[A technique for bone marrow biopsy specimen preservation].
The rapidly frozen bone marrow specimen was displaced in iced acetone at low temperature first and was embedded in low-temperature glycol methacrylate resin subsequently which was then ready for cryostat section preparation. Since there was no routine fixation required, so that the morphology features will be well preserved for further immunohistochemical and histochemical studies. By this technique, the enzyme activity and localization site will also be well preserved, particularly the lymphoid cell surface antigens, and it is also valuable for retrospective study of biopsy specimens.
Liu
S Q
SQ
Union Hospital, Tongji Medical University, Wuhan.
D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology*; D015925:Cryopreservation; D006801:Humans; D007150:Immunohistochemistry; D007938:Leukemia / Q000473:pathology; D009196:Myeloproliferative Disorders / Q000473:pathology; D016610:Tissue Embedding / Q000379:methods*