Simulated quantitative and qualitative isotachophoretic indices of 73 amino acids and peptides in the pH range 6.4-10.
Qualitative and quantitative isotachophoretic indices of 73 amino acids, dipeptides and tripeptides were simulated under 24 leading electrolyte conditions covering the pH range 6.4-10. The RE values and time-based zone lengths are tabulated together with the absolute mobility (m0) and pKa values used. The leading electrolyte used was 10 mM HCl and the pH buffers were imidazole, tris(hydroxymethylamino)methane, 2-amino-2-methyl-1,3-propanediol and ethanolamine. The simulated indices will be useful in the assessment of the separability and determination of the listed and related compounds.
Hirokawa
T
T
Faculty of Engineering, Hiroshima University, Japan.
Kiso
Y
Y
Gas
B
B
Zusková
I
I
Vacík
J
J
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D002021:Buffers; D004573:Electrolytes; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010455:Peptides / Q000737:chemistry*
D000596:Amino Acids; D002021:Buffers; D004573:Electrolytes; D010455:Peptides