title: A tetranucleotide repeat mouse minisatellite displaying substantial somatic instability during early preimplantation development.
abstract: The highly variable mouse minisatellite Hm-2 is located on chromosome 9 and consists of GGCA tetranucleotide repeats with alleles containing up to 5000 repeat units. This locus is unstable with a germline mutation rate to new length alleles of at least 3.6% per gamete. Hm-2 also shows substantial somatic instability, producing mutational mosaicism detectable in 20% of adult mice. Analysis of allele dosage in mice carrying somatic mutations, plus studies of mosaicism in mouse embryos and extraembryonic tissues, suggests that somatic mutant alleles preferentially arise during preimplantation development and particularly during the first two cell divisions after fertilization.
authors.affiliation: Department of Genetics, University of Leicester, England.
authors.forename: M
authors.initials: M
authors.lastname: Gibbs
authors.forename: A
authors.initials: A
authors.lastname: Collick
authors.forename: R G
authors.initials: RG
authors.lastname: Kelly
authors.forename: A J
authors.initials: AJ
authors.lastname: Jeffreys
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst; D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000235:genetics*; D005314:Embryonic and Fetal Development / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology / Q000235:genetics*; D008969:Molecular Sequence Data; D009030:Mosaicism / Q000235:genetics; D009154:Mutation; D009928:Organ Specificity; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004276:DNA, Satellite