Nuclease sensitivity and functional analysis of a maize histone H3 gene promoter.
A 1 kb region of a maize H3 histone gene promoter has been analysed at a structural and functional level. Micrococcal nuclease digestion of isolated nuclei showed that the promoter region is organized into nucleosomes but a zone extending over approximately one nucleosome (20 to 230 bp upstream of the TATA box) displays remarkable accessibility to digestion. Three DNase I-hypersensitive sites were found within this zone at the vicinity of consensus sequences, some of which are already known to act as cis elements. This promoter region is able to direct faithful expression of the GUS reporter gene in meristematic tissues of transgenic tobacco plants.
Brignon
P
P
Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, Strasbourg, France.
Lepetit
M
M
Gigot
C
C
Chaubet
N
N
D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA; D003850:Deoxyribonuclease I / Q000378:metabolism; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D006657:Histones / Q000235:genetics*; D008836:Micrococcal Nuclease / Q000378:metabolism; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics; D003313:Zea mays / Q000235:genetics*
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D004247:DNA; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease; D005966:Glucuronidase