Methylprednisolone treatment of experimental spinal cord injury.
Lipid peroxidation has been identified as a deleterious consequence of contusive spinal cord trauma and of thermal injury. The L3-L6 spinal cord segment was thermally injured using a radiofrequency heating chamber mounted on the vertebral column of anesthetized rats. Hind limb function was assessed 2 hours later. A bolus of methylprednisolone (MP, 30 mg/kg) was then given intravenously, followed by 5.4 mg/kg/hr MP for 6 or 24 hours. Cord water content and regional spinal cord blood flow (RSCBF, 14C-butanol distribution) were measured at seven cord levels after function had been reassessed following treatment. Untreated rats were given vehicle. The study was randomized and blinded.


RESULTS
Edema in heated segments was progressive over 24 hours, but was the same in treated vs untreated rats. RSCBF in heated segments was the same in treated vs uninjured controls at 6 and 24 hours. In untreated rats, RSCBF in the heated segment was elevated by 30% at 6 hours, but was the same as uninjured control by 24 hours. In the unheated segments of untreated rats, RSCBF was elevated at 24 hours. At 24 hours, RSCBF was lower in treated vs untreated rats at all levels, including the heated one. Limb function deteriorated equivalently in both groups.


CONCLUSIONS
MP obviated the early rise in RSCBF in heated segments and the elevations in RSCBF in uninjured segments, but had no effect on cord edema or on limb function.
Department of Surgery, Washington University School of Medicine, St. Louis, Missouri.
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Iwai
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Monafo
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Eliasson
D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D001834:Body Water / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D004487:Edema / Q000517:prevention & control; D006614:Hindlimb / Q000502:physiology; D006358:Hot Temperature; D008297:Male; D008775:Methylprednisolone / Q000627:therapeutic use*; D011846:Radio Waves; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects; D012890:Sleep / Q000187:drug effects; D013116:Spinal Cord / Q000098:blood supply / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000503:physiopathology
D008775:Methylprednisolone