Diagnosis of active urinary schistosomiasis using homologous adult Schistosoma haematobium antigen.
The role of antigen prepared from Schistosoma haematobium adult worm (ShAWA, W.H.O. Geneva) in diagnosis of active urinary schistosomiasis, by counterimmunoelectrophoresis (CIEP), was evaluated by comparison with simpler methods as sedmentation of urinary ova and detection of haematuria by chemical reagent strips. Sixty patients (30 school boys and 30 adults) passing living S. haematobium eggs, as detected, by nucleopore filtration technique (NF), and 30 controls were studied. Results showed statistically significantly higher (P < 0.001) egg counts in school boys than adults. Sensitivities of haematuria detection, ova sedimentation and CIEP were 93.3%, 80%, 50%, 70%, 73.3%, 33.3% in school boys and adults respectively. It was concluded that direct parasitological examination of urine samples is more superior than CIEP using homologous ShAWA to detect active urinary schistosomiasis both qualitatively and quantitatively. Haematuria detected by chemical reagent strips can identify a high proportion of active urinary schistosomiasis in epidemiological surveys in endemic areas. Nucleopore filtration is more superior than ova sedimentation method for detection of active urinary schistosomiasis.
Department of Parasitology, Faculty of Medicine, Ain Shams University Cairo, Egypt.
H M
HM
Khalil
M H
MH
Abdel Baki
H M
HM
Abdallah
E A
EA
el Zayyat
S S
SS
Abdel Aziz
D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth; D002648:Child; D003378:Counterimmunoelectrophoresis / Q000379:methods*; D005069:Evaluation Studies as Topic; D006417:Hematuria / Q000175:diagnosis; D006801:Humans; D008297:Male; D012548:Schistosoma haematobium / Q000276:immunology* / Q000302:isolation & purification; D012553:Schistosomiasis haematobia / Q000175:diagnosis*; D014556:Urine / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth