Analysis of Epstein-Barr virus gene transcription in lymphoma induced by the virus in the cottontop tamarin by construction of a cDNA library with RNA extracted from a tumour biopsy.
Inoculation of the cottontop tamarin with Epstein-Barr virus (EBV) gives rise to the development of mono- and/or oligoclonal large cell malignant lymphoma. A cDNA library was generated with the RNA extracted from an EBV-induced tamarin lymphoma biopsy in order to study the transcripts expressed in the tumour tissue. Fifteen EBV-specific cDNA clones were localized in the corresponding viral genomic fragments. Among them, two correspond to the EBNA-2 gene, and two others to the latent membrane protein gene. The majority of the cDNA clones were localized in the BamHI A fragment which has not been associated with latent expression. Furthermore, cDNAs were also found from the BamHI D and I fragments. Sequence analysis of the cDNAs localized in BamHI A showed that they correspond to a rightward transcript in the BALF-3 region, with the one clone that was sequenced containing four exons and three introns. The above results were confirmed by testing three different biopsies with the rapid amplification of cDNA ends-PCR method.
Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, U.K.
C X
CX
Zhang
P
P
Lowrey
S
S
Finerty
A J
AJ
Morgan
D000818:Animals; D001483:Base Sequence; D001706:Biopsy; D004279:DNA, Viral / Q000235:genetics*; D015723:Gene Library; D005814:Genes, Viral / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000382:microbiology*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D012452:Saguinus; D014158:Transcription, Genetic / Q000235:genetics*; D014412:Tumor Virus Infections / Q000382:microbiology*
D004279:DNA, Viral; D012367:RNA, Viral