Permeation of superoxide anion through the bilayer of vesicles of a synthetic amphiphile.
Large unilamellar vesicles, prepared with dioctadecyldimethylammonium chloride, entrap nitroblue tetrazolium. Addition of solid KO2, or production of superoxide anion by riboflavin photolysis, to nitroblue tetrazolium-containing dioctadecyldimethylammonium vesicles results in the formation of monoformazan above the phase-transition temperature of the bilayer. Below the phase-transition temperature the yield of monoformazan is negligible. These results demonstrate that superoxide anion permeates vesicles above the phase-transition temperature of the bilayer.
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
L F
LF
Gomes
I M
IM
Cuccovia
H
H
Chaimovich
D H
DH
Barbieri
M J
MJ
Politi
D000838:Anions / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008823:Micelles; D010782:Photolysis; D000644:Quaternary Ammonium Compounds; D013481:Superoxides / Q000737:chemistry*; D013696:Temperature
D000838:Anions; D008051:Lipid Bilayers; D008823:Micelles; D000644:Quaternary Ammonium Compounds; D013481:Superoxides; C015831:dimethyldioctadecylammonium