Phospholipase A2 affects the activity of fusicoccin receptors.
Biochemical properties of fusicoccin receptors are strongly influenced by the phospholipid environment. In this report we have studied the effect of different exogenous phospholipases on fusicoccin binding ability of both plasma membrane and solubilised receptors. Among the phospholipases tested only phospholipase A2 showed an inhibitory effect on fusicoccin binding. In particular, the influence of this enzyme on the time course and reversibility of the fusicoccin binding reaction was studied. The inhibitory effect of phospholipase A2 was the consequence of fatty acid release. The usual fatty acids of plasma membrane phospholipids were active in inhibiting the interaction of fusicoccin with its receptors. It is concluded that a phospholipid associated to the fusicoccin receptor might play a significant role in the modulation of binding.
Aducci
P
P
Department of Biology, II University of Rome Tor Vergata, Italy.
Ballio
A
A
Donini
V
V
Fogliano
V
V
Fullone
M R
MR
Marra
M
M
D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology; D006027:Glycosides / Q000378:metabolism*; D008055:Lipids / Q000494:pharmacology; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010940:Plant Proteins; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism
D005227:Fatty Acids; D006027:Glycosides; D008055:Lipids; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C080425:fusicoccin receptor protein, plant; C007808:fusicoccin; D010741:Phospholipases A; D054467:Phospholipases A2