Mobility of the maize transposable element En/Spm in Arabidopsis thaliana.
The autonomous element En-1 of the maize En/Spm transposable element system is capable of frequent somatic and germinal excision in the heterologous host Arabidopsis thaliana. The pattern of En-homologous transcripts generated in transgenic Arabidopsis resembles En transcription in maize. An excision reporter construct based on NPT-II gene (pKEn2) can be used reliably for the isolation of En-1 germinal revertants by seed germination on kanamycin-containing medium. Re-insertion after germinal excision is apparently frequent. A dSpm receptor element can be efficiently trans-activated in Arabidopsis either by En-1 or by expressing cDNAs of tnpA and tnpD. Excision and re-insertion of En/Spm take place with similar characteristics as in maize. This is the first description of En/Spm transposition in Arabidopsis and the parameters analysed here suggest that transposon tagging with En should be feasible in this species.
Cardon
G H
GH
Max-Planck-Institut für Züchtungsforschung, Abteilung Molekulare Pflanzegenetik, Köln, Germany.
Frey
M
M
Saedler
H
H
Gierl
A
A
D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D004247:DNA; D004251:DNA Transposable Elements; D007612:Kanamycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D010641:Phenotype; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D007612:Kanamycin; D004247:DNA