Molecular characterization of the cDNA coding for translation elongation factor-2 of pathogenic Entamoeba histolytica.
To investigate the humoral immune response of patients with amoebic dysentry against Entamoeba histolytica, immunoglobulin G (IgG)-immunopositive cDNA clones from the pathogenic strain SFL-3 were examined. A large part of the IgG-positive cDNA clones obtained with one serum encoded highly conserved intracellular proteins. A clone was found that was homologous to translation elongation factor-2 (EF-2). Sequence analysis of the EF-2 cDNA showed 63.6% amino acid sequence identity with the human homologue. The deduced protein sequence has a length of 840 amino acid residues with a molecular mass of 93.3 kD. The 3' and 5' untranslated regions of the mRNA are relatively short as shown for other genes of E. histolytica. A genomic clone was used to analyze the region upstream of the translation initiation codon. The codon distribution of EF-2 and other published E. histolytica sequences reflects the high A/T content. The codons for different amino acids are biased to a widely differing extent.
Plaimauer
B
B
Institute of Specific Prophylaxis and Tropical Medicine, University of Vienna, Austria.
Ortner
S
S
Wiedermann
G
G
Scheiner
O
O
Duchêne
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000954:Antigens, Surface / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000737:chemistry*; D004404:Dysentery, Amebic / Q000276:immunology; D004748:Entamoeba histolytica / Q000235:genetics* / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D016053:RNA, Protozoan / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D000913:Antibodies, Protozoan; D000954:Antigens, Surface; D016054:DNA, Protozoan; D007074:Immunoglobulin G; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D015800:Protozoan Proteins; D016053:RNA, Protozoan