title: Glycogen-bound protein in lower eukaryote and prokaryote.
abstract: The proteoglycogen fraction of Neurospora crassa was purified and subjected to radioiodination with [125I]iodide. Amylolysis of the polysaccharide moiety led to the isolation of a labelled 31 kDa-protein. The NH2-terminal amino acid sequence of 10 residues of the 31 kDa-protein was determined. A 31 kDa-protein was also bound to glycogen in Escherichia coli. Proteoglycogen has not been heretofore found in any primitive unicellular organism.
authors.affiliation: Departamento de Quimica Biologica-CIQUIBIC, Facultad de Ciencias Quimicas-CONICET Universidad Nacional de Cordoba, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Goldraij
authors.forename: M C
authors.initials: MC
authors.lastname: Miozzo
authors.forename: J A
authors.initials: JA
authors.lastname: Curtino
mesh_terms: D053674:Aggrecans; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002645:Chickens / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry*; D016326:Extracellular Matrix Proteins; D005656:Fungal Proteins / Q000032:analysis; D005964:Glucosyltransferases; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000032:analysis; D009492:Neurospora crassa / Q000737:chemistry*; D011509:Proteoglycans / Q000032:analysis*; D011817:Rabbits / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D053674:Aggrecans; D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D005656:Fungal Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D009124:Muscle Proteins; D011509:Proteoglycans; C051887:glycogenin; D005964:Glucosyltransferases