Evidence of a link between erythrocyte band 3 phosphorylation and anion transport in patients with 'idiopathic' calcium oxalate nephrolithiasis.
This study was carried out to verify the hypothesis of a link between faster endogenous phosphorylation of band 3 protein, the anion carrier, and anomalous oxalate transmembrane self-exchange found in erythrocyte from calcium oxalate renal stone formers. Agents able to modify 32P-labelling of band 3 protein induced a concurrent modification in oxalate transmembrane flux. Cyclic AMP- and phospholipid-sensitive Ca(2+)-independent protein kinases seem to be critical modulators of band 3 function. These observations demonstrate a close link between the band 3 phosphorylation state and its anion transport function, and provide new insights into the pathogenetic mechanisms of the cellular anomalies observed in calcium-oxalate renal stone disease.
Institute of Internal Medicine, University of Padova, School of Medicine, Italy.
B
B
Baggio
L
L
Bordin
G
G
Gambaro
A
A
Piccoli
G
G
Marzaro
G
G
Clari
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D000838:Anions; D001692:Biological Transport; D002129:Calcium Oxalate / Q000032:analysis*; D000242:Cyclic AMP / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D007669:Kidney Calculi / Q000097:blood* / Q000737:chemistry; D010070:Oxalates / Q000097:blood*; D019815:Oxalic Acid; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation
D001457:Anion Exchange Protein 1, Erythrocyte; D000838:Anions; D010070:Oxalates; D010761:Phosphorus Radioisotopes; D002129:Calcium Oxalate; D019815:Oxalic Acid; D000242:Cyclic AMP