Evolutionarily conserved motifs and protein binding elements in the 5' region of the chromosomal protein HMG-14 gene.
Although the structure of several genes coding for chromosomal proteins HMG-14 and HMG-17 has been determined, the mechanisms regulating the expression of these genes has not yet been examined. Toward this goal, we have cloned and sequenced a fragment containing the first three exons and 956 bp upstream from the start of translation of the functional mouse HMG-14 gene. Comparison of this sequence to the known sequence of the human HMG-14 gene revealed the presence of five distinct blocks of high sequence identity flanking the start of transcription and the CAAT box. DNase I and mobility-shift analysis identified a DNA region, downstream from the start of transcription, which may be important for the formation of a stable protein-DNA complex. Affinity chromatography on columns containing oligonucleotides corresponding to this sequence indicates that this region is a protein binding site.
Lehn
D A
DA
Protein Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Bustin
M
M
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D005796:Genes; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid
D017931:DNA Primers; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins