title: Nitric oxide mediates inhibitory nerve input in human and canine jejunum.
abstract: BACKGROUND
  Nitric oxide (NO) may be an inhibitory neurotransmitter in the intestinal muscle. The present study examined its role in human and canine jejunum.
  
  
  METHODS
  Mechanical and intracellular electrical activity were recorded simultaneously from the circular muscle layer.
  
  
  RESULTS
  In the human jejunum, nerve stimulation inhibited mechanical activity and evoked an inhibitory junction potential that consisted of an initial fast hyperpolarization followed by a late sustained hyperpolarization. NO inhibited mechanical activity and evoked a dose-dependent hyperpolarization that mimicked the late hyperpolarization. In the canine jejunum, nerve stimulation inhibited mechanical activity and evoked an inhibitory junction potential that consisted of only a fast hyperpolarization. NG-Monomethyl-L-arginine and NG-nitro-L-arginine attenuated nerve-mediated inhibition of mechanical activity in both species. However, the effect of the synthase inhibitors on inhibitory junction potentials differed in the two species. In canine jejunum, both inhibitors reduced the amplitude of the initial fast hyperpolarization. In human jejunum, both inhibitors reduced only the late sustained hyperpolarization.
  
  
  CONCLUSIONS
  NO mediates neural inhibition in circular muscle of both human and canine jejunum through different mechanisms.
authors.affiliation: Department of Physiology and Biophysics, Mayo Clinic, Rochester, Minnesota.
authors.forename: M E
authors.initials: ME
authors.lastname: Stark
authors.forename: A J
authors.initials: AJ
authors.lastname: Bauer
authors.forename: M G
authors.initials: MG
authors.lastname: Sarr
authors.forename: J H
authors.initials: JH
authors.lastname: Szurszewski
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004285:Dogs; D004558:Electric Stimulation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007583:Jejunum / Q000294:innervation* / Q000502:physiology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D019335:Nitroarginine; D013045:Species Specificity; D019323:omega-N-Methylarginine
chemical_list: D019335:Nitroarginine; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D001120:Arginine