Rapid and convenient purification of tonin from rat submandibular gland. Comparison of tonin and tissue kallikrein in their interactions with inhibitors.
Tonin was isolated from rat submandibular glands by a very convenient procedure consisting of sequential anion-exchange, hydrophobic interaction and gel filtration chromatographies. The method is superior to earlier purifications as it consists of fewer stages, resulting in a much higher recovery (41%) of tonin. The final preparation was seen to be pure on SDS gel electrophoresis (M(r) 32,800) and on gel isoelectric focusing (pI 6.15). The stability of tonin and its interaction with various inhibitors were investigated, and compared with the corresponding behavior of rat tissue kallikrein.
Department of Chemistry and Biological Chemistry, University of Essex, Colchester, UK.
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D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D002845:Chromatography / Q000379:methods; D004795:Enzyme Stability; D005069:Evaluation Studies as Topic; D066298:In Vitro Techniques; D007610:Kallikreins / Q000037:antagonists & inhibitors; D007700:Kinetics; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013363:Submandibular Gland / Q000201:enzymology*
D000806:Angiotensin-Converting Enzyme Inhibitors; D007703:Peptidyl-Dipeptidase A; D007610:Kallikreins