Phenotypic and functional characterization of mouse attenuated and virulent variants of foot-and-mouth disease virus type O1 Campos.
A series of genetically related variants arising from a parental wild-type isolate of O1 Campos and its tissue culture adapted variant were differentiated by various cell culture markers (temperature sensitivity, plaque size, viral yield) and lethality in mice. These isolates were additionally characterized functionally and biochemically by examining poly(C) length, RNA synthesis, protein synthesis, and cell receptor binding. In primary bovine kidney cells, the virulent isolates had greater levels of protein synthesis, whereas in baby hamster kidney cells, the attenuated variant outproduced the wild-type parent. The tissue culture adapted variant had substantially greater ability to attach to cells than the parental wild type. The parental wild-type and the tissue culture-adapted variant were similarly neutralized by various sera against whole virus, but the parental wild type was less effectively neutralized by sera prepared from either full or truncated (variable region) bacterially expressed VP1 polypeptides. The capsid region of the genomes of both these variants was sequenced and a nucleotide substitution resulting in a change in amino acid 56 in VP3 was found. The nucleotide sequence change for the remaining two variants was that of the parental wild-type virus.
Molecular Biology Laboratory, Plum Island Animal Disease Center, USDA-Agricultural Research Service, Greenport, New York 11944.
M J
MJ
Jensen
D M
DM
Moore
D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D014644:Genetic Variation; D007668:Kidney; D007700:Kinetics; D008297:Male; D008715:Methionine / Q000378:metabolism; D051379:Mice; D009500:Neutralization Tests; D010641:Phenotype; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D011991:Receptors, Virus / Q000502:physiology; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis; D014774:Virulence
D012367:RNA, Viral; D011991:Receptors, Virus; D014764:Viral Proteins; D008715:Methionine