Effect of exogenous hydrogen peroxide on iodide transport and iodine organification in FRTL-5 rat thyroid cells.
Hydrogen peroxide plays an important role in the regulation of iodination and thyroid hormone formation. In the present study, the effect of exogenous H2O2 on 125I transport and organification was investigated in FRTL-5 rat thyroid cells. Less than 20 passages after subcloning, cells in 24-well plates (6 x 10(4) cells/well) were maintained in a thyrotropin (TSH)-containing medium (6H) for 3 days. A TSH-free medium (5H) was then used for the next 7 days. A 1-h exposure to H2O2 stimulated 125I transport and 125I organification at 0.1-0.5 mmol/l H2O2 and had a toxic effect on FRTL-5 cell at 5 mmol/l. Hydrogen peroxide (0.5 mmol/l) augmented the iodide transport and iodine organification induced by TSH (333 U/l) by two- and threefold, respectively. The biphasic effect of H2O2 was blocked totally by 5-200 micrograms/l of catalase. Catalase by itself did not influence TSH-mediated 125I transport and 125I organification. Hydrogen peroxide (0.5 mmol/l) added to cells in 5H medium increased Na+K(+)-ATPase activity twofold. Ouabain (1 mmol/l), an inhibitor of Na+K(+)-ATPase, completely inhibited the twofold increase in 125I transport induced by 0.5 mmol/l H2O2 but only inhibited H2O2-induced 125I organification by 28%. Methimazole (1 mmol/l), an inhibitor of thyroid peroxidase, had no effect on H2O2-mediated 125I transport but totally blocked the fivefold rise in 125I organification induced by 0.5 mmol/l H2O2. The effect of H2O2 on intracellular cyclic adenosine monophosphate (cAMP) levels also was studied.(ABSTRACT TRUNCATED AT 250 WORDS)
Chen
G
G
Edocrine Research Laboratory, West Los Angeles VA Medical Center 90073.
Pekary
A E
AE
Sugawara
M
M
Hershman
J M
JM
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D004357:Drug Synergism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000378:metabolism; D007454:Iodides / Q000493:pharmacokinetics*; D007455:Iodine / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology
D007454:Iodides; D010042:Ouabain; D013972:Thyrotropin; D007455:Iodine; D006861:Hydrogen Peroxide; D000242:Cyclic AMP; D000254:Sodium-Potassium-Exchanging ATPase