Inhibition of protein kinase C zeta subspecies blocks the activation of an NF-kappa B-like activity in Xenopus laevis oocytes.
Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of a large variety of cellular genes. However, the mechanism whereby this nuclear factor is activated remains to be determined. In this report, we present evidence that in oocytes from Xenopus laevis, (i) ras p21- and phospholipase C (PLC)-mediated phosphatidylcholine (PC) hydrolysis activates NF-kappa B and (ii) protein kinase C zeta subspecies is involved in the activation of NF-kappa B in response to insulin/ras p21/PC-PLC. Thus, the microinjection of either ras p21 or PC-PLC, or the exposure of oocytes to insulin, promotes a significant translocation to the nucleus of an NF-kappa B-like activity. This effect is not observed when oocytes are incubated with phorbol myristate acetate or progesterone, both of which utilize a ras p21-independent pathway for oocyte activation. These data strongly suggest a critical role of the insulin/ras p21/PC-PLC/protein kinase C zeta pathway in the control of NF-kappa B activation.
Dominguez
I
I
Centro de Biología Molecular UAM-CSIC, Madrid, Spain.
Sanz
L
L
Arenzana-Seisdedos
F
F
Diaz-Meco
M T
MT
Virelizier
J L
JL
Moscat
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D015497:HIV-1 / Q000235:genetics; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000494:pharmacology; D015533:Transcriptional Activation; D014982:Xenopus laevis
D007328:Insulin; D016328:NF-kappa B; D011493:Protein Kinase C; D016283:Proto-Oncogene Proteins p21(ras)