Diet during rotavirus enteritis affects jejunal permeability to macromolecules in suckling rats.
We studied the influence of diet during diarrhea on gut mucosal barrier in a suckling rat model. Rat pups were inoculated with IDIR virus (a group B rotavirus) at 10 d of age. Beginning 2 d postinfection, in addition to maternal milk, group CM received a daily gavage of cow milk and group GG received Lactobacillus casei strain GG, a human strain previously shown to survive the passage through the gastrointestinal tract and temporarily colonize the gut. Group CMGG received a combination of these, and control animals were gavaged with tap water. At 21 d of age, jejunal absorption of intact and degraded horseradish peroxidase (HRP) in Ussing chamber was markedly higher in IDIR virus-infected than in noninfected controls. In the two groups gavaged with cow milk, group CM and group CMGG, the numbers of specific antibody-secreting cells (enumerated by the solid-phase enzyme-linked immunospot assay) against beta-lactoglobulin were significantly higher than in the groups that had not received cow milk. In parallel with immune system activation, a statistically significant increase in the absorption of intact HRP (mean and 95% confidence interval, ng x h-1 x cm-2) was detected: group CM, 302 (155, 586); group CMGG, 174 (56, 545); infected controls, 121 (57, 257); and group GG, 44 (8, 254). A decrease in the uptake of intact HRP (F = 3.64, p = 0.06) and degraded HRP (F = 9.50, p = 0.004) was associated with the introduction of L. casei GG to the diet, irrespective of coexposure to cow milk.(ABSTRACT TRUNCATED AT 250 WORDS)
Isolauri
E
E
University of Tampere, Department of Clinical Sciences, Finland.
Kaila
M
M
Arvola
T
T
Majamaa
H
H
Rantala
I
I
Virtanen
E
E
Arvilommi
H
H
D000818:Animals; D000833:Animals, Suckling; D000921:Antibody-Producing Cells / Q000276:immunology; D004032:Diet; D005260:Female; D005516:Food Microbiology; D005759:Gastroenteritis / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology; D007583:Jejunum / Q000378:metabolism* / Q000382:microbiology; D007780:Lacticaseibacillus casei / Q000502:physiology; D007782:Lactoglobulins / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar; D012400:Rotavirus Infections / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism
D007782:Lactoglobulins; D006735:Horseradish Peroxidase