Simultaneous high-performance liquid chromatographic determination of lornoxicam and its 5'-hydroxy metabolite in human plasma using electrochemical detection.
A selective and sensitive high-performance liquid chromatographic method with coulometric detection is described for the simultaneous quantitation of lornoxicam, a new oxicam class drug, and its 5'-hydroxy metabolite in human plasma. The two analytes and the internal standard are extracted from plasma at pH 4.0 by liquid-liquid extraction and separated on a C18 column. Absolute detection limits using 100 microliters of plasma are found to be 5 and 10 ng/ml for lornoxicam and 5'-hydroxylornoxicam, respectively. The assay described has been successfully applied to samples from clinical studies.
Department of Drug Metabolism, Research Center Taisho Pharmaceutical Co., Ltd., Saitama, Japan.
T
T
Suwa
H
H
Urano
Y
Y
Shinohara
J
J
Kokatsu
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D015203:Reproducibility of Results
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010894:Piroxicam; C059451:lornoxicam