Amiloride-sensitive Na+ current in the granular duct cells of mouse mandibular glands.
Whole-cell patch clamp studies on dispersed cells from the intralobular ducts of mouse mandibular glands reveal the presence of an amiloride-sensitive Na(+)-selective conductance that is not found in the cells of the secretory endpieces. Since studies on ripped-off outside-out patches from the basolateral membranes of isolated intralobular ducts show that the membrane conductance is not altered by amiloride, it can be inferred that the Na+ conductance seen in whole-cell experiments is located in the luminal membrane. These studies thus provide evidence for the presence of a luminal Na+ conductance in salivary ducts and indicate that intralobular and extralobular ducts may handle Na+ in a similar manner.
Department of Physiology, University of Sydney, NSW, Australia.
A
A
Dinudom
J A
JA
Young
D I
DI
Cook
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D004553:Electric Conductivity; D008297:Male; D008536:Meglumine / Q000494:pharmacology; D051379:Mice; D012469:Salivary Glands / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology*
D015222:Sodium Channels; D008536:Meglumine; D000584:Amiloride