Whole mouse measurement of paramagnetism-loss of nitroxide free radical in lung with a L-band ESR spectrometer.
Paramagnetism-loss of nitroxide radical in lung of whole mouse was characterized using L-band ESR technique. The nitroxide, hydroxy-TEMPO, which had been injected trans-tracheally into mouse lung was confirmed to lose its paramagnetism in one electron reduction. The reduction activity remained even after lavage of lung. The reduction was inhibited by N-ethylmaleimide, a membrane-permeable sulfhydryl-blocker, though direct reduction of the radical by sulfhydryls was not observed in vitro. Sulfhydryl-blockers floating in membranes also inhibited the reduction, depending on their chain length. The results indicate that the reduction may be done within plasma membranes of alveolar epithelial cells.
Takeshita
K
K
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Utsumi
H
H
Hamada
A
A
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003497:Cyclic N-Oxides / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D005609:Free Radicals; D008168:Lung / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D013113:Spin Labels; D005033:Ethylmaleimide; C001803:tempol