The monomer covalently closed linear replicative form DNA of Aleutian disease parvovirus is infectious after transfection into permissive cells.
The recently described monomer covalently closed linear replicative form DNA (Mccl RF DNA) of Aleutian disease parvovirus (ADV) is an infectious intermediate of the viral DNA replication cycle. Transfection of highly purified Mccl RF DNA into susceptible feline kidney cells (CCC clone 81 cells) resulted in viral DNA replication, expression of viral proteins and synthesis of infectious progeny virus. Mccl RF DNAs generated under permissive (32 degrees C) or non-permissive (37 degrees C) conditions were shown to be biologically indistinguishable. The accumulation of the Mccl RF DNA form at the non-permissive temperature in vitro strongly resembles that in bone marrow cells of naturally infected mink and may reflect one mechanism contributing to virus persistence of ADV in vivo.
Truyen
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James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Schelp
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Löchelt
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Kaaden
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D000454:Aleutian Mink Disease Virus / Q000235:genetics* / Q000502:physiology; D000818:Animals; D002415:Cats; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000502:physiology*; D014162:Transfection; D014779:Virus Replication
D004279:DNA, Viral