title: H1 receptor mediates inositol phosphates response to histamine in gastric smooth muscle of guinea pigs.
abstract: The effect of histamine on [3H]-inositol phosphates (IPs) formation was investigated with [3H]-inositol-labeled gastric smooth-muscle cells in guinea pigs. Histamine (10(-5) M) increased the formation of [3H]-IPs in the muscle cells. The increase was significantly inhibited by pyrilamine (10(-5) M) but not by cimetidine (10(-5) M). The contractile response to histamine was also completely inhibited by pyrilamine but not by cimetidine. Phorbol ester 12-myristate 13-acetate (10 microM) significantly inhibited histamine-stimulated [3H]-IPs formation by 56%, whereas forskolin (10 microM) decreased it by 18%. This study demonstrates that the response of [3H]-IPs formation and contraction to histamine is mediated through H1 receptor, and the formation of [3H]-IPs is negatively regulated by protein kinase C in gastric smooth muscle of guinea pigs.
authors.affiliation: Dept. of Physiology, Catholic University Medical College, Seoul, South Korea.
authors.forename: S S
authors.initials: SS
authors.lastname: Sim
authors.forename: Y H
authors.initials: YH
authors.lastname: Jo
authors.forename: S J
authors.initials: SJ
authors.lastname: Hahn
authors.forename: S H
authors.initials: SH
authors.lastname: Yoon
authors.forename: D J
authors.initials: DJ
authors.lastname: Rhie
authors.forename: M S
authors.initials: MS
authors.lastname: Kim
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000096:biosynthesis*; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D011494:Protein Kinases / Q000502:physiology; D011969:Receptors, Histamine H1 / Q000502:physiology*; D013270:Stomach / Q000502:physiology*
chemical_list: D007295:Inositol Phosphates; D011969:Receptors, Histamine H1; D006632:Histamine; D011494:Protein Kinases