Increment of the cyclin D1 mRNA level in TPA-treated three human myeloid leukemia cell lines: HEL, CMK and HL-60 cells.
To study the involvement of cyclins in cell-cycle progression, changes of mRNA levels for three G1 cyclins (cyclin C, D1 and E) and cyclin A were studied in a leukemia cell line, HEL cells, before and after incubation with 12-O-tetradecanoylphorbol-13-acetate (TPA). Unexpectedly, the cyclin D1 mRNA level markedly increased in the HEL cells when the cells were growth-arrested by TPA, while the amounts of cyclin E and A mRNAs decreased to an almost undetectable level in HEL cells after incubation with TPA. The similar marked increment of the cyclin D1 mRNA level was observed in other leukemia cell lines, CMK and HL-60 cells, after incubation with TPA. The cyclin C mRNA was not detected in HEL cells before and after incubation with TPA.
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
N
N
Akiyama
H
H
Sasaki
O
O
Katoh
T
T
Sato
H
H
Hirai
Y
Y
Yazaki
T
T
Sugimura
M
M
Terada
D001483:Base Sequence; D015152:Blotting, Northern; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D019938:Cyclin D1; D016213:Cyclins / Q000096:biosynthesis*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004938:Esophageal Neoplasms; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D015513:Oncogene Proteins / Q000096:biosynthesis*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D016213:Cyclins; D015345:Oligonucleotide Probes; D015513:Oncogene Proteins; D012333:RNA, Messenger; D019938:Cyclin D1; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide