Na,K-ATPase in several tissues of the rat: tissue-specific expression of subunit mRNAs and enzyme activity.
The relative contents of Na,K-ATPase subunit mRNAs in rat renal cortex, ventricular myocardium, skeletal muscle (hind limb), liver and brain (cerebrum) were measured. Expressed per unit DNA, mRNA alpha 1 content was approximately 2-fold greater in the kidney and brain as compared to either heart, skeletal muscle or liver. The hierarchy of mRNA alpha 2 expression was brain > skeletal muscle > heart, whereas mRNA alpha 3 was restricted to brain. Beta 1 subunit mRNA content in both kidney and brain exceeded the abundance of liver mRNA beta 1 by approximately 7-fold. In all tissues examined, the combined abundances of the alpha subunit mRNAs exceeded the content of mRNA beta 1. The hierarchy of Na,K-ATPase activity expressed per unit DNA was brain > kidney > skeletal muscle = heart > liver. The sum of mRNA alpha as well as mRNA beta 1 content, expressed per g of tissue, was highest in brain and kidney. A statistically significant correlation between mRNA beta 1 content and Na,K-ATPase activity was evident.
Department of Biochemistry, State University of New York Health Science Center, Brooklyn 11203.
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Ismail-Beigi
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry; D004247:DNA / Q000032:analysis / Q000235:genetics; D004789:Enzyme Activation; D007672:Kidney Cortex / Q000737:chemistry* / Q000201:enzymology*; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000737:chemistry* / Q000201:enzymology*; D009206:Myocardium / Q000737:chemistry* / Q000201:enzymology*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology
D012333:RNA, Messenger; D004247:DNA; D000254:Sodium-Potassium-Exchanging ATPase