Inhibitory effect of urate on oxidative damage induced by adriamycin-Fe3+ in the presence of H2O2.
Urate strongly inhibited DNA damage induced by adriamycin (AD)-Fe3+ in the presence of H2O2. Deoxyribose (DOR) was degraded during the interaction of Ad-Fe3+ with H2O2, and urate strongly inhibited the DOR degradation. However, hydroxyl radical (HO.) scavengers did not always prevent the DOR degradation. ESR signal of 5,5-dimethyl-1-pyroline N-oxide-hydroxyl radical adduct was detected during the interaction of Ad-Fe3+ with H2O2. Mannitol and DNA strongly diminished the ESR signal but urate only poorly did. These results suggested that inhibitory effect of urate on the H2O2-dependent AD-Fe(3+)-induced DNA damage was not always due to trap only HO.. Lipid peroxidation of erythrocyte membrane was induced by AD-Fe3+ alone and H2O2 slightly inhibited the peroxidation reaction. Urate could not inhibit the Ad-Fe(3+)-induced lipid peroxidation. These results indicate that oxygen radical species which causes the lipid peroxidation does not involve in the H2O2-dependent Ad-Fe(3+)-induced DNA damage.
Miura
T
T
Department of Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Otaru, Japan.
Muraoka
S
S
Ogiso
T
T
D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage; D003855:Deoxyribose / Q000378:metabolism; D004317:Doxorubicin / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008353:Mannitol / Q000494:pharmacology; D009942:Organometallic Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity; D010084:Oxidation-Reduction; D010957:Plasmids; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D014527:Uric Acid / Q000494:pharmacology*
D000975:Antioxidants; D009942:Organometallic Compounds; C041415:doxorubicin-iron complex; D014527:Uric Acid; D008353:Mannitol; D003855:Deoxyribose; D004317:Doxorubicin; D006861:Hydrogen Peroxide; D011493:Protein Kinase C