Induced photolysis of rabbit red blood cells by several photosensitizers.
The photolysis of rabbit red blood cells induced by several photosensitizers has been studied. Membrane photohemolysis was found to be dependent on light dose (J/cm2) for hematoporphyrin (HP), methylene blue (MB) and toluidine blue (TB). No significant hemolysis was detected when mesotetra (4N-methylpyridyl) porphine (T4MPyP) plus light was used. Sodium azide inhibited, whereas deuterium oxide potentiated membrane lysis with HP, MB and TB. In the case of T4MPyP no differences were observed when using sodium azide or deuterium oxide.
Deparmento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
A
A
Juarranz
A
A
Villanueva
V
V
Díaz
L
L
Rodríguez-Borlado
C
C
Trigueros
M
M
Cañete
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000528:radiation effects; D004912:Erythrocytes / Q000187:drug effects* / Q000528:radiation effects*; D006415:Hematoporphyrins / Q000494:pharmacology; D006454:Hemoglobins / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis; D008027:Light; D008652:Mesoporphyrins / Q000494:pharmacology; D008751:Methylene Blue / Q000494:pharmacology; D010782:Photolysis; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology; D011817:Rabbits; D014048:Tolonium Chloride / Q000494:pharmacology
D006415:Hematoporphyrins; D006454:Hemoglobins; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D011166:Porphyrins; D014048:Tolonium Chloride; C021096:tetra(4-N-methylpyridyl)porphine; D008751:Methylene Blue