Different target-site specificities of the hairpin ribozyme in cis and trans cleavages.
The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.
Fujitani
K
K
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Sasaki-Tozawa
N
N
Kikuchi
Y
Y
D001483:Base Sequence; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry; D013379:Substrate Specificity
D016337:RNA, Catalytic; D012367:RNA, Viral