Energetics of nutrition and polyamine-related tumor growth alterations in experimental cancer.
The aim of this study was to evaluate whether food intake modulates experimental tumour growth by acute alterations in the energy state and blood flow of the tumour, and if so whether such changes are related to alterations in the enzyme ornithinedecarboxylase (ODC) and DNA synthesis. Inbred mice (C57BL/J) bearing a syngeneic undifferentiated and rapidly growing tumour were used. The tumour levels of high energy phosphates were measured in vivo by 31-P-NMR spectroscopy and biochemically following tissue extraction. DNA synthesis was estimated by measuring the incorporation of bromodeoxy-uridine into tumour DNA. Difluoro-methylornithine (DFMO) was used to inhibit ODC-activity. Tumour blood flow was estimated by a 132Xe local clearance technique. Tumour progression was associated with a significant decrease in tumour tissue high energy phosphates. Acute starvation decreased DNA-synthesis and tumour energy charge as well as its PCr/Pi which were rapidly normalised during subsequent refeeding. These changes were related to similar alterations in tumour blood flow. The inorganic phosphate (Pi) resonance and the resonances in the phosphomonoester (PME) region were considerably increased in tumour tissue. Inhibition of ODC-activity by DFMO decreased DNA-synthesis, which was associated with a secondary increase in tumour high energy phosphates probably due to a lowered energy demand for tumour cell division. The results demonstrate that host undernutrition was translated into retarded tumour growth associated with a decrease in the energy state and blood flow of the tumour. The results have bearing for the evaluation and planning of all treatment protocols with potential influence on food intake in experimental tumour-bearing animals.
Department of Otolaryngology, Sahlgrenska Hospital, University of Göteborg, Sweden.
T
T
Westin
B
B
Soussi
J P
JP
Idström
P
P
Lindnér
S
S
Edström
E
E
Lydén
B
B
Gustavsson
L
L
Hafström
K
K
Lundholm
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004435:Eating / Q000502:physiology*; D000518:Eflornithine / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D010759:Phosphorus Isotopes; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D013217:Starvation
D010759:Phosphorus Isotopes; D010725:Phosphocreatine; D010758:Phosphorus; D008748:Methylcholanthrene; D000255:Adenosine Triphosphate; D009955:Ornithine Decarboxylase; D000518:Eflornithine