Immunodetection of low concentrations of ovalbumin in cryoprocessed quail oviduct cells by semi-automatic quantitative analysis.
In a previous paper (Quintana et al, Biol Cel 72, (1991) 167-180) we reported the anti-ovalbumin antibody immunolabeling in the cytoplasm of tubular gland cells from cryofixed, cryosubstituted and acrylic-resin embedded quail oviduct. To confirm these preliminary visual observations, we have carried out a semi-automatic quantitative study of immunogold labeling. The quantitative results confirm the previous data. In addition, we found a significant higher immunolabeling with low temperature Lowicryl K11M embedding procedure as compared with high temperature LR White embedding.
Quintana
C
C
U303 INSERM, La Darse, Villefranche-sur-mer, France.
López-Iglesias
C
C
Bonnet
N
N
Lebonvallet
S
S
D000818:Animals; D001322:Autoanalysis; D015925:Cryopreservation; D007150:Immunohistochemistry; D010047:Ovalbumin / Q000032:analysis*; D010057:Oviducts / Q000737:chemistry*; D011784:Quail / Q000378:metabolism*; D016610:Tissue Embedding
D010047:Ovalbumin