Isolation of seven respiratory viruses in shell vials: a practical and highly sensitive method.
The isolation of respiratory viruses in shell vials was compared with isolation in tube cultures in order to determine the sensitivity of the former, rapid method. Twenty of 21 influenza virus and 15 of 15 parainfluenza virus isolates were recovered in shell vials. One hundred twenty-seven of 138 respiratory syncytial virus isolates were detected in shell vials, but only 10 of 21 adenovirus isolates were positive by the rapid method. Shell vials are very effective for the diagnosis of respiratory viral infections, except for those caused by adenovirus.
Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178.
M A
MA
Olsen
K M
KM
Shuck
A R
AR
Sambol
S M
SM
Flor
J
J
O'Brien
B J
BJ
Cabrera
D000260:Adenoviruses, Human / Q000302:isolation & purification; D000328:Adult; D002648:Child; D005069:Evaluation Studies as Topic; D006801:Humans; D007223:Infant; D009975:Orthomyxoviridae / Q000302:isolation & purification; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification; D012137:Respiratory System / Q000382:microbiology*; D012141:Respiratory Tract Infections / Q000175:diagnosis; D018108:Respirovirus / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data; D014777:Virus Diseases / Q000175:diagnosis; D014780:Viruses / Q000302:isolation & purification*