title: Sequences and expression of the porcine apolipoprotein A-I and C-III mRNAs.
abstract: Apolipoprotein A-I (ApoA-I) is the principal protein component of plasma high-density lipoprotein (HDL) and an activator of lecithin:cholesterol acyltransferase. Apolipoprotein C-III (ApoC-III) exchanges between triglyceride-rich lipoproteins and HDL and inhibits the lipolysis and uptake of triglyceride-rich lipoproteins. To study the expression of these Apo-encoding genes in the developing swine, apoA-I and apoC-III cDNAs from a lambda gt11 porcine liver cDNA library and apoC-III from a porcine genomic DNA library were isolated and sequenced. The predicted amino acid (aa) sequence and composition for ApoC-III matched the N-terminal aa sequence and composition of purified swine ApoC-III. Comparison among known ApoA-I and C-III aa sequences from various species revealed strict conservation of amphipathic helices. In adult pigs, the apoA-I mRNA was found predominantly in the intestine and liver, with a small amount detected in the testes. In contrast, apoC-III mRNA was found predominantly in adult liver. Developmentally, hepatic apoA-I and apoC-III mRNAs were expressed in livers of fetal, newborn, and suckling animals. Intestinal apoA-I and apoC-III mRNAs, however, were detected only in postpartum animals. Although intestinal apoA-I mRNA expression continued into the adult, intestinal apoC-III mRNA expression declined sharply after the newborn period.
authors.affiliation: Department of Pediatrics, University of Chicago, Pritzker School of Medicine, IL 60637.
authors.forename: V N
authors.initials: VN
authors.lastname: Trieu
authors.forename: J
authors.initials: J
authors.lastname: Hasler-Rapacz
authors.forename: J
authors.initials: J
authors.lastname: Rapacz
authors.forename: D D
authors.initials: DD
authors.lastname: Black
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000096:biosynthesis / Q000235:genetics*; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005333:Fetus / Q000378:metabolism; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000196:embryology; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D013552:Swine
chemical_list: D016632:Apolipoprotein A-I; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C