Microinjection of an nm23 specific antibody inhibits cell division in rat embryo fibroblasts.
Expression of the nm23/NDP-K gene correlates with reduced metastasis in some tumors and with increased proliferation in both nontransformed and transformed cells in culture. Decreased nm23/NDP-K expression results in mitotic arrest in neuroblasts of developing Drosophila. In order to better understand the biological role(s) of nm23 in non-transformed cells, an nm23-specific antibody was introduced into rat embryo fibroblasts and effects on DNA synthesis and cell cycle progression were analyzed. Microinjection of the nm23 antibody inhibited cell division with no apparent effect on DNA synthesis. Control experiments revealed that the survival of cells injected with the nm23 antibody was similar to that of control antibody injected cells in the absence of cell division. These results suggest that in mammalian fibroblasts, as in Drosophila, nm23 expression may be necessary for progression through the cell cycle.
Department of Medicine, University of California, San Diego, La Jolla 92093.
S M
SM
Sorscher
P
P
Steeg
J R
JR
Feramisco
C
C
Buckmaster
G R
GR
Boss
J
J
Meinkoth
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology*; D016590:Aphidicolin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004261:DNA Replication; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D007532:Isoleucine / Q000494:pharmacology; D008845:Microinjections; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase / Q000276:immunology / Q000502:physiology; D051381:Rats; D014157:Transcription Factors / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*
D000906:Antibodies; D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; D007532:Isoleucine; D016590:Aphidicolin; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins