Prevalence of HPV in a Melbourne female STD population: comparison of RNA and DNA probes in detecting HPV by dot blot hybridization.
A total of 377 women, consecutively selected as first attenders to a sexually transmitted diseases clinic in Melbourne, Australia, were examined for overt Condylomata acuminata and were screened for genital HPV DNA types 6, 11, 16, 18, 31, 33 and (35) using 2 dot blot hybridization methods. Overall, there was a 90% positivity correlation between the 2 methods with HPV DNA being detected in 12% of ectocervical samples. Overt warts were found in 15% of the women and HPV DNA was detected at the cervix in 35% with cytology predicting HPV with or without dysplasia in 27%. Thirteen percent had a past history of warts but none on examination and HPV DNA was evident in 16% while 18% had cytological features of HPV. Those with no warts evident and no past history of warts had both HPV DNA and cytological features of HPV in 7%.
Department of Microbiology, Royal Women's Hospital, Carlton, Victoria, Australia.
A J
AJ
Borg
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G
Medley
S M
SM
Garland
D003218:Condylomata Acuminata / Q000175:diagnosis / Q000453:epidemiology*; D015344:DNA Probes, HPV / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D005831:Genital Diseases, Female / Q000175:diagnosis / Q000453:epidemiology*; D006801:Humans; D015151:Immunoblotting; D008403:Mass Screening; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D015347:RNA Probes; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000453:epidemiology*; D014739:Victoria / Q000453:epidemiology
D015344:DNA Probes, HPV; D004279:DNA, Viral; D015347:RNA Probes