Endogenous superantigen expression controlled by a novel promoter in the MMTV long terminal repeat.
Endogenous superantigens are encoded by the open reading frame contained within the mouse mammary tumour virus long terminal repeat (MMTV LTR). Superantigen expression results in T-cell proliferation and, during early ontogeny, T-cell deletion. Here we identify a novel promoter located upstream of the previously described MMTV promoter. Transcripts from this promoter initiate within the U3 region of the MMTV LTR and splice to the acceptor for endogenous superantigen coding region. The novel U3 promoter is active in B lymphocytes, which are cognate antigen-presenting cells for endogenous superantigen, and is able to direct expression of superantigen in the absence of the previously described MMTV promoter.
GSF-Research Centre for Environment and Health, Institute of Molecular Virology, Oberschleissheim, Germany.
W H
WH
Günzburg
F
F
Heinemann
S
S
Wintersperger
T
T
Miethke
H
H
Wagner
V
V
Erfle
B
B
Salmons
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001402:B-Lymphocytes / Q000382:microbiology; D001483:Base Sequence; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D008321:Mammary Glands, Animal / Q000382:microbiology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic
D000956:Antigens, Viral