title: Kinetics of expression of two major Plasmodium berghei antigens in the mosquito vector, Anopheles stephensi.
abstract: Expression of a 21 kDa determinant (Pbs21), first detected on the surface of ookinetes, and of the circumsporozoite protein (CSP) was studied by immunofluorescence and Western blots during the developmental cycle of Plasmodium berghei in the mosquito Anopheles stephensi. The expression of Pbs21 was predominantly localised on the ookinete surface one day after the infectious blood meal, and thereafter reactivity declined to a minimum on days 2 and 3, the time of onset of oocyst development. A gradual increase in fluorescence was observed on the oocysts from day 6 that was retained until day 17 post-infection. In contrast, sporozoites released from oocysts or salivary glands showed little or no antibody labelling with anti-Pbs21. Circumsporozoite protein was not detectable in any midgut preparations until 5-6 days after feeding, when reactivity was observed against immature oocysts. Expression then continued and increased throughout oocyst and sporozoite development. Western blots confirmed that Pbs21 was expressed minimally during the oocyst development but was not detectable in sporozoites. Co-localisation of anti-Pbs21 and anti-CSP monoclonal antibodies to the 50 kDa and 60 kDa bands in Western blots of sporozoite suggests immunological cross-reactivity between the CSP and the anti-21 kDa antibodies.
authors.affiliation: Department of Biology, Imperial College of Science, Technology and Medicine, London, United Kingdom.
authors.forename: A B
authors.initials: AB
authors.lastname: Simonetti
authors.forename: P F
authors.initials: PF
authors.lastname: Billingsley
authors.forename: L A
authors.initials: LA
authors.lastname: Winger
authors.forename: R E
authors.initials: RE
authors.lastname: Sinden
mesh_terms: D000818:Animals; D000852:Anopheles / Q000276:immunology / Q000469:parasitology*; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003429:Cross Reactions; D007303:Insect Vectors / Q000276:immunology / Q000469:parasitology*; D007700:Kinetics; D051379:Mice; D008856:Microscopy, Fluorescence; D010962:Plasmodium berghei / Q000276:immunology*; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan; C064783:sexual stage specific protein, Plasmodium