Specific in vitro labeling of cells with a fluorine-19 probe encapsulated in antibody-targeted liposomes: a F-19 NMR spectroscopy study.
Liposomes containing dexamethasone phosphate (DMp) were covalently coupled to protein A and then incubated with murine L929 fibroblast and RDM4 thymoma cells in the presence of monoclonal antibodies specific for the major histocompatibility complex. The detection of the specific F-19 labeling of cells is rapid (minutes). Such a strategy might be useful to study the kinetics of internalization processes of bound liposomes on cultured living cells.
Vion-Dury
J
J
Centre de Résonance Magnétique Biologique et Médicale, (URA CNRS 1186), Faculté de Médecine, Marseille, France.
Machy
P
P
Confort-Gouny
S
S
Leserman
L
L
Cozzone
P J
PJ
D000818:Animals; D000911:Antibodies, Monoclonal; D003907:Dexamethasone; D005461:Fluorine; D066298:In Vitro Techniques; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013205:Staphylococcal Protein A; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D008081:Liposomes; D013205:Staphylococcal Protein A; D005461:Fluorine; D003907:Dexamethasone