Typing of human papillomavirus DNAs by restriction endonuclease mapping of the PCR products.
The polymerase chain reaction (PCR) for the diagnosis of human papillomavirus (HPV) infections, and in particular for the study of cervical HPV-associated lesions, is used widely. We identified a novel set of universal primers that are able to amplify a fragment spanning the E1 open reading frame (ORF) from different mucosotropic HPV types. A restriction endonuclease digestion of the amplified products is suggested for accurate typing. In particular, AluI digestion of the amplified fragments yields a distinctive fragment pattern for each 'high-risk' (16, 18, 31 and 33) HPV sequence, thus distinguishing them from 'low-risk' (6b and 11) HPV sequences.
Immunobiological Research Institute Siena, Italy.
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Contorni
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Leoncini
D001483:Base Sequence; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000145:classification / Q000235:genetics*; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured / Q000382:microbiology; D014412:Tumor Virus Infections / Q000175:diagnosis; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000382:microbiology; D002575:Uterine Cervicitis / Q000175:diagnosis; D014773:Virology / Q000379:methods*
D015344:DNA Probes, HPV; D004279:DNA, Viral