Localization of a retroviral element within the rd gene coding for the beta subunit of cGMP phosphodiesterase.
Retinal degeneration in the rd mouse is inherited as an autosomal recessive trait and is caused by a defect in the gene encoding the beta subunit of cGMP phosphodiesterase. Recently, a close genetic association of the rd gene with an endogenous xenotropic murine leukemia virus (Xmv-28) was established by linkage analysis using recombinant inbred strains of mice. In this study, genomic DNA mapping and sequence analyses clarify the position of the proviral sequences in relation to the rd gene. We find that the Xmv-28 provirus is integrated into intron I of the rd gene 1511 bp downstream of the exon-intron boundary. The transcriptional orientation of the provirus is opposite to that of the gene for the beta subunit of cGMP phosphodiesterase. Reverse transcription-PCR demonstrates that the integrated Xmv-28 sequences are transcribed in the retina. The provirus is present in every strain of rd mouse tested.
Jules Stein Eye Institute, University of California School of Medicine, Los Angeles 90024.
C
C
Bowes
T
T
Li
W N
WN
Frankel
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Danciger
J M
JM
Coffin
M L
ML
Applebury
D B
DB
Farber
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003433:Crosses, Genetic; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D004247:DNA / Q000235:genetics; D005260:Female; D005796:Genes; D007438:Introns; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010727:Phosphoric Diester Hydrolases; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics* / Q000302:isolation & purification; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D004247:DNA; D010727:Phosphoric Diester Hydrolases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; C517438:Pde6b protein, mouse