Sequencing of leucocidin R from Staphylococcus aureus P83 suggests that staphylococcal leucocidins and gamma-hemolysin are members of a single, two-component family of toxins.
A 2,813-bp HincII-ClaI DNA fragment encodes the two S and F components (LukS-R and LukF-R) of leucocidin R (Luk-R) which are secreted by Staphylococcus aureus P83. The two genes (lukS-R and lukF-R) belong to a single operon. Two peptidic sequences were deduced: LukS-R is a 35,721-Da polypeptide of 315 amino acids, including a signal sequence of 29 residues, and LukF-R is a 36,838-Da polypeptide of 325 amino acids, including a signal sequence of 25 residues. LukS-R and LukF-R were expressed in Escherichia coli and purified from the periplasmic space. Luk-R exerts biological activities on polymorphonuclear cells and on erythrocytes from various animals. Comparison of the amino acid sequence of LukF-R with that of the B component of gamma-hemolysin (HlgB), those of the F and S components of another recently sequenced staphylococcal leucocidin, and those of a few peptides of the F component from Panton-Valentine leucocidin suggests that all four toxins belong to a single, two-component family of toxins.
Supersac
G
G
Institut de Bactériologie de la Faculté de Médecine, Strasbourg, France.
Prevost
G
G
Piemont
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003062:Codon; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity; D006801:Humans; D007956:Leukocidins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000737:chemistry; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000737:chemistry*; D014158:Transcription, Genetic
D001427:Bacterial Toxins; D003062:Codon; D006460:Hemolysin Proteins; D007956:Leukocidins; D011994:Recombinant Proteins