The influence of high salt intake on intestinal Na,K-ATPase in Wistar and Dahl rats.
The Na,K-ATPase of intestinal mucosa was compared in Wistar (W), salt-sensitive (DS) and salt-resistant (DR) Dahl rats fed a low-salt (LS) and high-salt (HS) diet. ATPase activity and the kinetics of its activation by Na+ were determined in three intestinal segments (jejunum, ileum, distal colon). There were demonstrated only small differences in the affinity for Na+ among the strains studied but we found a considerable profile of Na,K-ATPase activity along the intestine in all strains; the activity was higher in jejunum and lower in ileum and distal colon. Chronic salt loading decreased the affinity of Na,K-ATPase for Na+ but had no effect on Vmax. The changes in salt intake were accompanied by different response of plasma aldosterone in particular strains. According to the stimulation of aldosterone level by LS diet the sensitivity of the strains was DR > W > DS. HS diet suppressed aldosterone level to similar values in all strains. The data indicate that the kinetics of intestinal Na,K-ATPase and its response to HS intake is independent of the genotype of the rats.
Pácha
J
J
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Pohlová
I
I
D000450:Aldosterone / Q000097:blood / Q000502:physiology; D000818:Animals; D003106:Colon / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D007082:Ileum / Q000201:enzymology*; D007413:Intestinal Mucosa / Q000201:enzymology; D007583:Jejunum / Q000201:enzymology*; D007700:Kinetics; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012964:Sodium / Q000032:analysis / Q000493:pharmacokinetics / Q000494:pharmacology; D012965:Sodium Chloride / Q000032:analysis / Q000493:pharmacokinetics / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000502:physiology
D012965:Sodium Chloride; D000450:Aldosterone; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase