Caprine arthritis-encephalitis lentivirus SU is the ligand for infection of caprine synovial membrane cells.
Infection of goat synovial membrane (GSM) cells by caprine arthritis-encephalitis virus (CAEV) was inhibited by incubation of cells with CAEV gp 135 envelope glycoprotein (SU) expressed by recombinant vaccinia virus. Incubation of cells with protein expressed by a control recombinant vaccinia virus without the CAEV envelope gene did not inhibit CAEV infection. Removal of recombinant SU from blocking medium by adsorption with anti-SU IgG/protein G-Sepharose complexes resulted in loss of CAEV inhibition. Results support that CAEV infection of GSM cells is mediated by a specific interaction between SU and a cell surface receptor or receptor complex.
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164.
G A
GA
Hullinger
D P
DP
Knowles
T C
TC
McGuire
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WP
Cheevers
D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000378:metabolism; D016088:Arthritis-Encephalitis Virus, Caprine / Q000254:growth & development / Q000378:metabolism*; D001667:Binding, Competitive; D002478:Cells, Cultured; D015686:Gene Products, env / Q000737:chemistry / Q000378:metabolism*; D006041:Goats; D066298:In Vitro Techniques; D008024:Ligands; D008970:Molecular Weight; D011991:Receptors, Virus / Q000378:metabolism; D013583:Synovial Membrane / Q000382:microbiology; D014779:Virus Replication
D000956:Antigens, Viral; D015686:Gene Products, env; D008024:Ligands; D011991:Receptors, Virus