Monoclonal antibodies to human polymorphonuclear leukocyte gelatinase (type IV collagenase) are cross-reactive with fibroblast gelatinase.
Four monoclonal antibodies to human polymorphonuclear leukocyte gelatinase, 100 kDa type IV collagenase, 1G5, 4C6, 4G6 and 4H4 clones, were generated by immunizing Balb/c mice and fusing the mouse spleen cells with P3U1 cells. By using the enzyme-linked immunosorbent assay method, two clones, 4G6 and 1G5, were found to cross-react with human fibroblast 75kDa gelatinase, although none of the four antibodies inhibited gelatinase activity. Selective detection of 75 kDa and 100 kDa gelatinases produced by human skin fibroblasts and fibrosarcoma cells (HT 1080) in culture was successfully demonstrated by immunostaining with combined uses of 4H4 and 4G6 clones.
Department of Tissue Physiology, Tokyo Medical and Dental University, Japan.
T
T
Kobayashi
H
H
Hori
T
T
Kanamori
S
S
Hattori
T
T
Takagi
H
H
Watanabe
T
T
Nishikawa
Y
Y
Nagai
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D017364:Collagenases / Q000032:analysis / Q000097:blood* / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D018093:Gelatinases; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology; D007700:Kinetics; D020780:Matrix Metalloproteinase 9; D008970:Molecular Weight; D009504:Neutrophils / Q000201:enzymology*; D012867:Skin / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D010450:Endopeptidases; D017364:Collagenases; D018093:Gelatinases; D020780:Matrix Metalloproteinase 9