title: Usefulness of nucleolar organizer region staining in gastric myogenic tumors: correlation with ploidy by DNA flow cytometry.
abstract: A silver-staining technique to identify nucleolar organizer region-associated proteins (Ag-NOR) was applied to 55 gastric myogenic tumors. The mean numbers of Ag-NOR in the nucleus were: leiomyoma (30 cases), 2.0 +/- 0.6 (mean +/- SD); low-grade leiomyosarcoma (11 cases), 3.0 +/- 0.7; and high-grade leiomyosarcoma (14 cases), 3.9 +/- 0.7. The Ag-NOR counts were compared with DNA ploidy as determined by flow cytometry in 46 tumors. The Ag-NOR counts were significantly different in the aneuploid leiomyosarcomas (4.1 +/- 0.6: mean +/- SD) and diploid leiomyosarcomas (2.9 +/- 0.6: mean +/- SD). Thus, this parameter may serve as an objective histological discriminant for malignancy.
authors.affiliation: First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shimamoto
authors.forename: K
authors.initials: K
authors.lastname: Haruma
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Todo
authors.forename: M
authors.initials: M
authors.lastname: Yoshihara
authors.forename: K
authors.initials: K
authors.lastname: Sumii
authors.forename: G
authors.initials: G
authors.lastname: Kajiyama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007889:Leiomyoma / Q000737:chemistry* / Q000235:genetics; D007890:Leiomyosarcoma / Q000737:chemistry* / Q000235:genetics; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000737:chemistry*; D011003:Ploidies; D016622:Silver Staining; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000235:genetics
chemical_list: D034961:Antigens, Nuclear; D004273:DNA, Neoplasm; D009687:Nuclear Proteins; C051963:nucleolar organizer region associated proteins