Immunodiffusion and immunohistochemical investigations on the reactivity of oxide ceramic middle-ear implants.
Oxide ceramic materials (partial and total ossicular replacement prostheses) have been implants of preference for the reconstruction of the ossicular chain because of their excellent biocompatibility. The reaction on the surface of the implants takes place at three biodynamic levels according to the model of Stern's bilayer. We investigated the adsorption of proteins, which is determined by the cellular reaction and degradation to the surface using radial immunodiffusion and immunohistochemical methods. First, ceramic implants of aluminum oxide, hydroxyapatite, glass ceramic and zirconium oxide have individual actual (i.e. biological) surfaces. With a perthometer and the contact-free laser Focodyn method we determined each actual (i.e. biological) surface of the various ceramic implants mentioned above. Using radial immunodiffusion, the adsorption of albumin, glycoprotein, plasminogen, fibronectin, IgA, IgG and IgM shows characteristic rates of adsorption to the respective ceramic surfaces in correlating to the actual surface. A cross-check with fluorescent antibodies confirmed the protein adsorption. The individual surface adsorption of the proteins remains characteristic and is the basis for the recording of cellular reactions after implantation.
Otorhinolaryngology Division, Mannheim Hospital, Faculty of Clinical Medicine, University of Heidelberg, FRG.
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A
Schadel
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G
Thun
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L
Stork
R
R
Metzler
D000327:Adsorption; D000537:Aluminum Oxide; D001672:Biocompatible Materials; D001798:Blood Proteins / Q000493:pharmacokinetics*; D002516:Ceramics; D017886:Durapatite; D005898:Glass; D006801:Humans; D006882:Hydroxyapatites; D005779:Immunodiffusion; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D009998:Ossicular Prosthesis; D016062:Porosity; D015040:Zirconium
D001672:Biocompatible Materials; D001798:Blood Proteins; D006882:Hydroxyapatites; D017886:Durapatite; D015040:Zirconium; D000537:Aluminum Oxide; C028541:zirconium oxide