[A new method of isolating acid-stable trypsin inhibitor from human urine].
A novel procedure for isolation of an acid-resistant trypsin inhibitor from human urine (UTI) is described. The method is based on the use of immobilized on an insoluble matrix monoclonal antibodies specific for the given inhibitor. The use of Immotin (which represents an immobilized on Sepharose 4B UTI) as precolumn during affinity chromatography makes it possible to significantly prolong the immunoadsorbent life and to increase the inhibitor yield up to 92% of its original content in the urine. The preparation obtained with the use of an immunoadsorbent contains predominantly the native form of the inhibitor whose molecular mass is about 44 kDa.
Belova
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Ogloblina
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Bekkert
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D000911:Antibodies, Monoclonal; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D014361:Trypsin Inhibitors / Q000302:isolation & purification* / Q000652:urine
D000911:Antibodies, Monoclonal; D014361:Trypsin Inhibitors