Chicken IgY-Fc antibody elicited in a rabbit as first coat on frosted glass beads in immunoassays.
The observation of Esteves and Binaghi that the Fc moieties of mammalian IgG are antigenically similar but antigenically unrelated to the Fc fraction of chicken IgG (IgY) was usefully applied in enzyme linked immuno-sorbent assay (ELISA). In this communication its use in ELISA of two plant viruses viz. tobacco mosaic virus (common strain) and grape vine A virus is illustrated. Frosted glass beads were used as reagent carriers and the reactions were conducted in the wells of multi-titre plates.
Polson
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Department Gynaecology and Obstetrics, Tygerberg Hospital, Republic of South Africa.
D000818:Animals; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007141:Immunoglobulin Fc Fragments / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology*; D008863:Microspheres; D010942:Plant Viruses / Q000276:immunology; D011817:Rabbits
C016512:IgY; D007141:Immunoglobulin Fc Fragments; D007136:Immunoglobulins