Infection of differentiated U937 cells by Salmonella typhimurium: absence of correlation between oxidative burst and antimicrobial defence.
The human histiocytic lymphoma cell line U937 can be induced to differentiate along the monocyte/macrophage pathway by either phorbol myristate acetate (PMA) or by the combination of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (VD). U937 cells treated with either PMA or RA/VD were able to phagocytose Salmonella typhimurium in the presence of non-immune human serum. However, only cells differentiated by RA/VD were capable of developing an oxidative metabolic burst in response to infection. Since the oxidative burst is considered to be a potent antimicrobial mechanism, we investigated its effect on S. typhimurium. The oxidative burst failed to affect either the viability or the multiplication of S. typhimurium suggesting that it plays only a minor role in the host defence against S. typhimurium.
Château
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MT
INSERM U65, Département de Biologie-Santé, Université de Montpellier II, France.
Caravano
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D002454:Cell Differentiation / Q000187:drug effects; D002762:Cholecalciferol / Q000494:pharmacology; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D010587:Phagocytosis; D016897:Respiratory Burst; D012486:Salmonella typhimurium / Q000254:growth & development / Q000276:immunology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D002762:Cholecalciferol; D014212:Tretinoin