Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824.
The nucleotide sequence of three open reading frames in the acetone-production locus of Clostridium acetobutylicum ATCC824 has been established. The three gene products, corresponding to acetoacetate decarboxylase (EC 4.1.1.4) and both subunits of the acetoacetyl-CoA:acetate/butyrate:CoA transferase (EC 2.8.3.9) are transcribed in two convergently arranged operons. The intervening DNA region separating the two transcripts is characterized by an inverted repeat which appears capable of forming a stem-loop structure functioning as a Rho-independent transcription terminator in both directions.
Petersen
D J
DJ
F.A. Janssens Laboratorium voor Genetica, K.V. Leuven, Heverlee, Belgium.
Cary
J W
JW
Vanderleyden
J
J
Bennett
G N
GN
D000096:Acetone / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics* / Q000378:metabolism; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014158:Transcription, Genetic
D000214:Acyl Coenzyme A; D004269:DNA, Bacterial; D000096:Acetone; C010667:acetoacetyl CoA; D002262:Carboxy-Lyases; C023743:acetoacetate decarboxylase