Stereoselective distribution of acenocoumarol enantiomers in human plasma: chiral chromatographic analysis of the ultrafiltrates.
Stereoselectivities for the binding of rac-acenocoumarol to human serum albumin (HSA), alpha 1-acid glycoprotein (AGP), and human plasma were determined by chiral HPLC analysis of the ultrafiltrates on a Chiral-AGP column. The results confirmed the previously detected inverse stereoselectivities; for HSA the ratio of the enantiomeric constants was KR/KS approximately 2, while for AGP it was KR/KS approximately 0.3. In plasma the contribution of HSA dominates, although in pathological states, elevated AGP levels may compensate for stereoselective distribution.
Fitos
I
I
Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.
Visy
J
J
Simonyi
M
M
Hermansson
J
J
D000074:Acenocoumarol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008024:Ligands; D009961:Orosomucoid / Q000737:chemistry; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014462:Ultrafiltration
D008024:Ligands; D009961:Orosomucoid; D012709:Serum Albumin; D000074:Acenocoumarol