Protein tyrosine kinases couple the interleukin-2 receptor to p21ras.
The T cell growth factor interleukin-2 (IL-2) induces p21ras activation in T lymphocytes. We have previously shown that a protein kinase C (PKC)-mediated pathway for p21ras regulation exists in T cells and that the IL-2 receptor (IL-2R) can couple to p21ras independently of the presence of the PKC pathway for p21ras regulation. Our data show that in conditions where cellular protein tyrosine kinases (PTK) were efficiently down-regulated by pretreatment with the specific PTK inhibitor herbimycin, the IL-2-induced activation of p21ras was blocked. Herbimycin did not inhibit the PKC-mediated pathway for p21ras regulation. Thus, the data indicate that PTK are involved in the coupling of the IL-2R to p21ras.
Izquierdo
M
M
Lymphocyte Activation Laboratory, Imperial Cancer Research Fund Laboratories, London, GB.
Cantrell
D A
DA
D016227:Benzoquinones; D002478:Cells, Cultured; D015536:Down-Regulation; D004789:Enzyme Activation; D020690:GTPase-Activating Proteins; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D047029:Lactams, Macrocyclic; D008213:Lymphocyte Activation; D010766:Phosphorylation; D011493:Protein Kinase C / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000502:physiology*; D011506:Proteins / Q000502:physiology; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D017828:Rifabutin / Q000031:analogs & derivatives; D020703:ras GTPase-Activating Proteins
D016227:Benzoquinones; D020690:GTPase-Activating Proteins; D007376:Interleukin-2; D047029:Lactams, Macrocyclic; D011506:Proteins; D011809:Quinones; D015375:Receptors, Interleukin-2; D020703:ras GTPase-Activating Proteins; D017828:Rifabutin; C020754:herbimycin; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)