Assignments of 37 YAC clones to R-banded chromosomes by fluorescent in situ hybridization.
Fluorescent in situ hybridization was used to assign 37 yeast artificial chromosomes (YACs) from the CEPH (Centre d'Etude du Polymorphisme Humain) libraries to R-banded metaphases stained with propidium iodide. The use of the whole yeast DNA as probe, allows rapid mapping and characterization of YAC clones. Of 37 YAC clones, 11 were found cytogenetically chimeric (30%). The YAC clones reported in this study can be used to isolate and characterize targeted regions involved in genetic diseases and cancer breakpoints.
Slim
R
R
Laboratoire de Cytogénétique et de Génétique Oncologiques (CNRS URA 1158), Institut Gustave Roussy, Villejuif, France.
Machavoine
C
C
Bernheim
A
A
D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes; D008297:Male