Properties of ADP-degrading activity of human placental syncytiotrophoblast brush border membrane vesicles.
The enzymatic properties of ADP (adenosine diphosphate) degradation in human placental syncytiotrophoblast brush border membrane vesicles (BBMV) were explored and the following results were obtained. BBMV had high ADP degrading activity compared to homogenate of placental villi. ADP degrading activity of BBMV; 1.05 +/- 0.05 mumol/mg protein/min placental villi was 21 times higher than that of homogenate of placental villi. Hydrolysis of ADP by BBMV follows Michaelis-Menten saturation kinetics with an apparent Km of 10.9 +/- 0.8 microM and Vmax of 2.10 +/- 0.17 mumol/mg protein/min. The enzyme has a divalent cation requirement. EDTA (2 mM) was found to abolish ADP degrading activity but this could be restored by the addition of either magnesium or calcium ions. Maximum enzyme activity of ADP degradation in BBMV was observed at a pH close to 8.0. The enzyme was insensitive to vanadate, levamisole, oligomycin, ouabain and N-ethylmaleimide (NEM), omeprazole and adenosine (5') pentaphospho (5') adenosine.
Department of Obstetrics and Gynecology Nara Medical University, Japan.
H
H
Iioka
S
S
Akada
Y
Y
Sakamoto
T
T
Shimamoto
Y
Y
Yamada
I S
IS
Moriyama
M
M
Ichijo
D000244:Adenosine Diphosphate / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002824:Chorionic Villi / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D000244:Adenosine Diphosphate; D004492:Edetic Acid; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium; D002118:Calcium