Cloning and sequence analysis of the phosphoprotein gene of rinderpest virus.
We have cloned several cDNAs derived from the P gene of rinderpest virus. One of these, derived from a bicistronic N-P mRNA, has been sequenced in its entirety. Sequencing of a section of the others, and comparison with the genome sequence, showed that P gene transcripts, as for other morbilliviruses, were variable; non-templated Gs could be added at a site resembling the normal stop transcription site. Primer extension analysis showed that about half the transcripts were edited. Sequences of the P, C and V proteins encoded by the normal and edited transcripts were compared with those of other morbilliviruses and with those of the more distantly related paramyxoviruses.
Baron
M D
MD
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
Shaila
M S
MS
Barrett
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005814:Genes, Viral / Q000235:genetics*; D008459:Measles virus / Q000235:genetics; D008969:Molecular Sequence Data; D010252:Paramyxoviridae / Q000235:genetics; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012302:Rinderpest virus / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D010750:Phosphoproteins; D012367:RNA, Viral; D014764:Viral Proteins