Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1.
Arbitrarily primed polymerase chain reaction (AP-PCR) was used to characterize Legionella pneumophila serogroup 1. Cells from a single colony could be subtyped by AP-PCR within a few hours. The discrimination between strains of L. pneumophila serogroup 1 by AP-PCR was equivalent to that by monoclonal antibody analysis and ribotyping. Four strains representing the monoclonal antibody pattern most frequently associated with outbreaks all yielded unique amplicon patterns by AP-PCR.
Departamento de Microbiologia y Medicina Preventiva y Salud Publica, Universidad de Zaragoza, Spain.
P
P
Gomez-Lus
B S
BS
Fields
R F
RF
Benson
W T
WT
Martin
S P
SP
O'Connor
C M
CM
Black
D000911:Antibodies, Monoclonal; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D005069:Evaluation Studies as Topic; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics / Q000276:immunology; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology; D008969:Molecular Sequence Data; D012703:Serotyping
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes