Excitatory effect of propentofylline on neurotransmission in guinea pig hippocampal slice.
The postsynaptic field potential (population spike; PS) was recorded from the granule cell layer of guinea pig hippocampal slices after electrical stimulation of the perforant path. Propentofylline, a xanthine derivative, enhanced the amplitude of PS to 120-150% of the original level at a concentration range of 100 nM to 1 mM. Theophylline at concentrations over 10 microM increased the PS amplitude to 200% of the original amplitude. Simultaneous application of 1-(5-isoquin-olinesulfonyl)-2-methylpiperazine dihydrochloride (H-7; 100 microM), a protein kinase C (PKC) inhibitor, significantly blocked the excitatory effect of propentofylline, but it failed to counteract the excitatory effect of theophylline. These results indicate that the excitatory effect of propentofylline is mediated by a metabolic process involving PKC and that the protective effect of propentofylline on neuronal damage caused by hypoxia is not due to the inhibition of synaptic transmission.
Department of Physiology, School of Medicine, Kobe University, Japan.
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Kimura
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Yamada
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Okada
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects*; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D010477:Perfusion; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D013569:Synapses / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects*; D013806:Theophylline / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology*
D000897:Anti-Ulcer Agents; D007546:Isoquinolines; D010879:Piperazines; D014970:Xanthines; C032114:propentofylline; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D013806:Theophylline; D011493:Protein Kinase C