title: Sodium-lithium countertransport activity is not affected by short-term insulin exposure in vivo or in a physiologic medium in vitro.
abstract: This study examined the acute effects of physiologic concentrations of insulin in vitro and in vivo on sodium-lithium countertransport (SLC) kinetics in nondiabetic subjects. SLC was measured at eight different external sodium concentrations including the standard 150 mmol/L, allowing calculation of both maximal velocity (Vmax) and external sodium affinity (Km). Incubation with insulin (50 mU/L) in 110 MgCl2 but not in 150 mmol/L NaCl decreased standard SLC activity. The decrease was accounted for by a reduction in Vmax, whereas Km remained constant. There was no difference in standard SLC activity, Vmax, or Km when endogenous insulin concentrations were altered either by fasting or by a carbohydrate load. Similarly, standard SLC activity, Vmax, or Km were not significantly different before or at the completion of a euglycemic hyperinsulinemic clamp. These findings provide no support for an action of physiologic concentrations of insulin either in vitro or in vivo on the kinetics of the countertransporter measured in vitro in isotonic sodium-containing media.
authors.affiliation: Department of Nephrology, University of Newcastle-upon-Tyne, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: Rutherford
authors.forename: T H
authors.initials: TH
authors.lastname: Thomas
authors.forename: T
authors.initials: T
authors.lastname: Hardman
authors.forename: A F
authors.initials: AF
authors.lastname: Lant
authors.forename: R
authors.initials: R
authors.lastname: Wilkinson
mesh_terms: D000328:Adult; D017920:Antiporters / Q000378:metabolism*; D003470:Culture Media; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009994:Osmolar Concentration; D013997:Time Factors
chemical_list: D017920:Antiporters; D003470:Culture Media; D007328:Insulin; C045885:sodium-lithium countertransporter; D005947:Glucose