The analysis of egg wash water for the rapid assessment of microbiological quality.
A total of 101 egg wash water samples from five different egg grading stations in eastern Ontario were analyzed for a variety of physical and chemical variables in an attempt to find a correlation with total bacterial counts. Temperature, pH, total chlorine, and percentage transmission at 600 nm (%T) were found to be significant variables, and a multiple regression equation was derived that accounted for 65% of the total variation. The equation was used to classify wash water samples as acceptable (< or = 10(5) cfu/mL) or unacceptable and correctly classified 77.2% of the samples. Classification of a second (validation) data set from 58 wash water samples was correctly predicted in 72% of the cases. The predictive value of the equation was especially good for those wash water samples obtained from stations that had used a chlorinated alkaline detergent, 90.4 and 100% for the modeling and validation data, respectively. Maintenance of wash water at recommended levels for temperature and pH (i.e., > or = 40 C and pH > or = 10) was insufficient to ensure bacterial numbers would be < or = 10(5) cfu/mL. Under normal operating conditions a minimum total available chlorine concentration of .45 mg/L should be maintained in wash water to ensure that bacterial numbers are kept at an acceptable level. Monitoring of temperature, pH, total chlorine, and %T will assist in maintaining wash water quality and minimize the number of samples returned to laboratories for microbiological analysis.
Centre for Food and Animal Research, Agriculture Canada, Ottawa, Ontario.
F M
FM
Bartlett
J M
JM
Laird
C L
CL
Addison
R C
RC
McKellar
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D002645:Chickens; D002713:Chlorine; D004531:Eggs / Q000382:microbiology; D005511:Food Handling / Q000592:standards*; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D012044:Regression Analysis; D013696:Temperature; D014867:Water / Q000737:chemistry; D014871:Water Microbiology
D014867:Water; D002713:Chlorine