Characterization of murine lymphokine-activated killer cell cultures separated according to cell size.
Incubation of murine splenocytes in high concentrations of the lymphokine interleukin 2 leads to the development of cytotoxic effector cells termed lymphokine-activated killer cells. Two populations of blast cells develop within these cultures, one of T origin and one of NK origin. The T blasts are CD8+, FcR gamma III- and exhibit slightly increased forward angle light scatter (FSC) and side angle light scatter (SSC). They have little or no oncolytic activity against the tumor target YAC-1. The NK blasts are CD8-, FcR gamma III+ and exhibit markedly increased FSC and SSC. They contain virtually all the lytic activity against the tumor target YAC-1. Cultures were split into multiple fractions on the basis of sedimentation velocity, which separates cells primarily on the basis of cell size. The sedimentation separation resolved both T and NK cells into resting (G0) small lymphocytes and cycling blast cells. NK blasts sedimented much more rapidly than all CD8+ cells such that most of the oncolytic activity could be separated relatively free of CD8+ cells.
Chadwick
B S
BS
Ontario Cancer Institute, University of Toronto, Canada.
Brady
G
G
Miller
R G
RG
D000818:Animals; D016827:CD8 Antigens / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D048429:Cell Size; D007376:Interleukin-2; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017452:Receptors, IgG / Q000276:immunology; D016192:Resting Phase, Cell Cycle; D013601:T-Lymphocytes / Q000276:immunology
D016827:CD8 Antigens; D007376:Interleukin-2; D017452:Receptors, IgG