Pertussis toxin-induced redistribution of cortical actomyosin and inhibition of phagocytosis in rat Kupffer cells.
The mechanism of phagocytosis by Kupffer cells has been shown to be related to the Ca(2+)-calmodulin and the actomyosin systems. However the role of the transmembrane signal transmitter, G-protein, is still unknown. In this study, a quantitative evaluation of phagocytosis by Kupffer cells of rats in culture and the effects of pertussis toxin, a G-protein inhibitor, on the phagocytic function and morphology of Kupffer cells were investigated. Pertussis toxin inhibited phagocytosis of Kupffer cells with dysfunction of the actomyosin system. The inhibitory effects of pertussis toxin suggest that G-protein may be involved in the mechanism of transmembrane signalling in phagocytosis by Kupffer cells.
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
M
M
Hirose
S
S
Watanabe
T
T
Ueno
N
N
Kitami
N
N
Sato
D000199:Actins / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000502:physiology; D066298:In Vitro Techniques; D007728:Kupffer Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009218:Myosins / Q000378:metabolism; D037342:Pertussis Toxin; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D014746:Vimentin / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D000199:Actins; D014746:Vimentin; D010566:Virulence Factors, Bordetella; D000205:Actomyosin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D009218:Myosins