Preliminary crystallographic analysis of bovine enterovirus.
Bovine enterovirus (BEV) strain VG-5-27 derived from an infectious cDNA clone has been crystallized as extended hexagonal plates. Virus recovered from crystals produced cytopathic effect in BHK cells. These crystals diffract X-rays from high energy synchrotron sources to beyond 2.7 A. The crystal system is monoclinic, space group P2(1) with unit cell dimensions a = 388 A, b = 390 A, c = 360 A, beta = 113 degrees. The virion is 300 A in diameter and one whole particle constitutes the crystallographic asymmetric unit, giving rise to 60-fold non-crystallographic redundancy.
Laboratory of Molecular Biophysics, Oxford, U.K.
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Smyth
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Martin
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004770:Enterovirus / Q000302:isolation & purification / Q000648:ultrastructure*; D014961:X-Ray Diffraction