title: Cloning and expression in Escherichia coli of the cDNA encoding human cardiac troponin I.
abstract: We have cloned and sequenced the human cardiac troponin I (cTnI)-encoding cDNA with the aim of expressing the cDNA in Escherichia coli. The cDNA was successfully expressed as a fusion product with beta-galactosidase and as an unfused protein. Both polypeptides were recognised by an anti-human cTnI antibody.
authors.affiliation: Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, Scotland.
authors.forename: K L
authors.initials: KL
authors.lastname: Armour
authors.forename: W J
authors.initials: WJ
authors.lastname: Harris
authors.forename: P R
authors.initials: PR
authors.lastname: Tempest
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014336:Troponin / Q000235:genetics*; D019210:Troponin I; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D014336:Troponin; D019210:Troponin I; D001616:beta-Galactosidase