Altered regulation of cholesterol metabolism in type I diabetic women during the menstrual cycle.
This study examines the relationship of cellular cholesterol metabolism to oestrogen and progesterone during the menstrual cycle in diabetic and non-diabetic subjects. Nine premenopausal diabetic women were compared to nine non-diabetic women of the same age. Oestrogen, progesterone, lipoproteins, including lipoprotein (a) (Lp(a)) and cholesteryl ester transfer protein (CETP) were determined in serum. Cellular cholesterol content and cellular cholesterol synthesis were measured in mononuclear leucocytes. There was no significant change in serum lipoproteins including Lp(a) during the cycle in either group. CETP activity was significantly higher over the 4 weeks in the diabetic patients compared with non-diabetic subjects (mean 463 +/- 30 mumol l-1 h-1 vs 405 +/- 28 mumol l-1 h-1, p < 0.01). Serum high density lipoprotein (HDL) cholesterol was significantly lower during the 4 weeks in the diabetic patients (1.7 +/- 0.1 mmol l-1 vs 1.8 +/- 0.1 mmol-1, p < 0.05). Cellular cholesterol synthesis decreased steadily up to the third week in cells from the control subjects whereas there was no significant change in cells from diabetic patients whose cellular cholesterol synthesis was higher at week 3 compared with non-diabetic subjects (663 +/- 54 nmol mg-1 cell protein vs 432 +/- 43 nmol mg-1 cell protein, two-way interaction p < 0.05). There was a significant negative correlation between cellular cholesterol synthesis and serum oestrogen in the non-diabetic subjects (p < 0.05) but not in the diabetic patients.(ABSTRACT TRUNCATED AT 250 WORDS)
Owens
D
D
Department of Clinical Medicine, Trinity College, Dublin, Ireland.
Cox
M
M
Caird
J
J
Gilligan
S
S
Collins
P
P
Johnson
A
A
Tomkin
G H
GH
D000085:Acetates / Q000097:blood; D000328:Adult; D000704:Analysis of Variance; D002352:Carrier Proteins / Q000097:blood*; D002784:Cholesterol / Q000096:biosynthesis / Q000097:blood*; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004967:Estrogens / Q000097:blood; D005260:Female; D006023:Glycoproteins; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000097:blood; D008597:Menstrual Cycle / Q000097:blood*; D011374:Progesterone / Q000097:blood; D012016:Reference Values
D000085:Acetates; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters; D004967:Estrogens; D006023:Glycoproteins; D008075:Lipoproteins, HDL; D011374:Progesterone; D002784:Cholesterol