How much hydration is necessary for the stabilisation of DNA-duplex?
A combination of NOESY and ROESY experiments show that the higher stabilities (T(m)) of phenazine tethered matched (2) and G-A mismatched (4) DNA duplexes are due to the decrease of the exchange-rate (i.e. increase of the life-time) of the imino-protons and the reduced water activity in their minor grooves compared to their non-tethered counterparts (1) and (3).
Maltseva
T V
TV
Department of Bioorganic Chemistry, University of Uppsala, Sweden.
Agback
P
P
Chattopadhyaya
J
J
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010619:Phenazines; D011522:Protons; D014867:Water / Q000737:chemistry*
D010619:Phenazines; D011522:Protons; D014867:Water; D004247:DNA