title: Expression, purification and crystallization of fully active, glycosylated human interleukin-5.
abstract: Recombinant human interleukin-5 (hIL-5) has been expressed at high levels and produced in large quantities in baculovirus infected Sf9 insect cells. The glycosylated protein was purified using immuno-affinity chromatography and gel filtration. Purified hIL-5 has been crystallized using standard vapour diffusion techniques with PEG as a coprecipitant. The crystals belong to the C2 space group and diffract to 2 A.
authors.affiliation: Roche Research Gent, Belgium.
authors.forename: Y
authors.initials: Y
authors.lastname: Guisez
authors.forename: C
authors.initials: C
authors.lastname: Oefner
authors.forename: F K
authors.initials: FK
authors.lastname: Winkler
authors.forename: E J
authors.initials: EJ
authors.lastname: Schlaeger
authors.forename: M
authors.initials: M
authors.lastname: Zulauf
authors.forename: J
authors.initials: J
authors.lastname: Van der Heyden
authors.forename: G
authors.initials: G
authors.lastname: Plaetinck
authors.forename: S
authors.initials: S
authors.lastname: Cornelis
authors.forename: J
authors.initials: J
authors.lastname: Tavernier
authors.forename: W
authors.initials: W
authors.lastname: Fiers
mesh_terms: D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D003001:Cloning, Molecular; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D015848:Interleukin-5 / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008027:Light; D051379:Mice; D008819:Mice, Nude; D009036:Moths; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012542:Scattering, Radiation
chemical_list: D015848:Interleukin-5; D011994:Recombinant Proteins