Localization of the genes encoding the catalytic subunits of protein phosphatase 2A to human chromosome bands 5q23-->q31 and 8p12-->p11.2, respectively.
The gene for the alpha isoform of the catalytic subunit of human protein phosphatase 2A (PPP2CA) was localized to chromosome 5 using somatic cell hybrids, and then more finely mapped to chromosome region 5q23-->q31 by in situ hybridization using a tritiated cDNA probe. The gene for the beta isoform of the catalytic subunit of this enzyme (PPP2CB) was mapped by the polymerase chain reaction to human chromosome 8 using somatic cell hybrids. Fluorescence in situ hybridization was then used to localize the PPP2CB gene to 8p12-->p11.2, using a mixture of three genomic probes that ranged from 3.5 to 8 kb in size. Finally, Southern blot analysis of somatic cell hybrid DNA suggested that a PPP2CB catalytic subunit pseudogene (PPP2CBP) is on chromosome 16.
Imperial Cancer Research Fund, Lincoln's Inn Fields, London, UK.
T A
TA
Jones
H M
HM
Barker
E F
EF
da Cruz e Silva
R E
RE
Mayer-Jaekel
B A
BA
Hemmings
N K
NK
Spurr
D
D
Sheer
P T
PT
Cohen
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D002895:Chromosomes, Human, Pair 5; D002898:Chromosomes, Human, Pair 8; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D054648:Protein Phosphatase 2; D051381:Rats
D004247:DNA; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2