Inhibition of the acute-phase response in a human hepatoma cell line.
The HuH-7 human hepatoma cell line was stimulated by IL-1 and IL-6 to increase the synthesis of acute-phase proteins, e.g. serum amyloid A (SAA), alpha 1 antichymotrypsin (ACT), alpha 1-protease inhibitor, alpha 1 acid-glycoprotein and haptoglobin, with the exception of the pentraxins (serum amyloid P and C-reactive protein). Haptoglobin and ACT were stimulated by IL-1 which has not been observed in some other hepatoma cell lines. The concentration of IL-1 required for stimulation of SAA was higher than that required for haptoglobin stimulation. IL-1 receptor antagonist was capable of inhibiting these responses and acted at a lower concentration to inhibit SAA than required to inhibit ACT or haptoglobin induction. Transforming growth factor beta (TGF beta) was also able to inhibit the response to IL-1 but had no effect on acute-phase protein responses to IL-6.
Molecular Immunology, London School of Hygiene and Tropical Medicine, UK.
J G
JG
Raynes
S
S
Bevan
D000210:Acute-Phase Reaction / Q000097:blood / Q000517:prevention & control*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D002460:Cell Line; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000494:pharmacology; D008113:Liver Neoplasms / Q000097:blood*; D000685:Serum Amyloid A Protein / Q000037:antagonists & inhibitors / Q000378:metabolism; D012795:Sialoglycoproteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014407:Tumor Cells, Cultured
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D000685:Serum Amyloid A Protein; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta