title: Ontogeny of Ia messenger RNA in the mouse small intestinal epithelium is modulated by age of weaning and diet.
abstract: BACKGROUND
  Exogenous antigenic peptides are presented to T cells by class II major histocompatibility complex (Ia) molecules on the surface of antigen-presenting cells. Class II-associated invariant chain (Ii) is also required for effective antigen presentation. Because messenger RNAs (mRNAs) for Ii chain and for class II I-A beta chain appear in the mouse intestinal epithelium after weaning, experiments were conducted to test the effect of age of weaning and diet on the appearance of Ia and Ii mRNA.
  
  
  METHODS
  Four litters were split at day 17; one half was weaned and the other remained with the mother until day 24. On day 23, 25, 27, and 29, enterocytes were isolated from full-length small intestine by vascular perfusion with 30 mmol/L ethylenediaminetetraacetic acid, and the RNA was extracted.
  
  
  RESULTS
  Appearance of Ii and I-A beta was significantly delayed by late weaning, as judged by RNA hybridization blots (Ii chain) and complementary DNA amplification (I-A beta chain). In mice on elemental diets, the appearance of Ii and I-A beta chain was delayed compared with littermates reared on standard chow. Ii mRNA failed to appear in mice maintained on the elemental diet by day 40, despite normal growth.
  
  
  CONCLUSIONS
  Appearance of mRNA for both Ia and Ii depends on the introduction of a complex diet and not the "stress" of weaning or elimination of breast milk. Introduction of foreign dietary antigens or development of an altered intestinal flora may contribute to this process.
authors.affiliation: Mucosal Immunology Laboratory, Massachusetts General Hospital, Boston.
authors.forename: I R
authors.initials: IR
authors.lastname: Sanderson
authors.forename: A J
authors.initials: AJ
authors.lastname: Ouellette
authors.forename: E A
authors.initials: EA
authors.lastname: Carter
authors.forename: P R
authors.initials: PR
authors.lastname: Harmatz
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D001683:Biological Clocks; D004032:Diet; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000378:metabolism; D015151:Immunoblotting; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000008:administration & dosage / Q000494:pharmacology; D007421:Intestine, Small / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014886:Weaning
chemical_list: D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D007371:Interferon-gamma