Molecular analysis of myeloperoxidase deficiency shows heterogeneous patterns of the complete deficiency state manifested at the genomic, mRNA, and protein levels.
Myeloperoxidase (MPO) is a glycosylated hemoprotein contained in the azurophil granules of human polymorphonuclear leukocytes (PMNs). MPO is thought to play a role in the oxidative antimicrobial activity of neutrophils by catalyzing the formation of hypochlorous acid, a potent microbicide, from hydrogen peroxide and chloride anions. Seven unrelated individuals with complete MPO deficiency, a relatively common heritable defect of neutrophils, were identified during routine blood tests. Molecular analyses were conducted to determine the level of the abnormality in these individuals. Western blot analysis showed that 6 of the 7 donors were devoid of immunoreactive MPO protein, while neutrophils from one individual contained only the 55-Kd subunit. Northern analysis of bone marrow RNA from one MPO-deficient donor showed the presence of the normal-sized 3.3-kb transcript indicating that the defect in MPO biosynthesis in this case was posttranscriptional. Southern analysis of four MPO-deficient donors showed a normal Bgl II digestion pattern, whereas an abnormal restriction pattern was observed in a fifth individual. Although the Bgl II pattern was similar to that observed in an unrelated subject described by Nauseef (Blood 73:290, 1989), our study strongly suggests that the point mutation does not reflect a polymorphism. Taken together, these analyses show the existence of diverse abnormalities associated with MPO-deficiency that may be detected at the level of the MPO polypeptide, mRNA, and gene.
Department of Pathology, University of California, Irvine.
M E
ME
Selsted
C W
CW
Miller
M J
MJ
Novotny
W L
WL
Morris
H P
HP
Koeffler
D000328:Adult; D015139:Blotting, Southern; D047209:Eosinophil Peroxidase; D006801:Humans; D009504:Neutrophils / Q000201:enzymology; D009195:Peroxidase / Q000032:analysis / Q000172:deficiency* / Q000235:genetics; D010544:Peroxidases / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D047209:Eosinophil Peroxidase; D010544:Peroxidases; D009195:Peroxidase