Analysis of the tumorigenic potential of common marmoset lymphoblastoid cells expressing a constitutively activated c-myc gene.
The respective roles of Epstein-Barr virus (EBV) and c-myc in the pathogenesis of endemic Burkitt's lymphoma (BL) are unclear. In order to help resolve the question whether constitutive expression of the c-myc gene in an EBV-immortalised B cell is sufficient to induce a tumorigenic phenotype, B cells from a common marmoset (Callithrix jacchus) were immortalised with EBV, transfected with a constitutively activated c-myc gene and inoculated into the host animals. Despite the cell line transfected with c-myc displaying enhanced growth characteristics, in vitro and in vivo experiments demonstrated that this was not sufficient to induce a tumorigenic phenotype. This supports our previous findings with EBV-immortalised human B cells transfected with an activated c-myc gene (Hotchin et al., 1990).
Hotchin
N A
NA
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, UK.
Wedderburn
N
N
Roberts
I
I
Thomas
J A
JA
Bungey
J A
JA
Naylor
B
B
Crawford
D H
DH
D000818:Animals; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000473:pathology*; D002144:Callithrix; D002472:Cell Transformation, Viral; D005260:Female; D015870:Gene Expression; D016259:Genes, myc; D004854:Herpesvirus 4, Human; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000473:pathology
D009838:Oligodeoxyribonucleotides; D016271:Proto-Oncogene Proteins c-myc; D012334:RNA, Neoplasm