The herpes simplex virus type 1 reactivation function lies outside the latency-associated transcript open reading frame ORF-2.
The latency-associated transcription unit has been shown to be important for in vivo reactivation of herpes simplex virus from the latent state. A recombinant virus was constructed to alter the largest open reading frame in this region. This virus had a wild-type reactivation phenotype, suggesting that herpes simplex virus does not require a protein function from this reading frame for efficient reactivation from latency.
Farrell
M J
MJ
Molecular Biology Institute, School of Medicine, University of California, Los Angeles 90024.
Hill
J M
JM
Margolis
T P
TP
Stevens
J G
JG
Wagner
E K
EK
Feldman
L T
LT
D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic; D014775:Virus Activation / Q000235:genetics*
D004274:DNA, Recombinant; D004279:DNA, Viral