Ochratoxin A in cow's milk and in human milk with corresponding human blood samples.
A method for determining ochratoxin A in milk has been elaborated in which the sample was subjected to a liquid-liquid extraction step and then purified on a silica gel column packed in a Pasteur pipet. The purified samples were analyzed by ion-pair liquid chromatography with fluorescence detection. The detection and quantitation limits for determination of ochratoxin A in cow's milk were 10 and 40 ng ochratoxin A/L milk, respectively. The same limits were valid for the analysis of human milk. A total of 36 cow's milk and 40 human milk samples were analyzed. All samples were collected in Sweden. Ochratoxin A was found in 5 (14%) of the cow's milk samples (range 10-40 ng/mL) and in 23 (58%) of the human milk samples (range 10-40 ng/L). Blood samples were collected from the mothers who gave milk samples. A total of 39 samples were analyzed. All blood samples contained ochratoxin A in concentrations exceeding the quantitation limit (60 ng/L blood). The mean concentration of ochratoxin A in the samples was 167 ng/L blood (range 90-940 ng/L). The concentration of ochratoxin A in human milk was < or = 0.1 of that in the human blood.
Breitholtz-Emanuelsson
A
A
Royal Institute of Technology, Department of Biochemistry and Biotechnology, Stockholm, Sweden.
Olsen
M
M
Oskarsson
A
A
Palminger
I
I
Hult
K
K
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry*; D009793:Ochratoxins / Q000032:analysis* / Q000097:blood; D013050:Spectrometry, Fluorescence; D013548:Sweden
D007202:Indicators and Reagents; D009793:Ochratoxins; C025589:ochratoxin A