Clinical and analytical evaluation of a continuous enzymatic method for measuring pancreatic lipase activity.
We report the evaluation of a new commercial kit for the determination of pancreatic lipase activity. The kit is based on the use of a 1,2-diglyceride as substrate and a specific monoglyceride lipase. The detection step is the continuous colorimetric measurement of hydrogen peroxide produced from glycerol by glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase reactions. The procedure appears to be precise (between-day CV < 9%) and the results show good correlation with those obtained by alternative procedures (vs turbidimetry, r = 0.965; vs ultraviolet absorbance-enzymatic method, r = 0.995; vs Ektachem, r = 0.976; vs immunometry, r = 0.970). However, the method is susceptible to interference by increased concentrations (> 4.5 mmol/L) of serum triglycerides. We estimated the reference interval for healthy adults to be 8-44 U/L. When we evaluated clinical efficacy by using receiver-operating characteristic curves and the overlap index, no significant differences were found between the commercial kit and a common turbidimetric assay for diagnosing patients with acute pancreatitis; both methods performed satisfactorily.
Laboratorio Analisi Chimico-Cliniche, Spedali Civili, Brescia, Italy.
M
M
Panteghini
F
F
Pagani
R
R
Bonora
D000328:Adult; D000368:Aged; D003124:Colorimetry; D004075:Diglycerides / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005260:Female; D005991:Glycerol Kinase; D005993:Glycerolphosphate Dehydrogenase; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D007202:Indicators and Reagents; D007223:Infant; D008049:Lipase / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D010179:Pancreas / Q000201:enzymology*; D010195:Pancreatitis / Q000201:enzymology; D009195:Peroxidase; D011933:Reagent Kits, Diagnostic / Q000592:standards* / Q000706:statistics & numerical data; D012016:Reference Values
D004075:Diglycerides; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D006861:Hydrogen Peroxide; D005993:Glycerolphosphate Dehydrogenase; D009195:Peroxidase; D005991:Glycerol Kinase; D008049:Lipase