Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification.
Genomic RNA fingerprints of infectious bronchitis virus (IBV) strains M41 and Conn46 were prepared to identify T1 RNase-resistant oligonucleotides 'unique' to each of the two IBV strains. Such oligonucleotides were subsequently eluted from the gels and their nucleotide sequences determined. When oligonucleotide probes of those sequences were synthesized and used in a dot-blot hybridization assay, the probes lacked IBV strain-specificity and reacted with the RNAs of homologous as well as heterologous IBV strains. Based on these results, the methods used in this study need to be applied to a large number of oligonucleotide probes, to find one or a few that might be suitable as IBV strain- or serotype-specific oligonucleotide probes.
Department of Avian and Aquatic Animal Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY.
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Karaca
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Palukaitis
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Naqi
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D003333:Coronaviridae Infections / Q000175:diagnosis*; D001351:Infectious bronchitis virus / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D015345:Oligonucleotide Probes; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006163:Ribonuclease T1 / Q000378:metabolism; D017423:Sequence Analysis, RNA
D015345:Oligonucleotide Probes; D012367:RNA, Viral; D006163:Ribonuclease T1