cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for kappa-subtype-selective ligands.
The amino acid sequence of a rat opioid receptor, designated as ROR-D, has been deduced by cloning and sequencing the cDNA. The ROR-D expressed from the cDNA exhibits high affinities for ligands selective for the opioid receptor kappa-subtype and low affinities for ligands selective for the delta- and mu-subtypes. RNA blot hybridization analysis indicated that ROR-D mRNA is distributed in cerebrum and brainstem but not in cerebellum.
International Institute for Advanced Studies, Kyoto, Japan.
M
M
Nishi
H
H
Takeshima
K
K
Fukuda
S
S
Kato
K
K
Mori
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA; D008024:Ligands; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D017465:Receptors, Opioid, delta / Q000187:drug effects / Q000378:metabolism; D017473:Receptors, Opioid, kappa / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000187:drug effects / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D008024:Ligands; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D004247:DNA