Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments.
DNA of the variola major virus strain India-1967 in the region of HindIII M, L, I fragments has been sequenced. Analysis of this sequence of 18029 bp revealed 19 potential open reading frames (ORFs). Four proposed proteins (L2R, H9R, L5L, L6R) contain metal-binding domains. Comparison of the variola virus (VAR) and vaccinia virus strain Copenhagen (COP) sequences show that the main differences are between proteins L1R and I5R. L1R contains 6 additional amino acid residues on the C-terminus. The protein I5R of VAR contains three Ca2+ binding domains but this COP has deletions in 2 of the 3 established domains. Possible functions of the predicted viral polypeptides are discussed.
Shchelkunov
S N
SN
Institute of Molecular Biology, NPO Vector, Koltsovo, Novosibirsk region, Russia.
Blinov
V M
VM
Totmenin
A V
AV
Marennikova
S S
SS
Kolykhalov
A A
AA
Frolov
I V
IV
Chizhikov
V E
VE
Gytorov
V V
VV
Gashikov
P V
PV
Belanov
E F
EF
D000595:Amino Acid Sequence; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011487:Protein Conformation; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014616:Vaccinia virus / Q000235:genetics; D012901:Variola virus / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D004279:DNA, Viral; D008670:Metals; D014764:Viral Proteins