title: Influence of fermentation conditions on specific activity of the enzymes alcohol and aldehyde dehydrogenase from yeasts.
abstract: The effects of anaerobic, semi-aerobic and short aeration fermentation conditions and the addition of ergosterol and oleic acid to musts on the specific activity of alcohol and aldehyde dehydrogenase (ADH and ALDH) from two yeast species, Saccharomyces cerevisiae and Torulaspora delbrueckii, were studied. ADH I biosynthesis only occurred during the first few hours of fermentation. ADH II from S. cerevisiae and ALDH-NADP+ from the two yeast species behaved as constitutive enzymes under all fermentation conditions. ADH II from T. delbrueckii was only synthesized in small amounts, and its activity was always lower than in S. cerevisiae, where it was responsible for the termination of alcoholic fermentation during the steady growth phase.
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Córdoba, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Mauricio
authors.forename: J M
authors.initials: JM
authors.lastname: Ortega
mesh_terms: D000332:Aerobiosis; D000426:Alcohol Dehydrogenase / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D000693:Anaerobiosis; D004875:Ergosterol / Q000494:pharmacology; D005285:Fermentation; D005638:Fruit; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D013045:Species Specificity; D015003:Yeasts / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
chemical_list: D009829:Oleic Acids; D019301:Oleic Acid; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D004875:Ergosterol