Preparation of peptide-carrying microspheres with bioactivity on platelets.
A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.
Faculty of Science and Technology, Keio University, Yokohama 223, Japan.
Y
Y
Kasuya
K
K
Fujimoto
M
M
Miyamoto
T
T
Juji
A
A
Otaka
S
S
Funakoshi
N
N
Fujii
H
H
Kawaguchi
D000178:Acrylamides; D000255:Adenosine Triphosphate / Q000097:blood; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D004337:Drug Carriers; D006801:Humans; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009842:Oligopeptides / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011137:Polystyrenes
D000178:Acrylamides; D001672:Biocompatible Materials; D004337:Drug Carriers; D007202:Indicators and Reagents; D009842:Oligopeptides; D010975:Platelet Aggregation Inhibitors; D011137:Polystyrenes; D000255:Adenosine Triphosphate; C046617:arginyl-glycyl-aspartyl-serine