Identification of novel members of G-protein coupled receptor superfamily expressed in bovine taste tissue.
Using reverse transcription-polymerase chain reaction (RT-PCR) and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Physiological functions of these receptors in taste cells are discussed.
Matsuoka
I
I
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Mori
T
T
Aoki
J
J
Sato
T
T
Kurihara
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D004247:DNA; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D011984:Sensory Receptor Cells / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013649:Taste / Q000502:physiology*; D014059:Tongue / Q000378:metabolism* / Q000502:physiology
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D004247:DNA; D019204:GTP-Binding Proteins