Peripheral blood lymphocytes express the platelet-type thrombin receptor.
Northern blot analysis of human mononuclear cells indicated that the platelet thrombin receptor may be expressed by lymphocytes. In order to investigate this, we prepared affinity purified rabbit antibodies against the thrombin receptor which bound platelets and blocked thrombin activation. Using flow cytometry on peripheral blood cells, we found that the vast majority of NK cells (CD16/CD56 positive) and a fraction of CD3/CD4 positive T cells expressed the thrombin receptor. B cells, neutrophils and monocytes were negative. These data suggest that potentially thrombin may play a direct role in regulating NK and T cell function.
Howells
G L
GL
Department of Oral Pathology, London Hospital Medical College.
Macey
M
M
Curtis
M A
MA
Stone
S R
SR
D000906:Antibodies / Q000276:immunology; D001667:Binding, Competitive; D015152:Blotting, Northern; D002460:Cell Line; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry; D008214:Lymphocytes / Q000737:chemistry*; D010446:Peptide Fragments / Q000276:immunology; D010974:Platelet Aggregation / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics; D018179:Receptors, Thrombin; D013601:T-Lymphocytes / Q000737:chemistry
D000906:Antibodies; D010446:Peptide Fragments; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; C072032:thrombin receptor peptide (42-55)