Immunohistochemical localization of gamma-aminobutyric acid- and aspartate-containing neurons in the guinea pig superior olivary complex.
The immunohistochemical localization of gamma-aminobutyric acid (GABA)- and aspartate-containing neurons was demonstrated in the guinea pig superior olivary complex, using purified antisera to GABA and aspartate, respectively. Medium-sized oval GABA-containing neurons were found in the lateral superior olive, and bipolar medium-sized GABA-containing neurons were observed in the dorsal hilus of the lateral superior olive. Medium-sized to large round GABA-containing neurons were observed in the ventral nucleus of the trapezoid body. GABA-containing terminals were found throughout the superior olivary complex with the highest density in the ventral nucleus of the trapezoid body. Aspartate-like immunoreactivity was observed in medium-sized round or oval neurons in the lateral superior olive, small fusiform neurons in the ventral nucleus of the trapezoid body, fusiform medium-sized neurons in the medial superior olive and oval medium-sized neurons in the superior paraolivary nucleus and round medium-sized neurons in the medial nucleus of the trapezoid body. Double staining method demonstrated that aspartate-containing neurons in the medial nucleus of the trapezoid body were surrounded by GABA-containing terminals. The present results suggested the possible origin of GABAergic and aspartatergic olivocochlear bundles.
Kumoi
K
K
Department of Otorhinolaryngology, Kobe University School of Medicine, Japan.
Saito
N
N
Tanaka
C
C
D000818:Animals; D001224:Aspartic Acid / Q000032:analysis*; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000737:chemistry*; D009847:Olivary Nucleus / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid