Glycan microheterogeneity of alpha 1-antitrypsin in serum and meconium from normal and cystic fibrosis patients by crossed immunoaffinoelectrophoresis with different lectins (Con A, LCA, WGA).
In order to test whether abnormalities of glycosylation occur in cystic fibrosis (CF), the glycan microheterogeneity of alpha 1-antitrypsin (alpha 1-AT) was studied in serum and meconium from normal individuals and patients with cystic fibrosis, by crossed immunoaffinoelectrophoresis (CIAE) using free Concanavalin A (Con A), Lens culinaris lectin (LCA) and wheat germ agglutinin (WGA). Three main results emerged from this study: (1) modification of glycosylation in serum alpha 1-AT from patients with cystic fibrosis were only significant with free Con A and WGA; this probably results from a reduced synthesis of the bi-antennary side-chains or by their increased catabolism. (2) Differences in isoforms found in alpha 1-AT from normal individuals and patients with CF using free Con A, LCA, were more pronounced in the meconium than in the serum; this may provide a useful test in diagnosis of cystic fibrosis. (3) There was parallelism between the behaviour of alpha 1-AT in serum and meconium from patients with CF using LCA, Con A; this may be explained by different types or levels of disfunction affecting a glycosylation mechanism.
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Biochemistry Laboratory, Centre Hospitalier Lyon-Sud, Pierre-Bénite, France.
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D000328:Adult; D003208:Concanavalin A; D003550:Cystic Fibrosis / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods*; D007231:Infant, Newborn; D037102:Lectins; D008470:Meconium / Q000737:chemistry*; D037121:Plant Lectins; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*; D014909:Wheat Germ Agglutinins; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000378:metabolism*
D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides; D014909:Wheat Germ Agglutinins; D000515:alpha 1-Antitrypsin; C033619:lentil lectin; D003208:Concanavalin A