The production of porphyrins from delta-aminolaevulinic acid by Haemophilus parainfluenzae.
Porphyrin production by the haemin-independent Haemophilus parainfluenzae in the diagnostic porphyrin test, which determines the X-factor requirement in Haemophilus spp., was analysed quantitatively by applying modern high-performance liquid chromatographic (HPLC) methods. Ion-pair reversed-phase HPLC enabled the simultaneous separation of all porphyrin intermediates and their isomers of haem biosynthesis produced by the bacteria. The pH-dependence of porphyrin production and the respective composition of the porphyrins within the bacterial cells and in the supernate were investigated. A pH optimum of 6.9-8.0 for the production of porphyrins was found and there were marked differences in the porphyrin profiles at different pH values.
Luppa
P
P
Institute of Clinical Chemistry, University Hospital Grosshadern, University of Munich, Germany.
Jacob
K
K
Ehret
W
W
D000622:Aminolevulinic Acid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000096:biosynthesis; D003470:Culture Media; D006190:Haemophilus / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011166:Porphyrins / Q000096:biosynthesis* / Q000302:isolation & purification; D011524:Protoporphyrins / Q000096:biosynthesis
D003306:Coproporphyrins; D003470:Culture Media; D011166:Porphyrins; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX