[The effect of silica dust on type I and type III collagen mRNA synthesis by fibroblasts in vitro].
Human alpha 1(I), alpha 2(I) and alpha 1(III) cDNA probes and RNA dot hybridization were employed to quantitate collagen mRNA changes after adding silica dust into the media of human 2BS fibroblasts. At all dosages used (100, 200, 500, and 1000 micrograms), the alpha 1(I), alpha 2(I) and alpha 1(III) mRNA levels increased one day after dusting. At the same dosage of silica (100 micrograms), alpha 1(III) mRNA increased more rapidly than did type I collagen mRNA. The type I and type III collagen mRNA contents in the experimental groups were higher than those in controls on days 3, 5, 7 and 9.
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing.
B
B
Liu
D002460:Cell Line; D003094:Collagen / Q000235:genetics*; D015342:DNA Probes; D004391:Dust; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000187:drug effects; D012822:Silicon Dioxide / Q000633:toxicity*
D015342:DNA Probes; D004391:Dust; D012333:RNA, Messenger; D012822:Silicon Dioxide; D003094:Collagen