Interleukin-8 is chemotactic for vascular smooth muscle cells.
The chemotactic activity of interleukin-8 for human aortic smooth muscle cells was investigated in Transwell cell culture chamber using a polycarbonate membrane with pores of 8 microns. Interleukin-8 stimulated the smooth muscle cell migration time and dose dependently in the range between 10(-11) and 10(-8) M. At 10 nM of interleukin-8, the number of migrated smooth muscle cells increased by 40-fold over the basal level, and this stimulation was almost completely abolished by anti-interleukin-8 antibody. Checkerboard analysis showed that the response of smooth muscle cell to interleukin-8 was chemotactic. These results suggest that interleukin-8 may play a role in the pathogenesis of arterial intimal thickening and atherosclerosis.
Yue
T L
TL
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939.
Mckenna
P J
PJ
Gu
J L
JL
Feuerstein
G Z
GZ
D001011:Aorta / Q000187:drug effects / Q000502:physiology; D001161:Arteriosclerosis / Q000209:etiology; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*
D002630:Chemotactic Factors; D016209:Interleukin-8