Cloning and sequence analysis of the alpha subunit of the cat flea sodium pump.
We have cloned a cDNA that encodes the sodium pump alpha-subunit of the cat flea Ctenocephalides felis. The deduced amino acid sequence of 1037 residues is 90% homologous with the Drosophila alpha-subunit sequence and shows 75% similarity to the sheep kidney alpha-subunit. One characteristic unique to the insect polypeptides is an extended sequence at the N-terminus. Northern blot analysis of C. felis RNA detects two alpha-subunit transcripts with lengths of 3.8 and 4.4 kb. Sodium pump alpha-subunit sequences were readily detected in homogenates of flea midguts and in cDNA synthesized from midgut RNA.
Paravax Inc., Mountain View, Calif.
S A
SA
Reeves
M K
MK
Yamanaka
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D004064:Digestive System / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D005423:Siphonaptera / Q000235:genetics* / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*
D015342:DNA Probes; D004247:DNA; D000254:Sodium-Potassium-Exchanging ATPase