Intravenous infusion of tridocosahexaenoyl-glycerol emulsion into rabbits. Effects on leukotriene B4/5 production and fatty acid composition of plasma and leukocytes.
Leukotriene (LT) B4 is a major chemical activator of PMN. Inhibitory effects of oral administration of docosahexaenoic acid (DHA) on LTB4 synthesis by PMN are known. We intravenously infused tridocosahexaenoyl-glycerol (DHA-TG) emulsion into rabbits in three different doses, namely 0.8, 0.4, or 0.2 g DHA/kg, and investigated the changes in LTB4/5 production by ionophore-activated PMN. The averaged LTB4 production by PMN was significantly reduced to 57 and 59% of baseline at 6 h after the infusion of 0.8 and 0.4 g DHA/kg, respectively (P < 0.05), but not after the infusion of 0.2 g DHA/kg or 0.8 g soybean oil/kg. The combined concentrations of both DHA and eicosapentaenoic acid in the PMN phospholipid fraction were significantly increased at 6 h after the infusion of 0.8 or 0.4 g DHA/kg but not after the infusion of 0.2 g DHA/kg or 0.8 g soybean oil/kg. Oral administration of 0.8 g DHA/kg did not increase DHA or eicosapentaenoic acid in the PMN phospholipid fraction and did not decrease LTB4 production by PMN at 6 h after administration. We suggest that the infusion of 0.4-0.8 g DHA/kg might be beneficial to patients who suffer from diseases that are related to the acute elevation of LTB4 production.
First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Japan.
N
N
Nakamura
T
T
Hamazaki
K
K
Yamazaki
H
H
Taki
M
M
Kobayashi
K
K
Yazawa
F
F
Ibuki
D000818:Animals; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D004655:Emulsions; D005227:Fatty Acids / Q000097:blood*; D005990:Glycerol / Q000008:administration & dosage / Q000378:metabolism*; D007262:Infusions, Intravenous; D007975:Leukotriene B4 / Q000378:metabolism* / Q000031:analogs & derivatives; D008055:Lipids / Q000097:blood; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D011817:Rabbits; D013024:Soybean Oil / Q000008:administration & dosage
D004655:Emulsions; D005227:Fatty Acids; D008055:Lipids; D007975:Leukotriene B4; D004281:Docosahexaenoic Acids; D013024:Soybean Oil; C040212:leukotriene B5; D015118:Eicosapentaenoic Acid; D005990:Glycerol