Sequence variation in the noncoding region of human papillomavirus type 16 detected by single-strand conformation polymorphism analysis.
Human papillomavirus (HPV) type 16 variants were found by single-strand conformation polymorphism (SSCP) analysis of the noncoding region of the viral genome. Two sets of primers were used to analyze a 1018 bp region spanning nucleotides 7109-222. Twelve SSCP patterns were demonstrated among HPV 16 DNAs purified from 48 anal specimens from 24 homosexual men. Seven patterns were detected among 10 HPV 16 isolates from cervical carcinomas. In two pairs of sex partners, identical variants were recognized in each partner of the pair. Infection with two variants of HPV 16 from 2 specimens was observed in 1 of 21 subjects for whom there were multiple samples over time. DNA sequence analysis of 7 isolates confirmed that the SSCP technique showed polymorphisms only in fragments that had base substitutions and that all of these base substitutions resulted in detectable shifts in fragment mobility.
Xi
L F
LF
Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle WA 98109.
Demers
W
W
Kiviat
N B
NB
Kuypers
J
J
Beckmann
A M
AM
Galloway
D A
DA
D001003:Anal Canal / Q000382:microbiology; D001483:Base Sequence; D002277:Carcinoma / Q000382:microbiology; D016358:Contact Tracing; D004277:DNA, Single-Stranded / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014412:Tumor Virus Infections / Q000235:genetics / Q000635:transmission; D002583:Uterine Cervical Neoplasms / Q000382:microbiology
D004277:DNA, Single-Stranded