Characterization of human rotavirus strains causing gastroenteritis in Kenya.
Human rotavirus strains from Kenya, from children with gastroenteritis in an urban area (Nairobi) and three rural areas were characterized by antigenic and genomic analysis. While in all areas strains with subgroups II and G serotype 1 antigens were most common, two unusual strains were detected. One strain (NK59: subgroup II, G serotype 4) possessed an additional RNA band on polyacrylamide gel electrophoresis, the other (D202) which had antigenic specificity of subgroup II and G serotype 1 showed a 'short' RNA pattern. The latter strain was adapted to growth in cell culture.
Gatheru
Z
Z
Virus Research Centre, Kenya Medical Research Institute, Nairobi.
Kobayashi
N
N
Adachi
N
N
Chiba
S
S
Muli
J
J
Ogaja
P
P
Nyangao
J
J
Kiplagat
E
E
Tukei
P M
PM
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000382:microbiology*; D005814:Genes, Viral / Q000235:genetics; D005838:Genotype; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D012367:RNA, Viral