title: Laboratory and field evaluation of Spherix, a formulation of Bacillus sphaericus (B-101), to control breeding of Anopheles stephensi and Culex quinquefasciatus.
abstract: Spherix, a powder formulation of Bacillus sphaericus strain B-101, serotype H5a 5b, was evaluated against larvae of Anopheles stephensi and Culex quinquefasciatus in both the laboratory and field. In laboratory tests the formulation @ 0.1 g/sq m produced 100% mortality against larvae of both mosquito species at room temperature (28-32 degrees C). The larvicidal activity of Spherix against An. stephensi @ 0.5 g/sq m persisted for over 12 weeks under laboratory conditions. Field evaluation of Spherix @ 0.25-2.0 g/sq m produced 95-100% reduction in the larval density of both An. stephensi and Culex quinquefasciatus within 48 h in different habitats, and the larvicidal activity persisted for 2-4 weeks in water habitats.
authors.affiliation: Malaria Research Centre, Delhi, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Mittal
authors.forename: T
authors.initials: T
authors.lastname: Adak
authors.forename: C P
authors.initials: CP
authors.lastname: Batra
authors.forename: V P
authors.initials: VP
authors.lastname: Sharma
mesh_terms: D000818:Animals; D000852:Anopheles; D001407:Bacillus; D003465:Culex; D007814:Larva; D009032:Mosquito Control / Q000379:methods*; D012098:Reproduction