Structural analysis of the CD11b gene and phylogenetic analysis of the alpha-integrin gene family demonstrate remarkable conservation of genomic organization and suggest early diversification during evolution.
CD11b is a member of the beta 2 subfamily of the human leukocyte integrins. Its expression is limited to mature myeloid and NK cells and is up-regulated during the course of granulocytic and monocytic differentiation. The CD11b/CD18 (Mo1) heterodimer promotes adhesion of granulocytes and monocytes to C3bi-coated bacteria and endothelial cells. In an attempt to relate the exon structure to the known functional domains, as well as to identify and study cis-acting elements that are involved in its tissue-specific expression, we have isolated genomic clones encoding CD11b, deduced the exon/intron organization, and determined the transcriptional start site. The CD11b gene spans 55 kb and is encoded by 30 exons. Its structure closely resembles that of CD11c, another of the three leukocyte integrin alpha-chains, and suggests that these two genes arose by a gene duplication event. Furthermore, comparison of the CD11b gene structure with that of platelet glycoprotein IIb and Drosophila PS2 suggest how the human leukocyte integrins evolved and dispersed during the course of evolution.
Fleming
J C
JC
Hematology/Oncology Division, Beth Israel Hospital, Boston, MA 02215.
Pahl
H L
HL
Gonzalez
D A
DA
Smith
T F
TF
Tenen
D G
DG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D017124:Conserved Sequence; D004330:Drosophila / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D016023:Integrins / Q000235:genetics*; D016177:Macrophage-1 Antigen / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D016023:Integrins; D016177:Macrophage-1 Antigen