17 beta-Hydroxysteroid dehydrogenase activity of ovary and hepatopancreas of freshwater prawn, Macrobrachium rosenbergii: relation to ovarian condition and estrogen treatment.
The activity of 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) was determined in the hepatopancreas and the ovary of freshwater prawn Macrobrachium rosenbergii, at different stages of life. A uniform level of 17 beta-HSD activity was observed in the hepatopancreas of immature, maturing, matured, and gravid prawns, whereas the activity of this enzyme was found to be higher in the ovary of the maturing prawn only. Gradual increases in the GSI values and ovarian protein content at the different stages of maturity were observed in this animal. Between the two organs, higher activity of 17 beta-HSD was observed in the hepatopancreas of the prawn in the maturing stage. The estradiol-17 beta (E2) level measured by radioimmunoassay showed its peak in both the hemolymph (126.0 +/- 7.0 pg/ml) and the ovary (300.00 +/- 9.0 pg/mg protein) in the maturing prawn only compared to that in immature, matured, and gravid prawns. The hormone level became undetectable in the hemolymph and remained about 10-fold less (30.6 +/- 5.0 pg/mg protein) in the ovary of the gravid prawn in comparison to the prawns in the maturing stage. A parallelism between the nature of 17 beta-HSD activity and the hormone level was observed in the ovary at different stages of life. A dose-dependent stimulation of 17 beta-HSD activity was observed in the hepatopancreas of the maturing prawn after three consecutive injections of 0.25, 0.5, 1.0, and 2.0 micrograms/g of estradiol-17 beta (E2) on the 4th day of treatment compared to the control values.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Animal Physiology, Bose Institute, Calcutta, India.
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D
Ghosh
A K
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Ray
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004958:Estradiol / Q000097:blood / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006458:Hemolymph / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000254:growth & development; D010053:Ovary / Q000201:enzymology / Q000254:growth & development / Q000502:physiology; D033541:Palaemonidae / Q000201:enzymology / Q000502:physiology*; D010179:Pancreas / Q000201:enzymology / Q000254:growth & development; D013629:Tamoxifen / Q000494:pharmacology
D004967:Estrogens; D013629:Tamoxifen; D004958:Estradiol; D003513:Cycloheximide; D015067:17-Hydroxysteroid Dehydrogenases