title: Sequence analysis and expression of the cDNA for the phenol-sulfating form of human liver phenol sulfotransferase.
abstract: A cDNA encoding the human liver phenol-sulfating form of phenol sulfotransferase (P-PST) has been isolated and characterized from a lambda Uni-Zap XR human liver cDNA library. P-PST is the major form of phenol sulfotransferase involved in drug and xenobiotic metabolism in human liver. P-PST is also responsible for the sulfation and activation of minoxidil to its therapeutically active sulfate ester. The full length cDNA, P-PST-1, is 1206 base pairs in length and encodes a 295-amino acid protein with a molecular mass of 34,097 Da. The translation sequence of P-PST-1 is 96% similar to the amino acid sequences of five peptides derived from the purified protein. In vitro transcription and translation of P-PST-1 generated a protein that comigrates with immunoreactive P-PST from human liver. Significant increases in sulfotransferase activity toward two P-PST-specific substrates, minoxidil and 4-nitrophenol, were detected in cytosol prepared from COS-7 cells transfected with P-PST-1 in the expression vector p-SV-SPORT-1. Northern blot analysis of human liver RNA detected a transcript of approximately 1300 nucleotides in length. Characterization of P-PST at the molecular level provides insight into the structure and heterogeneity of this major class of drug-metabolizing enzymes.
authors.affiliation: Department of Pharmacology, University of Alabama, Birmingham 35294.
authors.forename: T W
authors.initials: TW
authors.lastname: Wilborn
authors.forename: K A
authors.initials: KA
authors.lastname: Comer
authors.forename: T P
authors.initials: TP
authors.lastname: Dooley
authors.forename: I M
authors.initials: IM
authors.lastname: Reardon
authors.forename: R L
authors.initials: RL
authors.lastname: Heinrikson
authors.forename: C N
authors.initials: CN
authors.lastname: Falany
mesh_terms: D000595:Amino Acid Sequence; D015239:Arylsulfotransferase / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D004247:DNA; D015239:Arylsulfotransferase