A new method of RNA preparation for detection of hepatitis A virus in environmental samples by the polymerase chain reaction.
A new method based on RNA preparation from shellfish by polyethylene glycol (PEG) precipitation and trichloroacetic acid (TCA) extraction was developed for the detection of hepatitis A virus (HAV) by the polymerase chain reaction (PCR). The extraction provides high yield and the extracted RNA is undegraded. This method proved to be particularly useful for detection of RNA viruses from environmental samples by PCR.
Department of Marine Biology, Third Institute of Oceanography, Xiamen, People's Republic of China.
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D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000382:microbiology*; D011232:Chemical Precipitation; D004199:Disease Vectors; D006507:Hepatovirus / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011092:Polyethylene Glycols; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification*; D014238:Trichloroacetic Acid; D014871:Water Microbiology
D012367:RNA, Viral; D011092:Polyethylene Glycols; D014238:Trichloroacetic Acid