[Expression of pel genes of Erwinia chrysanthemi ENA49 in Erwinia carotovora var. atroseptica 36A cells].
Erwinia atroseptica 36A cells were transformed by the recombinant plasmid pPL5-1 (a derivative of the vector plasmid pUC19) containing pelb and pelc genes which encode pectate lyases of Erwinia chrysanthemi ENA49. Synthesis of pectate lyases PLB and PLC determined by the cloned pel genes is constitutive in Erwinia atroseptica 36ApPL5-1 cells and not inducible by sodium polypectate. The major part of these enzymes was accumulated in the periplasmic fraction of Erwinia atroseptica and cells were unable to efficiently secrete the enzymes into the cultural medium. Synthesis and secretion of the native pectate lyases by Erwinia atroseptica harboring the plasmid were as efficient as by the parental cells. The obtained results suggest the high specificity of pectate lyase secretory systems of kindred Erwinias.
Babitskaia
E V
EV
Evtushenkov
A N
AN
Shevchik
V E
VE
Zheldakova
R A
RA
D003001:Cloning, Molecular; D016974:Dickeya chrysanthemi / Q000235:genetics*; D005798:Genes, Bacterial; D016973:Pectobacterium carotovorum / Q000235:genetics*; D010957:Plasmids; D011133:Polysaccharide-Lyases / Q000235:genetics*
D011133:Polysaccharide-Lyases; C023911:pectate lyase