Molecular characterization of a murine homologue of alpha 2C4 adrenoceptor subtype gene.
A clone MA2C4 encoding the murine homologue of adrenoceptor alpha 2C4 was identified, amplified and purified from a mouse genomic library (EMBL-3). The Pstl-EcoRl digested fragment (2409 base pairs) of the clone contains the nucleotide sequence encoding a putative receptor protein (458 amino acids), which is highly homologous in the transmembrane and G protein-coupling domains to the human and rat isoceptors. The 5' untranslated region (0.4kb) is characterized by high GC content and CpG island count whereas a polyadenylation signal (AATAAA) is located 481 base pairs downstream from the termination codon in the 3' untranslated region (0.6kb).
Institute of Clinical Medicine, National Yang-Ming Medical College, Taipei, Taiwan, ROC.
Y H
YH
Chang
N C
NC
Chang
W M
WM
Chen
A C
AC
Chang
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016678:Genome; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D008565:Membrane Proteins; D011942:Receptors, Adrenergic, alpha