Cloning of the YAP19 gene encoding a putative yeast homolog of AP19, the mammalian small chain of the clathrin-assembly proteins.
Mouse brain AP19 is the smallest polypeptide chain component of AP-1, the clathrin-assembly protein complex located at the Golgi complex. We isolated a gene, termed YAP19, potentially encoding a homolog of AP19 from the genomic DNA of the yeast Saccharomyces cerevisiae. The deduced amino acid sequence, 156 amino acids long, shows 53% identity with the mouse brain AP19 protein, but 37% with Yap17p, a yeast homolog of the mammalian AP17 in AP-2, another clathrin-assembly protein complex located at the plasma membrane. The identification of YAP19 supports the proposal that yeast cells also contain the Golgi-associated clathrin-assembly protein complex.
Nakai
M
M
Department of Chemistry, Faculty of Science, Nagoya University, Japan.
Takada
T
T
Endo
T
T
D033961:Adaptor Protein Complex 1; D033962:Adaptor Protein Complex 2; D034002:Adaptor Protein Complex sigma Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002966:Clathrin / Q000235:genetics*; D003001:Cloning, Molecular; D005800:Genes, Fungal; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
C499068:APS1 protein, S cerevisiae; D033961:Adaptor Protein Complex 1; D033962:Adaptor Protein Complex 2; D034002:Adaptor Protein Complex sigma Subunits; D033942:Adaptor Proteins, Vesicular Transport; C510066:Ap2s1 protein, mouse; D002966:Clathrin; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; C499071:adaptor protein complex 1, sigma 1 subunit