A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions.
Transcription of the Saccharomyces cerevisiae CTT1 gene encoding the cytosolic catalase T is activated by a variety of stress conditions: it is derepressed by nitrogen starvation and induced by heat shock. Furthermore, it is activated by osmotic and oxidative stress. This study shows that a CTT1 upstream region previously found to be involved in nitrogen, cAMP and heat control (base pairs -382 to -325) contains a UAS element (STRE, -368 to -356), which is sufficient for the activation of a reporter gene by all types of stress acting on CTT1. Gel retardation experiments demonstrated the existence of a factor specifically binding to STRE, but to a lesser extent to mutated elements having partly or entirely lost the ability to mediate stress control. Heat activation of STRE, but not of a canonical heat shock element, is enhanced by a ras2 defect mutation, which enhances thermotolerance, and is dramatically reduced by a bcy1 disruption mutation, which decreases thermotolerance. It can be hypothesized, therefore, that the novel stress control element is important for the establishment of induced stress tolerance.
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Austria.
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Marchler
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Schüller
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Adam
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Ruis
D001483:Base Sequence; D002374:Catalase / Q000235:genetics*; D000242:Cyclic AMP / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D009995:Osmosis; D010084:Oxidation-Reduction; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D004271:DNA, Fungal; D005656:Fungal Proteins; D000242:Cyclic AMP; D002374:Catalase; D011494:Protein Kinases; D009584:Nitrogen