Formaldehyde-fixed semen is suitable and safer for leukocyte detection and DNA amplification.
This pilot study indicates the use of formalin-fixation may offer a significant advance in the cytologic and pathological evaluation of semen. We are reporting this novel method to stimulate further assessment of this safer approach to semen analysis. The immunohistochemistry and PCR methods described here may also prove useful in improving the safety of studies of pathogen-infected cells in other laboratory settings.
Faulkner Hospital, Boston, Massachusetts.
A A
AA
Kiessling
H Z
HZ
Yin
A
A
Purohit
M
M
Kowal
B
B
Wolf
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D005404:Fixatives / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology*; D006678:HIV / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000166:cytology*; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012661:Semen / Q000166:cytology* / Q000502:physiology*
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D005404:Fixatives; D015335:Molecular Probes; D005557:Formaldehyde