Absence of a role for DNA polymerase II in SOS-induced translesion bypass of phi X174.
In order to examine the possible role of Escherichia coli DNA polymerase II in SOS-induced translesion bypass, Weigle reactivation and mutation induction were measured with single-stranded phi X174 transfecting DNA containing individual lesions. No decrease in bypass of thymine glycol or cyclobutane pyrimidine dimers in the absence of DNA polymerase II was observed. Furthermore, DNA polymerase II did not affect bypass of abasic sites when either survival or mutagenesis was the endpoint. Lastly, repair of gapped DNA molecules, intermediates in methyl-directed mismatch repair, was also unaffected by the presence or absence of DNA polymerase II.
Kow
Y W
YW
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405.
Faundez
G
G
Hays
S
S
Bonner
C A
CA
Goodman
M F
MF
Wallace
S S
SS
D010584:Bacteriophage phi X 174 / Q000235:genetics* / Q000528:radiation effects; D004249:DNA Damage; D004257:DNA Polymerase II / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D005798:Genes, Bacterial; D016296:Mutagenesis; D013014:SOS Response, Genetics; D014162:Transfection; D014466:Ultraviolet Rays
D004279:DNA, Viral; D004257:DNA Polymerase II