title: Heterologous gene expression in Campylobacter coli: the use of bacterial luciferase in a promoter probe vector.
abstract: A novel promoter probe plasmid (pSP73) was constructed to allow the analysis of environmentally regulated gene expression in Campylobacter. The vector utilizes the luxAB genes from Xenorhabdus luminescens, which encode a thermostable luciferase, as reporters of gene expression. The utility of this reporter system was demonstrated by placing the expression of luxAB under the transcriptional control of the flaA gene promoter.
authors.affiliation: AFRC Institute of Food Research, Reading Laboratory, UK.
authors.forename: D
authors.initials: D
authors.lastname: Purdy
authors.forename: S F
authors.initials: SF
authors.lastname: Park
mesh_terms: D001483:Base Sequence; D017000:Campylobacter coli / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics*; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D008156:Luciferases