Regulation of fibronectin mRNA expression in primary hepatocytes in response to EGF and phenobarbital.
Adult rat hepatocytes were cultured in serum free chemically defined MX-83 medium. Fibronectin mRNA expression accumulated to high levels in primary cultures of hepatocytes and transcripts were detectable even after 2 hrs. However, the presence of phenobarbital (PB) in the culture medium led to a dose-dependent decrease in fibronectin mRNA levels concomitant to an increase of cytochrome P450-2B1 (CYP2B1) mRNA, whereas the coaddition of EGF reversed the suppressive effect of PB in a similar dose-dependent manner. Culturing hepatocytes on a fibronectin matrix partially inhibited the accumulation of cytochrome CYP2B-dependent pentoxyresorufin-O-depentylase (PROD) activities in the presence of the inductor PB.
Institute of Pharmacology and Toxicology, University of Göttingen, Germany.
J
J
Aubrecht
G F
GF
Kahl
M W
MW
Höhne
D000818:Animals; D019362:Cytochrome P-450 CYP2B1; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000502:physiology; D005353:Fibronectins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D066298:In Vitro Techniques; D016023:Integrins / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000378:metabolism; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin / Q000378:metabolism*; D017474:Receptors, Laminin / Q000378:metabolism
D005353:Fibronectins; D016023:Integrins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin; D017474:Receptors, Laminin; D004815:Epidermal Growth Factor; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019362:Cytochrome P-450 CYP2B1; D010634:Phenobarbital