Differences in the subcellular localization of calreticulin and organellar Ca(2+)-ATPase in neurons.
It has become clear that calcium is an important mediator in the transduction of signals due to ligand binding to cell surface receptors. Cytosolic calcium is typically maintained at low levels in both muscle and non-muscle cells and intracellular sequestering of calcium appears to be important in this process. The identification of intracellular calcium pools has been the subject of much recent study, and it has been proposed that such pools would contain three components: a calcium-activated pump or Ca(2+)-ATPase, a calcium channel such as the inositol trisphosphate receptor or ryanodine receptor, and a high-capacity calcium-binding protein such as calsequestrin or calreticulin. We report here on the localization of two components, the organellar Ca(2+)-ATPase (SERCA) and calreticulin, in neuronal tissues. Using immunofluorescence and subcellular fractionation, we have found that for the most part, these two proteins do not co-localize in neuron cell bodies, dendrites, or axons; but may co-localize at the axon terminal.
Johnson
R J
RJ
Biochemistry Department, Boston University School of Medicine, MA 02118.
Pyun
H Y
HY
Lytton
J
J
Fine
R E
RE
D000818:Animals; D001369:Axons / Q000737:chemistry; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000032:analysis*; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D037282:Calreticulin; D002417:Cattle; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000032:analysis*; D008253:Macaca mulatta; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D009900:Optic Nerve / Q000166:cytology / Q000201:enzymology; D011689:Purkinje Cells / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D013347:Subcellular Fractions / Q000737:chemistry*; D016708:Synaptophysin / Q000032:analysis; D013574:Synaptosomes / Q000201:enzymology
D002135:Calcium-Binding Proteins; D037282:Calreticulin; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D016708:Synaptophysin; D000252:Calcium-Transporting ATPases; D002118:Calcium