Unfolding of nucleosome cores dramatically changes the distribution of ultraviolet photoproducts in DNA.
Nucleosome core particles undergo a conformational change at ionic strengths below 0.2 mM; the fluorescence anisotropy decay of bound ethidium indicates that under these conditions the particle adopts a highly extended structure. We have measured the distribution of UV-induced DNA damage (primarily cyclobutane-pyrimidine dimers) through a process termed photofootprinting. As the core particle is exposed to ionic strengths below 0.2 mM, the photofootprint pattern changes from that observed for native cores, with a characteristic 10.3 base repeat pattern presumably derived largely from the bending of DNA around the histone octamer, to a more evenly distributed pattern resembling that of free DNA. These results provide clear evidence that the DNA in the core particle at these very low ionic strengths, although still tightly bound to histones, is no longer bent to a significant degree.
Brown
D W
DW
Washington State University, Pullman, Washington 99164-4660.
Libertini
L J
LJ
Suquet
C
C
Small
E W
EW
Smerdon
M J
MJ
D000818:Animals; D002645:Chickens; D004247:DNA / Q000528:radiation effects* / Q000648:ultrastructure; D004249:DNA Damage; D004912:Erythrocytes / Q000648:ultrastructure; D008961:Models, Structural; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D009707:Nucleosomes / Q000528:radiation effects / Q000648:ultrastructure*; D009994:Osmolar Concentration; D014466:Ultraviolet Rays
D009707:Nucleosomes; D004247:DNA