Identification of a DNA-binding protein of human herpesvirus 6, a putative DNA polymerase stimulatory factor.
A 41K early nuclear antigen (p41), expressed in human herpesvirus type 6 (HHV-6)-infected T cells, was cloned by screening a cDNA expression library with the anti-p41 monoclonal antibody (MAb) C5. When expressed in mammalian cells, the cloned p41 protein comigrated with the authentic p41 protein from HHV-6-infected cells and localized to the nucleus. HHV-6 p41 shares 44% sequence identity with the human cytomegalovirus (HCMV) DNA-binding protein, ICP36 (UL44 gene product); p41 binds to ssDNA with the same apparent affinity as ICP36. Since ICP36 has recently been shown to be an HCMV DNA polymerase-associated stimulatory factor, a similar function is suggested for p41.
Agulnick
A D
AD
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104-4268.
Thompson
J R
JR
Iyengar
S
S
Pearson
G
G
Ablashi
D
D
Ricciardi
R P
RP
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; C072217:ICP36 protein, Cytomegalovirus; D012333:RNA, Messenger; D014764:Viral Proteins; C081690:p41 protein, Human herpesvirus 6; D004259:DNA-Directed DNA Polymerase