Genes required for extracellular secretion of enterotoxin are clustered in Vibrio cholerae.
Pleiotropic transposon insertion mutants of Vibrio cholerae that are unable to secrete enterotoxin, HA/protease and chitinase through the outer membrane have been isolated. The gene, epsM, responsible for complementation of two of the Tn5 insertion mutations was sequenced. It encodes a putative cytoplasmic membrane protein of 18.5 kDa that exhibits similarity to proteins required for extracellular secretion of pullulanase, pectate lyase or elastase in other Gram-bacteria. It is present on a 15-kb DNA fragment from the V. cholerae genome, containing the epsE gene that was previously shown to be required for secretion of cholera toxin [Sandkvist et al., Gene 123 (1993) 81-86]. Partial reading frames flanking epsM also demonstrated similarity to genes required for extracellular secretion of pullulanase in Klebsiella oxytoca.
Overbye
L J
LJ
Department of Microbiology, Michigan State University, East Lansing 48824.
Sandkvist
M
M
Bagdasarian
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements; D015252:Deoxyribonucleases, Type II Site-Specific; D004768:Enterotoxins / Q000378:metabolism*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D010455:Peptides / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014734:Vibrio cholerae / Q000235:genetics*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004768:Enterotoxins; C079398:EpsE protein, Vibrio cholerae; C083759:EpsM protein, Vibrio cholerae; D008565:Membrane Proteins; D010455:Peptides; C037420:stN protein, Vibrio cholerae; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases