Vitamin E recycling in human erythrocyte membranes.
Vitamin E, the major lipid chain-breaking antioxidant in erythrocyte membranes, is present in low concentration, suggesting that mechanisms should exist to protect against its loss. Enzymatic pathways for the recycling of vitamin E from its tocopheroxyl radical have been observed previously in inner membranes of mitochondria and microsomes. These pathways use electron transport enzymes and their substrates to regenerate vitamin E. Erythrocyte membranes also contain significant NADH-cytochrome c reductase activity, as well as cytochrome b5, the function of which is not yet known. Using an enzymatic oxidation system composed of lipoxygenase and arachidonic acid, free radicals were produced in human erythrocyte membranes, and their reaction with chromanols was followed by ESR and high performance liquid chromatography (HPLC). Since the endogenous vitamin E content of the membranes is very low, we used a vitamin E homologue lacking the hydrocarbon chain (2,2,5,7,8-pentamethyl-6-hydroxychromane) as a probe molecule for ESR measurements. However, parallel HPLC determinations of lipid hydroperoxides and of endogenous vitamin E confirmed the results obtained by ESR. It was found that protection against the loss of vitamin E can be provided either by NADH-cytochrome b5-dependent enzymatic recycling or by a nonenzymatic pathway involving ascorbate and dihydrolipoic acid.
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
A
A
Constantinescu
D
D
Han
L
L
Packer
D016718:Arachidonic Acid / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D015786:Cytochromes b5 / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005609:Free Radicals / Q000097:blood; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation; D008084:Lipoxygenase / Q000097:blood; D008954:Models, Biological; D009243:NAD / Q000097:blood; D009245:NADH Dehydrogenase / Q000097:blood; D010084:Oxidation-Reduction; D014810:Vitamin E / Q000097:blood*
D005609:Free Radicals; D009243:NAD; D014810:Vitamin E; D016718:Arachidonic Acid; D015786:Cytochromes b5; D008084:Lipoxygenase; D009245:NADH Dehydrogenase