A biologic basis for altered host defenses in surgically infected abscesses.
OBJECTIVE
This study determines whether there are any differences in several immunologic parameters in circulating peripheral blood leukocytes, serum, and plasma compared with pus leukocytes, and supernatant of various types of abscess.


BACKGROUND
Although there have been reports of high lysozyme levels and low complement levels within pus, there has been no systemic comparison of concentrations of these substances and others within pus compared with those within peripheral blood.


METHODS
Peripheral blood and abscess pus were collected from 31 patients with abscesses and percent and mean channel of monocytes expressing complement receptor and major histocompatibility antigen HLA-DR, the percent and mean channel of polymorphonuclear leukocytes (PMN) expressing complement receptor, lysozyme level, and levels of total hemolytic complement, iC3b, C5b-9, and immunoglobulins were measured within both pus and peripheral blood.


RESULTS
Percent of monocytes expressing HLA-DR and percent of monocytes and PMN expressing complement receptor, total hemolytic complement, and IgM were reduced within pus compared with peripheral blood, whereas the mean channel of monocytes expressing HLA-DR and the mean channel of PMNs expressing CR3, C5b-9, and lysozyme were increased in pus.


CONCLUSIONS
There are marked differences in immunologic parameters measured within pus of abscesses versus that seen in peripheral blood.
Galandiuk
S
S
Department of Surgery, University of Louisville School of Medicine, Kentucky.
Appel
S H
SH
Polk
H C
HC
D000005:Abdomen; D000038:Abscess / Q000097:blood / Q000276:immunology* / Q000473:pathology; D003179:Complement C3b / Q000032:analysis; D015941:Complement Hemolytic Activity Assay; D015938:Complement Membrane Attack Complex / Q000032:analysis; D004322:Drainage; D016724:Empyema, Pleural / Q000097:blood / Q000276:immunology / Q000473:pathology; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood; D007962:Leukocytes / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000032:analysis; D009113:Muramidase / Q000032:analysis / Q000097:blood; D011446:Prospective Studies; D013481:Superoxides / Q000032:analysis / Q000097:blood; D013492:Suppuration / Q000097:blood / Q000276:immunology / Q000473:pathology
D015938:Complement Membrane Attack Complex; D006684:HLA-DR Antigens; D007075:Immunoglobulin M; D016177:Macrophage-1 Antigen; D013481:Superoxides; D003179:Complement C3b; D009113:Muramidase