Inhibition of appearance of pH-dependent virus-cell fusion by neutralizing monoclonal antibodies to transmissible gastroenteritis virus.
Five MAbs, which showed neutralizing (NT) activity and recognized S (previously designated E2) glycoprotein of TGE virus TO-163, inhibited cell fusion by TGE virus in IB-RS-2 cells.  Other S-specific MAbs with no NT activity did not inhibit the cell fusion. The results indicate that a neutralization epitope is same or very close to a fusion active site. The kinetics of membrane fusion following a reduction in pH were also investigated. The cell fusion appeared between 2 and 6 hr after the binding medium was added.
Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
E
E
Honda
J
J
Okada
K
K
Okazaki
Y
Y
Kono
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002460:Cell Line; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008561:Membrane Fusion / Q000187:drug effects; D009500:Neutralization Tests; D005760:Transmissible gastroenteritis virus / Q000187:drug effects / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal