Delaminomycins, novel nonpeptide extracellular matrix receptor antagonist and a new class of potent immunomodulator. I. Taxonomy, fermentation, isolation and biological activity.
In order to develop a new class of immunomodulator we have searched for a low molecular weight inhibitors of cell adhesion to components of extracellular matrix (ECM), fibronectin (FN), laminin (LM) and collagen type IV (CL), and succeeded to find a group of novel antibiotics, named delaminomycins. Delaminomycins were isolated from the mycelium and cultured broth of Streptomyces albulus MJ202-72F3. It was purified by use of centrifugal partition chromatography (CPC), preparative reverse phase HPLC and Sephadex LH-20 and was obtained as a white powder. Delaminomycins with inhibitory activity for cell adhesion to ECM components suppressed immune responses in vitro and in vivo and exhibited antimicrobial activity on Gram-positive bacteria.
Institute for Chemotherapy, Microbial Chemistry Research Foundation, Shizuoka, Japan.
M
M
Ueno
M
M
Amemiya
M
M
Iijima
M
M
Osono
T
T
Masuda
N
N
Kinoshita
T
T
Ikeda
H
H
Iinuma
M
M
Hamada
M
M
Ishizuka
D000276:Adjuvants, Immunologic / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification* / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000037:antagonists & inhibitors; D005285:Fermentation; D005353:Fibronectins / Q000037:antagonists & inhibitors; D007797:Laminin / Q000037:antagonists & inhibitors; D008546:Melanoma, Experimental / Q000188:drug therapy; D051379:Mice; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D051381:Rats; D011916:Rats, Inbred F344; D011921:Rats, Inbred WKY; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors*; D013302:Streptomyces / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D000903:Antibiotics, Antineoplastic; D005353:Fibronectins; D007797:Laminin; D011956:Receptors, Cell Surface; C052131:extracellular matrix receptor; D003094:Collagen