[Changes in cytokine levels in ascitic fluid after intraperitoneal administration of OK-432].
In order to evaluate the biological response after intraperitoneal administration of OK-432, we studied the changes of cytokine levels in ascitic fluid. In 6 advanced gastric cancer patients with peritoneal dissemination or extensive lymph node metastases, OK-432 20 KE was administered intraperitoneally during operation and the IL-6, IL-8 and IFN-gamma levels in ascitic fluid were measured from 0 to 5 postoperative days. These results were compared with those obtained from non-OK-432 administered control groups (17 cases). The ascitic level of IL-8 increased immediately after operation and gradually decreased. In the OK-432 treated group, the elevation of IL-8 on 0 and 1 postoperative days was remarkable, and there were statistically significant differences with the control group. Similarly, the ascitic level of IL-6 elevated soon after operation and then decreased gradually. In the OK-432 treated group, the ascitic level of IL-6 was significantly higher than in the control group after 3 postoperative days. There were no differences in changes of ascitic IFN-gamma levels between the groups. From these results, IL-6 and IL-8 appeared to be induced in ascitic fluid by intraperitoneal administration of OK-432.
Dept. of Surgery 1, Iwate Medical University.
M
M
Terashima
A
A
Takagane
K
K
Abe
M
M
Kuboi
H
H
Asahi
K
K
Saito
D001202:Ascitic Fluid / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007263:Infusions, Parenteral; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008207:Lymphatic Metastasis; D010844:Picibanil / Q000008:administration & dosage*; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy
D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D010844:Picibanil; D007371:Interferon-gamma