Effect of arecanut on the black mustard (Brassica niger, L.)-modulated detoxication enzymes and sulfhydryl content in the liver of mice.
The modulatory potential of arecanut, a popular masticatory, was assessed on the black mustard-induced changes in hepatic detoxication system in mice. The modulatory effect was assessed on glutathione S-transferase (GST), cytochrome b5 (Cyt. b5) and cytochrome P-450 (Cyt. P-450) and acid-soluble sulfhydryl (-SH) content. Mice were fed on either normal diet or diet containing 0.25%, 0.5% or 1% (w/w) arecanut for 45 days. During the last 10 days of treatment the feed was supplemented with 0.5% or 1% black mustard, the popular condiment. Dietary feeding of mustard could significantly enhance the studied phase I and phase II enzymes as well as -SH content in murine liver. However, black mustard-induced alterations in GST and -SH content were lower, while Cyt. b5 and Cyt. P-450 levels were much higher in mice receiving arecanut treatment than controls.
Singh
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Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Rao
A R
AR
D000818:Animals; D001114:Areca; D005260:Female; D008658:Inactivation, Metabolic; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D009149:Mustard Plant; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D013438:Sulfhydryl Compounds / Q000378:metabolism
D010936:Plant Extracts; D013438:Sulfhydryl Compounds