A new method for rapidly removing contaminating micro-organisms from porcine parvovirus or pseudorabies virus master-seed suspensions.
Virus-contaminated cell cultures are a major problem in the bio-industry. Methods employed to date to remove contaminating micro-organisms are slow and costly, and a new method is proposed here which is simple and rapid. The method uses polyacrylamide beads coated with specific antibodies which yielded bead-antibody-virus complexes when suspended in the virus solution to be cleared. The purified virus was propagated in cells which show phagocytic activity. Vaccine master-seed virus is shown to be rapidly cleaned and propagated using this method.
Rivera
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E
National Veterinary Institute, Division of Vaccine Research, Uppsala, Sweden.
Grönvik
K O
KO
Karlsson
K A
KA
D000818:Animals; D000914:Antibodies, Viral; D002417:Cattle; D002478:Cells, Cultured; D004340:Drug Contamination; D011558:Herpesvirus 1, Suid / Q000276:immunology / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D010321:Parvoviridae / Q000276:immunology / Q000302:isolation & purification*; D010586:Phagocytes / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure; D013552:Swine; D014765:Viral Vaccines / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods
D000914:Antibodies, Viral; D014765:Viral Vaccines