Variant proteins associated with ionophore resistance in sporozoites of Eimeria tenella (Coccidia).
Protein samples prepared from sporozoites of two ionophore-sensitive strains (WIS and Penn St) and three resistant strains (FS139, FS459, and FS462) of Eimeria tenella were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), native polyacrylamide gel electrophoresis (native PAGE), or two-dimensional electrophoresis with native PAGE and SDS-PAGE. Variant proteins that might be associated with ionophore resistance were observed in resistant field strains on native PAGE. When two-dimensional electrophoresis was conducted, four over-expressed peptides with approximate molecular weights of 97, 71, 70, and 50 kDa were observed in the FS139 field strain in comparison with the WIS laboratory strain.
Zhu
G
G
Department of Poultry Science, University of Georgia, Athens 30602.
McDougald
L R
LR
D000818:Animals; D002645:Chickens; D004351:Drug Resistance; D016786:Eimeria tenella / Q000737:chemistry* / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007476:Ionophores / Q000494:pharmacology*; D010455:Peptides / Q000032:analysis; D015800:Protozoan Proteins / Q000032:analysis*
D007476:Ionophores; D010455:Peptides; D015800:Protozoan Proteins