Trichuris suis: a zinc metalloprotease from culture fluids of adult parasites.
A zinc metalloendoprotease has been isolated from in vitro culture fluids of Trichuris suis adults. The protease was purified from total culture fluids by passage through a cation exchange high-pressure liquid chromatography column. The 45-kDa protease has a pH optimum of 7.0 and an isoelectric point of 8.0 and was localized to the stichosome of the parasites using immunohistochemistry techniques.
Hill
D E
DE
United States Department of Agriculture, Beltsville Agricultural Research Center-East, Maryland 20705.
Gamble
H R
HR
Rhoads
M L
ML
Fetterer
R H
RH
Urban
J F
JF
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D008297:Male; D008666:Metalloendopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D014258:Trichuris / Q000201:enzymology*
D008666:Metalloendopeptidases