title: Expression of the colony-stimulating factor 1 receptor in B lymphocytes.
abstract: The FMS proto-oncogene encodes for the colony-stimulating factor 1 receptor (CSF-1R), whose expression within the haematopoietic system has previously been thought to be restricted to cells of the mononuclear phagocyte lineage. We have studied the expression of the CSF-1R in peripheral blood mononuclear cells by indirect immunofluorescence and flow cytometry. FMS expression was detected on both monocytes and B lymphocytes from all samples analysed, including 14 haematologically normal individuals and 31 patients (23 in remission following cytotoxic therapy for lymphoma, six with B-cell chronic lymphocytic leukaemia and two with chronic myelomonocytic leukaemia). The level of FMS expression on B lymphocytes was lower than the level of expression detected on monocytes isolated from the same sample. FMS mRNA expression in B lymphocytes has been confirmed by a reverse transcription-polymerase chain reaction (RT-PCR)-based technique and Northern blot analysis. Thus, FMS may play a role in the normal function of B lymphocytes and, because of its potential oncogenic activity, may contribute to the pathogenesis of malignancies of this cell type.
authors.affiliation: Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
authors.forename: A H
authors.initials: AH
authors.lastname: Baker
authors.forename: S A
authors.initials: SA
authors.lastname: Ridge
authors.forename: T
authors.initials: T
authors.lastname: Hoy
authors.forename: P G
authors.initials: PG
authors.lastname: Cachia
authors.forename: D
authors.initials: D
authors.lastname: Culligan
authors.forename: P
authors.initials: P
authors.lastname: Baines
authors.forename: J A
authors.initials: JA
authors.lastname: Whittaker
authors.forename: A
authors.initials: A
authors.lastname: Jacobs
authors.forename: R A
authors.initials: RA
authors.lastname: Padua
mesh_terms: D001402:B-Lymphocytes / Q000737:chemistry*; D001483:Base Sequence; D015870:Gene Expression; D016236:Genes, fms; D006801:Humans; D008223:Lymphoma / Q000378:metabolism; D008969:Molecular Sequence Data; D009000:Monocytes / Q000737:chemistry; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000032:analysis; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis*
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D016186:Receptor, Macrophage Colony-Stimulating Factor