Acute and early effects of aldosterone on Na-K-ATPase activity in Madin-Darby canine kidney epithelial cells.
The time course and mechanism of early effects of aldosterone on renal Na-K-adenosinetriphosphatase (Na-K-ATPase) activity and number of units were studied in MDCK cells. Aldosterone induced a time- and dose-dependent stimulation of Na-K-ATPase activity. The stimulatory effect of aldosterone on activity and number of pump units increased progressively and was inhibited by spironolactone. In presence of cycloheximide, the stimulatory effect of aldosterone on activity and number of catalytic sites persisted to the same extent until 30 min and decreased by 20% after 60 min. In these cells, dimethylamiloride addition during preincubation abolished the aldosterone-induced stimulation in Na-K-ATPase activity up to 60 min. In contrast, furosemide addition did not alter the effect of aldosterone on Na-K-ATPase activity. The present study demonstrates an early effect of aldosterone on Na-K-ATPase activity that can be separated into the following two successive periods: 1) increase in pump number due to insertion of presynthetized units secondary to Na entry through an amiloride-sensitive apical pathway; and 2) an increase in pump number by de novo protein synthesis.
Département de Physiologie, Faculté de Médecine, Necker Enfants Malades, Paris, France.
M
M
Shahedi
K
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Laborde
L
L
Bussières
C
C
Sachs
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology / Q000378:metabolism; D005665:Furosemide / Q000494:pharmacology; D007668:Kidney / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D008985:Monensin / Q000494:pharmacology; D010042:Ouabain / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013997:Time Factors
D000450:Aldosterone; D010042:Ouabain; D005665:Furosemide; D008985:Monensin; D003513:Cycloheximide; D000254:Sodium-Potassium-Exchanging ATPase