Studies of DNA bending and flexibility using gel electrophoresis.
Gel electrophoretic methods have become established as primary tools in the study and elucidation of sequence-directed curvature both in free DNA and in the operator DNA of several site-specific nucleoprotein complexes. Results using them have been generally consistent with physical methods sensitive to DNA structure and conformation in those instances where direct comparisons can be made, and in a number of cases, gel methods have provided unique information not presently available from other techniques. Two basic strategies have been used: one based upon anomalous gel mobility effects; and a second based upon cyclization properties of curved DNA. Within each of these categories, various approaches have been used, some of which can lead, in favorable cases, to quantitative estimation of bending angles. In this review, the various gel-based methods that have been used to date are critically discussed and the qualitative and quantitative information that can be obtained from them is evaluated. A number of possible structural models for DNA curvature are described and a distinction is drawn between static or fixed bending and bending due to anisotropic flexibility at specific sequence loci. The importance and roles of gel electrophoretic methods in providing experimental approaches to this question are discussed. It is suggested that both static curvature and anisotropic flexibility in operator DNA may provide much of the basis for indirect readout of sequence information by specific site-binding regulatory proteins.
Harrington
R E
RE
Department of Biochemistry, University of Nevada Reno 89557.
D001483:Base Sequence; D003500:Cyclization; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004586:Electrophoresis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins / Q000378:metabolism
D009698:Nucleoproteins; D004247:DNA