Activation of mitochondrial DNA replication by He-Ne laser irradiation.
To gain further insight into the effect of He-Ne laser irradiation on the mitochondrial biosynthetic apparatus, DNA synthesis was measured in mitochondria, mitoplasts and mitochondrial matrix fraction irradiated with low power continuous wave He-Ne laser (energy dose 5 Joules/cm2; laser power 12 mW). As a result of irradiation, the amount of alpha-[32P]dATP incorporated in acid insoluble materials was found to increase. Electrophoretic analysis of in vitro synthesised DNA purified from mitochondria, mitoplasts and matrix fraction shows an increase of 50-60% of DNA synthesis in the irradiated samples. These data show that He-Ne laser stimulates the replication of mitochondrial DNA in both intact organelles and soluble matrix fraction, thus suggesting an interaction of laser light with matrix soluble molecule/s.
Centro di Studio sui Mitocondrie e Metabolismo Energetico, C.N.R. Bari, Italy.
R A
RA
Vacca
E
E
Marra
E
E
Quagliariello
M
M
Greco
D000818:Animals; D004261:DNA Replication / Q000528:radiation effects*; D004272:DNA, Mitochondrial / Q000096:biosynthesis* / Q000302:isolation & purification; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D007700:Kinetics; D007834:Lasers; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism* / Q000528:radiation effects; D010761:Phosphorus Radioisotopes; D051381:Rats; D017208:Rats, Wistar
D004272:DNA, Mitochondrial; D003838:Deoxyadenine Nucleotides; D010761:Phosphorus Radioisotopes; C026600:2'-deoxyadenosine triphosphate