Glucocorticoid requirement for growth of human papillomavirus 16-transformed primary rat kidney epithelial cells: correlation of development of hormone resistance with viral RNA expression and processing.
The establishment of transformation of primary baby rat kidney epithelial cells by human papillomavirus type 16 DNA requires glucocorticoid hormones (Pater et al., Nature (Lond.), 335: 832-835, 1988). In this report we provide evidence that growth of transformed baby rat kidney cells in culture also requires glucocorticoids. However, transformed cells for which growth does not require hormone readily arise after a brief period of crisis, if cultured without added hormone. No reduction of glucocorticoid receptor was evident in non-hormone-requiring cells. The expression of human papillomavirus 16 RNA in these cells was analyzed by Northern blot, primer extension, and RNase protection analysis. Cells that do not require hormone had greatly reduced levels of transcripts initiated from the viral P97 promoter. However, there is evidence for compensating alterations to allow more efficient expression of E7 mRNA, since the growth of these cells is correlated with altered patterns of viral RNA expression and processing.
Pater
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Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Belaguli
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Pater
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D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D004848:Epithelium; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007668:Kidney / Q000473:pathology; D027383:Papillomaviridae / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis; D051381:Rats
D004279:DNA, Viral; D005938:Glucocorticoids; D012367:RNA, Viral