Identification of genomic regions of the herpesvirus of turkeys (HVT) with helper activity for avian adeno-associated virus (AAAV).
Herpesvirus of turkeys (HVT) is a potent helper for the defective parvovirus avian adeno-associated virus (AAAV). To study the helper mechanism at the molecular level, we established a complete cosmid library of HVT DNA in a set of seven overlapping clones and transiently cotransfected secondary chicken embryo fibroblast (CEF) cells with AAAV DNA and recombinant cosmids (cBL) (individual as well as in different combinations). Using an AAAV-specific indirect immunofluorescence assay, we identified four regions on the HVT genome, represented by cBL267, cBL27, cBL33, and cBL34, which express helper functions for AAAV. As demonstrated by infection studies with extracts from cotransfected CEF cells, cBL267 promotes productive AAAV growth, while the helper effect induced by cBL27, cBL33, and cBL34 is limited to the synthesis of noninfectious AAAV antigen. In view of the data presented, possible HVT-specific helper mechanisms for AAAV are discussed.
Institut für Geflugelkrankheiten, Freie Universität Berlin, Federal Republic of Germany.
H J
HJ
Bauer
S
S
Schüller
G
G
Monreal
W
W
Lindenmaier
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D003360:Cosmids; D004279:DNA, Viral / Q000235:genetics; D000229:Dependovirus / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006378:Helper Viruses / Q000235:genetics / Q000502:physiology*; D006564:Herpesviridae / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014422:Turkeys
D004279:DNA, Viral