Selective antibodies to propranolol enantiomers produced from a new conjugate.
A selective antibody to (S)-propranolol enantiomer was produced in rabbits by immunization with a new conjugate of N-aminopropylpropranolol-albumin. A hapten was first prepared by condensing (S)-propranolol or the racemate with 3-bromopropylphthalimide followed by hydrazinolysis, and the resulting compound conjugated to serum albumin by means of a glutaraldehyde- or carbodiimide-mediated reaction. Rabbits were immunized, and titres and specificity of antibodies were determined by ELISA. The antibodies obtained were tested with (S)-, (R)-, (R,S)-propranolol, and other structural analogs. Selective (S)-antibodies recognized (S)-propranolol 20 times more avidly than (R)-isomer while an antiserum against (R,S)-propranolol recognized both (S)- and (R)-isomers to about the same degree.
Laboratoire de Toxicologie, Faculté de Pharmacie, Paris, France.
A
A
Sahui-Gnassi
C
C
Pham-Huy
H
H
Galons
J M
JM
Warnet
J R
JR
Claude
H T
HT
Duc
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D006241:Haptens; D007114:Immunization; D011433:Propranolol / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology; D013237:Stereoisomerism
D000906:Antibodies; D006241:Haptens; D012709:Serum Albumin; D011433:Propranolol