A monoclonal anti-idiotypic antibody with an internal image of a "phosphatidylinositol-like" structure derived from anti-"phosphatidylinositol-like" IgG from the sera of patients with proliferative malignancies.
Using an enzyme-linked immunosorbent assay (ELISA) with phosphatidylinositol (PtdIns) coated on well-plates, high levels of anti-"PtdIns-like" autoantibodies (autoAbs) have been previously described in sera of cancer patients. These anti-"PtdIns-like" autoAbs were purified and injected into BALB/c mice. A monoclonal anti-idiotypic antibody (Ab2), internal image of human endogenous "PtdIns-like" structure called AIPI, was selected. Then the immunological binding in sera of cancer patients was evaluated on AIPI coated on well-plates. Using this indirect ELISA method, we found a statistically highly significant immunological binding in sera of patients with epithelial tumors, which correlated with that previously found with the PtdIns molecule coated on well-plates. Moreover, AIPI mimics an endogenous structure closely associated with PtdIns specifically encountered in epithelial proliferative disease.
Faiderbe
S
S
Laboratoire d'Immunologie et de Pathologie, Université de Bordeaux II, France.
Chagnaud
J L
JL
Geffard
M
M
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D017152:Antibodies, Antiphospholipid / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000276:immunology*; D010716:Phosphatidylinositols / Q000276:immunology*; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D017152:Antibodies, Antiphospholipid; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D010716:Phosphatidylinositols