title: Cloning, sequence and expression of the gene encoding the malolactic enzyme from Lactococcus lactis.
abstract: Many lactic acid bacteria can carry out malolactic fermentation. This secondary fermentation is mediated by the NAD- and Mn(2+)-dependent malolactic enzyme, which catalyses the decarboxylation of L-malate to L-lactate. The gene we call mleS, coding for malolactic enzyme, was isolated from Lactococcus lactis. The mleS gene consists of one open reading frame capable of coding for a protein with a calculated molecular mass of 59 kDa. The amino acid sequence of the predicted MleS gene product is homologous to the sequences of different malic enzymes. Bacterial and yeast cells expressing the malolactic gene convert L-malate to L-lactate.
authors.affiliation: Laboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne, INRA, Montpellier, France.
authors.forename: V
authors.initials: V
authors.lastname: Ansanay
authors.forename: S
authors.initials: S
authors.lastname: Dequin
authors.forename: B
authors.initials: B
authors.lastname: Blondin
authors.forename: P
authors.initials: P
authors.lastname: Barre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli; D005798:Genes, Bacterial; D007106:Immune Sera; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D008291:Malate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051381:Rats; D012441:Saccharomyces cerevisiae; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D007106:Immune Sera; C037731:malolactic enzyme; D008291:Malate Dehydrogenase