Nucleotide sequence and style-specific expression of a novel proline-rich protein gene from Nicotiana alata.
cDNA clones encoding a novel proline-rich protein (NaPRP4) have been isolated from a Nicotiana alata stylar cDNA library. The N-terminal part of the derived protein is highly rich in proline (32.2%) and contains several repeats such as Lys-Pro-Pro (7 times) and Pro-Thr-Lys-Pro-Pro-Thr-Tyr-Ser-Pro-Ser-Lys-Pro-Pro (twice); the C-terminal part, on the other hand, has a lower proline content (9.9%) and contains two potential N-glycosylation sites and all the six cysteine residues. Northern blot and in situ hybridisation analyses indicate that expression of the NaPRP4 gene is restricted to cells of the transmitting tract of the style.
Chen
C G
CG
Plant Cell Biology Research Centre, University of Melbourne, Parkville, Victoria, Australia.
Mau
S L
SL
Clarke
A E
AE
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D007887:Fabaceae / Q000235:genetics; D015723:Gene Library; D006023:Glycoproteins; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development
D006023:Glycoproteins; C079482:NaPRP4 protein, Nicotiana alata; D010940:Plant Proteins; D004247:DNA