title: A new approach to the immunocytochemistry of cAMP. Initial characterization of antibodies against acrolein-fixed cAMP.
abstract: A method is described to couple cyclic adenosine 3',5' monophosphate (cAMP) to a carrier protein by means of acrolein. Antibodies against this conjugate were raised in mice. These antibodies proved to be highly specific for acrolein-fixed cAMP in a gelatin model system. Slices (300 microns in thickness) from rat cerebral cortex were incubated in vitro and the dopaminergic control of adenylate cyclase activity was drug-manipulated. This manipulation was visualized by application of the cAMP-antisera on cryostat sections of the acrolein fixed slices.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: De Vente
authors.forename: J
authors.initials: J
authors.lastname: Schipper
authors.forename: H W
authors.initials: HW
authors.lastname: Steinbusch
mesh_terms: D000171:Acrolein; D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry; D000242:Cyclic AMP / Q000032:analysis*; D005780:Gelatin; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000171:Acrolein; D005780:Gelatin; D000242:Cyclic AMP