3'-azido-3'-deoxythymidine (AZT) monophosphate: an inhibitor of exonucleolytic repair of AZT-terminated DNA.
A 3'-exonuclease(s) that excised 3'-azido-3'-deoxythymidine (AZT) monophosphate (AZTMP) from the 3' terminus of DNA was partially purified from two human cell lines. AZTMP inhibited the hydrolysis of AZTMP-terminated single-stranded and double-stranded DNA substrates. Thus, high levels of AZTMP might inhibit the exonuclease and trigger the toxicity of AZT by impairing the repair of AZTMP-terminated DNA.
Harrington
J A
JA
Wellcome Research Laboratories, Burroughs Wellcome Co., Research Triangle Park, North Carolina 27709.
Reardon
J E
JE
Spector
T
T
D002460:Cell Line; D004260:DNA Repair / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000187:drug effects; D054306:Dideoxynucleotides; D005092:Exonucleases / Q000037:antagonists & inhibitors*; D006801:Humans; D006868:Hydrolysis; D013942:Thymine Nucleotides / Q000494:pharmacology* / Q000633:toxicity; D015215:Zidovudine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity
D004277:DNA, Single-Stranded; D054306:Dideoxynucleotides; D013942:Thymine Nucleotides; C057287:3'-azido-3'-deoxythymidine 5'phosphate; D015215:Zidovudine; D005092:Exonucleases