Structural similarities between M. luteus and E. coli DNA topoisomerase I.
Type I DNA topoisomerase has been isolated from Micrococcus luteus following a procedure that takes advantage of the binding of the enzyme to heparin and single stranded DNA. Almost pure enzyme was obtained using two successive affinity chromatography steps: on heparin Sepharose and ssDNA cellulose. The method was rapid and allowed the isolation of DNA topoisomerase I from M. luteus in mg quantities. Polyclonal antibodies raised in rabbit were shown to cross-react with type I DNA topoisomerase of Escherichia coli. Partial protein sequencing of the M. luteus enzyme identified a peptide with high similarity to the putative protein sequence of E. coli DNA topoisomerase I, aligning with 73% identity to amino acids 491-505.
Anderluzzi
D
D
Research Laboratories, GLAXO S.p.A., Verona, Italy.
Pedrini
A M
AM
D000595:Amino Acid Sequence; D001483:Base Sequence; D004264:DNA Topoisomerases, Type I / Q000737:chemistry* / Q000276:immunology; D004926:Escherichia coli / Q000201:enzymology*; D016982:Micrococcus luteus / Q000201:enzymology*; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
D009841:Oligonucleotides; D004264:DNA Topoisomerases, Type I