Sodium nitroprusside and neurostimulation cause increased levels of cyclic guanosine monophosphate and not cyclic adenosine monophosphate during canine penile erection.
We investigated the changes of cGMP and cAMP during penile erection induced by direct electrical stimulation of the pelvic nerve or sodium nitroprusside administration. Immediately after penile erection, the samples were put into liquid nitrogen and cyclic nucleotide content was measured. The results of these investigation suggest that both pelvic nerve stimulation and sodium nitroprusside induced penile tumescence. The total content of cGMP and not cAMP were significantly higher in treated samples as compared to control. SDS-polyacrylamide gel electrophoresis revealed several differences after treatment compared to untreated samples. This study demonstrated that the total content of cGMP and not cAMP increased after pelvic nerve stimulation and sodium nitroprusside treatment in penile tissue.
Dahiya
R
R
Department of Urology, University of California School of Medicine, San Francisco.
Trigo-Rocha
F
F
Brock
G
G
Narayan
P
P
Lue
T F
TF
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D004285:Dogs; D004558:Electric Stimulation; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology; D010410:Penile Erection / Q000187:drug effects
D009599:Nitroprusside; D000242:Cyclic AMP; D006152:Cyclic GMP