Expression in E. coli and purification of a chimeric p22-NS3 recombinant antigen of hepatitis C virus (HCV).
A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a starting point for the design of an HCV single all-encompassing antigen for a blood screening assay.
Osborne
S
S
R&D Diagnostic Division, SORIN Biomedica S.p.A., Saluggia, Italy.
Cecconato
E
E
Griva
S
S
Garetto
F
F
Calogero
R
R
Rosa
C
C
Bonelli
F
F
D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D016174:Hepacivirus / Q000737:chemistry / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000276:immunology*
D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins