[Correlations between specific binding of labeled adrenoligands of the brain and plasma testosterone in mice].
Correlations between specific binding of labeled adrenoligands in the frontal cortex and hypothalamus and peripheral blood plasma testosterone were studied in BALB/cLac, CBA/Lac, and S57BL/6J male mice. A negative correlation was detected between blood testosterone and the extent of specific binding of 3H-clonidine, a 1.6 nM alpha 2-adrenoceptor agonist, in the hypothalamus. No correlations were revealed between specific binding of 3H-clonidine in the frontal cortex and binding of the rest radioligands (0.4 nM alpha 1-adrenoceptor antagonist 3H-prazosin and 1.6 nM beta-adrenoceptor antagonist 3H-dihydroalprenolol) in both brain segments and blood testosterone level. Further experiments in six mouse strains (A/He, BALB/cLac, CBA/Lac, C57BL/6J, DD, and YT) revealed a reliable negative genotypic correlation between hypothalamic maximal specific binding of 3H-clonidine and testosterone level. The detected feedback may be an important regulatory mechanism of the hypothalamo-hypophyseo-gonadal system in male mice.
Shishkina
G T
GT
Naumenko
E V
EV
D000818:Animals; D003000:Clonidine / Q000378:metabolism; D005246:Feedback; D005625:Frontal Lobe / Q000378:metabolism*; D005838:Genotype; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D011869:Radioligand Assay; D011941:Receptors, Adrenergic / Q000378:metabolism*; D013045:Species Specificity; D013739:Testosterone / Q000097:blood*
D011941:Receptors, Adrenergic; D013739:Testosterone; D003000:Clonidine