Superoxide radical scavenging activity of phenolic compounds.
1. Superoxide radicals (O2-.) were generated from a hypoxanthin-xanthin oxidase (HPX-XOD) reaction system and detected by the electron spin resonance (ESR) spin-trapping technique. 2. The inhibitory effect on O2-. generation occurred in the order: superoxide dismutase (SOD) > L-ascorbic acid > eugenol > guaiacol > phenol. 3. And the second-order rate constants between O2-. and samples were: SOD 1.7 x 10(9)/M/S, L-ascorbic acid 3.4 x 10(5)/M/S, eugenol 8.3 x 10(3)/M/S, guaiacol 2.5 x 10(3)/M/S and phenol 5.8 x 10(2)/M/S. 4. It was clarified that the phenolic compounds have O2-. scavenging activity.
Department of Endodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Y
Y
Tsujimoto
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H
Hashizume
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M
Yamazaki
D001205:Ascorbic Acid / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005054:Eugenol / Q000494:pharmacology; D016166:Free Radical Scavengers; D006139:Guaiacol / Q000494:pharmacology; D007700:Kinetics; D010636:Phenols / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D010636:Phenols; D013481:Superoxides; D005054:Eugenol; D006139:Guaiacol; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D001205:Ascorbic Acid