Comparison of L1 consensus primers with E6 type specific primers for detection of human papillomaviruses in paraffin sections of cervical neoplasia.
In our comparative study of L1 consensus primers with E6 type-specific primers for detection of human papillomavirus (HPVs) by polymerase chain reaction (PCR) in 35 cases of cervical neoplasia, the detection rate by E6 primers (54%; 19/35) was significantly higher than that by L1 primers (25%; 9/35) (p < 0.01). And all specimens HPV-positive with L1 primers were also positive by E6 primers. HPV DNA could be amplified in 36% (9 of 25) of tissue by L1 consensus primers from which beta-globin gene was amplified as compared with 64% (16 of 25) of tissue by E6 type-specific primers. With the L1 consensus primers, 8 cases were positive for HPV-16 and 1 case was positive for HPV-33. These results show that the L1 consensus primers have inferior sensitivity to the E6 type-specific primers for the detection of HPV by PCR. But the L1 consensus primers have great value in making simultaneous detection of various HPV types in a single tube reaction, thus they permit reduction of time and the economic burden of the experiment.
Department of Obstetrics and Gynecology, Catholic University Medical College, Seoul, Korea.
J S
JS
Park
S E
SE
Namkoong
S K
SK
Han
D J
DJ
Nha
H Y
HY
Lee
S J
SJ
Kim
D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000382:microbiology*
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides