Molecular cloning of the smaller subunit(P52) of rat liver mitochondrial processing protease.
A cDNA encoding the smaller subunit (P52) of mitochondrial processing protease was isolated from a rat liver cDNA library, using cDNA fragment for yeast MAS1 as the probe. The deduced amino acid sequence is highly homologous to those of PEP from Neurospora crassa and MAS1 from Saccharomyces cerevisiae. After in vitro transcription and translation, the precursor peptide was imported into isolated rat liver mitochondria and processed to its mature form.
Kitada
S
S
Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka, Japan.
Niidome
T
T
Nagano
T
T
Ogishima
T
T
Ito
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics; D014176:Protein Biosynthesis; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D000096122:Mitochondrial Processing Peptidase
D003062:Codon; D004247:DNA; D046911:Macromolecular Substances; D008666:Metalloendopeptidases; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D000096122:Mitochondrial Processing Peptidase; C000715472:Mas1 protein, rat