c-fos gene expression in cell revertants from a transformed to a pseudonormal phenotype.
c-fos gene expression in two types of mouse sarcoma cells of spontaneous origin and in revertants to pseudonormal phenotype has been investigated. In the latter cells the content of c-fos mRNA is similar to that in normal fibroblasts. Activity of transcription factors interacting with the regulatory elements, SRE, DSE and TRE, in the c-fos promoter do not correlate with the c-fos mRNA concentration. However, experiments with cells transformed with the indicator plasmid, fos-CAT, showed that the 600 bp c-fos promoter region provides the chloramphenicol acetyltransferase activity correlating with c-fos mRNA expression in cell revertants to a pseudonormal phenotype.
Lavrovsky
Y V
YV
Institute of Bioorganic Chemistry, Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk.
Svinarchuk
F P
FP
Lavrovsky
V A
VA
Yefremov
Y R
YR
Vlassov
V V
VV
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004277:DNA, Single-Stranded; D005347:Fibroblasts; D015870:Gene Expression; D016762:Genes, fos; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
D004277:DNA, Single-Stranded; D012333:RNA, Messenger; D014157:Transcription Factors; D015500:Chloramphenicol O-Acetyltransferase