Transcription of Drosophila mobile element gypsy (mdg4) in heat-shocked cells.
Drosophila melanogaster Schneider 2 line cultured cells were subjected to stable transformation by co-transfection with two plasmids, one of which conferred G418 resistance and another which contained the Drosophila retrotransposon, gypsy (mdg4), under the control of the heat-shock protein 70 promoter. Transcription of the introduced constructs, as well as of endogenous gypsy, was examined under the condition of heat shock. Active degradation of pre-existing gypsy transcripts was observed. During recovery, gypsy transcription was restored, but its termination and/or 3'-end processing became aberrant.
V.A. Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Moscow.
N V
NV
Lyubomirskaya
I R
IR
Arkhipova
Ilyin YuV
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics; D006358:Hot Temperature; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D004251:DNA Transposable Elements; D006360:Heat-Shock Proteins