title: Mechanical properties of rat cardiac skinned fibers are altered by chronic growth hormone hypersecretion.
abstract: Chronic growth hormone (GH) hypersecretion in rats leads to increased isometric force without affecting the unloaded shortening velocity of isolated cardiac papillary muscles, despite a marked isomyosin shift toward V3. To determine if alterations occurred at the level of the contractile proteins in rats bearing a GH-secreting tumor (GH rats), we examined the mechanical properties of skinned fibers to eliminate the early steps of the excitation-contraction coupling mechanism. We found that maximal active tension and stiffness at saturating calcium concentrations (pCa 4.5) were markedly higher in GH rats than in control rats (tension, 52.9 +/- 5.2 versus 38.1 +/- 4.6 mN.mm-2, p < 0.05; stiffness, 1,105 +/- 120 versus 685 +/- 88 mN.mm-2.microns-1, p < 0.01), whereas values at low calcium concentrations (pCa 9) were unchanged. In addition, the calcium sensitivity of the contractile proteins was slightly but significantly higher in GH rats than in control rats (delta pCa 0.04, p < 0.001). The crossbridge cycling rate, reflected by the response to quick length changes, was lower in GH rats than in control rats (62.0 +/- 2.6 versus 77.4 +/- 6.6 sec-1, p < 0.05), in good agreement with a decrease in the proportion of alpha-myosin heavy chains in the corresponding papillary muscles (45.5 +/- 2.0% versus 94.6 +/- 2.4%, p < 0.001). The changes in myosin heavy chain protein phenotype were paralleled by similar changes of the corresponding mRNAs, indicating that the latter occurred mainly at a pretranslational level. These results demonstrate that during chronic GH hypersecretion in rats, alterations at the myofibrillar level contribute to the increase in myocardial contractility observed in intact muscle.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM) U 127, Hôpital Lariboisière, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Mayoux
authors.forename: R
authors.initials: R
authors.lastname: Ventura-Clapier
authors.forename: J
authors.initials: J
authors.lastname: Timsit
authors.forename: F
authors.initials: F
authors.lastname: Béhar-Cohen
authors.forename: C
authors.initials: C
authors.lastname: Hoffmann
authors.forename: J J
authors.initials: JJ
authors.lastname: Mercadier
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line / Q000378:metabolism; D005260:Female; D013006:Growth Hormone / Q000378:metabolism / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D007537:Isometric Contraction / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000032:analysis / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D012333:RNA, Messenger; D013006:Growth Hormone; D009218:Myosins; D002118:Calcium