title: A flow cytometric rosetting assay for the analysis of Fc receptors and C3 receptors on HSV-infected cells.
abstract: A sensitive and reproducible flow cytometric assay was developed for the analysis of Fc gamma and C3b(i) receptors on HSV-infected cells. The method is based on a rosette technique using fluorochrome-labeled erythrocytes sensitized with IgG or C3b(i). A comparison of flow cytometric and microscopic quantitation demonstrated that the binding of EIgG, EC3b(i) to HSV-infected cells were correlated. Flow cytometric analysis provides the opportunity to study simultaneously the distribution of E per HSV-infected cell and the total binding of E to the whole population of HSV-infected cells. Receptor activity and HSV glycoprotein cell surface expression were shown to be correlated in a linear fashion. The assay could be applied to other Fc gamma R- and C3b(i)R-bearing cells.
authors.affiliation: Eijkman-Winkler Laboratory of Medical Microbiology, Utrecht University, Netherlands.
authors.forename: K E
authors.initials: KE
authors.lastname: Van Vliet
authors.forename: L A
authors.initials: LA
authors.lastname: De Graaf-Miltenburg
authors.forename: J
authors.initials: J
authors.lastname: Verhoef
authors.forename: J A
authors.initials: JA
authors.lastname: Van Strijp
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D006023:Glycoproteins / Q000032:analysis; D006561:Herpes Simplex / Q000276:immunology*; D006801:Humans; D051379:Mice; D008853:Microscopy; D017463:Receptors, Complement 3b / Q000032:analysis*; D011961:Receptors, Fc / Q000032:analysis*; D012397:Rosette Formation; D012756:Sheep; D014764:Viral Proteins / Q000032:analysis
chemical_list: D006023:Glycoproteins; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; D014764:Viral Proteins