Over-expression of p53 protein in human laryngeal carcinoma.
We examined the expression of tumor-suppressor protein p53 in a variety of laryngeal carcinomas from 43 patients (25 primary, 13 metastatic and 5 recurrent cases), 13 normal laryngeal tissues and 7 benign laryngeal nodule biopsy specimens, using the polyclonal antibody CM-1. Previously we have reported a high incidence of ras mutations (51%) and human papillomavirus (HPV) infection (37%) in these laryngeal carcinomas. p53 protein was detected by immunohistochemistry in 65% of laryngeal carcinomas (60% of primary, 69% of metastatic and 80% of recurrent cases). No correlation was found between p53 over-expression and histological grading of the tumors. None of the specimens from normal larynx and laryngeal nodules revealed any detectable level of this protein. Furthermore, 11 (69%) of 16 HPV-positive cases and 17 (77%) of 22 cases with ras mutation showed variable grades of p53 expression. Twelve (71%) of 17 laryngeal carcinomas in current study having both p53 over-expression and ras mutation were moderately or poorly differentiated. Likewise, positivity for these 2 parameters was significantly increased in metastatic tumors (9 of 13 cases, 69%) as compared with primary and recurrent tumors (8 of 30 cases, 27%) (p < 0.01). Our results suggest that multiple factors are involved in this malignancy, and that the simultaneous over-expression of p53 and the presence of ras mutation may be related to the progression stage of laryngeal carcinoma.
Department of Pathology, Fukui Medical School, Japan.
K
K
Anwar
K
K
Nakakuki
H
H
Imai
H
H
Naiki
M
M
Inuzuka
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007818:Laryngeal Diseases / Q000235:genetics / Q000276:immunology; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000382:microbiology; D007830:Larynx / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D000906:Antibodies; D004279:DNA, Viral; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D016159:Tumor Suppressor Protein p53