[Establishment and characterization of a new human testicular germ cell tumor cell line (TCam-2)].
A new human testicular cancer cell line (TCam-2) was established. The original material of TCam-2 was a primary lesion of a left testicular seminoma (typical pure type seminoma) from a 35 aged male patient. TCam-2 produced neither AFP nor beta-HCG, It showed strong immunoreactivities for 5G9 (anti testicular cancer MoAb), 4B3 (anti PTHrP MoAb) and PALP (placental alkaline phosphatase). The chromosomal analysis revealed 92 modal number and loss of Y chromosome. Histochemical, morphological and chromosomal analysis supported that TCam-2 is from classical seminoma. TCam-2 was transplanted subcutaneously to the back of 6 weeks old scid mice (CB-17 strain), and grew a classical seminomatous tissue.
Department of Urology, Kobe University School of Medicine.
Y
Y
Mizuno
A
A
Gotoh
S
S
Kamidono
S
S
Kitazawa
D000328:Adult; D000818:Animals; D004407:Dysgerminoma / Q000473:pathology; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics / Q000473:pathology*; D013736:Testicular Neoplasms / Q000235:genetics / Q000473:pathology*