title: Intra-amniotic inoculation of pigtailed macaque (Macaca nemestrina) fetuses with SIV and HIV-1.
abstract: Six pregnant pigtailed macaques (Macaca nemestrina) were inoculated intra-amniotically (i.a.) with SIVMne. All became viremic and seroconverted; three viable offspring were SIV-positive and at autopsy showed disseminated viral infection; one of three abortuses had SIV-infected thymic macrophages. Three of five pregnant macaques inoculated i.v. and/or i.a. with HIV-1LAI became virus-positive, and four seroconverted, suggesting fetal-maternal transmission. One abortus had HIV-1-antigen in lymph nodes and brain; one infant, culture-positive at birth, died at age 11 days of disseminated HIV-1 infection.
authors.affiliation: Department of Pediatrics, University of Washington School of Medicine, Seattle 98195.
authors.forename: H D
authors.initials: HD
authors.lastname: Ochs
authors.forename: W R
authors.initials: WR
authors.lastname: Morton
authors.forename: L D
authors.initials: LD
authors.lastname: Kuller
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.forename: C C
authors.initials: CC
authors.lastname: Tsai
authors.forename: M B
authors.initials: MB
authors.lastname: Agy
authors.forename: R E
authors.initials: RE
authors.lastname: Benveniste
mesh_terms: D000650:Amnion / Q000382:microbiology; D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000150:complications / Q000382:microbiology / Q000635:transmission*; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification; D008254:Macaca nemestrina; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology / Q000635:transmission*; D015302:Simian Immunodeficiency Virus / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D015483:HIV Antibodies