Some biochemical properties of an acyclic oligonucleotide analogue. A plausible ancestor of the DNA?
As acyclic oligonucleotides have been suggested as a primitive model of DNA or RNA in prebiotic times, we compared some biochemical properties of these analogues to that of natural ones. Firstly, an acyclic analogue of deoxyribonucleoside triphosphates was tested as a potential substrate of enzymes intervening in nucleic acids synthesis. GlyTTP, a dTTP analogue with a missing 2'-methylene group is not accepted as a substrate by either DNA polymerase or deoxynucleotidyl terminal transferase (TdT). Secondly, the modified dodecathymidylate (GlyT)12, the racemic acyclic sugar analogue of (dT)12, proved to be an efficient primer for DNA polymerase and TdT, though the associative properties of (GlyT)12 are very weak as shown by UV spectroscopy in phosphate buffer without magnesium chloride. But (GlyT)12 has the advantage to be 500-times more stable against hydrolysis by snake venom phosphodiesterase than the corresponding oligothymidylate.
Polymères, Biopolymères, Membranes, URA 500 du CNRS, Université de Rouen, Mont-Saint-Aignan, France.
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Merle
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Spach
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Merle
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Sági
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Szemzö
D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006868:Hydrolysis; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity; D013942:Thymine Nucleotides / Q000378:metabolism*
D009694:Nucleic Acid Precursors; D013942:Thymine Nucleotides; D004259:DNA-Directed DNA Polymerase; D010727:Phosphoric Diester Hydrolases; C024157:thymidine 5'-triphosphate