Conditional cell ablation in Drosophila.
Targeting of cell ablation agents under the control of tissue-specific promoters promises to be an important tool for studies of development and function in higher organisms. Temperature-sensitive cell ablation agents, recently developed for Drosophila, extend control to temporal as well as spatial aspects of toxin expression. Here we discuss achievements to date, together with a novel form of enhancer trap technology with the potential for driving toxin expression in a large range of cell types.
Department of Genetics, University of Glasgow, UK.
J W
JW
Sentry
M M
MM
Yang
K
K
Kaiser
D000818:Animals; D016923:Cell Death / Q000235:genetics*; D004167:Diphtheria Toxin / Q000096:biosynthesis / Q000235:genetics / Q000633:toxicity*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D017344:Genes, Insect; D016153:Genes, Suppressor; D005821:Genetic Techniques; D008562:Membrane Glycoproteins / Q000235:genetics; D009024:Morphogenesis / Q000187:drug effects / Q000235:genetics; D009928:Organ Specificity; D010786:Photoreceptor Cells / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020794:Receptor Protein-Tyrosine Kinases; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000633:toxicity*; D012243:Rhodopsin; D012276:Ricin / Q000096:biosynthesis / Q000235:genetics / Q000633:toxicity*
D004167:Diphtheria Toxin; D029721:Drosophila Proteins; D005136:Eye Proteins; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; C055093:chp protein, Drosophila; C083315:ninaE protein, Drosophila; D012243:Rhodopsin; D012276:Ricin; D020794:Receptor Protein-Tyrosine Kinases; C056737:sev protein, Drosophila