Splenic macrophage activation and functions in amyloid enhancing factor-induced secondary amyloidosis. Study of phagocytosis, killing, respiratory burst, and MHC class II surface expression.
Secondary amyloidosis is a systemic disease characterized by the extracellular tissue deposition of insoluble fibrillar amyloid A protein. Aberrant metabolism of serum amyloid A protein by reticuloendothelial cells is thought to result in the accumulation of fibrils within the tissue. Treatment of mice with amyloid-enhancing factor (AEF) in conjunction with an inflammatory stimulus (i.e., AgNO3) induced amyloid deposition within 48-72 h. The activation state of a macrophage largely defines its enzymatic capabilities. In the studies reported here, we examined the effect of AEF on spleen macrophage activation using both functional and phenotypic assays. We found that while AEF in the presence or absence of AgNO3 has no apparent effect on the ability of spleen and liver macrophages to phagocytose or kill Listeria monocytogenes, it appears to block enhanced respiratory burst function (as measured by O2- production) observed with AgNO3 alone. AEF therefore seems capable of inhibiting certain macrophage activation-associated functions while not affecting others. Our activation phenotype studies, using surface Ia expression, reveal that AEF blocks the increase in number of splenic macrophages expressing Ia seen with AgNO3 alone. Treatment with interferon-gamma was found to restore decreased Ia expression in animals given AEF+AgNO3 but did not prevent amyloid A fibril deposition.
McGill Centre for the Study of Host Resistance, Montreal General Hospital Research Institute, Quebec, Canada.
C
C
Reid
L
L
Hébert
G
G
Pozzulo
F
F
Gervais
D000682:Amyloid; D000686:Amyloidosis / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D006023:Glycoproteins / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis; D016897:Respiratory Burst; D000685:Serum Amyloid A Protein / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000682:Amyloid; D000954:Antigens, Surface; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D000685:Serum Amyloid A Protein; C046748:amyloid enhancing factor; D013481:Superoxides