Cysteamine gliopathy in situ: a cellular stress model for the biogenesis of astrocytic inclusions.
In cultured astroglia, cysteamine induces the accumulation of peroxidase-positive cytoplasmic inclusions in the context of a generalized cellular stress response. In the present study, systemic cysteamine administration over a 3 week period induced HSP27, 72, 90, and GRP94 (stress proteins) in astrocytes and significantly increased numbers of peroxidase-positive astrocytic inclusions in the various brain regions relative to controls. Similar patterns of HSP expression were also observed at 24 hours following cysteamine treatment indicating that cellular stress may be a very proximal event in the biogenesis of the astrocytic inclusions. The topography of glial peroxidase activity may provide a "map" of central nervous system regions particularly prone to oxidative stress during normal aging and under pathologic conditions.
Bloomfield Centre for Studies on Aging, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada.
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Schipper
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Mydlarski
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Wang
D000818:Animals; D001253:Astrocytes / Q000201:enzymology / Q000502:physiology*; D003543:Cysteamine / Q000009:adverse effects*; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009422:Nervous System Diseases / Q000139:chemically induced* / Q000473:pathology; D009457:Neuroglia / Q000187:drug effects* / Q000473:pathology*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000139:chemically induced / Q000473:pathology*
D006360:Heat-Shock Proteins; D003543:Cysteamine; D009195:Peroxidase