Membrane-incorporated surrogate receptors increase antigen presentation by resting B cells, but not by LPS-activated blasts.
Palmitate-conjugated monoclonal antibodies specific to ovalbumin were inserted into the cell membrane of normal resting B cells and LPS-activated blasts. These two decorated B cells were tested for their ability to act as antigen-presenting cells for ovalbumin-specific I-Ad-restricted T-cell hybridomas. It was found that the antibody-decorated resting B cells presented antigen more efficiently than non-decorated controls. However, no increment was observed when decorated LPS blasts were compared with non-decorated blasts. This is explained by the fact that the inserted antibodies quickly disappeared from the cell membrane of LPS blasts, while they were retained for a long period in the membrane of resting B cells.
Gontijo
C M
CM
Department of Biochemistry and Immunology, Federal University of Minas Gerais, Brazil.
Möller
G
G
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011971:Receptors, Immunologic / Q000502:physiology*
D008070:Lipopolysaccharides; D011971:Receptors, Immunologic