Coupling of D2 and D3 dopamine receptors to G-proteins.
Recombinant cell lines expressing D2 and D3 dopamine receptor isoforms have been used to study coupling of these receptors to G-proteins from the effects of GTP to reduce agonist binding affinities in agonist/[3H]spiperone competition experiments. D2(short) expressed in Ltk- cells, D2(long) expressed in Ltk- or CHO cells and D3 expressed in CHO cells all showed coupling to the endogenous G-proteins of the cells. The detailed agonist binding characteristics indicated that there were differences in receptor/G-protein coupling for the same receptor(D2(long)) expressed in two cell types or for different receptors (D2(short), D2(long)) expressed in the same cell type.
Castro
S W
SW
Biological Laboratory, The University, Canterbury, Kent, UK.
Strange
P G
PG
D000818:Animals; D001667:Binding, Competitive; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism*; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D050637:Receptors, Dopamine D3; D013134:Spiperone / Q000378:metabolism
C493595:Drd3 protein, rat; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D013134:Spiperone; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins