Sugar composition of dietary fibre and short-chain fatty acid production during in vitro fermentation by human bacteria.
The aim of the present study was to assess the relationship between the disappearance of dietary fibre sugars and the production of individual short-chain fatty acids (SCFA). The bacterial degradation of five dietary fibres whose sugars were quantified was investigated in vitro using a human faecal inoculum. Involvement of the main fibre sugars in SCFA production was evaluated by a stepwise multiple linear regression. The results show first that the nature and chiefly the associations between the fibre sugars were key variables in the fermentability. Second, the nature and the amounts of SCFA produced were closely related to the in vitro fermentation of the main sugars available: uronic acids seemed to be principally involved in the production of acetic acid whereas the production of propionic acid could be promoted by the fermentation of glucose and, to a lesser extent, by that of xylose and arabinose. Xylose tended to have a greater impact than uronic acids and glucose on the production of butyric acid. Thus, it would be possible to predict which SCFA could be specifically produced during the fermentation of a fibre, as far as the chemical composition and structure of this fibre are known.
Salvador
V
V
INRA, Laboratory of Nutrition and Applied Technology, Nantes, France.
Cherbut
C
C
Barry
J L
JL
Bertrand
D
D
Bonnet
C
C
Delort-Laval
J
J
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D001419:Bacteria / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile / Q000096:biosynthesis*; D005285:Fermentation / Q000502:physiology*; D006801:Humans; D011422:Propionates / Q000378:metabolism
D000085:Acetates; D002087:Butyrates; D004040:Dietary Carbohydrates; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D011422:Propionates; D020148:Butyric Acid; C029658:propionic acid; D019342:Acetic Acid