Toxicity testing using the isolated in vitro perfused ovary.
This article discusses the use of in vitro perfusion techniques as a tool for toxicity testing in the ovary and how the rat ovary has been adapted for this purpose. A brief review of the development of in vitro ovarian perfusion is provided, focusing on steroidogenesis and physiology of ovulation. Adaptation of this model for use as a toxicologic model is discussed in the context of other isolated organ models, (that is, liver, heart, lung). Surgical procedures, perfusate and criteria for viability are outlined. Advantages of this technique are highlighted including ability to administer high doses of drugs directly to intact organ devoid of other influences. Applications of this model are discussed and data from studies of glutathione depleted ovaries perfused with hexachlorobenzene (HCB) are presented. Increased oxygen consumption after addition of HCB is suggestive of a disordered respiratory metabolism and is an example of future markers of ovarian injury using this innovative technique.
Department of Obstetrics and Gynaecology, University of Calgary, Alberta, Canada.
J F
JF
Jarrell
M L
ML
Sevcik
D C
DC
Villeneuve
P O
PO
Janson
D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D066298:In Vitro Techniques; D008954:Models, Biological; D010053:Ovary / Q000187:drug effects* / Q000502:physiology; D010477:Perfusion; D051381:Rats; D014116:Toxicology / Q000379:methods*