[Studies on the constituents of edible and medicinal plants. IV. Determination of 4-O-methylpyridoxine in serum of the patient with gin-nan food poisoning].
The serum 4-O-methylpyridoxine (MPN) levels in a 21 months-old patient with gin-nan food poisoning were determined by HPLC. The blood of the patient was taken at 8.5 and 15.5 h after taking about 50 ginkgo albumens. After deproteinization of the serum, the supernatant was pretreated with Sep-pak C18 cartridge and was applied to HPLC. HPLC was performed with a Hibar LiChrosorb RP-18 (4.0 mm i.d. x 250 mm, 7 microns) using a fluorescence detector (wave length of excitation and fluorescence; 290 and 400 nm, respectively). The serum MPN level was determined by the absolute calibration method. The determination limit of MPN in the serum was 0.05 micrograms/ml. The serum MPN concentration was 0.09 micrograms/ml at 8.5 h after taking ginkgo seeds, and was less than the determination limit of MPN (0.05 micrograms/ml) at 15.5 h. MPN, isolated from the seed of Ginkgo biloba L., is responsible for "gin-nan food poisoning," and its cardinal symptoms are mainly tonic and/or clonic convulsions and loss of consciousness. Infants are particularly vulnerable. This method may be available for proving the gin-nan food poisoning, chemically.
Yagi
M
M
Faculty of Pharmaceutical Sciences, Higashi Nippon Gakuen University, Hokkaido, Japan.
Wada
K
K
Sakata
M
M
Kokubo
M
M
Haga
M
M
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007223:Infant; D010939:Plant Poisoning / Q000097:blood*; D010945:Plants, Edible; D011736:Pyridoxine / Q000031:analogs & derivatives* / Q000097:blood; D012640:Seizures / Q000209:etiology*
C001737:4-methoxymethylpyridoxine; D011736:Pyridoxine