A model system for in vivo gene transfer into the central nervous system using an adenoviral vector.
Previous methods of in vivo gene transfer to differentiated neurons of the adult mammalian brain have been inefficient and associated with technical problems. We have therefore developed a model system of direct gene transfer using a replication-defective adenoviral vector containing a beta-galactosidase gene to transduce brain neurons. Following injection of purified high titre recombinant adenovirus into the caudate putamen of seven week old mice, lacZ activity was evident in neural components of the central nervous system (CNS) for at least 8 weeks post infection. The efficiency of adenoviral gene transfer was very high compared to other techniques, suggesting an attractive and efficient alternative for neuronal gene transfer in vivo.
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0680.
B L
BL
Davidson
E D
ED
Allen
K F
KF
Kozarsky
J M
JM
Wilson
B J
BJ
Roessler
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D002421:Caudate Nucleus / Q000166:cytology / Q000648:ultrastructure; D003587:Cytomegalovirus / Q000235:genetics; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007150:Immunohistochemistry; D051379:Mice; D008809:Mice, Inbred C3H; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D011401:Promoter Regions, Genetic; D011699:Putamen / Q000166:cytology / Q000648:ultrastructure; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*
D001616:beta-Galactosidase