title: A DNA polymerase alpha-associated 56 kDa protein kinase.
abstract: Initiation of chromosomal DNA replication must be carefully regulated during the cell cycle. We report that Drosophila embryo DNA polymerase alpha complex, isolated by immunological techniques, contains a protein kinase activity. The kinase will phosphorylate histone H1, but prefers peptides contained in the DNA polymerase alpha-kinase complex. Renaturation experiments determined that the kinase activity resides in a 56-kDa peptide.
authors.affiliation: Department of Radiation Oncology, University of Arizona Health Sciences Center, University of Arizona, Tucson 85724.
authors.forename: V M
authors.initials: VM
authors.lastname: Peck
authors.forename: E W
authors.initials: EW
authors.lastname: Gerner
authors.forename: A E
authors.initials: AE
authors.lastname: Cress
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D002846:Chromatography, Affinity; D004257:DNA Polymerase II / Q000302:isolation & purification / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism; D008970:Molecular Weight; D010766:Phosphorylation; D011489:Protein Denaturation; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D000911:Antibodies, Monoclonal; D006657:Histones; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D004257:DNA Polymerase II