Parallel genome analysis by two-dimensional DNA typing.
By two-dimensional (2-D) DNA typing a restriction enzyme digest of genomic DNA can be resolved on the basis of both size and base-pair sequence and subsequently analysed by repeat probe hybridization to reveal sequence variants at multiple genomic sites in parallel. The system has been partly automated and allows for large-scale comparative analysis of complex genomes in a cost-effective manner.
Mullaart
E
E
Ingeny B. V., Leiden, The Netherlands.
de Vos
G J
GJ
te Meerman
G J
GJ
Uitterlinden
A G
AG
Vijg
J
J
D001331:Automation; D010582:Bacteriophage lambda / Q000235:genetics; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes