Proton magnetic resonance spectroscopy of vitreal changes in experimental streptococcal endophthalmitis.
We used magnetic resonance spectroscopy to examine endophthalmitis in rabbits inoculated with a virulent strain of Streptococcus pneumoniae. On different days after infection, the animals were sacrificed and the vitreous isolated and examined with water-suppressed proton magnetic resonance spectroscopy. A broad resonance corresponding to the methyl envelope of lipoprotein lipids appeared 2 days after infection and persisted until the eyes developed phthisis (around 10 days postinfection). This resonance was absent in the control eye and the bacterial culture; it could be used as the marker of breakdown of blood-vitreous barrier and onset of endophthalmitis-induced changes.
Cheng
H M
HM
Howe Laboratory of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
Hughes
M S
MS
Lashkari
K
K
Sang
D
D
Yoshida
A
A
McMeel
J W
JW
Baker
A S
AS
D000818:Animals; D015415:Biomarkers; D004195:Disease Models, Animal; D009877:Endophthalmitis / Q000378:metabolism* / Q000382:microbiology; D015818:Eye Infections, Bacterial / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011008:Pneumococcal Infections / Q000378:metabolism*; D011817:Rabbits; D014822:Vitreous Body / Q000378:metabolism*
D015415:Biomarkers