Expression of human minor histocompatibility antigen on cultured kidney cells.
Incompatibility of human minor histocompatibility (hmH) antigens can induce rejection of grafts in organ transplantation and graft-versus-host reactions in bone marrow transplantation. In spite of their importance in clinical transplantation, hmH antigens are not well studied. Previous studies have demonstrated the expression of hmH antigens on T and B cells, hematopoietic progenitor cells and keratinocytes. We have for the first time demonstrated the expression of hmH antigens on cultured kidney cells using HLA-B35-restricted, hmH antigen-specific cytotoxic T lymphocyte (CTL) clones, which were previously established from a patient who rejected two kidneys from HLA-identical sisters. The CTL clones could not kill cultured kidney cells. Since cultured kidney cells expressed very low levels of HLA class I antigens it was thought that their failure to be killed by the CTL clones was due to lack of expression of HLA-B35 antigens. After induction of class I antigens on cultured kidney cells by interferon-gamma (IFN-gamma), the IFN-gamma-treated cultured kidney cells were killed by the CTL clones. Furthermore, we isolated hmH antigens as peptides from cultured kidney cells after treatment with IFN-gamma. These results indicate that cultured kidney cells express hmH antigens when HLA class I antigen is induced by IFN-gamma and hmH antigens on cultured kidney cells are recognized by T cells as peptides presented by HLA-B35 molecules.
Department of Tumor Biology, University of Tokyo, Japan.
Y
Y
Beck
M
M
Sekimata
S
S
Nakayama
G A
GA
Müller
C A
CA
Müller
J
J
Yamamoto
T
T
Nagao
H
H
Uchida
N
N
Akiyama
A
A
Kariyone
D000911:Antibodies, Monoclonal; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D006084:Graft Rejection / Q000276:immunology; D015797:HLA-B35 Antigen / Q000276:immunology; D004854:Herpesvirus 4, Human; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D007668:Kidney / Q000276:immunology*; D015778:Minor Histocompatibility Antigens / Q000276:immunology* / Q000302:isolation & purification; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D015797:HLA-B35 Antigen; D015778:Minor Histocompatibility Antigens; D007371:Interferon-gamma