Cytoplasmic poly(ADP-ribose)polymerase from mouse plasmacytoma free messenger ribonucleoprotein particles: purification and characterization.
A cytoplasmic poly(ADP-ribose)polymerase (PARP) was purified from mouse plasmacytoma free messenger ribonucleoprotein particles using chromatography on 3-aminobenzamide affigel-10. The purified protein showed one band at 116 kDa on SDS-polyacrylamide gel electrophoresis and shared similar antigenic sites to the nuclear PARP. An apparent Km for NAD of 100.5 +/- 6.3 microM and a Vmax of 174 +/- 40 nmoles of ADP-ribose incorporated/min/mg protein were observed. RNA was detected in the enzyme preparation and the enzymatic activity was not DNA dependent.
Centre de Neurochimie du C.N.R.S., Strasbourg, France.
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M
Jesser
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C
Chypre
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F
Hog
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Mandel
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002846:Chromatography, Affinity; D003593:Cytoplasm / Q000201:enzymology; D004247:DNA / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009536:Niacinamide / Q000494:pharmacology; D010954:Plasmacytoma / Q000201:enzymology*; D011065:Poly(ADP-ribose) Polymerases / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D013936:Thymidine / Q000494:pharmacology
D001549:Benzamides; D012333:RNA, Messenger; D009536:Niacinamide; C025160:3-aminobenzamide; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D008274:Magnesium; C025159:3-methoxybenzamide; D002118:Calcium; D013936:Thymidine