title: A change in soybean agglutinin binding patterns of bovine milk fat globule membrane glycoproteins during early lactation.
abstract: Milk fat globule membrane (MFGM) glycoproteins were prepared from bovine milk at different stages of early lactation. Western blot analyses using several lectins revealed that reactivity of MFGM glycoproteins, especially 47K and 80K bands, to soybean agglutinin (SBA) remarkably increased during the lactation, while no change was observed for Ricinus communis agglutinin-I (RCA-I) binding. Sialidase treatment of MFGM glycoproteins revealed that the number of SBA-positive bands and the amount of SBA-positive oligosaccharides in these bands are increased during the lactation. Since SBA binds N-acetylgalactosamine terminated oligosaccharides, the results indicated that N-acetylgalactosaminylation of bovine MFGM glycoproteins is stimulated during the lactation.
authors.affiliation: Department of Applied Biological Sciences, School of Agricultural Science, Nagoya University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ujita
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: N
authors.initials: N
authors.lastname: Aoki
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: A
authors.initials: A
authors.lastname: Noda
authors.forename: R
authors.initials: R
authors.lastname: Nakamura
authors.forename: D E
authors.initials: DE
authors.lastname: Greenwalt
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002417:Cattle; D002854:Chromatography, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007774:Lactation; D037102:Lectins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D018396:Mucin-1; D037121:Plant Lectins; D030262:Soybean Proteins; D013025:Glycine max / Q000378:metabolism; D010835:Phytohemagglutinins
chemical_list: D037102:Lectins; D008562:Membrane Glycoproteins; D018396:Mucin-1; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins