Functional analysis of apf1 mutation causing defective amino acid transport in Saccharomyces cerevisiae.
Mutation in the Apf1 locus causes a pleiotropic effect of H(+)-driven active amino acid transport in baker's yeast Saccharomyces cerevisiae. The uptake of other, presumably H(+)-driven, substances, e.g. of purine and pyrimidine bases, maltose and phosphate ions, is not significantly influenced by this mutation. The apf1 mutation decreases not only the initial rates of amino acid uptake but also the accumulation ratios of amino acids taken up but has virtually no effect on the membrane potential or on the delta pH which constitute the thermodynamically relevant source of energy for their transport. Similarly, no changes in intracellular ATP content, in ATP-hydrolyzing and H(+)-extruding H(+)-ATPase activities, in the efflux of intracellularly accumulated amino acids, or in rates of endogenous respiration, were observed in the apf1 mutant phenotype. Hence, all these data are in accordance with the experiments showing that the Apf1 protein, an integral protein of the endoplasmic reticulum, is required exclusively for efficient processing and translocation of transport proteins specific for amino acids from the endoplasmic reticulum to their final destination, the plasma membrane.
Horák
J
J
Department of Membrane Transport, Czech Academy of Sciences, Prague.
Kotyk
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D001693:Biological Transport, Active; D004721:Endoplasmic Reticulum / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008320:Maltose / Q000378:metabolism; D008564:Membrane Potentials; D009154:Mutation; D010710:Phosphates / Q000378:metabolism; D011522:Protons; D011687:Purines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D000596:Amino Acids; D005656:Fungal Proteins; D010710:Phosphates; D011522:Protons; D011687:Purines; D011743:Pyrimidines; D008320:Maltose; D000255:Adenosine Triphosphate