Revival of Bacillus subtilis spores from biocide-induced injury in the germination process.
Spores of Bacillus subtilis NCTC 8236 were treated with glutaraldehyde, Lugol's iodine, polyvinylpyrrolidone-iodine (PVP-I), sodium hypochlorite or sodium dichloroisocyanurate (NaDCC). After exposure survivors were enumerated on nutrient agar containing potential revival agents (subtilisin, lysozyme, calcium dipicolinate, calcium lactate). Of these, only calcium lactate had any significant enhancing effect and then only with iodine-treated spores. Calcium lactate (9 mmol l-1) in nutrient broth enhanced the rate and extent of germination of iodine-treated spores but not of spores previously subjected to glutaraldehyde, hypochlorite or NaDCC.
Welsh School of Pharmacy, University of Wales College of Cardiff, UK.
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Williams
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Russell
D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology*; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D009113:Muramidase / Q000494:pharmacology; D010848:Picolinic Acids / Q000494:pharmacology; D011393:Procollagen-Proline Dioxygenase / Q000037:antagonists & inhibitors; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology; D013381:Subtilisins / Q000494:pharmacology; D013997:Time Factors
D004202:Disinfectants; D007773:Lactates; D010848:Picolinic Acids; D019344:Lactic Acid; D011393:Procollagen-Proline Dioxygenase; D009113:Muramidase; D013381:Subtilisins; C004860:dipicolinic acid