Protective activity of anti-exotoxin A monoclonal antibody against mice infected with toxin-producing Pseudomonas aeruginosa.
The neutralizing and protective effect of murine monoclonal antibody (MAb) 3C7 (IgG1) against Pseudomonas aeruginosa exotoxin A was examined in an experimental mouse model of infection with exotoxin A-producing strains. Treatment with MAb 3C7 blocked the reduction of functional elongation factor 2 (EF-2) in the liver of mice but could not clear the bacteria. Administration of gentamicin caused bacteria to be cleared but did not block reduction of hepatic EF-2 level. Treatment with either MAb 3C7 or gentamicin individually did not prolong time to death; however, the combined therapy with both MAb 3C7 and gentamicin cleared bacteria and blocked the reduction of hepatic EF-2 level, resulting in a significant increase in the survival rate of mice. These results suggest that anti-exotoxin A MAbs show effectiveness against pseudomonal infection caused by exotoxin A-producing strains.
Kohzuki
T
T
Research Laboratories, Sumitomo Pharmaceuticals Ltd., Osaka, Japan.
Eguchi
Y
Y
Kato
M
M
Irie
K
K
Ohtsuka
H
H
Higuchi
A
A
Noguchi
H
H
D036002:ADP Ribose Transferases; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001427:Bacterial Toxins / Q000276:immunology; D003131:Combined Modality Therapy; D005098:Exotoxins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D005839:Gentamicins / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000032:analysis; D011552:Pseudomonas Infections / Q000628:therapy*; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000472:pathogenicity; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D005098:Exotoxins; D005839:Gentamicins; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A