Morphometry of the liver after liver transplantation in the rat: significance of an intact arterial supply.
Two models of orthotopic liver transplantation in the rat currently used are with and without reconstruction of the hepatic artery. The aim of this study was to assess the importance of the arterial blood supply to liver structure after orthotopic liver transplantation with advanced morphometric methods. Orthotopic liver transplantation was performed in male Lewis rats, of which 11 underwent reconstruction of the hepatic artery and 11 did not. A group of untreated controls (n = 5) and a group of animals with hepatic artery ligation (n = 4) were included. Eight weeks after surgery liver tissue was harvested and subjected to systematic random sampling. A point-counting method was used, volume fractions of tissue components were determined. Liver samples from rats that underwent hepatic artery reconstruction had preserved lobular architecture, and estimated bile duct (0.71% +/- 0.33% [S.D.]) and connective tissue (1.89% +/- 0.52%) volumes were not significantly different from those of controls (bile duct, 0.34% +/- 0.17%; connective tissue, 0.70% +/- 0.07%). In contrast, liver samples from rats that did not undergo hepatic artery reconstruction showed bile duct proliferation (7.19% +/- 4.83%; p < 0.05) and an increase in connective tissue volume (7.54% +/- 3.68%; p < 0.05) associated with a decrease in hepatocyte volume (controls, 87.3% +/- 0.3%; rats that underwent arterialization, 85.5% +/- 1.0%; rats that did not undergo arterialization, 73.2% +/- 8.2% [p < 0.05]). Interestingly, hepatic artery ligation had no significant effect on any parameter.(ABSTRACT TRUNCATED AT 250 WORDS)
Zhao
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D
Department of Visceral Surgery, University of Berne, Inselspital, Switzerland.
Zimmermann
A
A
Wheatley
A M
AM
D000818:Animals; D001158:Arteries / Q000503:physiopathology; D001652:Bile Ducts / Q000473:pathology; D002455:Cell Division; D006499:Hepatic Artery; D008026:Ligation; D008099:Liver / Q000473:pathology* / Q000503:physiopathology; D008102:Liver Circulation; D016031:Liver Transplantation; D008297:Male; D009336:Necrosis; D051381:Rats; D011917:Rats, Inbred Lew