Bombesin and muscarinic receptor activation in rat pancreas generate cyclic inositol monophosphate: possible involvement of different phospholipase C isoenzymes.
Phospholipase C isoenzymes can generate different proportions of cyclic and non-cyclic inositol phosphates. Stimulation of [3H]-inositol labeled pancreatic minilobules by buffer, bombesin, neuromedin B or carbachol in presence of 10 mM lithium, followed by separation of inositol phosphates, yielded the following results for cyclic inositol monophosphate (cIP) [DPM/mg protein; Mean +/- SEM (n)]: control [21 +/- 6, (9)]; bombesin [145 +/- 24, (12)]; neuromedin B (99 +/- 22 (9)] and carbachol [512 +/- 60, (12)]. The generation of cIP and IP were significantly correlated [r2 = 0.72 (p < 0.05)] following carbachol activation, while no significant correlation was obtained following bombesin receptor activation by either bombesin or neuromedin B. Presence of zinc (100 microM) in the final incubation medium failed to amplify the bombesin-stimulated cIP accumulation. Based on our studies we postulate that different phospholipase C isoenzymes may be activated following muscarinic and bombesin receptor stimulation in pancrea.
Department of Pathology, University of Alabama, Birmingham 35294.
M C
MC
Sekar
V
V
Sambandam
J M
JM
McDonald
D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D002217:Carbachol / Q000494:pharmacology; D007289:Cyclic IMP / Q000378:metabolism*; D004789:Enzyme Activation; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D015287:Neurokinin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018004:Receptors, Bombesin; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D007527:Isoenzymes; D018004:Receptors, Bombesin; D011976:Receptors, Muscarinic; D017981:Receptors, Neurotransmitter; D007289:Cyclic IMP; D007294:Inositol; D015287:Neurokinin B; C038393:neuromedin B; D002217:Carbachol; D010738:Type C Phospholipases; D015032:Zinc; D001839:Bombesin