[Intragenic variable number tandom repeats in coagulation factor VIII gene of Chinese origin].
Using polymerase chain reaction, we analysed the variable number tandom repeats (VNTR) in intron 13 of coagulation factor VIII gene in the Chinese. Study of 115 X chromosomes from 77 normal Chinese revealed that the VNTR is composed of at least 4 different alleles in the Chinese, i.e. 19, 20, 21, and 23 repeats 53% of the females analysed were heterozygous of this VNTR. The discovery and application of this new molecular genetic marker is of significance in genetic consultation and prenatal diagnosis of hemophilia A.
Thrombosis and Haemostasis Research Unit, Jiangsu Institute of Hematology, Suzhou Medical College.
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Wang
D000483:Alleles; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005169:Factor VIII / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D005819:Genetic Markers; D014644:Genetic Variation; D006467:Hemophilia A / Q000235:genetics*; D006801:Humans; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D014960:X Chromosome
D005819:Genetic Markers; D005169:Factor VIII; D004247:DNA