Pre- and posttranslational regulation of beta-endorphin biosynthesis in the CNS: effects of chronic naltrexone treatment.
There appear to be two anatomically distinct beta-endorphin (beta E) pathways in the brain, the major one originating in the arcuate nucleus of the hypothalamus and a smaller one in the area of the nucleus tractus solitarius (NTS) of the caudal medulla. Previous studies have shown that these two proopiomelanocortin (POMC) systems may be differentially regulated by chronic morphine treatment, with arcuate cells down-regulated and NTS cells unaffected. In the present experiments, we examined the effects of chronic opiate antagonist treatment on beta E biosynthesis across different CNS regions to assess whether the arcuate POMC system would be regulated in the opposite direction to that seen after opiate agonist treatment and to determine whether different beta E-containing areas might be differentially regulated. Male adult rats were administered naltrexone (NTX) by various routes for 8 days (subcutaneous pellets, osmotic minipumps, or repeated intraperitoneal injections). Brain and spinal cord regions were assayed for total beta E-ir, different molecular weight immunoreactive beta-endorphin (beta E-ir) peptides, and POMC mRNA. Chronic NTX treatment, regardless of the route of administration, reduced total beta E-ir concentrations by 30-40% in diencephalic areas (the arcuate nucleus, the remaining hypothalamus, and the thalamus) and the midbrain, but had no effect on beta E-ir in the NTS or any region of the spinal cord. At the same time, NTX pelleting increased POMC mRNA levels in the arcuate to approximately 140% of control values.(ABSTRACT TRUNCATED AT 250 WORDS)
Bronstein
D M
DM
Mental Health Research Institute, University of Michigan, Ann Arbor.
Day
N C
NC
Gutstein
H B
HB
Trujillo
K A
KA
Akil
H
H
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002850:Chromatography, Gel; D008297:Male; D009271:Naltrexone / Q000494:pharmacology*; D010455:Peptides / Q000032:analysis; D011333:Pro-Opiomelanocortin / Q000235:genetics; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D013997:Time Factors; D001615:beta-Endorphin / Q000096:biosynthesis* / Q000737:chemistry
D010455:Peptides; D012333:RNA, Messenger; D009271:Naltrexone; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin