The mouse retinoid-X receptor-gamma gene: genomic organization and evidence for functional isoforms.
Retinoid-X receptors (RXRs) are a subfamily of the steroid/thyroid nuclear receptor superfamily. There are three RXR genes: alpha, beta, and gamma, each with a distinct expression pattern and chromosomal location. In this study we describe the genomic organization of the mouse RXR gamma gene, identify a second mouse (m) RXR gamma isoform, and map the exons of the two mRXR gamma isoforms on the gene. The new mRXR gamma isoform, called gamma 2, has a unique 5'-untranslated region, and is expressed highly in both cardiac and skeletal muscles, but very little in the liver, while the mRXR gamma 1 is expressed in the brain and muscle. The gamma 2 isoform is also a functional receptor and can transactivate the retinoid response elements of the apolipoprotein-AI and cellular retinol-binding protein-II genes. The mRXR gamma gene spans at least 50 kilobases of DNA on mouse chromosome 1 and has nine introns ranging from 107 basepairs to more than 30 kilobases. From the genomic separation of the two unique 5'-ends of the mRXR gamma isoforms, it is suggested that this gene may have two promoters, similar to those described for the three RAR genes. The results of this study along with those of previously published studies suggest that there may be more than two isoforms of mRXR gamma.
Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710.
Q
Q
Liu
E
E
Linney
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics; D004247:DNA / Q000737:chemistry; D005091:Exons; D015870:Gene Expression / Q000187:drug effects; D007438:Introns; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D018168:Receptors, Retinoic Acid; D011988:Receptors, Thyroid Hormone / Q000235:genetics; D015183:Restriction Mapping; D047488:Retinoid X Receptors; D014157:Transcription Factors; D014212:Tretinoin / Q000494:pharmacology
D002352:Carrier Proteins; D011956:Receptors, Cell Surface; D018168:Receptors, Retinoic Acid; D011988:Receptors, Thyroid Hormone; D047488:Retinoid X Receptors; D014157:Transcription Factors; D014212:Tretinoin; D004247:DNA