Evidence for a synergistic interaction between isoprenaline and timolol on intraocular pressure in conscious rabbits.
1. Interaction between topical isoprenaline and timolol on intraocular pressure was studied in conscious rabbits through changes of the dose-response curves. 2. Both drugs caused a dose-dependent fall of intraocular pressure mainly by reducing the aqueous humour formation. 3. When combined, timolol increased the response to submaximal concentrations of isoprenaline as well as EC50 (from 2.06 x 10(-3) M to 3.63 x 10(-3) M) and slope (from 1.21 to 1.89), but peak response to isoprenaline remained unchanged. 4. Discordance between experimental and theoretical additive curves as well as an interaction index smaller than 1 show that the interaction between both drugs is synergistic.
Santafe
J
J
Departamento de Farmacología, Facultad de Farmacia, Universidad del Pais Vasco, Vitoria, Spain.
Segarra
J
J
Garrido
M
M
Pablo
V
V
Martinez de Ibarreta
M J
MJ
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D007429:Intraocular Pressure / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology*; D011817:Rabbits; D011943:Receptors, Adrenergic, beta / Q000187:drug effects; D013999:Timolol / Q000494:pharmacology*
D011943:Receptors, Adrenergic, beta; D013999:Timolol; D007545:Isoproterenol