title: Introduction of five potentially metabolizable linking groups between 111In-cyclohexyl EDTA derivatives and F(ab')2 fragments of anti-carcinoembryonic antigen antibody--I. A new reproducible synthetic method.
abstract: The purpose of this study was to synthesize new bifunctional linker-chelating agents for the modification of the in vivo distribution of 111In-labeled antibodies. A general simple synthetic method of preparing cyclohexyl EDTA (CDTA) derivatives containing a linker/spacer group is described. Linkers prepared included a diester, a six carbon aliphatic chain, two thioethers and a disulfide group. The CDTA-linker compounds were coupled to F(Ab')2 fragments of anti-carcinoembryonic antigen monoclonal antibody and labeled with 111In with good retention of immunoreactivity.
authors.affiliation: INSERM Unit 211, Nantes, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Gestin
authors.forename: A
authors.initials: A
authors.lastname: Faivre-Chauvet
authors.forename: R C
authors.initials: RC
authors.lastname: Mease
authors.forename: C
authors.initials: C
authors.lastname: Sai-Maurel
authors.forename: P
authors.initials: P
authors.lastname: Thédrez
authors.forename: M
authors.initials: M
authors.lastname: Slinkin
authors.forename: G E
authors.initials: GE
authors.lastname: Meinken
authors.forename: S C
authors.initials: SC
authors.lastname: Srivastava
authors.forename: J F
authors.initials: JF
authors.lastname: Chatal
mesh_terms: D000818:Animals; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D002614:Chelating Agents / Q000138:chemical synthesis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000378:metabolism; D004355:Drug Stability; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D007128:Immunoglobulin Fragments / Q000737:chemistry* / Q000378:metabolism; D007205:Indium Radioisotopes / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods; D051379:Mice; D015203:Reproducibility of Results
chemical_list: D002272:Carcinoembryonic Antigen; D002614:Chelating Agents; D003432:Cross-Linking Reagents; D007128:Immunoglobulin Fragments; D007205:Indium Radioisotopes; C005390:CDTA; D004492:Edetic Acid