Gross and microvascular distribution of retrograde cardioplegia in explanted human hearts.
In this report, explanted hearts from transplant recipients with the diagnosis of idiopathic cardiomyopathy underwent a blood cardioplegia arrest and extended subatrial resection to preserve their coronary sinus venous system. The coronary sinus and left and right coronary arteries were then cannulated and warm blood cardioplegia retrograde infused at a pressure of 30 to 40 mm Hg. Effluent from the coronary arteries and thebesian veins was then collected. Hearts were subsequently fixed with retrograde glutaraldehyde perfusion and perfused retrograde with NTB-2 (an inert intracapillary marker). Histologic sections were examined from 12 separate sites. There was no significant difference in the percentage of capillaries perfused by retrograde-delivered cardioplegia between corresponding regions of the left and right ventricles. However, effluent analysis indicated that 67.2% +/- 6.4% of retrograde-delivered blood cardioplegia was shunted through thebesian veins, thereby bypassing the microvasculature, whereas 29.3% +/- 6.3% and 3.5% +/- 3.1% traversed the myocardium supplied by the left and right coronary arteries, respectively. The results indicate that all regions of both ventricles are perfused by retrograde blood cardioplegia. However, they also suggest that nutrient flow to the microvasculature of the right ventricle is minimal during retrograde cardioplegia.
Gates
R N
RN
Department of Surgery, University of California, Los Angeles, Medical Center 90024.
Laks
H
H
Drinkwater
D C
DC
Pearl
J M
JM
Zaragoza
A M
AM
Lewis
W
W
Sorensen
T J
TJ
Kaczer
E M
EM
Chang
P A
PA
D001769:Blood; D002311:Cardiomyopathy, Dilated / Q000473:pathology; D002314:Cardioplegic Solutions; D003331:Coronary Vessels / Q000033:anatomy & histology; D006321:Heart / Q000033:anatomy & histology*; D006324:Heart Arrest, Induced / Q000379:methods*; D006801:Humans; D008833:Microcirculation / Q000033:anatomy & histology; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology; D010477:Perfusion
D002314:Cardioplegic Solutions