Monoclonal antibodies that disrupt poliovirus only at fever temperatures.
Three of thirty-six monoclonal antibodies were found to cause irreversible inactivation of type 1 poliovirus at 39 degrees C but not at 37 degrees C. Neutralization at 37 degrees C depended on aggregation and was reversible by acid-induced deaggregation; at 39 degrees C, the virions (N antigenic, 160S) were disrupted to empty capsids (H antigenic, 100S), and neutralization was irreversible. The rate of antibody-dependent conversion of N to H antigen increased steeply between 37 and 39 degrees C.
Delaet
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Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Boeyé
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D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology*; D005334:Fever / Q000503:physiopathology*; D006801:Humans; D007700:Kinetics; D051379:Mice; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*; D013696:Temperature; D014633:Valine / Q000378:metabolism; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014633:Valine