Shadow bands seen when typing polymorphic dinucleotide repeats: some causes and cures.
Polymorphic dinucleotide repeats are generally typed by using the PCR to generate products that are resolved on denaturing acrylamide gels. The presence of "shadow bands" on these gels often makes it difficult to score alleles reliably. We have developed procedures that overcome many of these difficulties. Important aspects of these improved procedures include using gels containing formamide as well as urea, transferring the resolved allelic fragments to nylon membranes by capillary blotting and probing the membranes with locus-specific oligonucleotide probes.
Department of Biochemistry, Oregon Health Sciences University, Portland 97201-3098.
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Litt
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Hauge
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Sharma
D001483:Base Sequence; D004586:Electrophoresis; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D009841:Oligonucleotides