Evaluation of conventional media for detection of colonization factor antigens of enterotoxigenic Escherichia coli.
Strains of enterotoxigenic Escherichia coli producing either colonization factor antigen (CFA) I or II were tested for expression of CFA when grown on 16 different agar media by using agglutination and coagglutination with monoclonal antibodies, mannose-resistant hemagglutination, and a salt aggregation assay. CFA was detected from the CFA-positive strains when CFA agar was used, and it was also detected when other commercially available media were used, notably nutrient agar. CFA was not detected when other commercial media such as MacConkey agar were used. The use of nutrient agar with monoclonal antibody-based coagglutination reagents offers a potentially simple and rapid method for detecting E. coli which express CFA I or II.
National Institute of Cholera and Enteric Diseases, Calcutta, India.
A R
AR
Ghosh
D
D
Sen
D A
DA
Sack
A T
AT
Hoque
D000371:Agglutination; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis* / Q000096:biosynthesis; D003470:Culture Media; D004926:Escherichia coli / Q000472:pathogenicity*; D036781:Fimbriae Proteins
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; C022924:colonization factor antigens; D036781:Fimbriae Proteins