Norepinephrine transporter mRNA is elevated in the locus coeruleus following short- and long-term desipramine treatment.
In situ hybridization for the norepinephrine transporter (NET) mRNA was performed in animals receiving short-term (2 days) and long-term (4 weeks) treatment with desipramine (DMI; 10 mg/kg, intraperitoneal). Following short-term and long-term DMI treatment, a significant (P < 0.05) increase in hybridization of 35S-labeled oligonucleotides to NET mRNA in the locus coeruleus was observed compared to that observed in vehicle-treated animals. The mechanism of this increase in transporter mRNA or its involvement in the therapeutic effects of anti-depressants remains to be determined.
Szot
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Geriatric Research, Education and Clinical Center (GRECC), Veterans Affairs Medical Center, Seattle, WA 98108.
Ashliegh
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EA
Kohen
R
R
Petrie
E
E
Dorsa
D M
DM
Veith
R
R
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D003891:Desipramine / Q000494:pharmacology*; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization; D008125:Locus Coeruleus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D050484:Norepinephrine Plasma Membrane Transport Proteins; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical; D013462:Sulfur Radioisotopes; D027981:Symporters
D002352:Carrier Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; C493226:Slc6a2 protein, rat; D013462:Sulfur Radioisotopes; D027981:Symporters; D003891:Desipramine; D009638:Norepinephrine