Serological and biochemical detection of B-cell antigens in goats.
The production of B-cell specific alloantisera by lymphocyte transfusion between class I matched, MLR positive goats is described. Furthermore, the usefulness of preceding IEF typing is demonstrated in the selection of immunization pairs. After suitable absorptions and cluster analysis the detected B-cell specific antigens were designated BeD1-BeD6. The specificity BeD3 could also be detected by two class II-specific murine anti-H2 monoclonal antibodies. IEF class II typing of 34 animals gave concordant results between the two techniques. One additional allelic variant could be detected by IEF typing. The detected products segregated in close linkage to the earlier described caprine class I antigens. One recombinant has been found in 78 offspring. The reactivity of cells in mixed lymphocyte cultures (MLC) was correlated with the compatibility of the test cells for their B-cell specific antigens. Three of the B-cell specific sera were characterized by immunoprecipitation. The precipitated antigens corresponded in molecular weight to MHC class II products as described in other species indicating that the here described caprine products are of class II nature.
Ruff
G
G
Institute of Animal Breeding, School of Veterinary Medicine, University of Berne, Switzerland.
Joosten
I
I
Howald
M
M
Davis
W C
WC
Lazary
S
S
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis* / Q000235:genetics; D003429:Cross Reactions; D005260:Female; D006041:Goats / Q000235:genetics / Q000276:immunology*; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006650:Histocompatibility Testing; D007114:Immunization; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000096:biosynthesis; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D008297:Male
D000944:Antigens, Differentiation, B-Lymphocyte; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies