Distribution of beta 2-adrenergic receptor mRNA expression along the hamster nephron segments.
Distribution of beta 2-adrenergic receptor mRNA expression along the microdissected hamster nephron segments was examined by the reverse transcription-polymerase chain reaction (RT-PCR) technique. Conventional RT-PCR using a set of primers on separate exons could not be applied for the detection of beta 2-adrenergic receptor mRNA because of its intronless nature. We used the 'rapid amplification of cDNA ends' protocol [(1985) Proc. Natl. Acad. Sci. USA 85, 8998-9002] as a maneuver for RT-PCR of an intronless gene. Using this method, we successfully located hamster beta 2-adrenergic receptor mRNA only in glomeruli and early proximal convoluted tubule along the nephron segments tested.
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
S
S
Taniguchi
T
T
Watanabe
A
A
Nakao
G
G
Seki
S
S
Uwatoko
K
K
Suzuki
K
K
Kurokawa
D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D004247:DNA; D005260:Female; D007438:Introns; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009399:Nephrons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000235:genetics*
D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D004247:DNA