title: Comparison of the B-pentamers of heat-labile enterotoxin and verotoxin-1: two structures with remarkable similarity and dissimilarity.
abstract: We have compared the B-subunit pentamers of Escherichia coli heat-labile enterotoxin (LT) and verotoxin-1 (VT-1). The B-subunits of these bacterial toxins of the AB5 class have virtually no sequence identity and differ considerably in size (69 amino acids in VT-1 versus 103 in LT). They share a number of functional properties: pentamer formation, association with an A-subunit, binding to carbohydrate-containing lipids, and interaction with membranes. The structures of these proteins are very similar in some respects and very different in others. They can be superimposed with an rms deviation of only 1.29 A on the main chain atoms of 52 amino acids (0.98 A on 47 C alpha). Seven out of eight secondary structure elements are retained in the two toxins; only the N-terminal helix of LT is absent in VT-1. A disulfide bridge, which is essential for pentamer formation, is found in both structures, but in slightly different locations. However, the VT-1 B-subunit is much shorter on one side of the toxin, where the proposed membrane binding site of both VT-1 and LT is located. The monomer-monomer interface in the pentamer is much larger in LT than in VT-1, making the LT pentamer more stable. The central pores have a different character, and the sugar binding sites are not conserved between the toxins. The evolutionary relationship of the toxins is discussed.
authors.affiliation: BIOSON Research Institute, University of Groningen, The Netherlands.
authors.forename: T K
authors.initials: TK
authors.lastname: Sixma
authors.forename: P E
authors.initials: PE
authors.lastname: Stein
authors.forename: W G
authors.initials: WG
authors.lastname: Hol
authors.forename: R J
authors.initials: RJ
authors.lastname: Read
mesh_terms: D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000737:chemistry*; D001665:Binding Sites; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003460:Crystallization; D004220:Disulfides / Q000737:chemistry; D004355:Drug Stability; D004768:Enterotoxins / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D005690:Galactose / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D022622:Shiga Toxin 1; D013107:Sphingolipids / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D004220:Disulfides; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D005732:Gangliosides; D046911:Macromolecular Substances; D022622:Shiga Toxin 1; D013107:Sphingolipids; C027538:heat-labile enterotoxin, E coli; D005690:Galactose