Fine analysis of recognition sites of monoclonal antibodies, 1B7 and 1E4, using L cell transfectants of recombinant mouse MHC class II genes.
Recognition sites of two monoclonal antibodies (mAbs), 1B7 and 1E4, specific for murine MHC class II were determined, using eleven L cell transfectants which express recombinant class II (I-A) molecules. 1B7 positive cells possessed a haplotype k in the alpha-helix of the beta-chain, whereas 1E4 positive cells had haplotype b at the alpha-helix of the beta-chain. The alpha-helix of the beta-chains is regarded as an important site for antigen binding. Thus, the 1B7 and 1E4 mAbs may be useful for analysis of functional sites on class II molecules involved in antigen recognition.
Itoh
Y
Y
Section of Pathology, Hokkaido University, Sapporo, Japan.
Ogasawara
K
K
Onoé
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D005802:Genes, MHC Class II; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000235:genetics; D007739:L Cells; D051379:Mice; D008815:Mice, Inbred Strains; D017433:Protein Structure, Secondary; D014162:Transfection
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II