Characterization and sequence analysis of rat serine:pyruvate/alanine:glyoxylate aminotransferase gene.
We have reported the isolation of genomic clones encoding serine:pyruvate aminotransferase (SPT; also named alanine:glyoxylate aminotransferase, AGT) (T. Oda, T. Funai, and A. Ichiyama, 1990, J. Biol. Chem. 265: 7513-7519). These clones contained the entire SPT/AGT gene of 10 kb. In this work, we characterized this gene. The SPT/AGT gene consists of 11 exons, and the exon-intron boundaries have typical splice donor and acceptor sequences. Determination of the nucleotide sequence up to -1.25 kb from the transcription initiation site revealed the presence of many putative cis elements, some of which may explain the transcriptional regulation of the SPT/AGT gene by glucagon and glucocorticoid. The nucleotide sequence around the 5' flanking region of the rat SPT/AGT gene and the whole gene organization were compared with those of the human SPT/AGT gene. No obvious similarities were observed in the 5' flanking region up to -1.25 kb from the initiation site of the gene, but exons 2 to 10 of the rat and human genes have identical sizes and show high similarities.
Oda
T
T
Department of Biochemistyr, Hamamatsu University School of Medicine, Japan.
Nishiyama
K
K
Ichiyama
A
A
D000410:Alanine Transaminase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004790:Enzyme Induction / Q000187:drug effects; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D005934:Glucagon / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007438:Introns; D008830:Microbodies / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D012326:RNA Splicing; D051381:Rats / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D000637:Transaminases / Q000235:genetics*; D014158:Transcription, Genetic
D005938:Glucocorticoids; D007328:Insulin; D005934:Glucagon; D000637:Transaminases; D000410:Alanine Transaminase; C019635:Alanine-glyoxylate transaminase; C022638:serine-pyruvate aminotransferase