title: Isolation and characterization of an ornithine aminotransferase-related sequence (OATL3) mapping to 10q26.
abstract: We used a near full-length human ornithine delta-amino-transferase cDNA, huOAT6, as a probe under low stringency hybridization conditions to identify a new autosomal ornithine delta-aminotransferase-related sequence (OATL3). Cloning and characterization of this sequence reveal it to be a partial nonprocessed pseudogene corresponding to exon 3 and flanking intronic sequences of the ornithine delta-aminotransferase structural gene. Using somatic cell hybrids and fluorescence in situ hybridization, we mapped OATL3 to 10q26, adjacent to the ornithine delta-aminotransferase structural gene locus.
authors.affiliation: Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: M T
authors.initials: MT
authors.lastname: Geraghty
authors.forename: W G
authors.initials: WG
authors.lastname: Kearns
authors.forename: P L
authors.initials: PL
authors.lastname: Pearson
authors.forename: D
authors.initials: D
authors.lastname: Valle
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D005091:Exons; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007438:Introns; D007621:Karyotyping; D008677:Metaphase; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D009953:Ornithine-Oxo-Acid Transaminase