Relationship between factors influencing Na(+)-K(+)-ATPase activity and eicosanoid production from 14C-arachidonic acid in spayed rat uterus.
Na(+)-K(+)-ATPase activity has been implicated in the metabolism of arachidonate and the release of prostaglandins (PG). The aim of the present study was to investigate a potential interaction between the activity of this enzyme and the production of bisenoic PG by uteri isolated from spayed rats. Ouabain or the incubation in low K+ medium (conditions which inhibit Na(+)-K(+)-ATPase) diminished the conversion of 14C-arachidonic acid to 6-keto-PGF1 alpha and PGF2 alpha and increased the production of TXB2. The incubation of uterine strips in a high K+ medium (condition which enhances Na(+)-K(+)-ATPase activity) increased the formation and release of 6-keto-PGF1 alpha and PGF2 alpha while the production of TXB2 and PGF2 diminished significantly. These observations suggested that the activity of Na(+)-K(+)-ATPase could modulate the production of PG and that could be involved in the alterations of the metabolism of eicosanoids found in several tissues during diabetes.
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
A M
AM
Franchi
A
A
Motta
A L
AL
Gimeno
M A
MA
Gimeno
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D015237:Dinoprost / Q000096:biosynthesis; D015777:Eicosanoids / Q000096:biosynthesis*; D005260:Female; D066298:In Vitro Techniques; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013929:Thromboxane B2 / Q000096:biosynthesis; D014599:Uterus / Q000378:metabolism*
D015777:Eicosanoids; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D010042:Ouabain; D015237:Dinoprost; D000254:Sodium-Potassium-Exchanging ATPase