title: Differential expression of MAGE-1, -2, and -3 messenger RNA in transformed and normal human cell lines.
abstract: The MAGE-1 gene codes for a tumor-specific antigen, MZ2-E, that elicited a cytotoxic T-lymphocyte response in the melanoma patient from whom it was derived. We have developed a simplified method, using polymerase chain reaction amplification of exon 3 followed by restriction enzyme pattern analysis, to distinguish expression of the MAGE-1 gene from MAGE-2 and MAGE-3, other members of this gene family. MAGE-1 mRNA was expressed in 53% of 17 melanoma lines, two of seven Epstein-Barr virus-transformed B-cell lines, and 2 of 5 breast cell lines including a line established form normal breast epithelium. MAGE-1 is not likely to be the common melanoma antigen recognized by the other HLA-A1- or HLA-A2-restricted cytotoxic T-lymphocytes examined in this study, but the fact that it is expressed in about 50% of melanoma cell lines makes it a reasonable target for the immunotherapy of patients bearing HLA-A1.
authors.affiliation: Surgery Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892.
authors.forename: R
authors.initials: R
authors.lastname: Zakut
authors.forename: S L
authors.initials: SL
authors.lastname: Topalian
authors.forename: Y
authors.initials: Y
authors.lastname: Kawakami
authors.forename: M
authors.initials: M
authors.lastname: Mancini
authors.forename: S
authors.initials: S
authors.lastname: Eliyahu
authors.forename: S A
authors.initials: SA
authors.lastname: Rosenberg
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D002461:Cell Line, Transformed; D015870:Gene Expression / Q000235:genetics; D015788:HLA-A1 Antigen / Q000276:immunology; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000276:immunology*; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D015788:HLA-A1 Antigen; D015789:HLA-A2 Antigen; C072011:MAGEA1 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D012333:RNA, Messenger