Determination of safrole, dihydrosafrole, and chloromethyldihydrosafrole in piperonyl butoxide by high-performance liquid chromatography.
An HPLC method for the simultaneous determination of safrole (S), dihydrosafrole (DHS), and chloromethyldihydrosafrole (CM-DHS) in piperonyl butoxide, with fluorimetric detection and elution gradient, is described. Samples with internal standard (piperonyl isobutyrate) are adsorbed on silica cartridges, then eluted with an appropriate solvent. Internal standard, S, DHS, and CMDHS are eluted together and injected into a reversed-phase chromatography column. The concentrations of the three products are calculated from calibration graphs, and linearity and reproducibility are verified. The limit of detection is 2 mg.kg-1. This analytical method allows for control of the synthesis process of piperonyl butoxide and determination of some carcinogenic substances, such as S and DHS.
Laboratory of Analytical Chemistry, UFR de Pharmacie, Marseille, France.
E M
EM
Schreiber-Deturmeny
A M
AM
Pauli
J L
JL
Pastor
D002851:Chromatography, High Pressure Liquid; D010882:Piperonyl Butoxide / Q000032:analysis*; D012451:Safrole / Q000031:analogs & derivatives* / Q000032:analysis*
C083264:5-chloromethyl-6-propyl-1,3-benzodioxole; C035396:dihydrosafrole; D010882:Piperonyl Butoxide; D012451:Safrole