title: Liposomes as a carrier for mannophosphoinositide antigens of mycobacteria.
abstract: Phosphatidylcholine liposomes have been used as carriers of mannophosphoinositides (PIMs) of mycobacteria to examine their immunological properties. PIMs incorporated in egg phosphatidylcholine (EPC) liposomes elicited both humoral and cell-mediated immune responses in mice. Addition of cholesterol at 43 mole% to PC enhanced the immune responses while the reverse was observed with EPC liposomes bearing negative or positive charge. Liposomes made of dioleoylphosphatidylcholine (DOPC) with cholesterol (43 mole%) proved to be a better immunoadjuvant with this antigen as compared to those made of EPC or dipalmitoylphosphatidylcholine (DPPC). Our results suggest that DOPC liposomes containing cholesterol are better carriers for PIMs-antigen as compared to Freund's incomplete adjuvant.
authors.affiliation: Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh, India.
authors.forename: A P
authors.initials: AP
authors.lastname: Singh
authors.forename: G K
authors.initials: GK
authors.lastname: Khuller
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D004337:Drug Carriers; D005260:Female; D006968:Hypersensitivity, Delayed; D007074:Immunoglobulin G / Q000096:biosynthesis; D008081:Liposomes; D008297:Male; D008560:Membrane Fluidity; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D009161:Mycobacterium / Q000276:immunology*; D010716:Phosphatidylinositols / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D004337:Drug Carriers; D007074:Immunoglobulin G; D008081:Liposomes; D010716:Phosphatidylinositols; C002423:mannophosphoinositide