Rapid detection of human group C rotaviruses by reverse passive hemagglutination and latex agglutination tests using monoclonal antibodies.
Reverse passive hemagglutination (RPHA) tests and a latex agglutination test with monoclonal antibodies (MAbs) were developed for the rapid detection of noncultivatable human group C rotaviruses. For RPHA tests, two MAbs, MAb 5A12 recognizing the outer capsid and MAb 13A3 recognizing the inner capsid, were separately used for the coating of sheep erythrocytes (SRBCs). Forty-six fecal samples were examined to confirm the practicality of the tests. As a result, there was concordance between the RPHA test with SRBCs coated with MAb 5A12 and polyacrylamide gel electrophoresis of viral RNA (RNA-PAGE) in 44 (95.6%) of 46 samples, while the diagnoses by the RPHA test with SRBCs coated with MAb 13A3 were in complete agreement with those by RNA-PAGE. Furthermore, a latex agglutination test with MAb 13A3 was also developed, and this test was fast enough and sensitive enough to successfully detect the viruses from most fecal samples within 2 min. The present procedures would be useful for the diagnosis of human group C rotavirus infections in clinical laboratories which are not well equipped.
Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Japan.
M
M
Kuzuya
R
R
Fujii
M
M
Hamano
T
T
Nagabayashi
H
H
Tsunemitsu
M
M
Yamada
S
S
Nii
T
T
Mori
D000911:Antibodies, Monoclonal; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D005759:Gastroenteritis / Q000175:diagnosis; D006386:Hemagglutination Tests / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007223:Infant; D007841:Latex Fixation Tests / Q000379:methods* / Q000706:statistics & numerical data; D012401:Rotavirus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D000911:Antibodies, Monoclonal