Regulation of herpes simplex virus type 1 gene expression in nonpermissive murine resident peritoneal macrophages.
The restriction of herpes simplex virus type 1 (HSV-1) gene expression in nonpermissive murine resident peritoneal macrophages (ResPMO) was analyzed at the level of transcription. Using nuclear run-on assays, we established that transcription of a representative gene from each HSV-1 kinetic class was initiated in both ResPMO and permissive Vero cells. Using transient expression assays, we observed that the promoters of two early genes were activated by the immediate-early protein ICP4 in ResPMO. However, the level of transactivation of these promoters by another immediate-early protein, ICP0, was markedly reduced in ResPMO. Furthermore, the synergistic trans-activation normally observed between ICPs 4 and 0 in Vero cells was noticeably absent in ResPMO. The data indicate that the block in viral gene expression in ResPMO occurs after initiation of viral gene transcription and involves dysfunction of a viral immediate-early regulatory protein, ICP0.
Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia.
L
L
Wu
P S
PS
Morahan
K
K
Leary
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology*; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D007700:Kinetics; D008264:Macrophages / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D014709:Vero Cells
D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase