Enzyme-linked immunosorbent assay for detection of human immunoglobulin G to lipopolysaccharide of spotted fever group rickettsiae.
An enzyme-linked immunosorbent assay for detecting human immunoglobulin G to spotted fever group rickettsiae was developed and tested. The assay uses proteinase K-resistant material, characteristic of the rickettsial lipopolysaccharides shown to be group specific by immunoblots, as the antigen. The results indicate that the assay provides a sensitive, yet specific, alternative method for diagnosing rickettsial diseases.
Jones
D
D
Viral and Rickettsial Zoonoses Branch, National Center for Infectious Diseases, Atlanta, Georgia 30333.
Anderson
B
B
Olson
J
J
Greene
C
C
D019286:Endopeptidase K; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology; D012281:Rickettsia / Q000276:immunology* / Q000302:isolation & purification; D012282:Rickettsia Infections / Q000175:diagnosis* / Q000276:immunology; D012697:Serine Endopeptidases / Q000276:immunology
D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D012697:Serine Endopeptidases; D019286:Endopeptidase K