Genus-specific detection of salmonellae using the polymerase chain reaction (PCR).
Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.
Cohen
N D
ND
Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Neibergs
H L
HL
McGruder
E D
ED
Whitford
H W
HW
Behle
R W
RW
Ray
P M
PM
Hargis
B M
BM
D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification