Molecular cloning of crustacean red pigment concentrating hormone precursor.
A cDNA encoding the precursor of the red pigment concentrating hormone (RPCH) of the shore crab, Carcinus maenas, was isolated and sequenced. The predicted preprohormone sequence consists of a putative 25 amino acid signal peptide, the eight amino acid RPCH sequence followed by a Gly as amide donor, a dibasic processing site and a 74 residue peptide of unknown function, referred to as RPCH-precursor-related peptide (RPRP). In in situ hybridisation experiments, the RPCH gene transcript could be localised in two clusters of neurons in the medulla terminalis of the eyestalk.
Institut für Zoophysiologie, University of Bonn, Germany.
B
B
Linck
J M
JM
Klein
S
S
Mangerich
R
R
Keller
W M
WM
Weidemann
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000378:metabolism*; D003001:Cloning, Molecular; D015723:Gene Library; D017403:In Situ Hybridization; D007447:Invertebrate Hormones / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D009842:Oligopeptides / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*
D007447:Invertebrate Hormones; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D009842:Oligopeptides; D011498:Protein Precursors; D012333:RNA, Messenger; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid