Differential induction of glutathione S-transferase subunits by phenobarbital, 3-methylcholanthrene and ethoxyquin in rat liver and kidney.
The inducibility of Glutathione S-transferase (GST) in male Sprague-Dawley rats, treated with phenobarbital (PB), 3-methyl-cholanthrene (MC) and ethoxyquin (ETQ), was examined in detail. The subunit compositions of hepatic and renal GST were determined by using a reverse-phase HPLC technique. In liver, PB was found to induce the Yb1, Yb2, Ya1, Ya2 and Yk subunits by about 2.1-, 1.8-, 1.8-, 4.4- and 2-fold, respectively, while MC induced the Yb2, Yc, Ya2 and Yk subunits by about 1.5-, 1.5-, 6- and 1.7-fold, respectively, and ETQ increased the levels of Yb1, Yb2, Yc, Ya2 and Yk subunits by about 2.1-, 1.7-, 1.9-, 14.9- and 1.8-fold, respectively. In contrast, kidney cytosolic GSTs were induced only by treatment with ETQ and PB and MC had little or no effect. The Pi class subunit Yp in the rat kidney was increased about 4-fold and the Mu class Yb2 was induced by about 2-fold, by the ETQ treatment.
Derbel
M
M
Laboratory of Biochemical Pharmacology and Biotoxicology, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Igarashi
T
T
Satoh
T
T
D000818:Animals; D004790:Enzyme Induction; D005015:Ethoxyquin / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000737:chemistry; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008748:Methylcholanthrene; D005015:Ethoxyquin; D005982:Glutathione Transferase; D010634:Phenobarbital