HLA-unrestricted killing of HSV-1-infected mononuclear cells. Involvement of either gamma/delta+ or alpha/beta+ human cytotoxic T lymphocytes.
The characterization of HSV-specific human CTL, obtained in short term cultures by stimulating PBMC of healthy HSV-immune donors with autologous, PHA-activated, HSV-1-infected mononuclear cells, is described. CTL induced by using this technique are able to mediate a strong lytic activity against both HSV-1- and HSV-2-infected targets, whereas they do not kill autologous EBV-lymphoblastoid cell lines unless they are superinfected with HSV-1. TCR-gamma/delta+ cells are mainly responsible for HSV-specific cytotoxic activity in some donors, whereas TCR-alpha/beta+ CTL are primarily involved in other subjects. The large majority of HSV-specific CTL bearing either TCR-gamma/delta or TCR-alpha/beta also express CD8 and/or CD56 molecules. Virus-specific CTL, here described, require the expression of HLA class I Ag on the surface of target cells to mediate lytic activity. Nevertheless, the response is apparently HLA-unrestricted in that HSV-1-induced CTL are also able to lyse target cells mismatched for A, B, C, DR, and DQ loci. Our data suggest that both TCR-gamma/delta+ and TCR-alpha/beta+ CTL may play a role in the immune response to HSV in humans.
Department of Pediatrics, University of Pavia, IRCCS Policlinico San Matteo, Italy.
R
R
Maccario
M G
MG
Revello
P
P
Comoli
D
D
Montagna
F
F
Locatelli
G
G
Gerna
D015703:Antigens, CD / Q000032:analysis; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000276:immunology*; D006561:Herpes Simplex / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000382:microbiology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D018139:Simplexvirus / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015703:Antigens, CD; D006680:HLA Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta