Transforming growth factor-beta (TGF-beta) in human milk.
The amount of TGF-beta contained in human whey was studied by the colony formation of NRK47F cells. It was noted that a factor inducing colony formation did exist in human whey, and its action was neutralized when anti-TGF-beta antibodies were introduced. This suggests that TGF-beta does exist in human whey. In colostrum, the total amount of TGF-beta was 1365.7 +/- 242.9 ng/ml, of which the active form comprised 728.1 +/- 248.7 ng/ml (n = 21). In late milk, the total TGF-beta was 952.5 +/- 212.6 ng/ml, with an active form of 178.7 +/- 157.3 ng/ml. Thus human milk contains a large amount of active TGF-beta. Furthermore, it was revealed by the reverse transcriptase polymerase chain reaction that mRNAs coding TGF-beta 1 and TGF-beta 2 exist in human milk cells. These results suggest that both TGF-beta 1 and TGF-beta 2 exist in human milk.
Saito
S
S
Department of Obstetrics and Gynaecology, Nara Medical University, Japan.
Yoshida
M
M
Ichijo
M
M
Ishizaka
S
S
Tsujii
T
T
D001483:Base Sequence; D003126:Colostrum / Q000737:chemistry; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta