title: A linear 16 pS Cl- channel in the apical membrane of Caco-2 cells.
abstract: A linear Cl- channel with a mean conductance of 16.3 +/- 0.4 pS (n = 7), as determined for inwardly directed currents in the cell-attached mode, was identified by the patch clamp technique in the apical membrane of Caco-2 cells in the early stage of postconfluential differentiation. This channel exhibited a low spontaneous activity (in 3 out of 137 experiments) and was not activated by strong polarization of the holding potential. After stimulation of the cAMP signal transduction cascade at different levels the 16 pS Cl- channel was activated in 7 out of 67 experiments during continuous cell-attached recording and remained active after excision of the patch. This channel differed markedly from both a linear low conductance Cl- channel and an intermediate conductance, outwardly rectifying (ICOR) Cl- channel, already reported for Caco-2 and other colonic cell lines. Thus the 16 pS channel may represent a further type of apical Cl- channel in colonocytes.
authors.affiliation: Universitätskinderklinik, München, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Huber
authors.forename: D
authors.initials: D
authors.lastname: Költgen
authors.forename: A A
authors.initials: AA
authors.lastname: Roscher
authors.forename: P
authors.initials: P
authors.lastname: Heinz-Erian
mesh_terms: D000230:Adenocarcinoma / Q000503:physiopathology; D002454:Cell Differentiation / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D018118:Chloride Channels / Q000502:physiology*; D003110:Colonic Neoplasms / Q000503:physiopathology; D003470:Culture Media; D000242:Cyclic AMP / Q000502:physiology; D004558:Electric Stimulation; D004594:Electrophysiology; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000502:physiology
chemical_list: D018118:Chloride Channels; D003470:Culture Media; D000242:Cyclic AMP