title: Sequence-independent amplification and labeling of yeast artificial chromosomes for fluorescence in situ hybridization.
abstract: We have developed a method that allows reliable construction of high quality FISH probes from yeast artificial chromosomes (YACs) based on the separation of YACs by pulse-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can be used to localize YACs on metaphase chromosomes and also with high efficiency, in interphase nuclei.
authors.affiliation: Section of Hematology/Oncology, University of Chicago, Illinois 60637.
authors.forename: S K
authors.initials: SK
authors.lastname: Bohlander
authors.forename: R
authors.initials: R
authors.lastname: Espinosa
authors.forename: A A
authors.initials: AA
authors.lastname: Fernald
authors.forename: J D
authors.initials: JD
authors.lastname: Rowley
authors.forename: M M
authors.initials: MM
authors.lastname: Le Beau
authors.forename: M O
authors.initials: MO
authors.lastname: Díaz
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D017931:DNA Primers; D015342:DNA Probes; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D015342:DNA Probes