Distinct functions of SR proteins in alternative pre-mRNA splicing.
Alternative splicing of precursor messenger RNAs (pre-mRNAs) is a common mechanism of regulating gene expression. SR proteins are a family of pre-mRNA splicing factors that are structurally related and evolutionarily conserved. Any member of the SR family can complement a splicing-deficient extract that lacks the entire family of SR proteins. Here it is demonstrated that particular SR proteins have distinct functions in alternative pre-mRNA splicing in vitro. In addition, SR proteins are differentially expressed in a variety of tissues. These results suggest a fundamental role for SR proteins in the regulation of alternative splicing.
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
A M
AM
Zahler
K M
KM
Neugebauer
W S
WS
Lane
M B
MB
Roth
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D002457:Cell Extracts; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing; D012367:RNA, Viral / Q000235:genetics; D016601:RNA-Binding Proteins / Q000502:physiology*; D013539:Simian virus 40 / Q000235:genetics
D002457:Cell Extracts; D012322:RNA Precursors; D012367:RNA, Viral; D016601:RNA-Binding Proteins