The FtsZ protein of Bacillus subtilis is localized at the division site and has GTPase activity that is dependent upon FtsZ concentration.
The ftsZ gene is essential for cell division in both Escherichia coli and Bacillus subtilis. In E. coli FtsZ forms a cytokinetic ring at the division site whose formation is under cell-cycle control. In addition, the FtsZ from E. coli has a GTPase activity that shows an unusual lag in vitro. In this study we show that FtsZ in Bacillus subtilis forms a ring that is at the tip of the invaginating septum. The FtsZ ring is dynamic since it is formed as division is initiated, changes diameter during septation, and disperses upon completion of septation. In vitro the purified FtsZ from B. subtilis exhibits a GTPase activity without a demonstrable lag, but the GTPase activity is markedly dependent upon the FtsZ concentration, suggesting that the FtsZ protein must oligomerize to express the GTPase activity.
Wang
X
X
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.
Lutkenhaus
J
J
D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000528:radiation effects; D002455:Cell Division; D003432:Cross-Linking Reagents; D003598:Cytoskeletal Proteins; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development; D020558:GTP Phosphohydrolases / Q000302:isolation & purification* / Q000378:metabolism / Q000528:radiation effects; D006160:Guanosine Triphosphate / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D011487:Protein Conformation; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases