Comparison of immunological reactivity to two major antigens of homologous and AD169 human cytomegalovirus strains during primary infection in renal transplant patients.
The IgG and IgM immunoblotting patterns for the major HCMV antigens p65 and p55/52 were studied in 5 renal transplant recipients during primary HCMV infections. Sequential sera from the 5 patients were tested in parallel with standardized antigens derived from the reference strain AD169 and from the patient'own isolates. The immunoblotting patterns differed from patient to patient as well as between strain AD169 and the patient's homologous isolates. The differences consisted in a better response to the antigens derived from the reference strain AD169, suggesting a delay of the IgM/IgG switch and of the affinity maturation of IgG antibodies to the homologous p65 and p55/52 antigens. The results suggest caution in the interpretation of immunoblotting data derived from laboratory strains such as AD169.
Department of Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
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Rossier
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Miller
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Dal Monte
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Boccuni
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Landini
D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis; D000915:Antibody Affinity; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology* / Q000302:isolation & purification; D002648:Child; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000209:etiology / Q000276:immunology*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000276:immunology* / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; C080634:human cytomegalovirus antigen p55-52