Quality of platelet concentrates irradiated with UVB light: effect of UV dose and dose rate on glycocalicin release and correlation with other markers of the platelet storage lesion.
The amount of membrane-associated glycoprotein Ib in platelet concentrates (PCs) irradiated with a high dose of UVB light has been shown to be significantly reduced after 48 h storage. We recently corroborated this finding when we noted an increase in the supernatant levels of glycocalicin (GC, a major segment of glycoprotein Ib) in UVB-treated PCs during storage. The aim of the present study was to determine whether GC release was related to both the UV dose and the rate of dose delivery. Plateletpheresis concentrates obtained from five donors were pooled and split into five equal parts. Four of these were treated with 7500 and 15,000 mJ/cm2 UVB using two prototype UV sources with differing rates of dose delivery; namely, Baxter (BAT) and British Aerospace (BAC) cabinets, with the latter having the slower rate of delivery. On days 1 and 5 of storage, GC levels in the supernatants of PCs were determined by ELISA. Moreover, the following parameters were also assessed: platelet and WBC count; hypotonic shock response (HSR) and platelet aggregation response to ADP, ADP+collagen, ADP+arachidonic acid and ristocetin; pH; supernatant levels of lactate, glucose, von Willebrand factor (vWf) and beta-thromboglobulin (beta TG). The results revealed an association of GC release with UVB dose using both UV sources, although this was more apparent in the BAC system, in which glycocalicin release at day 5 of storage was as follows (microgram/ml, mean +/- SD): 4.8 +/- 0.3 and 9.5 +/- 3.6 at 7500 and 15,000 mJ/cm2 respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Edinburgh & S.E. Scotland Blood Transfusion Service, Department of Transfusion Medicine, Royal Infirmary of Edinburgh, U.K.
H
H
Bessos
W G
WG
Murphy
A
A
Robertson
M
M
Vickers
M J
MJ
Seghatchian
N P
NP
Tandy
M
M
Cutts
D H
DH
Pamphilon
D001792:Blood Platelets / Q000528:radiation effects*; D002462:Cell Membrane / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007773:Lactates / Q000032:analysis; D019344:Lactic Acid; D010979:Platelet Function Tests; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D013997:Time Factors; D014466:Ultraviolet Rays; D001620:beta-Thromboglobulin / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis
D007773:Lactates; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins; D001620:beta-Thromboglobulin; C023592:glycocalicin; D014841:von Willebrand Factor; D019344:Lactic Acid