A highly sensitive lactate editing technique for surface coil spectroscopic imaging in vivo.
A lactate editing sequence based on the use of spin-locking pulses with surface coils is described. The sequence retains more than 90% of the available lactate magnetization and suppresses at least 95% of the lipid signal in a single scan and ca. 99.5% after a simple two step cycle. The technique allows simultaneous observation of lactate and uncoupled metabolites in the range 2 to 3 ppm. The sequence was combined with two-dimensional spectroscopic imaging to obtain a lactate map of an ischemic rat brain in vivo.
Institute for Biodiagnostics, National Research Council of Canada, Ottawa, Ontario.
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D
Bourgeois
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Kozlowski
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D007089:Image Enhancement / Q000379:methods*; D007773:Lactates / Q000378:metabolism; D008055:Lipids; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008280:Magnetics; D008961:Models, Structural; D008962:Models, Theoretical; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D012815:Signal Processing, Computer-Assisted; D014867:Water
D007773:Lactates; D008055:Lipids; D010725:Phosphocreatine; D014867:Water; D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline