Two components in the adrenal nicotinic secretory response revealed by cobalt ramps.
Prolonged stimulation with nicotine (50 microM) enhanced the secretion of catecholamines from perfused cat adrenal glands. The profile of secretion consisted of a quick activation phase to a peak of 7.68 micrograms/min followed by a second inactivation phase which exhibited a t1/2 of 3.75 min. Sustained stimulation with a solution enriched in K+ (59 mM) also evoked a transient secretory response, with a peak release of 8.62 micrograms/2 min and a t1/2 for inactivation of 4.8 min. Co2+ (10 mM) blocked the nicotinic response by 58% and the K(+)-evoked secretory response by over 96%. In the presence of Co2+ (5 mM), continuous perfusion with nicotine produced a transient but large initial secretory response; the gradual decrease of the extracellular Co2+ concentration, [Co2+]0, as a continuous ramp allowed the development of a second component of secretion which inactivated later on. When the glands were continuously stimulated with 59 mM K+ in the presence of Co2+, the first component of secretion was missing; the second component appeared as [Co2+]0 decreases as a ramp. In similar experiments performed in low-Na+ solution (10 mM Na+), only the first secretion component evoked by nicotine was observed. This finding suggests that the second component of secretion depends on Na+ entry through the nicotinic receptor, on the ensuing cell depolarization and on Ca2+ entry through voltage-dependent Ca2+ channels.(ABSTRACT TRUNCATED AT 250 WORDS)
Montiel
C
C
Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Artalejo
A R
AR
Sánchez-García
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P
García
A G
AG
D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002415:Cats; D003035:Cobalt / Q000378:metabolism; D005260:Female; D006207:Half-Life; D066298:In Vitro Techniques; D008297:Male; D009538:Nicotine / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000502:physiology
D002395:Catecholamines; D003035:Cobalt; D009538:Nicotine; D012964:Sodium; D011188:Potassium