Prospects for estimating nucleotide divergence with RAPDs.
The technique of random amplification of polymorphic DNA (RAPD), which is simply polymerase chain reaction (PCR) amplification of genomic DNA by a single short oligonucleotide primer, produces complex patterns of anonymous polymorphic DNA fragments. The information provided by these banding patterns has proved to be of great utility for mapping and for verification of identity of bacterial strains. Here we consider whether the degree of similarity of the banding patterns can be used to estimate nucleotide diversity and nucleotide divergence. With haploid data, fragments generated by RAPD-PCR can be treated in a fashion very similar to that for restriction-fragment data. Amplification of diploid samples, on the other hand, requires consideration of the fact that presence of a band is dominant to absence of the band. After describing a method for estimating nucleotide divergence on the basis of diploid samples, we summarize the restrictions and criteria that must be met when RAPD data are used for estimating population genetic parameters.
Clark
A G
AG
Department of Biology, Pennsylvania State University, University Park 16802.
Lanigan
C M
CM
D003198:Computer Simulation; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D004171:Diploidy; D014644:Genetic Variation; D005828:Genetics, Population; D006238:Haploidy; D008433:Mathematics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004247:DNA