title: Altered expression of the cyclin D1 and retinoblastoma genes in human esophageal cancer.
abstract: We have examined DNA from four human esophageal carcinoma cell lines and 50 primary esophageal carcinomas obtained from China, Italy, and France for amplification of the cyclin D1 gene. We also examined 36 of these 50 carcinomas for expression of the cyclin D1 and retinoblastoma (RB) proteins by immunohistochemistry. We found a 3- to 10-fold amplification of the cyclin D1 gene in 16 of the 50 (32%) tumors and in two of the four cell lines. Cyclin D1 protein was overexpressed in 12 of 13 tumors and the two cell lines that showed gene amplification when compared to normal controls. Studies on RB protein expression indicated that 6 of the 36 (17%) tumor samples examined and one cell line did not show detectable expression of this protein. The tumors and cell lines that had cyclin D1 gene amplification and overexpression exhibited normal levels of expression of RB protein. By contrast, the tumors and cell line that did not appear to express the RB protein did not show amplification of the cyclin D1 gene and expressed only low levels of the cyclin D1 protein (P = 0.03). These results suggest that the inhibitory effect of RB on cell cycle progression can be abrogated during tumor development either by loss of expression of the RB gene or by increased expression of the cyclin D1 gene.
authors.affiliation: Comprehensive Cancer Center, Columbia University, College of Physicians and Surgeons, New York, NY 10032.
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhang
authors.forename: S M
authors.initials: SM
authors.lastname: Kahn
authors.forename: M C
authors.initials: MC
authors.lastname: Hollstein
authors.forename: R M
authors.initials: RM
authors.lastname: Santella
authors.forename: S H
authors.initials: SH
authors.lastname: Lu
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
authors.forename: R
authors.initials: R
authors.lastname: Montesano
authors.forename: I B
authors.initials: IB
authors.lastname: Weinstein
mesh_terms: D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D019938:Cyclin D1; D016213:Cyclins / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004938:Esophageal Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D016161:Genes, Retinoblastoma; D006801:Humans; D007150:Immunohistochemistry; D008970:Molecular Weight; D015513:Oncogene Proteins / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D016160:Retinoblastoma Protein / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D014407:Tumor Cells, Cultured
chemical_list: D016213:Cyclins; D004273:DNA, Neoplasm; D015513:Oncogene Proteins; D016160:Retinoblastoma Protein; D019938:Cyclin D1