title: YAC clone contigs surrounding the Zfx and Pola loci on the mouse X chromosome.
abstract: Pulsed-field mapping of a number of DNA markers in the Pola-Zfx region of the mouse X chromosome has established a genomic restriction map extending over 1.4 Mb. A number of YAC clones from the Pola-Zfx region have been isolated from three mouse YAC libraries--first, a mouse C57BL/10 partial R1 YAC library constructed in a yeast strain carrying a rad52 mutation (Chartier et al. (1992) Nature Genetics 1: 132-136); second, a mouse C3H partial R1 library (Larin et al. (1991) Proc. Natl. Acad. Sci. USA 88: 4123-4127); and third, a mouse C57BL/6 partial R1 library (Burke et al. (1991) Mamm. Genome 1:65). Six YAC clones encompass the Zfx-Pola region, confirming the linkage of the Pola and Zfx loci and establishing a physical map order in this region of cen-Pola-DXCrc140-DXCrc57-Zfx-tel. The close linkage of Pola and Zfx in the mouse genome suggests that the POLA and ZFX loci must also be closely linked on the human X chromosome.
authors.affiliation: Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, United Kingdom.
authors.forename: R M
authors.initials: RM
authors.lastname: Hamvas
authors.forename: Z
authors.initials: Z
authors.lastname: Larin
authors.forename: N
authors.initials: N
authors.lastname: Brockdorff
authors.forename: S
authors.initials: S
authors.lastname: Rastan
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: F L
authors.initials: FL
authors.lastname: Chartier
authors.forename: S D
authors.initials: SD
authors.lastname: Brown
mesh_terms: D000818:Animals; D001483:Base Sequence; D016386:Chromosome Walking; D015825:Chromosomes, Fungal; D004268:DNA-Binding Proteins / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D015723:Gene Library; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008297:Male; D051379:Mice / Q000235:genetics*; D008809:Mice, Inbred C3H / Q000235:genetics; D008810:Mice, Inbred C57BL / Q000235:genetics; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014157:Transcription Factors; D014960:X Chromosome
chemical_list: D004268:DNA-Binding Proteins; D005819:Genetic Markers; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C062076:zinc finger protein, X-linked