Adsorption and uptake of the alkyllysophospholipid ET-18-OCH3 by HL-60 cells during induction of differentiation by dimethylsulfoxide.
The human leukemic cell line HL-60 is highly sensitive to the antineoplastic agent alkyllysophospholipid, 1-octadecyl-2-methyl-sn-glycerol-3-phosphocholine (ET-18-OCH3). We investigated the adsorption and uptake of radiolabeled ET-18-OCH3 in undifferentiated HL-60 cells and during differentiation to granulocytes induced by dimethylsulfoxide. HL-60 cells become less sensitive to the cytotoxic action of ET-18-OCH3 during differentiation. The decrease in sensitivity is correlated with a decrease in both adsorption and uptake of [3H]ET-18-OCH3 during differentiation. Binding studies revealed that the binding of ET-18-OCH3 to both undifferentiated and differentiated HL-60 cells is non-saturable which renders the existence of a specific binding place highly unlikely.
Heesbeen
E C
EC
Department of Hematology, University Hospital Utrecht, The Netherlands.
Verdonck
L F
LF
Haagmans
M
M
van Heugten
H G
HG
Staal
G E
GE
Rijksen
G
G
D000327:Adsorption; D001692:Biological Transport; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D006801:Humans; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D010742:Phospholipid Ethers / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D010742:Phospholipid Ethers; C026659:edelfosine