Prenylated protein methyltransferase of rat cerebellum is developmentally co-expressed with its substrates.
High levels of prenylated protein methyltransferase are expressed in the developing rat cerebellum and are responsible for methylation of endogenous G-proteins and 50-52 kDa synaptosomal proteins. Enzyme activity in cerebellar synaptosomes of 3 week postnatal rats is 2-fold higher than that found in adult rat cerebellum. A 10-fold rise in activity occurs at the end of the second and during the third postnatal weeks, followed by a subsequent decline. Expression of the enzymes' substrates follows the same pattern. The high methyltransferase activity in 3-week-old cerebellum coincides with the period of granule cell migration and synaptogenesis, suggesting a regulatory role for the enzyme and its substrates in cerebellar ontogenesis.
Paz
A
A
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
Ben-Baruch
G
G
Marciano
D
D
Egozi
Y
Y
Haklai
R
R
Kloog
Y
Y
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000201:enzymology* / Q000254:growth & development; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008745:Methylation; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011496:Protein Methyltransferases / Q000378:metabolism*; D051381:Rats; D012436:S-Adenosylmethionine / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D013574:Synaptosomes / Q000201:enzymology
D009419:Nerve Tissue Proteins; D012436:S-Adenosylmethionine; D011496:Protein Methyltransferases; C056501:protein-S-isoprenylcysteine O-methyltransferase; D019204:GTP-Binding Proteins