A radioimmunoassay for human urinary prealbumin.
We describe a validated radioimmunoassay for prealbumin in urine. Using timed overnight urine samples, the normal reference range was less than 10-148 micrograms/L; or less than 1.8-9.6 micrograms/mmol creatinine, excretion in women being significantly greater than in men (P < 0.05); or less than 7.3-114 ng/min with no significant difference in excretion rate between the sexes. Urines exhibited loss of immunoreactivity after storage at -20 degrees C and thawing. No such loss occurred after storage for 4 weeks at 4 degrees C or room temperature. The urinary excretion of prealbumin was highly correlated with that of albumin (r = 0.85), and clearance relative to creatinine was 2 x 10(-6), the same order as that of albumin.
Department of Immunology, Westminster Hospital, London, UK.
R
R
Beetham
A
A
Dawnay
C
C
Ghany
S
S
Dubrey
J
J
Miles
D000293:Adolescent; D000328:Adult; D003404:Creatinine / Q000652:urine; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011228:Prealbumin / Q000652:urine*; D011863:Radioimmunoassay; D012016:Reference Values
D011228:Prealbumin; D003404:Creatinine