In vivo accumulation of sodium pump inhibitor by normal and uremic erythrocytes.
A uremic toxin fraction (fraction 2-3-1) in the middle molecular mass range (300-2000 Da) containing a sodium potassium inhibitor pump was studied. As fraction 2-3-1 was known to be present in minute quantities in urine and plasma, erythrocyte lysates were used as an alternative source. Results show that fraction 2-3-1 is more abundant in erythrocytes than in normal urine. In addition, the fraction is present in a greater quantity in uremic erythrocytes than in normal blood cells. It is concluded that fraction 2-3-1 is concentrated at the erythrocyte level in uremic patients.
Biophysics Laboratory, Faculty of Pharmacy, Marseille, France.
P
P
Gallice
E
E
Lai
P
P
Brunet
Y
Y
Berland
R
R
Elsen
A
A
Crevat
D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D014118:Toxins, Biological / Q000097:blood* / Q000494:pharmacology; D014511:Uremia / Q000097:blood*
D014118:Toxins, Biological; C026865:uremia middle molecule toxins; D000254:Sodium-Potassium-Exchanging ATPase