Transcriptional activation by the adenovirus larger E1a product is mediated by members of the cellular transcription factor ATF family which can directly associate with E1a.
We recently isolated three cDNA clones encoding closely related proteins (ATFa1, ATFa2, and ATFa3) that belong to the activating transcription factor-cyclic AMP-responsive element family of cellular transcription factors. Using cotransfection experiments, we showed that these proteins mediate the transcriptional activation induced by the adenovirus E1a 13S mRNA gene product and that the zinc-binding domains present in both E1a conserved region 3 and the most N-terminal portion of the ATFa proteins play crucial roles in this activity. Reciprocal coimmunoprecipitation experiments demonstrated direct interactions between these proteins. Neither the conserved region 3 domain of E1a nor the N-terminal metal-binding element of ATFa is essential for these interactions. The simultaneous alteration of both the N-terminal and the C-terminal domains of ATFa abolished E1a binding, while either mutation alone failed to impair these interactions.
Chatton
B
B
Laboratoire de Génétique Moléculaire des Eucaryotes, Unité 184 de Biologie Moléculaire et de Génie Génétique, Institut National de la Santé et de la Recherche Médicale, Strasbourg, France.
Bocco
J L
JL
Gaire
M
M
Hauss
C
C
Reimund
B
B
Goetz
J
J
Kedinger
C
C
D051696:Activating Transcription Factors; D017500:Adenovirus E1A Proteins / Q000502:physiology*; D000260:Adenoviruses, Human / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001798:Blood Proteins / Q000502:physiology*; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation; D016335:Zinc Fingers
D051696:Activating Transcription Factors; D017500:Adenovirus E1A Proteins; D001798:Blood Proteins; D014157:Transcription Factors; D004247:DNA