Preferential antibody targeting to small lymphoma metastases in the absence of the primary tumour.
Targeting of spontaneous liver metastases of the ESb.MP murine lymphoma was achieved with anti-CD2 monoclonal antibody (MAb) 12-15A, which does not react with normal liver tissue. Using quantitative autoradiography on whole body sections of animals that had received a standard dose of 1.1 MBq of 125I-labelled monoclonal antibody, metastases accumulated up to > 90% of the injected dose per gram (id/g). The average uptake of primary tumour lesions was at a low level of 24 Bq/mg (corresponding to 2.2% id/g) because of highly non-uniform accumulation, while metastatic lesions were all above 50 Bq/mg. Uptake was particularly pronounced in animals tested after resection of the primary tumour: 85% of metastases showed levels above 300 Bq/mg, which was the upper limit of uptake in metastases of non-resected animals. These findings demonstrate the potential of the antibody approach with regard to attacking residual metastatic lesions after debulking.
Schmid
U
U
Institut für Radiologie und Pathophysiologie, Krebsforschungszentrum, Heidelberg, F.R.G.
Schirrmacher
V
V
Momburg
F
F
Matzku
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D008223:Lymphoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008811:Mice, Inbred DBA
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes