Epstein-Barr virus (EBV) genomes and EBV-encoded latent membrane protein (LMP) in pulmonary lymphomas occurring in nonimmunocompromised patients.
Recently, in situ hybridization (ISH) techniques have shown that Epstein-Barr virus (EBV) could be detected in tumor cells of most angiocentric T-cell non-Hodgkin's lymphomas (NHL). These studies included only a few cases of T-NHL of the lung and pulmonary B-NHL and have not been investigated. Furthermore, the expression of the EBV-encoded latent membrane protein (LMP), which is known for its oncogenic properties, has not been reported. Twelve pulmonary NHL (six angiocentric T-NHL and six B-NHL) arising in nonimmunocompromised patients were examined for the presence of EBV-EBER mRNAs and LMP with ISH and immunohistochemistry, respectively. Four cases of pulmonary lymphomas arising in immunocompromised patients were also included in the study for comparison (one T-NHL in a patient under immunosuppressive treatment and three B-NHL in AIDS patients). EBV-RNA and LMP were detected in tumor cells in two of six nonimmunocompromised angiocentric T-NHL and in the four immunocompromised NHL. The six nonimmunocompromised B-NHL were EBV negative. These results suggest that EBV is associated with some angiocentric pulmonary T-NHL arising in patients without overt immunodeficiency whereas it is absent in such patients with B-NHL. The presence of the transforming EBV-encoded LMP in tumor cells suggests that EBV may be involved in the pathogenesis of some pulmonary T-NHL.
Département de Pathologie, Hôpital Henri Mondor, Créteil, France.
J C
JC
Sabourin
P
P
Kanavaros
J
J
Briere
M C
MC
Lescs
T
T
Petrella
E S
ES
Zafrani
P
P
Gaulard
D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007121:Immunocompetence; D016867:Immunocompromised Host; D016130:Immunophenotyping; D017403:In Situ Hybridization; D008175:Lung Neoplasms / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D014412:Tumor Virus Infections / Q000276:immunology* / Q000473:pathology; D014763:Viral Matrix Proteins / Q000032:analysis*
D000956:Antigens, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D012367:RNA, Viral; D014763:Viral Matrix Proteins