Simple and rapid determination of clomiphene cis and trans isomers in human plasma by high-performance liquid chromatography using on-line post-column photochemical derivatization and fluorescence detection.
A validated, sensitive and rapid high-performance high-performance liquid chromatographic method has been developed for the analysis of both cis and trans isomers of clomiphene in human plasma. The method involves a new off-line pre-column solid-phase extraction for sample preparation of clomiphene from human plasma in the presence of an internal standard. Analysis was performed by isocratic elution on a LiChrospher 100 RP-18 5-microns column using on-line post-column photochemical derivatization, with fluorescence detection at 247 nm (excitation) and 378 nm (emission). The limit of quantitation was 0.75 ng/ml for the cis isomer and 1.25 ng/ml for the trans isomer. The precision and accuracy of method were between good laboratory practice (GLP) required limits.
Urmös
I
I
Department of Pharmacokinetics, EGIS Pharmaceuticals Ltd., Budapest, Hungary.
Benkö
S M
SM
Klebovich
I
I
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002996:Clomiphene / Q000097:blood*; D006801:Humans; D007536:Isomerism; D010777:Photochemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D002996:Clomiphene