Gene 5 of the IDIR agent (group B rotavirus) encodes a protein equivalent to NS34 of group A rotavirus.
Gene 5 of the IDIR strain of group B rotavirus was cloned, sequenced, and expressed in RBC lysates in order to identify the coding assignment. The complete nucleic acid sequence of IDIR gene 5 included 1306 bases and encoded a single long open reading frame of 390 amino acids with a predicted molecular weight of 44.8 kDa. Comparison of the predicted amino acid sequence indicated substantial identity with the NS34 protein of group A rotaviruses (GAR). Like GAR NS34, the product deduced from IDIR agent gene 5 was predicted to exhibit a high degree of alpha helix secondary structure and a relatively low pl (4.6). In vitro translation of gene 5 resulted in synthesis of a protein which was specifically immunoprecipitated by convalescent antibody obtained from infant rats following infection with the IDIR agent, confirming that this product was of viral origin. Under non-reducing conditions, products as large as 200 kDa were also noted in SDS-PAGE. Formation of oligomers by GAR NS34 has previously been observed, indicating that this feature is probably a general characteristic among rotavirus NS34 and may relate to the functional role of the protein in viral replication.
Eiden
J J
JJ
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D017433:Protein Structure, Secondary; D051381:Rats; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D017361:Viral Nonstructural Proteins / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
C082740:NSP3 protein, Rotavirus; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C071648:gene 9 protein, Rotavirus B