A cluster of strong topoisomerase II cleavage sites is located near an integrated human immunodeficiency virus.
The Human Immunodeficiency Virus (HIV) integrates into host cellular DNA as a double strand DNA molecule. Here a previously studied HIV isolate was examined for binding and cleavage by topoisomerase II in vitro within the 5' LTR region and human flanking DNA. A cluster of strong binding and cleavage sites in the human sequences was located approximately 850 bp upstream from the integration site. This region maps to a locus consisting of a complex repeating element, and alternating purine/pyrimidine sequences. Topoisomerase II binding and cleavage sites were also located within the HIV 5' LTR, in particular a site overlying the DNA sequence coding for TAR, another inverted repeat element in the DNA.
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill 27599.
M T
MT
Howard
J D
JD
Griffith
D001483:Base Sequence; D002874:Chromosome Mapping; D004250:DNA Topoisomerases, Type II / Q000378:metabolism* / Q000648:ultrastructure; D004274:DNA, Recombinant / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D015894:Genome, Human; D006678:HIV / Q000235:genetics*; D016325:HIV Long Terminal Repeat / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D016662:Virus Integration
D004274:DNA, Recombinant; D004279:DNA, Viral; D004250:DNA Topoisomerases, Type II