Rapid detection of B virus (herpesvirus simiae) DNA by polymerase chain reaction.
Rapid diagnosis of B virus (herpesvirus simiae) infection in humans followed by early antiviral treatment is essential for the patient's survival. To improve laboratory diagnosis of B virus infections, a polymerase chain reaction (PCR)-based test using synthetic oligonucleotide primers and probe was developed to detect B virus DNA in clinical samples. After the specificity of the PCR was assessed for detection of several B virus isolates, the method was used to investigate human and monkey specimens, and results were compared with those obtained by viral culture. PCR appeared to be more sensitive than conventional virus isolation and thus of practical use for a rapid identification of B virus infection when conventional viral cultures are negative.
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78228.
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F
Scinicariello
R
R
Eberle
J K
JK
Hilliard
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D000882:Haplorhini; D006566:Herpesviridae Infections / Q000175:diagnosis*; D006568:Herpesvirus 1, Cercopithecine / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014779:Virus Replication
D004279:DNA, Viral; D015345:Oligonucleotide Probes