Expression of the cholecystokinin gene in organotypic slice cultures of immature rat somatosensory cortex.
The preprocholecystokinin gene is expressed in a subpopulation of cortical interneurons containing gamma-aminobutyric acid. Slices of neonatal rat cortex were cultivated for 12 +/- 2 days and examined for the presence and distribution of these neurons by in situ hybridization and immunocytochemistry. Like in situ, two layers of preprocholecystokinin-mRNA-expressing cells were present. Immunopositive fibers formed a dense network and established symmetric contacts on dendritic shafts and spines. It is concluded that cholecystokinin-expressing interneurons survive in cultured slices of rat cerebral cortex. These organotypic cultures may be useful to study the cellular interactions which regulate neuronal cholecystokinin expression.
Olenik
C
C
Institute of Pharmacology, University of Freiburg, FRG.
Heimrich
B
B
Meyer
D K
DK
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002470:Cell Survival; D002766:Cholecystokinin / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression; D007395:Interneurons / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013003:Somatosensory Cortex / Q000378:metabolism*
D011498:Protein Precursors; D012333:RNA, Messenger; C042082:preprocholecystokinin; D002766:Cholecystokinin