Platelet aggregation induced by calceolarioside A in vitro: role of platelet intracellular calcium.
The effect of calceolarioside A, a phenylpropanoid glycoside (PhG), isolated from Calceolaria hypericina, was studied on rabbit platelets in vitro. Calceolarioside A induced a dose-related aggregant effect on rabbit platelets. Indomethacin did not modify the calceolarioside A-induced aggregant effect. Furthermore, no modification was exerted by phenoxybenzamine, BW 577C, and WEB 2086 on the PhG aggregant effect. On the contrary, TMB-8, an intracellular calcium blocker, significantly reduced the PhG effect. The latter result suggests that calceolarioside A aggregation may be partly related to a calcium-dependent mechanism.
Dipartimento di Farmacologia Sperimentale, Università degli Studi di Napoli Federico II, Italy.
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D000818:Animals; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D015394:Molecular Structure; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits
D002109:Caffeic Acids; D005960:Glucosides; D006027:Glycosides; C083066:calceolarioside A; D002118:Calcium