Application of indirect ELISA for detection of bovine antibodies against vesicular stomatitis viruses.
Two indirect enzyme-linked immunosorbent assays (I-ELISAs) are described for the detection of bovine serum antibody to the New Jersey (NJ) and Indiana (IN) vesicular stomatitis viruses (VSV). Serum samples at a dilution of 1:200 were incubated with binary ethylenimine-inactivated VSV-NJ and VSV-IN type-specific antigens preadsorbed to microtiter plates. Bound antibodies were detected by a murine monoclonal antibody to bovine IgG1 conjugated with horseradish peroxidase. The performance of each I-ELISA in detecting homotypic and heterotypic antibodies to VSV-NJ and VSV-IN in sequential serum samples from calves experimentally infected with VSV-NJ or VSV-IN was evaluated. The I-ELISAs detected serotype-specific antibodies to either VSV-NJ or VSV-IN in calves infected with the homologous serotype. Homotypic but not heterotypic anti-VSV-NJ antibodies were first demonstrable by the VSV-NJ I-ELISA during the second week postinfection and remained at an elevated level for a period of 11 weeks, with a gradual decrease thereafter. Similar homotypic antibody profiles measured by the VSV-IN I-ELISA in calves inoculated with VSV-IN were observed. The performances of the I-ELISAs were compared using 1,495 microtiter serum neutralization (MTSN) test-negative bovine field sera collected from cattle in Canada (VS free) and 429 samples collected from cattle in the USA and Mexico (VS-epidemic and VS-endemic areas). The diagnostic specificities of the VSV-NJ and VSV-IN I-ELISAs for the Canadian samples relative to the MTSN test results were in the range of 99.8% and 99.7%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Afshar
A
A
Animal Diseases Research Institute, Agriculture Canada, Nepean, ON.
Dulac
G C
GC
Wright
P F
PF
Martin
D
D
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007196:Indiana; D009515:New Jersey; D013280:Stomatitis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D013997:Time Factors; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus; D018116:Vesiculovirus; D014777:Virus Diseases / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
D000914:Antibodies, Viral