title: Androgenic regulation of expression of beta-tubulin messenger ribonucleic acid in motoneurons of the spinal nucleus of the bulbocavernosus.
abstract: Expression of beta-tubulin mRNA was examined in androgen-sensitive motoneurons of the spinal nucleus of the bulbocavernosus (SNB) in adult male rats by in situ hybridization histochemistry using cDNA encoding mouse beta-tubulin. Hybridizable beta-tubulin mRNA was localized in the somata and proximal dendrites of SNB motoneurons. Removal of androgen by castration significantly reduced the expression level of beta-tubulin mRNA in the SNB motoneurons, whereas the change was prevented by testosterone treatment. On the contrary, castration or testosterone treatment did not induce any changes in the expression level of beta-tubulin mRNA in the androgen-insensitive motoneurons of the retrodorsolateral nucleus. These results suggest that androgen regulates the expression of beta-tubulin gene in the SNB motoneurons and may provide evidence for the molecular mechanisms of hormonally-induced neuronal plasticity in the SNB motoneurons.
authors.affiliation: Department of Anatomy, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.forename: S
authors.initials: S
authors.lastname: Hyodo
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D015152:Blotting, Northern; D003712:Dendrites / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D017403:In Situ Hybridization; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009919:Orchiectomy; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology; D014404:Tubulin / Q000235:genetics*
chemical_list: D000728:Androgens; D012333:RNA, Messenger; D014404:Tubulin; D013739:Testosterone