Enzyme rate assay for 5-fluorocytosine.
Modification of an enzyme assay for 5-fluorocytosine, from an end point to a rate analysis, has resulted in a method that can provide results within 10 minutes. The modification also rendered the assay less susceptible to interference from bilirubin and triglycerides. This obviated the need to correct 5-fluorocytosine results for icteric or moderately lipemic samples. The performance history of the enzyme rate method has shown that it is reliable, quick, cost-effective, and suitable for use in a clinical laboratory.
Department of Laboratory Medicine, University Hospital, Vancouver, British Columbia, Canada.
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Shivji
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Bernstein
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Noble
D000619:Aminohydrolases / Q000097:blood*; D001681:Biological Assay / Q000379:methods*; D005437:Flucytosine / Q000008:administration & dosage / Q000097:blood*; D006801:Humans
D005437:Flucytosine; D000619:Aminohydrolases; C023157:creatinine deiminase