title: Characterization and expression of a novel human fatty acid-binding protein: the epidermal type (E-FABP).
abstract: Using PAGE--Autoradioblotting technique we have characterized an E--FABP in human epidermal cells that is distinct from liver-, heart-, intestine- and adipose tissue-FABPs. FABP radiobinding analysis was performed directly on protein extracts without prior partial purification. E-FABP has a Mr of approximately 15 kDa and binds oleic acid with high affinity but does not bind all-trans-, 13-cis- and 9-cis-retinoic acid nor all-trans-retinol. Expression levels of E-FABP were low in normal epidermis, higher in human cultured keratinocytes and still higher in psoriasis, a disease characterized by abnormal epidermal differentiation. These findings suggest that epidermal cells may have a distinct fatty acid metabolism compared to other tissues.
authors.affiliation: Clinique de Dermatologie, Hôpital Cantonal Universitaire, Genève, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Siegenthaler
authors.forename: R
authors.initials: R
authors.lastname: Hotz
authors.forename: D
authors.initials: D
authors.lastname: Chatellard-Gruaz
authors.forename: S
authors.initials: S
authors.lastname: Jaconi
authors.forename: J H
authors.initials: JH
authors.lastname: Saurat
mesh_terms: D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D002454:Cell Differentiation; D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000737:chemistry* / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D009363:Neoplasm Proteins; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D011565:Psoriasis / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism; D014316:Tritium; D025521:Tumor Suppressor Proteins; D014801:Vitamin A / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C493412:FABP5 protein, human; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D009829:Oleic Acids; D025521:Tumor Suppressor Proteins; D014316:Tritium; D014801:Vitamin A; D019301:Oleic Acid; D014212:Tretinoin