Effect of herpes simplex virus type-1 UL41 gene on the stability of mRNA from the cellular genes: beta-actin, fibronectin, glucose transporter-1, and docking protein, and on virus intraperitoneal pathogenicity to newborn mice.
Infection with HSV-1 is accompanied by the shut-off of cellular gene expression. The virion-associated function is encoded by the viral gene UL41. An HSV-1 mutant, vhs-1, which has a genomic deletion in the UL41 gene, is incapable of inducing the shut-off of cellular gene expression. The effect of HSV-1 infection on the shut-off of the cellular genes (or mRNA degradation) was studied specifically with the cellular genes for beta-actin, fibronectin, glucose transporter-1, and the docking protein. The level of these specific mRNAs was measured in cells infected with several HSV-1 strains and was compared to that of vhs-1- and mock-infected cells. It was possible to demonstrate a marked reduction in the level of the specific mRNA from these cellular genes in cells infected with several HSV-1 strains but not with the vhs-1 mutant. The pathogenicity of the HSV-1 vhs-1 mutant to newborn mice was studied. It was found that the mutant is less pathogenic to newborn mice than its parental strain HSV-1 KOS.
Department of Molecular Virology, Faculty of Medicine, Hebrew University of Jerusalem, Israel.
Y
Y
Becker
E
E
Tavor
Y
Y
Asher
C
C
Berkowitz
M
M
Moyal
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D005353:Fibronectins / Q000235:genetics; D005814:Genes, Viral; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007274:Injections, Intraperitoneal; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D009154:Mutation; D009842:Oligopeptides / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D018139:Simplexvirus / Q000235:genetics*; D014709:Vero Cells; D014774:Virulence / Q000235:genetics
D000199:Actins; D005353:Fibronectins; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D009842:Oligopeptides; D012333:RNA, Messenger; C035026:SRPRA protein, human; C488951:Srpr protein, mouse; C047981:arginyl-glycyl-aspartic acid