Proton spin-lattice relaxation time as liver transplantation graft viability parameter.
In this study proton NMR T1 of stored rat liver grafts was investigated and correlated with other viability parameters. Five different storage solutions were used and T1 as well as overall tissue water content, bile flow, and energy charge were determined after various periods of cold storage (1 to 48 hr). A good correlation between T1 and tissue water content was detected, suggesting the possible use of this relaxation time parameter as liver graft viability parameter in a combined protocol of rapid viability assays for human liver transplantation surgery.
Holzmüller
P
P
Arbeitsgruppe-NMR, Universität Wien, Austria.
Moser
E
E
Reckendorfer
H
H
Burgmann
H
H
Sperlich
M
M
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001639:Bicarbonates; D001646:Bile / Q000378:metabolism; D001834:Body Water / Q000737:chemistry; D002122:Calcium Chloride; D002314:Cardioplegic Solutions; D004734:Energy Metabolism; D005947:Glucose; D005978:Glutathione; D006085:Graft Survival / Q000502:physiology*; D006859:Hydrogen; D006982:Hypertonic Solutions; D007328:Insulin; D008099:Liver / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology*; D008274:Magnesium; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008353:Mannitol; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011189:Potassium Chloride; D011343:Procaine; D011522:Protons; D011887:Raffinose; D051381:Rats; D012965:Sodium Chloride; D012996:Solutions; D014022:Tissue Survival / Q000502:physiology*; D014325:Tromethamine
D001639:Bicarbonates; C039984:Bretschneider cardioplegic solution; D002314:Cardioplegic Solutions; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; C074097:Krebs-Henseleit solution; D019188:Organ Preservation Solutions; D011522:Protons; D012996:Solutions; C041711:St. Thomas' Hospital cardioplegic solution; C055206:University of Wisconsin-lactobionate solution; D014325:Tromethamine; D008353:Mannitol; D012965:Sodium Chloride; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D006859:Hydrogen; D005978:Glutathione; D008274:Magnesium; D005947:Glucose; D000241:Adenosine; D002122:Calcium Chloride; D011887:Raffinose