Comparative study of human and rabbit cell infection with cell-free HTLV-I.
Infection of human and rabbit cells with cell-free HTLV-I was studied by PCR analysis. Both human and rabbit PBL were infected similarly by cell-free virus of both human and rabbit cell origin. Cells were infected with the cell-free virus without prior treatment and regardless of the concentration of the culture supernatant containing the virus. Human and rabbit cell lines were also infected similarly by the cell-free virus, the proviral DNA persisting for more than two months. The culture supernatants of HTLV-I-producing cells could thus be a potential cause of laboratory infections.
Yamade
I
I
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu, Japan.
Isono
T
T
Ishiguro
T
T
Yoshida
Y
Y
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002474:Cell-Free System; D004279:DNA, Viral / Q000032:analysis; D015490:HTLV-I Infections / Q000635:transmission*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D015151:Immunoblotting; D007757:Laboratory Infection / Q000209:etiology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D011817:Rabbits; D013601:T-Lymphocytes / Q000382:microbiology; D014771:Virion / Q000472:pathogenicity* / Q000648:ultrastructure; D014776:Virus Cultivation / Q000009:adverse effects; D016662:Virus Integration
D004279:DNA, Viral