Evidence that generation of inositol 1,4,5-trisphosphate and hydrolysis of phosphatidylinositol 4,5-bisphosphate are rapid responses following addition of fungal elicitor which induces phytoalexin synthesis in lucerne (Medicago sativa) suspension culture cells.
Treatment of lucerne suspension culture cells with glycoprotein elicitor from the phytopathogenic fungus Verticillium albo-atrum R & B triggers Ca(2+)-mediated induction of antimicrobial secondary metabolites termed phytoalexins. The present study investigated the possible role of polyphosphoinositide signal transduction in phytoalexin elicitation. Within 1 min of addition of elicitor to lucerne suspension culture cells we found a 100-160% (15-25 pmol/g fresh wt) increase in the level of compound with chromatographic and electrophoretic properties expected for an inositol trisphosphate (InsP3) and which was strongly bound by an inositol 1,4,5-trisphosphate (Ins(1,4,5)P3)-specific binding protein; after 3 min the level of this compound had fallen below that observed prior to elicitor challenge. In 32P-prelabelled cells, the relative proportion of radioactivity which cochromatographed with phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) was found to have decreased by 48% 1 min after elicitor addition and that rapid depletion of membrane lipid radioactivity was specific to this lipid fraction. The rapid, transient increase in level of Ins(1,4,5)P3 and concomitant fall in PtdIns(4,5)P2 suggests that Ins(1,4,5)P3 generated by hydrolysis of PtdIns(4,5)P2 may provide a Ca(2+)-mobilizing signal in phytoalexin elicitation in lucerne.
Walton
T J
TJ
Biochemistry Research Group, School of Biological Sciences, University College of Swansea, Wales, U.K.
Cooke
C J
CJ
Newton
R P
RP
Smith
C J
CJ
D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006868:Hydrolysis; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis*; D000455:Medicago sativa / Q000378:metabolism*; D008563:Membrane Lipids / Q000502:physiology; D003904:Mitosporic Fungi / Q000502:physiology*; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000502:physiology; D010935:Plant Diseases; D010936:Plant Extracts / Q000096:biosynthesis*; D012717:Sesquiterpenes; D015398:Signal Transduction / Q000187:drug effects*; D013729:Terpenes; D000094162:Phytoalexins
D008563:Membrane Lipids; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D010743:Phospholipids; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D015544:Inositol 1,4,5-Trisphosphate; D000094162:Phytoalexins