The effect of cyclic GMP and cyclic AMP on migration by electroporated human neutrophils.
Human neutrophils retain the ability to migrate when they are electroporated; this enables the study of potential mediators by direct application to the cell interior. Cyclic GMP strongly enhances random migration by electroporated human neutrophils. The effect is maximal at a concentration of 10 microM. The potentiating effect of cGMP is chemokinetic. Chemotaxis of electroporated neutrophils activated by formylmethionylleucylphenylalanine (fMLP) is stimulated by cGMP at concentrations up to 10 microM; higher concentrations inhibit chemotaxis. Cyclic AMP resembles cGMP in that both activation and inhibition may occur. However, activation occurs over a very small concentration range, and inhibition is a predominant feature. Cyclic nucleotide-activated migration is dependent on Ca2+, in contrast with activation of migration by fMLP.
Department of Medical Biochemistry, University of Leiden, The Netherlands.
J G
JG
Elferink
B M
BM
de Koster
D002118:Calcium / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects
D009240:N-Formylmethionine Leucyl-Phenylalanine; D000242:Cyclic AMP; D006152:Cyclic GMP; D002118:Calcium