Cyclic AMP regulation of the human choline acetyltransferase gene.
Regulation of the human choline acetyltransferase gene by 8-bromo-cAMP was investigated by transfecting into NS-20Y cells choline acetyltransferase promoter sequences fused to the reporter gene firefly luciferase. Promoter activity was localized to nucleotides -163 to +73. This region of the gene responded to 8-bromo-cAMP in a similar fashion as the endogenous enzyme. However, nearly the same induction was observed for constructs containing unrelated promoters. Furthermore, fusion of the choline acetyltransferase gene to the thymidine kinase promoter yielded no increased induction by 8-bromo-cAMP. These results suggest that cAMP regulates choline acetyltransferase gene transcription through an indirect mechanism.
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 79235.
Y P
YP
Li
E E
EE
Baetge
L B
LB
Hersh
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000235:genetics*; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006678:HIV / Q000235:genetics; D008156:Luciferases / Q000235:genetics; D051379:Mice; D009447:Neuroblastoma; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP; D008156:Luciferases; D002795:Choline O-Acetyltransferase; D013937:Thymidine Kinase