Lithium gamma-linolenate-induced cytotoxicity against cells chronically infected with HIV-1.
Lithium gamma-linolenate (Li-GLA), was evaluated for its activity in selectively killing H9 cells chronically infected with HIV-1RF. After 4 days incubation with Li-GLA approximately 90% of the H9RF cells were non-viable compared to 20% of uninfected H9 cells. The efficacy of the Li-GLA, in preferentially killing HIV infected cells also correlates with lipid peroxidation, as measured by the intracellular thiobarbituric acid-reactive material content. The addition of an antioxidant (vitamin E) to the culture medium reduced the toxicity of Li-GLA. These data indicate that this selective killing effect of cells chronically infected with HIV may be due to the enhanced extent of lipid peroxidation of the added Li-GLA.
Department of Virology, Medical College of St. Bartholomew's Hospital, West Smithfield, London, UK.
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Kinchington
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Randall
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Winther
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Horrobin
D000998:Antiviral Agents / Q000494:pharmacology*; D002459:Cell Fusion; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015497:HIV-1 / Q000187:drug effects* / Q000302:isolation & purification; D008042:Linolenic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation; D014810:Vitamin E / Q000494:pharmacology; D017965:gamma-Linolenic Acid
D000998:Antiviral Agents; D008042:Linolenic Acids; D014810:Vitamin E; D017965:gamma-Linolenic Acid