Combined immunocytochemistry and enzyme cytochemistry on ultra-thin cryosections: a new method.
We combined immunocytochemistry and enzyme cytochemistry to localize two different proteins on the same ultrathin cryosection. In this method the immunocytochemical localization is visualized with colloidal gold probes and the enzyme cytochemical detection is achieved with cerium as the capture agent. The immunocytochemistry is conducted first so that any potential adverse effects of the enzyme cytochemical procedure will not alter the antibody binding properties of the cryosections.
Takizawa
T
T
Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus.
Robinson
J M
JM
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000648:ultrastructure; D002563:Cerium; D016616:Cryoultramicrotomy; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007781:Lactoferrin / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D009504:Neutrophils / Q000378:metabolism* / Q000648:ultrastructure
D002563:Cerium; D000469:Alkaline Phosphatase; D007781:Lactoferrin