Variable TGF-beta receptor expression regulates TGF-beta responses of articular chondrocytes.
TGF-beta inhibits the proteoglycan synthesis of freshly isolated articular chondrocytes while TGF-beta stimulates the proteoglycan synthesis of articular chondrocytes cultured for 14 days. To investigate if this differential effect was the result of differences in TGF-beta receptor expression this parameter was studied by affinity labeling in combination with SDS-PAGE. Using this method we demonstrated a difference in size of the TGF-beta type II receptor between freshly isolated and cultured chondrocytes. This difference might explain the differential effect of TGF-beta on the proteoglycan synthesis of chondrocytes cultured for 1 or 14 days.
Glansbeek
H L
HL
Department of Rheumatology, University Hospital Nijmegen, The Netherlands.
van der Kraan
P M
PM
Vitters
E L
EL
van den Berg
W B
WB
D000818:Animals; D001345:Autoradiography; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D011509:Proteoglycans / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000494:pharmacology
D011509:Proteoglycans; D011956:Receptors, Cell Surface; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta