Analysis of 70 KD heat shock protein (HSP70) expression in the lesional skin of lupus erythematosus (LE) and LE related diseases.
Biopsied specimens from skin lesions of SLE were studied for expression of 70 KD heat shock protein (HSP70). The pattern of HSP70 expression in SLE was diffuse in whole epidermis, hair follicles, and sweat gland cells and rather more intense than that in other control groups or normal skin. No significant differences in HSP70 expression were observed between sun-exposed and protected areas of SLE skin lesions. Unlike SLE, reduced or no expression of HSP70 was observed in skin lesions of DLE. In tissue culture, UVB radiation in vitro induced relatively intense expression of HSP70 in the nuclear area of keratinocytes. A few gamma delta T cell receptor positive cells which might respond to HSP70 expressing cells were detected in the basal layer of skin lesions of diseases. These studies suggest that aberrant expression of HSP70 in skin lesions of SLE might contribute to both skin lesions and antibody formation in SLE.
Ghoreishi
M
M
Department of Dermatology, School of Medicine, Tokyo Medical and Dental University, Japan.
Katayama
I
I
Yokozeki
H
H
Nishioka
K
K
D000293:Adolescent; D000328:Adult; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004817:Epidermis / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects; D005260:Female; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000235:genetics / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000473:pathology / Q000502:physiology / Q000528:radiation effects; D008179:Lupus Erythematosus, Discoid / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D012867:Skin / Q000473:pathology* / Q000503:physiopathology / Q000528:radiation effects; D012871:Skin Diseases / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D014466:Ultraviolet Rays
D006360:Heat-Shock Proteins