title: Immunocytologic analysis of cells obtained from bronchoalveolar lavage in a model of rat lung allograft rejection.
abstract: Left lung transplantation (n = 30) between BN rat (RT1n) and LEW rat (RT1(1)) was performed to examine serial changes in the inflammatory cell profile and T-cell subsets occurring in bronchoalveolar lavage (BAL) obtained after transplantation. Transplanted animals were sacrificed on Days 1 to 7 post-transplantation. Previous studies show that lung allografts between these rat strains were strongly rejected within 7 days. The serial change in the cell profile of BAL showed a marked initial predominance of polymorphonuclear leukocytes, a decrease in macrophages, and a temporary increase in number of eosinophils on Day 2 post-transplantation. A gradual increase in lymphocytes coincident with progression of rejection was also noted. Immunocytologic studies using monoclonal antibodies specific for rat T-cell subsets and interleukin-2 receptor (IL-2R) showed significant increase in pan-T-cells on Days 3, 4, and 5 and T-suppressor/cytotoxic (CD8 positive) fraction on Days 4 and 5, whereas the T-helper (CD4 positive) fraction peaked on Day 2. The frequency of T-cells expressing IL-2R (55 kDa), indicating activated T-cells, significantly increased as early as Day 2 and maintained its high value thereafter. mRNA levels for IL-2R were detectable in the allografts on Day 2 and peaked on Day 5 post-transplantation. The value of CD4/CD8 T-cell ratios rose initially and then dropped below 1.0 on Days 4 and 5. These values differed markedly from those of syngeneic transplants (Lew-->Lew) examined on Day 5 post-transplantation. First, no significant changes in BAL cytology were seen when syngeneic transplants were compared with normal (Lew) lung.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cardiothoracic Surgery, Cedars-Sinai Medical Center, Los Angeles, California 90048.
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: G D
authors.initials: GD
authors.lastname: Wu
authors.forename: R
authors.initials: R
authors.lastname: Saito
authors.forename: A M
authors.initials: AM
authors.lastname: Marchevsky
authors.forename: J
authors.initials: J
authors.lastname: Prehn
authors.forename: J M
authors.initials: JM
authors.lastname: Matloff
authors.forename: P F
authors.initials: PF
authors.lastname: Waters
authors.forename: S C
authors.initials: SC
authors.lastname: Jordan
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D016516:CD4-CD8 Ratio; D004804:Eosinophils / Q000473:pathology; D006084:Graft Rejection / Q000473:pathology*; D007958:Leukocyte Count; D016040:Lung Transplantation; D008264:Macrophages / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D015375:Receptors, Interleukin-2 / Q000235:genetics; D013601:T-Lymphocytes / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D015375:Receptors, Interleukin-2