Reduction of proteolytic degradation by chlorhexidine.
The present investigation was designed to evaluate the effect of the antimicrobial agent chlorhexidine on proteolytic degradation. Chlorhexidine, at a final concentration of 0.01%, significantly (p = 0.05) affected the degradation of the general chromogenic substrate azocoll by both commercial proteases and cell-bound bacterial proteases. Reduction of proteolytic degradation was observed following pre-treatment of either azocoll or bacterial cells with chlorhexidine. Chlorhexidine was also found to prevent degradation of type I collagen by Porphyromonas gingivalis cells. The mechanism of inhibition of proteolytic degradation appears to be related to an electrostatic chlorhexidine-protein interaction.
Grenier
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Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Ste-Foy, Québec, Canada.
D001391:Azo Compounds / Q000378:metabolism; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors; D002710:Chlorhexidine / Q000494:pharmacology*; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D003094:Collagen / Q000378:metabolism; D004396:Coloring Agents; D019286:Endopeptidase K; D010450:Endopeptidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000037:antagonists & inhibitors; D016966:Porphyromonas gingivalis / Q000201:enzymology; D011480:Protease Inhibitors / Q000494:pharmacology*; D012697:Serine Endopeptidases / Q000378:metabolism; D014208:Treponema / Q000201:enzymology; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000494:pharmacology
D001391:Azo Compounds; D001426:Bacterial Proteins; D004396:Coloring Agents; D008565:Membrane Proteins; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; C028470:Azocoll; D003094:Collagen; D010450:Endopeptidases; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D019286:Endopeptidase K; D002710:Chlorhexidine