CO2 production in cell-free extracts of fission yeast detects cell cycle changes.
CO2 production was followed by manometry in starved cell-free extracts of fission yeast stimulated by unstarved cell-free extracts from a synchronous culture. The degree of stimulus, measured by the lag time in CO2 production, varied markedly during the cell cycle, with a maximum for cells at about mitosis and a minimum for septated cells. Similar differences in lag time were found with unstarved extracts of cdc13.117 grown at 37 degrees C and 35 degrees C.
Novak
B
B
Institute of Cell, Animal and Population Biology, University of Edinburgh, Ashworth Laboratories, Scotland.
Sveiczer
A
A
Mitchison
J M
JM
D015415:Biomarkers; D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D002474:Cell-Free System; D019926:Cyclin B; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D006019:Glycolysis; D008365:Manometry; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D015415:Biomarkers; D019926:Cyclin B; D016213:Cyclins; D005656:Fungal Proteins; D002245:Carbon Dioxide; D016203:CDC2 Protein Kinase; D005947:Glucose