Expression of highly active sex-inducing pheromone of Volvox carteri f. nagariensis in a mammalian cell system.
A cDNA fragment coding for the sex-inducing glycoprotein of Volvox carteri f. nagariensis was expressed in a mammalian cell system (baby hamster kidney (BHK) cells). The transfection product exhibited a specific biological activity intermediate between the natural pheromone of the strains Volvox carteri f. nagariensis and Volvox carteri f. weismannia. Immunoblot analysis showed that the sex-inducing activity was expressed as a set of three iso-glycoproteins (35, 34 and 31 kDa).
Jaenicke
L
L
Institute of Biochemistry, University of Cologne, Germany.
Feldwisch
O
O
Merkl
B
B
Cremer
A
A
Haas
I G
IG
D002478:Cells, Cultured; D000460:Chlorophyta / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D066298:In Vitro Techniques; D008970:Molecular Weight; D012724:Sex Attractants / Q000235:genetics*; D014162:Transfection
D006023:Glycoproteins; D012724:Sex Attractants; D004247:DNA