Delayed expression of in vivo restriction activity following conjugal transfer of Escherichia coli hsdK (restriction-modification) genes.
Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified host and suggests regulation of restriction activity after conjugal transfer.
Department of Microbiology, School of Medicine, Loma Linda University, California 92350.
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Prakash-Cheng
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Ryu
D003227:Conjugation, Genetic / Q000235:genetics / Q000502:physiology*; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology*
D004262:DNA Restriction Enzymes