Actin and actin-binding proteins in plasma membranes derived from Walker 256 ascites or solid tumour cells.
Plasma membranes from Walker 256 carcinoma cells grown ascitically or as a solid tumour were examined with respect to actin content, [3H]cytochalasin B-binding and the binding of 125I-labelled G-actin to membrane proteins separated by SDS-PAGE. Differences were observed both in cytochalasin B-binding to membrane actin and affinity of 125I-labelled G-actin for specific membrane proteins.
Goodlad
G A
GA
School of Biological and Medical Sciences, Division of Cell and Molecular Biology, University of St. Andrews, UK.
Clark
C M
CM
D000199:Actins / Q000032:analysis*; D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry*; D001665:Binding Sites; D002279:Carcinoma 256, Walker / Q000737:chemistry*; D002352:Carrier Proteins; D002460:Cell Line / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D003571:Cytochalasin B / Q000378:metabolism; D005260:Female; D008840:Microfilament Proteins / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D014336:Troponin
D000199:Actins; D002352:Carrier Proteins; C034037:F-actin-binding proteins; D008840:Microfilament Proteins; D014336:Troponin; D003571:Cytochalasin B