Cytomegalovirus infection of the BS-1 human stroma cell line: effect on murine hemopoiesis.
BS-1, a stromal cell line derived from human bone marrow, can support the growth of murine erythroid (BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-M) progenitor cells in a short term in vitro coculture system. Exposure of BS-1 cells to cytomegalovirus (CMV) for 3 hr prior to coculture results in a marked reduction in the stroma cell's ability to support murine hemopoiesis. CMV's effect on the BS-1 cell's hematopoietic support function is dependent on the multiplicity of infection with total suppression of BFU-E observed at a 1:1 ratio of virus to bone marrow cells. A 50% loss in the ability of BS-1 cells to support BFU-E is observed at a 0.1:1 ratio. No effect of CMV is observed with further log dilutions of virus. CMV infection of BS-1 cells affects its support of erythroid progenitor cell growth to a greater extent than its influence on the development of granulocyte-macrophage colonies. Antibody to CMV or heat inactivation of the virus reverses the inhibitory affect on BS-1 cells. The results suggest that CMV can infect a cell that constitutes one of the cellular elements of the normal bone marrow microenvironment causing a decrease in the stroma's ability to support the growth and development of normal progenitor cells.
Harvard Thorndike Laboratory, Charles A. Dana Research Institute, Beth Israel Hospital, Boston, Massachusetts 02215.
H N
HN
Steinberg
J
J
Anderson
B
B
Lim
P A
PA
Chatis
D000818:Animals; D001853:Bone Marrow / Q000382:microbiology*; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D003587:Cytomegalovirus / Q000254:growth & development*; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000382:microbiology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D017154:Stromal Cells / Q000382:microbiology*