Lipid composition of intestinal brush border membrane in alloxan induced acute experimental diabetes.
Several alterations were observed in the rat intestinal brush border membrane (BBM) lipid composition after 7 days old alloxane-induced diabetes as compared to the control animals. There was no change in the total protein contents but a significant increase in the total lipid contents was observed. Glycolipids constituting the major lipid components showed a two-fold increase. No significant difference was observed in the total phospholipid contents. A significant decline in the free cholesterol (CH) level, free fatty acids, triglycerides and sialic acid contents was observed in membranes from diabetic rats. Esterified CH, monoglycerides+diglycerides, phosphatidyl serine+phosphatidyl inositol and phosphatidyl choline levels remained unaffected. A significant increase in sphingomyelin with a parallel decrease in phosphatidyl ethanolamine was observed in BBM preparations from diabetic rats. The observed changes in intestinal BBM might be responsible for altered functions of the diabetic intestines.
Dutt
P
P
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Sarkar
A K
AK
D000496:Alloxan; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D007408:Intestinal Absorption; D007422:Intestines / Q000737:chemistry*; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis*; D008871:Microvilli / Q000737:chemistry; D051381:Rats
D008563:Membrane Lipids; D000496:Alloxan