Leukocyte common antigen-related phosphatase (LRP) gene structure: conservation of the genomic organization of transmembrane protein tyrosine phosphatases.
The leukocyte common antigen-related protein tyrosine phosphatase (LRP) is a widely expressed transmembrane glycoprotein thought to be involved in cell growth and differentiation. Similar to most other transmembrane protein tyrosine phosphatases, LRP contains two tandem cytoplasmic phosphatase domains. To understand further the regulation and evolution of LRP, we have isolated and characterized mouse lambda genomic clones. Thirteen genomic clones could be divided into two non-overlapping clusters. The first cluster contained the transcription initiation site and the exon encoding most of the 5' untranslated region. The second cluster contained the remaining exons encoding the protein and the 3' untranslated region. The gene consists of 22 exons spanning over 75 kb. The distance between exon 1 and exon 2 is at least 25 kb. Characterization of the 5' ends of LRP mRNA by S1 nuclease protection identifies putative initiation start sites within a G/C-rich region. The upstream region does not contain a TATA box. Comparison of the LRP gene structure to the mammalian protein tyrosine phosphatase gene, CD45, shows striking similarities in size and genomic organization.
Wong
E C
EC
Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110.
Mullersman
J E
JE
Thomas
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007438:Introns; D017493:Leukocyte Common Antigens / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4; D011956:Receptors, Cell Surface
D015342:DNA Probes; D018076:DNA, Complementary; D006023:Glycoproteins; D011956:Receptors, Cell Surface; D010749:Phosphoprotein Phosphatases; D017493:Leukocyte Common Antigens; C516841:PTPRA protein, human; D017027:Protein Tyrosine Phosphatases; C516843:Ptpra protein, mouse; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4