Numerical and functional modifications in platelets induced by polyester coated by a hydrophilic polymer.
We evaluated the alterations in number, functionality and release reaction of the platelets contained in plasma, filtered through a polyester filter and coated with a hydrophilic polymer. The alterations in number were examined by counting before and after filtration. The morphological modifications were studied by determining the mean platelet volume. The functional alterations were analysed by a platelet aggregation test, induced by ADP and collagen. The presence of products from the release reaction in filtered plasma was studied using radioimmunoassays of beta-thromboglobulin, platelet factor 4 and thromboxane B2. The results obtained showed that the filtration of plasma through the material did not determine a significant platelet adhesion, did not alter the volume or the functionality of the platelets and induced no release reaction.
Laboratory for Biocompatibility Research on Implant Materials, Instituti Ortopedici Rizzoli, Bologna, Italy.
E
E
Cenni
D
D
Cavedagna
G
G
Falsone
G
G
Mari
A
A
Pizzoferrato
D001672:Biocompatible Materials; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology*; D006440:Hemofiltration / Q000379:methods*; D006801:Humans; D015539:Platelet Activation / Q000502:physiology; D010974:Platelet Aggregation / Q000502:physiology; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000032:analysis / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D013929:Thromboxane B2 / Q000097:blood; D001620:beta-Thromboglobulin / Q000032:analysis / Q000378:metabolism
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D001620:beta-Thromboglobulin; D010978:Platelet Factor 4; D013929:Thromboxane B2