2-Amino-3-methylimidazo[4,5-f]quinoline (IQ), a carcinogenic pyrolysate, induces chromosomal aberrations in Chinese hamster lung fibroblasts in vitro.
The ability of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) to induce chromosomal aberrations (CAs) in Chinese hamster lung fibroblast (CHL/IU) cells in vitro was examined. On incubation with rat S9 (2.5-10%, v/v) for 3 h, followed by a recovery culture period of 21 h, IQ caused significant induction of CAs at a concentration 20 micrograms/ml, but had less effect at 40 micrograms/ml. With longer recovery culture times such as 27-33 h, however, IQ was much more effective at 40 micrograms/ml. No significant induction was observed with 1 or 6 h treatments followed by 23 or 18 h recovery cultures, respectively. On incubation without S9, only weak CA induction by IQ was observed. These results show that IQ is a clastogen and that its clastogenic effect varied with the experimental conditions, such as the time of exposure and the time of recovery culture. The cell cycle perturbation effect is suggested to be one of the critical factors for the detection of the clastogenic potential of IQ.
Miura
K F
KF
Chromosome Research Center, Olympus Optical Co. Ltd, Tokyo, Japan.
Hatanaka
M
M
Otsuka
C
C
Satoh
T
T
Takahashi
H
H
Wakabayashi
K
K
Nagao
M
M
Ishidate
M
M
D000818:Animals; D002273:Carcinogens; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D066298:In Vitro Techniques; D007700:Kinetics; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens; D011804:Quinolines / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats
D002273:Carcinogens; D009153:Mutagens; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline