Human herpesvirus-6-associated suppression of growth factor-induced macrophage maturation in human bone marrow cultures.
Cultures of marrow mononuclear cells were exposed to medium derived from cell cultures infected with several different strains of human herpesvirus-6 (HHV-6) immediately before the addition of either of two growth factors, granulocyte-macrophage colony-stimulating factor and interleukin-3. Exposure to any of the viral preparations suppressed the outgrowth of nonspecific esterase-positive adherent macrophages induced by the factors by more than 90%. The nonadherent cell populations in the infected cultures were numerically similar to those in uninfected control cultures, demonstrating the absence of a nonspecific cytotoxic effect of the viral materials. Infectious virus was not necessary for the macrophage outgrowth suppression. These findings suggest that HHV-6 either encodes or induces a soluble mediator or mediators that can interfere with the responses of bone marrow to growth factors and possibly block the normal differentiation of macrophages from marrow precursors.
Burd
E M
EM
Department of Pathology, Medical College of Wisconsin, Milwaukee 53226.
Knox
K K
KK
Carrigan
D R
DR
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D015654:Herpesvirus 6, Human / Q000472:pathogenicity*; D006801:Humans; D007377:Interleukin-3 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice
D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor