title: Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys.
abstract: Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.
authors.affiliation: Immunopathology Laboratory, Karolinska Institute, Stockholm, Sweden.
authors.forename: S L
authors.initials: SL
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Feichtinger
authors.forename: E
authors.initials: E
authors.lastname: Kaaya
authors.forename: P
authors.initials: P
authors.lastname: Migliorini
authors.forename: P
authors.initials: P
authors.lastname: Putkonen
authors.forename: G
authors.initials: G
authors.lastname: Biberfeld
authors.forename: J M
authors.initials: JM
authors.lastname: Middeldorp
authors.forename: P
authors.initials: P
authors.lastname: Biberfeld
authors.forename: I
authors.initials: I
authors.lastname: Ernberg
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D034961:Antigens, Nuclear; D000956:Antigens, Viral / Q000378:metabolism*; D015153:Blotting, Western; D003429:Cross Reactions; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000276:immunology*; D008252:Macaca fascicularis; D009687:Nuclear Proteins / Q000032:analysis; D015302:Simian Immunodeficiency Virus / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D034961:Antigens, Nuclear; D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D009687:Nuclear Proteins