title: Effect of age on the ability of rat liver tissues to transform chemical promutagens to mutagens.
abstract: Age-related changes in drug metabolism of the liver from male Lou rats were determined by measuring changes in the production of mutagens. Activation of aflatoxin B1 (AFB1), 2-aminoanthracene (2AA) and benzo[a]pyrene (B[a]P) to mutagenic derivatives was assayed using the Ames salmonella test system. The promutagens were incubated with tissue fractions isolated from rats ranging in age from 3 weeks to 18 months. Hepatic activation of AFB1 and 2AA changes with age and is maximal from 4 to 10 months. In contrast, no significant modification with age was observed for the activation of B[a]P. It is concluded that the composition of different cytochrome P-450 fractions is modified with age thereby altering the specific ability to convert different promutagens to mutagens.
authors.affiliation: Laboratory of Teratogenesis and Mutagenesis, University of Louvain, Brussels, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Hilali
authors.forename: M C
authors.initials: MC
authors.lastname: Crutzen-Fayt
authors.forename: G B
authors.initials: GB
authors.lastname: Gerber
authors.forename: A
authors.initials: A
authors.lastname: Léonard
mesh_terms: D016604:Aflatoxin B1 / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000873:Anthracenes / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000378:metabolism; D001711:Biotransformation; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D051381:Rats
chemical_list: D000873:Anthracenes; D009153:Mutagens; D001564:Benzo(a)pyrene; C006593:2-anthramine; D016604:Aflatoxin B1