Gas chromatographic assay of vigabatrin enantiomers in plasma.
A rapid and specific gas chromatographic method has been developed for the determination of plasma R(-)- and S(+)-vigabatrin concentrations. The method involves a double derivatisation step, chromatography on a megabore Chirasil-Val capillary column and thermionic specific detection. Concentrations in the range 1.0-200 micrograms/ml can be measured for R(-)-vigabatrin, and 0.5-100 micrograms/ml for S(+)-vigabatrin, using 250 microliters of plasma. The assay is suitable for pharmacokinetic studies and routine therapeutic drug monitoring in humans.
Schramm
T M
TM
Department of Medicine, University of Queensland, Royal Brisbane Hospital, Herston, Australia.
McKinnon
G E
GE
Eadie
M J
MJ
D000614:Aminocaproates / Q000097:blood*; D000927:Anticonvulsants / Q000097:blood*; D002849:Chromatography, Gas; D004827:Epilepsy / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D012015:Reference Standards; D013237:Stereoisomerism; D020888:Vigabatrin
D000614:Aminocaproates; D000927:Anticonvulsants; D007202:Indicators and Reagents; D020888:Vigabatrin