Giardiavirus double-stranded RNA genome encodes a capsid polypeptide and a gag-pol-like fusion protein by a translation frameshift.
Giardiavirus is a small, nonenveloped virus comprising a monopartite double-stranded RNA genome, a major protein of 100 kDa, and a less abundant polypeptide of 190 kDa. It can be isolated from the culture supernatant of Giardia lamblia, a parasitic flagellate in human and other mammals, and efficiently infects other virus-free G. lamblia. A single-stranded copy of the viral RNA can be electroporated into uninfected G. lamblia cells to complete the viral replication cycle. Giardiavirus genomic cDNA of 6100 nt was constructed and its sequence revealed the presence of two large open reading frames that are separated by a -1 frameshift and share an overlap of 220 nt. The 3' open reading frame contains all consensus RNA-dependent RNA polymerase sequence motifs. A heptamer-pseudoknot structure similar to those found at ribosomal slippage sites in retroviruses and yeast killer virus was identified within this overlap. Immunostudies using antisera against synthesized peptides from four regions in the two open reading frames indicated that the 100- and 190-kDa viral proteins share a common domain in the amino-terminal region. But the 190-kDa protein makes a -1 switch of its reading frame beyond the presumed slippage heptamer and is therefore a -1 frameshift fusion protein similar to the gag-pol fusion protein found in retroviruses.
Wang
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Department of Pharmaceutical Chemistry, University of California, San Francisco 94143-0446.
Yang
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Shen
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Wang
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D017124:Conserved Sequence; D016368:Frameshift Mutation; D015685:Fusion Proteins, gag-pol / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D016679:Genome, Viral; D016829:Giardia lamblia / Q000382:microbiology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012328:RNA Viruses / Q000235:genetics* / Q000472:pathogenicity; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D015685:Fusion Proteins, gag-pol; D012330:RNA, Double-Stranded; D012367:RNA, Viral