title: Effect of adenosine 5'-triphosphate on secretagogue-stimulated (14C)-aminopyrine accumulation by rabbit isolated gastric glands.
abstract: The effect of adenosine 5'-triphosphate (ATP) on gastric acid secretion stimulated by histamine, carbachol and dibutyryl-cAMP (db-cAMP) was studied using glands isolated from rabbit gastric mucosa. The (14C)-aminopyrine (AP) accumulation method was used as an index of acid production by the gastric glands. Histamine-stimulated AP accumulation was significantly inhibited by ATP (10 mumol/l-1 mmol/l). The inhibitory action of ATP appeared to be specific, inasmuch as this nucleotide had no significant effect on basal secretion or secretion stimulated by carbachol or db-cAMP. The antisecretory effect of ATP on histamine-stimulated glands was not affected by the P1-purinoceptor antagonist, theophylline. Pretreatment of glands with indomethacin, a well known prostaglandin synthesis inhibitor, led to a significant reduction of the inhibitory responses to ATP. These results show that ATP inhibits the histamine-stimulated AP accumulation by rabbit isolated gastric glands and suggest that this effect is not due to an ectoenzymatic conversion of ATP into adenosine but to a direct effect of ATP which may be mediated via a P2-purinoceptor subtype linked to prostaglandin production.
authors.affiliation: Departmento de Fisiología, Facultad de Medicina, Universidad del País Vasco (UPV-EHU), Bilbao, Spain.
authors.forename: C E
authors.initials: CE
authors.lastname: Gil-Rodrigo
authors.forename: R
authors.initials: R
authors.lastname: Gomez
authors.forename: J M
authors.initials: JM
authors.lastname: Gandarias
authors.forename: B
authors.initials: B
authors.lastname: Galdiz
authors.forename: M
authors.initials: M
authors.lastname: Carou
authors.forename: I
authors.initials: I
authors.lastname: Bergaretxe
authors.forename: A
authors.initials: A
authors.lastname: Vallejo
authors.forename: L F
authors.initials: LF
authors.lastname: Ainz
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000632:Aminopyrine / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D010477:Perfusion; D011817:Rabbits; D013806:Theophylline / Q000494:pharmacology
chemical_list: D000632:Aminopyrine; D003994:Bucladesine; D006632:Histamine; D000255:Adenosine Triphosphate; D002217:Carbachol; D013806:Theophylline; D007213:Indomethacin