Effect of age on the ability of rat liver tissues to transform chemical promutagens to mutagens.
Age-related changes in drug metabolism of the liver from male Lou rats were determined by measuring changes in the production of mutagens. Activation of aflatoxin B1 (AFB1), 2-aminoanthracene (2AA) and benzo[a]pyrene (B[a]P) to mutagenic derivatives was assayed using the Ames salmonella test system. The promutagens were incubated with tissue fractions isolated from rats ranging in age from 3 weeks to 18 months. Hepatic activation of AFB1 and 2AA changes with age and is maximal from 4 to 10 months. In contrast, no significant modification with age was observed for the activation of B[a]P. It is concluded that the composition of different cytochrome P-450 fractions is modified with age thereby altering the specific ability to convert different promutagens to mutagens.
Hilali
A
A
Laboratory of Teratogenesis and Mutagenesis, University of Louvain, Brussels, Belgium.
Crutzen-Fayt
M C
MC
Gerber
G B
GB
Léonard
A
A
D016604:Aflatoxin B1 / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000873:Anthracenes / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000378:metabolism; D001711:Biotransformation; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D051381:Rats
D000873:Anthracenes; D009153:Mutagens; D001564:Benzo(a)pyrene; C006593:2-anthramine; D016604:Aflatoxin B1