Detection of bovine leukemia virus RNA in serum using the polymerase chain reaction.
A method was developed for detecting bovine leukemia virus (BLV) RNA in serum samples using a pair of primers from the BLV polymerase gene in the polymerase chain reaction (PCR). The PCR was able to detect 3800-7600 molecules of BLV RNA. At this level of sensitivity eleven pools of adult and one fetal bovine serum appeared free from BLV contamination.
Poon
H
H
Connaught Product Development Centre, Connaught Laboratories Limited, Willowdale, Ont., Canada.
Jimenez
E
E
Jacobs
R M
RM
Song
Z
Z
Jefferson
B
B
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics; D016583:Enzootic Bovine Leukosis / Q000517:prevention & control; D005069:Evaluation Studies as Topic; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary*; D012367:RNA, Viral / Q000097:blood / Q000235:genetics*; D012680:Sensitivity and Specificity; D014765:Viral Vaccines / Q000302:isolation & purification
D004279:DNA, Viral; D012367:RNA, Viral; D014765:Viral Vaccines