title: Specific T-cell recognition of the merozoite proteins rhoptry-associated protein 1 and erythrocyte-binding antigen 1 of Plasmodium falciparum.
abstract: The merozoite proteins merozoite surface protein 1 (MSP-1) and rhoptry-associated protein 1 (RAP-1) and synthetic peptides containing sequences of MSP-1, RAP-1, and erythrocyte-binding antigen 1, induced in vitro proliferative responses of lymphocytes collected from Ghanaian blood donors living in an area with a high rate of transmission of malaria. Lymphocytes from a large proportion of the Ghanaian blood donors proliferated in response to the RAP-1 peptide, unlike those of Danish control blood donors, indicating that this sequence contains a malaria-specific T-cell epitope broadly recognized by individuals living in an area with a high transmission rate of malaria. Most of the donor plasma samples tested contained immunoglobulin G (IgG) and IgM antibodies recognizing the merozoite proteins, while only a minority showed high IgG reactivity to the synthetic peptides.
authors.affiliation: Department of Infectious Diseases, University Hospital, Copenhagen, Denmark.
authors.forename: P H
authors.initials: PH
authors.lastname: Jakobsen
authors.forename: L
authors.initials: L
authors.lastname: Hviid
authors.forename: T G
authors.initials: TG
authors.lastname: Theander
authors.forename: E A
authors.initials: EA
authors.lastname: Afare
authors.forename: R G
authors.initials: RG
authors.lastname: Ridley
authors.forename: P M
authors.initials: PM
authors.lastname: Heegaard
authors.forename: D
authors.initials: D
authors.lastname: Stuber
authors.forename: K
authors.initials: K
authors.lastname: Dalsgaard
authors.forename: F K
authors.initials: FK
authors.lastname: Nkrumah
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000953:Antigens, Protozoan; D000954:Antigens, Surface / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D016778:Malaria, Falciparum / Q000276:immunology*; D020066:Merozoite Surface Protein 1; D008875:Middle Aged; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology*; D011956:Receptors, Cell Surface; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D002352:Carrier Proteins; C066001:Duffy antigen binding protein, Plasmodium; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; C065352:rhoptry associated protein, Plasmodium