Calcium/calmodulin-stimulated protein kinase II is present in primary cultures of cerebral endothelial cells.
Calcium/calmodulin-stimulated protein kinase II (CaM-PK II), a major kinase in brain, has been established to play an important role in neurotransmitter release and organization of postsynaptic receptors, and it is known to be involved in long-term potentiation and memory. Less is known about the function of this enzyme in nonneural cells. Here we report on the production, presence, and phosphorylation of the alpha-subunit of CaM-PK II in primary cultures of cerebral endothelial cells. These results raise the possibility that alpha-CaM-PK II can act as one of the key enzymes of calcium-mediated intracellular signaling in the cerebral endothelial cells and suggest that alpha-CaM-PK II may participate in such basic cellular processes as permeability in physiological and pathological conditions.
Deli
M A
MA
Laboratory of Molecular Neurobiology, Biological Research Center, Szeged, Hungary.
Joó
F
F
Krizbai
I
I
Lengyel
I
I
Nunzi
M G
MG
Wolff
J R
JR
D000818:Animals; D015153:Blotting, Western; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*
D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases