Cell type-specific interactions of transcription factors with a housekeeping promoter in vivo.
Mammalian housekeeping promoters represent a class of regulatory elements different from those of tissues-specific genes, lacking a TATA box and associated with CG-rich DNA. We have compared the organization of the housekeeping Htf9 promoter in different cell types by genomic footprinting. The sites of in vivo occupancy clearly reflected local combinations of tissue-specific and ubiquitous binding factors. The flexibility of the Htf9 promoter in acting as the target of cell-specific combinations of factors may ensure ubiquitous expression of the Htf9-associated genes.
AFRC Centre for Genome Research, University of Edinburgh, UK.
G
G
Stapleton
M P
MP
Somma
P
P
Lavia
D016475:3T3 Cells / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000737:chemistry; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000378:metabolism*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry; D008114:Liver Neoplasms, Experimental / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D013234:Stem Cells / Q000737:chemistry; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D014157:Transcription Factors; D004247:DNA