A gene encoding the 16-kDa proteolipid subunit of Enterococcus hirae Na(+)-ATPase complex.
By further sequencing the cloned genome DNA of Enterococcus hirae that retains the genes encoding two major subunits of Na(+)-transport ATPase (Takase, K., Yamato, I., and Kakinuma, Y. (1993) J. Biol. Chem. 268, 11610-11616), we found a gene, ntpK, encoding a very hydrophobic protein of 156 amino acids (Mr = 16,036). The amino acid sequence deduced from the ntpK gene matched with the partial sequence of a 16-kDa protein purified as the proteolipid component of Na(+)-ATPase. The amino acid sequence and the hydropathy profile of the NtpK product were very similar with those of the 16-kDa proteolipids of vacuolar (V-) H(+)-ATPases in eukaryotes. The amino-terminal half of this sequence was highly homologous to the carboxyl-terminal half, suggesting that it was evolved from a common ancestral gene through duplication. The proteolipid of E. hirae Na(+)-ATPase belongs to that of the eukaryotic V-ATPase.
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Y
Y
Kakinuma
S
S
Kakinuma
K
K
Takase
K
K
Konishi
K
K
Igarashi
I
I
Yamato
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D027682:Cation Transport Proteins; D002417:Cattle; D002837:Chromaffin Granules / Q000201:enzymology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D016983:Enterococcus / Q000201:enzymology* / Q000235:genetics*; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009876:Operon; D011487:Protein Conformation; D011510:Proteolipids / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D016945:Sulfolobus acidocaldarius / Q000201:enzymology / Q000235:genetics
D027682:Cation Transport Proteins; D004269:DNA, Bacterial; D046911:Macromolecular Substances; D011510:Proteolipids; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase