title: Simultaneous determination of propranolol or metoprolol in the presence of butyrophenones in human plasma by gas chromatography with mass spectrometry.
abstract: A method has been developed for the simultaneous gas chromatographic determination of propranolol or metoprolol and butyrophenones butyrophenones in human plasma in vitro. The drugs were extracted from plasma by solid-phase extraction. Calibration graphs were linear in the 50-300-ng/mL range. The recovery of the compounds was 80-90%. The sensitivity of the method was adequate for the determination of the drugs at toxic concentrations and at therapeutic levels. It is a simple, rapid, and reproducible method for the simultaneous determination of propranolol or metoprolol and some butyrophenones. The minimum detectable concentration was 50 ng/mL with a flame ionization detector and 10 ng/mL with a mass spectrometer detector.
authors.affiliation: Dipartimento di Scienze Farmaceutiche-Università di Ferrara, Italy.
authors.forename: M P
authors.initials: MP
authors.lastname: Quaglio
authors.forename: A M
authors.initials: AM
authors.lastname: Bellini
authors.forename: L
authors.initials: L
authors.lastname: Minozzi
authors.forename: G
authors.initials: G
authors.lastname: Frisina
authors.forename: F
authors.initials: F
authors.lastname: Testoni
mesh_terms: D002090:Butyrophenones / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D008790:Metoprolol / Q000097:blood*; D011433:Propranolol / Q000097:blood*
chemical_list: D002090:Butyrophenones; D007202:Indicators and Reagents; D011433:Propranolol; D008790:Metoprolol