Identification and characterization of an anti-tyrosine kinase factor in cystic gliomas.
In view of the frequent activation of the epidermal growth factor receptor (EGF-R) in gliomas and autocrine hypothesis, we searched for 'EGF-like' factor(s) in cystic fluids (CFs) associated with gliomas. Membranes of A431 cells, which overexpress EGF-R, were used to explore such activity in 20 CFs. In all cases CFs induced inhibition of EGF-R phosphorylation. Biochemical analysis revealed an anti-tyrosine kinase activity which was identified as a 18 kDa proteic factor. Effectiveness at high dilution and anti-proliferative effect on living cells in culture suggest that this factor may be involved in the negative regulation of glial oncogenesis.
Berger
F
F
INSERM U. 318, CHU Grenoble, France.
Laine
M
M
Amalfitano
G
G
Brunet
J F
JF
Verna
J M
JM
Nissou
M F
MF
Benabid
A L
AL
D001254:Astrocytoma / Q000737:chemistry*; D001932:Brain Neoplasms / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured
D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases