Nucleotide sequence of bovine preprocathepsin B. A study of polymorphism in the protein coding region.
A cDNA encoding bovine procathepsin B was isolated. The deduced amino acid sequence revealed that a stop (TAG) codon, instead of a Trp-257 codon (TGG), generates in bovine a cathepsin B precursor four amino acids shorter than in other species. Because micro-heterogeneities were previously reported in the cathepsin B primary structure, sequence polymorphism in the protein coding region was then investigated by PCR sequencing of genomic fragments and RNase protection assays. Experiments performed with 12-15 animals of three breeds did not reveal any difference with our cDNA sequence. We conclude that sequence polymorphism in bovine cathepsin B is a rare event, and can only result from the expression of different alleles of a unique gene.
Unité de Recherches sur l'Expression des Protéases, Institut National de la Recherche Agronomique, Ceyrat, France.
S
S
Mordier
D
D
Béchet
M P
MP
Roux
A
A
Obled
M
M
Ferrara
D000818:Animals; D001483:Base Sequence; D002401:Cathepsin B / Q000235:genetics*; D002403:Cathepsins / Q000235:genetics*; D002417:Cattle; D004247:DNA / Q000032:analysis*; D004792:Enzyme Precursors / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012260:Ribonucleases; D016415:Sequence Alignment
D004792:Enzyme Precursors; D004247:DNA; D012260:Ribonucleases; D002403:Cathepsins; C057831:preprocathepsin B; D002401:Cathepsin B