A taxonomic review of the genus Microbispora by analysis of ribosomal protein AT-L30.
We analyzed the ribosomal AT-L30 proteins from 13 type strains of species belonging to the genera Microbispora and Actinomadura. The electrophoretic mobilities of the AT-L30 preparations from Microbispora strains, as determined by two-dimensional polyacrylamide gel electrophoresis, revealed that the members of the genus Microbispora are phylogenetically homogeneous. The results of partial amino acid sequencing of AT-L30 preparations from several representative Microbispora strains supported the separation of the genus Microbispora from other related genera. The amino acid sequences of the AT-L30 proteins from strains of species belonging to the genus Actinomadura sensu stricto displayed a diversity that exemplified the low levels of amino acid sequence homology within the genus. This diversity was considered to be a characteristic typical of the genus Actinomadura.
Ochi
K
K
National Food Research Institute, Ibaraki, Japan.
Haraguchi
K
K
Miyadoh
S
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D008848:Micromonosporaceae / Q000737:chemistry* / Q000145:classification*; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000032:analysis* / Q000235:genetics; D013045:Species Specificity
D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C035201:ribosomal protein L30