Kinetic and antigenic characterization of altered protein synthesis by Campylobacter jejuni during cultivation with human epithelial cells.
Cultivation of Campylobacter jejuni with INT 407 cell monolayer cultures results in new or enhanced synthesis of a number of proteins compared with bacteria cultured in the absence of the epithelial cells. These proteins were detected within 60 min after the addition of the bacteria to the epithelial cell cultures, and their synthesis was temporally associated with an increase in C. jejuni internalization. A rabbit antiserum raised against bacteria that were cultivated with INT 407 cells recognized nine proteins that were not recognized by an antiserum against C. jejuni cultivated in medium alone. The former antiserum inhibited the internalization, but not the binding, of Campylobacter jejuni in a dose-dependent fashion. The results suggest that one or more of the proteins synthesized by C. jejuni in response to cocultivation with epithelial cells plays a role in facilitating internalization.
Konkel
M E
ME
Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
Mead
D J
DJ
Cieplak
W
W
D000942:Antigens, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D001345:Autoradiography; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis*; D016123:Campylobacter jejuni / Q000254:growth & development / Q000502:physiology*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D013462:Sulfur Radioisotopes; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine