Presence of carnitine acetyltransferase in peroxisomes and in mitochondria of oleic acid-grown Saccharomyces cerevisiae.
Activity of carnitine acetyltransferase was detected in glucose- and oleic acid-grown Saccharomyces cerevisiae. Oleic acid-grown cells showed a ten-fold higher activity than glucose-grown cells. Subcellular fractionation of oleic acid-grown cells showed that carnitine acetyltransferase was present in peroxisomes, mitochondria, and cytosol. The results suggested the plausible presence of an 'acetylcarnitine shuttle' in this yeast, as in the case of an n-alkane-assimilating yeast, Candida tropicalis.
Atomi
H
H
Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Japan.
Ueda
M
M
Suzuki
J
J
Kamada
Y
Y
Tanaka
A
A
D000105:Acetyl Coenzyme A / Q000378:metabolism; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology; D005947:Glucose / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D009829:Oleic Acids; D019301:Oleic Acid; D000105:Acetyl Coenzyme A; D002332:Carnitine O-Acetyltransferase; D005947:Glucose