Cyclic adenosine 3',5'-monophosphate, a second messenger in interleukin-1 mediated K562 cytostasis.
This study evaluates intracellular cAMP concentrations, in interleukin-1 (IL-1) mediated cytostasis of K562 cells. Dibutyryl-cAMP, forskolin, staurosporine and phorbol 12-myristate 13-acetate were all found to mimic the cytostatic action of IL-1 on K562 cells. IL-1 was found to elicit a dose-dependent rise in adenyl cyclase activity in isolated K562 membranes, as well as increasing cAMP concentrations in whole cells. cAMP concentrations were found to peak within 3 minutes of IL-1 stimulation. A 27-29% reduction in proliferative activity elicited by IL-1, forskolin and staurosporine, correlated with a 6 fold increase in adenyl cyclase activity, in each case.
Department of Medical Biochemistry, University of the Witwatersrand Medical School, Johannesburg, South Africa.
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Weitzmann
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Savage
D000262:Adenylyl Cyclases / Q000378:metabolism; D000470:Alkaloids / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D015290:Second Messenger Systems; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D007375:Interleukin-1; D005576:Colforsin; D003994:Bucladesine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate