Isolation of pigment-free bulk lipids from thylakoids.
Lipids from spinach thylakoids were extracted with chloroform/methanol and separated from pigments in a single chromatographic step run at 5 degrees C using silicic acid adjusted to pH 8. The isolated lipid fraction contained essentially the same amounts of individual lipids as in the initial extract. It contained less than 0.1% of the initial chlorophylls and carotenoids.
Bratt
C E
CE
Plant Biochemistry, University of Lund, Sweden.
Akerlund
H E
HE
D002845:Chromatography / Q000379:methods; D008055:Lipids / Q000302:isolation & purification*; D010860:Pigments, Biological / Q000302:isolation & purification; D010944:Plants / Q000737:chemistry*; D012824:Silicic Acid; D013696:Temperature
D008055:Lipids; D010860:Pigments, Biological; D012824:Silicic Acid