Synthetic peptides and ribosomal proteins as substrate for 60S ribosomal protein kinase from yeast cells.
Kinetic studies on the 60S protein kinase were conducted with synthetic peptides and ribosomal proteins as substrate. Peptide RRREEESDDD proved to be the best synthetic substrate for this enzyme. The peptide has a sequence of amino acids which most closely resembles the structure of potential phosphorylation sites in natural substrates, i.e., acidic ribosomal proteins. The superiority of certain kinetic parameters for 60S kinase obtained with the native whole 80S ribosomes over those of the isolated fraction of acidic ribosomal proteins indicates that the affinity of 60S kinase to the specific protein substrate not only depends on the structure of the polypeptide chain around the target amino acid but also on its native structure within the 80S ribosome.
Department of Molecular Biology, Maria Curie-Skłodowska University, Lublin, Poland.
N
N
Grankowski
E
E
Gasior
O G
OG
Issinger
D000595:Amino Acid Sequence; D047388:Casein Kinases; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
D010455:Peptides; D012269:Ribosomal Proteins; D011494:Protein Kinases; C083550:60S ribosomal protein kinase; D047388:Casein Kinases; D017346:Protein Serine-Threonine Kinases