Research note: the S(al)-c mutation in the chicken is not linked to Rous-associated virus-2 related DNA sequences.
A male chicken heterozygous for a gene for sex-linked imperfect albinism (s(al)-c) was used to produce offspring in five dam families. The DNA from 23 offspring (22 females and 1 male) was cut with the restriction enzyme Sac1 and hybridized with a Rous-Associated Virus-2 probe to test for linkage between the gene and endogenous viral (ev) genes. In a second experiment, 15 albino and 15 nonalbino females resulting from 4 males mated to 11 females were studied with the enzymes Sac1 and BamHI. In the first experiment, 10 different ev genes were seen. These, plus seven additional bands were seen in the second experiment. The gene s(al)-c segregated independently of all ev genes, suggesting that it could be used for autosexing of chickens or in layer flocks without causing inherent problems previously associated with K, the gene for slow feathering, caused by essentially complete concordance between K and ev21.
Laboratoire de Génétique Factorielle, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
F G
FG
Silversides
M
M
Tixier-Boichard
L
L
Durand
P
P
Mérat
D000417:Albinism / Q000235:genetics / Q000662:veterinary*; D000818:Animals; D001345:Autoradiography; D001354:Avian Leukosis Virus / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000382:microbiology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; C024408:endodeoxyribonuclease SacI; D015252:Deoxyribonucleases, Type II Site-Specific