of the extracellular domain of CD30 fused to truncated immunoglobulin heavy chains, we expression cloned the cDNA cognate from the murine T cell clone 7B9. The encoded protein is a 239 amino acid type II membrane protein whose C-terminal domain shows significant homology to TNF alpha, TNF beta, and the CD40L. Cross-hybridization to an induced peripheral blood T cell cDNA library yielded the human homolog, which is 72% identical at the amino acid level. The recombinant human ligand enhances the proliferation of CD3-activated T cells yet induces differential responses, including cell death, in several CD30+ lymphoma-derived clones. The human and murine genes map to 9q33 and the proximal region of chromosome 4, respectively.
Immunex Research and Development Corporation, Seattle, Washington 98101.
C A
CA
Smith
H J
HJ
Gruss
T
T
Davis
D
D
Anderson
T
T
Farrah
E
E
Baker
G R
GR
Sutherland
C I
CI
Brannan
N G
NG
Copeland
N A
NA
Jenkins
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000378:metabolism*; D001483:Base Sequence; D001667:Binding, Competitive; D014408:Biomarkers, Tumor / Q000378:metabolism; D053285:CD30 Ligand; D002874:Chromosome Mapping; D016207:Cytokines / Q000737:chemistry / Q000235:genetics*; D006689:Hodgkin Disease / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D017730:Ki-1 Antigen; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000235:genetics*
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D053285:CD30 Ligand; D016207:Cytokines; D017730:Ki-1 Antigen; D008024:Ligands; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; C505774:TNFSF8 protein, human; C505775:Tnfsf8 protein, mouse; D014409:Tumor Necrosis Factor-alpha