A factor released by monocytes in the presence of dexamethasone stimulates neutrophil locomotion.
1. Steroid-treated monocyte supernatants cause a dramatic increase in the speed of locomotion of human neutrophils and a significant decrease in their adhesion to protein-coated glass. In contrast, control monocyte supernatants have a smaller effect on the speed of locomotion, but cause a large increase in their adhesiveness. 2. This supernatant activity was produced equally well in the presence or absence of serum after 24 h culture at 37 degrees C with 10(-6) M dexamethasone. 3. The effect of the steroid-treated monocyte supernatants on the speed of locomotion of human peripheral blood neutrophils was not altered by rabbit polyclonal antisera against lipocortins 1-6. 4. Rabbit anti-interleukin-8 antibody which blocked the effect of IL-8 on the speed of locomotion of neutrophils did not antagonize the locomotion stimulating action of steroid-treated monocyte supernatants. 5. The exocellular release of this factor(s) by human mononuclear leucocytes suggests that it may be an in vivo mediator of the anti-inflammatory effect of glucocorticoids.
Chettibi
S
S
Department of Cell Biology, University of Glasgow.
Lawrence
A J
AJ
Stevenson
R D
RD
D017302:Annexins / Q000737:chemistry / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D008858:Microscopy, Phase-Contrast; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*
D017302:Annexins; D007106:Immune Sera; D016209:Interleukin-8; D003907:Dexamethasone