Immunological detection of F1-ATPase gamma-subunit in rat liver peroxisomes.
The gamma-subunit of F1-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F1-ATPase. Other subunits of F1-ATPase such as alpha, beta and delta were not detected in the peroxisomes. The polypeptide corresponding to the gamma-subunit in peroxisomes was also recognized by affinity purified antibody against F1-ATPase gamma-subunit. The amount of gamma-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of alpha, beta and delta-subunits were not increased. These results suggest that the gamma-subunit of F1-ATPase is a constituent of peroxisomes in rat liver.
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
T
T
Imanaka
Y
Y
Shiina
Y
Y
Moriyama
S
S
Ohkuma
T
T
Takano
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002458:Cell Fractionation / Q000379:methods; D002846:Chromatography, Affinity; D002994:Clofibrate / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D046911:Macromolecular Substances; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D006180:Proton-Translocating ATPases / Q000032:analysis* / Q000378:metabolism; D051381:Rats
D000906:Antibodies; D046911:Macromolecular Substances; D006180:Proton-Translocating ATPases; D002994:Clofibrate