Vectors lambda 200g and lambda 200c: two useful derivatives of lambda 2001.
We describe the construction and uses of two new phage lambda replacement vectors. Both are modifications of lambda 2001 which allows selection of cloned inserts by the Spi- phenotype, following the replacement of a gam+ filler fragment. Vector lambda 200g has a second gam gene with an amber mutation in its right arm which enables the selection of clones with inserts in the normal way in Su0 host bacteria and the maintenance of their genetic stability by subsequent growth in Su+ strains. Vector lambda 200c is designed for complementation studies. It contains a cat gene and the attP site of lambda. Recombinants can be inserted into the bacterial chromosome or into an episome with the aid of appropriate helpers.
MRC Molecular Genetics Unit, University of Cambridge, United Kingdom.
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Koh
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Barnett
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Brenner
D001287:Attachment Sites, Microbiological; D010582:Bacteriophage lambda / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D016153:Genes, Suppressor; D005814:Genes, Viral; D005816:Genetic Complementation Test / Q000379:methods; D005822:Genetic Vectors; D009154:Mutation; D013489:Suppression, Genetic
D004274:DNA, Recombinant; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes