Chromosomal localization and genomic cloning of the mouse alpha-tropomyosin gene Tpm-1.
In vertebrates, the alpha-tropomyosin gene, Tpm-1, codes for at least 9 tropomyosin isoforms that are expressed by tissue-specific alternative splicing. Using interspecies backcrosses, we have localized Tpm-1 on mouse chromosome 9, cen-Cyp1a2-Tpm-1-Mod-1-Mylc-Scn5a, near the d-se region. The restriction fragment length variant used for chromosomal assignment, as well as other restriction fragments hybridizing to a 3'-specific alpha-tropomyosin cDNA probe in genomic Southern blots, was investigated by cloning 17.5-kb of Tpm-1. The same restriction patterns were observed, proving the identity of the mapped and the cloned gene. The identity was supported by sequencing the 3' end of the gene.
Schleef
M
M
Developmental Biology Unit, University of Bielefeld, Federal Republic of Germany.
Werner
K
K
Satzger
U
U
Kaupmann
K
K
Jockusch
H
H
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D015342:DNA Probes; D005091:Exons; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014335:Tropomyosin / Q000235:genetics*
D015342:DNA Probes; D005819:Genetic Markers; D014335:Tropomyosin