Apoptosis induced by tumor necrosis factor in cells chronically infected with feline immunodeficiency virus.
Tumor necrosis factor alpha (TNF-alpha) induced morphologic changes such as chromatin condensation and cell shrinkage in a feline fibroblastic cell line (CRFK) chronically infected with feline immunodeficiency virus (FIV) but not in uninfected CRFK cells. DNA extracted from TNF-alpha-treated CRFK cells infected with FIV showed a ladder of nucleosomal DNA, indicating that this cytocidal effect by TNF-alpha was due to programmed cell death, or apoptosis. These findings may have implications for understanding the pathogenesis of FIV infection and for the design of specific therapeutic strategies for AIDS in humans as well as cats.
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
K
K
Ohno
T
T
Nakano
Y
Y
Matsumoto
T
T
Watari
R
R
Goitsuka
H
H
Nakayama
H
H
Tsujimoto
A
A
Hasegawa
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002415:Cats; D002460:Cell Line; D048429:Cell Size; D002470:Cell Survival; D002843:Chromatin; D002908:Chronic Disease; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects*; D004249:DNA Damage; D016181:Feline Acquired Immunodeficiency Syndrome / Q000473:pathology*; D005347:Fibroblasts / Q000166:cytology / Q000382:microbiology; D016089:Immunodeficiency Virus, Feline / Q000254:growth & development / Q000472:pathogenicity*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014779:Virus Replication
D002843:Chromatin; D014409:Tumor Necrosis Factor-alpha