Cytotoxicity testing of two CAPD dialysis fluids in a model system of quiescent fibroblasts.
Indications of some bio-incompatibility of fluids used for continuous ambulatory peritoneal dialysis (CAPD) have been found previously. We have tested cytotoxicity of an frequently used dialysis fluid, Dianeal, and a new bicarbonate-based dialysis fluid with a pH of 7.4, called 87b, in a model system of quiescent fibroblasts. The ATP level of the cells and release of lactate dehydrogenase was followed during incubation in the dialysis fluids. A marked cytotoxicity of Dianeal was found mainly due to a low pH (5.0-5.5) and to a minor degree because of the lack of potassium and glutamine. 87b was less cytotoxic but it also lacked potassium and glutamine.
Kristensen
S R
SR
Department of Clinical Chemistry, Odense University Hospital, Denmark.
Pedersen
F B
FB
D000255:Adenosine Triphosphate / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015314:Dialysis Solutions / Q000009:adverse effects* / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008954:Models, Biological; D009994:Osmolar Concentration; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects*
D015314:Dialysis Solutions; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase