Effects of experimentally induced hyperammonemia on glial fibrillary acidic protein (GFAP) in the rhombencephalon of goldfish (Carassius auratus L.).
This experimental study was made to know the effect of hyperammonemia on glial fibrillary acidic protein (GFAP) in the glial cells of posterior rhombencephalon in the goldfish (Carassius auratus L.). Hyperammonemia was induced by elevating the ammonia concentration in the tank water to 0.88 mM with ammonium chloride; the ammonia level in the control tank water was < 0.1 mM. The GFAP levels were measured at 8, 16, 30, 60, 90 and 120 days. GFAP was quantified with a digital analysis system and a transient heterogeneous decrease of GFAP was observed. Hyperammonemia mostly affected GFAP in the astrocyte processes associated with cholinergic pathways. An explanation for the adaptive response to hyperammonemia by fish astrocytes is suggested.
Dpto. de Biología Celular y Genética, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
M
M
Rubio
G
G
Bodega
I
I
Suárez
R M
RM
Villalba
B
B
Fernández
D000641:Ammonia / Q000097:blood*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001330:Electronic Data Processing; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D007150:Immunohistochemistry; D010275:Parasympathetic Nervous System / Q000378:metabolism; D012249:Rhombencephalon / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
D005904:Glial Fibrillary Acidic Protein; D000641:Ammonia