Erythrocyte alterations correlate with CCl4 and biliary obstruction-induced liver damage in the rat.
Erythrocyte and hepatocyte plasma membranes derived from CCl4-cirrhotic and bile duct ligated rats were studied. Six groups of animals were used: Group 1 received CCl4 for 8 weeks. Animals in group 2 received CCl4 for 12 weeks. Group 3 consisted in animals that received CCl4 for 8 weeks and then only vehicle for 4 weeks more. Group 4 was the control which received only vehicle. In group 5, obstructive jaundice was induced by bile duct ligation (BDL); animals in group 6 were operated without ligation of the bile duct (sham operated). The activity of Na+/K+ and Ca2+ dependent ATPases, as well as the cholesterol/phospholipid ratio (CH/PL) were determined in both, erythrocyte and hepatocyte plasma membranes. In the CCl4 groups, Na+/K+ and Ca(2+)-ATPases activity was decreased about 60-70% while the CH/PL ratio was increased significatively in both, erythrocyte and hepatocyte plasma membranes. Discontinuation of CCl4 treatment reverted the enzymatic and lipidic alterations to normal in both cell types. ATPases activity and CH/PL ratio in erythrocyte resembled to those of hepatocytes in BDL rats. A strong correlation (p < 0.005) was found when erythrocyte ATPases activity and CH/PL ratio were compared with those of hepatocytes. Moreover, histopathological analysis of liver sections correlated well with both, erythrocyte and hepatocyte biochemical findings. We concluded that determination of ATPases activity and CH/PL ratio in erythrocyte membranes could be a simple, safe and useful marker of cell liver damage in CCl4-induced liver cirrhosis and biliary obstruction in the rat.
Departamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados del I.P.N., México, D.F.
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P
Muriel
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L
Favari
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C
Soto
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002251:Carbon Tetrachloride; D002462:Cell Membrane / Q000378:metabolism; D002779:Cholestasis / Q000150:complications / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000097:blood* / Q000209:etiology / Q000473:pathology; D008297:Male; D008563:Membrane Lipids / Q000097:blood / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D008563:Membrane Lipids; D002251:Carbon Tetrachloride; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase