High-performance liquid chromatographic determination of diclofenac in human plasma using automated column switching.
A validated high-performance liquid chromatographic procedure employing ultraviolet detection for the analysis of diclofenac in human plasma is reported. The method is rapid and, coupled with column switching, leads to a sensitive, accurate and reproducible assay. The retention times of diclofenac and the internal standard (4'-methoxydiclofenac, CGP-4287) are 6.4 and 7.6 min, respectively. The peak height versus plasma concentration is linear over the range 5.0-2000 ng/ml with a detection limit below 2.5 ng/ml. The mean absolute recovery of diclofenac using the described assay is 96.5% (n = 24). The inter- and intra-day accuracy and precision are within 8.3% of the actual values for all concentrations investigated. Furthermore, this procedure is applied to assess the pharmacokinetics of a single 75-mg oral dose of diclofenac sodium.
Analytical Research Center, L.A.B. Bio-Research International Inc., Senneville, Montreal, Quebec, Canada.
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Miller
D002851:Chromatography, High Pressure Liquid; D004008:Diclofenac / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D012015:Reference Standards; D013048:Specimen Handling; D013056:Spectrophotometry, Ultraviolet
D004008:Diclofenac