Characterization of the UL10 gene product of herpes simplex virus type 1 and investigation of its role in vivo.
On the basis of predicted amino acid sequence characteristics, herpes simplex virus type 1 gene UL10 is thought likely to encode a membrane protein with eight potential transmembrane regions. Previously, a protein with an apparent M(r) 47,000 on SDS-PAGE was identified as a product of this gene. Here we have further characterized this protein, and show that it is modified by N-linked glycosylation, associates with membranes from infected cells, and is a component of the virus particle. It is not essential for virus growth in tissue culture. To investigate its role in vivo a deletion mutant lacking the majority of the UL10 open reading frame was constructed (UL10-del). The in vitro growth properties of this virus were consistent with previous studies; it grew to give slightly lower yields than wild-type and revertant viruses, and had no apparent temperature-sensitive or host range phenotype. In vivo, in a mouse model, UL10-del was capable of establishing a latent infection, although it was impaired for growth at the periphery, and for spread to and/or growth within the nervous system relative to wild-type or revertant viruses.
MRC Virology Unit, University of Glasgow, U.K.
C A
CA
MacLean
L M
LM
Robertson
F E
FE
Jamieson
D000818:Animals; D005260:Female; D017353:Gene Deletion; D005814:Genes, Viral / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry; D006031:Glycosylation; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008566:Membranes / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000737:chemistry* / Q000235:genetics / Q000472:pathogenicity; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics; D014771:Virion / Q000737:chemistry / Q000254:growth & development; D014774:Virulence; D014775:Virus Activation
D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C081689:UL10 protein, Human herpesvirus 1; D014764:Viral Proteins