Incorporation of [3H]inositol into phosphoinositides and inositol phosphates by rabbit blastocysts.
Preimplantation rabbit embryos collected at the early morula stage were cultured to blastocysts in the presence of [3H]inositol. The blastocysts were lysed, and both the aqueous and lipid portions were analysed for incorporated radioactivity. Thin-layer chromatographic separation of the lipid portion indicated that [3H]inositol was incorporated into phosphatidylinositol, phosphatidylinositol 4-phosphate, and phosphatidylinositol 4,5-bisphosphate. HPLC anion-exchange chromatography indicated that [3H]inositol was incorporated into inositol phosphates, including the two second messengers, inositol 1,4,5-triphosphate and inositol 1,3,4,5-tetrakisphosphate, and also inositol monophosphate and inositol 1,4-bisphosphate. These results provide evidence that rabbit blastocysts may have an active phosphatidylinositol second messenger system, which may be responsive to intrauterine factors or intraembryonic paracrine factors.
Department of Physiology, University College, Galway, Ireland.
M M
MM
Fahy
M T
MT
Kane
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D066298:In Vitro Techniques; D007294:Inositol / Q000378:metabolism*; D007295:Inositol Phosphates / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000096:biosynthesis; D011817:Rabbits; D015290:Second Messenger Systems / Q000502:physiology
D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D007294:Inositol