Determination of tenoxicam in plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic technique with a lower detection limit for measuring tenoxicam plasma levels was standardized. Tenoxicam was extracted with dichloromethane from acidified plasma and the evaporated extracts were analysed on a reversed-phase column using a methanol-phosphate buffer mobile phase and setting ultraviolet detection at 355 nm. A reproducible calibration curve in the 5-2000 ng/ml range was obtained. The mean recovery of extraction was 98.99 +/- 4.3% and the detection limit was 5 ng/ml.
Múnera-Jaramillo
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MI
Clinical Pharmacology Unit, Corporación para Investigaciones Biológicas C.I.B., Medellin, Colombia.
Botero-Garcés
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D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000097:blood; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D012996:Solutions; D010894:Piroxicam; C032801:tenoxicam