Crystallographic characterization of a PHO4-DNA complex.
Crystals have been obtained of the DNA-binding domain of the yeast transcription factor PHO4 in complexes with several synthetic fragments of DNA with appropriate cognate sequences. Crystals suitable for X-ray diffraction studies were produced in the case of a complex of the protein with a 17 base-pair fragment of DNA from a solution in polyethylene glycol and calcium chloride. The crystals have the space group of P4(1)2(1)2 or P4(3)2(1)2 with unit cell dimensions a = b = 56.7 A, c = 447.8 A. The diffraction data at 3 A resolution were collected using synchrotron radiation with a Weissenberg camera for macromolecular crystallography.
Hakoshima
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Teranishi
Y
Y
Ohira
T
T
Suzuki
K
K
Shimizu
M
M
Shirakawa
M
M
Kyogoku
Y
Y
Ogawa
N
N
Oshima
Y
Y
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry*; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014961:X-Ray Diffraction
D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C060292:PHO4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors