Hormonal control of atrial natriuretic peptide synthesis and secretion from cultured atrial myocytes.
We have established optimal culture conditions for growing well-differentiated atrial myocytes from newborn rats. These myocytes show the morphological features of myocytes in intact atria; including perinuclear endocrine storage granules, contractile apparatus and numerous mitochondria. The cells synthesise, store and secrete immunoreactive ANP. Stored ANP is of similar molecular weight to pro-ANP (1-126); secreted ANP is the active form ANP (99-126) but processing appears to take place slowly after secretion. ANP secretion is stimulated by endothelin. Synthesis and secretion of ANP are markedly reduced by the beta-adrenergic agonist isoproterenol, and by forskolin.
Muir
T M
TM
Department of Biochemistry, University of Glasgow, Scotland.
Hair
J
J
Inglis
G C
GC
Dow
J W
JW
Lindop
G B
GB
Leckie
B J
BJ
D000818:Animals; D016275:Atrial Function; D009320:Atrial Natriuretic Factor / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D001790:Blood Physiological Phenomena; D002454:Cell Differentiation / Q000502:physiology; D002457:Cell Extracts; D002478:Cells, Cultured; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology; D006325:Heart Atria / Q000187:drug effects / Q000378:metabolism*; D006728:Hormones / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D008854:Microscopy, Electron; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D002457:Cell Extracts; D003470:Culture Media; D006728:Hormones; D003907:Dexamethasone; D009320:Atrial Natriuretic Factor; D007545:Isoproterenol