Immunotherapy of tularemia: characterization of a monoclonal antibody reactive with Francisella tularensis.
An IgM monoclonal antibody (mAb) recognized surface antigens specific to Francisella tularensis wild-type (Schu4) and live vaccine strain (LVS), and reacted with both in ELISA and slide agglutination tests. This mAb also reacted with LVS microorganisms in tissues of infected mice as assessed by an indirect fluorescence technique. Western blot analysis showed the mAb to react with antigens associated with F. tularensis LPS.
Narayanan
R B
RB
Department of Cellular Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20850.
Drabick
J J
JJ
Williams
J C
JC
Fortier
A H
AH
Meltzer
M S
MS
Sadoff
J C
JC
Bolt
C R
CR
Nacy
C A
CA
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D001428:Bacterial Vaccines; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005604:Francisella tularensis / Q000276:immunology* / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000627:therapeutic use; D007167:Immunotherapy; D008099:Liver / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D014406:Tularemia / Q000276:immunology / Q000473:pathology / Q000628:therapy*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007075:Immunoglobulin M