Modified purification of L-aromatic amino acid decarboxylase from pig kidney.
A new protocol for purification of L-aromatic amino acid decarboxylase from pig kidney in which, after an initial ultracentrifugation, only two columns are required to obtain homogeneous enzyme is presented. The protocol provides protein with a higher specific activity than that previously obtained, reducing by about one-half the time required for purification.
Dominici
P
P
Università Cattolica del Sacro Cuore, Facoltà di Medicina e Chirurgia A. Gemelli, Istituto di Chimica Biologica, Rome, Italy.
Moore
P S
PS
Voltattorni
C B
CB
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000737:chemistry / Q000302:isolation & purification*; D002847:Chromatography, Agarose; D007668:Kidney / Q000201:enzymology*; D008970:Molecular Weight; D011732:Pyridoxal Phosphate; D012685:Sepharose / Q000031:analogs & derivatives; D013552:Swine
D011732:Pyridoxal Phosphate; C020927:phenyl-sepharose; D012685:Sepharose; D001142:Aromatic-L-Amino-Acid Decarboxylases