Novel electrochemical device for the detection of cholesterol or glucose.
A thin-layer twin-electrode electrochemical cell in which the working and auxiliary electrodes are facing each other and which contains the appropriate enzyme and a mediator was used for the determination of cholesterol or glucose. A steady-state diffusion-controlled response was obtained for cholesterol whereas for glucose the response was limited by diffusion and kinetics. Simulation of the system showed that a steady-state response is obtained, in the absence of kinetic complications, after a time of 0.01 d2/Ds, where d is the distance between the two electrodes and D is the diffusion coefficient of the mediator. Linear plots of steady-state current versus concentration were obtained for cholesterol and glucose. The results were compared with those expected theoretically and a thin-layer capillary fill device is proposed.
Chemistry Department, Dublin Institute of Technology, Ireland.
J F
JF
Cassidy
C
C
Clinton
W
W
Breen
R
R
Foster
E
E
O'Donoghue
D002784:Cholesterol / Q000032:analysis*; D002789:Cholesterol Oxidase / Q000378:metabolism; D004058:Diffusion; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D007700:Kinetics; D009195:Peroxidase / Q000378:metabolism
D002784:Cholesterol; D005949:Glucose Oxidase; D002789:Cholesterol Oxidase; D009195:Peroxidase; D005947:Glucose