Effect of influenza A/equine/H3N8 virus isolate variation on the measurement of equine antibody responses.
This study has tested the effect of using homologous or heterologous equine influenza A virus isolates to evaluate serum antibody levels to influenza A virus in vaccinated and naturally-infected horses. In addition, the potential effect of antigenic selection of virus variants in egg versus tissue culture propagation systems was studied. Serum antibody levels in samples from horses recently infected with a local influenza A virus isolate (A/equine 2/Saskatoon/1/90) or recently vaccinated with a prototype isolate (A/equine 2/Miami/1/63) were assessed by hemagglutination inhibition and by single radial hemolysis using cell or egg-propagated A/equine 2/Saskatoon/1/90, A/equine 2/Miami/1/63 or A/equine 2/Fontainebleau/1/79. There were no significant differences in hemagglutination inhibition or single radial hemolysis antibody levels obtained with homologous or heterologous isolates or between viruses propagated in either eggs or cell culture. However there was a trend to higher titers in the hemagglutination inhibition assay when cell-propagated virus was used. These results suggest that antigenic variation in equine influenza A virus isolates and host-cell selection of antigenic variants during virus propagation may not be of sufficient magnitude to influence serological evaluation of antibody responses by hemagglutination inhibition or single radial hemolysis.
Equine Respiratory Disease Research Group, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
J R
JR
Bogdan
P S
PS
Morley
H G
HG
Townsend
D M
DM
Haines
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D006462:Hemolytic Plaque Technique; D006734:Horse Diseases / Q000276:immunology*; D006736:Horses; D053123:Influenza A Virus, H3N8 Subtype; D009980:Influenza A virus / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines