Stimulation of rat liver microsomal cGMP-inhibited cAMP phosphodiesterase (PDE III) by phospholipase C and D.
The specific activity of cGMP-inhibited cAMP phosphodiesterase (PDE III) of rat liver microsomal membranes is increased in a concentration-dependent way by adding phospholipase C from Clostridium perfringens or phospholipase D from Streptomyces chromofuscus. The effect depends on an increase in Vmax of the enzyme. Treatment of microsomal membranes with phospholipase C causes a marked increase in the relative amounts of phosphatidylserine and phosphatidylinositol, and mild stimulation of PDE III activity. Treatment with phospholipase D increases phosphatidic acid and strongly increases PDE III activity. These data suggest that phosphatidic acid is the most important regulator of membrane-bound PDE III activity in liver.
Department of Pharmacology, University of Padova, Italy.
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F
Carpenedo
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M
Floreani
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D000818:Animals; D003016:Clostridium perfringens / Q000201:enzymology; D006152:Cyclic GMP / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology* / Q000648:ultrastructure; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D010739:Phospholipase D / Q000494:pharmacology*; D051381:Rats; D013302:Streptomyces / Q000201:enzymology; D010738:Type C Phospholipases / Q000494:pharmacology*
D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010738:Type C Phospholipases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D010739:Phospholipase D; D006152:Cyclic GMP