Mutations in the Drosophila gene encoding ribosomal protein S6 cause tissue overgrowth.
We have characterized two P-element-induced, lethal mutations in Drosophila melanogaster which affect the larval hemocytes, mediators of the insect immune response. Each mutant displays larval melanotic tumors characteristic of mutations affecting the insect cellular immune system, and the moribund animals develop grossly hypertrophied hematopoietic organs because of increased cell proliferation and extra rounds of endoreduplication in some hematopoietic cells. Surprisingly, these mutations are due to P element insertions in the 5' regulatory region of the Drosophila gene encoding ribosomal protein S6 and cause a reduction of S6 transcript abundance in mutant larvae.
Center for Basic Cancer Research, Kansas State University, Manhattan 66506.
M J
MJ
Stewart
R
R
Denell
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D015870:Gene Expression; D005786:Gene Expression Regulation; D017344:Genes, Insect; D005804:Genes, Lethal; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins / Q000235:genetics*; D016415:Sequence Alignment; D014960:X Chromosome
D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins