Calpain-calpastatin system of canine basilar artery in vasospasm.
Vasospasm was produced in the canine basilar artery by a two-hemorrhage method, while contraction was induced in the normal canine basilar artery by a local application of KCl or serotonin after transclival exposure. The control animals were injected with saline instead of fresh blood. The activation of mu-calpain, a Ca(++)-dependent neutral protease, in the basilar artery was studied by evaluating the conversion from its inactivated into its activated form on immunoblots. In addition, the activity of calpastatin, an intrinsic inhibitor of calpain, in the basilar artery was determined by assay. The majority of the mu-calpain was inactivated in the control group. In the spastic group, mu-calpain was generally activated markedly in the early stage of vasospasm and moderately thereafter. The contraction induced by KCl or serotonin application was classified into the early phasic and the later tonic stages; mu-calpain was usually activated in the phasic stage and inactivated in the tonic stage. Calpastatin activity was significantly decreased during vasospasm, whereas it was not significantly changed in KCl- or serotonin-induced contraction. The final activity of mu-calpain results from the balance of mu-calpain and calpastatin. This suggests that mu-calpain activity was enhanced continuously in the spastic group and transiently in the KCl or serotonin group, and that the continuous activation of mu-calpain during vasospasm probably induced more proteolytic changes compared to those in the KCl or serotonin group.
Yamaura
I
I
Department of Neurosurgery, Hyogo College of Medicine, Japan.
Tani
E
E
Saido
T C
TC
Suzuki
K
K
Minami
N
N
Maeda
Y
Y
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism* / Q000502:physiology; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004285:Dogs; D015151:Immunoblotting; D066298:In Vitro Techniques; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D012701:Serotonin; D011189:Potassium Chloride; C033668:calpastatin; D002154:Calpain