Nuclear and kinetoplast DNA synthesis in Trypanosoma cruzi, autoradiographical study with DNA polymerase inhibitors.
DNA synthesis in epimastigote forms of Trypanosoma cruzi was studied autoradiographically by incorporation of [3H]thymidine in the nuclear and kinetoplast DNA. Both DNA were heavily labelled. Three eukaryotic DNA polymerase inhibitors (aphidicolin, aracytidine, and dideoxythimidine) were chosen to study the nuclear and kinetoplast DNA synthesis in vivo. Inhibition was mainly observed with the nuclear DNA.
Departmento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.
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Rojas
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Gutierrez
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Litvak
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Solari
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D001345:Autoradiography; D003561:Cytarabine / Q000494:pharmacology; D004270:DNA, Circular / Q000096:biosynthesis*; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan / Q000096:biosynthesis*; D015224:Dideoxynucleosides / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000235:genetics*
D004270:DNA, Circular; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D015224:Dideoxynucleosides; D019384:Nucleic Acid Synthesis Inhibitors; D003561:Cytarabine; C019336:2',3'-dideoxythymidine; D016590:Aphidicolin