Lack of role of TGF-beta 1 in decreased lymphoproliferative response in HIV-1 infection.
We investigated whether reduction of the phytohemagglutinin (PHA)-induced proliferative response of lymphocytes from HIV type-1 (HIV-1)-infected (HIV+) individuals could be explained by overproduction of transforming growth factor-beta 1 (TGF-beta 1), a strong inhibitor of T cell proliferation. PHA-stimulated PBMC from 40 HIV- and 42 HIV+ homosexual men from the Baltimore Center of the Multicenter AIDS Cohort Study (MACS) were studied using Northern blot analysis of expression of TGF-beta 1 mRNA and determining the effects of anti-TGF-beta 1 neutralizing antibody on PHA-induced proliferative responses. Compared to the HIV- donors, HIV+ donors did not show increased expression of TGF-beta 1 mRNA in unstimulated or PHA-stimulated PBMC. Furthermore, a neutralizing antibody to TGF-beta 1 did not reverse the decreased proliferative response of PBMC from HIV+ individuals to PHA or interleukin-2. These results indicate that TGF-beta 1 is not involved in T cell proliferation defects seen in HIV+ donors.
Chopra
R K
RK
Department of Environmental Health Sciences, School of Hygiene and Public Health, Baltimore, Maryland 21205.
Raj
N B
NB
Scally
J P
JP
Cappuccio
W R
WR
Nagel
J E
JE
Saah
A J
AJ
Margolick
J B
JB
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000503:physiopathology*; D000906:Antibodies / Q000494:pharmacology; D015870:Gene Expression / Q000235:genetics; D006679:HIV Seropositivity / Q000235:genetics / Q000503:physiopathology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*
D000906:Antibodies; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta