Stimulation of exocytotic membrane fusion by modified peptides of the rab3 effector domain: re-evaluation of the role of rab3 in regulated exocytosis.
We have shown previously that fusion between pancreatic zymogen granules and plasma membranes is stimulated by a peptide corresponding to the putative effector domain of rab3. Here we show that this stimulatory effect persists when the amino acid sequence of the peptide is substantially modified. We also show that an antibody raised against rab3a recognizes a protein of appropriate size on the zymogen-granule membrane, but has no effect on membrane fusion. We suggest that rab3 is not directly involved in the control of this membrane fusion event, and that the peptides are stimulating fusion by a mechanism unrelated to rab3.
MacLean
C M
CM
Department of Pharmacology, University of Cambridge, U.K.
Law
G J
GJ
Edwardson
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000502:physiology; D003594:Cytoplasmic Granules / Q000502:physiology; D005089:Exocytosis / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D008561:Membrane Fusion; D008969:Molecular Sequence Data; D010179:Pancreas / Q000648:ultrastructure*; D010446:Peptide Fragments / Q000737:chemistry / Q000502:physiology; D051381:Rats; D013329:Structure-Activity Relationship; D020692:rab3 GTP-Binding Proteins
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D019204:GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins