The protooncogene c-sea encodes a transmembrane protein-tyrosine kinase related to the Met/hepatocyte growth factor/scatter factor receptor.
c-sea is the cellular homologue of the avian erythroblastosis virus S13-encoded oncogene v-sea. We have isolated and determined the nucleotide sequence of overlapping chicken cDNAs that encode the putative c-sea protooncogene product. The predicted reading frame encoded a 1404-aa polypeptide that had the structure of a receptor-like protein-tyrosine kinase and exhibited the highest degree of sequence similarity with the Met/hepatocyte growth factor/scatter factor receptor. Analysis of steady-state RNA expression revealed that c-sea mRNA levels were elevated approximately 5-fold in chicken embryo cells transformed by activated variants of the src nonreceptor protein-tyrosine kinase gene but not in cells transformed by the nuclear oncogenes v-myc or v-rel. A survey of c-sea expression in a variety of chicken tissues indicated that the highest levels of mRNA were located in peripheral white blood cell populations and in the intestine.
Department of Microbiology, University of Virginia, Charlottesville 22908.
J L
JL
Huff
M A
MA
Jelinek
C A
CA
Borgman
T J
TJ
Lansing
J T
JT
Parsons
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D002478:Cells, Cultured; D002642:Chick Embryo; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics*; D019859:Proto-Oncogene Proteins c-met; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000235:genetics*
D030161:Avian Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017228:Hepatocyte Growth Factor; C082018:sea protein, Gallus gallus; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met