[Effects of prostaglandin E1, E2 and F2 alpha on the changes in the adenylate cyclase activities of rabbit urinary tract tissues].
Effects of prostaglandin E1, E2 and F2 alpha (PGE1, PGE2 and PGF2 alpha) on the changes in the adenylate cyclase (AC) activities of rabbit urinary tract tissues were studied in order to clarify whether the PGs' actions are mediated by cAMP in the urinary tract. AC activities were measured by the method of Salomon et al. The three PGs studied were all found to increase the AC activities dose-dependently in the renal pelvis, ureter, bladder dome and bladder base. Among the changes in AC activities, PGE1 and PGE2 significantly increased the AC activities of the renal pelvis and ureter. PGE1 significantly increased the AC activities of the bladder dome. It was reported that Ca2+ influx plays an important role in the PGs' action on the urinary tract. Our data suggest that the PGs act on the urinary tract via cAMP as well as Ca2+ influx.
Second Department of Biochemistry, Akita University School of Medicine.
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Kondo
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Morita
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Hirano
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D015237:Dinoprost / Q000494:pharmacology*; D015232:Dinoprostone / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D014551:Urinary Tract / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism
D015237:Dinoprost; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D000527:Alprostadil; D015232:Dinoprostone; D002118:Calcium