Outer membrane complex proteins of Chlamydia pneumoniae.
The protein composition of the outer membrane complex (OMC) of Chlamydia pneumoniae strain AR-39 was analyzed by metabolic labeling with [35S]methionine and [35S]cysteine. Cysteine-rich proteins with molecular masses of 98, 60 doublet, 39.5 (MOMP) and 15.5 kDa were found in the OMC of C. pneumoniae. The cysteine-rich proteins of the OMCs of the three Chlamydia species showed specific reaction patterns by immunoassay and autoradiography to rabbit or turkey immune sera. Recognition of the MOMP and 60-kDa proteins of the three species was cross-reactive. However, the C. pneumoniae 98-kDa protein was recognized by anti-C. pneumoniae (AR-39) and anti-C. psittaci (TT3) immune sera. None of the immune sera recognized the 12-kDa cysteine-rich complex.
Melgosa
M P
MP
Department of Pathobiology, University of Washington, Seattle 98195.
Kuo
C C
CC
Campbell
L A
LA
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D002692:Chlamydia trachomatis / Q000737:chemistry; D016993:Chlamydophila pneumoniae / Q000737:chemistry*; D002691:Chlamydophila psittaci / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D008970:Molecular Weight; D013045:Species Specificity
D001425:Bacterial Outer Membrane Proteins; D003545:Cysteine