P element-flanked inserts at the yellow locus of Drosophila melanogaster strains.
Large inserts have been found to appear within the yellow (y) locus of several unstable mutant lines derived from a parental strain (y2 sc+ w(aG)) of Drosophila melanogaster. The inserts of the individual mutant lines correspond roughly to a series of multimers (4.1, 7.1, 14.3 and 28.9 kbp, respectively) and are always located at the same position, somewhat upstream of the y gene transcription start site. Each of them begins and ends with a 1-kbp sequence which shares restriction sites with P elements. One of these inserts (14.3 kbp) has been partially sequenced (1270 bp from the 5' end). The first 1185 bp match exactly the sequence of an internally deleted P element. The remaining part of the sequence, on the other hand, is unrelated to P elements. It is assumed that large DNA sequences may be captured by the flanking P-element fragments and inserted into new chromosomal sites.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
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B
Kroczyńska
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Lobanov
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OP
Samarina
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D004247:DNA