CASTing for multicomponent DNA-binding complexes.
Degenerate oligonucleotides and polymerase chain reaction-based reiterative selection techniques have been used to define the consensus binding sites for an increasing number of transcription factors. The use of crude nuclear extracts rather than purified proteins permits multicomponent complexes to form, and allows the technique to generate information about the combinatorial interactions involved in gene regulation.
University of Texas Southwestern Medical Center, Dallas 75235.
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Wright
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Funk
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000378:metabolism; D018008:Myogenin; D016133:Polymerase Chain Reaction / Q000379:methods
D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D009124:Muscle Proteins; D018008:Myogenin; D004247:DNA