Analysis of secretion and expression of platelet-derived growth factor in cultured endothelial cells from stroke-prone spontaneously hypertensive rat.
1. We characterized the endothelial cell-derived growth factors of SHRSP and Wistar Kyoto rats (WKY), respectively and found that platelet-derived growth factor (PDGF)-B chain related growth factor constituted a major portion of the mitogenic activity of the conditioned media of endothelial cells from both animals. There were no remarkable qualitative differences between the endothelial cell-derived growth factors of SHRSP and WKY. 2. Northern analysis revealed that the expression of PDGF-B chain was 2-4-fold enhanced in cultured aortic endothelial cells of SHRSP. This enhanced expression of PDGF-B chain, which may be induced under chronic hypertensive conditions, is suggested to contribute to the increase in endothelial cell-derived growth factors reported in this animal.
Iihara
K
K
Department of Neurosurgery, Kyoto University, Faculty of Medicine, Japan.
Sasahara
M
M
Saeki
Y
Y
Hashimoto
N
N
Kikuchi
H
H
Hazama
F
F
D016475:3T3 Cells; D000818:Animals; D001011:Aorta / Q000378:metabolism; D015152:Blotting, Northern; D002455:Cell Division; D002478:Cells, Cultured; D002561:Cerebrovascular Disorders / Q000209:etiology*; D017077:Culture Media, Conditioned; D004198:Disease Susceptibility; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D051379:Mice; D008934:Mitogens; D009500:Neutralization Tests; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D017077:Culture Media, Conditioned; D008934:Mitogens; D010982:Platelet-Derived Growth Factor