The fission yeast prp4+ gene involved in pre-mRNA splicing codes for a predicted serine/threonine kinase and is essential for growth.
Only four prp (pre-mRNA processing) genes of the fission yeast Schizosaccharomyces pombe have been reported. We exploited yeast genetics and identified and isolated the prp4 gene. Sequence analysis revealed that the splicing factor encoded by this gene contains the signature sequences that define the serine/threonine protein kinase family. This is the first kinase gene identified whose product is involved in pre-mRNA splicing. The prp4 gene contains one intron in the kinase domain. Gene replacement studies provided evidence that this gene is essential for growth and is located on chromosome III.
Alahari
S K
SK
Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104.
Schmidt
H
H
Käufer
N F
NF
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D007438:Introns; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing / Q000235:genetics; D015183:Restriction Mapping; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic; D014158:Transcription, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins; D012322:RNA Precursors; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017346:Protein Serine-Threonine Kinases