Polymerase chain reaction-mediated cloning and in vitro translation of the genes coding for the structural proteins of hog cholera virus.
After amplification by PCR, the 5' region of the genome of hog cholera virus (HCV) strain Alfort 187 was cloned and sequenced. The nucleotide and deduced amino acid sequences were compared with the ones of other pestiviruses. By in vitro translation experiments we were able to demonstrate the protease activity of the p 20 protein of HCV.
Instituut voor Molekulaire Biologie, Vrije Universiteit Brussel, Sint-Genesius-Rode, Belgium.
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G
Muyldermans
M C
MC
San Gabriel
A
A
Caij
A
A
De Smet
R
R
Hamers
D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010576:Pestivirus / Q000235:genetics; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism
D015678:Viral Structural Proteins; C083293:p20 protein, Classical swine fever virus