Fascin, an echinoid actin-bundling protein, is a homolog of the Drosophila singed gene product.
A cDNA for fascin, an actin-bundling protein in echinoderms, has been cloned, sequenced, and expressed. The predicted mass of the protein is approximately 55 kDa, similar to that observed for fascin purified from sea urchin eggs. Bacterially expressed fascin reacts with antibodies prepared against sea urchin egg fascin. Fascin has a strong sequence similarity to the singed gene (sn) product in Drosophila and has similarities with a 55-kDa human actin-bundling protein. No extensive similarities were found with other known actin-binding/bundling proteins, indicating that this is a separate gene family.
Bryan
J
J
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030-3498.
Edwards
R
R
Matsudaira
P
P
Otto
J
J
Wulfkuhle
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D004331:Drosophila melanogaster / Q000235:genetics*; D004926:Escherichia coli; D005260:Female; D007301:Insect Hormones / Q000737:chemistry / Q000235:genetics*; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010063:Ovum / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012617:Sea Urchins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D017931:DNA Primers; D007301:Insect Hormones; D008840:Microfilament Proteins; C020324:fascin