The octamer binding protein Oct-2 inhibits transactivation of the herpes simplex virus immediate-early genes by the virion protein Vmw65.
Transactivation by a complex of the cellular transcription factor Oct-1 and the virion protein Vmw65 is necessary for the high-level activity of the HSV immediate-early promoters during lytic infection. We show that this trans-activation can be inhibited by two forms of the Oct-2 transcription factor which are expressed at high levels in neuronal cells as well as by the isolated DNA binding, POU domain of Oct-2. The inhibition of Oct-1-Vmw65 DNA binding by these neuronal forms of Oct-2 is likely to play a critical role in the nonpermissivity of neuronal cells for the HSV lytic cycle and therefore in the establishment of latent infections.
Lillycrop
K A
KA
Division of Molecular Pathology, University College London Medical School, United Kingdom.
Estridge
J K
JK
Latchman
D S
DS
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins; D005091:Exons / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics*; D017874:Immediate-Early Proteins; D008969:Molecular Sequence Data; D050813:Octamer Transcription Factor-2; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D018139:Simplexvirus / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014771:Virion / Q000235:genetics
D004268:DNA-Binding Proteins; D017405:Herpes Simplex Virus Protein Vmw65; C050678:IE1 protein, Human herpesvirus 1; D017874:Immediate-Early Proteins; D050813:Octamer Transcription Factor-2; C058435:POU2F2 protein, human; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D015500:Chloramphenicol O-Acetyltransferase