A rapid optimized protocol for downward alkaline Southern blotting of DNA.
A simple and efficient 2.5-h Southern blotting procedure is described that uses 0.4 M NaOH to transfer DNA in a downward direction. The resulting blots give signals that are both sharper and 30% stronger than those obtained by conventional upward-transferred blots.
Koetsier
P A
PA
Institute for Genetics, University of Cologne, FRG.
Schorr
J
J
Doerfler
W
W
D000818:Animals; D015139:Blotting, Southern / Q000379:methods*; D004247:DNA / Q000032:analysis*; D051379:Mice; D012972:Sodium Hydroxide
D012972:Sodium Hydroxide; D004247:DNA