New calibration method for gas chromatographic assay of carbon monoxide in blood.
The calibration method described is based on the in situ formation of carbon monoxide in a closed system by the reaction of hot concentrated sulfuric acid with formic acid. Carbon monoxide is released from hemoglobin by treatment with 85% phosphoric acid, diluted 1:2 (v/v). Carbon monoxide is analyzed by means of headspace gas chromatography on a Porapak Q 80-100 column, following catalytic reduction to methane, using a flame-ionization detector. The method was validated by comparing the results obtained for blood samples from patients suffering from carbon monoxide poisoning and previously analyzed by means of spectrophotometry.
Cardeal
Z L
ZL
Laboratoire de Chimie Analytique, Faculté des Sciences Pharmaceutiques, Université de Paris XI, Chatenay Malabry, France.
Pradeau
D
D
Hamon
M
M
Abdoulaye
I
I
Pailler
F M
FM
Lejeune
B
B
D002138:Calibration; D002248:Carbon Monoxide / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans
D002248:Carbon Monoxide