Change in location and processing of inhibin alpha-subunit precursors during sexual maturation of the Djungarian hamster testis.
Immunohistochemical location and immunoblot of inhibin alpha-subunit peptides were analyzed in the testis of the Djungarian hamster from days 0-31 of postnatal development using a specific antibody. An intense immunoreaction was observed in the centrally located T1 prespermatogonia at day 0. The staining intensity decreased gradually in the spermatogonia when they make contact with the basal lamina at days 8-10. At days 13 and 15 there is no staining. Thereafter the immunoreactivity in Sertoli cells as well as in A spermatogonia gradually increased, being highest in sexually mature animals. The intensity of alpha-subunit staining in the seminiferous tubules was stage specific, being strongest at stages III and IV. Immunoblot analysis of testis homogenates with the anti-INH alpha 1-32 antibody showed several bands: 88K, 80K, and 43K in immature hamster testis (0-, 2-, 6-, 8-, or 10-day-old). In the adult hamster (31-day-old) 88K, 80K, 28K, and 20K bands were seen, but no 43K band. Dimeric inhibin was not detected. The 43-44K band most likely corresponds to the pro-alpha N alpha C, the 28K band to intermediate forms between alpha N alpha C and alpha C (alpha I alpha C), and the 20K band to mature alpha-subunit (alpha C). The shift from the immature pattern to mature occurs at about 20 days of age. Freezing of the samples was deleterious to alpha C, since it could be detected only in freshly homogenized samples. The results suggest that prespermatogonia produce predominantly monomeric alpha-subunit precursor pro-alpha N alpha C, whereas the mature Sertoli cells as well as A spermatogonia contain mainly monomeric alpha I alpha C. The alpha-inhibin precursors may act as auto-/paracrine regulators of spermatogenesis. Our results suggest that different alpha-subunit precursors, pro-alpha N alpha C and alpha I alpha C, might be involved in the differentiation and maintenance of spermatogenesis, respectively. The posttranslational processing of alpha-subunit precursors seems to play an important role in the physiology of reproduction.
Tuohimaa
P
P
Department of Biomedical Sciences, University of Tampere, Finland.
Bläuer
M
M
Bergmann
M
M
Aumüller
G
G
D000375:Aging / Q000502:physiology*; D000818:Animals; D006224:Cricetinae; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007265:Inhibins / Q000032:analysis* / Q000096:biosynthesis; D008297:Male; D017111:Phodopus; D011498:Protein Precursors / Q000032:analysis* / Q000096:biosynthesis; D012671:Seminiferous Tubules / Q000502:physiology; D012741:Sexual Maturation / Q000502:physiology*; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000502:physiology*
D011498:Protein Precursors; D007265:Inhibins