title: Characterization of bovine immunodeficiency virus rev cDNAs and identification and subcellular localization of the Rev protein.
abstract: One of the six putative accessory genes of bovine immunodeficiency virus (BIV) is similar to those identified as rev in the human immunodeficiency virus and visna virus genomes. To further analyze the BIV rev gene locus, protein, and function, rev cDNAs were cloned and characterized. BIV rev mRNA is derived from the full-length transcript by multiple splicing events and consists of three exons, including the untranslated leader sequence and two coding exons. BIV rev cDNA was expressed in bacteria and in a mammalian in vitro translation expression system. A 23-kDa Rev protein (p23rev) was immunologically detected in lysates from both systems by using an antiserum made to a synthetic Rev peptide. Recombinant p23rev made in bacteria was purified and used to make a polyvalent antiserum. Antisera to Rev peptide and recombinant p23rev immunoprecipitated p23rev from BIV-infected mammalian cells but not from virions. A mammalian expression vector using the BIV rev cDNA was constructed; p23rev was immunoprecipitated with anti-Rev serum from 32P-labeled lysates of monkey cells transfected with this plasmid, demonstrating that BIV Rev is phosphorylated. Immunofluorescence and immunoelectron microscopy with anti-BIV Rev antisera localized Rev in the nucleus and, particularly, in the nucleoli of BIV-infected cells. In functional studies, the expression of BIV Rev was shown to positively regulate the appearance both of Gag protein, which is translated from the unspliced primary viral transcript, and of singly spliced env mRNA but not that of the multiply spliced tat mRNA. These results demonstrate that BIV Rev activity correlates with the known function of lentivirus Rev proteins.
authors.affiliation: Laboratory of Cell and Molecular Structure, Program Resources, Inc./DynCorp, Frederick, Maryland.
authors.forename: M S
authors.initials: MS
authors.lastname: Oberste
authors.forename: J C
authors.initials: JC
authors.lastname: Williamson
authors.forename: J D
authors.initials: JD
authors.lastname: Greenwood
authors.forename: K
authors.initials: K
authors.lastname: Nagashima
authors.forename: T D
authors.initials: TD
authors.lastname: Copeland
authors.forename: M A
authors.initials: MA
authors.lastname: Gonda
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D015694:Gene Products, rev / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication; D054321:rev Gene Products, Human Immunodeficiency Virus
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D015694:Gene Products, rev; D009687:Nuclear Proteins; D010455:Peptides; D010750:Phosphoproteins; D015678:Viral Structural Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus