Characterization of clonality of Epstein-Barr virus-induced human B lymphoproliferative disease in mice with severe combined immunodeficiency.
To improve the diagnostic accuracy and understanding of the pathogenesis of lymphoproliferative diseases (LPDs) occurring in immunosuppressed transplant recipients (post-transplantation LPD), clonality of Epstein-Barr virus-induced human LPDs in mice with severe combined immunodeficiency was examined by analyzing: 1) human immunoglobulin genes and their products, 2) the clonality of Epstein-Barr virus DNA, and 3) genetic alteration of c-myc or bcl-2 genes. A spectrum of clonality was found in the LPDs comparable with that reported for post-transplantation LPDs, although rearrangements of c-myc or bcl-2 genes were not detected. It is confirmed that this system is useful in terms of clonality for understanding the early phases in the pathogenesis of post-transplantation LPD or LPD in immune deficient patients.
Nakamine
H
H
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-3135.
Masih
A S
AS
Okano
M
M
Taguchi
Y
Y
Pirruccello
S J
SJ
Davis
J R
JR
Mahloch
M L
ML
Beisel
K W
KW
Kleveland
K
K
Sanger
W G
WG
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D002472:Cell Transformation, Viral; D005803:Genes, Immunoglobulin; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D016130:Immunophenotyping; D008232:Lymphoproliferative Disorders / Q000209:etiology / Q000235:genetics / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D016511:Severe Combined Immunodeficiency / Q000209:etiology / Q000235:genetics* / Q000276:immunology*