Synthesis of polymeric neoglycoconjugates based on N-substituted polyacrylamides.
Several types of polymeric glycoconjugates, N-substituted polyacrylamides, have been synthesized by the reaction of activated polymers with omega-aminoalkylglycosides: (i) (carbohydrate-spacer)n-polyacrylamide, 'pseudopolysaccharides'; (ii) (carbohydrate-spacer)n-phosphatidylethanolaminem-polyacrylamide, neoglycolipids, derivatives of phosphatidylethanolamine; (iii) (carbohydrate-spacer)n-biotin-polyacrylamide, biotinylated probes; (iv) (carbohydrate-spacer)n-polyacrylamide-(macroporous glass), affinity sorbents based on macroporous glass, covalently coated with polyacrylamide. An almost quantitative yield in the conjunction reaction makes it possible to insert in the conjugate a predetermined quantity of the ligand(s). Pseudopolysaccharides proved to be a suitable form of antigen for activation of polystyrene and poly(vinyl chloride) plates (ELISA) and nitrocellulose membranes (dot blot), being advantageous over traditional neoglycoproteins. Polyvalent glycolipids insert well in biological membranes: their physical properties, particularly solubility, can be changed in a desired direction. Biotinylated derivatives were used as probes for detection and analysis of lectins.
Bovin
N V
NV
Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences, Moscow, Russian Federation.
Korchagina EYu
Zemlyanukhina
T V
TV
Byramova
N E
NE
Galanina
O E
OE
Zemlyakov
A E
AE
Ivanov
A E
AE
Zubov
V P
VP
Mochalova
L V
LV
D000180:Acrylic Resins / Q000737:chemistry*; D000588:Amines; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006001:Glycoconjugates / Q000138:chemical synthesis*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D011108:Polymers; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000588:Amines; D006001:Glycoconjugates; D011108:Polymers; D000099166:Polyacrylamides