title: Robust and inexpensive equipment design for polymerase chain reaction detection of sequence mutations. Cystic fibrosis in a mother and 2 children analysed.
abstract: Every polymerase chain reaction (PCR) requires use of a temperature cycler for about 3 hours. Since there are many diagnostic tests using this technology, it is important that robust but inexpensive machinery is available. Such a stand-alone machine has been designed and used to analyse an interesting family in which a mother and her 2 children were diagnosed as having cystic fibrosis.
authors.affiliation: University of Cape Town.
authors.forename: L R
authors.initials: LR
authors.lastname: Purves
authors.forename: R
authors.initials: R
authors.lastname: Rubinstein
authors.forename: J
authors.initials: J
authors.lastname: Maxwell
authors.forename: F
authors.initials: F
authors.lastname: Leisegang
authors.forename: J
authors.initials: J
authors.lastname: Ireland
mesh_terms: D000328:Adult; D003550:Cystic Fibrosis / Q000175:diagnosis* / Q000235:genetics; D004867:Equipment Design; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009154:Mutation; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000295:instrumentation*