The encephalomyocarditis virus 3C protease is rapidly degraded by an ATP-dependent proteolytic system in reticulocyte lysate.
The encephalomyocarditis virus 3C protease has been observed to undergo rapid degradation, both in vivo in mouse cells and in vitro in reticulocyte lysate. Experiments were carried out to characterize the turnover of the 3C protease in reticulocyte lysate. 3C protease prepared in reticulocyte lysate by in vitro translation and processing of a precursor polyprotein could be separated from the proteolytic activity responsible for its degradation. This implies the 3C protease is not directly involved in its own proteolysis. Active 3C protease flanked by only a few amino acids was degraded at a rate identical to that of a similar protein containing an inactivated catalytic site. This indicates that 3C protease activity is not indirectly required for the proteolytic process. Other viral proteins, including the 3D polymerase and capsid proteins, were relatively stable in the lysate. In addition, polyprotein precursors containing 3C protease with an inactive catalytic site and various flanking proteins displayed distinctly different stabilities. These results suggest that the reticulocyte proteolytic system functions in a selective manner toward the viral proteins. The effects of several proteolytic inhibitors on the lysate proteolytic system were evaluated. The results of these experiments indicate that the rapid degradation of the EMC virus 3C protease requires the hydrolysis of ATP.
Oberst
M D
MD
Department of Chemistry, Bates College, Lewiston, Maine 04240.
Gollan
T J
TJ
Gupta
M
M
Peura
S R
SR
Zydlewski
J D
JD
Sudarsanan
P
P
Lawson
T G
TG
D000086042:3C Viral Proteases; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004686:Encephalomyocarditis virus / Q000201:enzymology*; D010450:Endopeptidases / Q000378:metabolism*; D066298:In Vitro Techniques; D011480:Protease Inhibitors / Q000494:pharmacology; D014176:Protein Biosynthesis; D012156:Reticulocytes / Q000378:metabolism*; D014764:Viral Proteins
D011480:Protease Inhibitors; D014764:Viral Proteins; D000255:Adenosine Triphosphate; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases