L-DNAs as potential antimessenger oligonucleotides: a reassessment.
Unnatural L-2'-deoxyribonucleosides L-T, L-dC, L-dA and L-dG were prepared from L-arabinose and assembled, by solution or solid phase synthesis, to give L-oligonucleotides (L-DNAs), which contain all four natural bases. The affinity of these modified oligomers for complementary D-ribo- and D-deoxyribo-oligomers was studied with NMR, UV and CD spectroscopies and mobility shift assay on native PAGE. All experimental results indicate that L-DNAs do not, in general, recognize single-stranded, natural DNA and RNA. Hence, contrary to previous suggestions, it is not possible to envisage their use as wide scope antimessenger agents in the selective control of gene expression.
Garbesi
A
A
Istituto ICoCEA-CNR, Ozzano Emilia (BO), Italy.
Capobianco
M L
ML
Colonna
F P
FP
Tondelli
L
L
Arcamone
F
F
Manzini
G
G
Hilbers
C W
CW
Aelen
J M
JM
Blommers
M J
MJ
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D016373:DNA, Antisense / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D018075:RNA, Complementary / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D016373:DNA, Antisense; D009838:Oligodeoxyribonucleotides; D018075:RNA, Complementary; D004247:DNA