Cloning and functional expression of a human neuroendocrine vasoactive intestinal peptide receptor.
Vasoactive intestinal peptide (VIP) is a potent mediator of gastrointestinal, nervous, pulmonary, vascular and immune functions. A cDNA was obtained from human HT29 intestinal epithelial cells and found to encode a 457 amino acid, 52 kDa VIP receptor. Transfection of the cDNA into COS-7 cells and 293 cells resulted in expression of specific saturable binding of VIP with a Kd of 0.8 nM, and induction of increases in intracellular cAMP by VIP with an EC50 of 1 nM. The human VIP receptor is homologous to other G protein-coupled receptors of the secretin-parathyroid hormone receptor family. A 2.8 kb transcript was detected in human lung, HT29 cells and Raji B-lymphoblasts with weaker expression in human brain, heart, kidney, liver and placenta.
Department of Medicine, University of California, San Francisco 94143-0724.
S P
SP
Sreedharan
D R
DR
Patel
J X
JX
Huang
E J
EJ
Goetzl
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D015870:Gene Expression; D005910:Glioma; D006801:Humans; D006822:Hybrid Cells; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011964:Receptors, Gastrointestinal Hormone / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018005:Receptors, Vasoactive Intestinal Peptide; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism* / Q000494:pharmacology
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D011994:Recombinant Proteins; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP