title: Membrane topology of the 22 kDa integral peroxisomal membrane protein.
abstract: In order to study the membrane topology and the possible function of the rat liver 22 kDa integral peroxisomal membrane protein (PMP 22) at a molecular level, we have cloned PMP 22 from a lambda gt11 expression library and sequenced its cDNA. Hydropathy analysis of the deduced primary structure indicates 4 putative transmembrane segments. The accessibility to exogenous aminopeptidase of PMP 22 in intact peroxisomes suggests that the N-terminus faces the cytosol. A model of the topology of PMP 22 in the peroxisomal membrane is discussed. Homology studies revealed a striking similarity with the Mpv 17 gene product. Lack of this membrane protein causes nephrotic syndrome in mice.
authors.affiliation: Institut für Biochemie I, Universität Heidelberg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Kaldi
authors.forename: P
authors.initials: P
authors.lastname: Diestelkötter
authors.forename: G
authors.initials: G
authors.lastname: Stenbeck
authors.forename: S
authors.initials: S
authors.lastname: Auerbach
authors.forename: U
authors.initials: U
authors.lastname: Jäkle
authors.forename: H J
authors.initials: HJ
authors.lastname: Mägert
authors.forename: F T
authors.initials: FT
authors.lastname: Wieland
authors.forename: W W
authors.initials: WW
authors.lastname: Just
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D007425:Intracellular Membranes / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008830:Microbodies / Q000737:chemistry*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D008565:Membrane Proteins; C065292:Mpv17 protein, mouse; D011506:Proteins; C489786:Pxmp2 protein, mouse; C079231:Pxmp2 protein, rat; D004247:DNA