Aldehydes from metal ion- and lipoxygenase-induced lipid peroxidation: detection by 1H-n.m.r. spectroscopy.
The modification of lipoproteins by reactive aldehydes formed via lipid peroxidation is thought to be a key process in the pathogenesis of atherosclerosis. We show that 1H-n.m.r. spectroscopy can readily be used to detect a variety of different aldehydes resulting from peroxidation of liposomes induced by Fenton's reagent or lipoxygenase, and aldehydes arising from copper-induced reactions of low-density lipoprotein. There is a clear contrast between the major aldehydic products arising from metal-ion- and lipoxygenase-induced reactions.
Lodge
J K
JK
Department of Chemistry, Birkbeck College, University of London, U.K.
Patel
S U
SU
Sadler
P J
PJ
D000447:Aldehydes / Q000032:analysis*; D003300:Copper / Q000494:pharmacology*; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008081:Liposomes; D008084:Lipoxygenase / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D011522:Protons
D000447:Aldehydes; D008077:Lipoproteins, LDL; D008081:Liposomes; D011522:Protons; D003300:Copper; D008084:Lipoxygenase