Epithelial cell membrane specific, novel monoclonal antibody KA-1.
In order to develop a novel monoclonal antibody reacting with the cell membrane protein which could be a receptor for cell growth, differentiation or an adhesion molecule, keratinocytes from psoriatic epidermis and A-431 cell line were used to immunize BALB/c mice. A monoclonal antibody, KA-1 was developed. KA-1 reacts with the cell membranes of the whole epidermis with the exception of the stratum corneum cells and the basal cell membrane in contact with the basement membrane. In electron microscopy, the antigenic site was located on the surface of the microvilli of the cultured keratinocytes, especially at the contact area with another villi, the cell membrane or collagen. The antigenic protein was a 44-kDa protein which was different from E-cadherin in both molecular weight and cross-reactivity.
Department of Dermatology, Kinki University School of Medicine, Osaka, Japan.
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Harada
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Yamada
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Tezuka
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry* / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008871:Microvilli / Q000648:ultrastructure; D008970:Molecular Weight; D011565:Psoriasis / Q000473:pathology; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism / Q000502:physiology
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D011956:Receptors, Cell Surface