Evolutionary comparisons of three enzymes of the threonine biosynthetic pathway among several microbial species.
As an approach in the study of the evolution of threonine biosynthetic pathways throughout various organisms, the sequences of three enzymes, namely homoserine dehydrogenase, homoserine kinase and threonine synthase, originating from six organisms, namely Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Brevibacterium lactofermentum, Pseudomonas aeruginosa and Saccharomyces cerevisiae, were compared. As a general trend all three enzymatic activities were carried out by proteins sharing sequence relatedness (except for the homoserine kinase of P aeruginosa). Unexpectedly however, for each step one or two enzymes stood out of the main stream: i) for homoserine dehydrogenase, the yeast protein is atypically similar to the E coli enzyme; ii) for homoserine kinase, the P aeruginosa protein shares no similarity with any other species; and iii) for threonine synthase, the B subtilis protein is far distant from the enzymes of other species. Hence in contrast to other biosynthetic pathways such as the tryptophan one, the threonine pathway seems not to have evolved as a whole throughout different organisms but rather each step seems to have been subjected to multiple constraints including substrate-mediated ones and host-specific ones.
Cami
B
B
Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.
Clepet
C
C
Patte
J C
JC
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D005075:Biological Evolution; D001951:Brevibacterium / Q000201:enzymology; D019757:Carbon-Oxygen Lyases; D003352:Corynebacterium / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D006715:Homoserine Dehydrogenase / Q000737:chemistry*; D008190:Lyases / Q000737:chemistry*; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000737:chemistry*; D017853:Phosphotransferases (Alcohol Group Acceptor); D011550:Pseudomonas aeruginosa / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D013912:Threonine / Q000096:biosynthesis
D013912:Threonine; D006715:Homoserine Dehydrogenase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021370:homoserine kinase; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C023160:threonine synthase