title: Autoimmune Addison's disease. Analysis of autoantibody binding sites on human steroid 21-hydroxylase.
abstract: Human steroid 21-hydroxylase (21-OH) expressed in an in vitro translation system was found to react specifically with adrenal autoantibodies from patients with Addison's disease. The epitopes on 21-OH which reacted with autoantibodies were studied by incorporating a series of terminal and internal deletions into the 21-OH gene and analysing the expressed proteins by Western blotting. N-Terminal deletions up to amino acid 280 had no effect on autoantibody binding whereas a series of C-terminal deletions and truncations (amino acids 281-494) showed marked effects. Our results indicate that a central segment (281-379) and a C-terminal segment (380-494) of 21-OH interact to form at least one major autoantibody binding site.
authors.affiliation: Endocrine Immunology Unit, University of Wales College of Medicine, Heath Park, Cardiff, UK.
authors.forename: N
authors.initials: N
authors.lastname: Wedlock
authors.forename: T
authors.initials: T
authors.lastname: Asawa
authors.forename: A
authors.initials: A
authors.lastname: Baumann-Antczak
authors.forename: B R
authors.initials: BR
authors.lastname: Smith
authors.forename: J
authors.initials: J
authors.lastname: Furmaniak
mesh_terms: D000224:Addison Disease / Q000276:immunology*; D000328:Adult; D001323:Autoantibodies / Q000276:immunology*; D001483:Base Sequence; D001666:Binding Sites, Antibody; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015183:Restriction Mapping; D017384:Sequence Deletion; D013255:Steroid 21-Hydroxylase / Q000235:genetics / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D009838:Oligodeoxyribonucleotides; D013255:Steroid 21-Hydroxylase