Immunoregulatory functions of paf-acether. IX. Modulation of apoptosis in an immature T cell line.
We investigated the ability of a phospholipid cytokine, paf-acether to modulate programmed cell death in an immature human T cell line CEM-C12. Paf-acether alone did not cause cell death, but when it was added to CEM-C12 cells in the presence of calcium ionophore, a marked increase in cell mortality and DNA fragmentation was observed compared with calcium ionophore alone. This effect was dose-dependent between 2 and 10 microM paf-acether and specific in that lysophosphatidylcholine had a minimal effect. Thus, in association with another signal, paf modulates apoptotic processes in an immature human T cell line. This may be relevant to intrathymic lymphocyte maturation.
el Azzouzi
B
B
Institut National de la Santé et de la Recherche Medicale U200, Clamart, France.
Jurgens
P
P
Benveniste
J
J
Thomas
Y
Y
D017209:Apoptosis / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*
D008244:Lysophosphatidylcholines; D010972:Platelet Activating Factor; D000001:Calcimycin; D004247:DNA