The glycoprotein B homologue of human herpesvirus 6.
The gene for the homologue of herpesvirus glycoprotein B (gB) has been identified in the genome of human herpesvirus 6 (HHV-6), strain U1102, and the nucleotide sequence was determined. The open reading frame encodes a protein of 830 amino acids (93.2K) with the characteristics of a transmembrane glycoprotein and close similarity to the gp58/116 complex of human cytomegalovirus (HCMV). Monoclonal antibodies 2D10 and 2B9 have been shown previously to react with an HHV-6 glycoprotein of apparent M(r) 112K, and its proteolytic cleavage products of M(r) 64K and 58K. We show that both monoclonal antibodies detect prokaryotically expressed carboxy-terminal fragments of the HHV-6 gB homologue. This indicates that the HHV-6 gB homologue is probably processed by proteolytic cleavage similar to its equivalents in HCMV and various other herpesviruses.
Institut für klinische und molekulare Virologie, Universität Erlangen-Nürnberg, Germany.
K
K
Ellinger
F
F
Neipel
L
L
Foà-Tomasi
G
G
Campadelli-Fiume
B
B
Fleckenstein
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002874:Chromosome Mapping; D015654:Herpesvirus 6, Human / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011499:Protein Processing, Post-Translational / Q000502:physiology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins; C077311:glycoprotein B, human herpesvirus-6