Validation of nitrogen-13-ammonia tracer kinetic model for quantification of myocardial blood flow using PET.
Positron emission tomography has been shown to provide quantitative estimates of myocardial blood flow using 13N-ammonia and 15O-water. In a validation study, myocardial blood flow was noninvasively determined in 11 open-chest anesthetized dogs using dynamic positron emission tomography. The radiopharmaceuticals 13N-ammonia and 15O-water were intravenously administered and measurements were carried out at rest and following pharmacological vasodilation to assess blood flow over a range from 53 to 580 ml/100 g/min. Quantification of blood flow based on tracer kinetic modeling of 13N-ammonia data correlated closely with myocardial blood flow determined by microspheres (y = 0.944 x +7.22, r = 0.986) and with the 15O-water injection technique y = 1.054 x -15.8 (r = 0.99). The use of 13N-ammonia with positron emission tomography enables the accurate quantification of myocardial blood flow. Using this technique, uncomplicated study protocols simplify the measurement procedures while providing excellent qualitative and quantitative information.
Muzik
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Department of Internal Medicine, University of Michigan Hospitals, Ann Arbor 48109-0028.
Beanlands
R S
RS
Hutchins
G D
GD
Mangner
T J
TJ
Nguyen
N
N
Schwaiger
M
M
D000641:Ammonia; D000818:Animals; D003326:Coronary Circulation; D004285:Dogs; D009590:Nitrogen Radioisotopes / Q000493:pharmacokinetics; D010104:Oxygen Radioisotopes; D014055:Tomography, Emission-Computed
D009590:Nitrogen Radioisotopes; D010104:Oxygen Radioisotopes; D000641:Ammonia