title: Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo.
abstract: The kinetics of processing of glyceride-modified prolipoprotein that accumulated in globomycin-treated Escherichia coli has been found to be affected by sec mutations, i.e., secA, secE, secY, secD, and secF, and by metabolic poisons which affect proton motive force (PMF). The effect of sec mutations on processing of glyceride-modified prolipoprotein in vivo was not due to a secondary effect on PMF. Neither a secF mutation nor metabolic poisons affected the processing of previously accumulated proOmpA protein in vivo, suggesting that the requirements for functional sec gene products and PMF are specific to the processing of lipoprotein precursors by signal peptidase II.
authors.affiliation: Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
authors.forename: N
authors.initials: N
authors.lastname: Kosic
authors.forename: M
authors.initials: M
authors.lastname: Sugai
authors.forename: C K
authors.initials: CK
authors.lastname: Fan
authors.forename: H C
authors.initials: HC
authors.lastname: Wu
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D001149:Arsenates / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005838:Genotype; D007700:Kinetics; D008074:Lipoproteins / Q000096:biosynthesis*; D008715:Methionine / Q000378:metabolism; D016296:Mutagenesis; D010455:Peptides; D011392:Proline / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D019810:Sodium Azide; D013462:Sulfur Radioisotopes; D014316:Tritium
chemical_list: D000900:Anti-Bacterial Agents; D001149:Arsenates; D001386:Azides; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C099932:Lpp protein, E coli; D010455:Peptides; D011498:Protein Precursors; D013462:Sulfur Radioisotopes; D014316:Tritium; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; C017339:globomycin; D019810:Sodium Azide; D011392:Proline; D008715:Methionine; C025657:arsenic acid