Preliminary crystallographic analysis of coxsackievirus A9.
Coxsackievirus A9 has been crystallized as small rhombic dodecahedra of maximum dimension 0.3 mm. These crystals have been shown, using synchrotron radiation, to diffract X-rays to beyond 3 A, and to have a stability in the beam comparable to that of other related virus crystals. The unit cell is tetragonal with dimensions a = b = 495 A, c = 695 A and alpha = beta = gamma = 90 degrees, with a space group of P4n22. A substantial body of diffraction data has been collected and this crystal form appears to be suitable for structure determination. Phasing of these data will be attempted using molecular replacement.
Smyth
M
M
Laboratory of Molecular Biophysics, Oxford, U.K.
Hall
J
J
Fry
E
E
Stuart
D
D
Stanway
G
G
Hyypiä
T
T
D000818:Animals; D002460:Cell Line; D003460:Crystallization; D004770:Enterovirus / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification; D014961:X-Ray Diffraction / Q000379:methods