A mutant CHO-K1 strain with resistance to Pseudomonas exotoxin A is unable to process the precursor fusion glycoprotein of Newcastle disease virus.
RPE.40, a mutant strain of CHO-K1 cells isolated for resistance to Pseudomonas exotoxin A and cross-resistant to alphaviruses, is also highly resistant to virulent strains of Newcastle disease virus. The resistance of RPE.40 cells to Newcastle disease virus results from the failure to cleave the viral envelope precursor glycoprotein Fo to fusion glycoprotein F1 at the consensus sequence (Lys/Arg)-Arg-Gln-(Lys/Arg)-Arg.
Inocencio
N M
NM
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405-0068.
Moehring
J M
JM
Moehring
T J
TJ
D036002:ADP Ribose Transferases; D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins; D016466:CHO Cells / Q000187:drug effects / Q000382:microbiology*; D016384:Consensus Sequence; D006224:Cricetinae; D004351:Drug Resistance; D005098:Exotoxins / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D009522:Newcastle disease virus / Q000378:metabolism* / Q000472:pathogenicity; D011499:Protein Processing, Post-Translational; D014357:Trypsin / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014760:Viral Fusion Proteins / Q000187:drug effects / Q000378:metabolism*; D014774:Virulence; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D014357:Trypsin; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A