title: Phenotypic and cytogenetic characterization of a human corpus cavernosum cell line (DS-1).
abstract: We report here the establishment and characterization of a corpus cavernosum cell line (DS-1) from human penile tissue. This is the first cell line of its type derived from cavernosum tissue. DS-1 cells have become immortalized in culture, and show growth in monolayers. These cells have a doubling time of about 45 h in in vitro culture. Cytogenetic analysis by G-banding demonstrated a diploid karyotype with a model chromosome number of 46. The chromosome constitution of DS-1 cells was found to be male (XY), in 28/30 cells scored. Two of the 30 cells showed an extra structurally rearranged "marker" chromosome, that appeared to be a derivative of chromosome 18 with excessive chromosome on the short arm. Ploidy analysis revealed that the majority of DS-1 cells had a DNA index of one. About 35% cells were found to be in G-1 phase and 52% cells in S phase. Light and electron microscopy of DS-1 cells and original penile tissue showed typical characteristics of this tissue. Immunocytochemistry studies using antibodies to smooth muscle actin, desmin, vimentin and cytokeratin (LP34, CAM5.2) showed that the DS-1 cell line had predominantly smooth muscle cells, as these cells were positive for smooth muscle actin, desmin and vimentin.
authors.affiliation: Department of Urology, University of California, San Francisco.
authors.forename: R
authors.initials: R
authors.lastname: Dahiya
authors.forename: S
authors.initials: S
authors.lastname: Sikka
authors.forename: W J
authors.initials: WJ
authors.lastname: Hellstrom
authors.forename: S W
authors.initials: SW
authors.lastname: Hayward
authors.forename: P
authors.initials: P
authors.lastname: Narayan
authors.forename: E A
authors.initials: EA
authors.lastname: Tanagho
authors.forename: T F
authors.initials: TF
authors.lastname: Lue
mesh_terms: D002455:Cell Division; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000032:analysis; D007172:Erectile Dysfunction / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D010413:Penis / Q000166:cytology*; D010641:Phenotype; D011003:Ploidies
chemical_list: D003598:Cytoskeletal Proteins