Autocrine motility factor is a growth factor.
Autocrine motility factor (AMF) is a 55 kDa cytokine which is produced and secreted by cancer cells and which regulates cell motility via binding to its receptor, a 78 kDa cell surface glycoprotein (gp78-AMFR), and activating a pertussis toxin (PT)-sensitive G-protein. AMF purified from HT-1080 human fibrosarcoma cells stimulates the growth and motility of 3T3-A31-fibroblasts at a concentration of 0.1 ng/ml or less. The expression of total as well as cell surface gp78-AMFR is down-regulated in contact-inhibited A31-fibroblasts and AMF stimulates the healing of experimentally wounded, density-arrested A31 monolayer cultures. This is the first report of the paracrine and mitogenic actions of AMF and the results presented here show that AMF functions as a growth factor and suggest a possible role for its activity in normal tissue regeneration and tumor cell dissemination.
Silletti
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Metastasis Research Program, Michigan Cancer Foundation, Detroit, MI 48201.
Raz
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A
D016475:3T3 Cells; D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005354:Fibrosarcoma; D005956:Glucose-6-Phosphate Isomerase / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006133:Growth Substances / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008546:Melanoma, Experimental; D051379:Mice; D060285:Receptors, Autocrine Motility Factor; D011956:Receptors, Cell Surface / Q000378:metabolism; D018121:Receptors, Cytokine; D013997:Time Factors; D014407:Tumor Cells, Cultured; D044767:Ubiquitin-Protein Ligases
D006133:Growth Substances; D011956:Receptors, Cell Surface; D018121:Receptors, Cytokine; D004247:DNA; C555000:AMFR protein, human; C555002:Amfr protein, mouse; D060285:Receptors, Autocrine Motility Factor; D044767:Ubiquitin-Protein Ligases; D005956:Glucose-6-Phosphate Isomerase