Establishment of an astrocyte progenitor cell line: induction of glial fibrillary acidic protein and fibronectin by transforming growth factor-beta 1.
An immortalized clonal cell line (AP-16) has been established from glial cultures obtained from neonatal mouse cerebra by multipassages under serum-free conditions. Immunofluorescent experiments showed that AP-16 cells expressed a marker for glial progenitors (A2B5) and did not express markers for oligodendrocytes (galactocerebroside) or mature astrocytes (glial fibrillary acidic protein: GFAP). Treatment with transforming growth factor-beta 1 (TGF-beta 1) or fetal calf serum (FCS) for 2 days induced AP-16 cells to differentiate into A2B5-negative, GFAP-positive, phenotypically mature astrocytes. AP-16 cells depended on epidermal growth factor for survival, and their growth was inhibited by FCS. These results indicate that AP-16 cells retained the properties of astrocyte progenitors. An enzyme-linked immunosorbent assay showed that AP-16 cells synthesized fibronectin and laminin, and that the expression of fibronectin was increased by TGF-beta 1. AP-16 cells should be useful for studying the roles of TGF-beta 1 in the differentiation of astrocyte progenitors.
Yoshida
T
T
Institute for Fermentation, Osaka, Japan.
Takeuchi
M
M
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015415:Biomarkers; D001790:Blood Physiological Phenomena; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis* / Q000235:genetics; D006133:Growth Substances / Q000494:pharmacology; D007797:Laminin / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D015415:Biomarkers; D016895:Culture Media, Serum-Free; D005353:Fibronectins; D005904:Glial Fibrillary Acidic Protein; D006133:Growth Substances; D007797:Laminin; D009419:Nerve Tissue Proteins; D016212:Transforming Growth Factor beta; D003994:Bucladesine