Decreased adenylate energy charge and superoxide anion release in polymorphonuclear leukocytes in septic patients after hepatectomy.
To clarify the process of multiple organ failure after hepatectomy, the adenylate energy charge (EC) and superoxide anion (O2-) release of polymorphonuclear leukocytes (PMN) were measured 19 times on 11 septic patients after curative hepatic resection in relation to arterial ketone body ratio (AKBR), which reflects hepatic mitochondrial redox potential. PMN EC was measured by labeling the adenine nucleotide pool of PMN with radioactive adenine. The data of the septic patients (n = 19) was divided into two groups according to the hepatic energy status: AKBR above 0.7 (septic state A; n = 8) and AKBR below 0.7 for at least 3 successive days (septic state B; n = 11). Thirteen nonseptic patients undergoing hepatic resection were applied to the control group, and all of them showed AKBRs higher than 0.7. The PMN EC of septic patients was significantly lower than that of nonseptic patients [0.80 +/- 0.01 (SEM) vs. 0.88 +/- 0.02, P < 0.01]. In septic patients, moreover, the PMN EC of septic state A was significantly higher than septic state B (0.85 +/- 0.01 vs. 0.77 +/- 0.03, P < 0.05). Superoxide anion release of septic patients was significantly lower than that of nonseptic patients (19.0 +/- 2.1 nmol/5 x 10(5) cells/15 min vs. 29.3 +/- 1.7 nmol/5 x 10(5) cells/15 min, P < 0.01). Superoxide anion release of septic state A was significantly greater than that of septic state B (26.0 +/- 2.8 nmol/5 x 10(5) cells/15 min vs. 15.0 +/- 1.9 nmol/5 x 10(5) cells/15 min, P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Egawa
H
H
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Higashiyama
H
H
Iwata
S
S
Mori
K
K
Sasada
M
M
Kagawa
R
R
Wada
Y
Y
Shimahara
Y
Y
Ozawa
K
K
D000227:Adenine Nucleotides / Q000097:blood; D000368:Aged; D001424:Bacterial Infections / Q000150:complications / Q000209:etiology* / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D006498:Hepatectomy / Q000009:adverse effects*; D006801:Humans; D007657:Ketone Bodies / Q000097:blood; D008297:Male; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000378:metabolism; D009102:Multiple Organ Failure / Q000209:etiology; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000097:blood
D000227:Adenine Nucleotides; D007657:Ketone Bodies; D013481:Superoxides