Inhibition of growth of Mycobacterium avium in murine and human mononuclear phagocytes by migration inhibitory factor.
Infections caused by Mycobacterium avium, the most common form of diseminated bacterial disease in AIDS patients, are difficult to treat because of their resistance to many antimycobacterial drugs. The results of the present study show that recombinant migration inhibitory factor, a 12-kDa molecule recently isolated by COS-1 cell expression screening of cDNA from a human T-cell hybridoma, has potent inhibitory activity on the growth of a panel of clinical isolates of M. avium within both bone-marrow-derived murine macrophages and cultured human blood monocytes. These cells cultured in recombinant migration inhibitory factor exhibit various signs of activation, including cell division, morphological changes such as evidence of substantial phagolysosomal fusion, and enhanced secretion of tumor necrosis factor.
Orme
I M
IM
Department of Microbiology, Colorado State University, Fort Collins 80523.
Furney
S K
SK
Skinner
P S
PS
Roberts
A D
AD
Brennan
P J
PJ
Russell
D G
DG
Shiratsuchi
H
H
Ellner
J J
JJ
Weiser
W Y
WY
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008262:Macrophage Activation / Q000187:drug effects; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D009164:Mycobacterium Infections / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D009162:Mycobacterium avium / Q000254:growth & development*; D010587:Phagocytosis / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008263:Macrophage Migration-Inhibitory Factors; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha