A common proviral integration region, fit-1, in T-cell tumors induced by myc-containing feline leukemia viruses.
Feline leukemia viruses carrying transduced v-myc genes (myc-FeLV) induce tumors of clonal origin, suggesting that activated myc alone is not sufficient for tumorigenesis. To investigate the hypothesis that insertional mutagenesis plays a role by activating genes which collaborate with v-myc, we looked for evidence of common proviral integration sites in these tumors. By inverse polymerase chain reaction we identified a 6-kb domain, designated fit-1, in which FeLV proviruses were inserted in four of nine (44%) T-cell tumors induced by myc-FeLV. The fit-1 locus was mapped to feline chromosome B2 and appears to be distinct from known oncogenes located on this chromosome. Fit-1 represents a novel common proviral integration region which may harbor a cellular gene which acts in concert with the myc gene in T-cell tumorigenesis.
Department of Veterinary Internal Medicine, University of Tokyo, Japan.
H
H
Tsujimoto
R
R
Fulton
K
K
Nishigaki
Y
Y
Matsumoto
A
A
Hasegawa
A
A
Tsujimoto
S
S
Cevario
S J
SJ
O'Brien
A
A
Terry
D
D
Onions
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002874:Chromosome Mapping; D016259:Genes, myc / Q000235:genetics*; D005256:Leukemia Virus, Feline / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics; D011533:Proviruses / Q000235:genetics*; D016662:Virus Integration / Q000235:genetics*