Relative roles of testosterone and the germ cell complement in determining stage-dependent changes in protein secretion by isolated rat seminiferous tubules.
This study has compared the effect of withdrawal of testosterone (+/- replacement) with that of selective depletion of pachytene spermatocytes (PS) or round/elongating spermatids (RS), or both PS and RS, on the level of overall protein secretion by seminiferous tubules (ST) isolated at particular stage-groups of the spermatogenic cycle. Testosterone withdrawal was induced by destroying the Leydig cells with a single injection of ethane-dimethane sulphonate (EDS), with or without concomitant replacement of testosterone by injection; ST at stages II-V, VI-VIII or IX-XII were then isolated from control and treated rats at 4 days after treatment. Methoxyacetic acid (MAA) was administered, in either one or two doses, to selectively destroy 80-100% of pachytene and later spermatocytes; ST at stages I-V, VI-VIII or IX-XIV were then isolated at specific times after treatment such that ST were depleted selectively of either PS, RS or PS+RS. Isolated ST (5 cm) were then cultured for 22 h at 34 degrees C with 35S-methionine and its incorporation into secreted proteins then quantified. Based on the incorporation of 35S-methionine, ST at stages VI-VIII showed a significantly higher level of protein secretion than did ST at earlier or later stages. This difference was abolished following testosterone withdrawal but was maintained by testosterone replacement. The normal increase in protein secretion by ST at stages VI-VIII was also prevented if either PS or RS were depleted, whereas depletion of either PS or RS alone had no significant effect on protein secretion by ST at stages I-V, and only the depletion of RS significantly reduced protein secretion by ST at stages IX-XIV. Depletion of both PS+RS reduced protein secretion significantly by ST at all stages. In contrast to the data for total protein secretion, the levels of sulphated glycoprotein (SGP)-1 and SGP-2 secreted by ST at stages VI-VIII showed that these two Sertoli cell proteins were unaffected by germ cell depletion except after co-depletion of PS+RS when secretion of SGP-1 was halved.(ABSTRACT TRUNCATED AT 400 WORDS)
Sharpe
R M
RM
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK.
Millar
M
M
McKinnell
C
C
D000085:Acetates; D000818:Animals; D051152:Clusterin; D046508:Culture Techniques; D006023:Glycoproteins / Q000378:metabolism*; D008297:Male; D008698:Mesylates; D018832:Molecular Chaperones; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000033:anatomy & histology / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology*; D013739:Testosterone / Q000502:physiology*
D000085:Acetates; D051152:Clusterin; D006023:Glycoproteins; D008698:Mesylates; D018832:Molecular Chaperones; D013739:Testosterone; C002994:ethylene dimethanesulfonate; C013598:methoxyacetic acid