Isolation of enterohemolysin (Ehly2)-associated sequences encoded on temperate phages of Escherichia coli.
We have cloned and sequenced the enterohemolysin (ehl)-associated region from a temperate bacteriophage isolated from an Escherichia coli O26:H11 strain. Phage phi C3208 was isolated together with other temperate bacteriophages which transduce the enterohemolytic phenotype to non-hemolytic E. coli O26 strains. The nucleotide sequence of the 1245-bp phi C3208 DNA insert in plasmid pEO39, which mediates Ehly2 production in E. coli K-12, was determined and was found to be partially homologous to DNA of bacteriophage lambda but is completely unrelated to DNA sequences encoding the synthesis of Ehly1 [Stroeher et al. Gene 132 (1993), 89-94]. It was shown that part of this region can be used as an Hly2-associated specific DNA probe.
Beutin
L
L
Escherichia coli Reference Laboratory, Robert-Koch-Institut, Berlin, Germany.
Stroeher
U H
UH
Manning
P A
PA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D015342:DNA Probes; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014161:Transduction, Genetic
D015342:DNA Probes; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C066219:enterohemolysin