Neisseria weaveri sp. nov., formerly CDC group M-5, a gram-negative bacterium associated with dog bite wounds.
CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by 16S rRNA sequencing. DNA from 40 of 53 strains showed 82 to 100% relatedness (hydroxyapatite method) to labeled DNA from strain 8142. The guanine-plus-cytosine (G + C) content in 8 of the 41 highly related M-5 strains was 50.5 to 52 mol%. These 41 strains were oxidase and catalase positive, nonfermentative, nitrite positive, nitrate negative, weakly phenylalanine deaminase positive, aerobic, and alpha-hemolytic (sheep blood). DNA from the 13 remaining strains showed only 7 to 46% DNA relatedness to strain 8142. These 13 non-M-5 strains differed from the M-5 strains in G + C content, growth characteristics, and biochemical profiles. DNA from M-5 strain 8142 was most closely related to DNA from groups EF-4b (47%) and EF-4a (45%). 16S rRNA sequence analysis placed M-5 strain 8142 in the Neisseriaceae cluster of the beta-3 subgroup of the class Proteobacteria. It was most homologous (98.4 to 98.8%) to Neisseria animalis, Neisseria flavescens, Neisseria canis, and Neisseria elongata. All data are consistent with M-5 being a new species of Neisseria, for which we propose the name Neisseria weaveri.
Andersen
B M
BM
Department of Medical Microbiology, University Hospital of Tromsø, Norway.
Steigerwalt
A G
AG
O'Connor
S P
SP
Hollis
D G
DG
Weyant
R S
RS
Weaver
R E
RE
Brenner
D J
DJ
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D001733:Bites and Stings / Q000150:complications*; D004269:DNA, Bacterial / Q000032:analysis; D004285:Dogs; D008969:Molecular Sequence Data; D009343:Neisseria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016870:Neisseriaceae Infections / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D014946:Wound Infection / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S