Enantioselective enzymatic biotransformation of 2'-deoxy-3'-thiacytidine (BCH 189) monitored by capillary electrophoresis.
Concentrations of the enantiomers of 2'-deoxy-3'-thiacytidine (BCH 189) have been monitored during enantiospecific biotransformation catalyzed by cytidine deaminase. This enzymatic reaction is monitored by capillary electrophoresis (CE) over a 51-h time period. The use of dimethyl-beta-cyclodextrin as a chiral electrolyte additive in a pH 2.3 phosphate buffer system gives enantiomeric separation with results for peak efficiencies, resolution, and analysis time of the order of 25,000 plates, > 1.5 and 30 min, respectively. The features of the CE method are compared with those of the previously favored chiral HPLC method.
Rogan
M M
MM
Pharmaceutical Analysis Department, Glaxo Group Research, Ware, Hertfordshire, United Kingdom.
Drake
C
C
Goodall
D M
DM
Altria
K D
KD
D000998:Antiviral Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003505:Cyclodextrins; D003564:Cytidine Deaminase / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D005069:Evaluation Studies as Topic; D019259:Lamivudine; D013237:Stereoisomerism; D016047:Zalcitabine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D047392:beta-Cyclodextrins
D000998:Antiviral Agents; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D019259:Lamivudine; D016047:Zalcitabine; D003564:Cytidine Deaminase; C031215:betadex