Smoking-related DNA adducts: 32P-postlabeling analysis of 7-methylguanine in human bronchial and lymphocyte DNA.
7-methylguanine DNA adducts were determined in macroscopically normal bronchial specimens and peripheral blood lymphocytes of 20 patients undergoing pulmonary surgery. A recently developed 32P-postlabeling assay was applied with anion exchange chromatography as an adduct enrichment method. The material consisted of 13 smokers and 7 non-smokers. The mean bronchial 7-methylguanine levels of 11 smokers and 6 non-smokers were 17.3 and 4.7 adducts/10(7) nucleotides. In lymphocyte DNA, the respective mean levels were 11.5 and 2.3 adducts/10(7) nucleotides. The bronchial DNA adduct levels in smokers were statistically higher than those in non-smokers. Among 5 smokers, for whom both bronchial and lymphocyte DNA was available, 7-methylguanine levels correlated in the two tissues (r = 0.77).
Mustonen
R
R
Department of Industrial Hygiene and Toxicology, Institute of Occupational Health, Helsinki, Finland.
Schoket
B
B
Hemminki
K
K
D000328:Adult; D000368:Aged; D001980:Bronchi / Q000737:chemistry* / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D004249:DNA Damage; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry* / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009602:Nitrosamines / Q000633:toxicity; D010761:Phosphorus Radioisotopes; D012907:Smoking / Q000009:adverse effects*
D002273:Carcinogens; D009602:Nitrosamines; D010761:Phosphorus Radioisotopes; D006147:Guanine; C008450:7-methylguanine; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; D004247:DNA; C008655:N'-nitrosonornicotine