title: Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis.
abstract: In analyzing the region of the Saccharopolyspora erythraea chromosome responsible for the biosynthesis of the macrolide antibiotic erythromycin, we identified a gene, designated eryK, located about 50 kb downstream of the erythromycin resistance gene, ermE. eryK encodes a 44-kDa protein which, on the basis of comparative analysis, belongs to the P450 monooxygenase family. An S. erythraea strain disrupted in eryK no longer produced erythromycin A but accumulated the B and D forms of the antibiotic, indicating that eryK is responsible for the C-12 hydroxylation of the macrolactone ring, one of the last steps in erythromycin biosynthesis.
authors.affiliation: Corporate Molecular Biology, Abbott Laboratories, Abbott Park, Illinois 60064.
authors.forename: D
authors.initials: D
authors.lastname: Stassi
authors.forename: S
authors.initials: S
authors.lastname: Donadio
authors.forename: M J
authors.initials: MJ
authors.lastname: Staver
authors.forename: L
authors.initials: L
authors.lastname: Katz
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002874:Chromosome Mapping; D016384:Consensus Sequence; D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000235:genetics*; D004917:Erythromycin / Q000096:biosynthesis*; D005798:Genes, Bacterial / Q000235:genetics*; D006900:Hydroxylation; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010105:Oxygenases / Q000145:classification / Q000235:genetics*; D016930:Saccharopolyspora / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004917:Erythromycin; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C078882:EryK protein, Saccharopolyspora erythraea