Evaluation of isolator caging systems for protection of mice against challenge with mouse hepatitis virus.
Two isolator caging systems were evaluated against challenge with MHV-Y, an enterotropic strain of mouse hepatitis virus. The systems were similar in that they both used an identical shoebox cage equipped with a polycarbonate filter top incorporating a Reemay filter. They differed in that one system supplied HEPA-filtered air through a grommet in the filter lid so that the cage was pressurized slightly. A rack holding 60 cages (30 front and back) was utilized. Thirty cages without filter tops housed one mouse each that had been infected orally with 19,000 ID50 of MHV-Y and an uninfected cagemate. The remaining 30 cages, each housing 2 uninfected mice were divided into 3 groups of 10 cages. Group I cages (controls) had no filter top; Group II cages were equipped with filter tops; and Group III were equipped with filter tops and intracage HEPA-filtered air. The cages housing uninfected mice were interspersed between, above, below and behind cages housing infected mice. The uninfected mice were maintained in contact with the MHV-Y infected mice for 8 weeks. Transmission of MHV-Y was determined serologically by indirect ELISA. All mice housed within the Group I cages (control) seroconverted to MHV, while only 4 mice (2 cages) seroconverted in Group II, and no mice seroconverted in Group III.
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139.
N S
NS
Lipman
B F
BF
Corning
M
M
Saifuddin
D000818:Animals; D000830:Animals, Laboratory; D006524:Hepatitis, Viral, Animal / Q000517:prevention & control*; D006799:Housing, Animal; D051379:Mice; D008813:Mice, Inbred ICR; D006517:Murine hepatitis virus / Q000472:pathogenicity*; D013047:Specific Pathogen-Free Organisms