Cytokine production during intraperitoneal chemohyperthermia.
The aim of the present work is to investigate in vivo cytokine production during chemohyperthermia. 11 patients suffering from gastric adenocarcinoma (n = 6), ovarian adenocarcinoma (n = 4) or malignant mesothelioma (n = 1) were studied. Patients received 60 mg mitomycin or 100 mg cisplatin per square meter during 2 h in 6 liters of a heating solution (temperature 42 degrees C, flow rate 200 ml/min in a closed circuit) after previous surgical resection. Tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were measured at 0, 30, 45, 60 and 90 min, both in the blood stream and in the heating solution circulating intraperitoneally. We observed a slight increase in plasma IL-6 occurring as soon as 30 min, a dramatic rise in IL-6 in the heating solution. TNF-alpha values were only slightly augmented. In addition, the importance of various factors in the induction of IL-6 and TNF-alpha production during chemohyperthermia (temperature, mitomycin C, cisplatin) were studied using a whole blood ex vivo model.
Coulon
L
L
Department of Immunology, Centre Hospitalier Lyon-Sud, Pierre-Bénite, France.
Bienvenu
J
J
Gutowski
M C
MC
Barbier
Y
Y
Barbier
M
M
Panteix
G
G
Gilly
F N
FN
Carry
P Y
PY
Sayag
A
A
Braillon
G
G
D000230:Adenocarcinoma / Q000378:metabolism* / Q000628:therapy; D002945:Cisplatin / Q000008:administration & dosage; D003131:Combined Modality Therapy; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D007263:Infusions, Parenteral; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D008654:Mesothelioma / Q000378:metabolism* / Q000628:therapy; D008937:Mitomycins / Q000008:administration & dosage; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000628:therapy; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000628:therapy; D013696:Temperature; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D015850:Interleukin-6; D008937:Mitomycins; D014409:Tumor Necrosis Factor-alpha; D002945:Cisplatin