The effect of epinephrine on the intracellular free calcium of parent and Ki-ras-transfected NIH3T3 cells.
Here we describe differences in the formation of the epinephrine-induced Ca2+ transients between parent and Ki-ras-transformed NIH3T3 fibroblasts. These transients proved to be the results of an efflux from both the thapsigargin-sensitive and -insensitive intracellular pools. While the epinephrine-induced detachment of the ras-transformed cells might be due to cytoskeletal and/or cell-matrix alterations.
Tóth
S
S
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Fu
T
T
Nozawa
Y
Y
Csaba
G
G
D016475:3T3 Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D003599:Cytoskeleton / Q000502:physiology; D004837:Epinephrine / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000502:physiology; D011905:Genes, ras / Q000235:genetics / Q000502:physiology*; D051379:Mice; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin; D014162:Transfection
D013729:Terpenes; D019284:Thapsigargin; D002118:Calcium; D004837:Epinephrine