title: Development of androgenetic mouse embryos produced by in vitro fertilization of enucleated oocytes.
abstract: Enucleated mouse oocytes were successfully fertilized in vitro, and the resultant androgenetic eggs developed to the blastocyst stage. The proportion of enucleated oocytes fertilized in vitro was high (87-99%) at sperm concentrations ranging from 10-100 x 10(4)/ml. At high sperm concentrations (100-1,000 x 10(4)), 35-45% of the fertilized eggs resulted in heterozygous bispermic androgenones. The proportion of hemizygous haploid and heterozygous diploid androgenones developing to blastocysts was 11% and 43%, respectively. Hemizygous diploidization, however, showed no positive effect on development. These results clearly show that the procedure reported here is efficient and reliable for the production of androgenetic eggs.
authors.affiliation: NODAI Research Institute, Tokyo University of Agriculture, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kono
authors.forename: Y
authors.initials: Y
authors.lastname: Sotomaru
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Nakahara
mesh_terms: D000818:Animals; D004171:Diploidy; D005314:Embryonic and Fetal Development; D005307:Fertilization in Vitro; D006238:Haploidy; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000648:ultrastructure*