title: The effects of aging on the function of alveolar macrophages in mice.
abstract: In order to determine whether the function of alveolar macrophages (AM) is modulated by aging, we measured the TNF-alpha production, phagocytic function, and surface antigen expression of AM from young and old mice. When AM were primed by IFN-gamma (500 units/ml) and triggered by LPS (100 micrograms/ml), TNF-alpha production by AM was significantly smaller in old mice as compared with young mice (young mice: 161.7 +/- 28.2 units/ml; old mice: 89.3 +/- 13.6 units/ml, P < 0.05). The percentage of AM which phagocytosed latex particles (more than one particle) in old mice was significantly lower than in young mice (young: 78.1 +/- 2.5%; old: 62.8 +/- 3.4%, P < 0.05). Ia antigen expression of the AM was significantly higher and asialo-GM1 antigen expression was significantly lower in old mice than in young mice (Ia: young, 0.030 +/- 0.005; old, 0.092 +/- 0.024, P < 0.05; asialo-GM1: young, 0.-9 +/- 0.01; old, 0.75 +/- 0.07, P < 0.01). These results suggest that alveolar macrophage function is at least decreased in part with aging in mice.
authors.affiliation: Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Higashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Ishida
authors.forename: M
authors.initials: M
authors.lastname: Ohata
authors.forename: T
authors.initials: T
authors.lastname: Furuse
authors.forename: C
authors.initials: C
authors.lastname: Shu
authors.forename: S
authors.initials: S
authors.lastname: Teramoto
authors.forename: T
authors.initials: T
authors.lastname: Matsuse
authors.forename: E
authors.initials: E
authors.lastname: Sudo
mesh_terms: D000375:Aging / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D007840:Latex; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008863:Microspheres; D010586:Phagocytes / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000954:Antigens, Surface; D007840:Latex; D014409:Tumor Necrosis Factor-alpha