Cystic fibrosis--V. Does cystic fibrosis alter the values of dynamic parameters of erythrocyte membrane ghosts?
1. The dynamic properties of erythrocyte membranes in CF children have been investigated by means of fluorescence and ESR techniques. 2. It has been revealed that the apparent distance separating the membrane protein tryptophan and bound 1-anilino-8-naphthalenesulphonate (ANS) molecules is decreased in CF children which results in a significant increase of the maximum energy transfer efficiency. 3. The slight increase in the ratio hw/hs of maleimide bound to membrane protein-SH groups of erythrocytes in cystic fibrosis may ensue the lowered membrane protein immobilization in the plane of lipid bilayer, especially at the intrinsic, more slowly reacting thiol groups.
Department of Biophysics, University of Lódź, Poland.
H
H
Witas
C
C
Watała
K
K
Jedrychowska
W
W
Piasecki
D000817:Anilino Naphthalenesulfonates / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000097:blood*; D004161:Diphenylhexatriene / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy; D004735:Energy Transfer; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008301:Maleimides / Q000097:blood; D008565:Membrane Proteins / Q000097:blood / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000097:blood; D014364:Tryptophan / Q000097:blood
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008301:Maleimides; D008565:Membrane Proteins; D013438:Sulfhydryl Compounds; D004161:Diphenylhexatriene; C043592:maleimide; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan