Purification of phosphoenolpyruvate carboxylase subunits and isoforms from Vicia faba L. by preparative gel electrophoresis and their detection by enzyme-linked immunosorbent assay.
A purification procedure which yields nearly homogenous subunits of stomatal phosphoenolpyruvate carboxylase from epidermal strips of Vicia faba L. is reported. Preparative gel electrophoresis was found to be the most suitable technique for subunit purification. Denatured subunits of the stomatal enzyme in the eluate were immunologically detected by enzyme-linked immunosorbent assay (ELISA) tests. The enzyme preparation meets all requirements for its use in antibody production.
Schnabl
H
H
Institut für Landwirtschaftliche Botanik, Universität Bonn, Germany.
Klockenbring
T
T
Huhn
M
M
Schulz
M
M
D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007527:Isoenzymes / Q000302:isolation & purification*; D010730:Phosphoenolpyruvate Carboxylase / Q000302:isolation & purification*; D010944:Plants / Q000201:enzymology*
D007527:Isoenzymes; D010730:Phosphoenolpyruvate Carboxylase