Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage phi X174.
This report describes the isolation and characterization of second-site suppressors of a cold-sensitive (cs) external scaffolding protein, gpD, of bacteriophage phi X174. Seven genetically distinct suppressors were isolated. Six of them are located in gene F which encodes the major coat protein of the virus. The seventh is located in gene J which encodes the DNA-binding protein. A subset of the suppressors are trans-acting. These second-site suppressors do not exhibit allele specificity; they are able to suppress defects associated with a csD protein for which they were not selected. The initial characterization of the second-site suppressors and their locations within the major coat protein suggest that the mechanism of suppression may involve both structural and stoichiometric phenomena.
Department of Biological Sciences, University of Arkansas, Fayetteville 72701.
B A
BA
Fane
S
S
Shien
M
M
Hayashi
D000483:Alleles; D010584:Bacteriophage phi X 174 / Q000235:genetics*; D003080:Cold Temperature; D005814:Genes, Viral; D005816:Genetic Complementation Test; D009841:Oligonucleotides; D013489:Suppression, Genetic; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000235:genetics*
D009841:Oligonucleotides; D014764:Viral Proteins