Feline CD4 molecules expressed on feline non-lymphoid cell lines are not enough for productive infection of highly lymphotropic feline immunodeficiency virus isolates.
To investigate whether the feline CD 4 (fCD4) molecules are involved in infections of highly lymphotropic feline immunodeficiency virus (FIV) isolates, we expressed fCD4 stably on Crandell feline kidney cells and Felis catus whole foetus 4 cells by transfection of a cDNA encoding the fCD4 glycoprotein, and then infected them with TM 1 and TM 2 strains of FIV, which are unable to infect these cells productively. In spite of fCD 4 being expressed on these cells, no virus production was observed. This result indicates that fCD 4 expression alone cannot induce a productive infection of the FIV TM 1 and TM 2 strains.
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
J
J
Norimine
T
T
Miyazawa
Y
Y
Kawaguchi
K
K
Tomonaga
Y S
YS
Shin
T
T
Toyosaki
M
M
Kohmoto
M
M
Niikura
Y
Y
Tohya
T
T
Mikami
D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D002415:Cats / Q000276:immunology*; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D005333:Fetus; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015750:Genes, gag; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D007668:Kidney; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D014162:Transfection
D015704:CD4 Antigens; D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins