Metabolic characteristics of pig colonocytes after adaptation to a high fiber diet.
The capacities of viable colonic epithelial cells to metabolize glucose, glutamine and n-butyrate were studied in 30-kg pigs adapted to a high fiber (12% sugar beet fiber) or a low fiber diet. Glucose and glutamine were extensively utilized but predominantly not oxidized, whereas n-butyrate oxidation accounted for 45% of n-butyrate metabolism and was not greatly affected by the presence of glucose or glutamine. With both diets, glycolysis was the major pathway accounting for glucose disappearance. There was a sparing effect of n-butyrate on both glycolysis and glucose oxidation. Moreover, the glycolytic capacity was 25% lower in pigs fed the high fiber diet. Data suggest that 6-phosphofructo-1-kinase could be the regulatory step in glycolysis. Nevertheless, its maximum activity was not affected by the diet or by the presence of n-butyrate. Glutamine metabolism was slightly affected by fiber in the diet and by the presence of n-butyrate. In addition to CO2, butyrate was converted into ketone bodies. Glucose and glutamine did not substantially alter n-butyrate metabolism. We conclude that some metabolic features of pig colonocytes, such as the capacity to oxidize n-butyrate, resemble those of rat and human colonocytes. Moreover, some characteristics, such as the glycolytic capacity, can be modulated by the level of fiber in the diet.
Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy-en-Josas, France.
B
B
Darcy-Vrillon
M T
MT
Morel
C
C
Cherbuy
F
F
Bernard
L
L
Posho
F
F
Blachier
J C
JC
Meslin
P H
PH
Duee
D020155:3-Hydroxybutyric Acid; D000818:Animals; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D003106:Colon / Q000378:metabolism*; D004043:Dietary Fiber / Q000008:administration & dosage*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D010084:Oxidation-Reduction; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013552:Swine
D002087:Butyrates; D004043:Dietary Fiber; D006885:Hydroxybutyrates; D007657:Ketone Bodies; D007773:Lactates; D005973:Glutamine; D020148:Butyric Acid; D019344:Lactic Acid; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D005947:Glucose; D020155:3-Hydroxybutyric Acid