Synthesis and assembly of infectious bovine papillomavirus particles in vitro.
Bovine papillomavirus type 1 (BPV-1) virions were produced in vitro using vaccinia virus (VV) recombinants expressing the BPV-1 L1 and L2 capsid proteins. Particles morphologically resembling papillomaviruses were observed in the nucleus of cells infected with a VV recombinant for the BPV-1 L1 protein, and greater numbers of similar particles were seen in the nuclei of cells infected with a VV double recombinant for L1 and L2. Virus-like particles (VLPs) assembled in cells infected with the VV double recombinant for BPV-1 L1 and L2, and not those assembled in cells infected with the VV recombinant for BPV-1 L1 alone, were able to package BPV-1 DNA. Transcription of the BPV-1 E1 viral open reading frame was observed after a mouse fibroblast cell line was exposed to VLPs produced using a BPV-1 L1/L2 VV recombinant in a cell line containing episomal BPV-1 DNA. E1 transcription was not observed when the VLPs were pre-incubated with antibodies to the capsid protein of BPV-1. This system should allow an in vitro approach to the definition of the BPV-1 cellular receptor.
Zhou
J
J
Papillomavirus Research Unit, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
Stenzel
D J
DJ
Sun
X Y
XY
Frazer
I H
IH
D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000254:growth & development / Q000648:ultrastructure*; D002213:Capsid / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011994:Recombinant Proteins / Q000378:metabolism; D014616:Vaccinia virus; D014779:Virus Replication
D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins