Sequence of a Leishmania major gene encoding an ubiquitin fusion protein.
A gene encoding an ubiquitin-tail protein fusion was isolated from the parasitic protozoan, Leishmania major, and sequenced. The L. major tail protein shares 97, 96, 67, 62, 62 and 61% sequence identity with the tail proteins of Trypanosoma brucei, Trypanosoma cruzi, yeast, Dictyostelium discoideum, human, and Arabidopsis thaliana, respectively. The putative 'zinc finger' nucleic acid-binding domain found in all ubiquitin 'tail' or 'extension' proteins described is also conserved in the L. major sequence. The upstream sequence indicated that this gene is not located at the end of a polyubiquitin sequence.
Department of Molecular Biology, University of Wyoming, Laramie 82071-3944.
G R
GR
Graeff
P M
PM
Steele
C L
CL
Peterson
M L
ML
Bennett
P J
PJ
Langer
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D016384:Consensus Sequence; D017124:Conserved Sequence; D016054:DNA, Protozoan / Q000032:analysis; D004023:Dictyostelium / Q000235:genetics; D017125:Genes, Protozoan; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007895:Leishmania tropica / Q000235:genetics*; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D013045:Species Specificity; D014346:Trypanosoma brucei brucei / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics; D014452:Ubiquitins / Q000737:chemistry / Q000235:genetics*; D016335:Zinc Fingers
D016054:DNA, Protozoan; D006360:Heat-Shock Proteins; D011498:Protein Precursors; D014452:Ubiquitins; C045677:ubiquitin precursor