In vitro sensitization for human monoclonal antibody production.
We have found that cell adhesion prior to in vitro antigenic stimulation enriched the B-cell population and diminished the CD8 lymphocyte subset. These changes in lymphocyte proportions were favourable for increasing the percentage of antibody-producing cells after culturing in vitro followed by Epstein-Barr virus (EBV) infection. The immunodepletion of leukocytes by methyl esters did not yield satisfying results under analogous culture conditions. In vitro primary antigenic stimulations with the addition of IL-2 (25 U/ml medium) and low amounts of interferon-gamma provided better recruitment of antibody-producing cells and higher binding activity of antibodies to sperm than secondary antigenic stimulation. IL-6 did not positively influence the EBV-transformed cell lines.
Niedbała
W
W
Institute of Human Genetics, Polish Academy of Sciences, Poznan.
Kurpisz
M
M
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes; D002448:Cell Adhesion; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007158:Immunologic Techniques; D007371:Interferon-gamma; D007376:Interleukin-2; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D013094:Spermatozoa / Q000276:immunology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D007371:Interferon-gamma