Modulation of glycogen metabolism of rat skeletal muscles by endurance training and testosterone treatment.
The effects of training and/or testosterone treatment on glycogen content and the activities of glycogen synthase, glycogen phosphorylase, and fructose-6-phosphate kinase were studied in extensor digitorum longus (EDL) and soleus muscles of intact adult female rats. One group of rats remained sedentary, whereas another group was trained for 7 weeks. Thereafter, both the sedentary and trained rats were subdivided into two control and four testosterone-treated subgroups. Testosterone was administered by a silastic implant. Training was continued for 2 weeks. On the final day of the experiment rats from one trained control and one trained testosterone-treated subgroup ran for 60 min submaximally. Upon testosterone treatment of sedentary rats the glycogen concentration was not changed. However, in the soleus, but not in the EDL, the glycogen content was increased by training (P < 0.05) which could, at least partly, be explained by a decrease in activity of active glycogen phosphorylase (P < 0.05). In the EDL of trained rats testosterone treatment increased glycogen content significantly by both an increase in activity of active glycogen synthase and a decrease in activity of active glycogen phosphorylase (P < 0.05). In the EDL and soleus of testosterone-treated animals from the exercised subgroup a significant sparing of glycogen was observed, which could be explained by an increase in activity of active glycogen synthase and, in the soleus, could also be explained by a concerted decrease in active glycogen phosphorylase (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
van Breda
E
E
Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), The Netherlands.
Keizer
H A
HA
Geurten
P
P
van Kranenburg
G
G
Menheere
P P
PP
Kuipers
H
H
Glatz
J F
JF
D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D006006:Glycogen Synthase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010732:Phosphofructokinase-1 / Q000378:metabolism; D006005:Phosphorylases / Q000378:metabolism; D010805:Physical Conditioning, Animal; D010807:Physical Endurance / Q000502:physiology; D051381:Rats; D011914:Rats, Inbred BN; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*
D013739:Testosterone; D006003:Glycogen; D006005:Phosphorylases; D006006:Glycogen Synthase; D010732:Phosphofructokinase-1; D005947:Glucose