Strong genetic association between HLA-DR3 and a polymorphic variation in the regulatory region of the HSP70-1 gene.
A three-allele polymorphic system is detectable by direct electrophoretic analysis of the amplified 5' untranslated und 5' flanking regions of the HLA-linked HSP70-1 gene. Single nucleotide differences at two sites, i.e., -110 and +120, are responsible for changes in the bending pattern and, consequently, in the electrophoretic mobility of the three alleles. The presence of the sequence AAACCCC around position -110 is strongly associated with DR3.
Cascino
I
I
Institute of Cell Biology, CNR, Rome, Italy.
Sorrentino
R
R
Tosi
R
R
D001483:Base Sequence; D005809:Genes, Regulator; D008040:Genetic Linkage; D015803:HLA-DR3 Antigen / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D015803:HLA-DR3 Antigen; D006360:Heat-Shock Proteins