Biological receptors mediate anti-proliferative action of atrial natriuretic peptide in cultured mesangial cells.
The mechanism of anti-proliferative action of atrial natriuretic peptide (ANP) was examined in cultured mesangial cells. HS-142-1, a selective antagonist for ANP biological receptors, inhibited ANP-induced cellular cGMP accumulation and prevented ANP-induced inhibition of both serum-stimulated incorporation of 3H-thymidine and cell proliferation. Des-[Gln18, Ser19, Gly20, Leu21, Gly22]-ANP4-23-NH2 (C-ANP) failed to inhibit 3H-thymidine incorporation. These results indicate that anti-proliferative action of ANP is mediated by the biological receptors in cultured mesangial cells.
Third Department of Medicine, Shiga University of Medical Science, Japan.
M
M
Haneda
R
R
Kikkawa
D
D
Koya
K
K
Sakamoto
S
S
Nakanishi
Y
Y
Matsuda
Y
Y
Shigeta
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D001667:Binding, Competitive; D002455:Cell Division; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000378:metabolism; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011134:Polysaccharides / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017461:Receptors, Atrial Natriuretic Factor / Q000502:physiology*
C072551:HS 142-1; D011134:Polysaccharides; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP