title: Demonstration of acrosomal membranes using the hypo-osmotic swelling test.
abstract: The state of the acrosomal membranes in human spermatozoa was studied by means of the hypo-osmotic swelling test and indirect immunofluorescence using anti-boar outer acrosomal membrane antibodies. The swelling phenomenon observed in the acrosomal region was characterized by expansion of the plasma membrane without modification of the outer acrosomal membrane.
authors.affiliation: Department of Preclinical Sciences, University of La Frontera, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Sanchez
authors.forename: M
authors.initials: M
authors.lastname: Concha
authors.forename: E
authors.initials: E
authors.lastname: Töpfer-Petersen
authors.forename: W B
authors.initials: WB
authors.lastname: Schill
mesh_terms: D000177:Acrosome / Q000502:physiology / Q000648:ultrastructure*; D002462:Cell Membrane / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007038:Hypotonic Solutions; D008297:Male; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D009997:Osmotic Pressure
chemical_list: D007038:Hypotonic Solutions