EPR spin-trapping study of nitric oxide formation during bilateral carotid occlusion in the rat.
The formation of nitric oxide (NO) radicals was demonstrated by electron paramagnetic resonance spectroscopy in the rat during varying degrees of brain ischemia. Diethyldithiocarbamate and Fe-citrate were used as in vivo spin-trapping reagents. The signal of NO spin adducts increased in accordance with the degree of ischemic insults. The formation of NO radicals was inhibited by NG-nitro-L-arginine methyl ester.
Sato
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Philadelphia Biomedical Research Institute, King of Prussia, PA 19406.
Tominaga
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Ohnishi
T
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Ohnishi
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ST
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D016893:Carotid Stenosis / Q000378:metabolism; D004050:Ditiocarb; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D005609:Free Radicals; D009569:Nitric Oxide; D004050:Ditiocarb