Immunohistochemical study of mucin carbohydrates and core proteins in hepatolithiasis and cholangiocarcinoma.
The expression of mucin carbohydrates [Tn, sialosyl-Tn(STn), and T antigens] and core proteins [MUCI-apomucin-related antigen (ARA) and MUC2-ARA] was examined immunohistochemically in tissues from 40 patients with hepatolithiasis and 26 patients with intrahepatic bile-duct carcinoma. Tn and STn antigens were expressed in most of the carcinomas, and were also often expressed in the atypical bile-duct epithelium of the patients with hepatolithiasis or carcinoma, whereas they were rarely or never expressed in the normal bile duct, suggesting that they are effective tumor markers. T antigen was less useful as a marker for intrahepatic bile-duct carcinoma or the atypical epithelium, because it was expressed in normal bile-duct of some cases. Regarding the expression of ARAs in the carcinomas, non-invasive bile-duct cyst adenocarcinomas with favorable prognosis either expressed no MUCI-ARA with [DF3(-), MUSEII(-) and 139H2(-)] staining pattern or expressed MUCI-ARA with [DF3(-), MUSEII(+) and 139H2(+)] staining pattern. However these tumors often expressed MUC2-ARA with [anti-MRP(+) and CCP58(+)] staining pattern. In contrast, most invasive non-papillary cholangiocarcinomas with poor prognosis expressed MUCI-ARA with [DF3(+), MUSEII(+) and 139H2(+)] staining pattern, but expressed no MUC2-ARA with [anti-MRP(-) and CCP58(-)] staining pattern. These results suggests that different apomucins are produced by bile-duct cystadenocarcinomas and cholangiocarcinomas with differing prognosis. Furthermore, expression of Tn and STn antigens is a useful indicator of malignancy in the intrahepatic duct.
Department of Pathology, Kagoshima University School of Medicine, Japan.
K
K
Yamashita
S
S
Yonezawa
S
S
Tanaka
H
H
Shirahama
K
K
Sakoda
K
K
Imai
P X
PX
Xing
I F
IF
McKenzie
J
J
Hilkens
Y S
YS
Kim
D002759:Adenoma, Bile Duct / Q000737:chemistry*; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D001650:Bile Duct Neoplasms / Q000737:chemistry*; D001653:Bile Ducts, Intrahepatic / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis; D002769:Cholelithiasis / Q000737:chemistry*; D003536:Cystadenocarcinoma / Q000737:chemistry; D005260:Female; D005752:Gastric Mucins; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D008969:Molecular Sequence Data; D018396:Mucin-1; D055262:Mucin-2; D009077:Mucins / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis
D015295:Antigens, Tumor-Associated, Carbohydrate; D002241:Carbohydrates; D005752:Gastric Mucins; C524625:MUC2 protein, human; D008562:Membrane Glycoproteins; D018396:Mucin-1; D055262:Mucin-2; D009077:Mucins; D009363:Neoplasm Proteins; D010455:Peptides; C053366:apomucin