Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel.
A cDNA encoding MDCK-type chloride channel was cloned from the rat kidney cDNA library using the homology screening approach. The deduced amino acid sequence of cloned cDNA has a 92.4% homology with the MDCK chloride channel, and electrophysiological analysis using Xenopus oocyte expression system showed the functional character of chloride channel. The rat kidney chloride channel, abbreviated RKCL, is not specific to the kidney, but widely expressed in various tissues. It is suggested that RKCL is important for most cells.
Department of Clinical Biology and Hormonal Regulation, Tohoku University School of Medicine, Sendai, Japan.
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D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018118:Chloride Channels; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004285:Dogs; D015870:Gene Expression; D007668:Kidney / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014981:Xenopus
D018118:Chloride Channels; D008565:Membrane Proteins; D004247:DNA