Notes on the application of microwaves for antigen retrieval in paraffin and plastic tissue sections.
In formalin-fixed, paraffin-embedded tissue enhanced or de novo immunostaining can be obtained by microwave boiling of sections in a metal salt or buffer solution. In this paper this new technique is reviewed and important factors influencing final results are discussed. Microwave antigen retrieval can also be applied for immunohistochemistry on plastic GMA sections. Here the action of the microwave method is probably mainly due to breaking the bonds between GMA and proteins prohibiting immunostaining. The microwave methods do not require trypsin treatment.
Suurmeijer
A J
AJ
Pathology Laboratory, Martini Hospital, Groningen, The Netherlands.
Boon
M E
ME
D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D000941:Antigens / Q000032:analysis*; D016477:Artifacts; D015415:Biomarkers / Q000032:analysis; D002712:Chlorides; D002951:Citrates; D005188:False Negative Reactions; D006358:Hot Temperature; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007854:Lead; D008689:Methacrylates; D008872:Microwaves; D010232:Paraffin; D011506:Proteins / Q000032:analysis / Q000276:immunology; D013431:Sulfates; D013861:Thiocyanates; D016610:Tissue Embedding; D017967:Zinc Compounds; D019287:Zinc Sulfate
D017607:Aluminum Compounds; D000941:Antigens; D015415:Biomarkers; D002712:Chlorides; D002951:Citrates; D008689:Methacrylates; D011506:Proteins; D013431:Sulfates; D013861:Thiocyanates; D017967:Zinc Compounds; D007854:Lead; D000077410:Aluminum Chloride; C005044:hydroxyethyl methacrylate; D019287:Zinc Sulfate; D010232:Paraffin