M13 and pUC vectors with new unique restriction sites for cloning.
Several vectors based on the widely used phage M13 and plasmid pUC were constructed. The vectors contain polylinkers (MCS) for DNA insertions with several new restriction sites (e.g., ApaI, NotI, StuI, SacII). Moreover, the NarI site in the nonessential part of the vector molecule was changed into a BssHII site, so that the NarI site in the MCS became unique.
Institute of Molecular Genetics, Czech Academy of Sciences, Prague.
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Benes
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Hostomský
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Arnold
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Paces
D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes; C044171:endodeoxyribonuclease BSSHII; D015252:Deoxyribonucleases, Type II Site-Specific; C046719:GGCGCC-specific type II deoxyribonucleases