Sequence variability of the glycoprotein gene of bovine respiratory syncytial virus.
Sequence variation in the attachment glycoprotein G of bovine respiratory syncytial virus (BRSV) was determined. The nucleotide sequences of the G mRNAs of the A51908, VC464 and FS-1 strains of BRSV were compared with the published sequence of the BRSV strain 391-2. Nucleotide sequence alignment showed that overall they are highly conserved, with 90 to 97% identity. In addition, the coding region of strain A51908 was longer by 18 nucleotides at the 3' end. An 84 to 95% level of identity was observed among the deduced amino acid sequences of the G proteins of BRSV strains. A maximum divergence of 19% was found when the extracellular domains of the G proteins were compared. The level of diversity would be consistent, by analogy to the human respiratory syncytial viruses, with these BRSV strains forming a single subgroup.
Mallipeddi
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VA-MD Regional College of Veterinary Medicine, University of Maryland, College Park 20742.
Samal
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SK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005814:Genes, Viral; D014644:Genetic Variation; D015707:HN Protein; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012136:Respiratory Syncytial Viruses / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D015707:HN Protein; D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G