Production of interleukin 1 by rat pleural leucocytes in culture after intratracheal instillation of crocidolite asbestos.
This study was undertaken to investigate the production of interleukin 1 (IL-1) by pleural leucocytes in culture and to evaluate the influence of intratracheal instillation of crocidolite asbestos on this production. Normal pleural leucocytes spontaneously released IL-1 in culture and stimulation with lipopolysaccharide (LPS) dramatically increased production. Intratracheal instillation with crocidolite asbestos induced recruitment of pleural leucocytes in the longer term and changed IL-1 production by the leucocytes. Reduced production of IL-1 was found by one day after instillation of asbestos and this was correlated with the dose of asbestos. With increasing time after instillation, however, release of IL-1 by pleural leucocytes gradually recovered to normal until, one month after asbestos injection, the leucocytes produced augmented IL-1 in culture compared with control pleural leucocytes. Our data show that pleural leucocytes possess the potential to produce IL-1 in vitro and this capability is altered by intratracheal instillation of crocidolite asbestos. This may be relevant to development of pleural diseases associated with inhalation of asbestos.
Department of Medicine (RIE), University of Edinburgh, City Hospital, UK.
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Donaldson
D000818:Animals; D001194:Asbestos / Q000494:pharmacology*; D017638:Asbestos, Crocidolite; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007375:Interleukin-1 / Q000096:biosynthesis*; D007962:Leukocytes / Q000378:metabolism*; D016131:Lymphocyte Subsets; D051381:Rats; D013997:Time Factors
D007375:Interleukin-1; D017638:Asbestos, Crocidolite; D001194:Asbestos