[A comparative study of the diagnostic value of peptides, corresponding to various genomic sequences of the hepatitis delta virus].
A set of 5 peptides from the immunodominant region (65-80 aa) of delta-antigen was prepared by solid phase synthesis. Peptide 71-80 was synthesized in two variants with different amino acid residues in positions 73, 74, 76. Free peptides and their conjugates with bovine serum albumin were tested for antigenicity in ELISA. Correlation between the length of the peptide chain and its antigenic activity was noted. Peptides 65-80 and 69-80 demonstrated positive reaction with all sera from acute HDV patients. Both variants of peptide 71-80 reacted with 83% of positive sera. The smaller peptide 73-80 reacted strongly with one positive serum out of five used.
Semiletov
Iu A
IuA
Kruglov
I V
IV
Karpova
V A
VA
Iashina
T L
TL
Favorov
M O
MO
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005814:Genes, Viral; D003699:Hepatitis D / Q000175:diagnosis*; D003698:Hepatitis Delta Virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides
D000956:Antigens, Viral; D016056:Immunodominant Epitopes; D010455:Peptides