Growth-promoting action of adenosine-containing dinucleotide on neuroblastoma cells: detection of adenosine-cytidine dinucleotide (ApCp) in neurofibroma (NF1) extracts.
Neurofibroma type 1 tissue was investigated for the presence of growth-promoting activity on human neuroblastoma cells. The activity was isolated by gel filtration and reversed-phase column chromatographs from neurofibroma type 1 extracts. An adenosine-containing dinucleotide (adenylyl(3'-5')cytidine-3'-phosphate) was identified as one of the major components of the activities by its enzymatic fragmentation and liquid chromatography/mass spectrometry. Synthetic adenosine-containing dinucleotide derivatives such as cytidyl(3'-5')adenosine, cytidyl(2'-5')adenosine, adenylyl(3'-5')cytidine, and adenylyl(2'-5')cytidine showed a similar action. Cytidyl(3'-5')adenosine, cytidyl(2'-5')adenosine, and adenylyl(2'-5')cytidine, which are able to release a free adenosine through enzymatic hydrolysis, in particular elicited a strong activity corresponding to that of adenosine with the highest action. These results suggest that neuroblastoma cells are able to use adenosine-containing dinucleotides as well as mononucleotides for their survival and proliferation.
Hotta
T
T
Department of Bioregulation Research, Nagoya City University Medical School, Japan.
Asai
K
K
Takeda
N
N
Yoshizumi
H
H
Tatematsu
A
A
Nakanishi
K
K
Eksioglu
Y Z
YZ
Isobe
I
I
Kato
T
T
D000241:Adenosine / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D002853:Chromatography, Liquid; D015226:Dinucleoside Phosphates / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D013058:Mass Spectrometry; D009447:Neuroblastoma / Q000473:pathology*; D009455:Neurofibroma / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry* / Q000494:pharmacology*; D014020:Tissue Extracts / Q000737:chemistry*
D015226:Dinucleoside Phosphates; D009711:Nucleotides; D014020:Tissue Extracts; C083343:adenylyl(3'-5')cytidine-3'-phosphate; D000241:Adenosine