Calcium imaging.
We review herein the following questions related to Ca2+ imaging: what are the key elements to consider in a Ca2+ imaging system? What are the limits of such systems? What are the main artifacts and to what specific applications are a Ca2+ imaging systems best adapted?
Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris, France.
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A
Trautmann
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YP
Tan
D016477:Artifacts; D002118:Calcium / Q000032:analysis*; D002477:Cells / Q000737:chemistry / Q000648:ultrastructure; D003116:Color; D005456:Fluorescent Dyes / Q000032:analysis*; D007091:Image Processing, Computer-Assisted; D008213:Lymphocyte Activation; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D009862:Online Systems; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure
D005456:Fluorescent Dyes; D002118:Calcium