title: Repression of stromelysin metalloprotease expression in rat fibrosarcoma cells by dimethylsulfoxide.
abstract: Metalloproteases are implicated in conferring invasive properties to tumor cells. We show here that treatment of ras-oncogene-transformed rat fibroblasts with dimethylsulfoxide (DMSO) results in a reversible decrease in stromelysin mRNA. Furthermore, stromelysin expression was found to be repressed by DMSO, but not by glucocorticoid hormone, in a fibrosarcoma cell line showing low AP-1 (fos/jun) transcription factor activity. In two fibrosarcoma cell lines which express high levels of stromelysin and low levels of 68 kDa type IV collagenase, the DMSO-induced decrease in stromelysin expression was paralleled by a decreased invasive propensity.
authors.affiliation: Department of Oncology, Radiumhemmet, Karolinska Institute and Hospital, Stockholm, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Popowicz
authors.forename: G
authors.initials: G
authors.lastname: Engel
authors.forename: H
authors.initials: H
authors.lastname: Marshall
authors.forename: S
authors.initials: S
authors.lastname: Linder
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004794:Enzyme Repression / Q000187:drug effects; D005354:Fibrosarcoma / Q000201:enzymology* / Q000473:pathology; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000187:drug effects*; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D004121:Dimethyl Sulfoxide