Detection of the excited singlet state of a deprotonated, reduced flavin.
The excited singlet state of a deprotonated, reduced flavin [1,5-dihydro-N(3)-carboxymethyllumiflavin] in aqueous solution at pH 8 has been detected by laser flash photolysis. The broad absorption band maximized at approximately 490 nm (epsilon = 9.9 x 10(3) M-1 cm-1). The lifetime of the transient was found to be 100 +/- 15 ps. The lifetime was not affected by the presence of pyrimidine dimers, which would be monomerized under these conditions. A longer-lived transient, tentatively identified as the solvated electron, was also detected. The neutral reduced flavin did not give a detectable transient.
Faculty of Science, Health and Medical Studies, North East Wales Institute, Deeside, Clwyd, UK.
P F
PF
Heelis
R F
RF
Hartman
S D
SD
Rose
D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism; D005415:Flavins / Q000737:chemistry / Q000528:radiation effects*; D010084:Oxidation-Reduction; D010777:Photochemistry; D010782:Photolysis; D011522:Protons; D011740:Pyrimidine Dimers / Q000378:metabolism / Q000528:radiation effects; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000528:radiation effects*
D005415:Flavins; D011522:Protons; D011740:Pyrimidine Dimers; D011838:Radiation-Sensitizing Agents; C083078:1,5-dihydro-N(3)-carboxymethyllumiflavin; D004255:Deoxyribodipyrimidine Photo-Lyase