An adenovirus vector for gene transfer into neurons and glia in the brain.
The efficient introduction of genetic material into quiescent nerve cells is important in the study of brain function and for gene therapy of neurological disorders. A replication-deficient adenoviral vector that contained a reporter gene encoding beta-galactosidase infected rat nerve cells in vitro and in vivo. beta-Galactosidase was expressed in almost all sympathetic neurons and astrocytes in culture. After stereotactic inoculations into the rat hippocampus and the substantia nigra, beta-galactosidase activity was detected for 2 months. Infected cells were identified as microglial cells, astrocytes, or neurons with anatomical, morphological, and immunohistochemical criteria. No obvious cytopathic effect was observed.
Le Gal La Salle
G
G
Institut Alfred Fessard, Unité Propre de Recherche 2212, Centre National de la Recherche Scientifique (CNRS), Gif sur Yvette, France.
Robert
J J
JJ
Berrard
S
S
Ridoux
V
V
Stratford-Perricaudet
L D
LD
Perricaudet
M
M
Mallet
J
J
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000382:microbiology; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001921:Brain / Q000166:cytology*; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism* / Q000382:microbiology; D009474:Neurons / Q000378:metabolism* / Q000382:microbiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D013378:Substantia Nigra / Q000166:cytology / Q000378:metabolism; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics
D004247:DNA; D001616:beta-Galactosidase