Analysis of leukotrienes, prostaglandins, thromboxane B2 and their metabolites by capillary isotachophoresis.
An analytical capillary isotachophoretic method for the analysis of the eicosanoids leukotriene E4, leukotriene B4, prostaglandins E1 and E2, 6-keto-prostaglandin F1 alpha, thromboxane B2, and their metabolites of omega- and/or beta-oxidation is described. The method is based on anionic separation and detection by UV absorbance (254 nm) and conductivity and allows simultaneous analysis of the primary compounds and their corresponding major urinary metabolites. The method was applicable to the qualitative and quantitative analysis of prostaglandin E1 in a drug preparation.
Department of Clinical Pharmacology, Hannover Medical School, Germany.
D
D
Tsikas
J
J
Fauler
S
S
Bracht
G
G
Brunner
J C
JC
Frölich
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis / Q000652:urine; D000527:Alprostadil / Q000032:analysis / Q000652:urine; D002197:Capillary Action; D015232:Dinoprostone / Q000032:analysis / Q000652:urine; D004586:Electrophoresis / Q000379:methods*; D007975:Leukotriene B4 / Q000032:analysis; D017999:Leukotriene E4; D015289:Leukotrienes / Q000032:analysis* / Q000652:urine; D010084:Oxidation-Reduction; D011453:Prostaglandins / Q000032:analysis* / Q000652:urine; D013189:SRS-A / Q000031:analogs & derivatives / Q000032:analysis; D013929:Thromboxane B2 / Q000032:analysis* / Q000652:urine
D015289:Leukotrienes; D011453:Prostaglandins; D013189:SRS-A; D007975:Leukotriene B4; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D017999:Leukotriene E4; D000527:Alprostadil; D015232:Dinoprostone