Differential expression of c-erbA mRNAs in the developing cerebellum and cerebral cortex of the rat.
Expression of c-erbA genes, which encode thyroid hormone receptors, has been shown to be tissue and age specific. The differential regulation of thyroid hormone receptor mRNAs in rat brain was examined in the present study using Northern hybridization techniques. Ontogenic profiles of two known mRNAs for rat thyroid hormone receptors (rTR), rTR alpha and rTR beta, were determined in the rat cerebellum and cerebral cortex. Three bands of approximately 6, 5 and 2.6 kb in size were detected at 1, 6, 12 and 21 days of age (preweanling period) and in the adult, in both brain regions using a rTR alpha-common DNA probe. The 2.6-kb band was expressed at much higher levels than either the 5- or 6-kb band at all ages tested. The 6- and 5-kb bands were expressed at similar levels throughout the preweanling period in the cerebellum, but only at 1 and 6 days of age in the cerebral cortex. The 6-kb transcript was expressed at a much lower level than the 5-kb mRNA by postnatal day 12 in the cerebral cortex. A similar change in the relative level of 6- to 5-kb mRNA expression was noted in cerebellar mRNA isolated from adult animals. The overall expression of rTR alpha mRNA was less in the adult cerebellum and cerebral cortex compared to that in the preweanling period. The rTR beta transcripts were present in very low levels in the cerebral cortex and were undetectable in the cerebellum. These results are consistent with the fact that the developing brain is more sensitive to thyroid hormone than the mature rat brain.(ABSTRACT TRUNCATED AT 250 WORDS)
Sherer
T T
TT
Pharmacology/Toxicology Program, College of Pharmacy, Washington State University, Pullman 99164-6510.
Sylvester
S R
SR
Bull
R J
RJ
D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism*; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D011988:Receptors, Thyroid Hormone / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone