[A e year study of the electrophoretic RNA patterns of rotavirus in Valladolid].
BACKGROUND
To study the electrophoretic RNA patterns of human rotavirus (RV) during three years in Valladolid (Spain).


METHODS
From october 1986 to october 1989 we have studied the electrophoretic RNA patterns of RV in 104 fecal samples. Stools were first screened by a latex agglutination test (LA) and reactive samples were confirmed by a commercial enzymeimmunoassay (EIA) and electron microscopy (EM) (negative stain). Electropherotyping was made according to conventional methods.


RESULTS
96.2% of the samples studied yield a defined electropherotype. We have found 8 different types of patterns of genomic RV-RNA circulating in these three years. Two of them belonged to short pattern (C and E) and six to long pattern (A, B, D, F, G and H).


CONCLUSIONS
98.0% of the genomic RNA founded were of long pattern, from which pattern A was the most prevalent (72%) during the study, without a seasonal distribution. Pattern B was found in 20% of cases, while the rest were found only occasionally.
Ignacio Reguera
J
J
Facultad de Medicina, Universidad de Valladolid.
Ortiz de Lejarazu
R
R
Eirós
J M
JM
Bratos
M A
MA
Gonzalo
M P
MP
González
Z
Z
Pérez-Pascual
P
P
Rodríguez-Torres
A
A
D002648:Child; D002675:Child, Preschool; D004586:Electrophoresis; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D015995:Prevalence; D012367:RNA, Viral / Q000032:analysis / Q000145:classification*; D012401:Rotavirus / Q000235:genetics*; D012621:Seasons; D013030:Spain
D012367:RNA, Viral