Mechanisms involved in the adaptations of the adipocyte adrenergic signal-transduction system and their modulation by growth hormone during the lactation cycle in the rat.
The mechanisms responsible for the diminished lipolytic response of adipocytes to catecholamines after litter removal from lactating rats and their modulation by growth hormone have been investigated. Lactation, litter removal and growth-hormone treatment did not alter the ability of noradrenaline to activate protein kinase A (A-kinase), showing that the defect in signal transduction in rats after litter removal is after A-kinase. Litter removal had no effect on hormone-sensitive lipase activity itself, but the proportion of the lipase associated with the fat droplet was decreased; growth-hormone treatment increased hormone-sensitive lipase activity and the proportion associated with the fat droplet. In addition, a number of other adaptations in the beta-adrenergic signal-transduction system occur during the lactation cycle and in response to growth hormone treatment, including changes in receptor number, adenylate cyclase activity and cyclic AMP phosphodiesterase activity, but a defect in the ability of hormone-sensitive lipase to associate with the lipid droplet appears to be the major reason for the diminished response to catecholamines on litter removal.
Hannah Research Institute, Ayr, Scotland, U.K.
R G
RG
Vernon
L
L
Piperova
P W
PW
Watt
E
E
Finley
S
S
Lindsay-Watt
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000220:Adaptation, Biological; D000262:Adenylyl Cyclases / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D004655:Emulsions / Q000378:metabolism; D004789:Enzyme Activation; D004988:Ethers, Cyclic / Q000494:pharmacology; D005260:Female; D005990:Glycerol / Q000378:metabolism; D013006:Growth Hormone / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007545:Isoproterenol / Q000494:pharmacology; D007774:Lactation / Q000502:physiology*; D050356:Lipid Metabolism; D009638:Norepinephrine / Q000494:pharmacology; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D015398:Signal Transduction; D002787:Sterol Esterase / Q000378:metabolism; D014886:Weaning
D004655:Emulsions; D004988:Ethers, Cyclic; D011943:Receptors, Adrenergic, beta; D019319:Okadaic Acid; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D011494:Protein Kinases; D002787:Sterol Esterase; D010749:Phosphoprotein Phosphatases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000262:Adenylyl Cyclases; D007545:Isoproterenol; D005990:Glycerol; D009638:Norepinephrine