Effect of hypoxia on the accumulation of technetium-99m-glucarate and technetium-99m-gluconate by Chinese hamster ovary cells in vitro.
The accumulation of 99mTc-glucarate, an agent recently reported to localize in acutely infarcted myocardium, zones of acute cerebral injury and tumors, has been compared with 99mTc-gluconate in an in vitro system of cultured Chinese hamster ovary fibroblasts. The effects on accumulation of hypoxia and competition with fructose have been studied. Both labeled glucose analogs showed a two- to threefold enhanced accumulation in hypoxic cells relative to aerobic cells. No such enhanced accumulation under hypoxia was observed for the nonsugar tracers pertechnetate and 99mTc-DTPA. The presence of 20 mM fructose reduced the accumulation of 99mTc-glucarate by 30% (p = 0.067) and 99mTc-gluconate by 40% (p < 0.05) in hypoxic cells, but had no significant effect in aerobic cells. These results suggest that both compounds at least partially share a common mechanism of uptake and/or accumulation with fructose.
Division of Nuclear Medicine, Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Canada.
J R
JR
Ballinger
D S
DS
Cowan
I
I
Boxen
Z M
ZM
Zhang
A M
AM
Rauth
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005632:Fructose / Q000494:pharmacology; D005937:Glucaric Acid / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005942:Gluconates / Q000493:pharmacokinetics*; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D010053:Ovary; D013670:Sodium Pertechnetate Tc 99m / Q000493:pharmacokinetics; D016284:Technetium Tc 99m Pentetate / Q000493:pharmacokinetics
D005942:Gluconates; D015609:Organotechnetium Compounds; C067171:technetium Tc 99m glucarate; C032080:technetium Tc 99m gluconate; D005632:Fructose; D013670:Sodium Pertechnetate Tc 99m; D005937:Glucaric Acid; D016284:Technetium Tc 99m Pentetate