Effect of interleukin-6 on cell proliferation of FRTL-5 cells.
We studied the effects of interleukin-6(IL-6) on DNA synthesis and cyclic AMP production in rat thyroid FRTL-5 cells. When cells were incubated with IL-6 in the presence or absence of IGF-I, cell proliferation was not observed. By contrast, IL-6 stimulated DNA synthesis in a dose dependent manner when TSH was added concomitantly. On the other hand, IL-6 did not modulate the cAMP accumulation in the presence or absence of TSH. These data demonstrate that, like IGF-I, IL-6 may be able to act as a growth factor through activation of a mitogenic signal transduction pathway different from A-kinase in FRTL-5 cells.
Department of Laboratory Medicine, Osaka University School of Medicine, Japan.
S
S
Nishiyama
K
K
Takada
H
H
Tada
T
T
Takano
N
N
Amino
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D015850:Interleukin-6 / Q000502:physiology*; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology* / Q000378:metabolism
D015850:Interleukin-6; D004247:DNA; D000242:Cyclic AMP