Automated sample clean-up with solid-phase extraction for the determination of aflatoxins in urine by liquid chromatography.
An automated extraction and clean-up procedure was developed for the determination of aflatoxins in human urine at the 50 pg/ml level. Aflatoxins B1, B2, G1 and G2 are captured on C2 extraction columns and simultaneously cleaned up with the aid of a robotic system. The processed samples are analysed by reversed-phase high-performance liquid chromatography. Fluorescence detection was enhanced for aflatoxins B1 and G1 using factorial design optimization of the post-column reactor. Silylation of the glass vials used in the robotic system was of the utmost importance. With non-silylated glass vials, up to 75% of the analytes were lost. Average aflatoxin recoveries were B1 95%, B2 90%, G1 93% and G2 89%.
National Institute of Occupational Health, Analytical Chemistry Division, Umeå, Sweden.
A
A
Kussak
B
B
Andersson
K
K
Andersson
D000348:Aflatoxins / Q000652:urine*; D001322:Autoanalysis; D001966:Bromine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D012015:Reference Standards; D012371:Robotics; D012821:Silanes / Q000032:analysis; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; D007202:Indicators and Reagents; D012821:Silanes; D001966:Bromine