Growth and phenotypic characterization of Legionella species on semisolid media made with washed agar.
Legionella pneumophila (and 28 Legionella species) grew efficiently on charcoal-free, buffered yeast extract medium made with washed agar and without apparent loss of infectivity for U937 cells. Because charcoal-free, buffered yeast extract is transparent, it is a suitable base for indicator media and pigment detection. In standard media, charcoal apparently prevents agar contaminants from inhibiting Legionella growth.
Rogers
J E
JE
Department of Microbiology and Immunology, University of Michigan, Ann Arbor 48109-0620.
Jones
G W
GW
Engleberg
N C
NC
D000362:Agar / Q000737:chemistry; D002606:Charcoal; D015169:Colony Count, Microbial; D006801:Humans; D007875:Legionella / Q000254:growth & development*; D015003:Yeasts
D002606:Charcoal; D000362:Agar