Chemiluminescence immunoassay to detect anti-HTLV-I antibodies.
A fully automated chemiluminescence immunoassay was developed for the detection of antibodies to HTLV-I. We used partially purified viral antigens coated on small polystyrene beads together with acridinium ester-labeled anti-human immunoglobulin G mouse immunoglobulin G in this method. A good agreement was observed between our proposed method, the indirect immunofluorescence assay, the particle-agglutination test and the enzyme immunoassay. This new method, which is simple, sensitive, specific and rapid, should be useful for mass screening of anti-HTLV-I antibodies.
Kamihira
S
S
Blood Transfusion Service, School of Medicine, Nagasaki University.
Nakashima
S
S
Saitoh
S
S
Kawamoto
M
M
Kawashima
Y
Y
Shimamoto
M
M
D015481:HTLV-I Antibodies / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements
D015481:HTLV-I Antibodies