A study on retinal photoisomerization catalyzed by a protein from the honeybee visual system.
The photoisomerization of all-trans retinal catalyzed by an isomerase purified from the honeybee retina is stereospecifically directed toward the formation of 11-cis retinal, the isomer required for rhodopsin regeneration. A model of the enzymatic reactions was developed, which fits the experimental data. In a particular experiment where irradiation was performed with a LASER light, the rate constants of the photoisomerization of all-trans retinal were dependent on the light intensity and were therefore greatly enhanced.
Cugnoli
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Istituto di Cibernetica e Biofisica del CNR.
Fioravanti
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Pepe
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D000818:Animals; D001516:Bees; D002384:Catalysis; D007535:Isomerases / Q000378:metabolism*; D007536:Isomerism; D007700:Kinetics; D007834:Lasers; D008027:Light; D010777:Photochemistry; D012160:Retina / Q000201:enzymology / Q000378:metabolism*; D012172:Retinaldehyde / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism*; D019745:cis-trans-Isomerases
C077124:retinal isomerase; D014212:Tretinoin; D007535:Isomerases; D019745:cis-trans-Isomerases; D012172:Retinaldehyde