Increased ADP-ribosylation of histones in oral cancer.
The involvement of ADP-ribosylation has been investigated by comparing the extent of ADP-ribosylation in two different stages of oral cancer with that of the corresponding normal oral tissue. Poly(ADP-ribose) synthetase(PADPRS), the enzyme that is responsible for this type of posttranslational covalent modification, was assayed first by measuring 14C-ADP-ribose incorporation into different acceptor proteins of the tissue homogenate. The results clearly indicate an increase in PADPRS activity during oral carcinogenesis. After observing this increased pattern in total tissue, further experiments were conducted to check the extent of ADP-ribosylation in purified nuclei. The data of this experiment also indicates a progressive increase in the extent of ADP-ribosylation with increase in malignancy of oral tissue. Further analysis of an ADP-ribosylation of chromosomal proteins indicates that the increased pattern of ADP-ribosylation is mainly attributed to histones and not to non-histone chromosomal proteins. Our investigation suggests that ADP-ribosylation may play a role in oral cancer and may be used as a potential tumour marker.
Das
B R
BR
Molecular Biology and Genetic Engineering Unit, Institute of Life Sciences, Orissa, India.
D000244:Adenosine Diphosphate / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009062:Mouth Neoplasms / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis
D006657:Histones; D000244:Adenosine Diphosphate; D011065:Poly(ADP-ribose) Polymerases