Engraftment of bovine leukemia virus infected lymphocytes into SCID mice.
SCID mice inoculated with peripheral blood lymphocytes from a tumor-bearing BLV-positive cow developed lymphosarcomas after 6 months. DNA from the splenic tumor specifically hybridized with a bovine Sstl satellite probe, showing that these tumor cells were of bovine origin. The clonality of the SCID splenic tumor, as measured by the integration pattern of BLV proviruses, was the same as that in the donor lymphocyte population.
Department of Microbiology, Immunology, and Parasitology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
L
L
Haas
J
J
Perdrizet
M
M
Butt
M
M
Posso
F
F
Quimby
J W
JW
Casey
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000382:microbiology; D008228:Lymphoma, Non-Hodgkin / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D013160:Splenic Neoplasms / Q000382:microbiology* / Q000473:pathology
D004273:DNA, Neoplasm; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides