Hepatic kinetics and magnetic resonance imaging of gadolinium ethoxybenzyl diethylenetriaminepentacetic acid (Gd-EOB-DTPA) in dogs.
This complex study was designed to measure the transport and excretion characteristics of gadolinium ethoxybenzyl diethylenetriaminepentacetic acid (Gd-EOB-DTPA) in dog's livers following bolus and infusion. Simultaneous T1 magnetic resonance imaging was performed to measure maximum signal enhancement. Anaesthetized dogs had cannulation of the common bile duct and urinary bladder for collections and cannulation of the femoral artery and vein for monitoring, blood sampling and infusion. Gd-EOB-DTPA was administered by bolus (range 12.5-200 mumol/kg) and infusion (range 0.4-6.4 mumol/min per kg). An hepatic transport maximum 0.09-0.15 mumol/min/kg was achieved with a blood concentration of 0.03-0.06 mumol/mL. Marked hepatic affinity for Gd-EOB-DTPA was demonstrated with measurements of liver concentration. Maximum T1 signal enhancement was achieved with blood Gd-EOB-DTPA concentration of 0.02-0.03 mumol/mL and a liver concentration of 1-2 mumol/g. The transport maximum for Gd-EOB-DTPA in the dog was similar to that for ipodate and iodipamide and effective imaging was achieved with sub-maximal doses. The maximum signal enhancement at blood concentrations less than required for maximum transport suggest a wide latitude for effective clinical imaging.
Nuclear Medicine Department, QEII Medical Centre, Nedlands, Western Australia.
G
G
Benness
M
M
Khangure
I
I
Morris
A
A
Warwick
P
P
Burrows
D000818:Animals; D001646:Bile / Q000737:chemistry / Q000378:metabolism; D001769:Blood; D003287:Contrast Media / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D004285:Dogs; D005682:Gadolinium / Q000008:administration & dosage / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D019786:Gadolinium DTPA; D007089:Image Enhancement; D007262:Infusions, Intravenous; D007275:Injections, Intravenous; D008099:Liver / Q000033:anatomy & histology* / Q000737:chemistry / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D010100:Oxygen / Q000097:blood; D004369:Pentetic Acid / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D012636:Secretory Rate; D014556:Urine
D003287:Contrast Media; D009942:Organometallic Compounds; C073590:gadolinium ethoxybenzyl DTPA; D004369:Pentetic Acid; D005682:Gadolinium; D019786:Gadolinium DTPA; D010100:Oxygen