Nucleotide sequence of class D tetracycline resistance genes from Salmonella ordonez.
Plasmid pIP173, isolated from Salmonella ordonez strain BM2000, confers resistance to tetracycline and a number of other antibiotics. We determined the nucleotide sequence of the pIP173 tetR repressor and tetA resistance genes. The pIP173 tetR gene is essentially identical to the class D tetR gene from plasmid RA1. The pIP173 tet genes are flanked by directly repeated copies of the insertion sequence IS26. Interestingly, the 3' end of the tetR gene, encoding the C-terminal 16 amino acids of the TetR protein, extends into the flanking IS26 sequence. The relationships between the class A, B, C, and D TetA sequences parallel the relationships between the corresponding TetR sequences; class D is more closely related to class B than to either class A or C. Overall, the four TetA sequences show 38% identity and 57% similarity.
Allard
J D
JD
Department of Microbiology, Washington State University, Pullman 99164.
Gibson
M L
ML
Vu
L H
LH
Nguyen
T T
TT
Bertrand
K P
KP
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004251:DNA Transposable Elements / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011815:R Factors / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D012475:Salmonella / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013753:Tetracycline Resistance / Q000235:genetics*
D004251:DNA Transposable Elements; D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein