Saccharomyces cerevisiae multifunctional protein RAP1 binds to a conserved sequence in the Polyoma virus enhancer and is responsible for its transcriptional activity in yeast cells.
The Polyoma virus enhancer (A + B domain) activates transcription in Saccharomyces cerevisiae when joined to appropriate yeast promoter elements. We demonstrate by DNase I footprints and inhibition of binding by specific antibody, that the yeast protein RAP1 binds to the B-domain of the Polyoma enhancer and, at least in some promoter contexts, is responsible for transcriptional activity of the enhancer B-domain in yeast. Close matches to a consensus RAP1-binding site are also present in other viral enhancers.
Dipartimento di Genetica e Biologia Generale e Molecolare, Facoltà di Scienze Università di Napoli Federico II, Italy.
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Pollice
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Ciaramella
J F
JF
Pulitzer
D001483:Base Sequence; D015152:Blotting, Northern; D017124:Conserved Sequence; D004271:DNA, Fungal; D004279:DNA, Viral / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005656:Fungal Proteins / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D020668:rap GTP-Binding Proteins
D004271:DNA, Fungal; D004279:DNA, Viral; D005656:Fungal Proteins; D014157:Transcription Factors; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins