Introduction to recombinant-DNA technology.
Recombinant-DNA technology is now commonly used in virtually every aspect of the biological sciences. The purpose of this brief exposition is to provide an outline of the approaches used to identify genes, to isolate the gene of interest, to amplify the gene if necessary, and to clone genes. A short introduction to the principles of separating very large genes is provided, along with a description of an approach to propagating and cloning these large genes.
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis.
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D000818:Animals; D001709:Biotechnology / Q000379:methods*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005822:Genetic Vectors; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics
D004274:DNA, Recombinant; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes