Detection of cutaneous and genital HPV types in clinical samples by PCR using consensus primers.
Two sets of consensus PCR primers consisting of a common 3' primer CP-I and two 5'-primers, CP-IIG (primer set A) and CP-IIS (primer set B), in the E1 open reading frame of the human papillomavirus (HPV) genome are presented. These two primer sets enabled the detection of a 188 base pair (bp) fragment of HPV 1, 2, 3, 4, 5, 6b, 7, 8, 9, 10a, 11, 12, 14a, 16, 17, 18, 19, 20, 21, 22, 24, 25, 31, 33, 36, 37, 38, 39 and 46. HPV types 15, 23, 49 and 50 were poorly amplified and HPV type 41 was not amplified. The method is suitable for the detection of HPV DNA sequences in clinical samples of both cervical and cutaneous lesions.
Department of Dermatology, University Hospital Leiden, The Netherlands.
L M
LM
Tieben
J
J
ter Schegget
R P
RP
Minnaar
J N
JN
Bouwes Bavinck
R J
RJ
Berkhout
B J
BJ
Vermeer
M F
MF
Jebbink
H L
HL
Smits
D001483:Base Sequence; D002584:Cervix Uteri / Q000382:microbiology*; D016384:Consensus Sequence; D015342:DNA Probes; D004279:DNA, Viral / Q000145:classification / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid; D014860:Warts / Q000382:microbiology*
D015342:DNA Probes; D004279:DNA, Viral