title: Thiamine-repressible expression vectors pREP and pRIP for fission yeast.
abstract: The promoter and polyadenylation signal of the thiamine-repressible gene nmt1 of Schizosaccharomyces pombe have been used to construct the pREP extrachromosomally replicating plasmids and the pRIP integrative expression plasmids. These plasmids permit thiamine-mediated control of transcription to be applied to cloned genes.
authors.affiliation: Sclavo Research Centre, Siena, Italy.
authors.forename: K
authors.initials: K
authors.lastname: Maundrell
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D013831:Thiamine / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D013831:Thiamine