Identification and differential expression of yeast SEC23-related gene (Msec23) in mouse tissues.
We have isolated a yeast SEC23-related clone (Msec23) from mouse fibroblast cDNA library. It has an open reading frame of 1721 bp (64% homologous to SEC23-2.3 kb) which can potentially encode a 64.7 kDa protein (61% homologous to 85.4 kDa product of SEC23). The deduced Msec23 protein (Msec23p) sequence contains three successive Ig-like domains at the N-terminus followed by amphipathic alpha-helical regions, suggesting the potential of Msec23p to interact with other protein components. Further, Msec23 is differentially expressed in mouse tissues with its high level in brain and fibroblasts.
Wadhwa
R
R
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Ibaraki, Japan.
Kaul
S C
SC
Komatsu
Y
Y
Ikawa
Y
Y
Sarai
A
A
Sugimoto
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022181:COP-Coated Vesicles; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D020690:GTPase-Activating Proteins; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D033921:Vesicular Transport Proteins
D005656:Fungal Proteins; D020690:GTPase-Activating Proteins; D011506:Proteins; D012333:RNA, Messenger; C079037:SEC23 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C079038:Sec23a protein, mouse; D033921:Vesicular Transport Proteins; D004247:DNA