Genetic stability and xanthan gum production in Xanthomonas campestris pv. campestris NRRL B1459.
A transposon (Tn5-SC) was constructed that can be used to quantify genetic deletions or amplifications. This transposon was used to evaluate the genomic stability of Xanthomonas campestris pv. campestris NRRL B1459 and we found that the genome of this bacterium is as stable as other Gram-negative bacteria or even more stable. Homologous recombination between plasmid sequences was determined in strain NRRL B1459 and was found to occur at a similar level to that reported for other Gram-negative bacteria. We report here that in X.c.c. NRRL B1459 there is no straightforward correlation between the occurrence of genetic rearrangements and frequency of homologous recombination. These data are discussed with respect to the reported instability of strain NRRL B1459 for xanthan gum production.
Departamento de Bioingeniería, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
J M
JM
Martínez-Salazar
A N
AN
Palacios
R
R
Sánchez
A D
AD
Caro
G
G
Soberón-Chavez
D002633:Chemotaxis / Q000235:genetics; D004249:DNA Damage; D004251:DNA Transposable Elements / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005784:Gene Amplification; D017353:Gene Deletion; D016680:Genome, Bacterial; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011995:Recombination, Genetic; D016959:Xanthomonas campestris / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D004251:DNA Transposable Elements; D009153:Mutagens; D011135:Polysaccharides, Bacterial; C002563:xanthan gum