Human lymphocyte sodium-hydrogen exchange. The influences of lipids, membrane fluidity, and insulin.
The relation between serum lipids, membrane fluidity, insulin, and the activity of the sodium-hydrogen exchanger was investigated in human lymphocytes from 83 subjects. Subjects had a wide range of serum lipids and no concurrent disease. Lymphocyte membrane anisotropy (inversely related to membrane fluidity) was measured with a fluorescence polarization method. Sodium-hydrogen exchange maximal proton efflux rate, affinity for external sodium, and resting pH were determined with the intracellular pH-sensitive fluorochrome 2',5'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Sodium-hydrogen exchange maximal proton efflux rate was negatively correlated with the age of the subject (p = 0.03). Membrane anisotropy correlated with serum triglyceride (p = 0.04). Multiple regression analysis demonstrated that the maximal proton efflux rate in human lymphocytes was significantly related to age (p = 0.005), systolic blood pressure (p = 0.04), membrane anisotropy (p = 0.03), and serum cholesterol (p = 0.03). Incubation of lymphocytes with insulin failed to affect sodium-hydrogen exchange kinetics, intracellular buffering power, or resting intracellular pH. These results suggest that membrane-bound transport proteins may be influenced by serum lipids and the fluidity of the lipid membrane in which they are bound, but they are unlikely to be affected by insulin.
Carr
P
P
Renal Laboratory, St. Thomas' Hospital, UMDS, London.
Taub
N A
NA
Watts
G F
GF
Poston
L
L
D000328:Adult; D002352:Carrier Proteins / Q000097:blood*; D005260:Female; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008055:Lipids / Q000097:blood*; D008094:Lithium / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D011522:Protons; D012964:Sodium / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D007328:Insulin; D008055:Lipids; D011522:Protons; D017923:Sodium-Hydrogen Exchangers; D008094:Lithium; D012964:Sodium