title: Pectinase Aspergillus sp. polygalacturonase: multiplicity, divergence, and structural patterns linking fungal, bacterial, and plant polygalacturonases.
abstract: Nine forms of Aspergillus sp. polygalacturonase were purified from a commercial preparation of pectinase Rohament P using chromatographies and chromatofocusing. Individual forms differ in isoelectric point, and at least five differ in structure; whereas molecular masses and enzymatic properties are largely identical. Four forms with free alpha-amino groups have identical start positions but internal amino acid replacements. Therefore, the multiplicity is derived from true heterogeneities and not from N-terminal truncations. Peptide analysis of the major polygalacturonase reveals large variations toward the enzyme from other Aspergillus species (72-75% residue differences, depending on species) but additional similarities with the enzyme from bacterial and plant sources (only 66-71% residue differences toward the Erwinia, tomato, and peach enzymes). Combined with previous data, these facts show polygalacturonase to exhibit extensive multiplicity and much variability, but also unexpected similarities between distantly related forms with conserved functional properties.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
authors.forename: E
authors.initials: E
authors.lastname: Stratilová
authors.forename: O
authors.initials: O
authors.lastname: Markovic
authors.forename: D
authors.initials: D
authors.lastname: Skrovinová
authors.forename: L
authors.initials: L
authors.lastname: Rexová-Benková
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology*; D001419:Bacteria / Q000201:enzymology; D005658:Fungi / Q000201:enzymology; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology; D011096:Polygalacturonase / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D011096:Polygalacturonase