Diagnosis of childhood BK virus cystitis by electron microscopy and PCR.
A case of BK virus cystitis in a 5 year old boy is reported. This patient, who was not immunocompromised, had had acute cystitis for two weeks. Many intracytoplasmic inclusions were observed in urinary sediment smears stained by the Papanicolaou method. Electron microscopic examination showed virus particles, presumed to be human polyomavirus, in the nuclei of the degenerated urothelial cells. A DNA sequence of the BK virus was detected in 200-300 urothelial cells in Papanicolaou stained smears by the polymerase chain reaction. BK virus is an unusual cause of symptomatic cystitis in a healthy child.
Saitoh
K
K
Department of Pathology, National Medical Center, Tokyo, Japan.
Sugae
N
N
Koike
N
N
Akiyama
Y
Y
Iwamura
Y
Y
Kimura
H
H
D001739:BK Virus; D002675:Child, Preschool; D003556:Cystitis / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000175:diagnosis*; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000473:pathology; D001743:Urinary Bladder / Q000648:ultrastructure
D004279:DNA, Viral