Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.
The effects of 30 type 1 and of 2 (ricin and volkensin) type 2 ribosome-inactivating proteins (RIPs) on Escherichia coli and Agrobacterium tumefaciens cell-free translation systems were compared with the effects on a rabbit reticulocyte translation system. The depurinating activity of RIPs on E. coli ribosomes was also evaluated. Only six type 1 RIPs inhibited endogenous mRNA-directed translational activity of E. coli lysates, with submicromolar 50% inhibitory concentrations. Four RIPs had similar activities on poly(U)-directed phenylalanine polymerization by E. coli ribosomes, and three RIPs inhibited poly(U)-directed polyphenylalanine synthesis by A. tumefaciens ribosomes, with submicromolar 50% inhibitory concentrations.
Girbés
T
T
Departmento de Bioquimica, Biologia Molecular y Fisiologia, Facultad de Ciencias, Universidad de Valladolid, Spain.
Barbieri
L
L
Ferreras
M
M
Arias
F J
FJ
Rojo
M A
MA
Iglesias
R
R
Alegre
C
C
Escarmis
C
C
Stirpe
F
F
D016960:Agrobacterium tumefaciens / Q000187:drug effects* / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012335:RNA, Ribosomal / Q000737:chemistry; D012338:RNA, Ribosomal, 23S / Q000737:chemistry; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects*; D013045:Species Specificity
D010940:Plant Proteins; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S; C065096:RNA, ribosomal, 26S; D054789:Ribosome Inactivating Proteins, Type 1; C012857:crotin II protein, Croton tiglium; D006026:Glycoside Hydrolases