[Human embryo freezing with dimethylsulfoxide (DMSO) and sucrose as cryoprotectants].
176 supernumerary human embryos following treatment by IVF were cryopreserved with dimethylsulfoxide (1.5M) and sucrose (0.1M) as cryoprotectants. The embryos were frozen at the early cleavage stage (48-96 hours after insemination) in the programmable freezer, by means of the protocol providing for slow cooling until -40 degrees C and slow thawing. The survival rate (50% or more blastomeres were intact) after thawing was higher for the embryos at the 8 cell stage to the morulla stage (60-96 hours after insemination) than for the embryos at the 4 to 6 cell stage (48-60 hours after insemination) (87% and 66%, p < 0.001), and the overall survival rate was 80% (140/176). Out of 62 transfer cycles, 11 pregnancies (17.7%) and 6 deliveries (9.7%) were achieved. No fetal malformation or chromosomal aberration was observed.
Department of Obstetrics and Gynecology, Ogikubo Hospital, Tokyo.
N
N
Kuji
M
M
Sakaida
T
T
Miyazaki
E
E
Katayama
T
T
Sugiyama
E
E
Iida
D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011247:Pregnancy; D013395:Sucrose / Q000494:pharmacology*; D014022:Tissue Survival
D003451:Cryoprotective Agents; D013395:Sucrose; D004121:Dimethyl Sulfoxide