Preparations of Rp-cyclic adenosine 3',5'-phosphorothioate (Rp-cAMPS) can contain biologically active amounts of adenosine.
Superoxide anion (O2-.) production from human neutrophils stimulated by N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP, 1 microM) was inhibited by preparations of the inhibitor of cAMP-dependent protein kinase, Rp-cyclic adenosine 3',5'-phosphorothioate (Rp-cAMPS, 100 microM). This effect of Rp-cAMPS was reversed by xanthine amine congener (0.1 microM), an adenosine receptor antagonist, and by low concentrations of adenosine desaminase (0.02 mg/ml). HPLC analysis shows that these preparations of Rp-cAMPS contained concentrations of adenosine which could produce significant inhibition of fMLP-induced O2-. production. These results suggest that Rp-cAMPS should be used with caution in cells or tissues containing adenosine receptors, and that preparations of Rp-cAMPS should be treated with adenosine desaminase before use to avoid activation of adenosine receptors.
Musgrave
I F
IF
Institut für Pharmakologie, Freie Universität Berlin, Germany.
Genieser
H G
HG
Maronde
E
E
Seifert
R
R
D000241:Adenosine / Q000032:analysis*; D000243:Adenosine Deaminase / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D047428:Protein Kinase Inhibitors; D013481:Superoxides / Q000097:blood; D013873:Thionucleotides / Q000032:analysis* / Q000494:pharmacology
D047428:Protein Kinase Inhibitors; D013873:Thionucleotides; D013481:Superoxides; C016957:adenosine-3',5'-cyclic phosphorothioate; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000242:Cyclic AMP; D000243:Adenosine Deaminase; D000241:Adenosine