Effect of ozone on metabolic activities of rat hepatocytes and mouse peritoneal macrophage.
Peritoneal macrophage from mice and isolated hepatocytes from rats were exposed to ozone. Ozone dosages were expressed as 0-5 nmol/10(6) cells. Measurements were made of viability, glucose transport, glutathione, glyceraldehyde-3-phosphate dehydrogenase, Mg-ATPase, Na/K-ATPase, and lipid synthesis. The most sensitive parameter was glyceraldehyde-3-phosphate dehydrogenase in the peritoneal macrophage. In hepatocytes both lipid synthesis and glyceraldehyde-3-phosphate dehydrogenase were sensitive to ozone. Effects on viability, glucose transport, Mg-ATPase, and Na/K-ATPase were small to negligible in both cell types.
Peters
R E
RE
University of California, Riverside.
Inman
C
C
Oberg
L
L
Mudd
J B
JB
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D010126:Ozone / Q000633:toxicity*; D010537:Peritoneum / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D008055:Lipids; D010126:Ozone; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose