A human monoclonal hybridoma antibody (TrJ1) specific for HLA-DQ2.
TrJ1 is a cytotoxic human hybridoma mAb (IgM lambda). Its reaction pattern with a panel of 42 HLA-defined lymphoblastoid B-cell lines correlated precisely with expression of DQ2. By flow cytometry it was shown that the binding of TrJ1 to DQ2 was efficiently blocked by the murine anti-DQ2 mAb 358.4, indicating that the TrJ1 and 358.4 epitopes overlap. TrJ1 reacted much better with EBV-transformed B cells than with B cells freshly isolated from blood. TrJ1 seemed suitable for typing freshly isolated B cells provided the incubation with complement lasted for 115 min in Terasaki plates. One or more of the DQ2-specific polymorphic amino acids E46, F47, L52, L55, K71 or A74, situated on the alpha-helix of the DQ2 beta chain, are probably critical for the TrJ1 epitope.
Ge
J
J
Department of Immunology, University of Tromsø, School of Medicine, Norway.
Bartnes
K
K
Hannestad
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic; D005260:Female; D005434:Flow Cytometry; D006683:HLA-DQ Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006650:Histocompatibility Testing; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D007518:Isoantibodies / Q000276:immunology*; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D006683:HLA-DQ Antigens; C053186:HLA-DQ2 antigen; D007075:Immunoglobulin M; D007146:Immunoglobulin lambda-Chains; D007518:Isoantibodies