The local anti-inflammatory action of dexamethasone in the rat carrageenin oedema model is reversed by an antiserum to lipocortin 1.
1. A local pre-injection of 1 micrograms dexamethasone sodium phosphate strongly inhibited (> 60% inhibition at 3 h; P < 0.001 at all time points) the development of carrageenin-induced paw oedema in the rat induced by a subplantar injection of 0.1 ml, 2% carrageenin. 2. Coinjection of a polyclonal rabbit antiserum raised against human 1-188 recombinant lipocortin 1, which also recognised the rat protein, reversed the inhibitory action of dexamethasone (P < 0.05 at 4 h and 5 h). At the highest volume used (40 microliters) control antisera were without any effect. 3. These data further support the concept that lipocortin 1 is involved in the anti-inflammatory mechanism of action of the glucocorticoids.
Duncan
G S
GS
Department of Biochemical Pharmacology, William Harvey Research Institute, Medical College of St. Bartholomew's Hospital, Charterhouse Square, London.
Peers
S H
SH
Carey
F
F
Forder
R
R
Flower
R J
RJ
D000818:Animals; D017305:Annexin A1 / Q000276:immunology / Q000502:physiology*; D015153:Blotting, Western; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000008:administration & dosage / Q000276:immunology; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D017305:Annexin A1; D007106:Immune Sera; D003907:Dexamethasone