Integrin receptors and function on cultured glomerular endothelial cells.
Integrin expression and function on a cloned line of rat glomerular endothelial cells (GEndoC) were studied in an effort to obtain a better understanding of the means by which these cells interact with components of the glomerular basement membrane. Cultured GEndoC adhered to fibronectin, laminin and types I and IV collagen and expressed alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, alpha v beta 1 and alpha v beta 3 integrins. Synthetic RGDS peptides significantly decreased adhesion to fibronectin (53.1 +/- 4.7% of control). Antibody to rat beta 1 integrin strongly inhibited adhesion to laminin, fibronectin and types I and IV collagen (11.2, 19.0, 67.3 and 31.9% of control adhesion, respectively), while antibody to the rat alpha 1 integrin chain strongly inhibited adhesion to laminin (65.2% of control), but only mildly inhibited adhesion to type IV collagen (77.2% of control) and did not affect adhesion to type I collagen (97.8% of control). Affinity chromatography of GEndoC lysates on a column of immobilized type I collagen displayed predominantly binding of alpha 3 beta 1 integrin with trace amounts of alpha 1 beta 1 and alpha 2 beta 1, documenting the major role of alpha 3 beta 1 in GEndoC adhesion to collagen. Chromatography on the immobilized cell-binding fragment of fibronectin revealed the alpha 5 beta 1 integrin to be the major fibronectin receptor on these cells, but antibody to alpha v beta 3 integrin also documented a minor role for alpha v beta 1 or alpha v beta 3 in fibronectin adhesion.(ABSTRACT TRUNCATED AT 250 WORDS)
Adler
S
S
Department of Medicine, New York Medical College, Valhalla.
Eng
B
B
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology; D004727:Endothelium / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology; D005353:Fibronectins / Q000502:physiology; D016023:Integrins / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D007797:Laminin / Q000502:physiology
D005353:Fibronectins; D016023:Integrins; D007797:Laminin; D003094:Collagen