title: Bacteremia due to Rochalimaea henselae in a child: practical identification of isolates in the clinical laboratory.
abstract: Two closely related species of Rochalimaea, Rochalimaea quintana and Rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. We report a case of culture-proven R. henselae bacteremia in a child with persistent fever. Serologic evidence of infection by R. henselae was ascertained by testing sera at two intervals for immunoglobulin G or immunoglobulin M antibodies by enzyme immunoassay and immunoblot. The case isolate and a collection of other strains (R. henselae, R. quintana, and related organisms) were used to test commercial identification systems for their comparative utility in the identification of Rochalimaea spp. on a practical basis. Of six systems designed for testing of either fastidious or anaerobic isolates of bacteria, the MicroScan Rapid Anaerobe Panel was the only system that distinguished R. henselae from R. quintana. Four of five others gave reactions that were unique within their data bases but did not distinguish Rochalimaea isolates at the species level.
authors.affiliation: Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
authors.forename: D F
authors.initials: DF
authors.lastname: Welch
authors.forename: D M
authors.initials: DM
authors.lastname: Hensel
authors.forename: D A
authors.initials: DA
authors.lastname: Pickett
authors.forename: V H
authors.initials: VH
authors.lastname: San Joaquin
authors.forename: A
authors.initials: A
authors.lastname: Robinson
authors.forename: L N
authors.initials: LN
authors.lastname: Slater
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D002648:Child; D006801:Humans; D008297:Male; D012288:Rickettsiaceae Infections / Q000175:diagnosis / Q000382:microbiology*; D016996:Rickettsieae / Q000302:isolation & purification*