New approach for atomic force microscopy of membrane proteins. The imaging of cholera toxin.
We demonstrate that supported synthetic phospholipid bilayers, which are stabilized by lateral cross-linking in both leaflets, can be used for specimen preparation for atomic force microscopy of purified membrane proteins with high stability and excellent reproducibility under water or low-salt buffer. A bilayer containing 1,2-dipentacosa-10,12-diynoyl-phosphatidylcholine and 20 mol % ganglioside (GM1) was transferred onto the surface of mica from a Langmuir trough. Cholera toxin, both the B-subunit and the complete molecular randomly bound to the gangliosides, were imaged by atomic force microscopy in solution with a resolution of better than 2 nm. The pentameric structure of the B-subunit oligomers was well resolved. This result indicates that, with this preparation procedure, other membrane proteins may be studied at intermediate to high resolution under physiologically relevant conditions without the need for crystallization.
Yang
J
J
Bio-SPM Laboratory, University of Virginia, Charlottesville 22908.
Tamm
L K
LK
Tillack
T W
TW
Shao
Z
Z
D002772:Cholera Toxin / Q000737:chemistry*; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000648:ultrastructure*; D008853:Microscopy / Q000379:methods*
D008051:Lipid Bilayers; D008565:Membrane Proteins; D002772:Cholera Toxin