Eclosion hormone activates phosphatidylinositol hydrolysis in silkworm abdominal ganglia during adult metamorphosis.
Eclosion hormone (EH), an insect neuropeotide, stimulated phosphatidylinositol (PtdIns) hydrolysis in abdominal ganglia isolated from Bombyx mori in a specific stage of adult development. Incubation of abdominal ganglia from silkworm pharate adults with EH led to an increase in formation of inositol 1,4,5-trisphosphate but this increase took place transiently, maximum increase being observed 30 s after the addition of EH. PtdIns hydrolysis was stimulated by exogenous EH in a dose-dependent fashion and was completely abolished by the phospholipase C inhibitors, neomycin and compound 48/80. The EH-induced PtdIns hydrolysis developed in parallel to the EH-induced eclosion behaviour during development of the adult. These results suggest that the EH-stimulated PtdIns hydrolysis plays an important role in EH-mediated signal transduction during adult development of B. mori.
Shibanaka
Y
Y
International Research Laboratories, Ciba-Geigy Ltd, Takarazuka, Japan.
Hayashi
H
H
Takai
M
M
Fujita
N
N
D000005:Abdomen; D000818:Animals; D012831:Bombyx / Q000254:growth & development* / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D005724:Ganglia / Q000187:drug effects / Q000378:metabolism*; D006868:Hydrolysis; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007301:Insect Hormones / Q000494:pharmacology*; D007700:Kinetics; D008675:Metamorphosis, Biological / Q000502:physiology*; D009355:Neomycin / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D007301:Insect Hormones; D010716:Phosphatidylinositols; D003189:p-Methoxy-N-methylphenethylamine; D015544:Inositol 1,4,5-Trisphosphate; C042645:eclosion hormone; D010738:Type C Phospholipases; D006152:Cyclic GMP; D009355:Neomycin