Post-capillary Immobilon-P membrane fraction collection for capillary electrophoresis.
A simple, cost-effective and efficient method of continuous fraction collection for capillary electrophoresis is described. The method employs a membrane, such as polyvinylidene difluoride, onto which the separated components from a capillary electrophoresis capillary are directly collected to overcome the problem of analyte dilution and to facilitate the fraction collection process. Model proteins are separated and collected onto a polyvinylidene difluoride membrane.
Cheng
Y F
YF
Millipore Corporation, Waters Chromatography Division, Milford, MA 01757.
Fuchs
M
M
Carson
W
W
D000818:Animals; D001709:Biotechnology; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D007782:Lactoglobulins / Q000302:isolation & purification; D008567:Membranes, Artificial; D009211:Myoglobin / Q000302:isolation & purification; D011145:Polyvinyls; D005465:Fluorocarbon Polymers
D007782:Lactoglobulins; D008567:Membranes, Artificial; D009211:Myoglobin; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers