Analysis of the human dopamine beta-hydroxylase promoter: transcriptional induction by cyclic AMP.
We have analyzed some functional aspects of the promoter of the human dopamine beta-hydroxylase (DBH) gene. A fragment of 1,247 bp directly 5' to the transcriptional start was progressively shortened, placed in front of a reporter gene, and tested in a human neuroblastoma cell line expressing DBH (SK-N-SH-TFM) and in a monkey kidney cell line (CV-1). A remarkably short region (267 bp), directly upstream from the transcription start, was sufficient to confer activity and tissue-specific expression. Furthermore, the expression of the DBH gene was shown to be inducible by cyclic AMP in SK-N-SH-TFM cells. This effect was demonstrated to occur at the transcriptional level, as shown by run-on assays, and was due to the presence of a near-consensus cyclic AMP-responsive element located in the untranscribed 5' regulatory region of the gene.
Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, CNRS, Gif-sur-Yvette, France.
A
A
Lamouroux
L
L
Houhou
N F
NF
Biguet
G
G
Serck-Hanssen
B
B
Guibert
C
C
Icard-Liepkalns
J
J
Mallet
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D000242:Cyclic AMP / Q000494:pharmacology*; D004299:Dopamine beta-Hydroxylase / Q000235:genetics*; D005796:Genes / Q000187:drug effects; D000882:Haplorhini; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D015345:Oligonucleotide Probes; D000242:Cyclic AMP; D004299:Dopamine beta-Hydroxylase