Production of multiple growth factors by a human non-small cell lung carcinoma cell line.
The 1PT cell line, derived from an undifferentiated bronchial carcinoma, produced, in conditioned medium, immunoreactive basic fibroblast growth factor (bFGF), insulin-like growth factors I and II (IGF-I and IGF-II), epidermal growth factor (EGF), transforming growth factor alpha (TGF alpha), and transforming growth factor beta-2 (TGF beta 2) in its latent form, but not platelet-derived growth factor (PDGF), tumour necrosis factor alpha (TNF alpha), or transforming growth factor beta-1 (TGF beta 1). Comparative studies of growth stimulation of human umbilical vein (HUV) endothelial cells indicated that the growth factors detected in 1PT-conditioned medium do not solely account for its proliferative effects on these cells. These results support previous characterization studies [1,2] that suggest the production of a potentially novel tumour-derived endothelial cell growth factor by the 1PT cell line.
Clatterbridge Cancer Research Trust, J.K. Douglas Cancer Research Laboratories, Clatterbridge Hospital, Bebington, Wirral, UK.
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Occleston
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Walker
D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D002460:Cell Line / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000737:chemistry; D016228:Endothelial Growth Factors / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*
D017077:Culture Media, Conditioned; D016228:Endothelial Growth Factors; D006133:Growth Substances