Evidence for biosynthesis of plasmenylcholine from plasmenylethanolamine in HL-60 cells.
Both [3H]plasmenylethanolamine and [3H]plasmenylcholine were produced from substrates of [3H]alk-1-enylglycerol and [3H]alk-1-enyllysoglycerophosphoethanolamine by intact HL-60 cells. Molecular species analysis of the [3H]plasmenylcholine and [3H]plasmenylethanolamine formed indicated the major portion of plasmenylcholine originates from plasmenylethanolamine by a series of reactions catalyzed by phospholipase A2, lysophospholipase D, acyltransferase, phosphohydrolase, and cholinephosphotransferase. However, a significant but much smaller portion of the plasmenylcholine appeared to be synthesized from plasmenylethanolamine via a direct base-exchange or a coupled phospholipase C/cholinephosphotransferase reaction.
Medical Sciences Division, Oak Ridge Institute for Science and Education, TN 37831-0117.
M L
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Blank
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Fitzgerald
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Lee
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Snyder
D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D006801:Humans; D007938:Leukemia; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010955:Plasmalogens / Q000096:biosynthesis* / Q000378:metabolism*; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism; D000099189:Lysophospholipase D
D002798:Diacylglycerol Cholinephosphotransferase; D010741:Phospholipases A; D054467:Phospholipases A2; D010727:Phosphoric Diester Hydrolases; D010955:Plasmalogens; D014316:Tritium; D010738:Type C Phospholipases; D000099189:Lysophospholipase D; C032459:choline plasmalogens; C020791:phosphatidal ethanolamines