Suppression of taurine response in acutely dissociated substantia nigra neurons by intracellular cyclic AMP.
The modulatory effect of intracellular cyclic AMP on the taurine response was investigated in acutely dissociated rat substantia nigra neurons in patch clamp configurations. Taurine acts mainly on the glycine receptor. An intracellular application of cyclic AMP (5 x 10(-4) M) inhibited the response to a high concentration of taurine (10(-3) M) by about 50%, but did not affect the response to a low concentration of taurine (10(-4) M). This inhibition was blocked somewhat by N-(2-[methylamino]ethyl)-5-isoquinolinesulfonamide (H-8) (10(-6) M), suggesting that the inhibition of taurine response might be partly mediated by an activation of protein kinase A.
College of Allied Medical Science, Akita University, Japan.
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Inomata
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Nabekura
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Akaike
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D004594:Electrophysiology; D007425:Intracellular Membranes / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013654:Taurine / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D013654:Taurine; D000242:Cyclic AMP