Spin trapping of superoxide released by opsonized asbestos from human promyelocytic leukemia cell line, HL60.
By ESR using 5,5-dimethyl-1-pyrroline-1-oxide as a spin trap, superoxide (O2-) production was proved upon stimulation of dimethyl sulfoxide-differentiated HL60 by crocidolite opsonized with fresh or refrigerated serum, as well as by phorbol myristate acetate (PMA). Crocidolite, unopsonized or opsonized with frozen-thawed or heat-inactivated serum, did not induce O2- release. Addition of iron chelators or superoxide dismutase inhibited O2- release completely. Neither undifferentiated nor PMA-differentiated HL60 released O2- upon stimulation with opsonized crocidolite.
Department of Hygiene and Preventive Medicine, Osaka University School of Medicine, Japan.
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T
Takeuchi
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Morimoto
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Kosaka
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Shiga
D001194:Asbestos / Q000494:pharmacology*; D017638:Asbestos, Crocidolite; D002454:Cell Differentiation; D003497:Cyclic N-Oxides; D003676:Deferoxamine / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D004369:Pentetic Acid / Q000494:pharmacology; D013113:Spin Labels; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D003497:Cyclic N-Oxides; D013113:Spin Labels; D013481:Superoxides; D017638:Asbestos, Crocidolite; D001194:Asbestos; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D004369:Pentetic Acid; D003676:Deferoxamine; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide