title: Ribosomal protein gene rpl5 is cotranscribed with the nad3 gene in Oenothera mitochondria.
abstract: The rpl5 ribosomal protein gene was identified in the mitochondrial genome of the higher plant Oenothera berteriana. The gene is present in a unique genomic location upstream of the gene encoding subunit 3 of the NADH dehydrogenase (nad3). Both genes are cotranscribed, and the mRNA is modified at several cytidine residues by RNA editing. Analysis of the editing profiles of both genes by direct cDNA analysis and polymerase chain reaction (PCR) revealed that not all transcripts are fully edited at all sites. Eight of the nine C to U conversions in the rpl5 reading frame are non-silent and change the deduced amino acid sequence. The genes of the prokaryotic-like cistron that includes the rpsl9, rps3, rpl16, rpl5, and rpsl4 genes, which is at least partially conserved in the mitochondrial genomes of other higher and lower plants, are dispersed in the Oenothera mitochondrial genome.
authors.affiliation: Institut für Genbiologische Forschung GmbH, Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Schuster
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D016366:Open Reading Frames; D010446:Peptide Fragments / Q000235:genetics; D010944:Plants / Q000235:genetics*; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D010446:Peptide Fragments; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C029742:ribosomal protein L5; D009245:NADH Dehydrogenase