Expression of functional PDGF beta receptors in a human large-cell lung-carcinoma cell line.
In this study we have investigated a panel of lung-cancer cell lines, both of small-cell carcinoma and non-small-cell type, for the expression of receptors for platelet-derived growth factor. Although we found mRNA expression for the beta-type receptor on one small-cell and one non-small-cell line and alpha-type receptor mRNA expression on one small-cell-cancer cell line, only the beta receptors of the non-small-cell line (H-157) proved to be functional. Thus, the cell line H-157 displayed specific binding of 125I-PDGF-BB in addition to mRNA expression of the 6-kb transcript for the PDGF beta type receptor. Further evidence for the presence of functional PDGF beta receptors in H-157 cells was obtained from an in vitro kinase assay, which demonstrated a ligand-induced receptor autophosphorylation as well as the phosphorylation of a number of potential substrates associated with the activated receptor.
Department of Pathology, University of Uppsala, Sweden.
K
K
Forsberg
J
J
Bergh
B
B
Westermark
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011956:Receptors, Cell Surface / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor