Activated synovial T cell clones from a patient with rheumatoid arthritis induce proliferation of autologous peripheral blood-derived T cells.
In order to investigate cellular interactions involved in the development of human autoimmune disease, a synovial fluid-derived T cell clone reactive with mycobacterial antigens, termed k38, was employed as a stimulus for autologous peripheral blood mononuclear cells (PBMC). Stimulator cells were used either activated with immobilized OKT3 mAb or in a resting state. Activated k38 cells triggered PBMC to proliferate. A T cell line prepared by coculturing autologous PBMC with irradiated activated k38 cells proliferated upon stimulation with activated k38 cells in the presence of PBMC as a source of accessory cells, as did T cell clones that were subsequently isolated from this line. Blocking studies revealed that proliferation of the anti-k38 line and anti-k38 clones in response to stimulation with clone k38 could be inhibited by monoclonal antibodies against a variety of cellular determinants including HLA class I and LFA-1 beta. It was demonstrated that the antigen reactivity of clone k38 was modulated by the presence of anti-k38 clones. These data provide a model for understanding the cellular interactions that may take place in vivo in the evolution of the chronic synovial inflammatory process.
van Laar
J M
JM
Department of Rheumatology, University Hospital, Leiden, The Netherlands.
Miltenburg
A M
AM
Verdonk
M J
MJ
Leow
A
A
Elferink
B G
BG
Daha
M R
MR
de Vries
R R
RR
Breedveld
F C
FC
D000911:Antibodies, Monoclonal; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology*; D002455:Cell Division; D002460:Cell Line / Q000276:immunology; D002999:Clone Cells / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D016853:Muromonab-CD3 / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D016853:Muromonab-CD3