Acceleration of anuran amphibian metamorphosis by corticotropin-releasing hormone-like peptides.
Despite substantial information on the role of the pituitary-thyroid and pituitary-interrenal axes in controlling amphibian metamorphosis, the hypothalamic hormones responsible for controlling the activity of these axes have not been identified. The mammalian thyrotropin-releasing hormone (TRH) does not regulate the thyroid axis of tadpoles; however, corticotropin releasing hormone (CRH) stimulates the release of thyrotropin from bullfrog tadpole pituitary glands in vitro and may thus function as a central regulator of the thyroid axis during metamorphosis. I tested the possibility that a CRH-like peptide is involved in controlling amphibian development by treating tadpoles of two anuran species, the western spadefoot toad Scaphiopus hammondii, and the North American bullfrog, Rana catesbeiana, with neuropeptides and monitoring their effects on metamorphosis. Injection of spadefoot toad tadpoles with ovine (o) CRH (2 micrograms/animal every other day for 3 weeks) or the amphibian CRH-like peptide sauvagine (SV) significantly decreased their time from hatching to metamorphic climax (Gosner stage 42; frontlimb emergence) and their body weight and body length at climax compared with vehicle-injected controls; whereas, TRH had no effect and arginine vasotocin produced a small but significant lengthening of the larval period but did not alter body size at climax. In an acute response experiment, S. hammondii tadpoles (in Gosner stages 36-38--late prometamorphosis) treated with oCRH or SV (2 micrograms/animal) exhibited significantly elevated whole-body thyroxine (T4) content at 2 and 6 hr after injection; whereas, treatment with TRH (2 micrograms/animal) did not significantly alter whole-body T4. R. catesbeiana tadpoles treated with oCRH or SV (surgical implantation of ELVAX pellets impregnated with 100 micrograms peptide and injections of peptides at 5 micrograms/animal once every 3 days) exhibited accelerated spontaneous and triiodothyronine (T3)-induced metamorphosis as assessed by changes in tail height, hind limb development, and body weight; TRH had no effect. Injections of a pool of antisera generated against CRH-like peptides (rat/human CRH, oCRH, SV) slowed T3-induced metamorphosis when compared with normal serum-injected controls. These results support the hypothesis that a CRH-like peptide(s) is involved in the central control of metamorphosis of anuran amphibians, and may act, at least in part, through stimulation of the thyroid axis.
Denver
R J
RJ
Department of Integrative Biology, University of California at Berkeley 94720.
D000818:Animals; D001001:Anura / Q000502:physiology*; D001824:Body Constitution; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D005260:Female; D007814:Larva / Q000187:drug effects / Q000502:physiology; D008675:Metamorphosis, Biological / Q000187:drug effects*; D009479:Neuropeptides / Q000494:pharmacology; D011892:Rana catesbeiana / Q000502:physiology; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology; D013974:Thyroxine / Q000378:metabolism; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology
D009479:Neuropeptides; D014284:Triiodothyronine; D013973:Thyrotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone; D013974:Thyroxine