Ornithine decarboxylase inactivation by putrescine: involvement of lipid peroxidation.
Effect of polyamines on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced reduction of lipid peroxidation was studied. Putrescine protected this lowering of lipid peroxidation in a concentration-dependent manner, but spermidine or spermine could not do so. Putrescine also inhibited the TPA-induced ornithine decarboxylase (ODC) activity and lowered the free sulfhydryl content of TPA exposed mouse skin. These observations indicate that putrescine inactivates ODC probably by lowering SH groups through lipid peroxidation.
Gupta
K P
KP
Laboratory of Environmental Carcinogenesis, Industrial Toxicology Research Center, M. G. Marg, Lucknow, India.
Mehrotra
N K
NK
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects*; D051379:Mice; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D065108:Ornithine Decarboxylase Inhibitors; D011700:Putrescine / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D065108:Ornithine Decarboxylase Inhibitors; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D017392:Thiobarbituric Acid Reactive Substances; D013096:Spermine; D013755:Tetradecanoylphorbol Acetate; D013095:Spermidine; D011700:Putrescine