Effects of endothelin-1 on postjunctionally-mediated purinergic and adrenergic components of rat vas deferens contractile responses.
The effects of endothelin-1 (ET-1) on electrically- or drug-induced contractile responses mediated by purinergic or adrenergic receptors were studied in isolated prostatic portion of rat vas deferens. ET-1 (0.01 nM to 30 nM) concentration-dependently increased the contractions evoked by electrical field stimulation (EFS, 0.3 msec, 30 V, 8 Hz, 300 pulses). In the presence of prazosin, ET-1 (3 nM) strongly enhanced the prazosin-resistant responses to EFS, while after desensitization of purinergic receptors induced by alpha,beta-methylene adenosine 5'-triphosphate (mATP) the peptide only tended to enhance the mATP-resistant component of the electrically-evoked contractions. ET-1 failed to change the nonpurinergic nonadrenergic responses to electrical stimulation revealing after simultaneous administration of prazosin and mATP. ET-1 concentration-dependently increased the contractile effects of exogenous ATP (30 microM). The effect of ET-1 (3 nM) was not changed after tetrodotoxin (TTX, 0.3 microM) and guanethidine (10 microM). In the presence of TTX and guanethidine ET-1 potentiated the contractile effects of low (0.01-1 microM) concentrations of noradrenaline (NA) and did not change the contractions induced by NA at concentrations higher than 3 microM. Therefore, ET-1 exerted a potentiating effect on the contractility of the prostatic portion of rat vas deferens via postjunctional mechanisms underlying mainly the purinergic and partly the adrenergic smooth muscle contractile responses.
Mutafova-Yambolieva
V
V
Department of Experimental Pharmacology, Bulgarian Academy of Sciences, Sofia.
Radomirov
R
R
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D016232:Endothelins / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011983:Receptors, Purinergic / Q000187:drug effects*; D013564:Sympathetic Nervous System / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology; D014649:Vas Deferens / Q000187:drug effects*
D016232:Endothelins; D011983:Receptors, Purinergic; D013779:Tetrodotoxin; D000255:Adenosine Triphosphate; D009638:Norepinephrine