Immunohistochemical localization of calretinin-, calbindin-D28k- and parvalbumin-containing cells in the hypothalamic paraventricular and supraoptic nuclei of the rat.
The localization of three calcium-binding proteins, calretinin, calbindin-D28k and parvalbumin, in the hypothalamic paraventricular and supraoptic nuclei of the rat was immunohistochemically examined on adjacent sections and their distribution patterns were compared. Overlap between the distribution of calretinin-immunoreactive cells and that of calbindin-D28k-immunostained cells was found in the rostrodorsal part of the supraoptic nucleus, and the caudoventral part of this nucleus contained predominantly calbindin-D28k-stained cells. Cells of the medial and lateral magnocellular subdivisions of the paraventricular nucleus were almost devoid of the calcium-binding proteins examined. No parvalbumin-immunostained cells were observed in either nucleus. This study provides a further characterization of cell bodies in the hypothalamic paraventricular and supraoptic nuclei.
Department of Anatomy, Fujita Health University School of Medicine, Aichi, Japan.
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Arai
D M
DM
Jacobowitz
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S
Deura
D000818:Animals; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D005136:Eye Proteins / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000033:anatomy & histology / Q000378:metabolism*; D010320:Parvalbumins / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000276:immunology / Q000378:metabolism*; D013495:Supraoptic Nucleus / Q000033:anatomy & histology / Q000378:metabolism*
C576500:Calb1 protein, rat; C576512:Calb2 protein, rat; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D005136:Eye Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G