The myosin molecule--charge response to nucleotide binding.
A decrease in the net fixed electric charge in the A-bands of cross-striated muscle was observed by Bartels and Elliott [2,10] when the muscle went from the rigor to the relaxed condition. The current work localises the source of the charge decrease by following the net charge on myosin (in the form of concentrated gels) and also myosin rod and light meromyosin gels when the gels are exposed to different concentrations of ATP. The work includes a study of muscle A-bands when the muscle is exposed to the same variations in ATP concentrations as the protein gels. The work shows that (i) Only 100-200 microns ATP is needed to initiate the charge decrease between the rigor and relaxed conditions; (ii) the effect of ATP is seen in the muscle A-band and the myosin and myosin rod gels, but not in LMM gels; (iii) pyrophosphate (PPi) shows a similar charge effect to ATP. ADP does not affect the charge on myosin gels, on the other hand. The results suggest that the charge decrease caused by ATP or PPi is due to ligand interaction with one or more sites on the myosin molecule. This interaction causes a disseminated effect in the protein, and a consequent loss in net negative charge either by a decrease in the absorption, of anions to Saroff sites on the protein, or, less probably, by an increase in the absorption of cations at those sites.
Bartels
E M
EM
Biophysics Group, Open University Oxford Research Unit, Boars Hill, UK.
Cooke
P H
PH
Elliott
G F
GF
Hughes
R A
RA
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D000838:Anions; D011756:Diphosphates / Q000494:pharmacology; D004563:Electrochemistry; D008024:Ligands; D015879:Myosin Subfragments / Q000494:pharmacology; D009218:Myosins / Q000737:chemistry / Q000378:metabolism*; D009994:Osmolar Concentration; D011817:Rabbits
D000838:Anions; D011756:Diphosphates; D008024:Ligands; D015879:Myosin Subfragments; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009218:Myosins