Zea mI, the maize homolog of the allergen-encoding Lol pI gene of rye grass.
Sequence analysis of a pollen-specific cDNA from maize has identified a homolog (Zea mI) of the gene (Lol pI) encoding the major allergen of rye-grass pollen. The protein encoded by the partial cDNA sequence is 59.3% identical and 72.7% similar to the comparable region of the reported amino acid sequence of Lol pIA. Southern analysis indicates that this cDNA represents a member of a small multigene family in maize. Northern analysis shows expression only in pollen, not in vegetative or female floral tissues. The timing of expression is developmentally regulated, occurring at a low level prior to the first pollen mitosis and at a high level after this postmeiotic division. Western analysis detects a protein in maize pollen lysates using polyclonal antiserum and monoclonal antibodies directed against purified Lolium perenne allergen.
Broadwater
A H
AH
Biology Department, University of North Carolina, Chapel Hill 27599-3280.
Rubinstein
A L
AL
Chay
C H
CH
Klapper
D G
DG
Bedinger
P A
PA
D000485:Allergens / Q000235:genetics*; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D018076:DNA, Complementary; D008129:Lolium / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D000485:Allergens; D052179:Antigens, Plant; D018076:DNA, Complementary; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; C083547:Zea mI protein, Zea mays