Function of gene 49 of bacteriophage T4 III. Isolation of Holliday structures from very fast-sedimenting DNA.
Branched DNA molecules were identified in ClaI digests of cytosine containing very fast-sedimenting DNA (VFS-DNACYT) which was isolated from Escherichia coli infected with the multiple mutant 49-GT7 of phage T4. In about 10% of randomly picked ClaI fragments branches with three arms (Y-structures) as well as four arms (Holliday structures) were seen in the electron microscope. Branched structures were absent from ClaI digests after treatment with purified endonuclease VII (gp49) in vitro.
Institute for Genetics, Köln, Germany.
M
M
Flemming
B
B
Deumling
B
B
Kemper
D017122:Bacteriophage T4 / Q000235:genetics*; D004279:DNA, Viral / Q000378:metabolism / Q000648:ultrastructure*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D009690:Nucleic Acid Conformation
D004279:DNA, Viral; C032824:ATCGAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific