Human erythrocyte sialidase is linked to the plasma membrane by a glycosylphosphatidylinositol anchor and partly located on the outer surface.
Treatment of human erythrocyte ghosts with phosphatidylinositol-phospholipase C (PIPLC) from Bacillus cereus liberated the ghost-linked sialidase. Maximal release of sialidase (about 70% of total) was achieved by incubating ghosts at 37 degrees C for 60 min, at pH 6.0, with PIPLC (PIPLC total units/ghost protein ratio, 4.5 each time) added at the beginning of incubation and every 15 min (four subsequent additions). Liberated sialidase was fully resistant to at least four cycles of rapid freezing and thawing and to storage at 4 degrees C for at least 48 h. The liberated enzyme had an optimal activity at pH 4.2, degraded ganglioside GD1a better than methylumbelliferyl N-acetylneuraminic acid (about fourfold), and gave a Km value of 2.56 x 10(-4) M and an apparent Vmax of 2.22 mU per mg protein on GD1a. Treatment of intact erythrocytes with PIPLC (PIPLC total units/erythrocyte protein ratio, 8), under conditions where haemolysis was practically negligible, caused liberation of 10-12% of membrane linked sialidase, indicating that the enzyme is, at least in part, located on the outer surface of the erythrocyte membrane. It is concluded that the erythrocyte membrane sialidase is anchored by a glycosylphosphatidylinositol structure sensitive to PIPLC action, and is partly located on the outer surface.
Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.
A
A
Chiarini
A
A
Fiorilli
L
L
Di Francesco
B
B
Venerando
G
G
Tettamanti
D004910:Erythrocyte Membrane / Q000201:enzymology*; D017261:Glycosylphosphatidylinositols / Q000737:chemistry*; D006801:Humans; D009439:Neuraminidase / Q000097:blood*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010727:Phosphoric Diester Hydrolases
D017261:Glycosylphosphatidylinositols; D010727:Phosphoric Diester Hydrolases; D009439:Neuraminidase; D043265:Phosphatidylinositol Diacylglycerol-Lyase