Effect of fluoride on the ATP requirement for glycerolipid biosynthesis in adipose tissue of four mammalian species.
1. Maximal glycerolipid biosynthesis activity was determined with 700 g homogenates of human, ovine, rat and bovine adipose tissue, and in the presence of varied concentrations of ATP and fluoride (as KF). 2. Minimal responses to either ATP or KF were observed with human homogenates. 3. Increasing ATP, from 4 to 20 mM, markedly increased glycerolipid biosynthesis in ovine and rat homogenates, but not in those of the bovine; similar responses were observed with 4 mM ATP plus 12.5 mM KF for rat and ovine. 4. Results indicated that KF inhibited ATP degradation in adipose tissue homogenates and that the severity of ATP degradation differed with species.
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Department of Animal Science, University of Wyoming, Laramie 82071.
D000255:Adenosine Triphosphate / Q000502:physiology*; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D005459:Fluorides / Q000494:pharmacology*; D006801:Humans; D008055:Lipids / Q000096:biosynthesis*; D008322:Mammals / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors*; D051381:Rats; D012756:Sheep
D008055:Lipids; D000255:Adenosine Triphosphate; D010744:Phosphoric Monoester Hydrolases; D005459:Fluorides