Identification of a linear neutralization site within the third variable region of the feline immunodeficiency virus envelope.
Synthetic peptides have been used to map linear B-cell epitopes of the third variable (V3) region of the feline immunodeficiency virus (FIV) external membrane glycoprotein gp120. The analysis of sera from naturally and experimentally FIV-infected cats by Pepscan and enzyme immunoassay with four partially overlapping peptides evidenced three antibody-binding domains, two of which mapped in the carboxyl-terminal half of V3. In particular, the V3.3 sequence (Gly-392-Phe-413) turned out to be important for in vitro neutralization of the virus in that the peptide inhibited the FIV-neutralizing activity of pooled immune cat sera, and on the other hand, cat sera raised against this peptide effectively neutralized FIV infectivity for Crandell feline kidney cells.
Lombardi
S
S
Department of Biomedicine, University of Pisa, Italy.
Garzelli
C
C
La Rosa
C
C
Zaccaro
L
L
Specter
S
S
Malvaldi
G
G
Tozzini
F
F
Esposito
F
F
Bendinelli
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D000917:Antibody Formation; D002415:Cats / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015686:Gene Products, env / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D017433:Protein Structure, Secondary; D013997:Time Factors
D000914:Antibodies, Viral; D015686:Gene Products, env; D007106:Immune Sera; D010455:Peptides