[In vitro antimycobacterial activity of a new quinolone, NM394].
We evaluated the in vitro antimicrobial activity of NM394 and ofloxacin (OFLX) against representative pathogenic mycobacteria by the agar dilution method, using 7H11 agar medium. NM394 showed appreciable antimicrobial activity against Mycobacterium tuberculosis (MIC90 = 0.78 micrograms/ml), M. kansasii (MIC90 = 6.25 micrograms/ml), M. marinum (MIC90 = 3.13 micrograms/ml) and M. fortuitum (MIC90 = 3.13 micrograms/ml), whereas the agent was not active against M. scrofulaceum (MIC90 = > 100 micrograms/ml), M. avium (MIC90 = 50 micrograms/ml), M. intracellulare (MIC90 = > 100 micrograms/ml), M. chelonae subsp. abscessus (MIC90 = > 100 micrograms/ml) and M. chelonae subsp. chelonae (MIC90 = 25 micrograms/ml). The in vitro antimicrobial activity of the agent against M. fortuitum was a little more active than that of OFLX, whereas the activity of NM394 against the other mycobacteria was slightly inferior to that of OFLX. The antimycobacterial activity of NM394 against M. tuberculosis H37Rv (MIC:NM394 = 0.78 micrograms/ml, OFLX = 0.78 micrograms/ml) phagocytosed in murine peritoneal macrophages was less active than that of OFLX, when the macrophages were cultured in RPMI-1640 medium containing 1 microgram/ml or 10 micrograms/ml of these agents for up to 5 days.
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
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Tomioka
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Sato
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Saito
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000494:pharmacology*; D005260:Female; D024841:Fluoroquinolones; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects*; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D015242:Ofloxacin / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology*; D015363:Quinolones / Q000494:pharmacology*
D000890:Anti-Infective Agents; D000995:Antitubercular Agents; D024841:Fluoroquinolones; D010879:Piperazines; D015363:Quinolones; D015242:Ofloxacin; C074190:ulifloxacin