Identification of a G protein coupled receptor induced in activated T cells.
Many genes are induced after T cell activation to make a cell competent for proliferation and ultimately, function. Many of these genes encode surface receptors for growth factors that signal a cell to proliferate. We have cloned a novel gene (clone 6H1) that codes for a member of the G protein-coupled receptor superfamily. This gene was isolated from a chicken activated T cell cDNA library by low level hybridization to mammalian IL-2 cDNA probes. The 308 amino acid open reading frame has seven hydrophobic, presumably transmembrane domains and a consensus site for interaction with G proteins. Tissue distribution studies suggest that gene expression is restricted to activated T cells. The message appears by 1 h after activation and is maintained for at least 45 h. Transcription of 6H1 is induced by a number of T cell stimuli and is inhibited by cyclosporin A, but not by cycloheximide. This is the first description of a member of this superfamily expressed specifically in activated T cells. The gene product may provide a link between T cell growth factors and G protein activation.
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
M H
MH
Kaplan
D I
DI
Smith
R S
RS
Sundick
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000502:physiology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D043562:Receptors, G-Protein-Coupled; D018048:Receptors, Purinergic P2; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology; D014158:Transcription, Genetic; D049368:Receptors, Lysophosphatidic Acid
D019204:GTP-Binding Proteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D018048:Receptors, Purinergic P2; C082320:LPAR4 protein, human; D049368:Receptors, Lysophosphatidic Acid