Reversible changes in the isoenzyme electrophoretic mobility pattern and infectivity in clones of Trypanosoma cruzi.
The stability of zymodemes in clonal cultures of Trypanosoma cruzi derived from strain Y was followed. Reversible changes in the isoenzyme electrophoretic mobility pattern from type A to types B and C were observed after subculturing of cloned cultures in medium of different composition or after passage in newborn mice. Type A zymodeme was observed in clones grown in blood-containing media, while types B and C were found in clones and subclones grown in media progressively less rich in nutrients (10 and 5% fetal calf serum, respectively) and containing no blood. The change in zymodeme from type A to type B or C was associated with loss of infectivity, which could be recovered by passages in newborn mice. Parasites infective for mice always showed zymodeme A. Simultaneously with zymodeme change from type A to types B and C there is a decrease in the specific activity of G6PD and 6PGD.
Alves
A M
AM
Laboratório de Fisiologia Celular, Universidade Federal do Rio de Janeiro, Brazil.
Tanuri
A
A
de Almeida
D F
DF
von Krüger
W M
WM
D000818:Animals; D003001:Cloning, Molecular; D004586:Electrophoresis; D007527:Isoenzymes / Q000032:analysis*; D051379:Mice; D014349:Trypanosoma cruzi / Q000145:classification / Q000201:enzymology* / Q000472:pathogenicity
D007527:Isoenzymes