Cloning, characterization, and use in strain improvement of the Cephalosporium acremonium gene cefG encoding acetyl transferase.
A long open reading frame (ORF) closely linked to the Cephalosporium acremonium gene cefEF was identified by DNA sequencing. The cefEF gene encodes the enzyme involved in cephalosporin C (CPC) biosynthesis known as expandase/hydroxylase. Complementation of a C. acremonium cefG mutant, as well as expression of the gene in Aspergillus niger, showed this ORF to be the cefG gene, encoding cephalosporin C acetyltransferase, which catalyzes the last step in CPC biosynthesis. Analysis of transformants containing additional copies of this gene showed that a direct relationship exists between cefG copy number, cefG message levels, and CPC titers. This gene encodes an enzyme for what may be a rate-limiting step in CPC production.
Mathison
L
L
Panlabs, Inc. Bothell, WA 98011.
Soliday
C
C
Stepan
T
T
Aldrich
T
T
Rambosek
J
J
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000164:Acremonium / Q000235:genetics*; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000235:genetics; D001483:Base Sequence; D002511:Cephalosporins / Q000096:biosynthesis; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012331:RNA, Fungal / Q000235:genetics
D002511:Cephalosporins; D004271:DNA, Fungal; D012331:RNA, Fungal; C025163:cephalosporin C; D000123:Acetyltransferases; C018707:acetyl CoA-deacetylcephalosporin C acetyltransferase