Expression of the cholecystokinin gene in organotypic slice cultures of immature rat somatosensory cortex.
The preprocholecystokinin gene is expressed in a subpopulation of cortical interneurons containing gamma-aminobutyric acid. Slices of neonatal rat cortex were cultivated for 12 +/- 2 days and examined for the presence and distribution of these neurons by in situ hybridization and immunocytochemistry. Like in situ, two layers of preprocholecystokinin-mRNA-expressing cells were present. Immunopositive fibers formed a dense network and established symmetric contacts on dendritic shafts and spines. It is concluded that cholecystokinin-expressing interneurons survive in cultured slices of rat cerebral cortex. These organotypic cultures may be useful to study the cellular interactions which regulate neuronal cholecystokinin expression.
Institute of Pharmacology, University of Freiburg, FRG.
C
C
Olenik
B
B
Heimrich
D K
DK
Meyer
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002470:Cell Survival; D002766:Cholecystokinin / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression; D007395:Interneurons / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013003:Somatosensory Cortex / Q000378:metabolism*
D011498:Protein Precursors; D012333:RNA, Messenger; C042082:preprocholecystokinin; D002766:Cholecystokinin