Nucleotide and predicted amino acid sequence of a new member of the ras gene family from the slime mold Physarum polycephalum.
A second ras homologue, designated Ppras2, has been isolated from Physarum polycephalum mixed amoebae and flagellates cDNA library. Ppras2 encodes a protein of 193 amino acids of a calculated M(r) of 21,633. The deduced amino acid sequence is highly homologous to Ppras1 and other ras genes from slime molds. The amino acid sequence at the C-terminus of the putative protein suggests that like other slime mold ras proteins but not the ones from other organisms, it is modified by geranylgeranylation rather than farnesylation, it is unpalmitoylated and contains a putative lysine-rich domain.
Institute of Biochemistry, Warsaw University, Poland.
P
P
Kozlowski
Z
Z
Tymowska
K
K
Toczko
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D005800:Genes, Fungal; D011905:Genes, ras; D008969:Molecular Sequence Data; D016839:Physarum polycephalum / Q000235:genetics*
D004247:DNA