Coactivation of metabotropic and NMDA receptors is required for LTP induction.
Induction of long-term potentiation (LTP) of synaptic transmission in the CA1 region of the hippocampus is dependent on N-methyl-D-aspartate (NMDA) receptor activation. However, NMDA receptor stimulation alone is not adequate to trigger LTP. Results from the present study indicate that, in addition to NMDA receptors, there is a requirement for co-stimulation of a postsynaptic metabotropic glutamate receptor (mGluR). The mGluR involved in eliciting LTP is not antagonized by L-2-amino-3-phosphonopropionic acid (L-AP3, 500 microM), nor is it coupled to phosphoinositide metabolism. We demonstrate that a novel mGluR, which is stimulated by trans-ACPD and is linked to increase in adenosine 3',5'-monophosphate (cAMP), is physiologically relevant to the induction of LTP.
Musgrave
M A
MA
Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, Canada.
Ballyk
B A
BA
Goh
J W
JW
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000187:drug effects / Q000502:physiology; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects; D013873:Thionucleotides / Q000494:pharmacology
D010716:Phosphatidylinositols; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D013873:Thionucleotides; C000211:2-amino-3-phosphonopropionic acid; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D015124:8-Bromo Cyclic Adenosine Monophosphate; C016957:adenosine-3',5'-cyclic phosphorothioate; D016202:N-Methylaspartate; D002217:Carbachol; D000242:Cyclic AMP; D000409:Alanine