Basic trypsin-subtilisin inhibitor from marine turtle egg white: hydrodynamic and inhibitory properties.
A basic trypsin-subtilisin inhibitor has been isolated from the egg white of marine turtle (Caretta caretta Linn.) and purified to homogeneity by gel filtration followed by ion-exchange chromatography. It has a single polypeptide chain of 117 amino acid residues, having a molecular weight of 13,600. It lacks methionine and tryptophan. Its isoelectric point is at pH 10.0 and the sedimentation coefficient (S20,w) value of 1.62 S is independent of protein concentration. It has a Stokes radius of 18.8 A, an intrinsic viscosity of 0.048 dl g-l and a diffusion coefficient of 10.17 x 10(-7) cm2 sec-1. Its fluorescence emission spectrum is similar to that of free tyrosine and the bimolecular quencing rate constant of its tyrosine residues with acrylamide is 3.15 x 10(9) M-1 sec-1. The inhibitor strongly inhibits both trypsin and subtilisin by forming enzyme-inhibitor complexes at a molar ratio of unity. The nature of inhibition toward both enzymes is not temporary. It has independent binding sites for inhibition of trypsin and subtilisin. Chemical modification with tetranitromethane suggests the presence of three tyrosine residues on the surface of the inhibitor molecule.
Sil
P C
PC
Department of Chemistry, Bose Institute, Calcutta, India.
Chaudhuri
T K
TK
Sinha
N K
NK
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004058:Diffusion; D004529:Egg White / Q000032:analysis*; D008970:Molecular Weight; D013050:Spectrometry, Fluorescence; D013381:Subtilisins / Q000037:antagonists & inhibitors*; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D014426:Turtles; D014443:Tyrosine / Q000737:chemistry; D014783:Viscosity
D000596:Amino Acids; D014361:Trypsin Inhibitors; D014443:Tyrosine; D013381:Subtilisins