Transient recruitment of osteoclasts and expression of their function in osteopetrotic (op/op) mice by a single injection of macrophage colony-stimulating factor.
Severe deficiency of osteoclasts in op/op mice, caused by the absence of functional macrophage colony-stimulating factor (M-CSF), is cured by daily injections of purified recombinant human M-CSF (rhM-CSF). In this study, we found that a single injection of 5 micrograms rhM-CSF is enough for recruitment of osteoclasts in mutant mice. Osteoclast number increased during the period between 2 and 4 days after the single rhM-CSF injection. When YM175, a new derivative of bisphosphonate, was administered to the mice 4 days after rhM-CSF injection or later, osteoclasts disappeared by 3 days after YM175 administration. However, a significant number of osteoclasts were detected even at 3 days after YM175 administration when YM175 was administered 3 days after rhM-CSF injection or earlier. These results indicate that YM175 is cytotoxic only to functioning osteoclasts and that recruitment of osteoclasts is finished 4 days after a single rhM-CSF injection. The osteoclasts actively resorbed bone trabeculae for a prolonged period, demonstrating that M-CSF is not requisite for the functioning of mature osteoclasts.
Kodama
H
H
Department of Anatomy, Ohu University School of Dentistry, Fukushima, Japan.
Yamasaki
A
A
Abe
M
M
Niida
S
S
Hakeda
Y
Y
Kawashima
H
H
D000818:Animals; D001862:Bone Resorption; D004164:Diphosphonates / Q000494:pharmacology; D005260:Female; D016173:Macrophage Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation; D010010:Osteoclasts / Q000187:drug effects* / Q000502:physiology; D010022:Osteopetrosis / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013977:Tibia
D004164:Diphosphonates; D011994:Recombinant Proteins; C071542:cimadronate; D016173:Macrophage Colony-Stimulating Factor