Involvement of thiols in the induction of inward current induced by silver in frog skeletal muscle membrane.
Exposure of voltage-clamped frog skeletal muscle fibres to silver caused a maintained inward current which could be carried by Ca2+, Mg2+ or Na+. Inorganic Ca2+ channel blockers and dithiothreitol (SH reducing agent) diminished this current, but a Na+ channel blocker did not. Thus, silver activates the Ca2+ channel by acting on SH groups in a Ca2+ channel protein.
Aoki
T
T
Department of Food and Nutrition Science, Gifu City Women's College, Japan.
Nihonyanagi
K
K
Oba
T
T
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D004229:Dithiothreitol / Q000494:pharmacology; D004553:Electric Conductivity; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D011892:Rana catesbeiana; D011898:Ranidae; D012834:Silver / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000502:physiology*
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D013438:Sulfhydryl Compounds; D012834:Silver; D004229:Dithiothreitol