Mutations in the yeast fructose 1,6-biphosphatase structural gene affect expression of a fructose 1,6-biphosphatase-endoglucanase A hybrid protein.
Mutations in the yeast fructose 1,6 biphosphatase structural gene severely reduced expression of a fructose 1,6 biphosphatase-endoglucanase A hybrid protein introduced into yeast on multicopy or centromeric vectors. Upon glucose limitation the mutant yeasts were incapable of de-repressing endoglucanase A synthesis.
Facultad de Biologia, Universidad de La Habana, Cuba.
A
A
Silva
J
J
Benitez
D001483:Base Sequence; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D003013:Clostridium / Q000201:enzymology; D004271:DNA, Fungal; D006597:Fructose-Bisphosphatase / Q000096:biosynthesis / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
D004271:DNA, Fungal; D011993:Recombinant Fusion Proteins; D006597:Fructose-Bisphosphatase; D002480:Cellulase