H1 receptor mediates inositol phosphates response to histamine in gastric smooth muscle of guinea pigs.
The effect of histamine on [3H]-inositol phosphates (IPs) formation was investigated with [3H]-inositol-labeled gastric smooth-muscle cells in guinea pigs. Histamine (10(-5) M) increased the formation of [3H]-IPs in the muscle cells. The increase was significantly inhibited by pyrilamine (10(-5) M) but not by cimetidine (10(-5) M). The contractile response to histamine was also completely inhibited by pyrilamine but not by cimetidine. Phorbol ester 12-myristate 13-acetate (10 microM) significantly inhibited histamine-stimulated [3H]-IPs formation by 56%, whereas forskolin (10 microM) decreased it by 18%. This study demonstrates that the response of [3H]-IPs formation and contraction to histamine is mediated through H1 receptor, and the formation of [3H]-IPs is negatively regulated by protein kinase C in gastric smooth muscle of guinea pigs.
Sim
S S
SS
Dept. of Physiology, Catholic University Medical College, Seoul, South Korea.
Jo
Y H
YH
Hahn
S J
SJ
Yoon
S H
SH
Rhie
D J
DJ
Kim
M S
MS
D000818:Animals; D002478:Cells, Cultured; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000096:biosynthesis*; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D011494:Protein Kinases / Q000502:physiology; D011969:Receptors, Histamine H1 / Q000502:physiology*; D013270:Stomach / Q000502:physiology*
D007295:Inositol Phosphates; D011969:Receptors, Histamine H1; D006632:Histamine; D011494:Protein Kinases