title: Misuse of graphical analysis in nonlinear sugar transport kinetics by Eadie-Hofstee plots.
abstract: It has become common practice to analyse the sugar transport kinetics from initial uptake rates in Saccharomyces cerevisiae cells with Eadie-Hofstee plots. These plots often demonstrate a nonlinear behaviour. They have been resolved incorrectly into two quasilinear components indicating the presence of (at least) two uptake systems or components, with Km values differing by a factor of about 10. This graphical analysis neglects the obvious additivity of the two hypothetical systems and is therefore in error. A more efficient way to determine kinetic parameters from initial uptake experiments is to use computer-assisted nonlinear regression analysis.
authors.affiliation: Department of Pharmacology and Toxicology, Philipps-University Marburg, Germany.
authors.forename: G F
authors.initials: GF
authors.lastname: Fuhrmann
authors.forename: B
authors.initials: B
authors.lastname: Völker
mesh_terms: D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D012044:Regression Analysis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D005947:Glucose