Analysis of gamma delta+ T cells in peripheral blood of children with perinatal human immunodeficiency virus (HIV) infection.
The present study examined CD8 antigen expression and variable (V) gene segment usage by T cell receptor (TCR)-gamma delta+ lymphocytes in peripheral blood of symptomatic children with perinatal HIV infection. The relative number of gamma delta+, CD8+ T cells in most of the infected children was higher than that in uninfected children from HIV+ or HIV- mothers and correlated with the immunodeficiency status of the patients. Infected infants and children over 1 year old also showed an increased proportion of V delta 1-J delta 1+ T lymphocytes. CD8 expression on those cells was higher in infected than in uninfected infants and children. Sequence analysis of the delta gene rearrangement of the predominant V delta 1 family in peripheral blood of three HIV+ donors revealed extensive junctional diversity. These results suggest that the V delta skewing in the majority of HIV+ children reflects peripheral expansion of V delta 1-J delta 1+ T lymphocytes early in life, which might be involved in the mechanisms of HIV-induced immunodeficiency.
Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
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Kozbor
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Hyjek
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Wiaderkiewicz
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Kurzawski
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Lischner
D001483:Base Sequence; D017252:CD3 Complex / Q000276:immunology; D016516:CD4-CD8 Ratio; D002648:Child; D002675:Child, Preschool; D005434:Flow Cytometry; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor / Q000235:genetics; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis* / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D017252:CD3 Complex; D016692:Receptors, Antigen, T-Cell, gamma-delta