title: A detailed colostomy procedure and its application to quantify water and nitrogen balance and urine contribution to thermobalance in broilers exposed to thermoneutral and heat-distressed environments.
abstract: A surgical procedure for the separation of avian urinary and fecal excrement, in birds as young as 3.5 wk of age, was developed and used to quantify ambient temperature effects on urine production and composition. The colostomized broilers were used in two experiments to estimate urine production, osmolality, and contribution to water, nitrogen, and thermobalance of birds exposed to thermoneutral (24 C, TN) and heat-distressed (35 C, HD) environments. Urine production averaged over three 12-h periods was greater (P < .05), at 101 versus 51 mL/12 h per kilogram of BW and osmolality lower (P < .05) at 142 versus 220 mOsm/kg urine during HD and TN, respectively, in Experiment 1; and averaged over one 12-h period 82 versus 32 mL/12 h per kilogram of BW and 136 versus 208 mOsm/kg in Experiment 2 for HD and TN, respectively. In Experiment 1, urine production per milliliter of water consumed was increased (P < .05) by HD during Periods 2 and 3 even though in both experiments the values were similar during the first 12 h. Both urinary nitrogen and osmolar losses were increased (P < .05) by 50 and 89.2%, respectively, during HD, but without effect on fecal nitrogen. Evaporative cooling of broilers was increased (P < .05) by 154% during HD. These studies provide a well-defined colostomy procedure and suggest that broiler urine production and composition as well as overall thermobalance, water, and nitrogen balance are strongly impacted by acute HD.
authors.affiliation: Department of Animal Science, Oklahoma State University, Stillwater 74078.
authors.forename: T
authors.initials: T
authors.lastname: Belay
authors.forename: K E
authors.initials: KE
authors.lastname: Bartels
authors.forename: C J
authors.initials: CJ
authors.lastname: Wiernusz
authors.forename: R G
authors.initials: RG
authors.lastname: Teeter
mesh_terms: D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology*; D002645:Chickens / Q000502:physiology* / Q000601:surgery / Q000652:urine; D003125:Colostomy / Q000379:methods / Q000662:veterinary*; D004326:Drinking / Q000502:physiology; D006358:Hot Temperature / Q000009:adverse effects*; D009584:Nitrogen / Q000652:urine; D009994:Osmolar Concentration; D014556:Urine / Q000737:chemistry
chemical_list: D009584:Nitrogen