Assignment of the nuclear mitotic apparatus protein NuMA gene to human chromosome 11q13.
A monoclonal antibody that was specific for a nuclear matrix protein was obtained and used to screen a human lambda gt11 expression library. Several partial cDNA clones were isolated and sequenced. The sequence for this protein was shown to be identical to that of NuMA, a 236-kDa nuclear mitotic spindle apparatus protein. NuMA has been recently characterized by two independent studies, and is thought to be part of a family of proteins that is required for the completion of mitosis. In this report, the chromosomal localization and copy number of the NuMA gene are analyzed using cDNA clones. High-resolution in situ hybridization reveals a single pair of signals on sister chromatids of human chromosome 11 at band q13. Stringent Southern analysis of human genomic DNA resulted in simple restriction patterns. These results together indicate that the NuMA gene is present as a single copy on human chromosome 11q13.
Sparks
C A
CA
Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655.
Bangs
P L
PL
McNeil
G P
GP
Lawrence
J B
JB
Fey
E G
EG
D000911:Antibodies, Monoclonal / Q000276:immunology; D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C050045:NUMA1 protein, human; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins