Coamplification of the hst and bcl-1 oncogenes in advanced squamous cell carcinomas of the head and neck.
Squamous cell carcinomas of the head and neck from 40 untreated patients were analysed for rearranged or amplified proto-oncogenes by Southern blot hybridisation. The bcl-1 and the hst genes were coamplified 8-32-fold in 5 patients (12.5%). Only males with stage III and IV disease showed coamplification of these oncogenes. Northern blot analysis of the positive samples did not show expression of bcl-1 or hst genes. In contrast, a third oncogene located on chromosome 11 (Ha-ras-1) was not amplified in these tumours. Disease development was observed in all cases over a minimum period of 3 years. Survival of the patients with coamplification of hst/bcl-1 seemed to be shorter than of those with stage III and IV disease without amplification. This difference was not significant probably due to the small number of investigated patients.
Volling
P
P
Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde Universität Köln, F.R.G.
Jungehülsing
M
M
Jücker
M
M
Stützer
H
H
Diehl
V
V
Tesch
H
H
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D015321:Gene Rearrangement; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011519:Proto-Oncogenes; D012907:Smoking
D004273:DNA, Neoplasm