Proteins specifically binding to the 3' untranslated region of hepatitis A virus RNA in persistently infected cells.
Establishment of persistency is the common result of hepatitis A virus (HAV) infection in most HAV/cell culture systems. Previous studies provided evidence that shortly before or concomitantly with establishment of persistent infections synthesis of viral RNA is down-regulated. This may be an effect of regulating factors. Using RNA/protein binding assays it was shown that, at the critical time during virus replication, proteins accumulate which interact specifically with a distinct nucleotide sequence (HPE) within the 3' non-coding region of the HAV genome and/or (HME) within the 5' terminal region of the HAV antigenome. The sequences consist of 23 nucleotides (HPE: 5'-AAAUUUUCUUAAAAUUUCUGAGG-3'; HME: 5'-CCUCAGAAAUUUUAAGAAAAUUU-3'). A sequence with 79% similarity was found in the corresponding 3' non-coding region of poliovirus type I (Sabin) RNA. The latter sequence was shown to bind proteins from HAV infected cells but comparable proteins were absent in cells infected poliovirus.
Yale New Haven Hospital, Connecticut.
J P
JP
Nüesch
M
M
Weitz
G
G
Siegl
D001483:Base Sequence; D002478:Cells, Cultured; D006507:Hepatovirus / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000235:genetics; D011485:Protein Binding; D016372:RNA, Antisense / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
D016372:RNA, Antisense; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014764:Viral Proteins