Effects of the benzothiadiazine TAG on channel activation at mammalian glycine receptors.
The effects of the benzothiadiazine TAG on membrane channels activated by taurine and glycine were investigated in dissociated spinal cord neurons from embryonic mice. TAG (100-200 microM) dose-dependently and reversibly antagonized the ability of glycine and taurine to activate chloride permeable ionic channels in these patches. This effect of TAG was due to shortening of the mean open time of the glycine and taurine activated channels. In addition, TAG significantly increased the mean shut time of taurine activated channels. The mean amplitude single channel currents activated by either agonist was unaltered by TAG. Single channel currents activated by taurine were significantly more sensitive to the blocking action of TAG than currents activated by glycine.
Mathers
D A
DA
Department of Physiology, University of British Columbia, Vancouver, Canada.
D000818:Animals; D001581:Benzothiadiazines / Q000494:pharmacology*; D018118:Chloride Channels; D004594:Electrophysiology; D005998:Glycine / Q000494:pharmacology; D008565:Membrane Proteins / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000378:metabolism; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology
D001581:Benzothiadiazines; D018118:Chloride Channels; D008565:Membrane Proteins; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D013654:Taurine; C032946:6-aminomethyl-3-methyl-4H-1,2,4-benzothiadiazine 1,1-dioxide; D005998:Glycine