Leukocyte depletion in a neonatal model of cardiac surgery.
Neonatal cardiac surgical procedures continue to be associated with a considerable incidence of severe post-operative ventricular dysfunction. The role of neutrophils in mediating such injury has recently been proposed but remains controversial. The present study was undertaken to examine the potential benefits of leukocyte depletion for myocardial preservation using an in situ, in vivo porcine model of neonatal cardiac surgery. Sixteen 3- to 5-day-old piglets, 8 controls and 8 leukocyte-depleted animals (LD group), underwent 90 minutes of hypothermic ischemia. Mechanical leukocyte filtration during cardiopulmonary bypass reduced the granulocyte count in the initial reperfusate to 0.7% of controls. This was associated with a reduction in leukocyte sequestration in the coronary vascular bed (p < 0.005), a decrease in myocardial creatine kinase release (p < 0.02), and a reduction in coronary vascular resistance (p < 0.03). These changes in physiological response to ischemia were associated with improved postischemic recovery of left ventricular systolic function in LD animals (p < 0.05), although there was no significant improvement in diastolic function. Application of this technique in neonatal cardiac operations may improve myocardial protection and reduce the associated morbidity and mortality.
Division of Cardiac Surgery, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
I C
IC
Wilson
J M
JM
DiNatale
A M
AM
Gillinov
W E
WE
Curtis
D E
DE
Cameron
T J
TJ
Gardner
D000818:Animals; D000831:Animals, Newborn; D001706:Biopsy; D001810:Blood Volume / Q000502:physiology; D002315:Cardiopulmonary Bypass; D003326:Coronary Circulation / Q000502:physiology; D005078:Exchange Transfusion, Whole Blood; D006098:Granulocytes / Q000502:physiology; D006344:Heart Septal Defects, Atrial / Q000503:physiopathology / Q000601:surgery*; D006439:Hemodynamics / Q000502:physiology*; D007937:Leukapheresis / Q000295:instrumentation*; D007958:Leukocyte Count; D017202:Myocardial Ischemia / Q000503:physiopathology*; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D009206:Myocardium / Q000473:pathology; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000502:physiology; D011652:Pulmonary Circulation / Q000502:physiology; D013552:Swine; D014655:Vascular Resistance / Q000502:physiology; D016277:Ventricular Function, Left / Q000502:physiology
D009195:Peroxidase