Assay of nucleoside diphosphate kinase in microtiter plates using a peroxidase-coupled method.
A sensitive assay for nucleoside diphosphate kinase which utilizes microtiter plates is described. ATP, formed in the reaction between dGTP and ADP, is trapped by the glycerol kinase reaction. A colored compound is generated by glycerol-3-phosphate oxidase and peroxidase. This assay is useful for testing a large number of samples generated by chromatographic techniques or for screening purposes.
Lascu
I
I
Unité de Biochimie Cellulaire, Institut Pasteur, Paris, France.
LeBlay
K
K
Lacombe
M L
ML
Presecan
E
E
Véron
M
M
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000263:Adenylate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism; D003848:Deoxyguanine Nucleotides / Q000378:metabolism; D015226:Dinucleoside Phosphates / Q000494:pharmacology; D005991:Glycerol Kinase / Q000378:metabolism; D007700:Kinetics; D008832:Microchemistry / Q000379:methods; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*
D003848:Deoxyguanine Nucleotides; D015226:Dinucleoside Phosphates; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate; D000244:Adenosine Diphosphate; C029603:deoxyguanosine triphosphate; D000255:Adenosine Triphosphate; D010544:Peroxidases; D005991:Glycerol Kinase; D000263:Adenylate Kinase; D009701:Nucleoside-Diphosphate Kinase