Interleukin 1 receptor on fibroblasts from systemic sclerosis patients induces excessive functional responses to interleukin 1 beta.
To determine if SSc fibroblasts are more sensitive to exogenous IL-1 than are normal fibroblasts, we studied the inductions of IL-6, prostaglandin E2 (PGE2), IL-1 beta mRNA production by IL-1-stimulated SSc and normal fibroblasts. The minimal IL-1 beta concentrations for stimulation of IL-6 and PGE2 production by SSc fibroblasts were 10-fold and 100-fold lower, respectively, than those for normal fibroblasts. The minimal IL-1 beta concentration for stimulation of IL-1 beta mRNA expression by SSc fibroblasts was also 10-fold lower than that for normal fibroblasts. These results suggest that the IL-1 signal transduction through IL-1R could be induced excessively in SSc fibroblasts. It is suggested that the IL-1 signal transduction plays an important role in the abnormal cytokine networks observed in the fibrosis of SSc.
Kawaguchi
Y
Y
First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Harigai
M
M
Suzuki
K
K
Hara
M
M
Kobayashi
K
K
Ishizuka
T
T
Matsuki
Y
Y
Tanaka
N
N
Nakamura
H
H
D001483:Base Sequence; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D017472:Receptors, Interleukin-1 / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values; D012595:Scleroderma, Systemic / Q000276:immunology*; D012867:Skin / Q000276:immunology*
D007375:Interleukin-1; D015850:Interleukin-6; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D015232:Dinoprostone