Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena.
The spatially patterned differentiation of heterocysts in the filamentous cyanobacterium Anabaena requires a functional hetR gene. Transcriptional fusions to luxAB show that hetR is transcribed at a low level throughout the filament when Anabaena is grown with combined nitrogen, and that induction of the gene begins within 2 h following nitrogen deprivation. By 3.5 h, induction is localized to spaced foci. By 6 h, there is an overall induction of at least threefold in whole cultures, reflecting at least a 20-fold increase within spatially separated cells. The induction requires the presence of a functional hetR gene, indicating that hetR is autoregulatory. Full induction of a heterocyst structural gene, hepA, also requires a functional hetR locus.
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MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824.
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D018528:ATP-Binding Cassette Transporters; D017033:Anabaena / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D005246:Feedback; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009584:Nitrogen / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C079727:hepA protein, Anabaena; C067403:HetR protein, Bacteria; D009584:Nitrogen