Crystallization and preliminary X-ray analysis of a microbial superantigen staphylococcal enterotoxin C2.
High yields of staphylococcal enterotoxin C2, from Staphylococcus aureus, have been purified using dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the tetragonal space group P4(1)22 with unit cell dimensions a = b = 43.2 A and c = 290.9 A with one molecule per asymmetric unit. The crystals diffract to at least 2.8 A resolution and are suitable for three-dimensional X-ray structural analysis.
Department of Biochemistry, University of Bath, Claverton Down, Avon, U.K.
E F
EF
Passalacqua
R D
RD
Brehm
K R
KR
Acharya
H S
HS
Tranter
D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D004768:Enterotoxins / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction
D000942:Antigens, Bacterial; D004768:Enterotoxins; C034959:enterotoxin C, staphylococcal