Detection of human papillomavirus type 2a DNA in verrucae vulgares by electron microscopic in situ hybridization.
Electron microscopic in situ hybridization (EMISH) of common warts (verrucae vulgares) of the hands was performed using a biotinylated human papillomavirus type 2a (HPV-2a) DNA probe and immunogold labelling of ultrathin sections of 2% glutaraldehyde-fixed, Lowicryl K4M-embedded tissues. It was first established that the warts contained HPV-2a DNA by light microscopic in situ hybridization. The HPV-2a probe chiefly labelled cells in the horny, granular and upper spinous layers of the epidermis, and labelling decreased towards the basal cell layer. The gold particles were located precisely on the viral particles in the nuclei of granular cells. The lower limit of detection by EMISH was found to be the keratinocytes of the third cellular layer above the basal cells. These keratinocytes showed evidence of a viral cytopathic effect, suggesting that vegetative DNA replication in infected keratinocytes occurs at least as early as this level of the epidermis.
Department of Dermatology, Faculty of Medicine, Tottori University, Yonago, Japan.
Y
Y
Hagari
M
M
Shibata
M
M
Mihara
S
S
Shimao
T
T
Kurimura
D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D017403:In Situ Hybridization; D008854:Microscopy, Electron; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D014860:Warts / Q000382:microbiology* / Q000473:pathology
D004279:DNA, Viral