Estimates of uridine diphosphate hexoses in erythrocytes: implications for galactosemia.
Impurities in a reagent dehydrogenase caused overestimates of erythrocytic uridine diphosphate glucose and accounted for clinically important differences in results between those of one group of investigators using enzymatic methods and those of two other groups using enzymatic methods, high-performance liquid chromatography, and nuclear magnetic resonance. These data have relevance for the current debate regarding the pathophysiologic changes in galactosemia.
Kirkman
H N
HN
Department of Pediatrics, University of North Carolina, Chapel Hill School of Medicine.
Clemons
E H
EH
D004340:Drug Contamination; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D005693:Galactosemias / Q000097:blood*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D014531:Uridine Diphosphate Galactose / Q000097:blood* / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000097:blood* / Q000378:metabolism; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000378:metabolism
D007202:Indicators and Reagents; D014531:Uridine Diphosphate Galactose; D005948:Glucose Dehydrogenases; D014533:Uridine Diphosphate Glucose Dehydrogenase; D007770:L-Lactate Dehydrogenase; D042843:Glucose 1-Dehydrogenase; D014532:Uridine Diphosphate Glucose