Localization of inhibin/activin subunit mRNAs during the luteal phase in the primate ovary.
Localization of inhibin/activin subunit mRNAs within the macaque ovary from the immediate pre-ovulatory period of the menstrual cycle, when serum immunoreactive inhibin begins to rise, to day 9 of the luteal phase, when serum inhibin concentrations are maximal, was investigated using in-situ hybridization. Ovaries were studied on the day of the LH surge (day 0) and on days 2, 5, and 9 of the luteal phase by hybridizing frozen tissue sections with radiolabelled riboprobes specific to the inhibin/activin alpha-, beta A- and beta B-subunits. After autoradiographic exposure for 10 and 21 days, grain concentrations were quantified by image analysis. Moderate expression of alpha-, beta A- and beta B-subunit mRNA was present within the granulosa cells of the pre-ovulatory follicle (day 0). The granulosa-lutein cells of the corpora lutea expressed high levels of alpha-subunit at days 2, 5 and 9. mRNAs for beta A and beta B were detected at low but significant levels in all of the corpora lutea. All healthy antral follicles exhibited a high level of expression of beta B-subunit mRNA in the granulosa cells. On day 2 after ovulation these follicles also expressed high alpha- and moderate beta A-subunit mRNA. On day 9 the beta B-inhibin mRNA in antral follicles was found in association with low expression of the other subunits. Small follicles in ovaries on day 2 expressed moderate alpha- and low levels of beta B-subunit mRNA, while mRNA for beta A was absent. alpha-subunit mRNA expression was present on day 5 while neither beta A- nor beta B-subunit mRNA was detected. On day 9 a proportion of small follicles expressed alpha- and beta A-subunit mRNA. These results demonstrate that marked differences are present in the levels of expression of the three inhibin/activin subunit genes between follicles and the corpus luteum. The predominance of the beta B-subunit mRNA within antral follicles would be consistent with the synthesis of activin. The predominance of the alpha-subunit combined with the low expression of the beta-subunits in the corpus luteum suggests that both biologically active inhibin and free alpha-subunit are produced by the primate corpus luteum.
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK.
H M
HM
Fraser
S F
SF
Lunn
G M
GM
Cowen
P T
PT
Saunders
D028341:Activins; D000818:Animals; D005260:Female; D017403:In Situ Hybridization; D007265:Inhibins / Q000235:genetics / Q000378:metabolism*; D008183:Luteal Phase / Q000378:metabolism*; D008251:Macaca; D010053:Ovary / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D012333:RNA, Messenger; D028341:Activins; D007265:Inhibins