Immunogold localisation of ubiquitin-protein conjugates in Sf9 insect cells. Implications for the biogenesis of lysosome-related organelles.
Immunogold electron microscopy with antibodies which primarily detect ubiquitin-protein conjugates shows conjugate-specific gold particles enriched severalfold in acid phosphatase-positive lysosomes and multivesicular bodies in insect Sf9 cells. The observations demonstrate that ubiquitinated proteins are associated with small acid phosphatase-containing primary lysosomes (transport vesicles) and indicate a pathway in which primary lysosomes fuse with multivesicular bodies to generate mature lysosome-related structures.
Department of General Zoology, Eötvös University, Budapest, Hungary.
P
P
Löw
F J
FJ
Doherty
M
M
Sass
J
J
Kovács
R J
RJ
Mayer
L
L
László
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D007150:Immunohistochemistry; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009036:Moths / Q000737:chemistry*; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis*; D014452:Ubiquitins / Q000032:analysis*
D011506:Proteins; D014452:Ubiquitins; D000135:Acid Phosphatase