Bone-derived growth factor release from poly(alpha-hydroxy acid) implants in vitro.
Matrix proteins were extracted from bovine cortical bone and polymer implant discs (13 mm x 2 mm composed of 50:50 poly DL-lactide-co-glycolide; mol. wt. approximately 9000) prepared by compression moulding granules with lyophilized bone matrix extracts (BMX) 10.1 (w/w). BMX-containing polymers were cultured for 5 wk in either serum-free Dulbecco's modification of Eagle's medium (DMEM) or phosphate buffer, and growth factor activity released into the media assayed by its ability to stimulate the proliferation of murine fibroblast BALB/c/3T3 cells. Approximately 60-75% of the biological activity was released during the first week of culture; however, less than half of the growth factor units originally incorporated into the implants retained biological activity. Scanning electron microscopy revealed the development of significant internal porosity by week 2; the size of the channels, pores and surface openings suggested they were of the right order for bone ingrowth. These preliminary findings suggest that poly(alpha-hydroxy acid) polymers containing bone-derived growth factors could have potential for stimulating osseous regeneration in vivo.
Meikle
M C
MC
Department of Orthodontics, University of London, UK.
Mak
W Y
WY
Papaioannou
S
S
Davies
E H
EH
Mordan
N
N
Reynolds
J J
JJ
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000378:metabolism*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D005240:Feasibility Studies; D005347:Fibroblasts; D006133:Growth Substances / Q000378:metabolism*; D007773:Lactates; D019344:Lactic Acid; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011091:Polyesters; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D019736:Prostheses and Implants
D001672:Biocompatible Materials; D006133:Growth Substances; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)