Expulsion of Echinostoma trivolvis (Cort, 1914) Kanev, 1985 and retention of E. caproni Richard, 1964 (Trematoda: Echinostomatidae) in C3H mice: pathological, ultrastructural, and cytochemical effects on the host intestine.
C3H mice were infected with 30 metacercarial cysts of either echinostome to study the pathological, ultrastructural, and cytochemical effects of the infection on the mouse small intestine. In mice infected with Echinostoma caproni, the intestine showed villous atrophy with fused or eroded villi. The microvilli of the enterocytes were sparse and distorted and showed reduced alkaline phosphatase activity. The crypts of Lieberkuhn were hyperplastic and showed a marked reduction in goblet and Paneth cells. As compared with uninfected controls, there was a marked reduction in glucose-6-phosphatase activity in the enterocytes of the infected gut. Collagen fibers and the number of fibroblasts were increased under the epithelium. In mice infected with E. trivolvis, the tips of the intestinal villi were bent and blunted. The microvilli of the enterocytes were less tightly packed than those of uninfected controls. The mitochondria in the enterocytes were irregularly shaped, contained intracristal bodies, and showed increased cytochrome oxidase activity as compared with those of uninfected controls. The crypts were hyperplastic but showed an increase in the numbers of goblet and Paneth cells. The fibroblasts and collagen fibers showed abnormal development. The ultrastructural and cytochemical differences seen in this study reflect the uniqueness of the host-parasite relationship of each of these echinostome species in the gut of the C3H mouse.
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
T
T
Fujino
B
B
Fried
D000818:Animals; D001284:Atrophy; D003094:Collagen / Q000648:ultrastructure; D004449:Echinostoma / Q000472:pathogenicity* / Q000648:ultrastructure; D004451:Echinostomiasis / Q000473:pathology*; D003576:Electron Transport Complex IV / Q000302:isolation & purification; D005260:Female; D005952:Glucose-6-Phosphatase / Q000302:isolation & purification; D006651:Histocytochemistry; D007422:Intestines / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H / Q000469:parasitology; D008871:Microvilli / Q000473:pathology / Q000648:ultrastructure; D008928:Mitochondria / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D013045:Species Specificity; D014774:Virulence
D003094:Collagen; D003576:Electron Transport Complex IV; D005952:Glucose-6-Phosphatase