title: Immunological identification of the major disulfide-linked light component of silk fibroin.
abstract: The light protein components of silk fibroin were analyzed by Western blotting using polyclonal antibodies against two synthetic peptides that are segment of the light (L-) chain [Yamaguchi et al. (1989) J. Mol. Biol. 210, 127-139] and P25 [Chevillard et al. (1986) Sericologia 26, 435-449], respectively, and against the whole L-chain. Both the L-chain and P25 were present with fibroin in the lumen of the posterior silk gland and in the cocoon. The L-chain was identified as the major light component that was disulfide-linked to the fibroin heavy (H-) chain, whereas P25 was a minor component whose association with the H-chain was suggested to be non-covalent.
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: S
authors.initials: S
authors.lastname: Mizuno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D012831:Bombyx / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005349:Fibroins / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data
chemical_list: D004220:Disulfides; D005349:Fibroins