Shrinkage prevention of in vitro demineralized human dentine in transversal microradiography.
The shrinkage of demineralized dentine is a serious problem in the assessment of mineral distributions and mineral changes by means of microradiography. In this article it is shown that a treatment with ethanediol of the thin slices used for transversal microradiography prevents shrinkage for about 1 h. Without this so-called diol treatment, the shrinkage effects in demineralized dentin are about 20% in lesion depth and about 30% in mineral loss (delta Z) after 120-second drying in air. The prevention of shrinkage by diol treatment of dentine lesions is not influenced by the presence of a surface layer.
Ruben
J
J
Laboratory for Materia Technica, State University of Groningen, The Netherlands.
Arends
J
J
D003804:Dentin / Q000737:chemistry* / Q000187:drug effects; D003890:Desiccation; D005026:Ethylene Glycols / Q000494:pharmacology; D016591:Histocytological Preparation Techniques; D006801:Humans; D008852:Microradiography; D017001:Tooth Demineralization / Q000473:pathology
D005026:Ethylene Glycols