The role of arginines in stabilizing the active open-lid conformation of Rhizomucor miehei lipase.
Molecular dynamics simulations for the lid covering the active site of Rhizomucor miehei lipase [EC 3.1.1.3] postulated that, among other interactions, Arg86 in the lid stabilized the open-lid conformation of the protein by multiple hydrogen bonding to the protein surface. Chemical modification of arginine residues in R. miehei lipase with 1,2-cyclohexanedione or phenylglyoxal resulted in residual activities in the hydrolysis of tributyrin of 66 and 46%, respectively. Tryptic maps of native and phenylglyoxal-reacted R. miehei lipase showed that Arg86 was the residue modified most, when the lipase was inhibited to the greatest extent. Guanidine, a structural analog to an arginine side chain, inhibited both the native enzyme and the arginine-modified enzymes, resulting in residual activities of 26% as compared to the native enzyme. The inhibition was not an effect of enzyme denaturation. The native enzyme was also inhibited by 1-ethylguanidine, benzamidine and urea, but to a lesser degree than by guanidine. Lipases from Humicola lanuginosa and porcine pancreas in 100 mM guanidine showed residual activities of 88 and 70%, respectively. The lipases from Candida antarctica, C. rugosa, Pseudomonas cepacia and P. fluorescens were not inhibited by guanidine. The inhibition of R. miehei lipase by structural analogs of the arginine side chain and after chemical modification of arginine residues suggest a role of an arginine residue in stabilizing the active open-lid conformation of the enzyme.
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
M
M
Holmquist
M
M
Norin
K
K
Hult
D000595:Amino Acid Sequence; D001120:Arginine / Q000737:chemistry; D001665:Binding Sites; D003512:Cyclohexanones; D008049:Lipase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009090:Mucorales / Q000201:enzymology* / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D010658:Phenylglyoxal; D011487:Protein Conformation
D003512:Cyclohexanones; D010446:Peptide Fragments; C018031:1,2-cyclohexanedione; D001120:Arginine; D008049:Lipase; D010658:Phenylglyoxal