Iron responsive element-binding protein (IRE-BP) in leukemic cells: analysis using enzyme-linked immunosorbent assay and semiquantitative polymerase chain reaction.
The level of iron responsive element-binding protein (IRE-BP) in leukemic cells, which is essential for iron homeostasis and plays an important role in cell metabolism and cell growth, was measured using an enzyme-linked immunosorbent assay (ELISA). Comparing the levels in different clinical stages, the levels in CML cells in the chronic phase (mean +/- S.E., 0.270 +/- 0.110 U/mg protein, n = 9) and those in AML cells (0.150 +/- 0.104 U/mg) protein, n = 21) were significantly lower than that in normal granulocytes (0.628 +/- 0.216 U/mg protein, n = 9, p < 0.001 vs CML and AML). Analysis of IRE-BP mRNA expression in leukemic cells using semiquantitative polymerase chain reaction showed suppressed expression of mRNA as compared to normal bone marrow cells. These observations suggest that there may be dysregulation of IRE-BP expression and production in leukemic cells.
Tanaka
J
J
Third Department of Internal Medicine, Hokkaido University School of Medicine, Japan.
Ogawa
T
T
Kamiya
K
K
Kobayashi
T
T
Uehara
Y
Y
Honke
K
K
Gasa
S
S
Kasai
M
M
Sakurada
K
K
Miyazaki
T
T
D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006098:Granulocytes / Q000737:chemistry; D006801:Humans; D035925:Iron-Regulatory Proteins; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood; D007951:Leukemia, Myeloid / Q000097:blood*; D015470:Leukemia, Myeloid, Acute / Q000097:blood; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000097:blood; D016601:RNA-Binding Proteins / Q000097:blood* / Q000235:genetics
D035925:Iron-Regulatory Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins