Quantification of tissue plasma volume in the rat by contrast-enhanced magnetic resonance imaging.
Magnetic resonance imaging enhanced with a macromolecular contrast medium (MMCM), albumin-Gd-DTPA, was used to estimate the plasma volume in vivo in the myocardium, lung, liver, and skeletal muscle of 10 normal rats. The plasma volumes of the same tissues in a parallel group of six rats were estimated in vitro by a conventional radioisotopic technique (111In-transferrin). Plasma volumes of myocardium, lung, liver, and skeletal muscle estimated by the MR technique (microliter plasma cc-1 of tissue) were 101, 109, 163, and 11.0, respectively, while plasma volumes measured by the 111In-transferrin radioisotope technique (mg plasma g-1 of tissue) were 78.6, 215, 143, and 11.2, respectively. Assuming a ratio of densities of aerated lung to blood of 0.45 and of other tissues to blood of 1.0, correlation between the methods was excellent (R2 = 0.99) indicating that MR imaging enhanced with MMCM permits reliable in vivo estimation of tissue plasma volume in the rat.
Contrast Media Laboratory, University of California, San Francisco, California.
R
R
Kuwatsuru
D M
DM
Shames
A
A
Mühler
J
J
Mintorovitch
V
V
Vexler
J S
JS
Mann
F
F
Cohn
D
D
Price
J
J
Huberty
R C
RC
Brasch
D000418:Albumins; D000818:Animals; D003287:Contrast Media; D003326:Coronary Circulation / Q000502:physiology; D005260:Female; D005682:Gadolinium; D019786:Gadolinium DTPA; D006321:Heart / Q000502:physiology; D007089:Image Enhancement / Q000379:methods; D007205:Indium Radioisotopes; D008099:Liver / Q000502:physiology; D008168:Lung / Q000502:physiology; D008279:Magnetic Resonance Imaging / Q000379:methods; D009132:Muscles / Q000502:physiology; D009942:Organometallic Compounds; D004369:Pentetic Acid; D010953:Plasma Volume / Q000502:physiology; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D014168:Transferrin; D014682:Vena Cava, Inferior / Q000502:physiology
D000418:Albumins; D003287:Contrast Media; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D014168:Transferrin; C058946:albumin-(gadolinium-DTPA); D004369:Pentetic Acid; D005682:Gadolinium; D019786:Gadolinium DTPA