Identification of a mobile endogenous transposon in Arabidopsis thaliana.
A mobile endogenous transposable element, Tag1, has been identified in the plant Arabidopsis thaliana. Tag1 was found in the nitrate transporter gene, CHL1, of a chlorate-resistant mutant present in a population of plants containing an active maize Ac transposon. Tag1 excises from the chl1 gene producing chlorate-sensitive revertants with Tag1 or Tag1-related elements at different loci. Tag1 and related elements are present in the Landsberg but not Columbia or Wassilewskija ecotypes of Arabidopsis. Thus, Tag1 provides a tool for the insertional mutagenesis of plant genes essential for biological processes of agronomic importance.
Tsay
Y F
YF
Department of Biology, University of California, San Diego, La Jolla 92093-0116.
Frank
M J
MJ
Page
T
T
Dean
C
C
Crawford
N M
NM
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002704:Chlorates / Q000494:pharmacology; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004251:DNA Transposable Elements; D004351:Drug Resistance; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D009566:Nitrates / Q000378:metabolism; D030821:Plants, Genetically Modified
D002704:Chlorates; D004251:DNA Transposable Elements; D009566:Nitrates; D004247:DNA