title: Induction of G-bands on Anguilla anguilla chromosomes by the restriction endonucleases HaeIII, HinfI, and MseI.
abstract: Fixed metaphase chromosomes of the eel Anguilla anguilla were treated with the restriction enzymes HaeIII, HinfI, and MseI. All three restriction endonucleases are capable of inducing a G-band-like pattern if the incubation time and enzyme concentration are controlled. The results are discussed in relation to the main factors involved in the appearance of serial G-bands. Chromatin organization, rather than DNA composition, seems to account for the interstitial G-band pattern exhibited by these eel chromosomes.
authors.affiliation: Departamento de Biología Fundamental, Universidad de Santiago de Compostela, Lugo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Viñas
authors.forename: C
authors.initials: C
authors.lastname: Gómez
authors.forename: P
authors.initials: P
authors.lastname: Martínez
authors.forename: L
authors.initials: L
authors.lastname: Sánchez
mesh_terms: D000811:Anguilla; D000818:Animals; D002871:Chromosome Banding; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005260:Female; D007621:Karyotyping; D008297:Male
chemical_list: C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases