Molecular cloning and characterization of PstI fragments of bovine herpesvirus-1 DNA.
The DNA from an Indian isolate of BHV-1 was cleaved with the restriction enzyme PstI and cloned into pUC9 plasmid. Two recombinant plasmids were then analyzed using restriction endonucleases PstI, EcoRI, BamHI, SmaI, SalI, HindIII, BglI, HaeIII, XhoI, RsaI and StuI. On the basis of digested fragments of recombinant plasmids, two plasmids could be mapped for restriction enzymes SalI, StuI, SmaI and XhoI using single and double digestion.
Gupta
P K
PK
National Biotechnology Center, Indian Veterinary Research Institute, Izatnagar.
Rai
A
A
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D005822:Genetic Vectors; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D007668:Kidney; D010957:Plasmids
D004274:DNA, Recombinant; D004279:DNA, Viral; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific