Molecular cloning and characterization of mouse TIE and TEK receptor tyrosine kinase genes and their expression in hematopoietic stem cells.
To identify receptor tyrosine kinases (RTKs) critical to early hematopoiesis, we performed polymerase chain reaction-based cloning from yolk sac and highly enriched bone marrow hematopoietic stem cells (HSCs). Characterization of two novel genes of their full-length cDNA sequences revealed that they were mouse homologues of the endothelial cell RTK genes, TIE and TEK. They shared a unique structural property of coexistent immunoglobulin-like domain, epidermal growth factor-like repeats, and fibronectin type III repeats in their extracellular domains. Both genes were expressed in a similar fashion in adult tissues and primitive hematopoietic cells, predominantly in the bone marrow HSCs.
Department of Cell Differentiation, Kumamoto University School of Medicine, Japan.
A
A
Iwama
I
I
Hamaguchi
M
M
Hashiyama
Y
Y
Murayama
K
K
Yasunaga
T
T
Suda
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001853:Bone Marrow / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D017124:Conserved Sequence; D004815:Epidermal Growth Factor / Q000235:genetics; D005333:Fetus; D015723:Gene Library; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007942:Leukemia, Experimental; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D042764:Receptors, TIE; D017386:Sequence Homology, Amino Acid; D015017:Yolk Sac / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D011505:Protein-Tyrosine Kinases; D042764:Receptors, TIE