title: IL-2 contributes to the IL-5 response in granulomas from mice infected with Schistosoma mansoni.
abstract: Th cells within the granulomas of murine schistosomiasis mansoni produce IL-5, which is essential for granuloma eosinophil growth and development. The mechanisms regulating granuloma IL-5 production are unknown. The granulomas also make IL-2 in small quantities. rIL-2 therapy stimulates eosinophilia and IL-5 synthesis. Therefore, we studied the effect of IL-2 on IL-5 production within the liver granulomas of murine Schistosoma mansoni. Dispersed granuloma cells and intact granulomas cultured in vitro released IL-5. Adding anti-IL-2 or anti-IL-2R to the cultures, to block IL-2 activity, significantly inhibited IL-5 production. However, supplementing the cultures with small quantities of rIL-2 markedly stimulated IL-5 release in a dose-dependent fashion. Blocking anti-IL-4 mAb had no effect. Also, granuloma T cells were isolated by FACS. These highly purified T cells produced IL-5 both in the presence and absence of plate-bound anti-CD3. Once again, the IL-5 production was dependent on IL-2. The requirement of IL-2 for normal IL-5 production was not dependent on an IL-2-induced expansion of the IL-5-producing, T lymphocyte population. Thus, IL-2 mediates T cell interactions within the granuloma that regulate granuloma IL-5 secretion.
authors.affiliation: Department of Internal Medicine, University of Iowa, Iowa City 52242.
authors.forename: A
authors.initials: A
authors.lastname: Metwali
authors.forename: D
authors.initials: D
authors.lastname: Elliott
authors.forename: R
authors.initials: R
authors.lastname: Mathew
authors.forename: A
authors.initials: A
authors.lastname: Blum
authors.forename: J V
authors.initials: JV
authors.lastname: Weinstock
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017252:CD3 Complex / Q000032:analysis; D002455:Cell Division; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006099:Granuloma / Q000378:metabolism* / Q000473:pathology; D007376:Interleukin-2 / Q000502:physiology*; D015848:Interleukin-5 / Q000096:biosynthesis*; D051379:Mice; D008808:Mice, Inbred CBA; D015375:Receptors, Interleukin-2 / Q000032:analysis; D012555:Schistosomiasis mansoni / Q000378:metabolism* / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D007376:Interleukin-2; D015848:Interleukin-5; D015375:Receptors, Interleukin-2