Temperature-sensitive transforming mutants of the v-rel oncogene.
By making site-directed mutations in the avian retroviral oncogene v-rel, we created two temperature-sensitive (ts) transforming mutants; these changes were analogous to mutations previously shown to confer a ts function onto the Dorsal protein of Drosophila melanogaster. Chicken spleen cells infected with the ts v-rel mutants formed colonies in agar at 36.5 degrees C but not at 41.5 degrees C. In addition, spleen cells derived from the ts v-rel-transformed colonies could be propagated in liquid culture at 36.5 degrees C but rapidly senesced at 41.5 degrees C. Both mutant v-Rel proteins were also ts for DNA binding in vitro. These mutants may be valuable for identifying genes directly regulated by v-rel.
White
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Department of Biology, Boston University, Massachusetts 02215.
Gilmore
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002645:Chickens; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D020572:Oncogene Proteins v-rel; D009857:Oncogenes; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics
D009838:Oligodeoxyribonucleotides; D020572:Oncogene Proteins v-rel; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors