Selection of acrosome-reacted human spermatozoa and their fusing ability by micro-injection into the perivitelline space of hamster eggs.
Previously we reported the selective binding of human acrosome-reacted spermatozoa to monoclonal antibody-coated paramagnetic beads. In the present paper, the fusing ability of spermatozoa that bound to the beads was estimated by micro-injecting them into the perivitelline space of hamster oocytes. Acrosome-reacted human spermatozoa were collected by monoclonal antibody-coated paramagnetic beads. Bead-bound spermatozoa were selected under a microscope and micro-injected into the perivitelline space of hamster oocytes. When the injection was begun 5 min after mixing spermatozoa with the beads, 6.6% of the injected spermatozoa fused with an oocyte. However, when the injection was begun 30 min after the mixing, no fusion occurred. Moreover, when spermatozoa were injected with the beads attached to their head, no fusion was observed. The fusing ability of spermatozoa thus prepared was compared with that of randomly chosen and injected spermatozoa. Using spermatozoa from 10 healthy volunteers, 22 independent experiments were performed. The overall fusion rate of injected, randomly selected spermatozoa was 2.8%, whilst that of bead-selected spermatozoa was 7.3%. Selection of spermatozoa by beads appeared to be beneficial to the fusion event.
Ying
X
X
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Okabe
M
M
Mimura
T
T
D000177:Acrosome; D000818:Animals; D000911:Antibodies, Monoclonal; D002459:Cell Fusion / Q000502:physiology; D006224:Cricetinae; D005260:Female; D006801:Humans; D008280:Magnetics; D008297:Male; D008845:Microinjections; D008846:Micromanipulation; D008863:Microspheres; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D014817:Vitelline Membrane
D000911:Antibodies, Monoclonal