Comparison between the properties of 3-nitrosalicyl-N-alkylamide and antimycin A acting on QH2:cytochrome c reductase.
3-Nitrosalicyl N-alkylamide was found to be an inhibitor different from antimycin A not only in its inhibitory nature but also in many other aspects. This difference indicated that the 11 kDa component, which was identified as the antimycin A (AA) binding factor in the QH2: cytochrome c reductase of Rhodopseudomonas sphaeroides by Wilson et al. ((1985) J. Biol. Chem. 260, 10288-10292) using the radioactive photoaffinity analogue 3-azidosalicyl N-octadecylamide, was not the genuine binding site of AA. Based on the observations that the 3-azidosalicyl N-alkylamide specifically inhibits the reactions of ubiquinone catalyzed by Q-related enzymes of the respiratory chain, the labeled 11 kDa factor might be one of the ubiquinone binding proteins in QH2:cytochrome c reductase.
Institute of Biophysics, Academia Sinica, Beijing, China.
J X
JX
Xu
Y
Y
Xiao
Y H
YH
Wang
X
X
Li
L Q
LQ
Gu
D000968:Antimycin A / Q000494:pharmacology*; D001386:Azides / Q000494:pharmacology; D001665:Binding Sites; D002352:Carrier Proteins / Q000032:analysis; D003573:Cytochrome b Group / Q000037:antagonists & inhibitors; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors* / Q000737:chemistry; D010084:Oxidation-Reduction; D012242:Rhodobacter sphaeroides / Q000201:enzymology; D012457:Salicylamides / Q000494:pharmacology*; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology
D001386:Azides; D002352:Carrier Proteins; D003573:Cytochrome b Group; D012457:Salicylamides; D013386:Succinates; C027208:ubiquinone-binding proteins; D014451:Ubiquinone; D000968:Antimycin A; C046280:3-azidosalicyl-N-(n-octadecyl)amide; D019802:Succinic Acid; D014450:Electron Transport Complex III; C003741:ubiquinol