[The morphofunctional instability of the lymphocyte membranes in patients with chronic kidney failure].
A rosette test, fluorescent probing (1-aniline-naphthaline-8-sulfonate and pyrene) and 14C-uridine inclusion into immuno-competent cells were used to investigate T-lymphocyte membrane E-receptor activity, mobility of the surface and deep parts of lymphocytic membrane lipid bilayer, and membrane transport, respectively. A total of 16 patients with conservatively curable and 31 patients with a terminal stage of chronic renal failure (CRF) were examined. The lymphocytic membranes were found unstable: E-receptor activity of RFC changed, microviscosity of the surface and deep lipid bilayer diminished, the transport function got disturbed. These phenomena significantly progress with deterioration of renal function. It is evident that morphofunctional damage to lymphocytic membranes may be a reason of lymphocytopenia and secondary immunodeficiency in CRF patients.
Greinshteĭn
Iu I
IuI
Osetrov
I V
IV
Tereshchenko
V P
VP
D002462:Cell Membrane / Q000502:physiology; D004912:Erythrocytes / Q000502:physiology; D005456:Fluorescent Dyes; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000209:etiology; D008051:Lipid Bilayers / Q000097:blood; D008560:Membrane Fluidity; D011956:Receptors, Cell Surface / Q000502:physiology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000502:physiology*; D014783:Viscosity
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D011956:Receptors, Cell Surface