Lysosome-associated membrane protein-1 (LAMP-1) is the melanocyte vesicular membrane glycoprotein band II.
Coated vesicles play a critical role in the process of melanogenesis. Antisera raised against a coated vesicle fraction from mouse melanoma cells recognize two major glycoprotein antigens, band I (47-55 kd) and band II (90-120 kd). We demonstrate that band II is lysosome-associated membrane protein 1 (LAMP-1) by the following criteria: 1) the molecular weight and abundance of LAMP-1 varies among tissues but is always identical to that of band II; 2) band II and LAMP-1 co-migrate in sucrose gradient sedimentation studies; 3) immunodepletion of cell extracts with antivesicle serum removes all LAMP-1; and 4) intact organelles immunoisolated with antivesicle serum contain band II and LAMP-1. Our results further confirm the long-suspected relationship between melanosomes and the lysosomal lineage of organelles.
Zhou
B K
BK
Ronald O. Perelman Department of Dermatology, New York University Medical Center, New York.
Boissy
R E
RE
Pifko-Hirst
S
S
Moran
D J
DJ
Orlow
S J
SJ
D000818:Animals; D015703:Antigens, CD; D002499:Centrifugation, Density Gradient; D005455:Fluorescent Antibody Technique; D051907:Lysosomal Membrane Proteins; D008544:Melanocytes / Q000737:chemistry*; D008546:Melanoma, Experimental / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D013395:Sucrose; D016608:Talin / Q000302:isolation & purification
D015703:Antigens, CD; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D016608:Talin; D013395:Sucrose