[Signal transduction of insulin-like growth factor I in a highly metastatic tumor cell line].
Insulin-like growth factor I (IGF-I) exists in the blood at a high concentration and in various tissues. IGF-I may positively participate in the process of tumor metastasis, since it has various biological activities in addition to growth promoting activity for many cell types including malignant cells. We found a highly metastatic cell line (colon 26 NL-17) the growth of which IGF-I strongly promoted. Investigation of the mechanism of signal transduction of IGF-I in the NL-17 cells revealed two cytoplasmic proteins (pp150 and pp160) which were immediately tyrosine-phosphorylated upon IGF-I stimulation and enabled to bind to phosphatidyl inositol 3-kinase. The pp150 and pp160 may be important mediators of IGF-I signal.
Cancer Chemotherapy Center, Japanese Foundation for Cancer Research.
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Yamori
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Tsuruo
D000818:Animals; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000502:physiology*; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000502:physiology; D019869:Phosphatidylinositol 3-Kinases; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism
D009363:Neoplasm Proteins; D014443:Tyrosine; D007334:Insulin-Like Growth Factor I; D010770:Phosphotransferases; D019869:Phosphatidylinositol 3-Kinases