Direct injection assay of angiotensin-converting enzyme by high-performance liquid chromatography using a shielded hydrophobic phase column.
A rapid and sensitive method for the determination of angiotensin-converting enzyme activity in serum and tissue extracts is described. The procedure is based on the high-performance liquid chromatographic separation of the synthetic substrate hippuryl-L-histidyl-L-leucine from the hydrolysis product hippuric acid. The separation is accomplished by direct injection of biological assay mixtures onto a shielded hydrophobic phase column with isocratic elution.
Doig
M T
MT
Department of Chemistry, College of Charleston, SC 29424.
Smiley
J W
JW
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006626:Hippurates / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D008168:Lung / Q000737:chemistry*; D009842:Oligopeptides / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000032:analysis* / Q000097:blood; D051381:Rats
D006626:Hippurates; D009842:Oligopeptides; C010980:hippuryl-histidyl-leucine; D007703:Peptidyl-Dipeptidase A; C030514:hippuric acid