title: The ability of apocytochrome C to pass lipid bilayer is relevant with its folding state.
abstract: The translocation ability of two different species of apocytochrome c(horse heart, Candida krusei) across the soybean phospholipid vesicles decreased in the order: C. krusei > horse heart. Theoretical calculations of the amphiphilicity of their N- and C-terminal alpha-helix formation and the release experiment using calcein-enclosed soybean phospholipid vesicles showed that there was no direct relevance between their import ratio and the amphiphilic helicity. On the other hand, taking the advantage of circular dichroism technique, a more loosely folded structure of C. krusei following the interaction with soybean phospholipid vesicles was observed which should be responsible for the difference in translocation rate.
authors.affiliation: National laboratory of Biomacromolecules, Academia Sinica, Beijing, People's Republic of China.
authors.forename: X S
authors.initials: XS
authors.lastname: Wang
authors.forename: F Y
authors.initials: FY
authors.lastname: Yang
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism*; D002175:Candida / Q000737:chemistry; D002942:Circular Dichroism; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism*; D045304:Cytochromes c; D005452:Fluoresceins / Q000737:chemistry; D006736:Horses; D008051:Lipid Bilayers / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry; D010743:Phospholipids / Q000378:metabolism; D017510:Protein Folding
chemical_list: D001059:Apoproteins; D003574:Cytochrome c Group; D005452:Fluoresceins; D008051:Lipid Bilayers; D010743:Phospholipids; D045304:Cytochromes c; C007740:fluorexon