Immunoglobulin G subclass responses to mycobacterial lipoarabinomannan in HIV-infected and non-infected patients with tuberculosis.
Immunoglobulin G subclass responses to lipoarabinomannan (LAM) of Mycobacterium tuberculosis were determined by ELISA in both HIV-1 antibody positive (n = 31) and negative (n = 43) patients with tuberculosis (TB). Responses were also studied in a group of healthy controls (n = 16) and HIV-1 antibody positive (n = 60) individuals without TB. IgG2 antibodies were the predominant subclass, being present in 25 of 43 non-HIV-infected TB patients (58%) and in 11 of 31 HIV-infected TB patients (35%). However, HIV+ TB patients also showed IgG4 (n = 16; 52%), and IgG1 (n = 4, 13%) responses to LAM, whereas these subclasses were absent in sera from HIV-TB patients. Individuals in both non-tuberculous control groups showed no antibody responses to LAM. The influence of HIV infection on B cell responses to LAM, and possible mechanisms for antibody-mediated regulation of immunity to TB, are explored.
Da Costa
C T
CT
Department of Clinical Sciences, London School of Hygiene & Tropical Medicine, UK.
Khanolkar-Young
S
S
Elliott
A M
AM
Wasunna
K M
KM
McAdam
K P
KP
D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000145:classification; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D009161:Mycobacterium / Q000276:immunology*; D012737:Sex Factors; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000276:immunology*
D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; C050016:lipoarabinomannan