The 1,629-nucleotide open reading frame located downstream of the Autographa californica nuclear polyhedrosis virus polyhedrin gene encodes a nucleocapsid-associated phosphoprotein.
A 78-kDa protein was produced in bacteria from a clone of the 1,629-nucleotide open reading frame located immediately downstream from the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. The identity of this protein was confirmed by its reactivity with peptide antiserum and amino terminal peptide sequencing after purification from transformed bacteria. The polypeptide was used to produce polyclonal antisera in rabbits. Immunoblot analysis of insect cells infected with the baculovirus indicated that two related proteins with molecular masses of 78 and 83 kDa were synthesized late in infection. Biochemical fractionation studies indicated that both of these proteins were present in purified nucleocapsids from budded and occluded virus preparations. Immunoprecipitation of 32P-labeled proteins and treatment of purified nucleocapsids with alkaline phosphatase demonstrated that the 83-kDa protein was a phosphorylated derivative of the 78-kDa protein. Furthermore, immunoelectron microscopy revealed that the proteins were localized to regions of nucleocapsid assembly within the infected cell and appeared to be associated with the end structures of mature nucleocapsids.
Department of Microbiology and Immunology, McGill University, Montréal, Québec, Canada.
J E
JE
Vialard
C D
CD
Richardson
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009036:Moths; D000077183:Occlusion Body Matrix Proteins; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics*; D015678:Viral Structural Proteins
D000077183:Occlusion Body Matrix Proteins; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus