title: Detection of HIV in human vitreous.
abstract: Assay of human vitreous specimens obtained postmortem for HIV antibodies, or HIV p24 antigen, is reported to be a reliable technique to demonstrate HIV infection in possible cornea donors from whom serum could not be obtained. We tested three vitreous samples obtained during vitrectomy from two HIV-positive patients. One patient exhibited the clinical AIDS syndrome. HIV antigen and antibody tests were negative in all specimens. HIV proviral DNA was detected by PCR only in the vitreous of the patient with AIDS. Therefore, testing only vitreous samples is insufficient to exclude HIV infection in potential cornea donors.
authors.affiliation: Department of Ophthalmology, University of Cologne, Köln, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Mietz
authors.forename: K
authors.initials: K
authors.lastname: Heimann
authors.forename: J
authors.initials: J
authors.lastname: Kühn
authors.forename: U
authors.initials: U
authors.lastname: Wieland
authors.forename: H J
authors.initials: HJ
authors.lastname: Eggers
mesh_terms: D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D015828:Eye Infections, Viral / Q000382:microbiology*; D006678:HIV / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D015483:HIV Antibodies / Q000032:analysis; D016655:HIV Core Protein p24 / Q000032:analysis; D015658:HIV Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011533:Proviruses / Q000235:genetics / Q000276:immunology; D012163:Retinal Detachment / Q000601:surgery; D014821:Vitrectomy; D014822:Vitreous Body / Q000276:immunology / Q000382:microbiology*
chemical_list: D004279:DNA, Viral; D015483:HIV Antibodies; D016655:HIV Core Protein p24