Detection of human chromosomes in somatic cell hybrids by PCR analysis.
The detection of human chromosomes in somatic cell hybrids is usually made by chromosomal analysis, Southern blot analysis with human probes, and starch-gel electrophoresis of isoenzymes. We describe here a new, quick, and very efficient method to detect human chromosomes in somatic cell hybrids between human and rodent (rat and mouse) cells. The method is based on the polymerase chain reaction to promote amplification of human DNA, using primers derived from localized genes or DNA fragments from each human chromosome.
Tripputi
P
P
Universitá degli Studi di Milano, Istituto di Scienze Biomediche San Gerardo, Monza, Italy.
Bacciocchi
G
G
Accolla
R S
RS
Mantero
G
G
De Bellis
G
G
Manoni
M
M
Cassani
G
G
Corneo
G
G
D000818:Animals; D002877:Chromosomes, Human; D004277:DNA, Single-Stranded; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats
D004277:DNA, Single-Stranded