Transformation of four pathogenic Phytophthora spp by microprojectile bombardment on intact mycelia.
Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the beta-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric beta-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.
Bailey
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AM
Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados (CINVESTAV), Guanajuato, Mexico.
Mena
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GL
Herrera-Estrella
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L
D015139:Blotting, Southern; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers; D005821:Genetic Techniques; D005966:Glucuronidase / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology; D010838:Phytophthora / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D010957:Plasmids; D014170:Transformation, Genetic; D014414:Tungsten
D004274:DNA, Recombinant; D005819:Genetic Markers; D006921:Hygromycin B; D005966:Glucuronidase; D014414:Tungsten