Mesangial kinetics after partial nephrectomy in rats fed a normal or a low-protein diet.
Mesangial kinetics of aggregated BSA (aggBSA) were studied in rats subjected to 1 1/3 nephrectomy (4/6 Nx) fed either a normal (16%) or a low-protein (8%) diet. At 4 weeks postsurgery, ablated rats exhibited modest elevations in blood pressure but had neither significant proteinuria nor evidence of glomerular damage. Following injection of aggBSA into 4/6 Nx rats (16% diet) there was a marked increase in mesangial localization at 4 h compared with control animals, followed by rapid clearance over 4-8 h, in keeping with increased trafficking in this model. The increased trafficking was significantly reduced by dietary protein restriction with 4/6 Nx rats fed an 8% protein diet exhibiting mesangial kinetics not significantly different from control animals. Control animals exhibited identical kinetics irrespective of diet. In separate studies, the 8% protein diet significantly reduced proteinuria and glomerulosclerosis when compared with untreated animals when studied at 30 weeks postsurgery.
Odum
J
J
Renal Research Laboratories, School of Postgraduate Medicine, University of Keele, Stoke-on-Trent, UK.
Purkiss
J
J
Naish
P F
PF
D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage*; D005920:Glomerular Mesangium / Q000378:metabolism*; D008297:Male; D009392:Nephrectomy; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics
D004044:Dietary Proteins; D012710:Serum Albumin, Bovine