Forensic DNA typing by the solid-phase minisequencing method.
We describe a method for DNA-typing, in which a panel of biallelic markers are detected by the solid-phase minisequencing method (Syvänen et al., 1990). This method identifies single nucleotide variations in DNA fragments amplified by the PCR. Determination of the panel of 12 markers selected in this study proved to be an efficient and reliable method for forensic identification of individuals. We also introduce a novel approach for rapid determination of allele frequencies by quantitative analysis of pooled DNA samples.
Department of Human Molecular Genetics, National Public Health Institute, Helsinki, Finland.
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Syvänen
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Sajantila
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Lukka
D000483:Alleles; D016172:DNA Fingerprinting / Q000379:methods* / Q000706:statistics & numerical data; D004276:DNA, Satellite / Q000235:genetics; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008297:Male; D010334:Paternity; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA / Q000379:methods
D004276:DNA, Satellite; D005819:Genetic Markers