The effect of annexin IV and VI on the fluidity of phosphatidylserine/phosphatidylcholine bilayers studied with the use of 5-deoxylstearate spin label.
An effect of annexin IV and VI on the fluidity of phosphatidylserine/phosphatidylcholine (PS/PC) membranes was studied by spin labeling technique with the use of 5-doxylstearic acid. It was found that calcium ions at micromolar concentrations induced a marked decrease in the order parameter of PS/PC membranes. This effect was completely abolished by both annexins. The influence of annexins on the mobility of fatty acid chains in the studied region in PE/PC membranes was insignificant.
Sobota
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A
M. Nencki Institute of Experimental Biology, Department of Cell Biology, Warsaw, Poland.
Bandorowicz
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J
Jezierski
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Sikorski
A F
AF
D017314:Annexin A4 / Q000737:chemistry*; D017317:Annexin A6 / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D066298:In Vitro Techniques; D008051:Lipid Bilayers; D008560:Membrane Fluidity; D008567:Membranes, Artificial; D010713:Phosphatidylcholines / Q000737:chemistry*; D010718:Phosphatidylserines / Q000737:chemistry*; D013113:Spin Labels; D013696:Temperature
D017314:Annexin A4; D017317:Annexin A6; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D013113:Spin Labels; D002118:Calcium