title: The GTPase dynamin binds to and is activated by a subset of SH3 domains.
abstract: Src homology 3 (SH3) domains have been implicated in mediating protein-protein interactions in receptor signaling processes; however, the precise role of this domain remains unclear. In this report, affinity purification techniques were used to identify the GTPase dynamin as an SH3 domain-binding protein. Selective binding to a subset of 15 different recombinant SH3 domains occurs through proline-rich sequence motifs similar to those that mediate the interaction of the SH3 domains of Grb2 and Abl proteins to the guanine nucleotide exchange protein, Sos, and to the 3BP1 protein, respectively. Dynamin GTPase activity is stimulated by several of the bound SH3 domains, suggesting that the function of the SH3 module is not restricted to protein-protein interactions but may also include the interactive regulation of GTP-binding proteins.
authors.affiliation: Ludwig Institute for Cancer Research, London, England.
authors.forename: I
authors.initials: I
authors.lastname: Gout
authors.forename: R
authors.initials: R
authors.lastname: Dhand
authors.forename: I D
authors.initials: ID
authors.lastname: Hiles
authors.forename: M J
authors.initials: MJ
authors.lastname: Fry
authors.forename: G
authors.initials: G
authors.lastname: Panayotou
authors.forename: P
authors.initials: P
authors.lastname: Das
authors.forename: O
authors.initials: O
authors.lastname: Truong
authors.forename: N F
authors.initials: NF
authors.lastname: Totty
authors.forename: J
authors.initials: J
authors.lastname: Hsuan
authors.forename: G W
authors.initials: GW
authors.lastname: Booker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D004330:Drosophila / Q000235:genetics; D034281:Dynamins; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000302:isolation & purification / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D005982:Glutathione Transferase; D020558:GTP Phosphohydrolases; D034281:Dynamins