Synthetic peptides corresponding to the calmodulin-binding domains of skeletal muscle myosin light chain kinase and human erythrocyte Ca2+ pump interact with and permeabilize liposomes and cell membranes.
Synthetic calmodulin-binding (CaM-binding) peptides (CBPs) representing CaM-binding domains of Ca2+/CaM-dependent enzymes have been reported to interfere with the activity of the melanocyte-stimulating hormone (MSH) receptor function in melanoma cells [Gerst, J. E., & Salomon, Y. (1988) J. Biol. Chem. 263, 7073-7078]. We postulated that membrane lipids may play an important role in the mode of action of CBPs on cells. We therefore tested the ability of CBPs to interact with membrane bilayers. Using artificial phospholipid vesicles, or M2R melanoma cells and cell membranes derived therefrom, as models, we report here that synthetic peptides representing the CaM-binding domains of skeletal muscle myosin light chain kinase (M5) and the human erythrocyte calcium pump (C28W), as well as other CBPs, interact with lipid bilayers and cell membranes. Significant interactions of CBPs with the lipid bilayer were detected in both model systems. M5 and C28W were found to partition into the lipid bilayer of melanoma cell membranes and soybean lecithin vesicles, and surface partition constants obtained (for the liposome model) were in the range 10(3)-10(4) M-1. In addition, C28W and its N-modified NBD derivative were found to inhibit [125I]iodo-[Nle4,D-Phe7]alpha MSH binding to cultured M2R melanoma cells. These and other CBPs were also found to induce the release of cations and calcein from liposomes, suggesting that the interaction of CBPs with the lipid bilayer increases membrane permeability.(ABSTRACT TRUNCATED AT 250 WORDS)
Eshel
Y
Y
Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
Shai
Y
Y
Vorherr
T
T
Carafoli
E
E
Salomon
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D000252:Calcium-Transporting ATPases / Q000097:blood* / Q000502:physiology*; D002147:Calmodulin / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D004912:Erythrocytes / Q000201:enzymology*; D005397:Fish Venoms / Q000494:pharmacology; D005452:Fluoresceins / Q000494:pharmacology; D006801:Humans; D008051:Lipid Bilayers; D008546:Melanoma, Experimental; D008555:Melitten / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D000521:alpha-MSH / Q000378:metabolism
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D005397:Fish Venoms; D005452:Fluoresceins; D008051:Lipid Bilayers; D010455:Peptides; D008555:Melitten; D000521:alpha-MSH; C017603:pardaxin; D009219:Myosin-Light-Chain Kinase; D000252:Calcium-Transporting ATPases; C007740:fluorexon