title: Effect of metal ions on the activity of casein kinase II from Xenopus laevis.
abstract: Casein kinase II purified from the nuclei of Xenopus laevis oocytes as well as the recombinant alpha and beta subunits of the X. laevis CKII, produced in E. coli from the cloned cDNA genes, were tested with different divalent metal ions. The enzyme from both sources was active with either Mg2+, Mn2+, or Co2+. Optimal concentrations were 7-10 mM for Mg2+, 0.5-0.7 mM for Mn2+ and 1-2 mM for Co2+. In the presence of Mn2+ or Co2+ the enzyme used GTP more efficiently than ATP as a phosphate donor while the reverse was true in the presence of Mg2+. The apparent Km values for both nucleotide triphosphates were greatly decreased in the presence of Mn2+ as compared with Mg2+. Addition of Zn2+ (above 150 microM) to an assay containing the optimal Mg2+ ion concentration caused strong inhibition of both holoenzyme and alpha subunit. Inhibition of the holoenzyme by 400 microM Ni2+ could be reversed by high concentrations of Mg2+ but no reversal of this inhibition was observed with the alpha subunit.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.
authors.forename: M
authors.initials: M
authors.lastname: Gatica
authors.forename: M V
authors.initials: MV
authors.lastname: Hinrichs
authors.forename: A
authors.initials: A
authors.lastname: Jedlicki
authors.forename: C C
authors.initials: CC
authors.lastname: Allende
authors.forename: J E
authors.initials: JE
authors.lastname: Allende
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D047390:Casein Kinase II; D002413:Cations, Divalent; D003035:Cobalt / Q000378:metabolism; D005260:Female; D006160:Guanosine Triphosphate / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D010053:Ovary / Q000201:enzymology; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D008670:Metals; D003035:Cobalt; D008345:Manganese; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D008274:Magnesium; D015032:Zinc