Inhibition of Rev activity and human immunodeficiency virus type 1 replication by antisense oligodeoxynucleotide phosphorothioate analogs directed against the Rev-responsive element.
The interaction between the Rev protein of human immunodeficiency virus type 1 and its highly structured and conserved RNA target, the Rev-responsive element, is required for virus replication. We demonstrate that antisense oligodeoxynucleotide phosphorothioate analogs directed against the Rev-responsive element effectively inhibit Rev activity, as well as human immunodeficiency virus type 1 replication, and are candidates for antiviral therapy.
Li
G
G
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20892.
Lisziewicz
J
J
Sun
D
D
Zon
G
G
Daefler
S
S
Wong-Staal
F
F
Gallo
R C
RC
Klotman
M E
ME
D001483:Base Sequence; D002478:Cells, Cultured; D015694:Gene Products, rev / Q000037:antagonists & inhibitors*; D015658:HIV Infections / Q000517:prevention & control*; D015497:HIV-1 / Q000254:growth & development* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012367:RNA, Viral / Q000648:ultrastructure; D012045:Regulatory Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D014779:Virus Replication / Q000187:drug effects; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D016376:Oligonucleotides, Antisense; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus