Polyene antibiotics inhibit superoxide-producing NADPH oxidase in a polymorphonuclear cell-free system.
We studied the effect of polyene macrolide antibiotics on the NADPH-dependent superoxide production induced by arachidonic acid in a cell-free system consisting of the membrane and cytosolic fractions obtained from bovine polymorphonuclear leukocytes. Preincubation of the membrane fraction with polyenes before addition of the soluble components of the reaction mixture resulted in a dose-dependent inhibition of superoxide production.
Capuozzo
E
E
Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
Crifò
C
C
Salerno
C
C
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000494:pharmacology; D002417:Cattle; D002474:Cell-Free System / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D018942:Macrolides; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D019255:NADPH Oxidases; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D011090:Polyenes / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018942:Macrolides; D011090:Polyenes; D013481:Superoxides; D016718:Arachidonic Acid; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases