Effect of alginate and calcium on secretion of IL-1 by immobilized Swiss peritoneal macrophages.
Murine peritoneal macrophages were immobilized in calcium alginate gel macrobeads, using 1.5% Na-alginate and 50 mM CaCl2. Secretion of IL-1 by immobilized macrophages increased during time and reached 10 fold than IL-1 quantities secreted by adherent macrophages. Calcium and alginate individually enhance production of IL-1 by macrophages and act in synergy when macrophages are immobilized in calcium alginate matrix.
Tahri Jouti
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MA
Laboratoire Interdisciplinaire de Recherche en Biologie Appliquée (L.I.R.B.A.) E.N.S. de Cachan, France.
Ramdi
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Lièvremont
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D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002214:Capsules; D002469:Cell Separation; D007375:Interleukin-1 / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008567:Membranes, Artificial; D051379:Mice; D008809:Mice, Inbred C3H; D013047:Specific Pathogen-Free Organisms; D013950:Thymus Gland / Q000166:cytology
D000464:Alginates; D002214:Capsules; D007375:Interleukin-1; D008567:Membranes, Artificial; D002118:Calcium