Progestational regulation of human endometrial stromal cell tissue factor expression during decidualization.
Decidualized endometrial stromal cells from mid- to late secretory phase and decidual cells from gestational human endometrium displayed prominent immunohistochemical staining for tissue factor, a primary initiator of hemostasis. Consistent with the regulation by progesterone of the decidualization process in vivo, medroxy-progesterone acetate elevated the tissue factor content of primary stromal cell cultures 8-fold over basal values. This increase paralleled the release of immunoreactive PRL, a marker of decidualization. The induced, as well as basal, tissue factor displayed full functional activity and the expected electrophoretic mobility (46 kilodaltons). Moreover, Northern analysis of RNA from cultured stromal cells indicated that medroxyprogesterone acetate increased tissue factor mRNA levels approximately 10-fold relative to control levels. In contrast, cultured stromal cell tissue factor protein content and mRNA levels were unaffected by exogenous estradiol. These findings indicate that enhancement of endometrial stromal cell tissue factor content is associated with progesterone induction of the decidualization process. In humans, trophoblastic invasion of the endometrial vasculature during blastocyst implantation risks hemorrhage. Therefore, increases in perivascular decidual cell tissue factor expression could serve to promote periimplantational endometrial hemostasis.
Lockwood
C J
CJ
Department of Obstetrics, Gynecology and Reproductive Sciences, Mount Siani School of Medicine, New York, New York 10029.
Nemerson
Y
Y
Guller
S
S
Krikun
G
G
Alvarez
M
M
Hausknecht
V
V
Gurpide
E
E
Schatz
F
F
D015152:Blotting, Northern; D002478:Cells, Cultured; D015342:DNA Probes; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology*; D008597:Menstrual Cycle; D011388:Prolactin / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013925:Thromboplastin / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D015342:DNA Probes; D012333:RNA, Messenger; D004958:Estradiol; D011388:Prolactin; D013925:Thromboplastin; D017258:Medroxyprogesterone Acetate