Establishment and characterization of B-like lymphoblastoid cell lines by long-term culture of primary explants from human myasthenic thymus.
Using a simple method of long-term culture, it was possible to obtain B-like lymphoblastoid cell lines (LyCLs) from myasthenic thymuses. Successful cultures were carried out from 14 out of 15 hyperplastic thymuses and in 1 out of 3 myasthenic thymoma, whereas none of the 8 control thymuses, nor the 2 Myasthenia gravis-associated normally involuted thymuses, nor the Myasthenia gravis-associated lymphoma gave rise to LyCL. All the LyCLs secreted immunoglobulins (Ig), either IgG or IgM. None of these Ig reacted with acetylcholine receptor or with other antigens known to be often involved in autoimmune diseases. EBV antigens were found in all the LyCLs as well as in the corresponding donors at the time of thymectomy. HLA characterization of some LyCLs and the corresponding donors showed that class II MHC antigens were expressed normally or with mild differences. However, 86% of the LyCL tested did not express class I MHC antigens.
Université Louis Pasteur (ULP), Département d'Immunologie, Immunopharmacologie et Pathologie (Equipe de Biologie Cellulaire et de Neurologie Clinique), Illkirch, France.
J T
JT
Vilquin
S
S
Braun
P
P
Labouret
C
C
Tranchant
D
D
Hartmann
Y
Y
Lombard
J
J
Goetz
A
A
Urlacher
J M
JM
Wihlm
J M
JM
Warter
D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D002460:Cell Line; D003584:Cytological Techniques; D004848:Epithelium / Q000473:pathology; D006680:HLA Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D009157:Myasthenia Gravis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D010641:Phenotype; D011950:Receptors, Cholinergic / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology* / Q000382:microbiology / Q000473:pathology
D006680:HLA Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011950:Receptors, Cholinergic