p60v-src causes tyrosine phosphorylation and inactivation of the N-cadherin-catenin cell adhesion system.
Transformation of chick embryonic fibroblasts with Rous sarcoma virus strongly suppresses N-cadherin-mediated cell-cell adhesion, without inhibiting its expression. This suppression is correlated with tyrosine phosphorylation of N-cadherin and catenins, the cadherin-associated proteins, which are known to regulate cadherin function. Experiments with non-myristylation and temperature-sensitive mutants of RSV and with herbimycin A, a potent inhibitor of tyrosine kinases, suggest that both the suppression of cell adhesion and tyrosine phosphorylation of catenins are highly transformation-specific.
Hamaguchi
M
M
Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
Matsuyoshi
N
N
Ohnishi
Y
Y
Gotoh
B
B
Takeichi
M
M
Nagai
Y
Y
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D016227:Benzoquinones; D015820:Cadherins / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002449:Cell Aggregation; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D003598:Cytoskeletal Proteins / Q000502:physiology*; D005347:Fibroblasts; D047029:Lactams, Macrocyclic; D008715:Methionine / Q000378:metabolism; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology; D017828:Rifabutin / Q000031:analogs & derivatives; D014443:Tyrosine
D016227:Benzoquinones; D015820:Cadherins; D003598:Cytoskeletal Proteins; D047029:Lactams, Macrocyclic; D010710:Phosphates; D011809:Quinones; D017828:Rifabutin; D014443:Tyrosine; C020754:herbimycin; D008715:Methionine; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)