The target of ammonia action in dictyostelium.
The weak base ammonia is implicated in a number of key processes in Dictyostelium development, notably aggregation and culmination. To determine its intracellular site of action, we compared its biological potency with that of other weak bases. All the bases inhibited these developmental processes effectively, but differed manyfold in potency in accordance with their activity in dissipating pH gradients, as measured by in vivo 31P NMR with pH-sensitive phosphonate probes. These results indicate that weak bases influence development by raising the pH of an intracellular acidic compartment.
Davies
L
L
Department of Biochemistry, University of Oxford, England.
Satre
M
M
Martin
J B
JB
Gross
J D
JD
D000468:Alkalies / Q000494:pharmacology*; D000588:Amines / Q000494:pharmacology; D000641:Ammonia / Q000494:pharmacology*; D000818:Animals; D002451:Cell Compartmentation; D002738:Chloroquine / Q000494:pharmacology; D003593:Cytoplasm / Q000502:physiology; D004023:Dictyostelium / Q000187:drug effects* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009024:Morphogenesis / Q000187:drug effects*; D014617:Vacuoles / Q000502:physiology
D000468:Alkalies; D000588:Amines; D000641:Ammonia; D002738:Chloroquine