Inhibition of neuritogenic ganglioside biosynthesis in chick brain neurons by calcium ionophore A23187.
Treatment with Ca++ ionophore A23187 blocks biosynthesis of the major terminally (IV3) sialylated higher species of neuronal gangliosides: GD1A, GT1B, GQ1B, and Gp, key components in the plasma membrane of neurite-bearing neurons. At the same time, the neurital field diminishes markedly, resulting in neuronal reversion to pre-neuritogenic morphology. A23187 causes no biosynthetic loss of any of the simpler gangliosides that constitute the plasma membrane of pre-neuritogenic neurons, nor of ganglioside precursors leading to formation of the blocked gangliosides. The data indicate that A23187 significantly reduces neuritogenic ganglioside biosynthesis.
Rosenberg
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Neuropsychiatric Institute, University of California, Los Angeles 90024-1759.
Noble
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EP
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology*; D000001:Calcimycin / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D005690:Galactose / Q000378:metabolism; D005732:Gangliosides / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism
D005732:Gangliosides; D012794:Sialic Acids; D000001:Calcimycin; D019158:N-Acetylneuraminic Acid; D005690:Galactose