Characterization of the 97 and 103 kDa forms of starch branching enzyme from potato tubers.
N-Terminal analysis, peptide mapping and partial peptide sequencing of the 97 and 103 kDa forms of starch branching enzyme from potato tubers showed that the two forms are highly related. A comparison with sequence data in the literature showed that these forms belong to the starch branching enzyme isoform I family. An internal cDNA fragment was obtained using PCR technology on potato tuber RNA with two oligonucleotide primers constructed from the peptide sequence data. Southern blot analysis using the PCR fragment as probe showed that there is only one gene locus encoding this isoform of the enzyme in Solanum tuberosum as well as in Solanum commersonii.
Khoshnoodi
J
J
Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences.
Ek
B
B
Rask
L
L
Larsson
H
H
D015061:1,4-alpha-Glucan Branching Enzyme / Q000737:chemistry*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary; D007527:Isoenzymes / Q000737:chemistry*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000201:enzymology*
D018076:DNA, Complementary; D007527:Isoenzymes; D015061:1,4-alpha-Glucan Branching Enzyme