Comparison of structure and sequence of influenza B/Yamagata and B/Beijing neuraminidases shows a conserved "head" but much greater variability in the "stalk" and NB protein.
In 1988 the B/Yamagata/16/88 influenza virus appeared, which was antigenically quite different from the other B viruses circulating at that time. Neuraminidase heads of this strain were purified, crystallized, and analyzed by X-ray crystallography and compared with the structure of B/Beijing/1/87 neuraminidase. Only two point mutations could be detected in the "head" of the molecule leading to local rearrangement of amino acid side-chains. The nucleotide sequence, however, revealed significant differences in the stalk region and in the NB protein.
Burmeister
W P
WP
EMBL Grenoble Outstation, Grenoble, France.
Baudin
F
F
Cusack
S
S
Ruigrok
R W
RW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D014644:Genetic Variation; D009981:Influenza B virus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D009838:Oligodeoxyribonucleotides; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D014961:X-Ray Diffraction
D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D009439:Neuraminidase