Seminal fluid analysis and fine-needle aspiration cytology in the diagnosis of carcinoma in situ of the testis.
Surgical biopsies and fine-needle aspirates of peri-tumoural seminiferous tubules were taken from freshly-excised orchidectomy specimens. In addition, patients with suspected germ cell tumour provided a peri-operative sample of seminal fluid. All three tissue preparations were investigated using flow cytometry, immunochemistry for placental-like alkaline phosphatase and enzymochemistry for alkaline phosphatase. Biopsy and fine-needle aspiration cytology provide the greatest diagnostic accuracy for carcinoma-in-situ using these techniques. Seminal fluid analysis did not provide a satisfactory diagnostic yield in the series of patients presented. A seminal plasma placental-like alkaline phosphatase immunoassay failed to discriminate CIS because of the high level of background germ cell alkaline phosphatase.
Department of Urology, Western General Hospital, Edinburgh, UK.
E T
ET
Brackenbury
T B
TB
Hargreave
G C
GC
Howard
M A
MA
McIntyre
D000293:Adolescent; D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D001707:Biopsy, Needle; D002278:Carcinoma in Situ / Q000175:diagnosis*; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009373:Neoplasms, Germ Cell and Embryonal / Q000175:diagnosis*; D011003:Ploidies; D012661:Semen / Q000737:chemistry*; D013736:Testicular Neoplasms / Q000175:diagnosis*
D004273:DNA, Neoplasm; D000469:Alkaline Phosphatase