Repression of stromelysin metalloprotease expression in rat fibrosarcoma cells by dimethylsulfoxide.
Metalloproteases are implicated in conferring invasive properties to tumor cells. We show here that treatment of ras-oncogene-transformed rat fibroblasts with dimethylsulfoxide (DMSO) results in a reversible decrease in stromelysin mRNA. Furthermore, stromelysin expression was found to be repressed by DMSO, but not by glucocorticoid hormone, in a fibrosarcoma cell line showing low AP-1 (fos/jun) transcription factor activity. In two fibrosarcoma cell lines which express high levels of stromelysin and low levels of 68 kDa type IV collagenase, the DMSO-induced decrease in stromelysin expression was paralleled by a decreased invasive propensity.
Popowicz
P
P
Department of Oncology, Radiumhemmet, Karolinska Institute and Hospital, Stockholm, Sweden.
Engel
G
G
Marshall
H
H
Linder
S
S
D000818:Animals; D015152:Blotting, Northern; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004794:Enzyme Repression / Q000187:drug effects; D005354:Fibrosarcoma / Q000201:enzymology* / Q000473:pathology; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000187:drug effects*; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D004121:Dimethyl Sulfoxide