Detection of free radicals in living mice after inhalation of DTBN by X-band ESR.
A new application of X-band electron spin resonance (ESR) is demonstrated for in vivo investigations on living mice. It is the first report on in vivo detection, identification, and localization of a free radical after uptake in the body of a living mammal by inhalation. The volatile di-tert-butyl-nitroxyl (DTBN) has been used as a suitable spin probe. After inhalation of DTBN, a superimposed ESR spectrum consisting of a polar (g = 2.0057; a = 1.65 mT) and a nonpolar paramagnetic species (g = 2.0061; a = 1.51 mT) was detected in mice tails. Collected blood exhibits only the polar spectrum. The decay of signals after removing of the DTBN-soaked swab follows first-order kinetics with k = 0.08 min-1.
Mäder
K
K
Department of Pharmacy, Humboldt University, Berlin, Germany.
Stösser
R
R
Borchert
H H
HH
D000280:Administration, Inhalation; D000818:Animals; D002073:Butanes; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals / Q000032:analysis* / Q000378:metabolism; D008297:Male; D051379:Mice; D009589:Nitrogen Oxides / Q000008:administration & dosage / Q000493:pharmacokinetics; D013113:Spin Labels; D013623:Tail
D002073:Butanes; D005609:Free Radicals; D009589:Nitrogen Oxides; D013113:Spin Labels; C007539:di-t-butyl nitroxide