Alteration of location of dimer linkage sequence in retroviral RNA: little effect on replication or homologous recombination.
Retrovirus particles contain a dimer of retroviral genomic RNA. A defined region of the retrovirus genome has previously been shown to be important for both dimerization and encapsidation. To study the importance of the position of this encapsidation and dimerization signal for retroviral replication and homologous recombination, we used a previously described spleen necrosis virus-based helper cell system. This system allows retroviral vectors with multiple genetic markers to be studied after a single cycle of retroviral replication. The sequence responsible for dimerization, the encapsidation/dimer linkage sequence (E/DLS), was moved from its normal location near the 5' end of the retroviral genome to a location near the 3' end of the genome. We characterized four pairs of retroviral vectors: (i) with both E/DLSs at the 5' ends of the genomes, (ii) with both E/DLSs at the 3' ends of the genomes, and (iii) two with one E/DLS at the 5' end of the genome and one at the 3' end of the genome. We found that moving the E/DLS to the 3' end of the genome resulted in at most an approximately factor of 5 reduction in virus titer in a single cycle of retroviral replication. Furthermore, we found no changes that were attributable to the alteration of the position of the E/DLS in the minus-strand DNA primer transfers or the plus-strand DNA primer transfers, the rate of homologous recombination, or the number of internal template switches in recombinant proviruses. These results indicate that any alignment or conformation necessary for retroviral replication or recombination is not the result of the position of the E/DLS.
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
J S
JS
Jones
R W
RW
Allan
H M
HM
Temin
D000818:Animals; D002874:Chromosome Mapping; D004285:Dogs; D005822:Genetic Vectors / Q000235:genetics; D011533:Proviruses / Q000235:genetics; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000254:growth & development*; D006377:T-Lymphocytes, Helper-Inducer; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D012330:RNA, Double-Stranded; D012367:RNA, Viral