[ATP formation in a model system with cytochrome c oxidase].
The ability of isolated cytochrome c-oxidase to form ATP is discovered. The process is accompanied by a decrease in inorganic phosphate and oxygen uptake, stimulated by reduced cytochrome c and inhibited by ammonium sulphate, uncoupler SF 6847 and oligomycin. In the ATP-free reaction mixture with cytochrome oxidase and orthophosphate the oxygen uptake is followed by pyrophosphate formation. In the absence of orthophosphate, cytochrome oxidase possesses the adenylate kinase activity which is stimulated by reduced cytochrome c, inhibited by oligomycin and ammonium sulphate and inactivated on heating.
Goncharova
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D000255:Adenosine Triphosphate / Q000032:analysis / Q000096:biosynthesis*; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008929:Mitochondria, Heart / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D010766:Phosphorylation; D051381:Rats
D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D000262:Adenylyl Cyclases