title: Infection of human synovial cells by human T cell lymphotropic virus type I. Proliferation and granulocyte/macrophage colony-stimulating factor production by synovial cells.
abstract: The present study was performed to clarify the relationship between human T cell lymphotropic virus type I (HTLV-I) infection and chronic inflammatory arthropathy. To determine the ability of HTLV-I to infect synovial cells and the effect on synovial cell proliferation, synovial cells were cocultured with the HTLV-I-producing T cell lines (MT-2 or HCT-1). After coculture with HTLV-I-infected T cells, the synovial cells expressed HTLV-I-specific core antigens, and HTLV-I proviral DNA was detected from the synovial cells by polymerase chain reaction. These cocultured synovial cells with HTLV-I-infected T cells proliferated more actively than the synovial cells cocultured with uninfected T cells. This stimulatory effect of HTLV-I-infected T cells on synovial cell proliferation seems necessary to contact each other. After being cocultured with MT-2 cells, synovial cells proliferated more actively than control cells even after several passages. Furthermore, HTLV-I-infected synovial cells produced significant amounts of granulocyte/macrophage colony-stimulating factor. These results suggest that HTLV-I can infect synovial cells, resulting their active proliferation and may be involved in the pathogenesis of proliferative synovitis similar to that found in rheumatoid arthritis.
authors.affiliation: First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakai
authors.forename: K
authors.initials: K
authors.lastname: Eguchi
authors.forename: K
authors.initials: K
authors.lastname: Terada
authors.forename: M
authors.initials: M
authors.lastname: Nakashima
authors.forename: I
authors.initials: I
authors.lastname: Yamashita
authors.forename: H
authors.initials: H
authors.lastname: Ida
authors.forename: Y
authors.initials: Y
authors.lastname: Kawabe
authors.forename: T
authors.initials: T
authors.lastname: Aoyagi
authors.forename: H
authors.initials: H
authors.lastname: Takino
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag / Q000032:analysis / Q000096:biosynthesis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D016685:Mitomycin / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000379:methods; D011533:Proviruses / Q000502:physiology; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D013601:T-Lymphocytes; D014779:Virus Replication
chemical_list: D017077:Culture Media, Conditioned; D004279:DNA, Viral; D015683:Gene Products, gag; D016685:Mitomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor