Inositol lipid-mediated intranuclear signalling: a comparative analysis of in vivo labelling in interferon alpha-sensitive and -resistant Daudi lymphoma cells.
Changes in inositol lipid and diacylglycerol metabolism have been analysed in Daudi lymphoma cells treated up to 24 h with human DNA recombinant interferon alpha. Results showing a different response of nuclear phosphoinositides and diacylglycerol, compared to whole cells, suggest that the intranuclear signalling system activated by interferon in Daudi cells involves nuclear inositol lipid metabolism. A well-characterized clone of Daudi cells selected for resistance to the antiproliferative action of interferon provided controls for the specificity of results.
Istituto di Morfologia Umana Normale, Università G. D'Annunzio, Chieti, Italy.
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D000293:Adolescent; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002051:Burkitt Lymphoma / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology*; D004075:Diglycerides / Q000378:metabolism; D015536:Down-Regulation; D004351:Drug Resistance; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000502:physiology*; D010766:Phosphorylation; D011994:Recombinant Proteins; D015290:Second Messenger Systems; D014407:Tumor Cells, Cultured
D004075:Diglycerides; D007370:Interferon Type I; D008563:Membrane Lipids; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D011994:Recombinant Proteins