Lack of glucocorticoids sustains the stress-induced release of noradrenaline in the anterior hypothalamus.
The release of endogenous noradrenaline in the anterior hypothalamus was studied with microdialysis perfusion in freely moving rats that were subjected to immobilization stress. Experiments were carried out in sham-adrenalectomized and adrenalectomized rats that were first given drinking water containing corticosterone for 5 days following surgery and then switched to a corticosterone-free diet the day before stress application. One group of these adrenalectomized animals was injected with dexamethasone. Basal release of noradrenaline collected in 20-min fractions was similar in the three groups of animals and averaged 24 fmol. The recovery of the probe was about 10%. In sham-adrenalectomized rats application of 20-min immobilization stress increased noradrenaline release to 310% of baseline in the sample collected during stress application. A significant increase (+ 175% of baseline) was still observed in the next 20-min sample. Subsequent values were all identical to baseline. In adrenalectomized rats lacking exogenous corticosterone the stress-induced release of noradrenaline was prolonged with noradrenaline levels remaining elevated for 2 h after the onset of stress. The total noradrenaline release during this entire period was about 2.5 times higher in adrenalectomized than in sham-operated rats. However, the maximal increase during the period of immobilization was not significantly affected. Treatment with dexamethasone prevented the prolonged increase in noradrenaline release but did not affect the increase during the period of stress. While glucocorticoids do not appear to affect the increased release of NA in the anterior hypothalamus during the period of stress, they act to limit the duration of this activation after the application of stress.(ABSTRACT TRUNCATED AT 250 WORDS)
Vetrugno
G C
GC
Instituto di Ricerche Farmacologiche Mario Negri, Milano, Italia.
Lachuer
J
J
Perego
C
C
Miranda
E
E
De Simoni
M G
MG
Tappaz
M
M
D000315:Adrenalectomy; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000494:pharmacology; D003956:Dialysis; D005110:Extracellular Space / Q000378:metabolism; D005938:Glucocorticoids / Q000172:deficiency*; D007032:Hypothalamus, Anterior / Q000378:metabolism*; D007103:Immobilization; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000209:etiology / Q000378:metabolism*
D005938:Glucocorticoids; D003907:Dexamethasone; D009638:Norepinephrine