Parainfluenza virus type 3 (PIV3)-specific and non-virus-specific delayed type hypersensitivity responses in cotton rats given different PIV3 antigen preparations.
Virus-specific, T-lymphocyte-mediated delayed type hypersensitivity (DTH) responses were studied in cotton rats using replicating (wild-type parainfluenza virus type 3 (PIV3) and recombinant vaccinia virus expressing PIV3 haemagglutinin-neuraminidase (HN) or fusion (F) glycoproteins), and non-replicating (detergent-solubilized, affinity chromatography purified HN and F glycoproteins or inactivated whole PIV3) virus preparations. Significant virus-specific DTH responses were observed in all test groups 1-2 weeks after a single antigen inoculation or 5 days after two inoculations given 3 weeks apart. Peak swelling of ear pinnas in these animals generally occurred 24 h after elicitation and was marked by a cellular infiltration consisting of mono- and polymorphonuclear leucocytes. A considerable non-virus-specific inflammatory response, presumably due to tissue culture or media components in the priming antigen preparations, was observed 3 weeks after priming. No significant differences in DTH responses were observed in cotton rats primed with any of the PIV3 preparations. The possible roles and significance of both the virus-specific and non-virus-specific DTH responses in paramyxovirus-induced disease in humans and cotton rats are discussed.
Ambrose
M W
MW
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030.
Wyde
P R
PR
D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage* / Q000302:isolation & purification; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000473:pathology; D007116:Immunization, Passive; D007700:Kinetics; D010224:Parainfluenza Virus 3, Human / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D016205:Sigmodontinae; D015164:Vaccines, Inactivated / Q000008:administration & dosage; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000302:isolation & purification; D014765:Viral Vaccines / Q000008:administration & dosage / Q000302:isolation & purification; D014779:Virus Replication
D000956:Antigens, Viral; D015164:Vaccines, Inactivated; D014614:Vaccines, Synthetic; D014765:Viral Vaccines