The effect of adrenalectomy on the restraint stressed induced suppression of MHC class II expression by murine peritoneal macrophages.
Restraint stress suppresses the expression of MHC class II (I-A) glycoproteins by murine peritoneal macrophages. In order to evaluate the role of the pituitary adrenal axis, the effect of restraint stress on I-A expression by macrophages from adrenalectomized mice was evaluated. Adrenalectomy resulted in elevated levels of ACTH but abrogated the increase in corticosterone that results from restraint stress. The expression of I-A by macrophages from adrenalectomized mice was similar to that of sham operated mice or untreated mice. Adrenalectomy ameliorated the suppressive effects of restraint but the expression of I-A was below that of macrophages from adrenalectomized control mice. These results suggest that other factors in addition to corticosterone may effect I-A expression by murine peritoneal macrophages. A survey of other neuropeptides and catecholamines indicated that the addition of ACTH or epinephrine to macrophage cultures resulted in a suppression of I-A expression.
Department of Microbiology, Ohio State University, Columbus 43210.
B S
BS
Zwilling
D
D
Brown
N
N
Feng
J
J
Sheridan
D
D
Pearl
D000315:Adrenalectomy; D000324:Adrenocorticotropic Hormone / Q000502:physiology; D000818:Animals; D003345:Corticosterone / Q000502:physiology; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010913:Pituitary-Adrenal System / Q000503:physiopathology*; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000276:immunology / Q000503:physiopathology*
D000949:Histocompatibility Antigens Class II; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone