Purification and properties of calmodulin from Phymatotrichum omnivorum.
Mycelia of Phymatotrichum omnivorum obtained at 10 day intervals during 10 to 50 days of growth were used for isolating calmodulin, and studying its effect on glycogen synthase, phosphorylase, phosphorylase kinase, cyclic AMP phosphodiesterase and Ca++ATPase. Glycogen synthase was inhibited until the 30th day by calmodulin, whereas calmodulin obtained from the 40th day stimulated glycogen synthase activity and the 50th day sample had no effect. cAMP phosphodiesterase and Ca++ATPase of P. omnivorum were stimulated by the respective calmodulin. Molecular weight of the purified fungal calmodulin was approximately 18 kD as revealed by sodium dodecyl sulphate gel electrophoresis. Trifluoperazine, dibucaine and lidocaine inhibited calmodulin activity and calmodulin activation of PDE, respectively.
Department of Biology, Fisk University, Nashville, Tennessee 37208.
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Gunasekaran
D000252:Calcium-Transporting ATPases / Q000187:drug effects / Q000378:metabolism; D002147:Calmodulin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005658:Fungi / Q000737:chemistry* / Q000201:enzymology / Q000254:growth & development; D006006:Glycogen Synthase / Q000187:drug effects* / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000187:drug effects* / Q000378:metabolism; D010764:Phosphorylase Kinase / Q000187:drug effects / Q000378:metabolism; D006005:Phosphorylases / Q000187:drug effects* / Q000378:metabolism
D002147:Calmodulin; D006005:Phosphorylases; D006006:Glycogen Synthase; D010764:Phosphorylase Kinase; D010727:Phosphoric Diester Hydrolases; D000252:Calcium-Transporting ATPases