A monoclonal antibody recognizing a differentiation marker on rat gonocytes.
Monoclonal antibodies (MAb) were raised against a testicular membrane fraction from 18-day post coitum (p.c.) rat testes. One antibody, designated 4B6.3E10 (mu, kappa), was obtained which specifically reacted with gonocytes in the fetal testis. No significant cross-reactivity with other tissues from the 18-day p.c. embryo was found. MAb 4B6.3E10 was reactive with rat gonocytes from 17-day p.c. until the day of birth. Germ cells at later stages of testis development did not show any labelling. The epitope recognized by 4B6.3E10 is a carbohydrate as periodate treatment leads to a loss of reactivity of the antibody. By SDS-PAGE and Western blotting of proteins extracted from a testicular membrane fraction from 18-day p.c. testes, MAb 4B6.3E10 was found to recognize at least 3 protein moieties with apparent molecular weights in the ranges of 80-100, 120, 160-180 kDa (either under reducing- or non-reducing conditions). The results suggest that MAb 4B6.3E10 recognizes a specific differentiation marker for fetal rat gonocytes.
Department of Veterinary Anatomy and Cell Biology, Veterinary School, Utrecht University, The Netherlands.
F M
FM
van Dissel-Emiliani
P J
PJ
van Kooten
M
M
de Boer-Brouwer
D G
DG
de Rooij
J A
JA
van der Donk
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D006825:Hybridomas / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D010504:Periodic Acid / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013091:Spermatogenesis / Q000276:immunology; D013737:Testis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D010504:Periodic Acid; C009288:metaperiodate