Detection of anti-Fya, anti-S and anti-Jka in relation to the genotypes of the panel red cells: report of a U.K. NEQAS survey. U.K. National External Quality Assessment Scheme.
Three antibody-containing samples (anti-Fya, anti-S and anti-Jka), each at two dilutions, were distributed to U.K. hospitals and transfusion centres together with an antibody screening panel comprising red cells homozygous for the corresponding antigens. Participants in the study subjected the samples to their routine antibody screening procedures using both their own antibody screening panels and the screening panel provided. A within-group comparison of those participants using their own screening panels having a heterozygous expression of the antigens, with the same participants when using the screening panel provided, showed for five of the samples a greater detection rate in routine antibody screening procedures when using the panel provided, having homozygous expression of the corresponding antigens. The sixth sample, the most potent, was detected equally using both panels. The difference in overall detection rate is statistically significant (chi-square test, 2P < 0.001). The study shows that the use of red cells presumptively homozygous for Fya, S and Jka improved the detection of the corresponding antibodies.
National Institute for Biological Standards and Control, South Mimms, Hertfordshire, England.
P K
PK
Phillips
C M
CM
Whitton
D001788:Blood Grouping and Crossmatching / Q000592:standards*; D004375:Duffy Blood-Group System / Q000235:genetics / Q000276:immunology*; D005188:False Negative Reactions; D005838:Genotype; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D007667:Kidd Blood-Group System / Q000235:genetics / Q000276:immunology*; D008951:MNSs Blood-Group System / Q000235:genetics / Q000276:immunology*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004375:Duffy Blood-Group System; D007518:Isoantibodies; D007667:Kidd Blood-Group System; D008951:MNSs Blood-Group System