Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.
Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.
Vickerman
M M
MM
Department of Cariology, School of Dentistry, University of Michigan, Ann Arbor 48109.
Heath
D G
DG
Clewell
D B
DB
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011938:Rec A Recombinases / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000235:genetics*; D013295:Streptococcus mutans / Q000235:genetics
D017931:DNA Primers; D011938:Rec A Recombinases; D015247:Deoxyribonuclease HindIII