Cloning and characterization of the C. elegans histidyl-tRNA synthetase gene.
In this paper, we report the cloning and sequencing of the C. elegans histidyl-tRNA synthetase gene. The complete genomic sequence, and most of the cDNA sequence, of this gene is now determined. The gene size including flanking and coding regions is 2230 nucleotides long. Three small introns (45-50 bp long) are found to interrupt the open reading frame. The open reading frame translates to 523 amino acids. This putative protein sequence shows extensive homology with the human and yeast histidyl-tRNA the histidyl-tRNA synthetase gene is a single copy gene. Hence, it is very likely that it encodes both the cytoplasmic and the mitochondrial histidyl-tRNA synthetases. It is likely to be trans-spliced since it contains a trans-splice site in its 5' untranslated region.
Amaar
Y G
YG
Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.
Baillie
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DL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006643:Histidine-tRNA Ligase / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012326:RNA Splicing
D018076:DNA, Complementary; D006643:Histidine-tRNA Ligase