title: Variable and constant regions in the C-terminus of vinculin and metavinculin. Cloning and expression of fragments in E. coli.
abstract: Metavinculin differs from vinculin in having an additional insert of 68 to 79 amino acids in length in the C-terminal half of the molecule. Cross-species comparison of metavinculin sequences from pig, man, chicken and frog reveals a division of the insert into two parts: the first variable and the second highly conserved. The longest insert, 79 amino acids, was found in Xenopus laevis. Three different C-terminal constructs of vinculin and metavinculin over-expressed in E. coli could be purified by column chromatography. Two-dimensional gel electrophoresis and peptide analysis revealed pI values between 8.35 and 10.25 for the recombinant proteins. Biochemical and structural features of the metavinculin-specific sequence and the conserved vinculin/metavinculin carboxy-terminus are discussed.
authors.affiliation: Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
authors.forename: P
authors.initials: P
authors.lastname: Strasser
authors.forename: M
authors.initials: M
authors.lastname: Gimona
authors.forename: M
authors.initials: M
authors.lastname: Herzog
authors.forename: B
authors.initials: B
authors.lastname: Geiger
authors.forename: J V
authors.initials: JV
authors.lastname: Small
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis / Q000737:chemistry*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D016596:Vinculin / Q000096:biosynthesis / Q000737:chemistry*
chemical_list: D030161:Avian Proteins; D009124:Muscle Proteins; D011994:Recombinant Proteins; C035689:VCL protein, Gallus gallus; C488955:VCL protein, human; D016596:Vinculin