Protein phosphatase 2A alpha involvement in granulocytic differentiation of HL-60 cells.
Protein phosphatase 2A alpha (PP-2A alpha) in one of the catalytic subunits of PP-2A, which is composed of catalytic and regulatory subunits. In fission yeasts, PP-2A mutants cause mitotic defects and strikingly different cell cycle phenotypes. In rat hepatocellular carcinomas, mRNA expression of PP-2A alpha was increased compared to that of normal hepatocytes. From these studies, PP-2A alpha has been thought to be involved in proliferation, and we expected that PP-2A alpha would decrease in HL-60 cells on dimethyl sulfoxide(DMSO)-induced granulocytic differentiation with decreased growth rate. In this study, we show that PP-2A alpha expression increases in HL-60 cells on DMSO-induced granulocytic differentiation, while PP-1 gamma, which is one of catalytic subunits of protein phosphatase 1, expression did not change significantly. These results suggest that PP-2A alpha may be involved in regulation of differentiation of hematopoietic cells.
Sasaki
K
K
First Department of Internal Medicine, Kagawa Medical School, Japan.
Ikeda
K
K
Nagai
M
M
Shima
H
H
Nagao
M
M
Takahara
J
J
Irino
S
S
D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology* / Q000473:pathology*; D046911:Macromolecular Substances; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
D046911:Macromolecular Substances; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D004121:Dimethyl Sulfoxide