[Use of microwaves for enhancing or restoring antigens before immunohistochemical staining].
In the proposed protocol, the slides are deparaffinized, rehydrated, placed in 0.01 M, pH 6 citrate buffer and irradiated 3 x 5 minutes in a microwave oven (power output = 650 Watts) before immunostaining. This enables a positive staining for some antibodies and an enhancement of staining intensity for most antibodies.
Balaton
A J
AJ
Service d'Anatomie et de Cytologie Pathologiques, Hôpital Saint Joseph, Paris.
Ochando
F
F
Painchaud
M H
MH
D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000032:analysis*; D007158:Immunologic Techniques; D008872:Microwaves
D000906:Antibodies; D000941:Antigens