Anesthesia inhibits interferon-induced natural killer cell cytotoxicity via induction of CD8+ suppressor cells.
Anesthesia (Avertin, halothane, isoflurane, ether, or ketamine/xylazan) of mice inhibits subsequent stimulation of splenic natural killer cell (NK) cytotoxicity by interferon (IFN) treatment either in vitro and in vivo. The current data demonstrate (a) in vitro depletion of CD8+ cells from mononuclear splenocytes of anesthetized mice restored the ability of NK cells to respond in vitro to IFN stimulation and (b) coincubation of CD8+ splenocytes from anesthetized mice with CD8- splenocytes of naive mice resulted in a significant reduction of the IFN-induced stimulation of NK activity in the coculture. These results suggest that anesthesia induces CD8+ cells that suppress stimulation of NK cytotoxicity by IFN.
Markovic
S N
SN
Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129.
Murasko
D M
DM
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D016827:CD8 Antigens; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D000431:Ethanol / Q000031:analogs & derivatives* / Q000494:pharmacology; D007108:Immune Tolerance / Q000187:drug effects*; D016898:Interferon-alpha / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*
D000777:Anesthetics; D016827:CD8 Antigens; D016898:Interferon-alpha; C062527:tribromoethanol; D000431:Ethanol; D016899:Interferon-beta