[Ultrastructural localization of 185 kDa and 82/41 kDa protective antigens in Plasmodium falciparum, FCC1/HN].
Plasmodium falciparum FCC1/HN-infected human erythrocytes were embedded with LR White resin at low temperature. The 185 kDa and 82/41 kDa proteins in erythrocytic stages of P. falciparum were then immunolabeled by using the protective monoclonal antibodies (McAb)F6-D3 and F6-C2 with protein A-colloidal gold probe. The electron-microscopical observation showed that the 185kDa protein recognized by McAb F6-D3 was located on the surface of free and intracellular merozoites as well as the cytoplasm, plasma membrane, and parasitophorous vacuole membrane of immature schizonts. The 82/41 kDa proteins identified by McAb F6-C2 was located within the rhoptries of immature schizonts and mature merozoites. These results demonstrated ultrastructurally that the 185 kDa and 82/41 kDa protective antigens were merozoite surface antigen and merozoite rhoptry antigens of P. falciparum FCC1/HN, respectively.
Wu
L J
LJ
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Liu
E X
EX
Zhu
Z Y
ZY
Miao
W M
WM
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis* / Q000648:ultrastructure; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology*; D016253:Microscopy, Immunoelectron; D010963:Plasmodium falciparum / Q000276:immunology / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan