Allergen-induced airway hyperresponsiveness in Brown-Norway rat: role of parasympathetic mechanisms.
Enhanced parasympathetic mechanisms may contribute to airway hyperresponsiveness. The present study examined whether the in vivo increase in airway responsiveness seen 18-24 h after either a single or chronic aerosolized allergen challenge protocol in actively sensitized Brown-Norway rats was due to altered parasympathetic mechanisms. The roles of central and reflex vagal mechanisms were studied by performing bilateral cervical vagotomy before measurement of airway responsiveness. Bilateral vagotomy failed to reduce the increase in airway responsiveness after either a single or chronic allergen challenge. The roles of increased neural release of acetylcholine (ACh) and increased end organ responsiveness were studied in vitro. The isometric responses of tracheal and bronchial strips to both electrical field stimulation and exogenously applied ACh from rats exposed both to single and chronic allergen challenges were compared with those from saline-exposed rats. The responses to electrical field stimulation and to exogenous ACh were not significantly enhanced 18-24 h after either protocol. We conclude that the airway hyperresponsiveness observed in this allergic rat model is not mediated through an enhancement of parasympathetic mechanisms.
Department of Thoracic Medicine, National Heart and Lung Institute, London, United Kingdom.
W
W
Elwood
T
T
Sakamoto
P J
PJ
Barnes
K F
KF
Chung
D000109:Acetylcholine / Q000378:metabolism / Q000494:pharmacology; D000485:Allergens / Q000494:pharmacology*; D000818:Animals; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism; D016535:Bronchial Hyperreactivity / Q000503:physiopathology*; D004558:Electric Stimulation; D008168:Lung / Q000503:physiopathology; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects / Q000294:innervation / Q000503:physiopathology; D010047:Ovalbumin / Q000276:immunology; D010275:Parasympathetic Nervous System / Q000503:physiopathology*; D051381:Rats; D011914:Rats, Inbred BN; D012018:Reflex / Q000502:physiology; D012129:Respiratory Function Tests; D014132:Trachea / Q000187:drug effects / Q000378:metabolism; D014628:Vagotomy
D000485:Allergens; D010047:Ovalbumin; D000109:Acetylcholine