Immunocytochemical localization of rat substance P receptor in the striatum.
A trp E fusion protein containing a C-terminal portion of the rat substance P receptor (SPR) was expressed in bacteria and used to produce an antibody. The antibody specifically reacted with SPR expressed in a mammalian cell line and rat striatum. Light and electron microscope analyses of the rat striatum revealed intense SPR-like immunoreactivity in neuronal somata and dendrites. These immunoreactive neurons constituted approximately 3% of the total population of striatal neurons; they were putative interneurons of large and medium-sized aspiny type.
Department of Morphological Brain Science, Faculty of Medicine, Kyoto University, Japan.
R
R
Shigemoto
Y
Y
Nakaya
S
S
Nomura
R
R
Ogawa-Meguro
H
H
Ohishi
T
T
Kaneko
S
S
Nakanishi
N
N
Mizuno
D000818:Animals; D003342:Corpus Striatum / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D003712:Dendrites / Q000276:immunology / Q000648:ultrastructure; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D013045:Species Specificity
D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter