Glutathione, but not glutamine, is detected in 13C-NMR spectra of perchloric acid extracts from C6 glioma cells.
Glutamine, which is expected to be produced by C6 glioma cells, is not detected in both amino-acid analyses and 13C-NMR spectra of perchloric acid extracts of cells incubated for 4 h with [1-13C]glucose in the absence of extracellular glutamine. However, the resonances of a glutamate-linked product are observed in these spectra. The analysis of the pH dependence of chemical shifts from various glutamate-derived compounds shows that the observed resonances came from glutathione. Glutamine and glutathione signals are in close proximity on the frequency scale, leading to possible misinterpretation of the spectra.
Portais
J C
JC
Département de Résonance Magnétique, CNRS et Université de Bordeaux II, France.
Martin
M
M
Canioni
P
P
Merle
M
M
D001932:Brain Neoplasms / Q000737:chemistry*; D002247:Carbon Isotopes; D005910:Glioma / Q000737:chemistry*; D005973:Glutamine / Q000032:analysis*; D005978:Glutathione / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010472:Perchlorates / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D002247:Carbon Isotopes; D010472:Perchlorates; D005973:Glutamine; D005978:Glutathione