[Preparation of phenylpropylsilica and its application to high performance liquid chromatographic analysis of proscillaridin].
Proscillaridin in commercial tablet preparations was analyzed by HPLC on a phenylpropyl chemically bonded stationary phase, prepared by treating porous silica with phenylpropyldimethylchlorosilane (PHP). From the elemental analysis data for carbon, the number of bonded PHP surface groups per 1 nm2 of silica (mean pore diameter 13.1 nm, specific surface area 330 m2/g, mean particle size 5.5 microns) in PHP gel was calculated to be 2.07. Using methanol-distilled water (80:20, v/v) as an eluent, the separation of proscillaridin was successfully performed on the PHP column.
Gifu Prefectural Tajimi Hospital, Japan.
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Okamoto
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Tsukahara
D002851:Chromatography, High Pressure Liquid; D005782:Gels; D010316:Particle Size; D010622:Phencyclidine / Q000031:analogs & derivatives; D011442:Proscillaridin / Q000032:analysis*; D012822:Silicon Dioxide; D013607:Tablets
D005782:Gels; D013607:Tablets; C020925:1-(1-phenylcyclohexyl)pyrrolidine; D012822:Silicon Dioxide; D010622:Phencyclidine; D011442:Proscillaridin