Murine leukemia virus infection in immunocompetent adult mice.
The course of infection of a chronic murine leukemia virus (MuLV), E-55+, which induces thymic lymphoma/leukemia in 100% of mice infected as adults, was followed after inoculation of virus into adult mice which generate both cytotoxic T cell and neutralizing antibody responses (Blank 1976). DNA from the tissues of these infected mice was analyzed using a polymerase chain reaction assay to detect integrated provirus. Infection in adult immunocompetent BALB.K mice results in an acute phase characterized by a high number of cells containing provirus DNA followed by a period in which provirus DNA in most tissues reaches background levels until the development of thymic lymphoma/leukemia at 4-7 months. This pattern of infection is different from what has been observed in immunosuppressed mice in which levels of virus-infected cells remain high throughout the preleukemic and leukemic phases.
Tumas
K
K
Department of Pathology, University of Pennsylvania, Philadelphia 19104.
Overmoyer
B
B
Clevenger
C V
CV
Blank
K J
KJ
Prystowsky
M B
MB
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015752:Genes, env; D007109:Immunity; D009052:Leukemia Virus, Murine / Q000254:growth & development*; D007942:Leukemia, Experimental / Q000276:immunology / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D016662:Virus Integration
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides