title: Intraoperative bone and bone marrow sampling: a simple method for accurate measurement of uptake of radiopharmaceuticals in bone and bone marrow.
abstract: Accurate estimation of bone marrow uptake of radiopharmaceuticals is of crucial importance for accurate whole body dosimetry. In this study, a method for obtaining normal bone marrow and bone during routine surgery without inconvenience to volunteers is suggested and compared to an indirect method. In five volunteers (group 1), 4 MBq 111In-labelled human polyclonal IgG (111In-IgG) was administered 48 h before placement of a total hip prosthesis. After resection of the femoral head and neck, bone marrow was aspirated from the medullary space with a biopsy needle. In five patients, suspected of having infectious disease (group 2), bone marrow uptake was calculated according to a well-accepted method using regions of interest over the lumbar spine, 48 h after injection of 75 MBq 111In-IgG. Bone marrow uptake in group 1 (4.5 +/- 1.3% D kg-1) was significantly lower than that in group 2 (8.5 +/- 2.1% D kg-1) (P < 0.01). Blood and plasma activity did not differ significantly for both groups. This method provides a system for directly and accurately measuring uptake and retention in normal bone marrow and bone of all radiopharmaceuticals at various time points. It is a safe and simple procedure without any discomfort to the patient. Since small amounts of activity are sufficient, the radiation dose to the patient is low.
authors.affiliation: Department of Nuclear Medicine, University Hospital Nijmegen, The Netherlands.
authors.forename: W J
authors.initials: WJ
authors.lastname: Oyen
authors.forename: W C
authors.initials: WC
authors.lastname: Buijs
authors.forename: A
authors.initials: A
authors.lastname: van Kampen
authors.forename: E B
authors.initials: EB
authors.lastname: Koenders
authors.forename: R A
authors.initials: RA
authors.lastname: Claessens
authors.forename: F H
authors.initials: FH
authors.lastname: Corstens
mesh_terms: D000368:Aged; D001853:Bone Marrow / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism*; D005260:Female; D006622:Hip Prosthesis; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007205:Indium Radioisotopes / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D008875:Middle Aged; D016343:Monitoring, Intraoperative
chemical_list: D007074:Immunoglobulin G; D007205:Indium Radioisotopes