Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR.
A total of 241 group A rotavirus-positive stool samples collected from diarrhoeic patients in Thailand between July 1988 and June 1991 were characterized for their serotypes by enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies and by a polymerase chain reaction (PCR). In July 1988-June 1989, serotype 1 was the most prevalent (63.4%), followed by serotype 4 (11.0%) and serotype 2 (8.5%). In July 1989-June 1990, 59.8% were serotype 1, 24.3% were serotype 2, and 6.1% were serotype 3. In contrast, in July 1990-June 1991, serotype 3 was detected in the highest frequency (40.5%), 29.9% were serotype 1, and 27.3% were serotype 2. Thus, a distinct yearly change of serotype distribution of rotavirus in Thailand was observed in the three consecutive years. In particular, it was of note that the prevalence of serotype 3 greatly increased, in contrast to the previous studies in which almost no serotype 3 rotaviruses were detected in the years 1983-8 in Thailand.
Pongsuwanna
Y
Y
Virus Research Institute, Department of Medical Science, Nonthaburi, Thailand.
Taniguchi
K
K
Wakasugi
F
F
Sutivijit
Y
Y
Chiwakul
M
M
Warachit
P
P
Jayavasu
C
C
Urasawa
S
S
D004279:DNA, Viral / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D012703:Serotyping; D013785:Thailand / Q000453:epidemiology
D004279:DNA, Viral