Use of polyethylene glycol-bound superoxide dismutase, polyethylene glycol-bound catalase, and nimodipine to prevent hypoxic ischemic injury to the brain of newborn pigs.
OBJECTIVE
To determine if polyethylene glycol-bound superoxide dismutase and catalase and nimodipine, alone or in combination, will ameliorate hypoxic ischemic injury to the brain.


METHODS
A total of 78 newborn (0 to 3 days) pigs were used.


METHODS
Prospective, blinded, randomized, controlled trial.


METHODS
The piglets were subjected to hypoxic ischemic brain injury. Carotid arteries were ligated at time 0 and BP was reduced one third by hemorrhage. At 15 mins, FIO2 was reduced to 0.6. At 30 mins, carotids were released, blood was reinfused, and FIO2 was increased to 1.0. Pigs were randomly assigned at time 35 mins to receive either: 10,000 U/kg of polyethylene glycol-bound superoxide dismutase and catalase (group 1); 0.5 mg/kg of nimodipine (group 2); both 10,000 U/kg of superoxide dismutase and catalase and 0.5 mg/kg of nimodipine (group 3); or no drugs (controls).


METHODS
The time after reoxygenation for return of electroencephalogram, respiration, blink and pain were recorded in minutes as well as a neurologic examination at 1, 2, and 3 days and pathologic examination of the brain at 3 days, both by blinded observers.


RESULTS
There were no significant differences in the four groups.


CONCLUSIONS
Polyethylene glycol-bound superoxide dismutase and catalase, and nimodipine, either alone or in combination, do not ameliorate hypoxic ischemic injury to the brain in the newborn pig when given 5 mins after reoxygenation.
LeBlanc
M H
MH
Department of Pediatrics, University of Mississippi Medical Center, Jackson 39216-4505.
Vig
V
V
Randhawa
T
T
Smith
E E
EE
Parker
C C
CC
Brown
E G
EG
D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose; D001794:Blood Pressure; D001831:Body Temperature; D002545:Brain Ischemia / Q000473:pathology / Q000517:prevention & control*; D002374:Catalase / Q000008:administration & dosage / Q000627:therapeutic use*; D000860:Hypoxia / Q000517:prevention & control*; D007773:Lactates / Q000097:blood; D009553:Nimodipine / Q000008:administration & dosage / Q000627:therapeutic use*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000627:therapeutic use*; D011446:Prospective Studies; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000627:therapeutic use*; D013552:Swine
D001786:Blood Glucose; D007773:Lactates; D011092:Polyethylene Glycols; D009553:Nimodipine; D002374:Catalase; D013482:Superoxide Dismutase; C063471:polyethylene glycol-superoxide dismutase