Testosterone, dihydrotestosterone, trenbolone acetate, and zeranol alter the synthesis of cortisol in bovine adrenocortical cells.
The objective of this study was to examine the effect of anabolic steroids (testosterone, T; dihydrotestosterone, D; trenbolone acetate, B; and zeranol, Z) on cortisol synthesis by cultured bovine adrenocortical cells. Adrenal glands were obtained from slaughter-aged steers (n = 4). Cortical cells were isolated and their steroidogenic capacity was examined. They were plated in multiwell culture plates. At confluence, cells were treated with T, D, B, or Z at 0, 10, 50, 125, or 500 ng/mL (eight wells per treatment). Twenty-four hours after treatment, one-half of each treatment concentration was stimulated with 10(-9) M ACTH. After 8 h of incubation, cortisol concentration in the media was measured using RIA. Cortical cells were removed from the plates using 1 mM EDTA and analyzed for DNA content. Data were subjected to rank transformation and analyzed by randomized complete block design. Adrenocorticotropic hormone stimulated (P < .01) the release of cortisol by more than threefold. Cortisol synthesis was lower (P < .05) in the presence of T, D, and B. Testosterone caused a greater (P < .05) suppression in cortisol production at 50 and 125 ng/mL than did D. The suppression of cortisol synthesis did not differ between B and T or between B and D. Cortisol synthesis was lowered (P < .05) by the presence of T, D, and B in both ACTH-stimulated and nonstimulated cells but was only suppressed in ACTH-stimulated cells of Z-treated cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Isaacson
W K
WK
Department of Animal Science, University of Nebraska, Lincoln 68583-0908.
Jones
S J
SJ
Krueger
R J
RJ
D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D045930:Anabolic Agents / Q000494:pharmacology*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D013196:Dihydrotestosterone / Q000494:pharmacology; D006854:Hydrocortisone / Q000096:biosynthesis*; D008297:Male; D011897:Random Allocation; D013739:Testosterone / Q000494:pharmacology; D014204:Trenbolone Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D015029:Zeranol / Q000494:pharmacology
D045930:Anabolic Agents; D013196:Dihydrotestosterone; D013739:Testosterone; D015029:Zeranol; D000324:Adrenocorticotropic Hormone; D004247:DNA; D014204:Trenbolone Acetate; D006854:Hydrocortisone