title: Structure of a sarcoplasmic calcium-binding protein from amphioxus refined at 2.4 A resolution.
abstract: The three-dimensional structure of a sarcoplasmic Ca(2+)-binding protein from the protochordate amphioxus has been determined at 2.4 A resolution using multiple-isomorphous-replacement techniques. The refined model includes all 185 residues, three calcium ions, and one water molecule. The final crystallographic R-factor is 0.199. Bond lengths and bond angles in the molecules have root-mean-square deviations from ideal values of 0.015 A and 2.8 degrees, respectively. The overall structure is highly compact and globular with a predominantly hydrophobic core, unlike the extended dumbbell-shaped structures of calmodulin or troponin C. There are four distinct domains with the typical helix-loop-helix Ca(2+)-binding motif (EF hand). The conformation of the pair of EF hands in the N-terminal half of the protein is unusual due to the presence of an aspartate residue in the twelfth position of the first Ca(2+)-binding loop, rather than the usual glutamate. The C-terminal half of the molecule contains one Ca(2+)-binding domain with a novel helix-loop-helix conformation and one Ca(2+)-binding domain that is no longer functional because of amino acid changes. The overall structure is quite similar to a sarcoplasmic Ca(2+)-binding protein from sandworm, although there is only about 12% amino acid sequence identity between them. The similarity of the structures of these two proteins suggests that all sarcoplasmic Ca(2+)-binding proteins will have the same general conformation, even though there is very little conservation of primary structure among the proteins from various species.
authors.affiliation: Department of Pathology, University of Alabama, Birmingham 35294.
authors.forename: W J
authors.initials: WJ
authors.lastname: Cook
authors.forename: L C
authors.initials: LC
authors.lastname: Jeffrey
authors.forename: J A
authors.initials: JA
authors.lastname: Cox
authors.forename: S
authors.initials: S
authors.lastname: Vijay-Kumar
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D002816:Chordata, Nonvertebrate; D005583:Fourier Analysis; D010100:Oxygen / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012519:Sarcoplasmic Reticulum / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D002135:Calcium-Binding Proteins; D010100:Oxygen; D002118:Calcium