Versatile adeno-associated virus 2-based vectors for constructing recombinant virions.
We have constructed several plasmid vectors with which a more efficient molecular cloning, followed by rescue, replication, and packaging of DNA fragments, can be achieved. The availability of these vectors should facilitate construction of a variety of recombinant adeno-associated virus 2 (AAV)-based virions containing therapeutic genes for potential use in human gene therapy.
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202-5120.
P
P
Nahreini
M J
MJ
Woody
S Z
SZ
Zhou
A
A
Srivastava
D015139:Blotting, Southern; D003001:Cloning, Molecular; D000229:Dependovirus / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005822:Genetic Vectors; D009355:Neomycin / Q000494:pharmacology; D010957:Plasmids; D014771:Virion / Q000235:genetics*
D009355:Neomycin