Physical map of the extremely thermophilic bacterium Thermus thermophilus HB27 chromosome.
The physical map of the chromosome of Thermus thermophilus HB27 was constructed using three restriction enzymes; EcoRI, SspI and MunI by applying pulsed-field gel electrophoresis techniques. Although the genome size of 1.82 Mb was almost the same as that (1.74 Mb) reported for T. thermophilus HB8 [Borges, K.M., and P.L. Bergquist. (1993) J. Bacteriol. 175, 103-110], the MunI cleavage maps were different. A 240 kb plasmid was detected in HB27, and its physical map was also constructed. In addition, several genes were located on the chromosomal physical map.
Tabata
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K
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Kosuge
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T
Nakahara
T
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Hoshino
T
T
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D010957:Plasmids; D016963:Thermus thermophilus / Q000235:genetics*
D004262:DNA Restriction Enzymes