Protective immune response of Isospora felis-infected mice against Babesia microti infection.
Protective response against Babesia microti was studied in Isospora felis-infected mice. Isospora felis-infected mice which were exposed to B. microti on the 28th day post-infection showed absolute resistance against Babesia microti. Interestingly, these mice showed no anti-B. microti antibodies. Mice that received spleen cells from I. felis-infected donors that were subsequently exposed to B. microti showed lower peak parasitemia (10.3% +/- 2.6) compared to those mice that received normal spleen cells (60.9% +/- 15.0), and no spleen cells at all (47.3% +/- 8.5). Treatment of I. felis-infected mice with monoclonal antibodies against L3T4+ cells resulted to a depression of their resistance to B. microti, as clearly manifested by high levels of parasitemia. Findings of the present study demonstrate the role of cell mediated immunity, specifically by L3T4+ T-cells induced by I. felis infection, in providing mice protection against B. microti.
Takahashi
M
M
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Omata
Y
Y
Oikawa
H
H
Claveria
F
F
Igarashi
I
I
Saito
A
A
Suzuki
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000913:Antibodies, Protozoan / Q000097:blood; D001404:Babesiosis / Q000276:immunology*; D002415:Cats; D003048:Coccidiosis / Q000276:immunology*; D004912:Erythrocytes / Q000469:parasitology; D005260:Female; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000097:blood; D016219:Immunotherapy, Adoptive; D007549:Isospora / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D007074:Immunoglobulin G