[The effect of OCl- and Ag+ on active and passive calcium transport in the sarcoplasmic reticulum].
After hypochlorite (OCl-) treatment of the aortic smooth muscle sarcoplasmic reticulum (SR), the membrane microviscosity increases considerably in "bound" lipid regions in comparison with protein-free regions. OCl- induces the inhibition of active and the enhancement of passive calcium transport in SR. Treatment of SR vesicles with Ag+ and then with OCl- (but not in the reverse order) leads to the enhancement of the activating effect of OCl- on passive calcium release from the vesicles. It is concluded that the enhancement effect is due to the OCl(-)-induced increase in the passive permeability of the SR membrane for Ca2+ as a result of pore formation in the lipid bilayer.
Antipenko
A E
AE
Pechatnikova
E V
EV
Dizhe
G P
GP
Krasovskaia
I E
IE
Sharonov
V P
VP
Lyzlova
S N
SN
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D006997:Hypochlorous Acid / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D010539:Permeability; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D012834:Silver / Q000494:pharmacology*
D012834:Silver; D006997:Hypochlorous Acid; D002118:Calcium