Inhibition of glycoprotein traffic through the secretory pathway by ceramide.
Incubation of vesicular stomatitis virus-infected cells with short-chain, cell-permeable ceramide (Cer) analogs decreased the rate of viral glycoprotein transport through the Golgi complex and reduced the number of infectious virions released from cells in a concentration-dependent manner. These effects appeared to be caused directly by Cer, rather than by one of its metabolites. Cer treatment also disrupted the Golgi apparatus within 1 h, although cells treated for up to 24 h with Cer remained viable. Our results suggest that endogenous Cer may modulate secretory protein traffic and that exogenously added Cer analogs may be useful as antiviral agents.
Rosenwald
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Carnegie Institution of Washington, Baltimore, Maryland 21210.
Pagano
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D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D016466:CHO Cells; D002518:Ceramides / Q000494:pharmacology*; D006224:Cricetinae; D006023:Glycoproteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion
D002518:Ceramides; D006023:Glycoproteins; D014764:Viral Proteins