The combined assessment of cellular apoptosis, mitochondrial function and proliferative response to pokeweed mitogen has prognostic value in SIV infection.
Using the assessment of the mitochondrial metabolic activity of freshly isolated blood mononuclear cells, the flow cytometric detection of apoptosis and of the proliferative responses to PWM, SIV-infected macaques were classified in: stage 0, which included all animals with unaffected parameters, and stages 1, 2, and 3, which included animals having one, two, or all three parameters affected, respectively. This novel three-parametric staging system (ISS) provides a new prognostic tool in the longitudinal study of SIV infection.
del Llano
A M
AM
Department of Microbiology and Medical Zoology, University of Puerto Rico, San Juan 00936-5067.
Amieiro-Puig
J P
JP
Kraiselburd
E N
EN
Kessler
M J
MJ
Málaga
C A
CA
Lavergne
J A
JA
D000818:Animals; D017209:Apoptosis; D015658:HIV Infections / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D008928:Mitochondria / Q000378:metabolism*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011379:Prognosis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
D011043:Pokeweed Mitogens