The expression of transferrin mRNA in the salivary glands and other tissues of baboons.
In order to determine whether all the extrinsic salivary glands synthesize transferrin mRNA, the polyadenylated ribonucleic acids [poly(A)+ RNAs] from parotid, submandibular, and sublingual glands, liver, midbrain, testis, spleen, heart, kidney, and the mucosae of oesophagus and stomach from adult male baboons were analysed, using oligo(dT)-cellulose chromatography, agarose gel electrophoresis, followed by transfer of the mRNAs to nitrocellulose filters and identification with transferrin and tubulin cDNA probes. Transferrin and tubulin mRNAs were visualized by autoradiography and analysed by measuring specific activity from beta emitting nuclides following transfer to nitrocellulose filters and hybridizing with [alpha-32P]-labelled human transferrin and tubulin cDNA probes. The results indicate that transferrin mRNA is present in all the extrinsic salivary glands (submandibular, sublingual, parotid) of baboons.
Winborn
W B
WB
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Weaker
F J
FJ
Sharp
Z D
ZD
Sheridan
P J
PJ
Bowman
B H
BH
Adrian
G S
GS
Yang
F
F
Herbert
D C
DC
D000818:Animals; D015152:Blotting, Northern; D005786:Gene Expression Regulation; D008297:Male; D008636:Mesencephalon / Q000378:metabolism; D009092:Mucous Membrane / Q000378:metabolism; D009928:Organ Specificity; D010215:Papio / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012469:Salivary Glands / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014168:Transferrin / Q000096:biosynthesis* / Q000235:genetics; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics; D014781:Viscera / Q000378:metabolism
D012333:RNA, Messenger; D014168:Transferrin; D014404:Tubulin