Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance.
We have used surface plasmon resonance biosensor technology to monitor the assembly and dynamics of a signal transduction complex which controls chemotaxis in Escherichia coli. A quaternary complex formed which consisted of the response regulator CheY, the histidine protein kinase CheA, a coupling protein CheW and a membrane-bound chemoreceptor Tar. Using various experimental conditions and mutant proteins, we have shown that the complex dissociates under conditions that favour phosphorylation of CheY. Direct physical analysis of interactions among proteins in this signal transduction pathway provides evidence for a previously unrecognized binding interaction between the kinase and its substrate. This interaction may be important for enhancing substrate specificity and preventing 'crosstalk' with other systems. The approach is generally applicable to furthering our understanding of how signalling complexes transduce intracellular messages.
Schuster
S C
SC
Division of Biology, California Institute of Technology, Pasadena 91125.
Swanson
R V
RV
Alex
L A
LA
Bourret
R B
RB
Simon
M I
MI
D001426:Bacterial Proteins / Q000378:metabolism*; D015374:Biosensing Techniques; D002628:Chemoreceptor Cells; D002633:Chemotaxis / Q000502:physiology*; D004800:Enzymes, Immobilized; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins; D000071677:Histidine Kinase; D007700:Kinetics; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000378:metabolism; D000072236:Methyl-Accepting Chemotaxis Proteins; D009154:Mutation; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface; D015398:Signal Transduction
D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D011956:Receptors, Cell Surface; C057331:Tar protein, E coli; C000607606:cheY protein, E coli; D011494:Protein Kinases; D000071677:Histidine Kinase; C000607608:cheA protein, E coli