[Evaluation of the combined action of muscarinic receptor antagonists and beta receptor agonists on reactivity of basophils isolated from blood of patients with atopic bronchial asthma].
The study aimed at evaluating effects of selective M1 receptors antagonist-pirenzepine-and selected beta-receptor-agonists-orciprenaline or salbutamol, given alone or in combination, on histamine release from basophils isolated from patients with atopic asthma. Histamine concentration in the cells was assayed with spectrofluorimetric technique described by Shor and modified by Scov and Norn, using anti-IgE and metacholine as liberators. It was showed, that pirenzepine inhibits histamine release caused by both anti-IgE and metacholine. In the latter case this inhibitory effect was more significant. Beta-sympathicomimetics acted conversely, significantly inhibiting immunological histamine release. This effect was weaker, if metacholine was used. Combination of these agents increased protective effect of pirenzepine on histamine release induced by metacholine.
Michalska
I
I
Kliniki Alergologii AM, Białymstoku.
Siergiejko
Z
Z
Hofman
J
J
D000328:Adult; D000420:Albuterol / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D001491:Basophils / Q000187:drug effects*; D004359:Drug Therapy, Combination; D006632:Histamine / Q000032:analysis; D006801:Humans; D009921:Metaproterenol / Q000627:therapeutic use*; D008875:Middle Aged; D010890:Pirenzepine / Q000627:therapeutic use*
D010890:Pirenzepine; D009921:Metaproterenol; D006632:Histamine; D000420:Albuterol