Gluten exorphin C. A novel opioid peptide derived from wheat gluten.
A novel opioid peptide, Tyr-Pro-Ile-Ser-Leu, was isolated from the pepsin-trypsin-chymotrypsin digest of wheat gluten. Its IC50 values were 40 microM and 13.5 microM in the GPI and MVD assays, respectively. This peptide was named gluten exorphin C. Gluten exorphin C had a structure quite different from any of the endogenous and exogenous opioid peptides ever reported in that the N terminal Tyr was the only aromatic amino acid. The analogs containing Tyr-Pro-X-Ser-Leu were synthesized to study its structure-activity relationship. Peptides in which X was an aromatic amino acid or an aliphatic hydrophobic amino acid had opioid activity.
Fukudome
S
S
Research Control Department, Nisshin Flour Milling Co., Ltd., Nihonbashi, Tokyo, Japan.
Yoshikawa
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M
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D005983:Glutens / Q000737:chemistry*; D008969:Molecular Sequence Data; D009294:Narcotics / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D014908:Triticum / Q000737:chemistry*
D009294:Narcotics; D010455:Peptides; C019178:exorphins; D005983:Glutens