Lung protein leakage in feline septic shock.
The aim of the present study was to explore lung microvascular leakage of protein and water in a feline model of septic shock, using a double isotope technique with external gamma camera detection and gravimetric lung water measurements. The experiments were performed on artificially ventilated cats. One group of cats (n = 8) was given an infusion of live Escherichia coli bacteria, and another group (n = 5) served as a control group receiving saline. Plasma transferrin was radiolabeled in vivo with indium-113m-chloride, and erythrocytes were labeled with technetium-99m. The distribution of these isotopes in the lungs was continuously measured with a gamma camera. A normalized slope index (NSI) was calculated, indicative of the transferrin accumulation corrected for changes in local blood volume that reflect protein leakage. In the septic group there was a protein leakage after bacterial infusion, with a NSI of 39 x 10(-4) +/- 5 x 10(-4) min-1 (mean +/- SEM), and the PaO2 diminished from 21 +/- 1 to 9.5 +/- 1 kPa. In control cats a slight protein leakage with a NSI of 9 +/- 10(-4) +/- 2 x 10(-4) min-1 was detected, probably caused by the operative procedure, but PaO2 did not change. Wet-to-dry-weight ratios of postmortem lungs were not significantly different between the groups. It was concluded that an intravenous infusion of live E. coli bacteria induces a lung capillary protein leakage without increased lung water and a concomitantly disturbed gas exchange.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Pulmonary Medicine, Renstömska Hospital, Göteborg, Sweden.
K M
KM
Schützer
A
A
Larsson
B
B
Risberg
A
A
Falk
D000818:Animals; D002415:Cats; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000000981:diagnostic imaging / Q000378:metabolism*; D015633:Extravascular Lung Water / Q000737:chemistry / Q000000981:diagnostic imaging / Q000378:metabolism; D015902:Gamma Cameras; D007204:Indium; D007205:Indium Radioisotopes; D008168:Lung / Q000098:blood supply / Q000000981:diagnostic imaging / Q000378:metabolism*; D008833:Microcirculation / Q000000981:diagnostic imaging / Q000378:metabolism; D009929:Organ Size; D011506:Proteins / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D011877:Radionuclide Imaging; D012772:Shock, Septic / Q000000981:diagnostic imaging / Q000378:metabolism*; D013670:Sodium Pertechnetate Tc 99m; D014168:Transferrin / Q000378:metabolism
D007205:Indium Radioisotopes; D011506:Proteins; D014168:Transferrin; D007204:Indium; C020758:indium trichloride; D013670:Sodium Pertechnetate Tc 99m