Meningocortical lesion increases expression of the cholecystokinin gene in rat cerebral cortex: evidence for the involvement of platelet-activating factor (PAF).
In rat neocortex, interneurons express the gene encoding cholecystokinin. After an injury to the meninges and the underlying cortex the levels of cholecystokinin mRNA are transiently enhanced in the ipsilateral hemisphere. In the present study, we have investigated, whether platelet-activating factor plays a role in this phenomenon. Two antagonists of platelet-activating receptors, i.e. WEB 2086 (1.5 mg/kg) and brotizolam (10 mg/kg), were used. When injected 30 min prior to the injury of the parietal cortex, both agents reduced the rise in the concentration of cholecystokinin mRNA in frontal cortex by approximately 60%. They had no significant effect when given 30 min after the injury. Our finding that antagonists of platelet-activating factor receptors diminish the injury-induced change in the activity of cholecystokinin-interneurons opens the possibility that these agents may also affect other pathophysiological aspects of brain trauma.
Department of Pharmacology, University of Freiburg, FRG.
E
E
Götz
C
C
Olenik
A
A
Uhl
A
A
Seregi
D K
DK
Meyer
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002540:Cerebral Cortex / Q000293:injuries* / Q000378:metabolism*; D002766:Cholecystokinin / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D008084:Lipoxygenase / Q000378:metabolism*; D008297:Male; D008578:Meninges / Q000293:injuries*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D010980:Platelet Membrane Glycoproteins; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors; D043562:Receptors, G-Protein-Coupled; D014230:Triazoles / Q000494:pharmacology
D001381:Azepines; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D014230:Triazoles; C039351:platelet activating factor receptor; C052518:WEB 2086; C020960:brotizolam; D002766:Cholecystokinin; D008084:Lipoxygenase