The changes of prooxidant and antioxidant enzyme activities in bovine leukemia virus-transformed cells. Their influence on quinone cytotoxicity.
It was found that the activities of prooxidant enzymes (NAD(P)H oxidases and NAD(P)H:cytochrome c reductases) in bovine leukemia virus-transformed calf and lamb embryo kidney fibroblasts (lines Mi-18 and FLK) were by 1.25-18 times higher when compared to corresponding nontransformed calf cells. The activity of DT-diaphorase was also increased by about one order of magnitude in transformed cells. The activities of antioxidant enzymes were almost unchanged (superoxide dismutase), decreased by 13% or 53% (catalase) or increased by 25% or 90% (glutathione reductase) in Mi-18 or FLK cells, respectively. These changes of enzyme activity increased the toxicity of simple redox-cycling quinones (duroquinone, naphthazarin) towards transformed cells, but did not affect the toxicity of daunorubicin. The latter was most probably related to the inhibition of plasma membrane NADH dehydrogenase.
Institute of Immunology, Lithuanian Academy of Sciences, Vilnius.
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D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology; D002417:Cattle; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D002472:Cell Transformation, Viral; D004622:Embryo, Mammalian; D005292:Ferricyanides / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D007668:Kidney; D001909:Leukemia Virus, Bovine; D009097:Multienzyme Complexes / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D019255:NADPH Oxidases; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009285:Naphthoquinones / Q000494:pharmacology; D010084:Oxidation-Reduction; D011809:Quinones / Q000494:pharmacology*; D012756:Sheep
D016227:Benzoquinones; D005292:Ferricyanides; D009097:Multienzyme Complexes; D009285:Naphthoquinones; D011809:Quinones; C007931:hexacyanoferrate III; C005503:naphthazarin; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D009251:NADPH-Ferrihemoprotein Reductase; D019255:NADPH Oxidases; D016660:NAD(P)H Dehydrogenase (Quinone); D009245:NADH Dehydrogenase; C034257:duroquinone