Rat pleural and peritoneal mast cells stimulated at different cellular levels: difference in and influence of purification media.
We checked the effect that purification through different media can produce on rat mast cell response, considering different populations of pleural and peritoneal mast cells. We compared the media Ficoll, Percoll, bovine serum albumin and sucrose with the seldom used Nycodenz. Histamine release was elicited with compound 48/80, polymyxin B, ionophore A23187 and calcium on cells preincubated with FNa. High osmolarity media induced the most important changes on the cell response as compared to low osmolarity media. Our results show that Ficoll and sucrose induced the most notable changes in the response. Also, the more sensitive stimulus to the purification procedure is sodium fluoride-calcium. The response to ionophore A23187 did not show any important alteration.
Departamento de Farmacología, Facultad de Veterinaria, Lugo, España.
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D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D005362:Ficoll / Q000494:pharmacology; D007472:Iohexol / Q000494:pharmacology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology*; D010994:Pleura / Q000166:cytology*; D011112:Polymyxin B / Q000494:pharmacology; D011205:Povidone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012822:Silicon Dioxide / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D003470:Culture Media; D005362:Ficoll; D012710:Serum Albumin, Bovine; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D007472:Iohexol; D013395:Sucrose; C016039:Percoll; D012822:Silicon Dioxide; D012969:Sodium Fluoride; D011205:Povidone; D011112:Polymyxin B; D002118:Calcium