title: Sequence-specific binding of [N-MeCys3,N-MeCys7]TANDEM to TpA.
abstract: The sequence selective binding of [N-MeCys3,N-MeCys7]TANDEM to DNA has been studied by footprinting experiments on DNA fragments containing the self-complementary sequences CGCGATATCGCG, CGCGTATACGCG, CGCGTTAACGCG and CGCGAATTCGCG. DNAase I and micrococcal nuclease reveal drug-induced footprints with the central sequences ATAT, TATA and TTAA, but not AATT, suggesting that the ligand binds to the dinucleotide TpA. The ligand renders certain adenines hyper-reactive to diethyl pyrocarbonate. These are observed with ATAT, TATA and TTAA, but not AATT, and are located both within, and distal to, the TpA-binding sites.
authors.affiliation: Department of Physiology and Pharmacology, University of Southampton, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Lavesa
authors.forename: R K
authors.initials: RK
authors.lastname: Olsen
authors.forename: K R
authors.initials: KR
authors.lastname: Fox
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D015247:Deoxyribonuclease HindIII; D003850:Deoxyribonuclease I; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D007202:Indicators and Reagents; D007364:Intercalating Agents; D008836:Micrococcal Nuclease; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011810:Quinoxalines; D013329:Structure-Activity Relationship
chemical_list: D015226:Dinucleoside Phosphates; D007202:Indicators and Reagents; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D011810:Quinoxalines; C075619:(N-MeCys(3)-N-MeCys(7))TANDEM; C028145:2'-deoxythymidylyl-(3'-5')-2'-deoxyadenosine; D004247:DNA; D015247:Deoxyribonuclease HindIII; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease