A double-cos-site vector containing a multiple cloning site flanked by T7 and T3 RNA polymerase promoters.
A double-cos-site cosmid vector, c2XMCS, contains a multiple cloning site (MCS) with fifteen restriction sites flanked by phage T7 and T3 RNA polymerase promoters. The two cos sites in the cosmid vector enable the construction of cosmid libraries from unfractionated partial digests of genomic DNA. This simple construct combines the efficiency of a double-cos-site cosmid with the versatility provided by the MCS from pBluescript.
United States Department of Agriculture, Avian Disease and Oncology Laboratory, East Lansing, MI 48823.
J D
JD
Reilly
R F
RF
Silva
D000818:Animals; D017123:Bacteriophage T7 / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015723:Gene Library; D005822:Genetic Vectors; D008381:Herpesvirus 2, Gallid / Q000235:genetics; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014422:Turkeys
D012321:DNA-Directed RNA Polymerases