Evidence for separate Na+, K+, Cl- and K+, Cl- co-transport systems in mouse pancreatic beta-cells.
The effects of H 25 and H 74 on ouabain-resistant 86Rb+ influx were tested in beta-cell-rich mouse pancreatic islets. Both H 25, which is considered to be a specific inhibitor of Na+, K+, Cl- co-transport, and H 74, a specific inhibitor of K+, Cl- co-transport, reduced the ouabain-resistant 86Rb+ influx. The specific inhibitory effects of H 25 and H 74 on 86Rb+ influx did not overlap. The data suggest that mouse pancreatic beta-cells are equipped with separate systems for Na+, K+, Cl- and K+, Cl- co-transport.
Sandström
P E
PE
Department of Histology and Cell Biology, University of Umeå, Sweden.
Sehlin
J
J
D000097:Acetonitriles / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism*; D002713:Chlorine / Q000378:metabolism*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008820:Mice, Obese; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters; D013424:Sulfanilamides / Q000494:pharmacology; D027981:Symporters; D000096922:K Cl- Cotransporters
D000097:Acetonitriles; D002352:Carrier Proteins; D002713:Chlorine; D010042:Ouabain; D011188:Potassium; D012964:Sodium; D028021:Sodium-Potassium-Chloride Symporters; D013424:Sulfanilamides; D027981:Symporters; D000096922:K Cl- Cotransporters; C039438:benzmetanide; C039274:H 74