The structure of N-linked oligosaccharides of human pancreatic bile-salt-dependent lipase.
This study describes the structure of the N-linked oligosaccharide chains of bile-salt-dependent lipase isolated from the pancreatic juice of a normal donor. After hydrazinolysis, neutral sugar chains were separated from acidic chains by paper electrophoresis and were fractionated using serial column chromatography with immobilized lectins and Bio-Gel P-4 filtration. Structural analysis was performed by means of sequential glycosidase digestion and revealed that the neutral sugar chains are mainly of the biantennary complex type. Fucose residues were identified for some trimannosyl core structures and were alpha(1-6) or alpha(1-2) linked to the innermost GlcNAc residue and a terminal Gal residue, respectively. Sialyl residues were also involved in the oligosaccharide structures. Most of these structures have no linear N-acetyllactosamine repeats. Evidence from several approaches suggests that the sugar chains of the human pancreatic bile-salt-dependent lipase possess a blood-group-related antigenic determinant.
Sugo
T
T
Institute of Medical Science, University of Tokyo, Japan.
Mas
E
E
Abouakil
N
N
Endo
T
T
Escribano
M J
MJ
Kobata
A
A
Lombardo
D
D
D001647:Bile Acids and Salts / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006801:Humans; D008049:Lipase / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D010189:Pancreatic Juice / Q000201:enzymology*
D001647:Bile Acids and Salts; D009844:Oligosaccharides; D008049:Lipase