Occurrence of 2-O-methyl-N-(3-deoxy-L-glycero-tetronyl)-D-perosamine (4-amino-4,6-dideoxy-D-manno-pyranose) in lipopolysaccharide from Ogawa but not from Inaba O forms of O1 Vibrio cholerae.
A structural study by GC-MS, methylation analysis, and 1H and 13C NMR was carried out on alpha (1-->2)-linked linear N-(3-deoxy-L-glycero-tetronyl)-D-perosamine homopolymer constituting the O-polysaccharide chain of lipopolysaccharide from O1 Vibrio cholerae Ogawa and Inaba O forms. Occurrence of 2-O-methyl-N-(3-deoxy-L-glycero-tetronyl)-D-perosamine was demonstrated at the non-reducing terminus of the perosamine-homopolymer of lipopolysaccharide from the Ogawa O form in contrast to the presence of N-(3-deoxy-L-glycero-tetronyl)-D-perosamine at the nonreducing terminus for the Inaba O form.
Hisatsune
K
K
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Kondo
S
S
Isshiki
Y
Y
Iguchi
T
T
Haishima
Y
Y
D002236:Carbohydrate Conformation; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000031:analogs & derivatives* / Q000032:analysis; D008745:Methylation; D009844:Oligosaccharides / Q000737:chemistry; D013045:Species Specificity; D014734:Vibrio cholerae / Q000737:chemistry* / Q000145:classification
D008070:Lipopolysaccharides; D009844:Oligosaccharides; C057033:4-amino-4,6-dideoxy-D-mannose; D008358:Mannose