title: Herpes simplex virus type 1 capsid protein, VP21, originates within the UL26 open reading frame.
abstract: The goal of experiments reported here was to identify the genes that encode capsid proteins VP21 and VP24 of herpes simplex virus type 1 (HSV-1). Capsids were isolated from infected cells and the proteins were separated by SDS-PAGE. N-terminal amino acid sequence analysis of partial CNBr digestion products, and of intact VP21, showed that it is encoded within the UL26 open reading frame (ORF) of HSV-1 beginning with codon 248 and probably extending to the end of the ORF (codon 635). Similar analysis of digestion products confirmed that VP24 is specified by codons 1 to 247 at the 5' end of the UL26 ORF. Each of the seven known capsid proteins has now been assigned to an ORF.
authors.affiliation: Department of Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh Medical Center, Pennsylvania 15261.
authors.forename: S
authors.initials: S
authors.lastname: Person
authors.forename: S
authors.initials: S
authors.lastname: Laquerre
authors.forename: P
authors.initials: P
authors.lastname: Desai
authors.forename: J
authors.initials: J
authors.lastname: Hempel
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics*