title: Expression cDNA cloning of a transforming gene encoding the wild-type G alpha 12 gene product.
abstract: Using an expression cDNA cloning approach, we examined human tumor cell lines for novel oncogenes that might evade detection by conventional techniques. We isolated a transforming sequence that was highly efficient in transforming NIH 3T3 mouse fibroblasts. DNA sequence analysis identified the gene as the human homolog of a recently cloned alpha subunit of mouse GTP-binding protein G alpha 12. NIH 3T3 cells transfected with G alpha 12 cDNA grew in soft agar and were tumorigenic in nude mice. There were no apparent mutations in the cloned cDNA in comparison with a G alpha 12 cDNA clone isolated from a normal human epithelial cell library, implying that overexpression alone was sufficient to cause NIH 3T3 cell transformation. The observed altered growth properties mediated by G alpha 12 showed a certain degree of dependency on serum factors, and its mitogenic potential was also potently inhibited by suramin treatment.
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
authors.forename: A M
authors.initials: AM
authors.lastname: Chan
authors.forename: T P
authors.initials: TP
authors.lastname: Fleming
authors.forename: E S
authors.initials: ES
authors.lastname: McGovern
authors.forename: M
authors.initials: M
authors.lastname: Chedid
authors.forename: T
authors.initials: T
authors.lastname: Miki
authors.forename: S A
authors.initials: SA
authors.lastname: Aaronson
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000502:physiology; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D017386:Sequence Homology, Amino Acid; D013498:Suramin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D013498:Suramin; D004247:DNA; D019204:GTP-Binding Proteins