Genomic DNA structure of a gene encoding cytosolic ascorbate peroxidase from Arabidopsis thaliana.
A genomic DNA clone encoding cytosolic ascorbate peroxidase was isolated from a genomic library of Arabidopsis thaliana, using a cDNA for the enzyme as a probe. Nucleotide sequence and primer extension analyses of this gene (APX1) revealed nine exons split by eight introns, one of which is inserted in the 5'-untranslated region. The exon/intron organization of the APX1 gene differs from that of the guaiacol peroxidase genes.
Kubo
A
A
Environmental Biology Division, National Institute for Environmental Studies, Ibaraki, Japan.
Saji
H
H
Tanaka
K
K
Kondo
N
N
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D015153:Blotting, Western; D003600:Cytosol / Q000201:enzymology*; D004247:DNA; D005091:Exons; D017343:Genes, Plant; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D004247:DNA; D010544:Peroxidases; D060387:Ascorbate Peroxidases