Chemical structure of the 2-keto-3-deoxyoctonate region of lipopolysaccharide isolated from Porphyromonas (Bacteroides) gingivalis.
Structural analysis of the 2-keto-3-deoxyoctonate region of lipopolysaccharide (LPS) isolated from Porphyromonas (Bacteroides) gingivalis was carried out. The substitution of the polysaccharide portion on the KDO was determined by gas chromatography/mass spectrometry of the product obtained by sequential derivatization of the LPS, including dephosphorylation, permethylation, carboxyl reduction, partial hydrolysis, carbonyl reduction, complete hydrolysis and O-acetylation. It was revealed that the KDO carries the polysaccharide on its position C5 and is phosphorylated on either position C7 or C8, although its exact position is not determined. The structure of the KDO region of P. gingivalis LPS in Gram-negative bacterial LPS had not hitherto been elucidated.
Department of Oral Microbiology, Kanagawa Dental College, Japan.
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H
Kumada
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S
Kondo
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T
Umemoto
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Hisatsune
D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D016966:Porphyromonas gingivalis / Q000737:chemistry*; D013400:Sugar Acids / Q000737:chemistry*
D008070:Lipopolysaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate