Glycoprotein III (clusterin, sulfated glycoprotein 2) in endocrine, nervous, and other tissues: immunochemical characterization, subcellular localization, and regulation of biosynthesis.
Specific antisera were raised against the A and B chains of glycoprotein III. Immunoblotting revealed that in adrenal medulla both chains migrate very closely together in two-dimensional electrophoresis. Both chains with slightly differing molecular sizes are found in several endocrine tissues and in brain, kidney, liver, and serum. The mRNA has an analogous widespread distribution. In primary cultures of chromaffin cells the level of message becomes significantly increased by treatment with histamine or 12-O-tetradecanoylphorbol 13-acetate/forskolin. However, the increase is small when compared with that of secretogranin II. The subcellular localization of glycoprotein III in endocrine organs and in the posterior pituitary was investigated by subcellular fractionation and immunoelectron microscopy. Glycoprotein III was found to be confined to the large dense-core vesicles of these organs. For a discussion of the function of glycoprotein III, its localization in these organelles has to be taken into account.
Department of Pharmacology, University of Innsbruck, Austria.
A
A
Laslop
H J
HJ
Steiner
C
C
Egger
M
M
Wolkersdorfer
S
S
Kapelari
R
R
Hogue-Angeletti
J D
JD
Erickson
R
R
Fischer-Colbrie
H
H
Winkler
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D051152:Clusterin; D004702:Endocrine Glands / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D007120:Immunochemistry; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D015345:Oligonucleotide Probes / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism*; D014018:Tissue Distribution
D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger