Membrane topology of the 22 kDa integral peroxisomal membrane protein.
In order to study the membrane topology and the possible function of the rat liver 22 kDa integral peroxisomal membrane protein (PMP 22) at a molecular level, we have cloned PMP 22 from a lambda gt11 expression library and sequenced its cDNA. Hydropathy analysis of the deduced primary structure indicates 4 putative transmembrane segments. The accessibility to exogenous aminopeptidase of PMP 22 in intact peroxisomes suggests that the N-terminus faces the cytosol. A model of the topology of PMP 22 in the peroxisomal membrane is discussed. Homology studies revealed a striking similarity with the Mpv 17 gene product. Lack of this membrane protein causes nephrotic syndrome in mice.
Kaldi
K
K
Institut für Biochemie I, Universität Heidelberg, Germany.
Diestelkötter
P
P
Stenbeck
G
G
Auerbach
S
S
Jäkle
U
U
Mägert
H J
HJ
Wieland
F T
FT
Just
W W
WW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D007425:Intracellular Membranes / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008830:Microbodies / Q000737:chemistry*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D008565:Membrane Proteins; C065292:Mpv17 protein, mouse; D011506:Proteins; C489786:Pxmp2 protein, mouse; C079231:Pxmp2 protein, rat; D004247:DNA