An inexpensive computer-aided morphometric device for the analysis of dissociated brain cell cultures.
Morphometric analysis of brain cells growing in dissociated cell culture is a promising approach in neuroscience research. Here we present new software written in Turbo PASCAL which has the advantage of requiring only a minimum in equipment accessible in most neuroscience labs, i.e., a microscope with a camera lucida attachment and a personal computer. The camera lucida is used to superimpose the pictures from the microscope and the computer screen. With the aid of a mouse, cells are drawn to the computer screen. The program counts pixels and calculates morphometric data including the area occupied by the cell soma as well as number, length and arborization of the processes. The program may be obtained from the authors upon request.
Kolbinger
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W
Department of Anatomy and Cell Biology, University of Ulm, Germany.
Warchol
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W
D000818:Animals; D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation; D012984:Software