Axotomy increases CNTF receptor mRNA in rat spinal cord.
In order to study the role of ciliary neurotrophic factor (CNTF) and its receptor (CNTF-R) in the response of spinal cord neurons to axotomy, we measured the levels of CNTF mRNA in nerve and CNTF-R mRNA in spinal cord following transection of sciatic nerve, using reverse transcriptase PCR. We found CNTF mRNA levels in the nerve fell and that CNTF-R mRNA levels in spinal cord increased at both 1 and 7 days following transection.
Department of Neurology, University of Michigan, Ann Arbor 48105.
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D000818:Animals; D001369:Axons / Q000502:physiology*; D001483:Base Sequence; D020934:Ciliary Neurotrophic Factor; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D020801:Receptor, Ciliary Neurotrophic Factor; D011956:Receptors, Cell Surface / Q000235:genetics*; D013116:Spinal Cord / Q000378:metabolism*
D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D011956:Receptors, Cell Surface