Purification and characterization of a Sindbis virus-induced peptide which stimulates its own production and blocks virus RNA synthesis.
The antiviral activity described by Riedel and Brown (J. Virol. 29, 51-60) has been purified to homogeneity. A study of the effects of this pure peptide on mosquito cell growth and virus replication has been undertaken. We have found that treatment of noninfected Aedes albopictus cells with this antiviral peptide results in a temporary arrest of cell division accompanied by acquisition of virus resistance and the constitutive production of the antiviral protein.
Luo
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Cell Research Institute, University of Texas, Austin 78713-7640.
Brown
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D000330:Aedes / Q000166:cytology; D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D006224:Cricetinae; D007113:Immunity, Innate; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D012367:RNA, Viral / Q000096:biosynthesis; D012845:Sindbis Virus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity; D014158:Transcription, Genetic / Q000187:drug effects*; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D010455:Peptides; D012367:RNA, Viral