Polymerase chain reaction (PCR) identification of human papillomavirus (HPV) DNA in verrucous carcinoma of the larynx.
The incidence of human papillomavirus (HPV) DNA in archival formalin-fixed, paraffin-embedded tissue sections from verrucous carcinoma of the larynx was determined using polymerase chain reaction (PCR) with consensus primers and by in situ hybridization designed to detect HPV types 6/11, 16/18, 31/33/35. HPV DNA was detected in 17 (85%) of 20 tissue samples by PCR; none of the 20 samples were positive for the seven genotype types tested by in situ hybridization. PCR is a valuable tool to detect HPV and therefore will significantly clarify the importance of HPV in squamous mucosal disorders.
Department of Otolaryngology, Mayo Clinic, Rochester, Minn. 55905.
J L
JL
Kasperbauer
G L
GL
O'Halloran
M J
MJ
Espy
T F
TF
Smith
J E
JE
Lewis
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002291:Carcinoma, Papillary / Q000382:microbiology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005784:Gene Amplification; D016679:Genome, Viral; D005914:Globins / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
D015342:DNA Probes; D004279:DNA, Viral; D005914:Globins