[Comparative study on antigens of Cysticercus cellulosae by ELIB and ELISA].
Urea-soluble antigens (Ag-u) and water-soluble antigens (Ag-w) of Cysticercus cellulosae were studied by using ELIB and ELISA, respectively. The results showed that using Ag-u and Ag-w, 9 and 14 bands of polypeptides could be recognized by sera from cysticercosis patients; 4 and 10 bands could be recognized by sera from hydatidosis patients; whereas 4 and 8 bands could be recognized by sera from clornochiasis patients. Using ELISA at serum dilutions of 1:400, the positive rate of sera against Ag-u and Ag-w was 96.6% and 86.6%, respectively in 60 cysticercosis patients; 16.6% and 46.6% respectively in 30 hydatidosis patients and negative in 24 clornorchiasis patients and 50 normal healthy persons, indicating that both Ag-u and Ag-w share common antigen components causing cross reaction with the sera from hydatidosis and clonorchiasis patients. However, such components were fewer in Ag-u than in Ag-w. In view of the fact that Ag-u is superior to Ag-w in both sensitivity and specificity, Ag-u might be better used for the diagnosis of cysticercosis.
Wang
K H
KH
Department of Parasitology, Harbin Medical University.
Xu
Z J
ZJ
Zeng
D K
DK
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood / Q000737:chemistry* / Q000145:classification; D015153:Blotting, Western; D003003:Clonorchiasis / Q000097:blood; D003551:Cysticercosis / Q000097:blood; D003552:Cysticercus / Q000276:immunology*; D004443:Echinococcosis / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans
D000947:Antigens, Helminth