Role of lipid peroxidation in impairment of mitochondrial function at complex I by methyl isocyanate treatment of rats in vivo.
The subcutaneous administration of methyl isocyanate (MIC) in 1.0 LD50 dose in rats caused a significant effect on hepatic mitochondrial function only at complex I region of the respiratory chain. MIC administration at 1.0 LD50 dose also resulted in significant increases in malondialdehyde and ferrous ion concentration in liver mitochondria. It is suggested that the augmented lipid peroxidation in hepatic mitochondria, catalyzed by iron, possibly mobilized from intracellular stores leads to the inhibition of enzymes of mitochondrial respiration at complex I region, in vivo, in rats receiving a lethal dose of MIC subcutaneously.
Jeevaratnam
K
K
Division of Pharmacology & Toxicology, Defence Research and Development Establishment, Gwalior, India.
Vidya
S
S
Vaidyanathan
C S
CS
D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects; D007501:Iron / Q000032:analysis; D017953:Isocyanates / Q000633:toxicity*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008930:Mitochondria, Liver / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar
D017953:Isocyanates; D008315:Malondialdehyde; C008461:methyl isocyanate; D007501:Iron; D016660:NAD(P)H Dehydrogenase (Quinone)