The detection of CR1 mRNA and CR1 antigen identifies the presence of immature podocytes in a case of Wilms' tumor (nephroblastoma).
Wilms' tumors (nephroblastomas) are heterogeneous tumors consisting of proliferating epithelial cells in glomeruloid bodies and tubule formations and of proliferating blastemal cells. Using a specific CR1 35S-labeled cDNA probe and CR1 antibodies, CR1 mRNA and CR1 antigen-expressing cells were detected in glomeruloid bodies in one case of Wilm's tumor. The concomitant expression of CR1 mRNA and CR1 antigen and the high level of CR1 mRNA expression that characterize podocytes at an early stage of glomerular maturation in the normal human fetal kidneys indicates that glomeruloid bodies are composed of immature podocytes.
INSERM U 28, Hôpital Broussais, France.
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MD
Appay
M
M
Kazatchkine
D
D
Droz
P
P
Bobe
J
J
Bariety
D015342:DNA Probes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007678:Kidney Glomerulus / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D017463:Receptors, Complement 3b / Q000032:analysis* / Q000235:genetics; D009396:Wilms Tumor / Q000235:genetics / Q000378:metabolism*
D015342:DNA Probes; D012333:RNA, Messenger; D017463:Receptors, Complement 3b