Detection of bovine leukemia virus RNA in serum using the polymerase chain reaction.
A method was developed for detecting bovine leukemia virus (BLV) RNA in serum samples using a pair of primers from the BLV polymerase gene in the polymerase chain reaction (PCR). The PCR was able to detect 3800-7600 molecules of BLV RNA. At this level of sensitivity eleven pools of adult and one fetal bovine serum appeared free from BLV contamination.
Connaught Product Development Centre, Connaught Laboratories Limited, Willowdale, Ont., Canada.
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H
Poon
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Jimenez
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RM
Jacobs
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Song
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Jefferson
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics; D016583:Enzootic Bovine Leukosis / Q000517:prevention & control; D005069:Evaluation Studies as Topic; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary*; D012367:RNA, Viral / Q000097:blood / Q000235:genetics*; D012680:Sensitivity and Specificity; D014765:Viral Vaccines / Q000302:isolation & purification
D004279:DNA, Viral; D012367:RNA, Viral; D014765:Viral Vaccines