In vitro HPV-11 infection of human foreskin.
Study of the infectious process of human papillomavirus type 11 (HPV-11) has been facilitated by the discovery that HPV-11-infected neonatal human foreskin epithelium can proliferate as xenografts into condyloma-like growths within athymic nude mice. Here we describe detection of HPV-11 infection of neonatal human foreskin-derived keratinocytes, infected and cultured entirely in vitro, by use of the polymerase chain reaction and primers straddling the splice donor/acceptor site of the most prevalent early gene HPV-11 transcript (E1 increase E4). Expression of the E1 increase E4 HPV-11 mRNA is abrogated by 60 degrees C heat inactivation of the inoculum. HPV-11-infected foreskin explants continue to produce the E1 increase E4 mRNA for up to 5 weeks in culture, and second-passage keratinocytes derived from infected explant outgrowths continue to produce the E1 increase E4 mRNA. The in vitro system described here provides a new way to study HPV-11 infection and may be useful in evaluating early events of infection.
Department of Obstetrics and Gynecology, University of California at Davis, School of Medicine 95816.
L H
LH
Smith
C
C
Foster
M E
ME
Hitchcock
R
R
Isseroff
D001483:Base Sequence; D015152:Blotting, Northern; D046508:Culture Techniques; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D003872:Dermatitis / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D015603:Keratinocytes / Q000382:microbiology; D008297:Male; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D014412:Tumor Virus Infections
D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA