Hematopoietic recovery from host progenitors with normal karyotype devoid of Philadelphia chromosome in a patient with CML after allogeneic BMT.
A male patient with CML received a BMT from his sister and developed chronic GVHD. The host-origin normal karyotype (46,XY) was identified for the first time in the 60th month after BMT. Detection of Y-chromosome-specific DNA in BM and peripheral blood (PB) showed that all BM samples obtained 6 months from BMT were positive for Y-specific DNA, while PB became positive in the 60th month after BMT. The BCR-ABL mRNA derived from leukemic cells was detected in the 36th month post-BMT, but not in the 60th month or thereafter. Fluorescence in situ hybridization revealed that 1.5% and 0.6% in BM and PB cells were Y-positive in the 70th month post-BMT, respectively. DNA analysis of hematopoietic progenitor colonies revealed 1 of 42 erythroid colonies to be host derived. These results indicate that host-origin hematopoietic cells survive chronic GVHD, while the Ph1 clone was eliminated.
Second Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan.
T
T
Okamoto
A
A
Kanamaru
H
H
Wada
Y
Y
Takemoto
E
E
Kakishita
T
T
Hashimoto
J
J
Furuyama
K
K
Nagai
D000328:Adult; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D002908:Chronic Disease; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology / Q000235:genetics; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000648:ultrastructure; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000601:surgery*; D008297:Male; D008969:Molecular Sequence Data; D010677:Philadelphia Chromosome; D012333:RNA, Messenger / Q000235:genetics; D011828:Radiation Chimera; D014019:Tissue Donors
D004273:DNA, Neoplasm; D012333:RNA, Messenger