Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe.
A nucleic acid hybridization assay was developed to rapidly detect small quantities of aquareovirus RNAs in infected cells and organs. Cloned cDNA copies were synthesized from the genomic RNA of the SBR strain of aquareovirus. By using cloned cDNA probes, aquareovirus RNAs were detected in spleen and kidney tissues of experimentally infected fish.
Subramanian
K
K
College of Veterinary Medicine, University of Maryland, College Park 20742.
Lupiani
B
B
Hetrick
F M
FM
Samal
S K
SK
D000818:Animals; D003001:Cloning, Molecular; D015342:DNA Probes; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology; D005399:Fishes / Q000382:microbiology*; D007668:Kidney / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D012087:Reoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D013154:Spleen / Q000382:microbiology; D014337:Trout / Q000382:microbiology
D015342:DNA Probes; D012367:RNA, Viral