Sequence analysis of cystine-containing peptides in the Na+, K(+)-ATPase alpha subunit.
Tryptic digest of the pig kidney Na+, K(+)-ATPase was subjected to HPLC for separating water-soluble fragments. Using ammetric titration with silver nitrate, disulfide-containing peptides were identified and demonstrated to contain s-s bonds with differential sensitivity to reduction. The amino acid sequences of cystine-containing peptides were determined by chemical modification of cysteine residues during the sequence analysis. Among the cystine-containing peptides, three fragments of the alpha-subunit polypeptide chain were identified: Cys452-Lys461, Ile507-Lys519, Val545-Phe558.
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
N M
NM
Gevondyan
V S
VS
Gevondyan
N N
NN
Modyanov
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003553:Cystine / Q000032:analysis*; D004220:Disulfides / Q000032:analysis; D007679:Kidney Medulla / Q000201:enzymology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry*; D013552:Swine; D014357:Trypsin
D004220:Disulfides; D046911:Macromolecular Substances; D010446:Peptide Fragments; D003553:Cystine; D014357:Trypsin; D000254:Sodium-Potassium-Exchanging ATPase