Characterization of a full-length cDNA encoding a bovine four tandem-repeat ubiquitin.
Screening of a bovine seminal vesicle cDNA library in lambda gt11 with monospecific anti-ubiquitin IgGs yielded two independent clones pUF4AA and pUD4AA. Sequence analysis of pUF4AA revealed a coding region for a polyubiquitin with four tandem-repeats. We observed a replacement of serine (133) by phenylalanine in the second ubiquitin repeat. The deduced amino acid sequence of pUD4AA, however, possessed the typical serine (133) residue. This serine-phenylalanine replacement could result from allelic polymorphism.
Max-Planck-Institut für biophysikalische Chemie, Department of Molecular Biology, Göttingen, Germany.
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D008297:Male; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012669:Seminal Vesicles / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics*
D014452:Ubiquitins; D004247:DNA