Nucleotide sequence of gene 6 of avian-like group A rotavirus 993/83.
Two cDNA clones, which hybridized in Northern blots to RNA segment 6 of the homologous avian-like group A rotavirus (RV) 993/83 and pigeon RV PO-13, but not to mammalian group A rotaviruses, were sequenced. The gene 6 sequence contained a single long open reading frame encoding a protein of 397 amino acids (total MW 44,460). Comparison of the deduced amino acid sequence with VP6 protein from mammalian group A RV revealed 72 to 74.3% of amino acid identity. These values are surprisingly low in view of the high homology levels described up to now for group A RV. Avian-like RV 993/83 is however not a missing link between group A and C RV as it is as distantly related to group C RV as group A mammalian RV to group C RV. The evolutionary implications of these observations are briefly discussed.
Institute of Medical Microbiology and Virology, Ruhr-Universität Bochum, Germany.
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Rohwedder
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Irmak
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Werchau
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Brüssow
D000595:Amino Acid Sequence; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000145:classification / Q000235:genetics*; D036022:Capsid Proteins; D004279:DNA, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; C061332:VP6 protein, Rotavirus