Sequencing and characterization of the sdaB gene from Escherichia coli K-12.
The sdaB gene which codes for the second L-serine deaminase (L-SD) of Escherichia coli K-12 has been sequenced and shown to be very similar to the sdaA gene which codes for the first L-serine deaminase. sdaB is transcribed in rich medium, particularly in the absence of glucose, and is under the control of catabolite activator protein. A mutation which established expression of the sdaB gene and synthesis of L-serine deaminase 2 in minimal medium has been demonstrated to result in a change in the ribosome-binding site of the sdaB gene.
Shao
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Biology Dept., Concordia University, Montreal, Quebec, Canada.
Newman
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EB
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D007527:Isoenzymes / Q000235:genetics*; D007700:Kinetics; D012695:L-Serine Dehydratase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003062:Codon; D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D007527:Isoenzymes; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D013912:Threonine; D012694:Serine; D001616:beta-Galactosidase; D012695:L-Serine Dehydratase