Construction of a retrotransposition indicator sequence using a neomycin resistance-encoding gene containing a functional intron.
An intron from a Syrian hamster gene was inserted into a neo gene such that splicing of the neo gene mRNA results in the synthesis of active aminoglycoside phosphotransferase. The unspliced construct is inactive in Escherichia coli, but confers resistance to G418 when transfected into mouse and hamster cells. This selectable marker is designed to aid in the cloning and identification of genomic integration sites following retrotransposition.
Schwartz
D A
DA
Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston 29425.
Dahm
M W
MW
Bai
L
L
Carnie
S
S
Norris
J S
JS
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D006224:Cricetinae; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D005839:Gentamicins / Q000494:pharmacology; D007438:Introns; D019868:Kanamycin Kinase; D008647:Mesocricetus; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010770:Phosphotransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics*; D014169:Transformation, Bacterial
D004251:DNA Transposable Elements; D005839:Gentamicins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C010680:antibiotic G 418; D010770:Phosphotransferases; D019868:Kanamycin Kinase