Expression of calcium-binding proteins MRP8 and MRP14 is associated with distinct monocytic differentiation pathways in HL-60 cells.
MRP8 and MRP14 are two calcium-binding proteins of the S-100 family which are expressed during distinct stages of monocytic maturation. To further investigate their regulation the human leukemic cell line HL-60 which can be induced to differentiate to monocytes/macrophages by 12-O-tetradecanoylphorbol 13-acetate (TPA), 1,25-(OH)2 Vitamin D3 (VD3), tumor necrosis factor-alpha (TNF-alpha) and interferon-g (IFN-g) were analyzed for expression of MRP8/MRP14. Employing Northern blotting, a sandwich enzyme-linked immunosorbent assay and immunocytochemical analysis we determined MRP8/MRP14 mRNA and protein levels, which were found to be elevated after VD3 and reduced after TPA treatment. TNF-alpha and IFN-g did not affect MRP8/MRP14 levels. Western blot analysis revealed that formation of MRP8/MRP14 to biologically active complexes which has previously been shown to be a calcium-mediated process is not dependent on the differentiation stages of HL-60 cells. Restriction of MRP8/MRP14 expression to only distinct pathways of monocytic differentiation in HL-60 cells may thus reflect different functional phenotypes of monocytes/macrophages in vivo.
Institute of Experimental Dermatology, University of Münster, Germany.
J
J
Roth
M
M
Goebeler
C
C
van den Bos
C
C
Sorg
D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D040501:Calgranulin A; D040502:Calgranulin B; D002454:Cell Differentiation; D002762:Cholecalciferol / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002762:Cholecalciferol; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate