The major centromeric array of alphoid satellite DNA on the human Y chromosome is non-palindromic.
We have determined the orientation of the major centromeric alphoid array on the human Y chromosome. A PCR assay was used to analyse the vector-insert junctions of seven YAC clones previously positioned on two independent Y chromosomes. The orientation is the same at all 10 positions measured. This suggests that the alphoid array is a simple unidirectional repeat throughout and that human centromere structure thus differs from the palindromic organisation found in Schizosaccharomyces pombe.
Cooper
K F
KF
Department of Biochemistry, University of Oxford, UK.
Fisher
R B
RB
Tyler-Smith
C
C
D001483:Base Sequence; D002503:Centromere; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods; D014998:Y Chromosome
D017931:DNA Primers; D004276:DNA, Satellite