Resistance of genetically selected mice to MHV3 infection is not dependent on the H2O2 release by macrophages.
The genetically selected high antibody responder line of mice (HIII) for a natural immunogen were fully susceptible to mouse hepatitis virus 3 (MHV3) and the corresponding low antibody responder mice (LIII) were fully resistant, regardless of whether they were previously treated or not with the bacillus Calmette-Guérin (BCG). The resistance or susceptibility correlated with the virus growth in the peritoneum of mice. Peritoneal cells isolated from resistant mice released higher amounts of H2O2 after phorbol myristate acetate (PMA) stimulation than susceptible mice. No spontaneous in vitro H2O2 release by peritoneal exudate cells from HIII mice, shown to be mostly macrophages, were observed during the MHV3 infection. In contrast, the spontaneous in vitro H2O2 release by these cells from MHV3-infected LIII mice increased gradually, reaching a maximal value 3 days after infection, and decreased in parallel to the virus clearance from the peritoneum. The BCG treatment primed the macrophages of HIII mice for the production of H2O2 during the MHV3 infection, but did not confer resistance against the virus infection. The data obtained suggest that the acquired capability of macrophages to release H2O2 does not participate in the anti-MHV3 activity or resistance against the MHV3 infection.
Instituto Butantan, Laboratorio de Imunologia Viral, São Paulo, Brasil.
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D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D001202:Ascitic Fluid / Q000382:microbiology; D001500:BCG Vaccine / Q000276:immunology; D006524:Hepatitis, Viral, Animal / Q000276:immunology*; D006861:Hydrogen Peroxide; D007113:Immunity, Innate / Q000235:genetics; D008099:Liver / Q000382:microbiology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics / Q000276:immunology*; D006517:Murine hepatitis virus / Q000302:isolation & purification / Q000502:physiology; D012641:Selection, Genetic; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014779:Virus Replication
D000914:Antibodies, Viral; D001500:BCG Vaccine; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate