Desensitization of neurokinin A receptors expressed by B82 fibroblasts.
After chronic exposure to neurokinin A, a time-dependent and recoverable desensitization of inositol monophosphate accumulation occurred in B82 fibroblasts transfected with cDNAs encoding for the bovine stomach NK2 receptor. Desensitized cells also showed decreased Ca2+ mobilization. While NK2 receptor antagonists had no effect on inositol monophosphate accumulation, substance P and senktide both produced a small degree of desensitization.
Department of Pharmacology, University of Arizona, College of Medicine, Tucson 85724.
A K
AK
Henderson
W R
WR
Roeske
T L
TL
Smith
H I
HI
Yamamura
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA; D005347:Fibroblasts; D007295:Inositol Phosphates / Q000378:metabolism; D015288:Neurokinin A / Q000494:pharmacology*; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D007295:Inositol Phosphates; D018041:Receptors, Neurokinin-2; D017981:Receptors, Neurotransmitter; D015288:Neurokinin A; D004247:DNA; D002118:Calcium