title: Smooth muscle differentiation in human myometrium and uterine leiomyoma.
abstract: Smooth muscle differentiation has been analysed in human myometrium and leiomyoma by Western blotting with antibodies to smooth muscle specific proteins. No differences in the expression of h-caldesmon, metavinculin, desmin, alpha-smooth muscle actin and calponin were observed. The technique of two-dimensional gel electrophoresis was used, therefore, to further analyse differences between normal smooth muscle cells and their neoplastic counterparts. By comparing the protein patterns of normal myometrium and leiomyoma, it was possible to identify a protein with a molecular weight of approximately 27 kD that is selectively expressed in normal uterine smooth muscle cells. This protein proved to be a low molecular weight variant of calponin, a smooth muscle specific protein of as yet unknown function. Its immediate downregulation in tissue culture of normal myometrium points to a possible role in the process of dedifferentiation.
authors.affiliation: Institut für Molekularbiologie, Osterreichische Akademie der Wissenschaften, Salzburg, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Draeger
authors.forename: A H
authors.initials: AH
authors.lastname: Graf
authors.forename: A
authors.initials: A
authors.lastname: Staudach
authors.forename: A J
authors.initials: AJ
authors.lastname: North
authors.forename: J V
authors.initials: JV
authors.lastname: Small
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000737:chemistry; D002454:Cell Differentiation / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000737:chemistry / Q000473:pathology*; D008875:Middle Aged; D009124:Muscle Proteins / Q000032:analysis; D009130:Muscle, Smooth / Q000737:chemistry / Q000166:cytology*; D009215:Myometrium / Q000737:chemistry / Q000166:cytology; D014594:Uterine Neoplasms / Q000737:chemistry / Q000473:pathology*
chemical_list: D015415:Biomarkers; D009124:Muscle Proteins