title: Rate of 59Fe uptake into brain and cerebrospinal fluid and the influence thereon of antibodies against the transferrin receptor.
abstract: Uptake of 59Fe from blood into brains of anaesthetized rats and mice has been studied by intravenous infusion of [59Fe]ferrous ascorbate or of 59Fe-transferrin, the results not being significantly different. Uptakes in the rat were linear with time, but increased at longer times in the mouse. Transfer constants, K(in) (in ml/g/h x 10(3)), for cerebral hemispheres were 5.2 in the adult rat and 5.6 in the mouse. These K(in) values corresponded to 59Fe influxes of 145 and 322 pmol/g/h, respectively. 59Fe uptake into the mouse brain occurred in the following order: cerebellum > brainstem > frontal cerebral cortex > parietal cortex > occipital cortex > hippocampus > caudate nucleus. In genetically hypotransferrinaemic mice, 59Fe uptake into brain was 80-95 times greater than in To strain mice. Pretreatment of young rats and mice with monoclonal antibodies to transferrin receptors, i.e., the anti-rat immunoglobulin G OX 26 and the anti-mouse immunoglobulin M RI7 208, inhibited 59Fe uptake into spleen by 94% and 98%, respectively, indicating saturation of receptors. The antibodies reduced 59Fe uptake into rat brain by 35-60% and that into mouse brain by 65-85%. Although a major portion of iron transport across the blood-brain barrier is normally transferrin-mediated, non-transferrin-bound iron readily crosses it at low serum transferrin levels.
authors.affiliation: Biomedical Sciences Division (Physiology), King's College, London, England.
authors.forename: F
authors.initials: F
authors.lastname: Ueda
authors.forename: K B
authors.initials: KB
authors.lastname: Raja
authors.forename: R J
authors.initials: RJ
authors.lastname: Simpson
authors.forename: I S
authors.initials: IS
authors.lastname: Trowbridge
authors.forename: M W
authors.initials: MW
authors.lastname: Bradbury
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood; D007501:Iron / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics*; D007504:Iron Radioisotopes; D033862:Iron-Binding Proteins; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D051381:Rats; D017208:Rats, Wistar; D011990:Receptors, Transferrin / Q000276:immunology*; D013154:Spleen / Q000378:metabolism; D014168:Transferrin / Q000032:analysis / Q000172:deficiency; D033863:Transferrin-Binding Proteins
chemical_list: D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007504:Iron Radioisotopes; D033862:Iron-Binding Proteins; D011990:Receptors, Transferrin; D014168:Transferrin; D033863:Transferrin-Binding Proteins; D007501:Iron