title: Differential shedding of the two subunits of the interleukin-6 receptor.
abstract: cDNAs coding for the two receptor subunits of the interleukin-6 receptor have been stably expressed in Madine Darby canine kidney (MDCK) cells. The fate of the IL-6 binding protein (IL-6R) and of the signal transducing protein gp130 was studied independently. Both proteins were proteolytically cleaved from cells metabolically labeled with [35S]methionine/cysteine leading to the release of soluble receptor proteins of 55 kDa and 100 kDa, respectively. In contrast to the shedding of the IL-6R gp130 was inefficiently released from the cells and the process was not significantly stimulated by the phorbolester PMA. In addition we show that the soluble forms of the IL-6R and gp130 released by transfected cells can form a ternary complex with interleukin-6 indicating that such complexes also may occur in vivo.
authors.affiliation: Institut für Biochemie, RWTH Aachen, Klinikum, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Müllberg
authors.forename: E
authors.initials: E
authors.lastname: Dittrich
authors.forename: L
authors.initials: L
authors.lastname: Graeve
authors.forename: C
authors.initials: C
authors.lastname: Gerhartz
authors.forename: K
authors.initials: K
authors.lastname: Yasukawa
authors.forename: T
authors.initials: T
authors.lastname: Taga
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
authors.forename: P C
authors.initials: PC
authors.lastname: Heinrich
authors.forename: S
authors.initials: S
authors.lastname: Rose-John
mesh_terms: D000818:Animals; D015703:Antigens, CD; D002478:Cells, Cultured; D050822:Cytokine Receptor gp130; D004285:Dogs; D008562:Membrane Glycoproteins / Q000378:metabolism; D011233:Precipitin Tests; D018123:Receptors, Interleukin / Q000187:drug effects / Q000378:metabolism*; D019947:Receptors, Interleukin-6; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D008562:Membrane Glycoproteins; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130; D013755:Tetradecanoylphorbol Acetate