Yeast coatomer contains a subunit homologous to mammalian beta'-COP.
The homologue of the mammalian coatomer complex was isolated from yeast cytosol and separated on a modified urea-containing SDS-polyacrylamide gel system. An additional band in the 100 kDa molecular weight range appeared when compared to the protein pattern obtained in conventional Laemmli gels, exactly as observed for mammalian coatomer. Cross-reactivity with an anti-peptide antibody raised against the C-terminus of beta'-COP from bovine, and N-terminal sequence analysis, revealed that this protein from yeast is related to beta'-COP from mammals.
Harter
C
C
Institute of Biochemistry I, University of Heidelberg, Germany.
Draken
E
E
Lottspeich
F
F
Wieland
F T
FT
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D020756:Coatomer Protein; D003429:Cross Reactions; D005656:Fungal Proteins / Q000737:chemistry*; D008869:Microtubule-Associated Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D000906:Antibodies; D020756:Coatomer Protein; D005656:Fungal Proteins; D008869:Microtubule-Associated Proteins; D010455:Peptides