D21S418E identifies a cAMP-regulated gene located on chromosome 21q22.3 that is expressed in placental syncytiotrophoblast and choriocarcinoma cells.
A partial cDNA (D21S418E) whose nucleotide sequence has no significant homologies with known mammalian DNA sequences was isolated from a human placental library. The cDNA hybridized with a 10-kb transcript present in term placenta. Messages of 10 and 7.5 kb were induced in BeWo and JEG-3 choriocarcinoma cells by treatment with 8-Br-cAMP. The mRNA was not detected in human brain, liver, lung, kidney, pancreas, heart, skeletal muscle, or myometrium. The D21S418E locus was assigned to a 3.5-Mb region of chromosome 21q22.3.
Kido
S
S
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104.
Sakuragi
N
N
Bronner
M P
MP
Sayegh
R
R
Berger
R
R
Patterson
D
D
Strauss
J F
JF
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002822:Choriocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015723:Gene Library; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009928:Organ Specificity; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis / Q000235:genetics*; D014327:Trophoblasts / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
D018076:DNA, Complementary; D009363:Neoplasm Proteins; D011257:Pregnancy Proteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP