title: Tie-1 and tie-2 define another class of putative receptor tyrosine kinase genes expressed in early embryonic vascular system.
abstract: We report the molecular cloning and characterization of two structurally related putative receptor tyrosine kinases, encoded by distinct genes (tie-1 and tie-2) on mouse chromosome 4. Both tie-1 and tie-2 encode receptor proteins possessing unique multiple extracellular domains: two immunoglobulin-like loop domains flanking three epidermal growth factor repeats followed by three fibronectin-type III repeats. Both genes are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. tie-1, but not tie-2, is expressed in an acute myelogenic cell line in vitro. tie-1 and tie-2 may form another class within the receptor tyrosine kinase gene family, and further characterization of these genes and identification of their putative ligands should define the nature of the signal-transduction cascades underlying early vascular system development, as well as their differential roles in mesodermal cells of the amniotic and myeloid lineages.
authors.affiliation: Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
authors.forename: T N
authors.initials: TN
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.forename: C A
authors.initials: CA
authors.lastname: Kozak
authors.forename: K L
authors.initials: KL
authors.lastname: Audus
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000502:physiology; D015870:Gene Expression; D005796:Genes; D008040:Genetic Linkage; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D020794:Receptor Protein-Tyrosine Kinases