Uptake and subcellular distribution of azithromycin in human phagocytic cells. Demonstration of the antibiotic in neutrophil polymorphonuclear leucocytes and monocytes by autoradiography and electron microscopy.
Azithromycin (CAS 83905-01-5), a new azalide antibiotic, reached high concentrations in alveolar macrophages obtained from patients. Uptake of [14C] azithromycin by macrophages resulted in an intracellular/extracellular concentration ratio of approximately 634 after 90 min incubation. Electron microscopic autoradiography provided visual evidence for the marked uptake and localization of [3H] azithromycin in human neutrophils and monocytes isolated from healthy volunteers. Quantitative analysis of the autoradiographs showed the distribution of azithromycin over the various compartments of the phagocytic cells.
Wildfeuer
A
A
Department of Pathology, University of Ulm, Fed. Rep. of Germany.
Reisert
I
I
Laufen
H
H
D001345:Autoradiography; D017963:Azithromycin; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009000:Monocytes / Q000378:metabolism / Q000648:ultrastructure; D009504:Neutrophils / Q000378:metabolism / Q000648:ultrastructure; D010586:Phagocytes / Q000378:metabolism* / Q000648:ultrastructure
D004917:Erythromycin; D017963:Azithromycin