Prenatal diagnosis of cytomegalovirus (CMV) infection: a preliminary report.
Eight patients were referred for prenatal diagnosis for suspected fetal cytomegalovirus infection (CMV): six for documented first-trimester infection and two for abnormal ultrasound evaluation suggestive of fetal infection. Three methods of diagnosis were employed: (1) amniotic fluid viral cultures and CMV-specific IgM in fetal serum; (2) amniotic fluid cultures and detection by polymerase chain reaction amplification of CMV-specific DNA in chorionic villi; and (3) detection of CMV-specific DNA in villus samples only. Amniotic fluid cultures detected all cases of infection, but CMV-specific IgM was not a reliable indicator of infection in any case. DNA analysis correlated well with both culture results and clinical outcome.
Department of Obstetrics and Gynecology, University of Maryland, Baltimore.
W A
WA
Hogge
G J
GJ
Buffone
J S
JS
Hogge
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000382:microbiology; D000914:Antibodies, Viral / Q000097:blood; D015193:Chorionic Villi Sampling; D017218:Cordocentesis; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D005315:Fetal Diseases / Q000175:diagnosis*; D006801:Humans; D015151:Immunoblotting; D007075:Immunoglobulin M / Q000097:blood; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D011296:Prenatal Diagnosis / Q000379:methods*
D000914:Antibodies, Viral; D004279:DNA, Viral; D007075:Immunoglobulin M