title: Sequence-tagged NotI sites of human chromosome 21: sequence analysis and mapping.
abstract: We isolated and analyzed 19 NotI site-containing clones specific for human chromosome 21. The overall process consisted of selective isolation of NotI site-containing clones from flow-sorted chromosome 21 libraries, selection of independent clones by their restriction patterns and nucleotide sequences, and assignment of the clones to chromosome 21. Sequence analysis showed that the regions around the NotI sites had features typical of CpG islands, such as extremely high GC content, high-frequency appearance of CpG dinucleotide sequences, and lack of methylation of these CpGs. PCR conditions for these extremely G + C-rich templates were optimized to establish these NotI sites as sequence-tagged sites.
authors.affiliation: Human Genome Center, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hattori
authors.forename: A
authors.initials: A
authors.lastname: Toyoda
authors.forename: H
authors.initials: H
authors.lastname: Ichikawa
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Ohgusu
authors.forename: N
authors.initials: N
authors.lastname: Oishi
authors.forename: T
authors.initials: T
authors.lastname: Kano
authors.forename: S
authors.initials: S
authors.lastname: Kuhara
authors.forename: M
authors.initials: M
authors.lastname: Ohki
authors.forename: Y
authors.initials: Y
authors.lastname: Sakaki
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D002891:Chromosomes, Human, Pair 21; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016324:Sequence Tagged Sites
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases