Transformation with katG restores isoniazid-sensitivity in Mycobacterium tuberculosis isolates resistant to a range of drug concentrations.
Isoniazid-resistant isolates of Mycobacterium tuberculosis were transformed with a plasmid vector carrying the functional catalase-peroxidase (katG) gene. Expression of katG restored full drug susceptibility in isolates initially resistant to concentrations ranging from 3.2 to > 50 micrograms ml-1. Transformation with the corresponding katG gene from Escherichia coli resulted in low-level expression of catalase and peroxidase activities and conferred partial isoniazid sensitivity.
MRC Tuberculosis and Related Infections Unit, Hammersmith Hospital, London, UK.
Y
Y
Zhang
T
T
Garbe
D
D
Young
D001426:Bacterial Proteins; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D007538:Isoniazid / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000378:metabolism; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism*; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D010544:Peroxidases; D002374:Catalase; C076136:catalase HPI; D009195:Peroxidase; D007538:Isoniazid