A specific and rapid receptor assay for squamous-cell carcinoma of human esophagus.
A specific radioreceptor assay, for squamous-cell carcinoma of the human esophagus, using 2G3 monoclonal antibody, has been developed for the first time. In this assay a new mathematical parameter of the Scatchard plot has been introduced for the correct measure of binding capacity (BCcm = x cos alpha). This is the perpendicular distance from the origin to the Scatchard plot line. It is always a positive quantity and is directly proportional to both the x and y intercepts for expressing the binding capacity. The assay is highly sensitive and can be used to differentiate various types of esophageal tumors such as squamous-cell carcinoma, adenocarcinoma, ulcerative growth and also a benign growth. BCcm significantly varies in the case of squamous-cell carcinoma tumors compared to adenocarcinoma and other types of tumor of the human esophagus. The assay can be completed in about 4 h and it may be used as a clinical diagnostic test.
Panse
G T
GT
Immuno-Biochem Laboratory, Tata Memorial Hospital, Bombay, India.
Nadkarni
J S
JS
Nadkarni
J J
JJ
D000230:Adenocarcinoma / Q000737:chemistry; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D004938:Esophageal Neoplasms / Q000737:chemistry*; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000032:analysis*; D012680:Sensitivity and Specificity
D011956:Receptors, Cell Surface