Cytochrome c550 from Pseudomonas aeruginosa.
In cells of Pseudomonas aeruginosa A.T.C.C. 17933 grown on ethanol the synthesis of a soluble c-type cytochrome, together with quinoprotein ethanol dehydrogenase, is induced. The cytochrome, with an alpha-absorption band at 550 nm, was purified to homogeneity. The molecular mass of the monomeric protein is 15 kDa, the pI is 4.8, and it contains one haem prosthetic group. The midpoint potential of the autoxidizable, but not autoreducible, cytochrome is 280 mV. Cytochrome c550 mediates electron transfer between quinoprotein ethanol dehydrogenase and ferricyanide. In a system composed of membrane particles with NN'NN'-tetramethyl-p-phenylenediamine oxidase activity and quinoprotein ethanol dehydrogenase, oxygen consumption is only observed in the presence of cytochrome c550. This indicates the participation of the cytochrome in the electron-transport chain linked to quinoprotein ethanol dehydrogenase in P. aeruginosa. The electron transport from ethanol dehydrogenase to oxygen is inhibited by myxothiazol and antimycin, indicating that a cytochrome bc1-like complex is involved.
Fachgebiet Technische Biochemie, Institut für Biotechnologie der Technischen Universität Berlin, Federal Republic of Germany.
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Görisch
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D003574:Cytochrome c Group / Q000302:isolation & purification* / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects; D004790:Enzyme Induction; D000431:Ethanol / Q000494:pharmacology; D005292:Ferricyanides / Q000737:chemistry; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D013057:Spectrum Analysis
D003574:Cytochrome c Group; D005292:Ferricyanides; C007931:hexacyanoferrate III; D000431:Ethanol; C031732:cytochrome C-550; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)