Biological activities of surface-associated material from Porphyromonas gingivalis.
Surface-associated material (SAM) from Porphyromonas gingivalis was tested for in vitro biological activities that may be relevant to the pathogenesis of chronic periodontitis. SAM was found to stimulate bone resorption at a concentration of 1.0 microgram/ml and this was inhibited by indomethacin, interleukin-1 receptor antagonist protein and anti-tumour necrosis factor antibody. At a concentration of 10 ng/ml, the SAM inhibited DNA and collagen synthesis in osteoblasts and murine calvaria and DNA synthesis in fibroblasts, monocytes and epidermal cells. Therefore, easily solubilised surface components from P. gingivalis could play a role in the pathogenesis of chronic periodontitis if these activities operate in vivo.
Wilson
M
M
Microbiology Laboratory, Institute of Dental Surgery, London, UK.
Meghji
S
S
Barber
P
P
Henderson
B
B
D000818:Animals; D001442:Bacteroides Infections / Q000382:microbiology / Q000503:physiopathology; D001862:Bone Resorption; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D051379:Mice; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000187:drug effects; D010518:Periodontitis / Q000382:microbiology / Q000503:physiopathology; D016966:Porphyromonas gingivalis / Q000737:chemistry* / Q000472:pathogenicity / Q000648:ultrastructure; D012886:Skull / Q000166:cytology; D014774:Virulence
D003094:Collagen; D004247:DNA