Measurement of binding kinetics using the gel electrophoresis mobility shift assay.
The gel electrophoresis mobility shift assay is a technique for the qualitative and quantitative analysis of protein-DNA complexes. The ability to resolve reactants, reaction intermediates and products makes this method particularly well-suited for the measurement of the assembly and dissociation rates of protein-nucleic acid complexes. Here we identify conditions that must be met and variations of the technique that are useful for the measurement of reaction rates.
Gerstle
J T
JT
Department of Surgery, Pennsylvania State University College of Medicine, Hershey 17033.
Fried
M G
MG
D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007700:Kinetics; D007763:Lac Operon; D012097:Repressor Proteins / Q000378:metabolism
D002373:Cyclic AMP Receptor Protein; D012097:Repressor Proteins; D004247:DNA