Metabolic effects of benzoate and sorbate in the yeast Saccharomyces cerevisiae at neutral pH.
Preincubation of yeast cells in the presence of benzoate or sorbate at an extracellular pH value of 6.8 elicited a set of metabolic effects on sugar metabolism, which became apparent after the subsequent glucose addition. They can be summarized as follows: a) reduced glucose consumption; b) inhibition of glucose- and fructose-phosphorylating activities; c) suppression of glucose-triggered peak of hexoses monophosphates; d) substantial reduction of glucose-triggered peak of fructose 2,6-bisphosphate; e) block of catabolite inactivation of fructose-1,6-bisphosphatase and phosphoenolpyruvate carboxy-kinase, but not of cytoplasmic malate dehydrogenase. On the whole this pattern resulted in prevention of glucose-induced switch of metabolism from a gluconeogenetic to a glycolytic state. Our data also show that, unlike former assumptions, intracellular acidification is not likely to mediate the bulk of metabolic effects of benzoate and sorbate, since under our working conditions intracellular pH kept close to neutrality.
Dipartimento di Fisiologia e Biochimica generali, Università di Milano, Italy.
N
N
Burlini
R
R
Pellegrini
P
P
Facheris
P
P
Tortora
A
A
Guerritore
D001565:Benzoates / Q000494:pharmacology*; D019817:Benzoic Acid; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005635:Fructosediphosphates / Q000378:metabolism; D005943:Gluconeogenesis / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D013011:Sorbic Acid / Q000494:pharmacology*
D001565:Benzoates; D005635:Fructosediphosphates; C027652:fructose 2,6-diphosphate; D019817:Benzoic Acid; D006597:Fructose-Bisphosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D005947:Glucose; D013011:Sorbic Acid