title: Growth and phenotypic characterization of Legionella species on semisolid media made with washed agar.
abstract: Legionella pneumophila (and 28 Legionella species) grew efficiently on charcoal-free, buffered yeast extract medium made with washed agar and without apparent loss of infectivity for U937 cells. Because charcoal-free, buffered yeast extract is transparent, it is a suitable base for indicator media and pigment detection. In standard media, charcoal apparently prevents agar contaminants from inhibiting Legionella growth.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan, Ann Arbor 48109-0620.
authors.forename: J E
authors.initials: JE
authors.lastname: Rogers
authors.forename: G W
authors.initials: GW
authors.lastname: Jones
authors.forename: N C
authors.initials: NC
authors.lastname: Engleberg
mesh_terms: D000362:Agar / Q000737:chemistry; D002606:Charcoal; D015169:Colony Count, Microbial; D006801:Humans; D007875:Legionella / Q000254:growth & development*; D015003:Yeasts
chemical_list: D002606:Charcoal; D000362:Agar