Multimycotoxin detection and clean-up method for aflatoxins, ochratoxin and zearalenone in animal feed ingredients using high-performance liquid chromatography and gel permeation chromatography.
A sensitive and reliable method is described for the determination of aflatoxins B1, B2, G1 and G2, ochratoxin A and zearalenone in animal feed ingredients. A multi-toxin extraction and clean-up procedure is used, with dichloromethane-1 M hydrochloric acid (10:1) being used for the extraction and gel permeation chromatography being used for the clean-up. The liquid chromatographic method developed for the separation of the six mycotoxins involves gradient elution with a reversed-phase C18 column and fluorescence detection. Recoveries, repeatability and reproducibility have been determined on maize, palm and wheat. The detection limits varied depending on the type of feed.
Dunne
C
C
School of Chemical Sciences, Dublin City University, Glasnevin, Ireland.
Meaney
M
M
Smyth
M
M
Tuinstra
L G
LG
D000348:Aflatoxins / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008752:Methylene Chloride; D009793:Ochratoxins / Q000032:analysis*; D010944:Plants / Q000737:chemistry; D015203:Reproducibility of Results; D014908:Triticum / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis*
D000348:Aflatoxins; D009793:Ochratoxins; D008752:Methylene Chloride; D015025:Zearalenone