Expression cloning of an epithelial amiloride-sensitive Na+ channel. A new channel type with homologies to Caenorhabditis elegans degenerins.
A complementary DNA encoding an amiloride-sensitive Na+ channel has been cloned and characterized from rat colon. The protein encoded by the cDNA has a sequence of 699 amino acids (79 kDa) containing several putative membrane spanning domains and potential phosphorylation sites. It forms a channel that has the electrophysiological and pharmacological properties characteristic of the epithelial Na+ channel. Homologies (including in transmembrane domains) have been found between a part of the channel sequence and the Mec4 gene product of Caenorhabditis elegans, a protein associated with mutation-induced neuronal degeneration.
Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
E
E
Lingueglia
N
N
Voilley
R
R
Waldmann
M
M
Lazdunski
P
P
Barbry
D000584:Amiloride / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000737:chemistry* / Q000235:genetics; D029742:Caenorhabditis elegans Proteins; D003001:Cloning, Molecular; D003106:Colon / Q000737:chemistry / Q000187:drug effects; D004247:DNA; D053503:Epithelial Sodium Channels; D004848:Epithelium / Q000737:chemistry / Q000187:drug effects; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D015222:Sodium Channels / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D014981:Xenopus
D029742:Caenorhabditis elegans Proteins; D053503:Epithelial Sodium Channels; D015801:Helminth Proteins; C115871:Mec-4 protein, C elegans; D008565:Membrane Proteins; C440894:Scnn1a protein, rat; D015222:Sodium Channels; D000584:Amiloride; D004247:DNA