Monoclonal antibody recognizes different quinone moieties in enzymes.
We produced monoclonal antibodies against the coenzyme pyrrolequinoline quinone (PQQ). These antibodies were obtained by immunizing mice with PQQ conjugated to a chemically modified polypeptide in order to induce a strong immune response. Among the various antibodies obtained, one was found to bind (besides PQQ and 6-hydroxydopamine conjugated to carrier proteins) several different quinoenzymes, namely lentil seedling and bovine serum diamine oxidases and methylamine dehydrogenase. This antibody was able to inhibit the catalytic activity of these enzymes. Moreover, the monoclonal antibody recognized different proteins of lentil seeds on Western blots. Even the variable fragment of immunoglobulin heavy chains of this monoclonal antibody expressed in Escherichia coli is able to recognize the active site of different quinoenzymes.
Department of Experimental Medicine and Biochemical Sciences, Tor Vergata University of Rome, Italy.
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Marini
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Giardina
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GF
Fasciglione
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Finazzi-Agrò
D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000915:Antibody Affinity; D001665:Binding Sites; D002417:Cattle; D003067:Coenzymes / Q000276:immunology*; D007887:Fabaceae / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors / Q000276:immunology; D045542:PQQ Cofactor; D010946:Plants, Medicinal; D015363:Quinolones / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003067:Coenzymes; D015363:Quinolones; D045542:PQQ Cofactor; D006631:Amine Oxidase (Copper-Containing); C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors