title: Three-dimensional reconstruction of Coxiella burnetii-infected L929 cells by high-voltage electron microscopy.
abstract: Previous examination of thin sections of L929 cells heavily infected with the Q fever Priscilla isolate by conventional transmission electron microscopy indicated that the rickettsiae resided within multiple vacuoles. The present study using high-voltage electron microscopy and three-dimensional reconstruction revealed that, in heavily infected cells, the rickettsiae, in fact, reside in one multilobed vacuole. As a result of asymmetric cell division, the multilobed vacuole containing the rickettsiae apparently segregates into one daughter cell, while the companion daughter cell emerges parasite free. This likely explains the appearance of naive uninfected cells in long-term-infected (i.e., ca. 2 years) cell populations that had not been supplemented with uninfected L929 host cells.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
authors.forename: K E
authors.initials: KE
authors.lastname: Hechemy
authors.forename: M
authors.initials: M
authors.lastname: McKee
authors.forename: M
authors.initials: M
authors.lastname: Marko
authors.forename: W A
authors.initials: WA
authors.lastname: Samsonoff
authors.forename: M
authors.initials: M
authors.lastname: Roman
authors.forename: O
authors.initials: O
authors.lastname: Baca
mesh_terms: D000818:Animals; D016997:Coxiella burnetii / Q000254:growth & development / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D007739:L Cells; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D011778:Q Fever / Q000382:microbiology / Q000473:pathology*; D014617:Vacuoles / Q000648:ultrastructure