Increase of calcium levels at interphase in NIH 3T3 cells.
The fluorescent calcium ion indicator dye Fluo-3 and DNA-binding dye Hoechst 33342 were employed to determine, in a quantitative microspectrofluorometric study, the intracellular calcium ion concentration ([Ca2+]i) and the DNA content of individual living NIH3T3 cells. The well-separated excitation and emission properties of these dyes allowed us to establish for each cell both the phase of the cell cycle using DNA content and [Ca2+]i. We found that the transition from G1, through S, to the G2 phase is accompanied by a two-fold increase in [Ca2+]i. The [Ca2+]i was inhomologous in each phase of the interphase (G1, S and G2) although [Ca2+]i in the S and G2 phases was never lower than certain threshold values in the G1 and S phases respectively. [Ca2+]i in G0 cells was lower than that in G1 cells. These changes in [Ca2+]i suggest that [Ca2+]i may be an important regulator of cell cycle progression.
Xue
S B
SB
Department of Biology, Beijing Normal University, PRC.
Zhang
H Q
HQ
Cheng
R X
RX
Li
S W
SW
D016475:3T3 Cells / Q000166:cytology / Q000378:metabolism*; D000814:Aniline Compounds; D000818:Animals; D001562:Benzimidazoles; D002118:Calcium / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005456:Fluorescent Dyes; D007399:Interphase; D051379:Mice; D013050:Spectrometry, Fluorescence / Q000379:methods; D014966:Xanthenes
D000814:Aniline Compounds; D001562:Benzimidazoles; D005456:Fluorescent Dyes; D014966:Xanthenes; C059715:Fluo-3; D004247:DNA; C017807:bisbenzimide ethoxide trihydrochloride; D002118:Calcium