Cytochromes c1 of kinetoplastid protozoa lack mitochondrial targeting presequences.
We have used the polymerase chain reaction to amplify cDNA fragments that encode the amino-terminal sequences of cytochrome c1 from two distantly related kinetoplastid species, Crithidia fasciculata and Bodo caudatus. Cloning and sequencing of these fragments have revealed that these proteins lack conventional mitochondrial targeting presequences.
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham 35294.
J W
JW
Priest
Z A
ZA
Wood
S L
SL
Hajduk
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016831:Crithidia fasciculata / Q000201:enzymology; D003575:Cytochromes c1 / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D056890:Eukaryota / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004247:DNA; D003575:Cytochromes c1