title: Extensive editing of CR2 maxicircle transcripts of Trypanosoma brucei predicts a protein with homology to a subunit of NADH dehydrogenase.
abstract: Several genes of the Trypanosoma brucei mitochondrial genome (the maxicircle) encode mRNAs that are so extensively altered by RNA editing that the gene cannot be identified by analysis of the DNA sequence. The 322-nucleotide preedited RNA of one of these genes, CR2, is converted into a 647-nucleotide transcript by the addition of 345 uridines and the deletion of 20 genomically encoded uridines. The fully edited transcript has an open reading frame that predicts a 194-amino-acid protein. This protein, which we name ND9 (NADH dehydrogenase subunit 9), has homology to a subunit of NADH dehydrogenase (respiratory complex I). Seven guide RNAs that can specify edited CR2 sequence have been identified. Steady-state levels of unedited ND9 transcripts are greater in bloodstream than in procyclic forms, but edited ND9 mRNA is present in similar abundance in both life cycle stages.
authors.affiliation: Seattle Biomedical Research Institute, Washington 98109-1651.
authors.forename: A E
authors.initials: AE
authors.lastname: Souza
authors.forename: H H
authors.initials: HH
authors.lastname: Shu
authors.forename: L K
authors.initials: LK
authors.lastname: Read
authors.forename: P J
authors.initials: PJ
authors.lastname: Myler
authors.forename: K D
authors.initials: KD
authors.lastname: Stuart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018105:DNA, Kinetoplast / Q000378:metabolism*; D016054:DNA, Protozoan / Q000378:metabolism*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D046911:Macromolecular Substances; D012333:RNA, Messenger; D009245:NADH Dehydrogenase