Branched-chain-amino-acid biosynthesis in plants: molecular cloning and characterization of the gene encoding acetohydroxy acid isomeroreductase (ketol-acid reductoisomerase) from Arabidopsis thaliana (thale cress).
Towards the goal of gaining a better understanding of the molecular mechanisms controlling branched-chain-amino-acid biosynthesis in plants, we have isolated, sequenced and characterized a gene encoding acetohydroxy acid isomero-reductase (ketol-acid reductoisomerase) from Arabidopsis thaliana (thale cress). Comparison between the acetohydroxy acid isomeroreductase cDNA and the genomic sequence has allowed us to determine the exon structure of the coding region. The isolated acetohydroxy acid isomeroreductase gene is distributed over approx. 4.5 kbp and contains nine introns (79-347 bp). The transcriptional start site was found to be 52 bp upstream of the translational initiation site. Southern-blot analysis of A. thaliana genomic DNA shows that the acetohydroxy acid isomeroreductase is encoded by a single-copy gene.
Dumas
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Unité Mixte CNRS/Rhône-Poulenc (Unité associée au Centre National de la Recherche Scientifique, U. M. 41), Lyon, France.
Curien
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DeRose
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RT
Douce
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D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D006860:Hydrogen Bonding; D007438:Introns; D050540:Ketol-Acid Reductoisomerase; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012326:RNA Splicing; D015183:Restriction Mapping; D016415:Sequence Alignment
D009838:Oligodeoxyribonucleotides; D000429:Alcohol Oxidoreductases; D050540:Ketol-Acid Reductoisomerase