[Analytic and preparative laser scanning cytometry].
Laser scanning cytometry for analysis and preparation is viewed by some as a blend between flow cytometry and image analysis, since it allows to measure and localize fluorescence and to obtain morphologic or morphometric information on adherent or suspended cells or tissues. However, laser scanning cytometry has additional capabilities such as kinetic studies (slow or rapid) of live cells or measurement of fluorescence recovery after photoblinding. Most of these studies can be performed with great accuracy on localized zones by adding a confocal microscope system and performing three-dimensional image reconstruction. Studies on some of the novel possibilities of laser scanning cytometry are very scant.
Institut Pasteur, SC9 INSERM-Pasteur, Paris, France.
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Métézeau
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Kiefer
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Lamy
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Delamare
D015220:Calcium Channels; D002469:Cell Separation; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans
D015220:Calcium Channels; D005456:Fluorescent Dyes