Expression of types I, III and IV collagen genes in fibrotic skin and nerve lesions of toxic oil syndrome patients.
We have studied the skin and nerve fibrosis in toxic oil syndrome by in situ hybridization using specific cDNA probes for types I, III, and IV collagens. Fibroblasts with high levels of type I and III collagen mRNA were observed in biopsies from fibrotic skin areas. Similarly, type IV collagen mRNA was abundant in cells within the fibrotic process of the nerves. These results suggest that the excessive accumulation of collagen in toxic oil syndrome results from transcriptional activation of collagen genes in a subpopulation of fibroblasts.
Rheumatology Unit, Hospital 12 de Octubre, Madrid, Spain.
J J
JJ
Gomez-Reino
M
M
Sandberg
P E
PE
Carreira
E
E
Vuorio
D001345:Autoradiography; D001706:Biopsy; D001937:Brassica; D003094:Collagen / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D005229:Fatty Acids, Monounsaturated; D005355:Fibrosis; D005786:Gene Expression Regulation; D006801:Humans; D017403:In Situ Hybridization; D010523:Peripheral Nervous System Diseases / Q000378:metabolism* / Q000473:pathology; D010938:Plant Oils / Q000506:poisoning*; D012333:RNA, Messenger / Q000378:metabolism; D000074262:Rapeseed Oil; D012595:Scleroderma, Systemic / Q000378:metabolism* / Q000473:pathology; D013497:Sural Nerve / Q000378:metabolism / Q000473:pathology; D013577:Syndrome
D015342:DNA Probes; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D012333:RNA, Messenger; D000074262:Rapeseed Oil; D003094:Collagen