Cytogenetic studies on human liver cancer cell lines.
Karyotyping of human liver cancer cell lines and chromosome in situ hybridization of hepatitis B virus (HBV) DNA was performed in order to elucidate the possible mechanism of hepatocarcinogenesis from evidence of chromosomal changes and HBV integration patterns. HepG2-2 and HepG2-5 cell lines were HepG2 cells experimentally transfected with HBV DNA; the HCC36 cell line was derived from a hepatocellular carcinoma containing integrated HBV DNA from a Taiwanese patient. HepG2 cells, a hepatoblastoma cell line without HBV DNA integration, served as negative control. In HepG2-2, HepG2-5, and HCC36 cells, multiple integrations of HBV DNA were observed by in situ hybridization and hybridization signals occurred preferentially on certain chromosomes: 2, 5, 10, 11, 18; 7, 10, 13, 17, 18; and 4, 6, 11, 12q+, 18; respectively. In addition, a strong correlation between chromosomal changes and HBV integration was noticed in HCC36 cells, especially at chromosome 12q+.
Hepatitis Research Center, National Taiwan University Hospital, Taipei, Republic of China.
H L
HL
Chen
T S
TS
Chiu
P J
PJ
Chen
D S
DS
Chen
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000382:microbiology; D002869:Chromosome Aberrations / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics* / Q000382:microbiology; D014407:Tumor Cells, Cultured
D004279:DNA, Viral