Intracellular accumulation of cytosine arabinoside in murine normal and neoplastic lymphocytes following their exposure to sodium 2-mercaptoethanesulphonate.
The effect of mesna on intracellular accumulation of cytosine arabinoside (Ara-C) in murine normal and neoplastic lymphocytes was studied. Simultaneous exposure of cells to mesna at concentrations ranging from 0.25 to 1.0 mM and 3H-Ara-C (40.0 nM) resulted in a strong inhibition of Ara-C uptake in normal lymphocytes. Under the same experimental conditions, mesna did not affect the Ara-C uptake in neoplastic cells (cultured L5178Y mouse leukaemia cells and neoplastically transformed thymus cells). It was found that the inhibitory effect of mesna was not cell cycle-dependent, since mesna reduced the Ara-C uptake in both normal quiescent and PHA-stimulated cells. We therefore concluded that mesna may selectively reduce Ara-C uptake by normal cells in vitro.
Jastrzebski
Z
Z
Department of Pharmacology, Institute of Drug Research and Control, Warsaw, Poland.
Czyzewska-Szafran
H
H
Remiszewska
M
M
Côte-Rozanes
C
C
D000818:Animals; D002478:Cells, Cultured; D003561:Cytarabine / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007940:Leukemia L5178 / Q000378:metabolism*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism*; D008297:Male; D015080:Mesna / Q000494:pharmacology*; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D010835:Phytohemagglutinins; D003561:Cytarabine; D015080:Mesna