High-performance liquid chromatographic method for the determination of 3-methylindole (skatole) and indole in adipose tissue of pigs.
A rapid method for the determination of skatole (3-methylindole) and indole in adipose tissue of pigs by reversed-phase high-performance liquid chromatography has been developed. Tissue samples were melted in a microwave oven, and 100 microliters of the liquid fat were dissolved in 1 ml of n-hexane and extracted with acetonitrile-water (75:25, v/v). Portions of 100 microliters of the solution were used for chromatographic analysis. Elution was performed on a reversed-phase column with a mobile phase composed of acetic acid and isopropanol (70:30, v/v). A fluorescence detector was used for quantification. The detection limit was 4 ng/g fat. The mean recoveries of added amounts of skatole and indole were 98.9 and 93.8%, respectively. The mean coefficients of variation were: inter-assay, 6.6% (skatole) and 8.8% (indole); intra-assay, 4.2% (skatole) and 2.9% (indole). Mean skatole concentrations in fat samples from boars (40 ng/g; n = 349) were not significantly higher than those from barrows (24 ng/g; n = 98).
Dehnhard
M
M
University of Hohenheim, Stuttgart, Germany.
Claus
R
R
Hillenbrand
M
M
Herzog
A
A
D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007211:Indoles / Q000032:analysis*; D008297:Male; D009919:Orchiectomy; D011786:Quality Control; D012862:Skatole / Q000032:analysis*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013552:Swine
D007211:Indoles; D012996:Solutions; D012862:Skatole