Synthesis and properties of the peptide corresponding to the ACTH-like sequence of human immunoglobulin G1.
The decapeptide Val-Lys-Lys-Pro-Gly--Ser-Ser-Val-Lys-Val (termed immunocorticotropin) corresponding to the sequence 11-20 of the variable part of the human immunoglobulin G1 heavy chain was synthesized. The ACTH-like peptide was found to have an ability to increase body temperature. Intracerebroventricular injection of 5-10 micrograms of the peptide to rabbits induced an elevation of body temperature by 0.7-1.3 degrees C. The ACTH-like peptide was shown to compete with [125I]-ACTH (13-24) for binding to receptors on murine brain synaptic membranes and to activate adenylate cyclase of these membranes.
Mitin
Y V
YV
Institute of Immunology, Biopreparation State Concern, Moscow Region, Russia.
Navolotskaya
E V
EV
Vasilenko
R N
RN
Abramov
V M
VM
Zav'Yalov
V P
VP
D000262:Adenylyl Cyclases / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D011817:Rabbits; D018043:Receptors, Corticotropin; D011979:Receptors, Pituitary Hormone / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism
C082552:ACTH (13-24); D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D010446:Peptide Fragments; D018043:Receptors, Corticotropin; D011979:Receptors, Pituitary Hormone; C082551:immunocorticotropin; D000324:Adrenocorticotropic Hormone; D000262:Adenylyl Cyclases