title: Some properties of lysosomal arylamidase in rat liver.
abstract: Lysosomal arylamidase in rat liver was reassessed. The arylamidase was distinguishable into seven types having different isoelectric points by isoelectric fractionation. All the types hydrolyzed valine beta-naphthylamide most rapidly among amino acid beta-naphthylamides tested, but did not hydrolyze arginine beta-naphthylamide. These enzymes exhibited maximum activities at pH 7.0. They had a molecular weight of 135,000 by gel filtration on a Sephacryl S-200. They were all inhibited by sulfhydryl-blocking reagents and activated by sulfhydryl compounds, indicating to be cysteine proteases.
authors.affiliation: Department of Nutritional Chemistry, Nakamura Gakuen College, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hiroi
authors.forename: S
authors.initials: S
authors.lastname: Toku
mesh_terms: D000626:Aminopeptidases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000378:metabolism; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D003546:Cysteine Endopeptidases / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007526:Isoelectric Point; D007527:Isoenzymes; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D009281:Naphthalenes / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014633:Valine / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D009281:Naphthalenes; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; C072587:2-naphthylamide; D001120:Arginine; D000626:Aminopeptidases; D003546:Cysteine Endopeptidases; D014633:Valine