Effect of chlorpromazine on kinetics of injected monoclonal antibody in MoAb-induced glomerular injury.
The effect of chlorpromazine, one of several calmodulin antagonists that inhibit cytoskeletal movement, on the local kinetics of injected proteinuria-inducing MoAb 5-1-6 was examined to test the hypothesis that proteinuria is inhibited if the antigen recognized by MoAb 5-1-6 or injected MoAb remains on the surface of epithelial foot processes. MoAb 5-1-6 was injected into both chlorpromazine-treated (5 mg/100 g body weight) and untreated rats. As a positive control for the chlorpromazine treatment, anti-Fx1A serum was also injected into other chlorpromazine-treated and untreated rats. Chlorpromazine inhibited neither the change in localization of injected MoAb 5-1-6 nor proteinuria, although it showed an inhibitory effect on redistribution of immune complex and the fixation of complement in passive Heymann glomerulonephritis induced by injection of anti-Fx1A serum. We conclude that the kinetics of bound MoAb 5-1-6 are regulated by a system different from that operating in passive Heymann glomerulonephritis.
Takashima
N
N
Department of Immunology, Niigata University School of Medicine, Japan.
Kawachi
H
H
Oite
T
T
Nishi
S
S
Arakawa
M
M
Shimizu
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002746:Chlorpromazine / Q000494:pharmacology*; D005260:Female; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology*; D016253:Microscopy, Immunoelectron; D011507:Proteinuria / Q000209:etiology / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D002746:Chlorpromazine