Conformational behavior of Escherichia coli OmpA signal peptides in membrane mimetic environments.
Nuclear magnetic resonance and circular dichroism (CD) studies of isolated peptides corresponding to WT and mutant OmpA signal sequences are reported; all of the peptides adopt substantial amounts of alpha-helical structure both in 1:1 (v/v) trifluoroethanol (TFE)/water and in sodium dodecyl sulfate (SDS) micelles. In TFE/water, the helix begins after the positively charged N-terminal residues and is most stable in the hydrophobic core, which correlates with results obtained previously for other signal sequences. The helix is weaker between the hydrophobic core and the C-terminus; such a break in the helix appears to be common to other signal peptides studied previously and could be of functional importance. No clear correlation could be established between the helicity of the peptides in TFE/water and their in vivo activities. All the peptides have a higher alpha-helix content in SDS than in TFE/water, and there is a good correlation between helix content in SDS and in vivo activity. Helicity in SDS for the functional peptides increases both at the N-terminus and in the hydrophobic core, and is driven by a strong association of the core with the hydrophobic chains of the detergent. The extension of the helix toward the N-terminus may be a result of neutralization of the N-terminal positive charges by the headgroups of the micelles, which removes unfavorable electrostatic interactions with the helix dipole. All these comparisons were facilitated by the use of upfield shifts of H alpha protons in helical regions relative to random coil chemical shifts, which also yielded estimates of helical content that correlated well with the CD results.
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9041.
J
J
Rizo
F J
FJ
Blanco
B
B
Kobe
M D
MD
Bruch
L M
LM
Gierasch
D000577:Amides / Q000737:chemistry; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D004252:DNA Mutational Analysis; D003903:Deuterium; D004926:Escherichia coli / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008969:Molecular Sequence Data; D011487:Protein Conformation; D021382:Protein Sorting Signals / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011522:Protons; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D014270:Trifluoroethanol / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000577:Amides; D001425:Bacterial Outer Membrane Proteins; D008823:Micelles; D021382:Protein Sorting Signals; D011522:Protons; D014867:Water; D012967:Sodium Dodecyl Sulfate; D014270:Trifluoroethanol; D003903:Deuterium