Subtyping of Neisseria gonorrhoeae auxotype-serovar groups by pulsed-field gel electrophoresis.
Genomic DNA from 48 Neisseria gonorrhoeae isolates was digested with low-frequency cleavage (LFC) endonucleases (SpeI and NheI) and analysed by contour-clamped homogeneous electric fields electrophoresis (CHEF). The restriction patterns generated were reproducible, stable, easy to read and offer a more practical approach than restriction endonuclease analysis (REA) with high-frequency cleavage (HFC) endonucleases. Strains sharing common auxotypes and serovars could be differentiated and correlation with auxotype/serovar (A/S) classes was demonstrated for some, but not all strains. The strains were distributed into 18 A/S classes and 38 SpeI and 40 NheI restriction patterns. This greater discriminatory power of CHEF REA should allow subdivision of strains within common A/S classes.
Department of Microbiology, Faculty of Medicine, National University of Singapore.
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D015373:Bacterial Typing Techniques; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics; D012703:Serotyping
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; C067043:endodeoxyribonuclease NheI; C056436:endodeoxyribonuclease SpeI; D015252:Deoxyribonucleases, Type II Site-Specific