title: Generation of a new adenovirus type 12-inducible fragile site by insertion of an artificial U2 locus in the human genome.
abstract: Infection with adenovirus type 12 (Ad12) induces four fragile sites in the human genome (H.F. Stich, G.L. van Hoosier, and J.J. Trentin, Exp. Cell Res. 34:400-403, 1964; H. zur Hausen, J. Virol. 1:1174-1185, 1967). The major site, at 17q21-22, contains the U2 gene cluster, which is specifically disrupted by infection in at least a percentage of the cells (D.M. Durnam, J.C. Menninger, S.H. Chandler, P.P. Smith, and J.K. McDougall, Mol. Cell. Biol. 8:1863-1867, 1988). For direct assessment of whether the U2 locus is the target of the Ad12 effect, an artificial locus, constructed in vitro and consisting of tandem arrays of the U2 6-kbp monomer, was transfected into human cells. We report that integration of this artificial locus on the p arm of chromosome 13 creates a new Ad12-inducible fragile site.
authors.affiliation: Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Y P
authors.initials: YP
authors.lastname: Li
authors.forename: R
authors.initials: R
authors.lastname: Tomanin
authors.forename: J R
authors.initials: JR
authors.lastname: Smiley
authors.forename: S
authors.initials: S
authors.lastname: Bacchetti
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D015139:Blotting, Southern; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D004249:DNA Damage; D005813:Genes, Synthetic; D015894:Genome, Human; D006801:Humans; D008856:Microscopy, Fluorescence; D005810:Multigene Family; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012342:RNA, Small Nuclear / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D016662:Virus Integration / Q000502:physiology
chemical_list: D012341:RNA, Ribosomal, 5S; D012342:RNA, Small Nuclear