Incorporation of [3H]inositol into phosphoinositides and inositol phosphates by rabbit blastocysts.
Preimplantation rabbit embryos collected at the early morula stage were cultured to blastocysts in the presence of [3H]inositol. The blastocysts were lysed, and both the aqueous and lipid portions were analysed for incorporated radioactivity. Thin-layer chromatographic separation of the lipid portion indicated that [3H]inositol was incorporated into phosphatidylinositol, phosphatidylinositol 4-phosphate, and phosphatidylinositol 4,5-bisphosphate. HPLC anion-exchange chromatography indicated that [3H]inositol was incorporated into inositol phosphates, including the two second messengers, inositol 1,4,5-triphosphate and inositol 1,3,4,5-tetrakisphosphate, and also inositol monophosphate and inositol 1,4-bisphosphate. These results provide evidence that rabbit blastocysts may have an active phosphatidylinositol second messenger system, which may be responsive to intrauterine factors or intraembryonic paracrine factors.
Fahy
M M
MM
Department of Physiology, University College, Galway, Ireland.
Kane
M T
MT
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism*; D007295:Inositol Phosphates / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000096:biosynthesis; D011817:Rabbits; D015290:Second Messenger Systems / Q000502:physiology
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D007294:Inositol