Inhibition of epidermal growth factor-stimulated hepatocytes proliferation by autologous sinusoidal mononuclear cells in rat liver.
Mononuclear cells isolated from sinusoid of rat liver by collagenase perfusion method showed a strong inhibitory effect on epidermal growth factor-stimulated proliferation of cocultured autologous hepatocytes, while mononuclear cells from peripheral blood and spleen did not. This inhibitory effect depended on the effector/target ratio. In a single-color flow cytometric analysis, about 25% of sinusoidal mononuclear cells consisted of asialo GM1+ natural killer cells, and there were relatively small numbers of CD3+ T cells and a few leukocyte common antigen-positive B cells compared to peripheral blood mononuclear cells. The natural killer cell activity of sinusoidal mononuclear cells was much stronger than that of peripheral blood mononuclear cells and splenic mononuclear cells. This activity was completely suppressed by the treatment with anti-asialo GM1 antibody plus complement. However, their inhibitory activity on proliferation of autologous hepatocytes was not affected by this treatment. These results suggest that the sinusoidal mononuclear cells, probably CD3+ T cells, play a role in regulation of autologous hepatocyte proliferation.
Kanai
M
M
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Inamoto
T
T
Kanaya
S
S
Kinoshita
K
K
Fujimura
N
N
Yamauchi
A
A
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D017364:Collagenases / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D005677:G(M1) Ganglioside / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D008099:Liver / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology* / Q000502:physiology; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology
D005677:G(M1) Ganglioside; D004815:Epidermal Growth Factor; C018835:asialo GM1 ganglioside; D017364:Collagenases