MxA-dependent inhibition of measles virus glycoprotein synthesis in a stably transfected human monocytic cell line.
The alpha/beta (type I) interferon-inducible human MxA protein confers resistance to vesicular stomatitis virus (VSV) and influenza A virus in MxA-transfected mouse 3T3 cells (3T3/MxA). We investigated the inhibitory effects of the MxA protein on measles virus (MV) and VSV in the human monocytic cell line U937. In transfected U937 clones which constitutively express MxA (U937/MxA), the release of infectious MV and VSV was reduced approximately 100-fold in comparison with control titers. Transcription of VSV was inhibited similar to that observed for 3T3/MxA cells, whereas no difference was detected for MV in the rates of transcription or the levels of MV-specific mRNAs. In contrast, analysis of MV protein expression by immunofluorescence and immunoprecipitation revealed a significant reduction in the synthesis of MV glycoproteins F and H in U937/MxA cells. These data demonstrate a virus-specific effect of MxA which may, in the case of MV, contribute to the establishment of a persistent infection in human monocytic cells.
Institute for Virology and Immunobiology, Würzburg, Germany.
J J
JJ
Schnorr
S
S
Schneider-Schaulies
A
A
Simon-Jödicke
J
J
Pavlovic
M A
MA
Horisberger
V
V
ter Meulen
D016475:3T3 Cells; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D015152:Blotting, Northern; D002460:Cell Line; D019204:GTP-Binding Proteins; D006801:Humans; D008459:Measles virus / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D009000:Monocytes; D064547:Myxovirus Resistance Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000096:biosynthesis*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D014779:Virus Replication
D000998:Antiviral Agents; C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D011506:Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D019204:GTP-Binding Proteins