Tissue distribution of beta 3-adrenergic receptor mRNA in man.
Expression of mRNA for beta 1-, beta 2-, and beta 3-adrenergic receptors (beta 1-, beta 2-, and beta 3-AR) was investigated in human tissues. beta 1- and beta 2-AR mRNA distribution correlated with that of the cognate receptors established by pharmacological studies. beta 3-AR transcripts were abundant in infant perirenal brown adipose tissue, characterized by the presence of uncoupling protein (UCP) mRNA. In adult whole adipose tissues, beta 3-AR mRNA levels were high in deep deposits such as perirenal and omental, and lower in subcutaneous. In these deposits, UCP mRNA levels paralleled those of beta 3-AR. However, isolated omental and subcutaneous adipose cells, enriched in white adipocytes, expressed beta 3-AR but no UCP transcripts. beta 3-AR mRNA was highly expressed in gallbladder, and to a much lower extent in colon, independently of UCP mRNA. Quadriceps or abdominal muscles, heart, liver, lung, kidney, thyroid, and lymphocytes did not express intrinsic beta 3-AR mRNA. This study demonstrates that substantial amounts of brown adipocytes exist throughout life in adipose deposits, which are generally classified as white. These deposits are the main sites of beta 3-AR expression, which also occurs in gallbladder and colon. beta 3-AR may thus be involved in the control of lipid metabolism, possibly from fat assimilation in the digestive tract, to triglyceride storage and mobilization in adipose tissues.
Centre National de la Recherche Scientifíque-Unité Propre de Recherche 0415, Paris, France.
S
S
Krief
F
F
Lönnqvist
S
S
Raimbault
B
B
Baude
A
A
Van Spronsen
P
P
Arner
A D
AD
Strosberg
D
D
Ricquier
L J
LJ
Emorine
D000273:Adipose Tissue / Q000502:physiology; D000328:Adult; D000368:Aged; D001483:Base Sequence; D015152:Blotting, Northern; D002648:Child; D002675:Child, Preschool; D005260:Female; D006321:Heart / Q000502:physiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D011943:Receptors, Adrenergic, beta / Q000145:classification / Q000235:genetics*
D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta