In vitro cytotoxic and cell transforming activities exerted by the pesticides cyanazine, dithianon, diflubenzuron, procymidone, and vinclozolin on BALB/c 3T3 cells.
Cytotoxic and cell transforming activities of the pesticides cyanazine, diflubenzuron, dithianon, procymidone, and vinclozolin were investigated in vitro by utilizing the BALB/c 3T3 cell transformation test performed in the presence or in the absence of S-9 mix as an exogenous bioactivation system for the chemicals. All the assayed pesticides were cytotoxic in the absence of S-9 mix, whereas only dithianon exerted cytotoxic effects in the presence of metabolic activation. All the chemicals tested did induce BALB/c 3T3 cell transformation, to a various extent, in the absence of S-9 mix. Cell transforming ability of cyanazine and diflubenzuron was not detectable in the presence of S-9.
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Istituto di Cancerologia, Università di Bologna, Italy.
Colacci
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Grilli
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D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D000880:Anthraquinones / Q000633:toxicity; D001643:Bridged Bicyclo Compounds / Q000633:toxicity; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D004132:Diflubenzuron / Q000633:toxicity*; D005659:Fungicides, Industrial / Q000633:toxicity*; D008110:Liver Extracts / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000201:enzymology; D010080:Oxazoles / Q000633:toxicity; D014170:Transformation, Genetic; D014227:Triazines / Q000633:toxicity*
D000880:Anthraquinones; D001643:Bridged Bicyclo Compounds; D002273:Carcinogens; D005659:Fungicides, Industrial; D008110:Liver Extracts; D010080:Oxazoles; D014227:Triazines; C018529:dithianone; C035988:procymidone; D004132:Diflubenzuron; C025643:vinclozolin; C007168:cyanazine