An improved dot ELISA to detect fowl adenovirus type-1 antigen.
An improved dot immunobinding assay to detect fowl adenovirus type-1 is described. The method consists of spotting of antigen on nitrocellulose membrane sheet, blocking with either 5% acetic acid or with 5% defatted milk powder and fixation of either antigen or antigen-antibody complex, with either 50% methanol or 0.25% glutaraldehyde or 0.2% tannic acid. The results revealed that fixation of both antigen and antigen-antibody complex resulted in 4-fold increase in sensitivity when acetic acid was used as blocking agent. The use of two substrates simultaneously resulted in more colour intensity and clarity than using both substrates separately.
Maiti
N K
NK
Department of Veterinary Bacteriology and Virology, Punjab Agricultural University, Ludhiana, India.
Saini
S S
SS
Singh
R
R
Oberoi
M S
MS
Sharma
S N
SN
D000257:Adenoviridae Infections / Q000175:diagnosis*; D000818:Animals; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000032:analysis*; D000259:Aviadenovirus / Q000276:immunology*; D002642:Chick Embryo; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005404:Fixatives; D011201:Poultry Diseases / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D005404:Fixatives