title: Phosphatidylinositol 3-kinase activity is important for progesterone-induced Xenopus oocyte maturation.
abstract: In somatic cells, phosphatidylinositol 3-kinase (PI3 kinase) is a critical intermediary in growth factor-induced mitogenesis. We have examined the role of this enzyme in meiotic maturation of Xenopus laevis oocytes. PI3 kinase activity was present in immunoprecipitates of the p85 subunit of PI3 kinase from immature oocytes and markedly increased following progesterone stimulation. Injection of bacterially expressed protein corresponding to the C-terminal SH2 domain of p85 (SH2-C) inhibited progesterone-induced PI3 kinase activation and meiotic maturation. Injection of protein corresponding to the N-terminal SH2 domain or the SH3 domain of p85 did not inhibit PI3 kinase activation or maturation. SH2-C did not inhibit oocyte maturation induced by c-mos RNA injection. In addition, radiolabelled SH2-C was used to probe oocyte lysates, revealing that a novel 200-kDa protein bound to SH2-C. This protein may be an important mediator of progesterone-induced lipid metabolism in oocytes.
authors.affiliation: Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143-0130.
authors.forename: A J
authors.initials: AJ
authors.lastname: Muslin
authors.forename: A
authors.initials: A
authors.lastname: Klippel
authors.forename: L T
authors.initials: LT
authors.lastname: Williams
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007700:Kinetics; D046911:Macromolecular Substances; D008845:Microinjections; D008970:Molecular Weight; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000302:isolation & purification / Q000378:metabolism*; D010957:Plasmids; D011374:Progesterone / Q000494:pharmacology*; D006656:Protamine Kinase / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D046911:Macromolecular Substances; D010446:Peptide Fragments; D010750:Phosphoproteins; D011374:Progesterone; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D006656:Protamine Kinase; D017871:Calcium-Calmodulin-Dependent Protein Kinases