Production of interleukin 1 by rat pleural leucocytes in culture after intratracheal instillation of crocidolite asbestos.
This study was undertaken to investigate the production of interleukin 1 (IL-1) by pleural leucocytes in culture and to evaluate the influence of intratracheal instillation of crocidolite asbestos on this production. Normal pleural leucocytes spontaneously released IL-1 in culture and stimulation with lipopolysaccharide (LPS) dramatically increased production. Intratracheal instillation with crocidolite asbestos induced recruitment of pleural leucocytes in the longer term and changed IL-1 production by the leucocytes. Reduced production of IL-1 was found by one day after instillation of asbestos and this was correlated with the dose of asbestos. With increasing time after instillation, however, release of IL-1 by pleural leucocytes gradually recovered to normal until, one month after asbestos injection, the leucocytes produced augmented IL-1 in culture compared with control pleural leucocytes. Our data show that pleural leucocytes possess the potential to produce IL-1 in vitro and this capability is altered by intratracheal instillation of crocidolite asbestos. This may be relevant to development of pleural diseases associated with inhalation of asbestos.
Li
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XY
Department of Medicine (RIE), University of Edinburgh, City Hospital, UK.
Lamb
D
D
Donaldson
K
K
D000818:Animals; D001194:Asbestos / Q000494:pharmacology*; D017638:Asbestos, Crocidolite; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007375:Interleukin-1 / Q000096:biosynthesis*; D007962:Leukocytes / Q000378:metabolism*; D016131:Lymphocyte Subsets; D051381:Rats; D013997:Time Factors
D007375:Interleukin-1; D017638:Asbestos, Crocidolite; D001194:Asbestos