Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys.
Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.
Li
S L
SL
Immunopathology Laboratory, Karolinska Institute, Stockholm, Sweden.
Feichtinger
H
H
Kaaya
E
E
Migliorini
P
P
Putkonen
P
P
Biberfeld
G
G
Middeldorp
J M
JM
Biberfeld
P
P
Ernberg
I
I
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D034961:Antigens, Nuclear; D000956:Antigens, Viral / Q000378:metabolism*; D015153:Blotting, Western; D003429:Cross Reactions; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000276:immunology; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000276:immunology*; D008252:Macaca fascicularis; D009687:Nuclear Proteins / Q000032:analysis; D015302:Simian Immunodeficiency Virus / Q000276:immunology*
D015703:Antigens, CD; D034961:Antigens, Nuclear; D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D009687:Nuclear Proteins