Bombesin and neurotensin excite neurons in hypothalamic arcuate nucleus in brain slices: an extracellular single-unit study.
Using extracellular single-unit recording in brain tissue slices, the effects of bombesin (Bomb), neurotensin (NT), and their analogs on hypothalamic arcuate (ARC) neurons were tested in this study. Adult ovariectomized Sprague-Dawley rats were used for obtaining the brain slices. Both Bomb and NT in pmol ranges stimulated nearly 70% of ARC neurons tested and the effects were all very significant. [D-Trp11]-NT, a NT analog, behaved more like an analog of NT instead of an antagonist, although [Leu13-psi(CH2NH)-Leu14]-Bomb, a Bomb receptor antagonist, could not block most of Bomb's effects on ARC neurons. The significant effects of Bomb and NT shown in this report indicate that both peptides may play significant roles in the neuroendocrine control system.
Lin
J Y
JY
Institute of Physiology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Pan
J T
JT
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000502:physiology*; D001839:Bombesin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004594:Electrophysiology; D005071:Evoked Potentials / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009496:Neurotensin / Q000031:analogs & derivatives / Q000494:pharmacology*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley
C057644:bombesin, Leu(13)-psi-CH2NH-Leu(14)-; D009496:Neurotensin; C024309:neurotensin, Trp(11)-; D001839:Bombesin