Dihydroxyacetone test as a substitute for the dansyl chloride test.
The hazards of the dansyl chloride test are not negligible. We introduce the dihydroxyacetone test as a safe substitute. Several advantages are highlighted including easy and reproducible measurements of the modifications in skin color by chromometry in the L* a* b* (luminance, hue, chroma) mode. The color variations in time as well as the fade-out of DHA-induced pigmentation by some cleansing skin care products are revealed 'in vivo' as well as 'ex vivo' on D-Squame collections of stratum corneum.
Piérard
G E
GE
Department of Dermatopathology, University of Liège, Belgium.
Piérard-Franchimont
C
C
D000328:Adult; D003116:Color; D003124:Colorimetry; D003619:Dansyl Compounds / Q000494:pharmacology; D003879:Dermatologic Agents / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004098:Dihydroxyacetone / Q000494:pharmacology; D005453:Fluorescence; D006801:Humans; D008297:Male; D008543:Melanins / Q000378:metabolism*; D008875:Middle Aged; D010328:Patch Tests; D012867:Skin / Q000378:metabolism*; D012880:Skin Pigmentation / Q000187:drug effects / Q000502:physiology*
D003619:Dansyl Compounds; D003879:Dermatologic Agents; D003902:Detergents; D008543:Melanins; D004098:Dihydroxyacetone; C029038:dansyl chloride