In vitro transcription and replication of the mumps virus genome.
By the use of lysolecithin-permealized extracts from mumps virus-infected HeLa cells, we have developed an in vitro system, which not only directed the synthesis of mumps virus mRNAs but also supported replication of the genomic RNA. Furthermore, upon transcription of the P gene, both faithful and edited copies of the P gene were detected by RNase mapping with a riboprobe. Thus this system seems to promote biochemical analyses of underlying mechanisms operative in mumps virus gene expression and replication, including RNA editing.
Takeuchi
K
K
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Tanabayashi
K
K
Okazaki
K
K
Hiahiyama
M
M
Yamada
A
A
D002474:Cell-Free System; D016679:Genome, Viral; D006367:HeLa Cells; D006801:Humans; D009109:Mumps virus / Q000235:genetics* / Q000502:physiology; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic; D014779:Virus Replication
D012367:RNA, Viral