Occurrence of alternatively spliced leader-delta onc-poly(A) transcripts in chicken neuroretina cells infected with Rous-associated virus type 1: implication in transduction of the c-mil/c-raf and c-Rmil/B-raf oncogenes.
We previously reported that serial passaging of Rous-associated virus type 1 in nondividing chicken embryo neuroretina cells leads to reproducible generation of acutely mitogenic retroviruses that transduced the catalytic domain of c-mil/c-raf or c-Rmil/B-raf. On the basis of structural analysis of several retroviruses, we proposed that the early step of oncogene transduction is the constitution of alternatively spliced leader-delta onc-poly(A) transcripts. Here, we show that neuroretina cells do synthesize hybrid leader-delta mil and leader-delta Rmil RNAs and that these RNAs exhibit mitogenic properties and serve as templates for the generation of transducing retorviruses.
Felder
M P
MP
Institut Curie, Centre Universitaire, Orsay, France.
Laugier
D
D
Eychene
A
A
Calothy
G
G
Marx
M
M
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001353:Avian Leukosis / Q000235:genetics* / Q000382:microbiology; D001354:Avian Leukosis Virus / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D017931:DNA Primers; D008969:Molecular Sequence Data; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019908:Proto-Oncogene Proteins c-raf; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D012160:Retina / Q000382:microbiology
D017931:DNA Primers; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D019908:Proto-Oncogene Proteins c-raf