Translocation breakpoints are clustered on both chromosome 8 and chromosome 21 in the t(8;21) of acute myeloid leukemia.
The t(8;21)(q22;q22) is consistently associated with acute myeloid leukemia (AML) M2. Recent data have suggested that breakpoints on chromosome 21 are clustered within a single intron of a novel gene, AML1, just downstream of a region of homology to the runt gene of D melanogaster. In this report, we confirm rearrangement at the same location in at least 12 of 18 patients with t(8;21). Furthermore, we have isolated recombinant clones spanning the breakpoint regions on both the der(8) and the der(21) from one patient. By using a chromosome 8 probe derived from these clones, we show that t(8;21) breakpoints are also clustered on chromosome 8.
Tighe
J E
JE
Department of Haematology, Hammersmith Hospital, London, UK.
Daga
A
A
Calabi
F
F
D000208:Acute Disease; D000818:Animals; D015139:Blotting, Southern; D002891:Chromosomes, Human, Pair 21; D002898:Chromosomes, Human, Pair 8; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics; D005091:Exons; D015321:Gene Rearrangement; D015698:Genomic Library; D006801:Humans; D007438:Introns; D007951:Leukemia, Myeloid / Q000235:genetics*; D015183:Restriction Mapping; D014178:Translocation, Genetic
D015342:DNA Probes; D004273:DNA, Neoplasm