title: Immunoglobulin G subclass responses to mycobacterial lipoarabinomannan in HIV-infected and non-infected patients with tuberculosis.
abstract: Immunoglobulin G subclass responses to lipoarabinomannan (LAM) of Mycobacterium tuberculosis were determined by ELISA in both HIV-1 antibody positive (n = 31) and negative (n = 43) patients with tuberculosis (TB). Responses were also studied in a group of healthy controls (n = 16) and HIV-1 antibody positive (n = 60) individuals without TB. IgG2 antibodies were the predominant subclass, being present in 25 of 43 non-HIV-infected TB patients (58%) and in 11 of 31 HIV-infected TB patients (35%). However, HIV+ TB patients also showed IgG4 (n = 16; 52%), and IgG1 (n = 4, 13%) responses to LAM, whereas these subclasses were absent in sera from HIV-TB patients. Individuals in both non-tuberculous control groups showed no antibody responses to LAM. The influence of HIV infection on B cell responses to LAM, and possible mechanisms for antibody-mediated regulation of immunity to TB, are explored.
authors.affiliation: Department of Clinical Sciences, London School of Hygiene & Tropical Medicine, UK.
authors.forename: C T
authors.initials: CT
authors.lastname: Da Costa
authors.forename: S
authors.initials: S
authors.lastname: Khanolkar-Young
authors.forename: A M
authors.initials: AM
authors.lastname: Elliott
authors.forename: K M
authors.initials: KM
authors.lastname: Wasunna
authors.forename: K P
authors.initials: KP
authors.lastname: McAdam
mesh_terms: D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D009161:Mycobacterium / Q000276:immunology*; D012737:Sex Factors; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; C050016:lipoarabinomannan