Dicistronic transcription units for gene expression in mammalian cells.
Dicistronic vectors utilizing the internal ribosomal entry site sequence of poliovirus as the intercistronic region were constructed for gene expression in mammalian cells. We have developed two monocistronic expression vectors which facilitate the creation of dicistronic expression plasmids. The dicistronic expression plasmids encode transcription units which allow the coordinated translation of the two genes. Using internal luciferase and secreted alkaline phosphatase, we show the correlated expression of both reporter genes and expression levels comparable to those achieved by the respective monocistronic expression vectors.
Eukaryotic Genetics, GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
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Dirks
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Wirth
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Hauser
D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004274:DNA, Recombinant; D015870:Gene Expression; D005814:Genes, Viral; D005818:Genetic Engineering; D005819:Genetic Markers; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics; D008322:Mammals; D008969:Molecular Sequence Data; D010957:Plasmids; D017955:Poliovirus / Q000235:genetics; D014176:Protein Biosynthesis; D015202:Protein Engineering; D012333:RNA, Messenger / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013539:Simian virus 40; D014162:Transfection; D015678:Viral Structural Proteins / Q000235:genetics
D004274:DNA, Recombinant; D005819:Genetic Markers; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins; D008156:Luciferases; D000469:Alkaline Phosphatase