Linkage analysis of fifty-seven microsatellite loci to bipolar disorder.
The authors' goal was to screen for genetic linkage with highly informative deoxyribonucleic acid (DNA) microsatellite markers on a series of moderately sized North American bipolar disorder (BP) pedigrees. These BP pedigrees were genotyped with 57 short tandem-repeat polymorphic systems (microsatellites) that were enzymatically amplified from genomic DNA. We did not find significant evidence for genetic linkage. We found isolated LOD scores greater than 2 on chromosome 1 at two loci in individual pedigrees. Simulation studies for multiple analyses under the assumptions of linkage and nonlinkage were performed. The simulations show that LOD scores greater than 2 could be expected even when linkage is absent. Significance levels need to be considered carefully in systematic linkage studies.
Gejman
P V
PV
Clinical Neurogenetics Branch, National Institute of Mental Health, Bethesda, Maryland 20892.
Martinez
M
M
Cao
Q
Q
Friedman
E
E
Berrettini
W H
WH
Goldin
L R
LR
Koroulakis
P
P
Ames
C
C
Lerman
M A
MA
Gershon
E S
ES
D001714:Bipolar Disorder / Q000235:genetics*; D002877:Chromosomes, Human / Q000648:ultrastructure; D004276:DNA, Satellite / Q000235:genetics*; D004586:Electrophoresis; D005784:Gene Amplification; D008040:Genetic Linkage / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006801:Humans; D007700:Kinetics; D008957:Models, Genetic; D011110:Polymorphism, Genetic
D004276:DNA, Satellite; D005819:Genetic Markers