Interfamilial and intrafamilial genomic diversity and molecular phylogeny of human T-cell lymphotropic virus type I from Papua New Guinea and the Solomon Islands.
To determine the interstrain genomic diversity and molecular phylogeny of the recently identified variants of human T-cell lymphotropic virus type I (HTLV-I) in Melanesia, we enzymatically amplified, then directly sequenced representative regions of the gag, pol, and env genes of HTLV-I strains from 10 members of four families, including one family from Papua New Guinea and three families from the Solomon Islands. When aligned and compared to a Japanese strain of HTLV-I (ATK), the Melanesian HTLV-I strains differed by 7.6 to 8.7% in the gag, 7.1 to 9.3% in the pol, and 7.3 to 8.2% in the env gene regions. Based on 931 nucleotides, the overall sequence divergence of the 10 Melanesian HTLV-I strains from HTLV-I ATK was 7.3 to 8.1% (68 to 75 base substitutions). The intrafamilial genetic heterogeneity among these virus strains was nil to 0.2%, while the interfamilial sequence variation between HTLV-I strains from the Solomon Islands and those from Papua New Guinea was 3.4 to 4.2%, and the genetic heterogeneity among virus strains from the three Solomon Islands families was 0.2 to 0.9%. Using the maximum parsimony and neighbor-joining methods, phylogenetic analysis indicated that the HTLV-I strains from Papua New Guinea and the Solomon Islands formed a monophyletic group and that the Melanesian and cosmopolitan strains of HTLV-I have evolved along two major geographically dependent lineages.
Nerurkar
V R
VR
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
Song
K J
KJ
Saitou
N
N
Melland
R R
RR
Yanagihara
R
R
D001483:Base Sequence; D005190:Family; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D015752:Genes, env / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral; D015490:HTLV-I Infections / Q000453:epidemiology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008542:Melanesia / Q000453:epidemiology; D008969:Molecular Sequence Data; D010219:Papua New Guinea / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid