title: Parallel genome analysis by two-dimensional DNA typing.
abstract: By two-dimensional (2-D) DNA typing a restriction enzyme digest of genomic DNA can be resolved on the basis of both size and base-pair sequence and subsequently analysed by repeat probe hybridization to reveal sequence variants at multiple genomic sites in parallel. The system has been partly automated and allows for large-scale comparative analysis of complex genomes in a cost-effective manner.
authors.affiliation: Ingeny B. V., Leiden, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Mullaart
authors.forename: G J
authors.initials: GJ
authors.lastname: de Vos
authors.forename: G J
authors.initials: GJ
authors.lastname: te Meerman
authors.forename: A G
authors.initials: AG
authors.lastname: Uitterlinden
authors.forename: J
authors.initials: J
authors.lastname: Vijg
mesh_terms: D001331:Automation; D010582:Bacteriophage lambda / Q000235:genetics; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009693:Nucleic Acid Hybridization
chemical_list: D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes