[An ultramicro method for determining pyrophosphatase using malachite green B].
A modified sensitive micromethod is described for estimation of pyrophosphatase activity both in homogenate and suspensions of nuclear and mitochondrial fractions of rat liver tissue using a kit of reagents containing malachite green B. The analysis may be carried out in the preparation containing only 0.2 mg of biological material; the procedure is reproducible, rapid and allows to the requisite amount of reagents to be decreased. The activity of pyrophosphatase in homogenate, nuclear and mitochondrial fractions of rat liver tissue was higher at acid pH values as compared with that in the neutral medium; the highest enzymatic activity was detected in the nuclear fraction.
Gribanov
G A
GA
Pankrushina
A N
AN
Kirillova
E N
EN
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D004396:Coloring Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012394:Rosaniline Dyes / Q000737:chemistry*
D004396:Coloring Agents; D012394:Rosaniline Dyes; C005095:malachite green; D011755:Pyrophosphatases