FAD-linked glycerophosphate dehydrogenase activity in islets, liver, and splenocytes of NOD mice.
The activity of FAD-linked glycerophosphate dehydrogenase (m-GDH), as well as that of glutamate dehydrogenase and both glutamate-oxalacetate and glutamate-pyruvate transaminases, were measured in islet, liver, and splenocyte homogenates from 6- to 7-week-old female nonobese diabetic mice (NOD) and age- and sex-matched control mice. Despite incipient insulitis and euglycemia, the NOD mice displayed both high islet insulin content and elevated insulinemia. The activity of m-GDH, expressed relative to protein content, was not decreased in islets of NOD mice, despite the fact that such a specific activity is lower in splenic lymphocytes than islet cells. In liver homogenates, the activity of m-GDH was even higher in NOD than control mice. It is proposed, therefore, that in this model of insulin-dependent diabetes no primary decrease in islet m-GDH activity occurs, at variance with the situation recently documented in several animal models of non-insulin-dependent diabetes.
Anak
O
O
Laboratory of Experimental Medicine, Erasmus Medical School, Brussels Free University, Belgium.
Malaisse-Lagae
F
F
Leclercq-Meyer
V
V
Sener
A
A
Malaisse
W J
WJ
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D007118:Immunoassay; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000201:enzymology* / Q000473:pathology; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D013154:Spleen / Q000201:enzymology* / Q000473:pathology
D007328:Insulin; D005182:Flavin-Adenine Dinucleotide; D005993:Glycerolphosphate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase