MyoD and regulation of prostaglandin H synthase.
MM14 myoblasts, in contrast to their differentiation defective variant (DD-1) cells, do not synthesize detectable levels of prostaglandins or of the initial enzyme in the pathway of prostaglandin synthesis, prostaglandin H synthase (PGHS) but do exhibit readily detectable level of PGHS mRNA (Steiner, S., et al., 1991, Exp. Cell Res. 192, 643). These findings suggest a possible relationship between the myogenic phenotype and the synthesis of prostaglandins. This relationship was examined in the current study by analysis of the effect of transfection of DD-1 cells with a MyoD expression vector (termed MyoDD-1 cells) on expression of MyoD and synthesis of prostaglandins. Proliferating MyoDD-1 cells express readily detectable levels of MyoD protein and mRNA and exhibit markedly diminished levels of PGHS protein and prostaglandins. In contrast, serum-deprived MyoDD-1 cells express little MyoD mRNA or protein and exhibit a readily detectable level of PGHS protein despite having only a slightly higher PGHS mRNA abundance compared to growing MyoDD-1 cells. These studies are consistent with the hypothesis that MyoD expression contributes to the inhibition of prostaglandin synthesis.
Wolf
J R
JR
T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington 40506-02256.
Hu
Y
Y
Hirschhorn
R R
RR
Steiner
S M
SM
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002874:Chromosome Mapping; D015870:Gene Expression / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005822:Genetic Vectors; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D017570:MyoD Protein; D009206:Myocardium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D009124:Muscle Proteins; D017570:MyoD Protein; D012333:RNA, Messenger; D011451:Prostaglandin-Endoperoxide Synthases