A computer program for the design of PCR primers for diagnosis of highly variable genomes.
PCRDiag (Diagnosis by PCR) is a computer program which allows the localization of pairs of oligonucleotides with optimal thermodynamic requirements for use in a PCR assay. The program is designed for the selection of pairs of primers complementary to sequences present in a group, whose identification is intended, but are absent in other non-specific sequences. The program constitutes a powerful tool, specially in systems which display a high degree of sequence heterogeneity, as is the case of RNA viruses. The program runs on IBM-PC and compatible computers and has no special software requirements. It does not need the previous alignment of the sequences analyzed.
Dopazo
J
J
INIA-Sanidad Animal, Madrid, Spain.
Sobrino
F
F
D000465:Algorithms; D005537:Aphthovirus / Q000235:genetics; D001483:Base Sequence; D017076:Computer-Aided Design; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014644:Genetic Variation; D016678:Genome; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012984:Software; D013816:Thermodynamics
D004277:DNA, Single-Stranded; D004279:DNA, Viral