[The value of hybridization-in-situ technique in the diagnosis of condyloma acuminatum].
By using 35S-dCTP labelled HPV16 probe, the HPV DNA sequence in 32 cases of condyloma acuminatum and suspicious condyloma acuminatum of vulva and vagina as well as 18 cases of papillomas at different anatomical sites were detected. The results showed that the HPV DNA sequences were positive in all 25 cases of condyloma acuminata which were typical both clinically and pathologically and in 6/7 of the suspicious cases; whereas in only 1/18 of the papillomas at various sites were they positive. Thus, nucleic acid hybridization-in-situ technique combined with histopathology seemed to be of great value for the diagnosis and differential diagnosis of condyloma acuminatum.
Li
D C
DC
Department of Pathology PUMC Hospital, CAMS, Beijing.
D003218:Condylomata Acuminata / Q000175:diagnosis* / Q000382:microbiology; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000382:microbiology; D010212:Papilloma / Q000175:diagnosis / Q000382:microbiology; D027383:Papillomaviridae / Q000302:isolation & purification; D014625:Vaginal Neoplasms / Q000175:diagnosis* / Q000382:microbiology; D014846:Vulvar Neoplasms / Q000175:diagnosis* / Q000382:microbiology
D015344:DNA Probes, HPV; D004279:DNA, Viral