title: Proton magnetic resonance spectroscopy of vitreal changes in experimental streptococcal endophthalmitis.
abstract: We used magnetic resonance spectroscopy to examine endophthalmitis in rabbits inoculated with a virulent strain of Streptococcus pneumoniae. On different days after infection, the animals were sacrificed and the vitreous isolated and examined with water-suppressed proton magnetic resonance spectroscopy. A broad resonance corresponding to the methyl envelope of lipoprotein lipids appeared 2 days after infection and persisted until the eyes developed phthisis (around 10 days postinfection). This resonance was absent in the control eye and the bacterial culture; it could be used as the marker of breakdown of blood-vitreous barrier and onset of endophthalmitis-induced changes.
authors.affiliation: Howe Laboratory of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
authors.forename: H M
authors.initials: HM
authors.lastname: Cheng
authors.forename: M S
authors.initials: MS
authors.lastname: Hughes
authors.forename: K
authors.initials: K
authors.lastname: Lashkari
authors.forename: D
authors.initials: D
authors.lastname: Sang
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: J W
authors.initials: JW
authors.lastname: McMeel
authors.forename: A S
authors.initials: AS
authors.lastname: Baker
mesh_terms: D000818:Animals; D015415:Biomarkers; D004195:Disease Models, Animal; D009877:Endophthalmitis / Q000378:metabolism* / Q000382:microbiology; D015818:Eye Infections, Bacterial / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011008:Pneumococcal Infections / Q000378:metabolism*; D011817:Rabbits; D014822:Vitreous Body / Q000378:metabolism*
chemical_list: D015415:Biomarkers