Demonstration of acrosomal membranes using the hypo-osmotic swelling test.
The state of the acrosomal membranes in human spermatozoa was studied by means of the hypo-osmotic swelling test and indirect immunofluorescence using anti-boar outer acrosomal membrane antibodies. The swelling phenomenon observed in the acrosomal region was characterized by expansion of the plasma membrane without modification of the outer acrosomal membrane.
Sanchez
R
R
Department of Preclinical Sciences, University of La Frontera, Chile.
Concha
M
M
Töpfer-Petersen
E
E
Schill
W B
WB
D000177:Acrosome / Q000502:physiology / Q000648:ultrastructure*; D002462:Cell Membrane / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007038:Hypotonic Solutions; D008297:Male; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D009997:Osmotic Pressure
D007038:Hypotonic Solutions