title: Isolation of pigment-free bulk lipids from thylakoids.
abstract: Lipids from spinach thylakoids were extracted with chloroform/methanol and separated from pigments in a single chromatographic step run at 5 degrees C using silicic acid adjusted to pH 8. The isolated lipid fraction contained essentially the same amounts of individual lipids as in the initial extract. It contained less than 0.1% of the initial chlorophylls and carotenoids.
authors.affiliation: Plant Biochemistry, University of Lund, Sweden.
authors.forename: C E
authors.initials: CE
authors.lastname: Bratt
authors.forename: H E
authors.initials: HE
authors.lastname: Akerlund
mesh_terms: D002845:Chromatography / Q000379:methods; D008055:Lipids / Q000302:isolation & purification*; D010860:Pigments, Biological / Q000302:isolation & purification; D010944:Plants / Q000737:chemistry*; D012824:Silicic Acid; D013696:Temperature
chemical_list: D008055:Lipids; D010860:Pigments, Biological; D012824:Silicic Acid