H-NMR characterization of the human myocardium myoglobin and erythrocyte hemoglobin signals.
The 1H-NMR signal of deoxy Mb provides a unique opportunity to measure tissue oxygenation in vivo. To utilize the technique for human application, however, requires a specific spectral characterization of both human Mb and erythrocyte Hb. We report that the proximal histidyl-NH signal of human deoxy Mb resonates at 80.3 ppm at 25 degrees C and maintains a 3.9 ppm separation with the corresponding Hb A signal throughout the physiological temperature range. In the particular case of the human thenar muscle, the deoxy Mb signal is clearly detectable without any interference from Hb.
Kreutzer
U
U
Biological Chemistry Department, University of California, Davis 95616-8635.
Chung
Y
Y
Butler
D
D
Jue
T
T
D000818:Animals; D001831:Body Temperature; D004912:Erythrocytes / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006736:Horses; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D010101:Oxygen Consumption
D006454:Hemoglobins; D009211:Myoglobin