Direct enantiospecific HPLC bioanalysis of (R,S)-atenolol on a chiral stationary phase.
The simultaneous determination of the enantiomers of the beta1-selective adrenergic antagonist atenolol in human plasma and urine is described. After an alkaline pre-extraction atenolol is extracted from biological material at pH 12.3 using dichloromethane/propan-2-ol. The separation of the underivatized enantiomers is achieved by high-performance liquid chromatography on a chiral stationary phase (Chiralcel OD, cellulose tris-3,5-dimethylphenylcarbamate, coated on silica gel) with fluorimetric detection. (--)-)S)-Pindolol is used as an internal standard. The detection limits of 5 ng/ml enantiomer in plasma and 50 ng/ml enantiomer in urine are sufficient for pharmacokinetic studies after therapeutic doses.
Department of Pharmacology, Johann Wolfgang Goethe University, Frankfurt am Main, Germany.
B
B
Kofahl
D
D
Henke
E
E
Mutschler
D001262:Atenolol / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D015203:Reproducibility of Results; D013237:Stereoisomerism
D001262:Atenolol