Comparative mapping of 9 human chromosome 22q loci in the laboratory mouse.
We present a comparative map of genes on human chromosome 22q and homologous loci in the mouse genome. Gene order in humans was established using a panel of somatic cell hybrids. Genetic maps spanning homologous segments on three mouse chromosomes were generated using an interspecific backcross. The conserved linkage between human chromosome 22 and mouse chromosome 16 includes two closely linked loci, Comt and IgI-1. The second conserved linkage involves human chromosome 22 and mouse chromosome 11 and contains two genetically and physically linked loci, Lif and Nfh. Finally, conserved synteny involving mouse chromosome 15 and human chromosome 22 spans 30 cM and contains five loci (Acr, Bzrp, Dia-1, Il2rb and Pdgfb). Loci within this conserved synteny have been sublocalized to different portions of human chromosome 22. The order of genes on mouse chromosome 15 and human chromosome 22 provides further evidence for chromosomal rearrangements within the conserved synteny that have occurred since the divergence of lineages leading to mice and humans.
Bućan
M
M
Department of Psychiatry, University of Pennsylvania, Philadelphia 19104.
Gatalica
B
B
Nolan
P
P
Chung
A
A
Leroux
A
A
Grossman
M H
MH
Nadeau
J H
JH
Emanuel
B S
BS
Budarf
M
M
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D017124:Conserved Sequence; D006224:Cricetinae; D003412:Cricetulus; D003433:Crosses, Genetic; D017931:DNA Primers; D015342:DNA Probes; D015321:Gene Rearrangement; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009115:Muridae; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015342:DNA Probes