Inhibition kinetics of brain butyrylcholinesterase by Cd2+ and Zn2+, Ca2+ or Mg2+ reactivates the inhibited enzyme.
1. The inhibition kinetics of sheep brain butyrylcholinesterase (BChE) (acylcholine acylhydrolase, EC 3.1.1.8) by Cd2+ and Zn2+ has been studied. 2. KS has been determined as 0.14 mM. Cd2+ and Zn2+ were the hyperbolic mixed-type inhibitors of BChE. Ca2+ and Mg2+ had no effect on the enzyme activity in the experimental conditions. 3. But when the enzyme was inhibited by 0.1 mM Cd2+ or Zn2+, Ca2+ and Mg2+ reactivated the inhibited form of BChE.
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Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
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D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002104:Cadmium / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D012756:Sheep; D015032:Zinc / Q000037:antagonists & inhibitors / Q000494:pharmacology
D002413:Cations, Divalent; D002800:Cholinesterase Inhibitors; D008670:Metals; D002104:Cadmium; D008274:Magnesium; D015032:Zinc; D002118:Calcium