Identification and oligosaccharide structure analysis of rhodopsin glycoforms containing galactose and sialic acid.
The N-linked oligosaccharides of frog (Rana pipiens) rhodopsin were analysed by sequential exoglycosidase digestion and gel filtration chromatography, following reductive tritiation. In addition, selected tryptic glycopeptides obtained from frog retinal rod outer segment membranes were examined by electrospray mass spectrometry (ES-MS), fast atom bombardment mass spectrometry (FAB-MS), amino acid sequence and composition analysis, and carbohydrate composition analysis. The amino acid sequence data demonstrated that the glycopeptides were derived from rhodopsin and confirmed the presence of two N-glycosylation sites, at residues Asn2 and Asn15. The predominant glycan (approximately 60% of total) had the structure GlcNAc beta 1-2Man alpha 1-3(Man alpha 1-6) Man beta 1-4GlcNAc beta 1-4GlcNAc-(Asn), with the remaining structures containing 1-3 additional hexose residues, as reported previously for bovine rhodopsin. Unlike bovine rhodopsin, however, a sizable fraction of the total glycans of frog rhodopsin also contained sialic acid (NeuAc), with the sialylated oligosaccharides being present exclusively at the Asn2 site. FAB-MS analysis of oligosaccharides released from the Asn2 site gave, among other signals, an abundant quasimolecular ion corresponding to a glycan of composition NeuAc1Hex6HexNAc3 (where Hex is hexose and HexNAc is N-acetylhexosamine), consistent with a hybrid structure. The potential biological implications of these results are discussed in the context of rod outer segment membrane renewal.
Duffin
K L
KL
Monsanto Corporate Research, St Louis, MO 63198.
Lange
G W
GW
Welply
J K
JK
Florman
R
R
O'Brien
P J
PJ
Dell
A
A
Reason
A J
AJ
Morris
H R
HR
Fliesler
S J
SJ
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001216:Asparagine / Q000378:metabolism; D002240:Carbohydrate Sequence; D005690:Galactose / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000737:chemistry*; D010084:Oxidation-Reduction; D011499:Protein Processing, Post-Translational; D011894:Rana pipiens; D012243:Rhodopsin / Q000737:chemistry* / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D012794:Sialic Acids; D001216:Asparagine; D012243:Rhodopsin; D006026:Glycoside Hydrolases; D014357:Trypsin; D019158:N-Acetylneuraminic Acid; D005690:Galactose