Quantitation of latent varicella-zoster virus DNA in human trigeminal ganglia by polymerase chain reaction.
Competitive polymerase chain reaction was used to quantitate latent varicella-zoster virus (VZV) DNA in human trigeminal ganglia. Ganglionic DNA from five subjects was amplified with oligonucleotide primers specific for VZV gene 28. Two of the samples were also analyzed with primers specific for VZV gene 62. Our results indicated that there are 6 to 31 copies of the VZV genome in every 100,000 ganglionic cells.
Mahalingam
R
R
Department of Neurology, University of Colorado School of Medicine, Denver 80262.
Wellish
M
M
Lederer
D
D
Forghani
B
B
Cohrs
R
R
Gilden
D
D
D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005814:Genes, Viral; D006562:Herpes Zoster / Q000175:diagnosis*; D014645:Herpesvirus 3, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012668:Trigeminal Ganglion / Q000382:microbiology*; D015678:Viral Structural Proteins / Q000235:genetics
D004279:DNA, Viral; D015678:Viral Structural Proteins