title: Crystallization and preliminary X-ray diffraction studies of Bacillus stearothermophilus farnesyl diphosphate synthase expressed in Escherichia coli.
abstract: Thermostable farnesyl diphosphate synthase (EC 2.5.1.10) from Bacillus stearothermophilus, which was overexpressed in Escherichia coli, has been crystallized by the vapor-diffusion procedure. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystals diffracted X-rays to about 3 A resolution. The diffraction pattern indicated that the space group is I4(1)22 with unit-cell dimensions of a = b = 114 A and c = 247 A. It is thought that the asymmetric unit comprises two or three molecules of farnesyl diphosphate synthase.
authors.affiliation: Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakane
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: S
authors.initials: S
authors.lastname: Obata
authors.forename: M
authors.initials: M
authors.lastname: Osabe
authors.forename: A
authors.initials: A
authors.lastname: Takeshita
authors.forename: T
authors.initials: T
authors.lastname: Nishino
authors.forename: K
authors.initials: K
authors.lastname: Ogura
authors.forename: K
authors.initials: K
authors.lastname: Miki
mesh_terms: D019883:Alkyl and Aryl Transferases; D003001:Cloning, Molecular; D003460:Crystallization; D004926:Escherichia coli; D001411:Geobacillus stearothermophilus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D051228:Geranyltranstransferase; D014166:Transferases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051228:Geranyltranstransferase