Hyaluronan can be protected from free-radical depolymerisation by 2,6-diisopropylphenol, a novel radical scavenger.
The scavenging effect of 2,6-diisopropylphenol on hydroxy radicals produced by xanthine oxidase was assessed by evaluating the in vitro depolymerisation of hyaluronan in artificial synovial fluid by size-exclusion chromatography. After 1 hour, the number-average molecular weight of hyaluronan remained unchanged (100%) with 2,6-diisopropylphenol, whereas it dropped to 90% with methylprednisolone added, to 55% with the antioxidant 2,6-tert-butyl-4-methylphenol added, and to 10% of its initial value in the absence of scavenger.
POLY-biòs Research Centre, LBT-Area di Ricerca, Trieste, Italy.
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Kvam
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Granese
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Flaibani
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Pollesello
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Paoletti
D002850:Chromatography, Gel; D016166:Free Radical Scavengers; D005609:Free Radicals; D006820:Hyaluronic Acid / Q000737:chemistry*; D006878:Hydroxides; D017665:Hydroxyl Radical; D007700:Kinetics; D046911:Macromolecular Substances; D015742:Propofol; D013997:Time Factors
D016166:Free Radical Scavengers; D005609:Free Radicals; D006878:Hydroxides; D046911:Macromolecular Substances; D017665:Hydroxyl Radical; D006820:Hyaluronic Acid; D015742:Propofol