Changes in the expression of guanine nucleotide-binding proteins during differentiation of 3T3-F442A cells in a hormonally defined medium.
Using specific antisera, the expression of the G protein alpha subunits, G8, G(i1), G(i2), G(i3) and G0, were determined in 3T3-F442A cells during their differentiation to adipocytes in a hormonally defined medium. Differentiation caused distinct increases in the expression of two Gs isoforms and decreases in the expression of both G(i2) and G(i3). Differentiation also resulted in a 2- to 4-fold increase in forskolin-stimulated adenylyl cyclase activity and a 15-fold increase in the response of cells to a beta-adrenergic agonist. The increase in Gs expression was also observed, to a lesser degree, in cells maintained at confluence under conditions where morphological conversion was negligible and the decreased expression of G(i2) and G(i3) and the increased beta-adrenergic responsiveness did not occur.
Hannah Research Institute, Ayr, Scotland, UK.
E
E
Kilgour
N G
NG
Anderson
D016475:3T3 Cells; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D016895:Culture Media, Serum-Free; D000242:Cyclic AMP / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006728:Hormones / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice
D016895:Culture Media, Serum-Free; D006728:Hormones; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D007545:Isoproterenol