Multianalyte assay system developed for drugs of abuse.
A simple, 10-min immunoassay system has been developed that simultaneously screens for five different classes of drugs of abuse in a urine sample. This system tests for amphetamines, cannabinoids, cocaine metabolites, opiates, and phencyclidine, and each assay has a specific preset cutoff concentration. Accuracy is > 99% for reporting positive or negative results for samples with 200% or 50%, respectively, of the cutoff concentrations of the drugs. Tests of a panel of 96 compounds yielded only three cases of nonspecific reactivity (at a drug concentration of 100 mg/L). Another panel of 12 compounds that could normally be found in urine samples was also evaluated and no interferences were observed. Concordance was > 95% between this system and the comparable automated immunoassays for detecting drugs of abuse. Greater than 98% of GC/MS-confirmed positive samples gave positive results with this assay system.
Parsons
R G
RG
Diagnostics Division, Abbott Laboratories, North Chicago, IL 60064.
Kowal
R
R
LeBlond
D
D
Yue
V T
VT
Neargarder
L
L
Bond
L
L
Garcia
D
D
Slater
D
D
Rogers
P
P
D001322:Autoanalysis / Q000295:instrumentation; D006801:Humans; D013287:Illicit Drugs / Q000652:urine*; D007118:Immunoassay / Q000295:instrumentation; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000295:instrumentation*
D013287:Illicit Drugs