Identification of baroreceptor reflex interneurons in the caudal ventrolateral medulla.
Interrupting neuronal activity in the caudal ventrolateral medulla (CVL) abolishes baroreceptor-mediated sympathoinhibition and vasodepressor responses. In the present study we identified CVL neurons that function as interneurons in the baroreceptor reflex arc. The mean antidromic onset latency of CVL neurons (5 ms) after stimulation in the rostral ventrolateral medulla (RVL) suggests that they transmit information to the RVL via unmyelinated axons. Stimulation of baroreceptor afferents in the aortic depressor nerve (ADN) excited CVL neurons projecting to the RVL with onset latencies between 15 and 45 ms. The short-latency ADN stimulus-evoked excitation of CVL barosensory interneurons preceded the onset of the ADN stimulus-evoked inhibition of RVL-spinal sympathoexcitatory neurons. Longer-latency ADN-evoked CVL neuronal responses may arise from activation of unmyelinated ADN afferents that can prolong the inhibition of RVL sympathoexcitatory neurons. The excitation of CVL barosensory interneurons resulting from the systolic pressure rise was followed by a period of decreased excitability of RVL sympatho-excitatory neurons, which was of comparable duration to the increase in the discharge probability of CVL neurons. Our characterization of baroreceptor reflex interneurons in the CVL suggests that transmission of baroreceptor information involves a complex activation of medullary interneurons responding selectively to different aspects of the arterial pressure stimulus.
Jeske
I
I
Department of Neurology and Neuroscience, Cornell University Medical College, New York, New York 10021.
Morrison
S F
SF
Cravo
S L
SL
Reis
D J
DJ
D000818:Animals; D001011:Aorta / Q000294:innervation; D001794:Blood Pressure / Q000502:physiology; D004558:Electric Stimulation; D006321:Heart / Q000502:physiology; D007395:Interneurons / Q000502:physiology*; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000502:physiology*; D009424:Nervous System Physiological Phenomena; D009433:Neural Inhibition / Q000502:physiology; D011311:Pressoreceptors / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology; D013153:Splanchnic Nerves / Q000166:cytology / Q000502:physiology; D013564:Sympathetic Nervous System / Q000166:cytology / Q000502:physiology; D009435:Synaptic Transmission