Analysis of the rhesus cytomegalovirus immediate-early gene promoter.
A cytomegalovirus (CMV) isolate has been recovered from a rhesus macaque (Macaca mulatta) experimentally infected with simian immunodeficiency virus. The full-length immediate-early gene (IE) was cloned from a genomic library of this primary rhesus CMV isolate (RhCMV) using the African green monkey (AGM) CMV IE gene as probe. The promoter region and the first non-coding exon of the RhCMV IE gene have been sequenced. Nucleotide sequence comparisons from positions -733 to +625 (relative to the start site of transcription at +1) reveal 70 and 48% sequence homology with the AGM CMV IE and the human CMV (HCMV) IE genes, respectively. A region that includes the TATA box, the cis repression signal and the first exon in the RhCMV IE gene sequence is 91 and 76% homologous with the AGM CMV and HCMV IE genes, respectively. In permissive MRC-5 cells infected with RhCMV, synthesis of RNA from the IE gene peaks at 4-8 hr post infection. These studies provide a basis for elucidating molecular mechanisms regulating expression of the RhCMV IE gene and viral replication.
Department of Medical Pathology, University of California, Davis 95616.
D J
DJ
Alcendor
P A
PA
Barry
E
E
Pratt-Lowe
P A
PA
Luciw
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000209:etiology; D005260:Female; D006801:Humans; D017874:Immediate-Early Proteins; D008253:Macaca mulatta / Q000382:microbiology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications
D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus