Specific T-cell recognition of the merozoite proteins rhoptry-associated protein 1 and erythrocyte-binding antigen 1 of Plasmodium falciparum.
The merozoite proteins merozoite surface protein 1 (MSP-1) and rhoptry-associated protein 1 (RAP-1) and synthetic peptides containing sequences of MSP-1, RAP-1, and erythrocyte-binding antigen 1, induced in vitro proliferative responses of lymphocytes collected from Ghanaian blood donors living in an area with a high rate of transmission of malaria. Lymphocytes from a large proportion of the Ghanaian blood donors proliferated in response to the RAP-1 peptide, unlike those of Danish control blood donors, indicating that this sequence contains a malaria-specific T-cell epitope broadly recognized by individuals living in an area with a high transmission rate of malaria. Most of the donor plasma samples tested contained immunoglobulin G (IgG) and IgM antibodies recognizing the merozoite proteins, while only a minority showed high IgG reactivity to the synthetic peptides.
Jakobsen
P H
PH
Department of Infectious Diseases, University Hospital, Copenhagen, Denmark.
Hviid
L
L
Theander
T G
TG
Afare
E A
EA
Ridley
R G
RG
Heegaard
P M
PM
Stuber
D
D
Dalsgaard
K
K
Nkrumah
F K
FK
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D000953:Antigens, Protozoan; D000954:Antigens, Surface / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D016778:Malaria, Falciparum / Q000276:immunology*; D020066:Merozoite Surface Protein 1; D008875:Middle Aged; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology*; D011956:Receptors, Cell Surface; D013601:T-Lymphocytes / Q000276:immunology*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D002352:Carrier Proteins; C066001:Duffy antigen binding protein, Plasmodium; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; C065352:rhoptry associated protein, Plasmodium