Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii.
We have constructed a plasmid to express and secrete dextranase in the oral bacterium Streptococcus gordonii. The dextranase gene from Arthrobacter sp. strain CB-8 was linked to a promoter and a DNA sequence encoding the signal peptide of Streptococcus downei glucosyltransferase I (gtfI) followed by the Escherichia coli rrnBt1t2 terminator and inserted in the shuttle vector pVA838. S. gordonii transformed with this plasmid (pMNK-4) expressed and secreted mature Arthrobacter dextranase. The transformant was found to repress the firm adherence of water-insoluble glucan in a coculture experiment with cariogenic bacteria, Streptococcus sobrinus, in the presence of sucrose. Such genetically engineered oral bacteria could provide a therapy to prevent dental caries.
Kubo
S
S
First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Osaka University, Japan.
Kubota
H
H
Ohnishi
Y
Y
Morita
T
T
Matsuya
T
T
Matsushiro
A
A
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology*; D001422:Bacterial Adhesion; D001483:Base Sequence; D003001:Cloning, Molecular; D003910:Dextranase / Q000235:genetics* / Q000378:metabolism; D005936:Glucans / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics; D016986:Streptococcus sobrinus / Q000472:pathogenicity; D014170:Transformation, Genetic
D005936:Glucans; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D003910:Dextranase