title: Fluorescence polarization immunoassay detection of amphetamine, methamphetamine, and illicit amphetamine analogues.
abstract: The Abbott Diagnostics Amphetamine/Methamphetamine II and Amphetamine Class reagents were evaluated on the Abbott TDx for cross-reactivity to amphetamine and methamphetamine stereoisomers, several of their metabolites, and various illicit analogues, including 2-methoxyamphetamine, 4-hydroxymethamphetamine, 2,5-dimethoxy-amphetamine (DMA), 4-bromo-2,5-dimethoxyamphetamine (DOB), 4-bromo-2,5-dimethoxy-beta-phenethylamine (BDMPEA), 3,4,5-trimethoxyamphetamine (TMA), 3,4-methylenedioxy-amphetamine (MDA) N,N-dimethyl-3,4-methylenedioxy-amphetamine, N-hydroxy-3,4-methylenedioxyamphetamine (N-OH MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDEA), 2,5-dimethoxy-4-ethylamphetamine (DOE), 2,5-dimethoxy-4-methylamphetamine (DOM), and mescaline in concentrations ranging from 100 to 100,000 ng/mL. Results demonstrate the utility of this assay for detection of several of the above compounds; unfortunately many are still not detectable. Significant differences were observed between the Amphetamine/Methamphetamine II and Amphetamine Class reagents, particularly regarding their cross-reactivity to over-the-counter medications. Detection of the drugs amphetamine, methamphetamine, and the illicit analogues is not enhanced with the Amphetamine Class reagents, and unless detection of the over-the-counter compounds is of interest, these reagents are a poor choice compared to the Amphetamine/Methamphetamine II reagents. Cross-reactivity of some of the illicit analogues is such that the assay can reliably be used for the routine screening of these compounds.
authors.affiliation: Clinical Investigation Directorate, Wilford Hall Medical Center, Lackland AFB, TX 78236-5300.
authors.forename: J T
authors.initials: JT
authors.lastname: Cody
authors.forename: R
authors.initials: R
authors.lastname: Schwarzhoff
mesh_terms: D000662:Amphetamines / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D002138:Calibration; D003429:Cross Reactions; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D008694:Methamphetamine / Q000032:analysis* / Q000276:immunology; D013237:Stereoisomerism
chemical_list: D000662:Amphetamines; D008694:Methamphetamine