Modification of a high-performance liquid chromatographic method for assay of homocysteine in human plasma.
A modification of a previously published method for analysis of total homocysteine in human serum is presented. The modification was implemented to allow use of a different derivatizing agent (i.e., 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonamide) which reacts much faster than the original derivatizing reagent and at a lower temperature. Shorter reaction time and lower temperature lead to less destruction of some biological thiols. In order to retain an isocratic mobile phase with the new derivatizing agent, a different concentration of acetonitrile was found that affords a 7-8 min retention time.
Department of Nutrition Sciences, University of Alabama, Birmingham 35294.
P E
PE
Cornwell
S L
SL
Morgan
W H
WH
Vaughn
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D005464:Fluorobenzenes / Q000737:chemistry; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D005464:Fluorobenzenes; D006710:Homocysteine; C037802:7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate