title: Differential expression of GABA transporter-1 messenger RNA in subpopulations of GABA neurones.
abstract: Using in situ hybridisation with an oligonucleotide probe, the regional distribution of GABA transporter-1 messenger RNA was determined in the adult rat CNS. Overall, the distribution of GABA transporter-1 mRNA was similar to that of glutamate decarboxylase-67 mRNA, consistent with an expression in GABA neurones. However, in cerebellar cortex, Purkinje cells which express high levels of glutamate decarboxylase-67 mRNA did not express GABA transporter-1 mRNA, whereas Bergmann glia expressed GABA transporter-1 mRNA at high levels. In some other brain regions, including the inferior colliculus and reticular thalamus, there was no GABA transporter-1, although there were neurones which expressed glutamate decarboxylase-67 messenger RNA at high levels.
authors.affiliation: Division of Biochemistry, UMDS, University of London, Guy's Hospital, UK.
authors.forename: M
authors.initials: M
authors.lastname: Rattray
authors.forename: J V
authors.initials: JV
authors.lastname: Priestley
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis*; D002525:Cerebellar Cortex / Q000378:metabolism*; D050485:GABA Plasma Membrane Transport Proteins; D015870:Gene Expression; D005968:Glutamate Decarboxylase / Q000096:biosynthesis; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D015345:Oligonucleotide Probes; D027361:Organic Anion Transporters; D011689:Purkinje Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D015345:Oligonucleotide Probes; D027361:Organic Anion Transporters; D012333:RNA, Messenger; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase