Comparison of the action of two protein kinase C activators on dihydropyridine-sensitive Ca2+ channels in neonatal rat ventricular myocytes.
Ca2+ channels can be modulated by protein kinase C which phosphorylates Ca2+ channel proteins. Two protein kinase C activators, 4 beta-phorbol 12-myristate 13-acetate (PMA) and 1-oleyl-2-acetyl-rac-glycerol (OAG), were used to investigate the effect of protein kinase C activation on dihydropyridine-sensitive Ca2+ channel currents (L-type) in neonatal rat ventricular myocytes using the whole cell version of the patch clamp technique. The results show that both of the activators increased the Ca2+ channel current in myocytes. However, these increases in Ca2+ channel current were different as function of time. Therefore, our results suggest that the temporal factor should be considered when the protein kinase C activators are used in studies of channel modulation.
Department of Physiology, University of Alberta, Edmonton, Canada.
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D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000187:drug effects*; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects; D009543:Nifedipine / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015220:Calcium Channels; D004075:Diglycerides; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C; D009543:Nifedipine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium