[Relative hydrophobicity and other properties of lectins in aqueous binary phase polymeric systems].
The relative hydrophobicity of ten purified lectins preparations (SBA, LCA, PNA, PSA, STA, WGA, Con A, PHA-P, RCA-I and HPA) was determined using the aqueous two-phase polymeric system Ficoll 400-dextran 70 (13.53% - 11.59%, w/w in H2O, respectively). The linear correlation was found between the relative hydrophobicity of the lectin preparations and their hemagglutinating activity towards native rabbit erythrocytes (r2 = 0.925). Con A specific carbohydrate ligands added to the system decreased the relative hydrophobicity of lectin. Using cross partition, isoelectric points for the lectins were determined.
Guliaeva
N D
ND
Lakhtin
V M
VM
Zaslavskiĭ
B Iu
BIu
Iamskov
I A
IA
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects; D006386:Hemagglutination Tests; D037102:Lectins / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers; D011817:Rabbits; D012996:Solutions; D014867:Water
D037102:Lectins; D011108:Polymers; D012996:Solutions; D014867:Water