Immunocytochemical localization of alpha-, beta I-, beta II- and gamma-subspecies of protein kinase C in the motor and premotor cortices of the rhesus monkey.
We obtained evidence for the localization of alpha-, beta I-, beta II- and gamma-subspecies of protein kinase C (PKC) in the monkey motor and premotor cortices (Brodmann's areas 4 and 6). In Brodmann's area 4, the immunoreactivity for the alpha-PKC was present in horizontal and round cells in the layers I and II, and small pyramidal cells in layer III and also in the glial cells in subcortical white matter. The alpha-PKC immunopositive glial cells contained GFAP-immunoreactive product. The beta I-PKC immunoreactivity was present in the round cells in layer I and in the pyramidal cells in the layer V, including Betz cells. The beta II-PKC immunoreactivity was observed as small dots in perikarya of the small and medium-sized pyramidal cells in layers II, III, V and VI, but not in layer I. The gamma-PKC immunoreactive cell bodies were observed in layers II, III and VI, and most of the immunoreactive cells were pyramidal. Intense gamma-PKC immunoreactivity was found in the neuropils of layers I and II. Similar distributions of four PKC subspecies were seen in Brodmann's area 6, except that beta I-PKC immunoreactive Betz cells were not present. The unique localization of PKC subspecies suggested that each PKC subspecies was involved in the specific function in motor and premotor cortices of the rhesus monkey.
Department of Pharmacology, Kobe University School of Medicine, Japan.
S
S
Tominaga
N
N
Saito
T
T
Tsujino
C
C
Tanaka
D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D008253:Macaca mulatta; D008297:Male; D009044:Motor Cortex / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D014018:Tissue Distribution
D007527:Isoenzymes; D011493:Protein Kinase C