Oligonucleotide DNA fingerprinting: results of a multi-center study on reliability and validity.
We report the results of an empirical study of 256 paternity cases referred to 7 different German laboratories for DNA fingerprinting with oligonucleotide probe (CAC)5/(GTG)5. All parameters characteristic of such multilocus DNA fingerprints were found to differ significantly between the contributing centres. Despite these differences, clear-cut decisions between paternity and non-paternity could be made in all but one case. Furthermore, we found no systematic deviation of the gel-phenotype distribution among trios from random expectation as derived from commonly adopted analytical models. Thus, we conclude that oligonucleotide DNA fingerprinting is a robust and reliable means for the resolution of paternity cases.
Labor für Genetische Diagnostik, München, Germany.
I
I
Böhm
M
M
Krawczak
P
P
Nürnberg
J
J
Hampe
J
J
Hundrieser
H
H
Pöche
C
C
Peters
R
R
Slomski
J
J
Kwiatkowska
M
M
Nagy
D001483:Base Sequence; D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D010334:Paternity; D015203:Reproducibility of Results
D015345:Oligonucleotide Probes; D004247:DNA