title: Suppression of Schistosoma bovis egg production in cattle by vaccination with either glutathione S-transferase or keyhole limpet haemocyanin.
abstract: Two of the antigens which have shown vaccine potential in animal experiments against Schistosoma mansoni are glutathione-S-transferase (GST) and GP38, protective epitopes of which are shared with keyhole limpet haemocyanin (KLH). We therefore tested S. bovis GST and KLH for vaccine efficacy against S. bovis in the natural Zebu cattle host. In a preliminary experiment three vaccinations with a total of 1.39 mg of native GSTs of S. bovis induced specific antibody at the time of challenge as detected by Western blotting and ELISA and mean faecal egg counts between weeks 6-10 post-challenge were reduced by 56.4 to 82.5% compared to non-vaccinated controls. Mean adult worm recoveries and tissue egg densities in large intestine and liver samples were also reduced in the vaccinated group, but these differences were not statistically significant. In a subsequent experiment one group of calves was vaccinated with a similar schedule to that used above; a second group of calves was given only two injections of GST (total 0.48 mg protein); a third group of calves was vaccinated twice with a total of 2.0 mg KLH in PBS. All three vaccination schedules induced specific antibody. Both GST vaccination schedules induced significant reductions in faecal egg counts compared to non-vaccinated controls and in this experiment tissue egg densities were also significantly reduced. A striking finding, however, was that adult worm counts were not reduced by vaccination. An essentially similar outcome resulted from KLH vaccination, since there were significant reductions in faecal and tissue egg counts in the absence of a reduction in adult worm numbers.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, University of Khartoum, Sudan.
authors.forename: H O
authors.initials: HO
authors.lastname: Bushara
authors.forename: M E
authors.initials: ME
authors.lastname: Bashir
authors.forename: K H
authors.initials: KH
authors.lastname: Malik
authors.forename: M M
authors.initials: MM
authors.lastname: Mukhtar
authors.forename: F
authors.initials: F
authors.lastname: Trottein
authors.forename: A
authors.initials: A
authors.lastname: Capron
authors.forename: M G
authors.initials: MG
authors.lastname: Taylor
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005982:Glutathione Transferase / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology*; D010270:Parasite Egg Count; D012547:Schistosoma / Q000187:drug effects / Q000276:immunology*; D012552:Schistosomiasis / Q000517:prevention & control / Q000662:veterinary*; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D006433:Hemocyanins; D005982:Glutathione Transferase; C032808:keyhole-limpet hemocyanin