N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) impairs neurotransmission in the vas deferens of the rat.
1. The in vitro effects of N-(2-chloroethyl)-N-ethyl-bromobenzylamine (DSP4) were studied in the rat vas deferens. 2. DSP4 inhibited the biphasic motor response induced by field stimulation in a concentration-dependent manner. The concentration of DSP4 that elicited 50% of the maximal inhibition of the twitch response induced by 3 Hz was 10 microM. 3. DSP4 10 microM abolished the motor response induced by exogenously applied noradrenaline and 0.1 mM ATP. Phentolamine (an alpha-adrenoceptor blocker) prevented DSP4 inhibitory effect. 4. DSP4 inhibitory effect was no due to the activation of alpha 2-presynaptic adrenoceptor mechanisms. 5. DSP4 impairs neurotransmission in the rat vas deferens by a postsynaptic alpha 1-adrenoceptor blockade and by an inhibition of the purinergic response.
Instituto de Investigaciones Farmacológicas, Consejo Nacional de Investigaciones Cientificas y Técnicas, Buenos Aires, Argentina.
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Landa
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Fiszman
D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D001596:Benzylamines / Q000494:pharmacology*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology*; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012110:Reserpine / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects*; D014649:Vas Deferens / Q000187:drug effects / Q000294:innervation*; D015016:Yohimbine / Q000494:pharmacology
D001596:Benzylamines; D014179:Neurotransmitter Uptake Inhibitors; D015016:Yohimbine; D012110:Reserpine; D000255:Adenosine Triphosphate; C012102:DSP 4; D009638:Norepinephrine; D010646:Phentolamine