Comparison of RAG gene expression in normal and transformed precursor lymphocytes.
Analyses of mechanisms that regulate V(D)J recombination have relied heavily on the use of transformed precursor lymphocyte cell lines. We now show that such lines have highly variable and frequently low levels of recombination activating genes (RAG)-1 and -2 gene expression. We also show that expression levels of the RAG genes can vary > 100-fold between different subcloned cells of an individual pre-B line. We discuss these findings in the context of normal regulation of RAG gene expression and the implication for the use of transformed pre-B cell lines as models for studying control of V(D)J recombination activity.
Rathbun
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Howard Hughes Medical Institute, Boston, MA.
Oltz
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D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004268:DNA-Binding Proteins; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D016654:Genes, RAG-1; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D018398:Homeodomain Proteins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000235:genetics*; D011995:Recombination, Genetic
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D011506:Proteins; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein