A genetic region downstream of the hydrogenase structural genes of Bradyrhizobium japonicum that is required for hydrogenase processing.
Deletion of a 2.9-kb chromosomal EcoRI fragment of DNA located 2.2 kb downstream from the end of the hydrogenase structural genes resulted in the complete loss of hydrogenase activity. The normal 65- and 35-kDa hydrogenase subunits were absent in the deletion mutants. Instead, two peptides of 66.5 and 41 kDa were identified in the mutants by use of anti-hydrogenase subunit-specific antibody. A hydrogenase structural gene mutant did not synthesize either the normal hydrogenase subunits or the larger peptides. Hydrogenase activity in the deletion mutants was complemented to near wild-type levels by plasmid pCF1, containing a 6.5-kb BglII fragment, and the 65- and 35-kDa hydrogenase subunits were also recovered in the mutants containing pCF1.
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Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
Maier
R J
RJ
D003360:Cosmids / Q000235:genetics; D003429:Cross Reactions; D004794:Enzyme Repression / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D006864:Hydrogenase / Q000235:genetics* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009532:Nickel / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D012230:Rhizobiaceae / Q000201:enzymology / Q000235:genetics*
C022838:nickel chloride; D009532:Nickel; D006864:Hydrogenase