Simultaneous determination of nicotinic acid and its two metabolites in human plasma using solid-phase extraction in combination with high performance liquid chromatography.
A high performance liquid chromatographic method for the simultaneous quantitative determination of nicotinic acid, nicotinamide and nicotinuric acid in human plasma is described. The method is based on solid phase extraction in combination with ion-paired reversed phase high performance liquid chromatography. Recoveries of nicotinic acid, nicotinamide and nicotinuric acid are 92.9, 95.8 and 87.8%, respectively. The procedure shown was applied to the determination of the plasma time-concentration profile of nicotinic acid, nicotinamide and nictotinuric acid after nicotinic acid ingestion in humans.
Miyauchi
Y
Y
Eisai Research Laboratories, Eisai Co., Ltd., Tokyo, Japan.
Sano
N
N
Nakamura
T
T
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D009525:Niacin / Q000097:blood* / Q000302:isolation & purification; D009536:Niacinamide / Q000097:blood* / Q000302:isolation & purification; D009539:Nicotinic Acids / Q000097:blood* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D009539:Nicotinic Acids; D009536:Niacinamide; D009525:Niacin; C002477:nicotinuric acid