Mutation of amino acids within the gibbon ape leukemia virus (GALV) receptor differentially affects feline leukemia virus subgroup B, simian sarcoma-associated virus, and GALV infections.
The three type C retroviruses, gibbon ape leukemia virus (GALV), simian sarcoma-associated virus (SSAV), and feline leukemia virus subgroup B (FeLV-B), infect human cells by interacting with the same cell surface receptor, GLVR1. Using LacZ retroviral pseudotypes and murine cells transfected with mutant GLVR1 expression vectors, we show that the same 9-amino-acid region of human GLVR1 is critical for infection by the three viruses. Rat cells were not susceptible to infection by LacZ (FeLV-B) pseudotypes because of a block at the receptor level. We found multiple amino acid differences from human GLVR1 in the 9-amino-acid critical region of rat GLVR1. Expression of a human-rat chimeric GLVR1 in murine cells demonstrated that rat GLVR1 could function as a receptor for GALV and SSAV but not for FeLV-B. Substitution of human GLVR1 amino acids in the critical region of rat GLVR1 identified three amino acids as responsible for resistance to FeLV-B infection; two of these affect SSAV infection, but none affects GALV infection.
Tailor
C S
CS
Chester Beatty Laboratories, Institute of Cancer Research, London, England.
Takeuchi
Y
Y
O'Hara
B
B
Johann
S V
SV
Weiss
R A
RA
Collins
M K
MK
D000595:Amino Acid Sequence; D000818:Animals; D004252:DNA Mutational Analysis; D006801:Humans; D005256:Leukemia Virus, Feline / Q000254:growth & development*; D017989:Leukemia Virus, Gibbon Ape / Q000254:growth & development*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D051381:Rats; D011991:Receptors, Virus / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012842:Sarcoma Virus, Woolly Monkey / Q000254:growth & development*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; C067981:leukemia virus receptor, gibbon ape