Effect of adenosine 5'-triphosphate on secretagogue-stimulated (14C)-aminopyrine accumulation by rabbit isolated gastric glands.
The effect of adenosine 5'-triphosphate (ATP) on gastric acid secretion stimulated by histamine, carbachol and dibutyryl-cAMP (db-cAMP) was studied using glands isolated from rabbit gastric mucosa. The (14C)-aminopyrine (AP) accumulation method was used as an index of acid production by the gastric glands. Histamine-stimulated AP accumulation was significantly inhibited by ATP (10 mumol/l-1 mmol/l). The inhibitory action of ATP appeared to be specific, inasmuch as this nucleotide had no significant effect on basal secretion or secretion stimulated by carbachol or db-cAMP. The antisecretory effect of ATP on histamine-stimulated glands was not affected by the P1-purinoceptor antagonist, theophylline. Pretreatment of glands with indomethacin, a well known prostaglandin synthesis inhibitor, led to a significant reduction of the inhibitory responses to ATP. These results show that ATP inhibits the histamine-stimulated AP accumulation by rabbit isolated gastric glands and suggest that this effect is not due to an ectoenzymatic conversion of ATP into adenosine but to a direct effect of ATP which may be mediated via a P2-purinoceptor subtype linked to prostaglandin production.
Gil-Rodrigo
C E
CE
Departmento de Fisiología, Facultad de Medicina, Universidad del País Vasco (UPV-EHU), Bilbao, Spain.
Gomez
R
R
Gandarias
J M
JM
Galdiz
B
B
Carou
M
M
Bergaretxe
I
I
Vallejo
A
A
Ainz
L F
LF
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000632:Aminopyrine / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D010477:Perfusion; D011817:Rabbits; D013806:Theophylline / Q000494:pharmacology
D000632:Aminopyrine; D003994:Bucladesine; D006632:Histamine; D000255:Adenosine Triphosphate; D002217:Carbachol; D013806:Theophylline; D007213:Indomethacin