Regulatory circuit for responses of nitrogen catabolic gene expression to the GLN3 and DAL80 proteins and nitrogen catabolite repression in Saccharomyces cerevisiae.
We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1 and PUT2, also required a functional GLN3 protein. In addition, GLN3 protein was required for expression of the DAL1, DAL2, DAL7, GDH1, and GDH2 genes. The UGA1, CAN1, GAP1, and DAL4 genes markedly increased their expression when the DAL80 locus, encoding a negative regulatory element, was disrupted. Expression of the GDH1, PUT1, PUT2, and PUT4 genes also responded to DAL80 disruption, but much more modestly. Expression of GLN1 and GDH2 exhibited parallel responses to the provision of asparagine and glutamine as nitrogen sources but did not follow the regulatory responses noted above for the nitrogen catabolic genes such as DAL5. Steady-state mRNA levels of both genes did not significantly decrease when glutamine was provided as nitrogen source but were lowered by the provision of asparagine. They also did not respond to disruption of DAL80.
Daugherty
J R
JR
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Rai
R
R
el Berry
H M
HM
Cooper
T G
TG
D000481:Allantoin / Q000494:pharmacology; D001216:Asparagine / Q000494:pharmacology; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D050980:GATA Transcription Factors; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D005973:Glutamine / Q000494:pharmacology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D011392:Proline / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
C071662:DAL80 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D050980:GATA Transcription Factors; C071664:GLN3 protein, S cerevisiae; D012333:RNA, Messenger; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005973:Glutamine; D000481:Allantoin; D005680:gamma-Aminobutyric Acid; D001216:Asparagine; D011392:Proline; D009584:Nitrogen