Interferon-alpha and dexamethasone effect on AF10 myeloma cell line sialytransferase activity.
Interferon-alpha and the synthetic glucocorticoid dexamethasone are used in the treatment of multiple myeloma. The effect of 48 h exposure to recombinant interferon-alpha 2b (100 to 10,000 U/ml) and dexamethasone (1 microM to 1 mM) was studied in AF10, and IgE-secreting myeloma cell line. Interferon-alpha and dexamethasone at 10,000 U/ml and 1 mM respectively had a synergistic inhibitory effect on IgE concentration. Interferon-alpha and dexamethasone had an additive inhibitory effect on myeloma cell concentration. Interferon-alpha at 10,000 U/ml caused a twofold increase in sialyltransferase activity. Dexamethasone decreased sialytransferase activity and attenuated the stimulatory effect of interferon-alpha. These data suggest that combined interferon-alpha and dexamethasone therapy might have a synergistic effect on monoclonal protein production and a diverse effect on sialytransferase activity.
Cohen
A M
AM
Hematology Clinic, Golda Medical Center, Petah-Tikya, Israel.
Wacks
T
T
Lurie
B B
BB
Levinsky
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins; D012799:Sialyltransferases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D003907:Dexamethasone; D012799:Sialyltransferases