Two cis-acting signals control ribosomal frameshift between human T-cell leukemia virus type II gag and pro genes.
The open reading frame of the human T-cell leukemia virus type II pro gene is arranged at a -1 position relative to the gag gene. Synthesis of the Gag-Pro fusion polyprotein is facilitated by ribosomal frameshift into the reading frame of the pro gene. Cloning of a synthetic 41-bp oligonucleotide corresponding to the gag-pro junction within a heterologous gene (nef of human immunodeficiency virus type I) and mutation analysis revealed that two cis-acting signals, an adenosine residue stretch and a dyad symmetry sequence, flanking the UAA termination codon, are required for efficient ribosomal frameshifting between gag and pro. The stability of the stem-loop structure is crucial for frameshifting.
Department of Molecular Genetics, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
H
H
Falk
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N
Mador
R
R
Udi
A
A
Panet
A
A
Honigman
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism*; D016368:Frameshift Mutation; D005814:Genes, Viral; D015750:Genes, gag; D015754:Genes, nef; D015497:HIV-1 / Q000235:genetics; D015367:Human T-lymphotropic virus 2 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism*; D013728:Terminator Regions, Genetic; D014764:Viral Proteins / Q000096:biosynthesis
D004279:DNA, Viral; D014764:Viral Proteins