Characterisation of LTR sequences involved in the protoplast specific expression of the tobacco Tnt1 retrotransposon.
The tobacco Tnt1 retrotransposon is the only plant retrotransposon that has been shown to be transcriptionally active, and its transcription is strongly induced when preparing leaf-derived protoplasts. We have analysed in this paper the LTR sequences important for Tnt1 expression in tobacco protoplasts. We show that LTR sequences upstream of the TATA box are sufficient to confer protoplast-dependent induction to a heterologous promoter. We also show that this region contains two short activator elements, and that one of these sequences, BII, interacts with protoplast-specific nuclear factors.
Laboratoire de Biologie Cellulaire, INRA, Centre de Versailles, France.
J M
JM
Casacuberta
M A
MA
Grandbastien
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D016385:TATA Box; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D009687:Nuclear Proteins; D004247:DNA