Intracerebral and subcutaneous xenografts of human SCLC in the nude rat: comparison of monoclonal antibody localization and tumor infiltrating lymphocytes.
In the WAG/Rij nude rat, subcutaneous (s.c.) and intracerebral (i.c.) xenografts of the human SCLC cell line GLC-28 were evaluated for their growth behavior, in vivo monoclonal antibody binding and presence of tumor infiltrating lymphocytes. For the i.c. xenografts, two models of cerebral tumor growth were studied, one in the cerebral cortex and one in the lateral ventricle of the brain. In the s.c. and both i.c. xenografts models, in vivo localization of anti-carcinoma moab MOC-31 occurred within 4 hours after i.p. injection, with a maximal binding at 24 h after injection. A pronounced tumor infiltration of predominantly NK cells was observed for s.c. and intraventricular xenografts, but not for the GLC-28 tumors xenografted in the cerebral cortex. The presented nude rat/GLC-28 xenograft models may be used for the in vivo testing of experimental imaging techniques or alternative treatment strategies relevant to brain metastases of human SCLC.
Bulte
J W
JW
Department of Clinical Immunology, University of Groningen, The Netherlands.
Go
K G
KG
Zuiderveen
F
F
The
T H
TH
de Leij
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000473:pathology*; D018288:Carcinoma, Small Cell / Q000473:pathology*; D006801:Humans; D007279:Injections, Subcutaneous; D007694:Killer Cells, Natural; D008175:Lung Neoplasms / Q000473:pathology*; D016246:Lymphocytes, Tumor-Infiltrating; D009368:Neoplasm Transplantation; D051381:Rats; D011923:Rats, Nude; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal