Role of Porphyromonas gingivalis-derived fibroblast-activating factor in bone resorption.
A 24-kDa protein was isolated from the outer membrane vesicles of Porphyromaonas gingivalis W50. This protein, referred to as fibroblast-activating factor (FAF), was examined for its bone-resorptive ability by the rat long-bone assay and the mouse bone marrow cell culture system. FAF resulted in a significant release of 45Ca from cultured bones, as well as the formation of tartrate-resistant acid phosphatase-positive monocytes. These cultures were compared with control cells. FAF therefore might be considered a significant bacterially expressed protein which could affect and modulate the resorption or destruction of tissue and alveolar bone in the local periodontal environment.
Mihara
J
J
Dentistry for the Handicapped, Osaka University Dental Hospital, Japan.
Yoneda
T
T
Holt
S C
SC
D000818:Animals; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D001862:Bone Resorption / Q000139:chemically induced*; D010450:Endopeptidases; D005260:Female; D018093:Gelatinases; D006133:Growth Substances / Q000633:toxicity*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008813:Mice, Inbred ICR; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D011247:Pregnancy; D051381:Rats; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D010450:Endopeptidases; D018093:Gelatinases; D006133:Growth Substances; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha