Chloroquine stabilization of phospholipid membranes against diacylglycerol-induced perturbation.
The effects of 1-stearoyl,2-sn-arachidonoylglycerol (SAG) and the antimalarial drug chloroquine on lipid bilayer structure were studied by 2H-NMR spectroscopy. Model lipid systems were established with compositions similar to those of normal human erythrocytes, malaria-infected erythrocytes, or malaria parasite membranes. The 2H-NMR spectra of the membranes formed from the lipids extracted from normal human erythrocytes were similar to those obtained using the corresponding lipid mixtures. The order parameters of the model "infected" and model "parasite" membranes were reduced markedly relative to that of normal erythrocytes. Addition of SAG induced formation of non-bilayer lipid phases in all lipid systems. Only a small decrease in the order parameters of the acyl side chains of the phosphatidylserine, but not of the phosphatidylcholine component of the lipid membranes, was observed upon the addition of chloroquine. A dramatic effect was observed upon the addition of chloroquine to the SAG-containing membranes: this antimalarial almost totally abolished the formation of SAG-induced non-bilayer lipid phases. Since SAG, endogenously formed in erythrocyte membranes, is a potent activator of phospholipase A2, this membrane-stabilizing action of chloroquine may partially account for the phospholipase A2-inhibiting properties of this drug, and, consequently, for both its therapeutic and toxic modes of action.
Zidovetzki
R
R
Department of Biology, University of California, Riverside 92521.
Sherman
I W
IW
Cardenas
M
M
Borchardt
D B
DB
D002738:Chloroquine / Q000737:chemistry / Q000494:pharmacology*; D004075:Diglycerides / Q000494:pharmacology*; D004347:Drug Interactions; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000187:drug effects; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D010743:Phospholipids / Q000097:blood*
D004075:Diglycerides; D008051:Lipid Bilayers; D010743:Phospholipids; C075809:1-stearoyl-2-arachidonoylglycerol; D002738:Chloroquine; D010741:Phospholipases A; D054467:Phospholipases A2