High-performance liquid chromatographic and mass spectrometric identification of phosphocitrate synthesized by human kidney homogenate.
Two high-performance liquid chromatographic techniques for the analysis of phosphocitrate, a metabolite synthesized by the cell that is thought to play a role in a number of physiological as well as pathological events, have been developed. The mass spectra of the isolated compound obtained under fast-atom bombardment and collisionally activated decomposition mass-analysed ion kinetic energy conditions are also reported.
Romanello
M
M
Dipartimento di Biochimica, Università degli Studi di Trieste, Italy.
Michalsky
M
M
Stagni
N
N
Moro
L
L
Favretto
D
D
Traldi
P
P
D002851:Chromatography, High Pressure Liquid; D002951:Citrates / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D011188:Potassium / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment
D002951:Citrates; C014148:phosphocitrate; D011188:Potassium