A partial physical map for the chromosome of alkalophilic Bacillus sp. strain C-125.
Bacillus sp. strain C-125 has been chosen as a model alkalophilic bacterium to understand how adaptation to growth at high pH is achieved. To aid genetic analysis, we have started characterization of its genome. By using the two infrequently-cutting restriction endonucleases, AscI and Sse8387I, in conjunction with pulsed-field electrophoretic techniques, the size of the genome was found to be 3.7 Mb. Southern blot analysis of single, double and partial digests of Bacillus sp. strain C-125 DNA, using AscI-linking clones, gene probes and purified Bacillus sp. strain C-125 restriction fragments, allowed a putative chromosome map to be constructed.
Sutherland
K J
KJ
RIKEN Institute, Saitama, Japan.
Hashimoto
M
M
Kudo
T
T
Horikoshi
K
K
D000222:Adaptation, Physiological; D001407:Bacillus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D015183:Restriction Mapping
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes