Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein.
Large crystals of the Escherichia coli F plasmid CcdB killer protein were grown from solutions containing 32% ammonium sulphate. The crystals belong to space group P4(2)2(1)2 with a = b = 104.52 A and c = 88.45 A or P2(1)2(1)2(1) with a = 77.62 A, b = 93.28 A and c = 141.44 A. Both crystal forms diffract to 2.6 A resolution. Structure determination by multiple isomorphous replacement is under way.
Steyaert
J
J
Instituut Moleculaire Biologie, Vrije Universiteit Brussel, Belgium.
Van Melderen
L
L
Bernard
P
P
Thi
M H
MH
Loris
R
R
Wyns
L
L
Couturier
M
M
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000737:chemistry* / Q000302:isolation & purification; D002942:Circular Dichroism; D003460:Crystallization; D004926:Escherichia coli / Q000737:chemistry*; D005144:F Factor; D059004:Topoisomerase I Inhibitors; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C068356:CcdB protein, Plasmid F; D059004:Topoisomerase I Inhibitors