Antisense technology: biological utility and practical considerations.
Antisense RNA and DNA techniques have been developed as a relatively recent approach to the specific modulation of gene expression in vitro and in vivo. This review discusses general considerations for the application of antisense techniques. We shall examine the relative advantages and disadvantages of DNA versus RNA techniques, as well as the common pitfalls peculiar to each strategy.
Clinical Pharmacology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
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Neckers
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Whitesell
D000818:Animals; D016375:Antisense Elements (Genetics); D016207:Cytokines / Q000502:physiology; D016373:DNA, Antisense; D015870:Gene Expression; D006133:Growth Substances / Q000502:physiology; D006801:Humans; D015513:Oncogene Proteins; D009857:Oncogenes; D016372:RNA, Antisense; D011956:Receptors, Cell Surface / Q000378:metabolism
D016375:Antisense Elements (Genetics); D016207:Cytokines; D016373:DNA, Antisense; D006133:Growth Substances; D015513:Oncogene Proteins; D016372:RNA, Antisense; D011956:Receptors, Cell Surface