Inactivation of viruses during ultraviolet light treatment of human intravenous immunoglobulin and albumin.
A comparison of ultraviolet (UV) irradiation of two wavelength ranges UVB (280-320 nm) and UVC (lower than 280 nm) showed that UVC in particular could very effectively inactivate, in intravenous immunoglobulin (IVIG) and albumin preparations, non-enveloped and non-acid labile model viruses (i.e., Polio 2 and T4 phage) and dry heat-resistant viruses (vaccinia and T4 phage). This effective virucidal treatment (5 min, 5,000 J/m2 dose) was achieved before an unacceptable level of IVIG aggregates occurred. The use of UV irradiation to inactivate infectious agents could add safety and supplement current methods, e.g. solvent/detergent, low pH, which do not inactivate non-enveloped, non-acid labile or dry-heat-resistant viruses at present.
Protein Fractionation Centre, Scottish Blood Transfusion Service, Edinburgh.
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D000914:Antibodies, Viral / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000528:radiation effects*; D007610:Kallikreins / Q000528:radiation effects; D017955:Poliovirus / Q000528:radiation effects; D011494:Protein Kinases / Q000528:radiation effects; D011836:Radiation Tolerance; D012672:Semliki forest virus / Q000528:radiation effects; D012709:Serum Albumin / Q000528:radiation effects*; D018139:Simplexvirus / Q000528:radiation effects; D013604:T-Phages / Q000528:radiation effects; D014466:Ultraviolet Rays; D014616:Vaccinia virus / Q000528:radiation effects; D014780:Viruses / Q000528:radiation effects*
D000914:Antibodies, Viral; D016756:Immunoglobulins, Intravenous; D012709:Serum Albumin; D011494:Protein Kinases; D007610:Kallikreins