Identification of the Epstein-Barr virus nuclear antigen 2 transactivation domain.
The Epstein-Barr virus nuclear antigen 2 (EBNA2) protein activates the expression of viral and cellular genes. A set of seven yeast GAL4-EBNA2 fusion proteins were constructed in order to identify the transactivation domain of EBNA2. These fusion proteins were tested for their ability to transactivate a target gene in Hela and BJAB cells. This analysis has demonstrated that the EBNA2 polyproline domain is dispensable for transactivation while the acidic carboxy terminus defined by amino acids 337-467 is essential. This result is consistent with the analysis of a variety of viral and cellular eucaryotic transactivators in which an acidic domain of the protein has been shown to be indispensible for function.
Horvath
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GC
Department of Medicine, State University of New York, Stony Brook 11794-8174.
Schubach
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WH
D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation
D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D015500:Chloramphenicol O-Acetyltransferase