[Evaluation of the biocompatibility of implant materials with human bone marrow cell cultures].
A cell culture biocompatibility test for implant materials is described. For the first time, the human bone marrow cells that actually come into direct contact with the implanted biomaterial can be employed. The usefulness of this method is shown for materials which have long been used in the clinical situation (hydroxyapatite, commercially pure titanium and ultra-high molecular weight polyethylene). After two weeks in culture, light- and fluorescence-microscopic and SEM studies revealed significant differences in cell growth. Maximum compatibility was shown by the hydroxyapatite specimen. In the case of commercially pure titanium we found somewhat decreased biocompatibility. Polyethylene was associated with distinct signs of cytotoxicity. These results are compatible with those of animal experiments and clinical experience. Our system would thus appear to be a useful screening test for some implantation materials, and may help reduce the need for animal tests.
Orthopädische Klinik, Philipps-Universität Marburg.
A
A
Wilke
G
G
Schröder
J
J
Orth
P
P
Griss
R P
RP
Franke
D001672:Biocompatible Materials; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000633:toxicity; D017886:Durapatite; D005109:Extracellular Matrix / Q000187:drug effects; D006801:Humans; D006882:Hydroxyapatites / Q000633:toxicity; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011095:Polyethylenes / Q000633:toxicity; D014025:Titanium / Q000633:toxicity
D001672:Biocompatible Materials; D006882:Hydroxyapatites; D011095:Polyethylenes; D017886:Durapatite; D014025:Titanium