Analysis of a temperature-sensitive mutation affecting the integration protein of Moloney murine leukemia virus.
We have previously described a series of mutations in the 3' terminus of the pol gene of Moloney murine leukemia virus which adversely affect the establishment of an integrated provirus (Roth, M. J., Schwartzberg, P., Tanese, N., and Goff, S. P., J. Virol. 64, 4709-4717, 1990). While most of the mutations were unconditionally lethal for virus replication, we now demonstrate that one mutation, in6161-12, a 12-base pair linker insertion into the 3' end of the pol gene, causes a temperature-sensitive phenotype. This mutant virus is temperature-sensitive for IN protein function as well as viral replication.
Schwartzberg
P L
PL
Department of Biochemistry, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Roth
M J
MJ
Tanese
N
N
Goff
S P
SP
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D015752:Genes, env; D015750:Genes, gag; D015751:Genes, pol; D019426:Integrases; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000201:enzymology / Q000235:genetics*; D016296:Mutagenesis; D015345:Oligonucleotide Probes; D010641:Phenotype; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012191:Retroviridae Proteins / Q000235:genetics* / Q000302:isolation & purification; D013696:Temperature; D014771:Virion / Q000201:enzymology / Q000235:genetics
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D012191:Retroviridae Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases