Binomial frequency response to non-binomial pulse sequences for efficient water suppression.
This article reports on the use of short-hard pulse and spin-lock pulse combinations giving a binomial-like frequency response for the measurement of NMR spectra in aqueous solutions of quite dilute samples. The pulse sequence proposed provides excellent water suppression and does not introduce any linear or higher order phase errors. Application to the measurement of 2D NOESY data of a 0.25 mM solution of a double-stranded DNA fragment is presented.
Sodano
P
P
Laboratoire de Résonance Magnétique Nucléaire, Institut Pasteur, Paris, France.
Delepierre
M
M
D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012996:Solutions; D014867:Water / Q000737:chemistry
D012996:Solutions; D014867:Water; D004247:DNA