title: Detection of enterovirulent Escherichia coli associated with diarrhoea in Seville, southern Spain, with non-radioactive DNA probes.
abstract: To assess the role of diarrhoeagenic Escherichia coli in Southern Spain, faecal samples from 135 patients with diarrhoea and 40 healthy subjects from Seville, Andalusia, were investigated. In this prospective study, enterovirulent E. coli were identified by hybridisation with five non-radioactive DNA probes specific for enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC) and verocytotoxin-producing E. coli (VTEC). Probe-positive strains were isolated from four patients (3%) with diarrhoea and from none of the healthy controls. Two patients harboured ETEC and two patients had EPEC probe-positive strains in their faeces. No VTEC were isolated during this study. Salmonella spp. were the most frequently identified enteric pathogens, accounting for 10% of the cases, followed by Campylobacter jejuni (3%) and diarrhoeagenic E. coli (3%). This study indicates that enterovirulent E. coli play a modest role in the aetiology of diarrhoea among the indigenous population of Southern Spain.
authors.affiliation: Eijkman Winkler Laboratory for Medical Microbiology, University Hospital Utrecht, The Netherlands.
authors.forename: C M
authors.initials: CM
authors.lastname: Rademaker
authors.forename: L
authors.initials: L
authors.lastname: Martinez-Martinez
authors.forename: E J
authors.initials: EJ
authors.lastname: Perea
authors.forename: M
authors.initials: M
authors.lastname: Jansze
authors.forename: A C
authors.initials: AC
authors.lastname: Fluit
authors.forename: J H
authors.initials: JH
authors.lastname: Glerum
authors.forename: J
authors.initials: J
authors.lastname: Verhoef
mesh_terms: D000328:Adult; D002648:Child; D015342:DNA Probes; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D011446:Prospective Studies; D013030:Spain / Q000453:epidemiology
chemical_list: D015342:DNA Probes