Two-colour combination enzyme-linked immunosorbent assay for the simultaneous detection of HBV and HIV infection.
To reduce the cost, time and waste in screening for HIV and HBV infections a combined assay for HIV-1 and -2 antibodies and HBsAg has been developed. Monoclonal anti-HBs antibodies were co-immobilized with synthetic peptides representing immunodominant regions of HIV-1 and -2. The presence of anti-HIV antibodies in the samples was detected with alkaline phosphatase-labelled anti-human IgG and of HBsAg with horseradish peroxidase-labelled monoclonal anti-HBs antibodies by a sequential substrate reaction. In this assay, HBsAg was detectable in a concentration range between 0.25 and 0.30 U/ml and the results were available within 3 h. The specificity, tested on 5000 serum samples from blood donors after confirmation, was 99.8% for HBsAg and 99.5% for anti-HIV antibody detection. All serum samples taken from 600 HIV-1- and 115 HIV-2-infected individuals were correctly classified as reactive. The two-colour HBsAg-anti-HIV-1/-2 combination ELISA meets all the requirements of single parameter assays with regard to precision, stability and robustness.
Porstmann
T
T
Department of Medical Immunology, Medical School Charité, Humboldt University of Berlin, Germany.
Nugel
E
E
Henklein
P
P
Döpel
H
H
Rönspeck
W
W
Pas
P
P
von Baehr
R
R
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D000911:Antibodies, Monoclonal; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D006509:Hepatitis B / Q000175:diagnosis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D006514:Hepatitis B Surface Antigens