Detection of aluminium(III) binding to citrate in human blood plasma by proton nuclear magnetic resonance spectroscopy.
Reactions of Al3+ (50-500 mumol l-1) with intact blood plasma and its low relative molecular mass ultrafiltrate (< 5 kDa) have been studied by proton nuclear magnetic resonance spectroscopy. Binding to citrate was detected and was reversed by addition of desferrioxamine. The use of combined exponential and sine-bell functions for the resolution enhancement of spectra of plasma is illustrated.
NMR Unit, Hammersmith Hospital, London, UK.
J D
JD
Bell
G
G
Kubal
S
S
Radulovic
P J
PJ
Sadler
A
A
Tucker
D000535:Aluminum / Q000097:blood*; D002951:Citrates / Q000097:blood*; D003676:Deferoxamine / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010949:Plasma / Q000737:chemistry; D011522:Protons
D002951:Citrates; D011522:Protons; D000535:Aluminum; D003676:Deferoxamine