Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia coli K12 as a response to pollutant stress.
The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.
Faber
F
F
Department of Biology, TNO Institute of Environmental Sciences, Delft, The Netherlands.
Egli
T
T
Harder
W
W
D001221:Aspartate Carbamoyltransferase / Q000096:biosynthesis*; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D002104:Cadmium / Q000633:toxicity; D019256:Cadmium Chloride; D002712:Chlorides / Q000633:toxicity; D002733:Chlorophenols / Q000633:toxicity; D015180:Electrophoresis, Gel, Two-Dimensional; D004785:Environmental Pollutants / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010416:Pentachlorophenol / Q000633:toxicity
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002712:Chlorides; D002733:Chlorophenols; D004785:Environmental Pollutants; D002104:Cadmium; D010416:Pentachlorophenol; D001221:Aspartate Carbamoyltransferase; D019256:Cadmium Chloride