Relaxation of imprinted genes in human cancer.
Genomic imprinting, or parental allele-specific expression of genes, has been demonstrated at the molecular level in insects and mice but not in man. Imprinting as a potential mechanism of human disease is suggested by paternal uniparental disomy of 11p15 in Beckwith-Wiedemann syndrome and by maternal uniparental disomy of 15q11-12 in Prader-Willi syndrome. Beckwith-Wiedemann syndrome is characterized by multiorgan overgrowth and predisposition to embryonal tumours such as Wilms' tumour of the kidney. A loss of heterozygosity of 11p15 is also frequently found in a wide variety of tumours, including Wilms' tumour and lung, bladder, ovarian, liver and breast cancers; 11p15 also directly suppresses tumour growth in vitro. Two genes in this band, H19 and insulin-like growth factor-II (IGF2) undergo reciprocal imprinting in the mouse, with maternal expression of H19 (ref. 13) and paternal expression of IGF2 (ref. 14). Here we find that both of these genes show monoallelic expression in human tissues and, as in mouse, H19 is expressed from the maternal allele and IGF2 from the paternal allele. In contrast, 69% of Wilms' tumours not undergoing loss of heterozygosity at 11p showed biallelic expression of one or both genes, suggesting that relaxation or loss of imprinting could represent a new epigenetic mutational mechanism in carcinogenesis.
Rainier
S
S
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109.
Johnson
L A
LA
Dobry
C J
CJ
Ping
A J
AJ
Grundy
P E
PE
Feinberg
A P
AP
D000483:Alleles; D001483:Base Sequence; D004277:DNA, Single-Stranded; D004303:Dosage Compensation, Genetic; D005220:Fathers; D005260:Female; D005333:Fetus; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D007680:Kidney Neoplasms / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009035:Mothers; D009369:Neoplasms / Q000235:genetics*; D010375:Pedigree; D009396:Wilms Tumor / Q000235:genetics
D004277:DNA, Single-Stranded; D007335:Insulin-Like Growth Factor II