title: Structure of the 16 S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF ('Mastigocladus laminosus HTF') strain PCC7518, and phylogenetic analysis.
abstract: The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were observed in the original isolate. Alternatively, contamination may have happened. To solve this problem, the 16 S rRNA sequence was determined and used to infer a secondary structure model and build distance trees. The trees showed that strain PCC7518 belongs to the cluster of heterocystous species and has most probably lost the ability to produce heterocysts by mutation. It is only distantly related to Chlorogloeopsis fritschii PCC6718.
authors.affiliation: Department Biochemie, Universiteit Antwerpen, (UIA), Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Wilmotte
authors.forename: G
authors.initials: G
authors.lastname: Van der Auwera
authors.forename: R
authors.initials: R
authors.lastname: De Wachter
mesh_terms: D001483:Base Sequence; D000458:Cyanobacteria / Q000145:classification* / Q000235:genetics; D004277:DNA, Single-Stranded; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D012336:RNA, Ribosomal, 16S