[NADPH-D reactive choroid ganglion cells in the human].
BACKGROUND
The demonstration of uveal ganglion cells has been investigated so far by silver impregnation, rather complicated neurohistochemical methods and by electron microscopy. We present the first results of a simple method in flat-preparation of the human choroid.


METHODS
Donors' eyes for cornea transplantation, 5 men, 3 women, aged 45 +/- 13 years, with postmortal time of preparation of 12 +/- 9 h (1 h-24 h) were used. After removal of the cornea with scleral ring, flat-preparations of the choroid were treated by the standard Nicotinamide-Adenine-Dinucleotide-Phosphate (NADPH)-diaphorase reaction, mounted on coated slides in Kayser's glycerol-gelatin and observed by light microscope.


RESULTS
NADPH-diaphorase reaction reveals abundant aggregates of ganglion cells, with nets of dendrites and axons in the otherwise unstained flat-preparation of the human choroidea. They are more abundant in the layer close to the sclera.


CONCLUSIONS
NADPH-diaphorase reaction is a simple method for the detection of ganglion cells in the human choroid. Their unexpectedly high number suggests yet unknown ganglional functions in the uvea.
Bergua
A
A
Augenklinik mit Poliklinik, Universität Erlangen-Nürnberg.
Jünemann
A
A
Naumann
G O
GO
D000328:Adult; D001369:Axons / Q000648:ultrastructure; D002829:Choroid / Q000033:anatomy & histology*; D005260:Female; D006652:Histological Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D009252:NADPH Dehydrogenase / Q000032:analysis*; D012165:Retinal Ganglion Cells / Q000648:ultrastructure*; D012171:Retinal Vessels / Q000033:anatomy & histology
D009252:NADPH Dehydrogenase