Bicarbonate in the treatment of metabolic acidosis: effects on hepatic intracellular pH, gluconeogenesis, and lactate disposal in rats.
The effects of agents used in the treatment of metabolic acidosis could depend on the induced changes in intracellular pH (pHi). To determine the effect of sodium bicarbonate on hepatic pHi and function, this agent was infused into anesthetized rats with acute metabolic acidosis due to either diabetic ketoacidosis (DKA) or HCl infusion. Hepatic pHi was measured by 31P-magnetic resonance spectroscopy (MRS). A substantial increase in pHi occurred (from 7.13 +/- 0.08 to 7.32 +/- 0.08, P < .05) despite an increase in mixed venous PCO2. Isolated livers from normal rats or those with DKA were perfused at pH 6.8 and normal PCO2. With infusion of sodium bicarbonate, there was again an increase in pHi (delta pHi, + 0.27 +/- 0.06, P < .02) despite increases in both portal and hepatic venous PCO2. Lactate uptake was increased twofold to threefold (P < .001) by bicarbonate infusion in perfusions from both types of animals. Glucose output was increased twofold (P < .001) only in livers from normal animals.
Cellular Mechanisms Research Group, London Hospital Medical College, UK.
J S
JS
Beech
R A
RA
Iles
R D
RD
Cohen
D000138:Acidosis / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D001639:Bicarbonates / Q000627:therapeutic use*; D016883:Diabetic Ketoacidosis / Q000188:drug therapy* / Q000378:metabolism*; D005943:Gluconeogenesis / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism*; D008099:Liver / Q000737:chemistry* / Q000473:pathology / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000627:therapeutic use*; D017693:Sodium Bicarbonate
D001639:Bicarbonates; D007773:Lactates; D017693:Sodium Bicarbonate; D012964:Sodium; D005947:Glucose; D006851:Hydrochloric Acid