Characterization of a full-length cDNA encoding human liver S-adenosylmethionine synthetase: tissue-specific gene expression and mRNA levels in hepatopathies.
The sequence of a full-length cDNA coding for human liver S-adenosylmethionine synthetase has been determined. It spans 3217 nucleotides and encodes a protein of 395 amino acid residues, with a calculated molecular mass of 43,647 Da. The structural features deduced from the amino acid sequence show a close similarity to those of the rat liver enzyme. The liver-specific S-adenosylmethionine synthetase gene appears to be present as a single copy in the genome, as revealed by Southern analysis. The occurrence of a single mRNA species for this enzyme has been determined by primer extension and Northern analysis. Among several human tissues examined, this gene is expressed only in the liver. Similar S-adenosylmethionine synthetase mRNA levels have been detected in biopsies from normal human liver and from patients with alcoholic cirrhosis and hepatocellular carcinoma. Based on these results, a possible mechanism of regulation of human liver S-adenosylmethionine synthetase is discussed.
Instituto de Investigaciones Biomédicas, C.S.I.C., Calle Arturo Duperier 4, Madrid, Spain.
L
L
Alvarez
F
F
Corrales
A
A
Martín-Duce
J M
JM
Mato
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D004247:DNA; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008104:Liver Cirrhosis, Alcoholic / Q000201:enzymology*; D008113:Liver Neoplasms / Q000201:enzymology*; D008716:Methionine Adenosyltransferase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D012333:RNA, Messenger; D004247:DNA; D008716:Methionine Adenosyltransferase