title: Mass spectrometric evidence for a disulfide bond in aequorin regeneration.
abstract: Tryptic digests of purified recombinant apoaequorin were analyzed, before and after reduction with DTT, by fast atom bombardment mass spectrometry. The results showed that apoaequorin contains a disulfide bond between Cys145 and Cys152 and that the reduction of this bond is involved in the regeneration of aequorin.
authors.affiliation: PRESTO, JRDC, Solar Energy Research Group, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohmiya
authors.forename: S
authors.initials: S
authors.lastname: Kurono
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
authors.forename: T F
authors.initials: TF
authors.lastname: Fagan
authors.forename: F I
authors.initials: FI
authors.lastname: Tsuji
mesh_terms: D000331:Aequorin / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D004220:Disulfides / Q000032:analysis*; D004229:Dithiothreitol / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D037401:Scyphozoa; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D014357:Trypsin
chemical_list: D004220:Disulfides; D010446:Peptide Fragments; D011994:Recombinant Proteins; D000331:Aequorin; D014357:Trypsin; D004229:Dithiothreitol