Cloning and sequence analysis of the human beta 1-adrenergic receptor 5'-flanking promoter region.
We present 3.1 kb of the nucleotide sequence from the 5'-flanking region of the human beta 1-adrenergic receptor gene. The first 1.0 kb upstream from the translational start site is composed of 72% G + C residues. The sequence was analyzed for the presence of transcriptional regulatory elements and contains putative thyroid hormone, glucocorticoid hormone and cAMP response elements. These putative hormone response elements support physiological evidence that thyroid and glucocorticoid hormones regulate beta 1AR function by affecting receptor expression in tissues such as heart and adipose, where beta 1-adrenergic receptors are important regulators of heart rate and lipolysis, respectively.
Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710.
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S
Collins
J
J
Ostrowski
R J
RJ
Lefkowitz
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D011943:Receptors, Adrenergic, beta / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D011943:Receptors, Adrenergic, beta