[Antiribosomal antibodies and the neurological manifestations in systemic lupus erythematosus].
BACKGROUND
The possible positive correlation between the presence of antiribosomic antibodies and neurologic and neuropsychiatric manifestations in patients with systemic lupus erythematosus, cited in the literature, was analyzed.


METHODS
Neurological involvement (current or previous) was evaluated in 71 successive patients. The anti-ENA antibodies (extractable nuclear antigens) were studied with special attention to the antiribosomic antibodies by: a) indirect immunofluorescence (IFI) on triple rat substrate, Hep2 and Crithidia luciliae; b) counter immunoelectrophoresis (CIE); c) double immunodiffusion (DI), and d) Western blot (WB) in the Molt-4 cell line. The statistical study was performed by the Fisher exact test.


RESULTS
RibosomAL dyeing was observed in only 2 patients by IFI on triple rat substrate and HEp2. With CIE and DI, 14 patients (20%) were anti-ENA positive. Detectable bands were obtained with WB in 47 patients (66%) with 9 corresponding to antiribosomic antibodies. No statistical differences were found (p > 0.3) in relation with the presence of antirobosomic antibodies in particular and anti-ENA in general, between the groups with and without neurologic involvement.


CONCLUSIONS
No relation was observed between antiribosomic antibodies and neurolupus by determinations of anti-ENA antibodies by Western blot (superior method--p < 0.0001--to direct immunofluorescence, counter immunoelectrophoresis and double immunodiffusion in the comparative study of sensitivity.
Wichmann Schlipf
I
I
Unidad de Colagenosis, Hospital Universitario Virgen del Rocío, Sevilla.
Sánchez Román
J
J
Castillo Palma
M J
MJ
Varela Aguilar
J M
JM
Martino Albornoz
C
C
Tena Soto
N
N
Núñez Roldán
A
A
D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D005260:Female; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000175:diagnosis*; D008297:Male; D009422:Nervous System Diseases / Q000175:diagnosis* / Q000209:etiology; D015800:Protozoan Proteins; D012269:Ribosomal Proteins / Q000276:immunology*
D001323:Autoantibodies; C064601:L12E protein, Trypanosoma cruzi; D015800:Protozoan Proteins; D012269:Ribosomal Proteins