Inositol lipid-mediated intranuclear signalling: a comparative analysis of in vivo labelling in interferon alpha-sensitive and -resistant Daudi lymphoma cells.
Changes in inositol lipid and diacylglycerol metabolism have been analysed in Daudi lymphoma cells treated up to 24 h with human DNA recombinant interferon alpha. Results showing a different response of nuclear phosphoinositides and diacylglycerol, compared to whole cells, suggest that the intranuclear signalling system activated by interferon in Daudi cells involves nuclear inositol lipid metabolism. A well-characterized clone of Daudi cells selected for resistance to the antiproliferative action of interferon provided controls for the specificity of results.
Cataldi
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A
Istituto di Morfologia Umana Normale, Università G. D'Annunzio, Chieti, Italy.
Di Primio
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Lisio
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Rana
R A
RA
Robuffo
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I
Bosco
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D
Miscia
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S
D000293:Adolescent; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002051:Burkitt Lymphoma / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology*; D004075:Diglycerides / Q000378:metabolism; D015536:Down-Regulation; D004351:Drug Resistance; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000502:physiology*; D010766:Phosphorylation; D011994:Recombinant Proteins; D015290:Second Messenger Systems; D014407:Tumor Cells, Cultured
D004075:Diglycerides; D007370:Interferon Type I; D008563:Membrane Lipids; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D011994:Recombinant Proteins