Inhibition of in vitro aminoacylation and translation by RNA reactive antibodies.
Antibodies were raised against guanosine-BSA, GMP-BSA and tRNA-mBSA conjugates separately in rabbits. Binding characteristics of these antibodies to various RNAs were studied using a sensitive avidin-biotin micro ELISA. These antibodies inhibited in vitro aminoacylation of tRNA in a dose dependent manner. This inhibition was reversed by the addition of the respective homologous haptens thereby showing the specificity of these antibodies. In vitro translation of endogenous mRNAs in rabbit reticulocyte lysate was also inhibited by these antibodies in a dose dependent manner.
Kala
K C
KC
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Antony
A
A
D000215:Acylation / Q000187:drug effects; D000818:Animals; D000974:Antibodies, Antinuclear / Q000494:pharmacology*; D001666:Binding Sites, Antibody; D002474:Cell-Free System; D004305:Dose-Response Relationship, Drug; D006151:Guanosine / Q000276:immunology; D006157:Guanosine Monophosphate / Q000276:immunology; D066298:In Vitro Techniques; D011061:Poly A / Q000276:immunology; D014176:Protein Biosynthesis / Q000187:drug effects*; D012343:RNA, Transfer / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D051381:Rats
D000974:Antibodies, Antinuclear; D006151:Guanosine; D011061:Poly A; D006157:Guanosine Monophosphate; D012343:RNA, Transfer