Simian immunodeficiency virus-specific cytotoxic T lymphocytes in rhesus monkeys: characterization and vaccine induction.
An effective HIV vaccine should be capable of eliciting virus-specific cytotoxic T lymphocytes (CTL). We have characterized the cellular and molecular features of a simian immunodeficiency virus of macaques (SIVmac) gag-specific CTL response in rhesus monkeys. We have shown that SIVmac-infected rhesus monkeys expressing the major histocompatibility complex (MHC) class I molecule Mamu-A*01 develop a SIVmac gag-specific CTL response which recognizes a 9 amino acid fragment of the gag protein in association with Mamu-A*01. Moreover, this peptide/MHC class I recognition is mediated by T cell receptors (TCR) employing a predominant V beta gene family and J beta gene. Using this understanding of a SIVmac-specific CTL response, we have shown that SIVmac-specific CTL can be elicited through three novel approaches to vaccination: a recombinant viral vector, a recombinant bacterial vector and a peptide vaccine. These studies illustrate the utility of the SIV/macaque model in AIDS vaccine research.
Letvin
N L
NL
Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.
Miller
M D
MD
Shen
L
L
Chen
Z W
ZW
Yasutomi
Y
Y
D000818:Animals; D015686:Gene Products, env / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D008081:Liposomes; D008253:Macaca mulatta / Q000276:immunology*; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000473:pathology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000473:pathology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics; D014760:Viral Fusion Proteins; D014765:Viral Vaccines
D015686:Gene Products, env; D015395:Histocompatibility Antigens Class I; D008081:Liposomes; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D015682:Retroviridae Proteins, Oncogenic; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; D014765:Viral Vaccines; C059580:transmembrane protein, Simian immunodeficiency virus