Significance of a newly identified attenuating mutation in Sabin 3 oral poliovirus vaccine.
Sequence analysis of poliovirus vaccine RNA has resulted in the identification of a new point mutation at position 2493 in Sabin 3. cDNA-derived Sabin 3 virus whose genome includes the new mutation has phenotypic properties expected for a vaccine strain. Virus engineered to contain a single base substitution at 2493 has lost the small plaque phenotype, and low neurovirulence characteristics normally associated with Sabin poliovirus strains. The significance of these observations and a potential relationship to the frequency of vaccine-associated poliomyelitis are described.
Mento
S J
SJ
Lederle-Praxis Biologicals, Pearl River, NY 10965.
Weeks-Levy
C
C
Tatem
J M
JM
Gorgacz
E J
EJ
Waterfield
W F
WF
D000818:Animals; D001482:Base Composition; D004247:DNA / Q000235:genetics; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D011051:Poliomyelitis / Q000453:epidemiology / Q000209:etiology / Q000473:pathology; D017955:Poliovirus / Q000235:genetics* / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000009:adverse effects / Q000633:toxicity; D012367:RNA, Viral / Q000235:genetics*; D014481:United States / Q000453:epidemiology; D014709:Vero Cells; D014774:Virulence / Q000235:genetics
D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral; D004247:DNA