Two different promoters direct expression of two distinct forms of mRNAs of human platelet-activating factor receptor.
The human platelet-activating factor (PAF) receptor gene exists as a single copy on chromosome 1. We identified two 5'-noncoding exons, each of which has distinct transcriptional initiation sites. These exons are alternatively spliced to a common splice acceptor site on a third exon that contains the total open reading frame to yield two different species of functional mRNA (Transcript 1 and 2). Transcript 1 has consensus sequences for transcription factor NF-kappa B and Sp-1, and the Initiator (Inr) sequence homologous to the murine terminal deoxynucleotidyltransferase gene. Transcript 2 also contains consensus sequences for transcription factor AP-1, AP-2, and Sp-1. Transcripts 1 and 2 were both detected in heart, lung, spleen, and kidney, whereas only Transcript 1 was found in peripheral leukocytes, a differentiated human eosinophilic cell line (EoL-1 cells), and brain. Existence of distinct promoters was thus suggested to play a role in the regulatory control of PAF receptor gene expression in different human tissues and cells.
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
H
H
Mutoh
H
H
Bito
M
M
Minami
M
M
Nakamura
Z
Z
Honda
T
T
Izumi
R
R
Nakata
Y
Y
Kurachi
A
A
Terano
T
T
Shimizu
D017398:Alternative Splicing; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010980:Platelet Membrane Glycoproteins; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled; D014158:Transcription, Genetic
D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor