Antibody responses to the env epitopes of human T-lymphotropic virus type I in rhesus macaques' naturally infected with simian T-lymphotropic virus type I.
Synthetic peptides derived from the env protein of human T-lymphotropic virus type I (HTLV-I) were used to identify the immunodominant motifs in rhesus macaques naturally infected with simian T-lymphotropic virus type I (STLV-I). Of the 13 peptides derived from the env protein of HTLV-I, Env-1(191-214) and Env-5(242-257) reacted with 81% (44/54) and 54% (29/54) of specimens from infected monkeys, respectively. A recombinant protein (MTA-I162-209) reacted with 53 of 54 (98%) STLV-I-infected serum specimens. While similar immune reactivities to Env-1 and MTA-1 were observed in both HTLV-I-infected human sera and STLV-I-infected monkey sera, the reactivity to Env-5 was significantly higher in HTLV-I-infected human sera. Differential reactivities may be attributed to the differences in the secondary structures of the STLV-I and HTLV-I envelope protein in the Env-5 region, since homologous peptide with STLV-I sequence did not result in enhanced sensitivity of anti-Env-5 antibody detection.
Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, GA 30333.
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Rudolph
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Yee
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Palker
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Coligan
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Lal
D000595:Amino Acid Sequence; D000818:Animals; D015686:Gene Products, env / Q000737:chemistry / Q000276:immunology*; D015481:HTLV-I Antibodies / Q000096:biosynthesis*; D015486:HTLV-I Antigens / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000276:immunology*; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D012192:Retroviridae Infections / Q000276:immunology* / Q000382:microbiology; D015682:Retroviridae Proteins, Oncogenic / Q000737:chemistry / Q000276:immunology; D015303:Simian T-lymphotropic virus 1 / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus
D015686:Gene Products, env; D015481:HTLV-I Antibodies; D015486:HTLV-I Antigens; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D015682:Retroviridae Proteins, Oncogenic; D054299:env Gene Products, Human Immunodeficiency Virus; C055265:gp21 protein, Human T-lymphotropic virus 1; C055266:gp46 protein, Human T-cell leukemia virus type I