Establishment of a new human pre-B acute lymphoblastic leukemia cell line (KMO-90) with 1;19 translocation carrying p53 gene alterations.
A new human pre-B acute lymphoblastic leukemia cell line (KMO-90) was established from the bone marrow sample of a 12-year-old girl with acute lymphoblastic leukemia (ALL) carrying 1;19 chromosome translocation. KMO-90 cells expressed HLA-DR, CD10, CD19, and CD22 antigens. These cells had also cytoplasmic immunoglobulin lacking surface immunoglobulin, indicating that these had a pre-B phenotype. Chromosome analysis of this cell line showed 48, XX, +8, +19, t(1;19)(q23;p13). Southern blot analysis showed the same sized rearrangements of the E2A gene in KMO-90 cells as those in the original leukemic cells. By means of reverse transcriptase-polymerase chain reaction analysis, we detected E2A/PBX1 fusion transcripts in KMO-90 cells. KMO-90 is useful when studying the role of the 1;19 translocation in the etiology of pre-B ALL. Furthermore, we studied alterations of the p53 gene in this cell line by polymerase chain reaction, single-strand conformation polymorphism analysis. KMO-90 cells were identified to have a point mutation at codon 177 (CCC-->TCC) of the p53 gene, suggesting that alterations of the p53 gene may have an important role in the establishment of this cell line.
Sotomatsu
M
M
Department of Pediatrics, Gunma University School of Medicine, Maebashi, Japan.
Hayashi
Y
Y
Kawamura
M
M
Yugami
S
S
Shitara
T
T
D000954:Antigens, Surface / Q000032:analysis; D001483:Base Sequence; D015139:Blotting, Southern; D002648:Child; D002878:Chromosomes, Human, Pair 1 / Q000502:physiology*; D002888:Chromosomes, Human, Pair 19 / Q000502:physiology*; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000032:analysis; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology*; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded