Induction of metallothionein in a human astrocytoma cell line by interleukin-1 and heavy metals.
The effects of cytokines and heavy metals on the expression and localization of metallothioneins (MTs) within U373MG astrocytoma cells were analyzed by using indirect immunofluorescence using a monoclonal anti-MT antibody (MT45). IL-1, CdCl2 (50 microM) or ZnCl2 (500 microM) remarkably augmented intracellular MT levels, whereas IL-6 or 10 microM of ZnCl2 showed no inducing activity. From 24 to 48 h after the addition of CdCl2 or IL-1, immunoreactive MTs were found in the cytoplasm and the nucleus. After 72 h, immunoreactive MTs accumulated in a granular form near the cell surfaces in the presence of CdCl2 (50 microM) or IL-1 plus ZnCl2 (10 microM). However, this accumulation was not observed when only IL-1 was added. Thus, Zn2+ facilitated the appearance of the granular form of immunoreactive MTs at a concentration where they do not induce MTs by themselves.
Kikuchi
Y
Y
Department of Microbiology, Hoshi College of Pharmacy, Tokyo, Japan.
Irie
M
M
Kasahara
T
T
Sawada
J
J
Terao
T
T
D001254:Astrocytoma; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D002712:Chlorides / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics; D008670:Metals / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds
D002712:Chlorides; D007375:Interleukin-1; D008670:Metals; D012333:RNA, Messenger; D017967:Zinc Compounds; D002104:Cadmium; C016837:zinc chloride; D008668:Metallothionein; D015032:Zinc; D019256:Cadmium Chloride