title: Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons.
abstract: The polymerase chain reaction (PCR) was used to amplify approximately 1.5 kb segments of DNA containing complete Shiga-like toxin type II operons from Escherichia coli serotypes OX3:H21 and O111:H-. These fragments were cloned and DNA sequence analysis identified further variations compared with published SLT-II sequences.
authors.affiliation: Department of Microbiology, Adelaide Children's Hospital, South Australia.
authors.forename: A W
authors.initials: AW
authors.lastname: Paton
authors.forename: J C
authors.initials: JC
authors.lastname: Paton
authors.forename: P A
authors.initials: PA
authors.lastname: Manning
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D014644:Genetic Variation; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D022641:Shiga Toxin 2
chemical_list: D001427:Bacterial Toxins; D017931:DNA Primers; D022641:Shiga Toxin 2