Species-specific oligonucleotide probes complementary to 16S rRNA of Mycoplasma gallisepticum and Mycoplasma synoviae.
Mycoplasma gallisepticum and M synoviae are important avian pathogens causing respiratory diseases which result in great economic losses in poultry farming. Two oligonucleotide probes, complementary to the variable region V8 of 16S rRNA from the avian mycoplasmas M gallisepticum and M synoviae have, therefore, been designed and used in direct filter hybridisation experiments. Both probes gave strong hybridisation signals with their homologous targets, whereas no cross-hybridisations were obtained with any of the other avian mycoplasmas tested. It was possible to detect 2-3 x 10(4) mycoplasma organisms by direct filter hybridisation experiments with radiolabelled probes. The probes were also used to analyse several laboratory strains and field isolates of M gallisepticum and M synoviae with complete agreement between the probe technique and the other methods used for species determination. Atypical M gallisepticum strains also gave strong hybridisation signals with the M gallisepticum specific probe.
National Veterinary Institute, Uppsala, Sweden.
C
C
Fernández
J G
JG
Mattsson
G
G
Bölske
S
S
Levisohn
K E
KE
Johansson
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004275:DNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D015345:Oligonucleotide Probes; D011201:Poultry Diseases / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014422:Turkeys
D004275:DNA, Ribosomal; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S