Drugs that suppress hepatic fat synthesis in starved-refed BHE/cdb rats also have an effect on muscle protein synthesis.
Simultaneous lipogenesis and protein synthesis as influenced by LY79771, testosterone, or dehydroepiandrosterone in starved/refed rats were studied. Starved-refed BHE/cdb rats were injected with one of these compounds during the 2-day refeed period. Hepatic de novo fatty acid synthesis using tritium incorporation into fatty acids and protein synthesis using [14C]phenylalanine incorporation into hepatic and muscle protein were determined. Hepatic lipogenesis was decreased by all three drugs and these drugs had a differential effect on protein synthesis. We did not observe a corresponding increase in protein synthesis in the liver when fat synthesis was decreased, but we did observe a corresponding increase in muscle protein synthesis. We concluded that in the acute hyperlipogenic state induced by starvation/refeeding, these drugs induced a reciprocal increase in muscle protein synthesis along with a suppression of fatty acid synthesis.
Parente
J A
JA
Department of Foods and Nutrition, University of Georgia, Athens 30602.
Berdanier
C D
CD
D015078:2-Hydroxyphenethylamine / Q000031:analogs & derivatives / Q000494:pharmacology; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D051381:Rats; D011922:Rats, Mutant Strains; D013217:Starvation; D013739:Testosterone / Q000494:pharmacology
D008055:Lipids; D009124:Muscle Proteins; D013739:Testosterone; D003687:Dehydroepiandrosterone; D015078:2-Hydroxyphenethylamine; C033231:LY 79771