Regulation of Cu,Zn- and Mn-superoxide dismutase transcription in Saccharomyces cerevisiae.
The regulation of Cu,Zn- and Mn-superoxide dismutases (SOD) was investigated by Northern blotting and gene fusions of SOD1 and SOD2 promoters with the beta-galactosidase reporter gene. Cu,ZnSOD expression was increased 3-fold under glucose derepressing conditions, and decreased 4- to 6-fold by oxygen or heme deficiency. MnSOD expression was increased 5-fold by glucose derepression, and decreased 8- to 10-fold by anaerobiosis and 4- to 5-fold by heme deficiency. Induction by paraquat was modest, about 50% for SOD1 and 100% for SOD2; it was apparently independent of the respiratory chain function.
Galiazzo
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F
Institut Jacques Monod, Laboratoire de Biochimie des Porphyrines, Université Paris, France.
Labbe-Bois
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R
D002244:Carbon / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006418:Heme / Q000378:metabolism; D006358:Hot Temperature; D010100:Oxygen / Q000494:pharmacology; D010269:Paraquat / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013482:Superoxide Dismutase / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D006418:Heme; D002244:Carbon; D013482:Superoxide Dismutase; D010269:Paraquat; D010100:Oxygen