Bromodeoxyuridine-labelled apoptosis after treatment with antimetabolites in two murine tumours and in small intestinal crypts.
Antimetabolites are S-phase specific anticancer drugs. Administration of bromodeoxyuridine (BrdUrd) to tumour bearing mice was followed by treatment with cytosine arabinoside or hydroxyurea. Anti-BrdUrd immunocytochemistry visualised susceptible tumour and intestinal crypt cells at electron microscope level, showing unequivocally that cells that were in S-phase at the time of administration of the drugs subsequently died by apoptosis.
Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
C E
CE
Sarraf
T W
TW
Ansari
P
P
Conway
M
M
Notay
S
S
Hill
M R
MR
Alison
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001973:Bromodeoxyuridine; D002277:Carcinoma / Q000188:drug therapy / Q000473:pathology*; D003561:Cytarabine / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008940:Mitotic Index / Q000187:drug effects; D016196:S Phase; D012513:Sarcoma, Experimental / Q000188:drug therapy / Q000473:pathology*
D003561:Cytarabine; D001973:Bromodeoxyuridine; D006918:Hydroxyurea