Evidence that murine V gamma 5 and V gamma 6 gamma delta-TCR+ lymphocytes are derived from a common distinct lineage.
Murine V gamma 5 and V gamma 6+ T lymphocytes develop sequentially in the thymus during fetal and newborn life, giving rise to intraepithelial lymphocytes of the epidermis and female reproductive tract. In analyzing a panel of gamma delta T cell hybridomas derived from various tissues, we found that all V gamma 6+ cells tested (n = 25) expressed V gamma 5-C gamma 1 mRNA, whereas none of a panel of V gamma 6- cells analyzed (n = 33) expressed this mRNA. V gamma 6 mRNA was rare in V gamma 5+ cells (1 of 9), and absent in all other gamma delta T cells (n = 10). These findings suggest that most, if not all, V gamma 6-TCR+ lymphocytes arise from precursor cells that have previously rearranged the V gamma 5 gene, and indicate that V gamma 5 and V gamma 6+ lymphocytes belong to a common, distinct lineage that does not give rise to other gamma delta T cells.
Heyborne
K
K
Department of Obstetrics and Gynecology, University of Colorado Health Sciences Center, Denver 80262.
Fu
Y X
YX
Kalataradi
H
H
Reardon
C
C
Roark
C
C
Eyster
C
C
Vollmer
M
M
Born
W
W
O'Brien
R
R
D000818:Animals; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006825:Hybridomas; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis* / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology*
D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta