Inhibitory effects of vasoactive intestinal peptide on superoxide anion formation by N-formyl-methionyl-leucyl-phenylalanine-activated inflammatory cells in vitro.
Effects of vasoactive intestinal peptide (VIP) on superoxide anion (O2-) formation by N-formyl-methionyl-leucyl-phenylalanine (fMLP)-activated inflammatory cells from healthy volunteers were investigated using 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo [1,2-a]pyrazin-3-one (MCLA) as a chemiluminescence probe. VIP inhibited the maximal light intensity of MCLA-dependent luminescence in a dose-dependent manner by the activated peripheral blood neutrophils, mononuclear cells and also by the human monoblast cell line U937, the capacity of which for O2- formation was induced by pretreatment with interferon-gamma. 3 x 10(-6) M VIP also inhibited O2- formation by the activated peripheral blood eosinophils and alveolar macrophages obtained by bronchoalveolar lavage.
Kurosawa
Motohiro
M
Ishizuka
Tamotsu
T
D004804:Eosinophils / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D008163:Luminescent Measurements; D016676:Macrophages, Alveolar / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D013481:Superoxides; D014660:Vasoactive Intestinal Peptide; D009240:N-Formylmethionine Leucyl-Phenylalanine