A receptor tyrosine kinase found in breast carcinoma cells has an extracellular discoidin I-like domain.
We have identified a breast carcinoma tyrosine phosphoprotein, discoidin domain receptor (DDR), that defines an unusual class of receptor tyrosine kinases. The DDR cDNA predicts a C-terminal tyrosine kinase domain and an N-terminal domain similar to the Dictyostelium discoideum lectin discoidin I. These domains are connected by an extraordinary hydrophilic proline/glycine-rich domain, which is interrupted by a predicted transmembrane sequence. This extended proline/glycine-rich region may be required for an unusual geometry of interaction with ligand or substrates. Discoidin I domains are also found in other proteins, including coagulation factors V and VIII, and may represent a class of domains that interact with specific cell surface molecules.
Johnson
J D
JD
Hormone Research Institute, University of California, San Francisco 94143-0534.
Edman
J C
JC
Rutter
W J
WJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000201:enzymology*; D002460:Cell Line; D004023:Dictyostelium / Q000235:genetics; D000070821:Discoidins; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D037102:Lectins / Q000235:genetics; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014407:Tumor Cells, Cultured
D000070821:Discoidins; D005656:Fungal Proteins; D037102:Lectins; D016376:Oligonucleotides, Antisense; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D011505:Protein-Tyrosine Kinases