Neutrophil-activating peptide (interleukin-8) in colonic mucosa from patients with Crohn's disease.
We considered the role of two neutrophil chemotactic agents (interleukin-8 and leukotriene B4) and of myeloperoxidase (a neutrophil-associated enzyme) in the pathologic condition of Crohn's disease (CD). Serial biopsy samples were taken at different sites in the colon, washed in 0.02 M phosphate-saline buffer, homogenized, and then sonicated. Interleukin-8 levels were significantly increased throughout the colonic mucosa (> 300 pg/mg protein) in patients with CD compared with control groups (< 40 pg/mg protein) (p < or = 0.01). A two- to six-fold increase in leukotriene B4 was also found in CD, whereas mucosal levels of myeloperoxidase were unchanged compared with control subjects. This study demonstrates that interleukin-8 and leukotriene B4 may have an immunologic role in the pathologic condition of CD.
Izzo
R S
RS
Dept. of Medicine, Nassau County Medical Center, East Meadow, New York.
Witkon
K
K
Chen
A I
AI
Hadjiyane
C
C
Weinstein
M I
MI
Pellecchia
C
C
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000737:chemistry*; D003424:Crohn Disease / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis*; D007413:Intestinal Mucosa / Q000737:chemistry*; D007975:Leukotriene B4 / Q000032:analysis; D008875:Middle Aged; D009195:Peroxidase / Q000032:analysis; D011863:Radioimmunoassay
D016209:Interleukin-8; D007975:Leukotriene B4; D009195:Peroxidase