Regulation of phosphatidylcholine synthesis in fetal type II cells by CTP:phosphocholine cytidylyltransferase.
Phosphatidylcholine synthesis increases in fetal rat type II cells during late gestation, as demonstrated by an increased incorporation of radiolabeled palmitate, glycerol, acetate, and choline into phosphatidylcholine. However, the percentage of phosphatidylcholine present in the saturated form remains essentially constant. The developmental profile of the enzymes of the CDP-choline pathway suggests that CTP:choline-phosphate cytidylyltransferase catalyses a rate regulatory step in de novo phosphatidylcholine synthesis by fetal type II cells. When cytidylyltransferase activity is assayed in different subcellular fractions, the greatest increase, as a function of development, is found in microsomes. This developmental increase is accompanied by a shift in subcellular distribution of cytidylyltransferase activity from cytosol to microsomes in fetal type II cells during late gestation. This shift is evident even when cytidylyltransferase activity is assayed in the presence of 0.5 mM phosphatidylcholine/oleic acid (1/1 molar ratio) vesicles. We speculate that either a subcellular translocation of CTP:phosphocholine cytidylyltransferase from cytosol to microsomes or an increase in cytidylyltransferase gene expression are responsible for the developmental increase of de novo phosphatidylcholine synthesis by fetal type II cells.
Department of Pediatrics, Hospital for Sick Children Research Institute, Toronto, Ontario, Canada.
L J
LJ
Zimmermann
M
M
Hogan
K S
KS
Carlson
B T
BT
Smith
M
M
Post
D000818:Animals; D019916:Choline-Phosphate Cytidylyltransferase; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis; D005333:Fetus / Q000166:cytology / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000502:physiology*; D010713:Phosphatidylcholines / Q000096:biosynthesis* / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014018:Tissue Distribution
D004075:Diglycerides; D010713:Phosphatidylcholines; D003566:Cytidine Diphosphate Choline; D009713:Nucleotidyltransferases; D019916:Choline-Phosphate Cytidylyltransferase