Structure and function of endoglucanase V.
Cellulose is the major polysaccharide component of plant cell walls and is the most abundant organic compound on the planet. A number of bacterial and fungal organisms can use cellulose as a food source, possessing cellulases (cellobiohydrolases and endoglucanases) that can catalyse the hydrolysis of the beta-(1,4) glycosidic bonds. They can be classified into seven distinct families. The three-dimensional structures of members of two of these families are known. Here we report the structure of a third cellulase, endoglucanase V, whose sequence is not represented in any of the above families. The enzyme is structurally distinct from the previously determined cellulases but is similar to a recently characterized plant defence protein. The active site region resembles that of lysozyme, despite the lack of structural similarity between these two enzymes.
Davies
G J
GJ
Department of Chemistry, University of York, Heslington, UK.
Dodson
G G
GG
Hubbard
R E
RE
Tolley
S P
SP
Dauter
Z
Z
Wilson
K S
KS
Hjort
C
C
Mikkelsen
J M
JM
Rasmussen
G
G
Schülein
M
M
D001665:Binding Sites; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D003196:Computer Graphics; D003904:Mitosporic Fungi / Q000201:enzymology; D009113:Muramidase / Q000737:chemistry; D011487:Protein Conformation; D014961:X-Ray Diffraction
D009113:Muramidase; D002480:Cellulase