Crystallization of pancreatic procolipase and of its complex with pancreatic lipase.
The human pancreatic lipase-porcine procolipase complex has been crystallized in space group P3(2)21 (a = b = 80.3 A and c = 251 A) from a solution containing polyethylene glycol, NaCl and beta-octyl glucoside. The crystals diffract to 2.6 A on a synchrotron beam. The complex in the presence of bile salts and phospholipids crystallizes in a tetragonal space group P4(2)2(1)2 (a = b = 133.4 A, c = 92.6 A). Crystals of procolipase alone were obtained under slightly different experimental conditions (space group I432, a = b = c = 164.3 A).
van Tilbeurgh
H
H
Laboratoire de Cristallographie et de Cristallisation des Macromolécules Biologiques, URA1296-CNRS, Faculté de Médecine Secteur-Nord, Marseille, France.
Gargouri
Y
Y
Dezan
C
C
Egloff
M P
MP
Nésa
M P
MP
Ruganie
N
N
Sarda
L
L
Verger
R
R
Cambillau
C
C
D000818:Animals; D003089:Colipases / Q000737:chemistry*; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors; D006801:Humans; D008049:Lipase / Q000737:chemistry*; D010179:Pancreas / Q000201:enzymology*; D011498:Protein Precursors / Q000737:chemistry*; D013552:Swine; D014961:X-Ray Diffraction
D003089:Colipases; D004792:Enzyme Precursors; D011498:Protein Precursors; C037107:procolipase; D008049:Lipase