title: Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.
abstract: The effects of 30 type 1 and of 2 (ricin and volkensin) type 2 ribosome-inactivating proteins (RIPs) on Escherichia coli and Agrobacterium tumefaciens cell-free translation systems were compared with the effects on a rabbit reticulocyte translation system. The depurinating activity of RIPs on E. coli ribosomes was also evaluated. Only six type 1 RIPs inhibited endogenous mRNA-directed translational activity of E. coli lysates, with submicromolar 50% inhibitory concentrations. Four RIPs had similar activities on poly(U)-directed phenylalanine polymerization by E. coli ribosomes, and three RIPs inhibited poly(U)-directed polyphenylalanine synthesis by A. tumefaciens ribosomes, with submicromolar 50% inhibitory concentrations.
authors.affiliation: Departmento de Bioquimica, Biologia Molecular y Fisiologia, Facultad de Ciencias, Universidad de Valladolid, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Girbés
authors.forename: L
authors.initials: L
authors.lastname: Barbieri
authors.forename: M
authors.initials: M
authors.lastname: Ferreras
authors.forename: F J
authors.initials: FJ
authors.lastname: Arias
authors.forename: M A
authors.initials: MA
authors.lastname: Rojo
authors.forename: R
authors.initials: R
authors.lastname: Iglesias
authors.forename: C
authors.initials: C
authors.lastname: Alegre
authors.forename: C
authors.initials: C
authors.lastname: Escarmis
authors.forename: F
authors.initials: F
authors.lastname: Stirpe
mesh_terms: D016960:Agrobacterium tumefaciens / Q000187:drug effects* / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012335:RNA, Ribosomal / Q000737:chemistry; D012338:RNA, Ribosomal, 23S / Q000737:chemistry; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects*; D013045:Species Specificity
chemical_list: D010940:Plant Proteins; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; C065096:RNA, ribosomal, 26S; D054789:Ribosome Inactivating Proteins, Type 1; C012857:crotin II protein, Croton tiglium; D006026:Glycoside Hydrolases