Decreased levels of glia-derived nexin/protease nexin I in irradiated rat spinal cord in vivo.
The pathophysiology of the cellular basis of radiation induced demyelination and white matter necrosis of the Central Nervous System (CNS) is poorly understood. There have been no previous studies that have shown the effect of irradiation on glia-derived nexin. In this study, rats were given cervical spinal cord irradiation, and glial derived nexin or protease nexin I (GDN/PNI) was measured on days 2, 7, 30, 60, 120, 130, and 145 after irradiation. The level of GDN/PNI significantly decreased after irradiation compared to levels in control spinal cord, and there was no detectable levels of GDN/PNI by the time paralysis developed. This study adds considerable weight to the proposal that GDN/PNI has an important role in the pathogenesis of CNS radiation damage.
Department of Neurosurgery, University of Texas M. D. Anderson Cancer Center, Houston 77030.
J S
JS
Rao
S
S
Kono
A
A
Rayford
K K
KK
Ang
Y
Y
Feng
D
D
Monard
R
R
Sawaya
D016564:Amyloid beta-Protein Precursor; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D009457:Neuroglia / Q000378:metabolism / Q000528:radiation effects*; D058255:Protease Nexins; D051381:Rats; D011956:Receptors, Cell Surface; D015843:Serpins / Q000378:metabolism* / Q000528:radiation effects; D013116:Spinal Cord / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors
D016564:Amyloid beta-Protein Precursor; D002352:Carrier Proteins; D058255:Protease Nexins; D011956:Receptors, Cell Surface; D015843:Serpins