2,3-butanedione monoxime blockade of Ca2+ currents in adult rat sympathetic neurons does not involve 'chemical phosphatase' activity.
Effects of the 'chemical phosphatase' 2,3-butanedione monoxide (BDM) on voltage-activated Ca2+ currents of adult rat superior cervical ganglion neurons were investigated using the whole-cell patch-clamp technique. BDM produced a rapid (< 10 s), reversible and dose-dependent (IC50 = 18.3 mM) inhibition of Ca2+ currents. The action of BDM was not prevented by 1 mM 8-(4-chlorophenylthio)-cAMP or 50 microM isoproterenol. H-7, a non-specific protein kinase inhibitor at 200 microM, did not prevent the rapid recovery from BDM-induced inhibition. Our results suggest that BDM inhibition of Ca2+ currents in rat sympathetic neurons does not require a 'chemical phosphatase' activity.
Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta 30912-2300.
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Zhu
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Ikeda
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D003931:Diacetyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D004594:Electrophysiology; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000502:physiology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D051381:Rats; D017208:Rats, Wistar; D013873:Thionucleotides / Q000494:pharmacology
D002121:Calcium Channel Blockers; D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D013873:Thionucleotides; C004717:diacetylmonoxime; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000242:Cyclic AMP; D010744:Phosphoric Monoester Hydrolases; D003931:Diacetyl; D007545:Isoproterenol; D002118:Calcium