Rapid isolation of genes from bacterial lambda libraries by direct polymerase chain reaction screening.
A method for the direct screening of bacterial lambda libraries by polymerase chain reaction technology has been developed. This technique permits the identification and isolation of specific DNA sequences without the need for any filter hybridisation or radioactive probing. This strategy has been used to isolate a gene encoding lactate dehydrogenase from a Lactococcus lactis lambda library.
Griffin
H G
HG
Genetics and Microbiology Department, AFRC Institute of Food Research, Colney, Norwich, UK.
I'Anson
K J
KJ
Gasson
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D015723:Gene Library; D005798:Genes, Bacterial; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D007770:L-Lactate Dehydrogenase