High-level production of an active single-chain Fv fragment in the culture supernatant of Escherichia coli.
Sizmann
D
D
VTT Biotechnical Laboratory, Espoo, Finland.
Takkinen
K
K
Laukkanen
M L
ML
Saloheimo
M
M
Candussio
A
A
Veijola-Bailey
P
P
Teeri
T T
TT
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010081:Oxazolone / Q000031:analogs & derivatives / Q000276:immunology; D011133:Polysaccharide-Lyases / Q000235:genetics; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D013205:Staphylococcal Protein A / Q000235:genetics
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007135:Immunoglobulin Variable Region; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; C034151:heat stable toxin (E coli); C018361:2-phenyloxazolone; D010081:Oxazolone; D011133:Polysaccharide-Lyases; C074124:pectin lyase B