Fast Na+ current in circular smooth muscle cells of the large intestine.
Whole-cell voltage clamp was carried out on freshly dispersed single smooth muscle cells from adult rat and human colons to investigate the regulation of the Ca2+ channels. In this study, we unexpectedly discovered the existence of a fast Na+ channel current. With normal physiological salt solution (PSS) plus 4-amino-pyridine (3 mM) in the bath and high-Cs+ solution in the pipette to inhibit outward K+ currents, an inward current possessing fast and slow components was observed when the cell membrane was depolarized to a value more positive than -20 mV from a holding potential of -100 mV. When Ca2+ ions were removed from the PSS, or when nifedipine (10 microM) and Ni2+ (30 microM) were simultaneously applied, the slow component disappeared and the fast component remained. The fast current component became almost completely inactivated within 10 ms. This fast component was dependent on extracellular Na+ concentration and was inhibited by tetrodotoxin (TTX) dose dependently (IC50 of 130 nM in rat and 14 nM in human). These results suggest that the slow component of inward current was a Ca2+ channel current, whereas the fast component was a TTX-sensitive fast Na+ channel current. The threshold voltage, the voltage for peak current, and the reversal potential for the fast Na+ current were, respectively, about -50, -20, and +50 mV in rats, and -40, 0, and +60 mV in humans. The incidence of cells possessing fast Na+ currents depended on the region of the colon.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Physiology and Biophysics, University of Cincinnati College of Medicine, OH 45267-0576.
Z
Z
Xiong
N
N
Sperelakis
A
A
Noffsinger
C
C
Fenoglio-Preiser
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002794:Choline / Q000494:pharmacology; D003106:Colon / Q000378:metabolism*; D004594:Electrophysiology; D006801:Humans; D066298:In Vitro Techniques; D008345:Manganese / Q000494:pharmacology; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D009532:Nickel / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000494:pharmacology; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D015220:Calcium Channels; D015222:Sodium Channels; D008345:Manganese; D013779:Tetrodotoxin; D009532:Nickel; D012964:Sodium; D009543:Nifedipine; D002794:Choline; D002118:Calcium