Expression of native dimers of bovine seminal ribonuclease in a eukaryotic cell system.
Bovine seminal ribonuclease, a uniquely dimeric pancreatic-like RNase, with its dimeric structure stabilized by two intersubunit disulfides, and endowed with special, i.e. non-catalytic, actions (antitumor, immunosuppressive, antispermatogenic), was stably expressed in Chinese hamster ovary cells. The recombinant protein, secreted in the culture medium as a correctly folded dimeric enzyme, was purified to homogeneity and found to be fully active both in its catalytic and antitumor activities.
Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Italy.
N
N
Russo
M
M
De Nigris
A
A
Di Donato
G
G
D'Alessio
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012260:Ribonucleases / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D012669:Seminal Vesicles / Q000201:enzymology*; D013539:Simian virus 40; D014162:Transfection
D046911:Macromolecular Substances; D011994:Recombinant Proteins; D012260:Ribonucleases