Nourseothricin (streptothricin) inactivated by a plasmid pIE636 encoded acetyl transferase of Escherichia coli: location of the acetyl group.
Escherichia coli strains harbouring the plasmid pIE636 are able to synthesize acetylcoenzyme A: streptothricin acetyltransferase (ACSAT). The (enzymatic) N-acetylation of streptothricin F is known to contribute significantly towards the loss of antibacterial activity. 13C-NMR analysis of [14C]N-acetyl-labelled streptothricin F, produced by ACSAT-catalysed acetylation of streptothricin F and subsequent purification by various chromatographical steps, unequivocally revealed streptothricin F to be acetylated at the beta-amino group (C16) (and not at the epsilon-amino group (C19)).
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, FRG.
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U
Zähringer
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W
Voigt
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Seltmann
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D013309:Streptothricins / Q000096:biosynthesis* / Q000378:metabolism*
C009156:streptothricin F; C082678:N-beta-acetylstreptothricin F; D013309:Streptothricins; D000123:Acetyltransferases; C044496:streptothricin acetyltransferase