[The effect of NAD on binding and liberation of (14)C-GABA during convulsant administration].
Administration of corazol into animals led to a decrease in content of NAD and gamma-aminobutyric acid (GABA) in brain. Under these conditions, binding of 14C-GABA was increased and its liberation was inhibited in the synaptosomes of the brain cortex. Additional administration of nicotinamide, accompanied by considerable increase in content of NAD and GABA, caused a decrease in accumulation of exogenous GABA in the synaptosomes and removed the effects produced by the convulsant agent. Kinetics of 14C-GABA binding in the presence of NAD demonstrated that the more effective inhibition of the binding occurred in the animals treated with the convulsant drug. NAD appears to affect the GABA-ergic transmission at the postsynaptic level.
A I
AI
Fomenko
S P
SP
Stepanenko
P K
PK
Parkhomets
G V
GV
Donchenko
D000818:Animals; D002247:Carbon Isotopes; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009243:NAD / Q000378:metabolism / Q000494:pharmacology*; D010433:Pentylenetetrazole / Q000008:administration & dosage*; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002247:Carbon Isotopes; D009243:NAD; D005680:gamma-Aminobutyric Acid; D010433:Pentylenetetrazole