Interleukin-10 stimulates rat pancreatic islets in vitro, but fails to protect against interleukin-1.
Rat pancreatic islets were cultured for 42 h in medium supplemented without or with 0.1-10 ng/ml of human interleukin-10 (IL-10). Medium insulin accumulation was increased by 50% after culture with 0.1 ng/ml IL-10. All concentrations of IL-10 increased the islet insulin content, but did not affect the islet DNA content. IL-10 did neither affect basal and glucose-stimulated insulin secretion nor (pro)insulin and total protein biosynthesis rates. Addition of 25 U/ml of interleukin-1 beta during the last 24 h of culture, led to a marked inhibition of glucose-stimulated insulin release and a 5-6 fold increase in nitrite production by rat insulinoma cells. These events could not be counteracted by any concentration of IL-10. The present data suggest that IL-10 might in some aspects stimulate pancreatic beta-cell function, but it fails to block the inhibitory action of IL-1 beta.
Department of Medical Cell Biology, Uppsala University, Sweden.
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S
Sandler
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Welsh
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004347:Drug Interactions; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma; D007375:Interleukin-1 / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D010190:Pancreatic Neoplasms; D011384:Proinsulin / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D014316:Tritium; D014407:Tumor Cells, Cultured
D007328:Insulin; D007375:Interleukin-1; D011994:Recombinant Proteins; D014316:Tritium; D016753:Interleukin-10; D004247:DNA; D011384:Proinsulin; D007930:Leucine; D005947:Glucose