Sequence-independent amplification and labeling of yeast artificial chromosomes for fluorescence in situ hybridization.
We have developed a method that allows reliable construction of high quality FISH probes from yeast artificial chromosomes (YACs) based on the separation of YACs by pulse-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can be used to localize YACs on metaphase chromosomes and also with high efficiency, in interphase nuclei.
Bohlander
S K
SK
Section of Hematology/Oncology, University of Chicago, Illinois 60637.
Espinosa
R
R
Fernald
A A
AA
Rowley
J D
JD
Le Beau
M M
MM
Díaz
M O
MO
D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D017931:DNA Primers; D015342:DNA Probes; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D015342:DNA Probes