Involvement of annexin II in DNA replication: evidence from cell-free extracts of Xenopus eggs.
Cell-free extracts of Xenopus eggs efficiently initiate and complete semiconservative DNA replication of exogenously added plasmid DNA. DNA replication in such extracts can be neutralized by a monoclonal antibody (D1/274.5) against human annexin II. Specific immunodepletion of Xenopus annexin II from the egg extracts results in loss of DNA replicative ability. Immunodepletion of annexin II does not prevent nuclear assembly, a stringent requirement for DNA synthesis on exogenous DNA in this system. Replicative ability can be restored to the immunodepleted extracts by the addition of purified human annexin II. These results demonstrate that annexin II is involved in chromosomal DNA replication and has a role in the cell cycle of higher eukaryotes.
Vishwanatha
J K
JK
Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha 68198-4525.
Kumble
S
S
D000818:Animals; D017306:Annexin A2 / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002474:Cell-Free System; D004261:DNA Replication / Q000187:drug effects / Q000502:physiology*; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D016253:Microscopy, Immunoelectron; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D010741:Phospholipases A / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D013045:Species Specificity; D014982:Xenopus laevis / Q000378:metabolism
D017306:Annexin A2; D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial; D010741:Phospholipases A