[Production of maedi-visna virus antigen for the immunodiffusion test and its use for field studies].
Using the Maedi-Visna virus strain WLC1 (Weybridge) and different cell lines of sheep plexus chorioideus we were able to establish and improve a production procedure of MV-antigen for use in immunodiffusion assay. Our attention was focused mainly on the efficient virus multiplication in cell cultures and on standardisation of the procedure to find a method keeping the antigen loss as low as possible. Investigations with our antigen in 39 farms of 5 of the former districts in East Germany revealed a seropositive reagent rate between 0 and more than 60%, underlining the need for a complex diagnostic and eradication programme.
Friedrich-Loeffler-Institut Insel Riems.
E
E
Starick
L
L
Mewes
H
H
Bergmann
R
R
Riebe
C
C
Schaepe
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D002460:Cell Line; D002831:Choroid Plexus / Q000166:cytology; D005779:Immunodiffusion / Q000662:veterinary*; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000175:diagnosis*; D012756:Sheep; D014790:Visna-maedi virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral