Phosphocreatine T1 measurements with and without exchange in the heart.
The intrinsic phosphocreatine (PCr) T1 values measured by time-dependent magnetization transfer in isolated perfused rat, hamster, and turkey hearts were indistinguishable. The value of 3.5 +/- 0.3 s for the rat heart is similar to values measured by other magnetization transfer methods. Irreversibly inhibiting the phosphoryl exchange between PCr and ATP in the rat heart using iodoacetamide changed the apparent T1 values of the two exchanging species when measured by inversion recovery: The apparent T1 of PCr increased from 1.92 +/- 0.06 s to 3.55 +/- 0.06 s, in excellent agreement with the intrinsic T1 measured by magnetization transfer. The apparent T1 of [gamma-P]ATP decreased from 0.92 +/- 0.07 s to 0.44 +/- 0.03 s. The value for the T1 of [gamma-P]ATP in hearts with inhibited phosphoryl exchange was similar to T1 values for [alpha-P]ATP and [beta-P]ATP, which remained unchanged. This illustrates that apparent T1 values for PCr and [gamma-P]ATP measured by inversion recovery in the presence of exchange are average T1 values in between the intrinsic values. The large differences between the intrinsic T1 measured by magnetization transfer and the T1 measured by inversion recovery makes the use of the appropriate value in different applications quantitatively important.
Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115.
J
J
Friedrich
L
L
Nascimben
R
R
Liao
J S
JS
Ingwall
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D003402:Creatine Kinase / Q000037:antagonists & inhibitors; D006224:Cricetinae; D007460:Iodoacetamide / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008647:Mesocricetus; D008954:Models, Biological; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D010710:Phosphates / Q000032:analysis; D010725:Phosphocreatine / Q000032:analysis*; D010758:Phosphorus; D051381:Rats; D017207:Rats, Sprague-Dawley; D014422:Turkeys
D010710:Phosphates; D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D007460:Iodoacetamide