title: The matrix region is responsible for the differential ability of two retroviruses to function as helpers for vector propagation.
abstract: We have investigated the ability of two related reticuloendotheliosis viruses to propagate a spleen necrosis virus (SNV) based retroviral vector in canine osticosarcoma (D17) cells. Reticuloendotheliosis virus strain A (REV-A) consistently propagated the vector more efficiently than SNV in cell culture. To identify the area of the viral genome responsible for the superior helper function of REV-A, we constructed chimeric viruses between SNV and REV-A. Analysis of helper function indicated that a virus comprised of the SNV genome, but containing the matrix region of REV-A, could propagate the vector as well as REV-A. Although REV-A is also a superior virus for vector propagation in chicken embryo fibroblast cells, the region of the viral genome that confers superior helper function does not map to the gag region of REV-A in this cell type.
authors.affiliation: McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
authors.forename: C R
authors.initials: CR
authors.lastname: Casella
authors.forename: A T
authors.initials: AT
authors.lastname: Panganiban
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002678:Chimera; D004285:Dogs; D005822:Genetic Vectors; D016679:Genome, Viral; D006378:Helper Viruses / Q000235:genetics* / Q000502:physiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D014763:Viral Matrix Proteins