Positive regulation of the Escherichia coli glycine cleavage enzyme system.
A new mutation in Escherichia coli, designated gcvA1, that results in noninducible expression of both gcv and a gcvT-lacZ gene fusion was isolated. A plasmid carrying the wild-type gcvA gene complemented the mutation and restored glycine-inducible gcv and gcvT-lacZ gene expression. These results suggest that gcvA encodes a positive-acting regulatory protein that acts in trans to increase expression of gcv.
Wilson
R L
RL
Department of Microbiology, University of Iowa, Iowa City 52242.
Steiert
P S
PS
Stauffer
G V
GV
D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005998:Glycine / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D014166:Transferases / Q000235:genetics / Q000378:metabolism*
D002352:Carrier Proteins; D009097:Multienzyme Complexes; C064525:glycine cleavage system; D000594:Amino Acid Oxidoreductases; D014166:Transferases; D005998:Glycine