Inhibition of herpes simplex virus replication by genistein, an inhibitor of protein-tyrosine kinase.
Genistein, an inhibitor of protein-tyrosine kinase, inhibited the replication of herpes simplex virus type 1 (HSV-1) at genistein concentrations of more than 25 microM, whereas the related compounds, which do not inhibit protein-tyrosine kinases, did not affect the replication of HSV-1. In the presence of genistein, the phosphorylation of tyrosine residues in specific viral polypeptides was markedly reduced. These results indicate that the phosphorylation of tyrosine residues in viral polypeptides may be essential for the replication of HSV-1.
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
Y
Y
Yura
H
H
Yoshida
M
M
Sato
D000818:Animals; D002451:Cell Compartmentation; D005455:Fluorescent Antibody Technique; D019833:Genistein; D007529:Isoflavones / Q000494:pharmacology*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development; D014443:Tyrosine / Q000378:metabolism; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000302:isolation & purification; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D007529:Isoflavones; D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; C040641:genistin; C083295:prunetin; D014443:Tyrosine; C004742:daidzein; D019833:Genistein; D011505:Protein-Tyrosine Kinases