Towards covering immunological genes with highly informative markers: a trans-species approach.
To establish a highly informative screening system for immunologically relevant genes ("immunoprinting") we co-amplified via polymerase chain reaction (PCR) polymorphic exons plus adjacent intronic simple repetitive dinucleotide stretches in the T-cell receptor (Tcr) Vb6 and Major Histocompatibility Complex (MHC)-DRB loci in man and several ungulate species. In both gene families the basic structure of the simple repeat was found to be preserved for more than 70 x 10(6) years in all investigated species. The simple repeats exhibit extensive length variability. Distinct exon sequences are correlated with a defined repeat length and substructure. In addition, PCR and the oligonucleotides for typing were applicable to a broad range of species from different mammalian orders. Multiplex PCR of different members of the Tcr Vb6 family and MHC-DRB resulted in a complex pattern similar to an oligolocus fingerprint. Hence immunoprinting can be employed for searching for associations of immunologically relevant genes with diseases even across species barriers.
Ruhr-University, Bochum, Germany.
J
J
Buitkamp
W
W
Schwaiger
C
C
Epplen
M
M
Gomolka
E
E
Weyers
J T
JT
Epplen
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting; D005260:Female; D005802:Genes, MHC Class II; D005819:Genetic Markers; D006041:Goats; D015808:HLA-DR6 Antigen / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012756:Sheep; D013045:Species Specificity
D005819:Genetic Markers; D015808:HLA-DR6 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA