Structural analysis on the sugar chains of human alpha 1-antitrypsin: presence of fucosylated biantennary glycan in hepatocellular carcinoma.
Chemical structures of the sugar chains of alpha 1-antitrypsin (AAT) from patients with hepatocellular carcinoma (HCC) and from healthy individuals with a different affinity for Lens culinaris agglutinin (LCA) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion in combination with reversed-phase and size-fractionation high-performance liquid chromatography. We found that the LCA-reactive species of AAT from patients with HCC carried both the biantennary sugar chain with a fucose residue at the innermost N-acetylglucosamine residue, Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc-PA, and the biantennary chain without a fucose residue, Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4GlcNAc-PA, at a ratio of about 1:0.6. The LCA-nonreactive species of AAT contained the biantennary sugar chain Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4GlcNAc as a major component. These results indicate that a characteristic feature of the carbohydrate chains of AAT from patients with HCC is an increment in fucosylation.
Department of Internal Medicine, Niigata University School of Medicine, Japan.
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Saitoh
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Aoyagi
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Asakura
D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005643:Fucose / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008113:Liver Neoplasms / Q000737:chemistry*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D011134:Polysaccharides / Q000032:analysis*; D000515:alpha 1-Antitrypsin / Q000737:chemistry* / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000378:metabolism
D002241:Carbohydrates; D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides; D000515:alpha 1-Antitrypsin; C033619:lentil lectin; D005643:Fucose; D005644:alpha-L-Fucosidase