Stereoselective distribution of acenocoumarol enantiomers in human plasma: chiral chromatographic analysis of the ultrafiltrates.
Stereoselectivities for the binding of rac-acenocoumarol to human serum albumin (HSA), alpha 1-acid glycoprotein (AGP), and human plasma were determined by chiral HPLC analysis of the ultrafiltrates on a Chiral-AGP column. The results confirmed the previously detected inverse stereoselectivities; for HSA the ratio of the enantiomeric constants was KR/KS approximately 2, while for AGP it was KR/KS approximately 0.3. In plasma the contribution of HSA dominates, although in pathological states, elevated AGP levels may compensate for stereoselective distribution.
Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.
I
I
Fitos
J
J
Visy
M
M
Simonyi
J
J
Hermansson
D000074:Acenocoumarol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008024:Ligands; D009961:Orosomucoid / Q000737:chemistry; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014462:Ultrafiltration
D008024:Ligands; D009961:Orosomucoid; D012709:Serum Albumin; D000074:Acenocoumarol