A gene encoding a highly expressed spindle body protein of Heliothis armigera entomopoxvirus.
The gene encoding the most abundant protein of purified preparations of Heliothis armigera entomopoxvirus (HaEPV) has been cloned and sequenced. The gene sequence encodes a 40.1K polypeptide with a putative N-terminal 20 amino acid leader peptide, and a single potential N-glycosylation site. Analysis of the protein, which has an apparent M(r) of 50K on polyacrylamide gels, confirmed post-translational loss of the leader peptide, but showed no evidence of glycosylation. The protein is related to others previously described from Choristoneura biennis EPV (63% identity) and Autographa californica nuclear polyhedrosis virus (42% identity). Polyclonal antiserum raised against a bacterial fusion protein containing the majority of the HaEPV protein specifically labelled HaEPV spindle bodies; confocal laser scanning microscopy suggests that the protein is distributed throughout those viral structures.
CSIRO Division of Entomology, Canberra, ACT, Australia.
D
D
Dall
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Sriskantha
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A
Vera
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J
Lai-Fook
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T
Symonds
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006031:Glycosylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009036:Moths / Q000382:microbiology*; D009838:Oligodeoxyribonucleotides; D011212:Poxviridae / Q000235:genetics* / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification
D009838:Oligodeoxyribonucleotides; D021382:Protein Sorting Signals; D014764:Viral Proteins; C083247:spindle body protein, Heliothis armigera entomopoxvirus