Rapid preparation of subsarcolemmal and interfibrillar mitochondrial subpopulations from cardiac muscle.
1. Subsarcolemmal and interfibrillar mitochondria were prepared with complete recovery from rabbit and porcine heart muscle by upward-flotation during 60 sec of Percoll density gradient centrifugation. 2. Mitochondrial subpopulations were identified and characterized according to buoyant density, electron-microscopy, marker enzyme activities and respiratory performance. 3. ADP-induced state 3-respiration related to latent citrate synthase activity as a marker for structurally intact mitochondria was not significantly different in both mitochondrial subtypes.
Medizinische Klinik, Georg-August-Universität, Göttingen, Germany.
J M
JM
Chemnitius
T
T
Manglitz
M
M
Kloeppel
T
T
Doenst
P
P
Schwartz
H
H
Kreuzer
R
R
Zech
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002950:Citrate (si)-Synthase / Q000378:metabolism; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure*; D009206:Myocardium / Q000648:ultrastructure*; D010101:Oxygen Consumption; D011817:Rabbits; D012508:Sarcolemma / Q000648:ultrastructure*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013552:Swine
D000244:Adenosine Diphosphate; D002950:Citrate (si)-Synthase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase