Tissue-specific changes in lipid composition and lipoprotein lipase activity during the development of the chick embryo.
Lipoprotein lipase was present at a high specific activity in adipose tissue and heart of the chick embryo at the 14th day of development. The enzyme was also present in skeletal muscle but was absent from brain and liver. Major increases in the activity of lipoprotein lipase in adipose tissue and heart occurred from day 12 of development, concomitant with the beginning of the period of lipid uptake from the yolk. These results suggest that lipoprotein lipase may be involved in the utilisation of yolk-derived lipid by the tissues of the embryo. Relatively high levels of docosahexaenoic acid (22:6(n--3)) were present in the triacylglycerol isolated from plasma, adipose tissue, heart and liver. The relative proportions of this fatty acid in the triacylglycerol of the different tissues may be explicable in terms of the substrate specificity of lipoprotein lipase.
Speake
B K
BK
Department of Biochemical Sciences, Scottish Agricultural College, Auchincruive, Ayr, UK.
Noble
R C
RC
McCartney
R J
RJ
D000273:Adipose Tissue / Q000196:embryology; D000818:Animals; D002642:Chick Embryo / Q000201:enzymology / Q000254:growth & development*; D004281:Docosahexaenoic Acids / Q000032:analysis; D004527:Egg Proteins / Q000378:metabolism; D006321:Heart / Q000196:embryology; D008055:Lipids / Q000032:analysis*; D008071:Lipoprotein Lipase / Q000032:analysis*; D008954:Models, Biological; D002785:Sterol O-Acyltransferase / Q000032:analysis; D013379:Substrate Specificity; D014280:Triglycerides / Q000737:chemistry / Q000302:isolation & purification
D004527:Egg Proteins; D008055:Lipids; D014280:Triglycerides; D004281:Docosahexaenoic Acids; D002785:Sterol O-Acyltransferase; D008071:Lipoprotein Lipase