[Enkephalinase activity in the guinea pig model of asthma].
Enkephalinase exists in airway epithelial cells, smooth muscle, and submucosa near glands, and cleaves tachykinins to inactive metabolites, thereby reducing there effects. To study the role of enkephalinase in asthmatic response, we measured its activity in guinea pig model of asthma. When compared with the control values, the enkephalinase activity was reduced during in immediate asthmatic response (IAR) and late asthmatic response (LAR). Compared with the control values (100%), each value was 79.7%, 73.4% in the trachea and 74.3%, 55.7% in the lung respectively. Tracheal muscle preparation taken from the control, IAR, and LAR groups were made and mounted in oxygenated modified Krebs-Ringer solution. The response was monitored by isometric transducer. Concentration response curves to NKA with or without phosphoramidon were obtained. The contractile responses of the LAR groups were enhanced in potency and efficiency. Phosphoramidon potentiated the NKA induced contraction of control and the IAR groups but was less potent in enhancing the contractile response in the LAR group, showing less enkephalinase activity in the LAR. These results suggest that the enkephalinase plays an important role in LAR. In LAR, the enkephalinase activity may be inhibited and the responsiveness of the smooth muscle to some bronchoconstrictor, such as tachykinins, may be increased.
Division of Pulmonary Medicine, University of Tsukuba.
H
H
Ohse
A
A
Nomura
T
T
Endoh
Y
Y
Noguchi
M
M
Saotome
H
H
Ninomiya
A
A
Watanabe
J
J
To
K
K
Hashimoto
Y
Y
Uchida
D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000201:enzymology* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000201:enzymology; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000201:enzymology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D015260:Neprilysin / Q000378:metabolism* / Q000502:physiology; D015288:Neurokinin A / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects
D000485:Allergens; D015288:Neurokinin A; D015260:Neprilysin