Verification of the position of the phosphate group in some synthetic phosphopeptides by fast-atom bombardment and tandem mass spectrometry.
Some synthetically obtained linear and cyclic phosphopeptides of low molecular weight have been studied by fast-atom bombardment and tandem mass spectrometry to verify the position of the phosphate group in these compounds. Based upon the occurrence/non-occurrence of loss of phosphoric acid from low abundance fragment ions induced by low- and high-energy collisions with target gases, it is shown that the position of the phosphate group in the phosphopeptides studied can be determined unequivocally.
Nijenhuis
A A
AA
Institute of Mass Spectrometry, University of Amsterdam, The Netherlands.
Fokkens
R H
RH
Nibbering
N M
NM
van Oijen
A H
AH
de Bont
H B
HB
Liskamp
R M
RM
van Boom
J H
JH
D000595:Amino Acid Sequence; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010710:Phosphates / Q000032:analysis*; D010748:Phosphopeptides / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D010710:Phosphates; D010748:Phosphopeptides