Development of a flow system for the microscopic observation of cultured nerve cells and its application to the neurite growth control of PC12h-R.
A flow system has been developed for the continual microscopic observation of cultured nerve cells. The undefined effects of coexisting cells could be efficiently reduced by the continuous flow of a medium. The present flow system was useful also for the experiments requiring medium exchanges. Its performance was demonstrated by the repeated actions of NGF or db-cAMP to PC12h-R cells. The neurite projection and its retraction were repeatedly observed by the supply and the removal of those factors.
Kazuno
Y
Y
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Homma
T
T
Nemoto
Y
Y
Matsuoka
H
H
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002452:Cell Count; D003470:Culture Media; D005434:Flow Cytometry / Q000295:instrumentation*; D009414:Nerve Growth Factors / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000166:cytology*; D016716:PC12 Cells / Q000166:cytology
D003470:Culture Media; D009414:Nerve Growth Factors; D003994:Bucladesine