Germline deletion in a neurofibromatosis type 2 kindred inactivates the NF2 gene and a candidate meningioma locus.
Neurofibromatosis type 2 (NF2) is an autosomal dominant disease which predisposes to the development of schwannomas, meningiomas, ependymomas, and juvenile cataracts. The NF2 gene (NF2) has recently been isolated and maps to chromosome 22q12 between the loci D22S212 and D22S32. Deletion studies in sporadic and NF2 associated schwannomas and meningiomas, and the presence of inactivating mutations in NF2 in patients suggest that it acts as a tumor suppressor gene. A candidate meningioma gene (MEN) has also been isolated from the same interval. A new highly polymorphic (CA)n marker, D22S268, which maps very near to NF2, has allowed us to identify a kindred with three living affected individuals, where the disease is presumably caused by a large germline deletion. Fluorescence in situ hybridization and pulsed field gel electrophoresis confirm the presence of a 700kb deletion which includes the neurofilament heavy chain subunit gene locus (NEFH), D22S268, NF2 and the putative MEN gene. The absence of meningiomas in this pedigree raises doubts as to the existence of a separate MEN locus in this region. These results support the hypothesis that NF2 results from the inactivation of a tumor suppressor gene on chromosome 22q.
Sanson
M
M
Center for Research in Neuroscience, McGill University, Montreal, Canada.
Marineau
C
C
Desmaze
C
C
Lutchman
M
M
Ruttledge
M
M
Baron
C
C
Narod
S
S
Delattre
O
O
Lenoir
G
G
Thomas
G
G
D002478:Cells, Cultured; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003360:Cosmids; D005260:Female; D017353:Gene Deletion; D016515:Genes, Neurofibromatosis 2; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D009442:Neurilemmoma / Q000235:genetics*; D016518:Neurofibromatosis 2 / Q000235:genetics*; D016900:Neurofilament Proteins / Q000235:genetics; D015345:Oligonucleotide Probes; D010375:Pedigree; D015183:Restriction Mapping
D005819:Genetic Markers; D016900:Neurofilament Proteins; D015345:Oligonucleotide Probes