Globin chain synthesis in hemolytic anemia reticulocytes. A case of hemoglobin Burke.
Peripheral red blood cells from an anemic patient were incubated with [14C]Leu and the labeled globin chains were analyzed by CM-cellulose column chromatography in 8M urea. The radioactivity almost equal to that of beta chain emerged between beta and alpha chains. The newly appeared materials were found to be derived from an abnormal hemoglobin (Hb), Hb Burke [beta 107(G9)Gly to Arg], by the sequence analyses of abnormal globin chain and beta globin mRNA from the patient.
Suzuki
H
H
Department of Biophysical Chemistry, Kitasato University School of Medicine, Kanagawa, Japan.
Wada
C
C
Kamata
K
K
Takahashi
E
E
Sato
N
N
Kunitomo
T
T
D000368:Aged; D000595:Amino Acid Sequence; D000743:Anemia, Hemolytic / Q000097:blood* / Q000209:etiology; D001483:Base Sequence; D005914:Globins / Q000096:biosynthesis*; D006453:Hemoglobinopathies / Q000097:blood* / Q000150:complications / Q000235:genetics; D006455:Hemoglobins, Abnormal / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007674:Kidney Diseases / Q000097:blood / Q000150:complications; D008297:Male; D008969:Molecular Sequence Data; D012156:Reticulocytes / Q000378:metabolism*
D006455:Hemoglobins, Abnormal; C052165:hemoglobin Burke; D005914:Globins