[The 31P-NMR spectroscopic study of phosphate metabolism of perfused rabbit ovary in ovulation].
The phosphate metabolites in perfused rabbit ovary were measured during ovulation by 31P-NMR spectroscopy after the administration of PMSG and HCG. The c-AMP levels in perfusate from opposite ovarian veins were also measured by RIA. The results obtained were as follows: (1) The ovulatory ATP level significantly decreased compared with the preovulatory ATP level. After ovulation, the ATP level gradually recovered to the control level. On the other hand, both neither the Pi nor the PME level changed significantly from the preovulatory until the preluteal phase and recovered to the control level after the midluteal phase. (2) ATP levels rapidly decreased dose-dependently after the administration of HCG during ovulation. The ATP level in particular decreased significantly to 62.4% and 51.4% of the control level at 2 and 10 hours, respectively during the infusion of 10 IU/min of HCG. However, no significant change in either the Pi or the PME level was observed until the end of perfusion. (3) The concentration of c-AMP in the perfusate significantly increased dose-dependently after the administration of HCG. The concentration of c-AMP reached its peak level at 30 minutes after 10 IU/min HCG infusion from control level, and gradually decreased in the 10 hours after HCG infusion. These results suggest that the decrease in ATP during ovulation may reflect the increase in ATP consumption in the c-AMP dependent metabolic pathway in the ovary.
Mizukami
A
A
Department of Obstetrics and Gynecology, Asahikawa Medical College.
Tanaka
K
K
Ishikawa
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D010053:Ovary / Q000378:metabolism* / Q000502:physiology; D010060:Ovulation; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism*; D011817:Rabbits
D006063:Chorionic Gonadotropin; D010710:Phosphates; D000255:Adenosine Triphosphate; D000242:Cyclic AMP