Molecular cloning of a human beta 3-adrenergic receptor cDNA.
We report the molecular cloning of a beta 3-adrenergic receptor [beta 3-AR] cDNA from human brown adipose tissue. The cDNA-encoded protein is identical to the previously cloned beta 3-AR but with 6 additional amino acids at the C-terminus. The C-terminus is shared by the beta 3 receptors expressed in human neuroblastoma cells [SK-N-MC] [Mol. Pharmacol. 42 (1992) 964-970]. Furthermore, using a polymerase chain reaction strategy we have cloned and sequenced the beta 3-AR introns. Sequence analysis demonstrates that the human beta 3-AR gene comprises at least 3 exons and 2 introns and that the most abundant beta 3-AR transcripts encode a protein with an exon 3-derived C-terminus. Interestingly, although a similar organization has been found in rodent genes, the rat beta 3-AR transcripts encode a receptor with an exon 2-derived C-terminus.
Laboratoire de Biologie Moleculaire, Sanofi Elf Biorecherches, Labege, France.
J M
JM
Lelias
M
M
Kaghad
M
M
Rodriguez
P
P
Chalon
J
J
Bonnin
I
I
Dupre
B
B
Delpech
M
M
Bensaid
G
G
LeFur
P
P
Ferrara
D002001:Adipose Tissue, Brown; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013566:Sympathomimetics / Q000378:metabolism*
D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D011994:Recombinant Proteins; D013566:Sympathomimetics