Assignment of the human FAU gene to a subregion of chromosome 11q13.
The FAU gene is the cellular homologue of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). FAU (for FBR-MuSV associated ubiquitously expressed gene) encodes the ribosomal protein S30 fused to a ubiquitin-like protein. A cosmid clone containing the human FAU gene was used for fluorescence in situ hybridization to metaphase chromosomes. The obtained localization to 11q13 was confirmed by hybridization against a panel of somatic cell hybrids containing different parts of chromosome 11 on a hamster background. FAU was then further mapped, both on a panel of radiation-reduced somatic cell hybrids designed to carry different parts of the 11q13 region and by pulsed-field gel electrophoresis. This fine mapping assigned FAU close to the skeletal muscle glycogen phosphorylase gene (PYGM), in a region that contains several oncogenes as well as the putative tumor suppressor genes MEN1 and ST3.
Kas
K
K
University of Antwerp, Department of Biochemistry, Wilrijk, Belgium.
Schoenmakers
E
E
van de Ven
W
W
Weber
G
G
Nordenskjöld
M
M
Michiels
L
L
Merregaert
J
J
Larsson
C
C
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis / Q000235:genetics; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008214:Lymphocytes; D009857:Oncogenes; D006005:Phosphorylases / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D009053:Sarcoma Viruses, Murine / Q000235:genetics; D014452:Ubiquitins / Q000235:genetics*
D005819:Genetic Markers; D012269:Ribosomal Proteins; D014452:Ubiquitins; C040469:ribosomal protein S30; D004247:DNA; D006005:Phosphorylases