Expression of mouse furin in a Chinese hamster cell resistant to Pseudomonas exotoxin A and viruses complements the genetic lesion.
RPE.40 is a strain of mutated CHO-K1 cells with elevated resistance to Pseudomonas exotoxin A, Sindbis virus, and Newcastle disease virus. Virus resistance is due to an inability to cleave precursor viral membrane glycoproteins and produce infectious virions. Transfection of RPE.40 cells with cDNA for mouse furin causes them to lose all resistance and become as sensitive as wild-type cells to the toxin and viruses. Transfection of RPE.40 cells with cDNA for the related yeast protease Kex2 reduces their resistance to the toxin and viruses, but does not completely eliminate it.
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405.
J M
JM
Moehring
N M
NM
Inocencio
B J
BJ
Robertson
T J
TJ
Moehring
D036002:ADP Ribose Transferases; D000818:Animals; D001427:Bacterial Toxins; D016466:CHO Cells; D006224:Cricetinae; D004352:Drug Resistance, Microbial; D005098:Exotoxins / Q000494:pharmacology*; D045683:Furin; D005816:Genetic Complementation Test; D009522:Newcastle disease virus / Q000254:growth & development; D043484:Proprotein Convertases; D014176:Protein Biosynthesis; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases / Q000378:metabolism; D013539:Simian virus 40 / Q000254:growth & development; D013381:Subtilisins / Q000502:physiology*; D014162:Transfection; D014762:Viral Interference; D014764:Viral Proteins / Q000096:biosynthesis; D037521:Virulence Factors; D014779:Virus Replication; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D045683:Furin; D043484:Proprotein Convertases; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D013381:Subtilisins; D014764:Viral Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C058751:KEX2 protein, S cerevisiae