title: Biochemical analysis of a mutant Tetrahymena lacking outer dynein arms.
abstract: Tetrahymena thermophila mutants homozygous for the oad mutation become nonmotile when grown at the restrictive temperature, and axonemes isolated from nonmotile mutants lack approximately 90% of their outer dynein arms. Electrophoretic analyses of axonemes isolated from nonmotile mutants (oad axonemes) indicate they contain significantly fewer of the 22 S dynein heavy chains that axonemes isolated from wild-type cells (wild-type axonemes) contain. The 22 S dynein heavy chains that remain in axonemes isolated from nonmotile, oad mutants are assembled into 22 S dynein particles that exhibit wild-type levels of ATPase activity. Two-dimensional gel electrophoresis of oad axonemes show that they are deficient in no proteins other than those proteins thought to be components of 22 S dynein. This report is the first formal proof that outer dynein arms in Tetrahymena cilia are composed of 22 S dynein.
authors.affiliation: Department of Zoology, Miami University, Oxford, Ohio 45056.
authors.forename: S A
authors.initials: SA
authors.lastname: Ludmann
authors.forename: A
authors.initials: A
authors.lastname: Schwandt
authors.forename: X
authors.initials: X
authors.lastname: Kong
authors.forename: C S
authors.initials: CS
authors.lastname: Bricker
authors.forename: D G
authors.initials: DG
authors.lastname: Pennock
mesh_terms: D000818:Animals; D002465:Cell Movement; D004398:Dyneins / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics; D016808:Tetrahymena thermophila / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D015800:Protozoan Proteins; D004398:Dyneins