The number of copies of an a-like region in the serotype-3 Marek's disease virus DNA genome is variable.
A 1.2-kilobase-pair DNA region (a-like) in the serotype-3 Marek's disease virus was directly repeated at the ends of the long (L) and short (S) components of the genome and repeated in the opposite orientation at the junction between the L and S components. Four variants from plaque-purified virus that varied in the number of copies of the a-like region were identified. The predominant variant contained one copy at both ends of the genome and at the L-S junction. Two other variants contained either one or two copies at the L end and one to three copies at the L-S junction. The fourth variant lacked the a-like region at the L end and the L-S junction. The fourth variant became the predominate species as the virus was serially passaged in cell culture.
USDA/Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
J D
JD
Reilly
R F
RF
Silva
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004279:DNA, Viral / Q000737:chemistry*; D005347:Fibroblasts; D017353:Gene Deletion; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004279:DNA, Viral