Subunit identification and reconstitution of the N-type Ca2+ channel complex purified from brain.
Calcium channels play an important role in regulating various neuronal processes, including synaptic transmission and cellular plasticity. The N-type calcium channels, which are sensitive to omega-conotoxin, are involved in the control of transmitter release from neurons. A functional N-type calcium channel complex was purified from rabbit brain. The channel consists of a 230-kilodalton subunit (alpha 1B) that is tightly associated with a 160-kilodalton subunit (alpha 2 delta), a 57-kilodalton subunit (beta 3), and a 95-kilodalton glycoprotein subunit. The complex formed a functional calcium channel with the same pharmacological properties and conductance as those of the native omega-conotoxin-sensitive calcium channel in neurons.
Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City 52242.
D R
DR
Witcher
M
M
De Waard
J
J
Sakamoto
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C
Franzini-Armstrong
M
M
Pragnell
S D
SD
Kahl
K P
KP
Campbell
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000378:metabolism; D015220:Calcium Channels / Q000737:chemistry / Q000502:physiology*; D007120:Immunochemistry; D010446:Peptide Fragments / Q000502:physiology; D010455:Peptides / Q000378:metabolism; D011817:Rabbits; D011869:Radioligand Assay; D017981:Receptors, Neurotransmitter / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D020866:omega-Conotoxin GVIA
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D010446:Peptide Fragments; D010455:Peptides; D017981:Receptors, Neurotransmitter; C053157:omega-conotoxin receptor; D020866:omega-Conotoxin GVIA