Normal and aberrant expression of cytokines in neoplastic cells from chronic lymphocytic leukemias.
Molecular expression of cytokines and cytokine receptors associated with B-cell growth and differentiation was examined in cells from B-cell chronic lymphocytic leukemia patients using the PCR. These studies were undertaken in order to determine whether a particular cytokine could be associated with leukemic transformation in this disease. The precursor-lymphoid and pro-B, pre-B-cell growth factor, interleukin-7, was found to be expressed in 30 of 30 patients, whereas, it was not expressed in normal donor peripheral blood lymphocytes (0 of 8) or in purified B-cell subsets from normal individuals. IL-1 beta and IL-2 receptors, on the other hand, were expressed by B cells from both normal and B-CLL patients. Other cytokine and cytokine receptors examined were not consistently expressed by all donors. Thus IL-7 was found to be the only cytokine tested that was expressed in CLL cells and not in normal cells.
Section of Medical Oncology, Rush-Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612.
J M
JM
Plate
W H
WH
Knospe
J E
JE
Harris
S A
SA
Gregory
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000943:Antigens, Differentiation / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000378:metabolism; D001483:Base Sequence; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism
D000943:Antigens, Differentiation; D016207:Cytokines; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic