Chromosomal localization and nucleoside diphosphate kinase activity of human metastasis-suppressor genes NM23-1 and NM23-2.
Human metastasis-suppressor genes nm23-1 (NME1) and nm23-2 (NME2) are implicated in control of the metastatic potential of malignant cells. Using somatic cell hybrid analysis and fluorescence in situ hybridization we co-localized both genes to 17q21.3. The 17q21 region carries the locus responsible for early-onset familial breast-ovarian cancer and several other genes that are involved in tumorigenesis and differentiation and undergo frequent rearrangements during neoplastic development. Thus, our mapping places the NME genes in a region that may be subjected to multiple selection pressures. NME1 and NME2 genes were expressed as soluble proteins in a T7 bacterial expression system. Both proteins are independently active nucleotide diphosphate kinases and readily form intra- and intermolecular disulfide bonds. The biochemical properties of these proteins may explain the diversity of mature eucaryotic nucleoside diphosphate kinases.
Backer
J M
JM
Molecular Biology Research Section, Lederle Laboratories, American Cyanamid Company, Pearl River, New York 10965.
Mendola
C E
CE
Kovesdi
I
I
Fairhurst
J L
JL
O'Hara
B
B
Eddy
R L
RL
Shows
T B
TB
Mathew
S
S
Murty
V V
VV
Chaganti
R S
RS
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D016147:Genes, Tumor Suppressor; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis
D011994:Recombinant Proteins; D009701:Nucleoside-Diphosphate Kinase