Reduced expression of PLC-gamma during the differentiation of mouse F9 teratocarcinoma cells.
Expression of phosphoinositide-specific phospholipase C (PLC)-gamma during the retinoic acid (RA)-induced differentiation of F9 teratocarcinoma stem cells was investigated. PLC-gamma activity and PLC-gamma protein content were decreased during the differentiation of F9 teratocarcinoma stem cells induced by a combination (RACT) of RA, dibutyryl cyclic AMP (C) and theophylline (T). However, PLC-gamma activity and protein levels in RA- or C- and T-treated F9 cells remained the same as that of the F9 stem cells. Northern analysis of the 5.4-kb PLC-gamma transcript in differentiated F9 cells by RACT revealed that RACT decreased the PLC-gamma gene expression. These results suggest that the expression of PLC-gamma is negatively controlled by RACT treatment in the differentiation of F9 teratocarcinoma stem cells.
Department of Life Science, Pohang Institute of Science and Technology, Korea.
Y H
YH
Lee
H Y
HY
Lee
S H
SH
Ryu
P G
PG
Suh
K W
KW
Kim
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis*; D013724:Teratoma / Q000378:metabolism* / Q000473:pathology; D013806:Theophylline / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D014212:Tretinoin; D003994:Bucladesine; D013806:Theophylline; D010727:Phosphoric Diester Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase