Bombesin receptor antagonists differentiate receptor subtypes in rat brain.
Previous studies have shown that various bombesin receptor antagonists can distinguish between bombesin receptor subtypes in peripheral tissues. To determine whether these antagonists would be useful in differentiating bombesin receptor subtypes in the rat central nervous system, we used in vitro receptor autoradiography to examine the binding affinities of several bombesin receptor antagonists for two brain regions we had characterized as possessing distinct bombesin receptor subtypes. Our results demonstrate that, consistent with peripheral bombesin receptors, bombesin receptor subtypes in the rat brain can be differentiated by various bombesin receptor antagonists.
Johns Hopkins University School of Medicine, Department of Psychiatry and Behavioral Sciences, Baltimore, MD 21205.
E E
EE
Ladenheim
R T
RT
Jensen
S A
SA
Mantey
J E
JE
Taylor
D H
DH
Coy
T H
TH
Moran
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D001839:Bombesin / Q000031:analogs & derivatives / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D019886:Gastrin-Releasing Peptide; D066298:In Vitro Techniques; D008297:Male; D015287:Neurokinin B / Q000031:analogs & derivatives* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter / Q000037:antagonists & inhibitors* / Q000378:metabolism
D010455:Peptides; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter; D019886:Gastrin-Releasing Peptide; D015287:Neurokinin B; C038393:neuromedin B; D001839:Bombesin