Wheat germ agglutinin-induced intracellular calcium mobilization in human platelets: suppression by staurosporine and resistance to cyclic AMP inhibition.
The lectin wheat germ agglutinin (WGA) elicited a prompt and sharp increase in intracellular Ca2+ concentration in human platelets. The WGA-induced Ca2+ mobilization was markedly inhibited by a protein kinase inhibitor staurosporine, whereas Ca2+ mobilization by receptor-mediated agonists, including thrombin, platelet-activating factor, and arginine-vasopressin, was not. In contrast, the lectin-induced Ca2+ mobilization was resistant to cyclic AMP inhibition, compared with that induced by receptor-mediated agonists. These findings indicate that the mechanism of intracellular Ca2+ mobilization, or possibly phospholipase C activation, induced by WGA is different from that induced by receptor-mediated agonists in human platelets.
Department of Laboratory Medicine, Yamanashi Medical College, Japan.
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Yatomi
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Ozaki
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Y
Koike
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Satoh
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Kume
D000470:Alkaloids / Q000494:pharmacology*; D000527:Alprostadil / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology*; D004789:Enzyme Activation; D006801:Humans; D066298:In Vitro Techniques; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D019311:Staurosporine; D013917:Thrombin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism; D014909:Wheat Germ Agglutinins / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D000470:Alkaloids; D014909:Wheat Germ Agglutinins; D003994:Bucladesine; D000242:Cyclic AMP; D011493:Protein Kinase C; D010738:Type C Phospholipases; D013917:Thrombin; D000527:Alprostadil; D019311:Staurosporine; D002118:Calcium