RRR-alpha-tocopheryl succinate induced interleukin-2 production by avian splenic T lymphocytes and murine EL-4 thymic lymphoma cells.
RRR-alpha-tocopheryl succinate (vitamin E succinate) was studied for its effects on interleukin-2 (IL-2) production by chicken splenic derived T lymphocytes and murine EL-4 thymic lymphoma cells. Supernatants from 0.1 microgram/mL vitamin E succinate-supplemented chicken splenic T cell cultures exhibited 42-72% enhanced IL-2 production over vehicle controls when tested in a chicken T cell blast bioassay. Supplementation of chicken splenic T lymphocyte cultures with butylated hydroxyanisole (BHT) and butylated hydroxytoluene (BHA) also induced elevated levels of IL-2, suggesting a role for antioxidants in IL-2 production by avian splenic T lymphocytes. Supernatants from vitamin E succinate-supplemented murine EL-4 cells (0.1 microgram/mL vitamin E succinate) induced 52-75% increased levels of IL-2 when compared to supernatants from vehicle controls when tested using a murine, IL-2-dependent CTLL-2 bioassay. IL-2 production by EL-4 cells was not enhanced by treatments with BHT, BHA, or Trolox, suggesting that vitamin E succinate-induced IL-2 production by EL-4 cells may involve a mechanism other than antioxidant effects. Vitamin E succinate plus suboptimal levels of the protein kinase C (PKC) activator phorbol myristate acetate (PMA) induced the highest levels of IL-2 by EL-4 cells. The studies provide evidence that vitamin E succinate can directly potentiate either the production or release of IL-2 from avian splenocytes and murine EL-4 cells.
University of Texas, Austin.
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Kidao
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Sanders
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D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002478:Cells, Cultured; D002645:Chickens; D002839:Chromans / Q000494:pharmacology; D004357:Drug Synergism; D007376:Interleukin-2 / Q000096:biosynthesis*; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology; D013953:Thymus Neoplasms / Q000378:metabolism*; D024505:Tocopherols; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology
D000975:Antioxidants; D002839:Chromans; D007376:Interleukin-2; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D013755:Tetradecanoylphorbol Acetate; D024505:Tocopherols; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid