title: Presence of two independent chromosomes in the Brucella melitensis 16M genome.
abstract: Mapping the restriction fragments of the Brucella melitensis 16M genome with a new restriction endonuclease, PacI, which cut the DNA into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. Pulsed-field gel electrophoresis of intact DNA revealed two bands migrating the expected distances. These replicons were identified as two unique and independent chromosomes by the presence of rRNA operons and genes for heat shock proteins mapping to separate replicons.
authors.affiliation: Unité 65, Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine, Nmes, France.
authors.forename: S
authors.initials: S
authors.lastname: Michaux
authors.forename: J
authors.initials: J
authors.lastname: Paillisson
authors.forename: M J
authors.initials: MJ
authors.lastname: Carles-Nurit
authors.forename: G
authors.initials: G
authors.lastname: Bourg
authors.forename: A
authors.initials: A
authors.lastname: Allardet-Servent
authors.forename: M
authors.initials: M
authors.lastname: Ramuz
mesh_terms: D017347:Brucella melitensis / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004269:DNA, Bacterial / Q000235:genetics / Q000648:ultrastructure*; D016521:Electrophoresis, Gel, Pulsed-Field; D012093:Replicon; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial