Requirements for Langerhans' cell depletion following in vitro exposure of murine skin to ultraviolet-B.
Langerhans' cells found within the skin and mucous membranes are critical regulators of antimicrobial and allergic responses. Therefore, the depletion of these cells following exposure of skin to solar ultraviolet radiation (UV) has direct functional consequences on immunity within this tissue. In order to understand how Langerhans' cell depletion is regulated following exposure of skin to medium-wave UV (UVB), the role of second messengers in these responses was investigated using a novel in vitro system. This was accomplished by analysing the expression of a specific marker associated with Langerhans' cells (ATPase) among the epidermal portion of cultured sections of mouse skin following treatment with inhibitors specific for second messenger components and subsequent exposure to UVB. In this study, inhibitors of guanosine triphosphate (GTP) binding proteins, H-8, pertussis toxin and cholera toxin as well as inhibitors of RNA and protein synthesis were all capable of blocking Langerhans' cell depletion in response to UVB treatment. In contrast, an inhibitor of protein kinase C (H-7) was incapable of specifically blocking depletion following treatment with this physical agent. These results suggest that Langerhans' cell depletion mediated by UVB may involve a pertussis and cholera toxin-sensitive G protein as well as de novo protein synthesis.
Burnham
K
K
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater 74078.
Pickard
S
S
Hudson
J
J
Voss
T
T
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000251:Adenosine Triphosphatases / Q000276:immunology; D000818:Animals; D002772:Cholera Toxin / Q000276:immunology; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors; D006160:Guanosine Triphosphate / Q000276:immunology; D007546:Isoquinolines / Q000276:immunology; D007801:Langerhans Cells / Q000276:immunology*; D051379:Mice; D037342:Pertussis Toxin; D010879:Piperazines / Q000276:immunology; D014176:Protein Biosynthesis; D047428:Protein Kinase Inhibitors; D012867:Skin / Q000528:radiation effects*; D014466:Ultraviolet Rays; D010566:Virulence Factors, Bordetella / Q000276:immunology
D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D010566:Virulence Factors, Bordetella; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; D006160:Guanosine Triphosphate; D002772:Cholera Toxin; D037342:Pertussis Toxin; D000251:Adenosine Triphosphatases; D019204:GTP-Binding Proteins