Molecular basis of the little mouse phenotype and implications for cell type-specific growth.
The molecular basis for the little (lit) mouse phenotype, characterized by a hypoplastic anterior pituitary gland, is the mutation of a single nucleotide that alters Asp 60 to Gly in the growth hormone releasing factor receptor. Detailed analysis of the lit mouse anterior pituitary reveals spatially distinct proliferative zones of growth hormone-producing stem cells and mature somatotrophs, each regulated by a different trophic factor. This sequential growth factor requirement for a specific cell type may exemplify a common strategy for regulating cellular proliferation in other mammalian organs.
Howard Hughes Medical Institute, School of Medicine, University of California, San Diego, La Jolla 92093-0648.
S C
SC
Lin
C R
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Lin
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I
Gukovsky
A J
AJ
Lusis
P E
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Sawchenko
M G
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Rosenfeld
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism; D004393:Dwarfism, Pituitary / Q000235:genetics*; D005260:Female; D013007:Growth Hormone-Releasing Hormone / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides; D010641:Phenotype; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000473:pathology; D011401:Promoter Regions, Genetic; D018013:Receptors, Neuropeptide; D017981:Receptors, Neurotransmitter / Q000235:genetics* / Q000502:physiology; D018017:Receptors, Pituitary Hormone-Regulating Hormone; D015398:Signal Transduction / Q000235:genetics; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D013007:Growth Hormone-Releasing Hormone; D009838:Oligodeoxyribonucleotides; D018013:Receptors, Neuropeptide; D017981:Receptors, Neurotransmitter; D018017:Receptors, Pituitary Hormone-Regulating Hormone; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; C494185:Pou1f1 protein, mouse; C039542:somatocrinin receptor