Nigerythrin and rubrerythrin from Desulfovibrio vulgaris each contain two mononuclear iron centers and two dinuclear iron clusters.
The trivial name 'rubr-erythrin' is a contraction of two other trivial names: rubredoxin (ruber, red) and hemerythrin. It names a protein of undetermined biological function which putatively carries rubredoxin-like mononuclear iron and hemerythrin-like dinuclear iron. The name 'nigerythrin' (niger, black) is an analogy of rubrerythrin. It identifies a second protein of undetermined function which has prosthetic groups similar to rubrerythrin. Rubrerythrin was initially described [LeGall, J., Prickril, B. C., Moura, I., Xavier, A. V., Moura, J. J. G. & Huynh, B.-H. (1988) Biochemistry 27, 1636-1642] as a homodimer with four iron ions arranged into two rubredoxin sites and one inter-subunit dinuclear cluster. Nigerythrin is a novel protein. Here, we report that both proteins are homodimers, each dimer carrying not four but six iron ions in two mononuclear centers and two dinuclear clusters. Rubrerythrin and nigerythrin are probably both located in the cytoplasm; they are differentially characterized with respect to molecular mass, pI, N-terminal sequence, antibody cross-reactivity, optical absorption, EPR spectroscopy, and reduction potentials. All three reduction potentials in both proteins are > +200 mV. These appear too high to be of practical relevance in the cytoplasm of the sulfate reducer Desulfovibrio vulgaris (Hildenborough). We suggest the possibility of a non-redox role for both proteins with all six iron ions in the ferrous state.
Department of Biochemistry, Wageningen Agricultural University, The Netherlands.
A J
AJ
Pierik
R B
RB
Wolbert
G L
GL
Portier
M F
MF
Verhagen
W R
WR
Hagen
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D016969:Desulfovibrio vulgaris / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D006422:Hemerythrin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D007501:Iron / Q000032:analysis*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012416:Rubredoxins; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D005288:Ferredoxins; D006422:Hemerythrin; D012416:Rubredoxins; C079962:nigerythrin protein, Desulfovibrio vulgaris; C055857:rubrerythrins; D007501:Iron