A study of colchicine tubulin complex by donor quenching of fluorescence energy transfer.
The utility of collisional quenching of energy donors in fluorescence energy transfer is described. In multi-donor single acceptor systems, which contain different classes of donors (as distinguished by their accessibility towards a collisional quencher), donor quenching may be used to assess the fraction of energy transfer from each class of donor. The tubulin-colchicine complex was used as a donor-acceptor system to show that two inaccessible tryptophans are at or near the colchicine binding site.
Bhattacharyya
A
A
Department of Biophysics, Bose Institute, Calcutta, India.
Bhattacharyya
B
B
Roy
S
S
D020106:Acrylamide; D000178:Acrylamides / Q000737:chemistry; D000426:Alcohol Dehydrogenase / Q000737:chemistry; D000818:Animals; D003078:Colchicine / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004735:Energy Transfer; D006736:Horses; D008099:Liver / Q000201:enzymology; D009243:NAD / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000737:chemistry; D014404:Tubulin / Q000737:chemistry*
D000178:Acrylamides; D014404:Tubulin; D009243:NAD; D020106:Acrylamide; D014364:Tryptophan; D000426:Alcohol Dehydrogenase; D003078:Colchicine; D004121:Dimethyl Sulfoxide