Lysophosphatidylcholine accumulation in ischemic human myocardium.
Lysophosphatidylcholine accumulates in the coronary sinus during pacing-induced myocardial ischemia in humans. This amphiphile accelerates Ca++ flux leading to cell injury in cultured cardiac myocytes, but it is not known whether lysophosphatidylcholine accumulation is injurious to human myocardium. In this study, we measured lysophosphatidylcholine in normal human myocardium obtained during cardiac surgery and exposed to ischemic conditions in vitro. Total lysophosphatidylcholine concentration (sum of lysophosphatidylcholine remaining in tissue and lysophosphatidylcholine released into the buffer) increased from 0.73 +/- 0.08 nmol/mg protein at baseline to 1.83 +/- 0.45 nmol/mg protein after 5 minutes of ischemia (p < 0.001), and was associated with evidence of cell injury (26% depletion of tissue lactate dehydrogenase). Significant lysophosphatidylcholine release into the incubation buffer (0.41 +/- 0.11 nmol/mg protein) also occurred after 5 minutes of ischemia. In contrast, there was no lysophosphatidylcholine accumulation or release and no lactate dehydrogenase depletion in oxygenated and perfused controls. Attenuation of lysophosphatidylcholine accumulation by incubation with lysophospholipase did not prevent cell injury. Lysoplasmalogen was not detected in ischemic tissue. We conclude that lysophosphatidylcholine accumulation is a marker of myocardial ischemia in humans.
Sedlis
S P
SP
Cardiology Division, New York Department of Veterans Affairs Medical Center, NY.
Hom
M
M
Sequeira
J M
JM
Esposito
R
R
D003327:Coronary Disease / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008245:Lysophospholipase / Q000494:pharmacology; D008246:Lysophospholipids / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D012016:Reference Values; D013482:Superoxide Dismutase / Q000494:pharmacology
D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010713:Phosphatidylcholines; D010743:Phospholipids; C021215:lysoplasmalogens; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D008245:Lysophospholipase