Strict peptide length is not required for the induction of cytotoxic T lymphocyte-mediated antiviral protection by peptide vaccination.
Sendai virus nuclear protein peptides of different lengths were titrated in peptide vaccination experiments. We observed that peptide length was not important in inducing cytotoxic T lymphocyte-mediated protective immunity in vivo against a challenge with a lethal dose of virus. These results suggest that long peptides are trimmed in vivo to peptides that fit into the groove of major histocompatibility complex class I molecules. In addition several adjuvants were screened for their effectiveness in peptide vaccination protocols. Incomplete Freund's adjuvant and Titermax turned out to be useful, whereas alum was much less effective.
Kast
W M
WM
Dept. of Immunohematology and Blood Bank, University Hospital, Leiden, The Netherlands.
Brandt
R M
RM
Melief
C J
CJ
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D015395:Histocompatibility Antigens Class I / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments; D014764:Viral Proteins; D014765:Viral Vaccines