Normal distribution of alpha-1-adrenoceptors in the rat spinal cord and its modification after noradrenergic denervation: a quantitative autoradiographic study.
The distribution of alpha 1 (alpha 1)-adrenoceptors along the different segments of the spinal cord (cervical, thoracic, lumbar, and sacral) of normal rats has been studied by quantitative autoradiography using the specific alpha 1-antagonist [3H]Prazosin as a ligand. In addition, the influence of noradrenergic (NA) denervation [obtained either by complete transection of the spinal cord at vertebrae level T8-T9 or by selective lesion of NA spinal cord system carried out by intracisternal injection of 6-hydroxydopamine (6-OHDA)] on eventual variations of alpha 1-adrenoceptor density at spinal cord target cells was studied in parallel. In control rats, the quantitative analysis of alpha 1-adrenoceptor densities revealed a widespread distribution of these receptors along all segments of the spinal cord with a similar pattern in the various subregions of gray matter studied. This distribution of alpha 1-adrenoceptors was quite well correlated with the distribution of NA terminals, when referring to previous descriptions by immunohistochemistry. After 6-OHDA lesion, as well as caudally to the transection, a significant increase of alpha 1-adrenoceptor densities was observed in all spinal subregions thus evidencing supersensitivity. These results suggest that NA may act in the spinal cord, at least partly, via alpha 1-adrenoceptors and that the expression of these receptors could be influenced by NA dysfunction, as demonstrated here through the effects observed in lesioned animals.
INSERM U.318, LAPSEN, Département des Neurosciences Cliniques et Biologiques, Grenoble, France.
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Roudet
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Savasta
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Feuerstein
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D003714:Denervation / Q000379:methods; D008297:Male; D009638:Norepinephrine / Q000502:physiology*; D016627:Oxidopamine / Q000494:pharmacology; D011224:Prazosin / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D012016:Reference Values; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D014018:Tissue Distribution
D011942:Receptors, Adrenergic, alpha; D016627:Oxidopamine; D009638:Norepinephrine; D011224:Prazosin