Verification of the position of the phosphate group in some synthetic phosphopeptides by fast-atom bombardment and tandem mass spectrometry.
Some synthetically obtained linear and cyclic phosphopeptides of low molecular weight have been studied by fast-atom bombardment and tandem mass spectrometry to verify the position of the phosphate group in these compounds. Based upon the occurrence/non-occurrence of loss of phosphoric acid from low abundance fragment ions induced by low- and high-energy collisions with target gases, it is shown that the position of the phosphate group in the phosphopeptides studied can be determined unequivocally.
Institute of Mass Spectrometry, University of Amsterdam, The Netherlands.
A A
AA
Nijenhuis
R H
RH
Fokkens
N M
NM
Nibbering
A H
AH
van Oijen
H B
HB
de Bont
R M
RM
Liskamp
J H
JH
van Boom
D000595:Amino Acid Sequence; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010710:Phosphates / Q000032:analysis*; D010748:Phosphopeptides / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D010710:Phosphates; D010748:Phosphopeptides