Interleukin-2 modulates calcium currents in dissociated hippocampal CA1 neurons.
Interleukin-2 and its receptors are present in hippocampal structures, particularly the pyramidal cell layer. In the present study, we examined the interaction between recombinant human interleukin-2 (rhIL-2) and the voltage-dependent calcium (Ca2+) current using the whole-cell patch-clamp technique. RhIL-2 depressed a fraction of the inward Ca2+ current in acutely dissociated guinea-pig hippocampal CA1 neurons. This depression is rapid, dose-dependent with 0.001-1.0 ng rhIL-2/10 microliters (6.8 x 10(-12) to 6.8 x 10(-9) M) and reversible in > 80% of the neurons. Heat-inactivated rhIL-2 had no effect. The application of anti-rhIL-2 monoclonal antibody inhibited the rhIL-2-induced depression of the Ca2+ current. The depression of the Ca2+ current by IL-2 may play a role in the induction and/or progression of neuropathological processes of immunological origin, and in the induction of neurological manifestations during IL-2 immunotherapy.
Plata-Salamán
C R
CR
School of Life and Health Sciences, University of Delaware, Newark 19716.
ffrench-Mullen
J M
JM
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects; D004594:Electrophysiology; D006168:Guinea Pigs; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D015640:Ion Channel Gating / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011712:Pyramidal Tracts / Q000166:cytology / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D007376:Interleukin-2; D011994:Recombinant Proteins