Electron transfer from the tetraheme cytochrome to the special pair in isolated reaction centers of Rhodopseudomonas viridis.
Kinetics of electron transfer from the bound tetraheme cytochrome c to the primary donor (P) have been measured in isolated reaction centers of the purple bacterium Rhodopseudomonas viridis by time-resolved flash absorption spectroscopy. The influence of two major parameters has been studied: temperature (7-305 K) and the redox state of the cytochrome. Most experiments were done with one heme (c-559), two hemes (c-559 and c-556), or three hemes (c-559, c-556, and c-552) poised in a reduced state before the flash. Measurements were done at 1283 nm in the absorption band of P+, and in the region of cytochrome alpha-bands. At room temperature, c-559 donates an electron to P+ with a half-time of 115, 190, or 230 ns (with three, two, or one heme reduced, respectively) and is then eventually rereduced by c-556 (t1/2 = 1.7 microseconds) or by c-552 (in less than 40 ns). The kinetics also include a minor microsecond phase of P+ reduction. At decreasing temperatures, the polyphasic character of P+ rereduction is accentuated. Fast phases (115 ns-10 microseconds) are slightly slowed down, following Arrhenius behavior with a weak activation energy (3.6-8.6 kJ.mol-1), until they become temperature-independent. Their extent decreases rather sharply, at temperatures which vary according to the redox poising: 250, 210, or 80 K when one, two, or three hemes are reduced, respectively. In the last case, P+ can still be reduced at low temperature, apparently directly by c-552 (t1/2 = 1.1 ms, nearly temperature-independent).(ABSTRACT TRUNCATED AT 250 WORDS)
CEA/Section de Bioénergétique (CNRS, URA 1290), Gif-sur-Yvette, France.
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Ortega
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Mathis
D003574:Cytochrome c Group / Q000378:metabolism*; D004579:Electron Transport; D007700:Kinetics; D010084:Oxidation-Reduction; D010777:Photochemistry; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000201:enzymology* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D003574:Cytochrome c Group; D045322:Photosynthetic Reaction Center Complex Proteins