title: Identification of a purC gene from Streptococcus pneumoniae.
abstract: A gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase was identified in Streptococcus pneumoniae as a 708-bp segment of the genome encoding a 27,001-Da protein with strong similarity to known PurC proteins. The S. pneumoniae purC gene, found immediately adjacent to the competence induction genes, comAB, was cloned and sequenced. The predicted protein product of purC displayed substantial (> 40%) identity to the entire sequence of the PurC proteins of Bacillus subtilis and Escherichia coli. Function of the S. pneumoniae gene product was demonstrated by complementation of E. coli purC mutations.
authors.affiliation: Department of Biological Sciences, University of Illinois at Chicago 60680.
authors.forename: F M
authors.initials: FM
authors.lastname: Hui
authors.forename: D A
authors.initials: DA
authors.lastname: Morrison
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D010453:Peptide Synthases; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; D010453:Peptide Synthases; C083513:PurC protein, Bacteria; C022379:phosphoribosylaminoimidazole-succinocarboxamide synthetase