Major histocompatibility class II antigen expression on the surface of epidermal cells from normal and ultraviolet B irradiated subjects.
The influence of ultraviolet B irradiation in therapeutic doses on MHC II-positive epidermal cell numbers and their surface MHC II antigen expression was studied quantitatively using light microscopic immunoperoxidase and immunogold electron microscopic techniques. In multiple ultrathin sections through many MHC II-positive epidermal cells from five healthy subjects, prior to ultraviolet exposure, Langerhans cells and indeterminate cells were found to express similar densities of surface MHC II antigens, which were uniformly distributed over the cell surface. The variation in surface MHC II antigen expression on 97 dendritic epidermal cells from one subject was normally distributed. Following a 6-week course of ultraviolet B irradiation, in the same doses as those normally used for the treatment of psoriasis, MHC II-positive epidermal cell numbers were significantly reduced (mean decrease to 51% of the pre-UVB sample; p < 0.001 analysis of variance), but their surface MHC class II antigen density was significantly increased (p < 0.05 analysis of variance). No MHC II-negative Langerhans cells were detected in either the pre- or post-UVB samples.
Spencer
M J
MJ
University Department of Dermatology, Royal Infirmary of Edinburgh, Scotland, U.K.
Vestey
J P
JP
Tidman
M J
MJ
McVittie
E
E
Hunter
J A
JA
D000328:Adult; D001768:Blister / Q000209:etiology / Q000276:immunology / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology / Q000528:radiation effects*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013396:Suction; D014018:Tissue Distribution; D014466:Ultraviolet Rays
D000949:Histocompatibility Antigens Class II