Characterization of stimulatory surface molecular expression on avian T cells.
We have characterized a monoclonal antibody (AT389), produced by immunization of mice with 4-day-old avian Concanavalin A blasts, which recognizes a 38-40 kDa homodimer present on the surface of all chicken T cells. AT389 increased the spontaneous and IL-2-driven proliferation of 3-day-old T-cell blasts. This antibody, in synergy with IL-2, increased cellular proliferation and cell survival of T-cell blasts over time. Together, these data suggest that AT389 recognizes a surface protein on avian T cells which may be related to known stimulatory surface markers on mammalian T cells.
Department of Immunology and Microbiology, Wayne State University, Detroit, Michigan.
M H
MH
Kaplan
S
S
Alhasan
A
A
Rosenspire
R S
RS
Sundick
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D018106:CD28 Antigens; D017252:CD3 Complex / Q000032:analysis; D002460:Cell Line; D002645:Chickens; D005260:Female; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018106:CD28 Antigens; D017252:CD3 Complex