Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis.
Sequence analysis of cloned rescued DNA fragments from a Bacillus subtilis strain with an inserted recombinant plasmid in ribosomal operon rrnE revealed the presence of two tRNA genes for Met and Asp at the 3' end of the operon. Probing chromosomal DNA from a strain carrying a plasmid inserted in rrnD with a fragment containing the genetically unassigned cluster of 16 tRNA genes revealed that the cluster is located immediately following the rrnD operon. Our findings show that all 10 rrn operons in B. subtilis are associated with tRNA gene clusters.
Rudner
R
R
Department of Biological Sciences, Hunter College, City University of New York, New York 10021.
Chevrestt
A
A
Buchholz
S R
SR
Studamire
B
B
White
A M
AM
Jarvis
E D
ED
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides; D009876:Operon; D016133:Polymerase Chain Reaction / Q000379:methods; D012335:RNA, Ribosomal / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009838:Oligodeoxyribonucleotides; D012335:RNA, Ribosomal; D012343:RNA, Transfer