Distribution of beta-adrenergic binding in fractionated porcine adipocytes.
1. The subcellular distribution of the porcine adipocyte beta-adrenergic receptor was studied in fractionated adipocytes. 2. The 30,000 g pellet obtained from hypotonically lysed cells contained membrane vesicles and mitochondria; it yielded approx 200-300 fmol dihydroalprenolol-bound receptors/mg protein. 3. Activity was increased to about 1000 fmol/mg protein after isolation of a plasma membrane fraction on a Percoll gradient. 4. The 5'-nucleotidase, succinate dehydrogenase and lactate dehydrogenase activities were usually enriched in compartments different from the ligand-binding activity. 5. Activity of porcine adipocyte 5'-nucleotidase, a purported plasma membrane marker enzyme, was not distributed in the same manner as the beta-adrenergic receptor.
Mersmann
H J
HJ
Department of Pediatrics, Baylor College of Medicine, Houston, Texas.
McNeel
R L
RL
Heath
J P
JP
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D002498:Centrifugation / Q000379:methods; D008297:Male; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism*; D013552:Swine
D011943:Receptors, Adrenergic, beta