Polyamines modulate the function of transfected glutamate receptor mGluR1a.
Metabotropic glutamate receptor mGluR1a was expressed in human embryonic kidney 293 cells. 1S,3R-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD) stimulated dose-dependently, phosphoinositide (PI) hydrolysis in transfected, but not in non-transfected cells. The polyamine spermine did not affect PI hydrolysis in the absence of 1S,3R-ACPD even at a concentration of 1 mM, but it potentiated the stimulatory action of 1S,3R-ACPD at 10 microM. The modulatory action of spermine was mimicked by spermidine but not by the short polyamine putrescine.
Manev
R M
RM
Fidia Research Laboratories, Abano Terme (PD), Italy.
Gabellini
N
N
Manev
H
H
D015317:Biogenic Polyamines / Q000502:physiology*; D002460:Cell Line; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007668:Kidney / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D011247:Pregnancy; D011700:Putrescine / Q000494:pharmacology; D017470:Receptors, Glutamate / Q000235:genetics / Q000502:physiology*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D014162:Transfection / Q000502:physiology*
D015317:Biogenic Polyamines; D010716:Phosphatidylinositols; D017470:Receptors, Glutamate; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D013096:Spermine; D013095:Spermidine; D011700:Putrescine