Simultaneous determination of xylazine and its major metabolite, 2,6-dimethylaniline, in bovine and swine kidney by liquid chromatography.
A liquid chromatographic (LC) method is described for the simultaneous determination of xylazine (XY) and its major metabolite, 2,6-dimethylaniline (2,6-DMA), in bovine and swine kidney in the 25-100 ppb range. XY and 2,6-DMA are extracted from kidney with chloroform, followed by cleanup on an acidic Celite 545 column. A mu Bondapak phenyl column is used for LC separation with UV determination at 225 nm. The mobile phase is a mixture of acetonitrile, water, sodium acetate, and acetic acid. Mean recoveries from bovine kidney were 78.3% for XY, with a standard deviation (SD) of 7.45 and a coefficient of variation (CV) of 9.51%, and 87.2% for 2,6-DMA, with an SD of 8.38 and a CV of 9.61%. Mean recoveries from swine kidney were 80.8% for XY, with an SD of 5.92 and a CV of 7.33%, and 86.7% for 2,6-DMA, with an SD of 6.16 and a CV of 7.10%.
Holland
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U.S. Food and Drug Administration, Denver Federal Center, CO 80225-0087.
Munns
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Roybal
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Hurlbut
J A
JA
Long
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D000814:Aniline Compounds / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014991:Xylazine / Q000032:analysis*
D000814:Aniline Compounds; D007202:Indicators and Reagents; D012997:Solvents; D014991:Xylazine; C007766:2,6-xylidine