Increased extracellular calcium concentrations in the isolated rat heart: effects on mitochondrial contact site formation.
In our previous study, mitochondrial creatine kinase (Mi-CK) activity is localised cytochemically in heart tissue. The activity was found to be exclusively localised in mitochondrial contact sites and the surface density of Mi-CK was increased upon myocardial stimulation (Biermans et al., 1989). As calcium is involved as an intracellular messenger of stimulation, we compared hearts noradrenaline-stimulated in vivo with isolated hearts perfused with buffers containing different calcium concentrations on the surface density Mi-CK.
Bakker
A
A
University of Antwerp (UIA), Dept. of Medicine, Belgium.
De Bie
M
M
Bernaert
I
I
Ravingerova
T
T
Ziegelhöffer
A
A
Van Belle
H
H
Jacob
W
W
D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000494:pharmacology; D003402:Creatine Kinase / Q000032:analysis; D005110:Extracellular Space / Q000737:chemistry*; D005260:Female; D006321:Heart / Q000187:drug effects; D007527:Isoenzymes; D008929:Mitochondria, Heart / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure*; D009124:Muscle Proteins / Q000032:analysis; D009206:Myocardium / Q000737:chemistry*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar / Q000378:metabolism; D015290:Second Messenger Systems
D007527:Isoenzymes; D009124:Muscle Proteins; D003402:Creatine Kinase; D002118:Calcium; D009638:Norepinephrine