Alternative labeling techniques for automated fluorescence based analysis of PCR products.
We present here convenient and simple methods that utilize two new fluorescent tags, TOTO-1 and fluorescein-12-dUTP, in conjunction with analysis of PCR products on automated, fluorescence-based electrophoretic instruments. These methods should prove valuable in those applications wherein the effort or expense of covalently attaching a fluorescent tag onto one or both of the PCR primers is not justified. Advantages and disadvantages of the new labeling methods are then reviewed.
Mansfield
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Applied Biosystems, Division of Perkin-Elmer, Foster City, CA 94404.
Kronick
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MN
D003856:Deoxyuracil Nucleotides; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D009136:Muscular Dystrophies / Q000235:genetics; D016133:Polymerase Chain Reaction; D011806:Quinolinium Compounds; D013844:Thiazoles
D003856:Deoxyuracil Nucleotides; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; D013844:Thiazoles; C069632:fluorescein-11-deoxyuridine triphosphate; C075295:1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-thiazole)-2-methylidene)quinolinium