Chemical structure of lipid A from Porphyromonas (Bacteroides) gingivalis lipopolysaccharide.
The novel chemical structure of Porphyromonas (Bacteroides) gingivalis strain 381 lipid A was determined to be a glucosamine beta-(1-6) disaccharide 1-monophosphate acylated by 3-hydroxy-15-methylhexadecanoic acid and 3-hexadecanoyloxy-15-methylhexadecanoic acid at the 2- and 2'-positions, respectively. The absence of ester-linked phosphate at the 4'-position and fatty acids at the 3- and 3'-positions, and the presence of fatty acids possessing 16-17 carbon atoms are unique features, differentiating the lipid A from enterobacterial and other lipid As. These structural features may be related to its low endotoxic activity. Furthermore, P. gingivalis lipid A as well as its LPS stimulated the splenocytes from C3H/HeN and C3H/HeJ mice.
Ogawa
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Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
D000818:Animals; D002240:Carbohydrate Sequence; D005227:Fatty Acids / Q000032:analysis; D008050:Lipid A / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016966:Porphyromonas gingivalis / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides