Compatibility of Tat and Rev transactivators in the primate lentiviruses.
Primate immunodeficiency viruses carry a unique set of transacting regulator genes, which are essential for viral replication. The exchangeability of these Tat and Rev transactivators derived from viruses of the four major subgroups identified to date was assessed in transient transfection and infection assay systems. The human immunodeficiency virus type 1 (HIV-1), a major causative virus of human AIDS, efficiently activated the other viruses. In contrast, the tat and rev gene products of HIV-2, SIVAGM (virus of the African green monkey), and SIVMND (virus of the mandrill) did not fully transactivate the HIV-1. In particular, the rev of HIV-1 was not substantially replaced by those of the other viruses. The result that HIV-1 is distinct from the other immunodeficiency viruses with respect to the compatibility of two transactivators gives a firm functional basis for the unique phylogenetic position of HIV-1.
Institute for Virus Research, Kyoto University, Japan.
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H
Sakai
J
J
Sakuragi
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S
Sakuragi
M
M
Kawamura
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A
Adachi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015694:Gene Products, rev / Q000502:physiology*; D015696:Gene Products, tat / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D015696:Gene Products, tat; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus