CACC box and enhancer response of the human embryonic epsilon globin promoter.
The functional interaction between the human epsilon globin promoter and an erythroid-specific transcription enhancer, 5' HS-2, has been analyzed by transient expression assay. While stepwise deletion of DNA sequences between -852 and -122 had only small effects, removal of the CACC box at position -111 greatly decreased epsilon-globin promoter activity, as well as its response to the enhancer function of 5' HS-2 in erythroid cells. Our data demonstrated that the three ubiquitous promoter elements, the CACC, CCAAT, and TATA boxes, of the epsilon-globin-encoding gene together form a minimal promoter that would interact efficiently with 5' HS-2, and that at least the CACC box is an essential functional component of this enhancer-promoter interaction.
Motamed
K
K
Department of Genetics, University of California, Davis 95616.
Bastiani
C
C
Zhang
Q
Q
Bailey
A
A
Shen
C K
CK
D001483:Base Sequence; D004742:Enhancer Elements, Genetic / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D005914:Globins / Q000235:genetics*; D006367:HeLa Cells; D006412:Hematopoietic Stem Cells; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
D005914:Globins