Analysis of hapten-carrier protein conjugates by nondenaturing gel electrophoresis.
Hapten-carrier protein conjugates were made using five different small haptens (MW < 1000), two carrier proteins and two methods of conjugation. Nondenaturing agarose gel electrophoresis was used to demonstrate that when as few as two molecules of a small hapten are attached to the carrier, the conjugate band migrates differently from that of the carrier alone or of the coupling reagent-treated carrier. Furthermore, the direction of the change in migration of each conjugate correlates with the change in charge which occurs upon attachment of the hapten to the carrier.
Kamps-Holtzapple
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Food Animal Protection Research Laboratory, U.S. Department of Agriculture, College Station, TX 77845.
Carlin
R J
RJ
Sheffield
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C
Kubena
L
L
Stanker
L
L
DeLoach
J R
JR
D002934:Cinnamates; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005029:Ethylenediamines / Q000737:chemistry; D037341:Fumonisins; D005665:Furosemide / Q000737:chemistry; D006241:Haptens / Q000032:analysis*; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000737:chemistry; D009183:Mycotoxins / Q000737:chemistry; D010047:Ovalbumin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D015025:Zearalenone / Q000031:analogs & derivatives / Q000737:chemistry
D002934:Cinnamates; D005029:Ethylenediamines; D037341:Fumonisins; D006241:Haptens; D009183:Mycotoxins; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D006921:Hygromycin B; C026273:hygromycin A; C056933:fumonisin B1; D015025:Zearalenone; C031234:ethylenediamine; D005665:Furosemide; D010047:Ovalbumin