Column liquid chromatographic analysis of quinine in human plasma, saliva and urine.
A new simple, selective and reproducible high-performance liquid chromatographic method for the determination of quinine in plasma, saliva and urine is described. The ion-pair method was carried out on a reversed-phase C18 column, using perchlorate ion as the counter ion and ultraviolet detection at 254 nm. Quinine was well resolved from its major metabolite, 3-hydroxyquinine, and the internal standard, primaquine. The limit of detection was 10 ng/ml and the recovery was greater than 90% from the three biological fluids.
Babalola
C P
CP
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
Bolaji
O O
OO
Dixon
P A
PA
Ogunbona
F A
FA
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D011319:Primaquine / Q000032:analysis / Q000097:blood / Q000652:urine; D011802:Quinidine / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood / Q000652:urine; D011803:Quinine / Q000032:analysis* / Q000097:blood / Q000652:urine; D012463:Saliva / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; C013569:3-hydroxyquinidine; D011803:Quinine; D011802:Quinidine; D011319:Primaquine