title: Measurement of binding kinetics using the gel electrophoresis mobility shift assay.
abstract: The gel electrophoresis mobility shift assay is a technique for the qualitative and quantitative analysis of protein-DNA complexes. The ability to resolve reactants, reaction intermediates and products makes this method particularly well-suited for the measurement of the assembly and dissociation rates of protein-nucleic acid complexes. Here we identify conditions that must be met and variations of the technique that are useful for the measurement of reaction rates.
authors.affiliation: Department of Surgery, Pennsylvania State University College of Medicine, Hershey 17033.
authors.forename: J T
authors.initials: JT
authors.lastname: Gerstle
authors.forename: M G
authors.initials: MG
authors.lastname: Fried
mesh_terms: D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007700:Kinetics; D007763:Lac Operon; D012097:Repressor Proteins / Q000378:metabolism
chemical_list: D002373:Cyclic AMP Receptor Protein; D012097:Repressor Proteins; D004247:DNA