Improved bacterial hosts for regulated expression of genes from lambda pL plasmid vectors.
The construction and use of a set of Escherichia coli strains with defective lambda prophages that facilitate expression of genes cloned in lambda pL-plasmid vectors is described. These bacteria allow high and regulated expression of such genes, whereas a kanamycin-resistance marker (KmR) on the prophage allows easy identification and genetic transfer from strain to strain. Optimal conditions for examining gene expression with the pL-vector systems using these strains are discussed.
Patterson
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ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, MD 21702-1201.
Costantino
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N
Dasgupta
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Court
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DL
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D003673:Defective Viruses / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids