Thermodependence of human lymphocyte kinetics in vitro.
Lymphocytes from 35 healthy children were grown in cultures supplied with 5-bromodeoxyuridine at different temperatures (33-40 degrees C) for 48-120 h. The mitotic index and the distribution of the percentages of cells having passed one, two, and three or more DNA replications were quantified for each experimental condition. A replication index was calculated, whose dependence on temperature was depicted in the form of two Arrhenius plots (after 72 and 96 h of culture). Results showed the thermodependence of the lymphocyte kinetics. A break point at 36.1 degrees C was found in the two Arrhenius plots. The energies of activation (kJ/mol) under the break point, 197.2, and over the break point, 100.1 (72 h) and 43.1 (96 h), were calculated.
Peteiro-Cartelle
F J
FJ
Laboratorio, Hospital Juan Canalejo, La Coruña, Spain.
D000293:Adolescent; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D004261:DNA Replication; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D008297:Male; D008940:Mitotic Index; D013696:Temperature; D013816:Thermodynamics