Evidence for the in-vitro de-novo synthesis of immunoglobulin and a previously undescribed 17 kDa protein (TEP-2) by the mucosa of the fallopian tube.
De-novo synthesis and secretion of proteins by short-term explants of matched Fallopian tube mucosa and endometrium were studied using radiolabelled L-[35S]methionine and [3H]glucosamine. To compare directly each anatomical site of the Fallopian tube and endometrium from the same source, newly synthesized proteins were separated on one-dimensional polyacrylamide gel electrophoresis and examined by auto-radiography. De-novo synthesis of two protein bands provisionally designated as tubal epithelial protein 1 (TEP-1) and tubal epithelial protein 2 (TEP-2), was observed in explants of the Fallopian tube mucosa obtained from each anatomical site throughout the ovarian cycle (n = 20). TEP-2 was not apparent in tubal mucosa obtained from post-menopausal women (n = 5). De-novo synthesis of TEP-1 and TEP-2 was not apparent in autoradiographs of radiolabelled proteins from short-term explants of endometrium. From the autoradiographs the molecular mass of TEP-1 and TEP-2 was calculated to be 25 kDa and 17 kDa, respectively. Incorporation of glucosamine into newly synthesized protein occurred in TEP-2 but not TEP-1. TEP-1 was observed to be immunochemically identical to immunoglobulin kappa light chains.
Maguiness
S D
SD
Academic Unit of Obstetrics and Gynaecology, London Hospital Medical College, Royal London Hospital Trust, Whitechapel, UK.
Shrimanker
K
K
Djahanbakhch
O
O
Teisner
B
B
Grudzinskas
J G
JG
D000328:Adult; D000368:Aged; D001345:Autoradiography; D015153:Blotting, Western; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000378:metabolism; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D008875:Middle Aged; D008970:Molecular Weight; D009092:Mucous Membrane / Q000378:metabolism; D017698:Postmenopause; D017697:Premenopause; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification
D007136:Immunoglobulins; D011506:Proteins