Ribonuclease protection assay: use of biotinylated probes for the detection of two messenger RNAs.
A nonradioactive modification of the traditional ribonuclease protection assay is described and applied to the detection of two messenger RNAs. The biotinylated probes are stable and easy to handle, which permits the processing of large numbers of samples. Chemiluminescence is generated with streptavidin-conjugated alkaline phosphatase and provides sensitivity equal to or greater than that achieved with the radioactive detection method.
Department of Biochemistry and Molecular Biology, Texas Tech University, Health Sciences Center, Lubbock 79430.
M A
MA
Turnbow
C W
CW
Garner
D000199:Actins / Q000235:genetics; D000818:Animals; D001710:Biotin; D055504:Insulin Receptor Substrate Proteins; D051379:Mice; D010750:Phosphoproteins / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D012260:Ribonucleases / Q000494:pharmacology*
D000199:Actins; D055504:Insulin Receptor Substrate Proteins; C526295:Irs1 protein, mouse; D010750:Phosphoproteins; D015347:RNA Probes; D012333:RNA, Messenger; D001710:Biotin; D012260:Ribonucleases