Immunohistochemical detection of swine influenza A virus in formalin-fixed and paraffin-embedded tissues.
An avidin-biotin complex (ABC) immunohistochemical method utilizing a commercially-available polyclonal antiserum to human influenza A virus was used to detect antigens of influenza A virus in formalin-fixed, paraffin-embedded tissues of swine. Influenza A antigens were immunohistochemically detected in 28/30 cases in which influenza A virus was demonstrated by virus isolation and in 5/22 cases suspected to be influenza A-infected by clinical and histological criteria, but from which the virus was not isolated. Viral antigen was not demonstrated in 30/30 cases not suspected clinically or histologically to be associated with influenza infection. This method is a convenient, sensitive, and specific means of influenza A virus detection and is applicable to both routine diagnosis of influenza A virus infection and to retrospective and prospective studies of the occurrence and the pathogenesis of this virus in pigs.
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
D M
DM
Haines
E H
EH
Waters
E G
EG
Clark
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D008168:Lung / Q000382:microbiology; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
D000956:Antigens, Viral; D007106:Immune Sera; D001360:Avidin; D001710:Biotin