[A comparative study of the antioxidant properties of polyhydroxamic acids, desferal and other iron chelating agents].
The effects of different iron chelators on both OH.-generation in Fenton reaction and lipid peroxidation products accumulation in brain homogenate were studied. Intensity of luminol-dependent chemiluminescence was the measure of OH.-radical generation. The rate of lipid peroxidation was estimated as malondialdehyde accumulation. It was shown, that inhibition of chemiluminescence by the tested substances increased in next series: ADP, polyhydroxamic acid (PHA), EDTA, desferal, o-phenanthroline, ascorbate. Inhibition of lipid peroxidation by these substances increased in other order: ADP, Ascorbate, EDTA, PHA, desferal, o-phenanthroline. The results obtained allow to conclude, that antioxidant activity of ascorbate is due to the trapping of OH. radicals; in contrast, inhibition of the free radical processes by other tested substances is due to binding of iron ions. Antioxidant effect of PHA is almost as strong as effects of desferal, o-phenanthroline and EDTA. The lowest antioxidant activity was found in the presence of ADP.
A V
AV
Kozlov
F Iu
FIu
Meshchanov
I G
IG
Nikolaeva
G N
GN
Kol'tsova
Iu A
IuA
Vladimirov
D000975:Antioxidants / Q000494:pharmacology*; D003676:Deferoxamine / Q000494:pharmacology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D006878:Hydroxides; D017665:Hydroxyl Radical; D007502:Iron Chelating Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008163:Luminescent Measurements
D000975:Antioxidants; D006877:Hydroxamic Acids; D006878:Hydroxides; D007502:Iron Chelating Agents; D017665:Hydroxyl Radical; D003676:Deferoxamine