title: Specific in vitro labeling of cells with a fluorine-19 probe encapsulated in antibody-targeted liposomes: a F-19 NMR spectroscopy study.
abstract: Liposomes containing dexamethasone phosphate (DMp) were covalently coupled to protein A and then incubated with murine L929 fibroblast and RDM4 thymoma cells in the presence of monoclonal antibodies specific for the major histocompatibility complex. The detection of the specific F-19 labeling of cells is rapid (minutes). Such a strategy might be useful to study the kinetics of internalization processes of bound liposomes on cultured living cells.
authors.affiliation: Centre de Résonance Magnétique Biologique et Médicale, (URA CNRS 1186), Faculté de Médecine, Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Vion-Dury
authors.forename: P
authors.initials: P
authors.lastname: Machy
authors.forename: S
authors.initials: S
authors.lastname: Confort-Gouny
authors.forename: L
authors.initials: L
authors.lastname: Leserman
authors.forename: P J
authors.initials: PJ
authors.lastname: Cozzone
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003907:Dexamethasone; D005461:Fluorine; D066298:In Vitro Techniques; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013205:Staphylococcal Protein A; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D008081:Liposomes; D013205:Staphylococcal Protein A; D005461:Fluorine; D003907:Dexamethasone