Immunocytochemical characterization of p24, a baculovirus capsid-associated protein.
An open reading frame (ORF 1) located upstream of the polyhedron envelope protein gene in the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpMNPV) genome was cloned in-frame into a trpE bacterial expression vector. The fusion protein produced by this construct was used for the preparation of a monospecific antiserum. Western blot analysis of extracts from OpMNPV-infected Lymantria dispar cells and Autographa californica NPV (AcMNPV)-infected Spodoptera frugiperda cells detected a 24K protein late in infection. This antiserum also reacted with a 24K protein in preparations of budded and polyhedra-derived virus from OpMNPV and AcMNPV. The 24K protein was not N-glycosylated. Immunoelectron microscopy confirmed that the OpMNPV p24 is associated with nucleocapsids of budded and polyhedra-derived virions.
Wolgamot
G M
GM
Department of Agricultural Chemistry, Agricultural and Life Sciences, Oregon State University, Corvallis 97331-7301.
Gross
C H
CH
Russell
R L
RL
Rohrmann
G F
GF
D016367:Baculoviridae / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry*; D015870:Gene Expression; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis* / Q000235:genetics
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins