Antisense suppression of transferrin receptor gene expression in a human hepatoma cell (HuH-7) line.
A recombinant plasmid carrying human transferrin receptor cDNA in reverse orientation downstream from the human cytomegalovirus immediate early promoter/enhancer element was introduced into the HuH-7 human hepatoma cell line by lipofection. Cell surface transferrin binding and iron uptake from transferrin each decreased by about 50% in stable transfectants bearing integrated antisense DNA expression vector. Northern blot analysis indicated that the abundance of target transferrin receptor message was not altered by antisense RNA. These results suggest that the antisense transcript interferes with expression of the endogenous transferrin receptor gene at the level of translation.
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461.
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Sasaki
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Zak
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Aisen
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects*; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D011990:Receptors, Transferrin / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D016376:Oligonucleotides, Antisense; D011990:Receptors, Transferrin