title: Inhibition of human platelet glycoprotein IIB/IIIA binding to fibrinogen by tumor cell membrane proteins.
abstract: Immunochemical and functional characteristics of tumor cell membrane proteins and human platelet glycoproteins were studied. Immunoblotting revealed that membrane proteins of a cultured breast tumor cell line (BT-20) had three protein bands, which were each recognized by monoclonal antibodies to human platelet glycoprotein Ib, IIb, and IIIa, suggesting some immunochemical similarities between the tumor cell membrane proteins and platelet glycoproteins. The monoclonal antibodies failed to bind to an extract of a lung tumor cell line (A549). Neither tumor extract induced platelet aggregation. However, tumor-associated antigens isolated from the breast tumor cells markedly inhibited platelet glycoprotein IIb/IIIa binding to fibrinogen. In contrast, tumor-associated antigens from the lung tumor cells had no effect. These results suggest that tumor cells which have immunological and/or structural similarities to platelets may affect hemostasis and coagulation in vivo.
authors.affiliation: Blood Research Institute, Saint Michael's Medical Center, Newark 07102.
authors.forename: M
authors.initials: M
authors.lastname: Kamiyama
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Lynch
authors.forename: Y S
authors.initials: YS
authors.lastname: Arkel
mesh_terms: D001667:Binding, Competitive; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism*; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D010980:Platelet Membrane Glycoproteins; D005340:Fibrinogen