title: UDP-galactose:lactosylceramide alpha-galactosyltransferase activity in human placenta.
abstract: The activity of UDP-Gal:LacCer galactosyltransferase in human placenta was studied by using crude homogenate and Triton CF-54 extract as the source of enzyme. Transfer of galactose to lactosylceramide was optimal in the presence of 0.1% Triton CF-54 and Mn2+ at pH 6.3, and the reaction product was susceptible to alpha-galactosidase.
authors.affiliation: Department of Biochemistry, University of Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Lampio
authors.forename: A
authors.initials: A
authors.lastname: Airaksinen
authors.forename: H
authors.initials: H
authors.lastname: Maaheimo
mesh_terms: D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D003902:Detergents; D004795:Enzyme Stability; D005700:Galactosyltransferases / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology*; D012995:Solubility
chemical_list: D003902:Detergents; D005700:Galactosyltransferases; C045097:UDP-galactose-lactosylceramide alpha 1-4-galactosyltransferase