title: Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.
abstract: The tsBN7 cell line, one of the mutant lines temperature sensitive for growth which have been isolated from the BHK21 cell line, was found to die by apoptosis following a shift to the nonpermissive temperature. The induced apoptosis was inhibited by a protein synthesis inhibitor, cycloheximide, but not by the bcl-2-encoded protein. By DNA-mediated gene transfer, we cloned a cDNA that complements the tsBN7 mutation. It encodes a novel hydrophobic protein, designated DAD1, which is well conserved (100% identical amino acids between humans and hamsters). By comparing the base sequences of the parental BHK21 and tsBN7 DAD1 cDNAs, we found that the DAD1-encoding gene is mutated in tsBN7 cells. The DAD1 protein disappeared in tsBN7 cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggers apoptosis.
authors.affiliation: Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakashima
authors.forename: T
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: A
authors.initials: A
authors.lastname: Kuraoka
authors.forename: K
authors.initials: K
authors.lastname: Fukushima
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
authors.forename: S
authors.initials: S
authors.lastname: Komiyama
authors.forename: T
authors.initials: T
authors.lastname: Nishimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D017124:Conserved Sequence; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics* / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D029867:Xenopus Proteins; D014982:Xenopus laevis
chemical_list: D051017:Apoptosis Regulatory Proteins; C489988:DAD1 protein, Xenopus; C083331:DAD1 protein, human; D008565:Membrane Proteins; D011506:Proteins; D029867:Xenopus Proteins; D004247:DNA; D003513:Cycloheximide