Application of the mini-mu phage for the isolation of lac transcriptional fusions in Bacillus subtilis genes.
A cassette containing a selectable cat gene and the lacZ gene without its own promoter has been incorporated into the mini-Mu bacteriophage genome. This mini-Mu derivative, referred to as mMu-Bs, can be used in Escherichia coli for the generation of lacZ transcriptional fusions to Bacillus subtilis genes cloned into plasmids. The resultant fusions can be analyzed in B. subtilis either as multicopy plasmids or as a single copy integrated via a Campbell-like recombination into the wild-type locus of the cloned fragment.
Gardiol
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Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
Gramajo
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Hirschbein
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de Mendoza
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D001412:Bacillus subtilis / Q000235:genetics*; D010583:Bacteriophage mu / Q000235:genetics*; D015139:Blotting, Southern; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D005798:Genes, Bacterial; D007763:Lac Operon; D016296:Mutagenesis; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D015500:Chloramphenicol O-Acetyltransferase