[The myocardial protective effects of cardiac tissue ACE inhibition in experimental ischemia-reperfusion in isolated rat hearts].
The protective effects of captopril were evaluated in vitro on isolated perfused rat hearts after a global ischemia of 20 min. The hearts were randomly allocated in 2 groups. In the first one (n = 6) captopril was added at a concentration of 270 microM. The second one was utilized as control (n = 6). Aortic flow and minute work respectively decreased on reperfusion by 35% and 49% in captopril group and by 65% and 71% in controls (p < 0.001). No changes occurred in heart rate. Aortic systolic pressure and coronary flow decreased in the 2 groups, but not significantly. Myocardial enzyme release during reperfusion showed significant lower levels of CPK and LDH in the captopril group as compared to controls (p < 0.001 after 41 min). The occurrence of serious ventricular arrhythmias was considerably higher in controls with respect to the captopril group. Irreversible ventricular fibrillation occurred only in control hearts (50%). These data indicate that captopril exerts a protective effect during myocardial ischemia and reperfusion by preventing serious ventricular arrhythmias, reducing enzymatic release and a lower decrease in cardiac performance, without an increase in heart rate.
Istituto di Clinica Cardiovascolare, Università degli Studi G. d'Annunzio, Chieti.
P
P
Di Napoli
G
G
Di Gregorio
F
F
De Sanctis
S
S
Gallina
E
E
Di Girolamo
G P
GP
Trevi
A
A
Barsotti
D000818:Animals; D002216:Captopril / Q000627:therapeutic use*; D003402:Creatine Kinase / Q000187:drug effects / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D006321:Heart / Q000187:drug effects*; D006439:Hemodynamics / Q000187:drug effects; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D009206:Myocardium / Q000201:enzymology*; D007703:Peptidyl-Dipeptidase A / Q000187:drug effects / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats
D002216:Captopril; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D007703:Peptidyl-Dipeptidase A