Influence of aging on fluidity and coupling between beta-receptors and G-proteins in rat lung membranes.
We tested the hypothesis that in rat lung membranes, the age-related decline in the percentage of beta-receptors coupled with high affinity to G-proteins, is due to limitation of the diffusion caused by a decrease in membrane fluidity. We measured both parameters simultaneously in a crude membrane preparation from lungs of rat of different age. In contrast to what is found in crude membrane preparations from rat liver and brain, in rat lung fluidity was increased upon aging. We conclude that the age-related alteration in coupling between receptor and G-protein is difficult to explain by alterations of membrane fluidity.
Heymans Institute for Pharmacology, University of Gent, Belgium.
N
N
Fraeyman
P
P
Vanscheeuwijck
M
M
De Wolf
J
J
Quatacker
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D010743:Phospholipids; D011943:Receptors, Adrenergic, beta; D002784:Cholesterol; D019204:GTP-Binding Proteins