Augmented inhibition of MethA tumor cell proliferation in combined use of diethyldithiocarbamate with catalase or by a nondialysable fraction from co-incubation.
The antiproliferative effect of diethyldithiocarbamate (DDC), a metal chelator, in combined use with catalase on MethA tumor cells was studied. Marked augmentation of the antiproliferative effect was observed when 1 x 10(-7) M DDC was used in combination with catalase (0.004-40 micrograms/ml). Further augmentation of the cytostatic effect was obtained by the simultaneous addition of 2 x 10(-7) M DDC with catalase and more than 97% inhibition of [3H]thymidine uptake by target cells was observed. A nondialysable fraction from the co-incubation of DDC with catalase was also remarkably cytostatic to the target cells. Serum factor(s), probably metal ions, was suggested to be necessary for the induction of the nondialysable fraction with cytostatic activity. The activity was not nullified by the pretreatment of a nondialysable fraction with anti-catalase antibody. These results suggest that an active substance or compound exhibiting an antiproliferative effect on tumor cells might be newly formed as the result of the interaction of DDC with catalase.
Mashiba
H
H
Division of Immunology, National Kyushu Cancer Center, Fukuoka, Japan.
Matsunaga
K
K
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002374:Catalase / Q000276:immunology / Q000627:therapeutic use*; D002455:Cell Division / Q000187:drug effects; D003956:Dialysis; D004050:Ditiocarb / Q000627:therapeutic use*; D004347:Drug Interactions; D008748:Methylcholanthrene / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000906:Antibodies; D008748:Methylcholanthrene; D004050:Ditiocarb; D002374:Catalase