title: Investigation of the human metabolism of antipyrine using coupled liquid chromatography and nuclear magnetic resonance spectroscopy of urine.
abstract: The potential of coupled high-performance liquid chromatography-nuclear magnetic resonance spectroscopy for the detection and identification of drug metabolites has been investigated by direct analysis of human urine collected following administration of antipyrine. This approach provided a rapid method of characterizing the major human urinary metabolites of this drug and promises to be of widespread value in structural studies of xenobiotic metabolites.
authors.affiliation: Department of Safety of Medicines, Zeneca Pharmaceuticals, Maccelesfield, Cheshire, UK.
authors.forename: I D
authors.initials: ID
authors.lastname: Wilson
authors.forename: J K
authors.initials: JK
authors.lastname: Nicholson
authors.forename: M
authors.initials: M
authors.lastname: Hofmann
authors.forename: M
authors.initials: M
authors.lastname: Spraul
authors.forename: J C
authors.initials: JC
authors.lastname: Lindon
mesh_terms: D000983:Antipyrine / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000983:Antipyrine