Expression of mRNAs encoding the alpha 1 and the beta 1 subunits of Na+, K(+)-ATPase in human lymphocytes activated with phytohaemagglutinine.
Increase in Na+, K(+)-ATPase mRNAs was detected in activated lymphocytes by the RT-PCR method. alpha 1 subunit mRNA gradually increased with time and by 36 h was 2.4 times higher than at the start. Increase in the beta 1 mRNA was transient reaching a maximum in the 8 h probe and declining to the initial level in the 24 and 36 h probes. The elevation of Na+, K(+)-ATPase mRNAs does not underlie a cycloheximide-inhibited increase in cation pumping peculiar to the prereplicative period as can be judged from the fact that Act D fails to eliminate PHA-induced enhancement of pump fluxes.
Vereninov
A A
AA
Institute of Cytology, Academy of Sciences, St. Petersburg, Russian Federation.
Marakhova
I I
II
Osipov
V V
VV
Toropova
F V
FV
D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics*; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism
D010835:Phytohemagglutinins; D012333:RNA, Messenger; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase