The growth of cell culture-attenuated rinderpest virus in bovine lymphoblasts with B cell, CD4+ and CD8+ alpha/beta T cell and gamma/delta T cell phenotypes.
Cloned bovine lymphoblastoid cell lines, transformed by the protozoan parasite Theileria parva were infected with cell culture-attenuated rinderpest virus vaccine. The virus grew readily in lymphoid B cells, CD4+ and CD8+ alpha/beta T cells and gamma/delta T cells producing new infectivity, viral antigens, c.p.e. and total cell death. There did not appear to be a predilection for any particular phenotype of lymphoblast. The results imply that if the vaccine causes immunosuppression, it could do so through a variety of mechanisms.
Rossiter
P B
PB
Kenya Agricultural Research Institute, National Veterinary Research Centre, Muguga, Kikuyu.
Herniman
K A
KA
Gumm
I D
ID
Morrison
W I
WI
D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D016130:Immunophenotyping; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D012302:Rinderpest virus / Q000254:growth & development*; D016176:T-Lymphocyte Subsets / Q000382:microbiology*
D003470:Culture Media; D011948:Receptors, Antigen, T-Cell