Use of an immunoperoxidase technique to detect equine herpesvirus-1 antigen in formalin-fixed paraffin-embedded equine fetal tissues.
An indirect immunoperoxidase (IP) procedure using the avidin-biotin-peroxidase complex detection technique was developed to detect viral equine herpesvirus-1 (EHV-1) antigen in formalin-fixed paraffin-embedded tissues from aborted equine fetuses. The procedure was applied to liver, lung, and other tissues from 20 cases of confirmed or suspected EHV-1-induced abortions. Specific staining was observed in tissue sections from EHV-1-infected fetuses. Positive IP staining was present in tissues of 7 cases that were also positive by fluorescent antibody (FA) and virus isolation (VI) and that had typical histologic lesions. There was no IP staining in 7 cases that had no histologic lesions and negative FA and VI results. Five cases had typical histologic lesions and positive results in only 1 laboratory test; 3 were positive by VI and 2 by FA. Liver of 1 case was positive by IP, but tissues were too autolytic for other tests to be evaluated.
Veterinary Diagnostic Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
P C
PC
Schultheiss
J K
JK
Collins
J
J
Carman
D000034:Abortion, Veterinary; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D005260:Female; D005333:Fetus; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification*; D006736:Horses; D007124:Immunoenzyme Techniques; D008099:Liver / Q000382:microbiology* / Q000473:pathology; D008168:Lung / Q000382:microbiology / Q000473:pathology; D009336:Necrosis; D010920:Placenta / Q000382:microbiology / Q000473:pathology; D011247:Pregnancy
D000956:Antigens, Viral