title: Nucleotide and deduced amino acid sequence of porcine interleukin 4 cDNA derived from lamina propria lymphocytes.
abstract: Total RNA was isolated from in vitro activated lamina propria lymphocytes and used to direct the synthesis of cDNA. Interleukin 4 transcripts were then specifically amplified by PCR. Comparison of the nucleotide sequence with its human homologue demonstrates deletion within the coding region of pig interleukin 4 centred around amino acid residue 70 in the mature human protein.
authors.affiliation: Department of Veterinary Medicine, University of Bristol, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bailey
authors.forename: A C
authors.initials: AC
authors.lastname: Perry
authors.forename: P W
authors.initials: PW
authors.lastname: Bland
authors.forename: C R
authors.initials: CR
authors.lastname: Stokes
authors.forename: L
authors.initials: L
authors.lastname: Hall
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D016415:Sequence Alignment; D013045:Species Specificity; D013552:Swine
chemical_list: D012333:RNA, Messenger; D015847:Interleukin-4; D004247:DNA