Delayed-type hypersensitivity response to the human papillomavirus type 16 E7 protein in a mouse model.
To study the immune response to human papillomavirus type 16, a mouse model was developed using a mouse keratinocyte cell line expressing the E7 protein. This line was grafted onto syngeneic mice to form a differentiated epithelium, thus closely mimicking the natural infection. A delayed-type hypersensitivity response could be demonstrated after intradermal challenge with a vaccinia virus recombinant expressing the E7 protein. This response appeared to be specific for the E7 polypeptide and was mediated by CD4+ cells.
McLean
C S
CS
Department of Pathology, University of Cambridge, U.K.
Sterling
J S
JS
Mowat
J
J
Nash
A A
AA
Stanley
M A
MA
D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D004848:Epithelium / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D015603:Keratinocytes / Q000637:transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000276:immunology*; D050725:Papillomavirus E7 Proteins; D014162:Transfection; D014616:Vaccinia virus
D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16