Molecular cloning reveals the existence of a fourth gamma subunit of the vertebrate brain GABAA receptor.
We have isolated a cDNA, from the chicken, that encodes a fourth type of gamma subunit of the vertebrate brain GABAA receptor. The mature polypeptide (which we name gamma 4) displays 67%, 69% and 70% identity, respectively, to the rat gamma 1, gamma 2 and gamma 3 subunits. In the developing chicken brain, the gamma 4-subunit mRNA is first detected at embryonic day 13; the transcript level then increases progressively during embryogenesis. In situ hybridization reveals that the gamma 4-subunit mRNA is abundant in several brain regions, including the ectostriatum, nucleus rotundus and hyperstriatum ventrale, which are involved in visual processing and learning.
Harvey
R J
RJ
Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, Germany.
Kim
H C
HC
Darlison
M G
MG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology; D002642:Chick Embryo; D003001:Cloning, Molecular; D015870:Gene Expression; D005796:Genes; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011963:Receptors, GABA-A / Q000235:genetics*; D016415:Sequence Alignment
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011963:Receptors, GABA-A