Evidence of new antigens in the mouse cumulus oophorus during preovulatory cumulus expansion.
The effects of an antiserum (anti-COC) against ovulated mouse cumulus-oocyte complexes (COC) on in vitro fertilization of mouse oocytes were studied. Preincubation of ovulated COC with various concentrations of anti-COC led to dose-dependent impairment of fertilization rates as well as to a decrease in the number of spermatozoa attached to the zona pellucida. Anti-COC was used to probe Western blots of cumulus proteins. These cumuli were obtained from 2 experimental groups of mice corresponding to 2 different maturational stages (preovulatory immature COC or preovulatory mature COC). Two antigens (70 and 80 kDa) present in cumulus intercellular matrix from mature COC were only found as traces in matrix from immature COC. In addition, the protein pattern of the cumulus intercellular matrix was different from that of cumulus cells, whatever the COC maturational stage. These results indicate the appearance of new proteins in the cumulus oophorus during preovulatory expansion and are consistent with the contraceptive action of anti-COC.
Amiel
M L
ML
Institute National de la Santé et de la Recherche Médicale (INSERM), Clamart, France.
Moos
J
J
Tesarik
J
J
Testart
J
J
D000818:Animals; D000906:Antibodies; D000941:Antigens; D003269:Contraception, Immunologic; D005109:Extracellular Matrix / Q000276:immunology; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000276:immunology*; D051379:Mice; D009865:Oocytes / Q000276:immunology; D006080:Ovarian Follicle / Q000166:cytology / Q000276:immunology
D000906:Antibodies; D000941:Antigens