Molecular cloning, functional expression and mRNA analysis of human beta-adrenergic receptor kinase 2.
In the present study the cDNA of human beta ARK2 was cloned using both PCR and cDNA library screening, subcloned into an expression vector and transiently expressed in COS7 cells. The expressed kinase activity was approximately 40% as efficient as human beta ARK1 in phosphorylating bovine rod outer segments in vitro. Northern blot analysis of human and bovine mRNA revealed a species-specific pattern of multiple hybridization bands, with two major transcripts in human rather than one in bovine. High levels of mRNA expression were found in peripheral blood leukocytes.
Parruti
G
G
Consorzio Mario Negri Sud, Istituto di Ricerche Farmacologiche Mario Negri, Santa Maria Imbaro, Italy.
Ambrosini
G
G
Sallese
M
M
De Blasi
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000378:metabolism; D019390:Arrestins; D015152:Blotting, Northern; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017868:Cyclic AMP-Dependent Protein Kinases; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012374:Rod Cell Outer Segment / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured; D051552:beta-Adrenergic Receptor Kinases; D000071557:beta-Arrestins
D000941:Antigens; D019390:Arrestins; D005136:Eye Proteins; D012333:RNA, Messenger; D000071557:beta-Arrestins; D004247:DNA; D011494:Protein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D051552:beta-Adrenergic Receptor Kinases