Sequence analysis of closely related retrotransposon families from fission yeast.
Two families of retrotransposons, Tf1 and Tf2, have been isolated from the fission yeast, Schizosaccharomyces pombe. We report here the nucleotide (nt) sequence of a Tf2 element, the only retrotransposon family known from the commonly used laboratory strains, 972 and 975, and their derivatives. The total nt sequence of Tf2 was derived from the complete sequence of the coding region and 3' long terminal repeat (LTR) of randomly cloned element Tf2-1, and from a full 5' LTR and approximately one-third of the open reading frame (ORF) of Tf2-43, a Tf2 element found in the head-to-head orientation adjacent to the Sz. pombe rpb6 gene. The two Tf2 sequences are nearly identical and both of them contain a single ORF encoding a protein with regions of sequence similar to protease, reverse transcriptase, RNase H (RH) and integrase from other retrotransposons and retroviruses. Sequence comparisons between Tf1 and Tf2 indicate an extreme divergence of the putative capsid protein-encoding regions of these two elements, as well as divergence of a segment of the LTR, but otherwise virtually identical sequence.
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
D C
DC
Weaver
G V
GV
Shpakovski
E
E
Caputo
H L
HL
Levin
J D
JD
Boeke
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid; D004251:DNA Transposable Elements / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016366:Open Reading Frames; D012331:RNA, Fungal / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics
D004251:DNA Transposable Elements; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D012331:RNA, Fungal; D029702:Schizosaccharomyces pombe Proteins; C083145:Tf2-6 protein, S pombe; D014157:Transcription Factors; C422562:mitochondrial transcription factor A