Human glucagon-like peptide-1 receptor gene. Localization to chromosome band 6p21 by fluorescence in situ hybridization and linkage of a highly polymorphic simple tandem repeat DNA polymorphism to other markers on chromosome 6.
Glucagon-like peptide-1 is a fragment of proglucagon secreted by intestinal L-cells. It has potent glucose-dependent insulin secretory effects and also suppresses gastric acid secretion in the stomach. The biological actions of GLP-1 are mediated by the GLP-1 receptor, the structure of which has recently been determined. Defects in insulin secretion are a common feature of NIDDM and as such the GLP-1 receptor is a candidate for contributing to the development of this clinically and genetically heterogeneous disorder. As a first step in determining the role of the GLP-1 receptor in the development of NIDDM, we have isolated the human GLP-1 receptor gene and mapped it to chromosome 6, band p21.1, using the technique of fluorescence in situ hybridization. We also identified a simple tandem repeat DNA polymorphism in the human GLP-1 receptor gene of the form (TG)n. This DNA polymorphism has 14 alleles and a heterozygosity of > 0.8. We have used this DNA polymorphism to localize the GLP-1 receptor gene within the genetic map of the short arm of chromosome 6. This DNA polymorphism will facilitate genetic studies of the contribution of the GLP-1 receptor gene to impaired beta-cell function and NIDDM.
Howard Hughes Medical Institute, University of Chicago, Illinois.
M
M
Stoffel
R
R
Espinosa
M M
MM
Le Beau
G I
GI
Bell
D001483:Base Sequence; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D008040:Genetic Linkage; D000067757:Glucagon-Like Peptide-1 Receptor; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008126:Lod Score; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics; D018027:Receptors, Glucagon; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
C000593833:GLP1R protein, human; D000067757:Glucagon-Like Peptide-1 Receptor; D011956:Receptors, Cell Surface; D018027:Receptors, Glucagon; D004247:DNA