title: Skeletal muscle metabolic enzymes are altered by hyperbaric oxygenation treatments.
abstract: To test whether repeated HBO exposures would increase activity of skeletal muscle metabolic enzymes, 27 rabbits (3 groups) were exposed 90 min/day, 5 days/wk to either 100% O2 at 243 kPa (HBO), 100% O2 at 101 kPa (HIO), or 21% O2 at 101 kPa (CON). Four animals per group were killed after 2 wk treatment, and the remaining five per group were killed after 8 wk of treatment. Soleus, plantaris, and tibialis anterior muscles were removed, and the activities of adenylate kinase, alpha-glycerophosphate dehydrogenase, and citrate synthase were measured. After 8 wk there was no difference in enzyme activity between groups for either plantaris or tibialis anterior. In the soleus after 8 wk there was no difference between groups in adenylate kinase activity, but alpha-glycerophosphate dehydrogenase activity was 56% greater (P < 0.05) in HBO than in HIO and 50% greater than in CON, and citrate synthase activity in HBO was 24% greater (P < 0.05) than that in HIO and 36% greater than that in CON. Inasmuch as the soleus is a postural muscle, these results suggest that long-term HBO treatments can increase enzyme activity in an actively contracting muscle.
authors.affiliation: Department of Kinesiology, Louisiana State University, Baton Rouge 70803.
authors.forename: A G
authors.initials: AG
authors.lastname: Nelson
authors.forename: E G
authors.initials: EG
authors.lastname: Wolf
authors.forename: P O
authors.initials: PO
authors.lastname: Bradshaw
authors.forename: C M
authors.initials: CM
authors.lastname: Hearon
authors.forename: B
authors.initials: B
authors.lastname: Li
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D013997:Time Factors
chemical_list: D005993:Glycerolphosphate Dehydrogenase; D002950:Citrate (si)-Synthase; D000262:Adenylyl Cyclases