Diphthamide synthesis in Saccharomyces cerevisiae: structure of the DPH2 gene.
A gene involved in diphthamide biosynthesis, DPH2, was cloned from Saccharomyces cerevisiae by complementation of a diphthamide mutant. DPH2 exists as a single-copy gene in the yeast genome and is located on the left arm of chromosome XI. Sequence analysis of the DPH2 locus predicts that the DPH2 gene product is a 534-amino acid (aa) protein, with a calculated M(r) of 59,772. This conclusion was supported by Northern blot analysis of the DPH2 transcript and gel analysis of the DPH2 protein overproduced in Escherichia coli. Gene disruption studies indicate that the DPH2 gene is not essential for viability of yeast. The role of DPH2 in diphthamide biosynthesis is discussed.
Mattheakis
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LC
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Sor
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Collier
R J
RJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004926:Escherichia coli; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D006639:Histidine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA
D004274:DNA, Recombinant; C083761:DPH2 protein, S cerevisiae; D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D006639:Histidine; C027527:diphthamide