Cyclooxygenase and lipoxygenase arachidonate metabolites synthesized by mouse peritoneal macrophages: in vitro effect of N-phenyllinoleamide from toxic oil samples.
N-phenyllinoleamide (NPLA) has been detected as extraneous compound in adulterated cooking oils associated with a unique epidemic disease known as the Toxic Oil Syndrome (TOS). In this communication we report on the action of NPLA on the endogenous cyclooxygenase and lipoxygenase arachidonate metabolism. Results show that mouse peritoneal macrophages (MPM) exposed to 1 mM NPLA for 2 h undergo significant increases of 6-keto prostaglandin F1a, prostaglandin E2, leukotriene B4, 12- and 15-hydroxyeicosatetraenoic acids. MPM prelabelled with 3H-AA showed an enhanced release when exposed to NPLA. Thus, it is concluded that NPLA potentiates AA release from cell membrane phospholipids and the subsequent cyclooxygenase and lipoxygenase oxidative metabolism of this precursor to various eicosanoids. This is in agreement with the implication of peroxidative process mediated by fatty acids anilides in TOS.
Molecular Pathology Unit, CID-CSIC, Barcelona, Spain.
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Bioque
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Vargas
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Bulbena
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Roselló-Catafau
Gelpi E
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000704:Analysis of Variance; D000813:Anilides / Q000633:toxicity*; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001937:Brassica; D002478:Cells, Cultured; D005229:Fatty Acids, Monounsaturated; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism; D008041:Linoleic Acids / Q000633:toxicity*; D008084:Lipoxygenase / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity; D010938:Plant Oils / Q000506:poisoning; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism; D011863:Radioimmunoassay; D000074262:Rapeseed Oil; D013931:Thromboxanes / Q000378:metabolism
D000813:Anilides; D005229:Fatty Acids, Monounsaturated; D006893:Hydroxyeicosatetraenoic Acids; D008041:Linoleic Acids; D010938:Plant Oils; D011453:Prostaglandins; D000074262:Rapeseed Oil; D013931:Thromboxanes; C039294:linoleylanilide; D007975:Leukotriene B4; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases