Site-directed mutagenesis and expression of coagulation factors VIII and V in mammalian cells.
Pittman
D D
DD
Genetics Institute, Inc., Cambridge, Massachusetts 02140.
Kaufman
R J
RJ
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D017077:Culture Media, Conditioned; D018274:Electroporation / Q000379:methods; D005165:Factor V / Q000096:biosynthesis* / Q000235:genetics; D005169:Factor VIII / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007202:Indicators and Reagents; D008715:Methionine / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D013462:Sulfur Radioisotopes; D014162:Transfection / Q000379:methods*
D017077:Culture Media, Conditioned; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D005165:Factor V; D005169:Factor VIII; D008715:Methionine