title: Structure of hydrated oligonucleotides studied by in situ scanning tunneling microscopy.
abstract: We have used the scanning tunneling microscope (STM) to image several synthetic oligonucleotides adsorbed onto a positively charged Au(111) electrode. The molecules were deposited and imaged in aqueous electrolyte under potential control, a procedure that eliminated the problem of the substrate artifacts that had limited some previous STM studies. Experiments were carried out with two types of single-stranded molecules (11 and 20 bases long) and three types of double-stranded molecules (20 and 61 base pairs and 31 bases with 25 bases paired and 6-base "sticky" ends). The molecules lie along symmetry directions on the reconstructed (23 x square root of 3) gold surface, and length measurements indicate that they adopt simple base-stacked structures. The base stacking distances are, within experimental uncertainty, equal to the 0.33 nm measured for polymeric aggregates of stacked purines by direct imaging in identical conditions. The images show features consistent with helical structures. Double helices have a major-groove periodicity that is consistent with a 36 degrees twist. The single helices appear to be more tightly twisted. A simple tunneling model of STM contrast generates good agreement between measured and calculated images.
authors.affiliation: Department of Physics and Astronomy, Arizona State University, Tempe 85287.
authors.forename: T W
authors.initials: TW
authors.lastname: Jing
authors.forename: A M
authors.initials: AM
authors.lastname: Jeffrey
authors.forename: J A
authors.initials: JA
authors.lastname: DeRose
authors.forename: Y L
authors.initials: YL
authors.lastname: Lyubchenko
authors.forename: L S
authors.initials: LS
authors.lastname: Shlyakhtenko
authors.forename: R E
authors.initials: RE
authors.lastname: Harrington
authors.forename: E
authors.initials: E
authors.lastname: Appella
authors.forename: J
authors.initials: J
authors.lastname: Larsen
authors.forename: A
authors.initials: A
authors.lastname: Vaught
authors.forename: D
authors.initials: D
authors.lastname: Rekesh
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000648:ultrastructure*; D016252:Microscopy, Scanning Tunneling / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA