Production of interleukin-5 by mouse astrocytes and microglia in culture.
Interleukin (IL)-5 was originally isolated as a B cell growth factor derived from T cells and is known as a multipotent cytokine. We found that IL-5 was detected in the cultures of microglia and astrocytes by both the enzyme-linked immunosolvent assay (ELISA) and bioassay with IL-5-dependent cell line, T88M. We also found that microglia and astrocytes expressed IL-5 mRNA. IL-5 receptor mRNA, however, was not detected in these cells. Stimulation with interferon-gamma increased expression of IL-5 in astrocytes and microglia. These results suggest that IL-5 produced by brain cells may be involved in the interaction between brain cells and immune cells in the brain.
Division of Cell Biology, School of Medicine, Fujita Health University, Aichi, Japan.
M
M
Sawada
A
A
Suzumura
Y
Y
Itoh
T
T
Marunouchi
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D015848:Interleukin-5 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015848:Interleukin-5; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate