Partial purification of paraoxonase from rat liver.
A method for the partial purification of rat liver paraoxonase is presented. The method consists of the following steps: preparation of microsomes, solubilization with Triton X-100, adsorption on hydroxylapatite and chromatography on DEAE-52 cellulose. A partially purified preparation of rat liver paraoxonase has been obtained, showing a specific activity of 422 mU/mg with a yield of about 22% and a purification factor of 77-fold.
Department of Legal Medicine and Toxicology Service, Faculty of Medicine, University Hospital, University of Granada, Spain.
F
F
Gil
A
A
Pla
M C
MC
Gonzalvo
A F
AF
Hernández
E
E
Villanueva
D000818:Animals; D043303:Aryldialkylphosphatase; D008099:Liver / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification; D008862:Microsomes, Liver / Q000201:enzymology; D010744:Phosphoric Monoester Hydrolases / Q000097:blood / Q000302:isolation & purification*; D051381:Rats; D017208:Rats, Wistar
D008565:Membrane Proteins; D010744:Phosphoric Monoester Hydrolases; D043303:Aryldialkylphosphatase