Determination of phloroglucinol in human plasma by gas chromatography-mass spectrometry.
A specific and sensitive method has been developed for the determination of phloroglucinol in plasma; it involves an optimized procedure for blood sampling designed to minimize the in vitro oxidation of the molecule, and gas chromatography-mass spectrometry after silylation of the compound. The method allowed a reliable determination of phloroglucinol in plasma. The precision and accuracy of the assay, reported as coefficients of variation, were below 15%. Using a plasma sample of 0.25 ml, the limit of quantitation was 5 ng/ml with a precision of 17.4%, which is sensitive enough for pharmacokinetic studies. Stability studies under different conditions revealed that ascorbic acid limits the degradation of phloroglucinol in plasma during storage at freezer temperatures.
Lartigue-Mattei
C
C
Laboratoire de Chimie Analytique et de Spectrométrie de Masse, Faculté de Pharmacie, Clermont Ferrand, France.
Lauro-Marty
C
C
Bastide
M
M
Berger
J A
JA
Chabard
J L
JL
Goutay
E
E
Aiache
J M
JM
D004355:Drug Stability; D004356:Drug Storage; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D010696:Phloroglucinol / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results
D010696:Phloroglucinol