Singlet oxygen induces collagenase expression in human skin fibroblasts.
Singlet oxygen generated in a dark reaction by thermodissociation of an endoperoxide (NDPO2) elicits an increase in mRNA of interstitial collagenase (MMP-1) in cultured human fibroblasts. The effect is enhanced in deuterium oxide-based medium and is abolished in the presence of non-toxic doses of sodium azide. In contrast, the mRNA level of the tissue inhibitor of metalloproteinases (TIMP-1) remains unaltered under these experimental conditions. These observations support the suggestion that an unbalanced synthesis of collagenase and TIMP reported to occur following UV-A irradiation or during inflammatory conditions may be mediated by singlet oxygen.
Hautklinik, Heinrich-Heine-Universität Düsseldorf, Germany.
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Scharffetter-Kochanek
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Wlaschek
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Briviba
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Sies
D002478:Cells, Cultured; D017364:Collagenases / Q000235:genetics*; D005347:Fibroblasts; D015870:Gene Expression / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D012867:Skin / Q000201:enzymology*; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D019714:Tissue Inhibitor of Metalloproteinases; D017364:Collagenases