title: The incidence of p53 mutations increases with progression of head and neck cancer.
abstract: To establish a genetic model of the progression of head and neck squamous carcinoma we have defined the incidence and timing of p53 mutations in this type of cancer. We sequenced the conserved regions of the p53 gene in 102 head and neck squamous carcinoma lesions. These included 65 primary invasive carcinomas and 37 noninvasive archival specimens consisting of 13 severe dysplasias and 24 carcinoma in situ lesions. The incidence of p53 mutations in noninvasive lesions was 19% (7/37) and increased to 43% (28/65) in invasive carcinomas. These data suggest that p53 mutations can precede invasion in primary head and neck cancer. Furthermore, the spectrum of codon hotspots is similar to that seen in squamous carcinoma of the lung and 64% of mutations are at G nucleotides, implicating carcinogens from tobacco smoke in the etiology of head and neck squamous carcinoma.
authors.affiliation: Department of Oral Surgery, Johns Hopkins University, Baltimore, Maryland.
authors.forename: J O
authors.initials: JO
authors.lastname: Boyle
authors.forename: J
authors.initials: J
authors.lastname: Hakim
authors.forename: W
authors.initials: W
authors.lastname: Koch
authors.forename: P
authors.initials: P
authors.lastname: van der Riet
authors.forename: R H
authors.initials: RH
authors.lastname: Hruban
authors.forename: R A
authors.initials: RA
authors.lastname: Roa
authors.forename: R
authors.initials: R
authors.lastname: Correo
authors.forename: Y J
authors.initials: YJ
authors.lastname: Eby
authors.forename: J M
authors.initials: JM
authors.lastname: Ruppert
authors.forename: D
authors.initials: D
authors.lastname: Sidransky
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology*; D002278:Carcinoma in Situ / Q000235:genetics / Q000473:pathology; D003001:Cloning, Molecular; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000302:isolation & purification; D005091:Exons; D005260:Female; D016158:Genes, p53; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D009361:Neoplasm Invasiveness; D016133:Polymerase Chain Reaction; D012907:Smoking
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm