Analysis of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus trans-activators IE-1 and IE-2 using monoclonal antibodies.
We have produced monoclonal antibodies against two Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) transcriptional trans-activators, IE-1 and IE-2. Temporal analysis of IE-1 and IE-2 proteins have shown that IE-1 continues to increase in steady-state levels from 0 to 120 h post-infection, whereas IE-2 declines by 36 h post-infection. At least five different electrophoretic forms of IE-1 are present in OpMNPV-infected LD652Y cells of which three appear to be from the non-spliced IE-1 gene. Cotransfection experiments also showed that IE-1 causes a reduction in the levels of IE-2 protein but concurrently IE-2 increases IE-1 expression. Western blot analysis indicated that a form of IE-1 copurified with budded virions but did not copurify with the polyhedron-derived virus.
Agriculture Canada Research Station, Vancouver, British Columbia.
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Theilmann
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Stewart
D000818:Animals; D000911:Antibodies, Monoclonal; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D003360:Cosmids; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral; D017874:Immediate-Early Proteins; D009036:Moths; D015534:Trans-Activators / Q000032:analysis / Q000096:biosynthesis*; D014162:Transfection; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
C069183:Ac-IE-1 protein, Autographa californica nucleopolyhedrovirus; D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D015534:Trans-Activators; D014764:Viral Proteins; C072773:IE2 protein, polyhedrosis virus