Interactions between tumoral MCF7 cells and fibroblasts on matrigel and purified laminin.
A reconstituted basement membrane (matrigel) and/or fibroblasts promote the growth of human breast tumors in athymic nude mice. We have investigated in vitro the effect of matrigel or purified glycoproteins (laminin and fibronectin) on tumoral MCF7 cells-fibroblasts interactions. In coculture on matrigel, MCF7 cells organized into clusters attached on top of fibroblasts aggregates. During the process resulting in tumor cells-fibroblasts aggregation, fibroblasts actively migrated while MCF7 cells were passively transported. Using purified proteins, specific antibodies and synthetic peptides, we show that cell aggregation induced by immobilized and soluble laminin is antagonized by exogenous fibronectin or fibronectin synthesized by fibroblasts.
Noël
A
A
Laboratories of Biology, CHU, Sart Tilman, University of Liege, Belgium.
Nusgens
B
B
Lapiere
C H
CH
Foidart
J M
JM
D000595:Amino Acid Sequence; D001672:Biocompatible Materials; D001940:Breast / Q000166:cytology / Q000502:physiology; D001943:Breast Neoplasms / Q000473:pathology* / Q000503:physiopathology; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D004338:Drug Combinations; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D011509:Proteoglycans / Q000494:pharmacology*; D012867:Skin / Q000166:cytology; D012879:Skin Physiological Phenomena; D014407:Tumor Cells, Cultured
D001672:Biocompatible Materials; D004338:Drug Combinations; D007797:Laminin; D009842:Oligopeptides; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; C046617:arginyl-glycyl-aspartyl-serine