Protein-induced bending as a transcriptional switch.
The question of whether protein-induced DNA bending can act as a switch factor when placed upstream of an array of promoters located in tandem was investigated in vivo. The catabolite activating protein binding site of the fur operon was replaced by the binding site of the RepA repressor protein, which is able to bend DNA immediately after binding. Appropriately phased induced bending could act as a transcriptional switch factor in vivo.
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientifícas, Madrid, Spain.
J
J
Pérez-Martín
M
M
Espinosa
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004265:DNA Helicases; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D011401:Promoter Regions, Genetic; D011506:Proteins; D012097:Repressor Proteins / Q000235:genetics; D015534:Trans-Activators; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D011506:Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; C068477:ferric uptake regulating proteins, bacterial; C045264:replication initiator protein; D012321:DNA-Directed RNA Polymerases; D004265:DNA Helicases