Investigation of the human metabolism of antipyrine using coupled liquid chromatography and nuclear magnetic resonance spectroscopy of urine.
The potential of coupled high-performance liquid chromatography-nuclear magnetic resonance spectroscopy for the detection and identification of drug metabolites has been investigated by direct analysis of human urine collected following administration of antipyrine. This approach provided a rapid method of characterizing the major human urinary metabolites of this drug and promises to be of widespread value in structural studies of xenobiotic metabolites.
Wilson
I D
ID
Department of Safety of Medicines, Zeneca Pharmaceuticals, Maccelesfield, Cheshire, UK.
Nicholson
J K
JK
Hofmann
M
M
Spraul
M
M
Lindon
J C
JC
D000983:Antipyrine / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet
D000983:Antipyrine