Identification of human type D retrovirus as a contaminant in a neuroblastoma cell line.
In this report we describe a type D virus isolated from a human neuroblastoma cell line (Paju). The viral RNA was isolated, partially molecularly cloned and sequenced. Our clones were shown to be identical to a human D-type retrovirus previously isolated from a human lymphoblastoid cell line. However, we obtained no evidence for the virus in earlier passage of the Paju cell line and therefore we must consider this isolate a laboratory contamination. contamination.
Department of Virology, University of Helsinki, Finland.
K
K
Asikainen
M
M
Vesanen
T
T
Kuittinen
A
A
Vaheri
D000293:Adolescent; D030121:Betaretrovirus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D009447:Neuroblastoma / Q000382:microbiology*; D012367:RNA, Viral / Q000302:isolation & purification; D014407:Tumor Cells, Cultured / Q000382:microbiology*
D012367:RNA, Viral