Cloning of the BamHI repeat from Amaranthus and study of its methylation in genomic DNA during dedifferentiation.
A 670 bp BamHI repeat was isolated from the genomic DNA of Amaranthus paniculatus and was cloned in pUC19 vector (pABH3). The level of methylation of total genomic DNA and the BamHI repeat therein was studied by employing methylation sensitive enzymes to digest genomic DNA isolated from whole seedlings, hypocotyl, callus, and suspension culture of Amaranthus. The restriction pattern indicated double methylation of the target site CCGG. Methylation pattern of both the total genomic DNA and the cloned repeat was found to be similar during dedifferentiation indicating that there may not be a general relationship between hypomethylation and dedifferentiation.
Pradhan
S
S
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Sharma
A K
AK
Sopory
S K
SK
D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D005822:Genetic Vectors; D016678:Genome; D008745:Methylation; D010945:Plants, Edible / Q000166:cytology / Q000235:genetics*; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid
D004274:DNA, Recombinant; D015245:Deoxyribonuclease BamHI