The effects of sevoflurane on intracellular Ca2+ regulation in rat hepatocytes.
The effects of sevoflurane, a new volatile anesthetic agent undergoing clinical trial, on the mobilization of intracellular Ca2+ in isolated rat hepatocytes was studied. This agent produced a dose-dependent release of 45Ca2+ from internal, non-mitochondrial stores of permeabilized hepatocytes (saponin treated). However, the administration of sevoflurane to aequorin-loaded intact hepatocytes had little or no effect on intracellular [Ca2+] (i.e., short transient or no increases in luminescence: no toxic effect). These data may indicate that because of the low solubility of sevoflurane, it has a selective effect on endoplasmic reticulum, i.e., mobilizing internal stores of Ca2+ relative to increasing transmembrane fluxes.
Iaizzo
P A
PA
Department of Anesthesiology, University of Minnesota, Minneapolis 55455.
Seewald
M J
MJ
Powis
G
G
Van Dyke
R A
RA
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D016923:Cell Death; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D004987:Ethers / Q000494:pharmacology*; D006706:Homeostasis; D007424:Intracellular Fluid / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008738:Methyl Ethers; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology; D000077149:Sevoflurane
D000777:Anesthetics; D004987:Ethers; D008738:Methyl Ethers; D012503:Saponins; D000077149:Sevoflurane; D002118:Calcium