Activation of phosphatidylinositol-3 kinase by ligation of the interleukin-7 receptor on human thymocytes.
Interleukin-7 (IL-7) is a glycoprotein that regulates lymphocyte precursor growth and differentiation. However, the exact mechanism whereby the IL-7 receptor (IL-7R) mediates these cell growth signals remains unknown. One of the earliest metabolic events linked to mitogenic responses in other growth factor receptor systems is the activation of phosphatidylinositol-3 kinase (PI-3 kinase). We demonstrate here that ligation of the IL-7R results in dose- and time-dependent increases in PI-3 kinase activity. These results suggest that PI-3 kinase is involved in signal transduction via the IL-7R in human thymocytes.
Dadi
H K
HK
Division of Immunology/Allergy, Hospital for Sick Children, Toronto, Ontario, Canada.
Roifman
C M
CM
D002478:Cells, Cultured; D002648:Child; D004789:Enzyme Activation; D006801:Humans; D066298:In Vitro Techniques; D015851:Interleukin-7 / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases; D010716:Phosphatidylinositols / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000502:physiology*; D020395:Receptors, Interleukin-7; D015398:Signal Transduction; D013950:Thymus Gland / Q000201:enzymology*
D015851:Interleukin-7; D010716:Phosphatidylinositols; D011971:Receptors, Immunologic; D020395:Receptors, Interleukin-7; D010770:Phosphotransferases; D019869:Phosphatidylinositol 3-Kinases