Characterisation of two identical independent non-homologous integration sites in mouse embryonic stem cells.
On analysis of 46 Geneticin-resistant (GtR) cell lines, derived by electroporation of mouse embryonic stem (ES) cells with a promoterless neo vector, we observed that in two independently derived cell lines, the vector had integrated into the same locus. The sequence flanking the vector integration site in both cell lines was cloned and sequenced. The vector had integrated into a 3 to 6-bp region in both cell lines. No homology is observed between the integration site sequence and the vector sequence.
Sutherland
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HF
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland.
Lovell-Badge
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RH
Jackson
I J
IJ
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA; D004351:Drug Resistance / Q000235:genetics; D018274:Electroporation; D004622:Embryo, Mammalian / Q000166:cytology; D005822:Genetic Vectors; D051379:Mice; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013234:Stem Cells / Q000378:metabolism*
D004247:DNA