Muscle-specific transcriptional activation by CArG box requires either homophilic or heterophilic interactions of the CArG box binding factors.
CArG boxes (CC(A/T)6GG sequences) are present in various promoters and are able to confer two different types of transcriptional responsiveness: serum inducibility and muscle-specific activation. Inserted upstream from the ubiquitous HSV thymidine kinase promoter, multimerized HCA1 boxes (human cardiac alpha-actin proximal CArG box) behave as strong muscle-specific activating elements. Transient expression assay was used to determine whether the muscle-specific transcriptional activation by the CArG boxes depends on the presence in the vicinity of other specific cis-acting DNA elements. Our results show that no specific association between different regulatory binding sites is required for the myogenic activity of a CArG box and that CArG elements are able to stimulate transcription in myogenic cells through either homophilic or heterophilic interactions of the CArG box binding factors.
ICGM, U129, INSERM, Paris, France.
M
M
Soulez
D
D
Tuil
A
A
Kahn
F
F
Phan-Dinh-Tuy
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002999:Clone Cells; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007739:L Cells; D008113:Liver Neoplasms; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D000199:Actins; D004268:DNA-Binding Proteins; D013937:Thymidine Kinase