Development of PCR method specific for Marek's disease virus.
A rapid polymerase chain reaction (PCR) assay specific for Marek's Disease Virus (MDV) was developed. This assay was able to detect MDV in inoculated chick kidney cells at dilutions of 10(-5). Negative PCR results were obtained using uninoculated chick cells, Marek's Disease Vaccine (SB), Herpesvirus of Turkeys (HVT) and Fowl Laryngotracheitis Vaccine (LT). Bursae, feathers and kidneys from MDV infected chickens were positive in the PCR assay. The same tissues from normal chickens were negative. This method required only 0.5 h for sample preparatory, 3 h for PCR application and 1 h for electrophoresis. Internal probe hybridization confirmed that the PCR products are from MDV, but this hybridization will not be necessary for future MDV detection.
Department of Food Science, University of Arkansas, Fayetteville, 72701.
W
W
Rong-Fu
J N
JN
Beasley
W W
WW
Cao
M F
MF
Slavik
M G
MG
Johnson
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens / Q000382:microbiology; D006564:Herpesviridae / Q000235:genetics; D001352:Herpesvirus 1, Gallid / Q000235:genetics; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D007668:Kidney / Q000382:microbiology; D008380:Marek Disease / Q000175:diagnosis* / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D013997:Time Factors