[Interaction between NAD level and rate of repair of induced DNA breaks in rat liver nuclei].
Within 3 hrs after administration of NAD into rats at a dose of 500 mg/kg of body mass more than a 3-fold increase in content of the nicotinamide in liver tissue, an increase in output of acid soluble material from DNA by 28%, 3-fold activation of the DNA reparative synthesis simultaneously with a decrease in NAD-pyrophosphorylase activity by 31% in liver tissue were detected during reparation of the DNA breaks induced after incubation with ethidium bromide under conditions of NAD excess in liver tissue. Distinct decrease in content of NAD as well as in activity of poly(ADP-ribose) polymerase was found in rat liver nuclei of both control and experimental animals during incubation of liver tissue with ethidium bromide. The rate of the DNA reparative synthesis was shown to correlate directly with the poly(ADP-ribose) polymerase activity and with the content of NAD in liver tissue.
Muliavko
N A
NA
Vasil'eva
S M
SM
Donchenko
G V
GV
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000378:metabolism / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D009243:NAD; D011065:Poly(ADP-ribose) Polymerases