title: Mitomycin-induced synthesis of a Shiga-like toxin from enteropathogenic Escherichia coli H.I.8.
abstract: Escherichia coli H.I.8, an O128 infant diarrhea isolate, produces low titers of a unique Shiga-like toxin (SLT), called SLT-IIva, which is a variant of SLT-II. We investigated induction of toxin synthesis and the putative association of a bacteriophage with toxin synthesis. Induction of broth cultures of strain H.I.8 with mitomycin yielded a 3,000-fold increase in SLT-IIva, production of a colicin, and appearance of a bacteriophage. Southern hybridization demonstrated that the genes for SLT-IIva were not carried by the bacteriophage.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
authors.forename: A J
authors.initials: AJ
authors.lastname: Yee
authors.forename: S
authors.initials: S
authors.lastname: De Grandis
authors.forename: C L
authors.initials: CL
authors.lastname: Gyles
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis*; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D016685:Mitomycin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2
chemical_list: D001427:Bacterial Toxins; D009838:Oligodeoxyribonucleotides; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D016685:Mitomycin