[Molecular and ultrastructural study on the relationship between human papillomavirus and female genital condyloma in China].
From the results of DNA hybridization, it has been shown that there is a close relationship between HPV 16 and human uterine cervical carcinoma in China, and also that the viral DNA is integrated into the cancer cell genome. Genital condyloma revealed a high positive hybridization rate with HPV 11 DNA, and the viral DNA presented as an episome apart from the cell genome. Under the electron microscope, the inter and perichromatin granules as well as nuclear bodies were observed in all biopsies with positive hybridization, but no HPV particle was found in cancer cells, besides these two kinds of chromatin granules. Human papillomavirus nucleocapsid particles were observed in the nuclei of some genital condyloma cells. Electron-dense matrix aggregates of different sizes were seen in the perinuclear cytoplasm and in the nuclei. This kind of structure may be related to the process of HPV maturity, but its nature remains to be studied.
Institute of Basic Medical Sciences, Beijing.
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Si
D002294:Carcinoma, Squamous Cell / Q000382:microbiology / Q000648:ultrastructure; D003218:Condylomata Acuminata / Q000382:microbiology* / Q000648:ultrastructure; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D002583:Uterine Cervical Neoplasms / Q000382:microbiology* / Q000648:ultrastructure; D014846:Vulvar Neoplasms / Q000382:microbiology*
D004279:DNA, Viral