Crystallization and preliminary crystallographic studies of human beta-glucuronidase.
Crystals of human beta-glucuronidase have been obtained by the vapor diffusion method, using 2-methyl-2,4-pentanediol as a precipitant. The crystals belong to the orthorhombic space group P222(1), with cell dimensions a = 134.5 A, b = 95.1 A, c = 124.4 A. The unit cell contains two copies of the tetrameric enzyme. Complete native data have been collected to a resolution of 2.6 A.
E. A. Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, MO 63104.
W B
WB
Drendel
J H
JH
Grubb
W S
WS
Sly
Z
Z
Chen
F S
FS
Mathews
S
S
Jain
D005966:Glucuronidase / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D005966:Glucuronidase