Molecular analysis of a flagellar core protein gene of Serpulina (Treponema) hyodysenteriae.
The flaB2 gene encoding a protein located in the core of the periplasmic flagella of Serpulina hyodysenteriae was cloned and sequenced. The FlaB2 protein consists of 285 amino acids and has a calculated molecular mass of 31.1 kDa. Southern blot analysis indicated that at least one, and possibly two genes related to flaB2 are present in the genome of S. hyodysenteriae. Comparison of the amino acid sequence of FlaB2 to sequences present in data banks showed significant similarity with the core flagellins of other spirochaetes, in particular with a FlaB2 protein from Treponema phagedenis.
Koopman
M B
MB
Department of Bacteriology, School of Veterinary Medicine, University of Utrecht, The Netherlands.
Baats
E
E
de Leeuw
O S
OS
van der Zeijst
B A
BA
Kusters
J G
JG
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D017401:Brachyspira hyodysenteriae / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005408:Flagellin; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005408:Flagellin