Whole mouse measurement of paramagnetism-loss of nitroxide free radical in lung with a L-band ESR spectrometer.
Paramagnetism-loss of nitroxide radical in lung of whole mouse was characterized using L-band ESR technique. The nitroxide, hydroxy-TEMPO, which had been injected trans-tracheally into mouse lung was confirmed to lose its paramagnetism in one electron reduction. The reduction activity remained even after lavage of lung. The reduction was inhibited by N-ethylmaleimide, a membrane-permeable sulfhydryl-blocker, though direct reduction of the radical by sulfhydryls was not observed in vitro. Sulfhydryl-blockers floating in membranes also inhibited the reduction, depending on their chain length. The results indicate that the reduction may be done within plasma membranes of alveolar epithelial cells.
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
K
K
Takeshita
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H
Utsumi
A
A
Hamada
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003497:Cyclic N-Oxides / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D005609:Free Radicals; D008168:Lung / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D013113:Spin Labels; D005033:Ethylmaleimide; C001803:tempol