[Study on the relation of human red cell membrane protein band 4.2 to red cell aging--in cases of obstructive jaundice].
The relation of human red cell membrane protein band 4.2 to red cell aging both in normal controls and in cases of obstructive jaundice was studied. The studied subjects were 9 people aged 20 to 73 years, the serum bilirubin levels of whom ranged from 4.8 to 26.7mg/dl. And normal controls were 9 people aged 20 to 30 years. First, we separated red cells into several fractions depending on red cell aging by the method of Vettore et al. The analyses of polyacrylamide gel electrophoresis (PAGE) of each RBC membrane sample by Fairbanks' system revealed the results as follows. Primarily, it was observed that in comparison with band 4.1, band 4.2 both in normal controls and in cases of obstructive jaundice increased depending on red cell aging. Secondly, it was suggested that the decrease of band 4.2 protein in cases of obstructive jaundice should not be due to the higher level of serum bilirubin but to the lower level of red cell aging. Because the obstructive jaundice could shorten the life span of red cells and make the average aging level of red cells younger.
Department of Laboratory Medicine, Kochi Medical School, Nankoku.
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Hashimoto
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Kageoka
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000032:analysis*; D002779:Cholestasis / Q000097:blood*; D003598:Cytoskeletal Proteins; D004905:Erythrocyte Aging / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008875:Middle Aged
D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C044791:erythrocyte membrane band 4.2 protein