Production of interleukin-1, interleukin-6, and tumor necrosis factor alpha in the uterus of pseudopregnant mice.
Pseudopregnant mice were produced by mating normal cycling females with a vasectomized male. Interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF alpha) mRNA and bioactivity were detected between Days 1 and 8 in untreated pseudopregnant mice and during oil-induced decidualization. High concentrations of IL-1, IL-6, and TNF alpha mRNA and bioactivity were detected in the uterus within 12 h after mating, and both mRNA and bioactivity decreased progressively through Day 3. Peaks of IL-1, IL-6, and TNF alpha bioactivity were detected between Days 4 and 8. Oil-induced decidualization was associated with increased and prolonged production of IL-1 and IL-6 and decreased production of TNF alpha relative to these values in uninjected controls. The dynamics of cytokine production in pseudopregnant mice were similar to those previously observed in pregnant mice.
Department of Pathology and Oncology, University of Kansas Medical Center Kansas City, 66160-7410.
R
R
Choudhuri
G W
GW
Wood
D000818:Animals; D003656:Decidua / Q000187:drug effects / Q000502:physiology; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008297:Male; D051379:Mice; D011555:Pseudopregnancy / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012715:Sesame Oil / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D014599:Uterus / Q000378:metabolism*
D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D012715:Sesame Oil