title: 2-Oxo-histidine as a novel biological marker for oxidatively modified proteins.
abstract: We report a promising finding that oxidative modification of proteins by free radicals could be monitored by the formation of oxidized histidine that is detectable by reverse-phase HPLC with electrochemical detection (HPLC-ECD). When the N-protected histidine derivative (N-benzoylhistidine) was exposed to a free radical-generating system (copper/ascorbate), a number of products were detected by HPLC-ECD and the main product among them was found to be identical to N-benzoyl-2-oxo-histidine. The acid hydrolysis of N-benzoyl-2-oxo-histidine provided a single product (2-oxo-histidine) that was detected sensitively by HPLC-ECD. Thus 2-oxo-histidine was indeed generated as the main product in the oxidatively modified proteins by free radicals. Taken together, 2-oxo-histidine may be a useful biological marker for assessing protein modifications under oxidative stress.
authors.affiliation: Laboratory of Food and Biodynamics, Nagoya University School of Agriculture, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: S
authors.initials: S
authors.lastname: Kawakishi
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006639:Histidine / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D011506:Proteins; D012710:Serum Albumin, Bovine
chemical_list: D015415:Biomarkers; D007202:Indicators and Reagents; D011506:Proteins; C083725:2-oxohistidine; D012710:Serum Albumin, Bovine; D006639:Histidine