title: Inhibition of Rev activity and human immunodeficiency virus type 1 replication by antisense oligodeoxynucleotide phosphorothioate analogs directed against the Rev-responsive element.
abstract: The interaction between the Rev protein of human immunodeficiency virus type 1 and its highly structured and conserved RNA target, the Rev-responsive element, is required for virus replication. We demonstrate that antisense oligodeoxynucleotide phosphorothioate analogs directed against the Rev-responsive element effectively inhibit Rev activity, as well as human immunodeficiency virus type 1 replication, and are candidates for antiviral therapy.
authors.affiliation: Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20892.
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Lisziewicz
authors.forename: D
authors.initials: D
authors.lastname: Sun
authors.forename: G
authors.initials: G
authors.lastname: Zon
authors.forename: S
authors.initials: S
authors.lastname: Daefler
authors.forename: F
authors.initials: F
authors.lastname: Wong-Staal
authors.forename: R C
authors.initials: RC
authors.lastname: Gallo
authors.forename: M E
authors.initials: ME
authors.lastname: Klotman
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D015694:Gene Products, rev / Q000037:antagonists & inhibitors*; D015658:HIV Infections / Q000517:prevention & control*; D015497:HIV-1 / Q000254:growth & development* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012367:RNA, Viral / Q000648:ultrastructure; D012045:Regulatory Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D014779:Virus Replication / Q000187:drug effects; D054321:rev Gene Products, Human Immunodeficiency Virus
chemical_list: D015694:Gene Products, rev; D016376:Oligonucleotides, Antisense; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus