Relationship of vasopressin with NADPH-diaphorase in the hypothalamo-neurohypophysial system.
The relative anatomical distributions of vasopressin and the nitric oxide synthase, nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) were examined in the hypothalamo-neurohypophysial system using immunocytochemical and histochemical techniques. Double-labeled neurons were localized predominately to rostral aspects of the hypothalamo-neurohypophysial system. Only scattered double-labeled cells were found throughout the subdivisions of the supraoptic and paraventricular nuclei. Because previous investigations suggest that nitric oxide may play a critical role in neurotransmission and reductions in NADPH-d have been reported in the neural lobe of salt-loaded animals, the present report of its coexistence with the antidiuretic hormone vasopressin in the hypothalamo-neurohypophysial system further supports a role for these neuroactive substances in mechanisms modulating fluid homeostasis.
Research Institute, Cleveland Clinic Foundation, OH 44195.
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D000818:Animals; D007030:Hypothalamo-Hypophyseal System / Q000166:cytology* / Q000201:enzymology; D007150:Immunohistochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014667:Vasopressins / Q000032:analysis*
D014667:Vasopressins; D009252:NADPH Dehydrogenase