In vivo effects of recombinant interleukin-11 on myelopoiesis in mice.
Purified recombinant human interleukin-11 (rhuIL-11) was assessed for its in vivo effects on the proliferation and differentiation of hematopoietic progenitors as well as its capacity to accelerate the recovery of a drug-suppressed hematopoietic system. Dosage and time sequence studies demonstrated that administration of IL-11 to normal mice resulted in increases in absolute numbers of femoral marrow and splenic myeloid (granulocyte-macrophage colony-forming unit [CFU-GM], burst-forming unit-erythroid [BFU-E], CFU-granulocyte, erythroid, macrophage, megakaryocyte) progenitor cells and in stimulation of these progenitors to a higher cell cycling rate. This was associated with increased numbers of circulating neutrophils. Administration of IL-11 to mice pretreated with cyclophosphamide decreased the time required to regain normal levels of neutrophil and platelet counts in peripheral blood. In addition, IL-11 accelerated reconstitution to normal range of myeloid progenitors from bone marrow and spleen of myelosuppressed mice. These data suggest that IL-11 may play an important role in the regulation of hematopoiesis, and the application of this novel cytokine may have clinical therapeutic benefits.
Hangoc
G
G
Department of Medicine (Hematology/Oncology), Indiana University School of Medicine, Indianapolis.
Yin
T
T
Cooper
S
S
Schendel
P
P
Yang
Y C
YC
Broxmeyer
H E
HE
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D004906:Erythrocyte Count; D015672:Erythroid Precursor Cells / Q000166:cytology; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D017370:Interleukin-11 / Q000494:pharmacology*; D007958:Leukocyte Count; D008264:Macrophages / Q000166:cytology; D008533:Megakaryocytes / Q000166:cytology; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D017370:Interleukin-11; D011994:Recombinant Proteins