Serum free 5-methoxypsoralen fraction in health and psoriasis: relationship with human serum albumin concentration.
Human serum albumin is known to be the main carrier of 5-methoxypsoralen (5-MOP) in serum. As hypoalbuminaemia may occur in psoriasis with inflammatory syndrome, variability of the free 5-MOP fraction in serum can be expected. The free 5-MOP fraction was determined by equilibrium dialysis in serum samples obtained from 18 psoriatic patients and 18 control subjects. The median free 5-MOP fraction was not significantly different in the psoriatic group (fu = 4.75%) than in the control group (fu = 5%). However, there was a significantly larger variability of the free fraction in the psoriatic group (2.7 to 8.6%) than in the healthy group (3.2 to 6.8%) (p = 0.002). The binding index of 5-MOP (ratio of bound to free concentrations) was correlated with human serum albumin level (r = 0.784). This work confirms that the 5-MOP fraction in human serum is principally serum albumin dependent, as has been described with in vitro models. Free drug monitoring of 5-MOP is discussed.
Laboratoire de Biologie Cutanée, Centre Hospitalier Universitaire, Besançon, France.
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P
Muret
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P
Humbert
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S
Makki
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P
Bechtel
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Urien
J P
JP
Tillement
D000078223:5-Methoxypsoralen; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000378:metabolism; D008875:Middle Aged; D011701:PUVA Therapy; D011485:Protein Binding; D011565:Psoriasis / Q000097:blood / Q000188:drug therapy*; D012709:Serum Albumin / Q000032:analysis*
D012709:Serum Albumin; D000078223:5-Methoxypsoralen; D008730:Methoxsalen