Identification of two new members of the 1-aminocyclopropane-1-carboxylate synthase-encoding multigene family in mung bean.
The key enzyme regulating ethylene biosynthesis in higher plants is 1-aminocyclopropane-1-carboxylate (ACC) synthase. In mung bean (MB), the existence of three genes encoding this enzyme has previously been reported [Botella et al., Plant Mol. Biol. 18 (1992) 793-797], one of which corresponds to a full-length indole-3-acetic acid-inducible cDNA [Botella et al., Plant Mol. Biol. (1992) 425-436]. In this paper we report the cloning of two new genomic sequences coding for ACC synthase in MB (MAC-4 and MAC-5). MAC-4 is 1340 bp long and encodes 388 amino acids (aa) while MAC-5 is 1393 bp long and encodes for 391 aa. Genomic Southern analysis suggests the existence of only one copy of each gene in the genome.
Botella
J R
JR
Department of Horticulture, Pennsylvania State University, University Park 16802.
Schlagnhaufer
C D
CD
Arteca
J M
JM
Arteca
R N
RN
Phillips
A T
AT
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics*; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D010802:Phylogeny; D010946:Plants, Medicinal
D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase