Differential changes in CNS phosphatase activities during seizures.
Neutral and alkaline phosphatase activities were studied in small synaptosomes isolated from rat brain and cerebellum after administration of the convulsant 3-mercaptopropionic acid (MP). Cerebral cortex phosphatase activity assayed at pH 7.2 in the presence of K+ increased both during and after seizures; when it was assayed at pH 7.2 in the absence of K+ it decreased at both stages. The enzyme activity determined in cortical fractions at pH 9.0 with or without K+ decreased in seizure and postseizure states. After MP treatment, cerebellum enzyme activity assay at pH 7.2 was higher than in controls throughout; phosphatase activity assayed at pH 9.0 decreased following MP administration except during seizures when the enzyme was assayed in the presence of K+. Such changes following MP treatment may well be related to seizure-induced alteration of phosphate ester levels or protein-kinase activities.
Rodriguez de Lores Arnaiz
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Instituto de Biología Celular, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Girardi
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Pérez Raffo
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D015097:3-Mercaptopropionic Acid / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002490:Central Nervous System / Q000201:enzymology*; D002531:Cerebellum / Q000187:drug effects / Q000201:enzymology; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000201:enzymology*; D013574:Synaptosomes / Q000187:drug effects / Q000201:enzymology
D015097:3-Mercaptopropionic Acid; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases