A rapid quantitative method for detecting infectious bursal disease virus using polystyrene latex microspheres.
A monoclonal antibody (mAb) to infectious bursal disease virus (IBDV) was bound to polystyrene latex microspheres. The microspheres agglutinated with extracts of bursae and sera from chickens infected with all strains or isolates of IBDV tested. Agglutination appeared within a 10-min reaction time. The assay could detect a 10(3.7) to 10(4.5) mean embryo infective dose (EID50) of the virus in 0.01 ml and the titer of the assay was 10- to 40-times higher than that of the agar gel precipitin test.
Nakamura
T
T
Nippon Institute for Biological Science, Tokyo, Japan.
Kato
A
A
Lin
Z
Z
Hiraga
M
M
Nunoya
T
T
Otaki
Y
Y
Ueda
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis; D002060:Bursa of Fabricius / Q000382:microbiology; D002642:Chick Embryo / Q000382:microbiology; D002645:Chickens / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D007243:Infectious bursal disease virus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D007841:Latex Fixation Tests; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008863:Microspheres; D011201:Poultry Diseases / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D011233:Precipitin Tests; D012088:Reoviridae Infections / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014765:Viral Vaccines; D014766:Viremia / Q000382:microbiology / Q000662:veterinary
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014765:Viral Vaccines