The ftf gene encoding the cell-bound fructosyltransferase of Streptococcus salivarius ATCC 25975 is preceded by an insertion sequence and followed by FUR1 and clpP homologues.
Analysis of the region downstream of the ftf gene of Streptococcus salivarius led to the detection of two open reading frames (ORFs). The deduced amino acid sequences of these ORFs were homologous to proteins encoded by genes not previously described and/or sequenced in Gram-positive bacteria. The deduced amino acid sequence of the first of these (orf2) showed strong homology to the product of the FUR1 gene of Saccharomyces cerevisiae, which codes for a uracil phosphoribosyltransferase. The over-expression of the product of this gene appeared to be the source of the detrimental effect observed with phagemids carrying orf2 in Escherichia coli hosts. The deduced amino acid sequence of the second ORF (orf3) was homologous to the ClpP family of proteases. Examination of the upstream region of the ftf gene led to the discovery of a new insertion sequence-like element which has been designated IS1161.
Institute of Dental Research, New South Wales, Australia.
P M
PM
Giffard
C
C
Rathsam
E
E
Kwan
D W
DW
Kwan
K L
KL
Bunny
S P
SP
Koo
N A
NA
Jacques
D049069:ATP-Dependent Proteases; D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D004251:DNA Transposable Elements / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics*; D006602:Hexosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010430:Pentosyltransferases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics*; D013291:Streptococcus / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins
D049069:ATP-Dependent Proteases; D004251:DNA Transposable Elements; D006360:Heat-Shock Proteins; D006602:Hexosyltransferases; D010430:Pentosyltransferases; D012697:Serine Endopeptidases; C021451:inulosucrase; D001426:Bacterial Proteins; C023269:uracil phosphoribosyltransferase