Antibody responses in the serum and respiratory tract of mice following oral vaccination with liposomes coated with filamentous hemagglutinin and pertussis toxoid.
Mice were orally vaccinated with liposomes coated with filamentous hemagglutinin (FHA) and detoxified pertussis toxin (PT) of Bordetella pertussis. FHA- and PT-specific immunoglobulin G (IgG) was detected in serum, and both IgG and IgA were detected in lung washes following the immunization. Antibody responses in mice immunized with liposomes coated with FHA and PT were significantly higher than those in mice immunized with free FHA and PT, which demonstrated the adjuvanticity of the liposome carrier. The results indicate the potential usefulness of this approach for eliciting immune responses against FHA and PT (and perhaps other pertussis antigens) in humans and its possible utility in large-scale vaccination to protect against both B. pertussis infection and disease.
Guzman
C A
CA
Institute of Microbiology, University of Genoa, Italy.
Molinari
G
G
Fountain
M W
MW
Rohde
M
M
Timmis
K N
KN
Walker
M J
MJ
D018829:Adhesins, Bacterial; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D015153:Blotting, Western; D004337:Drug Carriers; D005260:Female; D006388:Hemagglutinins / Q000008:administration & dosage* / Q000276:immunology; D008081:Liposomes; D008168:Lung / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D014121:Toxoids / Q000008:administration & dosage* / Q000276:immunology; D014611:Vaccination; D010566:Virulence Factors, Bordetella
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D004337:Drug Carriers; D006388:Hemagglutinins; D008081:Liposomes; D014121:Toxoids; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; C051093:pertussis toxoid