Cloning of a functional cDNA for human cytidine deaminase (CDD) and its use as a marker of monocyte/macrophage differentiation.
We have identified a cDNA clone for human cytidine deaminase (EC 3.5.4.5) during an investigation which aimed at cloning novel gene expression products related to monocyte/macrophage differentiation. The derived amino acid sequence of the clone comprises 145 residues yielding a molecular mass for the polypeptide of 16.1 kDa and exhibits a nearly 50% homology to cytidine deaminase from Bacillus subtilis. Cytidine deaminase activity of the cloned sequence could be demonstrated in a prokaryotic expression system. The mRNA is highly expressed in granulocytes while expression is very low in fibroblasts, chondrocytes, monocytes, and T- as well as B-cell lines. The mRNA can be induced in monocytes, the monocytoid cell line U937 and the myeloblastic line HL 60 by the differentiation inducer calcitriol.
Kühn
K
K
Max-Planck Society, Institute for Immunology, University of Erlangen-Nürnberg, Germany.
Bertling
W M
WM
Emmrich
F
F
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology; D001483:Base Sequence; D015415:Biomarkers; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003564:Cytidine Deaminase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015723:Gene Library; D006098:Granulocytes / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000201:enzymology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology* / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D015415:Biomarkers; D012333:RNA, Messenger; D004247:DNA; D003564:Cytidine Deaminase; D002117:Calcitriol