Variants of vasoactive intestinal peptide in mouse mast cells and rat basophilic leukemia cells.
Radioimmunoassays for neuroendocrine vasoactive intestinal peptide (VIP1-28) detected 30-120 fmol of structurally related peptides in extracts of 10(7) mouse peritoneal mast cells, bone marrow-derived mast cells, cultured PT-18 and C1.MC/C57.1 lines of mast cells, and rat basophilic leukemia (RBL) cells. No VIP was found in peritoneal cells of mast cell-deficient WBB6F1-W/Wv mice, whereas the amounts extracted from peritoneal cells of the congenic normal (WBB6F1-+/+) mice were similar to those from cultured mouse mast cells. Sephadex G-25 gel filtration resolved two different-sized variants of VIP from mouse mast cells and RBL cells. Amino acid sequence analyses showed that the smaller variant is VIP10-28. The principal amino-terminally larger variant of VIP from C1.MC/C57.1 mouse mast cells and RBL cells exhibited amino acid sequence homology with VIP(-6)-28, and this sequence was established for the corresponding larger VIP from PT-18 mast cells. Polymerase chain reaction amplification of two different substituent sequences of prepro VIP in RBL cell RNA identified the VIP message. VIP10-28 was released from mouse mast cells concurrently with histamine by IgE-dependent stimulation. Rodent mast cell-derived VIP thus consists of both the truncated VIP10-28 and amino-terminally larger forms that appear to be generated by peptidolysis of a preproVIP similar to that found in neural cells.
Wershil
B K
BK
Department of Pathology, Beth Israel Hospital, Boston, Massachusetts.
Turck
C W
CW
Sreedharan
S P
SP
Yang
J
J
An
S
S
Galli
S J
SJ
Goetzl
E J
EJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D014407:Tumor Cells, Cultured; D014660:Vasoactive Intestinal Peptide / Q000031:analogs & derivatives / Q000378:metabolism*
D010446:Peptide Fragments; D011498:Protein Precursors; D014660:Vasoactive Intestinal Peptide; C047945:vasoactive intestinal peptide (10-28); C055594:preprovasoactive intestinal peptide