title: Improved bacterial hosts for regulated expression of genes from lambda pL plasmid vectors.
abstract: The construction and use of a set of Escherichia coli strains with defective lambda prophages that facilitate expression of genes cloned in lambda pL-plasmid vectors is described. These bacteria allow high and regulated expression of such genes, whereas a kanamycin-resistance marker (KmR) on the prophage allows easy identification and genetic transfer from strain to strain. Optimal conditions for examining gene expression with the pL-vector systems using these strains are discussed.
authors.affiliation: ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, MD 21702-1201.
authors.forename: T A
authors.initials: TA
authors.lastname: Patterson
authors.forename: N
authors.initials: N
authors.lastname: Costantino
authors.forename: S
authors.initials: S
authors.lastname: Dasgupta
authors.forename: D L
authors.initials: DL
authors.lastname: Court
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D003673:Defective Viruses / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids