Comparison of the effects of insulin-like growth factors-I and -II on the human osteosarcoma cell line OHS-4.
The effects of insulin-like growth factor-I (IGF-I) and IGF-II on the human osteoblast cell-line OHS-4 were investigated. Both IGF-I and IGF-II stimulated cell proliferation at nanomolar concentrations and alkaline phosphatase activity was decreased in a dose-dependent manner with either IGF-I or IGF-II. The production of the bone-specific protein osteocalcin was not influenced by either IGF-I or IGF-II. However, they acted synergistically with 1,25-dihydroxy-vitamin D3 at concentrations ranging from 10 to 100 nmol/l. Neither IGF-I nor IGF-II had an effect on either the basal or the parathyroid hormone-stimulated level of adenylate cyclase activity, and likewise they had no effect on phosphodiesterase activity. Binding and cross-linking experiments confirmed the presence of both type-I and type-II IGF receptors on the OHS-4 cells. The present study shows that IGF-I and IGF-II have similar effects on the parameters studied in these osteoblastic cells. They influenced both proliferation and differentiation markers.
Fournier
B
B
Pharmaceuticals Research Laboratories, CIBA-GEIGY Ltd, Basel, Switzerland.
Ferralli
J M
JM
Price
P A
PA
Schlaeppi
J M
JM
D000262:Adenylyl Cyclases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects / Q000201:enzymology; D012516:Osteosarcoma / Q000188:drug therapy* / Q000201:enzymology; D010281:Parathyroid Hormone / Q000494:pharmacology; D017451:Receptors, Somatomedin / Q000378:metabolism
D010281:Parathyroid Hormone; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D000469:Alkaline Phosphatase; D000262:Adenylyl Cyclases