Atrial natriuretic peptide inhibits endothelin-1-induced activation of mitogen-activated protein kinase in cultured rat mesangial cells.
In cultured rat glomerular mesangial cells, endothelin-1 (ET-1) activated both pp 44 and pp 42 mitogen-activated protein (MAP) kinases. Atrial natriuretic peptide (ANP) inhibited ET-1-induced activation of both pp 44 and pp 42 MAP kinases. ANP also inhibited ET-1-induced translocation of protein kinase C (PKC) and TPA-induced activation of MAP kinase. These results indicate that ANP modulates the functions of mesangial cells, including proliferation and contraction through the inhibition of ET-1-induced activation of MAP kinase in various steps proximal to MAP kinase.
Third Department of Medicine, Shiga University of Medical Science, Japan.
T
T
Sugimoto
R
R
Kikkawa
M
M
Haneda
Y
Y
Shigeta
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002478:Cells, Cultured; D016232:Endothelins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004789:Enzyme Activation; D005920:Glomerular Mesangium / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016232:Endothelins; D010750:Phosphoproteins; D015240:Phorbol 12,13-Dibutyrate; D009320:Atrial Natriuretic Factor; D011494:Protein Kinases; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases