Improvements in detection sensitivity for the determination of ivermectin in plasma using chromatographic techniques and laser-induced fluorescence detection with automated derivatization.
Improvement in detection sensitivity for the analysis of ivermectin was observed through utilization of laser-induced fluorescence detection and by manipulation of chromatographic conditions. Gradient elution used in combination with narrow-bore chromatography and conventional fluorescence detection resulted in a limit of quantitation for the major homologue of ivermectin of 0.01 ng/ml in dog plasma. Laser-induced fluorescence detection with isocratic chromatographic conditions also resulted in a limit of quantitation of 0.01 ng/ml in dog plasma, which is a six-fold improvement over previously reported methods. Introduction of an automated procedure for the derivatization and injection of samples reduced the amount of sample handling, eliminated the potential for analyte/internal standard degradation and contributed to the overall ease of analysis.
Rabel
S R
SR
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
Stobaugh
J F
JF
Heinig
R
R
Bostick
J M
JM
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D007559:Ivermectin / Q000097:blood*; D007834:Lasers; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D007559:Ivermectin