[Effect of Na-L-glutamate on mitochondrial function of ATP synthesis after prolonged preservation in rat heart].
We studied the effect of Na-L-glutamate (Glu) on mitochondrial functions of ATP synthesis after prolonged hypothermic preservation of rat hearts. We divided isolated rat hearts into the following three groups: group A, hearts without immersion as control; group B, hearts immersed in Euro-Collins solution (E-C) without Glu for 24 h at 4 degrees C; group C, hearts immersed in E-C with 10mM Glu for 24 h at 4 degrees C. Number of each group used was 6. Mitochondrial functions of ATP production were determined by measuring respiratory control index (RCI). P/O ratio and state III respiration rate. The ability of ATP production (oxidative phosphorylation rate, OPR) was calculated. RCI and P/O ratio of groups B and C were significantly lower than those of group A. RCI and P/O ratio of group C were significantly higher than that of group B. S3 rates of groups B and C were significantly lower than those of group A. S4 rate of group B was significantly higher than those of groups A and C. OPRs of groups B and C were significantly lower than that of group A. OPR of group C was significantly higher than that of group B. We concluded that Na-L-glutamate effectively protected mitochondrial functions to synthesize ATP during prolonged hypothermic preservation.
Second Department of Surgery, Miyazaki Medical College, Japan.
M
M
Kuwabara
T
T
Onitsuka
K
K
Shibata
Y
Y
Koga
M
M
Hamada
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D006321:Heart; D066298:In Vitro Techniques; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D009926:Organ Preservation; D051381:Rats; D017208:Rats, Wistar; D012970:Sodium Glutamate / Q000494:pharmacology*; D013997:Time Factors
D000255:Adenosine Triphosphate; D012970:Sodium Glutamate