title: Crystal structure of glucose oxidase from Aspergillus niger refined at 2.3 A resolution.
abstract: Glucose oxidase (beta-D-glucose: oxygen 1-oxidoreductase, EC 1.1.3.4) is an FAD-dependent enzyme that catalyzes the oxidation of beta-D-glucose by molecular oxygen. The crystal structure of the partially deglycosylated enzyme from Aspergillus niger has been determined by isomorphous replacement and refined to 2.3 A resolution. The final crystallographic R-value is 18.1% for reflections between 10.0 and 2.3 A resolution. The refined model includes 580 amino acid residues, the FAD cofactor, six N-acetylglucosamine residues, three mannose residues and 152 solvent molecules. The FAD-binding domain is topologically very similar to other FAD-binding proteins. The substrate-binding domain is formed from non-continuous segments of sequence and is characterized by a deep pocket. One side of this pocket is formed by a six-stranded antiparallel beta-sheet with the flavin ring system of FAD located at the bottom of the pocket on the opposite side. Part of the entrance to the active site pocket is at the interface to the second subunit of the dimeric enzyme and is formed by a 20-residue lid, which in addition covers parts of the FAD-binding site. The carbohydrate moiety attached to Asn89 at the tip of this lid forms a link between the subunits of the dimer.
authors.affiliation: GBF (Gesellschaft für Biotechnologische Forschung), Department of Molecular Structure, Braunschweig, Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Hecht
authors.forename: H M
authors.initials: HM
authors.lastname: Kalisz
authors.forename: J
authors.initials: J
authors.lastname: Hendle
authors.forename: R D
authors.initials: RD
authors.lastname: Schmid
authors.forename: D
authors.initials: D
authors.lastname: Schomburg
mesh_terms: D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology*; D001665:Binding Sites; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry*; D006031:Glycosylation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
chemical_list: D005182:Flavin-Adenine Dinucleotide; D005949:Glucose Oxidase