Two-way cross-neutralization mediated by a shared P (VP4) serotype between bovine rotavirus strains with distinct G (VP7) serotypes.
Among bovine rotavirus strains, there are three G serotypes (G6, G8, and G10) and three P (VP4) serotypes (PB1, PB2, and PB3, which are defined on the basis of strains NCDV, UK, and B223, respectively). Plaque reduction neutralization assays with hyperimmune antisera disclosed two-way antigenic relationships of strain KN-4 with strain KK-3 (G10, PB3) as well as with strains NCDV (G6, PB1) and 0510 (G6, PB2). Neutralization assays with monoclonal antibodies specific for G6, G10, and PB3 revealed that KK-3 and KN-4 had the same P serotype (PB3) but that neither G6- nor G10-specific monoclonal antibody neutralized KN-4. Comparison of the VP7 gene sequence of KN-4 with those of other bovine rotavirus strains indicated that KN-4 was more similar to G6 bovine strains than to KK-3 and other G10 strains, suggesting that the G serotype of KN-4 was G6. From these results, we concluded that the two-way cross-neutralization between KN-4 and NCDV or 0510 was mediated by shared G6 serotype specificity, whereas the two-way cross-neutralization between KN-4 and KK-3 was mediated by shared P serotype specificity (PB3). Thus, KN-4 and KK-3 represent the first reported example of a two-way antigenic relationship mediated only by the P serotype. This article emphasizes the need for adopting a binary serotyping system and development of reagents which will enable classification of rotaviruses based on their G and P serotype specificities.
Matsuda
Y
Y
Animal Facilities for Experimental Medicine, Akita University School of Medicine, Japan.
Isegawa
Y
Y
Woode
G N
GN
Zheng
S
S
Kaga
E
E
Nakagomi
T
T
Ueda
S
S
Nakagomi
O
O
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins; D002417:Cattle; D003429:Cross Reactions; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D013045:Species Specificity
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus