Comparative study of different immunoserological techniques for the detection of antibodies against transmissible gastroenteritis (TGE) coronavirus.
In order to carry out a sero-epidemiological survey of transmissible gastroenteritis (TGE) infection in a high-density pig-breeding area the Murcia region, Spain), the sensitivity and the specificity of 5 different techniques were compared. Twenty-four sera from non-infected and infected pigs were analyzed by the following tests: in vitro neutralization, indirect ELISA with concentrated virus, indirect ELISA with purified virus, immunocapture ELISA and VELCIA (virus-enzyme linked cell immunoassay). All sera from infected pigs were detected as positive only by seroneutralization, ELISA with purified virus and VELCIA (sensitivity 100%). False-positive sera were detected with several tests (ELISA with purified virus and concentrated virus, ELISA immunocapture with a specificity of 88, 88 and 76% respectively). Only VELCIA provided a specificity as high as that obtained with seroneutralization (100%).
Cubero
M J
MJ
Facultad de Veterinaria, Departamento de Patologia Animal, Murcia, Spain.
Bernard
S
S
Leon
L
L
Lantier
I
I
Contreras
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D002353:Carrier State / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D005761:Gastroenteritis, Transmissible, of Swine / Q000175:diagnosis / Q000276:immunology*; D009500:Neutralization Tests; D012680:Sensitivity and Specificity; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral