Detection of cytomegalovirus antibodies in serum using the TranSTAT-CMV and CMV Scan assays.
Two hundred serum samples were tested with two assays for rapid detection of cytomegalovirus (CMV) antibodies. The assays were CMV Scan, a two-step latex particle agglutination assay for CMV IgG and IgM antibodies, and TranSTAT-CMV, a newly available six-step solid-phase enzyme immunoassay for CMV IgG antibodies. Comparison of initial results to reference results revealed a sensitivity/specificity of 97.2%/96.8% for CMV Scan and 94.3%/83.0% for TranSTAT-CMV. The same comparison after testing discrepant samples in duplicate increased the sensitivity/specificity to 99.1/100% for CMV Scan and 100%/93.6% for TranSTAT-CMV. Borderline-positive results were observed much more frequently with TranSTAT-CMV than CMV Scan (35 vs. 2), compromising easy interpretation of results. CMV Scan is superior to TranSTAT-CMV on the basis of performance characteristics, simplicity of test performance, and ease of interpretation of results.
Roseff
S D
SD
Department of Pathology, George Washington University Medical Center, Washington, DC.
Campos
J M
JM
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D007074:Immunoglobulin G / Q000032:analysis; D007223:Infant; D007231:Infant, Newborn; D007841:Latex Fixation Tests / Q000379:methods / Q000592:standards; D008297:Male; D008875:Middle Aged; D013223:Statistics as Topic
D000914:Antibodies, Viral; D007074:Immunoglobulin G