A simplified method of pig islet isolation.
This study reports a simplified, low-cost, easily performed chemico-mechanical isolation method of the islets of Langerhans. The experimental study was carried out on 15 adult pigs. Our method used collagenase and a digestion chamber for islet isolation and yielded 2,240 +/- 613 islets per g treated pancreas after purification. This demonstrates that the method is reliable and can give satisfactory results.
Carnazzo
S A
SA
Clinica Chirurgica I, Università di Catania, Italy.
Lanteri
R
R
Petino
A G
AG
Favetta
A
A
Distefano
A M
AM
Stivala
F
F
Latteri
E
E
D000818:Animals; D002469:Cell Separation / Q000191:economics / Q000295:instrumentation / Q000379:methods; D005069:Evaluation Studies as Topic; D005260:Female; D007515:Islets of Langerhans / Q000166:cytology*; D013552:Swine