Characterization of the promoter of human prostaglandin H synthase-1 gene.
The gene of human prostaglandin H synthase-1 (PGHS-1,EC 1.14.99.1) was isolated from a human cosmid library and was contained in two overlapping clones. Multiple transcriptional start sites were identified. The major one located 136 bases upstream from the ATG initiation codon. The promoter region contains no canonical TATA box but possesses a high G+C content. These observations suggest that PGHS-1 gene has the characteristics of a housekeeping gene. Both 0.8 and 0.4 kb of the 5'-flanking sequence of the PGHS-1 gene can confer transcriptional activity.
Wang
L H
LH
Department of Internal Medicine, University of Texas Health Science Center, Houston 77030.
Hajibeigi
A
A
Xu
X M
XM
Loose-Mitchell
D
D
Wu
K K
KK
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015139:Blotting, Southern; D003062:Codon; D004247:DNA / Q000737:chemistry; D015246:Deoxyribonuclease EcoRI; D005091:Exons; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D003062:Codon; D004247:DNA; D011451:Prostaglandin-Endoperoxide Synthases; D015246:Deoxyribonuclease EcoRI; D015719:Single-Strand Specific DNA and RNA Endonucleases