NADP-linked isozymes are the major forms of isocitrate dehydrogenase in mouse type-1-like astrocytes.
The activities of cytosolic NADP-linked and mitochondrial NADP- and NAD-linked isocitrate dehydrogenases were examined in three-week-old mouse astroglial cultures. The respective activities were found to be 57 +/- 7, 101 +/- 3 and 8 +/- 1 nmoles NAD(P)+ reduced per mg protein per min. Both dibutyryl cyclic AMP and cortisol caused a significant increase in the activity of the mitochondrial as well as cytosolic NADP-linked isozymes.
Department of Anatomy, University of Saskatchewan, Saskatoon, Canada.
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Juurlink
D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D003994:Bucladesine / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D007521:Isocitrate Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism*
D007527:Isoenzymes; D009243:NAD; D009249:NADP; D003994:Bucladesine; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D006854:Hydrocortisone