Maturation, capacitation, and metabolism of goat spermatozoa using magnetic resonance methods.
The ability of intact cells to reduce spin labels has been utilized to characterize the activity of spermatozoa of goat. The kinetics of reduction of TEMPO has been found to be sensitive to the quantity, quality and state of epididymal maturation of the spermatozoa. Presence of alcohol caused activation and Gossypol acetic acid left sperm activity unaltered. Electron spin resonance (ESR) and 31P Nuclear magnetic resonance (NMR) indicate that the period of in vitro capacitation requires optimization. 31P NMR spectra indicate a good correlation with the progressive maturation of the cells.
Chemical Physics Group, Tata Institute of Fundamental Research, Bombay, India.
S
S
Srivastava
R
R
Kaur
S R
SR
Bawa
R S
RS
Phadke
H K
HK
Bains
S
S
Sehgal
G
G
Govil
D000818:Animals; D003080:Cold Temperature; D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006041:Goats; D006072:Gossypol / Q000031:analogs & derivatives / Q000494:pharmacology; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; D006072:Gossypol; C028178:gossypol acetic acid; C003959:TEMPO