Soluble tumor necrosis factor receptors in human inflammatory synovial fluids.
OBJECTIVE
To test synovial fluid (SF) for the presence of soluble fragments originating from distinct tumor necrosis factor receptors (TNF-sR55 and TNF-sR75) which bind to TNF and inhibit its biologic activity.


METHODS
TNF-sR55 and TNF-sR75 were measured in 62 SF samples by specific immunoassays using monoclonal antibodies.


RESULTS
Both TNF-sR were present in all of the SF tested. Their concentrations were higher in SF from patients with seropositive rheumatoid arthritis than in patients with other inflammatory arthritides. The relative amount of TNF-sR75, as compared with TNF-sR55, was higher in seropositive RA SF than in other SF.


CONCLUSIONS
The balance between TNF and its specific inhibitors may be critical to the biologic outcome mediated by this cytokine.
Division of Immunology, University Hospital, Geneva, Switzerland.
P
P
Roux-Lombard
L
L
Punzi
F
F
Hasler
S
S
Bas
S
S
Todesco
H
H
Gallati
P A
PA
Guerne
J M
JM
Dayer
D006801:Humans; D007249:Inflammation / Q000473:pathology*; D007592:Joint Diseases / Q000473:pathology*; D009504:Neutrophils / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000032:analysis*; D018124:Receptors, Tumor Necrosis Factor; D012995:Solubility; D013582:Synovial Fluid / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha