Stability of liposomes in vitro and their uptake by rat Peyer's patches following oral administration.
To evaluate the usefulness of liposomes as a carrier for the targeted delivery of antigens to gut-associated lymphoid tissue, liposomal stability and uptake by rat Peyer's patches were investigated. Liposomes composed of distearoylphosphatidylcholine, phosphatidylserine, and cholesterol (DSPC-liposome), or dipalmitoylphosphatidylcholine, phosphatidylserine, and cholesterol were stable in acidic solution (pH 2.0), diluted bile, and pancreatin solution. Following the oral administration of liposomes to rats, rhodamine B-PE incorporated in the lipid phase of DSPC-liposomes was preferentially taken up by Peyer's patches in the lower ileum. The uptake of rhodamine B-PE from DSPC-liposomes larger than 374 nm in mean diameter was high. Orally administered DSPC-liposomes of a large diameter thus appear to serve effectively as a vehicle for delivering antigens to Peyer's patches.
Aramaki
Y
Y
Tokyo College of Pharmacy, Japan.
Tomizawa
H
H
Hara
T
T
Yachi
K
K
Kikuchi
H
H
Tsuchiya
S
S
D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D004337:Drug Carriers; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D010316:Particle Size; D010581:Peyer's Patches / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012235:Rhodamines / Q000378:metabolism
D000941:Antigens; D004337:Drug Carriers; D008055:Lipids; D008081:Liposomes; D010713:Phosphatidylcholines; D012235:Rhodamines; C029773:rhodamine B