Pulsed-field gel electrophoretic analysis of Campylobacter jejuni DNA for use in epidemiological studies.
The cleavage patterns of the genomic DNA of 42 clinical isolates of Campylobacter jejuni determined by pulsed-field gel electrophoresis (PFGE) were compared with their Lior and TCK serotypes. The fragment patterns of DNA obtained with SalI and SmaI restriction enzymes did not always accord with the corresponding serotypes but strains of the same serotype could be further divided into subtypes by their cleavage patterns. This PFGE method may prove useful for subclassifying C. jejuni.
Laboratory of Bacteriology, Aichi Prefectural Institute of Public Health, Japan.
Y
Y
Suzuki
M
M
Ishihara
M
M
Funabashi
R
R
Suzuki
S
S
Isomura
T
T
Yokochi
D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D012703:Serotyping / Q000379:methods*
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases