A method for rapidly assembling complex plasmid constructs without propagation of intermediates in E. coli.
Department of Cellular and Molecular Biology, Merck Research Laboratories, Rahway, NJ 07065.
L O
LO
Palladino
G E
GE
Mark
G F
GF
Hollis
D001709:Biotechnology; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005821:Genetic Techniques; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes