The expression of H-like blood group glycolipids in small cell carcinoma of the lung.
Monoclonal antibody SM1 has been shown to be preferentially reactive with small cell carcinoma of the lung (SCCL) cell lines by fluorescent and radioimmunoassay membrane staining (1). Using solid phase indirect radioimmunoassay, the antigen is not detected in non-SCCL lung carcinomas histologically classified as squamous carcinoma, adenocarcinoma or large cell carcinoma, and other tumors, viz; pheochromocytoma, a mesoderm derived lymphoblastic leukemia cell line or in normal human brain, heart, liver, colon, endothelial tissues of the aorta and blood vessels, skin, omentum, muscle, lung parenchyma and is weakly reactive with bronchial mucosa, pancreas, and kidney. The membrane antigens detected by SM1 were isolated from small cell carcinoma of the lung (SCCL) cell line, SW2, using anion exchange chromatography and thin layer chromatography, and were further analysed by exoglycosidase and endoglycosidase treatments followed by chemical staining and immunostaining with SM1 and other antibodies. We show here that SM1 antibody reacts with a group of fucose-containing neutral glycolipids and gangliosides many of which are cross-reactive with antibodies to H antigens.
Cualing
H
H
Department of Pathology, University of Cincinnati School of Medicine, OH 45267.
Siegel
R
R
Schwarting
G A
GA
Suchy
S F
SF
McCluer
R H
RH
Bernal
S
S
D000017:ABO Blood-Group System / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D018288:Carcinoma, Small Cell / Q000276:immunology / Q000378:metabolism*; D003429:Cross Reactions; D005732:Gangliosides / Q000276:immunology; D006017:Glycolipids / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D006028:Glycosphingolipids / Q000276:immunology; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology / Q000378:metabolism*; D005644:alpha-L-Fucosidase / Q000378:metabolism
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D005732:Gangliosides; D006017:Glycolipids; D006028:Glycosphingolipids; D005644:alpha-L-Fucosidase