Assembly of prothrombinase complex.
The approaches described in this article have resulted in an increased understanding of the reaction steps involved in the stabilization and assembly of the prothrombinase complex. Because prothrombinase is considered an archetype for some of the other coagulation complexes, the quantitative information derived from these studies (Table I) provides the framework for future studies of prothrombinase and suggests experimental approaches for studies of the other analogous coagulation reactions.
Krishnaswamy
S
S
Department of Medicine, Emory University, Atlanta, Georgia 30322.
Nesheim
M E
ME
Pryzdial
E L
EL
Mann
K G
KG
D000818:Animals; D002151:Calorimetry; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D005165:Factor V / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D015943:Factor Va / Q000302:isolation & purification / Q000378:metabolism; D005170:Factor X / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D015951:Factor Xa / Q000302:isolation & purification / Q000378:metabolism; D005454:Fluorescence Polarization / Q000379:methods; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007700:Kinetics; D008433:Mathematics; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; C022475:prothrombinase complex; D015943:Factor Va; D005165:Factor V; D005170:Factor X; D015951:Factor Xa