Protein and enzyme content of plasma membranes derived from Walker 256 carcinoma cells grown as ascitic or solid tumours.
A preliminary investigation of enzymes functioning at the cell surface indicates that when Walker 256 carcinoma cells growing ascitically are induced to grow as a solid tumour there is a marked increase in the activities of Na+, K(+)-ATPase, Mg(2+)-ATPase, 5'-nucleotidase, alkaline phosphatase and phosphodiesterase I; 5'-nucleotidase and alkaline phosphatase being particularly affected. These enzyme changes occur in the absence of any major alteration in the protein composition of the plasma membrane.
Clark
C M
CM
School of Biological and Medical Sciences, Division of Cell and Molecular Biology, University of St. Andrews, Fife, Scotland, UK.
Goodlad
G A
GA
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001202:Ascitic Fluid; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002279:Carcinoma 256, Walker / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D046508:Culture Techniques; D005260:Female; D008565:Membrane Proteins / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009368:Neoplasm Transplantation; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012878:Skin Neoplasms; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D008565:Membrane Proteins; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D009699:N-Glycosyl Hydrolases; C030681:purine nucleosidase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase