Movement of monovalent ions across the membranes of marginal cells of the stria vascularis in the guinea pig cochlea.
The resting potential and monovalent ions in the marginal cells and scala media were measured before and 20 min after the onset of anoxia using ion-sensitive microelectrodes. The resting potential of the marginal cells decreased from 62.7 to -2.4 mV. The K+ activity decreased from 77.7 to 53.2 mEq/l, while the Na+ activity increased from 2.6 to 24.7 mEq/l. The Cl- activity did not change significantly. In the scala media, the endocochlear potential decreased rapidly from 80.9 to -28.0 mV after the onset of anoxia. The K+ activity decreased from 119.0 to 96.5 mEq/l, the Na+ activity increased from 1.3 to 9.5 mEq/l and that of Cl- decreased from 127.0 to 115.1 mEq/l. The electrochemical gradients determined for each ion based on the ionic changes in the scala media and marginal cells, suggested the existence of an Na/K pump and Na-K-2Cl cotransport at the basolateral membrane of the marginal cells, and a rheogenic K pump and Na-K-2Cl transport at the luminal membrane of the marginal cells. The Na+ and K+ must be recycled at the basolateral membrane and luminal membrane of marginal cells, respectively.
Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
S
S
Komune
T
T
Nakagawa
K
K
Hisashi
T
T
Kimituki
T
T
Uemura
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D003053:Cochlear Duct / Q000378:metabolism / Q000502:physiology; D004563:Electrochemistry; D004710:Endolymph / Q000378:metabolism / Q000502:physiology; D006168:Guinea Pigs; D000860:Hypoxia / Q000097:blood / Q000378:metabolism / Q000503:physiopathology; D016623:Ion Pumps; D017136:Ion Transport / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013316:Stria Vascularis / Q000166:cytology / Q000378:metabolism* / Q000502:physiology
D002712:Chlorides; D016623:Ion Pumps; D012964:Sodium; D011188:Potassium