Biodistribution of micelle-forming polymer-drug conjugates.
Polymeric micelles have potential utility as drug carriers. To this end, polymeric micelles based on AB block copolymers of polyethylene oxide (PEO) and poly(aspartic acid) [p(Asp)] with covalently bound Adriamycin (ADR) were prepared. The micelle forming polymer-drug conjugates [PEO-p(Asp(ADR)] were radiolabeled and their biodistribution was investigated after intravenous injection in mice. Long circulation times in blood for some compositions of PEO-p[Asp(ADR)] conjugates were evident, which are usually atypical of colloidal drug carriers. This was attributed to the low interaction of the PEO corona region of the micelles with biocomponents (e.g., proteins, cells). Low uptake of the PEO-p(Asp(ADR)] conjugates in the liver and spleen was determined. The biodistribution of the PEO-p[Asp(ADR)] conjugates was apparently dependent on micelle stability; stable micelles could maintain circulation in blood, while unstable micelles readily formed free polymer chains which rapidly underwent renal excretion. Long circulation times in blood of PEO-p(Asp(ADR)] conjugates are thought to be prerequisite for enhanced uptake at target sites (e.g., tumors).
International Center for Biomaterials Science, Science University of Tokyo, Chiba, Japan.
G S
GS
Kwon
M
M
Yokoyama
T
T
Okano
Y
Y
Sakurai
K
K
Kataoka
D000818:Animals; D004317:Doxorubicin / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D004337:Drug Carriers; D005260:Female; D007275:Injections, Intravenous; D051379:Mice; D008815:Mice, Inbred Strains; D008823:Micelles; D008970:Molecular Weight; D010455:Peptides; D011092:Polyethylene Glycols; D011108:Polymers; D014018:Tissue Distribution
D004337:Drug Carriers; D008823:Micelles; D010455:Peptides; D011108:Polymers; C017645:polyaspartate; D011092:Polyethylene Glycols; D004317:Doxorubicin