Protein secretion by the mouse blastocyst: stimulatory effect on secretion into the blastocoel by transforming growth factor-alpha.
We have previously demonstrated that newly synthesized proteins are secreted into the mouse blastocoel [Dardik and Schultz (1991): Biol Reprod 45:328-333]. In the present study we examine the effect of transforming growth factor-alpha (TGF-alpha) on these proteins. We observe that TGF-alpha stimulates secretion of these newly synthesized proteins into the blastocoel and apical medium, which faces the zona pellucida, by about 65%. Although one-dimensional gel electrophoretic analysis does not reveal any marked differences in the patterns of newly synthesized proteins secreted into the blastocoel in response to TGF-alpha, zymography reveals a marked stimulation in the secretion of several gelatinases into the blastocoel and apical medium. These results suggest additional functions for TGF-alpha in mouse preimplantation development.
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
A
A
Dardik
A S
AS
Doherty
R M
RM
Schultz
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005314:Embryonic and Fetal Development / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology; D018093:Gelatinases; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D010434:Pepsin A / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology* / Q000502:physiology
D011506:Proteins; D016211:Transforming Growth Factor alpha; D010434:Pepsin A; D018093:Gelatinases