[Detection of minimal residual clone after sex-mismatched bone marrow transplantation by fluorescent in situ hybridization].
In our previous paper we reported that fluorescent in situ hybridization (FISH) using DYZ1 and DXZ1 was a highly reproducible technique and cellular chimerism consisting of cells of male and female origins could be detected in the order of 0.1%. In the present study FISH was applied to detect minimal residual clones (MRC) in 10 patients with a variety of leukemias who received sex-mismatched bone marrow transplantation (BMT). In 9 patients who had no signs of recurrence, serial FISHs performed on each patient revealed the presence of 0.1 to 3.2% recipient residual clone. On the other hand, in the remaining one patient 10.6% and 16.9% of MRC were found by FISH performed 6 and 10 months after BMT. Immediately thereafter, this patient was diagnosed as in relapse by bone marrow examination.
Ariyama
T
T
Department of Hygiene, Kyoto Prefectural University of Medicine.
Inazawa
J
J
Akiyama
Y
Y
Yoshida
Y
Y
Okuma
M
M
Nagai
K
K
Horiuchi
A
A
Abe
T
T
D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology / Q000628:therapy; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000276:immunology; D002648:Child; D002999:Clone Cells; D005260:Female; D006085:Graft Survival; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000276:immunology; D006648:Histocompatibility; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000276:immunology / Q000628:therapy; D008297:Male; D011379:Prognosis; D012730:Sex Chromosomes