Autoimmune Addison's disease. Analysis of autoantibody binding sites on human steroid 21-hydroxylase.
Human steroid 21-hydroxylase (21-OH) expressed in an in vitro translation system was found to react specifically with adrenal autoantibodies from patients with Addison's disease. The epitopes on 21-OH which reacted with autoantibodies were studied by incorporating a series of terminal and internal deletions into the 21-OH gene and analysing the expressed proteins by Western blotting. N-Terminal deletions up to amino acid 280 had no effect on autoantibody binding whereas a series of C-terminal deletions and truncations (amino acids 281-494) showed marked effects. Our results indicate that a central segment (281-379) and a C-terminal segment (380-494) of 21-OH interact to form at least one major autoantibody binding site.
Wedlock
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N
Endocrine Immunology Unit, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Asawa
T
T
Baumann-Antczak
A
A
Smith
B R
BR
Furmaniak
J
J
D000224:Addison Disease / Q000276:immunology*; D000328:Adult; D001323:Autoantibodies / Q000276:immunology*; D001483:Base Sequence; D001666:Binding Sites, Antibody; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D015183:Restriction Mapping; D017384:Sequence Deletion; D013255:Steroid 21-Hydroxylase / Q000235:genetics / Q000276:immunology*
D001323:Autoantibodies; D009838:Oligodeoxyribonucleotides; D013255:Steroid 21-Hydroxylase