Interleukin 7 receptor mediates the activation of phosphatidylinositol-3 kinase in human B-cell precursors.
Interleukin 7 appears to be central to promoting growth and differentiation of lymphocyte precursors. Pro-B cells, as well as pre-pre-B cells, but not mature cells express the IL-7 receptor suggesting an important role for IL-7 during these early stages of B cell maturation. Indeed, IL-7 was shown to promote not only growth but also immunoglobulin gene rearrangement in these cells. However, the mechanism by which IL-7 receptor transduces these growth signals remains elusive. In an attempt to characterize a pathway of signal transduction via the IL-7 receptor, we have demonstrated here that ligation of the IL-7 receptor is associated with increased activity of PI-3 kinase. PI-3 kinase products were detected by TLC and their identity confirmed by HPLC. Further, by in vivo labeling intact cells we have shown that IL-7 receptor mediates the activation of PI-3 kinase.
Division of Immunology/Allergy, Hospital for Sick Children, Toronto, ON.
H K
HK
Dadi
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C M
CM
Roifman
D001402:B-Lymphocytes / Q000166:cytology / Q000201:enzymology*; D004789:Enzyme Activation; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D015851:Interleukin-7 / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases; D010770:Phosphotransferases / Q000378:metabolism*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood; D011971:Receptors, Immunologic / Q000378:metabolism*; D020395:Receptors, Interleukin-7; D014407:Tumor Cells, Cultured
D015851:Interleukin-7; D011971:Receptors, Immunologic; D020395:Receptors, Interleukin-7; D010770:Phosphotransferases; D019869:Phosphatidylinositol 3-Kinases