Altered protein synthesis in a cell-free system exposed to a sinusoidal magnetic field.
This report describes a new approach for examining weak extremely low frequency (ELF) electric and magnetic field interactions with living systems that exploits a cell-free transcription/translation system derived from Escherichia coli. Using two-dimensional polyacrylamide gel electrophoresis we previously had determined that the level of the alpha subunit of RNA polymerase in intact E. coli was elevated by exposure to weak ELF magnetic fields. In this paper, plasmids containing the alpha, or both the beta,beta' subunits of the RNA polymerase from E. coli were placed into a cell-free expression system. When this transcription/translation system was exposed to a 72-Hz sinusoidal magnetic field in the range 0.07 to 1.1 mT (rms) for periods of 5 min to 1 h, expression was enhanced. Weaker fields must be applied longer to produce an effect. For 10 min of field exposure, the threshold for an effect is 0.1 mT. These experiments demonstrate that an intact membrane is not an absolute requirement for transducing magnetic bio-effects.
Goodman
E M
EM
Biomedical Research Institute, University of Wisconsin-Parkside, Kenosha 53141.
Greenebaum
B
B
Marron
M T
MT
D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis; D004574:Electromagnetic Fields; D004926:Escherichia coli; D005786:Gene Expression Regulation; D010957:Plasmids; D014176:Protein Biosynthesis; D015854:Up-Regulation
D012321:DNA-Directed RNA Polymerases