Function of gene 49 of bacteriophage T4 III. Isolation of Holliday structures from very fast-sedimenting DNA.
Branched DNA molecules were identified in ClaI digests of cytosine containing very fast-sedimenting DNA (VFS-DNACYT) which was isolated from Escherichia coli infected with the multiple mutant 49-GT7 of phage T4. In about 10% of randomly picked ClaI fragments branches with three arms (Y-structures) as well as four arms (Holliday structures) were seen in the electron microscope. Branched structures were absent from ClaI digests after treatment with purified endonuclease VII (gp49) in vitro.
Flemming
M
M
Institute for Genetics, Köln, Germany.
Deumling
B
B
Kemper
B
B
D017122:Bacteriophage T4 / Q000235:genetics*; D004279:DNA, Viral / Q000378:metabolism / Q000648:ultrastructure*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D009690:Nucleic Acid Conformation
D004279:DNA, Viral; C032824:ATCGAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific