[Thrombospondin modulates cell attachment, spreading and proliferation in cultured rat mesangial cells].
The effect of thrombospondin (TSP) on attachment, spreading, cell toxicity and proliferation of cultured rat mesangial cells (M cell) were investigated. The results are as follows: 1. Attachment and spreading of M cell on the culture plates coated with TSP were inhibited in a dose dependent manner, 2. 50 micrograms/ml TSP in the medium brought about the significant detachment of the adhered M cell. 3. TSP had no demonstrable effects on 51Cr-release from M cell, 4. 3H-thymidine uptake into M cell was inhibited by TSP in a dose dependent manner. These results indicate that TSP modulates attachment, spreading and proliferation of M cell. Therefore, it is suggested that overproduction of TSP in M cell under some pathophysiologic conditions such as diabetic nephropathy may influence the M cell functions in a self-suppression.
Tsukamoto
M
M
Second Department of Internal Medicine, Toho University School of Medicine, Tokyo, Japan.
Tada
H
H
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000209:etiology; D005260:Female; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects*; D008562:Membrane Glycoproteins / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D019699:Thrombospondins
D008562:Membrane Glycoproteins; D019699:Thrombospondins