Determination of morphine-3-glucuronide in human urine by capillary zone electrophoresis and micellar electrokinetic capillary chromatography.
Attempts to determine morphine-3-glucuronide (MO3G) by high-performance capillary electrophoresis and micellar electrokinetic capillary chromatography are reported. Using direct injection of urine, it was possible to achieve a limit of detection of about 20 micrograms/ml, which is poor compared with high-performance liquid chromatography and immunoassays. However, employing sample extraction with C8 cartridges, the presence of MO3G in urines that tested positive for opioids using a commercial enzyme-multiplied immunoassay technique could be successfully confirmed. The limit of detection with unambiguous identification of MO3G via spectral analysis was about 1 microgram/ml.
Department of Clinical Pharmacology, University of Bern, Switzerland.
P
P
Wernly
W
W
Thormann
D
D
Bourquin
R
R
Brenneisen
D002021:Buffers; D002845:Chromatography; D004563:Electrochemistry; D004586:Electrophoresis; D017248:Enzyme Multiplied Immunoassay Technique; D006801:Humans; D008823:Micelles; D009022:Morphine Derivatives / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection
D002021:Buffers; D008823:Micelles; D009022:Morphine Derivatives; C018108:morphine-3-glucuronide