Papillomas and carcinomas in transgenic rabbits carrying EJ-ras DNA and cottontail rabbit papillomavirus DNA.
Two transgenic rabbits (TRI and TRIII) that carried cottontail rabbit papillomavirus (CRPV) DNA alone were identified; another (TRII) carried both CRPV DNA and EJ-ras. TRI and TRIII developed extensive skin papillomas at about 1 month of age, and transcripts of CRPV DNA were detectable only in skin and/or papillomas. TRII developed extensive squamous carcinomas of the skin at a very early age. Transcription of both CRPV DNA and EJ-ras was found in the skin cancers. Thus, the tissue specificity of CRPV DNA expression in transgenic rabbits was the same as in virion-infected animals. The expression of EJ-ras could be dependent on the expression of certain CRPV genes and may be a critical cofactor of CRPV DNA in the progression of carcinomas.
Department of Comparative Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
X
X
Peng
R O
RO
Olson
C B
CB
Christian
C M
CM
Lang
J W
JW
Kreider
D000818:Animals; D030801:Animals, Genetically Modified / Q000033:anatomy & histology / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics; D012777:Cottontail rabbit papillomavirus / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D010212:Papilloma / Q000235:genetics; D011817:Rabbits / Q000033:anatomy & histology / Q000235:genetics*; D012867:Skin / Q000473:pathology*; D014018:Tissue Distribution; D014158:Transcription, Genetic
D004273:DNA, Neoplasm