FVT-1, a novel human transcription unit affected by variant translocation t(2;18)(p11;q21) of follicular lymphoma.
Variant t(2;18) and t(18;22) chromosome translocations observed in B-cell chronic lymphocytic leukemias and in follicular lymphomas have been reported to consistently involve the 5' region of the BCL-2 gene on chromosome 18 and various regions on the lg light chain loci. We show here that a variant t(2;18)(p11;q21) translocation observed in a case of follicular lymphoma leads to the juxtaposition of a J kappa segment to a chromosome 18 transcriptional unit located 10 kpb upstream of the BCL-2 locus. The cDNA of this new evolutionarily conserved gene, termed FVT-1 for follicular-variant-translocation gene, codes for a putatively secreted protein of 36 Kd that is not homologous with any described protein. The FVT-1 gene is weakly expressed in all the analyzed normal hematopoietic tissues but a very high rate of transcription is observed in some T-cell malignancies and in phytohemagglutinin-stimulated lymphocytes. The proximity of FVT-1 to the BCL-2 locus suggests that in the t(14;18) currently observed in follicular lymphomas, both genes would participate in the tumoral process.
Rimokh
R
R
Equipe de Cytologie Analytique et de Cytogénétique Moléculaire, Hopital Edouard Herriot, Lyon, France.
Gadoux
M
M
Berthéas
M F
MF
Berger
F
F
Garoscio
M
M
Deléage
G
G
Germain
D
D
Magaud
J P
JP
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002887:Chromosomes, Human, Pair 18; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis / Q000737:chemistry; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000737:chemistry / Q000235:genetics; D008224:Lymphoma, Follicular / Q000235:genetics*; D008969:Molecular Sequence Data; D012334:RNA, Neoplasm / Q000032:analysis; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D007145:Immunoglobulin kappa-Chains; D012334:RNA, Neoplasm; D004247:DNA