Regulation of human papillomavirus type 16 (HPV-16) transcription by loci on the short arm of chromosome 11 is mediated by the TATAAAA motif of the HPV-16 promoter.
The human papillomavirus type 16 (HPV-16) enhancer-promoter is virtually inactive in normal human diploid fibroblasts, but active in human fibroblasts with a deletion in the short arm of one chromosome 11 (del-11 cells). Since the HPV-16 enhancer with the simian virus 40 promoter is active in both cell types, the target for chromosome 11-regulated HPV-expression is likely to be located in the HPV-16 early promoter region (nucleotides 57 to 112). We show here that DNA-protein complexes formed with an HPV-16 promoter fragment are quantitatively different in del-11 cell and diploid cell extracts. This quantitative difference detected in band shift experiments disappeared upon mutation of the HPV-16 TATAAAA box to TATTTAT. This mutation also strongly reduced the activity of the HPV-16 enhancer-promoter in del-11 cells. These results indicate that TATA-binding proteins are involved in the chromosome 11-mediated regulation of HPV-16 gene expression.
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
P H
PH
Smits
H L
HL
Smits
R P
RP
Minnaar
J
J
ter Schegget
D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11 / Q000502:physiology*; D004279:DNA, Viral / Q000235:genetics* / Q000502:physiology; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology*; D016385:TATA Box / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*
D004279:DNA, Viral