Serotype analysis of rotaviruses from different locations in Malaysia.
The distribution of rotavirus G (VP7) serotypes circulating in four locations in Malaysia, representing three geographical areas, was evaluated in 341 RNA-positive stool specimens obtained discontinuously between 1977 and 1988 from infants and young children under the age of five years who were hospitalized with acute gastroenteritis. A total of 306 specimens (256 stool suspensions and 50 that were adapted to growth in tissue culture) that were rotavirus positive by the confirmatory enzyme-linked immunosorbent assay (ELISA) were examined for serotype by ELISA utilizing monoclonal antibodies to rotavirus G serotype 1, 2, 3, 4, or 9. One hundred eighty (59%) of the 306 specimens could be serotyped; of these 180 specimens, 71% were serotype 4, 15% were serotype 1, 4% were serotype 2, and 4% were serotype 3. Serotype 9 rotavirus was not detected. Most (71%) of the specimens tested were obtained in 1988, when serotype 4 predominated in three locations in West Malaysia; no single serotype was predominant in a limited number of specimens from East Malaysia.
Department of Genetics and Cellular Biology, University of Malaya, Kuala Lumpur, Malaysia.
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Rasool
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Kapikian
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000302:isolation & purification; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008296:Malaysia / Q000453:epidemiology; D012401:Rotavirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral