Production of interleukin-5 by mouse astrocytes and microglia in culture.
Interleukin (IL)-5 was originally isolated as a B cell growth factor derived from T cells and is known as a multipotent cytokine. We found that IL-5 was detected in the cultures of microglia and astrocytes by both the enzyme-linked immunosolvent assay (ELISA) and bioassay with IL-5-dependent cell line, T88M. We also found that microglia and astrocytes expressed IL-5 mRNA. IL-5 receptor mRNA, however, was not detected in these cells. Stimulation with interferon-gamma increased expression of IL-5 in astrocytes and microglia. These results suggest that IL-5 produced by brain cells may be involved in the interaction between brain cells and immune cells in the brain.
Sawada
M
M
Division of Cell Biology, School of Medicine, Fujita Health University, Aichi, Japan.
Suzumura
A
A
Itoh
Y
Y
Marunouchi
T
T
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D015848:Interleukin-5 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015848:Interleukin-5; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate