title: A NotI restriction map of the entire long arm of human chromosome 21.
abstract: A variety of maps of the human genome have been constructed, including cloned DNA maps. We have isolated 40 of the 42 NotI sites that exist on the long arm of human chromosome 21, as NotI linking clones and constructed a complete NotI restriction map spanning the entire region. This map, which provides the most reliable ordering and distance estimation in the region from a pericentromeric locus to the terminus, demonstrates the usefulness of linking clone mapping for analysing human chromosomes.
authors.affiliation: Department of Immunology and Virology, Saitama Cancer Center Research Institute, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ichikawa
authors.forename: F
authors.initials: F
authors.lastname: Hosoda
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: M
authors.initials: M
authors.lastname: Ohira
authors.forename: M
authors.initials: M
authors.lastname: Ohki
mesh_terms: D000818:Animals; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008040:Genetic Linkage; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D015183:Restriction Mapping
chemical_list: D005819:Genetic Markers; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases