Impairment of anti-platelet-aggregating activity of endothelial cells after experimental subarachnoid hemorrhage.
OBJECTIVE
Serial changes of anti-platelet-aggregating activity in the endothelial cells after experimental subarachnoid hemorrhage were studied in 30 feline two-hemorrhage models.


METHODS
One hour or 2, 4, 7, or 14 days after mimic subarachnoid hemorrhage, ADP (40 mg/kg) was infused into the basilar artery via the right vertebral artery to activate circulating platelets. Immediately after ADP infusion, the basilar artery was fixed by intra-arterial perfusion with 1.5% glutaraldehyde in 0.1 mol/L phosphate buffer and was removed. The luminal surface was examined under a scanning electron microscope.


RESULTS
One hour after subarachnoid hemorrhage, no platelets adhered or aggregated on the luminal surface. However, 4 to 7 days after subarachnoid hemorrhage, many platelets were observed adhering or aggregating on the luminal surface.


CONCLUSIONS
These findings suggest the impairment of anti-platelet-aggregating activity of endothelial cells after subarachnoid hemorrhage. This impairment may be involved in inducing cerebral ischemia during cerebral vasospasm by causing platelet adhesion and aggregation.
Ohkuma
H
H
Department of Neurosurgery, Hirosaki University School of Medicine, Japan.
Ogane
K
K
Fujita
S
S
Manabe
H
H
Suzuki
S
S
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D002404:Catheterization; D002415:Cats; D004730:Endothelium, Vascular / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D013345:Subarachnoid Hemorrhage / Q000097:blood* / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors
D000244:Adenosine Diphosphate