Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene.
The RNA genome of tobacco etch potyvirus (TEV) was engineered to express bacterial beta-glucuronidase (GUS) fused to the virus helper component proteinase (HC-Pro). It was shown previously that prolonged periods (approximately 1 month) of TEV-GUS propagation in plants resulted in the appearance of spontaneous deletion variants. Nine deletion mutants were identified by nucleotide sequence analysis of 40 cDNA clones obtained after polymerase chain reaction amplification. The mutants were missing between 1,741 and 2,074 nucleotides from TEV-GUS, including the sequences coding for most of GUS and the N-terminal region of HC-Pro. This region of HC-Pro contains determinants involved in helper component activity during aphid transmission, as well as a highly conserved series of cysteine residues. The deletion variants were shown to replicate and move systemically without the aid of a helper virus. Infectious viruses harboring the two largest HC-Pro deletions (termed TEV-2del and TEV-7del) were reconstructed by subcloning the corresponding mutated regions into full-length DNA copies of the TEV genome. Characterization of these and additional variants derived by site-directed mutagenesis demonstrated that deletion of sequences coding for the HC-Pro N-terminal domain had a negative effect on accumulation of viral RNA and coat protein. The TEV-2del variant possessed an aphid-nontransmissible phenotype that could be rescued partially by prefeeding of aphids on active HC-Pro from another potyvirus. These data suggest that the N-terminal domain of HC-Pro or its coding sequence enhances virus replication or genome expression but does not provide an activity essential for these processes. The function of this domain, as well as a proposed deletion mechanism involving nonhomologous recombination, is discussed.
Dolja
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VV
Department of Biology, Texas A&M University, College Station 77843-3258.
Herndon
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KL
Pirone
T P
TP
Carrington
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000382:microbiology; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003545:Cysteine / Q000032:analysis; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial; D016679:Genome, Viral; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification; D010947:Plants, Toxic; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014026:Nicotiana / Q000382:microbiology; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D005966:Glucuronidase; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus; D003545:Cysteine