Fragmentation of DNA in P388D1 macrophages exposed to oxidised low-density lipoprotein.
Exposing a macrophage-like murine cell line to copper-oxidised low-density lipoprotein led to DNA fragmentation which was inhibited by the putative Ca2+/Mg2+ endonuclease inhibitor, zinc sulphate. DNA fragmentation preceded loss of membrane impermeability. These results suggest that apoptosis may be a mechanism of macrophage foam cell death in atherosclerotic lesions in the arterial wall.
Reid
V C
VC
Department of Pathology, University of Cambridge, UK.
Hardwick
S J
SJ
Mitchinson
M J
MJ
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*
D000227:Adenine Nucleotides; D008077:Lipoproteins, LDL; D004247:DNA