Alteration of membrane transductive mechanisms induced by ethanol in human lymphocyte cultures.
Ethanol, in millimolar concentrations, significantly modifies different transductive systems in human lymphocyte cultures. In particular, the presence of alcohol in the medium more than doubles the [Ca2+]i (from 70-90 to 200-250 nM), increasing Ca2+ fluxes from outside, and inhibits the active transport carried out by the calcium pump. The Ca2+ release from intracellular stores is not involved because 10 mM EGTA in the medium completely abolished the rise of [Ca2+]i. Since IP3 levels and cAMP concentrations are also involved in ethanol events (although with opposite effects), it seems that the alcohol may have a specific target on cell membranes (G-proteins) which influence many transductive pathways.
Istituto di Biologia Cellulare, Università degli Studi di Perugia, Italy.
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Fanò
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Belia
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Mariggiò
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Antonica
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Agea
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Spinozzi
D000428:Alcohol Drinking / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D010716:Phosphatidylinositols / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects*
D010716:Phosphatidylinositols; D000431:Ethanol; D019204:GTP-Binding Proteins; D000252:Calcium-Transporting ATPases; D002118:Calcium