[Detection of hepatitis B virus DNA in hepatocellular carcinoma by in situ hybridization].
Surgical specimens from 27 patients with hepatocellular carcinoma (HCC) were studied by in situ hybridization with 3H-labelled HBV DNA probe for detecting HBV DNA and by immunohistochemical staining (PAP) for HBsAg and HBcAg on formalin-fixed, paraffin-embedded sections respectively. The positive rate of HBV DNA was 74.07% (20/27) in the peritumor liver tissues and 48.15% (13/27) in the tumorous tissues. HBV DNA was found mainly in the cytoplasm of the hepatocytes and cancer cells, and very few in the nucleus. HBV DNA granules were more in the cytoplasm than in the nucleus, and HBV DNA positive cells were much more in the peri-tumor tissues than in the tumor tissues. These results show that most of the HCC cases selected were closely associated with the infection of HBV. HBV DNA in the peri-tumor tissues might be of the replicative form, while in the cancerous tissues, it might be of the integrated form. The relationship between HBV DNA and HBsAg, HBcAg in the liver tissues is discussed.
Department of Pathology, Yangzhou Medical College.
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D000293:Adolescent; D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000276:immunology / Q000382:microbiology; D008113:Liver Neoplasms / Q000382:microbiology*; D008297:Male; D008875:Middle Aged
D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens