Improvement of the selectivity of an FIA amperometric biosensor system for glucose.
A flow injection analysis (FIA) biosensor system has been developed for the determination of glucose from urine, blood plasma and foodstuffs. Glucose oxidase was immobilized onto porous aminopropyl glass beads via glutaraldehyde activation to form an enzyme column. The hydrogen peroxide released from the conversion of glucose to gluconic acid was monitored by a platinum electrode vs. silver/silver chloride poised at +700 mV. As a novel aspect to the improvement of the selectivity of the biosensor system, an anion exchange column was placed upstream to remove uric acid, ascorbic acid or acetaminophen, three major electroactive interfering substances which usually occur in urine and blood plasma. Among several resins tested, the effective adsorption of uric and ascorbic acids could be accomplished using an acetate anion exchanger, and the selectivity coefficient was pH dependent. The binding of acetaminophen to the resin was much less efficient and, in all cases, the selectivity coefficient was independent of the operating temperature up to 37 degrees C. When applied to real samples, the data obtained by the biosensor system compared well with those of the standard hexokinase assay. The immobilized glucose oxidase could be reused for at least 2000 repeated analyses without loss of its original activity.
Male
K B
KB
Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec.
Luong
J H
JH
D000082:Acetaminophen / Q000302:isolation & purification; D001205:Ascorbic Acid / Q000302:isolation & purification; D001786:Blood Glucose / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis; D005504:Food Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006029:Glycosuria / Q000378:metabolism*; D006801:Humans; D012680:Sensitivity and Specificity; D014527:Uric Acid / Q000302:isolation & purification
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D014527:Uric Acid; D000082:Acetaminophen; D005949:Glucose Oxidase; D005947:Glucose; D001205:Ascorbic Acid