Beta-adrenergic receptors in human anterior optic nerve: an autoradiographic study.
Sections of human anterior optic nerve and nerve head were incubated in a physiological solution containing a radiolabelled beta blocker at a low concentration. The beta blocker used was (-)-(125iodo)-cyanopindolol, which has a high affinity and specificity for beta-adrenergic receptors. Concurrent incubations were performed with a great excess of unlabelled beta blocker added to demonstrate non-specific binding. Following incubation the sections were washed and dried. They were then apposed to photographic film for 5 days and developed. Incubations were performed with the stereoisomers of propranolol and an alpha blocker as well as specific beta-one and beta-two blockers. Beta-adrenergic receptors were demonstrated in anterior optic nerve and optic nerve head. The majority were of the beta-two subtype.
Department of Ophthalmology, University Hospital of Wales, Cardiff.
G M
GM
Dawidek
M I
MI
Robinson
D001345:Autoradiography; D006801:Humans; D020120:Iodocyanopindolol; D009898:Optic Disk / Q000737:chemistry; D009900:Optic Nerve / Q000737:chemistry*; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011433:Propranolol / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000032:analysis* / Q000378:metabolism
D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D011433:Propranolol; D010869:Pindolol