glh-1, a germ-line putative RNA helicase from Caenorhabditis, has four zinc fingers.
We have cloned a family of putative RNA helicases from the free-living nematode Caenorhabditis elegans. One of these, a cDNA that we call glh-1, most closely matches in sequence and expression the previously described germ-line helicases PL10 from mouse and vasa from Drosophila. The amino terminus of the predicted protein of glh-1 contains a set of glycine-rich repeats similar in location and sequence to those in the predicted vasa protein. However, unlike all other putative RNA helicases, glh-1 also contains four retroviral-type zinc fingers. The RNA expression pattern of this Caenorhabditis helicase correlates with the presence of germ-line tissue in the parasitic nematode Ascaris lumbricoides var. suum and with the presence of germ cells in wild type and several germ-line mutants of Caenorhabditis. In the germ-line mutants glp-4 and glp-1, additional larger species of glh-1 RNA exist, which correspond to different adenylylated forms of the glh-1 transcript; these may be specified by motifs in the 3' untranslated region of glh-1 that are similar to adenylylation control elements and nos response elements.
Roussell
D L
DL
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri-Columbia 65212.
Bennett
K L
KL
D000595:Amino Acid Sequence; D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D002874:Chromosome Mapping; D016384:Consensus Sequence; D017238:Genes, Helminth; D005854:Germ Cells / Q000502:physiology; D008969:Molecular Sequence Data; D011061:Poly A / Q000378:metabolism; D020365:RNA Helicases; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers
D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011061:Poly A; D012316:RNA Nucleotidyltransferases; D020365:RNA Helicases