Identification and functional significance of nicotinic cholinergic receptors in the rat pineal gland.
The existence and subunit identification of the nicotinic cholinergic receptors in the rat pineal gland were examined by autoradiography, using [3H]cytisine and [125I]alpha-bungarotoxin as labelled ligands. The experiments performed with radioactive cytisine did not reveal specific binding, while iodinated alpha-bungarotoxin disclosed moderate specific binding density, suggesting that the nicotinic cholinergic receptor in the rat pineal is structurally organized with the alpha 7 or alpha 8 subunits present, the only ones that bind alpha-bungarotoxin with high affinity. In vitro functional experiments using pineal explants demonstrated that the binding site may represent a readily accessible nicotinic cholinergic receptor. Nicotine, though having no effect per se on the synthesis and release of melatonin, significantly diminished, in a dose-dependent manner, the norepinephrine-stimulated melatonin accumulation. This effect could be blocked by coincubation with the cholinergic antagonist d-tubocurarine, suggesting that the nicotinic cholinergic receptor in the rat pineal could be involved in the functional regulation of the gland.
Stankov
B
B
Department of Pharmacology, University of Milan, Italy.
Cimino
M
M
Marini
P
P
Lucini
V
V
Fraschini
F
F
Clementi
F
F
D000470:Alkaloids / Q000378:metabolism; D000818:Animals; D001392:Azocines; D002038:Bungarotoxins / Q000378:metabolism; D007457:Iodine Radioisotopes; D008297:Male; D008550:Melatonin / Q000378:metabolism*; D009538:Nicotine / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology*; D009924:Organ Culture Techniques; D010870:Pineal Gland / Q000378:metabolism*; D011807:Quinolizines; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D012016:Reference Values; D012741:Sexual Maturation; D014403:Tubocurarine / Q000494:pharmacology*; D000093843:Quinolizidine Alkaloids
D000470:Alkaloids; D001392:Azocines; D002038:Bungarotoxins; D007457:Iodine Radioisotopes; D008550:Melatonin; D009538:Nicotine; D009638:Norepinephrine; D011807:Quinolizines; D011978:Receptors, Nicotinic; D014403:Tubocurarine; C004712:cytisine; D000093843:Quinolizidine Alkaloids