Separable regulatory elements governing myogenin transcription in mouse embryogenesis.
Expression of the myogenic helix-loop-helix (HLH) protein myogenin in muscle cell precursors within somites and limb buds is among the earliest events associated with myogenic lineage determination in vertebrates. Mutations in the myogenin promoter that abolish binding sites for myogenic HLH proteins or myocyte enhancer factor-2 (MEF-2) suppressed transcription of a linked lacZ transgene in subsets of myogenic precursors in mouse embryos. These results suggest that myogenic HLH proteins and MEF-2 participate in separable regulatory circuits leading to myogenin transcription and provide evidence for positional regulation of myogenic regulators in the embryo.
Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
T C
TC
Cheng
M C
MC
Wallace
J P
JP
Merlie
E N
EN
Olson
D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism*; D005121:Extremities / Q000196:embryology; D005260:Female; D064326:MEF2 Transcription Factors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D009124:Muscle Proteins / Q000235:genetics*; D009132:Muscles / Q000196:embryology* / Q000378:metabolism; D009154:Mutation; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D064326:MEF2 Transcription Factors; D009124:Muscle Proteins; C503801:Myog protein, mouse; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D015534:Trans-Activators; D014157:Transcription Factors