A survey of clinical isolates of Enterobacteriaceae using a series of DNA probes for aminoglycoside resistance genes.
DNA probes specific for the aminoglycoside adenylyltransferase ANT(2"), the aminoglycoside phosphotransferases APH(3')-I and APH(3')-II, and the aminoglycoside acetyltransferases AAC(3)-I, AAC(3)-V and AAC(6')-I were used to screen 151 clinical isolates for the presence of these resistance determinants. The two most common resistance genes in the study isolates were those encoding ANT(2") and APH(3')-I. The phenotypic pattern of aminoglycoside resistance, as determined by a modified disk diffusion test correlated well with the genotypes of the organisms as established using DNA probes, although strains carrying the ANT(2") gene could express one of several phenotypes.
Seattle Veterans Affairs Medical Center, Washington 98108.
R K
RK
Flamm
K L
KL
Phillips
F C
FC
Tenover
J J
JJ
Plorde
D000123:Acetyltransferases / Q000235:genetics*; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015342:DNA Probes; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D019868:Kanamycin Kinase; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D013045:Species Specificity
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015342:DNA Probes; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; C056532:aminoglycoside N(6')-acetyltransferase; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D009713:Nucleotidyltransferases; C020565:gentamicin 2''-nucleotidyltransferase