Calcitonin gene-related peptide (CGRP)1 receptor mediates vasodilation in the rat isolated and perfused kidney.
The vasodilator activity of rat(r) alpha and beta calcitonin gene-related peptide (CGRP) as compared to the structurally related peptide, rAmylin, [Cys(ACM)2,7]human (h)CGRP and salmon calcitonin, was investigated in the rat isolated and perfused kidney which vascular tone was increased by 3 microM noradrenaline. The order of potency in producing vasodilation was alpha CGRP > beta CGRP >> rAmylin. [Cys(ACM)2,7]hCGRP and salmon calcitonin were ineffective. The activity of alpha and beta forms of rCGRP and rAmylin was antagonized by hCGRP (8-37), a CGRP1 receptor antagonist, at a concentration which did not affect acetylcholine-induced vasodilation. These results indicate the involvement of CGRP1 receptor in the CGRP-induced vasodilation in the rat kidney.
Pharmacology Department, Malesci Pharm., Firenze, Italy.
A
A
Castellucci
C A
CA
Maggi
S
S
Evangelista
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects / Q000648:ultrastructure; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*
D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D015740:Calcitonin Gene-Related Peptide; D009638:Norepinephrine