Induction of hair growth in ear wounds by cultured dermal papilla cells.
In the adult hair follicle the dermal papilla plays a crucial role in the dermal-epidermal interactions that control hair production and events of the growth cycle. It has previously been shown that cultured cells from rat vibrissa follicle dermal papillae can stimulate hair growth when implanted into amputated follicles. This study investigated the effects of implanting low-passage cultured papilla cells into small incisional wounds in the rat ear pinna. The groups of fibers that emerged from wound sites were much larger than local hairs, and often had vibrissa-type characteristics. Later-passage papilla cells or cultured skin fibroblasts failed to elicit the same response. Histology revealed that big follicles were formed when papilla cells were trapped between the cut edges of the epidermis. Abnormally large follicles were seen at wound sites many months post-operatively. Independent of epidermal influence, cultured papilla cells in the wound dermis formed rounded papilla-like aggregates that also persisted until biopsy. A previously described method of wrapping papilla cells in glabrous epidermis was less successful in percentage terms but resulted in the production of one very large vibrissa-type follicle and fiber. These results further illustrate that the inductive powers and developmental information retained by cultured dermal papilla cells parallel the properties of their embryonic precursors; the findings may have implications for human hair growth.
Jahoda
C A
CA
Department of Biological Sciences, University of Dundee, Scotland.
Reynolds
A J
AJ
Oliver
R F
RF
D000818:Animals; D002478:Cells, Cultured; D004423:Ear / Q000293:injuries* / Q000473:pathology; D004817:Epidermis / Q000502:physiology; D005260:Female; D006197:Hair / Q000254:growth & development*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012611:Scrotum; D012867:Skin / Q000166:cytology*; D012879:Skin Physiological Phenomena; D013997:Time Factors; D016378:Tissue Transplantation