title: Two 3',5'-cyclic-adenosine monophosphate response elements in the promoter region of the human gastric inhibitory polypeptide gene.
abstract: Transfection of chimeric chloramphenicol acetyltransferase plasmids containing various deletions of the human gastric inhibitory polypeptide (GIP) promoter into hamster insulinoma (HIT T15) cells indicated that the region between -180 and +14 is sufficient for basal promoter activity. Two CRE-BP1 binding sites were identified in this promoter region by DNase I footprinting with the bacterially expressed cAMP response element (CRE) binding protein, CRE-BP1. Mutation analyses showed that these two CREs are required for the basal promoter activity, and furthermore that one site, at nucleotide-158, contributed mainly to the cAMP inducibility of the GIP promoter in HIT T15 cells. Interestingly, the GIP promoter activity was repressed by the c-jun proto-oncogene product, possibly through the CREs.
authors.affiliation: Department of Medicine, Kyoto University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Someya
authors.forename: N
authors.initials: N
authors.lastname: Inagaki
authors.forename: T
authors.initials: T
authors.lastname: Maekawa
authors.forename: Y
authors.initials: Y
authors.lastname: Seino
authors.forename: S
authors.initials: S
authors.lastname: Ishii
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D006224:Cricetinae; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D004247:DNA; D005749:Gastric Inhibitory Polypeptide / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007340:Insulinoma / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D017384:Sequence Deletion; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun; D005749:Gastric Inhibitory Polypeptide; D004247:DNA