Adsorption/desorption of human serum albumin at the surface of poly(lactic acid) nanoparticles prepared by a solvent evaporation process.
Biocompatible and biodegradable nanoparticles of poly(lactic acid) (100% L-lactic units = PLA) were prepared by an emulsion, microfluidization, and solvent evaporation method using human serum albumin (HSA) as a surface agent. A radiolabeling technique was employed to quantify the serum albumin bound to the nanoparticles and to measure its desorption kinetics in various media at 22 degrees C and 37 degrees C (phosphate buffer pH 7.4, serum albumin 40 g/L in phosphate buffer pH 7.4 and fetal calf serum). The amount of serum albumin bound to the nanoparticles was found to be a linear function of 1/D (where D is the nanoparticle mean diameter) and was related to the total developed area of the nanoparticles. The adsorption/desorption behavior of serum albumin at the surface of the nanoparticles suggested a multilayer adsorption model. Moreover, a part of the serum albumin molecules was irreversibly bound regardless of the incubation conditions. Consequently, the classical Langmuirian theories of equilibria could not be applied.
Verrecchia
T
T
Rhone-Poulenc Rorer, IBP-PHTEC, New Drug Delivery System Unit, Antony, France.
Huve
P
P
Bazile
D
D
Veillard
M
M
Spenlehauer
G
G
Couvreur
P
P
D000327:Adsorption; D001672:Biocompatible Materials; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000737:chemistry; D019344:Lactic Acid; D008422:Materials Testing; D008854:Microscopy, Electron; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry / Q000493:pharmacokinetics*; D012997:Solvents; D013499:Surface Properties; D013696:Temperature
D001672:Biocompatible Materials; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D012709:Serum Albumin; D012997:Solvents; D019344:Lactic Acid; C033616:poly(lactide)