Rapid and highly sensitive high-performance liquid chromatographic method for the determination of histamine and 3-methylhistamine in biological samples using fluorescamine as the derivatizing agent.
A highly sensitive and rapid high-performance liquid chromatographic assay for the determination of histamine and 3-methylhistamine in biological samples using 1-methylhistamine as the internal standard is described. Samples were purified and concentrated on cation-exchange columns and derivatized with fluorescamine. The lower detection limit was 20 pg on-column. Linearity was demonstrated up to 20 ng on-column. The samples could be derivatized simultaneously before injection and were stable for 7 days. The method was used for the determination of histamine and related compounds in coronary perfusates, extracts of homogenized rat hearts, and supernatants of stimulated peritoneal mast cells.
van Haaster
C M
CM
Cardiovascular Research Institute Maastricht, University of Limburg, Netherlands.
Engels
W
W
Lemmens
P J
PJ
Hornstra
G
G
van der Vusse
G J
GJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005450:Fluorescamine / Q000737:chemistry*; D006632:Histamine / Q000032:analysis*; D008297:Male; D008407:Mast Cells / Q000737:chemistry; D008761:Methylhistamines / Q000032:analysis*; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D008761:Methylhistamines; D005450:Fluorescamine; C031205:3-methylhistamine; D006632:Histamine