Establishment of a human small cell lung carcinoma cell line carrying amplification of c-myc gene and chromosomal translocation of t(3p;6p) and t(12q;17p).
A transplantable tumor and an in vitro culture cell line (GK-T3) were established from metastatic liver tissue of human small cell lung carcinoma (SCLC). Southern blot analysis revealed about 30-fold amplification of c-myc gene in the tumor cells in liver, xenografts, and in vitro cell line. The degree of c-myc amplification was essentially conserved through serial passages in nude mice and cultivation in vitro. The level of c-myc mRNA was significantly increased in these cells. Cytogenetically, numerical and complex structural abnormalities were observed in GK-T3 cells, including t(3p;6p), t(12q;17p), two homogeneously staining regions (hsrs) and several double minutes (dmins). These results suggest that activation of c-myc gene and alteration of gene(s) around these chromosomal breakpoints may play a role in tumorigenesis of GK-T3 SCLC.
First Department of Medicine, Faculty of Medicine, Tokyo Medical and Dental University.
A
A
Kato
T
T
Kamiyama
S
S
Yamaguchi
K
K
Sudo
S
S
Mori
M
M
Sekiguchi
N
N
Aoki
M
M
Shibuya
D000368:Aged; D000818:Animals; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D002893:Chromosomes, Human, Pair 3; D002896:Chromosomes, Human, Pair 6; D004273:DNA, Neoplasm / Q000235:genetics; D005784:Gene Amplification / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm