[Changes in the amount of ATP in HeLa cells under the action of He-Ne laser radiation].
Amount of ATP in HeLa cells in various phases of growth was measured after He-Ne laser irradiation (100 J/m2, 10 W/m2, 10 s) by a bioluminescent luciferin-luciferase method. In cells of the exponential phase of growth, the amount of ATP (basal level 8 x 10(-16) mole/cell) starts to increase in 15 min after the irradiation with a maximum (170% above the basal level) at 20 min, and then decreases gradually to the basal level. The sensitivity of the cells to He-Ne laser radiation is lowest in lag-phase of growth, then increases to a plateau (approximately 185% above the control level) from 5th day of cultivation.
T I
TI
Karu
L V
LV
Piatibrat
G S
GS
Kalendo
N G
NG
Serebriakov
D000255:Adenosine Triphosphate / Q000032:analysis / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006367:HeLa Cells / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D007834:Lasers; D008163:Luminescent Measurements; D013997:Time Factors
D000255:Adenosine Triphosphate