title: MchAI and MchAII, two class-II restriction endonucleases from Mycobacterium chelonei.
abstract: We have purified and characterized two class-II restriction endonucleases from the saprophyte Mycobacterium chelonei AMB 82. MchAI was an isoschizomer of NotI recognizing and cleaving at 5'-GC/GGCCGC. MchAI did not require Triton X-100 for activity and was fully active at concentrations over 10-200 mM KCl and MgCl2. MchAII was an isoschizomer of HaeIII recognizing and cleaving at 5'-GG/CC. MchAII was active in 10 mM Tris.HCl pH 8.0 containing MgCl2 at 10-200 mM, was strongly inhibited by NaCl at concentrations higher than 10 mM and was inactive at KCl concentrations lower than 250 mM.
authors.affiliation: Department of Biochemistry, University of Delhi South Campus, India.
authors.forename: S
authors.initials: S
authors.lastname: Shankar
authors.forename: A K
authors.initials: AK
authors.lastname: Tyagi
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D016926:Mycobacterium chelonae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases; C024403:GGCC-specific type II deoxyribonucleases