Removal of lens epithelial cells following loosening of the junctional complex.
We previously reported a new method to remove residual lens epithelial cells--dispersion aspiration. The cells were loosened from their junctional complexes with Dispase, a proteolytic enzyme. To avoid intraocular tissue damage, the enzyme preparation was dissolved in sodium hyaluronate and injected into the capsular bag, which was carefully preserved during endocapsular cataract surgery. The cells were then removed by minimum irrigation/aspiration. In this study we incorporated ethylenediaminetetraacetic acid (EDTA), a calcium chelating agent for separating epithelial cells in tissue culture, into the procedure. The results of experiments in vitro and in rabbits suggest that this procedure also removed cells effectively with negligible damage to the zonules and corneal endothelium.
Nishi
O
O
Nishi Eye Hospital, Osaka, Japan.
Nishi
K
K
Hikida
M
M
D000818:Animals; D002387:Cataract Extraction / Q000379:methods*; D004492:Edetic Acid / Q000008:administration & dosage; D010450:Endopeptidases / Q000008:administration & dosage; D004728:Endothelium, Corneal / Q000187:drug effects / Q000648:ultrastructure; D004848:Epithelium / Q000187:drug effects / Q000601:surgery; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage; D007903:Lens Capsule, Crystalline / Q000601:surgery; D007908:Lens, Crystalline / Q000187:drug effects / Q000601:surgery*; D007910:Lenses, Intraocular; D011817:Rabbits; D013396:Suction
D006820:Hyaluronic Acid; D004492:Edetic Acid; D010450:Endopeptidases; C023139:dispase