Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR.
The amplification of the 468 bp fragment of the BHV-1 genome by PCR is described. The 22 bp oligomers from the BHV-1 gI gene were used as primers. For successful amplification the thermal denaturation (100 degrees C/8 min, ice) of the DNA sample was carried out prior to the cycling (95 degrees C for 1 min, 56 degrees C for 1 min, 73 degrees C for 1 min). The presence of glycerol markedly enhances the PCR.
Vilcek
S
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Joint Laboratory of Genetic Manipulations, University of Veterinary Medicine, Kosice, Czechoslovakia.
D001483:Base Sequence; D004277:DNA, Single-Stranded; D016679:Genome, Viral; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics
D004277:DNA, Single-Stranded; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins