Use of cRNA digoxigenin-labelled probes for detection of enteroviruses in humans and in the environment.
A dot blot hybridization test for enteroviruses is described using non-radioactive digoxigenin-labelled probes and a chemiluminescent detection. The use of a 5' non-coding riboprobe which detects all enteroviruses and a VP1 probe that detects the three serotypes of polioviruses allows the rapid detection of polioviruses and non-polio enteroviruses in human specimens or environmental water samples. The assay is strictly enterovirus specific and sensitive (800 fg RNA) and offers several advantages over conventional diagnosis or radioactive probes.
Fuchs
F
F
Laboratoire de Virologie, Faculté de Médecine, Lyon, France.
Leparc
I
I
Kopecka
H
H
Garin
D
D
Aymard
M
M
D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004076:Digoxigenin; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics; D005602:France / Q000453:epidemiology; D006801:Humans; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization / Q000379:methods; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification; D015347:RNA Probes; D012367:RNA, Viral / Q000302:isolation & purification*; D014871:Water Microbiology
D036022:Capsid Proteins; D015347:RNA Probes; D012367:RNA, Viral; C442540:VP1 protein, Poliovirus; D004076:Digoxigenin