Genetic restriction of murine hepatitis virus type 3 expression in liver and brain: comparative study in BALB/c and C3H mice by immunochemistry and hybridization in situ.
To study the host-dependent genetic variations in murine hepatitis virus type 3 (MHV 3) induced diseases, we localized the sites of MHV 3 (Mill Hill strain) expression within liver and brain by immunohistochemistry or hybridization in situ. Two strains of mice were studied: BALB/c mice, which develop an acute and lethal hepatitis and C3H mice which develop a chronic brain infection. In BALB/c mice, viral RNA and antigens appeared during the first 24h post infection (p.i.) in liver, whereas viral RNA was barely detectable in brain, up until death at day 3 p.i. In C3H mice, viral RNA and antigens were detected simultaneously in liver and brain only at day 2 p.i. In brain, the virus was detected in meningeal and ependymal cells and in perivascular cortical areas (days 5 and 7 p.i.). After day 49, the virus was no longer detected in brain parenchyma, but persisted in meningeal cells. Two host-dependent genetic differences in viral processing were observed in the liver: (1) the virus was first detected in Kupffer cells in BALB/c mice and mostly in hepatocytes in C3H mice; (2) in BALB/c mice, the 180 kDa S viral glycoprotein appeared more frequently cleaved in 90 kDa form than in C3H mice.
Laboratoire de Neurovirologie et de Neuroimmunologie, Université Paris XI, France.
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Décimo
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Boespflug
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Meunier-Rotival
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Hadchouel
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Tardieu
D000818:Animals; D001921:Brain / Q000382:microbiology*; D003333:Coronaviridae Infections / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006524:Hepatitis, Viral, Animal / Q000235:genetics*; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008099:Liver / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D006517:Murine hepatitis virus / Q000302:isolation & purification*; D013045:Species Specificity