Crystallization and preliminary crystallographic characterization of the copper-containing amine oxidase from pea seedlings.
The copper-containing amine oxidase from pea seedlings has been crystallized using lithium sulfate as precipitant at pH 5.2. The unit cell is orthorhombic, space group P2(1)2(1)2(1), with dimensions a = 89.3 A, b = 113.4 A, c = 199.0 A. The mass of the asymmetric unit is 131(+/- 13) kDa, consistent with independent evidence that the molecule has two approximately 66 kDa subunits. The crystals diffract to 2.5 A in a synchrotron X-ray beam.
Vignevich
V
V
Department of Inorganic Chemistry, University of Sydney, N.S.W., Australia.
Dooley
D M
DM
Guss
J M
JM
Harvey
I
I
McGuirl
M A
MA
Freeman
H C
HC
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry*; D003460:Crystallization; D007887:Fabaceae / Q000201:enzymology*; D010946:Plants, Medicinal; D014961:X-Ray Diffraction
D006631:Amine Oxidase (Copper-Containing)