pH method for determination of cholinesterase in whole blood: collaborative study.
A collaborative study of an indirect method for determination of cholinesterase activity was conducted with cattle blood. Ten collaborators analyzed 8 samples of varying degrees of cholinesterase inhibition. The sample is diluted with a buffer, and the pH change that results from the hydrolysis of acetylcholine is monitored. Repeatability relative standard deviations (RSDr) ranged from 6.6 to 8.6%, and reproducibility relative standard deviations (RSDR) ranged from 10.7 to 17.3% for mean activities of 0.60-0.40 delta pH/h. All samples that were completely inhibited were detected. The method was adopted first action by AOAC International for determination of cholinesterase activity in whole bovine blood.
Iowa State University, Veterinary Diagnostic Laboratory, Ames 50010.
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Imerman
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood; D002417:Cattle; D002802:Cholinesterases / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D015203:Reproducibility of Results
D007202:Indicators and Reagents; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D000109:Acetylcholine