Transpositional activity of IS986 in Mycobacterium smegmatis.
The insertion sequence IS986 (also known as IS6110) has been widely used for typing Mycobacterium tuberculosis isolates, due to the extensive multiple polymorphism shown using this probe. Although this polymorphism is presumed to be due to the mobility of IS986, transposition of this element has not previously been demonstrated in the laboratory. Using artificial composite transposons constructed in a vector unable to replicate in mycobacteria, we have succeeded in demonstrating the mobility of IS986 in M. smegmatis, apparently through cointegrate formation and transposition. The apparently random nature of IS986 insertions in M. tuberculosis is advantageous for transposon mutagenesis, although its efficient use will require more effective delivery systems.
Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, UK.
N G
NG
Fomukong
J W
JW
Dale
D001483:Base Sequence; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D004252:DNA Mutational Analysis / Q000379:methods; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004274:DNA, Recombinant / Q000096:biosynthesis; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D009161:Mycobacterium / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014162:Transfection / Q000379:methods*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004274:DNA, Recombinant