Identification of toxigenic Vibrio cholerae from the Argentine outbreak by PCR for ctx A1 and ctx A2-B.
A polymerase chain reaction (PCR) to detect a region of the A1 cholera toxin gene was applied to the identification of 43 Vibrio cholerae strains isolated from the recent outbreak in Argentina. A good correlation was observed between the GM1-enzyme-linked immunosorbent assay (GM1-ELISA) to detect the B subunit of the enterotoxin and PCR. However, a V. cholerae non-01 strain that was negative by the ELISA test, was positive by the PCR assay for the A1 region. A second PCR test to detect the A2-B coding region was developed to solve this case. We propose that routine detection of toxigenic V. cholerae by PCR should include analysis of A2-B coding region or the whole cholera toxin operon.
Varela
P
P
Tecnologia Genetica, Buenos Aires, Argentina.
Rivas
M
M
Binsztein
N
N
Cremona
M L
ML
Herrmann
P
P
Burrone
O
O
Ugalde
R A
RA
Frasch
A C
AC
D001118:Argentina; D001483:Base Sequence; D002771:Cholera / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000145:classification / Q000472:pathogenicity*
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D002772:Cholera Toxin