Clinical significance of HIV DNA in polymorphonuclear neutrophils from patients with HIV infection.
HIV DNA was detected by the polymerase chain reaction technique in polymorphonuclear neutrophils (PMNs) in 11 of 37 (29.7%) HIV-infected patients. A detectable level of HIV DNA in PMNs was more common in symptomatic than asymptomatic HIV infected patients (46.7% and 18.2%, respectively; p < 0.05). HIV DNA in PMNs was detected most frequently in patients with recurrent bacterial pneumonia or Pneumocystis carininii pneumonia. An association between HIV DNA in PMNs and a low CD4/8 ratio as well as high levels of immunoglobulins in the sera was noted. Detectable HIV DNA was found more frequently in patients with neutropenia than in those with a normal level of neutrophils in peripheral blood (44.4% and 28.0%, respectively; p < 0.05). These data suggest that infection of PMNs by HIV may be associated with PMN impairment during HIV infection.
Gabrilovich
D I
DI
Laboratory of AIDS Epidemiology and Prevention, Central Institute for Epidemiology, Moscow, Russia.
Vassilev
V
V
Nosikov
V V
VV
Serebrovskaya
L V
LV
Ivanova
L A
LA
Pokrovsky
V V
VV
D000328:Adult; D016516:CD4-CD8 Ratio; D004279:DNA, Viral / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology*; D010587:Phagocytosis; D011020:Pneumonia, Pneumocystis / Q000276:immunology / Q000382:microbiology; D011023:Pneumonia, Staphylococcal / Q000276:immunology / Q000382:microbiology; D016133:Polymerase Chain Reaction; D016897:Respiratory Burst; D013481:Superoxides
D004279:DNA, Viral; D013481:Superoxides