title: Collapsin: a protein in brain that induces the collapse and paralysis of neuronal growth cones.
abstract: Repulsive guidance cues can steer neuronal growth cones during development and prevent mature axons from regenerating. We have identified a 100 kd glycoprotein in the chick brain that is a good candidate for a repulsive cue. Since it induces the collapse and paralysis of neuronal growth cones in vitro, we have named it collapsin. It is effective at concentrations of approximately 10 pM. The C-terminal half of collapsin contains a single immunoglobulin-like domain and an additional highly basic region. The N-terminal half of collapsin shares significant homology with fasciclin IV, a growth cone guidance protein in grasshopper. Recombinant collapsin causes sensory ganglion growth cones to collapse but not retinal ganglion cell growth cones. We propose that collapsin could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism.
authors.affiliation: Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors.forename: Y
authors.initials: Y
authors.lastname: Luo
authors.forename: D
authors.initials: D
authors.lastname: Raible
authors.forename: J A
authors.initials: JA
authors.lastname: Raper
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002478:Cells, Cultured; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005724:Ganglia / Q000254:growth & development; D017950:Ganglia, Sensory / Q000254:growth & development; D006023:Glycoproteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology*; D009924:Organ Culture Techniques; D009928:Organ Specificity; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D012160:Retina / Q000254:growth & development; D040121:Semaphorin-3A; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; D011994:Recombinant Proteins; D040121:Semaphorin-3A