Use of Percoll gradients for isolation of human placenta mitochondria suitable for investigating outer membrane proteins.
Human mitochondria were isolated from placenta by a combination of differential and Percoll gradient centrifugation, resulting in a highly pure and intact preparation as assessed by marker enzyme analysis and electron microscopy. The advantages over previous methods are the rapidity of the procedure and the excellent resolution of mitochondria and lysosomes. Moreover, the high extent of intactness of the mitochondria so obtained made them particularly well suited for investigating outer membrane proteins. Taking advantage of this method, we have purified human mitochondrial porin. The purified protein consists of a single unglycosylated polypeptide of molecular mass 33 kDa.
Gasnier
F
F
Department of Biochemistry, INSERM-CNRS 189, Lyon-Sud Medical School, Oullins, France.
Rousson
R
R
Lermé
F
F
Vaganay
E
E
Louisot
P
P
Gateau-Roesch
O
O
D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000737:chemistry* / Q000648:ultrastructure; D008970:Molecular Weight; D010920:Placenta / Q000737:chemistry* / Q000648:ultrastructure; D018272:Porins; D011205:Povidone; D011247:Pregnancy; D012822:Silicon Dioxide; D050994:Voltage-Dependent Anion Channels
D008565:Membrane Proteins; D018272:Porins; D050994:Voltage-Dependent Anion Channels; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone