title: Simulated quantitative and qualitative isotachophoretic indices of 73 amino acids and peptides in the pH range 6.4-10.
abstract: Qualitative and quantitative isotachophoretic indices of 73 amino acids, dipeptides and tripeptides were simulated under 24 leading electrolyte conditions covering the pH range 6.4-10. The RE values and time-based zone lengths are tabulated together with the absolute mobility (m0) and pKa values used. The leading electrolyte used was 10 mM HCl and the pH buffers were imidazole, tris(hydroxymethylamino)methane, 2-amino-2-methyl-1,3-propanediol and ethanolamine. The simulated indices will be useful in the assessment of the separability and determination of the listed and related compounds.
authors.affiliation: Faculty of Engineering, Hiroshima University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hirokawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kiso
authors.forename: B
authors.initials: B
authors.lastname: Gas
authors.forename: I
authors.initials: I
authors.lastname: Zusková
authors.forename: J
authors.initials: J
authors.lastname: Vacík
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D002021:Buffers; D004573:Electrolytes; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010455:Peptides / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D002021:Buffers; D004573:Electrolytes; D010455:Peptides