Development of a flow system for the microscopic observation of cultured nerve cells and its application to the neurite growth control of PC12h-R.
A flow system has been developed for the continual microscopic observation of cultured nerve cells. The undefined effects of coexisting cells could be efficiently reduced by the continuous flow of a medium. The present flow system was useful also for the experiments requiring medium exchanges. Its performance was demonstrated by the repeated actions of NGF or db-cAMP to PC12h-R cells. The neurite projection and its retraction were repeatedly observed by the supply and the removal of those factors.
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Y
Y
Kazuno
T
T
Homma
Y
Y
Nemoto
H
H
Matsuoka
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002452:Cell Count; D003470:Culture Media; D005434:Flow Cytometry / Q000295:instrumentation*; D009414:Nerve Growth Factors / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000166:cytology*; D016716:PC12 Cells / Q000166:cytology
D003470:Culture Media; D009414:Nerve Growth Factors; D003994:Bucladesine