Lane distortions in gel electrophoresis patterns.
Distortions effects were studied as a function of gel environment, apparatus design and buffer type. Outward lanes distortions pronounced in low conductivity buffers for both continuous buffer systems and stacking gels, were field strength dependent. In discontinuous buffer systems, the moving boundary of the Laemmli buffer system deformed depending on the environment. In the Michov buffer system, the high conductivity resolving gel surrounded by a low conductivity electrode buffer, displayed straight pathways until a field strength of 15 V/cm, permitting to obtain information about lanes distortions depending on the acrylamide matrix structure. A decreased ammonium persulfate concentration, e.g. to 0.03%, induced phase segregation in gels at low temperature during the run: a critical endpoint near 11 degrees C for a gel in the Michov buffer system under conditions of electrophoresis was supposed. Gel collapse resulted in inward deviations in the lanes of the pattern and also faint bands in the zones where the gel was retracted. Besides this effect of catalyst concentration, the inwardly distorted pattern depended on temperature, field strength, solvent and hydrolysis due to gel aging. Amendments for overcoming lane distortions are suggested.
Starita-Geribaldi
M
M
Laboratoire de Biochimie, UFR de Médecine, Nice, France.
Houri
A
A
Sudaka
P
P
D000645:Ammonium Sulfate; D002021:Buffers; D004560:Electricity; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005998:Glycine; D006868:Hydrolysis; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification; D011786:Quality Control; D012997:Solvents; D013696:Temperature; D014325:Tromethamine
D002021:Buffers; D011506:Proteins; D012997:Solvents; D014325:Tromethamine; C031276:ammonium peroxydisulfate; D000645:Ammonium Sulfate; D005998:Glycine