Identification of a linear neutralization site within the third variable region of the feline immunodeficiency virus envelope.
Synthetic peptides have been used to map linear B-cell epitopes of the third variable (V3) region of the feline immunodeficiency virus (FIV) external membrane glycoprotein gp120. The analysis of sera from naturally and experimentally FIV-infected cats by Pepscan and enzyme immunoassay with four partially overlapping peptides evidenced three antibody-binding domains, two of which mapped in the carboxyl-terminal half of V3. In particular, the V3.3 sequence (Gly-392-Phe-413) turned out to be important for in vitro neutralization of the virus in that the peptide inhibited the FIV-neutralizing activity of pooled immune cat sera, and on the other hand, cat sera raised against this peptide effectively neutralized FIV infectivity for Crandell feline kidney cells.
Department of Biomedicine, University of Pisa, Italy.
S
S
Lombardi
C
C
Garzelli
C
C
La Rosa
L
L
Zaccaro
S
S
Specter
G
G
Malvaldi
F
F
Tozzini
F
F
Esposito
M
M
Bendinelli
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D000917:Antibody Formation; D002415:Cats / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015686:Gene Products, env / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera; D016089:Immunodeficiency Virus, Feline / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D017433:Protein Structure, Secondary; D013997:Time Factors
D000914:Antibodies, Viral; D015686:Gene Products, env; D007106:Immune Sera; D010455:Peptides