The products of the E5, E6, or E7 open reading frames of RhPV 1 can individually transform NIH 3T3 cells or in cotransfections with activated ras can transform primary rodent epithelial cells.
Rhesus papillomavirus (RhPV) type 1 was recently shown to cooperate with the activated ras oncogene to transform primary rodent epithelial cells at a level comparable to HPV 16. In similar cotransfection studies, subgenomic portions of RhPV 1 driven by either their natural or a strong heterologous promoter were used in primary baby rat kidney cells to demonstrate that transforming properties of RhPV 1 could be localized individually to the E5, E6, and E7 open reading frames. Fully transformed cells were observed when either E5 or E7 were downstream of a strong heterologous promoter. Similarly, either E6 or E6 and E7 downstream of the native promoter fully transformed these cells as determined by immortalization, anchorage independent growth and tumorigenicity studies.
Ostrow
R S
RS
Institute of Human Genetics, University of Minnesota Medical School, Minneapolis 55455.
Liu
Z
Z
Schneider
J F
JF
McGlennen
R C
RC
Forslund
K
K
Faras
A J
AJ
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014162:Transfection
D009841:Oligonucleotides; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger