Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase.
Human cytochrome c oxidase (COX) is a complex of 13 subunits: three are encoded by mitochondrial DNA and ten by nuclear DNA. We have now isolated a full-length cDNA specifying subunit VIIb, the last remaining uncharacterized nuclear-encoded subunit cDNA of human COX. The cDNA encodes a deduced 80-aa polypeptide, including a 24-amino acid (aa) N-terminal leader sequence and a 56-aa mature polypeptide with 82% identity to mature bovine COX VIIb. Southern blot hybridization of human muscle genomic DNA showed multiple hybridizing bands, implying the presence of a large coxVIIb gene family, including a potential processed pseudogene.
Department of Neurology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
J E
JE
Sadlock
R N
RN
Lightowlers
R A
RA
Capaldi
E A
EA
Schon
D000595:Amino Acid Sequence; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D012333:RNA, Messenger; D003576:Electron Transport Complex IV