Tumor suppression in Xiphophorus by an accidentally acquired promoter.
Melanoma formation in the teleost Xiphophorus is caused by a dominant genetic locus, Tu. This locus includes the Xmrk oncogene, which encodes a receptor tyrosine kinase. Tumor induction is suppressed in wild-type fish by a tumor suppressor locus, R. Molecular genetic analyses revealed that the Tu locus emerged by nonhomologous recombination of the Xmrk proto-oncogene with a previously uncharacterized sequence, D. This event generated an additional copy of Xmrk with a new promoter. Suppression of the new Xmrk promoter by R in parental fish and its deregulation in hybrids explain the genetics of melanoma formation in Xiphophorus.
Adam
D
D
Genzentrum, Max-Planck-Institut für Biochemie, Martinsried, Germany.
Dimitrijevic
N
N
Schartl
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003532:Cyprinodontiformes / Q000235:genetics*; D004625:Embryo, Nonmammalian; D005393:Fish Diseases / Q000235:genetics*; D029941:Fish Proteins; D015723:Gene Library; D016147:Genes, Tumor Suppressor; D008545:Melanoma / Q000235:genetics / Q000662:veterinary*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009857:Oncogenes; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011519:Proto-Oncogenes; D020794:Receptor Protein-Tyrosine Kinases
D029941:Fish Proteins; D009838:Oligodeoxyribonucleotides; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases; C077101:Xmrk protein, Xiphophorus