A new, highly sensitive method for the detection and quantification of penicillin-binding proteins.
A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.
Galleni
M
M
Laboratoire d'Enzymologie, Université de Liège, Sart-Tilman, Belgium.
Lakaye
B
B
Lepage
S
S
Jamin
M
M
Thamm
I
I
Joris
B
B
Frère
J M
JM
D000192:Actinomycetales / Q000201:enzymology; D001407:Bacillus / Q000737:chemistry; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry; D019793:Fluorescein; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis*; D010397:Penicillanic Acid / Q000031:analogs & derivatives; D010400:Penicillin G / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology; D013388:Succinimides / Q000494:pharmacology
D001426:Bacterial Proteins; D002352:Carrier Proteins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D046915:Penicillin-Binding Proteins; D013388:Succinimides; D010397:Penicillanic Acid; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; C001426:N-hydroxysuccinimide; D010400:Penicillin G; C004408:aminopenicillanic acid; D019793:Fluorescein