[The role of cAMP in suppressing the maturation of follicle-enclosed oocytes in the common frog and the clawed toad after their treatment with actinomycin D].
During maturation of the follicle-enclosed Rana temporaria and Xenopus laevis oocytes stimulated by pituitary suspension or human chorionic gonadotropin, respectively, the level of cAMP (follicle+medium) increased. When maturation was stimulated by low concentrations of gonadotropic hormones (inducing; however, maturation in 100% or close to 100% cases), the cAMP level increased 1.5-3 fold. Both the increased concentration of gonadotropic hormones and pretreatment of the follicles with actinomycin D stimulated further increase in cAMP level. These treatments in combination led to a significant increase of cAMP and inhibition of oocyte maturation.
M N
MN
Skoblina
T
T
Matikainen
I
I
Huhtaniemi
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D000242:Cyclic AMP / Q000032:analysis / Q000502:physiology*; D003609:Dactinomycin / Q000494:pharmacology*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D010902:Pituitary Gland; D011896:Rana temporaria; D014020:Tissue Extracts / Q000494:pharmacology; D014982:Xenopus laevis
D006063:Chorionic Gonadotropin; D014020:Tissue Extracts; D003609:Dactinomycin; D000242:Cyclic AMP