Spin-trapping and chemiluminescence studies of neutrophils from a Holstein-Friesian calf with bovine leukocyte adhesion deficiency.
The ability of neutrophils from a Holstein-Friesian calf with bovine leukocyte adhesion deficiency (the proband with a genetic deficiency of the Mac-1 (CD11b/CD18) glycoprotein corresponding to the receptor of complement iC3b) to generate oxygen radicals was examined using electron spin resonance spectrometry (ESR) combined with a spin-trapping technique and luminol-dependent chemiluminescence spectrometry. When the neutrophils were stimulated with phorbol 12-myristate 13-acetate (PMA), an ESR spectrum confirming the generation of superoxide anions (O2-) was clearly observed in both healthy and diseased calves. However, when the neutrophils were stimulated by opsonized zymosan, appearance of the ESR spectrum was recognized in the healthy calves but not in the diseased calf. Similar results were obtained from chemiluminescence experiments.
Kuwabara
M
M
Department of Radiation Biology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Nishimura
Y
Y
Sato
F
F
Takahashi
T
T
Tajima
M
M
Takahashi
K
K
Nagahata
H
H
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D002448:Cell Adhesion; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D007964:Leukocytosis / Q000097:blood / Q000209:etiology / Q000662:veterinary*; D008163:Luminescent Measurements; D016177:Macrophage-1 Antigen / Q000097:blood*; D009504:Neutrophils / Q000502:physiology*; D009895:Opsonin Proteins; D017382:Reactive Oxygen Species / Q000378:metabolism; D013113:Spin Labels; D013481:Superoxides / Q000097:blood; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D003497:Cyclic N-Oxides; D016177:Macrophage-1 Antigen; D009895:Opsonin Proteins; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013481:Superoxides; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate