Xenoxins, a family of peptides from dorsal gland secretion of Xenopus laevis related to snake venom cytotoxins and neurotoxins.
Three new, highly similar peptides from the skin secretion of Xenopus laevis have been purified and analyzed by mass spectrometry and Edman degradation. The 66-amino-acid peptides, termed xenoxin-1, -2, and -3, contain 8 cysteines and show similarity to snake venom cytotoxins and short neurotoxins. Assignment of two out of four disulfide bonds suggests a tertiary structure similar to that of cytotoxins and short neurotoxins. A cDNA encoding pre-xenoxin-1 was isolated from a X. laevis skin cDNA library. The nucleotide sequence predicts the synthesis of a precursor with a signal peptide followed by the sequence of the mature peptide. Xenoxin-1 and -2 lack alpha-neurotoxic activity, have apparently no antibacterial activity, are low in general toxicity as tested in mice, and have no effect on blood coagulation as measured in a Factor VIII procoagulant activity test. Potential functions of xenoxins as well as evolutionary aspects are discussed.
Transgène S. A., Strasbourg, France.
H V
HV
Kolbe
A
A
Huber
P
P
Cordier
U B
UB
Rasmussen
B
B
Bouchon
M
M
Jaquinod
R
R
Vlasak
E C
EC
Délot
G
G
Kreil
D029404:Activin Receptors; D000595:Amino Acid Sequence; D000664:Amphibian Venoms / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003603:Cytotoxins / Q000737:chemistry*; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012910:Snake Venoms / Q000737:chemistry*; D014101:Torpedo; D014982:Xenopus laevis
D000664:Amphibian Venoms; D003603:Cytotoxins; D009498:Neurotoxins; D011956:Receptors, Cell Surface; D012910:Snake Venoms; C082510:xenoxin 1; C082511:xenoxin 2; D004247:DNA; D029404:Activin Receptors