Detection of DNA damage-recognition proteins using the band-shift assay and southwestern hybridization.
We describe electrophoresis and biochemical conditions that allow detection of damaged DNA-binding proteins in cell extracts. In addition, we present an overview of the damage-recognition DNA-binding proteins from eukaryotic cells and discuss their hypothetical role in DNA repair.
Protić
M
M
Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Levine
A S
AS
D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D015153:Blotting, Western; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000032:analysis*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D014157:Transcription Factors / Q000032:analysis
D004268:DNA-Binding Proteins; D014157:Transcription Factors