Expression of angiotensin I converting enzyme mRNA in rabbit gastric epithelial cells.
Angiotensin I converting enzyme (ACE) is a dipeptidyl carboxypeptidase synthesized by endothelial cells from many vascular beds as well as by extravascular tissues. Two forms of ACE have been characterized, a pulmonary form and a testicular form. Previously, in the gastrointestinal tract, we localized ACE in the rabbit gastric fundic tissue. In the present study, Northern blot analysis demonstrated the expression of a 5 kb ACE mRNA in fundic mucosa, identical in size to pulmonary ACE mRNA. In order to confirm the epithelial origin of this ACE, we have purified fundic epithelial cells by a flow cytometry technique by which endothelial cells were excluded and the population was enriched in intermediate and chief cells. Using reverse transcription and polymerase chain reaction with specific oligonucleotides, we have amplified from the enriched fundic epithelial cell RNA a 874 bp fragment, the restriction map of which is identical to that of rabbit lung. These findings demonstrate that in gastric mucosa ACE is expressed in fundic epithelial cells and seems to be similar to the pulmonary form.
INSERM U. 300, Faculté de Pharmacie, Montpellier, France.
I
I
Nonotte
M F
MF
Laliberté
C
C
Duperray
F
F
Hollande
J P
JP
Bali
F
F
Laliberté
C
C
Chevillard
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002469:Cell Separation; D004790:Enzyme Induction; D004848:Epithelium / Q000201:enzymology; D005434:Flow Cytometry; D005748:Gastric Fundus / Q000201:enzymology*; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000201:enzymology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D007703:Peptidyl-Dipeptidase A / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D014158:Transcription, Genetic
D007527:Isoenzymes; D012333:RNA, Messenger; D007703:Peptidyl-Dipeptidase A