title: Expression of a common cellular phospholipase A2 by human intrauterine tissues.
abstract: A subclone of the three prime (3') end of a human extrapancreatic phospholipase cDNA has been generated using the polymerase chain reaction. This has been used to detect mRNA transcripts in RNA extracted from human amnion, chorion-decidua, placenta and myometrium. The carboxyl-terminus of phospholipases A2 is poorly conserved but hybridisation remained stable under conditions of high stringency. This supports the hypothesis that these tissues express a common cellular phospholipase A2 which is identical to that expressed in platelets.
authors.affiliation: Action Research Laboratory for the Molecular Biology of Fetal Development, Royal Post Graduate Medical School, Queen Charlotte's and Chelsea Hospital, London, England.
authors.forename: P
authors.initials: P
authors.lastname: Bennett
authors.forename: D
authors.initials: D
authors.lastname: Slater
authors.forename: P
authors.initials: P
authors.lastname: Stanier
authors.forename: G
authors.initials: G
authors.lastname: Moore
mesh_terms: D000650:Amnion / Q000201:enzymology; D001483:Base Sequence; D002823:Chorion / Q000201:enzymology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D003656:Decidua / Q000201:enzymology; D005091:Exons; D005260:Female; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009215:Myometrium / Q000201:enzymology; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D014599:Uterus / Q000201:enzymology*
chemical_list: D012333:RNA, Messenger; D004247:DNA; D010741:Phospholipases A; D054467:Phospholipases A2