Study of phosphorylation of translation elongation factor 2 (EF-2) from wheat germ.
Phosphorylation of elongation factor 2 (EF-2) by specific Ca2+/calmodulin-dependent kinase is considered as a possible mechanism of regulation of protein biosynthesis in animal cells at the level of polypeptide chain elongation. In this report we show that wheat germ EF-2 can be intensively phosphorylated by the rabbit reticulocyte EF-2 kinase. Phosphorylation results in inhibition of the activity of plant EF-2 in poly(U)-dependent cell-free translation system. Thus, the activity of EF-2 in plant cells can be potentially regulated by phosphorylation. However, we could not detect endogenous EF-2 kinase activity in wheat germ either in vitro or in vivo. Furthermore, EF-2 kinase activity is not displayed in different organs of wheat and other higher plants.
Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Alma-Ata, Kazakhstan.
S K
SK
Smailov
A V
AV
Lee
B K
BK
Iskakov
D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002474:Cell-Free System; D015180:Electrophoresis, Gel, Two-Dimensional; D054736:Elongation Factor 2 Kinase; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000302:isolation & purification / Q000378:metabolism*; D010455:Peptides; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014268:Trifluoperazine / Q000494:pharmacology; D014908:Triticum / Q000378:metabolism*
D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D010455:Peptides; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D014268:Trifluoperazine; D011072:Poly U; C012001:polyphenylalanine; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D054736:Elongation Factor 2 Kinase; D010744:Phosphoric Monoester Hydrolases