title: [Detection of minimal residual clone after sex-mismatched bone marrow transplantation by fluorescent in situ hybridization].
abstract: In our previous paper we reported that fluorescent in situ hybridization (FISH) using DYZ1 and DXZ1 was a highly reproducible technique and cellular chimerism consisting of cells of male and female origins could be detected in the order of 0.1%. In the present study FISH was applied to detect minimal residual clones (MRC) in 10 patients with a variety of leukemias who received sex-mismatched bone marrow transplantation (BMT). In 9 patients who had no signs of recurrence, serial FISHs performed on each patient revealed the presence of 0.1 to 3.2% recipient residual clone. On the other hand, in the remaining one patient 10.6% and 16.9% of MRC were found by FISH performed 6 and 10 months after BMT. Immediately thereafter, this patient was diagnosed as in relapse by bone marrow examination.
authors.affiliation: Department of Hygiene, Kyoto Prefectural University of Medicine.
authors.forename: T
authors.initials: T
authors.lastname: Ariyama
authors.forename: J
authors.initials: J
authors.lastname: Inazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Okuma
authors.forename: K
authors.initials: K
authors.lastname: Nagai
authors.forename: A
authors.initials: A
authors.lastname: Horiuchi
authors.forename: T
authors.initials: T
authors.lastname: Abe
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology / Q000628:therapy; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000276:immunology; D002648:Child; D002999:Clone Cells; D005260:Female; D006085:Graft Survival; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000276:immunology; D006648:Histocompatibility; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000276:immunology / Q000628:therapy; D008297:Male; D011379:Prognosis; D012730:Sex Chromosomes