Reproducibility and quantitation of matrix-assisted laser desorption ionization mass spectrometry: effects of nitrocellulose on peptide ion yields.
We have observed that using nitrocellulose in sample preparation for matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry increases the yield of peptide [M+H]+ ions. Addition of nitrocellulose also provides improvement in sample-to-sample reproducibility of MALDI ion yield and improves the precision of peptide quantitation. Mass spectrometry and optical microscopy results suggest that the nitrocellulose modifies the crystallization of matrix/analyte solution to allow more even coverage over the sample surface.
Texas A & M University, Department of Chemistry, College Station 77840.
L M
LM
Preston
K K
KK
Murray
D H
DH
Russell
D000595:Amino Acid Sequence; D003101:Collodion / Q000737:chemistry*; D003460:Crystallization; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*
D010455:Peptides; D003101:Collodion