Analyses of origin of synovial cells and repairing mechanisms of arthritis by allogeneic bone marrow transplantation.
MRL/lpr mice spontaneously develop rheumatoid arthritis (RA)-like disease. Recently we have observed that bone marrow transplantation (BMT) with bone graft to recruit donor stromal cells can be used to treat autoimmune diseases (including RA-like lesions) in MRL/lpr mice. In this paper, we characterize the origin of synovial cells with the use of radiation chimeras and elucidate the repairing mechanism of RA by BMT. Type A synoviocytes have been thought to play an important role in the initiation of inflamed synovia, since a large number of Type A synoviocytes have been seen in inflamed synovia of both RA patients and MRL/lpr mice. Using [C57BL/6JJic-bg-->MRL/lpr] chimeric mice, we found Type A synoviocytes to be derived from donor bone marrow cells. They appeared in the inflamed synovia 4 weeks after BMT. However, at this time, the repairing process was not prominent. Serial biopsy studies revealed that newly developed T cells with normal functions play a more crucial role in the treatment of RA in MRL/lpr mice than do Type A synoviocytes.
Kitagawa
H
H
First Department of Pathology, Kansai Medical University, Osaka, Japan.
Toki
J
J
Inaba
M
M
Sugiura
K
K
Ogawa
R
R
Ikehara
S
S
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D001327:Autoimmune Diseases / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D016026:Bone Marrow Transplantation / Q000473:pathology* / Q000502:physiology; D002455:Cell Division; D002478:Cells, Cultured; D007249:Inflammation; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D017452:Receptors, IgG / Q000032:analysis; D013583:Synovial Membrane / Q000473:pathology*; D013997:Time Factors; D014184:Transplantation, Homologous
D017452:Receptors, IgG