title: Addition of a class IIS enzyme site in the mutagenic primer to improve two-step PCR-based targeted mutagenesis.
authors.affiliation: Laboratoire de Biochimie Microbienne/DBMS (CNRS URA 1130 alliée à l'INSERM), Centre d'Etudes Nucléaires de Grenoble, France.
authors.forename: J
authors.initials: J
authors.lastname: Armengaud
authors.forename: Y
authors.initials: Y
authors.lastname: Jouanneau
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005288:Ferredoxins / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D016354:Rhodobacter capsulatus / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D005288:Ferredoxins; C057073:endodeoxyribonuclease Ksp632I; D015252:Deoxyribonucleases, Type II Site-Specific