Molecular cloning and sequence of a novel ommochrome-binding protein cDNA from an insect, Manduca sexta.
An ommochrome-binding protein (OBP) from the hemolymph of Manduca sexta has recently been purified and characterized. A cDNA clone was isolated from a fifth instar larval cDNA expression library utilizing antiserum against OBP. Northern blot analysis of total fat body RNA detected a transcript of approximately 1.2 kilobases in fifth instar wandering larvae RNA. The complete nucleotide sequence of the 905-base pair cDNA insert was determined by the dideoxy chain termination method. The OBP cDNA encodes a polypeptide of 274 residues with a predicted molecular weight of 30,580 and with one consensus N-linked glycosylation site. Comparison of the NH2-terminal sequence of the mature protein and the cDNA sequence revealed a typical signal peptide of 18 amino acids. In wandering stage larvae, the OBP transcript appeared to be at least 250-fold less abundant than ribosomal RNA.
Yepiz-Plascencia
G M
GM
Department of Biochemistry, University of Arizona, Tucson 85721.
Ho
C
C
Martel
R R
RR
Law
J H
JH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D017344:Genes, Insect; D006458:Hemolymph / Q000737:chemistry; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D010640:Phenothiazines / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
D002352:Carrier Proteins; D007301:Insect Hormones; D019476:Insect Proteins; C071652:OBP protein, Manduca sexta; D010640:Phenothiazines; D012333:RNA, Messenger; C002772:ommochrome; D004247:DNA