title: Cell proteins bind to sites within the 3' noncoding region and the positive-strand leader sequence of measles virus RNA.
abstract: The genomic 3' noncoding region (NCR) of nonsegmented negative-strand RNA viruses contains recognition site(s) for the polymerase complex, while the RNA plus-strand leader sequence (LS) is probably involved in RNA encapsidation. It is known that host-encoded factors play a role in transcription and replication of some of this group of viruses. Here we report that cellular proteins interact with the genomic 3' NCR and with the plus-strand LS RNA of an important human pathogen, measles virus (MV), a member of the family Paramyxoviridae. Using gel retardation assay and RNA footprinting analysis, we demonstrated that in Vero cells, host-encoded proteins bind specifically to domains within these two sequences. A polypeptide of about 20 kDa binding to the 3' NCR and two polypeptides of about 22 and 30 kDa interacting with plus-strand LS were detected by RNA-protein UV cross-linking. Different RNA-binding activities were found in cells differing in permissiveness to MV replication. The results suggest a role for host-encoded proteins in MV replication.
authors.affiliation: Department of Virology, University of Turku, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Leopardi
authors.forename: V
authors.initials: V
authors.lastname: Hukkanen
authors.forename: R
authors.initials: R
authors.lastname: Vainionpää
authors.forename: A A
authors.initials: AA
authors.lastname: Salmi
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D004274:DNA, Recombinant; D008459:Measles virus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D014466:Ultraviolet Rays
chemical_list: D004274:DNA, Recombinant; D009698:Nucleoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins