Calf small intestine receptors for K99 fimbriated enterotoxigenic Escherichia coli.
Non-acid and acid glycolipids were isolated from the small intestine of a newborn calf and tested for the ability to bind Escherichia coli carrying K99 fimbriae. The bacteria did not bind to any of the non-acid glycolipids, whereas in the acid glycolipid fraction several gangliosides were detected which bind to K99 fimbriae. Gangliosides capable of binding K99 fimbriated E. coli were characterized as NeuGc-GM3, NeuGc-GM2, NeuGc-GD1a NeuAc-SPG and NeuAc-SPG. No binding was detected to NeuAc-GM3 and NeuGc-GM1.
Department of Medical Biochemistry, University of Gothenburg, Sweden.
S
S
Teneberg
P T
PT
Willemsen
F K
FK
de Graaf
K A
KA
Karlsson
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology*; D001427:Bacterial Toxins; D002240:Carbohydrate Sequence; D002417:Cattle; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000276:immunology / Q000502:physiology*; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism; D007421:Intestine, Small / Q000382:microbiology*; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000378:metabolism
D000954:Antigens, Surface; D001427:Bacterial Toxins; D004768:Enterotoxins; D005732:Gangliosides; D006017:Glycolipids; C031429:K99 antigen; D011956:Receptors, Cell Surface