title: Hybrid sterility-6: a mouse t complex locus controlling sperm flagellar assembly and movement.
abstract: Mouse t haplotypes alter sperm differentiation, resulting in abnormal sperm movement and sterility. In previous studies, a locus responsible for hybrid sterility in the genus Mus,Hst-4, was mapped to the distal inversion of the t complex on chromosome 17. Here we report the identification and characterization of two additional hybrid sterility loci, Hst-5 and Hst-6, that map to the same inversion. We further show that an abnormality in sperm flagellar curvature deriving from interactions between t haplotypes and the M. spretus allele of Hst-6 is indistinguishable from one exhibited by sperm from mice carrying two t haplotypes. Additionally, we demonstrate that this latter phenotype maps to the distal inversion of t haplotypes. Morphological and functional studies of Hst-6 mutant sperm also imply that the product(s) of Hst-6 is a spermatogenic-specific protein, important for assembly and function of the sperm axoneme. Thus, Hst-6 provides direct access to the molecular basis of t haplotype-specific alterations in sperm function that emanate from the t complex distal inversion.
authors.affiliation: Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
authors.forename: S H
authors.initials: SH
authors.lastname: Pilder
authors.forename: P
authors.initials: P
authors.lastname: Olds-Clarke
authors.forename: D M
authors.initials: DM
authors.lastname: Phillips
authors.forename: L M
authors.initials: LM
authors.lastname: Silver
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D006239:Haplotypes; D006720:Homozygote; D007248:Infertility, Male / Q000235:genetics* / Q000662:veterinary; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins / Q000235:genetics*; D013081:Sperm Motility; D013082:Sperm Tail / Q000502:physiology*; D013735:Testicular Hormones / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D013735:Testicular Hormones; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases