[Delayed hypersensitivity and the primary antibody reaction after administration of infectious bovine rhinotracheitis inactivated oil vaccine to calves premedicated with glucan].
The level of delayed skin hypersensitivity (DSH) to DNFB and of the primary immune reaction was evaluated in the calves immunized with an inactivated oil IBR vaccine (V group) and in the calves premedicated with glucan (seven days before vaccine administration) and subsequently immunized with the mentioned vaccine (GV group). The DSH test did not reveal an alteration of cellular immunological reactivity in the calves immunized with an inactivated oil IBR vaccine; after the vaccine administration there was no significant difference in the DSH level from the value before vaccine administration nor in comparison with the value of control calves (K group), Tab. I. But in the immunized calves which were glucan-premedicated (from Pleurotus ostreatus; 10 mg/kg l.w.) a significantly higher DSH level was determined not only in comparison with the initial value (P < 0.05) before glucan administration but also with the value of control calves (P < 0.05), Tab. I. The categorization of calves according to the DSH level also points to the immunomodulating effect of glucan in this sense (Fig. 1); while the value of skin test ranged from 3.6 to 6.5 mm in the highest percentage of the calves of V and K groups on the dates before and after administration of the mentioned preparations, a marked increase (on the date after administration) in the number of calves (from 22 to 67%) with the value of skin test higher than 6.5 mm was observed in the calves of GV group. The vaccine administration in itself (V group) did not induce the production of measurable amounts of serum IBR antibodies till day 14 after immunization.(ABSTRACT TRUNCATED AT 250 WORDS)
Univerzita veterinárskeho lekárstva, Kosice.
S
S
Paulík
V
V
Bajová
G
G
Benko
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002417:Cattle / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology; D005936:Glucans / Q000008:administration & dosage*; D006968:Hypersensitivity, Delayed / Q000662:veterinary*; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology*; D011124:Polyporaceae; D012882:Skin Tests / Q000662:veterinary; D015164:Vaccines, Inactivated; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D005936:Glucans; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D004139:Dinitrofluorobenzene