[Studies of argyrophilic nuclear organizer region proteins in renal cell carcinoma. Its significance as a marker of proliferative activity].
Tumor grading protocols in renal cell carcinoma (RCC) have met with only limited success of prognostic value. The increase in argyrophilic nucleolar protein (AgNOR) count of a cell has been reported to reflect rDNA transcriptional activity and state of cellular activity. Therefore, the relationship between number of the AgNOR in nucleus and proliferative activity or clinical course in patients with RCC was evaluated. Using 6 cell lines derived from RCC patients and established in our institute, the number of AgNOR and doubling time of the cell were compared. These two values had statistically high negative correlation; a coefficient of correlation was -0.88. The AgNOR values in the cells of RCC (2.22 +/- 0.81) in 45 patients were significantly higher than those of normal proximal tubules (1.54 +/- 0.21) (p < 0.05). And, the numbers were increased with the progress of grades in RCC; 1.70 +/- 0.33 in Grade 1, 2.12 +/- 0.74 in Grade 2 and 3.19 +/- 0.19 in Grade 3. Moreover, the numbers of AgNOR were higher in infiltrating type such as sarcomatoid type or INF gamma. However, no significant relation in the AgNORs numbers was observed in subcell type of RCC. The patients with RCC having a counts of 2.5 or more of AgNOR showed a significantly poor prognosis. These results suggest that the determinations of the AgNOR numbers have a value to evaluation of the tumor proliferative activity in RCC patients.
Yamamoto
N
N
Department of Urology, Gifu University School of Medicine.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D002292:Carcinoma, Renal Cell / Q000175:diagnosis / Q000473:pathology*; D002455:Cell Division; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis; D016622:Silver Staining; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D009687:Nuclear Proteins