title: Peripheral B cell maturation. II. Heat-stable antigen(hi) splenic B cells are an immature developmental intermediate in the production of long-lived marrow-derived B cells.
abstract: HSAhi B cells comprise 5 to 10% of adult mouse splenic B cells and are phenotypically and functionally immature. To assess their origin and relationship to mature, heat-stable Ag (HSA)lo B cells, we determined HSA and surface IgD phenotype among splenic B cells throughout development, as well as during reconstitution of lethally irradiated adults given adult B-depleted bone marrow. In each case, HSAhi splenic B cells predominate during the earliest stages of B cell genesis. Furthermore, 5-bromo-2'-deoxyuridine labeling experiments indicate rapid turnover within both the marrow and peripheral HSAhi pools, and adoptive transfer studies show that peripheral HSAhi splenic B cells differentiate to HSAlo within 4 days. Finally, splenic HSAhi B cells reconstitute both primary and memory humoral responses. Together, these data indicate that splenic B cells in the HSAhi subset are an intermediate maturational stage in the adult periphery.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-6082.
authors.forename: D M
authors.initials: DM
authors.lastname: Allman
authors.forename: S E
authors.initials: SE
authors.lastname: Ferguson
authors.forename: V M
authors.initials: VM
authors.lastname: Lentz
authors.forename: M P
authors.initials: MP
authors.lastname: Cancro
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D001854:Bone Marrow Cells; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology*; D006358:Hot Temperature; D007072:Immunoglobulin D / Q000032:analysis; D016219:Immunotherapy, Adoptive; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D017455:Receptors, IgE / Q000032:analysis; D013154:Spleen / Q000166:cytology*
chemical_list: D000943:Antigens, Differentiation; D007072:Immunoglobulin D; D011947:Receptors, Antigen, B-Cell; D017455:Receptors, IgE