Antibodies raised against yeast HSP 104 cross-react with a heat- and abscisic acid-regulated polypeptide in rice.
Antibodies raised against yeast heat shock protein (HSP) 104 recognized a heat-inducible polypeptide with a molecular mass of 110 kDa in shoot tissue of young rice seedlings. Root tissue of the same age showed no immuno-reaction with yeast HSP 104 antibodies. The 110 kDa polypeptide of rice was also shown to be abscisic acid-inducible in young seedlings. Though this polypeptide was seen to be constitutively present in the flag leaf of 90-day-old field-grown plant, it was not much affected by either heat shock or abscisic acid in this case.
Singla
S L
SL
Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
Grover
A
A
D000040:Abscisic Acid / Q000378:metabolism*; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000276:immunology*; D006360:Heat-Shock Proteins / Q000276:immunology*; D006358:Hot Temperature; D012275:Oryza / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D029701:Saccharomyces cerevisiae Proteins
D000906:Antibodies; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; C070972:HsP104 protein, S cerevisiae; D000040:Abscisic Acid