title: Cytokine-induced inhibition of Plasmodium falciparum erythrocytic growth in vitro.
abstract: The addition of recombinant cytokines to Plasmodium falciparum in vitro cultures retarded the growth of the parasite with the effect of recombinant IL-2 (rIL-2) > interferon-gamma (IFN-gamma) > tumour necrosis factor-beta (TNF-beta). The process was concentration dependent, being greatest at 30,000 U/ml and required a 72-h period of continuous exposure for maximum effect. Growth inhibition, as determined morphologically and radiometrically, was a consequence of defective schizont maturation rather than inhibition of merozoite invasion. It was cumulative and detectable within one erythrocytic (48 h) growth cycle.
authors.affiliation: Department of Haematology, London Hospital Medical College, UK.
authors.forename: A S
authors.initials: AS
authors.lastname: Orago
authors.forename: C A
authors.initials: CA
authors.lastname: Facer
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004912:Erythrocytes / Q000469:parasitology*; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D008233:Lymphotoxin-alpha / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007042:Hypoxanthines; D007376:Interleukin-2; D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins; D005973:Glutamine; D019271:Hypoxanthine; D007371:Interferon-gamma