Protein and enzyme content of plasma membranes derived from Walker 256 carcinoma cells grown as ascitic or solid tumours.
A preliminary investigation of enzymes functioning at the cell surface indicates that when Walker 256 carcinoma cells growing ascitically are induced to grow as a solid tumour there is a marked increase in the activities of Na+, K(+)-ATPase, Mg(2+)-ATPase, 5'-nucleotidase, alkaline phosphatase and phosphodiesterase I; 5'-nucleotidase and alkaline phosphatase being particularly affected. These enzyme changes occur in the absence of any major alteration in the protein composition of the plasma membrane.
School of Biological and Medical Sciences, Division of Cell and Molecular Biology, University of St. Andrews, Fife, Scotland, UK.
C M
CM
Clark
G A
GA
Goodlad
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001202:Ascitic Fluid; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002279:Carcinoma 256, Walker / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D046508:Culture Techniques; D005260:Female; D008565:Membrane Proteins / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009368:Neoplasm Transplantation; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012878:Skin Neoplasms; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D008565:Membrane Proteins; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D009699:N-Glycosyl Hydrolases; C030681:purine nucleosidase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase