IS870 requires a 5'-CTAG-3' target sequence to generate the stop codon for its large ORF1.
The TB regions of the Agrobacterium vitis octopine/cucumopine Ti plasmids constitute a family of related structures. All contain a bacterial insertion element downstream of the TB-iaaM gene, IS870.1. Whereas 43 isolates with octopine/cucumopine Ti plasmids carry only one IS870 copy, strain Ag57 carries a second copy (IS870.2) 3.9 kb to the right of IS870.1 and part of the same TB region. Two other octopine/cucumopine strains carry an IS870 copy on their chromosome (IS870.3). A study of the unmodified insertion sites of IS870.2 and IS870.3, cloned from closely related strains, enabled us to delimit the IS870 elements. IS870 has a size of 1,152 bp and is terminated by inverted repeats. It contains a large open reading frame without a stop codon. However, a stop codon is generated by insertion into the target sequence 5'-CTAG-3'. IS870 is related to five other insertion sequence elements. For two of these, the stop codon of the largest open reading frame is also created by insertion into a CTAG target site.
Fournier
P
P
Institute of Plant Molecular Biology, Centre National de la Recherche Scientifique, Strasbourg, France.
Paulus
F
F
Otten
L
L
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015321:Gene Rearrangement; D005798:Genes, Bacterial / Q000235:genetics*; D007210:Indoleacetic Acids / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics; D015183:Restriction Mapping; D012230:Rhizobiaceae / Q000235:genetics; D012231:Rhizobium / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012764:Shigella sonnei / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D007210:Indoleacetic Acids; C081170:iaaM protein, Agrobacterium