Screening for TP53 mutations in osteosarcomas using constant denaturant gel electrophoresis (CDGE).
We have previously developed conditions to screen for TP53 point mutations inside the conserved domains II-V of the gene by using constant denaturant gel electrophoresis (CDGE). The present study reports conditions for screening more of the codons in the frequently mutated region exon 5-8 and for detecting mutations in sequences encoding functional domains in the N- and C-terminal part of the protein. The ability of the CDGE technique to detect mutations was studied using controls with known sequence deviations. The resolution power of the technique to separate different types of mutations was tested by using seven different single base pair mutants all residing in a stretch of four base pairs. All mutants were separated from the wild type. The established CDGE screening strategy was then used to look for mutations in DNA from 28 osteosarcomas. Six (21.5%) of the samples were shown to have a TP53 mutation, and the exact characterization was performed by direct sequencing. All of these were within the frequently reported mutated region exon 5-8.
Smith-Sørensen
B
B
Department of Genetics, Norwegian Radium Hospital, Montebello, Oslo.
Gebhardt
M C
MC
Kloen
P
P
McIntyre
J
J
Aguilar
F
F
Cerutti
P
P
Børresen
A L
AL
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics*; D002648:Child; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers; D004586:Electrophoresis / Q000379:methods*; D005260:Female; D005820:Genetic Testing; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D012516:Osteosarcoma / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D017931:DNA Primers; D016159:Tumor Suppressor Protein p53