Comparison of the E-test and reference agar dilution method for susceptibility of gram-negative anaerobic organisms.
Minimal inhibitory concentrations (MICs) of clindamycin, cefoxitin, imipenem, and metronidazole were determined by the E-Test and a reference agar dilution method for 92 gram-negative anaerobic organisms. For 335 MIC pairs, the agreement between the two systems was 80.1%; 60 (17.9%) differed by two or more twofold dilutions. The best agreement was observed with imipenem (91.7%) and the poorest with cefoxitin (67%). With the E-Test (PDM Epsilometer for antimicrobial susceptibility testing [AB Bodisk, Solna, Sweden]), MICs for individual organisms are simple to achieve, although for certain antibiotics discrepancies with the reference method are unacceptable.
Department of Microbiology, Victoria Hospital, London, Ontario, Canada.
P R
PR
Couroux
V E
VE
Massey
B C
BC
Schieven
R
R
Lannigan
Z
Z
Hussain
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D002440:Cefoxitin / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D006089:Gram-Negative Anaerobic Bacteria / Q000187:drug effects*; D015378:Imipenem / Q000494:pharmacology; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D008795:Metronidazole; D002981:Clindamycin; D002440:Cefoxitin; D015378:Imipenem