Failure to verify high levels of pyrroloquinoline quinone in eggs and skim milk.
The levels of pyrroloquinoline quinone (PQQ) in eggs, skim milk and milk was re-examined by the use of gas chromatography/mass spectrometry. PQQ was extracted from the samples, after addition of [U-13C]PQQ as internal standard, with n-butanol and Sep-Pak C18 cartridges. After derivatization of PQQ with phenyltrimethylammonium hydroxide, molecular peaks at m/z 448 and 462 were used for detection of PQQ and [U-13C]PQQ, respectively, by selected ion monitoring. PQQ could be detected in eggs, skim milk and milk, but their levels were very low (e.g., egg yolk: 7.0 ng/ml; skim milk: 2.5 ng/g dry weight). Our data for eggs and skim milk are far below those measured by the redox cycling method of Gallop's group.
Kumazawa
T
T
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
Seno
H
H
Suzuki
O
O
D000818:Animals; D003067:Coenzymes / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D008892:Milk / Q000737:chemistry*; D045542:PQQ Cofactor; D011200:Poultry; D015363:Quinolones / Q000032:analysis*
D003067:Coenzymes; D015363:Quinolones; D045542:PQQ Cofactor