Selective interaction of ferricyanide with cluster I of Clostridium pasteurianum 2[Fe4S4] ferredoxin.
Treatment of Clostridium pasteurianum ferredoxin (CpFd) with stoichiometric amounts of potassium ferricyanide results in the specific conversion of cluster I into a Fe3S4+ species while leaving cluster II unaltered. Ferricyanide-treated CpFd derivative has been purified and characterized through biochemical and spectroscopical techniques. The cluster conversion process is reversible and reconstitution of native CpFd has been afforded under appropriate conditions.
Bertini
I
I
Department of Chemistry, University of Florence, Italy.
Briganti
F
F
Calzolai
L
L
Messori
L
L
Scozzafava
A
A
D000595:Amino Acid Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D003013:Clostridium / Q000378:metabolism*; D003545:Cysteine; D005288:Ferredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008961:Models, Structural; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013455:Sulfur / Q000378:metabolism; D013816:Thermodynamics
D005288:Ferredoxins; D013455:Sulfur; D007501:Iron; D003545:Cysteine