Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II).
A complete cDNA encoding the acrosin-trypsin inhibitor, HUSI-II, was used as a probe to isolate genomic clones from a human placenta library. Three clones which cover the entire HUSI-II gene were isolated and characterized. The exon-intron organization of the gene was determined and found to be identical to other known Kazal-type inhibitor-encoding genes. The striking similarity in the amino acid sequences which was found previously in HUSI-II and glycoprotein hormone beta-subunits, is neither reflected in codon usage nor in the exon-intron arrangement of the genes. A 1.8-kb segment 5' of the gene was sequenced. The analysis of this sequence showed that HUSI-II contains a G + C-rich region upstream from the transcription start point (tsp) which fulfills the criteria for a CpG island. Furthermore, in the first intron, a potential glucocorticoid-responsive element was found as a half-palindrome flanked by two CACCC elements. Determination of the tsp by S1 mapping revealed that HUSI-II has multiple tsp. Genomic Southern hybridization was used to show that HUSI-II is a single-copy gene. The localization of the gene to chromosome 4 was determined by hybridization of a 5' genomic fragment to the DNA of a panel of somatic hybrids between human and rodent cells.
Möritz
A
A
Department of Clinical Chemistry and Clinical Biochemistry, University of Munich, Germany.
Grzeschik
K H
KH
Wingender
E
E
Fink
E
E
D000176:Acrosin / Q000037:antagonists & inhibitors*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D006063:Chorionic Gonadotropin / Q000235:genetics; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D006023:Glycoproteins; D006801:Humans; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D000074162:Serine Peptidase Inhibitors, Kazal Type; D014158:Transcription, Genetic; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000235:genetics*
D006063:Chorionic Gonadotropin; D006023:Glycoproteins; C066238:SPINK2 protein, human; D000074162:Serine Peptidase Inhibitors, Kazal Type; D014359:Trypsin Inhibitor, Kazal Pancreatic; D000176:Acrosin