Construction of site-specific mutants of E5A protein of HPV-11 using the polymerase chain reaction and a single mutant primer.
The method of Perrin and Gilliland (1990) was modified to create site-specific mutants. The polymerase chain reaction and a single mutant primer are needed to carry out site-specific mutagenesis. Using this method, removal of the excess primers and nucleotides from the initial amplification is not necessary. This method provides a simpler way to generate site-specific mutants.
Chen
S L
SL
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan.
Han
C P
CP
Tsao
Y P
YP
D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
C081917:E5A protein, Human papillomavirus type 11; D009856:Oncogene Proteins, Viral