Antibodies to vesicular stomatitis virus proteins in patients with systemic lupus erythematosus and in normal subjects.
OBJECTIVE
To determine the presence of antibodies that bind vesicular stomatitis virus (VSV) proteins in systemic lupus erythematosus (SLE) patient sera, as well as to examine any relationship between antibodies binding the viral nucleocapsid (N) protein and anti-Ro autoantibodies.


METHODS
Eighty SLE patient sera, 80 normal control sera, 47 rheumatic disease patient control sera, and 9 sera from Panamanian subjects (6 of whom were infected with VSV) were evaluated by immunoblot of purified VSV proteins.


RESULTS
Findings on immunoblots of the Panamanian subjects' sera were consistent with histories of endemic exposure to VSV, as determined by virus neutralization assays. Significantly different binding to viral proteins was seen in the group of 80 SLE patients compared with the normal control group and with the rheumatic disease control group, by a variety of measures. For example, most of the reactivity in the SLE patient sera included binding to the internal viral matrix (M) and N proteins, while the reactivity in the normal control sera was mostly against the surface viral glycoprotein (G) protein alone or the N protein alone. Within this SLE patient cohort, associations were found between binding to N protein and anti-Ro precipitins and between binding to M protein and anti-nuclear RNP autoantibody precipitins.


CONCLUSIONS
The data are consistent with the original specific hypotheses of a relationship between 60-kd Ro autoantigenicity and VSV, as well as with the suggestion that a rhabdovirus may be important in SLE. They do not, however, make possible any conclusions concerning the role of rhabdoviruses in the development of SLE or of anti-Ro autoimmunity.
Hardgrave
K L
KL
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Neas
B R
BR
Scofield
R H
RH
Harley
J B
JB
D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D001323:Autoantibodies / Q000032:analysis; D015153:Blotting, Western; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D010176:Panama / Q000453:epidemiology; D010750:Phosphoproteins / Q000378:metabolism; D011485:Protein Binding; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D014777:Virus Diseases / Q000097:blood* / Q000453:epidemiology
D000914:Antibodies, Viral; D001323:Autoantibodies; D006023:Glycoproteins; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins