Induction of phosphatidylinositol-linked Fc gamma receptor III expression on an eosinophilic cell line, EoL-1, by dibutyryl cyclic AMP and interferon-gamma.
The expression of Fc gamma receptor III (Fc gamma RIII) on a human eosinophilic leukemia cell line, EoL-1, was examined and compared with its expression on normal blood eosinophils. Surface Fc gamma RIII expression on EoL-1 cells could be induced in vitro with a combination of dibutyryl cAMP (dbcAMP) and gamma-interferon (IFN-gamma), but not with IFN-gamma or dbcAMP alone. Pretreatment of EoL-1 cells with dbcAMP induced EoL-1 cells to express Fc gamma RIII when stimulated with IFN-gamma, but EoL-1 cells pretreated with IFN-gamma and then stimulated with dbcAMP failed to express Fc gamma RIII. Cyclic AMP was shown to play a role in the effect of dbcAMP. Both the treatment with phosphatidyl-inositol-specific phospholipase C (PI-PLC) and the restriction enzyme digestion of Fc gamma RIII cDNA showed that the Fc gamma RIII on EoL-1 cells was a phosphatidylinositol-linked form. On the other hand, freshly isolated blood eosinophils constitutively expressed few, if any, Fc gamma RIII, and IFN-gamma induced Fc gamma RIII expression on them in vitro. Dibutyryl cAMP did not induce Fc gamma RIII expression and even suppressed the IFN-gamma-induced Fc gamma RIII expression on normal eosinophils. The EoL-1 cell line appears to be a useful in vitro model for the expression and function of the phosphatidylinositol-linked form of Fc gamma RIII on eosinophils.
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Y
Y
Ohshima
M
M
Nambu
E Y
EY
Jung
H
H
Akutagawa
T
T
Heike
K
K
Katamura
M
M
Mayumi
H
H
Mikawa
D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D004357:Drug Synergism; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D017452:Receptors, IgG / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins; D013268:Stimulation, Chemical
D010716:Phosphatidylinositols; D017452:Receptors, IgG; D011994:Recombinant Proteins; D003995:Dibutyryl Cyclic GMP; D003994:Bucladesine; D007371:Interferon-gamma