Autologous tumor cell killing activity of tumor-associated lymphocytes in patients with head and neck carcinomas.
In order to select the most cytotoxic effector cells for adoptive immunotherapy, lymphokine activated killer (LAK) cells, tumor infiltrating lymphocytes (TILs) and autologous mixed lymphocyte tumor cell culture (MLTC) cells derived from peripheral blood mononuclear cells (PBMC) in the same subject with head and neck carcinomas were prepared. The autologous tumor cell killing activity and cell surface phenotypes of each of the three effector cells were studied. MLTC cells cultured with interleukin-2 (IL-2) showed the strongest cytotoxic activity among these three different effector cells. Although TILs had suppressed killing activity immediately after isolation, after successive cultivations with IL-2, a cytotoxic activity against autologous tumor cells stronger than that of LAK cells appeared. Both IL-2 stimulated MLTC cells and TILs showed an enrichment of CD8 positive and CD11 negative cells in a CD3 positive subpopulation.
Department of Otorhinolaryngology. School of Medicine, Yokohama City University, Kanagawa, Japan.
M
M
Tsukuda
I
I
Mochimatsu
M
M
Sakumoto
S
S
Furukawa
S
S
Yuyama
S
S
Yanoma
A
A
Kubota
D000954:Antigens, Surface / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000097:blood / Q000276:immunology* / Q000628:therapy; D006801:Humans; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D010641:Phenotype; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D017252:CD3 Complex; D007376:Interleukin-2