title: PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants.
abstract: We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei.
authors.affiliation: Human Genome Center, Lawrence Livermore National Laboratory, University of California, Livermore.
authors.forename: P M
authors.initials: PM
authors.lastname: Kroisel
authors.forename: P A
authors.initials: PA
authors.lastname: Ioannou
authors.forename: P J
authors.initials: PJ
authors.lastname: de Jong
mesh_terms: D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004274:DNA, Recombinant / Q000032:analysis*; D005822:Genetic Vectors; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004274:DNA, Recombinant