Use of pyrenemethyl laurate for fluorescence-based determination of lipase activity in intact living lymphoblastoid cells and for the diagnosis of acid lipase deficiency.
Pyrenemethyl laurate (PMLes), a fluorogenic substrate for determining in vitro lipase activity [Nègre, Salvayre, Dagan and Gatt (1989) Biochim. Biophys. Acta 1006, 84-88], has been administered to cultured lymphoblastoid cells from normal subjects and from a patient affected with Wolman disease, which is characterized by a deficiency of lysosomal acid lipase. The intracellular degradation of PMLes was dependent on the mode of administration of the substrate into the cells, and occurred by two separate pathways involving lysosomal and extra-lysosomal hydrolases. PMLes incorporated into LDL was taken up by normal lymphoblastoid cells through the apolipoprotein-B/E-receptor-mediated pathway and degraded in the lysosomal compartment, as suggested by the degradation block in Wolman cells. In contrast, when PMLes dissolved in 2% dimethyl sulphoxide was added directly to the culture medium, its hydrolysis was similar in lymphoblastoid cells from controls and from patients affected with Wolman disease, neutral lipid storage disease or familial hypercholesterolaemia. This suggested that the administered PMLes was degraded by a non-lysosomal enzyme which is not deficient in Wolman cells. This enzyme also differs from the neutral lipase system which is deficient in lymphoblastoid cells from patients with neutral lipid storage disease. When pyrenemethanol was administered directly to the cell culture, it was only poorly acylated and was rapidly released into the culture medium. These results and the fluorescence properties of PMLes ('monomeric' emission in a hydrophobic environment and 'excimeric' emission in a hydrophilic environment) and pyrenemethanol ('monomeric' emission in a hydrophilic environment) allowed us to design a 'direct reading' procedure by monitoring (without any lipid extraction) the fluorescence of intact living cells and that of the culture medium during pulse-chase experiments. This method allowed the direct evaluation of the time course of in situ degradation of PMLes. In pulse-chase experiments with LDL-PMLes, the fluorescence of normal cells decreased relatively rapidly with time whereas the fluorescence of the culture medium increased concomitantly. With Wolman cells, the cellular fluorescence decreased only very slightly, whereas that of the culture medium remained at the basal level; this demonstrates the catabolic block in intact living cells from patients with Wolman disease. In vitro degradation of PMLes indicated the existence of two PMLes-degrading enzymes in lymphoblastoid cell homogenates: one is the acid lipase which is involved in PMLes degradation in the lysosomal compartment (and is deficient in Wolman cells), while the second is a cytoplasmic enzyme (not deficient in Wolman cells).
Department of Biochemistry, Faculty of Medicine in Rangueil, University Paul Sabatier, Toulouse, France.
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Nègre-Salvayre
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Dagan
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Gatt
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Salvayre
D001692:Biological Transport; D004705:Endocytosis; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D007848:Laurates; D008049:Lipase / Q000032:analysis* / Q000172:deficiency; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008214:Lymphocytes / Q000201:enzymology*; D008856:Microscopy, Fluorescence; D011721:Pyrenes; D011956:Receptors, Cell Surface / Q000378:metabolism; D018110:Receptors, Lipoprotein; D015223:Wolman Disease / Q000175:diagnosis*
D005456:Fluorescent Dyes; D007848:Laurates; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011721:Pyrenes; D011956:Receptors, Cell Surface; D018110:Receptors, Lipoprotein; C072413:apolipoprotein B receptor; C061646:pyrenemethyl laurate; D008049:Lipase