In vivo 31P NMR measurement of glucose-6-phosphate in the rat muscle after exercise.
Comparison of 31P NMR spectra of the rat gastrocnemius, obtained in vivo and from PCA extracts, after electrically induced contractions, demonstrates that glucose-6-phosphate (G6P) is the major metabolite in the low-field part of the PME spectral region. In vivo 31P NMR can thus be used to measure the muscle G6P concentration after exercise.
Bloch
G
G
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
Chase
J R
JR
Avison
M J
MJ
Shulman
R G
RG
D000818:Animals; D004558:Electric Stimulation; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000032:analysis*; D007537:Isometric Contraction / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009132:Muscles / Q000737:chemistry / Q000378:metabolism*; D005082:Physical Exertion / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D005958:Glucosephosphates; D019298:Glucose-6-Phosphate