Antigen processing mutant T2 cells present viral antigen restricted through H-2Kb.
Cytotoxic T lymphocytes (CTL) recognize foreign antigens as short peptides presented by class I molecules of the major histocompatibility complex (MHC). T2 cells are profoundly defective in the presentation of endogenously synthesized antigens to CTL due to a deletion of MHC class II-encoded genes for transporters associated with antigen presentation (TAP1/TAP2). Surprisingly, we here demonstrate that T2 cells, after infection with Sendai virus, are readily killed by H-2Kb restricted CD8+ T cells. In contrast to classical class I-mediated antigen presentation, the presentation of Sendai virus antigen in T2Kb cells is brefeldin A (BFA) insensitive. The present findings may suggest the presence of an alternative pathway for MHC class I-mediated antigen presentation in T2 cells.
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
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X
Zhou
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R
Glas
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T
Liu
H G
HG
Ljunggren
M
M
Jondal
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D000956:Antigens, Viral / Q000276:immunology*; D020126:Brefeldin A; D002460:Cell Line; D003517:Cyclopentanes / Q000494:pharmacology; D005260:Female; D006183:H-2 Antigens / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009975:Orthomyxoviridae / Q000276:immunology; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
D000956:Antigens, Viral; D003517:Cyclopentanes; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D020126:Brefeldin A