Cloning and sequencing of a gene encoding the beta polypeptide of Ca2+/calmodulin-dependent protein kinase IV and its expression confined to the mature cerebellar granule cells.
A cDNA encoding the beta polypeptide of Ca2+/calmodulin-dependent protein kinase IV (CaM kinase IV) was isolated and sequenced from a rat cerebellar cDNA library. By in situ hybridization histochemistry, we demonstrated the differential gene expression for alpha and beta polypeptides of CaM kinase IV in mature and developing rat brains using oligonucleotide probes specific for each polypeptide.
Sakagami
H
H
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
Kondo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry*; D016922:Cellular Senescence / Q000502:physiology; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D005815:Genetic Code; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar
D010446:Peptide Fragments; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517686:Camk4 protein, rat