Oxytocin induced cAMP-dependent protein kinase activation and urokinase-type plasminogen activator production in LLC-PK1 renal epithelial cells is mediated by the vasopressin V2-receptor.
Using a variety of peptide analogues of oxytocin (OT) and Arg8-vasopressin (AVP), OT-mediated induction of urokinase-type plasminogen activator (uPA) was examined in LLC-PK1 renal epithelial cells, which possess distinct high-affinity receptors of both the OT- and vasopressin renal (V2-) types. OT or OT-receptor specific agonists induced concentration-dependent cAMP synthesis, activation of the cAMP-dependent protein kinase (cAMP-PK) and uPA production consistent with their respective binding affinities for the V2- and not the OT-receptor. OT-mediated uPA induction could be inhibited in a concentration-dependent fashion by coincubation with a V2/V1-receptor specific antagonist, but not by an OT-receptor specific antagonist. Results implied that stimulation of cAMP- and uPA responses in LLC-PK1 cells by OT was V2-receptor-mediated.
Jans
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DA
Max-Planck-Institut für Biophysik, Frankfurt am Main, Germany.
Pavo
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I
Fahrenholz
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F
D000595:Amino Acid Sequence; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D004790:Enzyme Induction; D004848:Epithelium / Q000502:physiology; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D010121:Oxytocin / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D017483:Receptors, Vasopressin / Q000502:physiology*; D015290:Second Messenger Systems; D013552:Swine; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D009842:Oligopeptides; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D005576:Colforsin; D010121:Oxytocin; D000242:Cyclic AMP; D011494:Protein Kinases; D014568:Urokinase-Type Plasminogen Activator