Restriction enzyme analysis of bovine herpesvirus-1 DNA from an Indian isolate.
An Indian isolate of BHV-1 was analyzed using several restriction endonucleases. The restriction pattern of BHV-1 DNA with HindIII, BamHI and EcoRI was found to be similar to other reported restriction endonuclease patterns for respiratory isolates of BHV-1. Thus, this isolate could be grouped under BHV-1.1 (respiratory form of BHV-1). Few restriction endonucleases yielded distinct restriction profile of BHV-1 DNA. The total molecular size of BHV-1 DNA for the isolate studied was also estimated to be within 136 kb to 138 kb.
National Biotechnology Center, Indian Veterinary Research Institute, Izatnagar.
P K
PK
Gupta
A
A
Rai
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D007194:India; D008970:Molecular Weight
D004279:DNA, Viral; D004262:DNA Restriction Enzymes