Evaluation of urea and albumen as endogenous markers of dilution of equine bronchoalveolar lavage fluid.
The urea and albumen dilution techniques for standardising the variable concentrations of pulmonary epithelial lining fluid (PELF) in bronchoalveolar lavage fluid (BALF) samples were evaluated in horses. Both techniques proved satisfactory and were of equal accuracy. Albumen adjusted BALF cell counts were significantly higher than PELF cell counts. BALF from normal horses contained, on average, 0.4 per cent PELF (range 0.1 to 1.0 per cent), as determined by the urea dilution technique.
McGorum
B C
BC
Department of Veterinary, Clinical Studies, Royal (Dick) School of Veterinary Studies, Roslin, Midlothian.
Dixon
P M
PM
Halliwell
R E
RE
Irving
P
P
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology / Q000662:veterinary*; D002452:Cell Count; D006736:Horses; D007962:Leukocytes / Q000166:cytology; D012709:Serum Albumin / Q000032:analysis*; D014508:Urea / Q000032:analysis* / Q000097:blood
D015415:Biomarkers; D012709:Serum Albumin; D014508:Urea