Gene II product of an aphid-nontransmissible isolate of cauliflower mosaic virus expressed in a baculovirus system possesses aphid transmission factor activity.
A new baculovirus expression system was used to express the gene II which encodes the 18-kDa aphid transmission factor (ATF) protein of two isolates of cauliflower mosaic virus (CaMV), the aphid-transmissible (AT+) Cabb B-JI and the aphid-nontransmissible (AT-) CM1841 isolates. ATF activity was shown to be associated with the baculovirus expression product of CaMV gene II from both the AT+ and the AT- isolates. No differences could be detected in the stability of the P18s from AT+ and AT- isolates expressed in the baculovirus system. These results indicate that the AT- phenotype of CM1841 isolate might not be due to a defective P18 but to an insufficient concentration of it in infected plant tissue.
Blanc
S
S
Department of Virus Research, John Innes Institute, Norwich, United Kingdom.
Cerutti
M
M
Chaabihi
H
H
Louis
C
C
Devauchelle
G
G
Hull
R
R
D000818:Animals; D001042:Aphids; D016367:Baculoviridae / Q000235:genetics*; D001937:Brassica; D002460:Cell Line; D005814:Genes, Viral; D007313:Insecta; D009029:Mosaic Viruses / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D010940:Plant Proteins; D014764:Viral Proteins