Phospholipase C activity in cerebrospinal fluid following subarachnoid hemorrhage related to brain damage.
Phosphoinositide-specific phospholipase C (PLC) activities were measured in CSF from patients after subarachnoid hemorrhage (SAH). Their PLC activities were significantly higher than those in control CSF. Moreover, there was an obvious correlation between the PLC activity in CSF collected on day 3 and the preoperative clinical grade. The PLC activity was also closely correlated with the level of neuron-specific enolase as a marker of brain damage. Furthermore, the PLC activities were partially purified from CSF of patients after SAH and were immunologically identified to be PLC beta, PLC gamma, and PLC delta. These results suggest that PLCs are released into the CSF from brain tissue in conjunction with the initial hemorrhage and that their activity may reflect the extent of brain damage.
Department of Neurosurgery, Gifu University School of Medicine, Japan.
T
T
Nakashima
K
K
Takenaka
Y
Y
Nishimura
T
T
Andoh
N
N
Sakai
H
H
Yamada
Y
Y
Banno
Y
Y
Okano
Y
Y
Nozawa
D017542:Aneurysm, Ruptured / Q000134:cerebrospinal fluid / Q000201:enzymology; D006801:Humans; D002532:Intracranial Aneurysm / Q000134:cerebrospinal fluid / Q000201:enzymology; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010751:Phosphopyruvate Hydratase / Q000134:cerebrospinal fluid; D010727:Phosphoric Diester Hydrolases / Q000134:cerebrospinal fluid* / Q000302:isolation & purification; D013345:Subarachnoid Hemorrhage / Q000134:cerebrospinal fluid / Q000201:enzymology*; D010738:Type C Phospholipases / Q000134:cerebrospinal fluid*
D010727:Phosphoric Diester Hydrolases; D010738:Type C Phospholipases; D010751:Phosphopyruvate Hydratase; D043265:Phosphatidylinositol Diacylglycerol-Lyase