Serum albumin concentration-related Health Care Financing Administration quality assurance criterion is method-dependent: revision is necessary.
The objective of this study was to examine quantitative differences between the two commonly used methods for determining serum albumin concentration, bromcresol green (BCG) and bromcresol purple (BCP), in normal subjects and in 235 unselected dialysis patients in view of recently established Health Care Financing Administration (HCFA) quality assurance review criteria. The mean of normal results by the BCG method was 4.4 g/dL, and 97.5% of values were 3.8 g/dL or higher. The mean of normal results by the BCP method was 3.9 g/dL, and 97.5% of values were 3.3 g/dL or higher. Serum albumin concentrations in samples from the dialysis patients had respectively lower mean values by both methods. For the BCG method, the mean was 3.8 g/dL, and 82% of values were 3.5 g/dL or higher; for the BCP method, the mean was 3.3 g/dL, and 82% of values were 3.0 g/dL or higher. Likewise, for the reference immunonephelometric procedure, the mean value for the dialysis patients was 3.3 g/dL, and 82% of values were 3.0 g/dL or higher. For the samples from the dialysis patients, in comparison with the immunonephelometric method, the BCG method exhibited both constant (intercept = 9.3 g/L) and proportional error (slope = 0.87). The mean albumin value for the BCG method was 3.8 g/dL, 15% higher. In contrast, the BCP method compared closely with the reference method: slope = 1.00, intercept = 0.8 g/L, mean x = 3.3 g/dL, mean y = 3.3 g/dL. The HCFA quality assurance criteria are valid only for the BCG method.(ABSTRACT TRUNCATED AT 250 WORDS)
Blagg
C R
CR
Northwest Kidney Centers, Laboratory of Pathology of Seattle, Inc., WA 98122.
Liedtke
R J
RJ
Batjer
J D
JD
Racoosin
B
B
Sawyer
T K
TK
Wick
M J
MJ
Lawson
L
L
Wilkens
K
K
D001961:Bromcresol Green; D001962:Bromcresol Purple; D002138:Calibration; D014487:Centers for Medicare and Medicaid Services, U.S.; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D009391:Nephelometry and Turbidimetry / Q000592:standards; D010530:Peritoneal Dialysis / Q000592:standards*; D011785:Quality Assurance, Health Care / Q000592:standards*; D012016:Reference Values; D006435:Renal Dialysis / Q000592:standards*; D012709:Serum Albumin / Q000032:analysis*; D014481:United States
D012709:Serum Albumin; D001962:Bromcresol Purple; D001961:Bromcresol Green