title: Prostaglandin E2 stimulates ion transport in prairie dog gallbladder.
abstract: The effects of prostaglandins, and specifically prostaglandin E2, on gallbladder ion transport were examined in the prairie dog. Gallbladders were mounted in an Ussing chamber and baseline short-circuit current, potential difference, and tissue resistance were measured. Addition of arachidonic acid (10(-4) M, mucosal surface) produced sustained elevations in short-circuit current and potential difference (P < 0.05), with mild reductions in resistance. In a second set of tissues, indomethacin exposure (10(-6) M) resulted in a significant (P < 0.02) decrease in short-circuit current and potential difference, with an increase in resistance. Subsequent addition of prostaglandin E2 (10(-7) M, serosal surface) fully reversed these changes and led to a significant increase in short-circuit current and potential difference (P < 0.001) with a return of resistance to baseline values. These findings suggest that endogenous prostaglandins mediate gallbladder ion transport.
authors.affiliation: Research Service, Sepulveda Veterans Administration Medical Center, California.
authors.forename: K
authors.initials: K
authors.lastname: Saunders-Kirkwood
authors.forename: J A
authors.initials: JA
authors.lastname: Cates
authors.forename: J J
authors.initials: JJ
authors.lastname: Roslyn
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology*; D004848:Epithelium / Q000378:metabolism; D005704:Gallbladder / Q000378:metabolism*; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects*; D008297:Male; D012589:Sciuridae
chemical_list: D016718:Arachidonic Acid; D015232:Dinoprostone; D007213:Indomethacin