Establishing an experimental infection model for peritoneal dialysis: effect of inoculum and volume.
The effect of the number of bacteria and the volume of the inoculum was studied in an experimental infection model to establish a peritoneal dialysis model in the rat. Staphylococcus aureus was used in all experiments, and Staphylococcus epidermidis only in the volume experiments. A bacterial number between 10(8) and 10(9) colony-forming units (cfu) resulted in a time-dependent decrease of bacteria collected from the peritoneal cavity. Higher concentrations resulted in the death of animals, while lower concentrations were rapidly cleared. There was a positive correlation between the volume in which 3 x 10(8) cfu were dissolved and the number of bacteria isolated from the peritoneal cavity 24 hours after infection. The results of this study led to an experimental dialysis model using 10 mL of dialysis fluid and 0.5 mL of a suspension containing 3 x 10(8) cfu of Staphylococcus aureus.
Department of Cell Biology, Vrije Universiteit, Amsterdam, The Netherlands.
W
W
Calame
C
C
Afram
N
N
Blijleven
R J
RJ
Hendrickx
F
F
Namavar
R H
RH
Beelen
D000818:Animals; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D008297:Male; D010529:Peritoneal Cavity / Q000382:microbiology; D010530:Peritoneal Dialysis / Q000009:adverse effects*; D010538:Peritonitis / Q000209:etiology* / Q000382:microbiology; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000209:etiology* / Q000382:microbiology; D013211:Staphylococcus aureus; D013212:Staphylococcus epidermidis