The binding of diazepam in the mitochondria of Tetrahymena pyriformis as detected by quantitative high resolution autoradiography.
Tritiated diazepam accumulates mainly in the mitochondria of the unicellular Tetrahymena. This is the case in both a single (the first encounter) and a repeated (one day or a week after the first) administration of the drug. When imprinting of Tetrahymena by diazepam (the first encounter) is followed a week later by the administration of the labelled drug, the membranes of the vesicles, too, show the appearance of label. Regarding the studies presented here, the unicellular Tetrahymena also contain diazepam receptors in the mitochondria as suggested for cells of higher rank animals.
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
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D000818:Animals; D001345:Autoradiography; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D003975:Diazepam / Q000032:analysis / Q000378:metabolism*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D011963:Receptors, GABA-A / Q000032:analysis / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000378:metabolism* / Q000648:ultrastructure
D011963:Receptors, GABA-A; D003975:Diazepam