Evidence for cis-trans isomerization of a double bond in the fatty acids of the psychrophilic bacterium Vibrio sp. strain ABE-1.
Vibrio sp. strain ABE-1 was grown in a medium that contained as its stable isotope tracer either [2,2-2H2]cis-9-hexadecenoic or [2,2-2H2]trans-9-hexadecenoic acid. Gas chromatographic-mass spectrometric analysis of the cis-9-hexadecenoic and trans-9-hexadecenoic acid fractions from the cells revealed the formation of an intracellularly isomerized 2,2-2H2-fatty acid which differed from the tracer only in the geometrical configuration of the double bond. This observation shows that cis-trans isomerization without a shift in double-bond position between these two geometric hexadecenoic acid isomers can occur in the cells.
Morita
N
N
Department of Biology, Faculty of Science, Hokkaido University, Sapporo, Japan.
Shibahara
A
A
Yamamoto
K
K
Shinkai
K
K
Kajimoto
G
G
Okuyama
H
H
D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D013058:Mass Spectrometry; D013237:Stereoisomerism; D014733:Vibrio / Q000737:chemistry*
D005231:Fatty Acids, Unsaturated