title: Thromboxane production in copper-deficient and marginal platelets: influence of superoxide dismutase and lipid hydroperoxides.
abstract: Platelet thromboxane (TX) production was examined in response to dietary copper. Groups of eight rats were fed copper-deficient, -marginal, and -adequate diets providing 0.5, 1.7, and 7.5 micrograms Cu/g, respectively, with controlled dietary Se and vitamin E. Platelets were purified and washed by centrifugation. Separate platelet samples from each rat were challenged with 10 micrograms/ml of collagen and 1 unit/ml (27.3 nM) of thrombin in Tyrode's buffer, 2.0 mM Ca2+. Platelet copper-dependent superoxide dismutase (CuSOD) activity showed a significant depression with reduced diet copper, but platelet glutathione peroxidase activity was unaffected. Challenged platelet TX production showed a significant 1.5- to 2.5-fold increase in response to both dietary copper deficiency and marginality, with highly significant negative correlations between challenged platelet TX production and platelet CuSOD activity and between TX production and copper status (liver copper). Endogenous (unchallenged) platelet lipid hydroperoxide concentrations, measured as free fatty acid hydroperoxides by a glutathione-disulfide-specific glutathione reductase recycling assay, showed a nonsignificant 47-67% increase in copper deficiency. Pooled data showed a significant 71% increase in platelet lipid hydroperoxides in copper deficiency. Platelet TX production showed a significant correlation with endogenous lipid hydroperoxides. The results suggest that dietary copper insufficiency increases platelet TX synthesis through changes in CuSOD in a dose-responsive (diet copper and platelet CuSOD activity) manner, and that platelet TX synthesis is influenced by lipid hydroperoxides (peroxide tone).
authors.affiliation: Department of Food Science and Human Nutrition, Colorado State University, Fort Collins 80523.
authors.forename: C L
authors.initials: CL
authors.lastname: Morin
authors.forename: K G
authors.initials: KG
authors.lastname: Allen
authors.forename: M M
authors.initials: MM
authors.lastname: Mathias
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D003300:Copper / Q000172:deficiency* / Q000378:metabolism / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000097:blood*; D007700:Kinetics; D008054:Lipid Peroxides / Q000097:blood*; D008099:Liver / Q000378:metabolism; D008297:Male; D010976:Platelet Count; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood*; D013929:Thromboxane B2 / Q000097:blood*
chemical_list: D008054:Lipid Peroxides; D013929:Thromboxane B2; D003300:Copper; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase