SGP2, ubiquitin, 14K lectin and RP8 mRNAs are not induced in neuronal apoptosis.
Cultured embryonic sympathetic neurones and differentiated PC12 cells undergo apoptosis when deprived of nerve growth factor (NGF). Apoptosis caused by NGF deprivation can be prevented by inhibitors of RNA and protein synthesis suggesting the involvement of newly synthesized gene products in the death process. We have examined in these neurones, the expression of four genes known to be stimulated in non-neuronal cells, specifically during apoptosis. The levels of SGP-2, ubiquitin, and RP-8 mRNAs are not altered during neuronal death. Expression of 14-K lectin mRNA is down-regulated 3-4 fold. These results show that the four genes examined do not serve as 'death genes' in the induction of apoptosis in neuronal cells and raise the possibility that other genes and mechanisms are involved.
D'Mello
S R
SR
Institute of Neurobiology, CNR, Rome, Italy.
Galli
C
C
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D002478:Cells, Cultured; D051152:Clusterin; D015536:Down-Regulation / Q000502:physiology; D006023:Glycoproteins / Q000235:genetics; D037102:Lectins / Q000235:genetics; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000378:metabolism*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000096:biosynthesis*; D013564:Sympathetic Nervous System / Q000166:cytology / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics
D051152:Clusterin; D006023:Glycoproteins; D037102:Lectins; D018832:Molecular Chaperones; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014452:Ubiquitins