Myoinositol tris-phosphate-phytase complex as an elicitor in calcium mobilization in plants.
Mobilization of Ca2+ from microsomal/vacuolar fractions was detected when InsP6-phytase was added after a definite time of hydrolysis which coincides with the time (20-30 min) of optimal production of Ins(2,4,5)P3 bound to phytase. The in vitro constituted Ins(1,4,5)P3 or Ins(2,4,5)P3-phytase complex is also effective in releasing Ca2+. InsP3-phytase complex releases 45% more microsomal Ca2+ than that released by free InsP3 under identical conditions. Other inositol-phytase complexes are ineffective. Furthermore InsP3-phytase complex is recognised by putative receptor associated with microsomal fraction suggesting that the myoinositol tris-phosphate-phytase complex can act as an elicitor in Ca2+ mobilization in plant systems where phytate and phytase occur.
Department of Biochemistry, Bose Institute, Centenary Building, Calcutta, India.
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Samanta
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Dalal
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Biswas
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Biswas
D010832:6-Phytase / Q000378:metabolism*; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism*; D007887:Fabaceae / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism; D010946:Plants, Medicinal
D010833:Phytic Acid; D015544:Inositol 1,4,5-Trisphosphate; D010832:6-Phytase; D002118:Calcium