Elastin peptide concentration in human serum: variation with antibodies and elastin peptides used for the enzyme-linked immunosorbent assay.
Discrepancies exist between the reported values for the mean elastin peptide (EP) concentration in human sera. In order to understand these discrepancies, several EP preparations were obtained in vitro and monoclonal and polyclonal antibodies were produced against them. These different EP preparations and antibodies were used in an enzyme-linked immunosorbent assay (ELISA) to study cross-reactivity between EP preparations and to quantitate EP concentration in human sera. The method of purification of elastin, the method of hydrolysis of elastin and the molecular weight of EP influence their reactivity with antibodies and the results of EP measurements in human sera. However, there is a good correlation between EP measurements carried out in several human sera with the different EP preparations and different antibodies. Although absolute values of the EP concentrations varied with the EP preparation and antibodies used for the ELISA, the variations of this EP concentration measured from one human serum to another are significant.
Laboratoire de Biologie du Tissu Conjonctif, UA CNRS 1460, Faculté de Médecine, Créteil, France.
S M
SM
Wei
J
J
Erdei
T
T
Fülöp
L
L
Robert
M P
MP
Jacob
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001011:Aorta / Q000276:immunology; D003429:Cross Reactions; D004549:Elastin / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008168:Lung / Q000276:immunology; D008970:Molecular Weight; D010455:Peptides / Q000097:blood / Q000737:chemistry / Q000276:immunology; D011651:Pulmonary Artery / Q000276:immunology
D000911:Antibodies, Monoclonal; D010455:Peptides; D004549:Elastin