The rat beta 3-adrenergic receptor gene contains an intron.
We report that the rat beta 3-adrenergic receptor (beta 3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta 3-AR were both deduced from genomic DNA sequences. Given the heterogeneity at the C-termini of the otherwise highly similar rat, mouse and human sequences, we discuss the intriguing possibility that the beta 3-AR gene of the latter two species also contain an intron near the extremity of the open reading frame. A beta-adrenergic receptor (beta-AR) cDNA we have cloned from rat colonic tissue which has a sequence essentially identical to that previously reported for the rat adipose beta 3-AR cDNA [(1991) J. Chem. 266, 24053], encodes the spliced version of the beta 3-AR.
Laboratoire de Biologie Moleculaire, Sanofi Elf Biorecherches, Labege, France.
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Bensaid
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Kaghad
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Rodriguez
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Le Fur
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017403:In Situ Hybridization; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000235:genetics*
D003062:Codon; D011943:Receptors, Adrenergic, beta; D004247:DNA