An extended primer set for PCR amplification of murine kappa variable regions.
The design and successful usage of an extended primer set for the PCR amplification of murine variable kappa light chain sequences from mouse hybridomas are described. Since some of these primer pairs also amplify the endogenous SP2/0 aberrant light chain sequence, strategies to distinguish the irrelevant SP2/0 from the sequence of interest are also provided.
Leung
S O
SO
Garden State Cancer Center, Center for Molecular Medicine and Immunology, Newark, NJ.
Dion
A S
AS
Pellegrini
M C
MC
Goldenberg
D M
DM
Hansen
H J
HJ
D000818:Animals; D001483:Base Sequence; D006825:Hybridomas / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction
D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D009838:Oligodeoxyribonucleotides