Plaquing efficiency of herpes simplex virus type 1 with a defined agar overlay.
Plaque assay was performed on herpes simplex type 1, using Vero, BHK-21, Hep-2 and LLCMK2 cell lines. The plaquing efficiency was optimal when 60 mm dishes containing Vero cells received an inoculum of 200 microliters with an adsorption time of 90 min. The overlay contained agar and tissue culture medium supplemented with extra vitamins, DEAE-Dextran and magnesium chloride. Virus titer was increased by two fold and plaques were clearly visible within three days of incubation. This modified method was reproducible and is being used routinely in our research and diagnostic laboratories.
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, Riyadh, Kingdom of Saudi Arabia.
Y M
YM
Siddiqui
M N
MN
al-Ahdal
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D015169:Colony Count, Microbial; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D003637:DEAE-Dextran; D006801:Humans; D015636:Magnesium Chloride; D018139:Simplexvirus / Q000254:growth & development*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D010948:Viral Plaque Assay / Q000379:methods*
D003470:Culture Media; D015636:Magnesium Chloride; D003637:DEAE-Dextran