[New site site-specific endonuclease and methylase from Bacillus licheniformis 736].
The site-specific endonuclease R Bli736I and methylase M Bli736I have been isolated from the Bacillus licheniformis strain 736 by blue-agarose, hydroxyapatite-Ultragel and heparin-Sepharose chromatography. The enzymes are free from interfering impurities. R Bli736I recognizes the 5'-GGTCTCN-3' decreases and decreases 5'-NNNNNGAGACC-3' sequences on the DNA and cleaves the DNA as indicated by arrows to form single-stranded 4-nucleotide 5'-protruding termini. This enzyme is an isoschizomer of Eco3II isolated from E. coli.
N N
NN
Matvienko
V M
VM
Kramarov
L A
LA
Zheleznaia
N I
NI
Matvienko
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002850:Chromatography, Gel; D004279:DNA, Viral / Q000378:metabolism; D015257:DNA-Cytosine Methylases / Q000302:isolation & purification* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
D004279:DNA, Viral; C077514:DNA modification methylase Eco31I; D015257:DNA-Cytosine Methylases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C047595:endodeoxyribonuclease Eco31I; D015252:Deoxyribonucleases, Type II Site-Specific