Determination of hydrazine in human plasma by high-performance liquid chromatography.
A simple and sensitive liquid chromatographic method for the determination of hydrazine in human plasma and serum is described. Samples were prepared in a single-step reaction by protein denaturation with trichloroacetic acid and derivatization to a stable azine with 4-hydroxybenzaldehyde. Chromatographic separation was carried out on a reversed-phase (octadecylsilane) column with methanol-water (60:40) as mobile phase and ultraviolet detection at 340 nm. Linearity was found in the range 5-1000 ng/ml. The detection limit of spiked plasma was 1 ng/ml. The coefficient of variation ranged from 1.7 to 3.8%. No degradation of hydrazine was found in spiked plasma and serum, even after storage at room temperature for one week. An increased hydrazine level was found after in vitro degradation from isoniazid in human serum.
Medizinisches Labor Dres. Schiwara, Bremen, Germany.
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Kirchherr
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D006834:Hydrazines / Q000097:blood*; D016273:Occupational Exposure; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D006834:Hydrazines; C029424:hydrazine