Promoter region of the rat phospholipase C-gamma 1 gene.
We have cloned the upstream region of the rat phospholipase C-gamma 1 gene and characterized its promoter activity. The 5'-upstream sequence is highly rich in GC, enriched with CpG dinucleotides and lacks a TATA motif. This sequence also contains many putative binding sites for regulatory proteins. Primer extension and RNase protection assay demonstrated a single transcriptional start site located at 103 nucleotides upstream of the ATG start codon. The transcriptional activities of various 5'-deleted fragments, fused with chloramphenicol acetyltransferase gene, were examined after transfection into C2C12 myoblast. Multiple positive and negative regulatory sites were observed.
Lee
S J
SJ
Department of Life Science, Pohang Institute of Science and Technology, Korea.
Ryu
S H
SH
Suh
P G
PG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000201:enzymology; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D015698:Genomic Library; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D007527:Isoenzymes; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D010727:Phosphoric Diester Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase