[Preparing monoclonal antibodies to thyroxine].
Spleen cells of BALB/c mouse immunized with the keyhole limpet haemocyanin and thyroxine conjugate were fused with P3-X63-Ag8.653 mouse myeloma cells to produce. Four monoclonal antibodies selected by indirect ELISA and partially characterized. One antibody, 1B7, chosen for immunoassay, was produced in mouse ascites fluid, purified and analyzed; it proved to belong to the IgG1 subclass. The cross-reactivity with triiodothyronine was less than 1%, the association constant was 3 x 10(9) M-1.
N D
ND
Danilova
N V
NV
Krupina
M V
MV
Mertts
R G
RG
Vasilov
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000008:administration & dosage; D006460:Hemolysin Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013974:Thyroxine / Q000008:administration & dosage / Q000276:immunology*; D014284:Triiodothyronine / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000941:Antigens; D006460:Hemolysin Proteins; D014284:Triiodothyronine; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D013974:Thyroxine