Indirect evidence for two distinct mechanisms of DNA cleavage at the oriT of RP4 during conjugation.
Mobilization of a potential Campylobacter shuttle vector from Escherichia coli K-12 into C. hyointestinalis generated a small plasmid harboring a hybrid oriT nick region. This is presumed to have resulted from the cleavage and religation of the oriT of RP4 and the putative nick region of the shuttle vector. These data imply that two distinct DNA cleavage reactions occur at the oriT site of RP4 during conjugal transfer.
Department of Microbiology and Immunology, University of Adelaide, Australia.
S R
SR
Waterman
J
J
Hackett
P A
PA
Manning
D001483:Base Sequence; D002167:Campylobacter / Q000235:genetics*; D003227:Conjugation, Genetic; D003360:Cosmids / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011815:R Factors / Q000235:genetics*
D004269:DNA, Bacterial