A survey of clinical isolates of Enterobacteriaceae using a series of DNA probes for aminoglycoside resistance genes.
DNA probes specific for the aminoglycoside adenylyltransferase ANT(2"), the aminoglycoside phosphotransferases APH(3')-I and APH(3')-II, and the aminoglycoside acetyltransferases AAC(3)-I, AAC(3)-V and AAC(6')-I were used to screen 151 clinical isolates for the presence of these resistance determinants. The two most common resistance genes in the study isolates were those encoding ANT(2") and APH(3')-I. The phenotypic pattern of aminoglycoside resistance, as determined by a modified disk diffusion test correlated well with the genotypes of the organisms as established using DNA probes, although strains carrying the ANT(2") gene could express one of several phenotypes.
Flamm
R K
RK
Seattle Veterans Affairs Medical Center, Washington 98108.
Phillips
K L
KL
Tenover
F C
FC
Plorde
J J
JJ
D000123:Acetyltransferases / Q000235:genetics*; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015342:DNA Probes; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D019868:Kanamycin Kinase; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D013045:Species Specificity
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015342:DNA Probes; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; C056532:aminoglycoside N(6')-acetyltransferase; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase