RNA insertions and gene duplications in the nonstructural protein p125 region of pestivirus strains and isolates in vitro and in vivo.
Sixteen cytopathogenic (cp) bovine viral diarrhea virus (BVDV) strains/isolates were screened for the existence of RNA insertions in the p125 gene region and/or for p80 gene duplications using the polymerase chain reaction after reverse transcription. Three strains/isolates were shown to contain insertions, and in three others gene duplications were demonstrated. One strain was shown to contain a gene duplication in addition to an insertion. Insertions or duplications were absent in the genomes of 10 non-cp BVD virus strains/isolates, in 4 hog cholera (HC) virus strains, and in 9 of the 16 cp BVD virus strains/isolates analyzed. These results indicate that RNA insertions and gene duplications, although significant, are not the only mechanism contributing to cytopathogenicity in vitro. The RT-PCR was also applied to RNA isolated from tissues of a calf suffering from mucosal disease. Insertion-containing amplification products could be demonstrated in an organ-specific pattern.
Institute of Virology, Hannover Veterinary School, Germany.
I
I
Greiser-Wilke
L
L
Haas
K
K
Dittmar
B
B
Liess
V
V
Moennig
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000235:genetics*; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics*; D005814:Genes, Viral; D007668:Kidney; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins