Stability of immunoadsorbents comprising antibody fragments. Comparison of Fv fragments and single-chain Fv fragments.
Immunoadsorbents comprising Fv fragments specific for hen egg lysozyme were used to recover the enzyme from a 20-fold excess of bovine albumin. We designed automatic equipment to run this model purification system for 100 cycles non-stop and monitored the deterioration of the immunoadsorbents during the cycling procedure. Only minor losses (approximately 25%) in the immunoadsorbents' capacity were detected; this correlated well with ligand loss (measured by enzyme-linked immunosorbent assay) which was approximately 0.2% per cycle. A surprising finding was that the use of "single-chain" Fv fragments conferred only a minor advantage with respect to stability of the immunoadsorbents.
Berry
M J
MJ
Immunology Department, Unilever Research, Sharnbrook, Bedfordshire, UK.
Pierce
J J
JJ
D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity / Q000295:instrumentation*; D004529:Egg White; D005069:Evaluation Studies as Topic; D007135:Immunoglobulin Variable Region / Q000737:chemistry*; D007164:Immunosorbents; D008024:Ligands; D009113:Muramidase / Q000276:immunology / Q000302:isolation & purification
D007135:Immunoglobulin Variable Region; D007164:Immunosorbents; D008024:Ligands; D009113:Muramidase