Micro liquid chromatography-mass spectrometry with direct liquid introduction used for separation and quantitation of all-trans- and 13-cis-retinoic acids and their 4-oxo metabolites in human plasma.
The separation and quantitation of the pentafluorobenzyl derivatives of all-trans- and 13-cis-retinoic acids and their 4-oxo metabolites in human plasma on micro high-performance liquid chromatographic columns (0.32 mm I.D.) is described. The column outlet was directly coupled to the source of a quadrupole mass spectrometer via a simple SFC-frit interface. Negative ion chemical ionization conditions were obtained by coaxial introduction of ammonia as a reagent gas. A signal-to-noise ratio well above 3 was obtained for 1 pg of each analyte injected. The limit of quantitation calculated from spiked biological plasma extracts was 0.3 ng/ml.
Ranalder
U B
UB
Department of New Technologies, Hoffmann-La Roche AG, Basel, Switzerland.
Lausecker
B B
BB
Huselton
C
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015474:Isotretinoin / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D014212:Tretinoin / Q000097:blood* / Q000652:urine
D014212:Tretinoin; D015474:Isotretinoin