Altered cytokine activity in adjuvant inhibition of autoimmune diabetes.
The destructive insulitis that develops in disease-prone NOD mice is characterized by a high frequency of IFN-gamma-producing cells in the lesion. Complete Freund's adjuvant (CFA) challenge of young, disease-prone animals inhibits the development of diabetes but does not alter the frequency with which pancreatic insulitis develops. However, the non-destructive lesions that develop in syngeneic islets transplanted to the kidney capsule of NOD mice following CFA therapy differ in cytokine production from the destructive lesions that develop in control animals transplanted with NOD islets. Non-destructive lesions are characterized by a high frequency of IL4-producing cells and a relatively low frequency of IFN-gamma-producing cells.
Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver 80262.
N N
NN
Shehadeh
F
F
LaRosa
K J
KJ
Lafferty
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D005620:Freund's Adjuvant; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000276:immunology; D016381:Islets of Langerhans Transplantation / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D016688:Mice, Inbred NOD; D010195:Pancreatitis / Q000276:immunology; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1
D015847:Interleukin-4; D007371:Interferon-gamma; D005620:Freund's Adjuvant; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1