Identification of 27-nor-3 alpha, 7 alpha, 12 alpha-trihydroxycoprostan-24-one apparently derived from 3 alpha, 7 alpha, 12 alpha-trihydroxy-24-oxocoprostanoic acid, a postulated intermediate of bile acid biosynthesis.
Incubation of [27-13C]-3 alpha, 7 alpha, 12 alpha-trihydroxycoprost-24-en-26-oic acid with rat liver homogenate followed by 13C-NMR analysis of the incubation product has resulted in the identification of [26-13C]-27-nor-3 alpha, 7 alpha, 12 alpha-trihydroxycoprostan-24-one, supporting the idea that the substrate has been metabolized into 3 alpha, 7 alpha, 12 alpha-trihydroxy-24-oxocoprostan-26-oic acid CoA derivative.
Yuri
M
M
Department of Chemistry, Tokyo Institute of Technology, Japan.
Tokumoto
M
M
Hara
N
N
Fujimoto
Y
Y
Kobayashi
N
N
Morisaki
M
M
D000818:Animals; D001647:Bile Acids and Salts / Q000096:biosynthesis* / Q000737:chemistry; D002777:Cholestanols / Q000032:analysis*; D002778:Cholestanones / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats
D001647:Bile Acids and Salts; D002777:Cholestanols; D002778:Cholestanones; C022069:3,7,12-trihydroxycholestan-26-oic acid; C083341:27-nor-3,7,12-trihydrocoprostan-24-one