Endothelial damage of the venous graft in CABG. Influence of solutions used for storage and rinsing on endothelial function.
A significant proportion of early graft occlusions after aortocoronary revascularization using autologous saphenous vein grafts (SVG) are due to mechanical and/or metabolic or biochemical endothelial lesions. The morphological examination of the endothelium, usually carried out using light microscopy or by various types of scanning electron microscopy (SEM), does not give any indication of the functioning of the endothelium (E). Functionally intact E is capable of producing endothelium-derived relaxing factor (EDRF); a practicable in vitro test is the relaxation of pre-contracted vein segments (VS) in response to acetylcholine (ACh) application. To study the effect of the solution used to rinse and store the SVG between removal and implantation on the functional characteristics of the E, we performed in vitro tests on macroscopically intact VS removed from the saphenous vein of 30 male patients who underwent elective CABG surgery. Isolated VS rings were incubated for 60 min in heparinized whole blood (HWB), Bretschneider's cardioplegic solution (HTK), human albumin solution (HAS), or Ringer's solution (RS) and compared with the results obtained immediately after the removal of untreated control samples (C) taken from the same patients. After equilibration in carbogen aerated Krebs-Henseleit solution and precontraction by 3 x 10(-7) M noradrenaline (NE), relaxation induced by 10(-6) M ACh was measured. Only the samples stored in HWB (13.4 +/- 0.4 mN) showed similar maximal contractions with NE to those in the control group (14.4 +/- 0.5 mN), i.e. all those segments which showed both contractions with NE and relaxation with ACh.(ABSTRACT TRUNCATED AT 250 WORDS)
Universitätsklinikum Essen, Germany.
H R
HR
Zerkowski
M
M
Knocks
M A
MA
Konerding
N
N
Doetsch
G
G
Roth
K
K
Hakim
J C
JC
Reidemeister
D000109:Acetylcholine; D000328:Adult; D000368:Aged; D000418:Albumins; D002314:Cardioplegic Solutions; D001026:Coronary Artery Bypass; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D005947:Glucose; D006493:Heparin; D006801:Humans; D007552:Isotonic Solutions; D008297:Male; D008353:Mannitol; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009569:Nitric Oxide / Q000096:biosynthesis; D009638:Norepinephrine; D009926:Organ Preservation / Q000379:methods; D011189:Potassium Chloride; D011343:Procaine; D000077331:Ringer's Solution; D012501:Saphenous Vein / Q000473:pathology* / Q000637:transplantation*; D014325:Tromethamine
D000418:Albumins; C039984:Bretschneider cardioplegic solution; D002314:Cardioplegic Solutions; D007552:Isotonic Solutions; C074097:Krebs-Henseleit solution; D014325:Tromethamine; D009569:Nitric Oxide; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D000077331:Ringer's Solution; D006493:Heparin; D005947:Glucose; D000109:Acetylcholine; D009638:Norepinephrine