title: Genome analysis of adenovirus 4 isolated over a six year period.
abstract: Genome analysis was carried out on 74 adenovirus 4 (Ad4) isolates from patients in Manchester between 1984 and 1989. Most of the isolates were associated with conjunctivitis. Of the 74 isolates studied, 51 were Ad4a and 10 were Ad4p (the prototype strain). The remaining isolates consisted of two new genome types we have designated Ad4a2 (10 isolates) and Ad4a3 (3 isolates). Most of the genome types co-circulated during the period of study. The Bst E II and Xho I restriction maps of the new variants are presented and compared with those of Ad4p. We are unable to associate genome types with particular clinical presentations.
authors.affiliation: North Manchester Regional Virus Laboratory, Booth Hall Children's Hospital, England.
authors.forename: R J
authors.initials: RJ
authors.lastname: Cooper
authors.forename: A S
authors.initials: AS
authors.lastname: Bailey
authors.forename: R
authors.initials: R
authors.lastname: Killough
authors.forename: S J
authors.initials: SJ
authors.lastname: Richmond
mesh_terms: D000258:Adenovirus Infections, Human / Q000382:microbiology*; D000260:Adenoviruses, Human / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003231:Conjunctivitis / Q000382:microbiology*; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D008875:Middle Aged; D012141:Respiratory Tract Infections / Q000382:microbiology*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C032823:GGTNACC-specific type II deoxyribonucleases