Induction of G-bands on Anguilla anguilla chromosomes by the restriction endonucleases HaeIII, HinfI, and MseI.
Fixed metaphase chromosomes of the eel Anguilla anguilla were treated with the restriction enzymes HaeIII, HinfI, and MseI. All three restriction endonucleases are capable of inducing a G-band-like pattern if the incubation time and enzyme concentration are controlled. The results are discussed in relation to the main factors involved in the appearance of serial G-bands. Chromatin organization, rather than DNA composition, seems to account for the interstitial G-band pattern exhibited by these eel chromosomes.
Viñas
A
A
Departamento de Biología Fundamental, Universidad de Santiago de Compostela, Lugo, Spain.
Gómez
C
C
Martínez
P
P
Sánchez
L
L
D000811:Anguilla; D000818:Animals; D002871:Chromosome Banding; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005260:Female; D007621:Karyotyping; D008297:Male
C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases