Alginate immobilized mammalian neurons: a potential tool to isolate new neuronal ligands.
We developed improved immobilization conditions which permitted (i) to immobilize neuroblastoma cells (N18) in calcium-alginate gel beads, (ii) to test the function of ionic channels using patch-clamp electrophysiological techniques and (iii) to quantitatively analyze ligand interactions with voltage-dependent sodium channels in neurons inside the beads. These results qualify this immobilization technique for the isolation and/or purification of ligands specific for neuronal cells.
Boisseau
S
S
Laboratoire de Neurobiologie Cellulaire et Moléculaire CNRS, Gif sur Yvette, France.
Tamponnet
C
C
Poujeol
C
C
Lièvremont
M
M
Simonneau
M
M
D000464:Alginates; D000818:Animals; D002214:Capsules; D015816:Cell Adhesion Molecules, Neuronal / Q000302:isolation & purification; D002454:Cell Differentiation; D002478:Cells, Cultured; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007150:Immunohistochemistry; D008024:Ligands; D008564:Membrane Potentials; D008567:Membranes, Artificial; D051379:Mice; D008839:Microelectrodes; D009417:Nerve Tissue / Q000254:growth & development; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism*; D012530:Saxitoxin / Q000378:metabolism*; D015222:Sodium Channels / Q000502:physiology
D000464:Alginates; D002214:Capsules; D015816:Cell Adhesion Molecules, Neuronal; D006603:Hexuronic Acids; D008024:Ligands; D008567:Membranes, Artificial; D015222:Sodium Channels; D012530:Saxitoxin; D020723:Glucuronic Acid