The behavior of free purine nucleotides in lymphocytes infected with HIV-1 virus.
The purine nucleotide content was examined in various cells before and after HIV-1 virus infection: healthy peripheral blood lymphocytes (PBL) and cultured PBL after infection; the PBL of asymptomatic, ARC and AIDS patients. In all cases, changes in purine nucleotide concentrations were observed. The pattern of purine nucleosides and nucleobases was also evaluated by HPLC in PBL of controls and patients. The analysis was integrated by following the incorporation of a labelled precursor ([14C]formate) into purine nucleotides, which was investigated as an indication of the rate of purine metabolism in these cells. Many interesting variations in the catabolic and of anabolic pathways were observed, demonstrating that the viral penetration affects purine nucleotide metabolism. These results suggest interesting perspectives in AIDS research.
Istituto di Biochimica e di Enzimologia, Università di Siena, Italy.
A
A
Tabucchi
F
F
Carlucci
M C
MC
Re
G
G
Furlini
E
E
Consolmagno
R
R
Leoncini
M
M
Pizzichini
E
E
Marinello
M
M
Rubino
R
R
Pagani
D000386:AIDS-Related Complex / Q000378:metabolism*; D000163:Acquired Immunodeficiency Syndrome / Q000378:metabolism*; D000328:Adult; D002470:Cell Survival; D005260:Female; D006679:HIV Seropositivity / Q000378:metabolism*; D015497:HIV-1; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011685:Purine Nucleotides / Q000378:metabolism*; D011742:Pyrimidine Nucleotides / Q000378:metabolism
D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides