Preparation of peptide-carrying microspheres with bioactivity on platelets.
A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.
Kasuya
Y
Y
Faculty of Science and Technology, Keio University, Yokohama 223, Japan.
Fujimoto
K
K
Miyamoto
M
M
Juji
T
T
Otaka
A
A
Funakoshi
S
S
Fujii
N
N
Kawaguchi
H
H
D000178:Acrylamides; D000255:Adenosine Triphosphate / Q000097:blood; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D004337:Drug Carriers; D006801:Humans; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009842:Oligopeptides / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011137:Polystyrenes
D000178:Acrylamides; D001672:Biocompatible Materials; D004337:Drug Carriers; D007202:Indicators and Reagents; D009842:Oligopeptides; D010975:Platelet Aggregation Inhibitors; D011137:Polystyrenes; D000255:Adenosine Triphosphate; C046617:arginyl-glycyl-aspartyl-serine