Rate and mechanism of nonhomologous recombination during a single cycle of retroviral replication.
Oncogenes discovered in retroviruses such as Rous sarcoma virus were generated by transduction of cellular proto-oncogenes into the viral genome. Several different kinds of junctions between the viral and proto-oncogene sequences have been found in different viruses. A system of retrovirus vectors and a protocol that mimicked this transduction during a single cycle of retrovirus replication was developed. The transduction involved the formation of a chimeric viral-cellular RNA, strand switching of the reverse transcription growing point from an infectious retrovirus to the chimeric RNA, and often a subsequent deletion during the rest of viral DNA synthesis. A short region of sequence identity was frequently used for the strand switching. The rate of this process was about 0.1 to 1 percent of the rate of homologous retroviral recombination.
Zhang
J
J
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
Temin
H M
HM
D000818:Animals; D001483:Base Sequence; D002934:Cinnamates; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D006921:Hygromycin B / Q000031:analogs & derivatives; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics; D009355:Neomycin; D010957:Plasmids; D011519:Proto-Oncogenes; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D014779:Virus Replication
D002934:Cinnamates; D004279:DNA, Viral; D012367:RNA, Viral; D006921:Hygromycin B; C026273:hygromycin A; D009355:Neomycin