Nucleotide and deduced amino acid sequence of porcine interleukin 4 cDNA derived from lamina propria lymphocytes.
Total RNA was isolated from in vitro activated lamina propria lymphocytes and used to direct the synthesis of cDNA. Interleukin 4 transcripts were then specifically amplified by PCR. Comparison of the nucleotide sequence with its human homologue demonstrates deletion within the coding region of pig interleukin 4 centred around amino acid residue 70 in the mature human protein.
Bailey
M
M
Department of Veterinary Medicine, University of Bristol, UK.
Perry
A C
AC
Bland
P W
PW
Stokes
C R
CR
Hall
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D016415:Sequence Alignment; D013045:Species Specificity; D013552:Swine
D012333:RNA, Messenger; D015847:Interleukin-4; D004247:DNA