Catalepsy induced by muscimol infused into the hypothalamus can be sensitized and is modulated by ovarian steroids.
Muscimol (25 ng), but not saline, infused into the region of the zona incerta and lateral hypothalamus induced significant catalepsy in female rats. Daily administration of muscimol for 5 days resulted in a sensitization of the cataleptic response such that there was a significantly greater response to the same dose of muscimol beginning on Day 3 and continuing to increase up to Day 5. It was determined that the increase in catalepsy across days was not the result of conditioning after an initial exposure to muscimol. The endocrine condition of the female affected the degree of catalepsy induced by the first exposure to intrahypothalamic muscimol. Ovariectomized rats pretreated with estradiol benzoate (EB) exhibited significantly greater catalepsy than did females untreated or treated with progesterone or combined EB and progesterone. Weekly administration of muscimol also produced significant sensitization of the behavioral response, and the degree of sensitization was again affected by endocrine condition. Specifically, although females treated with EB for 2 days before muscimol infusion showed a significantly greater catalepsy after the first infusion than did ovariectomized females, they failed to show any increase in catalepsy scores across the 4 weeks of treatment. The greater catalepsy induced by muscimol infusion in EB-treated females may be related to changes in dopaminergic transmission. In contrast, females pretreated with progesterone 4 hr before muscimol infusion (with or without prior EB) exhibited highly significant increases in cataleptic response across the 4 weeks.(ABSTRACT TRUNCATED AT 250 WORDS)
Laboratory of Neurobiology and Behavior, Rockefeller University.
M M
MM
McCarthy
L C
LC
Kaufman
D W
DW
Pfaff
S
S
Schwartz-Giblin
D000818:Animals; D001931:Brain Mapping; D002375:Catalepsy / Q000139:chemically induced* / Q000503:physiopathology; D004298:Dopamine / Q000502:physiology; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D007026:Hypothalamic Area, Lateral / Q000187:drug effects* / Q000503:physiopathology; D009118:Muscimol / Q000494:pharmacology*; D010052:Ovariectomy; D010053:Ovary / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D009118:Muscimol; D004958:Estradiol; D005680:gamma-Aminobutyric Acid; C010452:estradiol-17 beta-benzoate; D004298:Dopamine