Meganuclease I-SceI as a tool for the easy subcloning of large DNA fragments devoid of selection marker.
Viret
J F
JF
Swiss Serum and Vaccine Institute, Berne.
D001709:Biotechnology; D003001:Cloning, Molecular / Q000379:methods*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D029701:Saccharomyces cerevisiae Proteins; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific