Cloning of a human platelet-activating factor receptor gene: evidence for an intron in the 5'-untranslated region.
A clone encoding a gene for a human platelet-activating factor (PAF) receptor has been isolated from a human genomic library. A 6-kb Hind III fragment was subcloned and was found to contain a full coding sequence identical with that previously reported for cDNA clones encoding PAF receptors from leukocyte cDNA libraries. Sequencing of the 6-kb Hind III fragment upstream from the start codon revealed that the 5'-untranslated region deviated from reported cDNA sequences beginning at base -39, suggesting the presence of an intron in this region. Consensus sequences for a splice junction appear appropriately located at the predicted 3' end of the purported intron. Restriction map analysis of the region revealed that the size of the intron in the 5'-untranslated region was at least 16 kb. These data indicate that a gene for a human PAF receptor is present in the genome without introns in the coding sequence and that splicing of mRNA encoding PAF receptors appears to occur in the 5'-untranslated region.
Department of Pharmacology, University of Arizona Health Sciences Center, Tucson 85724.
P B
PB
Chase
M
M
Halonen
J W
JW
Regan
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics; D015698:Genomic Library; D006168:Guinea Pigs; D006801:Humans; D007438:Introns; D007962:Leukocytes / Q000502:physiology; D008168:Lung / Q000502:physiology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010972:Platelet Activating Factor / Q000378:metabolism; D010980:Platelet Membrane Glycoproteins; D016133:Polymerase Chain Reaction / Q000379:methods; D014176:Protein Biosynthesis; D011956:Receptors, Cell Surface / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D009838:Oligodeoxyribonucleotides; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D004247:DNA