Characterization of a monoclonal antibody against a nuclear antigen associated with serotype-1 Marek's disease virus-infected and transformed cells.
A monoclonal antibody (MAb), 2BN90, was characterized on a panel of Marek's disease virus (MDV) isolates representing three antigenically related serotypes and lymphoblastoid cell lines established from Marek's disease (MD) tumors. It was reactive with all serotype 1 isolates, including CV1988, in both enzyme-linked immunosorbent assay (ELISA) and immunofluorescence (IF) test. The MAb immunoprecipitated and immunoblotted a 40-kilodalton protein, which was found to be phosphorylated by metabolic labeling with 32P inorganic phosphate, and localized to the cell nucleus using the IF test. It reacted with MDV-transformed lymphoblastoid cell lines, and the percentage of reactive cells was enhanced after treatment with iododeoxyuridine. MAb 2BN90 may be useful as a type-specific antibody of choice for serotyping MDV serotype 1 strains and as a probe for studying the role of pp40 in MDV infection and transformation.
USDA-Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002461:Cell Line, Transformed; D002645:Chickens; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D016399:Lymphoma, T-Cell; D014407:Tumor Cells, Cultured / Q000382:microbiology; D014422:Turkeys
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral