Effect of endotoxin on rat serum, lung and liver lipid peroxidation and on tissue metallothionein levels.
The effect of endotoxin on serum and lung and liver lipid peroxidation, as measured by thiobarbituric acid reactants (TBARs), as well as on lung and liver metallothionein (MT) has been studied in the rat. Endotoxin consistently increased serum and liver TBARs in a time-response manner. The increase in the serum preceded that in the liver, with peaks 3-6 h and 24 h after endotoxin administration, respectively. In contrast, lung TBARs levels did not increase regardless of the experimental approaches studied, suggesting that the rat is not a good model for the adult respiratory distress syndrome. Endotoxin increased both lung and liver MT levels in a time-response manner, although to a lesser degree in the former than in the latter tissue, indicating that this protein may have a significant role in the response of the organism to a septic insult.
Departamento de Biología Celular y Fisiología, Facultad de Ciencias Universidad Autónoma de Barcelona, Spain.
M
M
Giralt
T
T
Gasull
A
A
Blánquez
J
J
Hidalgo
D000818:Animals; D004195:Disease Models, Animal; D004333:Drug Administration Routes; D004731:Endotoxins / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D011654:Pulmonary Edema / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D004731:Endotoxins; D017392:Thiobarbituric Acid Reactive Substances; C044101:endotoxin, Escherichia coli; D008668:Metallothionein