Differential persistence, immunogenicity and protective capacity of temperature-sensitive mutants of Salmonella enteritidis after oral or intragastric administration to mice.
The persistence of Salmonella enteritidis temperature-sensitive (ts) mutants of different phenotypes in Peyer's patches (PP) and the spleen, and their immunogenicity after intragastric (i.g.) and peroral (p.o.) administration to mice was investigated. After p.o. administration the ts mutant C/2/2 colonized PP, but was not recovered from the spleen. After i.g. administration the ts mutant E/1/3 colonized both the spleen and PP for at least 2 weeks. Mutant C/2/2 persisted in PP up to 8 days but was not found in the spleen. Mutant H/2/26, although it poorly colonized the PP, was recovered from the spleen up to day 15 after i.g. administration. Immunization with E/1/3 by either the i.g. or the p.o. routes protected mice from challenge with 100 LD50 of the virulent wild-type (wt) strain. Immunization with either C/2/2 or H/2/26 did not confer protection. The three ts mutants induced the production of local IgA after i.g. administration regardless of their protective capacity.
Gherardi
M M
MM
Departamento de Microbiología Aplicada, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
García
V E
VE
Brizzio
V
V
Sordelli
D O
DO
Cerquetti
M C
MC
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D007114:Immunization; D051379:Mice; D009154:Mutation; D012477:Salmonella enteritidis / Q000276:immunology*; D013696:Temperature
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines