ELISA screening with GC-MS confirmation of the tranquilizer chlorprothixene administered in subtherapeutic doses to horses.
A commercially available generic promazine ELISA kit is available which shows cross-reactivity for the tranquilizer chlorprothixene (CPT). The ELISA test readily detects the presence of CPT or its metabolites in equine urine for up to 24 h after the i.v. and i.m. administration of sub-therapeutic doses (4.5 mg) to three horses. Maximum concentrations (CPT equivalents) are obtained 2 h after i.v. dosing. No distinct concentration peak values are observed after i.m. administration. Following solid-phase extraction, confirmation of CPT and its metabolites by electron impact mass spectrometry after sub-therapeutic administration is not successful. The use of chemical ionization mass spectrometry however revealed the presence of at least four metabolites including; chlorprothixene sulphoxide, hydroxylated chlorprothixene and hydroxylated chlorprothixene sulphoxide.
Vakgroep Farmacologie, Farmacie en Toxicolog., Faculteit Diergeneeskunde Universiteit Gent, Belgium.
F T
FT
Delbeke
P
P
Teale
M
M
Debackere
E
E
Houghton
D000818:Animals; D002749:Chlorprothixene / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000652:urine*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000662:veterinary*; D006736:Horses / Q000652:urine*; D006900:Hydroxylation; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D012015:Reference Standards
C037465:chlorprothixene sulfoxide; D002749:Chlorprothixene