title: Leptomonas seymouri as a model system for the analysis of gene expression in trypanosomatids.
abstract: Leptomonas seymouri, a monogenetic trypanosomatid originally isolated from Dysdercus suturellus (Hemiptera), was used to develop a reverse genetic system for trypanosomatid flagellates. In many eukaryotic cell types, reverse genetics has proven to be a powerful tool for defining structure/function relationships within genes. The mini-exon genes of trypanosomatids encode key components of all cellular mRNAs. This component is a 5' "leader" RNA that is spliced onto all mRNA precursors during mRNA formation within the cell nucleus. The data presented here indicate that structure/function relationships within the mini-exon gene can be probed using the molecular genetic system developed and characterized for L. seymouri.
authors.affiliation: Laboratory of Molecular Parasitology, Rockefeller University, New York, New York 10021.
authors.forename: V
authors.initials: V
authors.lastname: Bellofatto
authors.forename: D E
authors.initials: DE
authors.lastname: Hartree
authors.forename: J
authors.initials: J
authors.lastname: Torres-Munoz
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D003470:Culture Media; D004261:DNA Replication; D016054:DNA, Protozoan / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D018274:Electroporation; D005091:Exons; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008954:Models, Biological; D010957:Plasmids; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014170:Transformation, Genetic; D014351:Trypanosomatina / Q000235:genetics* / Q000254:growth & development; D014404:Tubulin / Q000235:genetics
chemical_list: D003470:Culture Media; D016054:DNA, Protozoan; D012333:RNA, Messenger; D014404:Tubulin; D015500:Chloramphenicol O-Acetyltransferase