Rat liver paraoxonase: subcellular distribution and characterization.
The subcellular localization and some biochemical properties of rat liver paraoxonase have been studied in order to establish a correlation with plasma enzyme. The whole paraoxonase activity was found in the microsomal fraction. Rat plasma and liver paraoxonase showed similar optimum pH (8.5), Km (0.4 mM) and calcium requirement, but differed in the response to several inhibitors.
Department of Legal Medicine, Faculty of Medicine, University Hospital, University of Granada, Spain.
F
F
Gil
A
A
Pla
M C
MC
Gonzalvo
A F
AF
Hernández
E
E
Villanueva
D000818:Animals; D043303:Aryldialkylphosphatase; D002118:Calcium / Q000494:pharmacology; D002498:Centrifugation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D010261:Paraoxon / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000201:enzymology; D013395:Sucrose / Q000494:pharmacology
D013395:Sucrose; D010744:Phosphoric Monoester Hydrolases; D043303:Aryldialkylphosphatase; D010261:Paraoxon; D002118:Calcium