A quantitative radiometric assay to measure mammalian cell binding to hyphae of Candida albicans.
A rapid and reproducible assay has been developed to measure the capacity of lymphocytes to bind to Candida albicans. Lymphocytes that bound to C. albicans were either the large granular lymphocyte cell line, YT, or interleukin-2 activated lymphocytes. Lymphocyte binding was assessed as the associated radioactivity of 51Cr-labeled lymphocytes with preformed hyphae. The assay was sensitive to detection of 0.6 lymphocytes/one hyphal form at one half maximal lymphocyte binding capacity. The assay correlated well with direct microscopic assessment of lymphocyte binding to C. albicans and provided quantitative radiometric data. Although the assay was developed for the assessment of lymphocyte adhesion to C. albicans, it can be used to measure binding of other mammalian cells (e.g., polymorphonuclear leukocytes) to this fungus. In addition, the assay may be used to identify molecules involved in the adhesion of lymphocytes and other mammalian cells to C. albicans.
Forsyth
C B
CB
Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University of Chicago, Maywood, IL 60153.
Mathews
H L
HL
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002176:Candida albicans / Q000378:metabolism*; D002448:Cell Adhesion; D005260:Female; D006801:Humans; D015592:Immunoradiometric Assay; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007376:Interleukin-2