Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting.
Well-defined Legionella pneumophila strains were analyzed by amplification of variable genomic regions with arbitrary and repeat sequence primers. Clinical and environmental outbreak-related isolates showed closely related amplicon patterns. Eleven strains of unrelated origins displayed 10 distinct patterns. Fingerprinting of L. pneumophila by polymerase chain reaction appeared to have the potential of being as epidemiologically useful as other genotypic methods.
Diagnostic Center SSDZ, Delft, The Netherlands.
A
A
van Belkum
M
M
Struelens
W
W
Quint
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction