title: Antibody-mediated enhancement of Legionella pneumophila-induced interleukin 1 activity.
abstract: The ability of antibody specific for Legionella pneumophila to enhance the induction of interleukin 1 (IL-1) production by murine peritoneal, splenic, and pulmonary macrophages in response to the bacterium was examined. Two preparations of L. pneumophila were utilized, a formalin-killed whole-cell preparation and viable bacteria. We measured both secreted (sIL-1) and cell membrane-associated (mIL-1) activities after incubation of the macrophages with the bacterial preparations in the presence or absence of the antibody. Both bacterial preparations induced sIL-1 and mIL-1 activities in each of the macrophage populations tested. These activities were generally enhanced by pretreating the bacteria with antibody, with the greatest enhancing activity observed for the formalin-killed preparations at lower doses of bacteria.
authors.affiliation: Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa 33612-4799.
authors.forename: R H
authors.initials: RH
authors.lastname: Widen
authors.forename: C A
authors.initials: CA
authors.lastname: Newton
authors.forename: T W
authors.initials: TW
authors.lastname: Klein
authors.forename: H
authors.initials: H
authors.lastname: Friedman
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000936:Antigen-Antibody Complex; D005260:Female; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000378:metabolism*; D016952:Legionella pneumophila / Q000276:immunology*; D016676:Macrophages, Alveolar / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009895:Opsonin Proteins; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen / Q000166:cytology
chemical_list: D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D007375:Interleukin-1; D009895:Opsonin Proteins