A comparison of ELISA, FAST-ELISA and gel diffusion tests for detecting antibody to equine infectious anaemia virus.
Sera of sixteen horses with clinical signs of EIA from six different outbreaks and sera of 100 uninfected horses were used to validate an ELISA for EIA diagnosis. The antigen used was a recombinant protein derived from the amino-terminal portion of the transmembrane envelope protein of EIA (gp45). Reactivity between positive and negative sera could be clearly distinguished. Comparison with the traditional agar gel immunodiffusion test (commonly called the Coggins test) showed that the ELISA was superior in sensitivity. Comparison of this ELISA with the FAST-ELISA system showed that the latter was less sensitive. Although the FAST-ELISA was much faster to perform, it could not be recommended as a diagnostic test in its present form, because the margin between reactivity by a positive serum and a negative serum was not high.
Walter & Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Parkville, Vic., Australia.
A M
AM
Lew
L M
LM
Thomas
P J
PJ
Huntington
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D004196:Disease Outbreaks / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D004859:Equine Infectious Anemia / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D006736:Horses; D005779:Immunodiffusion / Q000662:veterinary*; D004860:Infectious Anemia Virus, Equine / Q000302:isolation & purification*
D000914:Antibodies, Viral