Detection of sialic acid in trophozoites of Entamoeba histolytica (NIH:200).
In two successive experiments detection of sialic acid was achieved from trophozoites of axenically grown E. histolytica (NIH:200) using silicic acid column chromatography for the separation of sialosylated lipids and asialosylated lipids from total lipids. Sialic acid of the sialosylated lipids was detected through thiobarbituric acid assay followed by acid hydrolysis. These findings indicate that presence of sialic acid is not only a character of the cystic stage but also that of trophozoites of E. histolytica. Detection of sialic acid confirmed the electronegative charge of the surface membrane (glycocalyx) of E. histolytica trophozoites.
Nayeem
M A
MA
Department of Gastroenterology, Osmania General Hospital, Osmania Medical College, Hyderabad.
Habibullah
C M
CM
D000818:Animals; D002845:Chromatography; D004748:Entamoeba histolytica / Q000737:chemistry*; D006801:Humans; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis*
D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid