Participation of cathepsin B in processing of antigen presentation to MHC class II.
Cellular and humoral immune responses to vaccines of hepatitis B type and rabies were inhibited by specific inhibitors of cathepsin B, specific synthetic substrates of cathepsin B and anti-cathepsin B antibody. Therefore the lysosomal cathepsin B of antigen presenting cells plays an essential role in processing of these antigens for presentation to MHC class II. One of the active sites of cathepsin B, VN217-222 shares highly homologous sequences with a part of the desetope, a binding domain of antigenic peptides, VN57-62 of MHC class II, beta-chain. This evidence suggests that the peptides processed by the substrate specificity of cathepsin B exhibit a common affinity to bind with the desetope of MHC class II, beta-chain.
Matsunaga
Y
Y
Department of The Third Internal Medicine, School of Medicine, University of Tokushima, Japan.
Saibara
T
T
Kido
H
H
Katunuma
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000201:enzymology*; D000941:Antigens / Q000378:metabolism*; D002401:Cathepsin B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002999:Clone Cells; D006510:Hepatitis B Antibodies / Q000096:biosynthesis; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011819:Rabies Vaccines / Q000737:chemistry / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D011819:Rabies Vaccines; D011948:Receptors, Antigen, T-Cell; D002401:Cathepsin B