3D reconstruction of biological objects from sequential image planes--applied on cerebral cortex from cat.
A prism of cat cerebral cortex was reconstructed with a method for three-dimensional (3D) representation of biological objects. A series of 918 semithin sections were digitized into an image analysis system. The images were aligned and analyzed, and a data base with the coordinates and a classification of the cells was created. The data base (i.e., the cortical prism) was visualized in a 3D graphic terminal, and parameters such as columnar and lamellar organization, clustering, and cell density were analyzed. A neuronal perikaryon and its neurites was reconstructed and shown together with the cortical prism.
Skoglund
T
T
Department of Anatomy, Medical Faculty, Göteborg University, Sweden.
Pascher
R
R
Berthold
C H
CH
Rydmark
M
M
Jansson
T
T
Gustavsson
T
T
D000465:Algorithms; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002415:Cats; D002467:Cell Nucleus / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000033:anatomy & histology* / Q000166:cytology; D003199:Computer Systems; D003626:Data Display; D003628:Database Management Systems; D003712:Dendrites / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008867:Microtomy; D009457:Neuroglia / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure; D012984:Software; D014584:User-Computer Interface