Particle counting by flow cytometry can determine intrinsic equilibrium constants for antibody-ligand interactions in solution.
Sykulev
Y K
YK
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Cohen
R J
RJ
Eisen
H N
HN
D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000937:Antigen-Antibody Reactions / Q000502:physiology*; D004306:Dose-Response Relationship, Immunologic; D005434:Flow Cytometry / Q000379:methods*; D006241:Haptens / Q000378:metabolism*; D066298:In Vitro Techniques
D000911:Antibodies, Monoclonal; D006241:Haptens