Determination of the heroin metabolite 6-monoacetyl-morphine in urine by high-performance liquid chromatography with electrochemical detection.
A procedure has been developed for detection and quantitation of 6-monoacetylmorphine (6-MAM), the specific metabolite of heroin in human urine. 6-MAM was isolated by solid-phase extraction, and the extracts were analyzed by high-performance liquid chromatography with electrochemical detection in the oxidizing mode. Good linearity was obtained in the concentration range 10-250 ng/mL. Concentrations of 2 ng 6-MAM/mL can be detected using a 2-mL sample of urine. The method has been verified in urine samples of heroin abusers.
Hanisch
W
W
Institut für Rechtsmedizin, München, Germany.
Meyer
L V
LV
D002851:Chromatography, High Pressure Liquid; D003932:Heroin / Q000378:metabolism; D006801:Humans; D009022:Morphine Derivatives / Q000652:urine*; D010084:Oxidation-Reduction; D015813:Substance Abuse Detection / Q000379:methods*
D009022:Morphine Derivatives; D003932:Heroin; C026979:6-O-monoacetylmorphine