Molecular cloning of a mouse extracellular signal regulated kinase (erk-1).
A complementary DNA has been cloned and sequenced for the mouse extracellular signal regulated kinase (erk-1). The nucleotide sequence of the 1.7 kb mouse erk-1 cDNA clone (merk-1-34) is 93.5% identical to the published rat DNA sequence (Boulton et al. (1990) Science 249, 64-67). The mouse clone, as with all other reported sequences, did not contain an in-frame translation initiation codon and, therefore, does not appear to be full-length. Attempts to generate cDNA sequence upstream of the 5' end of merk-1-34 using thermostable reverse transcriptase and chemical melting of the mRNA (methylmercury hydroxide) were unsuccessful.
Tanner
B
B
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110.
Mueckler
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D051379:Mice; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000737:chemistry*
D011494:Protein Kinases; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases