title: Crystallization of pancreatic procolipase and of its complex with pancreatic lipase.
abstract: The human pancreatic lipase-porcine procolipase complex has been crystallized in space group P3(2)21 (a = b = 80.3 A and c = 251 A) from a solution containing polyethylene glycol, NaCl and beta-octyl glucoside. The crystals diffract to 2.6 A on a synchrotron beam. The complex in the presence of bile salts and phospholipids crystallizes in a tetragonal space group P4(2)2(1)2 (a = b = 133.4 A, c = 92.6 A). Crystals of procolipase alone were obtained under slightly different experimental conditions (space group I432, a = b = c = 164.3 A).
authors.affiliation: Laboratoire de Cristallographie et de Cristallisation des Macromolécules Biologiques, URA1296-CNRS, Faculté de Médecine Secteur-Nord, Marseille, France.
authors.forename: H
authors.initials: H
authors.lastname: van Tilbeurgh
authors.forename: Y
authors.initials: Y
authors.lastname: Gargouri
authors.forename: C
authors.initials: C
authors.lastname: Dezan
authors.forename: M P
authors.initials: MP
authors.lastname: Egloff
authors.forename: M P
authors.initials: MP
authors.lastname: Nésa
authors.forename: N
authors.initials: N
authors.lastname: Ruganie
authors.forename: L
authors.initials: L
authors.lastname: Sarda
authors.forename: R
authors.initials: R
authors.lastname: Verger
authors.forename: C
authors.initials: C
authors.lastname: Cambillau
mesh_terms: D000818:Animals; D003089:Colipases / Q000737:chemistry*; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors; D006801:Humans; D008049:Lipase / Q000737:chemistry*; D010179:Pancreas / Q000201:enzymology*; D011498:Protein Precursors / Q000737:chemistry*; D013552:Swine; D014961:X-Ray Diffraction
chemical_list: D003089:Colipases; D004792:Enzyme Precursors; D011498:Protein Precursors; C037107:procolipase; D008049:Lipase