An indirect sandwich ELISA for the identification of bovine enteroviruses.
An indirect sandwich ELISA is described for the detection of bovine enteroviruses. The assay was developed as an alternative to the complement fixation test and proved to be more sensitive and convenient. Ten bovine enterovirus prototype strains were easily discriminated. No cross-reactions were observed with other picornaviruses including foot-and-mouth disease viruses, swine vesicular disease virus, porcine enteroviruses and bovine rhinovirus.
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey UK.
M C
MC
Höfner
W C
WC
Carpenter
S A
SA
Lyons
C
C
Hamblin
D000818:Animals; D002417:Cattle; D004770:Enterovirus / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*