Method for 3D volumetric analysis of intranuclear fluorescence distribution in confocal microscopy.
The introduction of the confocal laser scanning microscope makes it possible to acquire fluorescent specimens in 3D. We present basic image processing tools to enhance the data and to quantitate the morphology and topography of the intravolume elements. The tools were applied to the description of the spatial distribution of DNA replication sites in mammalian cell nuclei as an example.
Parazza
F
F
Equipe de Reconnaissance des Formes et Microscopie Quantitative, Laboratoire TIM3-IMAG, USR CNRS 690B, CERMO, Université Joseph Fourier, Grenoble, France.
Humbert
C
C
Usson
Y
Y
D000465:Algorithms; D016477:Artifacts; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D003199:Computer Systems; D004247:DNA / Q000648:ultrastructure; D004261:DNA Replication; D003628:Database Management Systems; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D008854:Microscopy, Electron / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D016196:S Phase / Q000235:genetics; D014463:Ultrasonography
D004247:DNA