Electric stimulation regulates the level of Ca-channels in chick muscle culture.
To examine the role of muscle activity in the expression of 1,4-dihydropyridine (DHP)-sensitive Ca-channels, we electrically stimulated chick skeletal muscle cells growing in culture. The number of Ca-channels was determined by a radioligand binding technique using [3H]PN200-110. The function of these channels was measured by depolarization-induced 45Ca uptake. We found that both the amount and the function of these channels were higher as a result of the increased muscle activity caused by electrical stimulation (ES).
Otto Meyerhoff Drug Receptor Center, Department of Life Sciences, Bar-Ilan University, Ramat Gan Israel.
M
M
Freud-Silverberg
A
A
Shainberg
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002642:Chick Embryo; D046508:Culture Techniques; D004558:Electric Stimulation; D017275:Isradipine / Q000378:metabolism; D009132:Muscles / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D011188:Potassium / Q000494:pharmacology
D015220:Calcium Channels; D000110:Acetylcholinesterase; D011188:Potassium; D017275:Isradipine