The octamer binding protein Oct-2 inhibits transactivation of the herpes simplex virus immediate-early genes by the virion protein Vmw65.
Transactivation by a complex of the cellular transcription factor Oct-1 and the virion protein Vmw65 is necessary for the high-level activity of the HSV immediate-early promoters during lytic infection. We show that this trans-activation can be inhibited by two forms of the Oct-2 transcription factor which are expressed at high levels in neuronal cells as well as by the isolated DNA binding, POU domain of Oct-2. The inhibition of Oct-1-Vmw65 DNA binding by these neuronal forms of Oct-2 is likely to play a critical role in the nonpermissivity of neuronal cells for the HSV lytic cycle and therefore in the establishment of latent infections.
Division of Molecular Pathology, University College London Medical School, United Kingdom.
K A
KA
Lillycrop
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Estridge
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Latchman
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins; D005091:Exons / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics*; D017874:Immediate-Early Proteins; D008969:Molecular Sequence Data; D050813:Octamer Transcription Factor-2; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D018139:Simplexvirus / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014771:Virion / Q000235:genetics
D004268:DNA-Binding Proteins; D017405:Herpes Simplex Virus Protein Vmw65; C050678:IE1 protein, Human herpesvirus 1; D017874:Immediate-Early Proteins; D050813:Octamer Transcription Factor-2; C058435:POU2F2 protein, human; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D015500:Chloramphenicol O-Acetyltransferase