Expression of insulin-like growth factor receptors I and II in normal human lung and in lung cancer.
Insulin-like growth factors are potent mitogenic factors in human lung cancer in vitro, acting via specific receptors. Using monoclonal antibodies we demonstrate the expression of insulin-like growth factor receptor I in bronchial epithelial cells of normal lung and in primary lung cancer (22/24 cases), being most prominent in squamous cell carcinoma. Electron microscopy on lung cancer cell lines reveals a distinct reaction pattern on the plasma membrane. Immunoreaction with a specific antibody directed against the insulin-like growth factor receptor II suggests a weak expression in primary lung cancer. Our findings underline the significance of the autocrine pathway of insulin-like growth factors in lung cancer.
Zentrum Innere Medizin, Abt. Haematologie/Onkologie, Marburg, Germany.
U
U
Kaiser
C
C
Schardt
D
D
Brandscheidt
E
E
Wollmer
K
K
Havemann
D000230:Adenocarcinoma / Q000737:chemistry; D001980:Bronchi / Q000737:chemistry* / Q000166:cytology; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry; D018288:Carcinoma, Small Cell / Q000737:chemistry / Q000648:ultrastructure; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000648:ultrastructure; D017526:Receptor, IGF Type 1 / Q000032:analysis*; D017527:Receptor, IGF Type 2 / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000648:ultrastructure
D017527:Receptor, IGF Type 2; D017526:Receptor, IGF Type 1