Production of interleukin-5 and granulocyte/macrophage colony-stimulating factor by T cells of patients with bronchial asthma in response to Dermatophagoides farinae and its relation to eosinophil colony-stimulating factor.
We investigated the effects of Dermatophagoides farinae (Df) and interleukin (IL)-2 on the release of eosinophil colony-stimulating factor (Eo-CSF) activity from mononuclear cells (MNC) and lymphocytes of patients with bronchial asthma (BA) who were sensitive to Df to clarify its relationship with IL-5 and granulocyte/macrophage colony-stimulating factor (GM-CSF). MNC and T cells of patients cultured with IL-2 and Df released Eo-CSF activity. These Eo-CSF activities were partially inhibited by anti-IL-5 and anti-GM-CSF antibodies. In 11 of 15 cases studied, MNC from patients produced GM-CSF in response to IL-2. In four of 15 cases studied, MNC from patients produced GM-CSF in response to Df. On culture with IL-2 or Df, the releases of IL-5 into the medium by MNC from individual patients varied. The results indicate that in BA responsiveness of lymphocytes to Df is increased, and suggest that IL-5 and GM-CSF produced by T cells play a role in the induction of eosinophilia and the pathogenesis of BA.
Third Department of Internal Medicine, University of Tokushima, School of Medicine, Japan.
T
T
Kamei
T
T
Ozaki
K
K
Kawaji
K
K
Banno
T
T
Sano
M
M
Azuma
T
T
Ogura
D000818:Animals; D001249:Asthma / Q000276:immunology*; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D004804:Eosinophils / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D015848:Interleukin-5 / Q000096:biosynthesis*; D008925:Mites / Q000276:immunology*; D009000:Monocytes / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D015848:Interleukin-5; D010835:Phytohemagglutinins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor