The polymerase chain reaction for detecting adenovirus DNA in formalin-fixed, paraffin-embedded tissue obtained post mortem.
The polymerase chain reaction (PCR) was used to detect adenovirus DNA in formalin-fixed, paraffin-embedded tissue obtained post mortem. Adenovirus DNA was successfully amplified from specimens of lung and liver from two patients with disseminated adenovirus infection confirmed by virus isolation, electron microscopy and/or immunohistochemistry. Negative results were obtained for specimens of lung from two patients with cytomegalovirus pneumonia. The specificity of the adenovirus PCR was confirmed by means of a digoxigenin-labelled probe generated in a separate PCR. Detection of viral nucleic acid by PCR in tissues obtained post mortem has considerable diagnostic potential.
Department of Pathological Sciences, Medical School, Manchester, U.K.
P C
PC
Turner
A S
AS
Bailey
R J
RJ
Cooper
D J
DJ
Morris
D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000382:microbiology*; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000328:Adult; D001344:Autopsy; D001483:Base Sequence; D002648:Child; D004279:DNA, Viral / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D015335:Molecular Probes; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D015335:Molecular Probes