Expression of the beta 3-adrenergic receptor in human white adipose tissue.
The results of this study suggest that the atypical beta-adrenergic receptor (beta 3 subtype) is expressed in human white adipose tissue. A beta 3-adrenergic receptor mRNA could be detected in human omental fat poly(A)+ RNA by polymerase chain reaction amplification with appropriate primers. The expression of the beta 3-adrenergic receptor in human fat was confirmed by Northern blot analysis; however, the level of its mRNA was lower than those of the beta 1- and beta 2-adrenergic receptors. Two populations of ((-)-4-(3-t-butylamino-2-hydroxypropoxy)-[5,7-3H]benzimidazole-2-one) ([3H]CGP 12177)-binding sites were identified in human omental fat membranes, one with a high affinity (Kd = 1.7 nM) and the other with a low affinity (Kd = 22 nM). The low affinity binding site population, which should represent the beta 3-adrenergic receptor, was predominant (75% of the total binding sites).
Revelli
J P
JP
Département de Biochimie Médicale, Centre Médical Universitaire, Geneva, Switzerland.
Muzzin
P
P
Paoloni
A
A
Moinat
M
M
Giacobino
J P
JP
D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D004277:DNA, Single-Stranded; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009852:Omentum; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis* / Q000235:genetics
D004277:DNA, Single-Stranded; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta