Toxicokinetics of nickel in mice studied with the gamma-emitting isotope 57Ni.
The gamma-emitting isotope 57Ni was generated in a cyclotron to allow whole-body counting of laboratory animals dosed with nickel. 57NiCl2 was administered either orally by gastric intubation or by intraperitoneal injection to groups of mice in doses equivalent to the average human daily dietary nickel intake per mass unit. When given orally, the whole-body retention (WBR) was 0.02-0.36% of the administered dose at 45-75 hr. When given intraperitoneally, the WBR was 1-6% at 20-50 hr. After adjustment for the rapid excretion of systemic nickel, the intestinal absorption could be estimated to be 1.7-10%. The relative WBR did not vary with the magnitude of the dose within 0.05-5 mumol Ni/kg given orally or 0.005-0.5 mumol/kg given intraperitoneally. At 8 hr, the tissue concentration was highest in the kidneys, followed by the carcass, lungs, testicles, liver, and the spleen. After 20 hr, the highest concentrations were still found in the kidneys followed by the lungs, the liver, and the carcass. At 20 hr after oral administration, 50-70% of 57Ni retained in the body was within the carcass. The second highest nickel content was found in the kidneys, followed by the liver and lungs. Whereas nickel in the kidneys was rapidly excreted, the elimination from the lungs and liver was relatively slow, thereby, after 40 hr, resulting in a higher nickel content in the liver than that in the kidneys.(ABSTRACT TRUNCATED AT 250 WORDS)
Nielsen
G D
GD
Department of Environmental Medicine, Odense University, Denmark.
Andersen
O
O
Jensen
M
M
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005720:Gamma Rays; D007274:Injections, Intraperitoneal; D007408:Intestinal Absorption; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009532:Nickel / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D011868:Radioisotopes / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity; D014018:Tissue Distribution
D011868:Radioisotopes; D009532:Nickel