Purification of radiolabeled monoclonal antibodies to angiotensin-converting enzyme significantly improves specificity and efficacy of its targeting into the lung.
The aim of this study was to improve the labeling/purification procedures for monoclonal antibody (MoAb) to angiotensin-converting enzyme (ACE). MoAb 9B9 was very stable upon iodination at a wide range of iodogen concentrations and incubation times, and was also very stable upon storage, indicating the high technological potential of this MoAb. Radiolabeled MoAb 9B9 was purified by (i) adsorption chromatography on cellulose, (ii) HPLC (gel filtration) and (iii) affinity chromatography on ACE-Sepharose. The best result was obtained with cellulose: specificity of MoAb 9B9 accumulation in the lung increased 2-fold. We conclude that the phenomenon of specific lung accumulation of MoAb 9B9 may serve as an ideal (convenient, cheap and technological) assay system for evaluation of monoclonal antibody modification and labeling.
National Cardiology Research Center, Moscow, Russia.
H
H
Hiemisch
V
V
Gavrilyuk
E
E
Atochina
M
M
Slinkin
V
V
Torchilin
V
V
Muzykantov
S
S
Danilov
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification* / Q000378:metabolism; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004356:Drug Storage; D000922:Immunotoxins / Q000302:isolation & purification* / Q000378:metabolism*; D007205:Indium Radioisotopes; D008168:Lung / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D009928:Organ Specificity; D007703:Peptidyl-Dipeptidase A / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013667:Technetium; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D007205:Indium Radioisotopes; D013667:Technetium; D007703:Peptidyl-Dipeptidase A