Immunohistochemical detection of aflatoxin B1-DNA adducts and hepatitis B virus antigens in hepatocellular carcinoma and nontumorous liver tissue.
Monoclonal antibodies recognizing the stable imidazole ring-opened form of the major N7-guanine aflatoxin B1-DNA adduct have been used in competitive enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays to quantitate adduct levels in liver tissue. Methods were developed in AFB1-treated animals, then applied to paired tumor and nontumor liver tissues of hepatocellular carcinoma patients from Taiwan. An avidin-biotin complex staining method was also used for of the detection of hepatitis B surface (HBsAg) and X (HBxAg) antigens in liver sections. A total of 8 (30%) hepatocellular carcinoma (HCC) samples and 7 (26%) adjacent nontumor liver tissue samples from Taiwan were positive for AFB1-DNA adducts. For HBsAg, 10 (37%) HCC samples and 22 (81%) adjacent nontumorous liver samples were positive, and 9 (33%) HCC samples and 11 (41%) adjacent nontumor liver samples were HBxAg positive. No association with AFB1-DNA adducts was observed for HBsAg and HBxAg. These methods should be useful in determining the role of exposure in the induction of HCC in Taiwan.
Santella
R M
RM
Comprehensive Cancer Center, School of Public Health, Columbia University, New York, NY 10032.
Zhang
Y J
YJ
Chen
C J
CJ
Hsieh
L L
LL
Lee
C S
CS
Haghighi
B
B
Yang
G Y
GY
Wang
L W
LW
Feitelson
M
M
D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000382:microbiology; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006511:Hepatitis B Antigens / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism / Q000382:microbiology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000382:microbiology; D051381:Rats; D015534:Trans-Activators / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
D018736:DNA Adducts; D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C072611:aflatoxin B1-DNA adduct; C057628:hepatitis B virus X protein; D004247:DNA; D016604:Aflatoxin B1