title: Effects of colchicine on the induction of ornithine decarboxylase and its gene expression by the phorbol ester tumour promoter.
abstract: The activity and gene expression of ornithine decarboxylase (ODC, an indicator of tumour promotion) were induced by the phorbol ester tumour promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), in mouse skin. In the present study, the effect of colchicine, a microtubule-disrupting agent, on ODC activity and its gene expression were investigated. On administration of colchicine (100 micrograms) intraperitoneally 1.5 h before TPA treatment, ODC activity and ODC mRNA levels stimulated by TPA were suppressed to about 52 and 64%, respectively. These results suggest the involvement of a microtubule or colchicine-sensitive substrate in the signal transduction system for gene expression.
authors.affiliation: Department of Dermatology, Osaka City University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kono
authors.forename: S
authors.initials: S
authors.lastname: Taniguchi
authors.forename: N
authors.initials: N
authors.lastname: Mizuno
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
authors.forename: N
authors.initials: N
authors.lastname: Maekawa
authors.forename: T
authors.initials: T
authors.lastname: Hisa
authors.forename: M
authors.initials: M
authors.lastname: Ishii
authors.forename: S
authors.initials: S
authors.lastname: Otani
authors.forename: T
authors.initials: T
authors.lastname: Hamada
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D003078:Colchicine / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D051379:Mice; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger; D012867:Skin / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate; D003078:Colchicine