High-resolution comparative mapping of mouse chromosome 17.
We have constructed a high-resolution genetic linkage map spanning the proximal 32 cM of mouse chromosome 17 including the t complex. Simple sequence repeats at D17Tu1 and D17Mit6 were employed to identify 121 recombinants among 374 offspring of a (C57BL/6 x CAST/Ei) x C57BL/6 backcross. In contrast to previously reported interspecific Mus domesticus x Mus spretus backcrosses, we did not observe inversion polymorphisms with our cross. This and the relatively high frequency of DNA polymorphisms between C57BL/6 and CAST/Ei allowed us to put 32 RFLV markers and 32 PCR markers on a single map. We present the localization of four new DNA markers and determined map positions for 10 other loci, which previously had been assigned to intervals of the t complex only through the study of partial t haplotype chromosomes.
Himmelbauer
H
H
Department of Molecular Biology, Princeton University, New Jersey 08544-1014.
Silver
L M
LM
D000818:Animals; D001483:Base Sequence; D007446:Chromosome Inversion; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D003433:Crosses, Genetic; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D006824:Hybridization, Genetic; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D015342:DNA Probes; D005819:Genetic Markers