Analysis of hapten-carrier protein conjugates by nondenaturing gel electrophoresis.
Hapten-carrier protein conjugates were made using five different small haptens (MW < 1000), two carrier proteins and two methods of conjugation. Nondenaturing agarose gel electrophoresis was used to demonstrate that when as few as two molecules of a small hapten are attached to the carrier, the conjugate band migrates differently from that of the carrier alone or of the coupling reagent-treated carrier. Furthermore, the direction of the change in migration of each conjugate correlates with the change in charge which occurs upon attachment of the hapten to the carrier.
Food Animal Protection Research Laboratory, U.S. Department of Agriculture, College Station, TX 77845.
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Kamps-Holtzapple
R J
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Carlin
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Sheffield
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Kubena
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Stanker
J R
JR
DeLoach
D002934:Cinnamates; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005029:Ethylenediamines / Q000737:chemistry; D037341:Fumonisins; D005665:Furosemide / Q000737:chemistry; D006241:Haptens / Q000032:analysis*; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000737:chemistry; D009183:Mycotoxins / Q000737:chemistry; D010047:Ovalbumin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D015025:Zearalenone / Q000031:analogs & derivatives / Q000737:chemistry
D002934:Cinnamates; D005029:Ethylenediamines; D037341:Fumonisins; D006241:Haptens; D009183:Mycotoxins; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D006921:Hygromycin B; C026273:hygromycin A; C056933:fumonisin B1; D015025:Zearalenone; C031234:ethylenediamine; D005665:Furosemide; D010047:Ovalbumin