Plasmid pSH: a strong mammalian expression vector.
Plasmid pSH is an expression vector enabling high level expression of inserted genes in a variety of cell types compared to SV40 early promoter. Here, constructs made with pSH have been tested in human primary or immortalized keratinocytes, primary human foreskin fibroblasts and mouse fibroblasts.
Ventura
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AM
Ludwig Institute for Cancer Research, Sao Paulo, Brazil.
Villa
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LL
D016475:3T3 Cells; D000818:Animals; D004268:DNA-Binding Proteins; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000378:metabolism; D010957:Plasmids; D014162:Transfection; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral