Tissue distribution of beta 3-adrenergic receptor mRNA in man.
Expression of mRNA for beta 1-, beta 2-, and beta 3-adrenergic receptors (beta 1-, beta 2-, and beta 3-AR) was investigated in human tissues. beta 1- and beta 2-AR mRNA distribution correlated with that of the cognate receptors established by pharmacological studies. beta 3-AR transcripts were abundant in infant perirenal brown adipose tissue, characterized by the presence of uncoupling protein (UCP) mRNA. In adult whole adipose tissues, beta 3-AR mRNA levels were high in deep deposits such as perirenal and omental, and lower in subcutaneous. In these deposits, UCP mRNA levels paralleled those of beta 3-AR. However, isolated omental and subcutaneous adipose cells, enriched in white adipocytes, expressed beta 3-AR but no UCP transcripts. beta 3-AR mRNA was highly expressed in gallbladder, and to a much lower extent in colon, independently of UCP mRNA. Quadriceps or abdominal muscles, heart, liver, lung, kidney, thyroid, and lymphocytes did not express intrinsic beta 3-AR mRNA. This study demonstrates that substantial amounts of brown adipocytes exist throughout life in adipose deposits, which are generally classified as white. These deposits are the main sites of beta 3-AR expression, which also occurs in gallbladder and colon. beta 3-AR may thus be involved in the control of lipid metabolism, possibly from fat assimilation in the digestive tract, to triglyceride storage and mobilization in adipose tissues.
Krief
S
S
Centre National de la Recherche Scientifíque-Unité Propre de Recherche 0415, Paris, France.
Lönnqvist
F
F
Raimbault
S
S
Baude
B
B
Van Spronsen
A
A
Arner
P
P
Strosberg
A D
AD
Ricquier
D
D
Emorine
L J
LJ
D000273:Adipose Tissue / Q000502:physiology; D000328:Adult; D000368:Aged; D001483:Base Sequence; D015152:Blotting, Northern; D002648:Child; D002675:Child, Preschool; D005260:Female; D006321:Heart / Q000502:physiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D011943:Receptors, Adrenergic, beta / Q000145:classification / Q000235:genetics*
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta