Plasma and urinary oxalate and glycolate in healthy subjects.
High-performance ion chromatography (HPIC) has been widely used for oxalate analysis and, more recently, for glycolate analysis. We describe a procedure for sample preparation in which the plasma ultrafiltrate is acidified during harvesting with a cation-exchange resin, and the chloride is removed before the ion chromatography, which is performed with a newly developed AS10 column. The same ultrafiltrate sample is analyzed for glycolate. For plasma oxalate, the mean recovery of sample in eluted fractions was 95-96%, and intraassay CV was 6.2-8.1%. The reference interval (mean +/- 2 SD) for men was 0.8-3.2 mumol/L and for women, 1.0-2.6 mumol/L. For urinary oxalate, the reference interval for men was 175-560 mumol/day and for women, 107-432 mumol/day. For plasma glycolate, the mean analytical recovery was 96-98%, and the intra-assay CV was 2.4-6.2%. The reference interval for men was 1.9-7.5 mumol/L and for women, 1.4-7.4 mumol/L. For urinary glycolate, the reference interval for men was 0-1400 mumol/day and for women, 91-1001 mumol/day.
Hagen
L
L
Department of Medicine, University of British Columbia, Vancouver, Canada.
Walker
V R
VR
Sutton
R A
RA
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D006016:Glycolates / Q000097:blood* / Q000652:urine*; D006801:Humans; D008297:Male; D010070:Oxalates / Q000097:blood* / Q000652:urine*; D019815:Oxalic Acid; D012016:Reference Values
D006016:Glycolates; D010070:Oxalates; C031149:glycolic acid; D019815:Oxalic Acid