title: Synthesis of 12-hydroxyeicosatetraenoic acid by human endometrium and decidua.
abstract: In order to clarify the possible involvement of lipoxygenase metabolites of arachidonic acid (AA) in implantation, monohydroxylic acid production was examined using human endometrium and decidua in early pregnancy. As 14C-AA was mainly converted to 12-hydroxyeicosatetraenoic acid (12-HETE) as well as prostaglandins (PGs) by the microsomal fraction of the endometrium, a subsequent study was carried out on the endometrium (at various stages) and decidua in early pregnancy using tissue incubation and high performance liquid chromatography (HPLC) analysis for 12-HETE. The amounts of 12-HETE released into the culture medium by proliferative and secretory endometrium were 165.1 +/- 123.4 (ng/mg.protein/24 h, mean +/- SD) and 502.3 +/- 127.2, respectively, whereas, decidua produced a smaller amount of 12-HETE that declined as gestation progressed. This lipoxygenase product as well as PGs might have a role in implantation.
authors.affiliation: Department of Obstetrics and Gynaecology, Saitama Medical Centre, Saitama Medical School, Kawagoe, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ihno
authors.forename: O
authors.initials: O
authors.lastname: Ishihara
authors.forename: K
authors.initials: K
authors.lastname: Kinoshita
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D002851:Chromatography, High Pressure Liquid; D003656:Decidua / Q000378:metabolism*; D004717:Endometrium / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D008084:Lipoxygenase / Q000032:analysis
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase