Expression of synaptotagmin and syntaxin associated with N-type calcium channels in small cell lung cancer.
The presence of synaptic proteins involved in excitation/secretion coupling was examined in ten small cell lung cancer lines. N-Type calcium channels (omega-conotoxin receptors), synaptotagmin (p65) and syntaxin (HPC-1) were detected in eight. Co-immunoprecipitation experiments indicated that syntaxin can form a complex with synaptotagmin and calcium channels. The expression of synaptotagmin in small cell lung cancer may elicit an autoimmune response that reduces transmitter release at the nerve terminal.
David
P
P
INSERM U374, Faculté de Medecine Secteur Nord, Marseille, France.
el Far
O
O
Martin-Mouto
N
N
Poupon
M F
MF
Takahashi
M
M
Seagar
M J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis / Q000378:metabolism*; D015153:Blotting, Western; D015220:Calcium Channels / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002793:Cholic Acids; D006801:Humans; D007163:Immunosorbent Techniques; D008175:Lung Neoplasms / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D050863:Synaptotagmin I; D050857:Synaptotagmins; D050827:Syntaxin 1; D014407:Tumor Cells, Cultured; D020866:omega-Conotoxin GVIA
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015220:Calcium Channels; D002135:Calcium-Binding Proteins; D002793:Cholic Acids; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D010455:Peptides; C494212:STX1A protein, human; C494213:Stx1a protein, rat; D050863:Synaptotagmin I; D050827:Syntaxin 1; D050857:Synaptotagmins; D020866:omega-Conotoxin GVIA; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate