[The inhibitory effects of Takusha on the formation, growth and aggregation of calcium oxalate crystals in vitro].
The inhibitory effects of Takusha on the formation, growth and aggregation of calcium oxalate crystals were estimated by the microplate method and the use of Coulter counter TAII in the diluted urine system and undiluted urine system. In the diluted urine system, the inhibitory effect on aggregation and growth was calculated from the changes in the number and volume of crystals by the seed crystal method. Takusha had a strong inhibitory effect on the aggregation and growth when the concentration was above 10 micrograms/ml. In measuring the metastable limit by the microplate method, Takusha had a mild inhibitory effect on the formation of crystals above the concentration of 1 mg/ml. In the undiluted urine system, after determining the metastable limit, the formation and growth of calcium oxalate crystals which precipitated in response to a load of sodium oxalate were measured. Takusha had a strong inhibitory effect on the formation and growth at the concentration of 0.1-1 mg/ml. Takusha had no glycosaminoglicans in the dimethylmethylene blue assay and had an inhibitory effect on aggregation and growth when its molecular weight was over 10,000. Therefore, Takusha might be useful for preventing stones in recurrent stone formers.
Department of Urology, Kanazawa Medical University.
K
K
Kawamura
M
M
Moriyama
C
C
Nakajima
K
K
Miyazawa
K
K
Suzuki
R
R
Tsugawa
D002129:Calcium Oxalate / Q000652:urine*; D003460:Crystallization; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D014545:Urinary Calculi / Q000517:prevention & control
D004365:Drugs, Chinese Herbal; D002129:Calcium Oxalate