Sequence-specific binding of transfer RNA by glyceraldehyde-3-phosphate dehydrogenase.
A transfer RNA (tRNA) binding protein present in HeLa cell nuclear extracts was purified and identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Studies with mutant tRNAs indicated that GAPDH recognizes both sequence and structural features in the RNA. GAPDH discriminated between wild-type tRNA and two tRNA mutants that are defective in nuclear export, which suggests that the protein may participate in RNA export. The cofactor nicotinamide adenine dinucleotide disrupted complex formation between tRNA and GAPDH and thus may share a common binding site with the RNA. Indirect immunofluorescence experiments showed that GAPDH is present in the nucleus as well as in the cytoplasm.
Singh
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Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605.
Green
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D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D004926:Escherichia coli / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012358:RNA, Transfer, Met / Q000737:chemistry / Q000378:metabolism*; D012362:RNA, Transfer, Ser / Q000378:metabolism; D012365:RNA, Transfer, Tyr / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic
D012358:RNA, Transfer, Met; D012362:RNA, Transfer, Ser; D012365:RNA, Transfer, Tyr; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases