Evaluation of bovine fibrin sealant in the dog.
Fibrin sealant (human fibrinogen-bovine thrombin) is an effective biodegradable hemostatic agent. However, there is a risk of transmission of infectious viral disease. A new bovine fibrinogen-thrombin sealant (BFTS) was tested for tissue and immune responses in intrathoracic aorta graft in dogs. Intrathoracic aorta replacement was performed in three dogs with a porous Dacron graft presealed with BFTS. Dogs were immunized preoperatively with four dermal applications of BFTS at 9-day intervals. Cellular and humoral immunity to BFTS were determined with lymphocyte blastogenesis test and enzyme-lined immunosorbent assay, respectively. Dogs were necropsied 3 weeks after aortic replacement. Histopathological examination showed that all dogs had a mild inflammatory reaction to the BFTS sealed graft, as expected in response to an inert foreign body. Assay of cellular and humoral immunity to BFTS revealed a low lymphocyte response and a moderate immunoglobulin G (Ig G) response, with no evidence of immediate Ig E (type I) or delayed (type IV) hypersensitivity reaction. We conclude that BFTS causes no adverse tissue response or immunologic reaction when used as a hemostatic agent in the dog even after multiple applications of the material.
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
B M
BM
Bouvy
E
E
Rosin
K J
KJ
Frishmeyer
R R
RR
Dubielzig
R D
RD
Schultz
D000818:Animals; D001013:Aorta, Thoracic / Q000601:surgery*; D001807:Blood Vessel Prosthesis; D002417:Cattle; D004285:Dogs; D005069:Evaluation Studies as Topic; D015718:Fibrin Tissue Adhesive / Q000276:immunology*; D008297:Male; D012307:Risk Factors; D014777:Virus Diseases / Q000635:transmission
D015718:Fibrin Tissue Adhesive