Identification of Scyllo-inositol in proton NMR spectra of human brain in vivo.
Scyllo-inositol has been identified in proton NMR spectra of mammalian brain in vitro and in vivo. In contrast to myo-inositol this isomer comprises six equivalent CH protons that yield a singlet resonance at a chemical shift of 3.35 ppm. 1-D and 2-D J-resolved proton NMR studies (7.0 T) of perchloric acid extracts of brain tissues revealed different amounts of scyllo-inositol in man, sheep, cow and rat. Absolute quantification of localized short-echo time proton NMR spectra (2.0 T) of human brain in vivo resulted in scyllo-inositol concentrations of 0.35 +/- 0.06 mM for white matter (n = 25), 0.43 +/- 0.11 mM for grey matter (n = 23) and 0.57 +/- 0.14 mM for cerebellum (n = 10). Evidence for a tight metabolic link to myo-inositol was supported by a simultaneous variation of myo- and scyllo-inositol in patients with various brain diseases.
Michaelis
T
T
Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.
Helms
G
G
Merboldt
K D
KD
Hänicke
W
W
Bruhn
H
H
Frahm
J
J
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007294:Inositol / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011522:Protons; D051381:Rats; D012756:Sheep; D013057:Spectrum Analysis / Q000379:methods; D013237:Stereoisomerism
D011522:Protons; C009217:scyllitol; D007294:Inositol; D005947:Glucose