[Studies of the homology of chromosomes between human being and rhesus monkey with chromosomal in situ suppression hybridization].
Chromosomal in situ suppression (CISS) hybridization of biotin-labeled DNA libraries for human chromosomes 1, 2 and 4 was used to investigate chromosome homology between human being and rhesus monkey. The results demonstrate that chromosome 1 in human being and rhesus monkey is homologous, of which the 1pter-->1q33 of rhesus monkey is highly homologous with a cognate region of chromosome 1 in human, while the 1q33-->1qter is relatively low; Chromosome 2 in human shows homology with the long arm of chromosome 13 as well as the long arm and the partial short arm of chromosome 9 in the rhesus, and chromosome 4 in human is identical to chromosome 2 in rhesus monkey. Combined with a comparative analysis of banding patterns of chromosomes, derivation relationships of chromosomes between man and the rhesus are discussed. Our present data provide a definitive proof that chromosomal rearrangements may be a major mechanism of chromosomal evolution in primates.
Department of Biology, Nanjing Normal University.
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Huang
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Zhang
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Chen
D000818:Animals; D005075:Biological Evolution; D002871:Chromosome Banding; D002875:Chromosomes; D002877:Chromosomes, Human; D006801:Humans; D017403:In Situ Hybridization; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male