Activation of enzymes by calmodulins containing intramolecular cross-links.
We have reacted calmodulins containing cysteines substituted at positions 3 and 146 or 5 and 146 with bismaleimidohexane (BMH) to generate intramolecularly cross-linked proteins termed BMHCM or BMHCM1, respectively. Reactions were also performed with N-ethylmaleimide (NEM) in place of BMH to generate corresponding S-ethylsuccinimidylated proteins termed NEMCM or NEMCM1. The abilities of these proteins to activate plant NAD kinase, erythrocyte Ca(2+)-ATPase and bovine brain calcineurin activities were assessed. The BMH- or NEM-reacted proteins activate calcineurin activity as does control calmodulin. Kact values for Ca(2+)-ATPase activation by BMHCM and BMHCM1 are increased 10-fold relative to the control value, with no corresponding change in Vmax values. Activation of this enzyme by NEMCM or NEMCM1 is not different from the control. In NAD kinase activation experiments BMHCM and BMHCM1 are associated with a 10 to 20-fold increase in Kact values and a 60-75% reduction in Vmax values relative to the control. NEMCM1 is not associated with any apparent changes in NAD kinase activation, however, NEMCM is associated with a 10-fold increase in the Kact value. NEM-reacted calmodulin containing a cysteine only at position 3 is not associated with an increased Kact value, implying that this change is due to interactions between S-(ethylsuccinimido)cysteines at positions 3 and 146. In conclusion, cross-linking and associated distortions in the structure of calmodulin appear to have little or no effect on activation of calcineurin enzyme activity. However, bending in the central helix and/or steric restrictions associated with cross-linking increase significantly the Kact value for Ca(2+)-ATPase and NAD kinase activation, and dramatically reduce maximal activation of NAD kinase activity.
University of Rochester School of Medicine, Department of Physiology, NY 14642.
A
A
Persechini
H W
HW
Jarrett
D
D
Kosk-Kosicka
M H
MH
Krinks
H G
HG
Lee
D001921:Brain / Q000201:enzymology; D019703:Calcineurin; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002147:Calmodulin / Q000031:analogs & derivatives*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D003432:Cross-Linking Reagents; D003545:Cysteine; D004789:Enzyme Activation; D008301:Maleimides / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010944:Plants / Q000201:enzymology
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D003432:Cross-Linking Reagents; D008301:Maleimides; C075594:1,6-bismaleimidohexane; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases; D000252:Calcium-Transporting ATPases; D003545:Cysteine