Divergent effects of butyrate on the alkaline phosphatases of SW-620 cells.
The human colon cancer cell line SW-620 produces two alkaline phosphatases (ALPs) which are not expressed by normal colon. They are the heat-stable, term-placental and the heat-labile, L-homoarginine-sensitive, liver/bone/kidney ALPs. Butyrate, an ALP inducer, has strikingly dissimilar effects on the activity of these enzymes: whereas high (2.0 mM) butyrate concentrations exclusively induce increased activity levels of liver/bone/kidney ALP, low (0.5 mM) concentrations increase the activity of both, albeit induction of term-placental ALP is less pronounced. These observations indicate that the effect of butyrate on the two ALPs is non-coordinate and suggest that their expression by SW-620 cells is independently modulated.
Herz
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Department of Pathology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 10467.
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000187:drug effects*; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D003110:Colonic Neoplasms; D004790:Enzyme Induction / Q000187:drug effects; D004795:Enzyme Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013816:Thermodynamics; D014407:Tumor Cells, Cultured
D002087:Butyrates; D020148:Butyric Acid; D000469:Alkaline Phosphatase