Enzymatic hydrolysis of reconstituted dimyristoylphosphatidylcholine-apo A-I complexes.
Apolipoproteins share a common structural feature, their interaction with phospholipids. It is believed that amphipathic helical sequences enable apolipoproteins to bind to lipid bilayer and to form discoidal particles of defined dimensions. While the knowledge of the apo A-I sequence and secondary structure has been used to make predictions about its mode of association with lipids, the available experimental data necessary to propose a precise model of these discoidal structures are still limited. An important step in our understanding of these structures would be to identify the apolipoprotein lipid-associated domains. Proteolysis of apo A-I-DMPC reconstituted HDL (rHDL) and free apo A-I is used here to identify lipid-protected domains of apo A-I. Free cleaved peptides were separated from rHDL associated peptides by density gradient centrifugation. The lipid-associated peptides were further analyzed by SDS-PAGE and transferred by Western blot to a ProBlott membrane for sequencing. Cleavage occurred at residue 43 with proteinase K, 46 with trypsin and residue 47 or 48 with pronase. A large domain from about residue 45 to the C-terminal remains highly protected against hydrolysis eventhough it contains several bonds susceptible to proteolytic cleavage. No protected fragments were detected by SDS-PAGE after enzymatic cleavage of free apo A-I in identical experimental conditions.
Laboratoire de Chimie Physique des Macromolécules aux Interfaces, Free University of Brussels, Belgium.
L
L
Lins
S
S
Piron
K
K
Conrath
B
B
Vanloo
R
R
Brasseur
M
M
Rosseneu
J
J
Baert
J M
JM
Ruysschaert
D000595:Amino Acid Sequence; D016632:Apolipoprotein A-I / Q000737:chemistry* / Q000648:ultrastructure; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D019286:Endopeptidase K; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D011402:Pronase / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*
D016632:Apolipoprotein A-I; D010446:Peptide Fragments; D012697:Serine Endopeptidases; D014357:Trypsin; D019286:Endopeptidase K; D011402:Pronase; D004134:Dimyristoylphosphatidylcholine