Establishment and characterization of a human mixed-lineage, T-lymphoid/myeloid cell line (USP-91).
We report the establishment of a novel cell line from a pediatric patient with recurrent non-Hodgkin's lymphoma. This cell line, termed USP-91, showed both T-lymphoid cell as well as myeloid (ie, nonlymphoid) cell characteristics using a comprehensive multiparameter approach. The initial growth of this cell line was dependent on the presence of the murine stromal cell line, 14F1.1. Subsequently, a phenotypically stable, stroma-independent cell line was established. Although the recurrent biopsy material and the derivative cell line, USP-91, were clonally-derived from T-lineage lymphoid cells, as evidenced by the same rearrangement of the T-cell receptor-beta locus, USP-91 coexpressed both the T-cell antigens CD7, CD3, and CD4, and the myeloid antigens CD13, CD33, CD11b, and CD34. The myeloid features of USP-91 were most consistent with monocytic differentiation as these cells expressed alpha-napthol acetate esterase, lysozyme, alpha-1-antitrypsin, alpha-1-antichymotrypsin, as well as the cell surface receptor for macrophage colony-stimulating factor. In addition, incubation in the presence of phorbol esters induced USP-91 to exhibit morphologic and functional properties of mature mononuclear phagocytes. The expression of this bilineage phenotype suggests that USP-91 represents the malignant transformation of a progenitor cell capable of either myelomonocytic or T-lymphoid differentiation.
Department of Pathology, Childrens Hospital, Los Angeles, CA 90027.
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T
Umiel
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Rettenmier
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Siegel
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Ota
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Shimada
T W
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Tran
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Pattengale
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001706:Biopsy; D002460:Cell Line; D002675:Child, Preschool; D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods; D015321:Gene Rearrangement; D015329:Gene Rearrangement, T-Lymphocyte; D005803:Genes, Immunoglobulin; D006801:Humans; D007621:Karyotyping; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D012008:Recurrence; D012878:Skin Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure
D015703:Antigens, CD