title: Molecular characterization of the DNA puff C-8 gene of Rhynchosciara americana.
abstract: We have mapped the only transcription unit known to be present in the C-8 DNA puff of Rhynchosciara americana and describe the isolation and sequence of a cDNA clone, pRa C-8-22, which contains a nearly complete copy of the mRNA transcribed from this DNA puff and part of the sequence of genomic clone BSC8-0.9, which contains the promotor region and the remainder of the transcription unit. The characteristics of the protein predicted from the ORF present in the cDNA indicate that it is unique and secreted.
authors.affiliation: Departamento de Bioquímica, Universidade de S. Paulo, Brazil.
authors.forename: H M
authors.initials: HM
authors.lastname: Frydman
authors.forename: E O
authors.initials: EO
authors.lastname: Cadavid
authors.forename: J
authors.initials: J
authors.lastname: Yokosawa
authors.forename: F
authors.initials: F
authors.lastname: Henrique Silva
authors.forename: L D
authors.initials: LD
authors.lastname: Navarro-Cattapan
authors.forename: R V
authors.initials: RV
authors.lastname: Santelli
authors.forename: M
authors.initials: M
authors.lastname: Jacobs-Lorena
authors.forename: M
authors.initials: M
authors.lastname: Graessmann
authors.forename: A
authors.initials: A
authors.lastname: Graessmann
authors.forename: A J
authors.initials: AJ
authors.lastname: Stocker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D019476:Insect Proteins; D008969:Molecular Sequence Data; D012471:Salivary Proteins and Peptides / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C083747:C-8 protein, Rhynchosciara americana; D019476:Insect Proteins; D012471:Salivary Proteins and Peptides; D004247:DNA