title: The inhibition by fatty acids of receptor-mediated calcium movements in Jurkat T-cells is due to increased calcium extrusion.
abstract: Numerous studies on the molecular basis of the mechanism of action of fatty acids have demonstrated their action in cell signaling and particularly on the regulation of cytosolic Ca2+ concentration. Stimulation of Jurkat T cells with CD3 monoclonal antibody results in an increase of intracellular calcium concentration, [Ca2+]i due both to a release of Ca2+ from intracellular stores and a Ca2+ influx. [Ca2+]i increase represents a dynamic balance between Ca2+ influx and efflux. Fatty acids, either saturated (C14:0), monounsaturated (C16:1), or polyunsaturated, belonging to the C18 and the C20 series induce a marked decrease of CD3-induced [Ca2+]i rise. This property of fatty acid is independent of the position of the carbon-carbon double bond but specific of the cis stereoisomeric form. Fatty acids does not block CD3-induced signals but greatly stimulates the Ca2+ extrusion process probably by activating the plasma membrane Ca(2+)-ATPase. This was documented by the observation that fatty acids, reduced to the same extent as [Ca2+]i, elicited either with CD3 monoclonal antibody the calcium ionophore ionomycin or the Ca(2+)-ATPase inhibitor thapsigargin.
authors.affiliation: Interactions Cellularies en Immunologie et Immunopathologie, Institut National de la Santé et de la Recherche Médicale U343, Nice, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Breittmayer
authors.forename: C
authors.initials: C
authors.lastname: Pelassy
authors.forename: J L
authors.initials: JL
authors.lastname: Cousin
authors.forename: A
authors.initials: A
authors.lastname: Bernard
authors.forename: C
authors.initials: C
authors.lastname: Aussel
mesh_terms: D000911:Antibodies, Monoclonal / Q000502:physiology*; D017252:CD3 Complex / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002460:Cell Line; D005227:Fatty Acids / Q000494:pharmacology*; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation; D010718:Phosphatidylserines / Q000096:biosynthesis; D015398:Signal Transduction / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D005227:Fatty Acids; D010718:Phosphatidylserines; D013729:Terpenes; D015759:Ionomycin; D019284:Thapsigargin; D000252:Calcium-Transporting ATPases; D002118:Calcium