Isolation and characterization of a rice cDNA which encodes a ubiquitin protein and a 52 amino acid extension protein.
We isolated a rice cDNA clone encoding the ubiquitin protein fused to a ribosomal protein. This clone encodes a single ubiquitin polypeptide and extension protein of 53 amino acids. This extension protein shows a high degree of homology with those of the yeast ubil or ubi2 gene, both of which encode the same protein. Northern blot analysis suggested that the expression pattern of this gene is more similar to other ribosomal protein genes not linked to ubiquitin protein than to the polyubiquitin gene.
Department of Botany, Faculty of Science, Hokkaido University, Sapporo, Japan.
R
R
Nishi
H
H
Hashimoto
S
S
Kidou
H
H
Uchimiya
A
A
Kato
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014452:Ubiquitins / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D014452:Ubiquitins