Cloning, functional expression and tissue distribution of human cDNA for the alpha 1C-adrenergic receptor.
We have cloned human alpha 1C-adrenergic receptor from human prostate cDNA library. The deduced amino acid sequence of the clone (P2C4) encodes a protein of 466 amino acids that showed strong sequence homology to the previously cloned bovine alpha 1C-adrenergic receptor. The radioligand binding properties of P2C4 expressed in COS-7 cells were very similar to those of bovine alpha 1C-adrenergic receptor. With reverse-transcription polymerase chain reaction assay, we observed alpha 1C-adrenergic receptor transcripts in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. The data show that the clone P2C4 encodes a human alpha 1C-adrenergic receptor cDNA, and the receptor subtype is expressed not widely but localized in several human tissues.
Department of Molecular, Cell Pharmacology, National Children's Medical Research Center, Tokyo, Japan.
A
A
Hirasawa
K
K
Horie
T
T
Tanaka
K
K
Takagaki
M
M
Murai
J
J
Yano
G
G
Tsujimoto
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D011869:Radioligand Assay; D011942:Receptors, Adrenergic, alpha / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011942:Receptors, Adrenergic, alpha; D011994:Recombinant Proteins; D004247:DNA