Expression of amyloid precursor protein in a neuronal cell line: functional activity of proximal regulatory elements.
To study cell type-specific regulation of the gene for amyloid precursor protein (APP), we have analysed the human APP promoter for transcriptional activity and interaction with nuclear proteins in the neuronal cell line NG108-15. Sequences from -203 to +104 were sufficient for promoter function and regulatory elements within this region (-128 to -63) were identified by DNase I protection experiments. These results suggest that essentially the same proximal elements are recognized by nuclear factors from both neuronal and nonneuronal cells.
Bourbonnière
M
M
Department of Neurology and Neurosurgery, McGill University, Montreal, Que., Canada.
Nalbantoglu
J
J
D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis*; D001483:Base Sequence; D002460:Cell Line; D005809:Genes, Regulator; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D016564:Amyloid beta-Protein Precursor