Detection of hepatitis A virus in concentrated river water by polymerase chain reaction.
Sensitive and specific techniques are needed to detect Hepatitis A virus in environmental samples. PCR was used on concentrated river samples. In the analysis of 13 samples, two were positive by Elisa (15.3%), three were positive on cell culture (23%), five were positive by hybridization (38.4%) and height with PCR (67%).
Laboratory of Virology, Istituto Superiore di Sanità, Roma, Italy.
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Morace
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Pisani
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Divizia
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Panà
D001483:Base Sequence; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water; D006507:Hepatovirus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D014871:Water Microbiology
D015342:DNA Probes; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides