Post-translational cleavage of a histone H1-like protein in the sperm of Mytilus.
Starting with total RNA from spermatogenic cells of Mytilus trossulus and using random priming, we have cloned and sequenced the c-DNAs corresponding to two variants of the sperm-specific protein PLII* (phi 2B). DNA sequencing in conjunction with mass spectrometry and protein sequence data have allowed us to establish that of the three sperm-specific proteins present in the sperm of Mytilus (PL-II*(phi 2B), PL-III (phi 1), PL-IV (phi 3)), the first and the last one are the result of post-translational cleavage of a common precursor. This common precursor is a member of the histone H1 family, and it exhibits inter- and intraspecific microheterogeneity.
Carlos
S
S
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
Hunt
D F
DF
Rocchini
C
C
Arnott
D P
DP
Ausio
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics*; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D013094:Spermatozoa / Q000378:metabolism*
D004268:DNA-Binding Proteins; D006657:Histones; D009687:Nuclear Proteins; C068699:PL-II protein, Mytilus