Marker rescue of a transformation-negative Epstein-Barr virus recombinant from an infected Burkitt lymphoma cell line: a method useful for analysis of genes essential for transformation.
A Burkitt lymphoma cell line infected in vitro with a transformation-defective mutant recombinant Epstein-Barr virus (EBV) was used to attempt marker rescue of transformation competence by transfection with cloned wild-type DNA. EBV replication was induced in the transfected cells, and wild-type EBV DNA recombined via flanking homologous sequences adjacent to the deletion, resulting in a virus which transformed primary B lymphocytes in vitro. This strategy should be useful for molecular genetic analysis of the role of part or all of any gene in cell growth transformation.
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115.
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A
Marchini
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E
Kieff
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R
Longnecker
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000382:microbiology; D002051:Burkitt Lymphoma / Q000382:microbiology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D015870:Gene Expression; D005819:Genetic Markers; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D014162:Transfection / Q000235:genetics; D014407:Tumor Cells, Cultured
D005819:Genetic Markers