title: Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene.
abstract: Loss of histotypic organization of epithelial cells is a common feature in normal development as well as in the invasion of carcinomas. Here we show that the v-src oncogene is a potent effector of epithelial differentiation and invasiveness. MDCK epithelial cells transformed with a temperature-sensitive mutant of v-src exhibit a strictly epithelial phenotype at the nonpermissive temperature for pp60v-src activity (40.5 degrees C) but rapidly loose cell-to-cell contacts and acquire a fibroblast-like morphology after culture at the permissive temperature (35 degrees C). Furthermore, the invasiveness of the cells into collagen gels or into chick heart fragments was increased at the permissive temperature. The profound effects of v-src on intercellular adhesion were not linked to changes in the levels of expression of the epithelial cell adhesion molecule E-cadherin. Rather, we observed an increase in tyrosine phosphorylation of E-cadherin and, in particular, of the associated protein beta-catenin. These results suggest a mechanism by which v-src counteracts junctional assembly and thereby promotes invasiveness and dedifferentiation of epithelial cells through phosphorylation of the E-cadherin/catenin complex.
authors.affiliation: Institute for Cell Biology, University of Essen Medical School, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Behrens
authors.forename: L
authors.initials: L
authors.lastname: Vakaet
authors.forename: R
authors.initials: R
authors.lastname: Friis
authors.forename: E
authors.initials: E
authors.lastname: Winterhager
authors.forename: F
authors.initials: F
authors.lastname: Van Roy
authors.forename: M M
authors.initials: MM
authors.lastname: Mareel
authors.forename: W
authors.initials: W
authors.lastname: Birchmeier
mesh_terms: D000818:Animals; D015820:Cadherins / Q000032:analysis / Q000378:metabolism*; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D016391:Genes, src; D007668:Kidney; D009206:Myocardium / Q000166:cytology / Q000648:ultrastructure; D009361:Neoplasm Invasiveness; D009924:Organ Culture Techniques; D010766:Phosphorylation; D013696:Temperature; D015534:Trans-Activators; D014162:Transfection; D051176:beta Catenin
chemical_list: D015820:Cadherins; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin