Characterization of peptide YY receptors in rabbit colonic mucosa.
Quantitative receptor autoradiography localized a high-affinity binding site for 125I-peptide YY to the mucosa in rabbit distal colon. Scatchard binding analysis revealed a single-affinity binding site (KD = 0.29 nM) with binding specificity similar to other peptide YY-preferring receptors (peptide YY > or = neuropeptide Y >> pancreatic polypeptide). Radioligand binding studies using colonic mucosal membranes confirmed high-affinity peptide YY binding sites (KD = 0.26 nM) with time, temperature, and protein dependence, as well as saturability characteristic of receptor-ligand binding. Selective peptide analogues showed a subpopulation of these 125I-peptide YY binding sites to resemble the Y1-type neuropeptide Y receptor. Peptide YY may exert local antisecretory effects on the colonic epithelium via these binding sites.
Duke University Medical Center, Durham, NC 27705.
P J
PJ
Mannon
E J
EJ
Hernandez
S J
SJ
Mervin
S R
SR
Vigna
I L
IL
Taylor
D000818:Animals; D001345:Autoradiography; D003106:Colon / Q000737:chemistry; D003720:Densitometry; D005260:Female; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000737:chemistry*; D007457:Iodine Radioisotopes; D019894:Peptide YY; D010455:Peptides / Q000378:metabolism*; D011817:Rabbits; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000032:analysis; D011964:Receptors, Gastrointestinal Hormone
D005768:Gastrointestinal Hormones; D007457:Iodine Radioisotopes; D010455:Peptides; D011956:Receptors, Cell Surface; D011964:Receptors, Gastrointestinal Hormone; C082172:peptide YY receptor; D019894:Peptide YY