Comparison of detection speed and yield in BACTEC NR-860 and Roche BCB blood culture systems.
Detection speed and yield were compared between BACTEC NR-860 and Roche BCB blood culture systems. From a total of 1,550 paired blood cultures inoculated with the same volume of blood, 161 (10.4%) grew 180 organisms from 140 adult patients. Sixty-six percent of the isolates were clinically significant. The BACTEC system detected more clinically significant isolates than the BCB system, 91% vs 76% (p < 0.01), especially Enterobacteriaceae. In 39 paired blood cultures, 42 significant organisms were detected at different times in the two systems. The vast majority (93%) were detected first (on average 1 day faster) by the BACTEC system (p < 0.05), especially Escherichia coli and streptococci. Generally, detection times were shorter in the BACTEC system. Three fourths (76%) of all significant organisms were detected within the first incubation day as compared to less than half (42%) in the BCB system (p < 0.001). Generally, the agitated aerobic resin medium BACTEC 26 Plus had the fastest detection and highest yield in our study. The contamination rate, below 2% in both systems, was acceptable. The BACTEC NR-860 system with resin-containing media proved to be a reliable, sensitive and fast blood culture system, which deserves further investigation.
Department of Clinical Microbiology, Frederiksberg Hospital, Denmark.
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D000328:Adult; D001322:Autoanalysis / Q000379:methods; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D006801:Humans