Expression of the colony-stimulating factor 1 receptor in B lymphocytes.
The FMS proto-oncogene encodes for the colony-stimulating factor 1 receptor (CSF-1R), whose expression within the haematopoietic system has previously been thought to be restricted to cells of the mononuclear phagocyte lineage. We have studied the expression of the CSF-1R in peripheral blood mononuclear cells by indirect immunofluorescence and flow cytometry. FMS expression was detected on both monocytes and B lymphocytes from all samples analysed, including 14 haematologically normal individuals and 31 patients (23 in remission following cytotoxic therapy for lymphoma, six with B-cell chronic lymphocytic leukaemia and two with chronic myelomonocytic leukaemia). The level of FMS expression on B lymphocytes was lower than the level of expression detected on monocytes isolated from the same sample. FMS mRNA expression in B lymphocytes has been confirmed by a reverse transcription-polymerase chain reaction (RT-PCR)-based technique and Northern blot analysis. Thus, FMS may play a role in the normal function of B lymphocytes and, because of its potential oncogenic activity, may contribute to the pathogenesis of malignancies of this cell type.
Baker
A H
AH
Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Ridge
S A
SA
Hoy
T
T
Cachia
P G
PG
Culligan
D
D
Baines
P
P
Whittaker
J A
JA
Jacobs
A
A
Padua
R A
RA
D001402:B-Lymphocytes / Q000737:chemistry*; D001483:Base Sequence; D015870:Gene Expression; D016236:Genes, fms; D006801:Humans; D008223:Lymphoma / Q000378:metabolism; D008969:Molecular Sequence Data; D009000:Monocytes / Q000737:chemistry; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000032:analysis; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis*
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D016186:Receptor, Macrophage Colony-Stimulating Factor