Role of Porphyromonas gingivalis-derived fibroblast-activating factor in bone resorption.
A 24-kDa protein was isolated from the outer membrane vesicles of Porphyromaonas gingivalis W50. This protein, referred to as fibroblast-activating factor (FAF), was examined for its bone-resorptive ability by the rat long-bone assay and the mouse bone marrow cell culture system. FAF resulted in a significant release of 45Ca from cultured bones, as well as the formation of tartrate-resistant acid phosphatase-positive monocytes. These cultures were compared with control cells. FAF therefore might be considered a significant bacterially expressed protein which could affect and modulate the resorption or destruction of tissue and alveolar bone in the local periodontal environment.
Dentistry for the Handicapped, Osaka University Dental Hospital, Japan.
J
J
Mihara
T
T
Yoneda
S C
SC
Holt
D000818:Animals; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D001862:Bone Resorption / Q000139:chemically induced*; D010450:Endopeptidases; D005260:Female; D018093:Gelatinases; D006133:Growth Substances / Q000633:toxicity*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008813:Mice, Inbred ICR; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D011247:Pregnancy; D051381:Rats; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D010450:Endopeptidases; D018093:Gelatinases; D006133:Growth Substances; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha