Primary structures and expression from cDNAs of rat opioid receptor delta- and mu-subtypes.
The complete amino acid sequences of rat opioid receptors (designated as ROR-A and ROR-B) have been deduced by cloning and sequencing the cDNAs. The ligand-binding properties of ROR-A and ROR-B expressed from the cloned cDNAs in Chinese hamster ovary cells correspond most closely to those of the pharmacologically defined delta- and mu-opioid receptor subtypes, respectively. RNA blot hybridization analysis revealed that cerebrum and brainstem contain both ROR-A and ROR-B mRNAs, whereas neither ROR-A nor ROR-B mRNAs can be detected in cerebellum.
Department of Anesthesia, Kyoto University Hospital, Japan.
K
K
Fukuda
S
S
Kato
K
K
Mori
M
M
Nishi
H
H
Takeshima
D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004247:DNA; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D017465:Receptors, Opioid, delta / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D016415:Sequence Alignment
D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D004247:DNA