Mapping of the functional domains of the alpha 4 protein of herpes simplex virus 1.
Truncated alpha 4 genes were introduced into BHK tk- cells along with the neomycin phosphotransferase gene, that confers resistance to the eukaryotic antibiotic G418, driven by the HSV-1 beta tk promoter (beta tk- neor). Stably transformed cell lines were obtained and studied for the ability of the resident truncated alpha 4 genes to regulate the expression of the beta tk- neor, and for the ability of the truncated alpha 4 polypeptides to localize to the nuclei of transformed cells. The results indicated that the domain(s) for beta gene induction and for nuclear localization of the alpha 4 protein are located within the N-terminal 288 amino acids of the protein.
Department of Biology, Aristotelian University, Thessaloniki, Greece.
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Sivropoulou
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Arsenakis
D000818:Animals; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D017874:Immediate-Early Proteins; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection; D014709:Vero Cells; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics* / Q000378:metabolism
D017874:Immediate-Early Proteins; D054334:Viral Regulatory and Accessory Proteins; C048925:herpes simplex virus, type 1 protein ICP4; D010770:Phosphotransferases; D019868:Kanamycin Kinase