Immunohistochemical detection of aflatoxin B1-DNA adducts and hepatitis B virus antigens in hepatocellular carcinoma and nontumorous liver tissue.
Monoclonal antibodies recognizing the stable imidazole ring-opened form of the major N7-guanine aflatoxin B1-DNA adduct have been used in competitive enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays to quantitate adduct levels in liver tissue. Methods were developed in AFB1-treated animals, then applied to paired tumor and nontumor liver tissues of hepatocellular carcinoma patients from Taiwan. An avidin-biotin complex staining method was also used for of the detection of hepatitis B surface (HBsAg) and X (HBxAg) antigens in liver sections. A total of 8 (30%) hepatocellular carcinoma (HCC) samples and 7 (26%) adjacent nontumor liver tissue samples from Taiwan were positive for AFB1-DNA adducts. For HBsAg, 10 (37%) HCC samples and 22 (81%) adjacent nontumorous liver samples were positive, and 9 (33%) HCC samples and 11 (41%) adjacent nontumor liver samples were HBxAg positive. No association with AFB1-DNA adducts was observed for HBsAg and HBxAg. These methods should be useful in determining the role of exposure in the induction of HCC in Taiwan.
Comprehensive Cancer Center, School of Public Health, Columbia University, New York, NY 10032.
R M
RM
Santella
Y J
YJ
Zhang
C J
CJ
Chen
L L
LL
Hsieh
C S
CS
Lee
B
B
Haghighi
G Y
GY
Yang
L W
LW
Wang
M
M
Feitelson
D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000382:microbiology; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006511:Hepatitis B Antigens / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism / Q000382:microbiology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000382:microbiology; D051381:Rats; D015534:Trans-Activators / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins
D018736:DNA Adducts; D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C072611:aflatoxin B1-DNA adduct; C057628:hepatitis B virus X protein; D004247:DNA; D016604:Aflatoxin B1