In vitro selection of Junin virus antigenic variants.
The establishment of an experimental persistent infection with Junin virus, the aetiological agent of argentine hemorrhagic fever, involves the emergence of antigenic variants in brain and blood of the cricetid Calomys musculinus. We demonstrate that antigenic variants can also be isolated in vitro under the selective pressure of polyclonal antibodies and from a long-term infected C. musculinus primary embryo fibroblast culture. The participation of neutralizing antibodies and host cells in the appearance of viral variants in vivo is discussed.
Alché
L E
LE
Department of Biochemistry, School of Science, University of Buenos Aires, Argentine.
Coto
C E
CE
D000818:Animals; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D006477:Arenaviruses, New World / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D003411:Arvicolinae; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D006478:Hemorrhagic Fever, American / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D014709:Vero Cells
D000956:Antigens, Viral; D007106:Immune Sera