Detection of the PML/RAR alpha fusion gene in acute promyelocytic leukemia with a complex translocation involving chromosomes 15, 17, and 18.
We report a case with typical clinical features of acute promyelocytic leukemia (APL) carrying an atypical chromosomal aberration involving chromosomes 15, 17, and 18. Molecular analysis using Southern blot hybridization and reverse transcriptase-polymerase chain reaction (RT-PCR) proved the creation of the PML/RAR alpha fusion gene in this case. These findings support the notion that this fusion is of crucial importance to leukemogenesis of APL.
Ogawa
S
S
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Mitani
K
K
Sato
Y
Y
Sugimoto
K
K
Toyoshima
H
H
Mano
H
H
Takaku
F
F
Yazaki
Y
Y
Hirai
H
H
D001483:Base Sequence; D015139:Blotting, Southern; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D002887:Chromosomes, Human, Pair 18; D006801:Humans; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D014178:Translocation, Genetic
D018168:Receptors, Retinoic Acid