Convenient syntheses of oligonucleotides linked to 5-deazaflavin coenzyme models at 3'-end. Incorporation of 5-deazaflavin to controlled pore glass (CPG) support.
Using CPG support linked with 5-deazaflavin, the 5-deazaflavin modified oligodeoxynucleotides at 3'-end(ODN-dF1) were synthesized. The thermal stability of the duplex of ODN-dF1 with its complement was higher than that of oligonucleotide linked to 5-deazaflavin at 5'-end internucleotide linkage.
Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Y
Y
Nakamura
T
T
Akiyama
K
K
Bessho
F
F
Yoneda
D001483:Base Sequence; D003067:Coenzymes / Q000737:chemistry*; D015342:DNA Probes / Q000138:chemical synthesis; D005415:Flavins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*
D003067:Coenzymes; D015342:DNA Probes; D005415:Flavins; D009841:Oligonucleotides; C019319:5-deazaflavin