The human rod photoreceptor cGMP phosphodiesterase beta-subunit. Structural studies of its cDNA and gene.
cDNA clones encoding the beta-subunit of the photoreceptor cGMP phosphodiesterase (PDE) were isolated from a human retina library and their sequence was determined. The encoded polypeptide consists of 854 amino acid residues with a calculated molecular mass of 98,416 Da. Alignment of the deduced amino acid sequence with the earlier analysed alpha-, beta- and alpha'-subunits of bovine and mouse PDEs demonstrates a high homology. Two overlapping recombinant lambda phage clones containing 26 kb of the human PDE beta-subunit gene were isolated from the genomic library. A total nucleotide sequence of exons 4-22 of the PDE beta-subunit gene was established which completely corresponded to the cDNA structure. According to sequence analysis no potential possibility for alternative splicing of the beta-subunit gene was observed between exons 20 and 21 which led to the formation of the beta'-subunit as described for mouse PDE. Polymerase chain reaction (PCR) experiments also confirm the absence of the PDE beta'-subunit in human retina.
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
N V
NV
Khramtsov
E A
EA
Feshchenko
V A
VA
Suslova
B E
BE
Shmukler
B E
BE
Terpugov
T V
TV
Rakitina
N V
NV
Atabekova
V M
VM
Lipkin
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D010727:Phosphoric Diester Hydrolases; D010786:Photoreceptor Cells / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D010727:Phosphoric Diester Hydrolases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; C517431:PDE6B protein, human; C517438:Pde6b protein, mouse