[Analysis of proteins and antigens in nine strains of Toxoplasma gondii by means of SDS-PAGE and immunoblotting].
The proteins and antigens of nine strains of Toxoplasma gondii were analyzed by SDS-PAGE and immunoblotting. These strains including RH strain, Zs-2 strain, CN strain and SH1, SH2, SH3, SH4, SH5, SH8 strains, were obtained from other laboratories or isolated from deformity fetus in our laboratory. SDS-PAGE analysis revealed that these strains were very close in the major bands of proteins and differences were observed only in part. There were common components in antigens of all strains using IgG antibody prepared from high-titre rabbit antisera raised against RH strain of Toxoplasma.
Jiang
C B
CB
Department of Parasitology, Shanghai Second Medical University.
Yang
H Z
HZ
Yang
H P
HP
Yang
Y
Y
Xu
K J
KJ
D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015800:Protozoan Proteins / Q000737:chemistry*; D013045:Species Specificity; D014122:Toxoplasma / Q000737:chemistry* / Q000276:immunology
D000953:Antigens, Protozoan; D015800:Protozoan Proteins