Interaction of vitamin C and vitamin E during free radical stress in plasma: an ESR study.
To study the interaction of the antioxidant vitamins C and E in a biological system, we used electron spin resonance (ESR) spectroscopy to make serial measurements of ascorbate and tocopheroxyl free radicals in plasma subjected to continuous free radical-mediated oxidative stress. Upon initiation of a continuous oxidative stress, we observed an immediate increase in the concentration of ascorbate radical, which reached a peak, and then steadily declined. Only after the virtual disappearance of the ascorbate radical did we observe the appearance of the tocopheroxyl radical. These data are consistent with the hypothesis that ascorbate is the terminal small-molecule antioxidant in biological systems. This is the first experimental demonstration that the predicted thermodynamic hierarchy of ascorbate, alpha-tocopherol, and their free radicals holds in a biological system containing endogenous levels of these antioxidant vitamins.
Sharma
M K
MK
Department of Internal Medicine, University of Iowa, Iowa City 52242.
Buettner
G R
GR
D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood* / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D010949:Plasma / Q000378:metabolism; D013312:Stress, Physiological / Q000097:blood; D014810:Vitamin E / Q000097:blood* / Q000737:chemistry
D000975:Antioxidants; D005609:Free Radicals; D014810:Vitamin E; D001205:Ascorbic Acid