Procoagulant activities expressed by peripheral blood mononuclear cells.
These combined data support the concept that the procoagulant response elicited by mononuclear cells, particularly monocytes, is accomplished through regulated binding site-mediated (or perhaps "receptor"-mediated) assembly of proteolytic activities at their membrane surface. Because the work of several laboratories indicate that the monocytes provide the appropriate membrane surface for the assembly and function of all the coagulation complexes required for thrombin production in vivo, monocytes may provide a unique opportunity to investigate how coagulant reactions are regulated on cell surfaces through both receptor-mediated events as well as by channeling a product of one reaction to serve as a mediator of a second reaction.
Tracy
P B
PB
Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405.
Robinson
R A
RA
Worfolk
L A
LA
Allen
D H
DH
D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004731:Endotoxins / Q000494:pharmacology; D005165:Factor V / Q000378:metabolism*; D005169:Factor VIII / Q000378:metabolism*; D005170:Factor X / Q000378:metabolism*; D015951:Factor Xa; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology; D013925:Thromboplastin / Q000378:metabolism*
D004731:Endotoxins; C022475:prothrombinase complex; D005165:Factor V; D005169:Factor VIII; D005170:Factor X; D013925:Thromboplastin; D015951:Factor Xa