Impairment of anti-platelet-aggregating activity of endothelial cells after experimental subarachnoid hemorrhage.
OBJECTIVE
Serial changes of anti-platelet-aggregating activity in the endothelial cells after experimental subarachnoid hemorrhage were studied in 30 feline two-hemorrhage models.


METHODS
One hour or 2, 4, 7, or 14 days after mimic subarachnoid hemorrhage, ADP (40 mg/kg) was infused into the basilar artery via the right vertebral artery to activate circulating platelets. Immediately after ADP infusion, the basilar artery was fixed by intra-arterial perfusion with 1.5% glutaraldehyde in 0.1 mol/L phosphate buffer and was removed. The luminal surface was examined under a scanning electron microscope.


RESULTS
One hour after subarachnoid hemorrhage, no platelets adhered or aggregated on the luminal surface. However, 4 to 7 days after subarachnoid hemorrhage, many platelets were observed adhering or aggregating on the luminal surface.


CONCLUSIONS
These findings suggest the impairment of anti-platelet-aggregating activity of endothelial cells after subarachnoid hemorrhage. This impairment may be involved in inducing cerebral ischemia during cerebral vasospasm by causing platelet adhesion and aggregation.
Department of Neurosurgery, Hirosaki University School of Medicine, Japan.
H
H
Ohkuma
K
K
Ogane
S
S
Fujita
H
H
Manabe
S
S
Suzuki
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D002404:Catheterization; D002415:Cats; D004730:Endothelium, Vascular / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D013345:Subarachnoid Hemorrhage / Q000097:blood* / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors
D000244:Adenosine Diphosphate