Human erythrocyte band 3 (EPB3) polymorphism: analysis of blood and bloodstains by an immunodetection method and frequency of the EPB3* Memphis variant.
The erythrocyte band 3 (EPB3) variant, band 3 Memphis (EPB3*Memphis), was detected by immunoblotting with a monoclonal antibody to the 41 kDa cytoplasmic N-terminal domain of band 3 without protease treatment of erythrocytes. EPB3*Memphis was also detected by immunoblotting from 3-month-old bloodstains subjected to alpha-chymotrypsin treatment. A population genetic study using this method indicated that the EPB3 variant would be useful for forensic work in Japan, since the frequency of this variant in Japanese (Wakayama prefecture) is relatively high (0.159).
Department of Legal Medicine, Wakayama Medical College, Japan.
A
A
Kimura
T
T
Uda
S
S
Nakashima
H
H
Ikeda
S
S
Yasuda
M
M
Osawa
T
T
Tsuji
D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics*; D001782:Blood Donors / Q000706:statistics & numerical data; D001801:Blood Stains; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D015151:Immunoblotting; D007564:Japan; D011110:Polymorphism, Genetic
D001457:Anion Exchange Protein 1, Erythrocyte; C070316:band 3 protein Memphis