Three new small nucleolar RNAs that are psoralen cross-linked in vivo to unique regions of pre-rRNA.
We have recently described three novel human small nucleolar RNA species with unique nucleotide sequences, which were named E1, E2, and E3. The present article describes specific psoralen photocross-linking in whole HeLa cells of E1, E2, and E3 RNAs to nucleolar pre-rRNA. These small RNAs were cross-linked to different sections of pre-rRNA. E1 RNA was cross-linked to two segments of nucleolar pre-rRNA; one was within residues 697 to 1163 of the 5' external transcribed spacer, and the other one was between nucleotides 664 and 1021 of the 18S rRNA sequence. E2 RNA was cross-linked to a region within residues 3282 to 3667 of the 28S rRNA sequence. E3 RNA was cross-linked to a sequence between positions 1021 and 1639 of the 18S rRNA sequence. Primer extension analysis located psoralen adducts in E1, E2, and E3 RNAs that were enriched in high-molecular-weight fractions of nucleolar RNA. Some of these psoralen adducts might be cross-links of E1, E2, and E3 RNAs to large nucleolar RNA. Antisense oligodeoxynucleotide-targeted RNase H digestion of nucleolar extracts revealed accessible segments in these three small RNAs. The accessible regions were within nucleotide positions 106 to 130 of E1 RNA, positions 24 to 48 and 42 to 66 of E2 RNA, and positions 7 to 16 and about 116 to 122 of E3 RNA. Some of the molecules of these small nucleolar RNAs sedimented as if associated with larger structures when both nondenatured RNA and a nucleolar extract were analyzed.
Rimoldi
O J
OJ
Department of Pathology, St. Louis University School of Medicine, Missouri 63104-1028.
Raghu
B
B
Nag
M K
MK
Eliceiri
G L
GL
D001483:Base Sequence; D015152:Blotting, Northern; D003432:Cross-Linking Reagents; D005363:Ficusin; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000737:chemistry*; D012342:RNA, Small Nuclear / Q000737:chemistry*; D015183:Restriction Mapping; D016914:Ribonuclease H
D003432:Cross-Linking Reagents; D012322:RNA Precursors; D012342:RNA, Small Nuclear; D016914:Ribonuclease H; D005363:Ficusin