title: The adenovirus protease is activated by a virus-coded disulphide-linked peptide.
abstract: In common with many other viruses, adenoviruses code for a protease essential for the development of infectivity. Recombinant adenovirus protease was active in crude in vitro complementation assays but was inactive with peptide or purified protein substrates. Activity was reconstituted by a component of adenovirus virions, which was identified as GVQSLKRRRCF, a peptide derived from the virus protein pVI. Synthetic peptides were used to demonstrate that the cysteine is essential and that the disulphide-linked dimer is required for activity. It is proposed that the adenovirus protease is a cysteine protease and that its activation by the peptide involves thiol-disulphide interchange, which serves to expose the active site cysteine. This represents a novel strategy for controlling the activity of a protease that is required for virus maturation.
authors.affiliation: Division of Biochemistry and Molecular Biology, School of Biological and Medical Sciences, University of St. Andrews, Scotland.
authors.forename: A
authors.initials: A
authors.lastname: Webster
authors.forename: R T
authors.initials: RT
authors.lastname: Hay
authors.forename: G
authors.initials: G
authors.lastname: Kemp
mesh_terms: D000260:Adenoviruses, Human / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003067:Coenzymes / Q000302:isolation & purification / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral; D004220:Disulfides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D007700:Kinetics; D008969:Molecular Sequence Data; D009036:Moths; D010455:Peptides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D003067:Coenzymes; D004279:DNA, Viral; D004220:Disulfides; D010455:Peptides; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases; C091827:adenain