Molecular cloning of a novel rat G-protein-coupled receptor gene expressed prominently in lung, adrenal, and liver.
A gene and cDNA (named G10d) encoding a novel member of the G-protein-coupled receptor (GCR) superfamily has been isolated from a rat genomic library and a rat liver cDNA library by tandom polymerase chain reaction (PCR) amplification and hybridization screening. Comparison of the sequence of the encoded protein to other members of the rhodopsin family of the GCR superfamily suggests that this new member is a peptide receptor. The G10d gene is present as a single copy in the rat genome and its translational open reading frame is intronless. Northern analysis demonstrates a wide and uneven distribution of G10d mRNA in the adult rat, with the highest mRNA abundance in lung, liver and adrenal gland extracts.
Department of Pharmacology, University of Virginia School of Medicine, Charlottesville 22908.
J K
JK
Harrison
C M
CM
Barber
K R
KR
Lynch
D000311:Adrenal Glands / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D058265:Receptors, Adrenomedullin; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D058265:Receptors, Adrenomedullin; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C081830:adrenomedullin receptor, rat; D004247:DNA; D019204:GTP-Binding Proteins