Ultracytochemical study of ouabain-sensitive K(+)-dependent p-nitro-phenylphosphatase activity in stria vascularis of reserpinized guinea pigs.
In order to clarify the effect of reserpine on the Na(+)-K+ active transport in the stria vascularis we investigated the localization of ouabain-sensitive K(+)-dependent p-nitro-phenylphosphatase (K-NPPase) activity in the stria vascularis of reserpinized guinea pigs using the cerium-based method. The K-NPPase activity in marginal cells differed from cell to cell, and was almost completely absent in some cells. These results are consistent with the previous results and suggest that Na(+)-K+ active transport in the stria vascularis may be inhibited by reserpine administration.
Department of Otolaryngology, Hyogo College of Medicine, Nishinomiya, Japan.
N
N
Kanoh
T
T
Kumoi
T
T
Kobayashi
T
T
Okada
H
H
Seguchi
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003051:Cochlea / Q000737:chemistry* / Q000187:drug effects / Q000201:enzymology; D005260:Female; D006168:Guinea Pigs; D006651:Histocytochemistry; D008297:Male; D010042:Ouabain / Q000378:metabolism; D012110:Reserpine; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013316:Stria Vascularis / Q000737:chemistry / Q000166:cytology
D010042:Ouabain; D012110:Reserpine; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase