Simian virus 40 small-t antigen binds two zinc ions.
Six cysteine residues of the simian virus 40 small-t antigen (small-t) are important for stability of the protein. Stability has been shown to be related to the ability of small-t to bind zinc ions in vitro. Purified small-t expressed either in bacteria or from baculovirus vectors binds two molecules of zinc per molecule of protein. Thus, small-t may resemble GAL4, which contains a Zn(II)2Cys6 binuclear cluster.
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611-3008.
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Turk
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Porras
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Rundell
D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
D000957:Antigens, Viral, Tumor; D011994:Recombinant Proteins; D015032:Zinc