Specific induction of a functional endogenous D2 short dopamine receptor in GH4C1 cells.
In contrast to lactotrophs, tumoral pituitary cells like GH3 and GH4C1 lack expression of dopamine D2short and D2long receptors. In GH4C1 cells, we observed that the expression of only the short isoform of D2 receptor can be induced after transfection with a plasmid which confers resistance to neomycin (pRSVNeo). High levels of fully functional D2short receptor were obtained in GH4C1 following transfection (528fmol/mg protein). Sequence, pharmacology and coupling of the induced-D2 receptor do not show any difference with the cloned rat D2 short receptor.
Dept. of Ontogénèse et Génétique Moléculaire, CHUL, Ste-Foy, Qc, Canada.
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Allard
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Lapointe
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Falardeau
D000818:Animals; D001058:Apomorphine / Q000031:analogs & derivatives / Q000494:pharmacology; D001483:Base Sequence; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004873:Ergolines / Q000494:pharmacology; D015870:Gene Expression; D019868:Kanamycin Kinase; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010770:Phosphotransferases / Q000096:biosynthesis / Q000235:genetics; D010911:Pituitary Neoplasms; D016133:Polymerase Chain Reaction; D011189:Potassium Chloride / Q000494:pharmacology; D011388:Prolactin / Q000378:metabolism; D019257:Quinpirole; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D013134:Spiperone / Q000378:metabolism; D013469:Sulpiride / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D004873:Ergolines; D009838:Oligodeoxyribonucleotides; D017448:Receptors, Dopamine D2; D011993:Recombinant Fusion Proteins; D005576:Colforsin; D019257:Quinpirole; D014660:Vasoactive Intestinal Peptide; D013134:Spiperone; C009074:N-n-propylnorapomorphine; D011189:Potassium Chloride; D013469:Sulpiride; D011388:Prolactin; D000242:Cyclic AMP; D010770:Phosphotransferases; D019868:Kanamycin Kinase; D001058:Apomorphine; D004298:Dopamine