[Detection of antibody to varicella zoster virus by immune adherence hemagglutination].
Anti varicella-zoster virus (VZV) antibodies were detected by an immune adherence hemagglutination (IAHA) test, and were compared with the CF test, IFA test and ELISA test, respectively. Type O, Rh-positive RBC for IAHA was obtained from five healthy volunteers. All five RBCs had sufficient sensitivity as the indicator cell. Optimum incubation temperature was 37 degrees C in the serum and in the complement. The complement was obtained from guinea pig sera, and the most suitable concentration was 1;100. The convalescent VZV antibody titers were similar to the values obtained from any of the methods previously mentioned. However, mean titers measured by CF were about two-to fourfold lower than in the values of IAHA. Seroconversion rates of the live VZV vaccine, as detected by CF and IFA were relatively low (CF; 76%, IFA; 56%). In contrast, those obtained from ELISA and IAHA showed perfectly (100%). These results indicate that IAHA has sufficient sensitivity and specificity in the detection of VZV antibodies. In addition, the IAHA test is thought to be a rapid and easy test to perform in ordinary laboratories.
Nakajima
N
N
Department of Pediatrics, St. Marianna University School of Medicine.
Minohara
Y
Y
Goshima
F
F
Arimoto
Y
Y
Kaku
H
H
Goshima
T
T
Katoh
T
T
Fukazawa
T
T
Asano
Y
Y
Yamauchi
T
T
D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D006386:Hemagglutination Tests / Q000379:methods; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant
D000914:Antibodies, Viral