The random amplification of polymorphic DNA allows the identification of strains and species of schistosome.
The use of arbitrarily selected primers (10-24 nucleotides) and very low stringency annealing conditions (30 degrees C followed by 40 degrees C) for the polymerase chain reaction amplification of 1.0 ng of schistosome DNA resulted in relatively complex patterns of products. Amongst the primers tested some, for example 5'-TCGTAGCCAA, produced patterns that included bands that were polymorphic between strains of Schistosoma mansoni. Other primers, for example 5'-TCACGATGCA, produced apparently identical products using DNA from 5 S. mansoni strains but highly variable patterns when DNA from different schistosome species was used. The results indicate that the random amplification of polymorphic DNA (RAPD) may be an extremely useful approach to the identification of schistosome strains and species.
Dias Neto
E
E
Centro de Pesquisas René Rachou, Belo Horizonte, Minas Gerais, Brazil.
de Souza
C P
CP
Rollinson
D
D
Katz
N
N
Pena
S D
SD
Simpson
A J
AJ
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000235:genetics; D005784:Gene Amplification; D017238:Genes, Helminth; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012547:Schistosoma / Q000145:classification / Q000235:genetics*; D012550:Schistosoma mansoni / Q000145:classification / Q000235:genetics; D013045:Species Specificity
D015342:DNA Probes; D004275:DNA, Ribosomal; D004247:DNA