Concentration-dependent effect of iron on gamma-linolenic acid toxicity in ZR-75-1 human breast tumor cells in culture.
Polyunsaturated fatty acids are cytotoxic to ZR-75-1 human breast tumor cells in culture. This effect may be potentiated by the simultaneous addition of iron. When cytotoxicity was measured in the presence of different concentrations of both gamma-linolenic acid and ferrous chloride there was an increase in cell death above concentrations of 9 microM and 0.05 microM, respectively. The potentiation of the effects of 18:3n-6 at low concentrations by the simultaneous addition of Fe(II) ions supports the contention that an alteration in the intracellular Fe(II)/Fe(III) ratio is necessary to promote autocatalytic lipid peroxidation.
Cantrill
R C
RC
Efamol Research Institute, Kentville, Nova Scotia, Canada.
Ells
G
G
Chisholm
K
K
Horrobin
D F
DF
D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation; D014407:Tumor Cells, Cultured; D017965:gamma-Linolenic Acid / Q000633:toxicity*
D017965:gamma-Linolenic Acid; D007501:Iron