[The gel centrifugation test: comparison of antibody identification at 37 degrees C with antibody identification at room temperature].
The indirect antiglobulin test (antibody screening) (IAT) using the 'DiaMed-ID Micro Typing System' (ID system) was performed in two different ways: (1) incubation (Liss/Coombs version) at room temperature (RT) and (2) incubation (also Liss/Coombs version) at 37 degrees C. 106 antibody-containing sera were tested by IAT at RT and at 37 degrees C. The comparison of the results showed a high correlation between both methods: 49 of 106 antibodies were positive in the IAT at both RT and 37 degrees C, 51 antibodies were negative at both temperatures. Five antibodies were identified only in IAT performed at RT. 1/106 antibodies of the specificity anti-D (Rh prophylaxis) was not detected in the antibody screening at RT and in the conventional tube test. The antibody screening at RT using the ID System could be performed simultaneously with AB0 blood group typing without losing sensitivity and specificity. The modification of IAT using the ID System is more sensitive than the conventional tube test.
Transfusionszentrale, Johannes-Gutenberg-Universität, Mainz, BRD.
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Hitzler
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M
Ulrich
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C
Däumer
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Beeser
D000373:Agglutinins / Q000032:analysis; D001788:Blood Grouping and Crossmatching; D001831:Body Temperature; D002498:Centrifugation; D003298:Coombs Test; D003450:Cryoglobulins; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D007519:Isoantigens / Q000032:analysis*; D011237:Predictive Value of Tests; D012204:Rh-Hr Blood-Group System / Q000032:analysis
D000373:Agglutinins; D003450:Cryoglobulins; D007518:Isoantibodies; D007519:Isoantigens; D012204:Rh-Hr Blood-Group System; C029443:cold agglutinins