Neurotensin stimulates cyclic AMP formation in CHO-rNTR-10 cells expressing the cloned rat neurotensin receptor.
Recently, we reported that the rat neurotensin receptor expressed in transfected Chinese hamster ovary cells (CHO-rNTR-10) mediates the hydrolysis of polyphosphoinositides. In this study, we show that neurotensin stimulated cyclic AMP formation in CHO-rNTR-10 cells in a dose-dependent manner (EC50 = 34 nM). Neurotensin also stimulated cyclic AMP formation in membranal preparations under Ca(2+)-free conditions. These results suggest that this cyclic AMP formation occurs from direct coupling of the neurotensin receptor to a type of GTP binding protein, mediating synthesis of cyclic AMP, and not from an increase in intracellular Ca2+ concentration from polyphosphoinositide hydrolysis.
Department of Psychiatry and Psychology, Mayo Foundation, Jacksonville, FL 32224.
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Yamada
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Yamada
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Watson
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Richelson
D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D006224:Cricetinae; D000242:Cyclic AMP / Q000096:biosynthesis*; D006868:Hydrolysis; D009496:Neurotensin / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D010716:Phosphatidylinositols; D018028:Receptors, Neurotensin; D017981:Receptors, Neurotransmitter; D009496:Neurotensin; D000242:Cyclic AMP; D002118:Calcium