Enhancement of synaptic facilitation during the progression of kindling epilepsy by amygdala stimulations.
1. A quantitative analysis of facilitation during the kindling stimulation to the amygdala was conducted by measuring the area between the excitatory potential and the baseline in the averaged tetanic response recorded at the entorhinal cortex. The changes in facilitation were then compared with the development of electrographic afterdischarges (AD) and behavioral seizures in response to successive kindling stimulations. 2. Kindling train pulses (n = 99 or 100; duration: 0.5 ms; frequency: 10 Hz; intensity: AD threshold) were applied to conscious rats until at least one generalized seizure occurred or until 13 stimuli were delivered. 3. Facilitation of the entorhinal responses by kindling stimulation first occurred in the monosynaptic excitatory component and was then followed by a progressive increase in the polysynaptic component that was manifested as the later negative peaks. A clear progressive enhancement was observed in the facilitation by successive kindling stimulations, which also induced prolongation of the AD duration and progression of the seizure stages, indicating that activity-dependent enhancement of facilitation (EF) occurred during the progression of kindling epilepsy. 4. Quantitative analysis revealed that the EF that occurred with the progression of seizure stages was statistically significant (P < 0.001, Friedman test). The AD duration (r = 0.89) and the long-term potentiation (r = 0.85) of the entorhinal responses by single test amygdala stimuli showed a very good linear relation to the EF.(ABSTRACT TRUNCATED AT 250 WORDS)
Matsuura
S
S
Department of Physiology, Osaka City University Medical School, Japan.
Hirayama
K
K
Murata
R
R
D000679:Amygdala / Q000503:physiopathology*; D000818:Animals; D001931:Brain Mapping; D004292:Dominance, Cerebral / Q000502:physiology; D004558:Electric Stimulation; D004562:Electrocardiography; D004827:Epilepsy / Q000503:physiopathology*; D005071:Evoked Potentials / Q000502:physiology; D007696:Kindling, Neurologic / Q000502:physiology*; D008032:Limbic System / Q000502:physiology; D017774:Long-Term Potentiation / Q000502:physiology; D008297:Male; D009434:Neural Pathways / Q000503:physiopathology; D009474:Neurons / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000502:physiology; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*
D016194:Receptors, N-Methyl-D-Aspartate