1H editing of 2-deuterated exogenous and natural endogenous D-glucose in biological samples.
A spin-echo based 1H homonuclear pulse sequence, enabling the selective editing of homonuclear first-order J-multiplet types, is presented and analyzed. Its effectiveness in quantification applications is assessed. Its potential usefulness in the quantitative distinction between 2-deuterated and natural D-glucose in biological samples is briefly discussed, with emphasis on studies of hexose metabolism conducted in vitro.
VUB, High Resolution NMR Centre, Belgium.
M
M
Biesemans
F
F
Kayser
M
M
Van Cauteren
N J
NJ
Rehrer
P
P
Neirinck
K
K
de Meirleir
W J
WJ
Malaisse
R
R
Willem
D000042:Absorption; D001786:Blood Glucose / Q000032:analysis*; D003903:Deuterium; D005947:Glucose / Q000032:analysis*; D006801:Humans; D006859:Hydrogen; D007089:Image Enhancement / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013997:Time Factors
D001786:Blood Glucose; D006859:Hydrogen; D003903:Deuterium; D005947:Glucose