In vitro effects of recombinant human interferon gamma on human mesothelioma cell lines.
Malignant mesothelioma is a tumor arising from serous surfaces and often related to asbestos exposure. Malignant mesothelioma is resistant to various forms of therapy. Radiotherapy, surgery or chemotherapy only slightly improve prognosis. IFN-gamma produces complete or partial responses in stage-I patients with malignant mesothelioma. The in vitro biological effect of IFN-gamma on malignant mesothelioma cells remains poorly elucidated. In the present study, 32 well-characterized human mesothelioma cell lines (HMCL) were treated with r-hu IFN-gamma at 4 doses and cell growth was determined by a colorimetric method (MTT assay). Among the 32 HMCLs tested, II exhibited significant cell-growth inhibition; 16 were insensitive to r-hu IFN-gamma, and 5 were slightly inhibited. The sensitive cell lines were strongly inhibited by r-hu IFN-gamma. Our results show that HMCL exhibit a large range of responses to r-hu IFN-gamma, some of which can be compared with those obtained in vivo in humans.
Laboratorie de Pathologie Cellulaire et Moléculaire de l'Environnement, INSERM U139, C.H.U. Henri Mondor, Créteil, France.
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L
Zeng
A
A
Buard
I
I
Monnet
C
C
Boutin
J
J
Fleury
L
L
Saint-Etienne
P
P
Brochard
J
J
Bignon
M C
MC
Jaurand
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D008654:Mesothelioma / Q000473:pathology* / Q000628:therapy; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D010997:Pleural Neoplasms / Q000473:pathology* / Q000628:therapy; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D007371:Interferon-gamma