Visualization of intracellular calcium during necrotaxis.
Granulocytes are known to exhibit positive chemotaxis towards erythrocytes destroyed by laser irradiation (necrotaxis). A computerized method for visualizing intracellular calcium concentration in granulocytes during necrotaxis has been developed. Successive bursts of fluorescence were observed: 1 degree) about 10 s after the creation of the necrotactic source; 2 degrees) when the granulocyte touched the target.
Constant
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Centre d'Ecologie Cellulaire, Hôpital de la Salpétrière, Paris, France.
Le Negrate
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de Boisfleury-Chevance
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D000703:Analog-Digital Conversion; D000814:Aniline Compounds; D002118:Calcium / Q000032:analysis*; D002451:Cell Compartmentation; D016923:Cell Death / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D004912:Erythrocytes / Q000528:radiation effects; D006098:Granulocytes / Q000737:chemistry / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D014743:Videotape Recording; D014966:Xanthenes
D000814:Aniline Compounds; D014966:Xanthenes; C059715:Fluo-3; D002118:Calcium