Gas chromatographic determination of trace nitrite after derivatization with ethyl 3-oxobutanoate.
A gas chromatographic method was developed for the determination of nitrite using ethyl 3-oxobutanoate as a derivatization reagent. Nitrite was reacted with ethyl 3-oxobutanoate in the presence of hydrochloric acid to form ethyl 2-hydroxyimino-3-oxobutanoate (EHOB) quantitatively. The resulting EHOB was extracted with ethyl acetate and then determined sensitively by gas chromatography with electron-capture detection. This method has been applied successfully to the determination of nitrite in river water and human saliva, with a detection limit of 2 ng/ml and recoveries of 94-100%.
Mitsuhashi
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Hyogo Prefectural Institute of Public Health, Arata-cho, Hyogo-Ku, Kobe, Japan.
D000090:Acetoacetates; D000838:Anions; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D009566:Nitrates / Q000032:analysis*; D012463:Saliva / Q000737:chemistry; D014873:Water Pollutants / Q000032:analysis*
D000090:Acetoacetates; D000838:Anions; D009566:Nitrates; D014873:Water Pollutants; C024840:ethyl acetoacetate