Isolation and characterization of an ornithine aminotransferase-related sequence (OATL3) mapping to 10q26.
We used a near full-length human ornithine delta-amino-transferase cDNA, huOAT6, as a probe under low stringency hybridization conditions to identify a new autosomal ornithine delta-aminotransferase-related sequence (OATL3). Cloning and characterization of this sequence reveal it to be a partial nonprocessed pseudogene corresponding to exon 3 and flanking intronic sequences of the ornithine delta-aminotransferase structural gene. Using somatic cell hybrids and fluorescence in situ hybridization, we mapped OATL3 to 10q26, adjacent to the ornithine delta-aminotransferase structural gene locus.
Geraghty
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Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Kearns
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Pearson
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Valle
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D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D005091:Exons; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007438:Introns; D007621:Karyotyping; D008677:Metaphase; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics*; D015183:Restriction Mapping
D009838:Oligodeoxyribonucleotides; D004247:DNA; D009953:Ornithine-Oxo-Acid Transaminase