Stimulation of nerve growth factor synthesis/secretion in mouse astroglial cells by coenzymes.
We examined the effect of coenzymes such as PQQ, pyrroloquinoline quinone; TOPA, 3-(2,4,5-trihydroxyphenyl)-DL-alanine, and lipoic acid on nerve growth factor (NGF) synthesis in mouse astroglial cells, BALB c/3T3 cells, and WS-1 cells in culture. These coenzymes had a stimulating effect on NGF synthesis without causing cytotoxicity. Especially PQQ showed the strongest activity of promoting NGF synthesis in astroglial cells, whereas lipoic acid had the strongest effect on BALB c/3T3 cells. The activity may not due to the catechol ring or 1,4-benzoquinone ring, but due to the oxidative or reductive activity. These results suggest that these coenzymes may play a role in NGF synthesis and neuronal survival through the stimulating effect of the NGF synthesis in brain and such compounds are good candidates as NGF inducers.
Murase
K
K
Department of Molecular Biology, Gifu Pharmaceutical University, Japan.
Hattori
A
A
Kohno
M
M
Hayashi
K
K
D016475:3T3 Cells; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003067:Coenzymes / Q000494:pharmacology*; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000378:metabolism; D010084:Oxidation-Reduction; D045542:PQQ Cofactor; D015363:Quinolones / Q000494:pharmacology*; D008063:Thioctic Acid / Q000494:pharmacology*
D003067:Coenzymes; D009414:Nerve Growth Factors; D015363:Quinolones; C001388:6-hydroxydopa; D004295:Dihydroxyphenylalanine; D045542:PQQ Cofactor; D008063:Thioctic Acid