Expression of hepatitis A virus poly(U) polymerase in the periplasmic space of Escherichia coli.
A cDNA containing almost all of the hepatitis A virus (HAV) P3 sequences was expressed as a fusion protein with Protein A. A novel poly(U) polymerase activity was detected in the periplasmic space of Escherichia coli cells transformed with this plasmid, and this activity showed many of the expected properties of a picornavirus 3Dpol. A number of HAV-specific polypeptides were detected in these cells, and it is unclear which of these was responsible for the polymerase activity.
Department of Biochemistry, University of Bath, Claverton Down, U.K.
A J
AJ
Wolstenholme
J P
JP
Coates
W
W
Douglass
D004926:Escherichia coli / Q000201:enzymology; D006507:Hepatovirus / Q000201:enzymology*; D009713:Nucleotidyltransferases / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
D011993:Recombinant Fusion Proteins; D009713:Nucleotidyltransferases; C022428:poly U polymerase