title: Comparison of K-ras oncogene activation in pancreatic duct carcinomas and cholangiocarcinomas induced in hamsters by N-nitrosobis(2-hydroxypropyl)amine.
abstract: The presence of K-ras point mutations in pancreatic duct carcinomas and cholangiocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP) in Syrian hamsters was investigated by single-strand conformation polymorphism analysis of polymerase chain reaction products from frozen fresh materials in order to clarify the K-ras mutation rates in those two carcinomas induced simultaneously by one carcinogen, BHP. In the examined pancreatic duct carcinomas, 10 out of 16 were positive for a mutation in codon 12 while 3 out of 12 cholangiocarcinomas demonstrated mutation of K-ras gene. G-to-A transition was detected in the second position of codon 12 in both pancreatic carcinomas and cholangiocarcinomas. These results suggest that the role of genetic alteration in carcinogenesis may differ with the target organ, even when initiation is with the same carcinogen.
authors.affiliation: Department of Oncological Pathology, Nara Medical University.
authors.forename: M
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Kondoh
authors.forename: T
authors.initials: T
authors.lastname: Tsujiuchi
authors.forename: K
authors.initials: K
authors.lastname: Hohnoki
authors.forename: K
authors.initials: K
authors.lastname: Horiguchi
authors.forename: O
authors.initials: O
authors.lastname: Noguchi
authors.forename: E
authors.initials: E
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Okita
authors.forename: T
authors.initials: T
authors.lastname: Sekiya
mesh_terms: D000818:Animals; D001483:Base Sequence; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced / Q000235:genetics*; D018281:Cholangiocarcinoma / Q000139:chemically induced / Q000235:genetics*; D006224:Cricetinae; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009602:Nitrosamines; D010183:Pancreatic Ducts; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction
chemical_list: D002273:Carcinogens; D009602:Nitrosamines; C012457:diisopropanolnitrosamine