Characterization of the karyotype of the tench (Tinca tinca L.) and analysis of its chromosomal heterochromatic regions by C-banding, Ag-staining, and restriction endonuclease banding.
Beginning with a description of the conventional Giemsa-stained karyotype of the tench (Tinca tinca L.), the structure and variability of the chromosomal heterochromatic regions in this cyprinid species were analyzed by means of C-, silver-, and restriction endonuclease banding. Silver staining revealed active nucleolus organizer regions (NORs) on the secondary constriction of chromosome pair 3. Constitutive heterochromatin was associated with NOR regions detected by C-banding. Restriction endonuclease digestion with AluI, TaqI, and HaeIII induced specific banding patterns that allowed identification of homologous chromosome pairs and revealed features about the sequence composition of several chromosomal heterochromatic regions and of the NOR-associated heterochromatin.
Departamento de Zootecnia, Genética y Mejora Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
J A
JA
Padilla
J L
JL
Fernández-García
A
A
Rabasco
M
M
Martínez-Trancón
I
I
Rodriguez de Ledesma
J J
JJ
Pérez-Regadera
D000818:Animals; D001399:Azure Stains; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure*; D003530:Cyprinidae / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005260:Female; D006570:Heterochromatin / Q000648:ultrastructure*; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D016622:Silver Staining
D001399:Azure Stains; D006570:Heterochromatin; D004262:DNA Restriction Enzymes