Respective role of the 42- and 51-kDa components of the Bacillus sphaericus toxin overexpressed in Bacillus thuringiensis.
The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as inclusion bodies which were purified. For the first time, the 42-kDa protein inclusions alone were found to be toxic to Culex pipiens larvae (LC50 at 48 h 300 ng ml-1); in contrast, the 51-kDa protein inclusions were not. Moreover, a synergistic effect between these two components was observed.
Nicolas
L
L
Unité des Bactéries Entomopathogènes, Institut Pasteur, Paris, France.
Nielsen-Leroux
C
C
Charles
J F
JF
Delécluse
A
A
D000818:Animals; D001407:Bacillus / Q000235:genetics*; D001413:Bacillus thuringiensis / Q000235:genetics / Q000648:ultrastructure; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000633:toxicity*; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects; D015870:Gene Expression; D005798:Genes, Bacterial; D002479:Inclusion Bodies / Q000648:ultrastructure; D007814:Larva / Q000187:drug effects; D008854:Microscopy, Electron; D008970:Molecular Weight; D010957:Plasmids; D015183:Restriction Mapping
D001427:Bacterial Toxins; C046134:binB protein, Bacillus sphaericus