Evidence for a role for protein kinase C in the stimulation of protein synthesis by insulin in swiss 3T3 fibroblasts.
We have examined the role of protein kinase C (PKC) in the stimulation of protein synthesis by insulin using two complementary approaches. In the first, fibroblasts were pretreated with phorbol esters to down-regulate PKC. In these cells, the effects of insulin and of phorbol esters on protein synthesis were completely abolished, although serum still elicited an effect approaching that seen in control cells. Secondly, we used newly developed inhibitors of PKC which, again, blocked the effects of insulin and phorbol esters without greatly reducing the response to serum. Thus PKC apparently plays an important role in the stimulation of translation by insulin.
Welsh
G I
GI
Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, UK.
Proud
C G
CG
D016475:3T3 Cells; D000818:Animals; D020662:Guanine Nucleotide Exchange Factors; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D008301:Maleimides / Q000494:pharmacology; D051379:Mice; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism
D020662:Guanine Nucleotide Exchange Factors; D007211:Indoles; D007328:Insulin; D008301:Maleimides; D011506:Proteins; C071924:Ro 31-8425; D015240:Phorbol 12,13-Dibutyrate; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin; D011493:Protein Kinase C; C064758:Ro 31-8220; C064755:Ro 31-7549