Identification of nodSUIJ genes in Nod locus 1 of Azorhizobium caulinodans: evidence that nodS encodes a methyltransferase involved in Nod factor modification.
The Azorhizobium caulinodans strain ORS571 nodulation genes nodSUIJ were located downstream from nodABC. Complementation data and transcriptional analysis suggest that nodABCSUIJ form a single operon. Mutants with Tn5 insertions in the genes nodS, nodU, and nodJ were delayed in nodulation of Sesbania rostrata roots and stems. The NodS amino acid sequences of ORS571, Bradyrhizobium japonicum, and Rhizobium sp. strain NGR234, contain a consensus with similarity to S-adenosylmethionine (SAM)-utilizing methyltransferases. A naringenin-inducible nodS-dependent protein of approximately 25 kDa could be cross-linked to radiolabelled SAM. By applying L-[methyl-3H]-methionine in vivo, Nod factors of ORS571, known to be N-methylated, could be labelled in wild type and nodU mutants but not in nodS mutants. Therefore, we propose that NodS is a SAM-utilizing methyltransferase involved in Nod factor synthesis.
Geelen
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Laboratorium voor Genetica, Universiteit Gent, Belgium.
Mergaert
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P
Geremia
R A
RA
Goormachtig
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S
Van Montagu
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Holsters
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D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D019878:Carboxyl and Carbamoyl Transferases; D002352:Carrier Proteins / Q000235:genetics*; D016384:Consensus Sequence; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins; D008780:Methyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D012230:Rhizobiaceae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; C065618:nodI protein, Bacteria; C083534:nodJ protein, Azorhizobium caulinodans; C073986:nodS protein, Rhizobiaceae; D008780:Methyltransferases; D019878:Carboxyl and Carbamoyl Transferases; C073987:nodU protein, Bacteria