Excitatory effect of propentofylline on neurotransmission in guinea pig hippocampal slice.
The postsynaptic field potential (population spike; PS) was recorded from the granule cell layer of guinea pig hippocampal slices after electrical stimulation of the perforant path. Propentofylline, a xanthine derivative, enhanced the amplitude of PS to 120-150% of the original level at a concentration range of 100 nM to 1 mM. Theophylline at concentrations over 10 microM increased the PS amplitude to 200% of the original amplitude. Simultaneous application of 1-(5-isoquin-olinesulfonyl)-2-methylpiperazine dihydrochloride (H-7; 100 microM), a protein kinase C (PKC) inhibitor, significantly blocked the excitatory effect of propentofylline, but it failed to counteract the excitatory effect of theophylline. These results indicate that the excitatory effect of propentofylline is mediated by a metabolic process involving PKC and that the protective effect of propentofylline on neuronal damage caused by hypoxia is not due to the inhibition of synaptic transmission.
Kimura
Y
Y
Department of Physiology, School of Medicine, Kobe University, Japan.
Yamada
Y
Y
Okada
Y
Y
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects*; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D010477:Perfusion; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D013569:Synapses / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects*; D013806:Theophylline / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology*
D000897:Anti-Ulcer Agents; D007546:Isoquinolines; D010879:Piperazines; D014970:Xanthines; C032114:propentofylline; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D013806:Theophylline; D011493:Protein Kinase C