Enantioselective enzymatic biotransformation of 2'-deoxy-3'-thiacytidine (BCH 189) monitored by capillary electrophoresis.
Concentrations of the enantiomers of 2'-deoxy-3'-thiacytidine (BCH 189) have been monitored during enantiospecific biotransformation catalyzed by cytidine deaminase. This enzymatic reaction is monitored by capillary electrophoresis (CE) over a 51-h time period. The use of dimethyl-beta-cyclodextrin as a chiral electrolyte additive in a pH 2.3 phosphate buffer system gives enantiomeric separation with results for peak efficiencies, resolution, and analysis time of the order of 25,000 plates, > 1.5 and 30 min, respectively. The features of the CE method are compared with those of the previously favored chiral HPLC method.
Pharmaceutical Analysis Department, Glaxo Group Research, Ware, Hertfordshire, United Kingdom.
M M
MM
Rogan
C
C
Drake
D M
DM
Goodall
K D
KD
Altria
D000998:Antiviral Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003505:Cyclodextrins; D003564:Cytidine Deaminase / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D005069:Evaluation Studies as Topic; D019259:Lamivudine; D013237:Stereoisomerism; D016047:Zalcitabine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D047392:beta-Cyclodextrins
D000998:Antiviral Agents; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D019259:Lamivudine; D016047:Zalcitabine; D003564:Cytidine Deaminase; C031215:betadex