Functional analysis of the human IGF-I gene promoters.
The two putative promoter regions of the human insulin-like growth factor-I (IGF-I) gene, located upstream of the two alternatively used leader exons 1 and 2, were tested for promoter activity in transient transfection assays. Both regions were shown to possess promoter activity. The results further indicate cell-type-specific regulation of the activities of the two promoters in endogenously IGF-I producing human cell lines of different origin.
Steenbergh
P H
PH
Laboratory for Physiological Chemistry, University of Utrecht, The Netherlands.
Jansen
E
E
van Schaik
F M
FM
Sussenbach
J S
JS
D000818:Animals; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D014162:Transfection; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D008156:Luciferases