Morphological changes and reorganization of actinfilaments in human myeloid leukemia cells induced by a novel protein phosphatase inhibitor, tautomycin.
A protein phosphatase inhibitor, tautomycin induces blebs on the surface of human myeloid leukemia K562 cells within 10 min. In this paper we examined the cytoskeleton of tautomycin-treated cells. In the presence of tautomycin, cells with blebs turned into segmented forms with smooth surfaces after 15 min and into smooth round shapes without microprotuberance after 60 min. Observation with fluorescence microscopy showed that F-actin detached from the plasma membrane at the site of the blebs. Further treatment with tautomycin induced the accumulation of F-actin at the segmentation centers. Under electron microscopy, an electron dense ring-structure was detected at the segmentation center. Tautomycin did not induce major changes of the microtubule network although F-actin accumulated near the microtubule organizing center. The amount of F-actin increased in tautomycin-treated cells. These results indicate that the morphological changes are induced by reorganization of actinfilaments.
Kurisaki
T
T
Department of Agricultural Chemistry, University of Tokyo, Japan.
Magae
J
J
Nagai
K
K
Hirata
A
A
Kusaka
I
I
Shinji
H
H
Morikawa
M
M
Yoshida
T
T
Isono
K
K
Yamasaki
M
M
D008841:Actin Cytoskeleton / Q000737:chemistry* / Q000648:ultrastructure*; D000199:Actins / Q000032:analysis* / Q000648:ultrastructure; D000935:Antifungal Agents / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D015638:Cytochalasin D / Q000494:pharmacology; D006801:Humans; D007382:Intermediate Filaments / Q000737:chemistry / Q000648:ultrastructure; D007951:Leukemia, Myeloid / Q000473:pathology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry / Q000648:ultrastructure; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D011714:Pyrans; D013141:Spiro Compounds; D014407:Tumor Cells, Cultured
D000199:Actins; D000935:Antifungal Agents; D011714:Pyrans; D013141:Spiro Compounds; C053079:tautomycin; D015638:Cytochalasin D; D010749:Phosphoprotein Phosphatases