Cloning and characterization of the Salmonella typhimurium-specific chemoreceptor Tcp for taxis to citrate and from phenol.
Salmonella typhimurium shows an attractant response to citrate and a repellent response to phenol, and a chemoreceptor mediating these responses has been identified and named Tcp (taxis to citrate and away from phenol). Tcp is one of the methyl-accepting chemotaxis proteins that have a molecular mass of approximately 60 kDa estimated by SDS/PAGE, and its methylation level is increased by citrate and decreased by phenol. Tcp also mediates an attractant response to metal-citrate complexes. The complete nucleotide sequence of the tcp coding region has been determined. The deduced amino acid sequence of Tcp, consisting of 547-amino acid residues, is homologous with that of the aspartate chemoreceptor of S. typhimurium. Thus, Tcp is another member of the bacterial transmembrane chemoreceptor family. Because citrate is a good carbon source for S. typhimurium but is not a carbon source for the closely related species Escherichia coli and because citrate utilization is used as a key diagnostic character to distinguish these species, it is reasonable to assume that Tcp is specific to S. typhimurium.
Yamamoto
K
K
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.
Imae
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002630:Chemotactic Factors / Q000235:genetics* / Q000378:metabolism; D002633:Chemotaxis; D002951:Citrates / Q000494:pharmacology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D019800:Phenol; D010636:Phenols / Q000494:pharmacology*; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
D002630:Chemotactic Factors; D002951:Citrates; D004269:DNA, Bacterial; D008565:Membrane Proteins; D010636:Phenols; C079113:Tcp receptor; D019800:Phenol