Change in transport activities of vacuoles of the yeast Yarrowia lipolytica during its growth on glucose.
Vacuoles were isolated from Yarrowia lipolytica yeast cells taken at various growth phases under carbon or nitrogen limitation. Vacuoles from the cells at the logarithmic growth phase showed a high activity of vacuolar H(+)-ATPase (0.9-1.1 U/mg protein) and efficiently generated chemical proton gradient and membrane potential across the tonoplast. Ca(2+)- and citrate transport were found to be maximal at this growth phase. At growth retardation and then in the stationary phase all the parameters studied decreased irrespective of the method of growth limitation. The citrate-transporting activity of vacuoles completely disappeared at growth retardation, also irrespective of the limitation method and irrespective of whether yeast cells overproduced citrate in the culture medium. The citrate-transporting system of Y. lipolytica vacuolar membrane is concluded not to be involved in citrate efflux and this efflux is probably performed by the plasmalemma transport system.
Laboratory of Biochemistry of Membrane Processes, Russian Academy of Sciences, Pushchino, Moscow Region.
T V
TV
Kulakovskaya
R N
RN
Matyashova
N V
NV
Shishkanova
T V
TV
Finogenova
L A
LA
Okorokov
D000251:Adenosine Triphosphatases / Q000032:analysis; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002474:Cell-Free System; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D008361:Mannosidases / Q000032:analysis; D008564:Membrane Potentials; D011522:Protons; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013347:Subcellular Fractions; D014617:Vacuoles / Q000378:metabolism*; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism*; D043323:alpha-Mannosidase
D002951:Citrates; D011522:Protons; D000644:Quaternary Ammonium Compounds; D019343:Citric Acid; D008361:Mannosidases; D043323:alpha-Mannosidase; D000251:Adenosine Triphosphatases; D005947:Glucose; D002118:Calcium