Peripheral-type benzodiazepine receptors and diazepam binding inhibitor-like immunoreactivity distribution in human peripheral blood mononuclear cells.
Peripheral-type benzodiazepine receptors (pBZr) in human lymphocytes have been detected only in mixtures of peripheral blood mononuclear cells (PBMC). The present investigation was designed to describe precisely the location of pBZr in the various sets and subsets of PBMC, purified using monoclonal antibodies to specific PBMC surface markers. Site densities and affinities of pBZr were measured in the intact cells by conventional binding, using 3H-PK 11195 as a ligand. Moreover, we used a specific radioimmunoassay to identify in these cells the presence of the polypeptide diazepam binding inhibitor (DBI), a putative endogenous ligand for various benzodiazepine receptors including the peripheral type. Two major findings are derived from these studies: first, the coexistence of pBZr and DBI, or closely related immunoreactive material, in all major lymphocyte sets and subsets, as well as in monocytes. And second, the significant correlation (r = 0.87, p < 0.001) observed between the density of pBZr in a given cell type and its abundance of DBI like-immunoreactivity (DBI-LI). For both pBZr and DBI-LI content the cell distribution was monocytes > B cells and large granular lymphocytes > T cells (CD3+ set or CD4+ and CD8+ subsets) (ANOVA: pBZr: F = 114.11, p < 0.001; DBI-LI: F = 20.79, p < 0.001). The results are discussed in terms of the possibility that DBI and pBZr might share a relevant interaction in immunocompetent elements, thereby contributing to a new route of connection between the immune and the nervous systems.
Department of Neurology, University of Turin, Italy.
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Rocca
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Bellone
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Benna
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Bergamasco
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Ravizza
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Ferrero
D001402:B-Lymphocytes / Q000378:metabolism; D002352:Carrier Proteins / Q000097:blood*; D026261:Diazepam Binding Inhibitor; D006801:Humans; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D011863:Radioimmunoassay; D011963:Receptors, GABA-A / Q000378:metabolism*
D002352:Carrier Proteins; D026261:Diazepam Binding Inhibitor; D007546:Isoquinolines; D011963:Receptors, GABA-A; C037850:PK 11195