title: Studies on the pathophysiological mechanism of Campylobacter jejuni-induced fluid secretion in rat ileum.
abstract: Calcium has been reported to play an important role in regulating the intestinal electrolyte transport via Ca2+/calmodulin (CaM) and/or protein kinase C (PKC) systems. The role of Ca2+, CaM and PKC in the pathogenesis of Campylobacter jejuni-induced fluid accumulation was studied in vivo in ligated rat ileal loops. Calcium ionophore A23187 (5 microM) and PKC activator, phorbol-12-myristate-13-acetate (PMA, 100 micrograms kg-1) when injected alone induced fluid accumulation in the control loops. However, these modulators did not enhance further C. jejuni-induced fluid accumulation when injected along with C. jejuni live culture in the experimental loops. Both 1-verapamil (100 microM) and PKC antagonist, H-7 (15 micrograms/ml-1) significantly reduced C. jejuni-induced fluid accumulation (P < 0.001). The effect of CaM antagonist W-7 (60 microM) on C. jejuni-induced fluid secretion was not significant (P > 0.05). Our findings suggest that both Ca2+ and PKC appear to be the important second messengers involved in the stimulation of intestinal fluid accumulation in C. jejuni infection.
authors.affiliation: Department of Pediatrics, Institute of Medical Education and Research, Chandigarh, India.
authors.forename: R
authors.initials: R
authors.lastname: Kaur
authors.forename: N K
authors.initials: NK
authors.lastname: Ganguly
authors.forename: L
authors.initials: L
authors.lastname: Kumar
authors.forename: B N
authors.initials: BN
authors.lastname: Walia
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002169:Campylobacter Infections / Q000209:etiology / Q000503:physiopathology*; D016123:Campylobacter jejuni / Q000472:pathogenicity*; D003967:Diarrhea / Q000209:etiology / Q000503:physiopathology; D006801:Humans; D007082:Ileum / Q000378:metabolism*; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000502:physiology
chemical_list: D002147:Calmodulin; D011493:Protein Kinase C; D002118:Calcium