[Study of the self-association of deoxytetraribonucleoside triphosphate molecules d(ApGpCpT) in aqueous solution by one-dimensional and two- dimensional (1)H NMR spectroscopy].
Equilibrium behaviour of self-complimentary deoxytetranucleotides d(ApGpCpT) in D2O solution has been studied by the method of proton magnetic resonance spectroscopy (500 MHz). Complete assignments of the d(ApGpCpT) protons were obtained from both two-dimensional homonuclear COSY- and NOESY-experiments. Concentration and temperature dependences of tetranucleotide proton chemical shifts have been investigated. The equilibrium association constants and thermodynamical parameters of self-association of the molecules have been calculated using theoretical models and regression equations based on experimental results.
A N
AN
Veselkov
D
D
Dévis
L N
LN
Dymant
Kh
Kh
Parkes
D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011522:Protons; D013696:Temperature; D014867:Water / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D011522:Protons; D014867:Water