title: A tobacco-specific lung carcinogen in the urine of men exposed to cigarette smoke.
abstract: BACKGROUND
  Environmental tobacco smoke has been classified by the Environmental Protection Agency as a carcinogen causally associated with lung cancer in adults, but there have been no reports of lung carcinogens or their metabolites in the body fluids or tissues of nonsmokers exposed to environmental tobacco smoke.
  
  
  METHODS
  Five male nonsmokers were exposed to sidestream cigarette smoke generated by machine smoking of reference cigarettes for 180 minutes on each of two days, six months apart. Sidestream smoke is the smoke that originates from the smoldering end of a cigarette between puffs. Twenty-four-hour urine samples were collected before and after exposure. The urine samples were analyzed for 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its glucuronide, which are metabolites of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a powerful lung carcinogen in rodents. NNAL is also a lung carcinogen in rodents.
  
  
  RESULTS
  The urinary excretion of the metabolites increased after exposure to sidestream smoke in all the men. The mean (+/- SD) amount of NNAL and NNAL glucuronide was significantly higher after exposure than at base line (33.9 +/- 20.0 vs. 8.4 +/- 11.2 ng per 24 hours [127 +/- 74 vs. 31 +/- 41 pmol per day], P < 0.001) and was correlated with urinary cotinine excretion (r = 0.89, P < 0.001). The nicotine concentrations in the air to which the men were exposed were comparable to those in a heavily smoke-polluted bar.
  
  
  CONCLUSIONS
  Nonsmokers exposed to sidestream cigarette smoke take up and metabolize a lung carcinogen, which provides experimental support for the proposal that environmental tobacco smoke can cause lung cancer.
authors.affiliation: American Health Foundation, Valhalla, NY 10595.
authors.forename: S S
authors.initials: SS
authors.lastname: Hecht
authors.forename: S G
authors.initials: SG
authors.lastname: Carmella
authors.forename: S E
authors.initials: SE
authors.lastname: Murphy
authors.forename: S
authors.initials: S
authors.lastname: Akerkar
authors.forename: K D
authors.initials: KD
authors.lastname: Brunnemann
authors.forename: D
authors.initials: D
authors.lastname: Hoffmann
mesh_terms: D000328:Adult; D002273:Carcinogens; D003367:Cotinine / Q000652:urine; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced*; D008297:Male; D008875:Middle Aged; D009602:Nitrosamines / Q000652:urine*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D002273:Carcinogens; D009602:Nitrosamines; D014028:Tobacco Smoke Pollution; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; D003367:Cotinine