[Comparative studies of acid-base and gas metabolism in the whole blood and erythrocytes in endotoxic shock in rabbits].
Parameters of acid-base and gas metabolism in whole blood and in red cell hemolysate were determined 30, 60 and 120 minutes after inducing endotoxin shock in rabbits of Belgian giant breed. Astrup's micromethod on apparatus of the firm "Radiometer" was used. Endotoxin shock was induced by intravenous injection of 2 mg/kg endotoxin from E. coli 0111:B4 strain. Equations were used for expressing the relation between pH of erythrocytes and pH of whole blood and analysis made of the correlation between the changes in the individual components of erythrocyte and of whole blood acid-base metabolism. Severe metabolic acidosis developed both in whole arterial blood, in mixed venous blood and in red cell hemolysate. Close correlation was recorded between the changes in the parameters of acid-base metabolism in whole blood and in red cell hemolysate on the 60. and especially on the 120. minute after endotoxin administration. The blood gas changes manifested by slight decrease of PCO2 and PO2 both in arterial and in mixed venous whole blood and red cell hemolysate were not statistically significant. The severe tissue hypoxia during the early phases of endotoxin shock was thought to be the result of severe hemodynamic changes.
Ianev
E
E
Tsareva
M
M
Delibasheva
A
A
Kenarov
P
P
D000136:Acid-Base Equilibrium; D000138:Acidosis / Q000097:blood; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000097:blood*; D010100:Oxygen / Q000097:blood*; D011817:Rabbits; D012772:Shock, Septic / Q000097:blood*; D013997:Time Factors
D002245:Carbon Dioxide; D010100:Oxygen