title: Physical map of the extremely thermophilic bacterium Thermus thermophilus HB27 chromosome.
abstract: The physical map of the chromosome of Thermus thermophilus HB27 was constructed using three restriction enzymes; EcoRI, SspI and MunI by applying pulsed-field gel electrophoresis techniques. Although the genome size of 1.82 Mb was almost the same as that (1.74 Mb) reported for T. thermophilus HB8 [Borges, K.M., and P.L. Bergquist. (1993) J. Bacteriol. 175, 103-110], the MunI cleavage maps were different. A 240 kb plasmid was detected in HB27, and its physical map was also constructed. In addition, several genes were located on the chromosomal physical map.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tabata
authors.forename: T
authors.initials: T
authors.lastname: Kosuge
authors.forename: T
authors.initials: T
authors.lastname: Nakahara
authors.forename: T
authors.initials: T
authors.lastname: Hoshino
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D010957:Plasmids; D016963:Thermus thermophilus / Q000235:genetics*
chemical_list: D004262:DNA Restriction Enzymes