Production of mucoid exopolysaccharide during development of Pseudomonas aeruginosa biofilms.
Production of mucoid exopolysaccharide by planktonic, chemostat-derived, and adherent Pseudomonas aeruginosa 579 bacteria was separately monitored for 7 days by using a lacZ-algD promoter-reporter gene and assays of total carbohydrate and metabolic activity. Mucoid exopolysaccharide production was transiently elevated following adherence but declined to planktonic levels by day 7.
Hoyle
B D
BD
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Williams
L J
LJ
Costerton
J W
JW
D004579:Electron Transport; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D010196:Pancreatic Elastase / Q000096:biosynthesis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis
D006025:Glycosaminoglycans; D011135:Polysaccharides, Bacterial; C046129:mucoid exopolysaccharide (P aeruginosa); D001616:beta-Galactosidase; D010196:Pancreatic Elastase