Enzyme-linked immunosorbent assay for rapid measurement of serum-neutralizing activity against human cytomegalovirus.
An enzyme-linked immunosorbent assay for rapid measurement of serum-neutralizing activity against human cytomegalovirus (CMV) was developed. The end point is quantitative and dependent on the colorimetric detection of viral early antigen in fixed monolayers. This assay avoids many of the drawbacks of other cytomegalovirus neutralization assays and is readily adaptable to large-scale screening.
Rabalais
G P
GP
Department of Pediatrics, University of Louisville School of Medicine, KY 40292.
Waldeyer
S
S
Cost
K
K
Marshall
G S
GS
D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D009500:Neutralization Tests / Q000379:methods*
D000914:Antibodies, Viral