High-performance liquid chromatography coupled to high-field proton nuclear magnetic resonance spectroscopy: application to the urinary metabolites of ibuprofen.
The use of coupled reversed-phase high-performance liquid chromatography and high-field proton nuclear magnetic resonance spectroscopy (HPLC-NMR) for the detection and identification of the urinary metabolites of ibuprofen is described. Urine was obtained from a healthy human volunteer following a normal therapeutic dose of 400 mg of ibuprofen. Analysis was performed on both a freeze-dried urine concentrate and partially purified extracts obtained by solid-phase extraction onto C-18 bonded silica gel. Both continuous and stop-flow methods were used to obtain spectra enabling the major urinary metabolites of ibuprofen to be detected and identified.
Bruker Analytische Messtechnik, Rheinstetten, Germany.
M
M
Spraul
M
M
Hofmann
P
P
Dvortsak
J K
JK
Nicholson
I D
ID
Wilson
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007052:Ibuprofen / Q000378:metabolism / Q000652:urine*; D009682:Magnetic Resonance Spectroscopy; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry
D058428:Silica Gel; D012822:Silicon Dioxide; D007052:Ibuprofen