title: Saliva-binding protein (SsaB) from Streptococcus sanguis 12 is a lipoprotein.
abstract: Two lipoprotein consensus sequences (Leu-X-X-Cys) are found in the presumptive signal peptide region (positions 12 to 15 and 17 to 20) of saliva-binding protein (SsaB) from Streptococcus sanguis 12. Three analogs of SsaB containing Cys-->Gly mutations were constructed by site-directed mutagenesis of pSA2, the recombinant plasmid expressing SsaB. [3H]palmitate was incorporated into SsaB only when the native Cys-20 residue was present. These data show that SsaB is a lipoprotein and that Cys-20 is the critical site for acylation.
authors.affiliation: Laboratory of Microbial Ecology, National Institute of Dental Research, Bethesda, Maryland 20892.
authors.forename: N
authors.initials: N
authors.lastname: Ganeshkumar
authors.forename: N
authors.initials: N
authors.lastname: Arora
authors.forename: P E
authors.initials: PE
authors.lastname: Kolenbrander
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008074:Lipoproteins / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification; D015183:Restriction Mapping; D012463:Saliva; D013298:Streptococcus sanguis / Q000235:genetics* / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D008074:Lipoproteins; D010169:Palmitic Acids; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C067560:saliva-binding protein, Streptococcus sanguis 12; D019308:Palmitic Acid