Structure of the promoter region of the gene encoding cytochrome c oxidase subunit V in Dictyostelium.
A cDNA for the nuclear-encoded subunit V of Dictyostelium discoideum cytochrome-c oxidase was used as a probe to screen a genomic library and isolate the complete gene. Primer-extension analysis revealed two transcription start sites located 32 and 39 nucleotides upstream of the translation initiation codon. The chloramphenicol acetyltransferase assay in transient and stable Dictyostelium transformants indicated that the 400-bp dT-rich segment 5' to the transcription start sites retained promoter activity. This region contains an octanucleotide sequence similar to the yeast HAP2/3/4 responsive element.
CNR Centro Studi per la Fisiologia dei Mitocondri, Università di Padova, Italy.
R
R
Rizzuto
D
D
Sandonà
M
M
Brini
R
R
Marschalek
T
T
Dingermann
R
R
Bisson
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004271:DNA, Fungal / Q000737:chemistry; D004023:Dictyostelium / Q000201:enzymology / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D004271:DNA, Fungal; D003576:Electron Transport Complex IV