Preliminary crystallographic studies on the D15 5' to 3' exonuclease from phage T5.
The D15 exonuclease from phage T5 has been crystallized from 35% (w/v) ammonium sulfate by the hanging drop vapor diffusion technique. The crystals grow in tetragonal space group P4(1)22 or P4(3)22 with cell dimensions a = b = 79.2 A and c = 138.0 A. The crystals diffract to 2.5 A and are suitable for X-ray structure determination.
Ceska
T A
TA
EMBL, Heidelberg, Germany.
Sayers
J R
JR
Eckstein
F
F
Suck
D
D
D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D005090:Exodeoxyribonucleases; C079044:T5 D15 exonuclease