Modulation of the ATP induced [Ca2+]c increase in AS-30D hepatoma cells.
1. The regulation of the increase in the cytosolic calcium concentration ([Ca2+]c) induced by extracellular ATP in AS-30D hepatoma cells was studied. 2. Homologous desensitization involving the refilling of intracellular calcium pools and the participation of protein kinase C was found. 3. Isoproterenol, forskolin and dibutyryl-cyclic AMP also induced an increase in [Ca2+]c. 4. Interestingly, synergism was found for isoproterenol or forskolin and ATP. 5. The results suggest that there are two pathways for mobilizing [Ca2+]c in AS-30D hepatoma cells; one is activated by ATP receptors and the other by cyclic AMP.
Torres-Márquez
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ME
Departamento de Bioquímica, Instituto Nacional de Cardiología, México, D.F.
Mejia
S
S
Moreno-Sánchez
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R
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004357:Drug Synergism; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D010703:Phorbol Esters; D005576:Colforsin; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D007545:Isoproterenol; D002118:Calcium