Expression of annexins on the surfaces of non-metastatic and metastatic human and rodent tumor cells.
Annexins are a large group of calcium-dependent cytoskeletal- and membrane-associated proteins whose properties include cytoskeleton and phospholipid binding and mitotic signal transduction. Although annexin-like molecules have been reported on the external plasma membranes of certain cells, in general they are considered to be cytoplasmic proteins. We report here the heterogenous expression of certain annexins (I-VI) on the external cell surfaces of non-metastatic and metastatic murine (RAW117 large-cell lymphoma), rat (13762NF mammary adenocarcinoma) and some human (KM12 and HT29 colorectal carcinoma) cell lines but not on some other cell lines such as human (A375 and MeWo) and mouse (B16) melanoma. The implication of annexin cell surface expression in the metastatic process is discussed with respect to tumor cell adhesion.
Yeatman
T J
TJ
Department of General Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Updyke
T V
TV
Kaetzel
M A
MA
Dedman
J R
JR
Nicolson
G L
GL
D000818:Animals; D017302:Annexins / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D009363:Neoplasm Proteins / Q000032:analysis*; D051381:Rats; D014407:Tumor Cells, Cultured
D017302:Annexins; D015815:Cell Adhesion Molecules; D009363:Neoplasm Proteins