title: A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS38.
abstract: A biosurfactant termed arthrofactin produced by Arthrobacter species strain MIS38 was purified and chemically characterized as 3-hydroxydecanoyl-D-leucyl-D-asparagyl-D-threonyl-D- leucyl-D-leucyl-D-seryl-L-leucyl-D-seryl-L-isoleucyl-L-isoleucyl-L-as paragyl lactone. Surface activity of arthrofactin was examined, with surfactin as a control. Critical micelle concentration values of arthrofactin and surfactin were around 1.0 x 10(-5) M and 7.0 x 10(-5) M at 25 degrees C, respectively. Arthrofactin was found to be five to seven times more effective than surfactin. The minimum surface tension value of arthrofactin was 24 mN/m at a concentration higher than the critical micelle concentration. According to the oil displacement assay, arthrofactin was a better oil remover than synthetic surfactants, such as Triton X-100 and sodium dodecyl sulfate. Arthrofactin is one of the most effective lipopeptide biosurfactants.
authors.affiliation: Department of Biotechnology, Osaka University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Morikawa
authors.forename: H
authors.initials: H
authors.lastname: Daido
authors.forename: T
authors.initials: T
authors.lastname: Takao
authors.forename: S
authors.initials: S
authors.lastname: Murata
authors.forename: Y
authors.initials: Y
authors.lastname: Shimonishi
authors.forename: T
authors.initials: T
authors.lastname: Imanaka
mesh_terms: D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D055666:Lipopeptides; D008074:Lipoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D012995:Solubility; D013055:Spectrophotometry, Infrared; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D055666:Lipopeptides; D008074:Lipoproteins; D009842:Oligopeptides; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C083714:arthrofactin; C009365:surfactin peptide