A nucleotide sequence comparison of coxsackievirus B4 isolates from aquatic samples and clinical specimens.
Ten coxsackievirus B4 (CVB4) strains isolated from clinical and environmental sources in Northern Ireland in 1985-7, were compared at the nucleotide sequence level. Dideoxynucleotide sequencing of a polymerase chain reaction (PCR) amplified fragment, spanning the VP1/P2A genomic region, classified the isolates into two distinct groups or genotypes as defined by Rico-Hesse and colleagues for poliovirus type 1. Isolates within each group shared approximately 99% sequence identity at the nucleotide level whereas < or = 86% sequence identity was shared between groups. One isolate derived from a clinical specimen in 1987 was grouped with six CVB4 isolates recovered from the aquatic environment in 1986-7. The second group comprised CVB4 isolates from clinical specimens in 1985-6. Both groups were different at the nucleotide level from the prototype strain isolated in 1950. It was concluded that the method could be used to sub-type CVB4 isolates and would be of value in epidemiological studies of CVB4. Predicted amino acid sequences revealed non-conservation of the tyrosine residue at the VP1/P2A cleavage site but were of little value in distinguishing CVB4 variants.
Hughes
M S
MS
Department of Microbiology & Immunobiology, Queen's University of Belfast, Royal Victoria Hospital, N. Ireland.
Hoey
E M
EM
Coyle
P V
PV
D001483:Base Sequence; D002675:Child, Preschool; D003384:Coxsackievirus Infections / Q000453:epidemiology*; D004279:DNA, Viral / Q000235:genetics; D029822:Enterovirus B, Human / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009660:Northern Ireland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
D004279:DNA, Viral; D012367:RNA, Viral