Sample preparation for plasma measurement of plasminogen activator inhibitor-1 antigen in large population studies.
Plasminogen activator inhibitor-1 is important in regulating fibrinolysis and may be an important cardiovascular risk factor. Because of this, there is increased interest in performing plasminogen activator inhibitor-1 assays in large epidemiologic studies. Our aim in this study was to determine the simplest blood collection methods that yield accurate results with our plasminogen activator inhibitor-1 antigen assay. Our results indicate that the following issues are important: (1) since there is a large circadian variation in plasminogen activator inhibitor-1 plasma levels, a target time frame must be established; (2) commercially available citrate collection tubes are adequate, if sample processing is rapid; (3) careful venipuncture is necessary, with freely flowing collection; hemolysis must be avoided; and (4) centrifugation of at least 30,000 g.min is required to avoid platelet contamination.
Macy
E M
EM
Department of Pathology, University of Vermont, Burlington.
Meilahn
E N
EN
Declerck
P J
PJ
Tracy
R P
RP
D001800:Blood Specimen Collection / Q000379:methods; D002940:Circadian Rhythm; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood*; D012016:Reference Values; D014461:Ultracentrifugation / Q000379:methods
D017395:Plasminogen Activator Inhibitor 1