title: Natural killer cytotoxicity and antibody-dependent cytotoxicity of cells of rat metrial glands.
abstract: Cell suspensions of rat metrial gland tissue, containing healthy granulated metrial gland (GMG) cells, were assessed for their ability to lyse natural killer (NK) cell target Yac-1 myeloma cells in a [51Cr]-release cytotoxicity assay. Cytotoxicity indices were found to be low even after in vivo stimulation of NK cytotoxic activity by polyinosilic-cytidilic acid and the addition of interferon to test cultures. It is concluded that cell suspensions of rat metrial gland, and rat GMG cells in particular, do not exhibit NK cytotoxicity. However, when a heat inactivated rabbit anti-Yac-1 serum was added to assay cultures, cytotoxicity indices above background levels were obtained, indicating that rat metrial glands contain cells capable of antibody-dependent cytotoxicity.
authors.affiliation: Faculty of Medicine, University of Southampton, UK.
authors.forename: I J
authors.initials: IJ
authors.lastname: Stewart
authors.forename: S
authors.initials: S
authors.lastname: Peel
mesh_terms: D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D008791:Metrial Gland / Q000166:cytology / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D007106:Immune Sera