Agglutination of Streptococcus mutans serotype C cells but inhibition of Porphyromonas gingivalis autoaggregation by human lactoferrin.
The ability of various forms of human lactoferrin (LF) to agglutinate oral Streptococcus mutans, Strep. sobrinus, Strep. rattus, Strep. sanguis, Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans cells was studied spectrophotometrically. Fe3+ saturated LF was unable to agglutinate these bacteria, whereas iron-free LF (apo LF) effectively agglutinated Strep. mutans cells but not the other bacteria. The efficiency and rate of agglutination of Strep. mutans were somewhat lower with apo LF than with human whole saliva. However, secretory IgA, phosphate and whole saliva almost totally abolished the apo LF-mediated agglutination of Strep. mutans, suggesting binding to the same target sites on bacterial cell surfaces, or to each other. The presence of exogenous iron (Fe2+, Fe3+), lactoperoxidase or serum albumin did not affect the agglutination by apo LF. Low Ca2+ (50-100 microns) slightly enhanced the agglutination by apo LF but higher concentrations (0.5-1.0 mM) totally blocked the apo LF-mediated agglutination of Strep. mutans. Both saliva and apo LF significantly delayed the rapid autoaggregation of P. gingivalis cells. Aggregation of P. gingivalis is considered a potential virulence factor and a protective mechanism against the host's cellular defences in the gingival crevice. These findings show a novel, strain-specific antibacterial mechanism for LF against Strep. mutans and P. gingivalis and adds a new compound to the group of agglutinating proteins in human saliva.
Soukka
T
T
Institute of Dentistry, University of Turku, Finland.
Tenovuo
J
J
Rundegren
J
J
D000371:Agglutination; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D000818:Animals; D001059:Apoproteins / Q000494:pharmacology / Q000502:physiology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000502:physiology; D007781:Lactoferrin / Q000494:pharmacology / Q000502:physiology*; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D012463:Saliva / Q000502:physiology*; D012471:Salivary Proteins and Peptides / Q000502:physiology; D013291:Streptococcus / Q000187:drug effects; D013295:Streptococcus mutans / Q000187:drug effects* / Q000502:physiology; D013298:Streptococcus sanguis / Q000187:drug effects; D016986:Streptococcus sobrinus / Q000187:drug effects
D001059:Apoproteins; D007071:Immunoglobulin A, Secretory; D012471:Salivary Proteins and Peptides; C039223:apolactoferrin; D007781:Lactoferrin