Intramolecular electron transfer in ferredoxin II from Desulfovibrio desulfuricans Norway.
In order to elucidate the role of the two (4Fe-4S) clusters in ferredoxins and to determine whether an electron-transfer mechanism may occur between the clusters, the in vitro reduction of cytochrome c3 and cytochrome c553 by Desulfovibrio desulfuricans Norway ferredoxin II was studied using spectrophotometric techniques. Ferredoxin II, covalently cross-linked with either cytochrome c3 or c553, is an obligate intermediate in cytochrome reduction by pyruvate dehydrogenase. Both titration of the complex formation under 1H-NMR spectroscopy and cross-linking experiments between ferredoxin II and either cytochrome c3 or cytochrome c553 gave a stoichiometric ratio of 1:1. Modelling the protein yielded differences between the charge distributions around the two (Fe-S) clusters. The fact that Cluster 2 is blocked in the electron-transfer domain facing the cytochrome interacting heme, indicates Cluster 1 receives electron from pyruvate dehydrogenase. Consecutively, cytochrome reduction occurs owing to an intramolecular electron exchange between the two clusters of the ferredoxin. The properties of two (Fe-S) cluster ferredoxins are compared to those of monocluster ferredoxins and discussed in evolutionary terms.
Blanchard
L
L
Laboratoire de Chimie Bactérienne, CNRS, Marseille, France.
Payan
F
F
Qian
M
M
Haser
R
R
Noailly
M
M
Bruschi
M
M
Guerlesquin
F
F
D000595:Amino Acid Sequence; D003574:Cytochrome c Group / Q000737:chemistry; D003901:Desulfovibrio / Q000378:metabolism*; D004579:Electron Transport; D005288:Ferredoxins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry
D003574:Cytochrome c Group; D005288:Ferredoxins; D011768:Pyruvate Dehydrogenase Complex; C083951:cytochrome c553; C017165:cytochrome c(3); C022302:cytochrome C-552