Prevention of the induced synthesis and secretion of group II phospholipase A2 by brefeldin A.
Brefeldin A (BFA) has previously been shown to block protein secretion and to cause dismantling of the Golgi cisternae in many cultured cell lines. BFA was found to prevent the induced synthesis and secretion of 14 kDa group II phospholipase A2 (PLA2) in rat mesangial cells. Furthermore, BFA inhibited total protein synthesis although PLA2 appeared to be more sensitive to the effect of this compound than total protein synthesis assessed by amino acid incorporation. BFA was unable to block protein synthesis or PLA2 activity in the cell completely but secretion of enzymatic activity and PLA2 protein into the cell culture media was totally inhibited.
Sánchez
R M
RM
Centre for Biomembranes and Lipid Enzymology, Utrecht University, The Netherlands.
Vervoordeldonk
M J
MJ
Schalkwijk
C G
CG
van den Bosch
H
H
D000818:Animals; D020126:Brefeldin A; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D004790:Enzyme Induction; D005455:Fluorescent Antibody Technique; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D054467:Phospholipases A2; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D051381:Rats
D003517:Cyclopentanes; D011500:Protein Synthesis Inhibitors; D020126:Brefeldin A; D010741:Phospholipases A; D054467:Phospholipases A2