Effect of a capsid-stabilizing pyridazinamine, R 78206, on the eclipse and intracellular location of poliovirus.
R 78206 (a pyridazinamine derivative) inhibits the formation of poliovirus eclipse particles. Its effect on the intracellular location of poliovirus was studied by separating subcellular fractions in iso-osmotic Nycodenz gradients. The compound did not inhibit internalization of intact virus into small lipid vesicles, but it did inhibit the release of virus from these vesicles and its entry into lysosomes.
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
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Ofori-Anyinam
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Vrijsen
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Kronenberger
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Boeyé
D000998:Antiviral Agents / Q000494:pharmacology*; D002451:Cell Compartmentation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D010880:Piperidines / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects* / Q000378:metabolism; D011724:Pyridazines / Q000494:pharmacology*; D012367:RNA, Viral / Q000378:metabolism; D014771:Virion / Q000378:metabolism
D000998:Antiviral Agents; D010880:Piperidines; D011724:Pyridazines; D012367:RNA, Viral; C070921:R 78206