[Dynamics in changes of a1-thymosin level under irradiation of mice thymus in vivo and in vitro: relation to the antibody-antigen production of epithelial thymus reticulum].
In 3- and 9-month experiments with mice, a study was made of the effect of radiation on serum alpha 1-thymosine concentration after whole-body irradiation and local exposure of the thymus at doses of 1-20 Gy. The effect of 137Cs-gamma-rays on the in vitro cultured thymus stroma cells, with respect to alpha 1-thymosine secretion, and the influence of local irradiation of the thymus of production of autoantibodies that react with epithelial thymus cells were studied. Both whole-body irradiation and local exposure of the thymus were shown to cause changes in the alpha 1-thymosine content of the blood plasma. The direction and dynamics of the changes observed are different with whole-body and local exposure. Irradiation of cultured thymus cells of mice causes alterations in alpha 1-thymosine secretion, that is, stimulation at a dose of 1 Gy and inhibition at higher doses. With respect to dose- and time-response, these changes are closer to those observed in alpha 1-thymosine concentration in mouse serum after whole-body irradiation than after local exposure of the thymus in vivo. At remote times after local irradiation of the thymus with doses of 1-10 Gy, autoantibodies are found in mouse serum that react with epithelial cells of the thymus stroma. Autoantibodies are absent at doses of 15 and 20 Gy.
Kuzmenok
O I
OI
Beliakov
I M
IM
Iarilin
A A
AA
Arshinov
V Iu
VIu
D000818:Animals; D001323:Autoantibodies / Q000528:radiation effects*; D001324:Autoantigens / Q000528:radiation effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004848:Epithelium / Q000276:immunology / Q000528:radiation effects; D005260:Female; D005500:Follow-Up Studies; D017619:Hemibody Irradiation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000097:blood / Q000528:radiation effects; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000528:radiation effects*; D013997:Time Factors; D014916:Whole-Body Irradiation
D001323:Autoantibodies; D001324:Autoantigens; D013947:Thymosin; D000077596:Thymalfasin