A three-dimensional plot for the display of transverse pore gradient gel electrophoresis data: application to a kinetoplast DNA fragment of planar circular conformation.
A 219 bp fragment of kinetoplast DNA from Crithidia fasciculata has previously been shown to exhibit a gel concentration dependent, anomalously low migration rate in 3-10% polyacrylamide transverse pore gradient gels relative to that of straight standards. The anomaly was interpreted in accordance with previous results of Marini et al. [1] as being due to the planar circular conformation of the fragment. The transverse pore gradient gel pattern is displayed here as a three-dimensional plot of migration distance vs. gel concentration and DNA length for the kinetoplast fragment and straight DNA standards. The plot yields a smooth surface for the standards clearly separated from the curve of the kinetoplast DNA fragment. Thus, transverse gradient gel electrophoresis in conjunction with the three-dimensional plot provides a unique method for the recognition of abnormal conformational features of DNA on the basis of electrophoretic mobility in a pore-size gradient.
Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-0001.
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Wheeler
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Chrambach
D000818:Animals; D016831:Crithidia fasciculata / Q000235:genetics*; D004270:DNA, Circular / Q000737:chemistry*; D016054:DNA, Protozoan / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D009690:Nucleic Acid Conformation
D004270:DNA, Circular; D016054:DNA, Protozoan