[Transfer of dominant selective genes using retroviral vectors--a simple and quick method for obtaining hybridoma hybrids].
Two different techniques were used for labeling cells for subsequent generation of hybrid hybridomata. One of them was described earlier by De Lau and included isolation of an HATSNeoR double mutants of hybridomata cells. We proposed the second one consisting of introduction of dominant selectable genes conferring resistance to genecetin (G418) and hygromycin B into distinct hybridomata by retroviral vectors. Hybrid hybridomata secreting the bispecific antibodies have been obtained by both methods. However, comparison of the methods has revealed that highly efficient retroviral gene transfer of the selectable genes is a simpler and more convenient method for generation of cellular hybrids.
Fomin
I K
IK
Tikhonov
I I
II
Prasolov
V S
VS
Voĭtenok
N N
NN
D016475:3T3 Cells; D000818:Animals; D004352:Drug Resistance, Microbial / Q000235:genetics; D005799:Genes, Dominant; D005822:Genetic Vectors; D006825:Hybridomas; D043564:Inorganic Pyrophosphatase; D051379:Mice; D011755:Pyrophosphatases / Q000276:immunology; D012190:Retroviridae / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D014409:Tumor Necrosis Factor-alpha; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase