[Testing transgenic plants using the polymerase chain reaction].
A test system for selecting transgenic plants based on polymerase chain reaction (PCR) has been proposed. It is applicable to primary screening of transgenic plants obtained by cocultivation with Agrobacterium which contains any vector carrying neomycin phosphotransferase genes from transposon Tn5 and Streptococcus (for example pBIN19). These genes confer kanamycin resistance in plants and bacteria respectively. The absence of strong homology between these two genes allows one to perform two PCRs in the same reaction mixture. Thus simultaneous selection of transgenic plants and test for contamination with Agrobacterium are possible. We have also proposed a simple procedure for preparing small samples of plant DNA suitable for PCR detection.
Padegimas
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D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004251:DNA Transposable Elements; D005822:Genetic Vectors; D019868:Kanamycin Kinase; D007613:Kanamycin Resistance / Q000235:genetics; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics; D013291:Streptococcus / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*
D004251:DNA Transposable Elements; D004247:DNA; D010770:Phosphotransferases; D019868:Kanamycin Kinase