title: Image analysis in the evaluation of biomaterials.
abstract: An examination regime, based on a computer-aided image analysis system, has been developed for the quantitative evaluation of the local tissue response to biomaterials. This procedure involves the immunoenzymic staining of tissue sections using monoclonal antibodies specific for certain inflammatory cell types. An avidin-biotin-horseradish peroxidase staining method is used to identify antibody binding sites and the sections are assayed using a computer-aided image analysis system. This regime facilitates the rapid and accurate measurement of 30 cell related parameters in sections stained for macrophages, polymorphonuclear leucocytes, and other cells.
authors.affiliation: Department of Clinical Engineering, University of Liverpool, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Hunt
authors.forename: D G
authors.initials: DG
authors.lastname: Vince
authors.forename: D F
authors.initials: DF
authors.lastname: Williams
mesh_terms: D000818:Animals; D001402:B-Lymphocytes; D001672:Biocompatible Materials; D006016:Glycolates; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007773:Lactates; D019344:Lactic Acid; D007958:Leukocyte Count; D008264:Macrophages; D008422:Materials Testing / Q000379:methods*; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; D019736:Prostheses and Implants; D051381:Rats; D013601:T-Lymphocytes
chemical_list: D001672:Biocompatible Materials; D006016:Glycolates; D007773:Lactates; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; C031149:glycolic acid; D019344:Lactic Acid; C033616:poly(lactide); C031892:biomer