Cloning and functional expression of a human parathyroid hormone receptor.
We have cloned a human receptor for parathyroid hormone from a kidney complementary DNA library. The deduced sequence of 593 amino acids shows high homology to the previously cloned receptors from opossum and rat. Expressed in COS-1 cells, the human receptor binds to parathyroid hormone-(1-38) with high affinity (pKD = 8.5) and is functionally coupled to adenylate cyclase (pEC50 = 9.4). At high concentrations of agonist, the receptor also activates phosphoinositide turnover.
Sandoz Pharma Ltd, Preclinical Research, Basel, Switzerland.
H
H
Schneider
J H
JH
Feyen
K
K
Seuwen
N R
NR
Movva
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007668:Kidney / Q000201:enzymology / Q000378:metabolism; D008969:Molecular Sequence Data; D010281:Parathyroid Hormone / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018016:Receptors, Parathyroid Hormone
D010281:Parathyroid Hormone; D011956:Receptors, Cell Surface; D018016:Receptors, Parathyroid Hormone; D004247:DNA; D000262:Adenylyl Cyclases