Metabolic characteristics of pig colonocytes after adaptation to a high fiber diet.
The capacities of viable colonic epithelial cells to metabolize glucose, glutamine and n-butyrate were studied in 30-kg pigs adapted to a high fiber (12% sugar beet fiber) or a low fiber diet. Glucose and glutamine were extensively utilized but predominantly not oxidized, whereas n-butyrate oxidation accounted for 45% of n-butyrate metabolism and was not greatly affected by the presence of glucose or glutamine. With both diets, glycolysis was the major pathway accounting for glucose disappearance. There was a sparing effect of n-butyrate on both glycolysis and glucose oxidation. Moreover, the glycolytic capacity was 25% lower in pigs fed the high fiber diet. Data suggest that 6-phosphofructo-1-kinase could be the regulatory step in glycolysis. Nevertheless, its maximum activity was not affected by the diet or by the presence of n-butyrate. Glutamine metabolism was slightly affected by fiber in the diet and by the presence of n-butyrate. In addition to CO2, butyrate was converted into ketone bodies. Glucose and glutamine did not substantially alter n-butyrate metabolism. We conclude that some metabolic features of pig colonocytes, such as the capacity to oxidize n-butyrate, resemble those of rat and human colonocytes. Moreover, some characteristics, such as the glycolytic capacity, can be modulated by the level of fiber in the diet.
Darcy-Vrillon
B
B
Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy-en-Josas, France.
Morel
M T
MT
Cherbuy
C
C
Bernard
F
F
Posho
L
L
Blachier
F
F
Meslin
J C
JC
Duee
P H
PH
D020155:3-Hydroxybutyric Acid; D000818:Animals; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D003106:Colon / Q000378:metabolism*; D004043:Dietary Fiber / Q000008:administration & dosage*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D010084:Oxidation-Reduction; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013552:Swine
D002087:Butyrates; D004043:Dietary Fiber; D006885:Hydroxybutyrates; D007657:Ketone Bodies; D007773:Lactates; D005973:Glutamine; D020148:Butyric Acid; D019344:Lactic Acid; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D005947:Glucose; D020155:3-Hydroxybutyric Acid