Inhibition by N-ethylmaleimide of H(+)-ATPase reduces the cellular action of arginine vasopressin in cultured rat renal papillary collecting tubule cells.
We examined whether H(+)-ATPase is involved in the control of cellular action of arginine vasopressin (AVP) to produce adenosine 3', 5'-monophosphate (cAMP) and mobilize cellular free calcium ([Ca2+]i) in rat renal papillary collecting tubule cells in culture. N-Ethylmaleimide (NEM), an inhibitor of H(+)-ATPase, reduced the cellular pH (pHi) dose-dependently. AVP increased cellular cAMP production in a dose-dependent manner. 5 x 10(-6) and 1 x 10(-5) M NEM significantly diminished the AVP-induced increase in cAMP production. 1 x 10(-7) M AVP also increased [Ca2+]i from 111.2 to 189.3 nM, which was significantly reduced by NEM in a dose-dependent manner. These results indicate that H(+)-ATPase participates the cellular action of AVP mediated via the pHi control in renal papillary collecting tubule cells.
Ishikawa
S
S
Department of Medicine, Jichi Medical School, Tochigi, Japan.
Sakuma
N
N
Okada
K
K
Saito
T
T
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D003864:Depression, Chemical; D004347:Drug Interactions; D005033:Ethylmaleimide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001127:Arginine Vasopressin; D000242:Cyclic AMP; D006180:Proton-Translocating ATPases; D005033:Ethylmaleimide; D002118:Calcium