Immunohistochemical detection of swine influenza A virus in formalin-fixed and paraffin-embedded tissues.
An avidin-biotin complex (ABC) immunohistochemical method utilizing a commercially-available polyclonal antiserum to human influenza A virus was used to detect antigens of influenza A virus in formalin-fixed, paraffin-embedded tissues of swine. Influenza A antigens were immunohistochemically detected in 28/30 cases in which influenza A virus was demonstrated by virus isolation and in 5/22 cases suspected to be influenza A-infected by clinical and histological criteria, but from which the virus was not isolated. Viral antigen was not demonstrated in 30/30 cases not suspected clinically or histologically to be associated with influenza infection. This method is a convenient, sensitive, and specific means of influenza A virus detection and is applicable to both routine diagnosis of influenza A virus infection and to retrospective and prospective studies of the occurrence and the pathogenesis of this virus in pigs.
Haines
D M
DM
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
Waters
E H
EH
Clark
E G
EG
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D008168:Lung / Q000382:microbiology; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
D000956:Antigens, Viral; D007106:Immune Sera; D001360:Avidin; D001710:Biotin