ATP specifically bound as a hapten to a monoclonal anti-phospholipid antibody retains phosphate donor activity.
We have previously reported that each of four monoclonal antibodies to a phospholipid, phosphatidylinositol phosphate (PIP), has a phosphate binding subsite in the antigen binding site that can bind ATP (Molec. Immunol. 21, 863-868, 1984). We have now observed that antibody-bound ATP has the ability to donate a phosphate group in the phosphorylation reaction of glucose to glucose-6-phosphate catalyzed by hexokinase. The phosphorylation reaction proceeds equally efficiently when ATP is provided as free (nonbound) ATP or as antibody-bound ATP. We conclude that an anti-phospholipid antibody can serve as a carrier of a functionally active nucleotide.
Wassef
N M
NM
Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Swartz
G M
GM
Alving
B M
BM
Alving
C R
CR
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D017152:Antibodies, Antiphospholipid / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001665:Binding Sites; D005947:Glucose / Q000378:metabolism; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D006241:Haptens / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010710:Phosphates / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation
D017152:Antibodies, Antiphospholipid; D000911:Antibodies, Monoclonal; D005958:Glucosephosphates; D006241:Haptens; D008081:Liposomes; D010710:Phosphates; D010716:Phosphatidylinositols; D019298:Glucose-6-Phosphate; D000255:Adenosine Triphosphate; D006593:Hexokinase; D005947:Glucose