A new approach to the immunocytochemistry of cAMP. Initial characterization of antibodies against acrolein-fixed cAMP.
A method is described to couple cyclic adenosine 3',5' monophosphate (cAMP) to a carrier protein by means of acrolein. Antibodies against this conjugate were raised in mice. These antibodies proved to be highly specific for acrolein-fixed cAMP in a gelatin model system. Slices (300 microns in thickness) from rat cerebral cortex were incubated in vitro and the dopaminergic control of adenylate cyclase activity was drug-manipulated. This manipulation was visualized by application of the cAMP-antisera on cryostat sections of the acrolein fixed slices.
De Vente
J
J
Department of Pharmacology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
Schipper
J
J
Steinbusch
H W
HW
D000171:Acrolein; D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry; D000242:Cyclic AMP / Q000032:analysis*; D005780:Gelatin; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C
D000171:Acrolein; D005780:Gelatin; D000242:Cyclic AMP