Spreading the synaptonemal complex of Neurospora crassa.
A protocol was developed to spread the synaptonemal complex (SC) of the fungus Neurospora crassa. It involves direct mechanical breakage of meiotic cells before spreading. This technique makes it possible to examine the SC of the same nucleus with both light and electron microscopy. This protocol is potentially applicable for other Pyrenomycetes. The SCs were examined at zygotene, pachytene and diplotene. The central elements and the recombination nodules (RN) were well revealed by silver staining. Ten to 13 RNs were counted at pachytene. The total genomic SC length varied with the stage. This whole mount electron microscopy of the SC is particularly useful for studying chromosomal rearrangements.
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Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
D016681:Genome, Fungal; D008540:Meiosis; D008854:Microscopy, Electron; D009172:Mycology / Q000379:methods; D009492:Neurospora crassa / Q000235:genetics / Q000648:ultrastructure*; D011995:Recombination, Genetic; D013573:Synaptonemal Complex / Q000235:genetics; D014178:Translocation, Genetic