The effect of aging on the genetic expression of renin by mouse kidney.
The effect of aging on the genetic expression of renin was studied in kidney tissue by measuring renin mRNA levels. RNA was isolated from the kidneys of 5- to 37-month-old male C57BL/6J mice. The relative levels of the renin mRNA were measured by dot blot hybridization using a cDNA probe to renin. The size of renin mRNA as determined by northern blot analysis was found to be 1.6 Kb. The size of renin mRNA did not change with increasing age. However, the levels of renin mRNA in kidney RNA decreased 70% after 12 months of age.
Hung
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Geriatric Research, Education, and Clinical Center, Audie L. Murphy Memorial Veterans Hospital, San Antonio, TX 78284.
Richardson
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A
D000375:Aging / Q000378:metabolism*; D000818:Animals; D015870:Gene Expression; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D012083:Renin / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*
D012333:RNA, Messenger; D012083:Renin