Examination of osteoblast-orthopaedic biomaterial interactions using molecular techniques.
Molecular techniques can be used to elucidate the effects of extended periods of cell-biomaterial interactions on the time-course and level of expression of particular genes which determine cellular phenotype. We used the polymerase chain reaction to demonstrate the expression of genes for the bone-related proteins osteocalcin, osteonectin and osteopontin by neonatal rat calvarial osteoblasts. In addition, Northern blotting was subsequently used to show that messenger RNAs encoding osteonectin and osteopontin were consistently expressed during a 5 wk period of interaction of osteoblasts with Ti-6Al-4V, a commercial brand of hydroxyapatite, and tissue culture polystyrene.
Puleo
D A
DA
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590.
Preston
K E
KE
Shaffer
J B
JB
Bizios
R
R
D000497:Alloys / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials / Q000494:pharmacology*; D015152:Blotting, Northern; D002478:Cells, Cultured; D017886:Durapatite; D015870:Gene Expression; D006882:Hydroxyapatites / Q000494:pharmacology; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000378:metabolism*; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics*; D015676:Osteonectin / Q000096:biosynthesis / Q000235:genetics*; D053495:Osteopontin; D016133:Polymerase Chain Reaction; D011137:Polystyrenes / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics*; D014025:Titanium / Q000494:pharmacology
D000497:Alloys; D001672:Biocompatible Materials; D006882:Hydroxyapatites; D015676:Osteonectin; D011137:Polystyrenes; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D015675:Osteocalcin; D053495:Osteopontin; C031462:titanium alloy (TiAl6V4); D017886:Durapatite; D014025:Titanium