Transforming growth factor beta exerts opposite effects from interleukin-1 beta on cultured rabbit articular chondrocytes through reduction of interleukin-1 receptor expression.
OBJECTIVE
The aim of the study was to determine whether transforming growth factor beta (TGF beta) modulates the effects and the receptor expression of interleukin-1 (IL-1) in rabbit articular chondrocytes (RAC).


METHODS
Collagen, glycosaminoglycan, and collagenase production, together with 125I-labeled IL-1 binding, were analyzed in RAC cultures.


RESULTS
TGF beta reduced both IL-1 effects on matrix metabolism and IL-1 receptor expression.


CONCLUSIONS
TGF beta acts as an antagonist of the effects of IL-1 through down-regulation of its receptor expression.
Rédini
F
F
Laboratoire de Biochimie du Tissu Conjonctif, CHU Côte de Nacre, Caen, France.
Mauviel
A
A
Pronost
S
S
Loyau
G
G
Pujol
J P
JP
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000648:ultrastructure; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D017364:Collagenases / Q000032:analysis; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology*; D011817:Rabbits; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D006025:Glycosaminoglycans; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D016212:Transforming Growth Factor beta; D003094:Collagen; D017364:Collagenases