Tri-primer PCR can distinguish free and cloned viral DNA in a single reaction.
A polymerase chain reaction based method is described which distinguishes free and cloned JC virus (JCV) DNA in a single reaction. The method utilizes three primers and amplifies DNA fragments of two different sizes, one specific for free and another specific for pBR322 cloned viral DNA. The procedure was used to simultaneously detect the presence of free and contaminating cloned JCV DNA in brain tissues.
Laboratory of Experimental Neuropathology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
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D001483:Base Sequence; D004274:DNA, Recombinant / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007577:JC Virus / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D004274:DNA, Recombinant; D004279:DNA, Viral