Higher expression of topoisomerase II in lung cancers than normal lung tissues: different expression pattern from topoisomerase I.
To examine the expression of topoisomerase I and topoisomerase II in primary lung cancer specimens at mRNA level, we carried out Northern blot analysis. As for topoisomerase I expression, there was no remarkable difference between lung cancer specimens and non-cancerous lung tissues. On the other hand, we could detect topoisomerase II mRNA in almost all lung cancer specimens, but not in non-cancerous tissues. By Southern blot analysis, we could not detect large deletion nor rearrangement in DNA level. These results suggest that the expression of topoisomerase II is highly increased in lung cancer at mRNA level and drugs against topoisomerase II might be more tumor-specific than those against topoisomerase I.
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
T
T
Hasegawa
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K
Isobe
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I
Nakashima
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K
Shimokata
D000230:Adenocarcinoma / Q000201:enzymology; D015139:Blotting, Southern; D002277:Carcinoma / Q000201:enzymology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology; D015342:DNA Probes; D004264:DNA Topoisomerases, Type I / Q000096:biosynthesis*; D004250:DNA Topoisomerases, Type II / Q000096:biosynthesis*; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000201:enzymology*; D008175:Lung Neoplasms / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D012016:Reference Values
D015342:DNA Probes; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II