Calmodulin-dependent phosphorylation and dephosphorylation during fertilization in Dictyostelium discoideum.
Many calmodulin-dependent biological phenomena are regulated by calmodulin-dependent phosphorylation and dephosphorylation. In Dictyostelium discoideum, cell and pronuclear fusion during fertilization are both calmodulin-dependent biomembrane fusion events. Calmodulin-dependent phosphorylation and dephosphorylation activity was present in sexually developing D. discoideum cells suggesting it has a role during fertilization. In support of this, a discrete calmodulin-independent kinase was present only during cell and pronuclear fusion.
Lydan
M A
MA
University of Toronto, Department of Zoology, Erindale College, Mississauga, Ontario, Canada.
O'Day
D H
DH
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D004023:Dictyostelium / Q000201:enzymology / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005306:Fertilization; D007700:Kinetics; D008561:Membrane Fusion; D008970:Molecular Weight; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D013997:Time Factors
D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002118:Calcium