Elastomeric polytetrapeptide matrices: hydrophobicity dependence of cell attachment from adhesive (GGIP)n to nonadhesive (GGAP)n even in serum.
The cross-linked polytetrapeptide matrices based on the repeating amino acid sequences, GGAP, GGVP and GGIP, were prepared and tested for cell adhesion promoting activity in both the absence and presence of fetal bovine serum. For comparison, X20-poly(GVGVP), a matrix previously shown to be a poor support for cell attachment and spreading, was included. In the absence of serum, all three polytetrapeptide-based matrices and the polypentapeptide-based matrix were negative for the adhesion of fibroblasts and endothelial cells. In the presence of serum, various sub-maximal levels of cell adhesion were found for all matrices except for the matrix based on GGAP. An apparent correlation was noted between the degree of cell attachment to the different polytetrapeptide-based matrices and the hydrophobicity of those matrices where increased hydrophobicity results in increased cell attachment. The property of being refractory to ligamentum nuchae fibroblast and human umbilical vein endothelial cell adhesion in the presence of serum indicates a potential use for X20-poly(GGAP) in the development of, for example, additional physical barriers for the prevention of post-surgical and post-trauma adhesions.
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Laboratory of Molecular Biophysics, School of Medicine, University of Alabama at Birmingham 35294-0019.
Gowda
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Parker
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Urry
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DW
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003432:Cross-Linking Reagents; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D005347:Fibroblasts; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000097:blood / Q000737:chemistry*; D010455:Peptides / Q000097:blood / Q000737:chemistry*; D011247:Pregnancy; D014471:Umbilical Veins / Q000166:cytology
D003432:Cross-Linking Reagents; D009842:Oligopeptides; D010455:Peptides