Molecular cloning of crustacean red pigment concentrating hormone precursor.
A cDNA encoding the precursor of the red pigment concentrating hormone (RPCH) of the shore crab, Carcinus maenas, was isolated and sequenced. The predicted preprohormone sequence consists of a putative 25 amino acid signal peptide, the eight amino acid RPCH sequence followed by a Gly as amide donor, a dibasic processing site and a 74 residue peptide of unknown function, referred to as RPCH-precursor-related peptide (RPRP). In in situ hybridisation experiments, the RPCH gene transcript could be localised in two clusters of neurons in the medulla terminalis of the eyestalk.
Linck
B
B
Institut für Zoophysiologie, University of Bonn, Germany.
Klein
J M
JM
Mangerich
S
S
Keller
R
R
Weidemann
W M
WM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000378:metabolism*; D003001:Cloning, Molecular; D015723:Gene Library; D017403:In Situ Hybridization; D007447:Invertebrate Hormones / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D009842:Oligopeptides / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*
D007447:Invertebrate Hormones; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D009842:Oligopeptides; D011498:Protein Precursors; D012333:RNA, Messenger; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid