title: Crystallization and preliminary crystallographic characterization of the copper-containing amine oxidase from pea seedlings.
abstract: The copper-containing amine oxidase from pea seedlings has been crystallized using lithium sulfate as precipitant at pH 5.2. The unit cell is orthorhombic, space group P2(1)2(1)2(1), with dimensions a = 89.3 A, b = 113.4 A, c = 199.0 A. The mass of the asymmetric unit is 131(+/- 13) kDa, consistent with independent evidence that the molecule has two approximately 66 kDa subunits. The crystals diffract to 2.5 A in a synchrotron X-ray beam.
authors.affiliation: Department of Inorganic Chemistry, University of Sydney, N.S.W., Australia.
authors.forename: V
authors.initials: V
authors.lastname: Vignevich
authors.forename: D M
authors.initials: DM
authors.lastname: Dooley
authors.forename: J M
authors.initials: JM
authors.lastname: Guss
authors.forename: I
authors.initials: I
authors.lastname: Harvey
authors.forename: M A
authors.initials: MA
authors.lastname: McGuirl
authors.forename: H C
authors.initials: HC
authors.lastname: Freeman
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry*; D003460:Crystallization; D007887:Fabaceae / Q000201:enzymology*; D010946:Plants, Medicinal; D014961:X-Ray Diffraction
chemical_list: D006631:Amine Oxidase (Copper-Containing)