title: Structure of the cDNA coding for conglutin gamma, a sulphur-rich protein from Lupinus angustifolius.
abstract: The sequence of cDNA coding for a sulphur-rich storage protein from Lupinus angustifolius L., conglutin gamma, was determined. The coding region contained an N-terminal leader peptide of 28 amino acids which directly preceded subunits of M(r) 28,239 and 16,517. Extensive sequence homology between the protein encoded by conglutin gamma cDNA and basic 7S globulin from soybean was observed. Sequence homology to proteins from other classes of storage proteins, 11S, 7S and 2S, was limited to short and highly fragmented sequences. The amino acid sequence, Asn-Gly-Leu-Glu-Glu-Thr, characteristic of the primary site for post-translational cleavage of the precursors of 11S proteins, was absent from the sequence predicted for prepro-conglutin gamma. It is concluded that conglutin gamma is a representative of a fourth type of storage protein in legumes, distinct from the 11S, 7S and 2S storage protein families.
authors.affiliation: Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Vic, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Kolivas
authors.forename: K R
authors.initials: KR
authors.lastname: Gayler
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D002523:Edible Grain / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010946:Plants, Medicinal; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D012984:Software
chemical_list: D010940:Plant Proteins; C079483:conglutin-gamma protein, Lupinus angustifolius; D004247:DNA