Viral inactivation of vesicular stomatitis virus in normal human serum by cross-linked polyvinylpyrrolidone.
Methods for inactivating virus contaminants in serum, cryoprecipitate-poor plasma, and protein concentrates need to be identified. In this study, vesicular stomatitis virus (VSV)-spiked human serum and cryoprecipitate-poor plasma were treated with cross-linked povidone iodine (XLPVPI) at concentrations of 0, 4, 6, 8, and 10 mg/mL up to 120 min at 4 and 24 degrees C. The activities of virus and relevant proteins were examined. The results indicated that XLPVPI at concentrations that inactivate > 5 logs of VSV in serum decreased factor IX and protein C activities by < 10% in cryoprecipitate-poor plasma. At concentrations up to 10 mg of XLPVPI/mL, < 10% of protein C and factor IX activity was lost after incubation for 5 min at 24 degrees C. In addition, < 10% loss in protein C and factor IX activity was observed at 4 degrees C after treatment with < or = 6 mg of XLPVPI/mL for 20 min. Treatment of human serum with 6 mg of XLPVPI/mL at 4 degrees C and 8 mg of XLPVPI/mL at 24 degrees C for 5 min provided inactivation of > 5 logs of VSV.
Holland Laboratory, American Red Cross, Plasma Derivatives Department, Rockville, MD 20855.
F A
FA
Highsmith
M
M
Caple
B
B
Walthall
E
E
Shanbrom
W N
WN
Drohan
D001769:Blood / Q000382:microbiology*; D003432:Cross-Linking Reagents; D005164:Factor IX / Q000378:metabolism; D006801:Humans; D011205:Povidone / Q000494:pharmacology*; D011206:Povidone-Iodine / Q000494:pharmacology; D011486:Protein C / Q000378:metabolism; D013696:Temperature; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*
D003432:Cross-Linking Reagents; D011486:Protein C; D011206:Povidone-Iodine; D005164:Factor IX; D011205:Povidone