Effects of repetitive motion on human fibroblasts.
Repetitive motion injuries such as tendonitis are common sports injuries. However, few scientific studies are available on the effects of repetitive motion on mesenchymal cells and the presumed inflammatory response. This study used a new in vitro model to study the effects of repetitive motion. Human tendon fibroblasts were subcultured and plated on culture wells with flexible bottoms. The cells were repetitively stretched using a micro-processor-controlled pressure unit that causes a cyclic deformation of the flexible bottom. The wells were divided in the following groups: group I controls without repetitive motion, group IIA repetitive motion with 0.25 strain at 0.17 Hz (10 cycles.min-1), group IIB repetitive motion with 0.25 strain and 0.17 Hz in presence of 25 microM indomethacin, and group III repetitive motion with 0.25 strain at 1 Hz (60 cycles.min-1). After 3 h of stimulation the supernatant fluids were harvested and evaluated for prostaglandin E2 (PGE2), leukotriene B4 (LTB4), and lactate dehydrogenase (LDH). The results showed significantly (P < 0.001) increased levels of PGE2 in groups IIA (46.9 +/- 4.7 pg.0.1 ml-1) and III (65.7 +/- 8.0 pg.0.1 ml-1). This represents a 1.3- and 1.8-fold increase, respectively, compared with the control group I (36.4 +/- 5.9 pg.0.1 ml-1). LTB4 was significantly (P < 0.001) elevated in the indomethacin-treated group IIB (45.0 +/- 11.0 pg.0.1 ml-1) compared with very low levels in all other groups. LDH was not significantly different in any of the experimental groups compared with the control group I. The results indicate that repetitive motion induces production of PGE2.(ABSTRACT TRUNCATED AT 250 WORDS)
Division of Orthopaedic Surgery, University of North Carolina, Chapel Hill 27599-7055.
L C
LC
Almekinders
A J
AJ
Banes
C A
CA
Ballenger
D000704:Analysis of Variance; D002478:Cells, Cultured; D012090:Cumulative Trauma Disorders / Q000378:metabolism* / Q000473:pathology; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007249:Inflammation / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007975:Leukotriene B4 / Q000096:biosynthesis*; D013710:Tendons / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
D007975:Leukotriene B4; D007770:L-Lactate Dehydrogenase; D015232:Dinoprostone; D007213:Indomethacin