A long-term suppression by alpha-interferon of Philadelphia chromosome in children with chronic myelogenous leukemia.
An 8 year old girl with adult type Philadelphia (Ph1)-positive chronic myelogenous leukemia received natural alpha-interferon therapy in the chronic phase. Complete suppression of the Ph1 clone of bone marrow cells was achieved after 1 month of therapy, which was determined by disappearance of rearranged breakpoint cluster region (BCR) gene in Southern blot analysis. Complete hematological remission was also attained following 2 months of therapy. Both the suppression and the hematological remission have been sustained for 24 months with alpha-interferon, in spite of the detection of the chimeric BCR/ABL mRNA in her bone marrow by polymerase chain reaction assay 12 months after therapy.
Department of Pediatrics, Fukui Medical School, Japan.
Y
Y
Kikawa
Y
Y
Fuji
T
T
Takano
Y
Y
Shigematsu
M
M
Sudo
M
M
Okamoto
S
S
Mizutani
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D001856:Bone Marrow Examination; D002648:Child; D004247:DNA; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000187:drug effects* / Q000235:genetics; D015321:Gene Rearrangement / Q000187:drug effects* / Q000235:genetics; D016313:Genes, abl / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology / Q000627:therapeutic use*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000628:therapy*; D015466:Leukemia, Myeloid, Chronic-Phase / Q000235:genetics / Q000628:therapy*; D008969:Molecular Sequence Data; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D012074:Remission Induction
D016898:Interferon-alpha; D012333:RNA, Messenger; D004247:DNA; D016044:Fusion Proteins, bcr-abl