Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA.
The endogenous production of oxidative damage in DNA by free radicals released as a by-product of respiration is a likely cause of mutations which, if they occur in appropriate genes, may lead to cancer. Using an endonuclease specific for oxidized pyrimidines, in conjunction with the highly sensitive method of single cell gel electrophoresis, we have detected significant oxidative damage in untreated, freshly isolated lymphocytes from normal, healthy individuals.
Collins
A R
AR
Rowett Research Institute, Bucksburn, Aberdeen, UK.
Duthie
S J
SJ
Dobson
V L
VL
D004249:DNA Damage; D004278:DNA, Superhelical / Q000032:analysis* / Q000187:drug effects; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004706:Endodeoxyribonucleases; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008214:Lymphocytes / Q000737:chemistry*; D010084:Oxidation-Reduction
D004278:DNA, Superhelical; D006861:Hydrogen Peroxide; D004706:Endodeoxyribonucleases