Structural analysis and expression of a chromosomal gene encoding an avian Na+/K(+)-ATPase beta 1-subunit.
Chicken chromosomal DNA encoding the Na+/K(+)-ATPase beta 1-subunit was cloned and characterized. Its exon-intron structure is identical to mammalian (human and rat) beta 1-subunit genes. The transcription initiation site, TATA box, and an ATTGG (antisense CCAAT) sequence follow approximately 1 kilobase of GC-rich 5' upstream sequence that contains many consensus sequences for transcription factors whose relative positions are conserved between human and chicken genes. When this beta 1-subunit gene was stably incorporated into mouse L cells and C2C12 cells, the avian beta 1-subunit was expressed under the control of the its own promoter.
Department of Medical Biochemistry, Ohio State University, Columbus.
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K
Takeyasu
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M
Hamrick
A M
AM
Barnstein
D M
DM
Fambrough
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002875:Chromosomes; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D006801:Humans; D007438:Introns; D007739:L Cells; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D014162:Transfection
D046911:Macromolecular Substances; D004247:DNA; D000254:Sodium-Potassium-Exchanging ATPase