title: Herpes simplex virus thymidine kinase and specific stages of latency in murine trigeminal ganglia.
abstract: From marker rescue, sequencing, transcript, and latency analyses of the thymidine kinase-negative herpes simplex virus mutant dlsactk and studies using the thymidine kinase inhibitor Ro 31-5140, we infer that the virus-encoded thymidine kinase is required in murine trigeminal ganglia for acute replication and lytic gene expression, for increasing the numbers of cells expressing latency-associated transcripts, and for reactivation from latent infection.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: J G
authors.initials: JG
authors.lastname: Jacobson
authors.forename: K L
authors.initials: KL
authors.lastname: Ruffner
authors.forename: M
authors.initials: M
authors.lastname: Kosz-Vnenchak
authors.forename: C B
authors.initials: CB
authors.lastname: Hwang
authors.forename: K K
authors.initials: KK
authors.lastname: Wobbe
authors.forename: D M
authors.initials: DM
authors.lastname: Knipe
authors.forename: D M
authors.initials: DM
authors.lastname: Coen
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D005814:Genes, Viral; D051379:Mice; D008969:Molecular Sequence Data; D018139:Simplexvirus / Q000201:enzymology / Q000254:growth & development*; D013937:Thymidine Kinase / Q000235:genetics*; D012668:Trigeminal Ganglion / Q000382:microbiology*; D015678:Viral Structural Proteins / Q000235:genetics; D017735:Virus Latency; D014779:Virus Replication
chemical_list: D017931:DNA Primers; D015678:Viral Structural Proteins; D013937:Thymidine Kinase