Analysis of genetic markers by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR).
RAPD-PCR is a new technique that, starting from genomic DNA allows, with the use of a single primer of "random" base composition to amplify a variable number of sequences that can give important informations if analyzed for linkage studies, gene mapping or phylogenetic purposes. In order to detect the possible application of this simple way of DNA-fingerprinting in individual identification and in cell lineages characterization we analyzed human and non-human Primates DNA. Six different single primers of variable length were used and resulted in individual or specific electrophoretic patterns. As already reported we found a better resolution using "short" primers. The individual electrophoretic patterns obtained by RAPD-PCR can be a simple and reliable approach to DNA analysis.
Istituto ricerche cliniche, Firenze, Italy.
L
L
Sineo
R
R
Martini
G
G
Borghi
M
M
Failli
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D005784:Gene Amplification; D005819:Genetic Markers; D006801:Humans; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D005819:Genetic Markers; D004247:DNA