Induction of intercalated cell changes in rat pups from acid- and alkali-loaded mothers.
We evaluated the effects of acid-base changes in pregnant and lactating dams on intercalated cell morphology and populations in newborn and 2-wk-old rats. Collecting ducts were studied with transmission electron microscopy with intercalated cells identified by the presence of 10-nm studs in the cytoplasmic face of apical membranes (A-type intercalated cells) or basolateral membranes (B-type intercalated cells). In newborn and 2-wk-old pups from dams with metabolic alkalosis, there was a significantly larger percentage of B-type intercalated cells and a smaller percentage of A-type intercalated cells. Acid loading with NH4Cl, however, did not produce an increase in the percentage of A-type intercalated cells, but reduced the percentage of B-type intercalated cells. We conclude that maternal metabolic alkalosis is associated with an increase in the percentage of B-type intercalated cells, suggesting that the initial differentiation of intercalated cells is responsive to maternal acid-base disturbances.
Department of Anatomy, University of Ottawa, Ontario, Canada.
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Narbaitz
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Kapal
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Levine
D000137:Acid-Base Imbalance / Q000139:chemically induced / Q000473:pathology*; D000471:Alkalosis / Q000139:chemically induced / Q000473:pathology*; D000643:Ammonium Chloride; D000818:Animals; D000831:Animals, Newborn; D001639:Bicarbonates; D002452:Cell Count; D005260:Female; D007685:Kidney Tubules, Collecting / Q000648:ultrastructure*; D007774:Lactation; D008854:Microscopy, Electron; D011247:Pregnancy; D011248:Pregnancy Complications; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium; D017693:Sodium Bicarbonate
D001639:Bicarbonates; D000643:Ammonium Chloride; D017693:Sodium Bicarbonate; D012964:Sodium