Efficient analysis of nonviable poliovirus capsid mutants.
Nonviable poliovirus capsid mutants were studied by an efficient infection-transfection system. Phenotypically, nonviable poliovirus capsid mutants appear to segregate into three classes: those that form only protomers, those that can form pentamers, and one that can form completed virions.
Simons
J
J
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139.
Rogove
A
A
Moscufo
N
N
Reynolds
C
C
Chow
M
M
D002213:Capsid / Q000032:analysis / Q000235:genetics*; D036022:Capsid Proteins; D006367:HeLa Cells; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D009500:Neutralization Tests; D017955:Poliovirus / Q000235:genetics* / Q000254:growth & development; D011499:Protein Processing, Post-Translational; D014162:Transfection / Q000379:methods*
D036022:Capsid Proteins; D009227:Myristic Acids; C442539:VP0 protein, Poliovirus; C442540:VP1 protein, Poliovirus; C442541:VP2 protein, Poliovirus; C442542:VP3 protein, Poliovirus; D019814:Myristic Acid