Study of ischemic myocardial buffering capacity in perfused rat heart through rapidly interleaved 1H and 31P MRS measurements.
We present a method for the direct correlation of quantitative changes in lactate concentration and intracellular pH during global ischemia in the perfused rat heart using rapidly interleaved 31P MRS and water-suppressed 1H MRS acquisitions. The ischemic myocardial buffering capacity was determined and found to be consistent with previous experimentation.
Department of Biochemistry, University of Oxford, United Kingdom.
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Schussheim
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GK
Radda
D000818:Animals; D002021:Buffers; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000032:analysis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010758:Phosphorus; D051381:Rats; D011918:Rats, Inbred SHR
D002021:Buffers; D007773:Lactates; D010758:Phosphorus; D006859:Hydrogen