[Cleavage of O-glycosyl bonds in glycopeptides].
The possibility of cleavage of the alpha-bond between mannose (or galactose) and serine (or threonine) in the presence of alpha-mannosidase and alpha-galactosidase has been studied. Using model compounds simulating the O-glycosyl bond in glycoproteins, several glycopeptides have been synthesized: N-tertbutyloxycarbonyl-O-alpha-mannopyranosyl-seryl-glycine methylamide (alpha-Man-Ser-Gly), tertbutyl-oxycarbonyl-O-alpha-mannopyranosyl-threonyl- glycine methylamide (alpha-Man-Thr-Gly), N-tertbutyloxy-carbonyl-O-alpha-galactopyranosyl-seryl-glycine methylamide (alpha-Gal-Ser-Gly) as well as N-tertbutyloxy-carbonyl-O-beta-mannopyranosyl-seryl-glycine methylamide (beta-Man-Ser-Gly). The cleavage has been shown to occur in glucoamylase after proteolytic degradation.
Ibatullin
F M
FM
Neustroev
K N
KN
Golubev
A M
AM
Firsov
L M
LM
D000595:Amino Acid Sequence; D006020:Glycopeptides / Q000378:metabolism*; D006868:Hydrolysis; D008361:Mannosidases / Q000378:metabolism; D008969:Molecular Sequence Data; D000519:alpha-Galactosidase / Q000378:metabolism; D043323:alpha-Mannosidase
D006020:Glycopeptides; D008361:Mannosidases; D000519:alpha-Galactosidase; D043323:alpha-Mannosidase