Brain calcium metabolism in hypoglycemic coma.
The present experiments were designed to provide information on brain calcium metabolism during hypoglycemic coma. We specifically wished to evaluate changes in extracellular calcium concentration (Ca2+e) during prolonged hypoglycemic coma and recovery and to assess whether Ca2+e falls to similar values during hypoglycemia and ischemia. To that end, Ca2+e and K+e in neocortical tissue were recorded by ion-sensitive microelectrodes during hypoglycemic coma of 30 min duration and during 15 min of recovery. Cardiac arrest ischemia was induced either at the end of the period of hypoglycemia or after 15 min of recovery. Hypoglycemic coma, as reflected by a DC potential shift and by cellular release of K+, was accompanied by a sustained decrease in Ca2+e from approximately 1.2 to approximately 0.02 mM, i.e., to approximately 1% of control. Infusion of glucose was followed by a biphasic recovery of Ca2+e, starting within 2 min of infusion. During the first phase, completed within the initial 3-4 min, Ca2+e rose to about 25% of control. During the second phase, Ca2+e slowly increased toward normal within 25-30 min. Ischemia, when induced at the end of the period of hypoglycemia, was accompanied by a rise in Ca2+e to about 0.1 mM, i.e., about 10% of control. A similar value was recorded when ischemia was induced after 15 min of recovery following a 30-min hypoglycemic coma. Although the present results do not give information on Ca2+i during hypoglycemic coma, it is tempting to conclude that partial preservation of the nucleoside triphosphate stores, and absence of acidosis, allow some binding and sequestration of the calcium entering the cell.(ABSTRACT TRUNCATED AT 250 WORDS)
Kristián
T
T
Laboratory of Experimental Brain Research, Lund University Hospital, Sweden.
Gidö
G
G
Siesjö
B K
BK
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D002545:Brain Ischemia / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D003128:Coma / Q000209:etiology / Q000378:metabolism*; D004567:Electrodes, Implanted; D004569:Electroencephalography; D007003:Hypoglycemia / Q000150:complications / Q000378:metabolism*; D007700:Kinetics; D008839:Microelectrodes; D051381:Rats; D017208:Rats, Wistar
D002118:Calcium