Characterization of morphine-specific monoclonal antibodies showing minimal cross-reactivity with codeine.
Six murine monoclonal antibodies against morphine were produced using N-(4-aminobutyl)normorphine as a hapten. Most of the antibodies obtained distinguished the substituents at the 3 and 6 positions of morphine. This property of the antibodies led to a reduction in cross-reactivity with codeine, morphine-3-glucuronide (M-3-G) and morphine-6-glucuronide (M-6-G) to negligible levels. However, one of the antibodies distinguished the substituent only at the 3 position of morphine, which cross-reacted with M-6-G, naloxone and naltrexone. In the competitive inhibition enzyme-linked immunosorbent assay, morphine was detected at concentrations as low as circa 100 pg/ml.
Usagawa
T
T
Ube Laboratory, Ube Industries, Ltd., Japan.
Itoh
Y
Y
Hifumi
E
E
Takeyasu
A
A
Nakahara
Y
Y
Uda
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003061:Codeine / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009020:Morphine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D009020:Morphine; D003061:Codeine