Colchicine alters lactate utilization in isolated hepatocytes of rats treated with CCl4 and ethanol.
Lactic acidosis has been described in patients with liver disease. Hyperlactacidaemia results from an imbalance in lactate production versus lactate utilization. It is estimated that the liver utilizes approximately 30 percent of the total lactate produced in the body under basal conditions, primarily by gluconeogenesis. The gluconeogenesis from lactate 10 mM and lactacidaemia were determined in order to investigate the effects of CCl4+ethanol administration in liver injury and, the possible effect of colchicine in our experimental fibrosis model. The tests were determined after 15, 30 or 45 days of treatment. The results indicate that the gluconeogenesis was significantly inhibited in both CCl4+ethanol groups and CCl4+ethanol+colchicine groups. By contrast, the lactacidaemia levels were much higher in the CCl4+ethanol groups than the colchicine groups. Summarising, we have documented that hyperlactacidaemia is due to the inhibition of lactate utilization by the isolated hepatocytes in experimental cirrhosis, and that the improvement in lactacidaemia caused by colchicine is not primarily due to an increase in hepatic lactate utilization.
Meniño
M J
MJ
Department of Physiology, Faculty of Biology, University of Santiago de Compostela, Galicia, Spain.
Cutrín
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C
Vieira
R
R
Barrio
E
E
Parafita
M A
MA
D000435:Alcoholic Intoxication / Q000378:metabolism*; D000818:Animals; D002251:Carbon Tetrachloride / Q000008:administration & dosage; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology*; D000431:Ethanol / Q000008:administration & dosage; D005943:Gluconeogenesis / Q000187:drug effects; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D007773:Lactates; D019344:Lactic Acid; D000431:Ethanol; D002251:Carbon Tetrachloride; D003078:Colchicine