Chronic ethanol consumption decreases the phorbol ester binding to membranal but not cytosolic protein kinase C in rat brain.
We examined the effect of 60 days of ethanol treatment on protein kinase C (PKC) in membrane and cytosolic fractions of the rat cerebral cortex. Membranal and cytosolic PKC were determined by binding technique using [3H]-phorbol 12,13 dibutyrate (PDBU) as radioligand and phorbol 12-myristate 13-acetate (PMA) as displacer. Chronic ethanol consumption resulted in a decrease in the maximum number of binding sites (Bmax) of [3H]-PDBU binding to membranal PKC without significant change in the apparent dissociation constant (KD) in the rat cortex. We also observed that chronic ethanol consumption had no significant effect on Bmax or KD of [3H]-PDBU binding to cytosolic PKC in the rat cerebral cortex. These results suggest that chronic ethanol consumption leads to the down-regulation of brain PKC associated with membrane but not with cytosol.
Pandey
S C
SC
Illinois State Psychiatric Institute, University of Illinois, Chicago 60651.
Dwivedi
Y
Y
Piano
M R
MR
Schwertz
D W
DW
Davis
J M
JM
Pandey
G N
GN
D000437:Alcoholism / Q000201:enzymology*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology*; D000431:Ethanol / Q000008:administration & dosage; D008297:Male; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism; D010703:Phorbol Esters / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism
D010703:Phorbol Esters; D015240:Phorbol 12,13-Dibutyrate; D000431:Ethanol; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate