Expression, identification and subcellular localization of the proteins encoded by the hepatitis C viral genome.
We have expressed the full-length coding region and selected domains of the hepatitis C virus (HCV) cDNA in mammalian cells by transfection. Using HCV antibody-positive human sera and monospecific antibodies the proteins encoded by the putative structural and non-structural regions of the open reading frame of HCV were identified as core (p22), E1 (gp32-35), E2 (gp68-72), NS2 (p23), NS3 (p72), NS4a and b (p10 and p27) and NS5a and b (p56 and p70). We have also defined the subcellular localizations of the HCV proteins using indirect immunofluorescence assays.
Chiron Corporation, Emeryville, California 94608.
M J
MJ
Selby
Q L
QL
Choo
K
K
Berger
G
G
Kuo
E
E
Glazer
M
M
Eckart
C
C
Lee
D
D
Chien
C
C
Kuo
M
M
Houghton
D000818:Animals; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D011994:Recombinant Proteins; D014764:Viral Proteins