Phenotypic and functional consequences of herpesvirus saimiri infection of human CD8+ cytotoxic T lymphocytes.
Herpesvirus saimiri (HVS) was used to infect and transform human CD8+ cytotoxic T lymphocytes (CTL), and the phenotypic and functional consequences of HVS infection of CD8+ T lymphocytes were investigated. HVS-transformed CTL no longer require antigen restimulation yet maintain their phenotype and HLA-restricted cytolytic function and specificity. The ability of HVS to transform CTL may have an important role in the functional analysis of human antigen-specific CTL.
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
K R
KR
Berend
J U
JU
Jung
T J
TJ
Boyle
J M
JM
DiMaio
S A
SA
Mungal
R C
RC
Desrosiers
H K
HK
Lyerly
D015703:Antigens, CD / Q000032:analysis*; D016827:CD8 Antigens / Q000032:analysis*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D004279:DNA, Viral / Q000032:analysis; D005434:Flow Cytometry; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*
D015703:Antigens, CD; D016827:CD8 Antigens; D004279:DNA, Viral; D007376:Interleukin-2; D011994:Recombinant Proteins