The antibacterial activity of haemin compared with cobalt, zinc and magnesium protoporphyrin and its effect on potassium loss and ultrastructure of Staphylococcus aureus.
The unique antibacterial properties of Fe-protoporphyrin (haemin) on Staphylococcus aureus, compared to Co-protoporphyrin (Co-PP), Mg-protoporphyrin (Mg-PP) and Zn-protoporphyrin (Zn-PP) are described. Only haemin (20 microM) exhibits a strong light-independent antibacterial effect on S. aureus; the other metalloporphyrins, Co-PP, Mg-PP or Zn-PP, have no antibacterial effect in the dark. Only light photosensitization of Mg-PP-treated cells resulted in the inhibition of the bacterial growth, while Co-PP or Zn-PP were photodynamically inactive. A notable effect of haemin on inactivation of S. aureus was the induction of immediate ion fluxes as determined by X-ray microanalysis (XRMA) of fast-frozen cells. A marked efflux of K (96%) and Cl (94%) was expressed immediately as determined by X-ray microanalysis of S. aureus cells treated with haemin for 5 min. Only 48% loss of Na was detected in the cells under these treatment conditions, while P content was increased by 150%. Electron microscopy analysis revealed the appearance of a mesosome-like structure connected to the new septa, filamentous chromosome and arrays of aggregated ribosomes in the cytoplasm. We propose that haemin has multiple cellular targets for its oxidative effect in S. aureus.
Ladan
H
H
Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Nitzan
Y
Y
Malik
Z
Z
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004249:DNA Damage; D004577:Electron Probe Microanalysis; D006427:Hemin / Q000494:pharmacology*; D008854:Microscopy, Electron; D011188:Potassium / Q000378:metabolism; D011524:Protoporphyrins / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D011524:Protoporphyrins; C039629:magnesium protoporphyrin; C017803:zinc protoporphyrin; C007095:cobaltiprotoporphyrin; D006427:Hemin; D011188:Potassium