Production of recombinant Xenopus fibroblast growth factor receptor-1 using a baculovirus expression system.
A baculovirus expression system has been used to express large quantities of full-length, biologically active Xenopus fibroblast growth factor receptor-1. Recombinant XFGFR-1 was purified to near homogeneity by a single step immunoaffinity purification procedure. The recombinant XFGFR-1 binds both FGF-1 and FGF-2 with high affinity. XFGFR-1 undergoes autophosphorylation principly on tyrosine residues in an immune-complex kinase assay. Thus, recombinant XFGFR-1 expressed in insect cells is biologically active and suitable for structural and functional analysis.
Dept. of Molecular Biology, Holland Laboratory, American Red Cross, Rockville, MD 20855.
S A
SA
Brown
R
R
Friesel
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D001667:Binding, Competitive; D002460:Cell Line; D002846:Chromatography, Affinity; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009036:Moths; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D014162:Transfection; D014981:Xenopus
D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1