Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.
14-3-3 proteins are apparently ubiquitous eukaryotic proteins that comprise a large number of isoforms. They have been implicated in the regulation of a wide range of biological processes [reviewed in Aitken et al. (1992) Trends Biochem. Sci. 17, 498-501]. We have raised specific antibodies against each mammalian brain isoform of 14-3-3 employing peptides synthesised from the amino-terminal regions. The peptides were, like the proteins from mammalian brain, N-acetylated. The antiserum specific for the epsilon isoform did not recognise the recombinant form of this protein (lacking the N-acetyl co-translational modification) expressed in E. coli until it was chemically acetylated.
Martin
H
H
Laboratory of Protein Structure, National Institute for Medical Research, Mill Hill, London, UK.
Patel
Y
Y
Jones
D
D
Howell
S
S
Robinson
K
K
Aitken
A
A
D048948:14-3-3 Proteins; D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000276:immunology*; D010449:Peptide Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D014446:Tyrosine 3-Monooxygenase