Accessory cholera enterotoxin (Ace), the third toxin of a Vibrio cholerae virulence cassette.
Vibrio cholerae causes the potentially lethal disease cholera through the elaboration of the intestinal secretogen cholera toxin. A second toxin of V. cholerae, Zot, decreases intestinal tissue resistance by modifying intercellular tight junctions. In this report, a third toxin of V. cholerae, Ace (accessory cholera enterotoxin), is described. Ace increases short-circuit current in Ussing chambers and causes fluid secretion in ligated rabbit ileal loops. The predicted protein sequence of Ace shows striking similarity to eukaryotic ion-transporting ATPases, including the product of the cystic fibrosis gene. The gene encoding Ace is located immediately upstream of the genes encoding Zot and cholera toxin. The ctx, zot, and ace genes, which are located on a dynamic sector of the chromosome, comprise a V. cholerae "virulence cassette."
Department of Medicine, University of Maryland School of Medicine, Baltimore.
M
M
Trucksis
J E
JE
Galen
J
J
Michalski
A
A
Fasano
J B
JB
Kaper
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002772:Cholera Toxin / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D007082:Ileum / Q000187:drug effects; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000187:drug effects; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D011817:Rabbits; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014734:Vibrio cholerae / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics*
C081530:Ace toxin; D004251:DNA Transposable Elements; D009838:Oligodeoxyribonucleotides; D002772:Cholera Toxin