title: Evidence that the Rad1 and Rad10 proteins of Saccharomyces cerevisiae participate as a complex in nucleotide excision repair of UV radiation damage.
abstract: A newly characterized rad1 missense mutation (rad1-20) in the yeast Saccharomyces cerevisiae maps to a region of the Rad1 polypeptide known to be required for Rad1-Rad10 complex formation. The UV sensitivity of the rad1-20 mutant can be partially and specifically corrected by overexpression of wild-type Rad10 protein. These results suggest that complex formation between the Rad1 and Rad10 proteins is required for nucleotide excision repair.
authors.affiliation: Department of Pathology, University of Texas, Southwestern Medical Center at Dallas 75235.
authors.forename: W
authors.initials: W
authors.lastname: Siede
authors.forename: A S
authors.initials: AS
authors.lastname: Friedberg
authors.forename: E C
authors.initials: EC
authors.lastname: Friedberg
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004249:DNA Damage; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins; D004307:Dose-Response Relationship, Radiation; D004720:Endonucleases; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D016366:Open Reading Frames; D017354:Point Mutation; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D029701:Saccharomyces cerevisiae Proteins; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014466:Ultraviolet Rays
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D004720:Endonucleases; C069266:RAD1 protein, S cerevisiae; C064162:RAD10 protein, S cerevisiae; D015719:Single-Strand Specific DNA and RNA Endonucleases; D045643:DNA Repair Enzymes