Evaluation of enzyme immunoassay performance characteristics--phencyclidine example.
Four reagent formulations (three provided by a manufacturer; one prepared in-house by mixing equal volumes of two commercial reagents) are used for the assay of phencyclidine (PCP) in urine samples. Performance characteristics evaluated included assay precision and sensitivity at and near the assay cutoff concentration. Data resulting from the reagent prepared in-house are better than those using then commercially available formulations, and are comparable with those obtained using the recently available new commercial formulation.
Fyfe
M J
MJ
Environmental Chemical Corporation, Birmingham, AL.
Chand
P
P
McCutchen
C
C
Long
J S
JS
Walia
A S
AS
Edwards
C
C
Liu
R H
RH
D017248:Enzyme Multiplied Immunoassay Technique; D007202:Indicators and Reagents / Q000737:chemistry; D010622:Phencyclidine / Q000652:urine*; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents; D010622:Phencyclidine