Effects of protein kinase C activation on norepinephrine-induced phosphatidylinositide hydrolysis in intact rat aorta.
The purpose of this study was to investigate the role of protein kinase C in the regulation of alpha 1-adrenoceptor-mediated phosphatidylinositide hydrolysis in intact vascular smooth muscle. Phorbol myristate acetate (0.1 and 1 microM) and staurosporine inhibited and potentiated, respectively, norepinephrine-induced inositol phosphate formation in intact rat aorta. In contrast, 30 microM prostaglandin F2 alpha, which activated protein kinase C to a similar magnitude as 1 microM phorbol myristate acetate, was without effect on norepinephrine-induced inositol phosphate formation. These results suggest that protein kinase C activated in response to physiologic agonists, but not in response to phorbol esters, may be compartmentalized within the smooth muscle cell.
Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, OH 45267-0575.
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Bazan
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Campbell
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Rapoport
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism; D015237:Dinoprost / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006868:Hydrolysis; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology*; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000470:Alkaloids; D010716:Phosphatidylinositols; D015237:Dinoprost; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate; D009638:Norepinephrine