Rapid identification of Triticeae genotypes from single seeds using the polymerase chain reaction.
An easy and quick protocol has been developed for DNA analysis via PCR. Single cereal endosperm or small leaf pieces can be separately processed in several PCR reactions. The resultant PCR patterns are equivalent to those obtained with standard DNA extraction protocols using either specific or random primers. Intra- and inter-specific variability can be detected. This method allows the analysis of a large number of individuals in early stages prior to the plant sowing.
Benito
C
C
Department of Genetics, Faculty of Biology, University Complutense, Madrid, Spain.
Figueiras
A M
AM
Zaragoza
C
C
Gallego
F J
FJ
de la Peña
A
A
D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000502:physiology; D014908:Triticum / Q000235:genetics*
D004247:DNA