Development of congenic strains of mice carrying amyloidogenic apolipoprotein A-II (Apoa2c). Apoa2c reduces the plasma level and the size of high density lipoprotein.
A congenic strain of mice with amyloidogenic apolipoprotein A-II (Apoa2c) on the genetic background of the amyloidosis-resistant SAM-R/1 strain was produced by 12 generations of backcrossing. Genome mapping using endogenous murine leukemia proviral markers was done in the congenic strain, termed R1.P1-Apoa2c. We confirmed that only a small region surrounding the apoA-II gene on chromosome 1 was transferred from the genome of the donor SAM-P/1 strain. The level and particle size of plasma high density lipoprotein were decreased in R1.P1-Apoa2c mice compared to those in the progenitor SAM-R/1 mice. The function of apoA-II can be studied using this strain of mice.
Higuchi
K
K
Department of Senescence Biology, Kyoto University, Japan.
Kitado
H
H
Kitagawa
K
K
Kogishi
K
K
Naiki
H
H
Takeda
T
T
D000686:Amyloidosis / Q000235:genetics; D000818:Animals; D016633:Apolipoprotein A-II / Q000031:analogs & derivatives* / Q000235:genetics / Q000502:physiology; D002874:Chromosome Mapping / Q000662:veterinary; D003433:Crosses, Genetic; D005260:Female; D005819:Genetic Markers; D009052:Leukemia Virus, Murine / Q000235:genetics; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000097:blood / Q000235:genetics*; D011533:Proviruses / Q000235:genetics
D016633:Apolipoprotein A-II; D005819:Genetic Markers; D008075:Lipoproteins, HDL; C079456:amyloidogenic apolipoprotein A-II, mouse