Characteristics and localization of a determinant conferring partial macrolide resistance in Staphylococcus aureus carrying plasmid.
Staphylococcus aureus TPR-27, a clinically isolated strain, showed constitutive resistance to some macrolide antibiotics (erythromycin, oleandomycin, spiramycin, and josamycin), but susceptibility to the other macrolide (tylosin, rokitamycin, and mycinamicin), lincosamide, and streptogramin type B antibiotics (PM-resistance). The PM-resistant strain TPR-27 has carried for visible plasmids. Attempts to eliminate the resistant determinant in terms of ethidium bromide (about 3 micrograms/ml) did not succeed, and every trial to transduce the PM-resistant determinant into rec- mutant ISP105 using phage 80L2 propagated on strain TPR-27 also failed at the frequency of less than 1.1 x 10(-10) transductants per plaque-forming unit. These results suggest that the PM-resistance determinant is localized in chromosomal DNA.
Matsuoka
M
M
Division of Microbiology, Hokkaido Institute of Pharmaceutical Sciences, Japan.
Endou
K
K
Yagi
K
K
Nakajima
Y
Y
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D018942:Macrolides; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010957:Plasmids; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D014161:Transduction, Genetic
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides