Glycyrrhizin as a promoter of the late signal transduction for interleukin-2 production by splenic lymphocytes.
Cellular and molecular mechanisms of the immunomodulatory action of glycyrrhizin (GL) were studied. We demonstrated that GL displays a unique action to prolong the duration of the T-cell receptor-mediated in vitro splenic T-lymphocyte growth response to anti-CD3 monoclonal antibody (mAb) or concanavalin A (Con A) through enhancement of interleukin-2 (IL-2) secretion and IL-2 receptor (IL-2R) expression. The augmentation by GL of IL-2 production was also found in spleen cells stimulated with A23187 plus phorbol 12-myristate 13-acetate (PMA), suggesting that GL primarily affects some post-receptor stage of the signal transduction. We also found that the time of GL action for promoting IL-2 production and growth response was 2 hr or more after receptor activation. Correspondingly, GL did not augment the anti-CD3 mAb or Con A-mediated protein tyrosine phosphorylation and c-fos transcription. We concluded from these results that GL acts as a promoter of the late signal transduction of T lymphocytes for IL-2 production.
Zhang
Y H
YH
Department of Immunology, Nagoya University School of Medicine, Japan.
Isobe
K
K
Nagase
F
F
Lwin
T
T
Kato
M
M
Hamaguchi
M
M
Yokochi
T
T
Nakashima
I
I
D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D002460:Cell Line; D003208:Concanavalin A / Q000276:immunology; D015870:Gene Expression; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000276:immunology; D019695:Glycyrrhizic Acid; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D010766:Phosphorylation; D015398:Signal Transduction / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D014443:Tyrosine / Q000378:metabolism
D017252:CD3 Complex; D007376:Interleukin-2; D003208:Concanavalin A; D014443:Tyrosine; D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid