Fasciclin I and II have distinct roles in the development of grasshopper pioneer neurons.
We have used a new technique, micro-CALI (chromophore-assisted laser inactivation), to investigate the function of the neural cell adhesion molecules fasciclin I and II in the development of the grasshopper Ti1 neurons. Micro-CALI of fasciclin I results in defasciculation of the Ti1 axons similar to that achieved using large scale CALI (Jay and Keshishian, 1990). The initial point of axon separation corresponds to the site of laser irradiation, and defasciculation always continues distal to this point. Micro-CALI of fasciclin II prevents the initiation of Ti1 axon outgrowth but has no effect on fasciculation. This effect is restricted to a 3 hr interval between cytokinesis and growth cone emergence.
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
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Diamond
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Mallavarapu
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Schnipper
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Booth
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Park
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O'Connor
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Jay
D000818:Animals; D001369:Axons / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D015816:Cell Adhesion Molecules, Neuronal / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology; D006110:Grasshoppers / Q000196:embryology; D007834:Lasers; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure
D015816:Cell Adhesion Molecules, Neuronal; C051641:fasciclin I; C051642:fasciclin II