Effects of cytokines on HLA class I antigen expression on Huh6 and HB611 cells.
This study investigated the expression of HLA class I antigens on Huh6 and HB611 cells induced by interferon (IFN)-alpha, IFN-gamma, tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta. All of these cytokines induced the antigens on both cells in a dose-dependent manner, with IFNs inducing much more expression than TNF-alpha or IL-1 beta. We have already reported that protein kinase C (PKC) is involved in the antigen expression induced by IFN-gamma on Huh6 cells. The antigen expression induced by IFN-alpha was also blocked by a PKC inhibitor, H-7. However, the antigen expression by TNF-alpha or IL-1 beta was not inhibited by H-7, by a protein kinase A inhibitor, HA1004, nor by a calmodulin antagonist, W-7. These results suggested that PKC, Ca(2+)-calmodulin, and cAMP are not involved in the induction of HLA class I antigens on both cells by TNF-alpha and IL-1 beta. We concluded that TNF-alpha and IL-1 beta induced much less expression of HLA class I antigens on both cells than IFNs and that this might be because the signaling pathway by TNF-alpha and IL-1 beta differed from that by IFNs.
Towata
T
T
First Department of Medicine, Osaka University Medical School, Japan.
Hayashi
N
N
Katayama
K
K
Takehara
T
T
Miyoshi
E
E
Kawanishi
Y
Y
Ueda
K
K
Kasahara
A
A
Fusamoto
H
H
Kamada
T
T
D006528:Carcinoma, Hepatocellular / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006515:Hepatitis B virus / Q000502:physiology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000276:immunology; D011493:Protein Kinase C / Q000502:physiology; D011994:Recombinant Proteins; D015398:Signal Transduction / Q000502:physiology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014779:Virus Replication / Q000502:physiology
D015395:Histocompatibility Antigens Class I; D007370:Interferon Type I; D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D011493:Protein Kinase C