Simultaneous determination of 6-monoacetylmorphine, morphine and codeine in urine using high-performance liquid chromatography with combined ultraviolet and electrochemical detection.
A method is described for the simultaneous determination of 6-monoacetylmorphine (6-MAM), morphine and codeine in post-mortem urine specimens using reversed-phase high-performance liquid chromatography with dual ultraviolet spectrophotometric and electrochemical detection. The limits of detection for a 1-ml urine sample were 0.04 mg/l for 6-MAM and 0.05 mg/l for both morphine and codeine. The presence of 6-MAM in urine indicates prior use of heroin and enables differentiation between morphine- and heroin-related deaths.
Victorian Institute of Forensic Pathology, Monash University, South Melbourne, Australia.
J
J
Gerostamoulos
K
K
Crump
I M
IM
McIntyre
O H
OH
Drummer
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000652:urine*; D004563:Electrochemistry; D006801:Humans; D009020:Morphine / Q000652:urine*; D009022:Morphine Derivatives / Q000652:urine*; D011180:Postmortem Changes; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D009022:Morphine Derivatives; D009020:Morphine; C026979:6-O-monoacetylmorphine; D003061:Codeine