title: Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB.
abstract: A system that detects the formation of complexes between different proteins by linking them to separate domains of the GAL4 transcription activator protein has been used to study protein-protein interactions between four essential and unique subunits of yeast RNA polymerase III (C82, C53, C34 and C31), the 70-kDa component of the initiation transcription factor IIIB (TFIIIB70) and the TATA-binding protein. We found that C82, C34, and C31 are able to combine with each other in vivo and that C34 interacts with TFIIIB70. These results suggest that C34 and TFIIIB70 are specificity determinants of the RNA polymerase III-TFIIIB interaction.
authors.affiliation: Département de Biologie Cellulaire et Moléculaire, Bâtiment 142, Centre d'Etudes de Saclay, Gif-sur-Yvette, France.
authors.forename: M
authors.initials: M
authors.lastname: Werner
authors.forename: N
authors.initials: N
authors.lastname: Chaussivert
authors.forename: I M
authors.initials: IM
authors.lastname: Willis
authors.forename: A
authors.initials: A
authors.lastname: Sentenac
mesh_terms: D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D012320:RNA Polymerase III / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016385:TATA Box; D035181:TATA-Box Binding Protein; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors; D012320:RNA Polymerase III