title: Analysis of aphidicolin-induced chromosome fragility in the domestic pig (Sus scrofa).
abstract: Aphidicolin (APC)-sensitive fragile sites were identified in chromosome preparations from peripheral lymphocyte cultures of 12 four-way crossbred pigs. A chi 2 analysis demonstrated that aphidicolin-induced breakage events and previously reported in vivo chromosome rearrangement events were not independent. Comparison of expected Poisson and negative binomial distributions to observed breakage patterns indicated that the negative binomial distribution provided a better fit to experimental data. The negative binomial distribution is consistent with a distribution of breakage rates, i.e., non-constant rates of breakage at chromosomal loci across the genome.
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, IN 47007.
authors.forename: P K
authors.initials: PK
authors.lastname: Riggs
authors.forename: T
authors.initials: T
authors.lastname: Kuczek
authors.forename: C L
authors.initials: CL
authors.lastname: Chrisman
authors.forename: C A
authors.initials: CA
authors.lastname: Bidwell
mesh_terms: D000818:Animals; D016590:Aphidicolin / Q000633:toxicity*; D016010:Binomial Distribution; D016009:Chi-Square Distribution; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D004305:Dose-Response Relationship, Drug; D005260:Female; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D016012:Poisson Distribution; D013552:Swine / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D009153:Mutagens; D016590:Aphidicolin