title: Isolation of a retrovirus from two fish cell lines developed from chinook salmon (Oncorhynchus tshawytscha) with plasmacytoid leukaemia.
abstract: Two new cell lines developed from chinook salmon with plasmacytoid leukaemia have been found to be producing a virus. The virus has been identified as a retrovirus based on: type of c.p.e. induced in culture; morphology and density of the particle; presence of Mn(2+)-dependent, poly(rA)-directed reverse transcriptase activity which was associated with a density of 1.16 to 1.18 g/ml in sucrose; electrophoretic pattern of the polypeptides from purified virions; elevated [3H]UTP labelling of RNA in the cell cultures occurring at a density of 1.16 to 1.18 g/ml in sucrose. This report describes the first isolation of a retrovirus from a salmonid cell line.
authors.affiliation: Malaspina University College, Nanaimo, British Columbia, Canada.
authors.forename: W D
authors.initials: WD
authors.lastname: Eaton
authors.forename: B
authors.initials: B
authors.lastname: Folkins
authors.forename: J
authors.initials: J
authors.lastname: Bagshaw
authors.forename: G
authors.initials: G
authors.lastname: Traxler
authors.forename: M L
authors.initials: ML
authors.lastname: Kent
mesh_terms: D000818:Animals; D002460:Cell Line; D005393:Fish Diseases / Q000382:microbiology*; D007952:Leukemia, Plasma Cell / Q000382:microbiology / Q000662:veterinary*; D012190:Retroviridae / Q000302:isolation & purification*; D012474:Salmon / Q000382:microbiology*