Cloning and characterization of an alpha-tubulin-encoding gene from rat-derived Pneumocystis carinii.
The gene and cDNA (TUB1) encoding an alpha-tubulin (alpha-Tub) from Pneumocystis carinii carried in rats were isolated and sequenced. The gene produced a 1.4-kb transcript that contained an open reading frame of 1347 nucleotides coding for a protein of 50,326 Da. The nt sequence of TUB1 of P. carinii was 65-71% identical to other TUB1 genes, including those of yeast and human. The deduced amino acid sequence of P. carinii alpha-Tub showed 75-84% identity to other alpha-Tubs. Eight small introns resided within TUB1. P. carinii TUB1 was mapped to a 425-kb chromosome. The cloned gene appears to be the only alpha-Tub-encoding gene in the P. carinii genome.
Zhang
J
J
Department of Molecular Genetics, Biochemistry and Microbiology, College of Medicine University of Cincinnati, OH 45267-0524.
Stringer
J R
JR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D014404:Tubulin