A model for tumor suppression using H-1 parvovirus.
A model system is proposed to investigate, at the molecular level, the pathways of tumor suppression. As a tool for the selection of cells with a suppressed phenotype, we used the H-1 parvovirus that preferentially kills various neoplastic cells. From the human K562 leukemia cells, we isolated a clone, KS, that is resistant to the cytopathic effect of the H-1 virus and displays a suppressed malignant phenotype. The suppressed malignancy and the cellular resistance to H-1 killing appear to depend on the activity of wild-type p53. Whereas the KS cells express wild-type p53, the protein is undetectable in the parental K562 cells. Experiments with p53 mutants suggest that wild-type p53, in its functionally intact state, contributes to the resistance against the cytopathic effect of H-1 parvovirus.
Telerman
A
A
Institute of Interdisciplinary Research (IRIBHN), Faculty of Medicine, Brussels, Belgium.
Tuynder
M
M
Dupressoir
T
T
Robaye
B
B
Sigaux
F
F
Shaulian
E
E
Oren
M
M
Rommelaere
J
J
Amson
R
R
D015703:Antigens, CD / Q000032:analysis; D001483:Base Sequence; D015153:Blotting, Western; D002470:Cell Survival; D002999:Clone Cells; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D016147:Genes, Tumor Suppressor; D016158:Genes, p53; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D008715:Methionine; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D009838:Oligodeoxyribonucleotides; D010321:Parvoviridae / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013489:Suppression, Genetic; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000302:isolation & purification
D015703:Antigens, CD; D004279:DNA, Viral; D009363:Neoplasm Proteins; D009838:Oligodeoxyribonucleotides; D016159:Tumor Suppressor Protein p53; D008715:Methionine