title: Analysis of a cloned Francisella tularensis outer membrane protein gene and expression in attenuated Salmonella typhimurium.
abstract: We have determined the nucleotide sequence of fopA from Francisella tularensis. Using the polymerase chain reaction fopA was detected in high and low virulence biotypes of F. tularensis. fopA was stably maintained in pBluescript in attenuated Salmonella typhimurium where FopA was expressed and located in the outer membrane. This recombinant will be suitable for studies on the role of FopA in immunity against tularaemia.
authors.affiliation: Chemical and Biological Defence Establishment, Salisbury, Wiltshire, UK.
authors.forename: D L
authors.initials: DL
authors.lastname: Leslie
authors.forename: J
authors.initials: J
authors.lastname: Cox
authors.forename: M
authors.initials: M
authors.lastname: Lee
authors.forename: R W
authors.initials: RW
authors.lastname: Titball
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005604:Francisella tularensis / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology; D014406:Tularemia / Q000276:immunology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; C083436:FopA protein, Francisella tularensis