Similarity of the 5' and 3'-TAR secondary structures in HIV-1.
The structure of the TAR RNA element transcribed at the 5' end of the R region of the human immunodeficiency virus is compared to the structure of its duplicate sequence in the 3' R region of the viral genome. Based on the 5'-TAR secondary structure already described, we assessed by RNase T1 primer extension assay the degree of similarity between the 5'-TAR and the 3'-TAR RNA secondary structures. We also analysed the influence of modifications in the flanking sequences. We show that the secondary structures of the 5'-TAR and the 3'-TAR are very similar and are not influenced by the flanking sequences.
ICGM, INSERM U 363, Université PARIS V, Hopital COCHIN, Paris, France.
P
P
Wang
M C
MC
Rouyez
S
S
Ducamp
S
S
Saragosti
M
M
Ventura
D001482:Base Composition; D001483:Base Sequence; D016679:Genome, Viral; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry*; D014158:Transcription, Genetic; D000099253:Trans-Activation Responsive RNA-Binding Protein
D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D000099253:Trans-Activation Responsive RNA-Binding Protein