Biochemical analysis of a mutant Tetrahymena lacking outer dynein arms.
Tetrahymena thermophila mutants homozygous for the oad mutation become nonmotile when grown at the restrictive temperature, and axonemes isolated from nonmotile mutants lack approximately 90% of their outer dynein arms. Electrophoretic analyses of axonemes isolated from nonmotile mutants (oad axonemes) indicate they contain significantly fewer of the 22 S dynein heavy chains that axonemes isolated from wild-type cells (wild-type axonemes) contain. The 22 S dynein heavy chains that remain in axonemes isolated from nonmotile, oad mutants are assembled into 22 S dynein particles that exhibit wild-type levels of ATPase activity. Two-dimensional gel electrophoresis of oad axonemes show that they are deficient in no proteins other than those proteins thought to be components of 22 S dynein. This report is the first formal proof that outer dynein arms in Tetrahymena cilia are composed of 22 S dynein.
Ludmann
S A
SA
Department of Zoology, Miami University, Oxford, Ohio 45056.
Schwandt
A
A
Kong
X
X
Bricker
C S
CS
Pennock
D G
DG
D000818:Animals; D002465:Cell Movement; D004398:Dyneins / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics; D016808:Tetrahymena thermophila / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure
D015800:Protozoan Proteins; D004398:Dyneins