Expression of equine herpesvirus 1 glycoprotein D by using a recombinant baculovirus.
Glycoprotein D (gD) of equine herpesvirus 1 (EHV-1) was expressed at the surface of insect cells infected by a recombinant baculovirus. EHV-1 gD was detected as multiple forms (56, 52, and 48 kDa) from 18 to 96 h postinfection. Laboratory animals inoculated with the recombinant EHV-1 gD developed neutralizing antibody responses against both EHV-1 and EHV-4.
Love
D N
DN
Department of Veterinary Pathology, University of Sydney, New South Wales, Australia.
Bell
C W
CW
Pye
D
D
Edwards
S
S
Hayden
M
M
Lawrence
G L
GL
Boyle
D
D
Pye
T
T
Whalley
J M
JM
D000818:Animals; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000276:immunology; D009036:Moths; D009500:Neutralization Tests; D017924:Nucleopolyhedroviruses / Q000235:genetics; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1