Production of monoclonal antibodies that inhibit ADP-ribosylation of small GTP-binding proteins catalyzed by Clostridium botulinum ADP-ribosyltransferase C3.
Four monoclonal antibodies that inhibited ADP-ribosylation of 23 kDa protein(s) of ascidian eggs catalyzed by Clostridium botulinum ADP-ribosyltransferase C3 were produced. They also inhibited C3-catalyzed ADP-ribosylation of the 24 kDa protein of rat liver cytosol. By the immunoprecipitation technique, it was found that they recognized small GTP-binding proteins of ascidian eggs and mammalian brains, but did not interact with the rat brain activator of the ADP-ribosyltransferase reaction. The antibody can also immunoprecipitate recombinant Rho A irrespective as to whether the Rho A is the GDP-bound form or the GTPrS-bound form. Thus the antibodies are novel and useful tools in analyzing the physiological roles of the Rho family of GTP-binding proteins.
Toratani
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo.
Sekine
N
N
Katada
T
T
Yokosawa
H
H
D036002:ADP Ribose Transferases / Q000037:antagonists & inhibitors / Q000276:immunology*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000276:immunology; D001905:Botulinum Toxins; D003014:Clostridium botulinum / Q000201:enzymology; D004527:Egg Proteins / Q000378:metabolism; D005306:Fertilization; D019204:GTP-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D066298:In Vitro Techniques; D051381:Rats
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D004527:Egg Proteins; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins