Modulation of the respiratory burst in human neutrophils by isoproterenol and dibutyryl cyclic AMP.
The effects of a beta-adrenergic agonist and a cyclic AMP analogue on activation, activity, and termination of FMLP-stimulated superoxide anion production were investigated. Incubation with isoproterenol resulted in a 50% reduction in the maximal rate of superoxide production and a 3-4-fold increase in the rate of termination of superoxide production. Exposure to 1 mM dibutyryl cyclic AMP resulted in a 40% decrease in the maximal rate and a 3-fold increase in the rate of termination of FMLP-induced superoxide production. Neither agent had a significant effect on the lag time prior to superoxide anion generation.
Department of Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, NY 14642.
K L
KL
Gibson-Berry
J C
JC
Whitin
H J
HJ
Cohen
D003994:Bucladesine / Q000494:pharmacology*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D019255:NADPH Oxidases; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011494:Protein Kinases / Q000502:physiology; D016897:Respiratory Burst / Q000187:drug effects*; D013481:Superoxides / Q000378:metabolism
D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003994:Bucladesine; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D011494:Protein Kinases; D007545:Isoproterenol