Thyrotropin regulation of sialic acid expression in rat thyroid cells.
The present study, utilizing the hormone-responsive rat thyroid cell line FRTL-5, presents evidence establishing the regulatory role of thyrotropin in modulating mRNA for beta-galactoside alpha 2,6-sialytransferase, the enzyme responsible for the expression of alpha 2,6-linked sialic acid. Both the cell surface membrane and the thyroglobulin secreted by cells grown in the presence of this hormone exhibit a marked decrease in the level of alpha 2,6-bound sialic acid with little or no change in the number of alpha 2,3-sialic acid residues. An additional, and unexpected, sequel is the finding of a coordinated decrease in all of the core monosaccharide constituents of the secreted thyroglobulin. Both of the above phenomenological changes appear to be at some variance with previously described systems wherein thyrotropin was deemed to increase glycosylation. It is anticipated that further resolution of this apparent difference may provide a clearer definition for the role of the carbohydrate moiety in affecting the biological function of thyroglobulin.
Grollman
E F
EF
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Saji
M
M
Shimura
Y
Y
Lau
J T
JT
Ashwell
G
G
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002241:Carbohydrates / Q000032:analysis; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012794:Sialic Acids / Q000032:analysis / Q000378:metabolism*; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013954:Thyroglobulin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D002241:Carbohydrates; D012333:RNA, Messenger; D012794:Sialic Acids; D013972:Thyrotropin; D013954:Thyroglobulin; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase