In situ hybridization and northern blot demonstration of HLA-G mRNA in human trophoblast populations by locus-specific oligonucleotide.
Trophoblast cells from normal first trimester pregnancies have been shown to express the nonclassical class I molecule, HLA-G, which is nonpolymorphic and has a heavy chain of 40 kD. In this study, in situ hybridization experiments were performed using a biotinylated HLA-G specific oligonucleotide on sections of normal placenta including the implantation site. HLA-G mRNA was identified in all extravillous trophoblast populations including the cytotrophoblast cell columns, interstitial trophoblast, endovascular trophoblast, and placental bed giant cells. In addition, villous cytotrophoblast and villous mesenchymal cells also contained HLA-G transcripts, but villous syncytiotrophoblast was always negative.
Research Group in Human Reproductive Immunobiology, University of Cambridge, England.
G
G
Chumbley
A
A
King
N
N
Holmes
Y W
YW
Loke
D015152:Blotting, Northern; D015139:Blotting, Southern; D002478:Cells, Cultured; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D015345:Oligonucleotide Probes; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D014327:Trophoblasts / Q000378:metabolism*
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D015345:Oligonucleotide Probes; D012333:RNA, Messenger