The colorimetric assay for swine lymphocyte blastogenesis.
The colorimetric assay used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was compared with the 3H-thymidine incorporation assay in swine lymphocyte blastogenesis induced by phytohemagglutinin-P (PHA) and concanavalin A (ConA). The correlation coefficients were 0.70 (n = 18, p < 0.01) in PHA and 0.82 (n = 18, p < 0.01) in ConA. The maximal responses were observed in cultures of 2.0 x 10(6) cells/ml with the final concentration of 2.5-10.0 micrograms/ml in PHA or 2.5-5.0 micrograms/ml in ConA for 72 hr. The stimulation index of peripheral blood lymphocytes from clinically healthy pigs aged 6 months were 1.77 +/- 0.10 (n = 10) in PHA and 1.96 +/- 0.18 (n = 10) in ConA, respectively.
Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Japan.
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D000818:Animals; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D003208:Concanavalin A; D004247:DNA / Q000096:biosynthesis; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D010835:Phytohemagglutinins; D011865:Radioisotope Dilution Technique; D013552:Swine / Q000276:immunology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D004396:Coloring Agents; D010835:Phytohemagglutinins; D013778:Tetrazolium Salts; D013844:Thiazoles; D014316:Tritium; D003208:Concanavalin A; D004247:DNA; C022616:thiazolyl blue; D013936:Thymidine