Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay.
A benomyl polyclonal enzyme immunoassay (EIA) commercial kit was used to quantitate methyl 2-benzimidazolecarbamate (MBC), a degradation product of benomyl in wine. Total analysis time, including sample preparation, was 30 min. As many as 8 samples can be analyzed simultaneously with a limit of quantitation of 5 ppb. The assay logarithmic response was linear from 0.4 to 26 ppb MBC. Intra-assay percent coefficients of variation (%CVs) ranged from 2.4 to 13 for standards and from 7.4 to 21 for actual wine samples. Interassay %CVs varied from 2.6 to 15 for the standards and from 6.9 to 23 for the samples. Average recovery from samples spiked at 10-10,000 ppb was 93% for evaporated red and white wines. MBC was determined in 134 different wines by immunoassay and liquid chromatography (LC). Of these samples, 98 were positive for MBC by both methods with a correlation coefficient (r) of 0.986. The other 36 samples had MBC levels that either were not detectable by either procedure or were below the 10 ppb limit of quantitation for LC. Concentrations of MBC in wine ranged from 5 to 1329 ppb, with the majority ranging from 10 to 300 ppb. Also, a mini-study was conducted using the plate EIA format.
Bushway
R J
RJ
University of Maine, Department of Food Science, Orono 04469-5736.
Paradis
L R
LR
Perkins
L B
LB
Fan
T S
TS
Young
B E
BE
Walser
P E
PE
D000818:Animals; D001562:Benzimidazoles / Q000032:analysis*; D002219:Carbamates; D002853:Chromatography, Liquid; D005659:Fungicides, Industrial / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008892:Milk / Q000737:chemistry; D014920:Wine / Q000032:analysis*
D001562:Benzimidazoles; D002219:Carbamates; D005659:Fungicides, Industrial; D007202:Indicators and Reagents; C006698:carbendazim