Amplification of the Bacillus subtilis maf gene results in arrested septum formation.
The Bacillus subtilis homolog of the Escherichia coli morphogene orfE (of the mre operon) has been identified. The determinant is located on the chromosome immediately upstream of the mreBCD-minCD (divIVB) operon. The Maf protein shares substantial amino acid sequence identity with the E. coli OrfE protein. Introduction of the B. subtilis maf determinant on a multicopy plasmid into B. subtilis cells results in an inhibition of septation, which leads to extensive filamentation and loss of viability in the transformed cell population. Insertional inactivation of maf indicated that this gene is not essential for cell division.
Butler
Y X
YX
Department of Microbiology, Univeristy of Kansas, Lawrence 66045.
Abhayawardhane
Y
Y
Stewart
G C
GC
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005784:Gene Amplification; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C081169:Maf protein, Bacillus subtilis; D011994:Recombinant Proteins