Tyrphostin-induced differentiation of mouse erythroleukemia cells.
Inhibitors of protein-tyrosine kinases (TPKs) from the tyrphostins family induce terminal erythroid differentiation of mouse erythroleukemia (MEL) cells. The most potent tyrphostin was found to be AG-568 which was therefore investigated in more detail. Just prior to differentiation the inhibition of tyrosine phosphorylation of a pp97 protein band was noted. We also found that AG-568 treatment induces the appearance of a putative differentiation factor which could induce tyrphostin-independent differentiation in untreated cells. Our study suggests that the inhibition of tyrosine phosphorylation by AG-568 leads to the production of differentiating factor(s) which induce the MEL cells to differentiate.
Lautenberg Center for Immunology, Hebrew University Hadassah Medical School, Jerusalem, Israel.
M
M
Anafi
A
A
Gazit
C
C
Gilon
Y B
YB
Neriah
A
A
Levitzki
D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D003470:Culture Media; D004920:Erythropoiesis / Q000187:drug effects*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D009570:Nitriles / Q000494:pharmacology*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism; D020032:Tyrphostins
D002396:Catechols; D003470:Culture Media; D009570:Nitriles; D020032:Tyrphostins; C060152:tyrphostin 47; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases