Characterisation of dog allergens by means of immunoblotting.
Sera from 75 patients with clinical type I allergy against dogs were investigated by means of immunoblotting using extracts prepared from dog hair/dander (CAN XI D) and saliva. In addition, selected sera were tested on extracts made of hair, skin, salivary glands (parotis and submandibularis), serum and liver. A 69-kD IgE-binding protein was identified in all extracts tested with an incidence of approximately 40% and shown to be dog albumin by means of inhibition experiments. In 96% of patients' sera IgE antibodies reactive with a 19-kD and/or a 23-kD protein of the hair/dander extract (CAN XI D) were observed. IgE binding to a 23-kD band was also detected in the hair and saliva extracts, but not in skin, salivary gland, serum and liver extracts. A 19-kD IgE-binding protein was strongly expressed in skin and to a lesser degree in saliva, but not in hair, serum and liver. Preincubation of patients sera with the hair extract and subsequent probing with the hair/dander extract (CAN XI D) inhibited IgE binding to the 23 kD protein whereas preincubation with the skin extract abolished IgE binding to the 19-kD protein. Using the hair/dander extract as inhibitor, IgE binding to the 19- and 23-kD proteins of saliva was abrogated. Thus it is concluded that the 23-kD protein is preferentially expressed in hair and saliva whereas the 19-kD protein is found in saliva and skin. Furthermore these two proteins are likely to represent immunologically independent major allergens.
Spitzauer
S
S
Institute of Clinical Chemistry, University of Vienna, Austria.
Schweiger
C
C
Anrather
J
J
Ebner
C
C
Scheiner
O
O
Kraft
D
D
Rumpold
H
H
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000737:chemistry*; D000818:Animals; D000937:Antigen-Antibody Reactions; D002648:Child; D004285:Dogs / Q000276:immunology*; D005260:Female; D006197:Hair / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D008099:Liver / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011852:Radioallergosorbent Test; D012463:Saliva / Q000276:immunology; D012867:Skin / Q000276:immunology; D014020:Tissue Extracts / Q000276:immunology
D000485:Allergens; D014020:Tissue Extracts; D007073:Immunoglobulin E