Localization of pig Na+,K(+)-ATPase alpha and beta subunit genes to chromosome 4 by radioactive in situ hybridization.
Two genes coding for Na+,K(+)-ATPase alpha and beta subunits are localized on pig chromosome 4, to the q1.6-->q2.3 and 1.3-->q2.1 regions, respectively, by radioactive in situ hybridization. According to nucleotide and amino acid sequence comparisons with different human isoforms of Na+,K(+)-ATPase, these pig alpha beta ATPase genes show strong homologies with human alpha 1 and beta subunit ATPase genes, respectively. These results are discussed with respect to comparative mapping data of conserved genes in mammalian species. We showed that the pig cDNA probes encoding ATPase alpha and beta genes reveal DNA polymorphism in Meishan and Large White pigs.
INRA, Centre de Recherches de Toulouse, Castanet-Tolosan, France.
Y
Y
Lahbib-Mansais
M
M
Yerle
M
M
Dalens
C
C
Chevalet
J
J
Gellin
D000818:Animals; D002478:Cells, Cultured; D002874:Chromosome Mapping / Q000662:veterinary*; D006801:Humans; D017403:In Situ Hybridization / Q000662:veterinary; D007621:Karyotyping / Q000662:veterinary; D008677:Metaphase; D051379:Mice; D011110:Polymorphism, Genetic; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D000254:Sodium-Potassium-Exchanging ATPase