Activation of four enzymes by two series of calmodulin mutants with point mutations in individual Ca2+ binding sites.
Activation of four target enzymes by two series of calmodulin Ca2+ binding site mutants has been examined. In each mutant, the conserved bidentate glutamate of one of the Ca2+ binding sites is mutated to glutamine or lysine. The enzymes studied were smooth and skeletal muscle myosin light chain kinases, adenylylcyclase, and plasma membrane Ca(2+)-ATPase. For the first three enzymes, the activation patterns with the two mutant series were very similar: mutation of site 4 was most deleterious, then site 2, site 3, and site 1. This ranking was observed previously in Ca2+ binding and Ca(2+)-induced conformational studies of these mutants. Thus the response of these enzymes is probably determined by the extent to which each mutant's competence to interact with target binding regions has been compromised. In contrast, for Ca(2+)-ATPase, mutants of sites 3 and 4 were much poorer activators than those of sites 1 and 2. Events beyond calmodulin binding and related to enzyme activation probably dictate this unusual activation pattern and also the anomalously poor activation of skeletal muscle myosin light chain kinase by site 1 mutant B1Q. Site 1 mutant B1K showed wild type activation of all four enzymes suggesting that in site 1, the lysine substitution can evoke the conformational changes associated with Ca2+ binding.
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235.
Z H
ZH
Gao
J
J
Krebs
M F
MF
VanBerkum
W J
WJ
Tang
J F
JF
Maune
A R
AR
Means
J T
JT
Stull
K
K
Beckingham
D000262:Adenylyl Cyclases / Q000096:biosynthesis / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D001665:Binding Sites; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000097:blood* / Q000302:isolation & purification; D002147:Calmodulin / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D002417:Cattle; D002645:Chickens; D004789:Enzyme Activation; D004910:Erythrocyte Membrane / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009036:Moths; D009132:Muscles / Q000201:enzymology; D016297:Mutagenesis, Site-Directed; D009219:Myosin-Light-Chain Kinase / Q000302:isolation & purification / Q000378:metabolism*; D017354:Point Mutation; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014162:Transfection
D002147:Calmodulin; D011994:Recombinant Proteins; D009219:Myosin-Light-Chain Kinase; D000262:Adenylyl Cyclases; D000252:Calcium-Transporting ATPases; D002118:Calcium