Persistence of intestinal antibody response to heterologous rotavirus infection in a murine model beyond 1 year.
We used an ELISPOT (enzyme-linked immunosorbent spot) assay to quantitate the long-term rotavirus-specific intestinal antibody response in a murine model. The frequency of murine intestinal antibody-secreting cells (ASCs) was followed for a period of 1 year after a single dose of rhesus rotavirus (10(6) PFU) was administered at 10 days of age. Some animals were boosted at that time with a second dose. One year after infection, virus-specific ASCs declined from acute-phase levels, but they were still present at significant levels (1.32 x 10(4) virus-specific ASCs per 10(6) intestinal mononuclear cells; approximately 17% of the previously reported response at 1 month after infection). A booster dose 1 year after the primary infection produced a 100% increase in virus-specific ASCs but did not restore the response to that of the primary infection.
Shaw
R D
RD
Department of Medicine, Northport Veterans Affairs Medical Center, New York 11768.
Merchant
A A
AA
Groene
W S
WS
Cheng
E H
EH
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007071:Immunoglobulin A, Secretory / Q000032:analysis / Q000096:biosynthesis; D007421:Intestine, Small / Q000276:immunology; D051379:Mice; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology*; D013997:Time Factors
D000914:Antibodies, Viral; D007071:Immunoglobulin A, Secretory