Defective T cell receptor-mediated signaling and differential induction of T cell functions by murine AIDS virus superantigen.
A B cell line, B6-1710, expressing murine AIDS virus superantigen stimulates T cell hybridomas derived from a Ld-specific CTL clone to produce IL-2 regardless of their CD4/CD8 phenotype. However, B6-1710 cell did not stimulate the original CTL clone, L3, in either proliferation or cytolytic assays. Both B6-1710 and Ld+ P815 cells stimulated a significant Ca2+ influx in the T cell hybridomas. In contrast, only P815 stimulated tyrosine phosphorylation of a 19-kDa protein in the T cell hybridoma. The addition of the nonspecific protein kinase C activator PMA restored the proliferative response of L3 cells to B6-1710. PMA did not induce the lysis of B6-1710 cells by L3. These results suggest that murine AIDS virus encoded superantigen elicits a TCR-mediated signal different from that caused by nominal Ag. This different signaling by viral superantigen may be important for the development of viral pathogenesis in vivo.
Department of Pathology and Medicine, Washington University School of Medicine, St. Louis, MO 63110.
O
O
Kanagawa
M E
ME
Wiebenga
B A
BA
Vaupel
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006825:Hybridomas / Q000276:immunology; D007519:Isoantigens / Q000276:immunology; D009052:Leukemia Virus, Murine / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016747:Minor Lymphocyte Stimulatory Antigens / Q000276:immunology; D016183:Murine Acquired Immunodeficiency Syndrome / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000956:Antigens, Viral; D007519:Isoantigens; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell; D013755:Tetradecanoylphorbol Acetate