A proteoglycan form of macrophage colony-stimulating factor that binds to bone-derived collagens and can be extracted from bone matrix.
We previously found that the human osteoblastic cell line MG-63 produces two molecular types of macrophage colony-stimulating factor (M-CSF). One is an 85-kD M-CSF, and the other is a proteoglycan form of M-CSF (PG-M-CSF) that has a binding affinity to type V collagen. The latter type of M-CSF showed dose-dependent binding to wells coated by pepsin-extracted bone collagens, whereas the 85-kD M-CSF did not. Immunoblot analysis of urea-extracted bone M-CSF revealed the presence of PG-M-CSF. PG-M-CSF contained in bone matrix may have physiological importance in the bone metabolism.
Ohtsuki
T
T
Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Suzu
S
S
Hatake
K
K
Nagata
N
N
Miura
Y
Y
Motoyoshi
K
K
D001857:Bone Matrix / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000302:isolation & purification / Q000378:metabolism*; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D010006:Osteoblasts; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification; D011509:Proteoglycans / Q000302:isolation & purification / Q000378:metabolism*; D014508:Urea
D017077:Culture Media, Conditioned; D011506:Proteins; D011509:Proteoglycans; D016173:Macrophage Colony-Stimulating Factor; D014508:Urea; D003094:Collagen