Experimental reactivation of HSV-I in rat vestibular ganglia.
Rats with no clinical symptoms after inoculation were administered with cyclophosphamide in order to reactivate HSV-I in the vestibular ganglia. After this immunosuppression, the vestibular ganglia, trigeminal ganglia, cerebrum, cerebellum and brainstem were examined immunohistologically in order to detect HSV-I. HSV-I antigen could not be detected by using indirect immunofluorescence or the ABC method, however, it could be detected by using the PCR method. In this study, latent infection of HSV-I was shown but reactivation could not be established. We need further investigations to determine the reactivation of HSV-I in the vestibular ganglia, to obtain an animal model of vestibular neuronitis.
Department of Otolaryngology, Yamaguchi University School of Medicine, Ube, Japan.
H
H
Okazaki
T
T
Sekitani
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D004292:Dominance, Cerebral / Q000502:physiology; D007108:Immune Tolerance / Q000187:drug effects / Q000276:immunology; D008297:Male; D008575:Meniere Disease / Q000276:immunology / Q000382:microbiology*; D009443:Neuritis / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D004856:Postural Balance / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018139:Simplexvirus / Q000254:growth & development*; D014725:Vestibular Nerve / Q000276:immunology / Q000382:microbiology*; D000160:Vestibulocochlear Nerve Diseases / Q000276:immunology / Q000382:microbiology*; D014775:Virus Activation / Q000276:immunology*
D003520:Cyclophosphamide