Gametophytic and sporophytic expression of an anther-specific Arabidopsis thaliana gene.
Genomic and cDNA clones of the anther-specific APG gene from Arabidopsis thaliana and Brassica napus, which encodes a novel proline-rich protein, were isolated and characterized. Southern blotting and Northern analysis of male fertile and cytoplasmic male sterile varieties of B. napus showed that the APG gene is present as a single copy in the Arabidopsis genome, and that the B. napus APG gene is a member of a small anther-specific gene family. Analysis of developmentally staged B. napus flower buds indicated that APG transcript is confined to the anther during the period of microspore development. Reporter gene fusions established that the APG promoter directs expression in a number of cell types in anthers of transformed plants. This expression is consistent with the temporal pattern of mRNA accumulation in B. napus buds and follows a complex developmental pattern. Most significantly, the promoter is active in both sporophytic and gametophytic cell types, with activity of the transgene in each cell type being delineated by various cytological markers.
Roberts
M R
MR
Department of Botany, University of Leicester, UK.
Foster
G D
GD
Blundell
R P
RP
Robinson
S W
SW
Kumar
A
A
Draper
J
J
Scott
R
R
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins; D001937:Brassica / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D005718:Gametogenesis / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011058:Pollen / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013170:Spores; D014158:Transcription, Genetic
C083695:APG protein, Arabidopsis; C083696:APG protein, Brassica napus; D029681:Arabidopsis Proteins; D010940:Plant Proteins; C079356:oleosin protein, Brassica napus; D004247:DNA; D005966:Glucuronidase