Evaluation of contact sensitivity of rubber chemicals using the murine local lymph node assay.
The sensitizing abilities of 4 rubber additives, tetramethylthiuram disulfide (TMTD). 2-mercaptobenzothiazole (MBT), N-isopropyl-N'-phenyl-p-phenylenediamine (IPPD) and zinc diethyldithiocarbamate (ZDEC), were evaluated using the murine local lymph node assay. Exposure to IPPD induced a significant increase of lymph node cell proliferation in the draining lymph nodes even at a low concentration. Exposure to TMTD and MBT induced moderate proliferative responses, while ZDEC induced a weak proliferation even at the higher concentrations. The sensitizing potency of each chemical was described in terms of the concentration that increased lymph node cell proliferation by a factor of 2 over that in the vehicle-treated control group. The concentrations of IPPD, TMTD, MBT and ZDEC were 0.14%, 0.35%, 4.2% and > 10%, respectively.
National Institute of Hygienic Sciences, Tokyo, Japan.
Y
Y
Ikarashi
T
T
Tsuchiya
A
A
Nakamura
D000818:Animals; D052160:Benzothiazoles; D002455:Cell Division; D004050:Ditiocarb / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005260:Female; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010655:Phenylenediamines / Q000032:analysis / Q000276:immunology*; D012408:Rubber / Q000737:chemistry*; D012867:Skin / Q000166:cytology / Q000276:immunology*; D013844:Thiazoles / Q000032:analysis / Q000276:immunology*; D013893:Thiram / Q000032:analysis / Q000276:immunology*
D052160:Benzothiazoles; D010655:Phenylenediamines; D013844:Thiazoles; D013893:Thiram; C005563:captax; D012408:Rubber; D004050:Ditiocarb; C014627:N-isopropyl-N-phenyl-4-phenylenediamine