Disruption and fractionation of biological materials by matrix solid-phase dispersion.
The isolation of drug residues, environmental contaminants or naturally occurring component molecules from biological materials is often a complex undertaking. We report here the development and application of a simple approach to the disruption of biological samples that also allows for the rapid fractionation and isolation of the sample's natural components or incurred residues. This process, called matrix solid-phase dispersion (MSPD), combines the use of mechanical forces generated from the grinding of samples with irregular shaped particles (silica or polymer based solid supports) with the lipid solubilizing capacity of a support-bound polymer (octadecylsilyl or others) to produce a sample column material from which dispersed sample matrix components can be selectively isolated. The factors governing this process and examples of its various applications are presented.
Department of Physiology, Pharmacology and Toxicology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
S A
SA
Barker
A R
AR
Long
M E
ME
Hines
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D004785:Environmental Pollutants / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008892:Milk / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D010575:Pesticides / Q000032:analysis; D012822:Silicon Dioxide / Q000737:chemistry
D004785:Environmental Pollutants; D010575:Pesticides; D012822:Silicon Dioxide