title: Infection of macrophages with lymphotropic human immunodeficiency virus type 1 can be arrested after viral DNA synthesis.
abstract: Lymphotropic strains of human immunodeficiency virus type 1 (HIV-1), including HTLV-IIIB, replicate poorly in macrophages. We have shown previously that lymphotropic HIV-1 fuses equally well with T lymphocytes and macrophages (M. J. Potash, M. Zeira, Z.-B. Huang, T. Pearce, E. Eden, H. Gendelman, and D. J. Volsky, Virology 188:864-868, 1992), suggesting that events in the virus life cycle following virus-cell fusion limit virus replication. We report here that HIV-1 DNA is synthesized efficiently in either ADA or HTLV-IIIB infected alveolar macrophages or monocyte-derived macrophages within 24 h of virus infection, as observed by polymerase chain reaction for amplification of viral DNA sequences from the gag gene. Infection by a cloned lymphotropic HIV-1 strain, N1T-A, also leads to viral DNA synthesis. However, circular viral DNA was detected during strain ADA infection but not during HTLV-IIIB or N1T-A infection of monocyte-derived macrophages. These findings indicate that during replication of lymphotropic HIV-1 in macrophages, all steps of the virus life cycle up to and including reverse transcription take place and that defects in later events, including DNA migration to the nucleus, may account for the limited production of viral proteins.
authors.affiliation: Division of Pulmonary Medicine, St. Lukes/Roosevelt Hospital Center, College of Physicians and Surgeons, Columbia University, New York, New York 10019.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Huang
authors.forename: M J
authors.initials: MJ
authors.lastname: Potash
authors.forename: M
authors.initials: M
authors.lastname: Simm
authors.forename: M
authors.initials: M
authors.lastname: Shahabuddin
authors.forename: W
authors.initials: W
authors.lastname: Chao
authors.forename: H E
authors.initials: HE
authors.lastname: Gendelman
authors.forename: E
authors.initials: E
authors.lastname: Eden
authors.forename: D J
authors.initials: DJ
authors.lastname: Volsky
mesh_terms: D004279:DNA, Viral / Q000096:biosynthesis; D016655:HIV Core Protein p24 / Q000378:metabolism; D015497:HIV-1 / Q000254:growth & development*; D008214:Lymphocytes / Q000382:microbiology*; D008264:Macrophages / Q000382:microbiology*; D013997:Time Factors; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D016655:HIV Core Protein p24