Protection of ROC-1 hybrid glial cells by polyethylene glycol following ATP depletion.
Oligodendroglia-glioma hybrid cells (ROC-1) subjected to inhibition of glycolytic and oxidative ATP synthesis undergo a sequence of changes, including ATP depletion, parallel processes of cell swelling and blebbing, and finally plasma membrane disruption and cell death. The morphological and biochemical changes that follow ATP depletion were studied in the presence and absence of polyethylene glycol (M(r) 8,000), a nonpermeant oncotic agent. Polyethylene glycol prevented cell swelling and membrane blebbing. It significantly delayed, but did not prevent, the release of lactate dehydrogenase into the medium; it did not affect the fall in [ATP]. These results suggest that osmotic cell swelling may be a contributing factor in the loss of cell viability when ROC-1 cells are depleted of ATP.
Department of Medical Biochemistry, Ohio State University, Columbus 43210.
M S
MS
Jurkowitz-Alexander
R A
RA
Altschuld
S E
SE
Haun
R E
RE
Stephens
L A
LA
Horrocks
D000255:Adenosine Triphosphate / Q000172:deficiency*; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004734:Energy Metabolism; D005910:Glioma / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism*
D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase