Single-seed PCR of LMW glutenin genes to distinguish between durum wheat cultivars with good and poor technological properties.
Polymerase chain reaction (PCR) was used to amplify low-molecular-weight (LMW) glutenin sequences from genomic DNA extracted from a single germinating seed of several durum wheat genotypes. Electrophoretic analysis of PCR reactions showed the presence of amplified products characteristic of durum wheat cultivars with good and poor technological properties. This PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting durum wheat genotypes with good qualitative characteristics.
Dipartimento di Agrobiologia e Agrochimica, Università degli Studi della Tuscia, Viterbo, Italy.
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D001483:Base Sequence; D017931:DNA Primers; D005838:Genotype; D005983:Glutens / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000737:chemistry; D014908:Triticum / Q000145:classification / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D005983:Glutens; C037302:glutenin