Differential calcium mobilization by vasopressin, angiotensin II, gastrin-releasing peptide, and adenosine triphosphate in adult and fetal hepatocytes. Relevance for the activation of calcium-dependent enzymes.
Early signals elicited after membrane receptor binding of agonists, the transmembrane signaling pathway of which involves activation of phosphoinositide-specific phospholipase C, were compared in fetal (22 days gestation) and adult rat hepatocytes. Free cytosolic calcium changes varied depending on the agonist and type of stimulated cells. Angiotensin II and ATP elicited the maximal responses in both types of cells, whereas the maximal Ca2+ increase produced by vasopressin was twice as much in adult than in fetal hepatocytes. The opposite response was observed for bombesin- or gastrin-releasing peptide-stimulated cells. Triggering of fetal and adult hepatocytes with substances that maximally promote endoplasmic reticulum calcium release or phosphoinositide-specific phospholipase C activation revealed that at least for the actions mediated through the angiotensin II and P2 purinergic receptor, the agonist stimulation was near the maximal response capacity of the signaling pathway. Agreement was observed between the relative number of membrane receptors and the biological responses.
Junco
M
M
Instituto de Bioquímica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Díaz-Guerra
M J
MJ
Boscá
L
L
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D019886:Gastrin-Releasing Peptide; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism; D006005:Phosphorylases / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology; D010738:Type C Phospholipases / Q000378:metabolism; D014667:Vasopressins / Q000494:pharmacology*
D004075:Diglycerides; D010455:Peptides; D010716:Phosphatidylinositols; D012333:RNA, Messenger; D014667:Vasopressins; D000804:Angiotensin II; D019886:Gastrin-Releasing Peptide; D000255:Adenosine Triphosphate; D006005:Phosphorylases; D011493:Protein Kinase C; D010738:Type C Phospholipases; D002118:Calcium