Block of long-term potentiation by intracellular application of anti-phosphatidylinositol 4,5-bisphosphate antibody in hippocampal pyramidal neurons.
We examined the effects of black widow spider toxin and anti-phosphatidylinositol 4,5-bisphosphate antibody on the changes in excitatory postsynaptic currents and spontaneous excitatory postsynaptic currents accompanying long-term potentiation using whole-cell recording from hippocampal CA1 pyramidal neurons of rodents. In the presence of black widow spider toxin, tetanic stimulation of input fibers produced a short-lived potentiation followed by a gradual decline of the excitatory postsynaptic current amplitude. With an anti-phosphatidylinositol 4,5-bisphosphate antibody containing pipette, tetanus elicited only decremental potentiation of excitatory postsynaptic currents with a reduced frequency of spontaneous excitatory postsynaptic currents, suggesting inhibition of retrograde reinforcement from the antibody-injected neuron. With both black widow spider toxin and anti-phosphatidylinositol 4,5-bisphosphate antibody, neurons showed a rapid depression of excitatory postsynaptic currents after tetanus. The results indicate that time-dependent interactions between presynaptic terminals and the postsynaptic spikes take place during long-term potentiation.
Tsubokawa
H
H
Department of Physiology, Jichi Medical School, Tochigi-ken, Japan.
Robinson
H P
HP
Takenawa
T
T
Kawai
N
N
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D017152:Antibodies, Antiphospholipid / Q000276:immunology / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D004558:Electric Stimulation; D005849:Gerbillinae; D017774:Long-Term Potentiation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates / Q000276:immunology*; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017981:Receptors, Neurotransmitter / Q000187:drug effects; D013111:Spider Venoms / Q000494:pharmacology*; D013746:Tetany / Q000503:physiopathology
D017152:Antibodies, Antiphospholipid; D000911:Antibodies, Monoclonal; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D017981:Receptors, Neurotransmitter; D013111:Spider Venoms; C020763:alpha-latrotoxin