Phagocytic function of monocyte-derived macrophages is not affected by human immunodeficiency virus type 1 infection.
The immunopathogenesis of human immunodeficiency virus (HIV) infection is characterized by the failure to control opportunistic infections. Here, the direct effect of HIV on macrophage phagocytic function was studied. HIV-1-infected monocyte-derived macrophages expressed as many Fc gamma and complement receptors as did control macrophages. The function of these receptors was not affected by HIV-1 infection since binding and internalization of opsonized Escherichia coli and Staphylococcus aureus were not impaired. Production of reactive oxygen species induced by stimulation of the HIV-1-infected macrophages with opsonized E. coli, zymosan, or PMA was intact. HIV-1-infected macrophages killed opsonized E. coli and Candida albicans as effectively as did control macrophages. These results, therefore, do not support the hypothesis that HIV-1 infection of macrophages causes phagocytic dysfunction and suggest that HIV-induced abnormalities outside the mononuclear phagocyte system may lead to the inability to control opportunistic pathogens.
Nottet
H S
HS
Eijkman-Winkler Laboratory for Medical Microbiology, University of Utrecht, Netherlands.
de Graaf
L
L
de Vos
N M
NM
Bakker
L J
LJ
van Strijp
J A
JA
Visser
M R
MR
Verhoef
J
J
D000954:Antigens, Surface / Q000096:biosynthesis; D002176:Candida albicans / Q000276:immunology; D002478:Cells, Cultured; D004926:Escherichia coli / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000502:physiology*; D007700:Kinetics; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D009000:Monocytes / Q000166:cytology / Q000382:microbiology; D010587:Phagocytosis; D016133:Polymerase Chain Reaction; D013481:Superoxides / Q000378:metabolism
D000954:Antigens, Surface; D013481:Superoxides