title: Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia.
abstract: Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the beta-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric beta-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.
authors.affiliation: Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados (CINVESTAV), Guanajuato, Mexico.
authors.forename: A M
authors.initials: AM
authors.lastname: Bailey
authors.forename: G L
authors.initials: GL
authors.lastname: Mena
authors.forename: L
authors.initials: L
authors.lastname: Herrera-Estrella
mesh_terms: D015139:Blotting, Southern; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers; D005821:Genetic Techniques; D005966:Glucuronidase / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology; D010838:Phytophthora / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D010957:Plasmids; D014170:Transformation, Genetic; D014414:Tungsten
chemical_list: D004274:DNA, Recombinant; D005819:Genetic Markers; D006921:Hygromycin B; D005966:Glucuronidase; D014414:Tungsten