NF-kappa B-binding proteins induced by HSV-1 infection of U937 cells are not involved in activation of human immunodeficiency virus.
Molecular interactions between the herpes simplex virus type 1 (HSV-1) and human immunodeficiency virus (HIV) were investigated in U937 cells. Nonpermissive HSV-1 infection of U937 cells activated HIV as determined in transient expression assays with hybrid constructions containing the HIV-LTR-directed chloramphenicol acetyltransferase gene. Activation was independent of kappa B-enhancer elements whereas these elements were required for HSV-1-mediated activation in another cell line (C6). kappa B-binding proteins were induced in U937 cells by HSV-1 infection. Four species (45, 55, 75, and 75/80 kDa) were identified by DNA-protein cross-linking. Methylation interference analysis defined close contact only with the third residue of the previously established critical contact triplet GGG. Transient expression assays using mutants in HIV-LTR revealed the presence a cis-response element (GGTCA palindrome) in the negative regulatory region.
Department of Pharmacology and Experimental Therapeutics, University of Maryland, School of Medicine, Baltimore 21201.
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Feng
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Kulka
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Aurelian
D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism*; D002472:Cell Transformation, Viral / Q000502:physiology*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D002999:Clone Cells; D015342:DNA Probes; D006678:HIV / Q000235:genetics* / Q000254:growth & development*; D016325:HIV Long Terminal Repeat; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000096:biosynthesis / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D018139:Simplexvirus / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014775:Virus Activation
D015342:DNA Probes; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase