Effects of pyrroloquinoline quinone (PQQ) and PQQ-oxazole on DNA synthesis of cultured human fibroblasts.
The effects of pyrroloquinoline quinone (PQQ) and PQQ-oxazole (PQQ-glycine adduct) on DNA synthesis were examined using cultured human fibroblasts. Confluent fibroblasts were cultured in serum-free Dulbecco's modified Eagle's media, and various concentrations of PQQ and PQQ-oxazole were added to the media. After incubation for 24 h, [3H]thymidine was added to the media as an indicator for DNA synthesis of the cells. The thymidine incorporation into the cells was significantly enhanced even in the presence of very low concentrations of PQQ (0.003-0.03 microM); it remained significantly enhanced, up to 30 microM PQQ. However, the incorporation remarkably decreased at 750-1500 microM of PQQ. In contrast to the results for PQQ, DNA synthesis was not stimulated by addition of 0.003-3 microM PQQ-oxazole, but it was slightly enhanced at concentrations 15-750 microM. In morphological examination of the cultured human fibroblasts, cell density was increased by addition of 0.003-30 microM PQQ when compared with that of the control, supporting the above biochemical data. However, there were no distinct differences in morphological effect between PQQ and PQQ-oxazole.
Department of Pathology, Hamamatsu University School of Medicine, Japan.
Y
Y
Naito
T
T
Kumazawa
I
I
Kino
O
O
Suzuki
D000328:Adult; D002460:Cell Line; D003067:Coenzymes / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D010080:Oxazoles / Q000494:pharmacology*; D045542:PQQ Cofactor; D011804:Quinolines / Q000494:pharmacology*; D015363:Quinolones / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D003067:Coenzymes; D010080:Oxazoles; D011804:Quinolines; D015363:Quinolones; C081512:pyrroloquinoline quinone-oxazole; D045542:PQQ Cofactor; D004247:DNA; D013936:Thymidine