title: Subzonal multiple sperm injection in the treatment of previous failed human in vitro fertilization.
abstract: OBJECTIVE
  To assess the value of subzonal sperm injection in previous failed spontaneous in vitro fertilization (IVF).
  
  
  METHODS
  Prospective study on patients with at least one cycle of previous failed spontaneous IVF.
  
  
  METHODS
  Human Reproductive Biology Unit, Soliman Fakeeh Hospital, Jeddah, Saudi Arabia.
  
  
  METHODS
  Thirty-nine patients with prolonged infertility with at least one previous cycle of failure of spontaneous fertilization of any oocyte.
  
  
  RESULTS
  Seventy-eight of 276 oocytes successfully injected with three to four sperms were fertilized. Fertilized rate was 28.2%. Polyspermy was observed in 6 (8%) of the fertilized oocytes. Sixty-six (92%) of the 72 with monospermic fertilization cleaved normally and were replaced in the uterine cavity. Pregnancy occurred in 6 (23%) of the women who had embryos replaced.
  
  
  CONCLUSIONS
  Multiple sperm injection into the oocyte subzonal space should be considered for the treatment of patients with previous failed spontaneous IVF.
authors.affiliation: Human Reproductive Biology Unit, Soliman Fakeeh Hospital, Jeddah, Saudi Arabia.
authors.forename: D A
authors.initials: DA
authors.lastname: Imoedemhe
authors.forename: A B
authors.initials: AB
authors.lastname: Sigue
mesh_terms: D000328:Adult; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D005500:Follow-Up Studies; D006801:Humans; D007267:Injections; D007317:Insemination, Artificial, Homologous / Q000379:methods*; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D011256:Pregnancy Outcome; D013094:Spermatozoa; D017211:Treatment Failure