title: Functional domains of c-Myc involved in the commitment and differentiation of murine erythroleukemia cells.
abstract: In the early event of the induction of mouse erythroleukemia (MEL) cell differentiation, c-myc mRNA levels show a drastic change. The elevated expression of a transfected c-myc gene inhibits the commitment and differentiation of MEL cell transformants. In the present work, we have introduced human c-myc mutants into MEL cells under the inducible promoter to define the functional domains of c-Myc involved in erythroid differentiation. The c-Myc domains necessary for commitment and differentiation are not co-localized; almost entire regions are required for inhibition of commitment, whereas domains II and IV that are essential for co-transforming activity with ras are required for inhibition of differentiation. Interestingly, mutants that delete domains for c-Myc dimerization motifs enhanced differentiation. These results suggest that c-Myc may regulate commitment and differentiation by interacting with proteins through different domains.
authors.affiliation: Department of Cell Biology, Tohoku University, Sendai, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohmori
authors.forename: J
authors.initials: J
authors.lastname: Tanabe
authors.forename: S
authors.initials: S
authors.lastname: Takada
authors.forename: W M
authors.initials: WM
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Obinata
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D016259:Genes, myc; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D009154:Mutation; D016160:Retinoblastoma Protein / Q000378:metabolism; D014162:Transfection; D015032:Zinc / Q000494:pharmacology
chemical_list: D016160:Retinoblastoma Protein; D015032:Zinc