Translation and fates of the gag protein of 1731, a Drosophila melanogaster retrotransposon.
An entire copy of 1731, a Drosophila melanogaster retrotransposon, was tagged by fusing in frame its putative gag gene with the reporter LacZ sequence. The high transfection efficiency of Drosophila virilis cells added to the absence of 1731 in their genome allowed, by combining histochemical staining and immunological detections, the demonstration of the translation of the 1731 gag gene. The gag protein is gathered in virus-like particles. Its occurrence in nuclei is consistent with a nuclear localization signal. The expression of the sense construction was inhibited by cotransfections with its antisense homologue.
Groupe de Génétique Cellulaire et Moléculaire, URA-CNRS 1135, Paris, France.
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Kim
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Coulondre
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Champion
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Lacoste
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Best-Belpomme
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Maisonhaute
D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics); D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D004251:DNA Transposable Elements / Q000235:genetics*; D004331:Drosophila melanogaster; D015870:Gene Expression; D015683:Gene Products, gag / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014771:Virion / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D016375:Antisense Elements (Genetics); D004251:DNA Transposable Elements; D015683:Gene Products, gag; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase