title: Characterization of a new sporulation factor in Bacillus subtilis.
abstract: We report the existence and partial purification of sporulation factor, which stimulates sporulation of Bacillus subtilis at low cell density. Proline or arginine is required for stimulation under the conditions of our assay. Sporulation factor is a small heat-stable substance produced by the cells during exponential growth phase. It is required in small amounts and is resistant to various proteolytic agents. Several spo mutants were tested for the ability to produce functional sporulation factor. All of these mutants produce factor and do not sporulate in the presence of factor from wild-type cells. Sporulation factor is not involved in the induction of alpha-amylase synthesis at the initiation of sporulation.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison 53706.
authors.forename: C
authors.initials: C
authors.lastname: Waldburger
authors.forename: D
authors.initials: D
authors.lastname: Gonzalez
authors.forename: G H
authors.initials: GH
authors.lastname: Chambliss
mesh_terms: D001120:Arginine / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006639:Histidine / Q000494:pharmacology; D007288:Inosine / Q000494:pharmacology; D007700:Kinetics; D011392:Proline / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology; D000516:alpha-Amylases / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D006639:Histidine; D007288:Inosine; D001120:Arginine; D011392:Proline; D000516:alpha-Amylases