Differentiation of Dunn osteosarcoma cells in response to dibutyryl cyclic 3',5'-adenosine monophosphate.
We investigated the effects of dibutyryl cyclic adenosine 3',5'-monophosphate (Bt2cAMP) on the differentiation of Dunn osteosarcoma cells. Flow-cytometric analysis and DNA synthesis assay showed that Bt2cAMP decreased the cell population in the S phase in a time- and dose-dependent manner. Also, the cells showed distinct morphological and functional alterations; the cell morphology changed to a fibroblast-like appearance with long and thin protoplasmic processes, the knobs or blebs on both the cell membrane and nuclear membrane disappeared and the intracellular alkaline phosphatase activity increased. Moreover, Bt2cAMP-treated cells secreted a large quantity of fibronectin, which was deposited on the extended cell surface in the culture medium. Thus, Dunn osteosarcoma cells are differentiated morphologically and functionally by Bt2cAMP, and might be transformed to benign precursor cells.
Department of Orthopedic Surgery, Toyama Medical and Pharmaceutical University, Japan.
M
M
Kanamori
H
H
Matsui
K
K
Yudoh
A
A
Maeda
K M
KM
Kadowaki
H
H
Tsuji
H
H
Ochiai
S
S
Tatezaki
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D051379:Mice; D008809:Mice, Inbred C3H; D012516:Osteosarcoma / Q000201:enzymology / Q000473:pathology*; D014407:Tumor Cells, Cultured
D005353:Fibronectins; D003994:Bucladesine; D000469:Alkaline Phosphatase