Exhaustive exercise affects fluidity and alpha-tocopherol levels in brain synaptosomal membranes of normal and vitamin E supplemented rats.
Alpha-tocopherol level and fluidity were studied in the neuronal membrane of rat brain after exhaustive exercise. The order parameter, 5-doxyl-stearic acid (5-DS), which is utilized for assessing the fluidity of the lipid bilayer closer to the hydrophilic face of the membrane, decreased in the pons-medulla oblongata, and the motion parameter, 16-doxyl-stearic acid (16-DS) for the core of the lipid bilayer, decreased in the cortex, hippocampus, hypothalamus and striatum, whereas it increased in the cerebellum after exercise. The w/s ratio of n-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-maleimido (maleimido-TEMPO) for the conformation of SH-protein also decreased in the hippocampus and midbrain after exercise. These changes were not observed in alpha-tocopheryl acetate supplemented rats after exercise. Although the levels of 5-DS, 16-DS and maleimido-TEMPO were affected by alpha-tocopheryl acetate in rat neuronal membranes, fluidity changes were reversible with exercise.
Division of Life Support Technology, Yamagata Technopolis Foundation.
M
M
Hiramatsu
R
R
Edamatsu
R D
RD
Velasco
S
S
Ooba
K
K
Kanakura
A
A
Mori
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D007425:Intracellular Membranes / Q000378:metabolism; D008560:Membrane Fluidity / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013113:Spin Labels; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure; D014810:Vitamin E / Q000378:metabolism* / Q000494:pharmacology*
D003497:Cyclic N-Oxides; D013113:Spin Labels; D014810:Vitamin E; C005072:5-doxylstearic acid; C032363:16-nitroxystearic acid; C003959:TEMPO