The effect of solvent/detergent-treated plasma on stored platelet concentrates.
The treatment of fresh-frozen plasma (FFP) with a solvent/detergent (S/D) solution to inactivate contaminating viruses has been shown to be effective in reducing virus transmission while maintaining the hemostatic properties of the plasma. FFP is treated with tri(n-butyl)phosphate (solvent) and Triton X-100 (detergent) and then purified; the in vivo effect of the residual S/D has been reported to be minimal. In clinical transfusion practice, ABO-incompatible, HLA-matched, single-donor platelets may have to be resuspended in ABO-compatible plasma. The use of S/D-treated plasma for this purpose would remove the added risk of transfusion-transmitted diseases due to the use of another blood component. As there are no data on the use of S/D-treated plasma as a platelet-resuspending medium, the potential toxicity of the residual solvent and detergent on the in vitro function and integrity of platelets stored in S/D-treated plasma for 5 days was studied. A repeated-measures analysis of variance was used for statistical analysis. Results showed that, as compared to controls (non-S/D-treated plasma), platelets resuspended in S/D-treated plasma maintained their functional properties, including morphology score and osmotic recovery, for up to 5 days of storage (p > 0.05, NS). No significant changes were seen among S/D-treated plasma and control groups for platelet count, lactate dehydrogenase discharge, beta-thromboglobulin release, glucose utilization, or generation of lactate. Measurement of pO2 and pCO2 values showed some differences between S/D-treated plasma and control groups that were significant, but not clinically significant. The pH values for all four groups ranged from 7.1 to 7.4 on Day 5.(ABSTRACT TRUNCATED AT 250 WORDS)
Tocci
L J
LJ
American Red Cross Blood Services, Connecticut Region, Farmington.
Napychank
P A
PA
Cable
R G
RG
Snyder
E L
EL
D000704:Analysis of Variance; D001786:Blood Glucose / Q000032:analysis; D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000592:standards; D003902:Detergents / Q000494:pharmacology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D010949:Plasma / Q000187:drug effects*; D010976:Platelet Count; D012997:Solvents / Q000494:pharmacology*
D001786:Blood Glucose; D003902:Detergents; D007773:Lactates; D012997:Solvents; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase