Inactivation of simian rotavirus SA 11 by hydrogen peroxide.
The kinetics and extent of the simian rotavirus SA 11 (RV) inactivation by low concentrations of hydrogen peroxide (HP) were determined. The curves illustrating the kinetics of crude virus inactivation have "tailing off". The inactivation of the purified virus runs according to the first-order kinetics. Data indicated, that the purified RV was more sensitive to HP comparing to the crude virus. The purified RV was relatively sensitive to 0.5-1.0% HP. The nonpurified RV was considerably more resistant. Only HP concentrations of 3% and higher were efficient. Virus sensitivity to the disinfectant strongly depends on pH of a medium in which it is suspended. The lowest inactivation rate occurs at pH 7.4, the highest at pH 3.0. The strong temperature dependence of the virus inactivation rate by HP has been noticed. However no changes of the virus capsid after heating at 40 degrees C able to facilitate the HP action at 0 degrees C were observed. The HP action on virus particles at low temperatures does not make them more thermolabile, when the temperature shifts from 0 degrees C to 40 degrees C. So, the temperature dependence of inactivation rate is probably determined only by the reaction rate between a disinfectant and virus particles as targets. The horse-radish peroxidase in concentration 1-5-10 mg/ml considerably increased the virucidal effect of low HP concentrations. We think that mixtures of low HP concentrations with peroxidase are less disadvantageous than higher HP concentrations showing the same virucidal efficiency.
Institute for Preventive Toxicology and Disinfection, Russian Ministry of Health, Moscow.
E V
EV
Kiryanova
L G
LG
Panteleeva
G A
GA
Shirman
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004338:Drug Combinations; D006735:Horseradish Peroxidase / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D012401:Rotavirus / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature; D014771:Virion / Q000187:drug effects
D004338:Drug Combinations; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase