Leukotriene B4 and C4 generation by human colostral macrophages in vitro.
To investigate leukotriene (LT) generation by human colostral macrophages (CM phi), we evaluated calcium ionophore A23187-induced LTB4 and LTC4 production by CM phi obtained from mothers who had had preterm and term deliveries. As a comparison, we also investigated such production of these LTs by blood monocytes (BMo). LTB4 and LTC4 production by preterm CM phi did not significantly differ from that by term CM phi. LTB4 and LTC4 production by both preterm and term CM phi was significantly lower than that by BMo. The ratio of LTC4 to LTB4 did not differ significantly between preterm and term CM phi. The CM phi ratios were significantly lower than that of BMo. These findings suggest that human CM phi can generate LTB4 and LTC4 but that production, especially LTC4 production, is suppressed compared with that of BMo.
Department of Paediatrics, University of Göteborg, Sweden.
T
T
Shimizu
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A
Niklasson
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B
Strandvik
D001773:Blood Cells / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D003126:Colostrum / Q000166:cytology / Q000378:metabolism*; D036861:Delivery, Obstetric; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D011247:Pregnancy; D013189:SRS-A / Q000096:biosynthesis*
D013189:SRS-A; D007975:Leukotriene B4; D000001:Calcimycin