Thiol and disulfide determination by free zone capillary electrophoresis.
A rapid, sensitive and simple method for the determination of reduced and oxidized glutathione, cysteine, cystine, cysteamine, cystamine and their respective mixed disulfides is described. The compounds were separated and identified in a single step by capillary zone electrophoresis. The method was used to follow the thiol-disulfide interconversion and to measure glutathione levels in lens extracts.
Cappiello
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Dipartimento di Fisiologia e Biochimica, Università di Pisa, Italy.
Del Corso
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Camici
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Mura
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D000818:Animals; D002417:Cattle; D003538:Cystamine / Q000032:analysis; D003543:Cysteamine / Q000032:analysis; D003545:Cysteine / Q000032:analysis; D003553:Cystine / Q000032:analysis; D004220:Disulfides / Q000032:analysis*; D004229:Dithiothreitol / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D005978:Glutathione / Q000031:analogs & derivatives / Q000032:analysis; D019803:Glutathione Disulfide; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*
D004220:Disulfides; D013438:Sulfhydryl Compounds; D003553:Cystine; D003543:Cysteamine; D005978:Glutathione; D003545:Cysteine; D003538:Cystamine; D004229:Dithiothreitol; D019803:Glutathione Disulfide