[Serological property of perchloric acid-soluble glycoprotein fractions of human meconium].
Perchloric acid-soluble fractions (PASFs) were separated, in yields of 87.0-151.9 mg per 1.0 g of wet meconium sample, from six samples of human meconium, and identified as glycoproteins having 41.2-74.1% carbohydrate by chemical composition analysis. Six PASFs reacted with anti-A, -B, -Lea and -Leb sera, but did not react with anti-M and -N sera. These PASFs alos reacted with many lectins such as DBA, UEA-I, Vicia graminea lectin (VGA), Vicia unijuga lectin (VUA), Con A, WGA, RCA-I, PHA-E, SBA, SJA, and influenza A and B viruses. The serological results show that six PASFs contained VGA or VUA-binding (Vgu) glycoproteins as new oncofetal substances, but did not contain M and N blood group substances.
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
N
N
Uchide
K
K
Ohyama
T
T
Yamakawa
H
H
Watanabe
S
S
Hirakawa
K
K
Yoneyama
S
S
Ohkuma
D000596:Amino Acids / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007231:Infant, Newborn; D037102:Lectins; D008470:Meconium / Q000737:chemistry*; D010472:Perchlorates; D037121:Plant Lectins; D012995:Solubility
D000596:Amino Acids; D006023:Glycoproteins; D037102:Lectins; D010472:Perchlorates; D037121:Plant Lectins; C042242:Vicia lectins