Lack of correlation between the gap junctional communication capacity of human colon cancer cell lines and expression of the DCC gene, a homologue of a cell adhesion molecule (N-CAM).
In many human colorectal cancers, the DCC gene encoding for a homologue of the neural cell adhesion molecule (N-CAM) is found to be deleted. Previous work suggested that gap junctional intercellular communication (GJIC) might play an important role in carcinogenesis and could be regulated by the expression of cell adhesion molecules such as E-cadherin in some epithelial cell systems. In order to examine whether the deletion of the putative cell adhesion molecule DCC is related to the level of GJIC, which might, in turn, be important in human colorectal cancers, we compared levels of expression of the DCC gene with the GJIC capacity of a panel of human colorectal adenocarcinoma cell lines isolated from different stages of tumor progression. While the level of GJIC varied between the cell lines studied, we found no correlation between their communication capacity and DCC expression revealed by a reverse-transcriptase/polymerase chain reaction method. This lack of correlation suggests that DCC is not a crucial regulator of GJIC.
Unit of Multistage Carcinogenesis, International Agency for Research on Cancer, Lyon, France.
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M
Mesnil
C
C
Piccoli
J L
JL
Klein
I
I
Morand
H
H
Yamasaki
D000230:Adenocarcinoma / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D001483:Base Sequence; D002450:Cell Communication / Q000502:physiology; D003110:Colonic Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D017630:Connexins; D015972:Gene Expression Regulation, Neoplastic; D016163:Genes, DCC; D006801:Humans; D007365:Intercellular Junctions / Q000502:physiology*; D008565:Membrane Proteins / Q000032:analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
D017630:Connexins; D008565:Membrane Proteins; D012333:RNA, Messenger