New calibration method for gas chromatographic assay of carbon monoxide in blood.
The calibration method described is based on the in situ formation of carbon monoxide in a closed system by the reaction of hot concentrated sulfuric acid with formic acid. Carbon monoxide is released from hemoglobin by treatment with 85% phosphoric acid, diluted 1:2 (v/v). Carbon monoxide is analyzed by means of headspace gas chromatography on a Porapak Q 80-100 column, following catalytic reduction to methane, using a flame-ionization detector. The method was validated by comparing the results obtained for blood samples from patients suffering from carbon monoxide poisoning and previously analyzed by means of spectrophotometry.
Laboratoire de Chimie Analytique, Faculté des Sciences Pharmaceutiques, Université de Paris XI, Chatenay Malabry, France.
Z L
ZL
Cardeal
D
D
Pradeau
M
M
Hamon
I
I
Abdoulaye
F M
FM
Pailler
B
B
Lejeune
D002138:Calibration; D002248:Carbon Monoxide / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans
D002248:Carbon Monoxide