Protection against cucumber mosaic virus (CMV) strains O and Y and chrysanthemum mild mottle virus in transgenic tobacco plants expressing CMV-O coat protein.
Transgenic tobacco expressing the coat protein (CP) of cucumber mosaic virus strain O (CMV-O) showed a significant level of protection against CMV strains O and Y. When inoculum concentrations were increased, the transformants showed a stronger level of protection against CMV-O than against CMV-Y. The substitution of an amino acid residue between CMV-O and CMV-Y, which is presumed to change the conformation of CP, may reflect the difference in susceptibility to these viruses. Furthermore the transgenic tobacco plants showed a significant level of protection against chrysanthemum mild mottle virus, a member of the cucumovirus group but with no serological relationship to CMV.
Nakajima
M
M
Plantech Research Institute, MKC Research Center, Yokohama, Japan.
Hayakawa
T
T
Nakamura
I
I
Suzuki
M
M
D002213:Capsid / Q000235:genetics / Q000276:immunology*; D009029:Mosaic Viruses / Q000276:immunology; D010935:Plant Diseases / Q000382:microbiology*; D010942:Plant Viruses / Q000276:immunology*; D030821:Plants, Genetically Modified / Q000276:immunology*; D010947:Plants, Toxic; D014026:Nicotiana / Q000276:immunology*