Isolation of a repeated sequence from the genome of Bordetella parapertussis with characteristics of an insertion sequence element.
A repeated DNA sequence in the genome of Bordetella parapertussis was identified by means of heteroduplex formation and S1 nuclease treatment. Cloning and sequencing of the repeated sequence, revealed that it comprised 1306 basepairs, contained inverted repeats at its termini, and two large open reading frames. This insertion sequence element was designated IS1001, and was found in about 20 copies in 10 B. parapertussis strains analyzed. Identical hybridization patterns were observed in B. parapertussis when IS1001 was used as a probe. B. pertussis strains did not hybridize to IS1001.
van der Zee
A
A
Laboratory of Medical Microbiology, St. Elisabeth Hospital, Tillburg, The Netherlands.
Agterberg
C
C
van Agterveld
M
M
Peeters
M
M
Mooi
F
F
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001884:Bordetella / Q000235:genetics* / Q000302:isolation & purification; D001886:Bordetella pertussis / Q000235:genetics; D003001:Cloning, Molecular; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial