Investigation of premalignant lesions of larynx epithelium with histoautoradiography and HPV-assay.
The histological evaluation of precancerous alterations of larynx epithelium is, in transitional cases, very difficult. The differentiation of harmless metaplasia, hyperplasia and keratosis from abnormal proliferation depends on the pathologist's training and experience. Fine diagnostic lines separate the different grades of dysplastic epithelial alterations. The tritiated thymidine, 3H-TdR, labelling and histoautoradiographic procedure of biopsy specimens taken from the larynx provide the possibility to recognise the DNA synthetiser cells. The number, location and distribution of 3H-TdR labelled cells in the epithelial layers help us to distinguish with clarity the growth activity, the degree of dysplasia. After 3 weeks' exposition time of sections prepared by photoemulsion and stained with haematoxilin-eosin, the result can be assessed. In addition to histoautoradiography HPV-assay was applied, as HPV may play a role in the pathogenesis of laryngeal preneoplastic alterations.
Department of Otolaryngology and Pathology, Uzsoki District Hospital, Budapest, Hungary.
J
J
Elö
Z S
ZS
Balatoni
L
L
Varga
A
A
Bajtai
J
J
Hídvégi
D001345:Autoradiography; D001706:Biopsy; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000382:microbiology* / Q000473:pathology*; D007830:Larynx / Q000382:microbiology / Q000473:pathology*; D027383:Papillomaviridae / Q000302:isolation & purification*; D011230:Precancerous Conditions / Q000382:microbiology* / Q000473:pathology*; D014412:Tumor Virus Infections / Q000175:diagnosis*
D015344:DNA Probes, HPV; D004279:DNA, Viral