Quantitative determination of non-ionic surfactants in protein samples, using ion-exchange guard columns.
Quantitative determination of Triton X-100 in buffered, organic modifier-containing solutions was performed by use of strong cation- and anion-exchange HPLC guard columns. Recovery was complete for Triton X-100 concentrations of 0.1%, 0.05%, 0.25% and 0.0125% (wt/wt) in 50 mM potassium phosphate buffer (pH 5.0 +/- 0.05, pH 4.0 +/- 0.05, pH 3.0 +/- 0.05 and pH 2.0 +/- 0.05) modified with 30% isopropanol. This procedure can be applied to recovery of other nonionic surfactant systems.
Supelco, Inc., Bellefonte, PA 16823-0048.
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D000818:Animals; D001709:Biotechnology; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003574:Cytochrome c Group / Q000302:isolation & purification; D003902:Detergents / Q000032:analysis; D005069:Evaluation Studies as Topic; D006454:Hemoglobins / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000032:analysis; D011506:Proteins / Q000302:isolation & purification*; D013501:Surface-Active Agents / Q000032:analysis*
D003574:Cytochrome c Group; D003902:Detergents; D006454:Hemoglobins; D011506:Proteins; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol