X-ray microanalysis of epithelial cells in culture.
X-ray microanalysis of cultured cells can be used to study ion transport properties of epithelial cells in vitro. A method was developed to culture a variety of epithelial and secretory cells (colon cancer cells, insulinoma cells, sweat gland epithelium, bovine mammary epithelium). The cells were grown on Formvar-film covered titanium grids, rinsed, frozen, and freeze-dried. The cultures of colon cancer and the bovine mammary epithelial cells appeared to contain two subpopulations of cells with different elemental composition. cAMP-mediated chloride secretion could be studied in the colon cancer cells and the cultured sweat gland epithelium: the cellular content of chloride decreased after stimulation with cAMP or with substances that increase the cellular level of cAMP.
Department of Human Anatomy, University of Uppsala, Sweden.
G M
GM
Roomans
A
A
von Euler
A C
AC
Mörk
E
E
Pålsgård
C
C
Vult von Steyern
D000818:Animals; D001692:Biological Transport; D002412:Cations / Q000032:analysis*; D002417:Cattle; D002478:Cells, Cultured; D002712:Chlorides / Q000032:analysis*; D046508:Culture Techniques / Q000379:methods; D000242:Cyclic AMP / Q000494:pharmacology; D004577:Electron Probe Microanalysis; D004848:Epithelium / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006801:Humans; D013545:Sweat Glands / Q000166:cytology / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002412:Cations; D002712:Chlorides; D000242:Cyclic AMP