Measurement of rat brain perfusion by NMR using spin labeling of arterial water: in vivo determination of the degree of spin labeling.
In vivo NMR experiments are performed to determine the degree of spin labeling for measurement of tissue perfusion by NMR using spin labeling of arterial water by adiabatic fast passage. Arterial water spins are labeled using flow in the presence of a field gradient and B1 irradiation to fulfill the conditions for adiabatic fast passage spin inversion. It is demonstrated that the NMR-measured tissue perfusion is not affected by changing the degree of spin labeling as long as the degree of spin labeling is determined and accounted for according to the model used for calculating perfusion. By measuring the degree of spin labeling with different arterial blood flow velocities induced by different arterial pCO2, it is also demonstrated that, when spin labeling is carried out by adiabatic fast passage, the degree of spin labeling is not affected by changes in arterial blood flow velocity over a broad range.
Pittsburgh NMR Center for Biomedical Research, Pennsylvania.
W
W
Zhang
D S
DS
Williams
A P
AP
Koretsky
D000818:Animals; D001769:Blood; D001783:Blood Flow Velocity; D001834:Body Water / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002245:Carbon Dioxide / Q000097:blood; D002339:Carotid Arteries / Q000033:anatomy & histology / Q000378:metabolism; D002536:Cerebral Arteries / Q000378:metabolism* / Q000502:physiology; D002560:Cerebrovascular Circulation; D004578:Electron Spin Resonance Spectroscopy; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009333:Neck / Q000098:blood supply; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013113:Spin Labels
D013113:Spin Labels; D002245:Carbon Dioxide