[Preliminary study on monoclonal antibodies against Cysticercus cellulosae].
In order to produce valuable monoclonal antibodies for immunodiagnosis of cysticercosis, hybridoma technique was adopted using the fusion of splenic lymphocytes from BALB/c mice immunized with Cysticercus cellulosae antigens and the myeloma cell line NS-1. Through screening and cloning, three monoclonal antibody cell lines have been established. Immunoglobulin subclasses of 3 lines were determined to be IgG1. These monoclonal antibodies were found to be highly specific and without cross reaction with Echinococcus granulosus hydatid cyst and Cysticercus fasciolasis. Antigen fractions specifically reacting to monoclonal antibodies were demonstrated with Western blot method. The results showed that specific reaction bands of SCW located in 25 kDa and 17 kDa regions.
Li
S H
SH
Department of parasitology, Norman Bethune University of Medical Sciences, Changchun.
Du
J
J
Lian
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D015153:Blotting, Western; D002459:Cell Fusion; D003552:Cysticercus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006825:Hybridomas / Q000378:metabolism*; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G