Nucleotide sequence analysis of the CDNA for rat DNA topoisomerase II.
CDNA clones encoding the rat DNA topoisomerase II were isolated from rat testis CDNA library using a DNA probe synthesized by two sequential nested PCRs. The nucleotide sequence of the entire coding region and its deduced 1526 amino acid sequence showed that 80% nucleotides and 89% amino acids were identical with human HeLa DNA topoisomerase II gene (hTOP2). Approximately 1100 amino acids at the N-terminus shows 96.5% sequence identity, but C-terminus has only 65% homology. Rat DNA topoisomerase II gene (rTOP2) contains three functional domains responsible for ATPase activity, break-reunion activity, and complex stability and DNA binding activity like other eukaryotic TOP2. It also contains two putative nuclear targeting sequences and a leucine zipper motif and has highly charged species specific sequences at the C-terminus.
Department of Molecular Biology, Seoul National University, Republic of Korea.
S H
SH
Park
J H
JH
Yoon
Y D
YD
Kwon
S D
SD
Park
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D015342:DNA Probes; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology*
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D004247:DNA; D004250:DNA Topoisomerases, Type II