Genus-specific detection of salmonellae using the polymerase chain reaction (PCR).
Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.
Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843.
N D
ND
Cohen
H L
HL
Neibergs
E D
ED
McGruder
H W
HW
Whitford
R W
RW
Behle
P M
PM
Ray
B M
BM
Hargis
D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification