[Expression of major histocompatibility complex antigens, and kinetics of infiltrated lymphocytes in murine herpetic keratitis].
Immunohistochemical techniques were used to study expression of HSV-1 antigen, induction of class I and II major histocompatibility complex (MHC) antigens, and infiltration of L3T4+ and Lyt2+ cells were analyzed during the acute phase of herpetic keratitis. The results were as follows: 1) HSV-1 antigens were detectable in the corneal epithelium at 2 days post infection (p.i.), then localized in the stroma after 5 days p.i.. 2) Class I MHC antigens (H-2d) were evenly expressed in all layers of infected as well as uninfected corneas. On the other hand, class II MHC antigens (Iad) were induced in the superficial corneal stroma 2 days p.i., then gradually spread in the entire corneal stroma with increased intensity. 3) L3T4+ (helper/inducer) cells penetrated the stroma at 2 days p.i.. These cells infiltrated the central stroma at 5 days p.i.. Lyt2+ (suppressor/killer) cells were first seen in the corneal stroma as late as at 5 days p.i.. These results imply that L3T4+ cells play a role in the early immunological events concerning class II MHC antigens and viral antigens, both concomitantly expressed in the corneal stroma.
Department of Ophthalmology, Ehime University School of Medicine, Japan.
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Uno
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Hayashi
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Ohashi
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007111:Immunity, Cellular; D007150:Immunohistochemistry; D016849:Keratitis, Herpetic / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II