Direct sequencing of consensus primer generated PCR fragments of human papillomaviruses.
Consensus primers specific for the L1 and E1 regions were used to amplify HPV DNA fragments. These fragments were then directly sequenced using the consensus primers as the sequencing primers. The linking of PCR amplification, direct sequencing, and computer data bank analysis provides a new approach in the detection of different HPVs and has several advantages over existing methodology. These advantages include increased precision in the rapid characterization of known HPVs, detection of mutations, and identification of new HPV types.
Rady
P L
PL
Department of Microbiology, University of Texas, Medical Branch, Galveston 77550.
Chin
R
R
Arany
I
I
Hughes
T K
TK
Tyring
S K
SK
D001483:Base Sequence; D016384:Consensus Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D016208:Databases, Factual; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D014412:Tumor Virus Infections / Q000382:microbiology
D017931:DNA Primers; D004279:DNA, Viral