title: Germ line maintenance of the pseudogene donor pool for somatic immunoglobulin gene conversion in chickens.
abstract: Somatic immunoglobulin diversity is generated in avian species by sequential gene conversion of variable (V) gene segments of the immunoglobulin heavy- and light-chain loci during B-cell development. The germ line pools of donor sequence information for somatic V-region gene conversion are found in families of V pseudogenes, located 5' of the single functional V gene of each locus. The sequence relationships among the pseudogenes (psi VL) and functional VL1 gene of the chicken light-chain alleles in three inbred strains were compared to determine the extent of diversity within the germ line pseudogene cluster. Numerous differences were observed. For example, compared with the previously reported CB allele and the G4 allele, the S3 allele contains two intact pseudogenes between psi VL16 and psi VL18. These two adjacent psi VL gene segments (psi VL17a and psi VL17b) could have given rise to the psi VL17 segment of the G4 and CB alleles by homologous recombination. The majority of other sequence polymorphisms among the psi VL alleles appear to be the result of meiotic gene conversion. The incidence of untemplated mutations within psi VL segments is significantly lower than the incidence of mutation within the pseudogene flanking regions. Together with the observations that most psi VL segments have open reading frames and lack stop codons, these data support the hypothesis that the psi VL cluster resembles a functional multigene family maintained by evolutionary selection for its functional role in generating somatic antibody diversity. Meiotic gene conversion events within the psi VL cluster serve both to introduce diversity by the exchange of short segments between family members and to prevent the accumulation of random mutations.
authors.affiliation: Department of Internal Medicine, Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109.
authors.forename: W T
authors.initials: WT
authors.lastname: McCormack
authors.forename: E A
authors.initials: EA
authors.lastname: Hurley
authors.forename: C B
authors.initials: CB
authors.lastname: Thompson
mesh_terms: D000483:Alleles; D000818:Animals; D000916:Antibody Diversity / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D002645:Chickens; D004247:DNA; D005785:Gene Conversion; D005854:Germ Cells; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D004247:DNA