A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species.
Out of seeds of 4 Brassicaceae species, 7 antifungal proteins were isolated which are nearly identical to 2 previously characterized radish seed antifungal proteins. These basic proteins, multimers of a 5 kDa polypeptide, specifically inhibit fungal growth. One of the antifungal proteins has decreased antifungal activity and an increased antibacterial activity. In addition, the previously described antifungal activity of the radish seed 2S albumins was extended to the 2S albumins of the seeds of the 4 other Brassicaceae species. A 2S albumin-like trypsin-inhibitor from barley seeds was found to have much less activity against fungi.
Terras
F R
FR
F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Belgium.
Torrekens
S
S
Van Leuven
F
F
Osborn
R W
RW
Vanderleyden
J
J
Cammue
B P
BP
Broekaert
W F
WF
D000418:Albumins / Q000737:chemistry / Q000494:pharmacology; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification*; D003545:Cysteine / Q000737:chemistry; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry
D000418:Albumins; D000935:Antifungal Agents; D010940:Plant Proteins; D003545:Cysteine