Matrix-assisted laser desorption mass spectrometry of cytochromes P450.
Matrix-assisted laser desorption ionization on a time-of-flight (MALDI/TOF) mass spectrometer provides a rapid and accurate method for molecular weight determination of hydrophobic cytochrome P450 proteins (P450s). A mass accuracy of 0.075% was achieved by analysis of dialyzed P450 2B1 using bovine serum albumin (BSA) as an internal standard. The measured mass of cytochrome P450 2B2 with MALDI/TOF resulted in an average molecular weight which was higher than reported values. Immobilization procedures proved extremely effective on samples containing high concentrations of phosphate buffers.
Lewis
S
S
Finnigan MAT, San Jose, CA 95134-1991.
Korsmeyer
K K
KK
Correia
M A
MA
D000818:Animals; D019362:Cytochrome P-450 CYP2B1; D003577:Cytochrome P-450 Enzyme System; D007834:Lasers; D008297:Male; D013058:Mass Spectrometry; D008970:Molecular Weight; D010088:Oxidoreductases; D051381:Rats; D017207:Rats, Sprague-Dawley
D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019362:Cytochrome P-450 CYP2B1