Diagnostic compatibility of a thymidine kinase, inverted repeat, gI, and gpX modified live gene-deleted PRV vaccine with three differential ELISAs.
The differential pseudorabies virus (PRV) vaccines currently in use in the USA have deletions of the genes coding for the glycoprotein I (gI) and/or glycoprotein X (gpX). The absence of gI and/or gpX allow for the serologic differentiation of vaccinated swine from PRV-infected swine using differential enzyme-linked immunosorbent assays (ELISAs). A newly developed pseudorabies vaccine virus has 4 deletions of the viral genome: the genes coding for gI, gpX, and thymidine kinase and a portion of the repeat region to attenuate the virus. The purpose of this work was to evaluate the diagnostic compatibility of the gI/gpX gene-deleted vaccine with 3 differential vaccines and 3 differential ELISAs currently in use. Pigs vaccinated 3 times with the gI/gpX gene-deleted vaccine remained seronegative on the 3 differential ELISAs tested. Pigs previously vaccinated with either a gI or gpX gene-deleted vaccine and then vaccinated with the gI/gpX gene-deleted vaccine remained seronegative on the respective gI or gpX differential assay.
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011.
S L
SL
Swenson
J
J
McMillen
H T
HT
Hill
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D007446:Chromosome Inversion; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D017353:Gene Deletion; D005814:Genes, Viral / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D011557:Pseudorabies / Q000175:diagnosis* / Q000276:immunology; D022421:Pseudorabies Vaccines; D012091:Repetitive Sequences, Nucleic Acid; D012698:Serologic Tests; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology; D013937:Thymidine Kinase / Q000235:genetics; D014613:Vaccines, Attenuated / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics
D000914:Antibodies, Viral; D022421:Pseudorabies Vaccines; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins; D013937:Thymidine Kinase