Detection of genes that are differentially expressed during mouse embryogenesis by genetic trapping strategies.
OBJECTIVE
Our objective was to identify novel genes that are expressed in temporally and spatially restricted patterns during mouse embryonic development and organogenesis.


METHODS
Two genetic trapping reporter constructs that lack transcriptional regulatory sequences were introduced independently into transcriptionally active gene loci by electroporation into mouse embryonic stem cells. Patterns of host gene-reporter construct expression were investigated in differentiated embryonic stem cells, embryoid bodies, and chimeric embryos at various stages of development.


RESULTS
Three patterns of host gene-reporter construct expression were observed from the developmental analysis of four vector-integrated cell lines. Reporter expression patterns reflecting developmental regulation, constitutive activity, and developmental inactivation of the host genes were observed.


CONCLUSIONS
Two vector-integrated gene loci from cell lines CCE-1C1 and D3-B44 have expression patterns not previously described by genetic trapping. Molecular characterization of these interrupted genes will shed light on their developmental function.
Johnson
M D
MD
Section on Developmental Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland.
Mahon
K A
KA
D000818:Animals; D002478:Cells, Cultured; D004742:Enhancer Elements, Genetic / Q000502:physiology; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL / Q000196:embryology* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000502:physiology; D013234:Stem Cells; D001616:beta-Galactosidase / Q000096:biosynthesis
D001616:beta-Galactosidase