c-myc gene activation as a permanent trait of RSV-infected quail cells.
A high level of c-myc gene expression was found to be a constant feature of v-src transformation. The c-myc gene product was analyzed in quail embryo cells transformed by different mutants of Rous sarcoma virus (RSV) that were temperature-sensitive with respect to various parameters of v-src function. The high-level expression of c-myc proved not to be temperature-sensitive: at both permissive (35 degrees C) and non-permissive (41 degrees C) temperatures, the same high levels of c-myc gene product were found for all RSV mutants tested. This result, in agreement with earlier evidence for a v-src-induced proliferative stimulus which was unaffected by ts mutants at the non-permissive temperature, shows that certain v-src functions have not yet been fully characterized.
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
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Sovová
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Friis
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Fidlerová
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Hlozánek
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000235:genetics; D003370:Coturnix / Q000196:embryology* / Q000382:microbiology*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D010641:Phenotype; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D001357:Sarcoma, Avian / Q000235:genetics* / Q000382:microbiology; D012680:Sensitivity and Specificity; D013696:Temperature; D015533:Transcriptional Activation
D016271:Proto-Oncogene Proteins c-myc