Cultivation of Plasmodium falciparum parasites in a serum-free medium.
The elimination of serum from Plasmodium falciparum culture media could decrease costs, enhance procurement, and improve the feasibility of large-scale production of parasite material. We provide a semi-defined, serum-free formulation, of commercially available constituents that supports P. falciparum parasite growth at rates comparable with those obtained with serum-supplemented media. The medium is composed of RPMI 1640 to which HEPES, extra glucose, bicarbonate, and hypoxanthine have been added. Bovine albumin and serum-derived, lipids-cholesterol-rich mixture are then used in place of serum.
Biomedical Sciences Research Center, Kenya Medical Research Institute, Nairobi.
A V
AV
Ofulla
V C
VC
Okoye
B
B
Khan
J I
JI
Githure
C R
CR
Roberts
A J
AJ
Johnson
S K
SK
Martin
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D016895:Culture Media, Serum-Free; D005947:Glucose / Q000378:metabolism; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D050356:Lipid Metabolism; D010963:Plasmodium falciparum / Q000254:growth & development*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D017693:Sodium Bicarbonate
D001639:Bicarbonates; D016895:Culture Media, Serum-Free; D007042:Hypoxanthines; D012710:Serum Albumin, Bovine; D019271:Hypoxanthine; D017693:Sodium Bicarbonate; D002784:Cholesterol; D012964:Sodium; D005947:Glucose