Thymosin beta-10 expression in melanoma cell lines and melanocytic lesions: a new progression marker for human cutaneous melanoma.
When screening a subtraction library for sequences that were specifically expressed in highly metastatic human melanoma cell lines, a cDNA clone was isolated encoding thymosin beta-10. We found that expression of thymosin beta-10 mRNA was associated with metastatic behavior of various human melanoma cell lines in nude mice. Furthermore, Northern blot analysis showed that also in freshly harvested human melanocytic lesions thymosin beta-10 was differentially expressed. Although expression of thymosin beta-10 was also examined in other non-melanoma model systems and materials, no clear relation could be established with metastatic potential or malignancy. Therefore, we conclude that thymosin beta-10 can be considered as a new progression marker for human cutaneous melanoma.
Weterman
M A
MA
Department of Biochemistry, University of Nijmegen, The Netherlands.
van Muijen
G N
GN
Ruiter
D J
DJ
Bloemers
H P
HP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008545:Melanoma / Q000737:chemistry* / Q000556:secondary; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D051381:Rats; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D013947:Thymosin; C039302:thymosin beta(10)