title: Hyaluronate synthase: cloning and sequencing of the gene from Streptococcus sp.
abstract: The complete nucleotide sequence of hyaluronate synthase from Streptococcus sp. and its flanking regions is presented. The gene locus was designated has. Southern-blotting results suggested that the gene was conserved in hyaluronate-producing streptococci. A putative translation-initiation codon was identified and the open reading frame consists of 1566 bp, specifying a protein of 56 kDa. Sequences resembling the promoter and ribosome-binding site of Gram-positive organisms are found upstream of the synthase. The predicted amino-acid sequence reveals the presence of a 35-residue signal peptide. The sequence has some similarity to bacterial peptide-binding proteins.
authors.affiliation: Institut für Physiologische Chemie und Pathobiochemie, Münster, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Lansing
authors.forename: S
authors.initials: S
authors.lastname: Lellig
authors.forename: A
authors.initials: A
authors.lastname: Mausolf
authors.forename: I
authors.initials: I
authors.lastname: Martini
authors.forename: F
authors.initials: F
authors.lastname: Crescenzi
authors.forename: M
authors.initials: M
authors.lastname: O'Regan
authors.forename: P
authors.initials: P
authors.lastname: Prehm
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D014453:Glucuronosyltransferase / Q000235:genetics*; D016695:Glycosyltransferases; D000076002:Hyaluronan Synthases; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000201:enzymology*; D014166:Transferases; D029867:Xenopus Proteins
chemical_list: D004269:DNA, Bacterial; D008565:Membrane Proteins; D029867:Xenopus Proteins; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases