[The effect of inhibitors of DNA topoisomerase II on the radiosensitivity, repair and conformational changes in the chromatin in mammalian cells].
A comparative study was made of modification of the effects of UV-, X- and fission neutron-radiation by DNA-topoisomerase II and spermidine. The efficiency of the studied agents was the highest with UV-radiation of cells. Being insignificantly efficient in changing the radiosensitivity of cells, novobiocine and spermidine suppressed significantly the recovery of cells from radiation damages which were realized as chromosome aberrations upon X-ray dose fractionation. Adriamycin removed completely the effect of dose fractionation. Chromatin condensation was noted in cells after the treatment thereof with the agents under study. The authors discuss the possible mechanism of interaction between DNA-topoisomerase II and repair processes in mammalian cells.
Gil'iano
N Ia
NIa
Ikhtiar
A M
AM
Malinovskiĭ
O V
OV
D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D002843:Chromatin / Q000187:drug effects* / Q000528:radiation effects; D002869:Chromosome Aberrations; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004317:Doxorubicin / Q000494:pharmacology; D005214:Fast Neutrons; D006367:HeLa Cells / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D009675:Novobiocin / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects / Q000528:radiation effects; D011836:Radiation Tolerance / Q000187:drug effects*; D013095:Spermidine / Q000494:pharmacology; D059005:Topoisomerase II Inhibitors; D014466:Ultraviolet Rays
D002843:Chromatin; D059005:Topoisomerase II Inhibitors; D009675:Novobiocin; D004317:Doxorubicin; D013095:Spermidine