Avidin attachment to red blood cells via a phospholipid derivative of biotin provides complement-resistant immunoerythrocytes.
Preincubation of red blood cells (RBC) in an aqueous dispersion of biotin-phosphatidylethanolamine (biotin-PE) provides binding sites for avidin on the surface of these cells (up to 5 x 10(5) avidin molecules per cell). Previously we have shown that biotin covalently attached to the surface of RBC by a chemical reaction with biotin N-hydroxysuccinimide ester permits attachment of avidin to these cells, resulting in the activation of the alternative pathway of complement with subsequent cell lysis. However, avidin attached to RBC via biotin-PE did not cause complement activation. This is not due to the stabilizing action of biotin-PE. In contrast, various phospholipids, including biotin-PE, enhance the lysis of RBC induced by hemolytic antibodies via the classical complement pathway. The potential of avidin-coated RBC to act as activators of the complement alternative pathway depends on the method of biotin attachment to RBC. Complement-resistant avidin-coated RBC can specifically bind biotinylated antibodies. These immunoerythrocytes effectively and specifically bind to the antigen-coated surface and are not lysed by complement even in the presence of soluble antigen. These data extend the possible applications of immunoerythrocytes in drug targeting.
Muzykantov
V R
VR
Institute of Experimental Cardiology, National Cardiology Research Center, Moscow, Russian Federation.
Smirnov
M D
MD
Klibanov
A L
AL
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D001360:Avidin / Q000276:immunology* / Q000378:metabolism; D001666:Binding Sites, Antibody; D001710:Biotin / Q000031:analogs & derivatives* / Q000378:metabolism; D003167:Complement Activation; D004306:Dose-Response Relationship, Immunologic; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000276:immunology; D012756:Sheep; D013388:Succinimides / Q000378:metabolism
C072348:N-biotinylphosphatidylethanolamine; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D013388:Succinimides; C033788:biotinyl N-hydroxysuccinimide ester; D001360:Avidin; D001710:Biotin