Direct observation of kinesin stepping by optical trapping interferometry.
Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.
Svoboda
K
K
Rowland Institute for Science, Cambridge, Massachusetts 02142.
Schmidt
C F
CF
Schnapp
B J
BJ
Block
S M
SM
D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D002417:Cattle; D049832:Decapodiformes; D007368:Interferometry; D016547:Kinesins / Q000502:physiology*; D007834:Lasers; D008027:Light; D008863:Microspheres; D008870:Microtubules / Q000502:physiology; D009068:Movement / Q000502:physiology; D012822:Silicon Dioxide
D012822:Silicon Dioxide; D000255:Adenosine Triphosphate; D016547:Kinesins