[Augmentation of cytotoxicity of nonparenchymal cell of the liver after continuous IL-2 administration via the portal vein].
In this study, we discussed the influence of recombinant interleukin-2 (rIL-2) on nonparenchymal cell of the liver. Donryu rats (8-10 weeks, male) were continuously administered rIL-2 (IL-2 group) or saline (control group) for 6 days via the portal vein. On day 0 or 7 after administration, rats were sacrificed to obtain the blood and liver. Blood mononuclear cell (BMC) and nonparenchymal cell (NPC) of the liver were collected, and their cytotoxicity (NK activity and LAK activity) was measured by 51Cr release-assay. NK activity and LAK activity of BMC in IL-2 group were increased on day 0 and day 7 compared to those in the control group. NK activity and LAK activity of NPC in IL-2 group were augmented on day 0, and NK activity in IL-2 group was still high on day 7. These results suggest that preoperative IL-2 administration may be effective for prevention of formation and/or proliferation of liver metastasis, that are provoked by surgical intervention in cancer of the digestive organs.
Second Dept. of Surgery, Yamaguchi University School of Medicine.
K
K
Shimoda
M
M
Oka
S
S
Hazama
M
M
Suzuki
N
N
Lizuka
K
K
Wadamori
F
F
Wang
T
T
Suzuki
D000818:Animals; D003602:Cytotoxicity, Immunologic; D015918:Infusion Pumps, Implantable; D007376:Interleukin-2 / Q000008:administration & dosage*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D007958:Leukocyte Count; D008099:Liver / Q000166:cytology / Q000276:immunology*; D008297:Male; D011169:Portal Vein; D051381:Rats; D011994:Recombinant Proteins / Q000008:administration & dosage
D007376:Interleukin-2; D011994:Recombinant Proteins