title: Ultrastructural identification of exocytosis of granules from human gut eosinophils in vivo.
abstract: Twenty-two percent of 117 biopsies of human intestinal tissues had ultrastructural images of classical regulated secretion from eosinophils in vivo i.e. eosinophil granule extrusion (EGE). Replicate intestinal biopsies that were positive for bacteria had EGE more often than not (p < 0.05); 77% of the isolates were Staphylococci. Some of the intestinal biopsies also had damaged nerves; all that had EGE and damaged enteric nerves also had positive bacterial cultures. The EGE that we observed could not account for all enteric nerve damage, suggesting multifactorial mechanisms for nerve damage in gut tissues. Among the possibilities are release of neurotoxic eosinophil granule proteins by an alternate secretory route, i.e., piecemeal degranulation, direct toxicity of tissue invasive bacteria and/or damaged nerves of unknown etiology such as those that are regularly present in uninvolved tissues of patients with Crohn's disease.
authors.affiliation: Harvard Digestive Disease Center, Beth Israel Hospital, Boston, MA 02215.
authors.forename: A M
authors.initials: AM
authors.lastname: Dvorak
authors.forename: A B
authors.initials: AB
authors.lastname: Onderdonk
authors.forename: R S
authors.initials: RS
authors.lastname: McLeod
authors.forename: R A
authors.initials: RA
authors.lastname: Monahan-Earley
authors.forename: D A
authors.initials: DA
authors.lastname: Antonioli
authors.forename: J
authors.initials: J
authors.lastname: Cullen
authors.forename: J E
authors.initials: JE
authors.lastname: Blair
authors.forename: R
authors.initials: R
authors.lastname: Cisneros
authors.forename: L
authors.initials: L
authors.lastname: Letourneau
authors.forename: E
authors.initials: E
authors.lastname: Morgan
mesh_terms: D001369:Axons / Q000648:ultrastructure; D001706:Biopsy; D015550:Cell Degranulation; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004804:Eosinophils / Q000378:metabolism / Q000648:ultrastructure*; D005089:Exocytosis; D005767:Gastrointestinal Diseases / Q000382:microbiology / Q000473:pathology; D006801:Humans; D007422:Intestines / Q000294:innervation / Q000382:microbiology / Q000648:ultrastructure*; D013210:Staphylococcus / Q000302:isolation & purification