Amplification mediated by polyomavirus large T antigen defective in replication.
The polyomavirus large T antigen promotes homologous recombination at high rates when expressed in rat cells carrying the viral replication origin and two repeats of viral DNA sequences stably integrated into the cellular genome. Recombination consists of both reciprocal and nonreciprocal events and is promoted by mutants defective in the initiation of viral DNA synthesis (L. St-Onge, L. Bouchard, and M. Bastin, J. Virol. 67:1788-1795, 1993). We have extended our studies to a rat cell line undergoing amplification of the viral insert. We show that large T antigen promotes amplification independently of its replicative function but that its origin-specific DNA binding activity is not sufficient to promote homologous recombination.
Department of Biochemistry, University of Sherbrooke, Quebec, Canada.
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St-Onge
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Bastin
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004260:DNA Repair; D004261:DNA Replication; D005814:Genes, Viral; D008957:Models, Genetic; D008961:Models, Structural; D008969:Molecular Sequence Data; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011995:Recombination, Genetic; D015183:Restriction Mapping; D017384:Sequence Deletion; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming