Cerium methods for light and electron microscopical histochemistry.
Methods based on the use of cerium to detect the activity of oxidases and phosphatases are rapidly expanding. Both classes of enzymes can be demonstrated with cerium at the electron and light microscopical level. The in situ detection of H2O2 production with cerium is another application that has great potential, particularly in experimental pathological research. The fine precipitate of the cerium-containing final reaction product, cerium perhydroxide or cerium phosphate, enables a very precise localization. With such techniques, important advances have been made in cell biology, such as the discovery of new organelles, functional subcompartmentization of peroxisomes, tubular lysosomes and the elucidation of the function of extracellular ATPases. At the light microscopical level, the activity of enzymes can be quantified in situ because the production of final reaction product in the cerium methods is proportional to enzyme activity in tissue sections or cells. Cerium precipitates have strong reflectance properties and this enables their use in confocal scanning laser microscopy. In the present review, the principles of cerium methods are outlined and applications in cell biology and pathology are discussed.
Laboratory of Cell Biology, University of Amsterdam, The Netherlands.
C J
CJ
Van Noorden
W M
WM
Frederiks
D000818:Animals; D002563:Cerium; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008854:Microscopy, Electron / Q000379:methods; D009504:Neutrophils / Q000201:enzymology; D010088:Oxidoreductases / Q000032:analysis*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D051381:Rats
D002563:Cerium; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D010744:Phosphoric Monoester Hydrolases