The effects of endotoxin administration on blood amino acid concentrations: similarities with sepsis.
Following an acute endotoxin (LPS) administration (1 mg/kg body weight) to rats, there was a decrease in the blood concentration of most gluconeogenic amino acids, alanine, glycine, serine, threonine and proline. While the administration of the endotoxin induced no changes in the concentrations of aromatic amino acids, it decreased the concentration of both branched-chain amino acids (leucine, isoleucine and valine) and basic amino acids (lysine, arginine, histidine and ornithine). On a global basis, the endotoxin significantly decreased the total blood amino acid concentration 2 hrs. after the administration, the effect lasting as long as 8 hrs. after endotoxin treatment. This decrease was mainly associated with a lower blood concentration of essential amino acids. Rats induced septic, by cecal ligature and puncture, showed a blood amino acid pattern most similar to those acutely treated with LPS.
Departament de Bioquímica i Fisiologia, Facultat de Biologia, Universitat de Barcelona, Spain.
C
C
García-Martínez
M
M
Llovera
F J
FJ
López-Soriano
J M
JM
Argilés
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D002432:Cecum; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D007700:Kinetics; D008070:Lipopolysaccharides / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood*; D013997:Time Factors
D000596:Amino Acids; D004731:Endotoxins; D008070:Lipopolysaccharides