title: Production of interleukin 6, leukemia inhibitory factor and granulocyte-macrophage colony stimulating factor by peripheral blood mononuclear cells in Fanconi's anemia.
abstract: The aim of this study was to test the in vitro cytokine production by peripheral blood mononuclear cells (PBMCs) in patients with Fanconi's anemia (FA). Phytohemagglutinin (PHA)-stimulated PBMCs from 21 patients with FA were studied for their ability to produce interleukin 6 (IL-6), leukemia inhibitory factor (LIF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Enzymatic immunoassay (EIA) was used for both IL-6 and LIF, while GM-CSF was evaluated in a highly sensitive biological assay provided by GM-CSF-dependent M-07e cells. A significant decrease of IL-6 was detected in 9 out of 11 FA patients compared with five normal donors, while similar amounts of LIF were produced from 21 FA patients and 21 healthy subjects. A drastic increase of active GM-CSF was documented in PHA-stimulated PBMC-conditioned medium in all 18 FA patients tested. Since IL-6 and GM-CSF play an important role in maintaining basal hemopoiesis, our results suggest that an abnormal cytokine network may be involved in the pathogenesis of FA pancytopenia.
authors.affiliation: G. Prodi Interdepartmental Center for Cancer Research, University of Bologna, Italy.
authors.forename: G P
authors.initials: GP
authors.lastname: Bagnara
authors.forename: L
authors.initials: L
authors.lastname: Bonsi
authors.forename: P
authors.initials: P
authors.lastname: Strippoli
authors.forename: U
authors.initials: U
authors.lastname: Ramenghi
authors.forename: F
authors.initials: F
authors.lastname: Timeus
authors.forename: F
authors.initials: F
authors.lastname: Bonifazi
authors.forename: M
authors.initials: M
authors.lastname: Bonafè
authors.forename: R
authors.initials: R
authors.lastname: Tonelli
authors.forename: G
authors.initials: G
authors.lastname: Bubola
authors.forename: M F
authors.initials: MF
authors.lastname: Brizzi
mesh_terms: D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D017077:Culture Media, Conditioned; D005199:Fanconi Anemia / Q000097:blood* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000097:blood; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000097:blood; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000097:blood; D053605:Leukemia Inhibitory Factor; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D017077:Culture Media, Conditioned; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D010835:Phytohemagglutinins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor