Effects of protein kinase C activation on norepinephrine-induced phosphatidylinositide hydrolysis in intact rat aorta.
The purpose of this study was to investigate the role of protein kinase C in the regulation of alpha 1-adrenoceptor-mediated phosphatidylinositide hydrolysis in intact vascular smooth muscle. Phorbol myristate acetate (0.1 and 1 microM) and staurosporine inhibited and potentiated, respectively, norepinephrine-induced inositol phosphate formation in intact rat aorta. In contrast, 30 microM prostaglandin F2 alpha, which activated protein kinase C to a similar magnitude as 1 microM phorbol myristate acetate, was without effect on norepinephrine-induced inositol phosphate formation. These results suggest that protein kinase C activated in response to physiologic agonists, but not in response to phorbol esters, may be compartmentalized within the smooth muscle cell.
Bazan
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Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, OH 45267-0575.
Campbell
A K
AK
Rapoport
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RM
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism; D015237:Dinoprost / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006868:Hydrolysis; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology*; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000470:Alkaloids; D010716:Phosphatidylinositols; D015237:Dinoprost; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate; D009638:Norepinephrine