Biosynthesis and in vivo localization of the decapentaplegic-Vg-related protein, DVR-6 (bone morphogenetic protein-6).
DVR-6 (BMP-6 or Vgr-1) is a member of the TGF-beta superfamily of polypeptide signaling molecules. In situ hybridization studies have previously shown that DVR-6 RNA is expressed in a variety of cell types in the mouse embryo, but no information has been available on protein localization and biosynthesis. We have produced a polyclonal antibody to the proregion of DVR-6 and used it to localize the protein in whole mount and sectioned embryonic, newborn, and adult mouse tissues. DVR-6 protein is expressed in the mouse nervous system beginning at 9.5 days postcoitum (d.p.c.) and continues through adulthood. A variety of epithelial tissues also produce DVR-6 protein, including the suprabasal layer of the skin, bronchiolar epithelium, and the cornea. Additionally, a stably transfected cell line, BMGE+H/D6c4, is used to study the biosynthesis of DVR-6 protein and evidence is presented for translational regulation of DVR-6 expression.
Wall
N A
NA
Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee 37232.
Blessing
M
M
Wright
C V
CV
Hogan
B L
BL
D000818:Animals; D019485:Bone Morphogenetic Proteins; D002417:Cattle; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D006133:Growth Substances / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008321:Mammary Glands, Animal / Q000502:physiology; D051379:Mice; D009420:Nervous System / Q000166:cytology / Q000196:embryology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis; D014158:Transcription, Genetic; D014162:Transfection
D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase