Prolonged biochemical and morphological stability of encapsulated liver cells--a new method.
In this work a new type of polyelectrolyte complex capsules is introduced as an artificial housing for liver cells. Male Wistar rat hepatocytes were encapsulated using cellulose sulphate and polydimethyldialyllammonium chloride as polyelectrolytes. Amino acid metabolism rate and urea synthesis of the cells increased over the investigation period in contrast to the decrease observed in control monolayer cultures. The encapsulated cells were morphologically characterized. The described procedure represents a sufficient method for the cultivation of living cells in mechanically stable semipermeable microcapsules.
Department of Internal Medicine, University Rostock, FRG.
J
J
Stange
S
S
Mitzner
H
H
Dautzenberg
W
W
Ramlow
M
M
Knippel
M
M
Steiner
B
B
Ernst
R
R
Schmidt
H
H
Klinkmann
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002214:Capsules; D002478:Cells, Cultured; D002482:Cellulose / Q000031:analogs & derivatives; D004339:Drug Compounding / Q000379:methods; D008099:Liver / Q000166:cytology*; D008297:Male; D008567:Membranes, Artificial; D010539:Permeability; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000378:metabolism
D000596:Amino Acids; D002214:Capsules; D008567:Membranes, Artificial; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D014508:Urea; D002482:Cellulose; C020254:cellulose sulfate