Novel fluoride-stimulated dichlorofluorescin dye oxidation pathway in human leukemia cell lines.
NaF stimulation produces a strong dichlorofluorescin (DCFH) dye oxidation signal in HL-60 and PLB-985 human myeloid leukemia cells. This response is comparable in differentiated and undifferentiated cells and occurs in undifferentiated cells when only small amounts of extracellular superoxide or hydrogen peroxide are generated. A comparable response was also observed in human TK-6 B-lymphoblastoid cells. NaF-stimulated DCFH oxidation appears to occur via a pathway that is distinct from respiratory burst oxidant-mediated DCFH oxidation. This alternative pathway may play a role in the normal physiology of myeloid and lymphoid cells, and may represent a confounding variable when DCFH is used to quantify the respiratory burst activity of human phagocytic cells.
Department of Pathology SM-30, University of Washington, Seattle 98195.
G A
GA
Maresh
R J
RJ
Monnat
D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation; D004126:Dimethylformamide / Q000494:pharmacology; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007700:Kinetics; D007951:Leukemia, Myeloid; D015473:Leukemia, Promyelocytic, Acute; D010084:Oxidation-Reduction; D012969:Sodium Fluoride / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D005452:Fluoresceins; D013481:Superoxides; C037631:2',7'-dichlorofluorescein; D004126:Dimethylformamide; D012969:Sodium Fluoride; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate