HPV DNA presence and HPV genotypes as prognostic factors in low-stage squamous cell cervical cancer.
Sixty-four patients with FIGO stages IB and IIA squamous cell carcinoma of the uterine cervix were studied to assess the prognostic potential of the presence of HPV DNA and HPV genotypes in the tumor, as detected by a general primer-mediated polymerase chain reaction followed by a type-specific PCR on paraffin-embedded tissue sections of the primary tumor. The prevalence of various HPV types in 32 patients with positive lymph nodes was compared to the prevalence found in 32 age-matched controls with negative lymph nodes. In both patient groups HPV DNA was found in all cases. The prevalence rates of HPV types 16, 18, and X in the positive and negative lymph node group were 78% versus 72%, 13% versus 9%, and 3% versus 16%, respectively. These differences were not statistically significant. HPV genotypes 6, 11, and 31 were not found. Differences in recurrence rate and 5-year survival rate between the patient groups with different tumor-containing HPV genotypes were not statistically significant. Nor was there any statistically significant relationship between HPV type and other well-known prognosticators. In conclusion, in our study a 100% HPV DNA prevalence was found in squamous cell cervical carcinomas. Neither the presence of HPV DNA nor the HPV genotype appeared to be related to either the presence of lymph node metastases at the time of initial treatment or to 5-year survival.
Department of Obstetrics and Gynecology, Free University Hospital, Amsterdam, The Netherlands.
P F
PF
van Bommel
A J
AJ
van den Brule
T J
TJ
Helmerhorst
M P
MP
Gallee
K N
KN
Gaarenstroom
J M
JM
Walboomers
C J
CJ
Meijer
P
P
Kenemans
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000382:microbiology* / Q000401:mortality; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011379:Prognosis; D016019:Survival Analysis; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology* / Q000401:mortality
D014408:Biomarkers, Tumor; D004279:DNA, Viral