Calcium transport by basolateral membranes of diabetic rats.
The current study was designed to examine calcium transport across the basolateral membrane of the enterocyte of control and diabetic rats. The slope of initial rate of ATP-dependent calcium uptake was significantly greater in controls than in diabetic rats (P < 0.001). Kinetic analysis of ATP-dependent calcium uptake showed a maximum velocity (Vmax) of 0.19 +/- 0.02 and 0.37 +/- 0.01 mumol.g protein-1.-s-1 in diabetic and control rats, respectively (P < 0.01). Km values were similar. Insulin therapy and calmodulin added to freeze-thawed basolateral membranes vesicles stimulated Vmax of ATP-dependent calcium uptake in diabetic membranes to values close to those of controls without a change in Km values. In contrast, the C(++)-Na+ exchange process was similar in both control and diabetic rats. These results suggest that the ATP-dependent calcium uptake process is decreased in diabetic animals. This decrease is stimulated by calmodulin or insulin therapy, implying a causal relationship between insulin deficiency and calmodulin activity in diabetes.
Department of Pediatric Gastroenterology/Nutrition, Vanderbilt University Medical Center, Nashville, TN 37232-2576.
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D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000493:pharmacokinetics*; D002147:Calmodulin / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000201:enzymology / Q000378:metabolism*; D007583:Jejunum / Q000201:enzymology / Q000378:metabolism*; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008297:Male; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis
D002147:Calmodulin; D007328:Insulin; D012964:Sodium; D009251:NADPH-Ferrihemoprotein Reductase; D003576:Electron Transport Complex IV; D007931:Leucyl Aminopeptidase; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D002118:Calcium