Effects of adrenal function tests on the levels of endogenous digitalis-like substances and some pituitary hormones.
In order to study the influence of the hypothalamic-pituitary-adrenal axis on the levels of endogenous digitalis-like substances (EDLS) in plasma and urine, eight healthy subjects (25-40 years old) were given dexamethasone 1 mg orally and tetracosactide (an ACTH analog) 0.25 mg i.v., on separate occasions. The circulating levels of EDLS, TSH, PRL and AVP following administration of either test drug, and under control conditions, were measured by a RIA for digoxin and specific RIAs for each hormone. Plasma cortisol was measured by liquid chromatography. The area under the curve (AUC) of hormone levels between 08.00 and 09.30 was used for data comparisons. Urine was collected before and after each test dose, and analysed for cortisol levels by gas chromatography/mass spectrometry, and for digitalis-like activity both by RIA and by a bioassay measuring 86Rb-uptake into red blood cells. Dexamethasone suppressed the AUC of plasma and urine levels of cortisol (p = 0.0001 and p < 0.01, respectively) and immunoreactive EDLS (p = 0.0007 and p < 0.01), as well as serum levels of TSH (p = 0.0002) and PRL (p = 0.001), but did not alter AVP levels. The biological digitalis-like activity in the urine measured by the 86Rb-uptake assay was decreased, but not to a statistically significant degree. ACTH increased the levels of cortisol in plasma (p = 0.0001) and urine (p < 0.01) and the immuno-reactive EDLS in plasma (p = 0.03), but not in urine. There were no effects of ACTH on TSH, PRL or AVP.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Clinical Pharmacology, Lund University Hospital, Sweden.
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Vinge
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Erfurth
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Lundin
D000328:Adult; D001127:Arginine Vasopressin / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism* / Q000652:urine; D002298:Cardenolides; D003366:Cosyntropin / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004077:Digoxin; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000652:urine; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010912:Pituitary-Adrenal Function Tests; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000378:metabolism; D011388:Prolactin / Q000097:blood; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013972:Thyrotropin / Q000097:blood; D013997:Time Factors; D014556:Urine
D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D001127:Arginine Vasopressin; D003366:Cosyntropin; D004077:Digoxin; D003907:Dexamethasone; D011388:Prolactin; D013972:Thyrotropin; D000254:Sodium-Potassium-Exchanging ATPase; D006854:Hydrocortisone