Immunological characterisation of the lichen planus infiltrate using monoclonal antibodies.
The immunological typing of cell populations in the skin biopsies of 20 cases of histologically proven lichen planus was done using monoclonal antibodies. The Avidin-Biotin-Complex immunoperoxidase technique was used. A predominance of T-helper/inducer cells in the dermal infiltrate was observed by a semiquantitative analysis. T-suppressor/cytotoxic cells and Langerhans cells were increased compared to eczema lesions. A comparison of the staining of Langerhans cells using monoclonal antibodies and the S100 protein stain showed that more Langerhans cells could be identified with the CD1 monoclonal antibody. The type of infiltrate did not correlate with the duration of disease. Direct immunofluorescence staining demonstrated fibrinogen at the dermo-epidermal junction and/or colloid bodies in the upper dermis. The immunophenotyping and immunofluorescence pattern in local cases of lichen planus are similar to previous reports from other countries. An interesting finding was the unexplained disproportionate predominance of Indians in the study cohort.
National Skin Centre, Singapore.
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Tan
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Tham
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping; D007223:Infant; D007801:Langerhans Cells / Q000276:immunology; D008010:Lichen Planus / Q000208:ethnology / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal