[Observation of live, attenuated hepatitis A vaccines in humans].
Hepatitis A viruses L-A-1 and MBB strains were attenuated by serial passages in laboratory, then appropriately attenuated strains were screened based on marmosets experiments. Three live attenuated vaccine lots L-A-1/P14, L-A-1/P21, MBB/P13 (35 degrees C) were prepared respectively. Three vaccine lots titer were 10(5.5), 10(5.0), 10(4.5) TCID50/ml. Each vaccine lot was inoculated into 2 adults and 8 children 1.0 ml intramuscularly in the upper arm. All volunteers were closely observed for 4 weeks after the inoculation: No one showed local or systemic side-effects and serum ALT, ICD were normal. Every body developed anti-HAV 2-3 weeks postinoculation. Anti-HAV IgM also seroconverted. Neutralizing antibody was detected in serum 4-6 months after inoculation. Three vaccine lots showed no significant difference in safety and immunogenicity. The experimental data showed that the live vaccines were safe and had good immunogenicity.
Shanghai Hygiene and Anti-epidemic Center.
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D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006506:Hepatitis A / Q000517:prevention & control*; D006508:Hepatitis Antibodies / Q000097:blood; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D008297:Male; D014611:Vaccination; D014613:Vaccines, Attenuated; D014761:Viral Hepatitis Vaccines / Q000276:immunology
D006508:Hepatitis Antibodies; D014613:Vaccines, Attenuated; D014761:Viral Hepatitis Vaccines