Neuroanatomical alterations in the rat nucleus of the solitary tract following early maternal NaCl deprivation and subsequent NaCl repletion.
Restricting the NaCl content in the rat maternal and preweaning diet results in a significant and specific reduction (60%) of chorda tympani nerve responsiveness to sodium stimuli in the offspring. Repletion of dietary sodium at any time during postnatal development results in a complete and persistent recovery of chorda tympani nerve function. To learn whether the maturation of postsynaptic cells are also affected by the early dietary manipulation, dendritic morphology, neuronal and glial densities and numbers were studied within the area of the nucleus of the solitary tract (NTS). Examination of dendritic morphologies in Golgi-Cox stained neurons revealed that cells with multipolar and fusiform somata in the rostral NTS exhibited longer dendrites following dietary NaCl deprivation during development (deprived rats) than in controls. These changes were generally maintained in rats initially deprived of NaCl and then fed a NaCl-replete diet postweaning ("recovered" rats). In contrast, ovoid neurons were not affected by NaCl deprivation but had increases in the lengths of their dendrites following "recovery." Along with dendritic alterations, the packing density of neurons in the rostral NTS was greater in NaCl-deprived rats than in controls, but was similar to controls following "recovery." Glial packing density also increased following deprivation and remained high in "recovered" rats. These results indicate that activity-dependent events as well as events not related to afferent activity (e.g., hormonal changes) may influence the morphological development of NTS neurons. In addition, significant interactions among primary afferent fibers, central neurons, and glia may direct development within the central gustatory system.
King
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Department of Psychology, University of Virginia, Charlottesville 22903.
Hill
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D000818:Animals; D004039:Diet, Sodium-Restricted / Q000009:adverse effects*; D005260:Female; D006651:Histocytochemistry; D008526:Medulla Oblongata / Q000033:anatomy & histology* / Q000166:cytology / Q000187:drug effects; D009457:Neuroglia / Q000187:drug effects / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D012982:Sodium, Dietary / Q000494:pharmacology*; D013649:Taste / Q000187:drug effects / Q000502:physiology
D012982:Sodium, Dietary