Marker rescue of a transformation-negative Epstein-Barr virus recombinant from an infected Burkitt lymphoma cell line: a method useful for analysis of genes essential for transformation.
A Burkitt lymphoma cell line infected in vitro with a transformation-defective mutant recombinant Epstein-Barr virus (EBV) was used to attempt marker rescue of transformation competence by transfection with cloned wild-type DNA. EBV replication was induced in the transfected cells, and wild-type EBV DNA recombined via flanking homologous sequences adjacent to the deletion, resulting in a virus which transformed primary B lymphocytes in vitro. This strategy should be useful for molecular genetic analysis of the role of part or all of any gene in cell growth transformation.
Marchini
A
A
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115.
Kieff
E
E
Longnecker
R
R
D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000382:microbiology; D002051:Burkitt Lymphoma / Q000382:microbiology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D015870:Gene Expression; D005819:Genetic Markers; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D014162:Transfection / Q000235:genetics; D014407:Tumor Cells, Cultured
D005819:Genetic Markers