Renal and single-nephron function is comparable in thiobutabarbitone- and thiopentone-anaesthetised rats.
The thiobutabarbitone(TB, Inactin)-anaesthetised rat is an extremely widely used preparation for the study of renal function at the whole-organ and nephron levels. The recent withdrawal of TB from the market has made it essential to find an anaesthetic producing experimental conditions as similar as possible to TB to allow comparison of past and future data. Blood gas analysis, clearance and micropuncture studies were therefore performed in rats anaesthetised with TB or the related thiobarbiturate thiopentone (TP) (both 100 mg/kg body weight) to establish whether the latter meets this requirement. Both barbiturates caused similar transient respiratory depression and acidosis. Mean values (TP versus TB) over the total 8-h observation period for glomerular filtration rate (0.94 versus 1.05 ml/min), urine flow (3.8 versus 4.4 microliters/min) and K+ excretion (0.98 versus 1.18 mumol/min) were slightly lower (P < 0.05) in TP rats, whereas renal blood flow (6.26 versus 6.24 ml/min), filtration fraction (0.31 versus 0.34) and Na+ excretion (0.11 versus 0.098 mumol/min) did not differ. The single-nephron filtration rate (SNGFR) (42.1 versus 41.1 nl/min) and fractional reabsorption (42% versus 47%), both measured in the proximal tubule, did not differ, although in the TP group SNGFR rose with time (4.4%/h) whereas the fractional reabsorption did not change significantly; in the TB group SNGFR was constant but fractional reabsorption declined with time (1.5%/h). Fractional reabsorption up to the distal convoluted tubule declined with time, this was more pronounced in the TP group. SNGFR measured at this site did not differ between TP and TB (30.3 versus 30.1 nl/min) but increased with time with TP (2.7%/h).(ABSTRACT TRUNCATED AT 250 WORDS)
Häberle
D A
DA
Department of Physiology, University of Munich, Germany.
Davis
J M
JM
Kawabata
M
M
Metz
C
C
Wapler
P
P
Stachl
M
M
D000136:Acid-Base Equilibrium / Q000187:drug effects; D000758:Anesthesia; D000818:Animals; D001784:Blood Gas Analysis; D007668:Kidney / Q000187:drug effects*; D007678:Kidney Glomerulus; D008297:Male; D009399:Nephrons / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects; D013874:Thiopental / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D014563:Urodynamics / Q000187:drug effects
C004316:thiobutabarbital; D013874:Thiopental