Small introns in a hepatic cDNA encoding a new glucagon-like peptide 1-type receptor.
A cDNA fragment was PCR amplified at low-stringency from rat DNA, using two degenerated primers corresponding to consensus sequences in the second and third transmembrane segments of members of the secretin receptor family. A synthetic oligodeoxynucleotide based on the 3 end of this fragment, when used in Northern-blot analysis, hybridized to a broad 1.5-3.0 kb band in rat liver. It was then used as a probe for screening a cDNA library generated from size-fractionated rat liver poly(A)+ RNA. Overlapping alignment of sequences from 3 clones identified a 1455 residue open reading frame encoding a 485 amino acid peptide showing 7 putative transmembrane segments and 42% identity with the glucagon-like peptide 1 (GLP-1) receptor. In addition, four small (85-300 bp) introns were variously represented in the isolated clones.
Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.
M
M
Svoboda
E
E
Ciccarelli
M
M
Tastenoy
A
A
Cauvin
M
M
Stiévenart
J
J
Christophe
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D000067757:Glucagon-Like Peptide-1 Receptor; D007438:Introns; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics; D018027:Receptors, Glucagon; D017386:Sequence Homology, Amino Acid
C000593835:Glp1r protein, rat; D000067757:Glucagon-Like Peptide-1 Receptor; D011956:Receptors, Cell Surface; D018027:Receptors, Glucagon; D004247:DNA