Detection of low level of human papilloma virus type 16 DNA sequences in cancer cell lines derived from two well-differentiated nasopharyngeal cancers.
Human papilloma virus type 16 (HPV16) related sequences were detected in two EBV-negative nasopharyngeal carcinoma (NPC) cell lines derived from two well-differentiated NPC by polymerase chain reaction. E2 and E6 related sequences of HPV16 were demonstrated using two pairs of primers derived from these two regions. DNA sequence analysis of amplified products excluded the possibility of laboratory viral DNA contamination.
Institute of Biomedical Sciences, Academia Sinica, Taipei.
E S
ES
Huang
D
D
Gutsch
K W
KW
Tzung
C T
CT
Lin
D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D002454:Cell Differentiation; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology* / Q000473:pathology; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured / Q000382:microbiology / Q000473:pathology
D000956:Antigens, Viral; D015344:DNA Probes, HPV; D004279:DNA, Viral