pH method for determination of cholinesterase in whole blood: collaborative study.
A collaborative study of an indirect method for determination of cholinesterase activity was conducted with cattle blood. Ten collaborators analyzed 8 samples of varying degrees of cholinesterase inhibition. The sample is diluted with a buffer, and the pH change that results from the hydrolysis of acetylcholine is monitored. Repeatability relative standard deviations (RSDr) ranged from 6.6 to 8.6%, and reproducibility relative standard deviations (RSDR) ranged from 10.7 to 17.3% for mean activities of 0.60-0.40 delta pH/h. All samples that were completely inhibited were detected. The method was adopted first action by AOAC International for determination of cholinesterase activity in whole bovine blood.
Imerman
P M
PM
Iowa State University, Veterinary Diagnostic Laboratory, Ames 50010.
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood; D002417:Cattle; D002802:Cholinesterases / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D015203:Reproducibility of Results
D007202:Indicators and Reagents; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D000109:Acetylcholine