Divergent and co-localization of the two small proteoglycans decorin and proteoglycan-100 in human skeletal tissues and tumors.
The core protein of a recently described small proteoglycan, proteoglycan-100, was localized in fetal human tissues by indirect immunocytochemistry and compared with the localization of known members of the small proteoglycan family. Co-localization of decorin and proteoglycan-100 was seen in bone tissue but decorin and proteoglycan-100 exhibited a substantially divergent distribution in fetal skin, cartilage and in the mineralization zone of the growth plate. Proteoglycan-100 was also found in striated muscle, nerve fibers, and synovial tissue. Immunostaining of a chondroblastic osteosarcoma demonstrated chondroid cells selectively expressing either proteoglycan-100 or decorin. Co-expression of both small proteoglycans was observed in sections from a chordoma. In fetal bone and in the two tumors, colocalization of proteoglycan-100 and of biglycan was also found. These results provide evidence of the wide and characteristic distribution of proteoglycan-100.
Bosse
A
A
Gerhard Domagk Institute of Pathology, University of Münster, Germany.
Schwarz
K
K
Vollmer
E
E
Kresse
H
H
D001859:Bone Neoplasms / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism; D002817:Chordoma / Q000378:metabolism; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005333:Fetus / Q000033:anatomy & histology; D006801:Humans; D007150:Immunohistochemistry; D009132:Muscles / Q000378:metabolism; D009412:Nerve Fibers / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism; D011509:Proteoglycans / Q000032:analysis*; D012867:Skin / Q000378:metabolism
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans