Intratumoral administration of neocarzinostatin conjugated to monoclonal antibody A7 in a model of pancreatic cancer.
We investigated the following in athymic nude mice with xenografts of a human pancreatic carcinoma: 1) clearance of the murine monoclonal antibody A7 from the carcinoma; and 2) the antitumor effect of neocarzinostatin conjugated to MAb A7 (A7-NCS) on the carcinoma following intratumoral injection. Compared with 125I-labeled normal mouse IgG, a significantly larger amount of 125I-labeled A7 remained in the tumor after intratumoral injection. Neocarzinostatin conjugated to MAb A7 showed a greater antitumor activity against human pancreatic cancer than neocarzinostatin alone after intratumoral administration. The conjugate completely suppressed tumor growth macroscopically during the experiment. Tumor tissue in mice became necrotic 32 days after injection with A7-NCS. These observations suggest that the intratumoral injection of A7-NCS offers promise in treating pancreatic carcinoma.
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
E
E
Otsuji
T
T
Yamaguchi
N
N
Yamaoka
K
K
Kitamura
N
N
Yamaguchi
T
T
Takahashi
D000818:Animals; D000911:Antibodies, Monoclonal; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000188:drug therapy*; D004337:Drug Carriers; D006801:Humans; D007124:Immunoenzyme Techniques; D015552:Injections, Intralesional; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D014407:Tumor Cells, Cultured; D009353:Zinostatin / Q000008:administration & dosage* / Q000493:pharmacokinetics
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D007457:Iodine Radioisotopes; D009353:Zinostatin