Crystallization and preliminary crystallographic investigations of the soluble glucose dehydrogenase from Acinetobacter calcoaceticus.
Single crystals of the soluble glucose dehydrogenase (GDH) from Acinetobacter calcoaceticus have been grown by the vapour diffusion method. These crystals diffract to beyond 2.1 A and are suitable for X-ray crystallography. The space group was determined to be P2(1) with unit cell parameters a = 55.5 A, b = 104.5 A, c = 86.5 A and beta = 99.8 degrees. One asymmetric unit contains a dimer of the GDH molecule.
Schlunegger
M P
MP
Department of Biotechnology, Ciba, Basel, Switzerland.
Grütter
M G
MG
Streiff
M B
MB
Olsthoorn
A J
AJ
Duine
J A
JA
D016954:Acinetobacter calcoaceticus / Q000737:chemistry / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000737:chemistry*; D012995:Solubility
D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase