Use of Tn4560 to generate nikkomycin non-producing mutants of Streptomyces tendae.
Transposon Tn4560 was used to generate three nikkomycin non-producing mutants in Streptomyces tendae ATCC 31160. Southern hybridization confirmed that Tn4560 was present in 10-12-kb BamHI fragments of the chromosomes of the mutants. Biologically active nikkomycins were not detected in culture broths of the mutants as determined by bioassays and HPLC. Differences in the HPLC profiles of culture broths suggest that Tn4560 inserted into different genes in the mutants.
USDA, ARS, SRRC, New Orleans, Louisiana 70124.
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D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D004251:DNA Transposable Elements; D016254:Mutagenesis, Insertional; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; C011952:nikkomycin