Reconstitution of a purified mu-opioid binding protein in liposomes: selective, high affinity, GTP gamma S-sensitive mu-opioid agonist binding is restored.
An opioid binding protein (OBP) purified to homogeneity from bovine striatal membranes has been reconstituted in liposomes. The liposomes were produced by PEG-precipitation of OBP in the presence of a CHAPS extract of bovine striatum, devoid of opioid binding. High affinity mu-agonist binding was restored. The binding was selective for mu-agonists, stereospecific and inhibited by GTP gamma S. These results demonstrate that there is recoupling of OBP with G-protein and confirm our earlier evidence that the purified OBP is a mu-opioid binding site.
Gioannini
T L
TL
Natural Sciences Department, Baruch College, New York, NY 10016.
Fan
L Q
LQ
Hyde
L
L
Ofri
D
D
Yao
Y H
YH
Hiller
J M
JM
Simon
E J
EJ
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002793:Cholic Acids; D003342:Corpus Striatum / Q000378:metabolism*; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D007700:Kinetics; D008081:Liposomes; D009020:Morphine / Q000378:metabolism*; D009270:Naloxone / Q000378:metabolism*; D009294:Narcotics / Q000494:pharmacology; D017450:Receptors, Opioid, mu / Q000302:isolation & purification / Q000378:metabolism*
D002793:Cholic Acids; D004745:Enkephalins; D008081:Liposomes; D009294:Narcotics; D017450:Receptors, Opioid, mu; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D009270:Naloxone; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D009020:Morphine; D020881:Enkephalin, D-Penicillamine (2,5)-; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate