Conventional and enantioselective determination of a new blood glucose-lowering agent in biological fluids using liquid-liquid extraction and high-performance liquid chromatography.
Two analytical methods are described for the determination of 2-(4-tert.-butylphenoxy)-7-(4-chlorophenyl)heptanoic acid sodium salt (I) in animal models (beagle dog and rat). Method 1 is conventional reversed-phase high-performance liquid chromatography on an octadecylsilane column with an eluent of acetonitrile-0.02 M potassium buffer (pH 3) (65:35, v/v). Method 2 is used for the enantioselective determination of I. This method uses a chiral column (Chiralcel OJ) with an eluent of n-hexane-2-propanol (95:5, v/v) containing 3 ml/l trifluoracetic acid. The analytical procedure has a recovery of more than 90%; within-run precision of less than 5.1%, and between-run precision of less than 4.3%.
Neidlein
R
R
Pharmaceutical-Chemical Institute, University of Heidelberg, Germany.
Heitzmann
T
T
Strein
K
K
Volland
C
C
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D006538:Heptanoic Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D007004:Hypoglycemic Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D006538:Heptanoic Acids; D007004:Hypoglycemic Agents; D007202:Indicators and Reagents; C083311:2-(4-tert-butylphenoxy)-7-(4-chlorophenyl)heptanoic acid