Expression of synaptotagmin and syntaxin associated with N-type calcium channels in small cell lung cancer.
The presence of synaptic proteins involved in excitation/secretion coupling was examined in ten small cell lung cancer lines. N-Type calcium channels (omega-conotoxin receptors), synaptotagmin (p65) and syntaxin (HPC-1) were detected in eight. Co-immunoprecipitation experiments indicated that syntaxin can form a complex with synaptotagmin and calcium channels. The expression of synaptotagmin in small cell lung cancer may elicit an autoimmune response that reduces transmitter release at the nerve terminal.
INSERM U374, Faculté de Medecine Secteur Nord, Marseille, France.
P
P
David
O
O
el Far
N
N
Martin-Mouto
M F
MF
Poupon
M
M
Takahashi
M J
MJ
Seagar
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis / Q000378:metabolism*; D015153:Blotting, Western; D015220:Calcium Channels / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002793:Cholic Acids; D006801:Humans; D007163:Immunosorbent Techniques; D008175:Lung Neoplasms / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D050863:Synaptotagmin I; D050857:Synaptotagmins; D050827:Syntaxin 1; D014407:Tumor Cells, Cultured; D020866:omega-Conotoxin GVIA
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015220:Calcium Channels; D002135:Calcium-Binding Proteins; D002793:Cholic Acids; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D010455:Peptides; C494212:STX1A protein, human; C494213:Stx1a protein, rat; D050863:Synaptotagmin I; D050827:Syntaxin 1; D050857:Synaptotagmins; D020866:omega-Conotoxin GVIA; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate