Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.
Monoclonal antibodies raised to a number of microfilament-associated proteins were shown to recognize the appropriate proteins in extracts from human colon tissue. They were then used in an immunohistochemical study of normal colonic mucosa, adenomas, and adenocarcinomas. A strong reaction was seen in stromal cells within the tumours (both adenomas and adenocarcinomas) when frozen sections were stained with antibodies to filamin and caldesmon. In addition, a similar reaction was seen in the adenocarcinomas when stained with antibodies to talin and gelsolin. We believe that immunohistochemical staining with these antibodies reveals a tumour-induced process in the surrounding cells, possibly related to a host response to tumours.
Porter
R M
RM
Department of Surgery, University of Dundee, Ninewells Hospital and Medical School, U.K.
Holme
T C
TC
Newman
E L
EL
Hopwood
D
D
Wilkinson
J M
JM
Cuschieri
A
A
D000230:Adenocarcinoma / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002148:Calmodulin-Binding Proteins / Q000276:immunology; D003106:Colon / Q000737:chemistry; D003110:Colonic Neoplasms / Q000378:metabolism*; D003285:Contractile Proteins / Q000276:immunology; D064448:Filamins; D018260:Gelsolin / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000737:chemistry; D008840:Microfilament Proteins / Q000276:immunology*; D016608:Talin / Q000276:immunology
D000911:Antibodies, Monoclonal; D002148:Calmodulin-Binding Proteins; D003285:Contractile Proteins; D064448:Filamins; D018260:Gelsolin; D008840:Microfilament Proteins; D016608:Talin