Determination of phloroglucinol in human plasma by gas chromatography-mass spectrometry.
A specific and sensitive method has been developed for the determination of phloroglucinol in plasma; it involves an optimized procedure for blood sampling designed to minimize the in vitro oxidation of the molecule, and gas chromatography-mass spectrometry after silylation of the compound. The method allowed a reliable determination of phloroglucinol in plasma. The precision and accuracy of the assay, reported as coefficients of variation, were below 15%. Using a plasma sample of 0.25 ml, the limit of quantitation was 5 ng/ml with a precision of 17.4%, which is sensitive enough for pharmacokinetic studies. Stability studies under different conditions revealed that ascorbic acid limits the degradation of phloroglucinol in plasma during storage at freezer temperatures.
Laboratoire de Chimie Analytique et de Spectrométrie de Masse, Faculté de Pharmacie, Clermont Ferrand, France.
C
C
Lartigue-Mattei
C
C
Lauro-Marty
M
M
Bastide
J A
JA
Berger
J L
JL
Chabard
E
E
Goutay
J M
JM
Aiache
D004355:Drug Stability; D004356:Drug Storage; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D010696:Phloroglucinol / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results
D010696:Phloroglucinol