title: Expression cloning exploiting PCR rescue of transfected genes.
abstract: Expression cloning in eukaryotic cells has allowed the isolation of many genes for which no protein or nucleic acid sequence information was available. In this paper we describe a model methodology for PCR-mediated rescue of transfected genes from eukaryotic cell lines after functional selection. This protocol should allow any readily transfectable cell line to serve as a host for functional selection of cDNA expression libraries.
authors.affiliation: Faculty of Biological Science, University of Tulsa, OK 74104.
authors.forename: M
authors.initials: M
authors.lastname: Brudnak
authors.forename: K S
authors.initials: KS
authors.lastname: Miller
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005069:Evaluation Studies as Topic; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D014162:Transfection
chemical_list: D004247:DNA