Metabolic pathway characterization from transient response data obtained in situ: parameter estimation in S-system models.
The actual values of internal metabolites and fluxes can be measured by a number of experimental techniques and they provide important information for evaluating the properties of a metabolic pathway in situ. In this paper we propose a strategy to properly exploit this information. The suggested approach permits estimation of a set of parameters on the whole system so that a useful model can be constructed and used to describe its components and systemic properties and to predict its behavior under new conditions. A simulated reference pathway is provided to validate this method and to show its utility in metabolic studies.
Sorribas
A
A
Departament de Ciències Mèdiques Bàsiques, Universitat de Barcelona, Catalunya, Spain.
Samitier
S
S
Canela
E I
EI
Cascante
M
M
D000818:Animals; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D008660:Metabolism / Q000502:physiology*; D008954:Models, Biological